LOCUS       DBMDYA010000001        24856 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_670_length_24856_cov_1.667542, whole genome shotgun sequence.
ACCESSION   DBMDYA010000001 DBMDYA010000000
VERSION     DBMDYA010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 24856)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24856)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24856
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_24856_cov_1.667542"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="ACZPFK_00005"
     CDS             <1..418
                     /locus_tag="ACZPFK_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HIR1404722.1"
                     /translation="RTQIAIVVDEYGGTAGIVTMEDILEEIVGNIEDEFDNEEEEIRA
                     AEGGCVADGAADLEDIFTWFGREAPEQEGDEDFGSVSGLITSRLGRIPREGEEVSLEY
                     GGLLFRVLQVGERRVERVFCRLAPQPENTELEESRA"
     gene            415..966
                     /locus_tag="ACZPFK_00010"
     CDS             415..966
                     /locus_tag="ACZPFK_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1404723.1"
                     /translation="MSEAVVHRETMLRRETIFEGRVITVTRDEVRLENGAASTREVVR
                     HHGGACVAALTDDGQLYFVRQFRYAYERELLELPAGKLEAGEDPFPAAQRELSEEVGV
                     TADEYADLGVFLPTCGYCNEVIHLYAARGLHRTAQHLDPDEFVTVLTIPFAEAERMAL
                     AGEINDGKTIAAIFRLRSLKGWL"
     gene            1431..2186
                     /locus_tag="ACZPFK_00015"
     CDS             1431..2186
                     /locus_tag="ACZPFK_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR1404724.1"
                     /translation="MRLVERAKSILSDEALLAEASAKGRANFVTRCDTAVQDFLQREL
                     RTRWPQVQFLGEESACAPDFSGSVWVLDPVDGTANLMHHFQHSAVSLALVEAGQPVFG
                     VVYNPFLAETFTAQRGRGAYLNGAPLRVSTDVPAADSVIAVGTSPYDRQLADENFALF
                     RRLFERCGDLRRTGSAALDLAYVAAGRVEGYLERNLKPWDFAAGALLVREAGGRVSDF
                     AGQEPDFSQNCDLVAAGPGLHGELLRLAAVGPR"
     gene            3177..3857
                     /locus_tag="ACZPFK_00020"
     CDS             3177..3857
                     /locus_tag="ACZPFK_00020"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF009299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1404725.1"
                     /translation="MELMLDTANLQELDRYLADYPAVGVTSNPSILKKEGGVDLWEHL
                     RKVKALCGEDRSLHVQVVADSTPLILQEARRILAELGEETYIKIPVTPAGLPAVKSLS
                     EAGANVTATAVYTSLQGMMAVMAGAKYIAVYFGRIEKMGGDPERVIRELVAFIDNNGF
                     DAKVLAASFHEPQEVSRAYAAGAHSATVAPSVIEQGLSEPPMRRAVQDFTADFEAIHG
                     PGSNLTTV"
     gene            3907..4950
                     /locus_tag="ACZPFK_00025"
     CDS             3907..4950
                     /locus_tag="ACZPFK_00025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1404726.1"
                     /translation="MKILFIGGTGIISTAISERLLAQGHELWLLTRGTRKGLLAGARE
                     LTCDIRDEAAAAKLLEGQTFDAVADFIAYGPAEAERDFRLFAGKTRQYIFISSASAYQ
                     KPTALLPLTESTPLVNPYWDYSRQKAACEELLLRHLREDGFPVTIVRPSHTYCERKMP
                     VALHGEKGSWQVLARMLAGKPVVIHGDGSSLWTLTHSRDFAKGFCGLVGNAHALGQAV
                     QITSDESLTWNQIYGVIADALGVPLRAVYAPSSLLAEAGRAWGYDFEGGLLGDKSATV
                     LFDNAKLKRLVPGFAAVTRFDQGARETVAYLLAHPGLQQPDEKFDRFCDAVCAGMDAA
                     RLAIADAAQQPRN"
     gene            complement(5397..6974)
                     /locus_tag="ACZPFK_00030"
     CDS             complement(5397..6974)
                     /locus_tag="ACZPFK_00030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR1404727.1"
                     /translation="MAKKFVCTPHEPVVQTAQGALRGFILDGTYTFYGIPYAEAKRFQ
                     APRPVTPWQGVRDALSYGYVCPLMEQDAPDKEVLVPHRYWPMDEHCQNLNVWTRSLDP
                     AAKRPVMVWLHGGGFSAGSAIEHVAYEGDRLCEFGDVVVVSVNHRLNLLGYLDLSAFG
                     EQYANSANAGNADLVAALEWVRENIAAFGGDPDNVTLFGQSGGGEKVLTLMNTPAADG
                     LFHKGIIESGVTDLNAGGSGDGAPLVRAMLAWLGWDESRVAELETLPYPRLVEAYQAV
                     VARVAAAGGYIGCWPVANGWYAGNPRQVGFTEHAKTIPVLIGSVMGEFCFEPGVQDKY
                     GMSEAQVLPLLKEKYGLHAERLTELFRAAYPQKHLSDLLYLDAFFRAPTRQFIALRSE
                     TCAAPTYAYLFAYDFPIDGGKVAWHCSEIPFVFHNTCRTPLFNVPGETDRLEEQVCGA
                     FVNFARYGAPQICSLPAWPACAPGDEGVMVLDKSCEVKHNFDAALIDALTESGAAPAI
                     TLLEVVAPGAGTVTVRH"
     gene            complement(7040..8569)
                     /locus_tag="ACZPFK_00035"
     CDS             complement(7040..8569)
                     /locus_tag="ACZPFK_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404728.1"
                     /translation="MAKYTLIIADDEEIERKALRLLVQKEFPEIEIVAVADNGTELVA
                     QVQRHQPDIAIVDVNMPGINGIDAIDLLCARGSRTHFIINTAYDEFDYVQRALALKID
                     AYILKPEKRDTTIGTIRKLCAQIDEARANIQSQRQIQELFTRIQPVMESEIMYSLFIG
                     EPAAANFDAYCEMHAARFEAGAVAALIPVAEGRDGLRGQDKAALRTALDAAFGNSCTY
                     LATITETNICLLIFTPGGSAAERRRWLSDVLRVAMDKLNHSLRLPLRAGVGGIFGEFE
                     KMAASYQQSLLALTGPAGGGVAFYEPPGAQGAARREGAAQARALACLAREGNLQRIGA
                     ETTRMQPLLRGDRGAARQLWADVMQELRREAEENAPLRAQLDFSAPLREGLYQAVMLL
                     GAQPQEGTGAYVRQALQYIADNYARDISLDTVAEQIGISPFYLSRLFKAERGETFVEY
                     LTGVRMKAATRLAKETRLSIREIAERTGYGNPTYFCRVFKKYTGSTIGDLREQGRKKR
                     F"
     gene            complement(8633..10147)
                     /locus_tag="ACZPFK_00040"
     CDS             complement(8633..10147)
                     /locus_tag="ACZPFK_00040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1404729.1"
                     /translation="MRDNAVSERRRGSITSKYSKGYFLMLLVLLLVFAVSLFSGQSLS
                     RRYGRAIDELLDLNELFVNVETTNKYVYDYYLYLRPTSSETYDAEQSKTQGAVNAMME
                     RMKTAYSRDIVDLCYMVQTYLDQSGQLMQRLDDFVADGHAAAEDAGLDVSYAQTQSVI
                     SYINQSFKEIYSARLVTTQRTQAEIQRSWRRLLALQTGMLLVAVAICFGFYRKVVHGI
                     TLSVKKLTAFASGVARQPALQEHVQIDTGDELAVFADAFNEMIDTIHAQMDQIEADSR
                     MREQLQQAEMENLRISAALQSSQLKLLQARINPHFLFNTLNMISQTARMEEAEETARL
                     MEATADFLRYNLGKVTKAVTLADEIDNTRNYVYIQRCRFGERIRFVFEVDESCAGQEV
                     PCMILQPLVENSVTHGVGAMIGGGTVTIRLFKQDGAACLEVRDNGVGISAQRLAQLRD
                     SFKKEGGEGGHIGLKNVYLRLKLFFGEGLQFSIDSGPDGTAVRIRLPRSGPGPA"
     gene            complement(10137..11105)
                     /locus_tag="ACZPFK_00045"
     CDS             complement(10137..11105)
                     /locus_tag="ACZPFK_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1404730.1"
                     /translation="MKQNFLRVLVPFFALCLLAGCGTEKPGTTPESAPADWPLRISAI
                     LPHDDQGYWTTLGAGILERAGELEADAKIVTPQLNYNISQMIELIRQATAAQVDAIIV
                     QGIEDPDYLRALEDASDAGVQVVMVDTNVAETFPHLYVGTDNYAAGVQLGRELIRETD
                     GKAVVAVLTGAEGYPNLDARVRGLQDVIKEQPGIEIRRVEYDEYDSLTVMEKYNLILR
                     EDPDVDTIVCVEGTGGQTFGRLLTPDTCPLEHLLGFDLSDETLMGLSSGLIDGSLVQQ
                     QKEMGRMAVEELVRYFDGGAYRSMTLYTPTVYVTAEDLNEEGVYEG"
     gene            complement(11197..12207)
                     /locus_tag="ACZPFK_00050"
     CDS             complement(11197..12207)
                     /locus_tag="ACZPFK_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HIR1404731.1"
                     /translation="MLKEKKKFNLRSFLMNYALYAIMVLMIIVIAVISGGKFLSLRVL
                     RDILTQSASRIVVAMGCMFVIVSGSADLSGGRTVGLAAVISGSLAQQATYYLKFWPSL
                     PELPVIVPVLVSIAVGLLVGCFNGFVVSRLKVPAFLATLGTQMIVYGANCLYFNKAPN
                     NSQPLGGYLENFGKIGTGAIFGIPYLVIIAFVVMMLVWILQTKTCLGKEIFAVGGNPE
                     AARVSGINVFKIQMITYAVAGACYGLAGTLEAARTGSASSTYGLSYELDAIASCIVGG
                     CSAAGGVGTVPGVFFGVIIFNVINYGLTFIGLSSYWQFVVKGVIIIGAIAVDVRKYNN
                     KH"
     gene            complement(12223..13761)
                     /locus_tag="ACZPFK_00055"
     CDS             complement(12223..13761)
                     /locus_tag="ACZPFK_00055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1404732.1"
                     /translation="MEQTPVLEMSGISKSFPGVKALDNVSLTVRPGTVHALMGENGAG
                     KSTLMKCAFGLYHPDEGTIRVSGEEVHFVDARDAMDKGVSMIHQELHPIRTRNVMENI
                     WVGRVPYKRVGPVHWVDEKKMYNDTKALLEQLDVKLDPRTMVGELNASNCQMIEIVRA
                     VSYGAKVIIMDEPTSSLTEVEAEQLFKIIGQLTAQGVAIIYISHKMDEILRISDEVTI
                     MRDGKLVGTWPAGELTMQLMVSRMVGREMSNQFPPKDHTPSEEIMLEVKHLTSADPRS
                     FKDVSFRVRRGEIFGIGGLVGAQRTELMESVFGLRPVAGGEIGYKGEKAKIGCAQDAI
                     QLGMALLTEERRASGIIPILSVMENTVLANQTAHTRQYTGGKLFLNDKKRNEDAQRYV
                     EALSIKTPTLKTQIQYLSGGNQQKVLLGRWLLVNPDLLILDEPTRGIDVGAKYEIYTL
                     MEQMARDGKTVIMISSEMPELMGMSDRIMVMCEGHLSGILQGSEATDEKIMMLASTYE
                     NANV"
     gene            complement(13863..14978)
                     /locus_tag="ACZPFK_00060"
     CDS             complement(13863..14978)
                     /locus_tag="ACZPFK_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1404733.1"
                     /translation="MKKALSLVLACAMALSLVACGGSDSSSAAAGGSSAAAGGSGATA
                     ATATAGVCFYNFADTFIANARQSLENIAAADGAVKVTTADSQGAAETQDNNMNNFYTQ
                     GVDFMLLNNLKTNAKPELIEKAKSEGVTLMFINSDSPTDEEFAMYDKVYHVSSAAEQS
                     GKIMGDAMLEYINTHDDWDRNGNGTFDYIMLLGTQGNYDTIQRSERSVEVLANAGIKL
                     NCVGGEQLCEWSRATAQDKVAALLANYSEDIDGVFACNDDMALGAIEALKAGGFFTGD
                     DTYLPVCGVDATQVGCDAIREGTLLVTSMNNPVLLGKATYKTMKLLAAGEELTQENLG
                     LEGCTVDGHHVWLNYTAVNAENVDTVGYDINDVDISK"
     gene            15330..15833
                     /locus_tag="ACZPFK_00065"
     CDS             15330..15833
                     /locus_tag="ACZPFK_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="jacalin-like lectin"
                     /protein_id="HIR1404734.1"
                     /translation="MAVSTEDARSHEFANSVPDRDSVFFKFDDAEFARSHRRVCAVAL
                     YGGRVLDGFQFFYDDQVPGYHRGGTGGSRTLFTLAAGEVITEVRGSTGTDPFASAYPT
                     LGELTLVTNRGRKCSVGMGSGFSQQTPFCYRATPGCGILALKGACFGVSDGLLVTGLQ
                     VSLEAQA"
     gene            complement(16005..17138)
                     /locus_tag="ACZPFK_00070"
     CDS             complement(16005..17138)
                     /locus_tag="ACZPFK_00070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1404735.1"
                     /translation="MKSRAEYDKLLKSLSENAREIEVNGLTVEVRDCADRRPGRPDPR
                     SAAAARLTRGQFASRRSELDPELWANEDHLFGPQDPQHYSTLELLRFQFGWTSSDLST
                     GVRTLHRQVPGPRGPVSVYQYEVLSAAEDRPCMVFIHGGGFFGGVIPTVENQCKLLAQ
                     LSGGVVLSVEYPLCPEHKYPQGFDACYAAVEWAFAHAAELGVSRKKLCVSGDSAGGNL
                     SLACSLRDRDEGKGMIAYQALIYPTVSRLEKVGAAHYWKPELYENPENDPLIDEQIRV
                     IGSMGDECNGWYTETGEDLKSPYLSPLFGDPAGLPKTLLMTAEYDFLRAECEEYSRRL
                     CAAGVENRHIRYGGIFHGTFDRLGYAPQVEDMLREIAKDMRAL"
     gene            complement(17190..18032)
                     /locus_tag="ACZPFK_00075"
     CDS             complement(17190..18032)
                     /locus_tag="ACZPFK_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1404736.1"
                     /translation="MKYRSANIDEKGMLFGKWCSMPGVWTLTNRWQNFMFLIVGEEKA
                     MLIDTGYGEGNLREIVEGITDKPVMVVDTHGHFDHTGGNAWWTDVWMAEGAVAGARVP
                     FSPEQQAWYDAKPHRDYNIHLLKEGDLIDLGGKQIEVLSIPAHHEGSIALLDRTDRLL
                     FTGDEMESGQVLLFVRDRNLPLREVVAAHRANLQKLAARRAEYDFICPAHNGTLLQPD
                     LYLNDFLALDQEILDGTARVQPDTAGFGYAPYQDGDDLFSKMGGQERAEHGQAAIVYL
                     KPEE"
     gene            complement(19316..20005)
                     /locus_tag="ACZPFK_00080"
     CDS             complement(19316..20005)
                     /locus_tag="ACZPFK_00080"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1404737.1"
                     /translation="MIDNIGVLGAMPEEVALLTQRLEEPQAKTLAGVEFHTGRMAGRQ
                     VTVCCAGMGKVNAAAAAQLLITVFGAKALLFSGIAGNMTTRVGVGDMVISDVACYHDA
                     ELPMIAQSYPFLTEYPASPALVAAAEAACRAQGVRYTVGRVATGDLFIGDGETKRRIE
                     AACHPACVEMEGAAVAQVAAKNDLPFVILRTMSDNCDEAAREKLVEQQFDISDYCRTA
                     SAVTAAVIERV"
     gene            complement(20170..21270)
                     /gene="ychF"
                     /locus_tag="ACZPFK_00085"
     CDS             complement(20170..21270)
                     /gene="ychF"
                     /locus_tag="ACZPFK_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1404738.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITNAGAQSANYPFCTIEPNVGVVAV
                     PDARLDRLAEMYHPKKYTPATIEFVDIAGLVKGASKGEGLGNKFLSHIREVDAIIHVV
                     RSFDSTDVIHVEGSVDPARDIETIDTELILSDIEITERRLEKTKKAMKGGDKALAADA
                     AFLERLLAHLSDGKNARTLEVEEDGEKALFDDLHLLSAKPIIYAANLSEDDLLAGIGE
                     NPYYPVIRDIAAAEGAGCMPICAKTEEDIAGLTPAEKKEFLAELGLEASGLDNLIRES
                     YALLGLISFLTAGEPEVRAWTIKKGTKAPQAAGKIHSDFERGFIRAEIVAYDDLMTCG
                     SMAAAKEKGLVRSEGKEYVMQDGDVTLFRFNV"
     gene            21545..22249
                     /locus_tag="ACZPFK_00090"
     CDS             21545..22249
                     /locus_tag="ACZPFK_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1404739.1"
                     /translation="MDKTKILIVDDDRNICELLRLYLEKEGYETQLAYDGEQALERFA
                     ADKPGLILLDVMMPKIDGWEVCRRIRASSQVPVIMLTAKGETFDKVLGLELGADDYIV
                     KPFDTKEVVARIKAVLRRYAAGGEKEEDAQKLISYDKLSVDLTRYELKVNGKTVDAPP
                     KELELLYYLASHPNRVFNRDQLLDEVWGFEYYGDSRTIDVHVKRLREKLEGVSDQWSL
                     KTVWGVGYKFEVKDEA"
     gene            22288..23769
                     /locus_tag="ACZPFK_00095"
     CDS             22288..23769
                     /locus_tag="ACZPFK_00095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1404740.1"
                     /translation="MNKSISGRYFRTTALLLVSGITLLGLILMYFCVGYFRTESENTL
                     LTNVRSLRDNFSTVSMEDADQMKEHPYVEQAVRVVANATGSIFFVTDNYGKILYCSDS
                     SGAVDTSRAVSSKILNRAFSDGEYAELGELSGIYSHSYYTAGCPLINDSSRIIGYVFA
                     SRDAASLTSFLESIFSAFIISAAVMLLVSSILSIVLTNRLVNPLRRITAAAQTFGNGD
                     LSARAPVGGDDEVAQLAVTFNDMADKLEAVDASRQSFMGNIAHELRTPMTTIKGFVDG
                     MLDGTIPDDRRDHYLQIVSQEVGRLTRLTHSMLDITKLETGEYVVSAVDYDVWETVSS
                     ILFAREQQFIEHAIRLTGFNPVKVLVHADPDLVHQVLYNIVDNALKFTPDGGTIDISV
                     TENPKQGFVTVAIRNTGEGIPADALPYVFERFYKADKSRGLHAQGSGLGLHICKQLIH
                     RSDGRIWAESTPGQDATFLFTLPCGNPKPPERSKYARGGQGGK"
     gene            24262..24612
                     /locus_tag="ACZPFK_00100"
     CDS             24262..24612
                     /locus_tag="ACZPFK_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404741.1"
                     /translation="MTLGERIKTILAECGLKQVDFAAALGVSANYVNQLVNGKKETLS
                     GTLAKLIEELYGYPAQWVLTGEGEKAPEASFSAAKAELLKKVSKMPQDEILALFAFAE
                     SLESVKKRFPPQNP"
BASE COUNT         4311 a         7968 c         7757 g         4820 t
ORIGIN      
        1 gcgcacccag atcgccattg tggtggatga atacggcggt accgccggca tcgtcaccat
       61 ggaggatatc ctggaggaga tcgtgggcaa catcgaggac gagttcgaca atgaggagga
      121 ggagatccgc gccgccgagg gcggctgtgt ggccgacggc gcggcggatc tggaggatat
      181 ttttacctgg ttcggccgcg aggcccccga gcaggagggc gacgaggatt tcggctcggt
      241 gagcgggctg atcaccagcc gcctggggcg catcccgcgg gagggcgagg aggtctcgct
      301 ggagtacggc gggctgctgt ttcgggtgct gcaggtgggg gagcgccggg tggaacgggt
      361 gttctgccgc cttgcccccc agccggagaa tacagaactg gaggagagca gggcatgagc
      421 gaagctgtgg tacacagaga gacgatgctg cgccgcgaga ccatctttga gggccgggtg
      481 atcacggtga cgcgggacga ggtgcggctg gagaatgggg ccgcctccac ccgcgaggtg
      541 gtgcgccatc acggcggcgc ctgcgtggcc gcgctgaccg acgacggcca gctgtatttt
      601 gtgcggcagt tccgctatgc ctatgagcgg gagctgctgg agctgccggc aggcaagctg
      661 gaggcggggg aggacccctt tcccgccgcg cagcgggagc tgtcggaaga ggtgggggtg
      721 acggcggacg agtacgccga cctgggcgtt tttctgccca cctgcggcta ctgcaacgag
      781 gtcatccacc tctatgcggc ccgtgggctg caccgcaccg cccagcacct ggacccggac
      841 gagttcgtca ccgtgctcac catccccttt gccgaggcgg agcggatggc cctggcgggg
      901 gagatcaacg acggcaagac catcgcggcc atctttcggc tgcggtcgct caaaggttgg
      961 ctctaagtaa agcggcaacc tgcggcgcag cggccaactc gatacttacg cccgcagggc
     1021 ggacgtaagc tagcaagggc aatatcttcc gctgagggct gtaagcaaag cggcaacctg
     1081 cggcgcagcg gccaacctga tccttatgcc cgcacagtgg gcataagccg gcacgggcgt
     1141 ccttttccgt ccgcgcggcg ggagcatacc gatagttcaa gacacggcgg cacggctgac
     1201 agctcggacg ttttcagttc cgggccgggg cgacagcggt tcccccgcgg ccggcgatga
     1261 aaaaaccggc tcggcgcctt ttgcagcgac ccagggctgg tttattcaca ctccgcattt
     1321 ttgtcaaagc ggcggccacc ctgcccgcaa agcggggctc gtggggccgc tgccattttt
     1381 tctttttctg gaaagaggga ctttttctga aaatcgattt tgacgaggtc gtgcgcctgg
     1441 tggagcgcgc aaaaagcatc ctctccgacg aggcgctgct ggcggaggcc tccgccaagg
     1501 ggcgggccaa ttttgtcacc cgctgcgaca ccgccgtgca ggactttttg cagcgcgagc
     1561 tgcgcacccg ctggccgcag gtgcagtttt tgggagagga gtcggcctgc gcgccggatt
     1621 tttccggctc ggtgtgggtg ctggacccgg tggacggcac cgccaacctg atgcaccact
     1681 ttcagcacag cgccgtctcg ctggcgttgg tggaggcagg acagccggtt ttcggcgtgg
     1741 tctacaaccc ctttctggcc gagactttca ccgcgcagcg gggcaggggg gcctatctga
     1801 acggggcgcc gctgcgggtc agcacggatg tgcctgcggc ggacagcgtg atcgccgtgg
     1861 gcacttcccc ctacgaccgg cagctggcgg acgagaactt cgcgctgttc cgccggcttt
     1921 tcgagcgctg cggcgacctg cggcgcaccg gctcggcggc gctggacctg gcctatgtgg
     1981 ccgctggacg agtggaggga tatctggagc gcaacctgaa gccttgggat tttgccgcgg
     2041 gcgcgctgct ggtgcgtgaa gcggggggcc gcgtcagcga ttttgccggg caggaaccgg
     2101 atttttccca aaactgtgac ctggtcgccg ccgggcccgg cctccacggg gagctgctgc
     2161 gcctcgccgc agttggcccg cgctgagcgg gctccagcga gtatcaggcc gatcgctttt
     2221 tcggccgctg ctttctggcc ggagcccgga tcgtcttttg ctttttttct gcacaaaaaa
     2281 tcagccgccc ggctttcgcg gggcggctga ttgcttaaat catcatggga gtttaagcct
     2341 ttcctgattt catgagataa acttcgtgga tgttcgttaa gggatcactt gaaaacttac
     2401 aggtagaacc aattgcttta aaatggaatg atcttaaaac cgttggaaca aatgcctttc
     2461 ggtcctggtg aaagaactca caaacttccg tcgaacttca tctatctgaa aatttgggat
     2521 ccggcctctg caaggcggcg aggactcaat ttacgccgtt gcggtatatt ttggccaaca
     2581 ctcttgccgc tgatcatgca gcatacaaga ggcgcctgtc gtttttataa actataaaat
     2641 cacgcctgct taaattatgc tgccgccctg cagatgaaat ggccgggctc tccgtggtca
     2701 ctgcgttcta ctttgtgcac cggtctcacc tctttcctct cagtcgcggc atcgggcttt
     2761 gactttcatt gccggcaagt tctttattaa gaccctgcgg ccgtctcttt cccgtctgcc
     2821 ttagttcctt tctgtgtctt tattatagcc aatccaaagc tgtttttcta ttggcatttt
     2881 aaatggattt ttggcctttg atatgtacta taataccaat ttgtgacggt ggcatgccgt
     2941 tttttagact tttttgtaaa cgttctttgt tgaaaacttt tccctgccgc gcaccgggct
     3001 ccaaagtgcg gggccgggcg caaaaacatc ttttgacatc cccgtccctt ttgggtaaaa
     3061 tgcagttggc ggcgcatact gcactgcgcg ccccaccgcg tcccggcctg cgggcagaaa
     3121 gaggtatcgc cttttttctc cccctgccgg gcgctgcaag aaaggggaaa tgacagatgg
     3181 aactgatgct cgacaccgca aacctacagg agctggatcg gtatctcgcc gactatcccg
     3241 ccgtcggggt gaccagcaac ccctccatcc tgaaaaaaga gggcggggtg gacctttggg
     3301 agcatctgcg caaggtcaaa gcgctttgcg gtgaggaccg cagcctgcat gtgcaggtgg
     3361 ttgcggacag caccccgctg atcctgcagg aggcccgccg catcctcgcc gagctgggcg
     3421 aggagaccta tatcaagatc cccgtcaccc ccgcagggct gcccgccgtc aaatccctct
     3481 cggaggccgg cgccaacgtc accgccacgg cggtgtacac cagcctgcag ggcatgatgg
     3541 ccgtcatggc cggggcaaaa tatatcgccg tctatttcgg ccgcatcgaa aagatgggcg
     3601 gcgacccgga gcgggtcatc cgtgagctgg tggcctttat cgacaacaac ggctttgacg
     3661 caaaggtgct ggcggccagc ttccatgagc cgcaggaggt gtcccgcgcc tacgccgccg
     3721 gcgcccacag cgccaccgtg gcccccagtg tgatcgagca gggcctctca gagccgccca
     3781 tgcgcagggc tgtgcaggat ttcaccgccg actttgaggc gatccacggg ccgggcagca
     3841 acctgaccac tgtttgagcg cccctgcgca aacacgacaa accggaaagg acaaacaggt
     3901 gacgatatga aaatcctctt tatcggcggc accggcatca tcagcaccgc catcagcgag
     3961 cggctgctgg cgcaggggca cgagctgtgg ctgctcacgc gcggcacccg caagggcctg
     4021 ctggccggcg cgcgggagct gacctgtgac atccgcgacg aggccgcggc ggcaaagctg
     4081 ctggaggggc agacgtttga cgcggtggcc gactttatcg cctacggccc ggcggaggcg
     4141 gagcgggact ttcgcctttt cgccggaaaa acgcggcagt acattttcat cagctccgcc
     4201 tcggcgtacc aaaagcccac ggcgctgctg cccctcaccg agagcacgcc gctggtcaac
     4261 ccctattggg actactcccg ccagaaagcg gcctgcgagg agctgctcct gcgccacctg
     4321 cgcgaggacg gcttcccggt gaccatcgtg cgccccagcc acacctactg cgagcgcaag
     4381 atgccggtgg ccctgcacgg cgaaaagggg agctggcagg tgctggcccg catgctggcg
     4441 ggcaagccgg tggtcatcca cggcgacggc agctcattgt ggaccctgac ccacagccgc
     4501 gactttgcca agggcttctg cggcctggtg ggcaacgccc acgccctggg acaggcggtg
     4561 cagatcacct cggacgagag ccttacctgg aaccagatct acggcgtgat cgcggacgcg
     4621 ctgggggtgc cgctgcgggc ggtctatgcg cccagcagcc tgctggcgga ggcgggccgg
     4681 gcctggggct acgactttga gggcggcctc ttgggggaca agtcggccac cgtgctgttc
     4741 gacaacgcca agctcaagcg gctggtgccc ggttttgccg ccgtcacccg ctttgaccag
     4801 ggggcgcggg agaccgtcgc ttatctgctc gcccaccccg ggctgcagca gccggacgaa
     4861 aaattcgacc gcttttgcga cgcggtctgc gcgggcatgg acgccgcccg cctcgccatc
     4921 gccgacgccg cgcaacagcc gcgcaattga ggtacaaaag gaccaggcgc cgatagcggc
     4981 ggctggtcct ttttgttctc cgccccggtt gccggtgggc ggcggaagaa agccccttag
     5041 aagaaagccc cttaaaataa gagccctctc cccgcgaaag taagcgctct cagacggcct
     5101 ttgaccgccg gttcccttga gaggggagcc aacgagtgcg ccgcatgcga ggagtggaac
     5161 ttgaaaggtg cgtcttgctc ctatcaaggc aaaaggccca aagcgcgcgc cgcttgcttt
     5221 tgcttgacaa gcgaagccat ctcgcccccg acggccgcgc ggagagcacg ccgacgtcgt
     5281 cctcatcacc atcgcccggc aaaagctgtc ccacagcaat aaaagagcag ccggtgttgc
     5341 cggctgctct ttttgcgttt tcacattgcg cttttgcgcc ccgtcgccgt tgagcctcaa
     5401 tggcgcacgg tgacggtgcc cgcgccgggg gccaccacct ccaacagggt tatggccggc
     5461 gccgcgccgc tctcggtcaa agcgtcgatc agcgccgcgt caaaattgtg ctttacctcg
     5521 cagctcttat ccagcaccat gacgccctcg tcgccgggcg cgcaggcggg ccaggcgggc
     5581 agcgagcaaa tttgtggggc gccgtaacgg gcaaaattca cgaacgcgcc gcaaacctgc
     5641 tcctccaggc ggtcggtctc gccgggcaca ttgaacagcg gcgtgcggca ggtgttgtgg
     5701 aacacgaacg ggatctcgct gcagtgccag gccaccttgc ccccgtcgat gggaaaatcg
     5761 taggcaaaca gataggcgta ggtcggcgcc gcgcaggtct cgctgcgcag ggcgatgaac
     5821 tggcgggtgg gcgcgcggaa aaacgcgtcc agatacagca aatcggacag gtgcttttgc
     5881 gggtaagcgg cgcggaagag ctcggtcagc cgctccgcat ggaggccgta cttttctttc
     5941 agcaggggca gcacctgggc ctcgctcatc ccgtacttgt cctgcacgcc gggctcaaag
     6001 cagaattccc ccatcacgct gccgatcagc accgggatgg tctttgcgtg ctcggtgaag
     6061 cccacctggc gcgggttgcc cgcataccag ccgttcgcca ccggccagca gccgatatag
     6121 ccgccggcgg cggccacccg ggccaccacc gcctgatagg cttccaccag gcgcgggtag
     6181 ggcagggtct ccagctccgc cacccggctc tcgtcccagc ccagccaggc gagcatggcc
     6241 cgcaccagcg gcgcgccgtc gcccgagccc ccggcgttca ggtcggtgac gccgctctcg
     6301 atgatcccct tgtggaacag cccgtccgcc gccggggtgt tcatcagcgt gagcaccttt
     6361 tcgccgccgc cggactggcc gaacagggtc acgttgtccg ggtcgccgcc aaaggcggcg
     6421 atgttctcgc gcacccattc cagcgcggcc accaggtcgg cgttgcccgc gttggcggaa
     6481 ttggcgtatt gttccccaaa ggccgacagg tccagataac ccagcaggtt cagccggtgg
     6541 ttcaccgaga ccaccaccac gtcgccgaac tcacacagcc ggtccccctc gtaggccacg
     6601 tgctcgatgg cgctgccggc cgaaaagccg ccgccgtgca gccagaccat gaccggccgc
     6661 tttgcggccg ggtccaggct gcgggtccaa acgttcaggt tctggcagtg ctcgtccatg
     6721 ggccaatagc ggtggggcac cagcacctcc ttatccggcg cgtcctgctc catcaggggg
     6781 cagacatagc cgtagctcag cgcgtcccgc accccctgcc agggcgtgac cggccggggc
     6841 gcctgaaagc gctttgcctc tgcgtagggg atgccgtaaa aggtgtaggt gccgtccagg
     6901 ataaagccgc gcagcgcccc ctgcgcggtc tgcacgaccg gctcatgggg ggtacagaca
     6961 aatttttttg ccatcttaaa ctcttctttc tctgaatgat gttgaggtgg gcccgcccct
     7021 tacgggcagg ggcccggggt caaaaccgtt ttttgcgccc ctgctcccgc aggtcgccga
     7081 tggtgctgcc ggtatatttt ttgaagacgc ggcagaaata ggtggggttg ccgtagccgg
     7141 tgcgctcggc gatctcacgg atcgagagcc gcgtctcctt tgccagccgg gtggcggctt
     7201 tcatccgcac cccggtgaga tattccacaa aggtctcgcc ccgctccgcc ttgaacaggc
     7261 ggctgaggta aaaggggctg atgccgatct gctcggccac cgtgtccagc gagatgtccc
     7321 gcgcgtagtt gtccgcgatg tactgcagcg cctggcgcac gtaggcgccg gtgccctcct
     7381 gcggctgggc gcccagcagc atcaccgcct ggtaaaggcc ctcgcgcagc ggggcggaaa
     7441 aatccagctg tgcccgcagg ggcgcgttct cctccgcctc gcggcgcagc tcttgcatca
     7501 cgtccgccca gagctgccgc gcagctcccc ggtcgccccg cagcaggggc tgcatccgcg
     7561 tcgtctcggc cccgatgcgc tgcaggttgc cctcgcgcgc caggcaggcc agggcccgcg
     7621 cctgcgccgc gccctcgcgc cgggcggcgc cctgcgcgcc cgggggctcg taaaaggcca
     7681 ccccaccgcc cgcggggccg gtcagggcca gcaggctctg ctggtaggag gcagccatct
     7741 tttcaaactc gccgaagatg ccgcccaccc ccgcgcgcag gggcagccgc aggctgtggt
     7801 tcagcttgtc catggccacc cgcagcacgt ccgacagcca gcggcgccgc tcggcggcgc
     7861 tgccgccggg cgtgaagatc agcaggcaga tgttggtctc ggtgatggtg gcgagatagg
     7921 tgcagctgtt gccgaacgcc gcgtccagcg ccgtgcgcag cgccgccttg tcctggccgc
     7981 gcaggccgtc gcgcccctcc gccaccggga tgagcgccgc caccgccccg gcctcgaacc
     8041 gcgccgcgtg catctcgcag taggcgtcga agtttgcggc cgcgggctcg ccgatgaaga
     8101 gggaatacat gatctcgctc tccatcaccg gctggatgcg ggtgaacagc tcctggatct
     8161 ggcgctggct ctggatgttg gcccgcgcct cgtcgatctg ggcgcagagc ttgcggatgg
     8221 tgccgatggt ggtgtcccgc ttttccggct tgaggatata ggcgtcgatc ttgagcgcca
     8281 gcgcccgctg cacatagtcg aactcgtcgt aggcggtgtt gatgatgaag tgggtgcggc
     8341 tgccccgggc gcagagcagg tcgatggcgt cgatgccgtt gatgcccggc atgttcacgt
     8401 ccacgatggc gatatccggc tggtgccgct gcacctgcgc caccagctcg gtgccgttgt
     8461 ccgcgacggc gacgatctcg atctcgggaa actccttttg caccagcagc cgcagcgcct
     8521 tgcgctcgat ctcctcgtcg tccgcgatga tgagcgtata ctttgccatt ttaccccctc
     8581 ctctcccttt tttgcgcttt gggcccgcgg cgggcgtccg cccccgccgc cctcaggccg
     8641 ggccgggccc cgagcggggc agcctgatgc gcacggccgt gccgtccggc ccgctgtcga
     8701 tggagaactg cagcccctcg ccgaagaaca gcttgagccg cagatagacg tttttcaggc
     8761 cgatgtggcc gccctcgccg ccctcttttt tgaaagaatc ccgcagctgg gcaaggcgct
     8821 gggcgctgat gcccacgccg ttgtccctca cctccagaca ggccgcgccg tcctgtttga
     8881 acagccgtat cgtgaccgtc ccgcccccga tcatggcgcc caccccgtgg gtgactgaat
     8941 tttccaccag cggctgcagg atcatacagg gcacctcttg gccagcgcag ctctcgtcca
     9001 cctcaaacac aaagcggatc cgctcgccaa acctgcaccg ctggatatag acatagttgc
     9061 gggtgttgtc gatctcgtcc gccagggtca ccgccttggt caccttgccc aagttgtacc
     9121 gcagaaaatc cgccgtggcc tccatcagcc gcgccgtctc ctcggcctcc tccatgcggg
     9181 cggtctggga gatcatgttc agcgtgttga agagaaaatg cgggttgatg cgggcctgca
     9241 gcagcttgag ctggctggac tgcagcgcgg cgctgatgcg caggttttcc atctccgcct
     9301 gctgaagctg ctcccgcatg cgggagtcgg cctcgatctg gtccatctgg gcgtggatgg
     9361 tgtcgatcat ctcgttaaag gcgtcggcaa acacggccag ctcgtcgccg gtgtcgatct
     9421 gcacatgctc ctgcagggcg ggctgccggg ccacgccgga ggcaaacgcg gtcagctttt
     9481 tcaccgacag ggtgatgccg tgcaccacct tgcggtaaaa tccaaagcag atggccaccg
     9541 ccaccagcag catgccggtc tgcagcgcca acagccggcg ccaactgcgc tggatctcgg
     9601 cctgggtccg ctgggtggtc accagtcggg cggaatagat ctctttaaag ctttggttga
     9661 tataggagat aacgctctgc gtctgggcgt aggacacgtc cagacccgcg tcctccgccg
     9721 cggcgtgccc gtcggcgaca aagtcgtcca accgctgcat cagctgcccg ctttggtcca
     9781 ggtaggtctg caccatatag cacaggtcga cgatatcgcg ggaataggcc gtcttcatgc
     9841 gctccatcat ggcgtttacg gcgccctggg tcttcgattg ctccgcatca taggtctcgc
     9901 tgctggtggg gcgcagatag aggtaatagt cgtagacgta tttgttcgtg gtctcgacgt
     9961 tcacaaacag ctcgttcaaa tccagcagct cgtcgatggc ccggccgtac cggcgggaga
    10021 ggctctggcc gctgaacagg gaaaccgcaa acaccaacag cagcaccagc agcatcaaaa
    10081 aatagccttt gctgtatttg gaggtgatgg acccgcggcg gcgctccgag acggcattat
    10141 ccctcataga cgccctcctc gttcaggtcc tccgccgtga cgtaaacggt gggggtgtaa
    10201 agggtcatcg agcgataggc gcccccgtcg aaatagcgga ccagttcttc caccgccatg
    10261 cgccccatct ccttttgctg ctgtaccaga ctgccgtcga tcaggccgct gctcagcccc
    10321 atcagggtct cgtccgaaag gtcgaatccc aacaggtgct ccaagggaca ggtgtcgggg
    10381 gtgagcagcc gtccaaaggt ctggccgccc gtgccctcca cacagacgat ggtgtccacg
    10441 tccgggtcct cccgcaggat caggttgtat ttttccatca cggtgaggga gtcgtattcg
    10501 tcatactcca cccgtcggat ctcaatgccg ggctgctctt ttatcacgtc ctgcagcccc
    10561 cgcacccgcg cgtccaggtt ggggtatccc tcggcgcccg tgagcaccgc caccaccgct
    10621 ttgccgtcgg tctcgcggat cagctcccgc cccagctgca cgccggcggc gtagttgtcg
    10681 gtgcccacat acagatgggg aaaggtctcg gccacgttgg tatccaccat aaccacctgg
    10741 acgccggcgt ccgaggcgtc ctccagcgcc cgcagatagt ccgggtcctc gatgccctgc
    10801 acgatgatcg cgtccacctg ggcggccgtg gcctggcgga tcagctcgat catctgggag
    10861 atgttgtaat tcagctgcgg cgtgacgatc ttcgcgtccg cctccaattc cccggcccgt
    10921 tccaggatac cggccccgag ggtcgtccag tagccctggt cgtcatgggg cagaatagcg
    10981 gaaatgcgca gcggccaatc ggccggtgcg ctttccgggg tggtaccggg cttttccgtc
    11041 ccgcatcccg ccagcaagca aagggcgaaa aaggggacaa gcaccctcaa aaaattttgt
    11101 ttcaaaccgc cacactcctt tgggaactgt ctgtccttat ccccattgta aagggagcgc
    11161 ccggtttttg caatttttat ccctcccgag aggggctcag tgcttgttgt tgtacttgcg
    11221 cacgtccacg gcgatggcgc cgatgatgat cacgcccttg accacgaact gccagtagct
    11281 ggacagcccg ataaaggtca ggccgtaatt gatgacgttg aagatgatga cgccgaagaa
    11341 cacgcccggc accgtgccca cgccgccggc agccgagcag ccgcccacga tacaggaggc
    11401 gatggcgtcc agctcatagc tgaggccgta ggtggaagag gcgctgcccg tgcgggcggc
    11461 ctccagcgtg ccggccaggc cgtagcaggc gccggcgacg gcgtaggtga tcatctggat
    11521 cttaaacacg ttgatgcccg agacccgggc cgcctccggg ttgccgccca cggcgaagat
    11581 ctctttgccc aggcaggtct tggtctgcag gatccagacc agcatcatca cgacaaaggc
    11641 gatgatgacc agatagggga tgccgaagat ggcgccggtg ccgatcttgc cgaagttttc
    11701 aagataaccg cccaggggct gcgagttgtt cggcgccttg ttgaaataca ggcagttggc
    11761 gccgtagacg atcatctggg tgcccagcgt ggccagaaag gcgggtacct tcaggcggga
    11821 gaccacaaag ccgttgaagc agcccaccaa aaggcccacg gcgatggaga ccagcacggg
    11881 cacgatgacc ggcagctcgg gcagggaggg ccagaacttc aggtaatagg tcgcctgctg
    11941 ggcgagcgag ccggagatga cggccgccag gcccaccgtg cggccgccgg acaggtcggc
    12001 ggagccggag acgatgacga acatgcagcc catcgccacc acgatgcggg aggcggactg
    12061 ggtgaggatg tcgcgcagca cccgcagcga caaaaacttg ccgccgctga tgaccgcgat
    12121 gacgatgatc atcagcacca tgatggcgta cagagcatag ttcatcagga aagagcgcag
    12181 gttaaatttt ttcttctctt tcaacatgag agttttcctc ctttagacgt tggcgttctc
    12241 ataggtgctg gcgagcatca tgatcttttc atccgtggcc tcgctgccct gcaggatgcc
    12301 ggacagatgc ccctcgcaca tcaccatgat ccggtccgac atgcccatca gctccggcat
    12361 ttcggagctg atcatgatca cggtcttgcc gtccctggcc atctgctcca tcagggtgta
    12421 gatctcgtac ttggccccca cgtcgatgcc gcgggtgggt tcgtccagga tcagcaggtc
    12481 cgggttcacc agcagccagc gccccagcag cactttctgc tggttgccgc ccgacaggta
    12541 ctggatctgg gtcttgaggg tgggcgtttt gatgctcagc gcctccacat atctctgggc
    12601 gtcctcattg cgctttttgt cgttgagaaa cagcttgccg ccggtatact ggcgggtgtg
    12661 cgcggtctgg ttggccaaaa ccgtgttctc catcaccgag agaatgggga tgatgcccga
    12721 ggcgcggcgc tcttcggtga gcagcgccat gcccagctga atggcgtcct gggcgcagcc
    12781 gatctttgcc ttttcgccct tgtacccgat ctcgcccccc gccaccgggc gcaggccgaa
    12841 gaccgactcc atcagctcgg tgcgctgggc gcccaccagg ccgccgatgc cgaagatttc
    12901 ccccctgcgc acccggaagc tcacatcctt gaagctgcgc gggtcggcgc tggtgaggtg
    12961 tttcacctcc agcatgatct cctcggaggg agtgtggtct ttcggcggga actggttgct
    13021 catctcgcgc cccaccatgc ggctgaccat cagctgcatg gtcagctcgc cggcgggcca
    13081 ggtgcccacc agcttgccgt cgcgcatgat ggtgacctcg tccgagatgc gcagaatctc
    13141 gtccatcttg tgggagatgt agatgatggc gacgccctgt gcggtcagct gcccgatgat
    13201 cttgaacagc tgctccgcct ccacctcggt cagggaggag gtgggctcgt ccatgatgat
    13261 gaccttggcc ccatagctga ccgcgcgcac gatctcgatc atctggcagt tggacgcgtt
    13321 cagctcgccc accatggtgc gggggtccag cttgacatcc agctgctcca aaagggcttt
    13381 ggtgtcgttg tacatctttt tttcatcgac ccagtgcacc gggcccaccc gtttgtaggg
    13441 tacgcgcccc acccagatgt tctccatgac attgcgggtg cggatggggt gcagctcctg
    13501 gtggatcatc gagacgccct tgtccatggc gtccctggcg tccacaaaat gcacttcctc
    13561 cccgctgacc cggatcgtgc cctcgtcggg gtggtacagg ccgaacgcgc atttcatcag
    13621 ggtggacttg cccgcgccgt tctcccccat cagggcatgc accgtgccgg ggcgcacggt
    13681 cagtgaaaca ttgtccagcg cttttacgcc ggggaagctt ttgctgatac cgctcatttc
    13741 aagaaccggt gtttgttcca ttttggcctc ccgttccgat cacttcaaaa cagccgagcc
    13801 gcgttcttca ccggcccggc tgcttgtgtg atctgattta tttctttgtc gctctgtgcc
    13861 gattacttgc tgatgtccac atcgttgatg tcatagccca cggtgtcgac gttctcggcg
    13921 ttgactgcgg tgtagttgag ccagacgtgg tgaccgtcaa ccgtgcagcc ctccaggccg
    13981 aggttctcct gggtcagctc ttcgccggca gccagcagct tcatggtctt gtaggtggcc
    14041 ttgcccagca gcacggggtt gttcatggag gtgaccagca gggtgccctc gcggatcgcg
    14101 tcgcagccca cctgggtggc gtcgacgccg cagaccggca gataggtgtc gtcgccggtg
    14161 aagaagccgc cggccttcag ggcctcgatg gcgcccaggg ccatgtcgtc gttgcaggcg
    14221 aagacgccgt cgatgtcctc gctgtagttg gccagcagag ccgcgacctt gtcctgtgcg
    14281 gtggcacggc tccactcgca gagctgctcg ccgccgacgc agttcagctt gatccccgcg
    14341 tttgccagca cctccacgct gcgctcagag cgctggatgg tgtcgtagtt gccctgggtg
    14401 cccagcagca tgatgtagtc gaaagtgccg ttgccgttgc ggtcccaatc gtcgtgggtg
    14461 ttgatgtact ccagcatggc gtcgcccatg atcttgccgg actgctccgc ggcggaggag
    14521 acatggtaga ccttgtcgta catggcgaac tcctcgtcgg tgggggagtc ggagttgatg
    14581 aacatcaggg tcacgccctc gctcttggct ttctcgatca gctcgggctt ggcgttggtc
    14641 ttcaggttgt tgagcagcat gaagtcgacg ccctgggtgt agaagttgtt catgttgttg
    14701 tcctgggtct cggcggcgcc ctggctgtcc gcggtggtca ccttgaccgc cccgtctgcg
    14761 gcggcgatgt tctccaggct ctggcgggcg ttggcgataa aggtatccgc gaagttgtag
    14821 aagcagacac ccgcggtggc ggtcgccgcg gtggcgccgg accccccggc cgccgcgctg
    14881 gagccgccgg ccgccgcaga agagctgtca gagccgccgc aggccaccag gctgagcgcc
    14941 atggcgcagg ccaggacaag agacaatgct tttttcattt tgctttcctc ctgaaaatga
    15001 tcgtttcttc ttttcttccc gagggagcgg tgcgtccccc gcttgcaaga ccaagtataa
    15061 gggatggctg cccgcgccgg gaattcaaac attttgttaa caagatacat tttttgtggt
    15121 gccggatact ttttttgcga tttgtttact tttcttgcgc tcgccgtttc tcgcctctcc
    15181 tttgaatggg gaaagggtct tccgtgtgcc tgtctgaaat atcacaaaaa tctggatgaa
    15241 aaaaggcttt tatgatactt tttgactttt attccaaaaa atggtagtgt aaagaaaaca
    15301 tccacccttt gtctaaagga ggcaaaaaaa tggcagtttc aactgaagac gcccgcagtc
    15361 acgagttcgc caactccgtg cccgaccgcg attccgtgtt cttcaaattc gacgacgcag
    15421 aatttgcccg ctcccaccgg cgggtctgtg cggtggcgct ctacggcggc cgggtgctgg
    15481 acgggttcca gttcttctac gacgaccagg tccccggcta ccaccggggc ggcacgggcg
    15541 ggagccgcac cctcttcacc ctggccgcgg gggaggtcat caccgaagtc cgcggctcca
    15601 ccggcaccga tcctttcgcc tcggcctacc ccaccctggg cgagctcaca ctggtgacca
    15661 accgcggccg caaatgctcg gtgggcatgg ggtcgggctt cagccagcag accccgttct
    15721 gctaccgggc aaccccaggc tgtgggatcc tggctctgaa aggggcctgc ttcggcgtca
    15781 gcgacggcct tttggtcact ggtctccagg tcagcctgga ggcgcaggcc tgagcctcag
    15841 ccgtcgttcc cgtcctccgt tcctccaaaa aagacgcccc gggagcaatg ctcccggggc
    15901 gtcccgcttt taatatacga aaataaaccc acaggccggt aggttatggc cgcccggggc
    15961 ggcggccggc attcttcccg gcccttcgtc tcccggcagg cggatcacag cgccctcatg
    16021 tcctttgcga tctcccgcag catgtcctcc acctgcggcg catagcccag tcggtcaaag
    16081 gttccatgga agatgccgcc gtagcggata tgccggttct ccaccccggc ggcgcagagc
    16141 ctgcgggaat actcctcaca ctcggcccgc aaaaagtcgt actcggcggt catcagcagc
    16201 gttttcggca gccccgccgg gtcgccgaag agggggctga ggtaggggct tttcaggtcc
    16261 tcgcccgtct ccgtgtacca gccgttgcac tcgtccccca tcgagccgat gacccggatc
    16321 tgctcgtcga tgagcgggtc gttctcgggg ttttcgtaga gctccggctt ccagtaatgg
    16381 gcggcgccca ccttttccag ccgggagacg gtgggataga tcagcgcctg ataggcgatc
    16441 atgcccttgc cctcgtcccg gtcccgcagc gagcaggcca gcgagaggtt gccccccgca
    16501 gaatcccccg agacacagag ctttttgcgg ctcacgccca gctcggccgc gtgggcaaag
    16561 gcccactcca ccgcggcgta acaggcgtca aagccctgcg gatacttgtg ctcggggcag
    16621 aggggatact ccaccgacag aaccaccccg cccgagagct gagccagcag cttgcactgg
    16681 ttttccaccg tggggatgac cccgccgaaa aagccgccgc catggataaa caccatgcag
    16741 ggccgatcct cggctgcgga gagcacctcg tattggtaaa cgctcaccgg cccgcggggc
    16801 cccggcacct gccggtgcag cgtgcgcacg ccggtggaca ggtcgctgct ggtccaaccg
    16861 aactgaaagc gcagcagctc cagcgtggaa tagtgctgcg ggtcctgcgg cccgaacaga
    16921 tggtcctcgt tggcccagag ctccgggtcc agctcgctgc ggcgggaggc gaactggccc
    16981 cgggtgagcc gggccgccgc cgcgctgcgc gggtcgggcc gccccggccg acggtcggcg
    17041 cagtcccgca cctcgaccgt caggccgttc acctcgatct ccctcgcgtt ttcggacagg
    17101 cttttcagca gcttgtcata ttccgctctg ctcttcatca cgacaactcc tttttatatc
    17161 cctgggttgg atgcgccggg gaggataggc tattcctccg gcttcaaata cacgatggcc
    17221 gcctgcccgt gctcggcgcg ctcctgcccg cccatctttg agaacagatc gtccccgtcc
    17281 tgataggggg cgtagccgaa gcccgccgtg tcgggctgca cccgcgccgt gccgtccaga
    17341 atctcctggt ccagggcgag aaaatcgttc aggtagaggt cgggctgcag cagcgtgccg
    17401 ttgtgggcgg ggcagatgaa atcgtattcc gcccggcggg ccgccaactt ttgcaggttg
    17461 gcccggtggg cggcgaccac ctcgcgcagg ggcaggttgc gatcccgcac aaacagcagc
    17521 acctggccgg actccatctc gtcgccggtg aacagcagcc ggtcggtgcg gtccaacagg
    17581 gcgatgctgc cctcgtgatg ggccgggatg gagagcacct cgatctgctt gccgcccagg
    17641 tcgatgaggt cgccctcttt caaaagatgg atgttgtagt cccggtgggg ctttgcgtcg
    17701 taccaggcct gctgctcggg cgagaacggg acgcgggcgc cggccaccgc gccctcggcc
    17761 atccagacgt cggtccacca ggcgttgccg ccggtgtggt cgaaatggcc gtgggtgtcc
    17821 accaccatca ccggcttgtc ggtgatgccc tccacgatct cgcgcaggtt gccctcacca
    17881 tagccggtgt cgatgagcat cgctttctcc tcgcccacga tcaaaaacat gaagttctgc
    17941 cagcggttgg tcagggtcca caccccgggc atgctgcacc atttgccgaa cagcatcccc
    18001 ttttcgtcga tgttggcgct gcggtatttc acttttccac gcttcctttc tgccgccgcg
    18061 gcgcaaaccg cggcctgttt tcaagttcca tcctagcaca gcgccgcccc agcagtcgat
    18121 ttcaaatttt tgcactgaga ttatctttat ttgcaatttg cagaatttgc aaaatccaag
    18181 ctgacctatt cccgcccggc cgccgcgccg ggttctccgc tgcaggggac ggctcgccat
    18241 cacgggcgac accatctgcg ccgggttttc cgccggggcg gagcggccgg gcgcttttga
    18301 gcgccgaaag agtgcaaagc gcagtggaga cggaaagacg ttgggtcttg cccggccggg
    18361 caggtctgca ccccaggcgg gcggaaaagg cccgtccgct ggggaagcag agcgctgcca
    18421 acctgcgctc tgagcctgtg tcgtttgcag ccacgccaaa gcggcagacg ggccttttcc
    18481 atcagaatcg ggcgcgctgc atccctccac ctcgccgggg cccgcgcccc tttggtccgc
    18541 cgcgcgggac gggctctgcc accggtggcc gcggggagac tctgacatct cgccggacac
    18601 gccgatctcc agcccgcctt tcgataccca cgggggcggc ccctgcccgc cgggaggctt
    18661 ttcagcgttt tatttcccgg gaaataaggc gtgtttcctt ctctttccgc tctgccccct
    18721 attttatcgc ctccgccccc ccagggcgca ggcggcgggc aggcgtccga cgggagggag
    18781 gaagcgggcc agacgaggga taggtgcccg gcaaaagaga gggagcggaa aggtcggaag
    18841 cgggacaagc atccgacagg agagaagcag caggactggt gggaacaggc acccctgaca
    18901 agaaagcagg gagcgggact gacgcccgac gggagagagc gcgataggtg gggacagaca
    18961 ccctgcggga cagccgggag cgggaaagct gagtgcggaa aagccgggag ggagacggcg
    19021 ggcgggggag ggccgggagc gagacggcgg gctgcccgca aagcagccga cccgaaaaac
    19081 gggaaaaggc ctggtcgcag ggaaagggat agtcggcgga gagggggtag ccgggggaga
    19141 gagggcagcc ggcaaagagg ggcagccggg gcgcggagcc ggccgcagag ccccgcagcc
    19201 gcccggacac acaaagggag ggcggcccga cagccgccct cccttattcc cgattccctt
    19261 tgatgccacg cttttaaaaa tcaccgcggc cgggtgcggg gaccgcatcc ccgttttaaa
    19321 ctctctcgat caccgcggcc gtcaccgcgc tggcggtgcg gcagtaatcg ctgatgtcga
    19381 actgctgttc caccagcttt tcccgcgcgg cctcgtcgca gttgtcgctc atggtgcgca
    19441 ggatgacgaa aggcagatcg tttttcgccg ccacctgcgc caccgcggcg ccctccatct
    19501 ccacacaggc ggggtggcag gcggcctcga tgcggcgctt ggtctcgccg tcgccgatga
    19561 acaggtcgcc ggtggcaacc cggcccaccg tgtagcgcac cccctgcgcg cggcaggcgg
    19621 cctcggccgc cgccaccagg gcgggggagg ccgggtattc ggtgaggaac gggtagctct
    19681 gggcgatcat cggcaactcg gcgtcatggt agcaggccac gtcgctgatg accatatcgc
    19741 ccacccccac gcgggtcgtc atattgcccg caatgccgga aaagagtagc gccttggccc
    19801 cgaacaccgt gatcagcagc tgtgccgccg ccgccgcgtt caccttgccc atgcccgcgc
    19861 agcagacggt gacctggcgg cccgccatgc ggccggtgtg aaactccacc ccggccagcg
    19921 ttttcgcctg cggctcctcc agccgctgcg tcagcaaggc cacctcctcg ggcatcgcgc
    19981 ccagcacgcc gatattgtcg atcataggac acctcttttt actctttttt gtagtatttt
    20041 ttctttatat atataaaata gagcttttcg tgcggcaggg cgactgctct gcgctctttc
    20101 cctgcctttt ataccgcgaa aagggcgctg cccggcaacg cccctttcgt tttttcccct
    20161 tattcaccct tatacattga agcggaacag ggtcacgtcg ccgtcctgca tgacgtactc
    20221 cttgccctcg ctgcgcacca ggcccttttc cttggcggcg gccatgctgc cgcaggtcat
    20281 caggtcgtcg taggccacga tctcggcccg gatgaagccg cgctcaaaat ccgagtggat
    20341 cttgcccgcc gcctgggggg ccttggtgcc ctttttgatg gtccaggccc gcacctccgg
    20401 ctcgcccgcg gtgagaaagg agatcagccc cagcagcgca tagctctcgc ggatcaggtt
    20461 gtccaggccg ctggcctcca ggcccagctc ggccaaaaac tcttttttct cggcgggggt
    20521 gaggccggcg atatcctcct ctgttttggc gcagatgggc atgcagcccg cgccctccgc
    20581 cgcggcgatg tcgcggatca ccgggtaata ggggttctcg ccgatcccgg ccagcaggtc
    20641 gtcctcggac aggttggccg catagatgat cggcttggcg ctgagcaggt gcaggtcgtc
    20701 gaacagtgct ttttcaccgt cctcctccac ctccagcgtg cgggcgtttt tgccgtcgga
    20761 cagatgggcc agcagccgct cgagaaacgc cgcgtccgcc gccagcgcct tgtcgccgcc
    20821 tttcatggcc tttttcgtct tttccagacg gcgctcggtg atctcgatgt cggacaaaat
    20881 cagctcggtg tcgatggtct cgatgtcccg cgccgggtcc acgctgccct ccacgtggat
    20941 gacgtcggtg gaatcaaagc tgcgcaccac gtggatgatg gcgtccacct cgcggatatg
    21001 gctgaggaat ttgttgccca gcccctcgcc cttggacgcg ccctttacca gcccggcgat
    21061 gtccacgaac tcgatggtgg cgggggtgta ttttttgggg tggtacatct cggccagccg
    21121 gtccagccgg gcgtccggca cggccaccac ccccacgttg ggctcgatgg tgcagaacgg
    21181 atagttggcg gactgtgcgc ccgcgttggt gatggcgttg aacagggtgc tcttgcccac
    21241 gttgggcagg ccgacgatgc cgagcttcat gattctattc atcctttcac accgggagac
    21301 gagcccccgg cccggcgcgg gacgggcatc caagcccccg agcgcgccgg tcttttctct
    21361 attgcccaca aagcgcccgg ccttttcgcc ggggcgcttt tttattatag aagaaaacgg
    21421 cgcctctttc aagcgcgggg gctgtttctg ctttttttgt tcacaatcct gcggtataat
    21481 gaaagcatct gtaacgcatt ttcaggccgg gtgcgcccgg tgacgatttt tagggggaac
    21541 aaggatggac aagacgaaga tcctgatcgt ggatgacgac cggaacatct gcgagctgct
    21601 gcggctgtat ctggaaaaag agggctatga gacccagctg gcctacgacg gcgaacaggc
    21661 gctggagcgc tttgctgccg acaagccggg cctgatcctg ctggacgtga tgatgcccaa
    21721 gatcgacggc tgggaggtct gccgccgcat ccgggcctcc tcgcaggtgc cggtgatcat
    21781 gctcacggcc aagggcgaga cctttgacaa ggtgctgggg ctggagctgg gcgccgacga
    21841 ctacatcgtc aagcccttcg acaccaaaga ggtggtggcc cgcatcaagg cggtgctgcg
    21901 ccggtatgcg gcgggcggcg aaaaggagga ggacgcccaa aagctgatct cctacgacaa
    21961 gctcagcgtc gacctgaccc gctatgagct gaaagtaaac ggcaaaaccg tggacgcgcc
    22021 ccccaaagag ctggaactgc tctattatct cgccagccac cccaaccggg tgttcaaccg
    22081 cgaccagctg ctggacgagg tgtggggctt tgaatactac ggcgactcgc gcaccatcga
    22141 cgtacatgtc aagcgcctgc gcgaaaagct ggagggcgtg tccgaccagt ggagcctgaa
    22201 gaccgtgtgg ggcgtgggct ataaatttga ggtcaaggac gaggcctgag gccgcgggcg
    22261 gcgcccggcg aaaggacgat ggtgagcatg aataaaagca tcagcggccg gtatttccgc
    22321 accacggcgc tgctgctggt gtccggcatc acgctgctgg gcttgatttt gatgtatttt
    22381 tgcgtggggt atttccgcac cgagagcgag aacaccctgc tgaccaacgt gcgcagcctg
    22441 cgggacaatt tttccaccgt ctcgatggag gacgccgacc agatgaaaga gcacccctac
    22501 gtggaacagg cggtgcgggt ggtggccaac gccaccggca gcatcttttt cgtgaccgac
    22561 aactacggca agatcctcta ctgctctgac agctccggcg cggtggacac ctcgcgcgcg
    22621 gtgagctcca agatcctcaa ccgcgccttt tccgacgggg agtacgccga gctgggcgag
    22681 ctgtccggca tctacagcca cagctattac accgcgggct gccccctcat caacgacagc
    22741 agccgcatca tcggctatgt gttcgcctcg cgggacgccg cctcgctgac cagcttcctt
    22801 gagagcatct tctcggcgtt catcatctcg gcggccgtga tgctgctggt ctccagcatc
    22861 ctttccatcg tgctcaccaa ccggctggtc aacccgctgc gccgcatcac cgcggccgcc
    22921 cagacctttg gcaacggcga cctgtccgcc cgcgccccgg tgggcggcga cgacgaggtg
    22981 gcgcagctgg ccgtcacttt caacgacatg gcggacaagc tggaggcggt ggacgcctcg
    23041 cgccagagct ttatgggcaa cattgcccac gagctgcgca cccccatgac caccattaag
    23101 ggctttgtgg acggcatgct ggacggcacc atccccgacg accggcgcga ccactatctg
    23161 cagattgtct cgcaggaggt gggccgcctg acccgtctga cccacagcat gctggacatc
    23221 accaagctgg agaccggcga atatgtggtg agcgccgtcg actacgacgt gtgggagacg
    23281 gtgagcagca tcctgttcgc ccgcgaacag cagttcatcg agcacgccat ccgcctgacc
    23341 ggcttcaacc ccgtaaaggt gctggtacac gccgaccccg acctggtgca tcaggtgctg
    23401 tacaacattg tggacaacgc cctgaaattc acccccgacg gcggcaccat cgacatctcg
    23461 gtgaccgaaa acccgaagca gggctttgtg acggtggcga tccgcaacac cggcgagggc
    23521 attcccgccg acgcgctgcc ctatgtcttt gagcgctttt acaaggcgga caagagccgc
    23581 ggcctgcacg cccagggcag cgggctgggc ctgcatatct gcaaacagct gatccaccgc
    23641 tcggacggcc gcatctgggc cgagagcacg ccggggcagg acgccacctt tttgttcacg
    23701 ctgccctgcg gcaacccaaa gccccctgag cgctcgaaat acgcccgcgg cggccagggc
    23761 ggcaaatgag acggtgtgcc ccgccggcaa aaaatcgcgg cgtgccattg cgcctcgtgc
    23821 tcccaatgca gccagccgcc ctttccggtt cgacgcagct ttgccgggcc gctgccgcgc
    23881 ccctcgcacc cgggcggcaa aaaaagcaaa aatatgggcg ggacgcctgc gggcgccccg
    23941 cttttttgtg cgcggctggg gacctgcttt tgggtaccgg ggatcattct ctttcgcatc
    24001 cgccgcgccc attcttccgc tgtcccttta tttttttctt ttatttttaa cttgttttcc
    24061 tttttagatt gttccgttgt ttttctggtc tcctatcggg gaataaatgc cgctatttgt
    24121 ttggtgtccc catcatccat tttttatcaa gtcaattagc ttatttttta tctcaggctc
    24181 agtattcata aaacgcttaa acttatttga ctaagttcta tttttatgat attctgatcc
    24241 ttagaattga ggtgtgcaaa aatgacctta ggggaacgga tcaaaacgat attggcggag
    24301 tgcggcttaa agcaggtgga ctttgccgcc gccctgggcg taagcgccaa ctatgtcaat
    24361 cagctggtca acggcaaaaa agagactctg tccggcactc tggcaaagct catagaagag
    24421 ctgtacggct acccggccca gtgggtcttg acaggggagg gggagaaagc gcccgaagcc
    24481 tccttttccg cggcaaaggc ggagctgctc aaaaaggtct ccaaaatgcc ccaggacgaa
    24541 atcttggccc ttttcgcttt tgccgaatcg ctggaaagcg taaaaaagcg ttttccccca
    24601 cagaatcctt aagcttgtcc ggcgcggcct ggccgcgctt tttttgcgct tccgcccgcc
    24661 cttgcggcca aacgtctaaa agccctgggc cgcaagcccg agaggggaca gtctgtttct
    24721 ccccacgacc ccgatgcctg ccctcttttg gattttcttt tactccccga tgaagacgcc
    24781 gttcctgccg cccgactccc tttcccacgc ccgccgcagg ctctcttttc ccggggaaag
    24841 ggccgctttc acgccc
//
LOCUS       DBMDYA010000002        16256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1074_length_16256_cov_1.542371, whole genome shotgun sequence.
ACCESSION   DBMDYA010000002 DBMDYA010000000
VERSION     DBMDYA010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_16256_cov_1.542371"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="ACZPFK_00105"
     CDS             <1..774
                     /locus_tag="ACZPFK_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1404742.1"
                     /translation="IKYAPEYPEIDFISFDMSATADISGIDNLFGIAFKQNEGSFLAG
                     ALAGKLTKTNKIGAFVFNDVPVGNDFVTGYIAGIREANPDAQVSIAYGGGTADASKLQ
                     EVSSAMFDSGVDVIYGVSGSAYPGLAKEATGRGGIDSGLWCIGVDSDMWTVYKASENA
                     DYADVIVTSMKKNVADSIVYTIDRMVEGSLPFGKVEPYGITEGGVGLADNEYYRSKTP
                     AEVLEYMDGLQKDVVEGKVKVPSYFDFEDYDAFVEWRDQ"
     gene            865..2415
                     /locus_tag="ACZPFK_00110"
     CDS             865..2415
                     /locus_tag="ACZPFK_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404743.1"
                     /translation="MAEVLRMQDITKIYPNGFMANKDVNLAINEGEIHALVGENGAGK
                     TTLMKILFGMEDFQGGRVLLDGKDACIASPLDAIAKGIGMVHQHFMQVPSLTVAENVT
                     LGIEPGQGLLFDKKKAAQITQEISDRYQLKVNANERIADMGVGLRQKVEILKALVRKC
                     RILILDEPTAVLTPQETQELFVQLKALKKDGIAIIFISHKLEEVMALCDRVTVLRAGR
                     TVGADVIENLNPAKISRMMVGRDVVLEIEKEPATPTDIVLQTQDIHYKNAENVMVVNG
                     VSFSIRRGEVVGIAGVEGNGQNEVAELISGMLPLSQGEIRICGESIRGRSVRGIRDMG
                     MAYISEDRMVYGCAANLPIRENIMADRIHSKKYRRFGLFIDTKKINQDIDALIETFEV
                     ACDDRAQPVRMLSGGNIQKVIVAREFSSGAHLILANQPTRGIDVGTTEMIRKTLVRKA
                     REEGVAVLLVSSDLNEVLEVSDRLLVMKDGRIVAHFEDASAVSEELLGEYMLGVRQMA
                     PEEMGDMV"
     gene            2412..3494
                     /locus_tag="ACZPFK_00115"
     CDS             2412..3494
                     /locus_tag="ACZPFK_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404744.1"
                     /translation="MKETRKIEFGFEIFRIVVALAIAYGITLLCIMLISDDPFQAVYM
                     FAVGPFTSIRRLGQLFGKFIPYLLTGAGMCFVYASNRFNLIGEGVYLMSGCLVAYVAV
                     QLGGLGIPHVLFVLLLLVVGALVGALFGSVPALLREKMNINETVVSIMMNWAMLYLAT
                     YLVKTKMYDNSITYMASKPIPQEARLTALIPRTQLHTGLFVGILAVVVTALIFYRTAL
                     GYDIRICGANPKFAKASGINMTATLILAQVMGAALAGLGGAVDILGIYDRYMWTALTN
                     MGFDGLIVAVLSKKNPVFIPLGAFLLAYMRTGASILNYTTEIPIEFVDIMQAVIILLI
                     AAEHFLGGFKEKLIFKASKEKNREVA"
     gene            3497..4414
                     /locus_tag="ACZPFK_00120"
     CDS             3497..4414
                     /locus_tag="ACZPFK_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404745.1"
                     /translation="MLDTIVKFLFSVIRVSTPLIFGAVAACVTRKAGLLNLAIESMML
                     ASALTGVLVSGATQSLALGMLAGFAAAIAITLIISYSAFVLKCDLYLVSIAMNTALVG
                     GTVFVMYLFTQQKANTAGSIPSLFVGNLELPLIRDIPVLGGILSGHNVLTYAAFLMVF
                     VVWFLLYKTKIGLRIRSVGENPQAAESVGISPRRIYFLSFGLSGFIAALGGMYMSMGY
                     LTWFARDMIAGRGMIAMSAMNIAAGEPVSATLAAMLFGTADALSNYMQLGKFPSELIS
                     MFPYVCTVAILVLMAVIRTIKATAARNRI"
     gene            4437..5423
                     /locus_tag="ACZPFK_00125"
     CDS             4437..5423
                     /locus_tag="ACZPFK_00125"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1404746.1"
                     /translation="MKRKVILDVDTGSDDAVAIMSAILSPDLQLEAVCSVAGNKDIDK
                     TTENTLRVVQLLGADVPVYRGCREPLVKFLCPDRLPRRGESKPVMKDGKEVQMHADYL
                     DIPPATIREQPVSAPEFYVRYLRAAKEPVTIVAVGPLTNVAMALMLDGSILDKIEEIV
                     VMGGGYYITNASPSAEFNIWYDPEAAQRVFHCGAKVTIVPLDATHAACITLDDCARFR
                     ALGTVSGDFAAALCEQRITVHDASQPLAVPHAAAVHDALCVVYLIDPAVLRDVRHVHC
                     DVGFRDFCEGQTILDPRYYTEEKNCYFAFDGDRFKFVNVLCDLFARDKKTGR"
     gene            5428..6408
                     /locus_tag="ACZPFK_00130"
     CDS             5428..6408
                     /locus_tag="ACZPFK_00130"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1404747.1"
                     /translation="MATKVILDVDTGSDDAVAIMAAALSPDIELVAICTTWGNQAIED
                     TTDNTLRLADAMGLDVPVYRGCDTAMVKYLTNDYVENRPHVPVMHNGKPVIMHAKHLD
                     LPEATTRPQSLPAACFYVDYLRRTDEKITLIPVGPLTNLGLALRIAPDIAEHIEQIVV
                     MGGGSKITNCNPWSESNIWHDPEAAQIVAECGAKVVWVPLDATHRACITMDDCRRFRE
                     IGTFSANFAAEQCEQRIIVHNAQQPLDVPDAAAVHDALCVAYVIDPTVLTDLRHCHVE
                     IGLAGYGEGQTIVDQRAYPLKKNGYFAYDADRFKFVDILCDCFARDPLKA"
     gene            6685..7616
                     /locus_tag="ACZPFK_00135"
                     /pseudo
     CDS             6685..7616
                     /locus_tag="ACZPFK_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            7613..8605
                     /locus_tag="ACZPFK_00140"
     CDS             7613..8605
                     /locus_tag="ACZPFK_00140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1404748.1"
                     /translation="MNTKKRRIALEMDTGNGGVAAALLAASSGRLGLEGVMACWGALP
                     PADAAAQALMAAELLKKQFPIYPGCPAPLVRDLYRRGVEREESGREKARSVSPGPPLL
                     PDARQGRHAVDYLISRCRSGEGKLTIAVLGPLTDLAAAICIAPDLVKNVREIVVAAGG
                     VGAPPDAFCPQGNVFQDPEAAKIVLESGARVVLFPRTAVCGAALPPGCAARCRAQGGP
                     AAELFARLLEGQALAYSGPQVRRQAGTAAAEAALCIAYLVDSRVVDGLEHARLDICLD
                     RGAGRGVLMRADRRSGKRKNVWIARGAKAELLEAVVLELLRSASTGLEKEEKDV"
     gene            8598..9887
                     /locus_tag="ACZPFK_00145"
     CDS             8598..9887
                     /locus_tag="ACZPFK_00145"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1404749.1"
                     /translation="MFDLVIRNGILVTSAGRREVDIAVQGGRIAALEKGITGPAARVL
                     DAAGLYLFPGLIDAHVHFRDPGFTRKEDFATGSRAALYGGMTYVVDMPNVNPVTSTAQ
                     RLQARMERARREACLEMGFFALLTSDNLEEMAELKQAGAVGFKIYLGTSVGEIAAPAD
                     GVMLEQFRRAARLGMRIGFHAENNAINDYYTALERSAAHPDPGALIRARPDFSEVEAV
                     CKAVAYARETGAAIHIYHVSSGKTVDRIRAAKAEGIDITAETCPHYLLLDETDYERLG
                     VLLKVFPTVKTRWDREKLWAGLADGTIDMIATDHAPHLPEEKQGGLWEAMAGVAGVEI
                     SARLMLDAANRGLLTLEQFAAWMSENPARVWGLKDRGAVRLGGLANLTAVDMEKRGVI
                     HNAKLHGKTNVTAFDGVETTGTPVFSVMGSQLFEIPG"
     gene            10193..10765
                     /gene="rfbC"
                     /locus_tag="ACZPFK_00150"
     CDS             10193..10765
                     /gene="rfbC"
                     /locus_tag="ACZPFK_00150"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1404750.1"
                     /translation="MGKFNFIATELPGVVVIEPTVFPDPRGYFMETYARRDFAQGGID
                     REFVQDNQSKSTRGVLRGLHFQKQHPQGKLVRVISGEVFDVAVDCRPGSPAYGRWTGV
                     TLSAENKKMLYIPEGFAHGFLVLSDEAEFTYKCTDYYTPGDEGGIPWNDPDIGVEWPD
                     AGMAPLTSEKDGRHAGFARQSFEYFKRWAD"
     gene            10765..11571
                     /locus_tag="ACZPFK_00155"
     CDS             10765..11571
                     /locus_tag="ACZPFK_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404751.1"
                     /translation="MEKLKPYFKDFWRYRHLLNNLISRDIKVKYRRSALGLIWSVLNP
                     LLMMLVMYVVFSQIFHMTQMNRGEVEINFAVYLLTGQLMYNFFSEATQTAMSSVLGSA
                     PLIKKVYIPKYIFPLEKVAFSFVNALFSLIALVIVMVFTRTAVHATVLLFWVPMIILF
                     VFNFGVGLILASATVFFRDIMHLYGVLVTALMYLTPIMYDVSILPEWALRFIPFNPMY
                     WFVSMFRDLVIYGQMPGVNAWIGTCASALVALVAGLLLFKKTQDRFVLYI"
     gene            11586..12353
                     /locus_tag="ACZPFK_00160"
     CDS             11586..12353
                     /locus_tag="ACZPFK_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404752.1"
                     /translation="MNIIEVRDVSMRFNMAKERVDNLKEFFIKKAKGTLKFEEFYALK
                     NVSLTVEKGDVYGLVGLNGSGKSTLLKVVAGVLKPTTGTAVVRGAIAPLIELGAGFDM
                     DLTARENIYLNGAVLGLETAFIDSKFDEIVEFSELREFLDVPLKNYSSGMVARIAFAI
                     ATVTKPDVLIADEILSVGDFLFQKKCEQRMKELMGGGTTVLIVSHSIDQIERLCNKVA
                     WLDHGRIKMDGPVEQVCEAYKDLDPDAAAQQESRGKK"
     gene            12350..14215
                     /locus_tag="ACZPFK_00165"
     CDS             12350..14215
                     /locus_tag="ACZPFK_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1404753.1"
                     /translation="MSDSPEKKTAVDERDSVGKAIRRNLSPKKALDLAARGARCLTTR
                     GVEATGREISFRVGLMLGRDDWRHRADIPLRRELRAQRAAKLPRMPLISVVAPLYNTP
                     PLYLRQMVNSVLRQSYQNLELVLVDASDRDHPEVGAFCKSLRDARINYVHLAKNEGIA
                     GNTNLGLAEANGDYLALLDHDDVLQLNALYEMAKAIGETGADFLYSDEIVLGPDLKTL
                     REYHFKPDFSPDTLRGCNYISHFSVFSRALLQSAGGGEDRRYDGAQDFDLILRLTERA
                     GRVCHIPKVLYVWRSHARSTASDISAKPYAIEAGAAAIRAQLDRLGLRGEVEPQEGGP
                     GSYRVRYALTGRPLVSVLIPNRDHVEDLRRCLDSLAAKAGYRELEILVLENNSTEAAT
                     FAFYRDELPNYGNCRLVTYRGPFNFSAINNLGAREAKGEHLLLLNNDIELLSKDFIPE
                     LLMFSQRPDVGAVGAKLYYPDDKIQHAGVFIGLGGSAGHSHKGLPRDNRGDLYRLSTA
                     QNMSAVTGACLMVKASLYRGLGGLDEEAFAVSYNDVDFCLRLREQGYLNVFTPYAEAY
                     HYESKSRGLDTQGPNARRYAGERQRFREKYAALLRDGDPYYNPHFTLLYENYGYK"
     gene            14595..>16256
                     /locus_tag="ACZPFK_00170"
     CDS             14595..>16256
                     /locus_tag="ACZPFK_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1404754.1"
                     /translation="MHRLKRFKELILLFFTMLREDGFRFTFRKMSGFLRRRLRRKKGR
                     FLPARAELERQRREDVSSWPKISVCTALYNTDPKFLKEFLNSFLYQTDQNSELCLADA
                     SDSEHAYVRQIVSACKSARIQYVKLDRNAGISENTNAAARLATGSYLALADHDDILSP
                     NACYELARAAASGAQFIYSDEALFSGDYLRPRVGHFKPDFAPYYLTNCNYICHIAAFP
                     AKLFWQVGGLRPEFDGSQDHDLFFRLTEKTKKICHIPKVLYYWRLHEGSTSGGVGAKP
                     YVAEAAKRAIDEHLARKKVKGRAVDGRFPSTYKVEYDLRVKPLVSILIPNKDHVEDLS
                     RCLESLYGKTTWPKFEVIVIENNSQDEATFRYYEEQQAAHENLKVARYEGAFNFSAIN
                     NFGRSQAQGKFLVLLNNDIEIITPDWVEQMLMLAKQPGVGAVGAMLYYPDDTVQHAGV
                     ITGLGGYAGHSHKYAKRGHSGYMFRLATVQDLSAVTAAMMMVKASVFDEVGGLDEGFT
                     VAFNDVDFCLRLRGAGYSVLFTPYAEAYHCESKSRGQHKKGEAKER"
BASE COUNT         3206 a         4757 c         5228 g         3065 t
ORIGIN      
        1 atcaaatatg caccggaata tcccgagatc gacttcatct cttttgatat gtctgcgacg
       61 gcggatattt cagggatcga caacctgttt gggatcgctt tcaaacagaa cgagggctct
      121 ttcctggccg gcgctctggc gggcaagctg accaagacca acaagatcgg cgcctttgtc
      181 tttaacgacg tgccggtggg caatgatttc gtcaccggct atatcgccgg tatccgcgag
      241 gcaaaccccg atgcacaggt cagcatcgct tatggcggcg gtacggcgga cgcctctaaa
      301 ttgcaggagg tttcttcggc gatgttcgac agcggggtgg atgtcatcta cggtgtttca
      361 gggtcggcct atcccggcct ggcgaaagaa gccaccggcc gcggcggcat cgacagcggg
      421 ctgtggtgca ttggcgtgga cagcgacatg tggacagtct ataaagcgtc tgaaaacgcc
      481 gactatgcgg acgtgattgt cacctccatg aaaaagaatg tggccgactc cattgtatac
      541 accatcgacc gcatggtgga gggctctctt ccgttcggca aggtggagcc ctacggcatc
      601 actgagggcg gcgtcggcct ggcggacaat gagtactatc gctcgaaaac ccctgcagag
      661 gtgctggagt atatggatgg tctgcaaaaa gacgtcgtcg aaggcaaggt aaaagtcccc
      721 tcctattttg atttcgaaga ctacgacgcc tttgtcgagt ggagagatca gtagacggta
      781 agccgcaaag acagttcatt tcatgaagta catggggaga tggacaaagg ttcatctccc
      841 catctggtaa aggggagaaa aaatttggca gaagtacttc gcatgcagga tattaccaag
      901 atctatccca acggttttat ggcgaacaaa gacgtcaatc tggccatcaa cgagggtgag
      961 atccacgctc ttgtgggaga gaacggggcg ggcaagacca cgctgatgaa gatccttttc
     1021 ggcatggagg attttcaggg cggacgggtg ctgctggatg gcaaggacgc ctgtatcgcc
     1081 agccccctgg acgcgatcgc aaagggcatc ggcatggtcc atcagcactt catgcaggtg
     1141 ccctccctga ccgttgcgga aaatgtcacc ctcggcatcg agcccgggca gggcctgctg
     1201 ttcgacaaga aaaaagccgc tcagatcacg caggagatat ccgaccgcta ccaattaaaa
     1261 gtaaacgcca acgagcggat tgcggatatg ggcgtggggc tgcggcagaa agtggagata
     1321 ttaaaagcgc tggtgcgcaa atgccgcatc ctgatcttgg acgagcccac ggcggtgctc
     1381 accccacagg agacccagga gcttttcgta cagctgaaag cgctgaaaaa agacggtatt
     1441 gccatcattt tcatctccca caagctggag gaggtcatgg ctctctgcga ccgggtcacg
     1501 gtgctgcgcg cgggccgcac cgtcggtgcc gatgtgatcg agaatctgaa cccggccaag
     1561 atctcccgca tgatggtggg gcgcgacgtg gtgctggaga ttgaaaaaga gcccgcaacc
     1621 cccacggata tcgtgctgca gacccaggac atccactata aaaacgcgga gaacgtcatg
     1681 gtggtcaacg gcgtgtcctt cagcatccgg cgcggcgagg tcgtggggat cgcgggcgtg
     1741 gagggaaacg gccagaacga ggtggccgag ctcatcagcg gcatgctgcc gctgagccag
     1801 ggcgaaattc gcatctgcgg cgagagcatc cggggcaggt cggtgcgggg cattcgggac
     1861 atgggcatgg cttacatctc agaggaccgc atggtctacg gctgcgccgc aaacctgccc
     1921 atccgggaaa atattatggc ggaccgcatc cactcgaaaa agtatcgccg ctttggcctc
     1981 ttcatcgaca cgaaaaagat caaccaggat attgacgcgc tgatcgagac gtttgaagtg
     2041 gcctgtgacg accgcgccca gccggtgcgg atgctctccg gcggcaatat ccagaaagtc
     2101 attgtggccc gcgagttttc cagcggcgcg catctcatcc tggcaaacca gccgacccgc
     2161 ggcatcgacg tcggcaccac cgagatgatc cgcaagaccc tggtgcgcaa agcgcgggag
     2221 gagggggtgg ccgtgctgct ggtctcctcg gacctgaacg aagtgctgga ggtctccgat
     2281 cgcctgctgg tgatgaaaga cggcaggatc gtcgcacatt ttgaggatgc ctcggcggtg
     2341 agcgaggagc tgctggggga atatatgctg ggcgtcaggc agatggcccc ggaggaaatg
     2401 ggtgatatgg tatgaaagag acaagaaaga ttgagtttgg ctttgagatc ttcaggatcg
     2461 tcgtcgccct tgccatcgcc tacggcatca ccctgctgtg tatcatgctc atcagcgacg
     2521 acccgtttca ggccgtctat atgtttgcgg tggggccgtt cacttccatc cgccggttgg
     2581 ggcagctgtt cggcaaattc atcccctatc tgctgaccgg tgctgggatg tgctttgtct
     2641 acgcctccaa ccggttcaat ctgatcggcg aaggcgtcta cctgatgtcc ggctgtctgg
     2701 tggcgtatgt ggccgtgcag ctgggtgggc tcggcatccc ccacgtcctg ttcgtgctgc
     2761 tgctgctcgt ggtcggcgct cttgtcggcg cgctgttcgg ctcggtgccg gcgctgctgc
     2821 gggaaaagat gaacatcaac gagaccgtgg tctccattat gatgaactgg gcgatgctgt
     2881 atctggccac ctacctggtc aagaccaaaa tgtatgacaa cagcatcacc tacatggcct
     2941 cgaagcccat cccacaggag gcgcgcctga cggcgctcat cccccgcacc cagctgcaca
     3001 ccggcctttt tgtcggcatc ctggcggtgg tggtcaccgc tctgatcttt tacaggacgg
     3061 ccctgggcta tgatatccgc atctgcggcg ccaaccccaa atttgccaag gccagcggca
     3121 tcaacatgac cgccacactg attttggcgc aggtgatggg cgcggcgctg gccggcctcg
     3181 gcggcgccgt ggatatcctg ggcatctacg accgctatat gtggaccgcg ctgaccaaca
     3241 tgggctttga cgggctgatc gttgccgtgc tgtccaaaaa gaacccggtc tttatcccgc
     3301 tgggggcctt tttgctggcc tacatgcgca ccggcgcatc tattttgaac tataccaccg
     3361 agatcccgat cgagtttgtg gacattatgc aggcggtcat cattttgctg attgcggcgg
     3421 agcacttcct cggcgggttc aaggaaaagc tgatctttaa ggcgtcgaaa gaaaagaaca
     3481 gggaggtggc gtgaccgtgc tcgataccat tgtcaagttt ttgttttccg tgatccgcgt
     3541 ctccacaccg ctgatcttcg gcgcggtggc ggcctgcgtc acccgcaagg cgggcctgct
     3601 caacctggcg atcgagagca tgatgctcgc ctcggcgctg accggcgttt tggtcagcgg
     3661 cgcgacccaa agcctcgcgc tgggcatgct ggcggggttt gcggcagcca tcgccatcac
     3721 gctgattatt tcttacagcg cttttgtgct caagtgcgat ctgtatctgg tgagtatcgc
     3781 gatgaacacg gcgctggtgg gcggcaccgt attcgtgatg tatctgttca cccagcagaa
     3841 agccaatacg gccggatcga tccccagcct gttcgtgggc aatctcgaac tgcccctcat
     3901 ccgagatatc ccggttttgg gcggcatcct ttccggccac aacgtgctca cttatgcggc
     3961 gtttttgatg gtgttcgtgg tctggttcct gctctacaaa accaagatcg gcctgcgcat
     4021 ccgctcggtg ggtgaaaatc cccaggccgc cgagtcggtg ggcatctcgc cgcgccgcat
     4081 ctacttttta tccttcggcc tgtctggatt tattgcggcg ctgggcggta tgtatatgtc
     4141 catgggatat ttgacctggt ttgcgcgcga catgattgcg ggccgcggca tgatcgccat
     4201 gtccgccatg aacatcgccg cgggcgagcc ggtcagcgcg acgctggcgg cgatgctgtt
     4261 cggcacggcg gatgcgctct ccaactatat gcagctgggc aagttcccgt ccgagctgat
     4321 ctccatgttc ccctacgtgt gcaccgtcgc cattctggtg ctaatggccg tgatacgcac
     4381 gatcaaagcc acggccgcaa gaaaccgaat ttgaaatgaa cggaggatca atttttatga
     4441 aaagaaaagt aattctcgat gtggacaccg ggtcagacga cgcggtagcc atcatgtctg
     4501 cgatcttgtc gccggatctc cagctggagg ccgtctgctc ggtggcgggg aacaaggaca
     4561 tcgacaagac caccgagaac acgctgcgcg tcgtgcagct gctgggggcc gacgtgccgg
     4621 tttaccgggg ctgccgcgag ccgctggtca aatttttgtg ccccgaccgg ctgccccggc
     4681 gcggcgagtc caagccggtg atgaaagacg gcaaagaggt gcagatgcac gccgattatc
     4741 tggatatccc gcccgcgacc atccgcgaac agccggtcag cgcgccggag ttttatgtcc
     4801 ggtacctgcg cgccgccaaa gaaccggtga ccatcgtcgc cgtgggcccg ctgaccaatg
     4861 tggcgatggc tctgatgctg gacggctcga tcctggataa gatcgaggag atcgttgtga
     4921 tgggcggcgg gtattatatc accaacgcct cccccagtgc cgagtttaat atctggtatg
     4981 acccagaagc cgcgcagcgg gtctttcact gcggcgccaa ggtcacaatc gtccctctgg
     5041 acgccacaca tgccgcttgc atcacgctgg acgactgcgc gcgttttcgc gcgctgggca
     5101 cggtgtcggg cgattttgcg gccgcactgt gcgaacagcg catcacggtg catgacgctt
     5161 cacagccgtt ggcggtgccg cacgcggccg cggtccacga tgcgctctgc gtggtctacc
     5221 tgatcgaccc cgcggtgctg cgggatgttc gccatgtgca ctgcgacgtg ggctttcggg
     5281 atttctgcga gggccagacc atcctggacc cgcgctatta caccgaagag aaaaactgct
     5341 attttgcctt tgacggcgac aggttcaaat ttgtgaatgt cctctgcgac ctgtttgccc
     5401 gcgacaagaa aactgggagg taatgttatg gcgacaaaag tgattctgga cgtcgacact
     5461 ggttcagacg acgccgtggc catcatggcg gcggcgctct ccccggatat cgagctggtg
     5521 gcgatctgca ccacctgggg caaccaggcc attgaggaca ccaccgataa cactttgcgc
     5581 ttggccgatg ccatggggct ggacgtgccg gtctaccggg gctgcgacac ggcgatggtg
     5641 aaatacctga ccaacgacta tgtggaaaac cggccgcatg tgccggtgat gcacaacggc
     5701 aagccggtta tcatgcacgc gaagcacctg gacctgccgg aggcgacgac gcggccccag
     5761 agcctccccg cggcttgctt ttatgtcgat tatctgcggc gcaccgatga aaaaatcacc
     5821 ctgatcccgg tggggccgct caccaatctg ggcctggctc tgcgcatcgc ccccgacatc
     5881 gcagagcata tcgaacagat cgtggtgatg ggcggcggca gcaagattac caactgcaac
     5941 ccctggagcg agtcgaatat ctggcatgac ccggaggcag cccagatcgt ggcggagtgc
     6001 ggcgcaaagg tggtgtgggt gccgctggac gccacccacc gggcctgcat caccatggac
     6061 gattgccggc gctttcggga gatcgggacc ttttccgcca actttgcggc ggagcagtgt
     6121 gagcagcgga tcatcgtcca caacgcccag cagccgctgg atgtccccga tgcggcggcg
     6181 gtgcatgacg ctctgtgcgt ggcctatgtg atcgacccca ccgtgctcac cgatctgcgc
     6241 cactgccatg tggagatcgg cctggcgggc tatggggaag ggcagacgat cgtggatcag
     6301 cgcgcatatc cgctgaaaaa gaacggctat tttgcctatg atgccgaccg attcaagttt
     6361 gtggatattc tgtgcgattg ttttgcccgc gacccgctaa aggcttgaag cgcggctttt
     6421 gtctgtgctg tgccggcggc gcgggcggac aagagcgtcg gccccaagaa aagaaaaaac
     6481 gctgcccgcg ggcaggagca cgctttttag agaaagcggc gccttgcctg ccggcagcat
     6541 gggggccgga cgtcaaatgg tgccgcagcg gcgcgctgcg gcaccatttg gtatccggca
     6601 aaacaggagg cagaagagat gagaaaaatc atcatggatg tggatacggg caccgatgac
     6661 gccattgcga tcatggccgc cgtcatggcg ccccagatcg aggtgaaagc gctgtgcacg
     6721 gtgcacggga ataccagtgt ggaaaacacc acgcgcaaca ccctcaaggc ggcctttgcc
     6781 gccggcggcg cggatatccc cgtctatccc ggcgccgcag cccctatggt aaaaaacttg
     6841 tcggcggtca gggcggcccc cgtgccggag ccggtgctga gcgggacggc gcagatcgac
     6901 ggggtaacgg tctcgatgaa ccccgatgtt ctgcccctgc cggactctcc cagtgccccg
     6961 caggagatgc cggcggcgct gtattatctg gaatttttgc gcagcacacc ggaaaaaatc
     7021 accatcgtcg ccaccggagc cttgacgaac ctgggtcttg cctcacgctg gacccgcgca
     7081 tcgcggacag tatagaagag ctcgtcatca tgggcggggg ggtgaacaaa agcaacatca
     7141 ctgcttccgc agaggcgaat ttttataagg acccggaggc tgcggcgatc gttttgggct
     7201 gtggcgcccc gatcacgctc tgcacgctgg acgccaccca cagctgcgcc ctcactgaag
     7261 cccacgaacg gcgtttgagg gcggtgggca acacggcggc aatcttcacc gccaacgatg
     7321 tgcgcgcccg gcgggagagc tacgcgcgct ttcagccgct ggagcggccg gatacggcgc
     7381 cgatccacga tgcgctttgc attgcctacc tgctcgaccc gggggtgctg ctccgcgtgg
     7441 aggaagcggc gtgcgaggtg gactgctccg acggcatcag cgagggcagg ctgcaggtgg
     7501 acacccgcta ttttcacgcg ccgccgaatg tccgcttggt cacacaggcg gacccggaca
     7561 gaatgtgtga gatcctgctg gaggcctttt ccggaaaagg gaggggagcc ggatgaacac
     7621 aaaaaagaga cgcattgctt tggagatgga cacgggcaac ggcggagttg ccgctgccct
     7681 gctggcagcg tcgtccggga ggctggggtt ggagggggtg atggcgtgct ggggcgccct
     7741 gccgcctgcg gacgccgcag cgcaggcgct catggcggcg gagctgctga aaaagcagtt
     7801 ccccatctac ccgggctgtc ccgcaccgct ggtgcgggac ctctaccggc gcggggtgga
     7861 aagggaggaa agcgggcgcg aaaaggcgcg cagcgtctcc cccgggccgc cgctgctccc
     7921 cgacgccagg cagggaagac atgcggtgga ttatctcatc agcagatgcc gctccggcga
     7981 gggaaaactg acgatcgcgg tgttgggacc gttgaccgac ctggcagccg cgatctgtat
     8041 cgcccctgat ctggtgaaaa atgtccggga gatcgttgtg gcggcgggag gagtgggggc
     8101 gccgcccgac gctttttgcc cacagggcaa tgtctttcag gacccggagg cggccaaaat
     8161 cgtgcttgaa agcggcgcgc gggtagtgct ttttccgcgg acggcagtct gtggggcggc
     8221 gctgccgccg ggctgcgctg cgagatgccg ggcgcagggc gggcccgccg cagaactttt
     8281 tgcccggctg ctggaggggc aggccctcgc ttacagcggt ccgcaggttc gacgacaggc
     8341 ggggacggcc gcggccgaag cggcgctgtg catcgcctat ctggtggact cccgcgtggt
     8401 cgacgggctg gagcatgccc gtctcgacat ctgcctggac cggggggcgg gacggggcgt
     8461 tctgatgcgc gccgaccggc gctccgggaa aaggaaaaac gtctggatag cgcgcggggc
     8521 aaaggcggag ttgcttgagg cggtcgtgct ggagctgctg cgcagcgcgt cgaccggcct
     8581 ggaaaaggag gaaaaagatg tttgatcttg tgatccgcaa cgggattttg gtgacttcgg
     8641 cagggcgccg ggaggtggat attgcggtcc agggcgggcg gatcgccgca ttggaaaaag
     8701 gaatcacggg cccggccgcc cgggtccttg atgcggcggg gctctatctc tttccggggc
     8761 tgatcgatgc gcatgtgcac tttcgggacc cgggctttac ccggaaagag gactttgcca
     8821 ccggctcccg cgcggcgctg tacggcggta tgacctacgt ggtggatatg cccaacgtga
     8881 acccggtcac ctccacggcg cagcgcctgc aggcgcgcat ggagcgcgcc cgcagagagg
     8941 cctgcctgga gatgggcttt tttgccctgc tcaccagcga caacctggag gagatggcgg
     9001 agctgaaaca ggcgggcgct gtgggattta aaatttatct cggcacctcg gtgggggaga
     9061 tcgcggcgcc ggcggacgga gtgatgctgg agcaattccg ccgggccgcc cgcctgggga
     9121 tgcggatcgg cttccacgca gagaacaatg caatcaacga ttattacacc gcgctggagc
     9181 ggtcggcagc ccacccggac cccggcgccc tgatccgggc gcgtccggat ttttctgagg
     9241 tggaggcggt ctgcaaggcc gtcgcctatg ccagggagac cggcgcggcc atccatattt
     9301 accatgtgag cagcggaaaa acagtggata ggatacgggc cgccaaggcg gaggggatcg
     9361 acataaccgc agagacctgc ccacattatc tcctgctcga cgaaacggac tatgaaaggc
     9421 tcggggttct gctgaaagtg tttcccaccg tcaaaactcg atgggacaga gaaaagcttt
     9481 gggcaggcct cgccgatgga acgatcgata tgatcgcgac ggaccatgcc ccgcatcttc
     9541 cggaggaaaa gcaaggcggg ctgtgggagg ccatggccgg ggtggcaggg gtcgagattt
     9601 cagcgcggct gatgctggac gcagccaacc ggggtctgct cacgctggaa cagttcgctg
     9661 cgtggatgag tgagaaccca gcccgcgtct ggggactgaa agaccggggt gcggtgcgcc
     9721 tgggcggatt ggccaacctg accgcagtgg atatggaaaa gcgcggggtg atacacaatg
     9781 cgaagctgca tggaaagacc aatgtgacgg cgtttgacgg ggtggagacg actgggaccc
     9841 ctgtgttttc tgtgatggga agccaattgt ttgagatacc cggataaaga tgcaggaagg
     9901 aaaggcgtag ctcgccgaga ccgcagggaa aaagacggcc tcaaaaagct ttaaggcgga
     9961 caaaaccagc agcccaagag ggggccggag ccggcggtac agccggtccc ggccccctct
    10021 tgcggtgcgg cggggccggg aatcgtttta aaaaagatac aaactgcccc tcaaagtgga
    10081 aatttgcctg cgactatggt agaatacaga atgaattaaa gcgcgcaaaa ctgtttttgc
    10141 cgtgtttttc gctctttcca gggcaaaata aaggtaattt gaggtaaata aaatgggtaa
    10201 attcaacttt atcgccaccg aactgcccgg cgtcgtggtc atcgagccga ccgtcttccc
    10261 cgatccgcgc ggctatttta tggagaccta tgcccgccgc gactttgcgc aggggggcat
    10321 cgaccgcgag tttgtacagg acaaccagtc caaaagcacg cggggcgtgt tgcggggcct
    10381 gcactttcaa aagcagcacc cccagggcaa gctggtgcgg gtgatctcgg gcgaggtctt
    10441 tgatgtggcg gtggactgcc gccccggcag cccggcctac ggcagatgga ccggcgtcac
    10501 cctctcggct gaaaacaaaa aaatgctcta tatccccgag ggctttgccc acggttttct
    10561 ggtgctgtcc gacgaggcgg agttcaccta taaatgcacc gattattaca ccccgggcga
    10621 cgagggcggc atcccctgga acgaccctga tatcggcgtc gagtggccgg acgccggcat
    10681 ggcgccgctg accagcgaaa aggacggcag acatgccggc tttgcccggc agagctttga
    10741 gtactttaaa aggtgggccg actgatggag aagctcaagc cctattttaa agatttttgg
    10801 cgttaccgcc atctgctgaa caacctcatc agccgggaca tcaaggtcaa gtaccgccgc
    10861 agcgcgctgg gcctgatctg gagcgtgctc aacccgctgc tgatgatgct ggtgatgtat
    10921 gtggtcttca gccagatctt ccacatgacc cagatgaacc gcggcgaggt ggagattaac
    10981 tttgccgtct acctgctcac cggccagctg atgtacaact ttttctccga ggcgacccag
    11041 acggccatga gcagcgtgct gggcagcgcg ccgctgatca aaaaggtcta tatccccaaa
    11101 tacatttttc cgctggaaaa ggtggccttt tcctttgtca acgccctgtt cagcctgatt
    11161 gccctggtga tcgtcatggt gttcacccgc accgcggtgc acgccaccgt gctgctgttc
    11221 tgggtgccca tgatcatcct gtttgtcttc aacttcggcg tggggctgat cctggcctcg
    11281 gccacggtgt tcttccgcga catcatgcat ctgtacggcg tgctggtcac ggcgctgatg
    11341 tacctcaccc ccatcatgta cgacgtgagt atcctgcccg aatgggcgct cagatttatc
    11401 ccgttcaacc cgatgtactg gtttgtcagc atgttccgcg acctcgtcat ctatgggcag
    11461 atgccgggcg tcaacgcctg gatcggcacc tgcgcctcgg cgctggtggc gctggtggcg
    11521 ggcctgctgc tcttcaaaaa aacccaggac cgctttgtcc tgtacattta aaggggaatc
    11581 acggtatgaa tatcatcgaa gtgcgcgacg tctcgatgcg ctttaacatg gccaaggagc
    11641 gggtggacaa cctcaaggag tttttcatca aaaaggcgaa gggcaccctg aaatttgagg
    11701 agttctacgc cctcaaaaac gtctcgctga cggtggaaaa gggggacgtc tacggcctgg
    11761 tgggcctcaa cggcagcggc aaatcgacgc tgctcaaggt cgtggccggg gtgctcaagc
    11821 ccaccaccgg caccgccgtg gtgcggggcg ccatcgcgcc gctgatcgag ctgggcgcgg
    11881 gctttgacat ggacctcacc gcgcgggaga acatctacct caacggcgcg gtgctggggc
    11941 tggagacggc gtttatcgac tcgaaattcg acgagatcgt ggagttcagc gagctgcggg
    12001 agtttctcga cgtgccgctg aaaaactact cctccggcat ggtggcccgc atcgctttcg
    12061 ccatcgccac cgtcaccaag ccggatgtgc tcatcgcgga cgagatcctc tcggtgggcg
    12121 actttctgtt ccagaaaaag tgcgagcagc ggatgaaaga gctgatgggc gggggcacca
    12181 cggtgctcat tgtcagccat tccatcgacc agatcgagcg gctgtgcaac aaggtggcct
    12241 ggctggatca cggccgcatc aagatggacg gcccggtgga gcaggtctgc gaggcctata
    12301 aggacctgga ccccgacgcg gcggcgcagc aggaatcgag agggaagaaa tgagcgattc
    12361 acctgaaaaa aagaccgctg tggacgagcg cgacagcgtg ggcaaggcca ttcggcgcaa
    12421 cctttccccc aaaaaggcgc tggacctggc cgcccgcggc gcgcggtgcc tcaccacccg
    12481 gggcgtggag gccaccgggc gggagatctc tttccgggtg gggctgatgc tgggccggga
    12541 cgactggcgt caccgggcgg acatccccct gcggcgggag ctgcgggcgc agcgggcggc
    12601 caagctgccc cgcatgccgc tgatcagcgt ggtggcgccg ctgtacaata cgccgccgct
    12661 gtacctgcgc cagatggtga acagcgtgct gcgccagagc tatcaaaatc tggagctggt
    12721 gctggtggac gccagcgacc gggatcatcc cgaggtgggc gcgttctgca aatccctgcg
    12781 ggacgcgcgg atcaactatg tccacctggc aaaaaacgag ggcatcgccg gcaacaccaa
    12841 cctggggctt gccgaggcga acggcgacta cctggccctg ctggaccacg acgacgtgct
    12901 gcagctgaac gccctctatg agatggcaaa ggccatcggc gagacagggg cggattttct
    12961 ctacagcgat gagatcgtgc tggggccgga cctgaagacc ctgcgcgaat accatttcaa
    13021 gccggatttt tcccccgaca cgctgcgggg ctgcaattac atcagccatt tttcggtctt
    13081 cagccgcgcg ctgctgcagt cggcgggcgg cggcgaggac cggcgctatg acggcgcgca
    13141 ggattttgac ctgatcctgc ggctgaccga gcgcgccggg cgggtctgcc atatccccaa
    13201 ggtgctctat gtctggcgca gccacgcccg ctccaccgcc agcgatatct cggcaaagcc
    13261 ctacgccatc gaggcggggg ccgccgccat ccgggcccag ctggaccggc tgggcctgcg
    13321 gggcgaggtg gagccccagg agggcggccc gggcagctac cgggtgcgct atgccctcac
    13381 gggccgcccg ctggtctcgg tgctcatccc aaaccgggac catgtggagg acctgcgccg
    13441 ctgcctggac agcctggccg cgaaagcggg ctaccgcgag ctggagatcc tggtgctgga
    13501 gaacaactcc accgaggccg cgacctttgc gttttacagg gacgagctgc cgaactacgg
    13561 caactgccgg ctggtgacct accgcgggcc gttcaacttt tcagcgatca acaacctggg
    13621 cgcgcgggag gcgaaagggg agcatctgct gctgctcaac aacgacatcg agctgctctc
    13681 aaaggatttt atccccgagc tgctgatgtt ctcccagcgg ccggacgtgg gcgcggtggg
    13741 ggcaaagctg tattatccgg acgacaagat ccagcacgcc ggggtcttca tcggtcttgg
    13801 cggcagcgcc gggcacagcc acaagggctt gccgcgggac aaccggggcg acctgtaccg
    13861 cctctccacc gcccagaaca tgagcgcggt gaccggggcc tgcctgatgg tgaaagcctc
    13921 gctctaccgc gggctgggcg gcctggacga ggaggcgttc gcggtcagct acaacgacgt
    13981 ggacttctgc ctgcggctgc gggagcaggg ctatctgaac gttttcaccc cctatgccga
    14041 ggcgtatcac tatgagagca agagccgcgg cctggacacc caggggccca acgcccggcg
    14101 ttatgcgggc gaacgccagc gctttcgcga aaaatacgcg gcgctgctgc gagacggcga
    14161 cccctactac aacccccatt ttacgctttt gtatgagaac tacggctata aataagggag
    14221 aggcggcctg ccgcggtttt tagggctctt gaatggttcg ccccgccgcg gcgggagagg
    14281 gagagtccgg atcgcgaggt agaaactgct tttttggctt cgcccctcag ttttgccgct
    14341 gcggcggcgc gaaaaaaagg ggcctgcggc cgcgcggacc cggacaggct ctttgcctct
    14401 gccgccgtaa gagggcgccc ggtacaggag ccgtgcgcgc gggccgcgac ggcggtctgc
    14461 gcctgaatcc accaccccaa tccgcccggc cgcgaggttg ggaaaatagc cgcgcctgcg
    14521 ctttgcgtcc gcgctgggcg agtgggccga atcgtggcca gaccgccccg attttatccc
    14581 tgtaaggaga acatatgcat cgtctcaaac ggtttaagga actgatattg ctgtttttca
    14641 caatgctgcg ggaggacggt ttccgcttca ctttccgcaa gatgagcggg tttctgcgcc
    14701 gcaggctgcg ccgcaaaaag ggccgctttc tgcccgcccg ggcggagctg gagcgccagc
    14761 ggcgggagga cgtctcctcc tggcccaaga tcagcgtctg caccgcgctc tacaacacgg
    14821 accccaagtt tctcaaagag tttttgaaca gctttctcta ccagaccgac cagaactcgg
    14881 agctctgtct cgccgacgcc agcgattcgg agcacgcgta tgtgcggcag attgtctcgg
    14941 cctgcaagag cgcgcgcatc cagtatgtga agctggaccg caacgcgggc atcagcgaga
    15001 acaccaatgc cgccgcgcgg ctggccaccg gcagttatct ggccctggcg gaccacgacg
    15061 acatcctctc gcccaacgcc tgctacgagc tggcgcgggc ggccgcctcg ggcgcccagt
    15121 tcatctacag cgacgaggcc ctgttttccg gcgactatct gcgtccccgg gtgggccact
    15181 tcaagccgga tttcgcaccc tattatctga ccaactgcaa ctatatctgc catatcgcgg
    15241 ctttcccggc gaagctgttt tggcaggtgg gcggcctgcg gcccgagttt gacggcagcc
    15301 aggaccacga cctctttttc cgcctcaccg aaaagaccaa aaagatctgc cacatcccca
    15361 aggtgctcta ttactggcgg ctgcacgagg gcagcaccag cggcggcgtg ggggccaagc
    15421 cctatgtggc cgaggctgca aagcgggcca tcgacgagca cctggcccgc aaaaaggtca
    15481 agggacgcgc ggtggacggc cgttttccct ccacctacaa agtggagtat gacctgcggg
    15541 tgaagccgct ggtctcgatt ttgatcccga acaaggatca cgtggaggac ctctcccgct
    15601 gcctggagag cctttacggc aagacgacct ggcccaaatt cgaggtcatc gtcatcgaga
    15661 acaattccca ggacgaggcc actttccgct attatgagga gcagcaggcc gcgcatgaaa
    15721 acctgaaagt ggcccgctat gagggcgcgt tcaacttttc agccatcaac aacttcggcc
    15781 gcagccaggc gcagggcaag tttctggtgc tgctcaacaa cgacatcgag atcatcacgc
    15841 cggactgggt cgagcagatg ctgatgctgg caaaacagcc gggtgtgggc gcggtgggcg
    15901 ccatgctcta ttacccggac gacaccgtgc agcacgccgg ggtgatcacg ggtcttggcg
    15961 gctatgccgg gcacagccac aaatatgcaa agcgcggcca cagcggctat atgttccgcc
    16021 tggcgaccgt gcaggacctc tcggcggtga cggcggccat gatgatggtc aaggcctctg
    16081 tctttgacga ggtgggcggc ctggacgagg gctttaccgt ggcgttcaac gacgtggatt
    16141 tctgcctgcg gctgcgcggg gcgggctaca gcgtcctctt caccccctat gccgaggcct
    16201 accactgcga gagcaaaagc aggggccagc acaagaaagg cgaggcaaag gaacgc
//
LOCUS       DBMDYA010000003        13648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1310_length_13648_cov_1.608696, whole genome shotgun sequence.
ACCESSION   DBMDYA010000003 DBMDYA010000000
VERSION     DBMDYA010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_13648_cov_1.608696"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            328..1476
                     /locus_tag="ACZPFK_00175"
     CDS             328..1476
                     /locus_tag="ACZPFK_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1404755.1"
                     /translation="MVTQDIRYIGVDDKTLDLFEGQYPIPNGIAYNSYLILDDKVAVM
                     DTVDKRKTAEYLDNLERELAGRSPDYLVVTHVEPDHASSIRALVDRYPGIRLVGNAKT
                     FQMLPQYFDLDLSGAVTVKEGDTLSLGRHELSFLMAPMVHWPEVMVAYDSCDKVLFSA
                     DAFGKFGALDAEEDWACEARRYYFNIVGKYGPQVQALLKKAAALDIRAICPLHGPVLK
                     EDLGYYLGKYDLWSSYGVEQEGVFIAYASLHGNTAGAATHLAELLRGKGCPSVAVADL
                     ARADLSEALEDAFKYGKVVLAASSYNAGVMPFMEDFLHHLKAKNYQKRTVALIENGSW
                     APSAARTMKELLAQMKEIQLVEPTITLRGAVKQGDLEALEALADTLLR"
     gene            2086..3468
                     /locus_tag="ACZPFK_00180"
     CDS             2086..3468
                     /locus_tag="ACZPFK_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1404756.1"
                     /translation="MFEAHDVTVGTPWKRIAELTVPMLIGNIAQQLYNTVDTIVVGRY
                     VGDNALAAVGGASPILNLLLVLFVGISTGASILVSQRFGARDRDGLARVIGNCMTVTA
                     IATVLSMAVGLVAIRPLLTAMNTPPSIYEWCADYLNIFFWGIVGFVFYNILSGILRGL
                     GDNVSALGFLLISTVINIGLDLWFVAGFGMGVAGVALATVIAQGISAVLCALKLVNMR
                     QHFDLKPSDLRLDGSCVKKIIGLGLPSGVSQGVFSISALLVQSLTNSLGEMVMACNVV
                     VMRVDGFAMMPAFSVGVAMTTYAGQNYGAGKLDRLRSGARQGMAVAFFMSLVLTLGIL
                     FFGGPLASIFTDTQALIELSQRMLRILSLGYLIFGVNQGLSGFMRGTGDTTTPMWIGI
                     ITQVVLRLPLAYLLAWLTRGGEYPNGRPEALFVSLVISWVIATVLTYFAYRRRVARMQ
                     TELATGHAQG"
     gene            complement(3672..4880)
                     /locus_tag="ACZPFK_00185"
     CDS             complement(3672..4880)
                     /locus_tag="ACZPFK_00185"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1404757.1"
                     /translation="MKYDFTSILDRHGRDALAVDGLGHGGFAPAAPKEGFDAIPMWVA
                     DMNFPTVPTVPEALIARANHPAYGYFTPTDAYYDSIIRWHQQRNGVTGLTRDCIGYEN
                     GVLGGVVSALNVFCSRGDNVLLHSPTYIGFTSCVENNGYNIVHTPLVQDEDGVWRMDY
                     EDMDRKLKQYKIHAAIFCSPHNPCGRVWERWEIEKAMEVYRANEVMVVSDEIWSDIIL
                     PGHRHIPTQSVSEDARMRTVAVYAPSKTFNLAGLVGSYHIIYNPYLRERIAKESSLSH
                     YNDMNVLSMHALIGAYRPEGYEWVDELCQVLGGNVDYACDYIAAHFKGVRVSKPQGTY
                     MLFLDCSEWCAAHGKTIDDVEKAGWEVGVAWQDGRMFHGPCALRVNLALPLSRVKEAF
                     ARMDKYVFNA"
     gene            complement(5223..6395)
                     /locus_tag="ACZPFK_00190"
     CDS             complement(5223..6395)
                     /locus_tag="ACZPFK_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013237.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR1404758.1"
                     /translation="MSRYDTDFSEMDFATRAIKSGEGPDPVTRALNTPIYETTTYGYE
                     STEEYDRMLAEGADWAPGVYIYSRTTNPTTVAMEKKVASLENAEDACICACGMAAVSN
                     ALFSTLNAGDHVICSDDTFLCTSSMFADILPSKGIETSRVEILDLANIENAMRPNTKV
                     VYLEVLSNPQLKLANIPAIAELAHAHGCKLIVDNTFLSPYVLRPLDLGADIVVQSGTK
                     YYVGHGDALCGIVAGRKEDIDRVRYYNDNLGTHISTFDAWLALRGTRTLALRVARQSE
                     NALALAKWFEARPEVEYVIYPGLESHKQHELANKLFHGGHYGGMLCVHLKGGYEEMAK
                     FADSTRIPPIATSLGDVVTLMFPKKPYNNLIRLSVGCEDVNDLIADFEQAFNKMKG"
     gene            complement(7424..7500)
                     /locus_tag="ACZPFK_00195"
     tRNA            complement(7424..7500)
                     /locus_tag="ACZPFK_00195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7464..7466),aa:Met,seq:cat)
     gene            complement(7633..8787)
                     /locus_tag="ACZPFK_00200"
     CDS             complement(7633..8787)
                     /locus_tag="ACZPFK_00200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1404759.1"
                     /translation="MDSKQLAAQVQEYVVAFRHELHEHPELSMEEVWTTDRIVKELES
                     MGLTVHRFEPTGAMADIKGGKPGKTVALRADIDALPVEEHTGLPFASKVDGVMHACGH
                     DTHAAMLMGAAKALVSVKDEIPGTVRLIFQPAEETVQGAKALVKQGALEGVDMIFGQH
                     IAAMMPVGAVATVAGPSAAASDHFGIKVHGKGCHGAMPNTGVDATLAAANVVTALQSI
                     VSREIAPAEPAVVTVGRFVSGTKNNIVSGEAEMAGTVRTFDRELRKSMPERIERIAKE
                     IAAAYRCTADVEYEFAVDVLINDQHATDITLGALKKVAADPRMILTNLPRQMGAEDFA
                     EYTPVVPGSFAMLGGGGEYPQHSDKFCIDERCFETGVAFLIQVAFDALNA"
     gene            9007..9603
                     /locus_tag="ACZPFK_00205"
     CDS             9007..9603
                     /locus_tag="ACZPFK_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1404760.1"
                     /translation="MNDFLQAMQARRSYYALSKASPVGDERLEALVRQAVAHTPSAFN
                     MQSGRAVLLLGMHHDKLWQITLEALAAVTPPERFEATRQKIAAFAAGHGTVLFFDDTA
                     VTQSFARQNPLYSASFPVWAQQAGGMLQFAVWTLLEEAGLGASLQHYNPLIDEAVKRV
                     WQIPDSWQLLAQMPFGAPAGAPGEKAFEPLERRVKVYR"
     gene            9706..9957
                     /locus_tag="ACZPFK_00210"
     CDS             9706..9957
                     /locus_tag="ACZPFK_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404761.1"
                     /translation="MIAFWNRAELLTTTSMRQQAEARVALAAAGIDYSCKTSGLMSQG
                     GRGHAGSFGINMDCAYTYAIYVRKKDLEQAQYVVWRKGR"
     gene            10181..10897
                     /locus_tag="ACZPFK_00215"
     CDS             10181..10897
                     /locus_tag="ACZPFK_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR1404762.1"
                     /translation="MRGKLNSFTAGGRQVFVYAPPGAERPLPLVQMAAGEELAAQLPQ
                     VMGEVEPLLGRGCAPFLLAWTAPVDWERDYTPWPAPALPGRPPFAGGADAYLDLLAGT
                     VRPAVAARFDILSGPGDTALAGYSLGGLLALCGLLSRPEYGRAASLSGSLWYDGWAAW
                     QAEHPARPGCRCYLSLGKAEERSRDARMSRVGRATRAASELLRGQLGPQNAVLEWNNG
                     GHFTGVPHRIAKGLLWLMRG"
     gene            11007..11630
                     /locus_tag="ACZPFK_00220"
     CDS             11007..11630
                     /locus_tag="ACZPFK_00220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR1404763.1"
                     /translation="MCCRYYIDQSLMRGLFDLFHGLPQVEQQRLSELSGEVFPTGRAP
                     AVRAAGEEAALFVPRWGFAGQDRRQPVVNARAETVAERPMFRESFALRRCLVPASRFF
                     EWDKLTSQGRGPKTKYAFTRADGAPLLLAGCWKRGPEGERFVVLTTAANSSMAEIHDR
                     MPVLIAPENARDYLFDEASARLLATQQGPELLREAQLPPGGGEQLRF"
     gene            11649..12329
                     /locus_tag="ACZPFK_00225"
     CDS             11649..12329
                     /locus_tag="ACZPFK_00225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1404764.1"
                     /translation="MTAETYALLEGYMLRCMGDSAHDKEHVYRVLYTALDIAGSEREA
                     DREVLISACLLHDIGRREQFEDPRLDHAAVGAEKAQRFLTAHGFEEGMARRVAACIRT
                     HRFRADRPPESLEARILFDADKLEATGALGIARTLLYTGRVGQPLYSLTPQGRVSDGA
                     GEEAPSFFHEYCCKLEGLYGRFFTRRGEELARLRRQAAADFYQALLAETRLPYERGPA
                     LLRAALDG"
     gene            12421..13557
                     /locus_tag="ACZPFK_00230"
     CDS             12421..13557
                     /locus_tag="ACZPFK_00230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1404765.1"
                     /translation="MELYFEPGREADLDELGRLYDRLNEHLEQTVNYPGWRKGVYPVR
                     ETAAEGVAAHSLFVAKSEGRIAGALILWRRQDPAYRKAAWVVNAREEEVLTVHTLAVH
                     PAFGGRGVGEALIRFAIRYACRAGAKALRLDVCEGNRPAIRLYERCGFTQVGTVDLGL
                     HDWGLDWFRLYEKAVPPPAECPWGCSWEPERDDGERLFLAPPAPLWREAVLAYQEEHL
                     AAGEKKLSGASLLGELGFDEWMASARAGSGPACPQGGRLVPLLALRKSDGALVGMAEF
                     CLRSGGSLAAEGGQISCGVRPSQRGRGYGSALLRLAIEKAASQGLPGAKVVCGRDNEP
                     ARRIILRCGGVLEREFVHTDGRTMQVYGLGLTGADGGPGARARG"
BASE COUNT         2418 a         4048 c         4716 g         2466 t
ORIGIN      
        1 tgtggcgcga gggcaaagcg gagcggcaaa agccgcgccc tctgcctcga ttttaccata
       61 cggccccgca aatggaaaga agcggaaacg gcccgtcggc cgctgagggc ggggtacgcg
      121 gcggcggaga aaaaagagac gccgcctgcg ccgcggcccc ctatggtgcc ccgtgaggcc
      181 gccgccggca agcccgcgtc cgcaaaaaaa tcgcggcaaa aagtgacaac gcgggtcgga
      241 ttgtgtataa tcttcttgtg ctatgaaatc gtgtgggcgc gcccgcgcgg cgccgcctgc
      301 gcagatcaca ttttcggagg tgtccgtatg gttacgcaag acattcgcta tatcggtgtg
      361 gacgacaaaa cgctggacct cttcgagggc cagtatccca tccccaacgg gatcgcctac
      421 aattcctatc tcatcctgga cgacaaggtc gcggtgatgg acaccgtgga caagcgcaaa
      481 acggcggaat acctggacaa cctggagcgg gagctggcgg gccgcagccc ggactacctg
      541 gtggtcaccc atgtggagcc ggaccacgcc tccagtatcc gggcgctggt ggaccggtac
      601 ccgggcatcc ggctggtggg caacgccaaa acctttcaga tgctgccgca gtatttcgac
      661 ctggacctga gcggcgcggt caccgtcaag gagggggaca ccctgtccct ggggcgtcac
      721 gagctcagct tcctgatggc ccccatggtg cattggcccg aggtgatggt ggcctatgac
      781 agctgcgaca aggtgctgtt cagcgccgac gcgttcggta aattcggcgc gctggacgcc
      841 gaggaggact gggcctgcga ggcgcgccgg tactatttca acattgtggg caagtacggc
      901 ccccaggtac aggcgctgct gaaaaaagcg gctgcgctgg acatccgcgc gatctgcccg
      961 ctgcacgggc cggtgctgaa agaggacctg ggctattacc tggggaaata tgacctgtgg
     1021 agcagctacg gcgtggagca ggagggcgtc tttatcgcct atgcctcgct gcacggcaac
     1081 accgccggcg cggccaccca cctggcggag ctgctgcgcg gcaagggctg ccccagcgtg
     1141 gcggtcgccg acctggcccg cgccgatctc tcagaggccc tggaggacgc gttcaagtac
     1201 ggcaaggtgg tgctggcggc gtcctcgtac aacgccgggg tcatgccctt tatggaggat
     1261 ttcctccacc acttgaaagc gaagaactat caaaaacgca ccgtggccct gatcgaaaac
     1321 ggctcctggg cgccctcggc cgcgcggacg atgaaagagc tcctcgccca gatgaaagag
     1381 atccagctgg tcgagcccac catcaccctc cgcggcgcgg taaagcaggg cgacctggag
     1441 gcgctggagg cgctggcgga cacgctgctg cgctgattgc ccgctttgct gcggggccct
     1501 gtccaccggg acagggccct tttttgtatc ccaggcccgg tgcgggcggc caatggaaga
     1561 gggaactgcc gcttttagag ggcacacggg aaacgtgcgg ttatggcggg gagcgattgg
     1621 ttttggcgcg cagggcaagg ctgagcggcg ccgatggttt gcaaagacgg cccggcgcgg
     1681 ggaggcggag cgccgcgggc gcggtgaact tgcacgcgcg tccaaggcgg cagacgggct
     1741 ttttttgtcg gaagcaggga atcgccgttt ccggtcaaag ctgcgcagaa cagcggcggc
     1801 ccgcctgttt ttcatcgggc cggggcgcgc gggcctttcc ttggggcagc cggcgcagac
     1861 gaaagctcaa gcgccggccg ggaaacagcg gacgcgggac agccggggcc tgcgcccggg
     1921 cgcagggcgg tattcttcgc ccccctgcag gggcgcggcg cgcaagttgc cattgcggaa
     1981 caggggaaaa tcctttatac tgtttttata tggggatgct ctaatcaggg gcatccccct
     2041 gattaggaca taagggaaag ggcgaggaga ttgggagttg caaaattgtt cgaggcgcac
     2101 gacgtcacgg tgggcacacc atggaaacgc atcgccgagc tgacggtccc catgctgatc
     2161 ggcaacatcg cgcagcagct ctacaacacg gtggacacca tcgtggtggg gcggtatgtg
     2221 ggagacaatg cgctggctgc ggtgggcggc gcctctccca tcctcaatct gctgctggtg
     2281 ctctttgtgg gcatctccac cggcgcgagc attctggtct cgcagcggtt cggggcgcgg
     2341 gacagggacg gtcttgcccg ggtcatcggc aactgcatga cggtgaccgc catcgcgacc
     2401 gtgctcagca tggccgtggg cctggtggcc atccgcccgc tgctcaccgc catgaacacg
     2461 ccgcccagca tctacgagtg gtgtgcggat tatctcaaca tcttcttttg ggggatcgtg
     2521 ggctttgtct tttacaacat cctctccggc atcctgcgcg ggctggggga caatgtctcg
     2581 gctctgggct ttttgctgat ctccaccgtc atcaacatcg gcctggacct gtggttcgtg
     2641 gcggggttcg gcatgggggt ggcgggcgtg gccctggcca cggtgatcgc ccagggcatc
     2701 tccgcggtgc tctgcgccct caagctggtc aacatgcgcc agcactttga cctcaagccc
     2761 agcgacctgc gcctggacgg cagctgtgtg aaaaagatca tcggtctggg tctgcccagc
     2821 ggcgtctccc agggcgtgtt ctccatctcg gcgctgctgg tgcagtcgct caccaacagc
     2881 ctgggcgaga tggtgatggc ctgcaacgtg gtggtgatgc gggtggacgg cttcgccatg
     2941 atgcccgctt tttcggtggg ggtggcgatg accacctatg cgggccagaa ttacggcgcg
     3001 ggcaagctgg acaggctccg ctccggcgcg cgccagggca tggcggtggc gttttttatg
     3061 tctctggtgc tgacgctggg catcctgttc ttcggcggcc cgctggcctc gatcttcacc
     3121 gacacgcagg cgctcatcga gctcagccag cgcatgctgc gcatcctctc gctggggtat
     3181 ctgatctttg gcgtcaacca ggggctgagc ggctttatgc gcggcaccgg tgataccacg
     3241 acccccatgt ggatcggcat catcacccag gtggtgctgc ggctgccgct ggcctatctg
     3301 ctggcctggc tcacccgggg cggcgagtac cccaacggca ggccggaggc gctgtttgta
     3361 tcgctggtga tctcctgggt gatcgccacg gtgctgacct attttgccta ccgccgccgg
     3421 gtggcccgca tgcagacgga gcttgccacg ggccacgcgc aggggtgacc cacccggctg
     3481 ctgcggcctc aaaacggccc ctgacggggc ctgcctgagg gtgtagtgaa aatcaaaaaa
     3541 cgaaaagcgc gcgggccggc ccaaaaaaga gggccggccc gcgcgctttt ttgattgttt
     3601 tttccgcctg cccgccgcgc cgcatcgccc gttgcccgcg gggagcgaaa ggccgggcag
     3661 cccgccggca ttcaggcgtt gaagacgtat ttgtccatgc gcgcgaaagc ctctttcacg
     3721 cgggagaggg gcagggccag gttgacccgc agcgcacagg ggccgtggaa catccgcccg
     3781 tcctgccagg cgacgcccac ctcccagccg gccttttcca cgtcgtcgat ggttttgccg
     3841 tgggcggcgc accactcgct gcagtccaaa aagagcatgt aagtgccctg cggctttgag
     3901 acccgcaccc ccttgaaatg cgcggcgata tagtcgcagg cgtagtccac gttgcccccc
     3961 aatacctggc agagctcgtc cacccactcg tacccctcgg ggcggtaggc gccgatcagc
     4021 gcgtgcatcg acagcacgtt catgtcgttg tagtgggaca gggaggattc ctttgcaatc
     4081 cgctcgcgca gataggggtt gtagatgatg tggtagctgc ccaccaggcc ggccagattg
     4141 aaagtcttgg agggcgcgta gacggccacg gtgcgcatgc gcgcatcctc ggataccgac
     4201 tgggtgggga tgtgccgatg gccgggcagg atgatgtcgg accagatctc gtccgagacc
     4261 accatcacct cgttggcccg gtagacctcc atggcctttt cgatctccca gcgctcccac
     4321 acgcgcccgc aggggttgtg gggagagcag aagatggccg cgtggatttt gtactgcttg
     4381 agcttgcggt ccatgtcctc gtagtccatg cgccagacgc catcctcatc ctgcaccagc
     4441 ggggtgtgga cgatgttgta gccgttgttc tccacacagc tggtgaagcc gatgtaggtg
     4501 ggcgagtgca gcaggacatt gtccccccgg gagcaaaaga cgttcagcgc gctcaccacg
     4561 ccgcccagca cgccgttttc atagccgatg cagtcgcggg tgaggccggt gacgccgttg
     4621 cgctgctgat gccagcggat gatggaatca taataggcgt cggtgggggt gaaatagccg
     4681 taagcggggt gattggcccg cgcgatgagg gcctcgggca ccgtgggcac ggtgggaaag
     4741 ttcatgtccg cgacccacat ggggatggcg tcgaagccct ctttcggcgc ggcgggcgca
     4801 aagccgccgt ggcccaggcc gtccacagcg agggcatccc tgccgtggcg gtcgaggatg
     4861 ctggtgaaat catatttcat gagacccgtc ctttctgtct gcaagagcaa aatgagatca
     4921 ctttcattat accgcctttg ccgggggcgg acaagcgcaa aagcggggcc ggggagagac
     4981 ggggagcggg gagagcgcaa actgcccgaa aggaatcgtg tttgcggccg ccggtgagag
     5041 gcaaataaaa aacgccccct ctgggagggg gcgttccaaa atacagccgc ggcaggcgag
     5101 ttttcttaca aggcatccgg gccgggccga taagggcccg tgggacccgg agcgtcctca
     5161 ccgctctgcg gggcgaaagg gctggtgcgg ggcctgtatc cgtcgcgagg cccggataga
     5221 gctcaccctt tcatcttgtt gaaagcctgc tcgaagtcgg cgatcaggtc gttcacgtcc
     5281 tcgcagccga ccgacagccg gatcaggttg ttgtagggct ttttggggaa catgagggtg
     5341 accacgtcgc ccaggctcgt cgcgatgggc gggatgcggg tgctgtcggc aaacttcgcc
     5401 atctcctcat agccgccttt caggtgcacg cagagcatgc cgccgtagtg gccgccgtgg
     5461 aagagcttgt tcgccagctc atgctgtttg tggctctcga gacccggata gatgacatac
     5521 tccacctccg ggcgcgcctc aaaccatttt gccagggcca gagcgttttc agactgacgg
     5581 gcgacgcgca gcgccagcgt acgggtgccg cgcagggcga gccaggcgtc gaacgtgctg
     5641 atgtgggtgc ccaggttgtc gttgtaatag cgcacccggt cgatgtcctc tttgcggccc
     5701 gcgacaatgc cgcagagggc gtcgccatgg cccacatagt atttggtgcc ggattggacc
     5761 acgatgtccg cgcccagatc cagcggacgc aggacatagg gggagaggaa cgtgttgtcc
     5821 acgatgagct tgcagccgtg ggcgtgcgcc agctcggcga tggcggggat gttggccagc
     5881 ttcagctgcg ggttggacag aacctcaaga tagaccactt tcgtgttcgg ccgcattgcg
     5941 ttttcgatgt tcgccaggtc gagaatctcc acgcgggagg tctcgatgcc cttggagggc
     6001 aggatatccg cgaacatgct cgaggtgcac aggaacgtgt cgtccgagca gatgacgtgg
     6061 tcgcccgcgt tcagcgtgga aaagagggcg ttgctcaccg ccgccatgcc gcaggcacag
     6121 atgcaggcgt cctcggcgtt ttcaagcgac gcgacctttt tctccatcgc cacggtggtg
     6181 gggttggtgg tgcggctgta gatgtagacg cccggcgccc agtcggcgcc ctcggcgagc
     6241 atccgatcgt attcctccgt ggattcatag ccgtaggtgg tggtctcgta gatgggcgtg
     6301 ttcaaagcgc gggtcaccgg atccggcccc tcgccgctct tgatcgcgcg ggtggcaaaa
     6361 tccatttcgc tgaaatcagt atcgtatctg ctcatataca tcctccctgt ctgacaataa
     6421 aatgtttgtc cgcttttgct agagccatta taccccatat tccgccgttt cgcaaacata
     6481 tttcgcgcgg ccccggccgc gcgaaaagac agaaaaacgc gccggccgcc gcctgctgaa
     6541 aaacctgtcg cggcgaagcg gaataaaaaa taaaggcttt ccgcccggcg cagggggctt
     6601 ttgcttttac gtctcgagga ggtttaaaag tatcggtgca ggcggacggc cgactggggg
     6661 cacaaaaaag aaaaaagtct caaaaataga tgcgcatgtt gcgaaaaaat taatttacgg
     6721 ttgatgcaga atcgtcgaca aaaagttcgt aaaataagcg aatttccttg tctcttttta
     6781 ttatgttact atgccttttc gatattttct cagtttatcc atctttttaa aactatccat
     6841 tcaggaaata tcacacattc catccgaaca agttgttgaa actttaacac tgtagaacaa
     6901 aaagaaagga aatcgagtga aataatgaca tttattggct gatcctcaga ataatcttct
     6961 ccttctaaaa caggaaaata caagaagcca ttttgctgtt gtgcggatac aataccagat
     7021 tcagttgctc gtatggccga ggccgaccaa actctcccat actcggtaaa cggattatag
     7081 gctactcgaa tacaaggggt tccgttggga tcagtttttg ttctggacgc agtaatattc
     7141 agattgatcg aatcaattga caaagtagtt gggatctgtg aaggattcca attgggaatc
     7201 agatcagagg taacttgttc atagtatcct tcatactcaa tacctataat ttcaaccggc
     7261 tctgagacaa atggcgccga aatgaaagat aaaggacttt tggagcatta gaatttgaga
     7321 agagagatca gaaagagaaa gaacataaga cgaaaagatt ttttataata cctcaaaaag
     7381 atatgaaaaa aaccgtgtaa acattatgtt tacacggttt aaatggttgc ggaggcagga
     7441 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccggactgct ccactccgcg
     7501 atatccggcc ctattcttta gggctagatt ataataccac gaagtggaac gggtgtcaag
     7561 agcaaatttt gaaaaaatga tatccggcct gaaaacagcc tttttgcccg ccctgatcgg
     7621 ccgggcggcg gctcatgcgt tcagcgcgtc gaacgccacc tggatgagaa aagcgacgcc
     7681 cgtctcaaag cagcgctcgt cgatgcagaa cttgtcgctg tgctgcgggt actcgccgcc
     7741 gccgcccagc atcgcaaacg agcccggcac caccggggtg tactcggcaa agtcctcggc
     7801 gcccatctgg cggggcaggt tggtgaggat catgcgcggg tcggcggcca cctttttcag
     7861 cgcgcccagc gtgatgtcgg tggcgtgctg gtcgttgatc agcacgtcca cggcaaactc
     7921 gtactccacg tcggcggtac agcggtaggc ggcggcgatc tccttggcga tgcgctcgat
     7981 gcgctcgggc atgctcttgc gcagctcacg gtcaaaagtg cgcacggtgc cggccatctc
     8041 ggcctcgccg gagacgatgt tgtttttggt gcccgagaca aagcggccca cggtgaccac
     8101 ggcgggctcg gcgggggcga tctcgcggct gacgatgctc tgcagggcgg tgaccacgtt
     8161 ggcggcggcc agggtggcgt ccacgccggt gttgggcatg gcgccgtggc agcccttgcc
     8221 gtgtaccttg atgccgaagt ggtcggaggc ggcggcggac ggcccggcga cggtggccac
     8281 cgcgcccacc ggcatcatgg cggcgatgtg ctggccgaag atcatgtcca cgccctccag
     8341 tgcgccctgc ttcaccaggg ccttggcgcc ctgcacggtc tcctcagcgg gctggaagat
     8401 cagccgcacg gtgccgggga tctcgtcttt cacgctgacc agggccttgg cggcgcccat
     8461 cagcatggcc gcgtgggtgt cgtggccgca ggcgtgcatg acgccgtcca ccttggaggc
     8521 aaagggcagg ccggtgtgct cctccaccgg cagcgcgtcg atgtcggcgc gcagggccac
     8581 ggtcttgccc ggcttgccgc ccttgatgtc cgccatggcg ccggtgggct caaagcggtg
     8641 gacggtcagg cccatggact ccagctcttt gacgatgcgg tcggtggtcc acacctcctc
     8701 catgctcagc tccgggtgct catgcagctc gtggcggaaa gcgaccacat actcctgtac
     8761 ctgtgcggcc aactgtttgc tgtccatatc gcaactactc cttttttgcc ccgccgggcc
     8821 gggtggatcg ggccgcgatg cggggctttt gtttgttacc agtataccgc ggcacgccgc
     8881 ccggcgcaag cccgcggcgc gcagccgccg gttgaaagaa cgggagaaaa ggggtataat
     8941 aaaattatac cgtttaagta taaatcgaaa aattacacac aatcatcacg agggaggtca
     9001 aaagggatga acgatttttt acaggccatg caggccaggc gcagctatta tgcgctctca
     9061 aaggcgtcgc cggtgggcga cgagaggctg gaggcgctgg tgcgccaggc ggtggcgcac
     9121 acgccctccg ctttcaatat gcagagcggg cgcgcggtgc tgctgctggg catgcatcac
     9181 gacaagctgt ggcagatcac gctggaagcg ctggccgccg tcacgccgcc ggagcggttt
     9241 gaggccacaa ggcaaaagat cgccgccttt gccgcgggcc acggcacggt gttgtttttt
     9301 gacgacacgg cggtgaccca gagctttgca cggcagaacc cgctctacag cgccagcttc
     9361 ccggtttggg cccagcaggc ggggggcatg ctgcagttcg cggtctggac cctgctggag
     9421 gaggcgggcc tgggggcgtc gctgcagcac tataatccgc tcatcgacga ggcggtcaag
     9481 cgggtgtggc agatccccga cagctggcag ctgttggccc agatgccctt tggcgcccct
     9541 gcgggcgcgc cgggtgaaaa ggcgttcgag ccgctggagc ggcgggtgaa agtttaccgc
     9601 tgaaccgccg tgccgcggcg gccgatctgt gttatactga gagcagagag actgggggcg
     9661 gggcgtgcgg acatccctgc ttcccggcac aggggggcgg cgtttgtgat cgctttttgg
     9721 aacagggccg agctgctgac gacgacttcg atgcggcagc aggcggaggc gcgggtggcg
     9781 ctggccgccg cgggcatcga ctacagctgc aagaccagcg gcttgatgtc ccagggcggc
     9841 cgggggcacg caggcagctt tggtatcaat atggactgcg cctataccta tgcgatctat
     9901 gtgcgcaaaa aagatctgga gcaggcgcag tatgtggtct ggcgcaaagg gcgctgaccg
     9961 ctgcgcgccc cgagcgtccg ctggccgccg cagccggtga gaacgcataa ctgcggcggg
    10021 gccgcgcgat ggcgcccccc gccgcggcga tgaaaacagc gctgcggggc agcgcctgtc
    10081 ttgccggctt tgcggcgttg ccgctgccgg cggaaatgac ccggggcacg ggcggcaacg
    10141 gccgcccaag acggctgatc gaagcgggga gagcaggtcc atgagaggaa aattgaacag
    10201 cttcaccgcg ggcggccgcc aggtttttgt ctacgcgccg ccgggcgcag agcggccgct
    10261 gccgctggtg cagatggcgg cgggagaaga gctagcggca cagctgccgc aggtcatggg
    10321 cgaggtggag ccgctgctgg ggcgcggctg cgcccccttt ttgctggcct ggaccgcgcc
    10381 ggtggactgg gagcgggact ataccccctg gcccgcgccc gccctgccgg ggcggccgcc
    10441 ctttgccggg ggcgcggacg catatctcga tctgctggcg ggcacagtaa gaccggcggt
    10501 ggcggcgcgg ttcgacattc tgtccgggcc gggcgacacg gcgctggcgg gatattcgct
    10561 gggcggtctg ctggcgttgt gcgggctgct ctcccggccc gaatacgggc gggcggccag
    10621 cctttccggc tcgctgtggt acgacggctg ggccgcctgg caggcggagc acccggcgcg
    10681 gccggggtgc cgctgctatc tctcgctggg caaggcggag gagcgcagcc gggatgcgcg
    10741 gatgtcccgc gtgggccggg ccacccgcgc cgcgtccgag ctgctgcggg ggcagctcgg
    10801 gccgcagaac gcggtgctgg aatggaacaa cggcggccac ttcaccggcg ttccccaccg
    10861 catcgccaag ggcttgctct ggctgatgcg cggctgagcg cccggggcgg cccggctggg
    10921 ctccttttcc ggcggatcaa gccgccggct gacgcgcggc tggcacccgg agagggacaa
    10981 gatcaaaacg gaggaggcgg ggcggtttgt gctgccggta ttatattgat caaagcttga
    11041 tgcggggcct gttcgacctg ttccacgggc tgccgcaggt ggagcagcag cggctgtccg
    11101 agctgtcggg ggaggtcttt cccaccgggc gcgcgccggc ggtgcgcgcc gcgggggagg
    11161 aggcggcgct gttcgtgccc cgctggggct ttgccgggca ggaccggcgg cagccggtgg
    11221 tgaacgcccg ggccgagacg gtggcggagc ggccgatgtt ccgcgagagc tttgccctgc
    11281 gccgctgtct ggtgcccgcc agccgctttt ttgagtggga caagctcacc tcccagggac
    11341 gggggccaaa gacaaaatat gctttcaccc gtgccgacgg cgcgccgctg ctgctggccg
    11401 gctgctggaa gcgcggcccc gagggcgagc gctttgtggt gctgaccacg gcggccaaca
    11461 gctcgatggc ggagatccat gaccgcatgc cggtgctcat cgcgccggaa aacgcgcggg
    11521 attacctgtt tgacgaggcg tcggcccggc tgctggcgac ccagcagggc ccggagctgc
    11581 tgcgggaggc gcagctgcct ccgggcgggg gcgaacagct gcgcttttga gcgggagggg
    11641 gaaacaggat gacggcagag acctatgcgc tgctggaggg ctatatgctc cgctgcatgg
    11701 gggacagcgc ccacgacaag gagcacgttt accgcgtgct gtacacggcg ctggacatcg
    11761 ccggcagcga gcgggaggcg gaccgggagg tgctgatcag cgcctgcctg ctgcacgata
    11821 tcgggcggcg ggagcagttt gaagacccgc gcctcgacca cgccgccgtt ggggcggaaa
    11881 aagcgcagcg ctttttgacg gcgcacggct ttgaggaggg gatggcccgg cgggtggcag
    11941 cgtgcattcg cacgcaccgc ttccgtgccg accggccgcc cgagagcctg gaggcaagga
    12001 tcctcttcga cgccgacaag ctggaggcga ccggggcgct gggcatcgcg cgcacgcttc
    12061 tctacaccgg ccgggtgggg cagccgctct actctctgac gccccagggg cgggtgtccg
    12121 acggcgcggg ggaggaggcc ccctcctttt ttcacgagta ctgctgcaag ctggaggggc
    12181 tgtacggccg gttttttacc cggcgcgggg aagagctggc ccgtctgcgc cgacaggcgg
    12241 cggcggactt ttatcaggcg ctgctggccg agacccgcct gccctatgag cggggcccgg
    12301 cgctgctgcg cgccgcgctg gacgggtgag cggccgggac ggggagatac ataaaacgac
    12361 gcggcaagag gcgcgtttgt cccccgggcc cgcagaggag agcaaagggg agacacgcgg
    12421 ttggaattgt attttgaacc gggccgggaa gccgacctgg acgagttggg gcggctttac
    12481 gaccgcttga acgaacatct ggaacagacc gtgaactatc cgggctggag gaaaggcgtc
    12541 tatcccgtgc gggagaccgc ggccgagggg gtggcggcgc acagcctttt tgtggcgaaa
    12601 agcgagggcc gtatcgccgg cgccctcatc ctctggcgcc gccaggatcc cgcttaccga
    12661 aaagccgcct gggtggtgaa cgcgcgggag gaagaggtgc tcaccgtcca caccctggcg
    12721 gtgcaccccg cctttggcgg gcgcggggtg ggcgaggcgc tgatccggtt tgcgatccgc
    12781 tacgcctgcc gtgccggggc caaggccctg cgcctggacg tctgcgaggg caaccggccc
    12841 gcgatccggc tctatgagag gtgcgggttc acccaggtgg gcaccgtgga tctggggctg
    12901 cacgactggg ggctggactg gttccgtcta tatgaaaaag cggtgccgcc gccggcggaa
    12961 tgcccctggg gctgcagctg ggagccggag cgggacgatg gggagaggct gtttctcgcg
    13021 cctcccgccc cgctgtggcg ggaggcggtg ctggcctatc aagaggaaca cctcgcggcg
    13081 ggggagaaga agctgagcgg cgcttcgctg ttgggcgagc tggggtttga cgagtggatg
    13141 gcgtcggcgc gcgcgggctc cggccccgcg tgcccgcagg gcgggcggct cgtccccctg
    13201 ctcgccctgc gcaaaagcga cggcgctctg gtgggcatgg ccgagttctg cctccggtcc
    13261 ggcggctctc tggctgccga gggcgggcag atcagctgcg gcgtgcggcc ctcgcagcgg
    13321 ggcaggggat atggctccgc cctcctgcgg ctggcaatag aaaaggcggc ctcccagggg
    13381 ctgcccgggg ccaaggtggt ctgcggccgg gacaacgagc ccgcgcggcg gataatcctg
    13441 cgctgcggcg gtgtgctgga gcgggaattt gtacatacgg acggcaggac gatgcaggtc
    13501 tatgggctcg gcttgacagg ggcggacggc gggcccggcg cgcgggcgcg gggctgacgg
    13561 ccgccgggca cggccgcacg gtcacacggc ggcagggcgg ggcaacgcgg cccgccgcct
    13621 tgagatacac gctcaaaaat ggaaagga
//
LOCUS       DBMDYA010000004        13362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1341_length_13362_cov_1.849106, whole genome shotgun sequence.
ACCESSION   DBMDYA010000004 DBMDYA010000000
VERSION     DBMDYA010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_13362_cov_1.849106"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="ACZPFK_00235"
     CDS             <1..305
                     /locus_tag="ACZPFK_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein C-terminal
                     domain-containing protein"
                     /protein_id="HIR1404766.1"
                     /translation="ARALALDPKIILLDEPAAGMNPNETNSLREFILQLNADGYTIVV
                     IEHDMKFVMNSCNRILVLNFGQKICEGTPEEVRGNREVNEAYFGKGKMLKGGGHSA"
     gene            298..1008
                     /locus_tag="ACZPFK_00240"
     CDS             298..1008
                     /locus_tag="ACZPFK_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404767.1"
                     /translation="MLEVRDLSVSYGYITALAGASFEVREGEIVTLIGSNGAGKTTSL
                     MAISNLVDKEEGSVTFKGQDISRMPPAKIVTMGMSHVPEGRKIFPSLTVYENLIAGTL
                     GRPGMTRAQVQRQLDTVYSLFPRLKDRAGQGGGTLSGGEQQMLAIARGLMMDPDILML
                     DEPSLGLAPILVEEIFELLEEIHRTGKTILLIEQNAAMALSIADRGYVLENGRVALSG
                     TGEELLHNDEVMRVYLGG"
     gene            complement(1088..1720)
                     /locus_tag="ACZPFK_00245"
     CDS             complement(1088..1720)
                     /locus_tag="ACZPFK_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1404768.1"
                     /translation="MAATLKQQIYNAIWEGIVNGEYRADHVFNEKTLVEKYQVSKSPV
                     RDALIELCSDGVLRSIPRYGYEIVQISNKQLQEIIRLRALIEVDNLEMMVGRISPEQI
                     AQIKAHILETDRKLENGTLTVRNHWENNIAFHKMLYSMSNNQFGASLLDRCLKAETMA
                     YSMYCLYTVEQNDLLDSKGHWAIVNCMEQSDREGAAAALRKDLESISLAL"
     gene            1851..2828
                     /locus_tag="ACZPFK_00250"
     CDS             1851..2828
                     /locus_tag="ACZPFK_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR1404769.1"
                     /translation="MQKKVGKKVNKRERVLAAYRGQKPDRVPCAFWYHFPAGYEYGEK
                     AVQIHLDFFESSGTDLCKVMNENSCPDDPAIRTAADWSGLKPFTMEEPFVRRQLELVR
                     RVSQRVDGQAVVLATVHGLVASAYHILGGPDLYDADGTVLGRHLRENPEGMRHAFRLI
                     TDYLKALCRGCLEAGADGIYFASLGGERRMFTDEEFAAFIAPYEIEVLESIRDVPCFN
                     VLHMCKSDLNLERYLGYPAQVLNWGVYEENISLQEGRALFGQNKVYLGGLDDRAGVIV
                     EGSLEEIGAEVRRLIGEFGWHNFILGADCTLPTGLPAERMQAAARAAAL"
     gene            2849..4027
                     /locus_tag="ACZPFK_00255"
     CDS             2849..4027
                     /locus_tag="ACZPFK_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1404770.1"
                     /translation="MGSLMNWNFGYYVPTEVVFGCGRVAEVGELARRFGTRALLVTVP
                     EFEAVHGLYEKVKTSLRDAGVEVFHFDGVVPNPTTDVVSAGARTAKQEKVQVVIGLGG
                     GSSMDTAKAIAVEASHEGTAWDYLYYKTPPTDKTLPIIAISTTAGTGSQATSVAVVTN
                     SAKKDKSALSNCNLFPRIAVVDPEVTATMPASVTAQTGFDAFCHNFESYLSVRASAMV
                     EALALDAIRRVIEYLPRAVGDGTDMEARTQMAWVDTLGGFTNACAGTTLPHGLGMQIG
                     GHCPQVTHGQALAAVYPSFTRYTRPAAVGKFAAVARVFDPALAGVDDETASLRCCELI
                     DEFLKKIHLWIGLKGLGVSLQDVREIADCGQVLGDYKRNPRVATIEEMYQILMESYER
                     "
     gene            4046..4468
                     /locus_tag="ACZPFK_00260"
     CDS             4046..4468
                     /locus_tag="ACZPFK_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR1404771.1"
                     /translation="MLKGIPSILPPELLKILMEMGHGDELVIGDGNFPAAALGQRLVR
                     CDGHGVPEVLDAVLTLMPLDFVVEAPVTLMHSTMGTPDPPVWEEYRRILSLREPEHQG
                     IATVERYEFYERAKRAYAVVATGETAMRGNILLKKGVC"
     gene            4673..5020
                     /locus_tag="ACZPFK_00265"
     CDS             4673..5020
                     /locus_tag="ACZPFK_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404772.1"
                     /translation="MPKRRANASYTGEFKQKVIEDMRNNGLSQNETAAKYQVPRSRVQ
                     SWERIYLEEGPEGLYMERRGRGGKGKPTNLDKQVEEDLIAENQRLRAEVAYLKNLQAL
                     VLEDERHQRKKCR"
     gene            5011..5859
                     /locus_tag="ACZPFK_00270"
     CDS             5011..5859
                     /locus_tag="ACZPFK_00270"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1404773.1"
                     /translation="MQVIQKLRQSFPLELLLDIAQLPRATYYYHVKRQAAPDKYLDAQ
                     AAITGIYHENKGRYGYRRITADLRNRGFGLNHKTVQRLMKKLGLVCRVRMKKYHSYKG
                     EVGKIVPNLLERNFEAKKPNQKWVTDVTEFHLFGQKLYLSPILDLCSRDIVSYALSDR
                     PGLSMVIRMLDSAFAQISDSTNLILHSDQGWQYQHKHYQRMLKKKGIRQSMSRKGNCL
                     DNAVIENFFGLLKSELLYLQKFESMQQFKAELIEYLDYYNNSRAKAKLKGLPPAVHRQ
                     QALLAA"
     gene            complement(5923..6426)
                     /locus_tag="ACZPFK_00275"
     CDS             complement(5923..6426)
                     /locus_tag="ACZPFK_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404774.1"
                     /translation="MKKLIVLAVTACLLFTACGGDGSSASGGASSSRPSSSSAAQPTA
                     TPKATAAPTATPQKDASSSSASPSSSEVAQDVSAWVGDYAKQPGSAGQILLTVTEGGE
                     AGLIHFVLDAKGLTVEGDATVYKDGGALCEAQGNLTLSLGRDGVTVTEGKELNPNVAF
                     SGLYEKF"
     gene            complement(6530..7342)
                     /locus_tag="ACZPFK_00280"
     CDS             complement(6530..7342)
                     /locus_tag="ACZPFK_00280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1404775.1"
                     /translation="MQYNHTLPRAVICDVDGTLVDRERKEPTARLLACLERARRQGAL
                     VLVASGRIRQLVPAALRETADYCVCGNGGIVLDRGGDVVCETPYAPELVEELAAFCRR
                     IGGALSFSFPSGYGAYCDFERMRRMYLESTGMDGGITDETARRDRHLREAPFSAFLIG
                     NEGEVYRYLRGQPRLQGARQWQDHFDIYPVATTKAAGISQVLQRHGIDWKDVVAFGDS
                     PNDLEMLAAAGRGYAMGNACPEAKELTAYLAPPVWEDGVAQVLEEIFGSGKK"
     gene            complement(7367..8344)
                     /locus_tag="ACZPFK_00285"
     CDS             complement(7367..8344)
                     /locus_tag="ACZPFK_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1404776.1"
                     /translation="MAKQKFRFANEVRKIIYFLLANGCYAFAIQLFLAGNNIAAGGFS
                     GIAIVLSSFLPVSIGTFVFLLNIPFILLAFFIKGPRYTLLTLCGSTVYALILDAISFL
                     PTVTHNKLVAAVFGGLIYGVGAVLFLKSGASNGGTDLVARLLLIKFRSMSVGKMFLIV
                     DGSVVLFAMLMFRDIEAGLYAIITIYVCGMVNDRILNGFDKANMCYIISEKDPVALSD
                     AIQKEIHRGVTLQRGVGMYQGYERNILMTVVRPRETYHLKEVVQGLDEKAFVVMAEVN
                     EVLGRGFKGFADETPVDAPELPVIPVPAGKKKKKAHKKEKEKKEAKTGV"
     gene            complement(8748..9197)
                     /locus_tag="ACZPFK_00290"
     CDS             complement(8748..9197)
                     /locus_tag="ACZPFK_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1404777.1"
                     /translation="MFGLMPYERRERGMMDLFSDLDKYFFGEKSMPSMPSMPALSCRT
                     DIEDKGDSYLLSAELPGFNKEDIKLDLDGDVLTISAVHSEESEKKEKDGSYVCRERHY
                     GSYSRSFDVSTIRQEEIKAAYKNGVLELTLPKKEAAPAPAARAIEIE"
     gene            complement(9515..10219)
                     /locus_tag="ACZPFK_00295"
     CDS             complement(9515..10219)
                     /locus_tag="ACZPFK_00295"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1404778.1"
                     /translation="MDAKLRELIEGSGNIVFFGGAGVSTESGIPDFRSVDGLYHQKYA
                     YPPETILSHTFFERHTEEFFDFYRDKMLYLDAQPNAAHRMLAELEARGKCRAVVTQNI
                     DGLHQRAGSKHVFELHGSVLRNHCVRCGRAYGVEAVKESRGIPRCSCGGVIKPDVVLY
                     EEGLDGAVLEGAVRAIAACDLLIIGGTSLAVYPAAGLIDYYRGHKLVVINKTPTPADR
                     RADHLFHGAIGEILGR"
     gene            complement(10219..10578)
                     /locus_tag="ACZPFK_00300"
     CDS             complement(10219..10578)
                     /locus_tag="ACZPFK_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404779.1"
                     /translation="MDKKETLWALGLGLALFAGVPALARYLLPPEAGMAFSMLFLLAV
                     HPVFFLAAGAFCGRRPRSRWPLPALLAALFIFGYGLVLRMWEPFYPLLYLALGYLALA
                     AAALRRRVRRGGTRRGS"
     gene            10941..12497
                     /locus_tag="ACZPFK_00305"
     CDS             10941..12497
                     /locus_tag="ACZPFK_00305"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR1404780.1"
                     /translation="MSVKIMTAREAMDLVPDGATVATGGFIGASFAEELALELENRFL
                     ETGHPRDLSLMFCAAQGDRKDKGLNHLAHEGMLKGAMGAHWGLAPKIGQMVADNKILA
                     YVFPQGVMCHMLRDSAAHRPGTLTHVGLGTFVDPRLGGGKINDLTRAAPDRVHLMPLG
                     GKEYLFYDYQKVDFALLCGTYADENGNISMERCAVTGEMLATAQACKNSGGTVVVQVE
                     RVVKAGSLDPKKVEIPGILVDAVVPVSDMKYHMQTFDVEYNPALSGEHRVSLKAAEPL
                     PLTDKKIIARRAAMELRKGTVVNLGIGAPEFISNVAAEEGITNHFTLTVEAGITGGAP
                     QGGLSFGTSLNPDCIMCQPSMFDYYQGGGLDQTYLGFAEGDGAGNVNVSKFGPKLPGC
                     GGFIDISQNSKQVFFCGTFTASGLKTHVEDGKLVIDQEGRIDKFLKEVEQITFSAKTA
                     LENGIPVVYFTERAVFRLTAQGLELIEIAPGIDLEKDICAHMAVRPAVSPDLRLMDAR
                     IFRDEPMGLK"
BASE COUNT         2640 a         4117 c         4130 g         2475 t
ORIGIN      
        1 tcgcccgcgc gctggcgctg gaccccaaga tcatcctgct ggacgaaccc gccgccggta
       61 tgaaccccaa cgagaccaac agcctgcggg agttcatcct gcagctcaac gccgacgggt
      121 acaccatcgt cgtcatcgaa cacgatatga aattcgtcat gaactcctgc aatcgcatcc
      181 tggtgctcaa cttcgggcag aagatctgcg agggcacccc agaggaggtg cgcggcaacc
      241 gggaggtcaa cgaggcgtat ttcggcaagg gcaagatgct gaaaggtggt ggacacagtg
      301 cttgaagtca gggacctgag cgtgagctac ggctatatca cggcgctggc gggggccagc
      361 tttgaggtga gggaggggga gatcgtcacc ctgatcggct ccaacggggc gggcaagacc
      421 acttcgctga tggcgatttc caacctggtg gacaaagagg agggcagcgt gacttttaag
      481 gggcaggata tcagccgcat gccgcccgcc aagatcgtca ccatgggcat gagccacgtg
      541 ccggaggggc gcaagatatt tccctcgctg acggtgtacg agaacctgat cgccggcacg
      601 ctgggccgcc cgggcatgac cagggcccag gtgcagcggc agctggatac cgtctacagc
      661 ctttttccgc gcctgaagga ccgggcgggg caggggggcg gcaccctgtc gggcggcgaa
      721 cagcagatgc tggcgattgc ccgcggcctg atgatggacc cggatatttt gatgctggat
      781 gagccgtccc tggggctggc gcccatcctg gtggaggaaa tctttgaatt gttggaagag
      841 atccaccgca ccggcaagac catcctgctg atcgagcaga atgcggccat ggccctctcc
      901 atcgcggaca ggggctatgt gctggaaaac ggccgcgtgg cgctcagcgg caccggggag
      961 gagctgctgc acaacgatga ggtgatgcgc gtgtatctgg ggggctgagg cccggcccgc
     1021 gtcgccgcgc gcccgctgcg gagggcaaaa aaagaggcgg ccaaaggccg cctcccccgc
     1081 gccgctttca cagcgccagg gatatggatt ccagatcctt gcgcagggcc gccgccgcgc
     1141 cctcacggtc gctttgctcc atgcagttca caatggccca atgccctttt gaatccagca
     1201 gatcattctg ctccaccgtg tacaggcagt acatggagta ggccatggtc tcggccttga
     1261 ggcagcggtc cagcagcgaa gcgccgaact gattgttgct catgctgtac agcatcttgt
     1321 gaaaagcgat gttgttctcc cagtggttgc gcacggtgag ggtgccgttc tccagcttgc
     1381 ggtcggtctc gaggatatgg gctttgatct gggcgatctg ctcgggggaa atgcgcccca
     1441 ccatcatctc caggttgtcc acctcgatca gcgcccgcag ccggatgatc tcctgcaact
     1501 gtttgttgga gatctgcacg atctcgtagc cgtagcgggg gatgctgcgc agcacgccgt
     1561 cgctgcacag ctcgatcagc gcgtcgcgca ccggggattt gctgacctga tatttttcaa
     1621 ccagcgtctt ttcgttgaac acgtggtcgg cgcggtattc cccgttcacg atcccctccc
     1681 agatcgcgtt ataaatctgt tgtttcagtg tggcagccat tcgaattcct ccgtccccaa
     1741 attaacatta tcattaataa tatcaatgat cgttatttcg aattatagca tcgtttcatc
     1801 tggctgtcaa attttctcag agagcgggaa agaggcgcac tgcggtgaag atgcaaaaga
     1861 aagtagggaa gaaagtgaac aaaagagaac gagtgctggc cgcctaccgc ggtcaaaagc
     1921 ccgaccgggt accctgtgcc ttctggtacc attttcccgc gggatatgag tatggggaaa
     1981 aggcggtgca gatccatctg gattttttcg agagcagcgg gacggacctg tgcaaggtga
     2041 tgaatgagaa ctcctgcccc gacgaccccg ccatccgcac ggccgccgac tggtccggct
     2101 tgaagccgtt cacgatggag gagccgtttg tgcgccgcca gctggaactg gtgcggcgcg
     2161 tgtcacagcg cgtggacggg caggctgtgg tgctggcgac cgtacatggc ctggtggcgt
     2221 cggcctatca catcctgggc gggcctgacc tttatgacgc ggacggcacc gtgctggggc
     2281 gtcacctgcg cgagaacccc gaggggatgc ggcacgcgtt ccggctcatc accgactatc
     2341 tcaaggcgct ctgccgcggc tgtctggagg cgggggcgga cggcatctat ttcgcctcgc
     2401 tgggcgggga gcgccgcatg ttcaccgacg aggagttcgc cgcgttcatc gccccctatg
     2461 agatcgaagt gctggagtcc atccgcgacg tgccctgctt caatgtgctg cacatgtgca
     2521 agtcggacct caatctggag agatatctgg gatatccggc acaggtgctc aactggggcg
     2581 tgtatgagga aaacatctcc ctgcaggagg gccgtgccct ctttggacaa aacaaggtgt
     2641 atctcggcgg gctggacgac cgcgcgggtg tgatcgtgga gggcagcttg gaggagatcg
     2701 gcgccgaggt gcggcggctg ataggggagt ttggttggca caattttatt ttgggcgcgg
     2761 actgcacgct gcccaccggc ctgcccgcag agcggatgca ggcggcggca cgcgcggcgg
     2821 ccctttgaaa agaaaggcgg aaaatgcgat gggatctctg atgaactgga attttggata
     2881 ttatgtgccc acagaggtgg tgttcggctg tggccgggtg gcggaggtgg gcgagctggc
     2941 aaggcgcttt ggcacccgcg cgctgctggt gacagtgccg gagtttgaag cggtgcacgg
     3001 cctgtatgaa aaagtgaaaa ccagcctgcg ggatgcgggg gtggaggtct ttcacttcga
     3061 cggcgtggtg ccgaacccca ccaccgatgt ggtgagcgcg ggggcccgca cagcgaaaca
     3121 ggagaaggtg caggtggtca tcggcctcgg cggcggttcc tcgatggaca ctgcaaaagc
     3181 gatcgccgtg gaggcaagcc atgaggggac ggcgtgggat tacctctatt acaagacccc
     3241 gcccacggac aagacactgc ccatcattgc catcagcacc accgcgggca ccggctcgca
     3301 ggccacctcg gtggcggtcg tcaccaacag cgcaaaaaag gataagtcgg ccctcagcaa
     3361 ctgcaacctt ttcccccgca tcgcagttgt ggaccccgag gtcacggcta ccatgcccgc
     3421 atccgtcacc gctcagaccg ggtttgacgc tttttgccac aactttgaat cctacctgtc
     3481 ggtgcgggcc agcgccatgg tggaggcctt ggccctggac gccatccggc gcgtgatcga
     3541 gtacctgccc cgtgcggtcg gggacggcac agatatggag gcgcgcactc agatggcctg
     3601 ggtcgatacc ctgggcggct tcaccaacgc ctgcgcgggc accacgttac cccacgggct
     3661 gggcatgcag atcggcggcc actgcccgca ggtcacccac ggccaggcct tggccgctgt
     3721 ttatcccagc ttcacccgct atacccggcc tgcggcggtg ggcaagttcg cagccgtggc
     3781 gcgggtgttt gacccggcgc tcgcgggtgt ggacgacgag acggcctccc tgcgctgctg
     3841 tgagctgatt gatgaattct tgaaaaaaat ccacctctgg atcggtctca aagggctggg
     3901 cgtctcgctg caggatgtgc gggaaatcgc cgactgcggc caggtgctgg gcgattacaa
     3961 gcgcaatccc cgcgtggcga ccattgagga gatgtatcaa atcctcatgg aaagctatga
     4021 gcgctgagcg gggaggaatg gaacaatgct gaaagggatt ccctcgattc tgccgccgga
     4081 gctgctcaag atcctgatgg aaatggggca tggggacgag ctggtcatcg gggacggaaa
     4141 ttttcccgcg gcggcgctgg gccagcggct ggtgcgctgt gacggccatg gagtgccgga
     4201 ggtgttggac gcggtgctga ccctgatgcc gctggatttt gtggtggagg cgccggtgac
     4261 cctgatgcac agcaccatgg ggacgccgga tcccccggtc tgggaggagt accggcgcat
     4321 cctgtccctg cgcgagccgg agcaccaagg gatcgccacg gtggagcgct atgaattcta
     4381 tgagagggcg aagcgcgcct acgccgtcgt cgcgacgggc gagaccgcca tgagggggaa
     4441 tatcctcttg aaaaaagggg tctgctgaac cggttggcga gaaacgggag catcaagaca
     4501 atttgtgatt gcagagcaat cacaaacagc cgtgagttgt ttgtcagggc tcggtccctg
     4561 ccggggccac ggacacagca ataaaaaagc caaagacggg ccggtgaacc tgaactgcac
     4621 cccataagtt agacagtatg gtatactgga actaacttat ggggtgattt taatgccaaa
     4681 aagaagagcg aatgcgagct acacagggga gttcaaacaa aaagtaatag aggatatgcg
     4741 caacaatggc ctgagtcaaa acgagacagc ggccaaatat caggtgccac gctcacgggt
     4801 gcagtcgtgg gaacgtatct atctggaaga agggcccgag ggcctgtata tggagcggcg
     4861 tgggcgtggc ggcaagggga agccgaccaa tctggacaag caagtcgaag aggatctgat
     4921 tgcagagaat cagcggctga gggcggaggt agcgtaccta aaaaacttgc aagccttggt
     4981 tttggaagac gagcgacacc agcgcaaaaa atgcaggtga ttcagaaact gaggcaaagt
     5041 tttccgttgg agctgctcct tgatatcgct caactgcccc gcgccaccta ttactaccac
     5101 gtcaagcggc aggcggcgcc ggacaagtac ctggatgctc aggcggccat tacgggcatc
     5161 taccatgaga ataaaggccg ctacggctac cggcgcatca ccgcagacct gcgcaaccga
     5221 ggctttggcc tcaaccacaa gactgtccag cggctgatga agaagctggg cctcgtctgc
     5281 cgggtgcgga tgaagaagta ccattcttac aagggtgaag tgggcaaaat tgtgcctaat
     5341 ctgctggaac ggaactttga ggcgaagaag ccaaatcaga aatgggtgac ggatgtaaca
     5401 gagttccacc tatttgggca gaagctgtac ctatctccga ttttggattt gtgcagcaga
     5461 gacattgtca gttatgccct ttccgatagg ccggggcttt ccatggtaat aaggatgctg
     5521 gatagtgcct ttgcacaaat ctctgacagc acaaacctga tcctccactc cgaccagggc
     5581 tggcagtacc agcacaagca ctaccagcgg atgttgaaga agaaaggtat ccgacagagc
     5641 atgagccgaa aagggaactg tctggacaat gccgtgattg agaacttctt tggcctgctc
     5701 aaaagcgaac tgctctattt gcagaaattc gagtctatgc aacagttcaa ggccgaactt
     5761 attgagtact tagactacta caacaacagc cgtgccaagg caaaactaaa gggcttaccg
     5821 cctgcagttc acagacagca agcccttttg gctgcttgat catatctttg tctaactttt
     5881 tgggtgcagt tcaattgcgg ctgccctttt tacggcggtc cactaaaatt tttcatacag
     5941 gccgctgaac gccacattgg ggttcagctc cttgccctcg gtcaccgtga cgccgtcgcg
     6001 gcccagcgac agggtcaggt tgccctgcgc ctcgcacagc gcgccgccgt ccttatagac
     6061 tgtggcgtcc ccctccaccg tcaggccctt ggcgtccagt acaaagtgga tgaggcccgc
     6121 ctccccgccc tcggtgacgg tgagcaggat ctgccccgcg ctgcccggct gcttggcata
     6181 atcgcccacc caggccgaga catcctgcgc cacctcggag gaggacggcg aagccgaaga
     6241 ggaggaagcg tccttttgcg gggtcgcggt gggcgcggcg gtggctttcg gcgtggcggt
     6301 gggctgcgcc gcagaggagg aggacggccg ggaggaagac gcgccgccgg aggcggaaga
     6361 accgtccccc ccgcaggcgg tgaacagcag acaggccgtg acggccagaa cgatcaactt
     6421 tttcatagag agcacctcgc tttttgcatt ttaaaagcaa acggggcggc ggggccccgc
     6481 cgcgggcctg ccccccttat atcatagaac gaaccggcgc gcgatttcac tactttttgc
     6541 ccgaaccaaa aatttcttcc agcacctggg ccaccccatc ctcccagacc gggggcgcca
     6601 gataggccgt gagctccttg gcctcggggc aggcgttgcc catggcatag ccgcgccccg
     6661 ccgcggccag catctccaga tcgttggggc tgtcgccgaa cgcgaccacg tctttccagt
     6721 cgatgccgtg ccgctgcagc acctggctga tgccggcggc cttggtggtg gccacggggt
     6781 agatgtcgaa gtggtcctgc cactgccgcg cgccctgcag gcggggctgc ccgcgcagat
     6841 agcggtaaac ctcgccctca ttgccgatca gaaaggcgct gaaaggcgct tcgcgcagat
     6901 ggcggtcccg gcgggcggtc tcgtcggtga tgccgccgtc catgccggtg ctctccagat
     6961 acatgcggcg catccgctca aaatcgcagt aagcgccgta cccgctgggg aaagaaaagc
     7021 tcagcgcgcc gccgatacgg cggcagaacg cggccagctc ctccaccagc tccggcgcgt
     7081 agggggtctc gcagaccacg tcgcccccgc ggtccagcac gatgccgccg ttgccgcaga
     7141 cacagtaatc cgccgtctcc cgcagcgccg cgggcaccag ctgccggatg cgccccgatg
     7201 cgaccagcac cagcgccccc tgccgccggg cgcgctccag gcaggccagc aggcgggctg
     7261 tgggctcttt ccgctcccgg tccaccaggg tgccgtccac atcgcaaatc acagcgcgcg
     7321 gcaaagtgtg gttgtactgc aaacgctcac tcctctcccg acgggcctac acgcccgtct
     7381 tcgcctcttt tttctctttc tcttttttgt gcgctttttt cttctttttg cccgccggca
     7441 ccgggatcac cggcagctcg ggcgcgtcca ccggggtctc gtccgcaaag cccttgaagc
     7501 cgcggcccag cacctcgttg acctcggcca tcaccacaaa ggccttttca tccagcccct
     7561 gcaccacctc tttgagatgg taggtctcgc gggggcgcac caccgtcatg aggatgttgc
     7621 gctcatagcc ctgatacata cccacgcccc gctgcagcgt caccccgcgg tgaatctctt
     7681 tttggatggc gtccgagagg gccaccgggt ccttttcgct gatgatatag cacatgttgg
     7741 ccttgtcgaa gccgttgagg atgcggtcgt tcaccatgcc gcagacatag atggtgatga
     7801 tggcgtaaag gcccgcctcg atgtcgcgga acatcagcat cgcgaacagc accacgctgc
     7861 cgtccacgat caaaaacatc ttgcccaccg acatggagcg aaacttgatg agcagcagcc
     7921 gcgccaccag gtcggtgccg ccgttggagg cccccgactt gaggaacagc accgccccca
     7981 ccccgtagat caggccgccg aacaccgccg ccaccagctt gttgtgggtg acggtgggca
     8041 aaaaggagat ggcgtcgagg atgagggcat acaccgtgga cccgcacagc gtgagcaggg
     8101 tgtagcgcgg ccccttgatg aaaaaggcca gcagaatgaa cgggatgttc agcaaaaaga
     8161 caaaggtgcc gatggacacc ggcaaaaagg aggacagcac gatggcgatg ccggaaaagc
     8221 ctcccgccgc gatgttgttg cccgccagga acagctggat ggcaaacgcg taacagccgt
     8281 tcgccaacag aaaatagatg atctttcgca cctcgttcgc aaaacggaat ttttgctttg
     8341 ccacggccgc ggcctccttt tcgggcgccc tcaaaacgcg gcgctccgcg ccggcccagc
     8401 gcggggccgg cttttacagc actagcatag cacgccgggc ggtacggtgc aacaactccc
     8461 cgccctgcgg gcgcggaaat agtcgtttcg ccaaaagacg cccgccctct gtggggaatt
     8521 gcccgacact tttacccgcc ctgtcggccc gtcgcagaca gcaaaaggcc catgaaaggg
     8581 aatccccctc cataggccgg tatctcaaac gcggtcaagg gccttgccgc cgcagggccg
     8641 cggtctcttt tcaagcggcg cgccgcccgc cgcgcggccg ggcgcatccc ttttcggctc
     8701 cgtgccggcg gggcggcagc gccttttcaa gcgctctgca aagctgctca ctcgatctcg
     8761 atggcgcggg ccgcgggcgc gggcgcggcc tcctttttgg gcagcgtcag ctccagcacg
     8821 ccgtttttat aggcggcttt gatctcctcc tgacggatgg tggagacatc gaagctgcgg
     8881 ctgtacgagc catagtggcg ctcgcggcag acatagctgc cgtctttctc ttttttctcg
     8941 ctctcctcgc tgtgcacggc cgagatggtc agcacgtcgc cgtccagatc cagcttgata
     9001 tcctctttgt tgaagccggg cagctcggcg ctgagcaggt agctgtcgcc cttgtcctcg
     9061 atatccgtcc tgcagctcag ggccggcata gacggcatag acggcatcga cttttcgccg
     9121 aagaaatatt tgtccagatc gctgaacaga tccatcatcc cgcgctcgcg gcgctcataa
     9181 ggcataagac caaacataaa gcattcccct ttcgcttgca tcgttgtttt ttgttgtaaa
     9241 cctgaaaccc cggcggatca actccgcccg ctctcgcggc acatgcccga tcacctgttt
     9301 ccctccgcgc tgttcaaagc gccgtcgttt tttgtttacg gctatagtat atcgccggca
     9361 tgttaaaaaa caacggatga tttttgaaca aactgtaaaa agttagcact cttatattta
     9421 gagtgctaac tttttataaa acatcattat tcggcttttt tcaggcgccg caggatgtaa
     9481 aacccatttt ttcagcggga gtgccaaccc atttttaccg ccctaaaatt tcgccgatgg
     9541 cgccgtggaa caggtggtcc gcccgccggt cggccggggt gggggtcttg ttgatgacca
     9601 ccagcttgtg gccccggtaa tagtcgatca ggcccgccgc cgggtagacg gcaagcgagg
     9661 tgccgccgat gatcagcagg tcgcaggccg cgatggcccg caccgcgccc tccagcaccg
     9721 cgccgtccag gccctcctca tacagcacca cgtccggctt gatgacgccg ccgcagctgc
     9781 agcgggggat gccgcggctc tctttcaccg cctccacgcc gtaagccctg ccgcagcgca
     9841 cgcagtggtt gcgcagcaca ctgccgtgca gctcaaaaac gtgtttactg cccgcgcgct
     9901 ggtgcaggcc gtcgatgttc tgggtcacca cggcgcggca tttgccccgc gcctccagct
     9961 ctgccagcat ccggtgcgcc gcgttgggct gggcatccag atagagcatc ttgtcgcggt
    10021 aaaaatcaaa gaactcttcg gtatggcgct caaaaaaggt gtggctcagg atcgtctcgg
    10081 gcgggtaggc gtacttttgg tggtaaagcc cgtccacact gcgaaaatcc gggataccgc
    10141 tctcggtgga gaccccggcg cccccgaaaa agacgatatt gccgctgccc tcgatgagct
    10201 ctctcagttt tgcgtccatc agcttcccct cctcgtgccg ccccggcgca ccctgcgtcg
    10261 gagcgcggcc gccgccagcg ccagatagcc cagcgccagg tacagcagcg gataaaacgg
    10321 ctcccacatc ctcagcacca gcccgtagcc gaagatgaaa agggccgcca gcaacgcggg
    10381 cagtggccag cggctgcgcg gccgccgtcc gcaaaaagcc ccggcggcca gaaaaaagac
    10441 cgggtgcacc gccagcagaa aaagcatgga aaaggccatg cccgcctcgg gcggcagcag
    10501 ataccgcgcc agcgccggca ccccggcaaa gagcgccagc cccaggccca gcgcccagag
    10561 cgtctctttt ttgtccatcc ctgattcccc tccctcttcc gcccgcggcg ccagcccccc
    10621 gccccgtgtg gtgtgaattg ggccgtggcc gagggggcgg tctcttcggc ccgcgacggc
    10681 gccgaggccg ccctccgccg cgccgccgcg gcctgtccgc gaaatggata aaacgccgcg
    10741 gcgtttctgt cccgttttta ttatacttct aaaaaagcgt cggcacaatg tcccgcgcat
    10801 tgactttgta aacctacttt gttataattt taacatatga gacgaatctc cgcatcccgg
    10861 ccgcccgcaa caggcggccg gggcgcatgg ccgggtcttc gggcacggcc ccaaaactga
    10921 taaaaaggga tgggatcaca atgtcagtca agatcatgac cgcccgcgag gcgatggacc
    10981 tggtgccgga cggcgccacc gtggccaccg gcggctttat cggcgccagc ttcgccgagg
    11041 agctggcgct ggagttagag aaccgctttc tcgagaccgg gcacccgcgc gacctgtcgc
    11101 tgatgttctg cgccgcccag ggcgaccgca aggacaaggg gctgaaccac ctggctcacg
    11161 agggcatgct gaaaggcgcc atgggcgccc actggggcct ggcccccaag attggccaga
    11221 tggttgccga caacaagatc ctggcctatg tgttcccgca gggggtcatg tgccatatgc
    11281 tgcgcgactc ggccgcccac cggcccggca ccctcaccca tgtgggcctt ggcacctttg
    11341 tggacccgcg tctcggcggc ggcaagatca acgatttgac ccgcgccgcg ccggaccggg
    11401 tgcacctgat gcctctgggc ggcaaggaat atctgttcta tgactaccaa aaggtggatt
    11461 tcgcgctgct ttgcggcacc tacgccgacg agaacggcaa catcagcatg gaacgctgcg
    11521 ccgtcaccgg cgagatgctg gccaccgccc aggcctgcaa gaacagcggc ggcaccgtgg
    11581 tggtacaggt ggagcgggtg gtcaaggccg gctcgctgga ccccaaaaag gtggagatcc
    11641 ccggcattct ggtggacgcg gtggtgccgg tgagcgacat gaagtaccac atgcagacct
    11701 ttgacgtgga gtataacccc gccctctccg gcgagcaccg ggtcagcctg aaagcggccg
    11761 agcccctgcc cctgaccgac aaaaagatta tcgcccgccg cgccgccatg gagctgcgca
    11821 agggcaccgt ggtcaacctg ggcatcggcg ctccggagtt catctccaac gtggccgccg
    11881 aggagggcat caccaaccac ttcacgctga ccgtggaggc aggcatcacc ggcggcgcgc
    11941 cccagggcgg gttgtctttc ggcaccagcc tcaacccgga ctgcatcatg tgccagccca
    12001 gcatgttcga ttactaccag ggcggcggcc tggaccagac ctatctgggc tttgccgagg
    12061 gcgacggcgc cggcaacgtc aacgtctcca agttcggccc caagctgccg ggctgcggcg
    12121 gctttatcga catcagccag aactcgaagc aggtcttttt ctgcggcacc tttaccgcca
    12181 gcggcctcaa gacccacgtg gaggacggca agctggtgat cgaccaggag ggccgcatcg
    12241 acaagttcct caaagaggtg gagcagatca cgttcagcgc caagaccgcg ctggagaacg
    12301 gcatcccggt ggtctacttc accgagcggg cggtcttccg gctcaccgcc caggggctgg
    12361 agctgatcga gatcgccccg ggcatcgacc tggaaaagga catctgcgcc cacatggcgg
    12421 tgcgcccggc cgtctccccg gacctgcggc tgatggacgc gcgcatcttc cgggacgagc
    12481 cgatgggctt gaaataagcg gaggatgagc agggccggtc agggcgtttt gtcatgaccc
    12541 tgcaaaaaaa gggaacggcc ccggcgcgtt gagataaaaa atagccgcag gctatttttt
    12601 cggccatccg gccagtcctc tggccggggc catcaagata agttgttttt tattgcgaag
    12661 caataaaaaa atagccgcag gctatttttc ggtcctccgg cccgtcccgg gccggagcca
    12721 ctgagataag tcgttttttt attgcggagc aataaaaaaa cattggtttt gggccgacgg
    12781 tcttgacgcc cccgcccatt caaaacagac tcaaaagggt tgacgatccg caaagatcgc
    12841 caaccttttt tgcactgtgc ttcctccaaa accgttggta caaaactgcc gcgtccccct
    12901 tgagagggaa agctgtgatg cagagccggg aaaaatccgc gtcgattgcc cgcggtgacg
    12961 ggtgcttgcc tgcccgcgcg ggcatttcac cgggcgaagc cgcaaacccc tccgtcgccg
    13021 cggggtgaat ttgacggtgc tgcgccccct cggggacaag gtttttctgc gaaaacgcgc
    13081 ctttccggct gtctcccctt tttggcgcac cgggccttgc gccgtccttg ggcggcacgg
    13141 ccccggcgca aattctttca gcccgtgcag gtctctcgcc ccgcgcagca gccaaagagc
    13201 gcgccgccgg caccctcgcg ggcggcgtgc cctgcgcccc gtcccgcgca aattcccggc
    13261 cctgtgcacc gccctggcgc gcgtctcccg cctcgtgcag gtccccgcct ctaaaagcac
    13321 gctgccgggg gggggcgcgc gggcgcgggc ggttccatcc cc
//
LOCUS       DBMDYA010000005        12595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1443_length_12595_cov_1.491436, whole genome shotgun sequence.
ACCESSION   DBMDYA010000005 DBMDYA010000000
VERSION     DBMDYA010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_12595_cov_1.491436"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="ACZPFK_00310"
     CDS             <1..1209
                     /locus_tag="ACZPFK_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014805.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1404781.1"
                     /translation="ENAENRDCFEKIMERLQIPQPKGEAVTNIEDGCRVAARIGYPVL
                     VRPSYVLGGRAMQIVADEEALRRYLQTAVELDADQPVLVDKYILGKELEVDAICDGKD
                     VFVPGIMEHVERTGVHSGDSISVYPTFSVSQKVKDTILDYTRRLGLGIGIVGLFNIQF
                     IAVGDAVYVIEVNPRSSRTVPFLSKATGIPIAKIATKVILGQGLAGQGYTELCAPERK
                     KWYVKAPAFSFSKIRGLDAYLSPEMKSTGEAIGYDRSLTRALYKALQASGMNVANYGT
                     IFVTIADKDKEEALPLIRRFYDLGFNIEATEGTAAFLKEHGIRTRSKKKISEGSEEIL
                     ESLRRGHVNYVINTSVIGGAGAETDGYEIRRCAVENGVTMFTALETVKVLLDVLEEIT
                     LGVSTIDSDE"
     gene            1353..2096
                     /locus_tag="ACZPFK_00315"
     CDS             1353..2096
                     /locus_tag="ACZPFK_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1404782.1"
                     /translation="MQKQGIYTIQENAPLTRDVYRMVLAGDTSAVTAPGQFVNILIDG
                     CYLRRPISIADWDESTLTLIYKVVGRGTRRMADMGGPGAALDLLVGLGNGYDLAACGA
                     APVLLGGGVGVPPLYGLARRLWQQGCTPTVLLGFNTAADVFYEREFQALGCPTLVATA
                     DGSYGQKGFVTDLLRTAGLRCDYFYTCGPEPMLRAVYDACACPGQLSFEERMGCGFGA
                     CMGCSCKTKYGAKRICKDGPVLRREEVIW"
     gene            2090..3001
                     /locus_tag="ACZPFK_00320"
     CDS             2090..3001
                     /locus_tag="ACZPFK_00320"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1404783.1"
                     /translation="MVDLRVELSGLALDNPVIPASGTFGYGNEFKDFYDLDILGSFSF
                     KGTTKEPRFGNLTPRIAECNAGMLNAVGLQNPGIDAVIREELPRMKGYFHKPVIANIG
                     GFSIDEFRYCCERVDKEPQVGLIEVNVSCPNVHEGGVNFGSTPEGAAAVTRAVKQVAS
                     KPVYLKLSPNVTDIVAVAKACEEAGADGLSLINTLLGMRIDLARRRPVLANTMGGLSG
                     PAIFPVALRMVWQVAHAVEIPVIGMGGVDSAEKVAEMMMAGATAVQVGAANLVDPFAC
                     KKIIEALPGVLAKLGVERAADLTGMAG"
     gene            3432..4130
                     /gene="pyrF"
                     /locus_tag="ACZPFK_00325"
     CDS             3432..4130
                     /gene="pyrF"
                     /locus_tag="ACZPFK_00325"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1404784.1"
                     /translation="MDRDVIIACDFAGAAETYAFLDRFGAERPFVKIGMELFYSEGPE
                     IVRELKRRGHRIFLDLKLHDIPNTVKKAMAALSRLDVDICNLHAAGTIEMMKYALEGL
                     TRPDGSRPLLIAVTQLTSTSEERMRRELLIDRPINDVIVKYAENAKKAGLDGVVCSPL
                     EAGMVKEACGSGFLTVTPGIRFADGEAGDQVRITTPARARQIGADYIVVGRPITAAAD
                     PLAAYRRCVAEFMG"
     gene            4166..4678
                     /locus_tag="ACZPFK_00330"
     CDS             4166..4678
                     /locus_tag="ACZPFK_00330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1404785.1"
                     /translation="MTCKIRKWQLSDARDLASALSNTKIQDNLRDGLPYPYTEKDAVE
                     YITAMLSADENETFAFAITIDDKVIGSIGAFRQGNIHRRTAELGYYLAEDSWGKGIMT
                     QAVKQICNYVFDKSDVIRIYAEPFAYNTASCRVLEKAGFQYEGTLRKNAVKNGRVVDM
                     KMYALLKTES"
     gene            complement(4879..5076)
                     /locus_tag="ACZPFK_00335"
     CDS             complement(4879..5076)
                     /locus_tag="ACZPFK_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404786.1"
                     /translation="MAGVKNFGKPAAALAEPGWLKKTFIRDSASPVICPCDFCKEDKE
                     MRLKENPILVKSSAHKTKAEQ"
     gene            5136..5777
                     /gene="pyrE"
                     /locus_tag="ACZPFK_00340"
     CDS             5136..5777
                     /gene="pyrE"
                     /locus_tag="ACZPFK_00340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1404787.1"
                     /translation="MLDTENRIAKDLLSIGAVFLRPDEPFTWASGIKSPIYCDNRLTL
                     SAPSVREEIENGLAELIEEFYPGAEVLMGTSTAGIAHAAITATILDLPMGYVRGGSKD
                     HGRKNQIEGKLEPGQKVVVVEDLISTGGSVLEVVDVLRAAGAEVLGVVSIFTYGMKKG
                     LERLAADKVENHSLSNLDALVKVAAAEGYIRESDCEKLLAFRDNPSDESWMKR"
     gene            5803..6972
                     /locus_tag="ACZPFK_00345"
     CDS             5803..6972
                     /locus_tag="ACZPFK_00345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1404788.1"
                     /translation="MDKNEIRALAEGQREYMVGLRREFHRHPEVAGDEVWTSARIAQE
                     LTALGIPFVVDERRNVIGRIEGARPGKRLALRADFDALPMTEATGLPFASENPGVMHA
                     CGHDGHTAGLLGAAKLLWGMRGQLAGTVYLCFQMGEEQGWGAAEIIRYLKAQGGVDLA
                     AGIHLMPEWQPGTFVCMPGPFMAGTAGWDITVTGRGGHGSAPWNTVDPIRPAAEILLR
                     LGAVQGTRRSAFDHFVISPCMFHAGTGKNIVPDTAVLSGTNRFFSVQQLDELFALMEG
                     MTRDVAASYGATAELVRGPATLPVVNDAGAVALAAEAVGRIGGRVEQMVPSLATDDMG
                     DYLQEFPGVYVFGGSAPLQGERLPIHNTAYTINEDALCWSAAFFTALACGFLSEK"
     gene            7289..8443
                     /locus_tag="ACZPFK_00350"
     CDS             7289..8443
                     /locus_tag="ACZPFK_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HIR1404789.1"
                     /translation="MKIAWFCIPAHGHTNPTLALVKEMTDAGHEVYYFSFALFREKIE
                     RAGAHFISCDGCDFEMEDQENADRIGKDMAFATELLVSAALALDSMVSAQIALIKPDV
                     IVADSIAYWGKLTAMKYKIPYISSTTTFAFNRYSSRYMRRKVWETIKMLLMMPKINRQ
                     IRRLQENGYPVKGILDIVQNDNETDTIVYTSSFFQPCADTFSDRYHFIGPSIRPVESA
                     VKKLAEKTIYISMGTVNKNKTFYFNCIEALKTTGYQIIISMGTNRDCLGDLPDQIQVY
                     DFVDQMAVLSISDVFITHCGMNSASEGLYFEVPLILFPQTPEQGAVAARVQELGAGVR
                     LGSIDKEEIARAVDRVLKEPVYRENAVRISKSFKACGGVREAKEFIESKG"
     gene            8613..9323
                     /locus_tag="ACZPFK_00355"
     CDS             8613..9323
                     /locus_tag="ACZPFK_00355"
                     /inference="COORDINATES: protein motif:HMM:NF020922.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR1404790.1"
                     /translation="MPFSQKSLDFLFENRLHDSKEWFLEHKNEYETLVKAPLVELSAA
                     LGPVMLELDPQLTTDPAVGKTISRIRRDTRFTHDKSLYRDNMWIVYKRGKMYGREVPG
                     LYFEVFSDGHFGYGCGFYDASTAYMETLRAQVRGQTPAWKKADKALRAQQVFALEGER
                     YKRPRFPDAPAAQRDWLELRGISFNAQRHDPGLLFSDRLAAELAEDFRLLAPLYGFLL
                     EVAGQQRAEALQRPWELA"
     gene            complement(9379..9651)
                     /locus_tag="ACZPFK_00360"
     CDS             complement(9379..9651)
                     /locus_tag="ACZPFK_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1404791.1"
                     /translation="MKADKTQITRLLKTARGQIDGVLRMVDDDKYCIDIANQIMAAEA
                     ILRKANKEIIRAHLEGCVKEAFSSGSEERMEQQIDEILNVMDSLGK"
     gene            complement(9737..12325)
                     /locus_tag="ACZPFK_00365"
     CDS             complement(9737..12325)
                     /locus_tag="ACZPFK_00365"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1404792.1"
                     /translation="MKQKFDVTGMTCSACSAHVEKSVRKLPGVSSVAVNLLQNTMMVE
                     YDGDALSDAQICDAVKAGGYGASPQQPHGAGAKCAAAPKEAPGGAMAAELAGMKRRLI
                     VSFCFLVPLMYVSMGHMMGLPLPAFLLGHENAVSFAMTQLLLTLPIMYVNRKYYEVGF
                     KTLFRGAPNMDSLIAIGSAAAVVYGLVAIYGIGYGLGHGDMELVGHYHMDLYFESAGT
                     ILTLITVGKYLETRSKGRTSDAITKLLDLAPKTAVVERDGEEIEVPVEEVVVGDTVVV
                     RPGGRIPVDGVILEGNAALDESALTGESLPVEKGPGDKVISASINQSGYLKCKALKVG
                     DDTTLAQIVQLVEDANASKAPIAKLADKVSGVFVPVVITIAVVAAAVWLLLGYPVDFA
                     LSIGIAVLVISCPCALGLATPTAIMVGTGKGAENGILFKSAESLEVAHTVNTVVLDKT
                     GTVTEGKPEVTDLLPAPGADPRELLRVAAAAERPSEHPLAAAILRRAGAQGLEPDAAS
                     DFATVPGQGVTGKVDGKPVLAGNLRMMEANGLSADRFGVDADALANQGKTPLFFARDG
                     EPLGVIAVADVVKPTSRAAIEEFKALGVDVVMLTGDNKRTAEAIRGQLGIDRVVAEVL
                     PQDKEREVAALQQAGKKVAMIGDGINDAPALARADVGVAIGAGTDIAIESADVVLMKS
                     DLLDAVTAFQLSRSVIRNIKQNLFWAFFYNSIGIPLAAGVFYTLLGWKLNPMFGAAAM
                     SLSSFCVVSNALRLKLFKPRFRHPLPSSGSGCPIAAAAPSEIENDKKEKEVSVMNKTL
                     KIKGMMCNNCKAHVEKALAAVEGVSAVTVDLEAAQAVVTLAADVADEKLVTAVTEAGY
                     EVVSVQ"
BASE COUNT         2398 a         3773 c         4006 g         2418 t
ORIGIN      
        1 gagaacgccg agaaccgcga ctgctttgaa aagatcatgg agcggctgca gatcccccag
       61 ccgaaaggcg aggcggtgac caacatcgag gacggctgcc gcgtggccgc ccgcatcggc
      121 tacccggtgc tggtgcggcc cagctatgtg ctgggcggcc gggccatgca gatcgtggcc
      181 gacgaggagg cgctgcgccg ctatctgcag accgccgtgg agctggacgc ggaccagccg
      241 gtcctggtgg acaagtacat cctcggcaag gagctggagg tggacgccat ctgcgacggc
      301 aaggacgtct ttgtgcccgg catcatggag catgtggagc gcaccggcgt gcactcgggc
      361 gactcgatca gcgtctatcc cacgttcagc gtcagccaaa aggtgaaaga caccattttg
      421 gactacaccc gccgcctggg cctcggcatc ggcatcgtgg gcctgttcaa catccagttc
      481 atcgcggtgg gggacgccgt ctatgtcatc gaggtcaacc cccgctcctc ccgcaccgtg
      541 ccgtttttgt ccaaggccac cggcatcccc atcgccaaga tcgccaccaa ggtgattttg
      601 ggccaggggc tggcggggca gggctatacc gagctctgcg cgcccgagcg caaaaaatgg
      661 tatgtcaagg ccccggcgtt ctcgttctcc aagatccgcg gcctggacgc ctatctctcg
      721 ccggagatga agtccaccgg cgaggccatc ggctatgacc gcagcctgac ccgcgccctc
      781 tacaaggcgc tgcaggccag cggcatgaat gtggcgaact acggcaccat cttcgtcacc
      841 atcgccgaca aggacaagga ggaggcgctg cccctcatcc gccgctttta cgacctgggc
      901 ttcaacatcg aggccaccga gggcacggcg gcctttctga aagagcacgg catccgcacc
      961 cgctcgaaaa agaagatcag cgagggcagc gaggagattt tggagtcgct gcggcgcggc
     1021 catgtcaact acgtcatcaa caccagcgtc atcggcggcg ccggggccga gaccgacggc
     1081 tacgagatcc gccgctgcgc ggtggaaaac ggcgtcacca tgttcacggc gctggagacg
     1141 gtaaaggtgc tgctggacgt gctggaggag atcacgttgg gcgtgtccac gatcgacagc
     1201 gacgagtgag tggggcagct gtgggctgcg ctctccgggg ccaacgcgct tttccgccga
     1261 agtgatattt tttcaggcgt tcctgcggcg cgccgcgccg tttcgccgcg acgacctctc
     1321 gtaagacgac ctctcgtaaa aggaagatca gcatgcaaaa acagggaatt tacaccattc
     1381 aagaaaacgc ccccctcacc cgcgatgtct accgcatggt gctggcgggg gacacctccg
     1441 ccgtcaccgc gcccgggcag ttcgtcaata tcctcatcga cggctgctat ctgcgccgcc
     1501 ccatctcgat tgccgactgg gacgagtcca ccctcaccct catctacaag gtggtggggc
     1561 ggggcacccg ccgcatggcg gacatgggcg gcccgggggc cgcgctggat ctgctggtgg
     1621 gcctgggcaa cggctatgat ctggccgcct gcggcgccgc gccggtgctg ctgggcggcg
     1681 gcgtgggcgt gccgccgctc tacgggctgg caaggcggct ctggcagcag ggatgcaccc
     1741 ccaccgtgct gctcggcttc aacaccgccg cggacgtctt ttacgagcgg gagtttcagg
     1801 ctctcggctg ccccaccctg gtggccaccg cggacggcag ctatgggcaa aagggctttg
     1861 tcaccgatct gctgcgcacg gcgggcctgc ggtgcgacta tttttatacc tgcggtcccg
     1921 agccgatgct gcgggcggtg tacgacgcct gcgcctgtcc cggccagctg agctttgagg
     1981 agcgcatggg ctgcggcttc ggcgcctgca tgggctgcag ctgcaagacc aaatacggcg
     2041 ccaagcgcat ctgcaaggac ggcccggtgc tgaggaggga ggaagtgata tggtagacct
     2101 gcgcgtggag ctctccggcc tggcgctgga caacccggtg atcccggcca gcggtacctt
     2161 tggctacggc aatgagttca aggattttta cgacctggac attttgggct cgttttcatt
     2221 caagggcacc acaaaggagc cgcgcttcgg caacctcacc ccccgcatcg ccgagtgcaa
     2281 cgcgggcatg ctgaacgcgg tggggctgca aaaccccggc atcgacgccg tcatccgcga
     2341 ggagctgccc cgcatgaaag gctattttca caagccggtc atcgccaaca tcggcggctt
     2401 ttccatcgac gagttccggt actgctgtga gcgcgtcgac aaagagccgc aggtgggcct
     2461 gatcgaggtc aacgtcagct gccccaacgt ccacgagggc ggggtcaact tcggcagtac
     2521 gcccgagggc gcggcagccg tgacccgggc cgtcaagcag gtcgcctcca agccggtgta
     2581 tctgaagctc tcccccaacg tcaccgacat cgtggcggtg gcgaaagcct gcgaagaggc
     2641 gggggcggat ggcctctccc tcatcaacac gctgctgggc atgcgcatcg acctggcccg
     2701 ccgccggccg gtgctcgcca acaccatggg cggcctttcc gggccggcca tctttccggt
     2761 ggcgctgcgc atggtctggc aggtggccca cgcggtggag atcccggtga tcggcatggg
     2821 cggcgtggac agcgccgaaa aggtggccga gatgatgatg gccggcgcca cggcggtgca
     2881 ggtgggcgcg gccaacctgg tggacccctt tgcctgcaaa aagatcatcg aggccctgcc
     2941 cggggtgctg gcgaagctgg gggtcgagcg ggccgccgac ctcaccggca tggccggttg
     3001 aggacaagac cccttgcggg caagaccctt taagggcaag actccacttt ttgaaaaaag
     3061 tggacaaaaa cttttgcctg ctttttcggc catccggccg gtccccccgg ccggggccat
     3121 tgagatagat cgttttttat tgcgcagcaa taaaaaatag ccgcaggcta ttttttcggc
     3181 catccggcca gtcctctggc cggggccatc aagataagtt gttttttatt gcgaagcaat
     3241 aaaaatagcc gcaggctatt ttttcggcca tccggccagt cctctggccg gggccatcaa
     3301 gataaattgt tttttattgc gaagcaataa aaaatagtat tatactggaa taaacctaca
     3361 aagagtattc aaagggcggc cgcgcgccgc gcgagaaatg ggaacaaaac agaaaaaagg
     3421 agaccagaga gatggacaga gacgtcatca ttgcctgcga ttttgccggc gccgccgaga
     3481 cctatgcctt tttggaccgc ttcggggcgg agcgcccctt tgtcaagatc ggcatggagc
     3541 tgttctattc cgaggggccg gagatcgtgc gggagctcaa gcgccgcggt cacagaatct
     3601 ttttggacct gaagctgcac gacatcccca acacggtgaa aaaggccatg gccgccctct
     3661 cccgtctgga tgtggacatc tgcaacctgc acgccgcggg taccattgag atgatgaagt
     3721 acgcgctgga ggggctcacc cgcccggacg gcagccgccc gctgctgatc gccgtgaccc
     3781 agctcacctc caccagcgag gagcggatgc ggcgcgagct gctcattgac cggcccatca
     3841 acgacgtcat cgtgaagtac gcggagaatg caaaaaaggc ggggctggac ggcgtggtct
     3901 gctccccgct ggaggcgggc atggtcaagg aggcctgcgg cagcggtttt ctcaccgtga
     3961 cccccggcat ccgctttgcc gacggcgagg cgggggacca ggtgcgcatc accaccccgg
     4021 cccgggcgcg gcagatcggc gccgactata ttgtggtcgg ccgccccatc accgccgccg
     4081 ccgacccgct ggccgcctat cgccgctgcg tggcggagtt tatgggctga aacctttgcg
     4141 gaagcgatag aaaagggggg atcacatgac ttgtaaaata agaaaatggc agttatcgga
     4201 tgcaagagat ttggcgtcgg ctctttccaa tacaaaaata caggataatc ttcgggatgg
     4261 attaccttac ccctatacgg agaaagacgc agtggagtat attactgcca tgctgtccgc
     4321 agatgaaaac gaaacgtttg cgtttgccat tacgatagac gataaggtaa ttggcagtat
     4381 tggcgctttt cgtcagggaa acattcacag gcggactgct gagctggggt attacctcgc
     4441 agaagatagt tggggcaaag gcatcatgac gcaggccgtg aaacaaatct gcaactatgt
     4501 ttttgataaa agcgatgtga tccgtatcta cgcagagccg tttgcataca ataccgcgtc
     4561 ttgccgggta cttgaaaaag cgggttttca atatgagggt accttgagaa agaacgccgt
     4621 caagaacggc agagtggtcg acatgaagat gtacgcactg ctgaaaacag aatcataaca
     4681 ttcagcttgc agtttaccct gcctgatcgt aagcgaggct tcttcgggcc gggttatctt
     4741 cttcaacaca cttttttatg ggactgtttt ttaaacaaag aatgtttttg tcagcgctcg
     4801 cgctcatttt ttgacggata gagttgaaat tgtatcgatg cctttgttgc aaaatagaag
     4861 ttgacaaccc cgtttcgttt attgctctgc tttcgtctta tgggcagagg acttgacgag
     4921 gatcggattt tctttgagcc tcatttcttt atcctcttta cagaagtcac aaggacagat
     4981 caccgggctt gcgctgtccc ggataaaagt ttttttgagc cagccgggct cggccaaggc
     5041 ggcggccggt tttccgaaat ttttcactcc cgccaactaa aaaacttttc aaaaagccgc
     5101 tctcaacacc tgcgcacaga aaaggagaaa ttatcatgct ggacactgaa aaccgcattg
     5161 caaaggacct gctctccatc ggggccgtct ttctcaggcc ggacgagccc tttacctggg
     5221 cctccggcat caagagcccg atctattgcg acaaccggct gaccctgtcg gccccgtctg
     5281 tgcgcgagga gatcgaaaac ggcctggctg agctgatcga ggagttttat cccggcgccg
     5341 aggtgctgat gggcacctcc accgcgggca tcgcccacgc cgccatcacc gccaccattc
     5401 tggacctgcc catgggctat gtgcggggcg gcagcaagga ccacggccgc aagaatcaaa
     5461 tcgagggcaa gctggagccg ggccagaaag tggtggtggt ggaggacctg atctccaccg
     5521 gcggcagtgt gctggaggtc gtggacgtgc tgcgcgccgc cggggccgag gtgctggggg
     5581 tggtctcgat cttcacctac ggcatgaaaa agggcctgga gcgcctggcc gcggacaagg
     5641 tggagaacca ttccctctcc aacctggacg cgctggtcaa ggtggcggcg gccgagggat
     5701 atatccggga gagcgattgc gaaaagctgc tggcctttcg cgacaacccg tccgacgaga
     5761 gctggatgaa acggtaagag caaaaaagga gggtgcgctg agatggacaa aaacgagatc
     5821 agggcgctgg ccgagggcca gcgggagtat atggtgggcc tgcggcggga gtttcaccgc
     5881 cacccggagg tggcaggcga cgaggtgtgg acctccgcgc gcatcgcgca ggagctgacc
     5941 gccctgggca ttccgtttgt ggtggatgag cgccgcaacg tcatcggcag gatcgagggt
     6001 gcgcgcccgg gcaagcgcct ggctctgcgg gcggattttg acgccctgcc catgaccgag
     6061 gccacgggcc tgccctttgc ctccgagaac cccggcgtca tgcacgcctg cggccacgac
     6121 ggccacaccg ccgggctgct gggcgcggca aagctgctgt ggggtatgcg cgggcaactg
     6181 gcgggcacgg tttatctctg cttccagatg ggggaggagc agggctgggg cgccgccgag
     6241 atcatccgct acctcaaggc ccagggcggc gtggacctcg ccgcgggcat ccatctgatg
     6301 cccgagtggc agcccggcac ctttgtctgc atgcccggcc cctttatggc cggcacggcg
     6361 ggctgggaca tcacggttac cgggcgcggc ggccatgggt ccgccccctg gaacacggtg
     6421 gaccccatcc gcccggcggc ggaaattctg ctgcggctgg gcgcggtgca gggcacccgg
     6481 cgctcggcgt tcgaccactt cgtcatcagc ccctgcatgt tccacgccgg caccggcaag
     6541 aacatcgtgc ccgacaccgc ggtcctcagc ggcaccaacc gctttttctc ggtgcagcag
     6601 ctggacgagc tgtttgcgct gatggagggg atgacgcggg atgtggccgc gtcctatggg
     6661 gccacggcgg agctggtgcg cggcccggcc accctgccgg tggtgaacga cgccggggcc
     6721 gtggccctgg cggccgaggc ggtggggcgg atcggcggcc gggtggagca gatggtgccc
     6781 tcactggcga cggacgacat gggggattac ctgcaggagt tccccggcgt ctacgtcttc
     6841 ggcggcagcg cgccgctgca gggcgagcgc ctgcccattc acaataccgc ctacaccatc
     6901 aacgaggacg ccctgtgctg gagcgccgcc tttttcaccg ctttggcctg cggctttctc
     6961 agcgaaaaat aggcgcaggc cgcttttgaa ccttttgggc tgtcccggtt tgagcctgta
     7021 gggagcggtt tgtttttttc ggccgtccgg gccgcggaga cgggtgatgg aagctcccgc
     7081 cgtgggttca tgagacctgt ttcacagagt gcgcggagcc atggagatac cgtgtcttta
     7141 tggctccgcg cactttggcg ccgctgtgaa accggcgttt gccgtttacc ggcggccctt
     7201 gcgcgcagga tcaaatgaat tgagaaattg tagggaatag agtataatct ggtcgagtgg
     7261 caaaaagagg cgcttgtgga ggtgcaaagt gaaaattgcc tggttttgta tccccgcgca
     7321 tggacataca aaccccaccc tcgctttggt gaaagaaatg acggacgcgg ggcacgaggt
     7381 atattatttt tcatttgcgc ttttccggga gaagatcgaa agggcgggcg cgcattttat
     7441 cagctgcgac ggctgcgatt ttgaaatgga ggatcaggag aacgcggaca ggatcgggaa
     7501 agatatggcc tttgccaccg agctgctggt gagcgcggcc cttgctttgg acagcatggt
     7561 gtcggcgcag attgctttga taaaaccgga tgtcattgtg gcggattcca tcgcctattg
     7621 gggaaaactg acggcaatga aatataagat cccctatatt tcctccacga ccacctttgc
     7681 ctttaatcgg tattcctcca gatatatgag gcgcaaagtg tgggaaacga taaaaatgct
     7741 cctcatgatg ccaaaaatca acagacagat tcggcggctg caagagaacg ggtaccctgt
     7801 caaagggatt ctggacattg tacaaaatga caacgaaacg gacacgatcg tttacacctc
     7861 cagttttttt cagccctgtg cggacacttt ttcagacaga tatcatttca tcggcccctc
     7921 catccggccc gttgaaagcg cggtaaaaaa actggctgaa aaaacgatct acatttcgat
     7981 gggcacggta aataaaaata agacttttta tttcaactgc atagaggctc tcaaaacaac
     8041 gggatatcaa atcatcatct ccatggggac aaacagagac tgcttgggcg atctgccgga
     8101 tcagattcag gtgtatgact ttgtggatca aatggcggtg ttgtcgattt ctgatgtttt
     8161 tatcacccat tgcggcatga actcggcctc agagggattg tattttgagg tacccttgat
     8221 cctttttccg cagacgccgg aacagggcgc ggtggcggcc agggtgcagg agctcggcgc
     8281 cggggtgaga ttgggttcga tcgacaagga ggaaatagca agagcggtcg atagggtgct
     8341 gaaagagcct gtgtacagag aaaacgcggt ccgtatttca aaaagcttta aagcttgcgg
     8401 cggtgtgaga gaagcaaagg aatttataga gtcgaaaggg tgacctcccc gccgtcagcc
     8461 tgtttttcgc acctcgcctg ggcgtttggg agcctttgcg agagcggccc cccgatgtgt
     8521 gagagcggat ttcgcaccgg ccgcaggcgg tttttgcgcg gtgaaatttt atgcccgtcg
     8581 acgcggatat tattttgagg gagagtctta caatgccttt ttcacaaaag agcctggact
     8641 ttttgtttga gaaccggctg catgacagca aggaatggtt tttagagcac aaaaacgagt
     8701 atgagacgct ggtgaaagcg ccgctggtgg agctttccgc cgcgctgggg ccggtgatgc
     8761 tggagctgga cccccagctc accaccgacc cggcggtggg caagacgatc agccgcatcc
     8821 gccgggacac ccgcttcact catgacaagt cgctctaccg cgacaacatg tggatcgtct
     8881 acaagcgcgg caaaatgtac ggccgcgagg tgccggggct ttatttcgag gtgttcagcg
     8941 acgggcactt cggctatggc tgcggctttt acgacgcctc caccgcctat atggagacgc
     9001 tgcgcgccca ggtgcgcggg caaaccccgg cctggaaaaa ggccgacaag gccctgcgcg
     9061 cccagcaggt ctttgcactg gagggcgagc ggtacaagcg cccgcgtttt cccgacgcgc
     9121 cggcggccca gcgcgactgg ctggagctgc ggggcatcag cttcaacgcc cagcggcacg
     9181 acccggggct gctcttttcc gaccggctgg cggcggagct ggcagaggat tttcgcctgc
     9241 tggcgccgct ctacggcttt ttgctggagg tggccgggca gcagcgcgca gaggcacttc
     9301 agcgcccctg ggagctggca tagaaaaaag caaggggcgg cgatttttgc aaatcgccgc
     9361 cccttgcttt ttctgttttt atttgcccag agagtccatc acgtttaaaa tctcgtcgat
     9421 ctgttgctcc atccgctcct cgctgcccga ggagaacgcc tccttgacgc agccctccag
     9481 atgggcgcgg atgatctcct tgttggcctt gcgcaaaatc gcctcggcgg ccatgatctg
     9541 gttggcgatg tcgatgcagt acttgtcgtc gtccaccatc cgcagcacgc cgtcgatctg
     9601 gccgcgcgcg gtctttaaaa ggcgggtgat ctgggtcttg tcggctttca cgggttggga
     9661 tccgtccttt ctggaaaaat cgggcggctt cggccgcgcc aggggcgcgc ggggccgccc
     9721 ccccccacgc aggggctcac tgcaccgata cgacctcgta gcccgcctcg gtgacggcgg
     9781 tgaccagctt ttcgtcggcc acgtcggcgg ccagggtcac cacggcctgc gccgcttcca
     9841 ggtccacggt cacggcggac acgccctcca cggcggccag cgccttttcc acatgggcct
     9901 tgcagttgtt gcacatcatg cctttgatct tcagggtctt gttcatcacg gacacttcct
     9961 tttctttttt gtcgttttct atctcagagg gggcggccgc cgcgatgggg cagccgctcc
    10021 cggaggacgg gagggggtgg cggaacctgg gcttgaacag cttcaaccgc agcgcgttgg
    10081 acaccacgca gaacgaggac aggctcatgg ccgccgcgcc gaacatgggg ttcagcttcc
    10141 agcccagcag ggtataaaac acgcccgcgg ccagcgggat gccgatggag ttgtaaaaga
    10201 acgcccagaa caggttctgc ttgatgttgc ggatgaccga gcgggagagc tggaaagcgg
    10261 tgaccgcgtc cagcaggtcg ctcttcatca gcaccacgtc ggcgctctcg atggcgatgt
    10321 cggtgccggc gccgatggcg acgcccacgt ccgcccgggc cagggcgggg gcgtcgttga
    10381 tgccgtcccc gatcatggcc acctttttgc ccgcctgctg cagggccgcg acctcccgct
    10441 ccttgtcttg gggcagcacc tccgccacca cccggtcgat gcccagctgg ccgcggatgg
    10501 cctcggcggt gcgcttgttg tcgccggtga gcatgaccac gtccacgccc agggccttga
    10561 actcctcgat ggcggcccgg ctggtgggct tcaccacgtc ggccaccgcg atcacgccca
    10621 gcggctcgcc gtcccgggcg aagaacagcg gcgttttacc ctggttcgcc agcgcgtccg
    10681 catcgacccc gaacctgtct gcggagagac cgttcgcctc catcatgcgc aggttgcccg
    10741 ccagcacggg ctttccgtcc accttgccgg tcacgccctg gccgggcacg gtggcgaagt
    10801 ccgacgctgc atccggctcc agcccctggg cgcccgcgcg ccgcaggatg gccgcggcca
    10861 agggatgctc gctgggccgc tcggccgcgg cggccacccg cagcagctcg cgggggtctg
    10921 cgcccggcgc gggcagcagg tcggtgacct cgggcttgcc ctcggtgacg gtgccggtct
    10981 tgtccagcac caccgtgttc acagtgtggg ccacctcaag gctctcggcg gatttgaaga
    11041 ggatgccgtt ctcggcgccc ttgccggtgc ccaccatgat ggcggtgggg gtggccaggc
    11101 ccagcgcgca ggggcagctg atgaccagca ccgcgatgcc gatggagagc gcgaaatcga
    11161 cggggtagcc cagcagcagc cacaccgccg cggcgaccac cgcgatggtg atgaccaccg
    11221 gcacaaagac gccgctcacc ttgtccgcca gcttggcgat gggggccttg ctggcgttgg
    11281 cgtcctccac cagctgcacg atctgggcca gcgtggtgtc gtcgcccact ttcagagcct
    11341 tgcatttcag gtaaccggac tggttgatgg aggccgagat gaccttgtcg ccggggccct
    11401 tttccaccgg caggctctcg ccggtgaggg cggattcatc cagggccgcg ttgccctcca
    11461 aaatcacgcc gtccaccggg atacggccgc caggccgcac caccacggtg tcgcccacaa
    11521 cgacctcctc caccggcacc tcgatctcct cgccgtcccg ctccaccacc gcggtcttgg
    11581 gggcgaggtc cagcagcttg gtgatggcgt cgctggtgcg ccccttggag cgggtctcca
    11641 gatatttgcc caccgtgatc agggtcagaa tggtgcccgc cgattcaaag tagaggtcca
    11701 tgtggtagtg gccgaccagc tccatatcgc cgtgacccag gccgtagccg atgccgtaga
    11761 tggccaccag gccgtagacc accgccgccg ccgagccgat ggcgatcagc gagtccatgt
    11821 tcggcgcgcc gcggaacagc gtcttgaagc cgacctcata gtatttgcgg ttcacgtaca
    11881 tgatgggcag ggtcagcagc agctgggtca tggcaaagga gaccgcgttc tcatgcccca
    11941 gcagaaaagc gggcagcggc agccccatca tgtgccccat cgagacgtac atcaggggca
    12001 cgagaaagca aaaagagacg atcagccgcc gcttcatgcc cgccagctcg gcggccatgg
    12061 caccgccggg ggcctctttg ggcgcggcgg cgcactttgc ccccgcgccg tggggctgct
    12121 gtgggctggc cccgtagccg ccggccttga ccgcgtcgca gatctgcgcg tcgctgagcg
    12181 cgtcgccgtc gtattccacc atcatggtgt tctgcagcag attgaccgcc accgacgaga
    12241 cgccgggcag cttgcgcacg cttttttcca cgtgggcgct gcaggcggag caggtcatgc
    12301 cggtcacgtc gaatttttgt ttcattccca tcctatcctt tctctgttgg ggcgatcgcc
    12361 ggccgccggc cggcgggaaa gagggctgtt gaaaggacag ccgttcaaat accccacccc
    12421 tggggggtgt atatttagta tagttgccgc taatccgttt gtcaagggta gtttgcccga
    12481 aaaaatgttt tttaccgggc cgcggttctt ttgctgccgc tgggctcacg aaagaagagg
    12541 ggaggaggag aaagcggcct ggtgctgccg aagccgttgg ttccctgtga ggaaa
//
LOCUS       DBMDYA010000006        11622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1577_length_11622_cov_1.586668, whole genome shotgun sequence.
ACCESSION   DBMDYA010000006 DBMDYA010000000
VERSION     DBMDYA010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_11622_cov_1.586668"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..1228
                     /gene="hisC"
                     /locus_tag="ACZPFK_00370"
     CDS             158..1228
                     /gene="hisC"
                     /locus_tag="ACZPFK_00370"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR1404793.1"
                     /translation="MSRFLSERFRAVEPYVPGEQPQGRQYIKLNTNESPFPPSPRVTA
                     ALTPAEVEKLRLYSDPDARPLVQAIARRYGVGVQQVFVGNGSDEVLAFAFLAFGGGGR
                     AFRFPALSYGFYPVFARLFGVEFEAAPLRGDFTIDPADYIGCNKNVVLANPNAPTGLA
                     LGREEIRRIARSNPERVVLVDEAYVDFGGESAVPLLGECENLLVVQTFSKSRSLAGMR
                     VGFALGSPELIGDLQRMKFSFNPYNLDRLAILAGAAAMEDEAYFRACTEKVCATRRRV
                     TEALRDMSFTVLDSKANFVFAKPDGISGKALFTALRARGVLTRRWDEPLIRDYLRISI
                     GSDAEMDEFLQRTAEILREAGR"
     gene            1225..1809
                     /gene="hisB"
                     /locus_tag="ACZPFK_00375"
     CDS             1225..1809
                     /gene="hisB"
                     /locus_tag="ACZPFK_00375"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR1404794.1"
                     /translation="MRRAYIERKTAETDIRLELTLEGDGYENDTGCGFLDHMLDLFAK
                     HGGLGLRVKCRGDTRVDFHHTVEDIGIVLGEALRQALGDKRGIARYGSFLLPMDEALV
                     LTAIDLSGRTMCCCELDLPSQKVGEFDTELAEEFFLGFCRGAQVTLHLRQLAGKNTHH
                     ILEAAFKGFGRALAMAAAPDPRHPGELPSTKGVL"
     gene            1925..2536
                     /gene="hisH"
                     /locus_tag="ACZPFK_00380"
     CDS             1925..2536
                     /gene="hisH"
                     /locus_tag="ACZPFK_00380"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1404795.1"
                     /translation="MIAIIDYGVGNLFSLSSSLRHLGLETRITREARDLAAADRIILP
                     GVGAFADAAAKLRDTGLVDTICGQAAAGKPLLGICLGMQLLFEKSYEYGEHQGLGLVP
                     GVVAPLAEALPPGLKVPHIGWNQLDLLKKEDPLFRYSGQEDYVYYVHSYYATRCDANT
                     LAVSEYGLPVPGAVRAGSVVGVQFHPEKSGEAGLRLLRAFSEL"
     gene            2550..3287
                     /gene="hisA"
                     /locus_tag="ACZPFK_00385"
     CDS             2550..3287
                     /gene="hisA"
                     /locus_tag="ACZPFK_00385"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR1404796.1"
                     /translation="MELFPAIDLRAGKAVRLVQGDYDRMTVYSDRPAQVAERFRRQGA
                     RNLHLVDLDGAKEGAPVNFETVKEIVAQGGLFVEVGGGIRTERRIDRYLEIGVDRVIL
                     GTAAVADPGFLQRMVARYGPRIAVGVDARDGLVATHGWLQTTGLDSFAFCGRLRELGV
                     RTVIYTDIARDGGLAGTNLAAYRRLAGLKGLELVASGGISALEELKELAAMGVAGAIL
                     GKALYTGRLRLDEAVKLVQNPSGRGKA"
     gene            3332..3512
                     /locus_tag="ACZPFK_00390"
                     /pseudo
     CDS             3332..3512
                     /locus_tag="ACZPFK_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
     assembly_gap    3513..3522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3523..4041
                     /gene="hisF"
                     /locus_tag="ACZPFK_00395"
     CDS             <3523..4041
                     /gene="hisF"
                     /locus_tag="ACZPFK_00395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1404797.1"
                     /translation="GGIRSLEDFDRVLKCGADKVSVNSGAIADPSLIRRAAQRYGDQC
                     VVLSMDVKRVDGRFLLFARGGRENTGIDALDWAAQGVQNGAGEIVANSIDTDGVKGGF
                     DLELLDALAARVDVPIIASGGAGRMQDFADLFLGHPGVDAGLAASIFHRKEVEIGALK
                     RFLRARGVEVRL"
     gene            4081..4731
                     /gene="hisIE"
                     /locus_tag="ACZPFK_00400"
     CDS             4081..4731
                     /gene="hisIE"
                     /locus_tag="ACZPFK_00400"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR1404798.1"
                     /translation="MEKMSVELKFDEQGLIPAIVQDHYTREVLTLAYMNAESLALSIA
                     EKRTCFYSRSRRCLWRKGETSGNTQRIVSITADCDGDALVVEVVKAGPACHTGAESCF
                     FEPVFVSDELKPFCYEGLYQLIRGRKTDPKEGSYTTYLFEKGRDKILKKVGEECTEVI
                     IAGAKGDREETVYEIADLVYHVLVLMVESGITLKEVTAELEKRHVIDHKVKQERMQ"
     gene            4782..5600
                     /locus_tag="ACZPFK_00405"
     CDS             4782..5600
                     /locus_tag="ACZPFK_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1404799.1"
                     /translation="MQLKTSVHTHTTFCDGRDTPAAMAAAAAGLGLQVLGFSGHSLVR
                     REGFGIPPDRLPAYRAEIGRLRDAYAGRLQILCGLEVDTEAPQMELDGLDYLIGSAHA
                     VRDPKGRWWTVDATPELFARGVKEGFGGDALALAEAYYDQLCEYVLALRPTLVGHFDL
                     IRKFNAGDRFFEERGARYRALASAALARLLDAGLVLEVNTGAISRGWRQDPYPADFLL
                     RQALQAGGRVAVTADAHAAKDLTFYFDEALDLLRRLGFEQVLELTAAGWKERAL"
     gene            complement(5698..6873)
                     /locus_tag="ACZPFK_00410"
     CDS             complement(5698..6873)
                     /locus_tag="ACZPFK_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HIR1404800.1"
                     /translation="MSVRRELVASKKVSNISVRLINEQRILQQLYWNGGQTQLTLRNE
                     LRLSSPTITQALQAFKEAGLVLEGPEAASSGGRKPRLITFNYSAFHAVGVEIRRHHIA
                     VVVTDLQGRVVCEHTEPLSFENAPAYWRAINRSIHQLADAAPGVRRVLGVGLAFPGEL
                     SLDGSAIERAVVLGLRNEPLDDIGQYFDFDVAIENAANTAGFGAVWRAHGLEDAVYLI
                     VTDNGIAGSLIVGSQIFQGTNARRKVGAFGHMVLVPDGKPCFCGGRGCWSAYCALSNL
                     FARSDGDLDQFFQRVREGDPLYGQQWDSYLTYFAQALGNIRLALDMDIIIGGKIARYL
                     KEYLPLLEKKVVSHPSLQGDGNFLKVDDIEGNSLAIGAALIFVDRLLSGKLPRFPLP"
     gene            complement(6996..7292)
                     /locus_tag="ACZPFK_00415"
     CDS             complement(6996..7292)
                     /locus_tag="ACZPFK_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404801.1"
                     /translation="MSIFEVLMIVCFGFAWPVNVYNSLRTGSTKGKNLLFLSMIVLAY
                     VFGILHKLTFSRDPALWFYLFNAAMVSADVALYFRNRRREIADGRARGYTQVLC"
     gene            complement(7527..9224)
                     /locus_tag="ACZPFK_00420"
     CDS             complement(7527..9224)
                     /locus_tag="ACZPFK_00420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1404802.1"
                     /translation="MVDLKAKPFYLDDKACAWVRGTLAGMTELEKIGQLFCEILWDRP
                     GCDPEDLFSEITPGGVMYRPFSGARMNQVSRLLQDKAKVPLLIACNLERGGSGGNGGL
                     TDGTYVASPMGAAATGDPLQEYHLGLVAAREGAAAGINWTFEPIVDLDCNPENPITNV
                     RTFGSDPDRVIAMAKAYMDACRENGIATTIKHFPGDGVDYRDQHLMASVNSLSAEEWY
                     ASYGKVYKTLIDYGAPTLMSAHILQPALAREVNPGIADGDILPGSLSRELNQGILRDK
                     FGFNGLIVTDATQMVGFTCSMPRSRAVPTAIEHGADMFLFTINQKEDVGYMVQGLRDG
                     LLSRARLDEAVTRILALKASLGLHCKQPLVPPASALDILGCAEHRAWAAELADKCVTL
                     VKDREGLLPLSPAKTPRVKLIAVTNEKVKPGDVLPEVKLLRQLLEREGFQVSYFKDSA
                     YPGAGLSLADYRKETDLLIYYANMKVSSNQTTIRITWDDFLGEDSPKYVCDVPTLFLS
                     FSNPYHLTDVPMVRTYINAYTSNEVTVRAVVEKLMGRSAFQGQSPVDPFCGLWDAAR"
     gene            complement(9274..10263)
                     /locus_tag="ACZPFK_00425"
     CDS             complement(9274..10263)
                     /locus_tag="ACZPFK_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404803.1"
                     /translation="MNTATKKKFSKDDILVFFLKNRSLLLVLIFSILLSLSTSTFLTG
                     NNLMSLARQISANCIIGVGYTLLLASDSVDLSAGYMMCMIGIISGLLSQVEGMPFPVI
                     LLVCIVVGAVCGSWNAFIANKFKLPPFLVTLAMQQIFKGTLMLLSNGSPIGNLNEGII
                     FMGQGYLGPIPFPVILMVCFIAVGAVIFSRTQFGRDVIAIGGNPEAARVSGISVAKTR
                     ILVSALLGVTIAVTALVSCGRVASAQTTLGGDTVMDIIAAVVIGGTPMGGGSGTVVGT
                     LFGCLVIGLISNGLNLLRVSSFWQMVSKGVIILVAVILDVYSEKIYERMRNKD"
     gene            complement(10260..>10469)
                     /locus_tag="ACZPFK_00430"
     CDS             complement(10260..>10469)
                     /locus_tag="ACZPFK_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404804.1"
                     /translation="CNQLAKEGMAIILISSDLPEVMGMSDRLMVVREGRIVGRHGRGE
                     ASAEQLMAEMFGLSSTAQNREGSSK"
     assembly_gap    10470..10479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10480..>11622)
                     /locus_tag="ACZPFK_00435"
     CDS             complement(<10480..>11622)
                     /locus_tag="ACZPFK_00435"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1404805.1"
                     /translation="MLLAGKPYAPKGPHDALQQGISMIHQELRLVPSMTVADNVWIGR
                     VKNFSSGGLYFPERCVEATRKLFEEYGIELNPKERVANLSVAQMQMVEIARAVSYDSK
                     IVIMDEPTSALSDKEVEVLYKIIRRLSAAGKSIIFISHKLEEVFEIADRVTIFRDGRK
                     ISTQRVADITMSDLIDGIAGRQVKDLYPKLDVPRGEKVLEVRGIGKAGVFSDISFDVH
                     AGEIVGFCGLVGAGRTEIMNGIYGIDPIDSGEILLEGRPAVSRTPRQALNHGIAMVTE
                     DRLRRGVIVKMNVKLNLTLAYFYKICKGSFIDKRREARDAADMIGALRVKTAGPEMPI
                     WSLSGGNQQKVMVGRALLTKPKVLILDEPTRGVDVGAKSEIYQYCNQ"
BASE COUNT         2034 a         3508 c         4108 g         1952 t
ORIGIN      
        1 ggggggtgga gtttgcggaa aaagaggggc tggccgccca cgggcgcagc gtggcgatcc
       61 gctttgagga cgaaaagggc tgagcgggcg cggcccgcag agcaaagagg gccgcgccgg
      121 cacagacgcc gggccggccg atggggagga attgggattg agccgctttt tgagtgagag
      181 attcagggcc gtcgagccct atgtgcccgg cgagcagccg caggggcggc agtacatcaa
      241 gctgaacacc aacgagagcc cgtttccgcc ctcgccgcgg gtgaccgcag ccctcacccc
      301 ggcagaggtg gaaaagctgc ggctctactc cgacccggat gcccggccgc tggtgcaggc
      361 catcgcgcgg cggtacggcg tgggcgtgca gcaggtcttt gtgggcaacg gcagcgacga
      421 ggtgctggct tttgcttttc tggcctttgg cggcggcggg cgggctttcc gcttcccagc
      481 gctgagctac gggttctacc cggtctttgc gcggctgttt ggggtggagt tcgaggcggc
      541 gccgctgcgg ggggatttca ccatcgatcc ggcggattat atcgggtgca acaaaaacgt
      601 ggtgctggcc aaccccaacg cgcccaccgg cctggcgctg gggcgggagg agatccggcg
      661 catcgcgcgc tcgaacccgg agcgcgtggt gctggtggac gaggcctatg tggactttgg
      721 cggcgagagc gccgtgccgc tgctggggga gtgcgaaaac ctgctggtcg tgcagacctt
      781 ttccaaatcc cgcagcctgg cgggcatgcg ggtcggtttt gcgctgggca gccccgagct
      841 gatcggcgat ctgcagcgga tgaagttcag cttcaacccc tataacctgg accggctggc
      901 gattttggcg ggcgccgcgg cgatggagga cgaggcgtac ttccgcgcct gcaccgaaaa
      961 ggtctgcgcc acccgccgcc gggtgaccga ggcgctgcgg gacatgagct tcacggtgct
     1021 ggacagcaag gcgaactttg tctttgcaaa gcccgacggc atcagcggaa aagcgctctt
     1081 cactgccctg cgggcccgcg gcgtgctgac gcgccgctgg gacgagccgc tgatccggga
     1141 ctatctgcgc atctccatcg gcagcgacgc ggagatggac gaatttttgc agcggacggc
     1201 ggagattttg agggaggcgg gcagatgagg cgcgcgtata tcgagagaaa aacggcggag
     1261 accgacatcc ggctggagct gacgctggag ggcgacggct atgagaacga caccggctgc
     1321 ggttttttgg accatatgct ggacctgttt gcgaagcacg gcggcctggg cctgcgggtg
     1381 aagtgccggg gcgatacgcg ggtggacttt caccatacgg tggaggacat cggcatcgtg
     1441 ctgggcgagg ccctgcggca ggcgctgggg gacaagcggg gcatcgcccg gtacggcagc
     1501 tttctgctgc ccatggacga ggcgctggtg ctgacggcga tcgacctgtc ggggcgcacg
     1561 atgtgctgct gcgagctcga cctgcccagc caaaaggtgg gcgagttcga caccgagctg
     1621 gcggaggagt tctttctcgg cttttgccgg ggcgcgcagg tgacgctgca tctgaggcag
     1681 ctggcgggca aaaacaccca ccacatcctg gaggctgcgt tcaagggctt tgggcgcgcg
     1741 ctggccatgg cggcggcgcc cgacccgcgc cacccgggcg agctgcccag caccaagggc
     1801 gttttgtgac ggcgcggccc gcagaagagt tctgcccggc ccgcacgccg ggcgaaaaag
     1861 ggcccttgcg gcaaaaggca ctgtcggcat ggggaaaaca agaggaagat acggggggag
     1921 acaaatgatc gccatcattg attacggggt gggcaacctg ttcagcctgt cctccagcct
     1981 gcgccatctg gggctggaaa ccaggatcac ccgcgaggcg cgggacctgg ccgcagcaga
     2041 ccgcatcatc ctgccggggg tgggcgcgtt tgccgacgcg gcggcaaagc tgcgggacac
     2101 cggcctggtg gataccatct gcgggcaggc ggcggcgggc aagccgctgc tgggcatctg
     2161 tctgggcatg cagctgctgt ttgaaaaaag ctatgagtac ggcgagcatc aggggctggg
     2221 cctggtgccg ggcgtggtag ccccgctggc agaggcgctg ccccccgggc tgaaagtgcc
     2281 ccacatcggc tggaaccagc tggacctcct gaaaaaggag gacccgctgt tccgctattc
     2341 cgggcaggag gactacgtct attacgtcca cagctattac gccacccgct gtgacgccaa
     2401 caccctggcg gtgagcgagt acggcctgcc ggtccccggc gcggtgcgcg cgggcagcgt
     2461 ggtgggggtc cagttccacc cggaaaaaag cggggaggcg gggctgcggc tgctgcgggc
     2521 gttcagcgaa ctgtaaaggg ggcggtgaga tggagctttt tccggcgatc gacctgcggg
     2581 ccggcaaagc ggtgcggctg gtacaggggg attacgaccg catgacggtg tacagcgacc
     2641 ggcccgccca ggtggccgag cgctttcggc ggcagggagc gcgcaacctg catctggtgg
     2701 acctggacgg ggcgaaagag ggcgcgccgg tcaattttga gacggtgaaa gagatcgtgg
     2761 cccagggcgg gctgtttgtg gaggtgggcg gcggcatccg caccgagcgg cggatcgacc
     2821 gctacctgga gatcggggtg gatcgggtga tcctgggcac cgcggcggtg gcggaccccg
     2881 gctttttgca gcgcatggtg gcgcgttacg gcccgcgcat cgccgtgggg gtggatgccc
     2941 gggacggcct ggtggccacc cacggctggc tgcagaccac cgggctggac agctttgcct
     3001 tttgcggccg cctgcgcgag ctgggggtgc gcaccgtcat ctacaccgac atcgcccggg
     3061 acggcggcct ggccggcacc aatctggccg cctaccggcg gctggcgggg ttgaaagggc
     3121 tggagctcgt ggccagcggc ggcatctcgg cgctggagga gctgaaagag ctggcggcta
     3181 tgggcgtggc cggggcgatt ctgggcaagg cgctctacac cgggcggctg cggctggacg
     3241 aggcggtgaa gctggtgcaa aatccatcgg ggaggggtaa agcatgatca caaaacgcat
     3301 catcccatgc ctggacgtgc gggacggccg ggtggtcaag ggggtcaact tcgagggcgt
     3361 gcaggacgtg gccgacccgg tggaaatggc ccgcttttac aaccaggcgg gcgcggacga
     3421 gctggtcttt tacgatatca ccgccagcgc cgaggggcgc ggcatcttta ccgaggtgct
     3481 gcgccgggtg gcgggcgaga tcttcatccc gcnnnnnnnn nngggggcat ccgctcgctg
     3541 gaggatttcg accgggtgct caagtgcggg gcggacaagg tgagcgtcaa cagcggcgcc
     3601 atcgccgacc cgtcgctgat ccggcgcgcg gcccagcggt acggcgacca gtgtgtggtg
     3661 ctgagcatgg atgtgaagcg ggtggacggc cgttttctgc tgtttgcccg cggcggccgg
     3721 gaaaacaccg gcatcgacgc gctggactgg gccgcccagg gtgtgcaaaa cggcgccggc
     3781 gagatcgtgg ccaacagcat cgacaccgac ggggtcaagg gcggctttga cctggagctg
     3841 ctggacgcgc tggcggcgcg ggtggacgtg cccatcatcg cctcgggggg cgccggccgc
     3901 atgcaggact ttgccgacct ctttttgggc cacccggggg tggatgcggg cctggcggcc
     3961 agcatctttc accgcaaaga ggtggagatc ggggcgctca agcgcttttt gcgcgccagg
     4021 ggcgtggagg tccggctgtg agagccgcgc gccgggaaca gccgcaatac cgcagaggga
     4081 gtggaaaaga tgtctgttga actgaaattt gacgagcagg gcctgatccc ggccatcgtc
     4141 caggaccact ataccaggga ggtgctgacg ctggcctata tgaacgccga gagcctggcc
     4201 ctcagcatcg ccgaaaagcg cacctgcttt tacagccgca gccgccgctg cctttggcgc
     4261 aagggggaga ccagcggcaa cacgcagcgc atcgtctcca tcaccgcgga ttgcgacggc
     4321 gacgcgctgg tggtggaggt ggtcaaggcg ggcccggcct gccacaccgg cgcggagagc
     4381 tgtttttttg agccggtctt tgtcagcgac gagctcaagc cgttctgcta cgaggggctc
     4441 taccagctga tccgcggccg caagaccgac cccaaggagg gcagctacac cacctacctg
     4501 tttgaaaagg ggcgggacaa gatcctcaaa aaagtggggg aggagtgcac cgaggtaatc
     4561 atcgccgggg cgaaaggcga ccgggaggag acggtttacg agatcgccga tctggtgtat
     4621 catgtactgg tgctgatggt cgagagcggc atcaccctga aagaggtgac tgcggagctg
     4681 gaaaaacggc atgtgatcga ccacaaggtg aaacaggagc ggatgcaata gccgggggac
     4741 agccgccccg gcccgcgccg gaagatacgg gggaaaagca gatgcagctg aagaccagtg
     4801 tccacaccca caccacgttc tgcgacggcc gcgacacccc ggcggcgatg gcggcggccg
     4861 cggcggggct ggggctgcag gtgctcggct tttccggcca cagcttggtg cggcgggagg
     4921 gcttcggcat cccgccggac cgcctgcccg cctaccgcgc cgagatcggg cggctgcggg
     4981 acgcctatgc gggcaggctg cagatcctct gcgggctgga ggtggacacc gaggcgccgc
     5041 agatggagct ggacggtttg gattatttga tcggctccgc acacgcggtg cgggacccga
     5101 aagggcgctg gtggacggtg gacgccacgc cggagctctt tgcccgaggc gtgaaagagg
     5161 ggttcggcgg cgacgcgctg gccctggcgg aggcctatta cgaccagctc tgtgagtatg
     5221 tgctggcgct gcgccccacc ctggtggggc actttgacct gatccgcaag ttcaacgccg
     5281 gggaccgctt ttttgaggag cggggcgcgc gataccgcgc cctggcctcg gcggcgctgg
     5341 cccggctgct ggacgccggg ctggtgctgg aggtgaacac cggggccatc agtcgcggct
     5401 ggcggcagga cccctatccc gcggatttcc tgctgcggca ggctctgcag gcgggcggcc
     5461 gggtggccgt cacggcggac gcccacgcgg caaaggacct gaccttttat ttcgacgagg
     5521 cgctggacct gctgcgcagg ctcggctttg agcaggtgct ggagctgacg gcggcggggt
     5581 ggaaagagcg ggcgctctga ggaggcctgg gacggagaat aaaaaagaag agggaaaacg
     5641 cagtcggcgc gttttccctc ttcttttttt gaccggccgc cgcccggcgc gggtggatca
     5701 gggcagcggg aaccggggca gcttgcccga gagcaggcgg tcgacaaaga tcagcgccgc
     5761 gccgatggcc agcgagttgc cctcgatgtc gtccaccttt aaaaagttgc cgtccccctg
     5821 caaagagggg tgggagacga cctttttttc cagcaggggc agatattcct tgagatagcg
     5881 ggcgatcttg ccgccgatga tgatatccat atccagcgcc aggcggatat tgcccagcgc
     5941 ctgcgcaaag taggtcagat aagagtccca ttgctggccg tacagagggt cgccctcgcg
     6001 cacccgctga aaaaactgat ccaggtcccc gtccgagcgg gcgaagagat tggacagcgc
     6061 gcagtaggcg gaccagcagc cgcggccgcc gcaaaagcag ggcttgccgt cgggcaccag
     6121 caccatatgg ccgaacgccc ctaccttgcg ccgggcgttg gtgccctgaa agatctggct
     6181 gcccacaatc aacgagccgg cgatgccgtt gtcggtgacg atgaggtaga cggcgtcctc
     6241 cagcccgtgg gcgcgccaga ccgcgccgaa gcccgccgtg ttggcggcgt tttcgatggc
     6301 cacgtcaaag tcgaaatact ggccgatgtc gtccagcggc tcgttgcgca ggcccagcac
     6361 cacagcgcgc tcgatggcgc tgccgtccag cgagagctcg cccgggaagg ccagccccac
     6421 ccccagcacc cggcgcaccc cgggcgcggc gtccgccagc tggtggatgc tgcggttgat
     6481 ggcccgccag taggcggggg cgttttcaaa ggagagcggc tcggtgtgct cgcacaccac
     6541 ccggccctgc aggtcggtga cgaccaccgc gatgtgatgc cggcggatct ccacccccac
     6601 ggcgtgaaaa gcgctgtagt tgaacgtgat caggcggggc ttgcggccgc cggaggaggc
     6661 ggcctcgggg ccctccagca ccagccctgc ctctttgaac gcctgcaggg cctgggtgat
     6721 ggtgggcgag ctcagcctca gctcgttgcg cagggtgagc tgtgtctgcc ctccgttcca
     6781 gtagagctgc tgcaggatgc gctgctcatt gatcagccgc accgaaatat tactcacctt
     6841 tttgctggcg accagctccc ggcgcacgga cataccgcac ccttctttcg cagaaaaaat
     6901 ttcataaaat gatttttaga aaactggtta aaaatgtaat tttaattatt gtagcaaacc
     6961 ggcccggcgt ttgcaaccgc aggccgccgc gccgctcaac agagaacctg tgtgtagccg
     7021 cgcgccctgc cgtctgcgat ctcccgcctg cggttgcgga aatagagggc cacgtccgcc
     7081 gacaccatgg cggcgttgaa cagatagaac cagagcgccg ggtcgcggga aaaggtcagc
     7141 ttgtgcagga tgccgaagac gtaggccagc acgatcatgg agagaaagag caggtttttg
     7201 ccctttgtgg aacccgtgcg caggctgttg tagacgttga cgggccaggc aaagccgaag
     7261 cagacgatca tcaggacttc aaaaatcgac atgaggttac ctccagaaac cgtttgggga
     7321 accgccgggg cggcggcgcc gcccgcccct ccgagagaag cgcaagccgg aaagctcccc
     7381 gagggcggag agggacgcgc gcagcggccc cgcaaggcaa aagcgcgggg cctccgcggc
     7441 ccccgctcag cgaaaaaggg aggcctgcgg cttgccgtgc cccggggaga gaggcgagcg
     7501 gaaaagaagc gcgcagggga cggcgctcac cgcgccgcgt cccacaggcc gcagaacggg
     7561 tccaccgggc tttgcccctg aaaggcggac cggcccatca gcttttccac caccgcccgc
     7621 acggtgacct cgttagaggt gtaggcgttg atataggtgc gcaccatggg cacgtcggtc
     7681 aggtgatagg ggttggagaa agagagaaaa agggtgggga cgtcacagac gtatttgggc
     7741 gagtcctccc ccagaaaatc gtcccaggtg atgcggatcg tggtctggtt gctgctcacc
     7801 ttcatgttgg cgtaatagat cagcaggtcg gtctccttgc ggtagtcggc cagcgaaagc
     7861 ccggcgcccg ggtaggcgct gtccttgaaa tagctgacct ggaagccctc ccgctccagc
     7921 agctgtcgga gcagcttcac ctcgggcaag acgtcgccgg gcttcacctt ttcgttggtg
     7981 accgcgatca gcttgacccg gggcgttttg gcgggggaga ggggaagcag gccctcccgg
     8041 tccttgacca gggtgacgca tttgtccgcc agctcggcgg cccaggcccg atgctccgca
     8101 cagcccagga tatccagcgc ggaggcgggc ggcaccaggg gctgtttgca gtgcaggccg
     8161 agagaggcct tgagggcgag aatgcgggtc accgcctcgt cgaggcgggc gcgggagaga
     8221 agcccgtccc gcagcccctg gaccatatag cccacgtcct ctttctggtt gatggtgaac
     8281 aggaacatgt ccgccccgtg ctcgatggcg gtgggcaccg cccggctgcg gggcatggag
     8341 caggtaaagc ccaccatctg ggtggcgtcg gtgacgatca ggccgttaaa gccgaatttg
     8401 tcccgcaaaa tgccctggtt gagctcgcgg gagagggagc cgggcaggat gtcgccgtcc
     8461 gcgatgccgg ggttcacctc ccgcgccagc gcgggctgca ggatatgggc ggacatcagg
     8521 gtgggagcgc cgtagtcgat cagcgttttg tacaccttgc cgtagctggc gtaccactcc
     8581 tccgccgaca ggctgttgac gctggccatc agatgctggt cccggtagtc caccccgtcg
     8641 ccggggaagt gcttgatggt ggtggcgatg ccgttttccc ggcaggcgtc catataggcc
     8701 tttgccatgg cgatcaccct gtccgggtcg ctgccgaacg tgcgcacgtt ggtgatgggg
     8761 ttttccgggt tgcagtccag gtcgacgatg ggctcaaagg tccagttgat gcccgccgcc
     8821 gcgccctccc gggccgccac cagccccagg tggtactcct gcagcgggtc cccggtggcg
     8881 gcggccccca tggggctggc cacataggtg ccgtcggtga ggccgccgtt gccgccgctg
     8941 ccgccccgct ccaggttgca ggcgatgagc aggggcacct ttgccttgtc ctgcagcagg
     9001 cgggagacct ggttcatccg cgcgccgctg aaaggccggt acatcacccc gcccggggtg
     9061 atctcggaga acaggtcttc cgggtcgcag cctgggcggt cccagagaat ctcacagaat
     9121 aactggccga tcttttccag ctcggtcatg ccggcgaggg tcccgcgcac ccaggcgcag
     9181 gccttgtcgt ccagataaaa gggcttggct ttcaaatcca ccatgccggt tcacaccttt
     9241 ccgtttgggg ttgttttgcg ccggtggacc ggctcagtcc ttgttgcgca tgcgctcata
     9301 gatcttttca ctgtagacgt ccaggatgac ggccaccagg atgatgacgc ctttcgacac
     9361 catctgccag aagctggaca cccgcagcag gttgaggccg ttgctgatga ggccgatgac
     9421 aaggcagccg aacagggtgc ccaccacggt gccgctgccg ccgcccatgg gggtgccgcc
     9481 gatgaccacg gcggcgatga tgtccatgac cgtgtcgccg cccagcgtgg tctgggccga
     9541 ggcgacgcgg ccgcaggaca ccagcgccgt gacggcgatg gtgacgccca gcagcgccga
     9601 gacgaggatg cgggtctttg ccacgctgat gccggagacg cgggcggcct cagggttgcc
     9661 gccgatggcg atgacgtcgc ggccgaactg ggtgcggctg aagatgacgg cccccacggc
     9721 gataaagcac accatcagga tgaccgggaa ggggatgggc cccagatagc cctggcccat
     9781 aaagatgatg ccctcgttca ggttgccgat gggcgagccg ttgctcagca gcatcagggt
     9841 gcccttgaag atctgctgca tggcgagcgt caccagaaag ggcggcagct tgaatttgtt
     9901 ggcgataaag gcgttccagg agccgcagac cgcgcccacg acgatgcaga cgagcaggat
     9961 gacggggaac ggcatcccct cgacctggga gagcagcccc gagatgatgc cgatcatgca
    10021 catcatatag cccgccgaga gatccacact gtcggaggcc agaagcagcg tatagcccac
    10081 gccgatgatg cagttggcgc tgatctggcg cgccaggctc atcaggttgt tgccggtgag
    10141 aaaggtgctg gtgctgagcg agagcaggat gctgaagatg agcaccagca gcagcgagcg
    10201 gtttttgaga aaaaagacca ggatatcgtc tttggaaaat tttttctttg tcgcggtatt
    10261 catttggatg agccctccct attctgcgca gtggatgaaa ggccgaacat ctcggccatc
    10321 agctgttctg ccgacgcctc gccgcggccg tggcggccca cgatgcggcc ctcgcgcacc
    10381 accatcaggc ggtcgctcat gcccatgacc tcgggcagat cgctggagat gaggatgatg
    10441 gccatgccct cttttgccag ctggttgcan nnnnnnnnnc tggttgcagt actgatagat
    10501 ctcgctcttc gcgcccacgt ccacgccgcg ggtgggctcg tccaggatga gcaccttggg
    10561 cttggtcaac agggcgcggc ccaccatcac cttttgctgg ttgccccccg acagcgacca
    10621 gatcggcatc tcggggcccg cggtcttgac ccgcagggcg ccgatcatgt cggcggcgtc
    10681 ccgcgcctcc ctgcgcttgt cgataaagga ccctttgcag atcttataga aataggcgag
    10741 ggtcaggttg agcttgacgt tcattttgac gatgacgccg cgccgcagcc ggtcctcggt
    10801 gaccatggcg atgccgtggt tgagcgcctg gcgcggggta cggctcacgg cgggccgccc
    10861 ctccagcagg atctcgccgc tgtcgatggg gtcgatgccg tagatgccgt tcatgatctc
    10921 ggtgcgcccg gcgcccacca ggccgcaaaa gccgacgatc tcgcccgcgt ggacgtcaaa
    10981 gctgatgtcg ctgaacaccc cggccttgcc gatgccgcgc acctccagca ccttttcgcc
    11041 gcggggcacg tccagcttgg ggtacaggtc tttcacctgc cgcccggcga tgccgtcgat
    11101 caggtcggac atggtgatgt ccgccaccct ctgggtgctg atcttgcggc cgtcgcggaa
    11161 gatcgtcacc cggtcggcga tctcaaagac ctcctccagc ttgtgggaga tgaagatgat
    11221 cgatttgccg gccgcagaga gccggcggat gatcttgtag agcacctcca cctccttgtc
    11281 cgacagggcg ctggtgggct cgtccatgat gacgatcttg gagtcgtaag agaccgcgcg
    11341 cgcgatctcc accatctgca tctgggccac gctcaggttg gccacccgct ctttggggtt
    11401 gagctcgatg ccgtattcct cgaacagctt gcgggtcgcc tccacacacc gctcggggaa
    11461 atagagcccc ccactggaaa agtttttgac ccggccgatc cagacgttgt cggccaccgt
    11521 catgctgggc accaggcgaa gctcctgatg aatcatcgag atgccctgtt gaagggcgtc
    11581 gtggggcccc ttgggggcgt agggcttgcc cgccagcagc at
//
LOCUS       DBMDYA010000007        11487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1597_length_11487_cov_1.488319, whole genome shotgun sequence.
ACCESSION   DBMDYA010000007 DBMDYA010000000
VERSION     DBMDYA010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_11487_cov_1.488319"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            4..174
                     /locus_tag="ACZPFK_00440"
     CDS             4..174
                     /locus_tag="ACZPFK_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1404806.1"
                     /translation="MATLYAAQMLRWDDRVGSVEAGKLADLVAVDADPVADIDEMTRV
                     TFVMKDGKIYKQ"
     gene            526..651
                     /locus_tag="ACZPFK_00445"
     CDS             526..651
                     /locus_tag="ACZPFK_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404807.1"
                     /translation="MEQDKKIVEKLIAEMEALTEFQKEAVSWLVCHLHLAELLCA"
     gene            1229..1366
                     /locus_tag="ACZPFK_00450"
     CDS             1229..1366
                     /locus_tag="ACZPFK_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404808.1"
                     /translation="MKTLYELCSEMVRLYREQQPDEAAAQLPALRAAHERVCGAIAEC
                     F"
     gene            1399..5139
                     /locus_tag="ACZPFK_00455"
     CDS             1399..5139
                     /locus_tag="ACZPFK_00455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1404809.1"
                     /translation="MEKRDTLLLVDDVEVNRAILRGIFEREYNLLEAENGDQALVLIE
                     QYHGRIAAILLDLVMPVRDGYQVLEALGRRGLLAELPVIVITAEDSAASEVRAFDLGA
                     SDIIMKPFEPHVVRRRVQNVVELNLHKLGQQERIEEQAAKLRESNAVMVDALSSIIEY
                     RSVETGQHIQRIRMFTRVLLEEVARCHPEYGLDARKIDMISSASSMHDIGKIAIPDAI
                     LNKPGRLTDEEFEIMKTHSQRGCEMLANLDRMGDQEYLQYAYNICRYHHERWDGRGYP
                     DGLKGDAIPVCAQVVGIADCYDALTTDRVYRGALPAEQAFNMILNGECGSFSPRLLEC
                     FKSVQGAFAQLSQEYADGTRRTHPAAVPRRAPEPSFGEGALDSLQLSQMKYFTLLRYA
                     DATVMEADLTTGLYHLVYLASGDFELLRAGGCFEEAFRGFVKNAVHPDDQALALELLG
                     DYLDEFFREGLMRRSRSYRVFSHATQAYRRCEATMLRIDTGHPRQRKVLIVWKELDPG
                     AEAAPRFADGAALGALLGGVQVCRNDRWFTMERMNGSLGALLGYSEEEIRERFENRYL
                     ELIYPPDRDDALRQTRRQLQAGDAIELEYRLQAKDGRLVWTLDKGLRKTGPDGGEYLV
                     RVLLDITQSKQAQEELRRTIERHKLIMEQSNDIIFEWDIERDEVLYSANWEKKFGYEP
                     IREHASARIPQVSHVHPEDLPQFTRLISDMAGGVPYEEIEFRVADATGRYRWSKIRAA
                     AQFNEAGKPYKAVGVIADIDEERRATEALRERAERDELTQLYNKSAGRGLIEDYFAKR
                     PAQEGAALLIVDLDNFKQINDTYGHLFGDVVLREFSAELRRLFRGEDILSRIGGDEFL
                     IFMRGVPDPEAVERRVDRVVESFRQMFRHDVLQGNPSCSIGISFCPADGQDFETLFRH
                     ADLALYGAKSKGKNCWRRFDAATMSDSFGLGTRQPVASTRIESGDLPETAVQGLVEQA
                     FLELQRTDDLGAAIHAILGMIGKKFGVSRAYVLEDSRDGRITSNTFEWCNDGIESQKT
                     LLQRIEYEKLGGRRAYLSLFNEEGVFYCPDVQLLAQQEIRALLMQQDIKSTLQCAIRD
                     ESGFRGFVGFDDCVIRRMWTREQIEALTFTSRLLSLFLLKRRAQEAAERAAGDLRTVL
                     DHQNAWVYVVERGSWKLRYANGKTRRLAPQAREGAVCYRALFGRSEPCAHCPVREGAT
                     AGGTEMYNGRLGVWALVNATAVQWEGTPCHLLTCQDITDYKK"
     gene            5631..8924
                     /locus_tag="ACZPFK_00460"
     CDS             5631..8924
                     /locus_tag="ACZPFK_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1404810.1"
                     /translation="MDEEKKQRVEAFAHEILYSYYTESDVELLIAHLAPEVVWLGAGR
                     NQRAEGREAVAAVFRAGREELFRCEISGERYVTRSLGGGCWLCQGDSQVTSAPGTGKY
                     IQAHQRITFLFQEQGQRLLIAHIHHSMDYTGVADGELFPVKAGQAAYQKLQATLEEKD
                     RQIELMLTQMPGGMAICLPDEDFTVDWISSSLCKLLGYAGPEEFFAATGGACRAFIQP
                     EDYDEMARKVEGRFSDSDEYYAEYRVRLQDGGALWASDLGRRITDADGRTLIYCFISD
                     ITRRRERELEIERANREAARQARFLGQLYESIPCGILQFPPGPEHRIISLNPMVWRFY
                     GFGSEAEYRAAVSDPFDLVLEEERPRLQRLVDGLPLGGGTISYTRQVRRRDGREGWIS
                     VVLERLVNIDGVEVLQAIFTDITEIKALQAAQEREQLLENRVLRAAICTAYPMIMSLN
                     LTRGTYSCFVDQQGFYDGRRQGDFEEMAREAVANTYPSYREDFARFLSREEILRRFAA
                     GEQELYTELRQLGVDGAYHWNSVQLIQVENPVGDELMAIELVRVLDEQRAEKARQEQL
                     LRDALAAARAANSAKSDFLSRMSHDIRTPMNAIIGMSTIGQLKAGDPQAVRNCFAKID
                     TSSRFLLSLINDILDMSKIENGKMAIAEERFDFTAFFDELVSIIYPQAEKAGLEFEVH
                     ISAALERWYVGDELRLKQVLMNLLSNALKFTPRGGRIVVEAAEERRAAGKAVLRFDVR
                     DTGIGMSEEFLTRIFNPFEQEEESAARNNVGSGLGLSIVYNLVQLMGGEVQVHSRRGQ
                     GAAFAVRLPLGLLEDDAEEEQRRRARELMQGAPVLVADDDPAVGEQACLILGGIGARP
                     EWAASGAEAVEKVRCALARGEHYEIALIDWRMPGMDGVETTRRIRQLVGPETTIVIIS
                     AYDWSDIEQEARAAGADHFLSKPLFSSTLCHAVTHLENAAARGHSTVDRALAGRRVLL
                     VEDNELNREIARSLLELYGLEVDTAENGREAVERFAAREAGTYLAVLMDIRMPVMDGL
                     EATRAIRALPRGDAARVPILAMTANAFDEDRTQAYRAGMTGYLTKPLDIQVLLEALEK
                     LLPAPPEKP"
     gene            9521..9907
                     /locus_tag="ACZPFK_00465"
     CDS             9521..9907
                     /locus_tag="ACZPFK_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013231.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1404811.1"
                     /translation="MTYNSLLYLDLIAYTPDCTAGYLAETLHVAPSAITSKVRELERL
                     GLVTRTRSERDKRVYYLHINEQLRAEYRAYDQILYGALDELEARYTPRQVDLLCEMLD
                     AINRGFQQAKTARALPGGKEKKGKET"
     gene            9904..11229
                     /locus_tag="ACZPFK_00470"
     CDS             9904..11229
                     /locus_tag="ACZPFK_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1404812.1"
                     /translation="MNSLEKKITPGFLLRFTLPTVVMMVFNSFYTMVDGGFVSRFVGT
                     GALSAVNIVYPALNLVFALGIMLATGGSAVVAKLLGEGREEHARRSFTTLVVVALALG
                     AAMTAAGTLLAGPGVRMLGANDAVFAYCRDYMFTLSLFVPFAMLQLLFQYFFVAAARP
                     NLGLISTVAGGVANIVLDYVFIVPLKMGVTGAALATGLGFVIPAVVGLVFFACNRKGA
                     LHFARPRWEPRLLACACANGSSEMVSNLSVAVTTFLFNLLMMRALGEDGVAAITIVLY
                     SQFLFTAVFLGYVSGVAPLFSFNYGARDKKRLGQLFRLSLIFIAVCSLLAWALSLALS
                     QQVVSVFAKRGEPVFDLAVHGLFLFSAGFLFMGFNIFASGLFTALSNGRVSAILSFLR
                     TFVFIVAAILLLPRLLGVDGIWLSIPAAEALALLVSAWYMVRLKPVYGY"
BASE COUNT         2011 a         3564 c         3967 g         1945 t
ORIGIN      
        1 atgatggcca ccctctacgc ggcgcagatg ctgcgctggg acgaccgggt gggcagcgtc
       61 gaggcgggca agctggccga tctcgtggcg gtggacgccg accccgtcgc ggatatcgac
      121 gagatgacgc gggtcacctt tgtcatgaaa gacggcaaaa tttataagca gtgagcgctc
      181 cgcgcagagc gggcagggcc ctccccatcc ggggagggcc ctttttatct gccccgctcc
      241 cgattttgca cgagcggaaa ataaattttg cggcaggatg aatccatcca gcaggaggct
      301 ttttcgtccg ctataataaa agctgaggcg ggtggggtga aaccggcggc gcgcatgttt
      361 tacctgcccg ggcaagagga gacgggcggg cgaggaaatg aggggaacgc ggcgggctgt
      421 tttttcgctt ccgcttttgc cgcttgcgat ggcggggaaa gccgagtgct tcaaacaaat
      481 cgtttgagga cggcgcgaaa aaacactgga taggaggcaa agaaaatgga acaggacaaa
      541 aaaatcgttg aaaaactcat cgcggagatg gaagctttga cggagtttca aaaagaggcg
      601 gtctcgtggc tggtctgcca cctccacctg gcggagctgc tctgtgcgta gggagagccc
      661 ctggatgagg acatgtttga aaaggaggtc gccagggccg ttcgcacacg ggattatgca
      721 tattacgggc tgctgtttta ccagagggca agccgggagc agggccgcga aaacggtgaa
      781 aagcccacgg gggatccttg caccggccgg taaaagcagg cccggaccgg tcccgcgctg
      841 gtaacagggc ctgccccgcc ggcggcgggg agaagaaaaa cgcaaaatgt cacaataaca
      901 ccttaatcct tgccgctgat tgtggtataa tgagataaaa tatgcccggg cagggaattg
      961 gctttcacac aggcccgccg gccgggggat caaaggagaa aacgcgcgat ggaagagatg
     1021 cagcgcacaa ggctggaaag tgccgggatg gagatcgacc gcacggtgga acggttcagc
     1081 ggcaacgagg ggatctatgt gaaattcctg aaaaaatttc ccgacgatga gaacctggcg
     1141 cagctgtcgc aggccgttgc cgcgggggac gatgagacgg ccctgcgcgc ggcgcacacc
     1201 ctgaaaggcc tcttgcgcga acctgggcat gaaaacgctg tacgagctgt gcagcgagat
     1261 ggtgcgcctt taccgcgagc agcagcccga cgaggccgcc gcgcagctgc ccgccctgcg
     1321 ggcggctcac gagcgggtct gcggggcgat tgccgaatgt ttttgagcgc ggctgtgcgc
     1381 cgctgaaaga gtgaagctat ggaaaaaagg gataccctgc tgctcgtgga cgacgtagag
     1441 gtcaaccgcg ccatcctgcg cggcatcttc gagcgggagt acaatctgct ggaggcggag
     1501 aacggcgacc aggccctggt gctcatcgag cagtaccacg gcaggatcgc cgcaatcctg
     1561 ctggacctcg tcatgccggt gcgggacggc tatcaggtgc tggaggcatt gggccgccgg
     1621 gggctgctgg ccgagctgcc ggtcatcgtc atcacggcgg aggattcggc cgccagcgag
     1681 gtgcgcgcgt tcgacctggg cgcgtcggac atcatcatga agccgttcga gccccatgtg
     1741 gtgcgccgcc gcgtgcagaa cgtggtggag ctgaacctgc acaagctggg ccagcaggag
     1801 cgcatcgagg agcaggcggc caagctgcgc gagtccaacg ccgtcatggt ggacgcgctc
     1861 tcctccatca tcgagtaccg cagcgtcgag accggccagc acatccagcg catccgcatg
     1921 ttcacgcggg ttttgctgga ggaggtggcc cgctgccacc ccgagtacgg cctggacgcc
     1981 cgcaagatcg acatgatctc cagcgcctcc tcgatgcacg atatcggcaa gatcgccatc
     2041 cccgacgcga ttctcaacaa gccgggccgt ctcaccgacg aggagttcga gatcatgaag
     2101 acccactcgc agcgcggctg cgagatgctg gccaacctgg accgcatggg ggaccaggaa
     2161 tatctgcagt acgcctacaa catctgccgc tatcaccacg agcgctggga cggccgcggc
     2221 tatcccgacg ggctcaaggg cgacgccatc cccgtctgcg cccaggtggt gggcattgcc
     2281 gactgctacg acgccctcac caccgaccgg gtctaccggg gcgcgctgcc cgccgagcag
     2341 gcgttcaaca tgatcctcaa cggcgagtgc ggctcttttt cgccgcggct gctggagtgc
     2401 ttcaaaagcg tgcagggggc gtttgcccag ctgtcgcagg agtatgccga cggcacccgg
     2461 cgcacccatc ccgcggccgt gccgcggcgc gcgccggagc cctcgttcgg cgagggggcg
     2521 ctggattcgc tgcagctcag ccagatgaaa tatttcacgc tgctgcggta tgccgacgcc
     2581 accgtgatgg aggccgacct caccaccggc ctgtaccatc tggtctatct ggccagcggc
     2641 gattttgagc tgctgcgcgc gggcggctgc tttgaagagg cgttccgcgg ctttgtgaaa
     2701 aatgccgtcc accccgacga ccaggccctg gcgctggagc tgctggggga ttacctggac
     2761 gaatttttcc gtgaggggct gatgcggcgc agccgcagct accgcgtctt cagccatgcc
     2821 acccaggcct atcgccgctg tgaggccacc atgctgcgca tcgacaccgg ccacccgcgc
     2881 cagcgcaagg tgctcatcgt ctggaaagag ctggaccccg gcgccgaggc ggccccccgg
     2941 tttgccgacg gcgccgcgct gggcgcgctg ctgggcggcg tgcaggtgtg ccgcaacgac
     3001 cgctggttca ccatggagcg gatgaacggc agcctgggcg cgctgctcgg ctacagcgag
     3061 gaggagatcc gcgaaaggtt tgaaaaccgc tatctggagc tgatctaccc gcccgaccgg
     3121 gacgacgccc tgcgtcagac gcggcggcag ctgcaggcgg gcgacgccat cgagctcgag
     3181 taccgcctgc aggccaagga cggcaggctg gtgtggacgc tggacaaggg gctgcgcaag
     3241 acgggcccgg acggaggcga atatctggtg cgggtgctgc tggacatcac ccagtccaag
     3301 caggcgcagg aggagctgcg ccgcaccatc gagcggcaca agctcatcat ggagcagtcc
     3361 aacgacatca tcttcgagtg ggatattgag cgggacgagg tgctctattc cgccaattgg
     3421 gaaaagaagt tcggctatga gcccatccgg gaacatgcca gcgcccgcat cccgcaggtc
     3481 tcgcatgtcc accccgagga cctgccccag ttcacccggc tgatcagcga tatggcgggc
     3541 ggggtgccct atgaggagat cgagttccgg gtggcggacg ccaccgggcg ctaccgctgg
     3601 agcaaaatcc gcgctgcggc ccagttcaac gaggcgggca agccgtataa ggcggtgggg
     3661 gtgatcgccg atatcgacga ggagcggcgc gccaccgagg ccctgcggga gcgcgccgag
     3721 cgggacgagc tgacgcagct gtacaacaag agcgcggggc gcggcctgat cgaggactat
     3781 tttgcaaagc ggcccgcgca ggagggggcg gccctgctga tcgtggacct ggacaatttc
     3841 aaacagatca acgacaccta cggccacctg tttggggacg tggtgctgcg tgagttttcc
     3901 gccgagctgc gccgcctctt ccgcggcgag gacatcctct cccgcatcgg cggcgacgag
     3961 tttctcatct tcatgcgcgg cgtgccggac cccgaggccg tcgagcggcg ggtggaccgg
     4021 gtggtggaat cgttccggca gatgttccgc cacgacgtgc tgcagggcaa cccctcctgc
     4081 agcatcggca tctcgttctg ccccgcggac gggcaggatt ttgagaccct gttccggcac
     4141 gcggacctgg cgctctacgg ggcgaaatcc aagggcaaaa actgctggcg caggtttgac
     4201 gccgccacca tgagcgactc gttcgggctg gggacgcggc agccggtggc cagcacccgc
     4261 atcgagtcgg gggatttgcc ggagaccgcc gtgcagggcc tggtggagca ggcgtttctt
     4321 gagctgcagc gcacggacga cctgggggcc gcgatccatg cgattttggg catgatcggc
     4381 aaaaagttcg gggtcagccg cgcctatgtc ctcgaggaca gccgggacgg ccgcatcacc
     4441 agcaacacct ttgagtggtg caacgacggc attgagtcgc agaaaacgct gctgcagcgg
     4501 atcgagtacg aaaagctggg cggccgcagg gcctatctct cgctcttcaa cgaggagggg
     4561 gtgttctact gccccgacgt gcagctgctg gcccagcagg agatccgcgc cctgctgatg
     4621 cagcaggaca tcaagtccac actgcagtgc gccatccggg acgagagcgg ttttcggggc
     4681 tttgtgggct ttgacgactg cgtcatccgg cgcatgtgga cgcgggagca gatcgaggcg
     4741 ctcaccttta cctcccgcct gctgtccctc tttttgctca agcgccgcgc ccaggaggcg
     4801 gccgagcggg cggccgggga cctgcgcacc gtgctggacc accagaacgc ctgggtgtat
     4861 gtggtggagc ggggcagctg gaagctgcgc tatgccaacg gcaagacccg gcgcctggcg
     4921 ccgcaggccc gcgagggcgc ggtctgttat cgcgcccttt tcggccgcag cgagccctgt
     4981 gcccactgcc ccgtgcgcga gggcgcgacg gccggcggca ctgagatgta taacggccgc
     5041 ctgggggtct gggcgctggt gaacgccacc gccgtgcagt gggaggggac cccctgccat
     5101 ctgctcacct gccaggatat caccgactat aaaaagtagc cgctttgcgg ctactttttc
     5161 ggtcctgcgg cccatccccg gtcctgcggc ccttgaggga agagcaggag cttcccccgc
     5221 tgcgaaaatc cccagggcgc acacgctttg ccgctttccg gcaaggggaa gcggcgcggc
     5281 gcagccgcgc cggaggggcg gggagagagg aaccggcagg cgggaagccc gccccgcgcg
     5341 ggtgaaagcg aaaccgcgtc ggggagcgag gctgcgctga aagggtgggc gtgaaaccgc
     5401 cgcagggccg ccccgaaagg cccggcccgc ggcggcgaag ctgacgggac gctcttcggg
     5461 gctttcgcct ttctttcgcg ccgccctccg cccccagggc cgctttgcgg cggtttcctc
     5521 tctctgtcaa gcggtgcgcg ggccgcctct gggccctgcc cgtgagaaaa agccggactt
     5581 tcgcccggta catccggaat attttaacaa aaaaagacaa agaggccttt atggacgaag
     5641 agaaaaaaca gagggtggaa gccttcgcgc acgagatcct gtacagctat tacaccgagt
     5701 cggatgtgga gctgctgatc gcacatctgg cgccggaggt ggtctggctg ggcgcggggc
     5761 gaaaccagcg cgccgagggg cgcgaggccg tggccgcggt ctttcgcgcc ggcagggaag
     5821 agctgttccg ctgcgagata agcggggagc ggtacgtcac ccgcagcctc ggcggcggct
     5881 gctggctgtg ccagggcgac agccaggtga cctccgcgcc gggcaccggc aagtacatcc
     5941 aggcccacca gcgcatcacc tttctttttc aagagcaggg gcagcggctg ctgatcgccc
     6001 acatccacca ctcgatggac tataccggcg tggcggacgg cgagctgttc cccgtgaaag
     6061 ccgggcaggc ggcctatcaa aagctgcagg ccaccctgga ggaaaaggac cgtcagattg
     6121 agctgatgct cacgcagatg cccggcggca tggccatctg cctgccggac gaggatttca
     6181 ccgtggactg gatcagcagc agcctgtgca agctgctggg ctatgcgggg cccgaggagt
     6241 tttttgccgc caccggcggc gcctgccgcg cgttcatcca gcccgaggac tacgatgaga
     6301 tggcgcggaa agtggagggg cgcttttccg actccgacga gtactatgcc gaataccggg
     6361 tgcgcctgca agacggcggc gccctctggg cgtccgacct ggggcgcagg ataacggatg
     6421 cggacggccg caccctgatc tactgtttta tctccgacat tacccgccgg cgcgagcggg
     6481 agctggagat cgagcgggcg aaccgggagg cggcccggca ggcgcgcttc ctcgggcagc
     6541 tgtacgagtc catcccctgc ggcattttgc agttcccccc cgggcccgag caccgcatca
     6601 tcagcctcaa ccccatggtg tggcggtttt acggcttcgg ttcagaggcc gagtaccgcg
     6661 ccgcggtctc cgacccgttt gacctggtgc tggaggagga gcggccccgg ctgcagcggc
     6721 tggtggacgg cctgccgctg gggggcggca ccatctcgta tacgcggcag gtgcgccgca
     6781 gggacgggcg cgagggctgg atcagcgtgg tgctggagcg gctggtcaac atcgacggcg
     6841 ttgaggtgct gcaggcgatc ttcaccgata tcaccgagat caaagcgctg caggccgcgc
     6901 aggagcggga gcagctgctc gagaaccggg tgctgcgcgc ggccatctgc accgcctacc
     6961 ccatgatcat gagcctcaac ctgacccgcg gcacctacag ctgctttgtg gatcagcagg
     7021 gcttttacga cgggcggcgg cagggcgatt ttgaggagat ggcgcgggag gcggtagcca
     7081 acacctatcc ctcctaccgg gaggacttcg cccgctttct ctcgcgggag gagattttgc
     7141 gccgcttcgc cgcgggcgag caggagcttt ataccgagct gcgccagctg ggggtggacg
     7201 gcgcctatca ctggaactcg gtgcagctca tccaggtgga aaacccggtg ggcgacgagc
     7261 tgatggccat tgagctggtg cgggtgctgg acgagcagcg ggccgaaaaa gcgcggcagg
     7321 agcagctttt acgggatgcg ctggcggcgg cgcgggcggc gaacagcgcc aagtcggatt
     7381 ttctctcccg catgagccat gatatccgca ccccgatgaa cgccatcatc ggcatgagca
     7441 ccatcggcca gctcaaggcg ggggaccccc aggcggtgcg caactgcttt gccaagatcg
     7501 acacctcctc gcgctttctg ctctcgctga tcaacgacat cctggatatg tccaagatcg
     7561 aaaacgggaa aatggccatc gcggaggagc gctttgattt caccgccttt tttgacgagc
     7621 tggtctcgat catctacccc caggccgaaa aggccggcct ggaatttgag gtgcacatct
     7681 ccgcggcgct ggagcgctgg tatgtggggg atgagctgcg gttgaagcag gttttgatga
     7741 acctgctctc caacgccctc aaattcaccc cgcgcggcgg caggatcgtg gtggaggcgg
     7801 cggaggagcg ccgcgccgca ggcaaggccg tgctgcgctt tgacgtgagg gacaccggca
     7861 tcggcatgtc ggaggagttt ctcacccgca tcttcaaccc ctttgagcag gaggaggaga
     7921 gcgcggcgcg caacaatgtg ggcagcggcc tggggctgtc gatcgtctac aacctcgtcc
     7981 agctcatggg cggcgaggtg caggtgcaca gccgccgcgg ccagggcgcg gcctttgccg
     8041 tgcggctgcc cctgggcctg ctggaggacg acgcggagga ggagcagcgc cgcagggccc
     8101 gcgagctgat gcagggcgcg ccggtgctgg tggcggacga cgaccccgcc gtgggcgagc
     8161 aggcctgcct gatcctgggc ggcatcggcg cgcggccgga gtgggccgcc tccggggccg
     8221 aggcggtgga aaaggtccgc tgtgccctgg cccggggcga gcactacgag atcgccctca
     8281 tcgactggcg catgcccggc atggacggcg tcgagaccac ccgtcgcatc cggcagctgg
     8341 tggggcccga gaccaccatc gtgatcatct cggcctacga ctggagcgat atcgagcagg
     8401 aggcgcgcgc ggcgggggcg gatcattttc tctcaaagcc gctgttcagc tccacgctct
     8461 gtcacgccgt cacccatctg gaaaacgcgg ccgcccgcgg ccattccacc gtggacaggg
     8521 cgctggccgg gcggcgggtg ctgctggtgg aggacaacga gctcaatcgg gagatcgccc
     8581 gctcgctgct ggagctctac ggtttggaag tggataccgc ggagaacggg cgcgaggctg
     8641 tagagcggtt tgcagcgcgg gaggccggca cctacctggc ggtgctgatg gatatccgta
     8701 tgccggtgat ggacggcctc gaggccaccc gcgccatccg cgccctgccg cggggggatg
     8761 cggcgagagt gcccatcctg gcgatgacgg ccaacgcctt tgacgaggac cgcacccagg
     8821 cctaccgggc gggcatgacc ggatatctga ccaagccctt ggatatccag gtgctgctgg
     8881 aggctttgga aaagctgctg cccgcgccgc ctgaaaagcc ttgagaggcg gccggtgaga
     8941 cggcttttgt gaaataagga gagacggacg gcccggccct gccagacgcg ggggccgggc
     9001 cgttttcttt tgggagcctg aggcggaaag gcgcgcggat gctccgtgct tttccgccgg
     9061 cttttgtgcg ctgcaaaagc ggaaagtgtg tctgcccacc acacctggca gggggaggag
     9121 gctcccgcaa agcgggggcc ggaggggctg cggcgctgcg gccctaaaaa gataaaagtg
     9181 ctgagcccac agcacctccc ctcaggcggc ccttggcccc gggcgccccc gcagggggag
     9241 ccggagggag gctcggcgcg gcggcgtggg aaaaaccgcc ggtggcgcgg gctgcaaaaa
     9301 cgatgaaaaa gaggcggcgc tgcgccgccg cccgcaccgc aaaaaatgct tgacaaccga
     9361 aagctgctct gttatactgg ccaattagtt aagcgattat aattaatttt tctgaactat
     9421 aggaggcggc catggatctg cggcagagga tggagcggct ttttgtggac cggaccgtga
     9481 gcgagctgca gctcgccaac agcgacacct atcaaagcgc gtgacctaca acagcctgct
     9541 ctatctcgat ctcatcgctt atacgccgga ttgcaccgcg ggctatctgg ccgagacgct
     9601 gcacgtcgcc ccctcggcga tcacctccaa ggtgcgcgag ctggagcgcc tgggcctggt
     9661 cacccgcacc cgcagcgagc gggacaaacg ggtctattac ctgcacatca acgaacaact
     9721 gcgggccgag taccgcgcct acgaccagat tctctacggc gcgctggacg agctggaggc
     9781 gcgctacacg ccgcggcagg tggacctgct gtgcgagatg ctggacgcga tcaaccgcgg
     9841 ctttcagcag gcaaaaacag cccgggcgct gccgggtgga aaagagaaga aaggaaaaga
     9901 aacgtgaatt cccttgaaaa aaagatcacc ccgggctttt tgctgcgctt caccctgccc
     9961 acggtggtca tgatggtctt caactccttt tacaccatgg tggacggggg ctttgtctcc
    10021 cgtttcgtcg gcaccggggc gctgtcggcg gtgaacatcg tctaccctgc cctcaacctg
    10081 gtctttgcgc tgggcatcat gcttgccacc ggcggcagcg ccgtggtggc caagctgctg
    10141 ggggagggca gagaggagca cgcccgccgc agctttacca cgctggtggt ggtggccctg
    10201 gccctggggg cggcgatgac cgctgccggc acgctgctgg ccgggcccgg cgtgcgcatg
    10261 ctgggcgcca acgacgcggt cttcgcctac tgccgcgact acatgttcac gctcagcctg
    10321 ttcgtgcctt tcgccatgct gcagctgctg ttccagtatt tcttcgtcgc cgcggcgcgg
    10381 ccgaacctgg gcctgatctc caccgtggcg ggcggcgtgg ccaatatcgt cctggattat
    10441 gtctttatcg tgcccctgaa gatgggggtg accggcgcgg cgctggccac cggcctcggc
    10501 tttgtgatac cggcggtggt ggggcttgtc tttttcgcct gcaaccgcaa gggggcgctg
    10561 catttcgccc gcccccggtg ggagccgcgg ctgctcgcct gtgcctgcgc caacggttcg
    10621 tccgagatgg tctccaacct gtcggtggcc gtgaccacct ttttgttcaa tctcctgatg
    10681 atgcgggccc tgggcgagga cggcgtggcc gccatcacta tcgtgctcta ctcccagttc
    10741 ctgttcacgg ccgtgttttt gggctatgtc tcgggcgtgg cgcccctctt cagcttcaac
    10801 tacggcgcgc gggacaaaaa gcggctggga cagctgttcc ggctcagcct catctttatc
    10861 gccgtctgct cgctgctggc ctgggcgctc tcgctggcgc tgtcgcagca ggtggtaagc
    10921 gttttcgcaa agcgcggcga gccggtcttt gacctggcgg tccacgggct gttcctcttt
    10981 tcggccggct ttctgtttat gggcttcaac atctttgcct cggggctgtt caccgccctg
    11041 tccaacgggc gggtgtccgc catcctctcc tttctgcgca cctttgtgtt catcgtggcg
    11101 gccatcctgc tgctgccccg gctgctgggg gtggacggca tctggctctc gatccccgcg
    11161 gcggaggcgc tggccctgct ggtctcggcc tggtacatgg tgcggctgaa gccggtgtac
    11221 ggctattgag gtgcgggggc gcgcagggtg ggcggcccgc ttcggcgcgg tcccgtcccg
    11281 gccgggcggc cgccccgctc tgacgggacg ggcgccgccg cgcggctttg cgcggtaggg
    11341 taaagcccga cgttcccctg ccgccgccgc gctttgcgga gccgcccttt tcgccctctt
    11401 ttcagcggat acaaaacgct tttgcatgcc gccgccccgt gctataatag gggcgaaacc
    11461 ggcgggcgcc cgcccgcgat gaggagg
//
LOCUS       DBMDYA010000008        11190 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1651_length_11190_cov_1.434845, whole genome shotgun sequence.
ACCESSION   DBMDYA010000008 DBMDYA010000000
VERSION     DBMDYA010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1651_length_11190_cov_1.434845"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="ACZPFK_00475"
     CDS             <1..1534
                     /locus_tag="ACZPFK_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017745.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR1404813.1"
                     /translation="TEALLKELKAFDAEKLDDASRLTLEVLQDYLQRSLLLGEQFYEQ
                     DNSYLGSFIGFQAQLPLLLAEYKIRTQRDLDSFYHLMETSGETFEKYAEMERQRQESQ
                     VGMGPTIMKAVIGQCENFAAGDVGFLVDAIAEEIDTADFLSDEEKSAAREKSEALIRG
                     DFVDAYRSLGESLAAIEINSTDLGLAHQPGGKEYYETLLQVRTGVDMTVEEVRDYFES
                     KLEEDIQAFTDQLSLLYRQDPQRTEEFLDTLEVDYGNFTSAEELVDYLRRQCAADYPA
                     LDRLNYTVTPVAEAMQENFSPAAYLQGRIDAPQSEAEVIYINGAFTSDLFTTVAHEGY
                     PGHMYQHSYFKSLGLPTVRYMIDYNGYSEGWATYIEHNSVKYLPTGDAGYAAALESNS
                     KIAEDVIALSDIGIHYDGWDREQYAAFMNNYFSMDEELLDGQFDLNLETPTNYLQYSL
                     NGRLFQDMHDEAQEALGEQFSETEFHRVLLDTGPAPVSLIQAQVDAWVDGLLPDARPK
                     AA"
     gene            complement(1677..3065)
                     /locus_tag="ACZPFK_00480"
     CDS             complement(1677..3065)
                     /locus_tag="ACZPFK_00480"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR1404814.1"
                     /translation="MEFSQLKAAAEQFAAQHEQEIVADIARLVAIKSVRGAPQPGAPF
                     GPGPRDALQEALSLAAARGLAAENLEVVGRASTPGSGGKTVGIVAHLDVVPEGGGWNT
                     DPYTLTEREGWLLGRGVADDKGPAVLAIWAAKFFADLPQRPRHTIQVLLGTSEETGMS
                     DLKQYLEAHPAPDFSFTPDGSFPICNGEKGGFDGEFVSGPLEGVLAAFEGGLAANSVP
                     DRAVAVVRLPADSLPAAEDISVTADAQGARIEARGIAGHASKPEGSRSAIGMVAAYLL
                     ENRLVDGKERAALELVRDLAVSHDGELLGIAARDEVFTPLTIIGGQIRMENGRLIQNF
                     DCRYPTSITGEELGQRLGRRAQAAGMTLQNAVANPPFYIEPDSPPILALRRTYREITG
                     RDDAPFTMGGGTYARRLPRAVSFGIGESGMERAPFVGSIHGANEGFPLKKLMESLVIF
                     ICALGRLMEIDL"
     gene            3386..3730
                     /locus_tag="ACZPFK_00485"
     CDS             3386..3730
                     /locus_tag="ACZPFK_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1404815.1"
                     /translation="MKKGKFFKKRDLIVLLVLLALAAGIGLFYLTRGAGAKATVTVDG
                     GGSWEIDLSKDNIYHIENAALPVTLEVKDGKIRFIDAQCPDHLCEGFGFIGNEGEYAI
                     CMPAGVAVNIYG"
     gene            3956..5734
                     /locus_tag="ACZPFK_00490"
     CDS             3956..5734
                     /locus_tag="ACZPFK_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1404816.1"
                     /translation="MDIFSVISLAGGIALFLYGMSIMGSGLEKLAGGKMEVILKKLTS
                     TTIMAVISGAVLTGLIQSSAATTVIVIGLVNSGIMQLPQAIGVIMGANIGTTVTGQII
                     RLADLGGDSIFLQLLKPTTIAPVFAFAGAILFVFLKTPSKRNIGQILIGFGILFTGMF
                     TMESSVAPLKDSPLFLKLFASLENPILGIIAGALVTIAIQSSSASVGILQALSTTGVV
                     TWGSAIPIILGQNIGTCSTPLIASIGASKGAKRAAFVHLYFNLIGTFVFMVVLYGIKY
                     TVGLPFWEGVMNRGDIANFHTFFNVVTTLFFIPFTKLLAKLATLTIPDKGDETVDTDM
                     PVLDERLFGSPSVAIQQARGAVEKMARNARLNYEVAIPLLYSHDLEAIAQVNAREDVV
                     DKLEYYIGNYLVKIADRELSETESRSVSDLLAYITEFERIGDYSINVVERSGEIFDKE
                     ISFSESARRELNVLDGAIKEIIRLAERSFRHNDAALAASVEPLEETIDSICDLLHNRH
                     IERLQNGQCAIQSGVVYLEVLTNLERIADHCSNIAARVIGNEADDGQFDAHEMRRKLH
                     DGYVEDYNDRLQSYELKYLSPIREEG"
     gene            6062..6622
                     /locus_tag="ACZPFK_00495"
     CDS             6062..6622
                     /locus_tag="ACZPFK_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1404817.1"
                     /translation="MLEDCAIVELYWQRSEEAIRETARKYGAYCRTIIRNILGSSEDT
                     EECENDTWLSAWNAMPTKRPDRLAPFLGRIARNHALDRYDYNTAKKRAAPAALLIDEL
                     ADCLPGGQEVEEALDERELAALVTAFLRTQSETARGVFLRRYWYAEPIEAIAARYGLT
                     QVNTRTLLSRTRARLRAYLEKEGVSA"
     gene            6619..>6928
                     /locus_tag="ACZPFK_00500"
     CDS             6619..>6928
                     /locus_tag="ACZPFK_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404818.1"
                     /translation="MRGEKLLRAIGLVEDDLILEADAPPAPKKKKTVPWRWLSLAACA
                     VLACMATLLLRTGGYSSAAPEAGAEALDENALQGQRPGPGAGGAEAGPAPFALGESGS
                     G"
     assembly_gap    6929..6938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6939..7928
                     /locus_tag="ACZPFK_00505"
     CDS             <6939..7928
                     /locus_tag="ACZPFK_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404819.1"
                     /translation="FEGLLEFAPSELEQGCPWRPGDQVDSLPVYKNGSSLYLSGVAQF
                     TLDAPQMEARLRELAAKLGYTVEEVEVYPSENQVEEMKQKLGDADDLTIELNTRPSRV
                     TAECDGAVLRMDTGGDLTVEFSEPVSLPAGYRFSADATAEEAQQAVRYLWSIYGEPLG
                     LAQPAPRLFLDYTYEGKPHREFAVFDGSGDLADRLLQFSLGGLTFWDDEQGRLYGFRL
                     SGTDVVGEKLGDYPVLSYEEAKELLAQGNCFTSVMEEFPGVERLAYTELVYRVSSGVV
                     APYYRFLVELPTLEMENGLKTYGAYYVPAVESAYITDLPAAQWGYTAPKMPQQ"
     gene            8047..8568
                     /gene="bilS"
                     /locus_tag="ACZPFK_00510"
     CDS             8047..8568
                     /gene="bilS"
                     /locus_tag="ACZPFK_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR1404820.1"
                     /translation="MNYAVVYISKTGNTAKVARAIRDALPRGGCLAYGAPGEAATRAD
                     LIFAGSWTDKGACNEEMAAFLAGLSGKKVALFGTAGFGGSREYFDTVLARMAKVLPAD
                     CEMAGGFLCQGRMPQAVKDRYEAMLKAQPLDAQARAMLDNWEAAYPHPDEEDLAAARA
                     FALRIVRESSGEK"
     assembly_gap    9059..9068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9069..9453
                     /locus_tag="ACZPFK_00515"
     CDS             <9069..9453
                     /locus_tag="ACZPFK_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013231.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1404821.1"
                     /translation="GCTQAEVAQNMMVTPASMALTTKRLEKAGLLQREVDESNQRCKR
                     LSVTARGHDLAARCRDFHDEFDQRTFDGFSEEELAALNVFLGRMICNITGEDYPPQDG
                     FVPIGQLEARLRDSRREREEREERK"
     gene            9456..11180
                     /locus_tag="ACZPFK_00520"
     CDS             9456..11180
                     /locus_tag="ACZPFK_00520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404822.1"
                     /translation="MKQLLSCVGKYKKQTLLTPFMVVGEVVVDIMIPLLMSRIIDVGI
                     QNRDVGYVARVGGLMVLCALVALAFGALSGRFAAVASMGFAKNVRRKLFYKIQDFSFA
                     NMDKFSTASLVTRLTTDVTNTQMAYMMCIRMLVRAPIMLICATAMTVSINARLALVFF
                     VAIPVLGTVLYLIATRAYPRFQAMLTRYDGLNARVEEGLIAIRVVKAFVRHEYEEEKF
                     EKEADALRAAQKRAEKLVILNMPAMQLTMYACILSILWFGGNMIVGGTMGTGELMSFI
                     SYVTQILMSLMMLSMVFVNLVLSRASVRRITEVLDEVPDIDDSAADPGAQMEDGSIEF
                     KDVSFSYSHKADNLTLQHIDLSVKSGETIGIIGGTGSAKTTLVQLIPRLYDIDGGELL
                     VGGRDIKEYPLETLRDGVAMVLQKNVLFSGTIDDNLRWGDEHASEDQIREACRAADAD
                     GFVSAFPDGYETDLGQGGVNVSGGQKQRLCIARALLKRPKIVILDDSTSAVDTATDSR
                     IRGALRTQLAGTTTIIIAQRITSVMDADRIVVLDDGKISAVGTHDELMQSSEIYREVY
                     ESQQKGVA"
BASE COUNT         2166 a         3317 c         3645 g         2042 t
ORIGIN      
        1 gacggaggcg cttttgaaag agctgaaagc gttcgatgcc gaaaagctgg acgatgcttc
       61 ccgcctgacg ctggaggtgc tgcaggatta cctgcagcgc tcgctgctgc tgggcgagca
      121 attttatgag caggacaaca gctatctggg cagctttatc ggttttcagg cgcagctgcc
      181 cctgctgttg gcggagtaca agatccgcac ccagcgcgat ctggacagct tttatcatct
      241 gatggagacc agcggggaga cctttgaaaa atatgccgaa atggagcgcc agcggcagga
      301 gagccaggtc ggcatgggcc ccacgatcat gaaagcggtg atcggccagt gcgaaaactt
      361 tgccgcgggg gatgtgggct ttttggtgga cgccatcgct gaagagatcg acacggcgga
      421 tttcctctct gacgaggaga agtcggcggc ccgggagaag agcgaggccc tcatccgcgg
      481 ggattttgtg gacgcctacc gcagcctggg cgaatcgctg gccgccatcg agatcaacag
      541 caccgatctc ggcctggccc accagcccgg cgggaaagag tactatgaga ccctgctgca
      601 ggtgcgcacc ggcgtggaca tgacggtgga agaggtgcgg gactattttg aaagtaagct
      661 ggaggaggac atccaggcgt ttaccgatca gctcagcctg ctctaccggc aggaccccca
      721 gcgcaccgag gagtttttgg acacgctgga ggtggattac ggcaacttta cctccgcaga
      781 ggagctggtg gactatctgc gccggcagtg cgccgccgat tatccggccc tggatcgctt
      841 gaactacacc gtgacgccgg tggcggaggc catgcaggaa aacttttctc ccgccgccta
      901 tcttcagggc cgcatcgacg cgccccagag cgaggcggag gtcatctaca tcaacggcgc
      961 gttcacgtcc gacctgttca ccaccgtggc tcatgagggg taccccggcc atatgtacca
     1021 gcacagctat ttcaaatcgc tgggcctgcc caccgtgcgc tatatgatcg attacaacgg
     1081 ctacagcgag ggctgggcca cctatatcga gcacaacagc gtcaagtatc tgcccaccgg
     1141 cgacgcgggc tatgccgccg cgctggagag caacagcaag atcgcagagg acgtcatcgc
     1201 cctttcggat atcggcatcc attacgacgg ctgggaccgg gagcaatacg cggcgttcat
     1261 gaacaactat ttttccatgg acgaggagct tcttgacggg cagtttgacc tcaatttgga
     1321 gacccccacc aattatctgc agtattcttt aaacggcaga ttgttccagg atatgcacga
     1381 cgaggcgcag gaggcgctgg gggagcagtt cagcgagacg gagttccatc gcgtgctgct
     1441 ggacaccggc ccggccccgg tctcgctgat ccaggcgcag gtggacgcct gggtggacgg
     1501 cctgctgccg gacgcgcgcc cgaaagcggc ctaggccgga agacgaaaaa gggccgcgcg
     1561 ccgatttctc gcaggagaaa gggcgcgcgg tcctttttaa aacagggcaa aagcccgctt
     1621 gggcaaaaac ggcgggcaat tcaggcgccg ccggtcaaaa gactagggcg gcggccctac
     1681 agatcgatct ccatgagcct gcccagcgca cagatgaaga tcaccaggct ctccatcagc
     1741 ttcttcagcg gaaaaccctc gttggcgccg tggatggaac cgacaaaggg cgcgcgctcc
     1801 atgccgcttt ctccgatgcc gaagctgacg gcccggggca ggcggcgggc gtaggtgccc
     1861 ccgcccatgg tgaacggcgc gtcgtctcgg ccggtgatct cgcggtaggt ccggcgcagc
     1921 gccaggatgg ggggcgagtc gggctcgatg taaaacgggg gattggcgac cgcgttctgc
     1981 agcgtcatgc ccgcagcctg ggcacggcgt cccaggcgct gccccagctc ctcccccgtg
     2041 atggaggtgg gatagcggca gtcgaagttt tggatcagcc taccgttttc catgcggatc
     2101 tggccgccga tgatggtcag cggcgtgaac acctcgtccc gggcggcaat gcccagcagc
     2161 tcgccgtcat gggagacggc gagatcgcgc accagctcca gcgcggcgcg ctctttgccg
     2221 tcgaccaggc ggttttccag cagataggcg gcgaccatgc cgatggcgga gcgggagccc
     2281 tcgggcttcg acgcgtggcc cgcgatgccg cgggcctcga tgcgggcccc ctgtgcgtcg
     2341 gcggtcaccg agatgtcctc cgcggcgggc aggctgtcgg cgggcaggcg caccaccgcg
     2401 acggcgcggt cgggcaccga gttggccgcc agaccgccct cgaacgcggc cagcacgccc
     2461 tccagcggcc cgctgacgaa ttcgccgtca aagccgccct tttccccgtt gcagatgggg
     2521 aaagagccgt ccggcgtgaa cgagaagtcg ggcgcggggt gggcttcgag atactgcttc
     2581 aggtccgaca tgccggtctc ctcactggtg cccagcagca cctggatggt gtgccggggc
     2641 cgctgaggca ggtcggcaaa aaacttggcg gcccagatgg ccagcacggc gggtcccttg
     2701 tcgtccgcca cgccgcggcc cagcagccag ccctcccgct cggtcagggt gtaggggtcg
     2761 gtgttccagc cgccgccctc gggcaccacg tccagatggg cgacgatgcc caccgtcttg
     2821 ccgccggacc ccggcgtcga cgcgcggccc accacctcga gattttcggc ggcgaggccg
     2881 cgggccgcgg caagcgagag ggcctcttgc agggcgtccc gcgggcctgg accaaagggc
     2941 gcgcccggct gcggcgcgcc ccgcacgctt ttgatcgcga ccagacgggc gatgtcggcc
     3001 acaatctcct gttcatgctg cgctgcgaat tgttccgcgg cggctttgag ctgtgaaaac
     3061 tccatatctt acctcctggc gggcggctgc ccgcattgtg atgctgtgtt tcccctgtgc
     3121 gccggggcat gggcatatcg gcggccacgg cgcgttccgt gaaaagcgcc ttgtgtgccg
     3181 cctgaaaaaa gcggccttcg cctctctgat cattatatca tttcggcggc gtctttaaaa
     3241 ccgggcgcgg cagcggctct cccgcgcttg cagccgctct ggggccggct cgctctttct
     3301 gaaagtgaaa acagcgcgga tgaggcggat atggtatact gataccatac cgcgaaagac
     3361 atttaaaaaa tatcgggaga cctgcatgaa aaaaggaaag ttttttaaaa aacgcgatct
     3421 catcgtgctg ctcgtcttgc tggcgctggc ggcggggatc gggctgtttt atctcacccg
     3481 cggcgcgggc gcaaaggcca ccgtcaccgt ggacggcggc ggttcttggg agatcgatct
     3541 gtccaaagac aatatctatc acatcgagaa cgccgccctg ccggtcacgc tggaggtaaa
     3601 ggacggaaag atcaggttca ttgacgccca atgcccggat catctctgcg aggggttcgg
     3661 ttttatcggc aacgaggggg aatacgcgat ttgtatgccc gccggggtag cggtgaacat
     3721 ttacggatga cgctttttta aagagagaga cgattttggg caaactaccc gaaagcatct
     3781 ctcttttttg tgcatcatta cgttttttaa tgagaccatt acaaaaacta taccattccc
     3841 ttaccaaatg atgctatact gattttgctt gcggattttg tcctgctttg acgaagcacg
     3901 ccaaaaccgc aggggacaag aagaacagca aaaacagacg acagcggggg atacgatgga
     3961 tattttttcc gttatttcac tggccggcgg cattgccctt tttctttacg gcatgagcat
     4021 catgggctcc ggcctggaaa agctcgcggg cggcaagatg gaggtcatcc ttaaaaaact
     4081 cacctccacc accatcatgg cggtgatctc cggtgcggtg ctcaccggcc tgatccagtc
     4141 ctcggcggcg accaccgtca tcgtcatcgg cctggtcaac tccggcatca tgcagctgcc
     4201 ccaggccatc ggggtgatca tgggcgccaa tatcggcacc accgtcaccg gccagatcat
     4261 ccgtctggcg gacctgggcg gcgacagcat ttttctgcag ctgctcaagc ccaccaccat
     4321 cgcgccggtc ttcgcctttg cgggggccat cctgtttgtc tttttgaaaa cgccctccaa
     4381 gcgcaatatc gggcagatcc tcatcggctt cggcatcctg ttcaccggca tgttcaccat
     4441 ggaatcctcg gtggcgccgc tgaaagacag cccgctcttt ctcaagctgt tcgccagcct
     4501 ggagaacccg atcctgggca ttatcgcggg tgcgctggtc accatcgcta tccagagctc
     4561 ttcggcctca gtgggtatcc tgcaggcgct ctccaccacc ggtgtggtca cctggggcag
     4621 cgccatcccc atcattctgg gccagaacat cggtacctgc tccaccccgc tcatcgcctc
     4681 catcggcgca tccaaggggg ccaagcgggc ggcttttgta catctctact tcaacctcat
     4741 cggcaccttt gtcttcatgg tggtgctcta cggcataaaa tacacggtgg gcctgccttt
     4801 ctgggagggt gtgatgaacc gcggcgacat cgccaacttc cacactttct tcaacgtggt
     4861 caccaccctg ttcttcatcc cctttaccaa gcttttggca aagctggcca ccctcaccat
     4921 cccggacaag ggcgacgaga cggtggacac cgacatgccg gtgctggacg agcgcctgtt
     4981 cggctcgccc tcggtcgcca tccagcaggc gcgcggcgcg gtggaaaaga tggcccgcaa
     5041 cgcccgtctc aactacgagg tggccatccc tctgctgtac agccatgatc ttgaggccat
     5101 cgcccaggtg aatgcccgcg aggacgtggt ggacaagctg gagtattaca tcggcaatta
     5161 tctggtcaag attgccgacc gcgagctgtc cgagaccgag agccgcagcg tctccgatct
     5221 gctcgcctat atcaccgagt tcgagcgcat cggcgactac agcatcaacg tggtggagcg
     5281 cagcggcgag atctttgaca aggagatcag tttttcagag tccgcccggc gtgagctgaa
     5341 cgtgctggac ggcgccatca aggagatcat ccgtctggcg gagcgcagct tccgccacaa
     5401 cgacgcggcg ctggccgcca gcgtggagcc gttggaggag acgatcgaca gcatctgcga
     5461 cctgctgcac aaccgccata tcgagcggct gcaaaacggg cagtgcgcca tccagagcgg
     5521 cgtggtctat ctggaggtgc tcaccaatct ggagcgcatc gcagaccact gctccaacat
     5581 tgcggcgcgg gtcatcggca acgaggcgga cgacggccag ttcgacgcgc acgagatgcg
     5641 ccgcaagctg catgacggct atgtggagga ttacaacgac cgtctgcaga gctatgagct
     5701 gaaatatctc tctcccatcc gggaggaggg ctgaaaaaat agccgctatg cggctatttt
     5761 ttttggctgt gcggcccggg gacgggccac ggtcgcagag ataagctttt tgttgcgaag
     5821 caataaaaat agccgctgtg cggctatttt ttacgcgcgc ccgcccacgg aagtgggcgg
     5881 gcgcttaaga tttcaaggcc gtggccagag gactggccac ggccatagaa atgagttgtt
     5941 tttattgcga agcaataaaa aacatctccc gcttgccaca tcccgtccgc ttttcctatt
     6001 ataataacag aagaggtcga aaaaggtgta gcttgtgtta ttggggaaag gagggaccca
     6061 catgctggag gactgcgcca tcgtcgagct gtactggcag cgcagcgagg aggcgatccg
     6121 cgagacggcg cgcaaatacg gggcgtattg ccgcaccatc atccgcaata tcctgggcag
     6181 cagcgaggat accgaggagt gcgaaaacga tacctggctc tccgcctgga acgccatgcc
     6241 caccaagcgg ccggaccggc tggcgccctt tttgggccgc attgcccgca accacgcgct
     6301 ggaccggtat gactacaaca ccgcgaaaaa gcgggccgcc cccgccgcgc tgctcatcga
     6361 cgagctggcg gactgcctgc ccggcgggca ggaggtggag gaggcgctgg acgagcggga
     6421 gctggcagcc ctggtgacgg catttttgcg cacccagagc gagacggcgc gcggcgtgtt
     6481 tttgcgccgc tattggtatg cggagccgat cgaggccatc gcggcgcggt atgggctgac
     6541 acaggtcaac acccgcaccc tgctcagccg cacccgcgcg cgtctgcggg cttatctgga
     6601 aaaggagggg gtgtcggcat gagaggggaa aaactgctgc gggccatcgg cctggtggag
     6661 gacgacctga tcctggaggc ggatgcgcct ccggccccga aaaaaaagaa gacggtgccc
     6721 tggcgctggc tctccctggc cgcctgcgcg gtgctggcct gtatggcgac gctgctgctg
     6781 cggacaggcg gctattcctc cgccgcgccg gaggcgggcg ctgaggcgct ggatgagaac
     6841 gccctgcaag ggcagcgccc tggtcccggc gcaggtgggg cagaggccgg gccggcgccc
     6901 tttgccctgg gtgaaagcgg gagcggcgnn nnnnnnnntt tgagggcctt ttggagtttg
     6961 cgccctctga gctggagcag ggctgcccct ggcggccggg cgaccaggtg gacagcctgc
     7021 cggtctataa aaacggcagc agcctgtatc tcagcggcgt ggcccagttc actctggacg
     7081 ccccgcagat ggaggcccgc ctgcgcgagc tggccgcgaa gctgggctac acggtagaag
     7141 aggtagaggt ctatcccagc gagaaccaag tggaggagat gaagcagaag ctgggggacg
     7201 cggacgattt gacgatcgag ctgaacacgc gcccctcccg ggtgacggcg gagtgcgacg
     7261 gcgctgtgct gcggatggat acggggggag atctgacggt ggagttcagc gagcccgtgt
     7321 ccctgccggc ggggtatcgc tttagcgcgg acgccaccgc cgaggaggcg cagcaggcgg
     7381 tgcgctacct ctggtcgatc tacggcgagc cgctggggct cgcgcagccc gcgccgcgcc
     7441 tcttcctcga ctacacctac gagggcaagc ctcaccggga gttcgccgtc ttcgacggca
     7501 gcggtgacct ggcggaccgg ctgctgcagt tcagcctggg cggcctgacc ttttgggacg
     7561 acgagcaggg gaggctgtat ggcttccgcc tgtcggggac cgacgtggtg ggcgaaaagc
     7621 tgggcgatta cccggtactc tcttatgagg aggcgaaaga gctgttggca caggggaact
     7681 gctttaccag cgtgatggag gagttccctg gtgtggagcg tctggcctat accgagctgg
     7741 tgtatcgcgt cagctccggc gtggtggccc cctactaccg ctttttggtc gagcttccca
     7801 cgctggagat ggagaacggc ctcaagacct atggggccta ctatgtcccg gcggtggaga
     7861 gcgcctatat caccgatctg cccgccgccc agtgggggta taccgccccc aaaatgccgc
     7921 agcagtaaag ctgcgggcgg ctttcagaat aaatgagaaa aaggtgaaag ggcttgactt
     7981 tgggcccgcg cgggctatac tgtacctcac ggaacggatt ttttgaggac aggggacggt
     8041 gcagctatga attacgcggt ggtctatatc agcaaaacgg gcaatacggc caaggtggcg
     8101 cgggccatcc gcgacgcttt gccgcggggc ggctgtctcg cctacggcgc cccgggcgag
     8161 gccgcaacgc gcgcagacct gatcttcgcg ggctcctgga cggacaaggg ggcctgcaac
     8221 gaggagatgg cggcgtttct cgccgggctg tcgggcaaaa aagtggcgct gttcggcacg
     8281 gcgggtttcg gcggctcgcg ggagtatttt gacacggtgc tggcccgcat ggcaaaggtg
     8341 ctgccggcgg actgtgagat ggccgggggc tttttgtgcc aggggcgtat gccgcaggcg
     8401 gtaaaggacc ggtatgaggc catgttgaaa gcgcagccgc tggacgccca ggcgcgcgcg
     8461 atgctggaca actgggaggc ggcctatccg cacccggacg aagaagacct ggctgcggcg
     8521 cgggcgtttg cgctgcggat cgtgcgggag agcagcggag aaaaataaaa aacagcgggg
     8581 gccgtcccag gcggcccccg ctgtctgtca aaaagaagtg gaaaggggcg gactgccggg
     8641 ctttgagccg gtcgtcgaca gcggacagat ggggcgagat cagctgtctg cccgcttttt
     8701 tgacaggtcg cggccgggct cagagaacga ccacgacggt tggaaaggcg gacaggaaca
     8761 taaaacaaga gggatgcttt gcttttcatc aacttttcac acaaaaaaca gttgaccttt
     8821 ttggtcatcc tgcgtatact aattatagtt aagcgcttaa ctatttggcg cttaatattt
     8881 ttgcggagag ggacaaagag atggagaaac gacggggcga aatcgtgcgg gccgcccagc
     8941 ggctgcatga gatgtcgatg ctgcggcgca tcatcctgca aaagatgacc cagggcatcg
     9001 acctgcactt tgggcagctg cgggtggtgg agtatatcgc ccaccacgac ggctgcacnn
     9061 nnnnnnnncg gctgcaccca ggcggaggtg gcccagaaca tgatggtcac cccggcctcg
     9121 atggcgctga cgaccaagcg gctggaaaag gcgggcctgc tgcagcgcga ggtggacgag
     9181 agcaaccagc gctgcaagcg gctctcggtc accgcgcggg ggcacgacct ggccgcgcgg
     9241 tgccgcgatt tccacgacga gttcgaccag cgcacctttg acggcttcag cgaggaggag
     9301 ctggcggcgc tgaacgtctt tctggggcgg atgatctgca acatcaccgg cgaggattat
     9361 ccgccgcagg acggttttgt gcccatcggc cagctggagg cccgcctgcg cgacagccgc
     9421 agggagcgag aagagagaga ggagagaaaa taaacgtgaa acaactgctt tcttgcgtgg
     9481 gaaagtacaa aaaacaaacg ctgctcacgc cgttcatggt ggtcggtgag gtggtggtgg
     9541 atatcatgat cccgctgctc atgtcccgca tcatcgacgt gggcatccaa aaccgggacg
     9601 tgggctatgt ggcgcgggtc ggggggctga tggtgctctg tgcgctggtt gctctggctt
     9661 tcggcgcgct gtccggccgc tttgcggcgg tggcgagcat gggctttgcc aaaaacgtgc
     9721 gccggaagct cttttacaag atccaggatt tttcgtttgc caacatggat aagttttcca
     9781 ccgcgtcgct ggtgacaagg ctcaccaccg acgtcaccaa cacccagatg gcctatatga
     9841 tgtgcatccg catgctggtg cgcgcgccga tcatgctcat ctgcgccacc gccatgacgg
     9901 tcagcatcaa cgcccggctg gcgctggtct tttttgtggc catcccggtg ctgggcacgg
     9961 tgctgtacct gatcgccacc cgcgcctatc cgcgctttca ggccatgctc acccgctatg
    10021 acggcctgaa cgcccgcgtg gaggaggggc tgatcgccat ccgggtggtc aaggcctttg
    10081 tgcgccacga gtacgaagag gaaaaatttg aaaaagaggc cgacgcactg cgcgccgcgc
    10141 agaagcgggc cgaaaagctg gtcatcctca acatgcccgc catgcagctg accatgtacg
    10201 cctgcatcct ctcgatcttg tggtttggcg gcaacatgat cgtgggcggc acgatgggca
    10261 ccggcgagct gatgagcttt atcagctatg tgacccagat tttgatgtcg ctgatgatgc
    10321 tctcgatggt gttcgtcaac ctggtgctgt cccgcgcctc ggtgcgccgc atcaccgagg
    10381 tgctggacga ggtgccggat atcgacgaca gcgccgccga cccgggcgcg cagatggaag
    10441 acggctcgat cgagttcaag gacgtctctt tcagctattc ccacaaggcg gataacctga
    10501 cgctgcagca catcgacctg tcggtcaaga gcggcgagac catcggcatc atcggcggca
    10561 ccggcagcgc caagaccacg ctggtgcagc tcatcccgcg cctgtacgac atcgacggcg
    10621 gcgagctgtt ggtcggcggc cgggatatca aggagtatcc gttggagacc ctgcgggacg
    10681 gggtggctat ggtgctgcag aagaacgtgc tgttttccgg caccattgac gacaacctgc
    10741 gctggggcga cgagcacgcc agcgaagacc agatcagaga ggcctgccgg gccgcggatg
    10801 ccgacggctt tgtgtcggcg ttccccgacg ggtatgaaac cgacctgggg cagggtggcg
    10861 tgaacgtgtc cggcgggcaa aagcagcgcc tttgcatcgc ccgggccctg ctcaaacggc
    10921 ccaagattgt cattctggac gactccacca gcgcggtgga caccgcaacc gacagccgca
    10981 ttcgcggtgc gctgcgcacc caactggcgg gtaccaccac catcatcatc gcccagcgga
    11041 tcacctcggt gatggacgcc gaccgcatcg tggtgctgga cgacggcaag atcagcgccg
    11101 tgggcaccca cgacgagctg atgcagagca gcgagatcta tcgcgaggtc tatgaatcgc
    11161 agcagaaagg ggtggcataa atggcgggtc
//
LOCUS       DBMDYA010000009        10761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1715_length_10761_cov_1.637430, whole genome shotgun sequence.
ACCESSION   DBMDYA010000009 DBMDYA010000000
VERSION     DBMDYA010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_10761_cov_1.637430"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="ACZPFK_00525"
     CDS             <1..1024
                     /locus_tag="ACZPFK_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1404823.1"
                     /translation="MSPAYLERLFDPFSREEDTRTTVTEGTGLGMAIPKRLVDLLGGE
                     IRVQSREGEGSTFRVSLPLEIDEAPSREASFPGLRALVVDDGEVTRAYVCQTLRRLGA
                     EADCTATGAEAVKKVVSAQRAGQGYDAVILDWKMPGMDGLQTARQLRREAPGGLPILI
                     MSAYNWEEIAEQARAAGVTGFLQKPIFCSTLCEGLRRFVLGRTAPKKAAPGYDFTGRR
                     LLLVEDNELNREIAVELLQQVGAQVDTAANGELGVEKFRSAPEHFYALILMDVQMPVM
                     DGLSAARVIRALPRGDAAQVPIVAMTADAFAEDVAAAKQAGMSAHIAKPIELQQLYRT
                     IQELLP"
     gene            1376..2131
                     /locus_tag="ACZPFK_00530"
     CDS             1376..2131
                     /locus_tag="ACZPFK_00530"
                     /inference="COORDINATES: protein motif:HMM:NF039499.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HIR1404824.1"
                     /translation="MENEIEEAITQQQIRTGNDYTNIILHTAGKSIVSMREIIYLSTF
                     GYPDGALSLARNLYEHVIILAFFENHKYDTDFDDYVDDYYIDYDIQRNKALQYESENC
                     TKDSESLTVLKDEFDRLKQTAHHRMTGNYWWSGSSSFNQLVKAAIASIPEEHGKRFLH
                     GLHFVYKRACVSIHSSCIGNTLRLGADPDFVGIDTSPAIKGHSLPLWFATTSFVYIMG
                     VTYALLNLDYKTHDRTLNNLTLFLHQKESQASD"
     gene            complement(2493..2568)
                     /locus_tag="ACZPFK_00535"
     tRNA            complement(2493..2568)
                     /locus_tag="ACZPFK_00535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2533..2535),aa:Lys,seq:ttt)
     gene            complement(2773..4677)
                     /gene="thrS"
                     /locus_tag="ACZPFK_00540"
     CDS             complement(2773..4677)
                     /gene="thrS"
                     /locus_tag="ACZPFK_00540"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1404825.1"
                     /translation="MIKVTLKDGVVKEFEAGVSAAEVAKSLGMGLYKAATSARIDGEL
                     ADLRTPLEKDCRLDILTFDDKDGKHAFWHTGAHVMAQAIQHLFPAAQFGIGPALDDGW
                     YYDIGGTEPFTPDQFEAIEAEMKKIVKADLPVEKRFITVEEGRQIFKGQTYKLELLEE
                     YAAKGWQLSVYRQGDFTDLCAGPHLMSTGALKAVKLMSTASAYWRGDAARDSLCRVYG
                     TAFPKAGELQEYLDRLEEAKRRDHRKLGKELGLFAFRDEAPGFPFYLPKGMVLKNTLI
                     DYWREVHKKWDYVEISTPQIMRRTLWETSGHWDHYKDNMYTTVIDEEDFAIKPMNCPG
                     SILVYELEPRSYRDLPLRYGELGIVHRHELSGALHGMFRVRCFTQDDAHILLTKDQIT
                     DEVVRIARLFDEVYSLFGLPYTIELSTMPEDHLGSLEDWEVATNALADAIKSIGKTYE
                     INEGDGAFYGPKLDFHIQDSLGRTWQCGTIQLDYQLPGRFDLEYMGADGQKHCPVMIH
                     RVVFGSIERFIGILTEHFAGAFPTWLAPVQVEVIPIGESHHDYAVRVTEALKAAGVRA
                     EADLRSEKMNYKIREAQLQKIPYMLVVGDKEAAEGTVSVRARKQEKGGVKKLEEFVKA
                     ICGEIAAREN"
     gene            5177..6733
                     /gene="rny"
                     /locus_tag="ACZPFK_00545"
     CDS             5177..6733
                     /gene="rny"
                     /locus_tag="ACZPFK_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1404826.1"
                     /translation="MVSTGVAVLIAVVVGAVAAAIAFSCGIAYRRKVAEQKVGSAEEE
                     AKRIINDAIRTAEQKRKEAAIEAKDEVFKLKAEADKEIKERRSEVTRQERRLDQKEEN
                     IDRKTAALEQKEEEQKKRAELVEARLAEIEQMRERETEKLEHISGLSEEAAKEMLLSR
                     LDEELVHEKALKISAFETQTKEDCDKLAQELITQAVSRCAADHSSEITVSVVPLPSDE
                     MKGRIIGREGRNIRALETATGVDLIIDDTPEAITLSSFDPVRREVARMTLEKLIADGR
                     IHPARIEETVEKCRRELDATMKREGERAVMEVGVHGLHPDLVKLIGRLKYRTSYGQNV
                     LNHSMEVAYLAGLMASELGLNVNMAKRAGLLHDIGKALTHEIEGSHVMIGVDLARKYK
                     ENPAIVHAIEAHHGDVEPQTPLAFIIMAADAISAARPGARRENVENYIKRLEDLEAIS
                     SSFPGVERSYAIQAGREVRIMVKPEQVADDQLTILARDIVKKIEDELEYPGQIKINVI
                     RENRATDYAK"
     gene            7019..7798
                     /locus_tag="ACZPFK_00550"
     CDS             7019..7798
                     /locus_tag="ACZPFK_00550"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR1404827.1"
                     /translation="MKILCVGDVVGTPGLDFLCRRLPGLKRRLSADICIVNGENAHAG
                     GVGITRSLAEDIFAHGADVITTGNHALRRADEALYEETEALLCPANLPHLAPGAGRVT
                     LDLGRCALTVIDLAGVAFLEPVDNPFDTADRLLAGDAARFKVVDIHAESTAEKQALAW
                     YLDGRVSAVFGTHTHVPTADAGVLPGGTGFITDVGMTGPVRSVIGVRPELAVKKQRTH
                     RPVRFEVAEGPCRLDAVLFTCDDASGRCLSAEAVREEEETR"
     gene            7795..8241
                     /locus_tag="ACZPFK_00555"
     CDS             7795..8241
                     /locus_tag="ACZPFK_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HIR1404828.1"
                     /translation="MTELELARLMEGLRDRNAGEGYACLKQLLDESGRTAAVYPHWDE
                     LCALLDEESAYLRTRGLLLLAANARWDAENKLGRDLERLLRYVTDKKPTVARQFLAAL
                     PQVARARPELAPRIREALLCADTSAYPDSTRPLVEKDLRAALAALG"
     gene            8534..10087
                     /locus_tag="ACZPFK_00560"
     CDS             8534..10087
                     /locus_tag="ACZPFK_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1404829.1"
                     /translation="MFHSAPRRAVAALAGLALFAALFSGCGGAPDSSSAVGGGVPAAQ
                     VSGEDTGEFILPYSSADGFNPYLSKSNITLLNSRLLFTNFVDITNDFELEYRAARSVV
                     SSGAEVEITVGGSFADGAAITGADAAASLEAARQSAVFGARFANMGAVRYSGNTVTVT
                     LNRPDSLFAYLLDIPVLKAGETGAASPTSSGRYSVGQDDKGTILTANPDFAQKTALPT
                     IRLVELSGYDALVSGLNIGAISLFSSEQESDLAGSIICNTAYFNLNNLVFLGIQAVGE
                     SSPLAQPALRQAINLAVSRRQIADKAYYSRAYVATGVVNPRFPGEAAAATLAEEADLD
                     AARALIESLGYARDAASGYYQDAAGKRLSFTLLCYSGSSFKRYAATLVAGQLEECGIE
                     VTVQEDGDFASYHDKIASGQAPLYIGEVKLYNNMDMDVFFADGGDAHTGVSASETLLA
                     AYDAMKADISGLAAFEAAFAAEMPFVPLVYKNGVATYSKDFSGLSPTVSDIFYQFERL
                     TIDSTTQKE"
     gene            10093..10443
                     /locus_tag="ACZPFK_00565"
     CDS             10093..10443
                     /locus_tag="ACZPFK_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1404830.1"
                     /translation="MINFYNTFGKVSMTGDYFAGLVSAAASSCYGVADMATSGASDTV
                     KGMIFGSDFPDKGVRVTEDGGKLVIELHIKVTYGLNISAIVKSITHKVKYVVEDATGL
                     QVKKINVSVDDIVS"
     gene            10478..>10761
                     /locus_tag="ACZPFK_00570"
     CDS             10478..>10761
                     /locus_tag="ACZPFK_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1404831.1"
                     /translation="MIKGSVLRDSMISGANSIANQRSQIDELNVFPVPDGDTGTNMSM
                     TIGAAKRDLQGLADGCTVEEASKVAAGSMLRGARGNSGVISSLLFRGFSKA"
BASE COUNT         2026 a         3388 c         3326 g         2021 t
ORIGIN      
        1 catgagcccg gcttatctcg agcgcctctt tgacccgttc agccgcgagg aggacacccg
       61 caccaccgtc accgagggca ccggcctggg catggccatc ccaaagcggc tggtggacct
      121 gctggggggc gagatccgcg tgcagagccg ggagggcgag ggcagcactt tccgggtatc
      181 actgccgctg gagatcgacg aagccccgtc gagggaggcg tcctttcccg gcctgcgggc
      241 gctggtggtg gacgacgggg aggtcacccg cgcctatgtg tgccagaccc tgcgccgcct
      301 cggcgccgag gcggactgta ccgccaccgg agccgaggcc gtgaaaaagg tcgtctcggc
      361 gcagcgggcc ggacagggct acgacgcggt gatcctggac tggaagatgc cgggcatgga
      421 cgggctgcag accgcccggc agctgcggcg cgaggcgccc ggcggtctgc ccatcctgat
      481 catgtccgcc tacaactggg aggaaatcgc cgagcaggcc cgcgccgcgg gcgtcaccgg
      541 atttttgcag aagccgatct tttgttccac cctctgcgag gggctgcgca ggtttgtgct
      601 gggccgcacc gcgcccaaaa aggcggcgcc cggctatgac tttaccggcc ggcggctgct
      661 gctggtggag gacaacgagc tgaaccggga gatcgcggtg gagcttttgc agcaggtcgg
      721 ggcccaggtg gacaccgccg ccaacggcga gctgggggtg gaaaaattcc gcagcgcccc
      781 cgagcatttt tacgcgctca tcctgatgga cgtgcagatg ccggtgatgg acggcctgtc
      841 cgccgcgcgg gtcatccgcg ccctgccccg cggggacgcg gcgcaggtgc ccatcgtggc
      901 catgacggcg gacgcgttcg cagaggacgt ggcggcggca aagcaagccg gcatgagcgc
      961 ccatatcgcc aagcctatcg agctgcagca gctctaccgc accatccagg agctgctgcc
     1021 ctgagaggtg cggcggcagc tgcgccgccg cgccgcaagc ccggtgcacc gctgcttttt
     1081 taccggtcgg cccggcgagg gcggctttgc cgctctccct tgcttttttg cttcgctttg
     1141 caagagttct ctccccggac gggaccaaaa acaaaaaggg caccttcccg gtgccctttt
     1201 ctgtttttga aactatgtag tttaatcgca tcggcttcga cacgattgtt gcaaaataat
     1261 atatttgcta taatgaaaac aatttgtatg gggtggtgta tctgtggcac tatgtattca
     1321 aactccctgt agtttacaat ggaggctgga cgagttagac aaaacaattg attccatgga
     1381 aaacgaaatc gaagaggcga tcacgcagca acaaattcga acaggcaacg actatacgaa
     1441 cattatcctg catacagcgg gaaaatccat tgttagtatg agggaaatta tttacttatc
     1501 cacttttgga tacccggatg gagcattgtc tctcgcaaga aacctatacg agcatgtcat
     1561 aatattggct ttttttgaaa atcacaaata tgacacagat tttgatgact atgtagacga
     1621 ctactatatc gactatgata ttcaacgcaa caaggccttg caatatgaga gtgaaaactg
     1681 cacgaaagat tccgaatcac tgactgttct caaagatgaa ttcgatcggc tcaaacaaac
     1741 agcgcatcat cggatgaccg ggaattactg gtggtcagga agttcaagct ttaatcaact
     1801 tgtgaaagcg gcaatagcat ctattccaga ggagcatgga aagcggtttt tacatggatt
     1861 gcattttgtt tacaaaagag cttgtgtatc tatacattct agttgtattg ggaacacctt
     1921 gagattaggt gctgacccag attttgttgg gatagatact tctcctgcaa taaaaggcca
     1981 ttcgctacca ttgtggtttg caacgacatc gtttgtttac atcatgggtg tcacatatgc
     2041 gttgctcaat cttgactaca aaacacacga ccgtacctta aataatctta ctttgtttct
     2101 ccatcaaaaa gagtcgcaag cctcagatta agtgctgttc catttaaaat attgccgcat
     2161 tctgtgaact ccctacacac ttgtctttac cggccggccc gacgagggcg gccctgccgc
     2221 cctcccttgt ttttttgctt cgctttgccc cagttctcac cccggacggg gcaaaaataa
     2281 aaagggcacc ttaccggtgc ccttttgttt ttggtgaccc gccggagatt cagggaccgg
     2341 ccgcagggcg ggcgcaccgc tgctttttgc cggccggccc ggcgagagca gctttgccgc
     2401 cctcccttgc ttttttgctt cgctttgccg gagttctcac cccgcacggg gcaaaaacaa
     2461 aaagggcacc ttaccggtgc cctttttgtt tttggtgacc cgccggagat tcgaactccg
     2521 gacccattgc ttaaaaggca attgctctgc caactgagct agcgggtcgt tttttctgtt
     2581 ttgcacaatt tgacgcttaa ttattatagc aatcgtcctt tggtttgtca acagttttcc
     2641 ccgttttttc cctctcatcc cgccggtgcg ccaaagcctt ttgcggcacg ggcaaaaaga
     2701 cggacccagc ctgtcggctg ggcccgtctt ttatcctttt tagaaagcct gctccgccac
     2761 ggcgggtgca gctcagttct cgcgggcagc aatctccccg cagatcgctt tcacgaactc
     2821 ctccagcttt ttgacgccgc ccttttcctg cttgcgggcg cgcaccgaaa cggtgccctc
     2881 ggcggcctcc ttgtcgccca ccaccagcat ataggggatc ttctgcagct gggcctcgcg
     2941 gatcttgtaa ttcatcttct cgctgcgcag atcggcctcg gcgcgcaccc cggcggcttt
     3001 cagcgcctcg gtgaccctga ccgcgtagtc gtgatggctc tcgccgatgg ggatgacctc
     3061 cacctgcacc ggggccagcc aggtggggaa cgcccccgcg aaatgctcgg tcaaaatgcc
     3121 gataaagcgc tcgatgctgc cgaagaccac gcggtggatc atcaccgggc agtgcttctg
     3181 gccgtccgcg cccatatatt ccaggtcaaa gcggccgggc agctggtaat ccagctggat
     3241 ggtgccgcac tgccaggtgc ggcccagcga atcctggatg tgaaagtcca gcttggggcc
     3301 gtagaaagcg ccgtcgccct cgttgatctc ataggtcttg ccgatgctct tgatggcgtc
     3361 ggccagggcg ttggtcgcca cctcccagtc ctccagcgag cccagatggt cctccggcat
     3421 ggtggacagc tcgatggtgt acggcaggcc gaacagcgag tagacctcgt cgaacaggcg
     3481 ggcgatgcgc accacctcgt cggtgatctg gtccttggtg agcaggatgt gggcgtcgtc
     3541 ctgggtgaag cagcgcaccc ggaacatgcc gtgcagcgcg cccgacagct cgtggcggtg
     3601 cacgatgccc agttcgccgt agcgcagcgg caggtcgcgg taagagcgcg gctccagctc
     3661 gtacaccaaa atgctgcccg ggcagttcat cggcttgatg gcaaaatcct cttcgtcgat
     3721 gacggtggtg tacatgttgt ccttgtagtg gtcccagtgg ccggaggtct cccacagggt
     3781 gcggcgcatg atctgcgggg tggagatctc cacataatcc cactttttgt ggacctcgcg
     3841 ccaatagtcg atcagggtgt tcttgagcac catacccttg ggcagataga acgggaagcc
     3901 cggggcctcg tcgcggaaag cgaacagccc cagctctttg cccagcttgc ggtggtcgcg
     3961 ccgcttggcc tcttccagcc ggtcgagata ctcctgcagc tcgccggcct tggggaaagc
     4021 ggtgccgtac acgcggcaca ggctgtctct ggcggcgtcg ccgcgccagt aggcgctggc
     4081 ggtgctcatc agctttacgg ctttcagcgc gccggtgctc atcagatggg ggccggcgca
     4141 aaggtcggtg aagtcgccct gccggtagac cgacagctgc cagcccttgg cggcgtactc
     4201 ctccagcagc tccaacttat aggtctggcc cttgaagatc tgccggccct cctccacggt
     4261 gatgaaccgc ttctccaccg gcaggtccgc cttgacgatc tttttcatct ccgcctcgat
     4321 ggcctcgaac tggtccgggg taaagggctc ggtgccgccg atgtcgtagt accagccgtc
     4381 gtccagcgcc gggccgatgc cgaactgggc ggcggggaac aggtgctgga tggcctgcgc
     4441 catcacgtgg gcgccggtgt gccagaacgc gtgcttgccg tccttgtcgt caaaggtgag
     4501 gatgtccagc cggcagtcct tttccagcgg ggtgcgcagg tcggccagct ccccgtcgat
     4561 gcgggcgctg gtggccgcct tgtacagccc catgcccagg ctcttcgcca cctcggcggc
     4621 cgagaccccg gcctcaaact ccttgaccac gccatctttc aatgtgacct tgatcatgcc
     4681 tatctcctcc tgtgtcaact gctgctgtcc cgctgtcttc gttcgttttt gcggcgggcc
     4741 ctctttgggg acaaaacaaa aaggacccat cccgtccggg atgagtcctt gtgacccacg
     4801 gttccacccg cattgcgccg caggggcggc gccgctcgtt tcggggataa cggcccgggc
     4861 cggcgggggt tcgcccccgc gctccgcggc ggtaagccgc cctgtgtcgc cgtgcgggtt
     4921 tccaccctgg ccgacccctg ccgggatcgg cgcagccccc tgctctctgt ccggtcacag
     4981 cgcagcccat gtccgcgtca tcgctttggt agctcaaatt ctacacccgc gcaagaaact
     5041 tgtcaaccgc ccccgtaaat ttcttgacaa ccatcctggc aggtcttaaa ataagaatat
     5101 agtgtatttc aatagtatat cttgatataa aatacattaa attgacaaaa actcccaaaa
     5161 tttaaggagg acccctatgg tatcaaccgg cgtggccgtg ttgatagccg ttgttgtggg
     5221 cgcggtagct gcggctatcg ctttttcttg tggcattgcc taccggcgca aagtggcgga
     5281 gcagaaagtg ggctccgcgg aagaagaagc taaaagaatc atcaacgacg ctatcaggac
     5341 ggcggaacaa aagcggaaag aggccgcgat cgaagcaaag gacgaggtat tcaagctgaa
     5401 agcggaggcc gacaaggaga tcaaggagcg gcgctccgag gtcacccggc aggagcggcg
     5461 gctcgatcaa aaggaagaga acatcgaccg caagacggcc gctctcgagc agaaagaaga
     5521 agagcagaaa aagcgcgcgg agctggtgga ggcaaggctt gccgagatcg agcagatgcg
     5581 cgagcgggag accgaaaagc tggagcacat ctccggcctg tcggaggagg cggcgaaaga
     5641 gatgctgctc tcccgcctgg acgaggagct ggtgcacgaa aaggcgctca agatcagcgc
     5701 ctttgagacc cagaccaagg aggactgcga caagctggcc caggagctga tcacccaggc
     5761 ggtgagccgc tgcgcggccg accactccag cgagatcacc gtctcggtgg tgccgctgcc
     5821 cagcgacgag atgaaagggc ggatcatcgg ccgcgagggc cgcaacatcc gcgccctcga
     5881 gaccgccacc ggcgtcgacc tgatcatcga cgacacgccg gaggcgatca ccctgtccag
     5941 ctttgacccg gtgcggcgcg aggtcgcccg catgacgctg gaaaagctga tcgccgacgg
     6001 gcgcatccat ccggcccgca tcgaggagac cgtcgagaaa tgccgccgcg agctggacgc
     6061 caccatgaag cgggagggcg agcgcgcggt gatggaggtg ggggtccacg gcctgcaccc
     6121 cgatctggtc aagctgatcg gccgcctcaa ataccgcacc agctatggcc agaacgtgct
     6181 caaccactcg atggaggttg cctatctcgc cggcctgatg gcctccgaac tggggctgaa
     6241 cgtcaacatg gccaagcgcg ccggcctgct gcacgacatc ggcaaggcgc tgacccacga
     6301 gatcgagggc agccatgtga tgatcggcgt cgacctggcc cgcaagtaca aagagaaccc
     6361 cgccatcgtg cacgccatcg aggcgcatca cggcgacgtg gagccccaaa ccccgctggc
     6421 ctttatcatc atggccgcgg acgcgatctc ggcggcccgc cccggcgcgc gccgcgaaaa
     6481 cgtggagaat tacatcaagc ggctggagga cctggaggcc atctcctcca gcttccccgg
     6541 cgtggagcgc agctacgcga tccaggcggg ccgcgaggtg cgcatcatgg tcaagcccga
     6601 gcaggtggcg gacgaccagc tcaccatcct ggcccgggac atcgtcaaaa agatcgagga
     6661 cgagctggaa tatcccggcc agatcaagat caacgtcatc cgcgaaaatc gggcgaccga
     6721 ttacgcgaaa taaaactgca aagagcaagg gcgcggagag agcttccgcg cccttgctct
     6781 ttgcttctct ttctctgccg cacagggtgc gccttgtctc ttgctactga tttgaaaaac
     6841 agccgcacag cggctatttt tattccccag cttctccgcc gcttgcgcgg caagcggaac
     6901 gggcggccga aaaaagcacc ccgcgcttca tttttccccc cgcttctgct atgatagggg
     6961 cggaaacaaa aatgccggac gggcaaagcc ttttcccgtg cggcaaacgg aggcgcgtat
     7021 gaaaatcctt tgtgtgggcg acgtggtggg cacgccgggg ctggactttc tctgccgcag
     7081 gctgccgggg ctgaaacggc ggctgtctgc ggatatctgc atcgtcaacg gtgaaaacgc
     7141 ccatgcgggc ggggtgggca tcacccgctc cctcgccgag gacatcttcg cccacggggc
     7201 ggacgtgatc accaccggca accacgcgct gcggcgggcg gacgaggccc tttacgagga
     7261 gaccgaggcc ctgctctgcc cggcaaacct gccgcacctg gcccccgggg caggccgtgt
     7321 gacgctggac ctgggccgct gcgcgttgac ggtgatcgac ctggccggcg tggcctttct
     7381 cgagccggtg gacaaccctt tcgacaccgc cgatcgcctg ctggcggggg acgccgcacg
     7441 cttcaaggtc gtggacatcc acgcggagtc cacggctgaa aagcaggccc tggcctggta
     7501 tctggacggc cgggtgagcg cggtgttcgg cacccacacc cacgtgccca ccgcggacgc
     7561 gggcgtgctg cccggcggca ccggctttat caccgacgtg ggcatgaccg ggccggtgcg
     7621 ctcggtcatc ggggtgcggc ctgagctggc ggtcaaaaag cagcgcaccc accggccggt
     7681 gcgctttgag gtggcggagg gcccctgccg cctggacgcg gtgctcttca cctgcgacga
     7741 cgccagcggc cgctgcctgt cggccgaggc ggtgcgggaa gaggaggaaa cgcgatgaca
     7801 gagctggaac tggcccggct gatggagggc ctgcgggacc ggaacgccgg cgagggatat
     7861 gcctgcctga aacagctttt agacgagagc ggccgcacgg cggcggtcta cccccactgg
     7921 gacgagctgt gcgccctgct ggacgaggag agcgcttatc tgcgcacccg cggcctgctg
     7981 cttttggcgg cgaacgcccg ctgggacgcc gagaacaagc tggggcgcga cctggagcgc
     8041 ctgctgcgct atgtgaccga taaaaagccc accgtggccc ggcagttcct cgccgcgctg
     8101 ccgcaggtgg cccgggcccg gccggagctg gccccgcgca tccgggaggc gctgctctgc
     8161 gccgacacct cggcctaccc cgacagcacc cgtccgctgg tggaaaagga cctgcgggcg
     8221 gcgctggccg cgctaggctg accgcccgcc gccttgcctt tttgcaacgt ctcccgcgac
     8281 cggccctatg ctcggctccc atgcccggcc cctatggccg aaccctatgt ccggcctcca
     8341 gggccggcct cggcgcccgc cctgccggcg gcgggcaaaa ctgaactttc ccttttcttt
     8401 gaccgcaaaa aggggtataa tgttcgggtc aaacggccac gggcctcttt tgcgggcctc
     8461 ctgcggccgg gggctttcct gggccccatc atccgcccgg cctttttctc cccgccgggc
     8521 agggaggttt cacatgtttc actctgcacc tcggcgggct gtcgccgcac tggccggcct
     8581 cgcccttttt gccgcgctgt tctccggctg cggcggcgcg ccggacagct cctccgccgt
     8641 cggcggcggc gtgcccgccg cccaggtctc gggggaggac acgggcgaat tcatcctgcc
     8701 ctattccagc gccgacggct tcaaccccta tctctccaag agcaacatca cgctgctgaa
     8761 ctcgcggctg ctgttcacga acttcgtgga catcaccaac gacttcgagc tggagtaccg
     8821 ggccgcgcgc tcggtggtgt ccagcggcgc cgaggtggag atcacggtgg gcggcagctt
     8881 tgccgacggc gccgccatca ccggcgccga cgcggcggcc agcctggagg ccgcgcggca
     8941 gagcgcggtt tttggcgcgc gctttgccaa tatgggcgcg gtgcgctaca gcggcaacac
     9001 ggtgaccgtg acgctgaacc ggccggacag cctgttcgcc tacctgctgg acatcccggt
     9061 gctcaaggcc ggggagacgg gcgccgcctc ccccacctcc agcgggcgct attcggtggg
     9121 gcaggacgac aaggggacga tcctcaccgc caacccggat tttgcccaaa agaccgccct
     9181 gcccaccatc cgcctggtgg agctgagcgg ctacgacgcg ctggtctccg gcctcaacat
     9241 cggcgccatc agcctttttt cctctgagca ggagtccgac ctggcgggca gcatcatctg
     9301 caacaccgcc tatttcaacc tgaacaatct ggtctttctc ggcatccagg ccgtgggcga
     9361 gtcgtcgccg ctggcccagc ccgcgctgcg gcaggccatc aacctggccg tgtcccgccg
     9421 ccagatcgcc gacaaggcct attactcgcg cgcctatgtg gccaccggcg tggtcaaccc
     9481 gcgctttccg ggcgaggccg cggcggccac gctggccgag gaggccgacc tggacgccgc
     9541 ccgggcgctg atcgaatcgc tggggtacgc cagggacgcc gccagcggct actatcagga
     9601 cgccgcgggc aaacgcctct cgttcacgct gctgtgctac tcgggcagct cgttcaagcg
     9661 ctatgccgcc actttggtgg ctgggcagct ggaggagtgc ggcatcgagg tcacggtaca
     9721 ggaggacggc gactttgcca gctatcacga caagatcgcc tccgggcagg cgcctctgta
     9781 catcggggag gtaaagttgt ataataatat ggatatggac gtctttttcg ccgacggcgg
     9841 ggacgcccat accggcgttt cggcctccga gacgctgctc gcggcctacg acgcgatgaa
     9901 agccgacatc tcgggcctgg cggcctttga ggccgctttc gcggcggaga tgccctttgt
     9961 gcctctggtc tacaaaaacg gcgtcgccac ctacagcaag gatttttcgg gcctttcgcc
    10021 cacggtcagc gatatcttct accagtttga gcgtctcacc atcgacagca ccacccaaaa
    10081 ggagtgaaca gcatgatcaa cttttacaac accttcggca aggtcagcat gaccggcgac
    10141 tacttcgcgg gcctggtctc agccgcggcg agcagctgct acggcgtggc cgacatggcg
    10201 accagcggcg cttcggacac ggtcaaaggc atgatcttcg gctccgattt tcccgacaag
    10261 ggcgtgcggg tgaccgagga cgggggcaag ctcgtcatcg agctgcacat caaggtgacc
    10321 tacggcctca acatctcggc catcgtcaag agcattaccc ataaagtcaa atacgtggtg
    10381 gaggacgcca ccggcctgca ggtaaaaaag atcaatgtct cggtagacga catcgtctcc
    10441 tgacggaaat tagacagatt aggggagtat acgcaagatg attaaaggtt ctgttttgag
    10501 ggacagcatg atcagcggcg ccaacagcat cgccaaccag cgcagccaga tcgacgagct
    10561 gaacgtcttt ccggtgccgg acggcgacac cggcaccaac atgagcatga ccatcggcgc
    10621 ggcgaagcgc gacctgcagg ggctcgccga cggctgcact gtggaggagg ccagcaaggt
    10681 cgccgcgggc agcatgctgc gcggcgcgcg cggcaacagc ggcgtcatct cgtccctgct
    10741 gttccgcggc ttttccaagg c
//
LOCUS       DBMDYA010000010        10582 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1749_length_10582_cov_1.499094, whole genome shotgun sequence.
ACCESSION   DBMDYA010000010 DBMDYA010000000
VERSION     DBMDYA010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_10582_cov_1.499094"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="ACZPFK_00575"
     CDS             complement(<1..1239)
                     /locus_tag="ACZPFK_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1404832.1"
                     /translation="MRIATGVYQNRGSGGFLQFLRQEKTPFFQTEQRPDCPVCLFERE
                     FPDWGYDYVESGGVAFVCGADKHTFAFDSGYLTRASLEFADFSFCGQGNARVLALAGI
                     FEGRGVGLLTTHENRASIHGRHPARYPVLLEKKLGAGALFYTGLPLTALLTAEGSTLR
                     RTSDLSDFDERIASVDKDKLEAALRWILRRCFRAAGAPYVHLWYYPDGARNVFAYRLD
                     GDGMAEPGTQNLMRAAKEARCPFTLYVSGEFCAGNREEERKIAEFGQYNEIGCHNFVH
                     DAMDSLEENLDSLGRFERWMEEIGVPFQRVFASPRGMYGVNMARALVAKGYRHSSDFS
                     LKAGGFPFYPVLDGEPFGPLQIPVDAFNVCRLWVQCGELRREKAGAEEILSRYQALAD
                     QKLSQGTPLLFFCHPQYFGAV"
     gene            complement(1407..1796)
                     /locus_tag="ACZPFK_00580"
     CDS             complement(1407..1796)
                     /locus_tag="ACZPFK_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014009.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR1404833.1"
                     /translation="MLTRKKVVLPAAGFADLTDWVAECAAACGDEGLCIVQALDPRAG
                     IVIGDGDPRAQKDLLDDMERAFPERASYESADGPARAAAAAKSAVFGAEKWLPVAGGA
                     LLLGESQRVLAVSYSGEKELELAVATV"
     gene            complement(1790..3016)
                     /locus_tag="ACZPFK_00585"
     CDS             complement(1790..3016)
                     /locus_tag="ACZPFK_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1404834.1"
                     /translation="MTKQELDSKLRRQALSYPQVARAQVEGFRRGMREASFPPDEVLR
                     DIRHIYITGSGDCNAAAAICKPVVEKLLGGFSAKVYVDTPLHVGRYLSLVASDDNPAV
                     AAQTLLIGLSVWGFPARVVECLRRAEKLGVHTLDITNNVDSPVAEASEYLLDVHVPET
                     PDTHPGCRDYFGTLVGTVLTAAYIGEVKGLQPAGTLDRLADEIIRMADEYAARMEALD
                     DAMFRLAEEVRDKVDRFEGVGDGYEYASAFFFPAKIIETVGNYATWTDSIHFRKNSLY
                     YREPGRIFTAFYGEVDAANRDSIAEAAKLAQAAGREVLFLGDAPGAAFGLPPEVRALE
                     LPKAPAEWPELAAFFNHLPTDLLSAYLCEMWGGHYFRDGSSGSFTAGEVTTIWGQPGM
                     STLKGSKIVIVEEEQC"
     gene            complement(3036..3839)
                     /locus_tag="ACZPFK_00590"
     CDS             complement(3036..3839)
                     /locus_tag="ACZPFK_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR1404835.1"
                     /translation="MTRTFELETARDGWHVIDAQVRQAVAESGVKDGICLLHLPHTTA
                     AVAATSSWDPKGIEDSIRDIKAKFPARTSYAHPYSPFTSAARSRAALAGCGRTFIVRD
                     GALLLGHSQTLLIYEFDGPQLRSFSVTVLPRPFYFDTAAFKSSFGEMRDVTAEVESIV
                     RRSGVQDGFCHVTVVAATAGLMLCAAGEDAQADIWGDIERLIPTRADFHHRETASDAA
                     GHTKTFVAGTQLDLPVQGGALVLGKDQRVVYAEFDGPRPRDIKVAVYAD"
     gene            complement(3836..4930)
                     /locus_tag="ACZPFK_00595"
     CDS             complement(3836..4930)
                     /locus_tag="ACZPFK_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404836.1"
                     /translation="MDKAKSRNRYRSFAEGLPALCGELLDRLWMSDALQEALSVGSCF
                     DARKVVLTGSGVGYAAVLAGAPVFSEQCDIFFGAEVMAQADFNYFNNVAAMGIGEPNT
                     PLVVVATEQADDPDAARTLEMVAQAGANSLLLCAAAQSAQAPLARSALCLGLDEAAPD
                     FLPRAYLALTLALVCAGYRIARVRGTVSELELQRVRQALLDYTAAMSGCLEAAQAAAQ
                     DFAQRCGQRDRFDYIADAEARAAAHLLGTVGARTLGYQYAVNDSEEWCHVNYWMEPRF
                     DLCTVMWGLRAQPSFSRTVETMGCVHKLERPYLFLTDAGEDEFLAGTRCCAIPAPPQG
                     RRWMAQLVSFLPGLLALGELEQNERGGAGR"
     gene            5228..6172
                     /locus_tag="ACZPFK_00600"
                     /pseudo
     CDS             5228..6172
                     /locus_tag="ACZPFK_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            6333..7277
                     /locus_tag="ACZPFK_00605"
     CDS             6333..7277
                     /locus_tag="ACZPFK_00605"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1404837.1"
                     /translation="MSHKPIPVLIDCDTGIDDANALVIACASGRLDIRAVTTVAGNTV
                     IENSTRNTLNVLHLLGRDDIPVGRGAAGPIFGPLKAAASTHGEDGLGGYRFERDATAA
                     LSPLPAPELIARTLEASGEPVTLIVLGPATNIAALLLQRPDLHAKIEKIVFMGTSDQR
                     SLFRTFNIRVDPEAFRICLESGLPVYGVGRLLALQHAYITQDELEEIGRIGNRVGDFV
                     YRILSAYTDYRLVDGKKVIAMFDEATVAFTAAPELFTCQKYFCEVDCGNDWKRGLTYI
                     DFDGNLKKAEEEKNLYYVETADREGFVKLMLDAIRSYG"
     gene            7340..8836
                     /locus_tag="ACZPFK_00610"
     CDS             7340..8836
                     /locus_tag="ACZPFK_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR1404838.1"
                     /translation="MKLDFEYGQGLMSAELPDSTDVFIPGETVPDPPCLPQDWDSLYS
                     ATLQSIRSPIGMPTLTQLAHKGSTVVFVIPDIVKGGNQPTSHRKVAIRACLDELYSVG
                     VEKKDILLLFSNGLHPRATVPEMKTILGEELFGEFYPSGQITSHDSEDYEHLVDLGFT
                     SHGDHVIMNKYVYDADIAILIGHTQGNPYGGYSGGYKHCATGISHWRSISAHHVPQVM
                     HRPDFVPVSTSSEMRHKFDQQGMFMEEKMGKKFFCCDAVLDTRSRQIEINAGWAKEMQ
                     PISWKTADKRTYVHWAEKKYDVVVFGMPTNFRYGNGMGTNPIQMMQALSAQVIRHKRV
                     LADHCVFIVSSICDGWFHEERWPYLKELYEMFQHDSMNILPDMNRYGEYFATNEEYIR
                     KYRFANAFHPFHGFSMMSCGHLAEEHTSAIYIVGAREPGIARGMGLKTRATFEEALAD
                     AKKKFVGENPNILALPQTFTTAAVHLCMKDPSQNSRTRDSAPAHPCGG"
     gene            8891..9727
                     /locus_tag="ACZPFK_00615"
     CDS             8891..9727
                     /locus_tag="ACZPFK_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1404839.1"
                     /translation="MAYIFLNLKRFDITPERGGVNRIAPPAGWAEAIVERTLAINSWP
                     QDEFVSFFPEAHLLAAAAAKRETGSRMAVGCQGCAGADTAVGGNFGAFTAQLTANSAA
                     GLGCEWALIGHCEERAAKAGILAAGGGDAAAVDALLNAEVLRAQEAGLRVLFCIGEKH
                     EELDRWQEVLRTQLTRGLQGADLSRVVIAYEPIWAIGPGKTPLGRERIQETARFIRSV
                     AELPVVYGGGLKLDNAGMLATIPEIAGGLIALTRFSGEIGFYPDEYVEIARAYLEEKN
                     KH"
     gene            10005..>10582
                     /locus_tag="ACZPFK_00620"
     CDS             10005..>10582
                     /locus_tag="ACZPFK_00620"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1404840.1"
                     /translation="MELTKKRELQRFARQLRIDALNEVHDAHSGHIGGSLSICDTLAY
                     LYKRVLRIDPAHPHDPGRDRFVLSKGHASPALYAVLAEMGFFPREELKSFRRIGCMLQ
                     GHPDMKKIPGVDMTSGSLGQGVSAAVGMAVAGKITGADYRVFAMLGDGELQEGQVWEA
                     AMLAAHRGLDNLTAIVDNNGLQIDGPVDKVNSP"
BASE COUNT         1876 a         3692 c         3286 g         1728 t
ORIGIN      
        1 gaccgcgccg aaatactgag gatggcaaaa aaagagcagc ggcgtgccct gtgagagctt
       61 ttgatcggcc agcgcctgat agcggctgag gatttcctcc gcgcccgcct tttcccgcct
      121 cagttccccg cactgcaccc acagcctgca cacgttgaac gcgtccacag ggatctgcag
      181 cgggccgaac ggctccccgt ccagcacggg gtaaaagggg aagccgcccg ccttcaggct
      241 gaagtcggag gagtggcgat atcctttcgc cacgagcgcg cgggccatat tgacgccgta
      301 catgccgcgc ggggaggcga acacccgctg gaacggcacg ccgatctctt ccatccagcg
      361 ctcaaagcgc ccgaggctgt ccagattttc ctctaggctg tccatggcgt cgtggacaaa
      421 gttgtggcag ccgatctcgt tatactgccc gaactcggcg atcttgcgct cctcctcgcg
      481 gttgccggcg caaaattccc ccgagacata cagcgtaaag gggcaccggg cctcttttgc
      541 cgcgcgcatc aggttttggg tgcccggctc cgccataccg tccccatcca ggcggtaggc
      601 aaagacgttt ctcgccccgt ccgggtaata ccacaggtgg acatacggcg cgccggcggc
      661 ccgaaagcag cggcggagga tccatcgcag cgccgcctcc agcttgtcct tgtcgaccga
      721 ggcgatgcgc tcgtcaaagt cggacaggtc gctggtccgc cgcagggtgc tgccctccgc
      781 ggtcaaaagc gctgtgagcg gcaggccggt gtaaaaaagg gcgcccgcgc ccagcttttt
      841 ctccagcagc acgggatagc gggccgggtg gcggccgtgg atgctggccc ggttttcatg
      901 ggtggtcagc aggccgaccc ctctcccctc gaaaatgccc gccagagcca agacccgcgc
      961 gtttccctgc ccgcaaaagg aaaaatcggc aaactccagg cttgccctgg tcaggtagcc
     1021 gctgtcaaag gcaaaggtgt gcttgtccgc gccgcagaca aacgccacgc cgccgctctc
     1081 cacatagtca tagccccagt ccgggaactc ccgctcaaac aggcagaccg ggcaatccgg
     1141 tctctgctcc gtctgaaaaa agggcgtctt ctcctggcgc aggaactgta aaaagccgcc
     1201 gctgccgcgg ttttgataga ccccggtcgc aatcctcatt ttttgcctcc ccattgcttt
     1261 ttaaaagcgt tccgtcggcg cccccccaca gggccgccgc aaaaaagggc gctgcggaag
     1321 ccttgccccg cagcgccctt tttccgttgc cgacaccggt cgatgccggt ttctcccgcc
     1381 gcgccgccga gccgccttga aagcgctcag accgtggcga cggccagctc cagctctttc
     1441 tcgccgctgt aagagacggc cagcacgcgc tggctttcgc ccagcagcag cgcgccgccc
     1501 gccaccggca gccatttttc agccccgaac accgcgcttt tggcggcggc agccgcccgc
     1561 gcgggcccgt cggcgctctc atagctcgcc cgctcgggga aagcccgctc catatcatcc
     1621 aacagatcct tttgcgcccg cgggtccccg tccccgatca cgatccccgc gcgcgggtcc
     1681 agcgcctgga cgatgcacag cccctcgtcc ccgcaggccg ccgcacactc cgccacccaa
     1741 tccgtcagat cggcaaaacc ggcggcgggc aaaacaactt tttttcttgt cagcattgtt
     1801 cttcctccac gatgacgatc ttgctgcctt tcagggtgct catgcccggc tggccccaga
     1861 tcgtggtgac ctctccggcg gtgaaagagc cgctggagcc gtcgcggaaa tagtggcctc
     1921 cccacatctc gcacaggtag gcgctgagca ggtcggtggg caggtggttg aagaaagccg
     1981 ccagctccgg ccactcggcg ggcgccttgg gcagctccag cgcgcgcacc tccggcggca
     2041 gcccaaacgc cgcgcccggc gcgtcgccca ggaacagcac ctcccggccg gccgcctggg
     2101 ccagcttcgc cgcctcggcg atgctgtccc ggttggccgc gtccacctct ccgtaaaagg
     2161 cggtgaagat gcggcccggc tctcggtaat agaggctgtt cttcctgaaa tggatgctgt
     2221 cggtccaggt ggcgtagttc cccaccgtct cgatgatctt ggcggggaag aagaacgcgg
     2281 aggcgtattc atagccgtcg cccacgccct caaagcggtc caccttgtcc cggacctcct
     2341 cggcaaggcg gaacatggcg tcgtccagcg cctccatgcg ggcggcgtat tcgtccgcca
     2401 tgcggatgat ctcgtccgcc agccggtcca gggttccggc gggctgcagc cctttcacct
     2461 cgccgatata agccgccgtc agcacggtgc ccaccagggt gccgaagtaa tcccggcagc
     2521 ccggatgggt gtccggcgtc tcggggacgt gtacgtcgag gaggtattcc gacgcctcgg
     2581 caaccgggga gtccacgttg ttggtgatat ccagcgtgtg gacgcccaat ttttccgcgc
     2641 gccgcaggca ctccaccaca cgggcgggga agccccacac gctcaggccg atcagcagcg
     2701 tctgggccgc cacggccggg ttgtcgtccg aggccaccag cgacagatac cgccccacgt
     2761 gcagcggcgt gtccacatag accttggccg aaaagccgcc cagcagcttt tccacgacgg
     2821 gcttgcagat ggcggccgcc gcgttgcagt ccccgctgcc ggtgatatag atatgccgga
     2881 tgtcccgcag cacctcgtcg ggcgggaaag acgcctcccg catgccccgg cggaagccct
     2941 ccacctgcgc ccgcgcgacc tgcgggtagc tcagcgcctg acggcgcagc ttcgaatcca
     3001 attcctgttt tgtcatcttc tgtcctcctc tctcctcaat cggcatagac ggcgaccttg
     3061 atgtcccggg ggcgcggccc gtcgaattcc gcgtagacga cccgctggtc cttgcccagc
     3121 accagcgccc cgccctgcac cggcaggtcc agctgggtgc cggccacaaa ggtcttggtg
     3181 tggcccgcgg cgtccgacgc cgtctcccgg tggtggaaat ccgcccgggt ggggatcagc
     3241 cgctctatat ccccccagat atccgcctgc gcgtcctccc cggcggcgca gagcatcagg
     3301 cctgcggtgg ccgcgaccac cgtcacatgg cagaagccgt cctgcacgcc gctgcgccgc
     3361 acgatgctct ccacctctgc ggtgacatcc cgcatctcgc cgaagctgga cttaaaggcg
     3421 gccgtgtcga aatagaacgg gcgcggcagc acggtgaccg aaaagctgcg caactgcggc
     3481 ccgtcgaact catagatcag cagcgtctgc gagtgcccca gcagcagcgc gccgtcccgc
     3541 acgataaagg tgcggccgca gccggccagc gccgcccggg aacgggcggc ggaggtgaaa
     3601 ggggaatagg ggtgtgcgta ggaggtgcgg gccgggaact tcgccttgat gtcccggatc
     3661 gaatcctcga tgcccttggg gtcccaggag gaggtggcgg cgaccgccgc ggtggtgtgg
     3721 ggcagatgca gcaggcagat gccgtctttc acgccgctct cggcgaccgc ctggcgcacc
     3781 tgcgcgtcga tcacatgcca gccgtccctc gccgtttcca gctcaaatgt ccgcgtcatc
     3841 tgcccgcacc tccccgctca ttttgttcca gctcgcccag cgccagcagc ccgggcaaaa
     3901 agctgaccag ctgcgccatc cagcggcggc cctgcggcgg cgcggggatg gcgcagcagc
     3961 gggtgcccgc caaaaactcg tcctcgcccg catcggtgag gaacagatag ggacgctcca
     4021 gcttgtggac gcagcccatc gtctccacgg tgcgggagaa agacggctga gcgcgcaggc
     4081 cccacatgac ggtgcacagg tcaaagcgcg gctccatcca gtaattgacg tggcaccact
     4141 cctcgctgtc gttcaccgcg tactggtagc ccagcgtgcg ggcccccacc gtgcccagca
     4201 ggtgggcggc ggcgcgcgcc tcggcgtcgg cgatgtaatc gaaccggtcc cgctgcccac
     4261 agcgctgcgc aaaatcctgc gccgcggcct gcgcggcctc caggcagccg gacatggccg
     4321 ccgtgtagtc cagcagcgcc tgccggacgc gctgcagctc cagctcggag accgtgcccc
     4381 gcacccgggc gatgcggtag ccggcacaga ccagcgccag cgtgagggcc agataggcgc
     4441 gcggcagaaa atccggcgcc gcctcgtcca ggcccaggca gagagcgctg cgggccagag
     4501 gggcctgggc gctctgcgcc gccgcgcaga gcagcaggct gttggccccc gcctgcgcca
     4561 ccatctccag cgtgcgggcc gcgtccgggt cgtccgcctg ctcggtggcc accaccacca
     4621 gcggcgtgtt cggctcgccg atgcccatgg ccgcgacgtt gttgaaatag ttgaaatccg
     4681 cctgcgccat cacctcggcg ccgaaaaaaa tatcgcattg ctccgaaaag accggcgcgc
     4741 ccgccagcac ggcggcgtag cccacgccgc tgccggtcag cacgactttg cgggcgtcaa
     4801 agcagctgcc cacgctcagc gcctcctgca gcgcgtcgct catccacagc ctgtccagca
     4861 gctcgccgca aagggcgggc agcccctctg caaacgagcg gtaccggttc cggctttttg
     4921 ccttgtccat aaaccatcct cctttttgtc ccgatgaatt cacggcgggc ccgtccagcg
     4981 cccccttttg ccggggccgc tgcgcgggcg cgtcttcctt ttccactata acaagttcgg
     5041 cacaaaaagt attttcattt taagcaaacc ttttcatgcg cctcctgtcc gaggctgccg
     5101 caggggggat cagccctgtt ttttcggccc ccgcgcaccc ttgtgaaaac gattggctta
     5161 acagtttccc acccgtctgg aaaatgcggt agaatggaat cgagcttctg agaaacgagg
     5221 tgttttgatg tccgcgagca ccattcccgt cattatcgac tgcgataccg gcatcgacga
     5281 cgccaacgcg ctggtgctgg cctgcgccag cccccggctc aatatcctgg ccgtaaccac
     5341 ctccgcgggc aacaccgtga tcgaggccgt gacgcaaaat accctcaatg tatgacatct
     5401 gctgggccgg gacgacatcc cggtgggccg gggcgcggcc cgtcccctgc tgggggcgcc
     5461 gctctccgcc gagcacgtcc acggcgccaa cgggctgggg ggatatcagt tcgcgcggga
     5521 tgtctccgac gcgctctccc ccctgccggc cggcgcattg atggcccaca tactggaggc
     5581 tcacaccgag ccggtcaccc tgatcgcgct gggccgcgcc accaacatcg ccgccctgct
     5641 ggcgctgcgg cccgacctgc gcgccaagat cgaaaagatc gtctttatgg gcaccgccga
     5701 ccagcacagc ctggttcgca ccgccaatgt ccgcggcgac ccggaggcat tccagatctg
     5761 cctggacagc gggctgcccg tgtacggcgt cggtcgcctg ctggcgctgc ggcacgccta
     5821 cctcacccag aacgagctgg aggagatcgg ccgcatcggc acaaaggtgt cgggctttgt
     5881 ttaccagacg ctttccgcct attccgacca tcagctggtg gacggcaaaa atacgattcc
     5941 catgtttgac gaggccacgg tcgcctttgc catcgacccg gggctgttta cttatcagaa
     6001 atatttctgc gaggtggact gcggccacgg ctggcggcgc ggcctgacct acatcgactt
     6061 agacggcacc ctcaaaaagc ccgaggtgga aaagaacctt tactacgtgg agagcgccga
     6121 ccgcgcgggc tttgccaagc tgatgctgga cgccgtgcgc ttctatgggt gagcagggcc
     6181 gccaaagccc cgctgcgccg ttttaaaaac cgtgcaaagc gagtgggatg cgaaaggcat
     6241 cccagctccg gctgcaaatc agatcccgcc ggcaaagggt ggttcggctt ttcgcgcccc
     6301 tttctgccgg atcaagagat ggaggtaatt tgatgtccca caaacccatt cccgtcctca
     6361 tcgactgcga taccggcatc gacgacgcca acgcgcttgt catcgcctgc gccagcgggc
     6421 ggctggatat ccgcgccgtc accacggtgg ccggcaacac cgtgatcgag aactcgaccc
     6481 gcaacacatt gaacgtcctg cacctgctgg gccgggacga catccccgtg ggccggggcg
     6541 ccgccgggcc gatcttcggg ccgctgaaag ccgccgcctc cacccacggc gaggacggcc
     6601 tgggcggcta ccgctttgag cgggacgcca ccgcggcgct gtcccccctg cccgcgccgg
     6661 agctgatcgc ccgcacgctg gaggcctccg gcgagccggt caccctgatc gtgctgggcc
     6721 cggccaccaa catcgccgcc ctgctactgc agcgccccga cctgcacgca aagatcgaaa
     6781 agatcgtctt tatgggcacc tcggaccagc gcagcctatt ccgcaccttt aatatccggg
     6841 tggacccgga ggcgttccgc atctgcctgg aaagcgggct gccggtgtac ggcgtgggcc
     6901 gcctgctggc gctgcagcac gcctatatca cccaggacga gctggaggag atcggccgca
     6961 tcggcaaccg ggtgggggat ttcgtctacc ggatcctctc cgcctacacc gactaccggc
     7021 tggtggacgg caaaaaggtg atcgccatgt tcgacgaggc caccgttgct ttcaccgccg
     7081 cccccgagct gttcacctgt caaaaatact tctgcgaggt ggactgcggc aacgactgga
     7141 aacgcgggct gacctatatc gacttcgacg gcaacctgaa aaaggcggag gaggagaaaa
     7201 acctctacta cgtggagacc gccgaccgcg agggctttgt caagctgatg ctggacgcca
     7261 tccgctctta cgggtgaacg gagccctcca gcccgccccg atgttttgac ccggaaagtt
     7321 tggaaagtga ggaaccgaca tgaaattgga ttttgaatac ggacagggcc ttatgagcgc
     7381 ggagctgccg gacagcaccg acgtctttat ccccggcgag acggtgccgg acccgccctg
     7441 cctgccgcag gactgggaca gcctgtacag cgccacgctg cagagcatcc gcagccccat
     7501 cggcatgccc accctgaccc agcttgccca caagggcagc accgtggtct tcgtcatccc
     7561 cgatatcgtc aagggcggca accagcccac cagccaccgc aaggtggcca tccgcgcctg
     7621 tctggacgag ctgtacagcg tcggggtgga gaaaaaggat atcctgctgc tcttctccaa
     7681 cggtctgcat ccccgcgcca cggtgccgga gatgaagacc atcctgggcg aggagctgtt
     7741 cggcgagttc tatccctccg gccagatcac cagccatgac tctgaagact acgagcatct
     7801 ggtggacctg ggctttacca gccacggcga ccacgtcatc atgaacaaat acgtctatga
     7861 cgccgacatt gccatcctca tcggccacac ccagggcaac ccctacggcg gctactccgg
     7921 cggctacaag cactgcgcca ccggcatcag ccactggcgc agcatcagcg cccaccacgt
     7981 gccccaggtg atgcaccggc cggactttgt gcccgtgtcc accagcagcg agatgcgcca
     8041 caagttcgac cagcagggca tgttcatgga agagaagatg ggcaaaaagt tcttctgctg
     8101 cgacgcggtg ctggacacaa ggtcccgcca gatcgagatc aacgccggct gggcgaaaga
     8161 gatgcagccc atcagctgga aaacggccga caagcgcacc tacgtgcact gggccgagaa
     8221 aaagtacgac gtggtggtgt tcggcatgcc caccaatttc cgctacggca acggcatggg
     8281 caccaacccc atccagatga tgcaggcgct ctccgcccag gtcatccggc acaagcgggt
     8341 gctggcggac cactgcgtct tcatcgtctc ctccatctgc gacggctggt tccacgagga
     8401 gcgctggccc tacctgaaag agctgtacga gatgttccag cacgactcga tgaacattct
     8461 gccggacatg aaccgctacg gcgagtattt tgccaccaac gaggagtaca tccgcaagta
     8521 ccgctttgcc aacgcgttcc acccgttcca cggcttttcg atgatgagct gcggccacct
     8581 ggcggaggag cacaccagcg ccatctacat cgtgggcgcc cgcgagccgg gcatcgcccg
     8641 cggcatgggc ctcaagaccc gcgccacctt tgaggaggcg ctggcggacg cgaagaaaaa
     8701 atttgtgggg gagaacccca atatcctggc cctgccccag acctttacca ccgccgcggt
     8761 gcacctgtgc atgaaagacc ccagccagaa cagccgcacc cgcgacagcg cgcccgcgca
     8821 cccctgcggc ggctgaggca cggcggcggg cccacatcaa acaagacagg ataaggagcg
     8881 gcaaaccatt atggcataca tctttctcaa tctgaaacgg ttcgacatca cccctgagcg
     8941 gggcggggtc aaccgcatcg cgccccccgc cgggtgggcg gaggcgatcg tcgagcgcac
     9001 gctggcgatc aacagctggc cgcaggacga gttcgtctct ttcttccccg aggcgcacct
     9061 gctggcggcc gcggcggcaa agcgggagac gggcagccgc atggcggtgg gctgccaggg
     9121 ctgcgccggc gccgacaccg ccgtgggcgg caatttcggc gcgttcaccg cccagctcac
     9181 cgccaacagc gcggcgggcc tgggctgcga gtgggcgctg atcggccact gcgaggagcg
     9241 ggccgccaag gcgggcattc tcgccgccgg cggcggcgac gccgcggccg tggacgcgct
     9301 gctgaacgcc gaggtgctgc gcgcccagga ggcgggcctg cgggtgctct tctgtatcgg
     9361 tgaaaagcat gaggagctgg accgctggca ggaggtgctg cgcacccagc tgacccgcgg
     9421 gctgcagggg gcggacctgt cccgggtggt gatcgcctat gagcccatct gggccatcgg
     9481 ccccggcaag acccctctcg gccgcgagcg catccaggag accgcgcgct tcatccgcag
     9541 cgtggcggag ctgcccgtcg tctacggcgg cggcctcaag ctggacaacg ccggcatgct
     9601 ggccaccatc cccgagatcg cgggcggcct gatcgccctg acccgttttt ccggcgagat
     9661 cggcttttac ccggacgagt atgtggagat cgcccgcgct tatctggagg aaaaaaacaa
     9721 gcactgaccc caccgcaggg acgaaagaga gggcggcgcg gcaggccgtt cccgccgtgc
     9781 agcgggcgcg ccgccgcgcc gctttttgcg cagcttcggc aggggacggt cgtctgaccc
     9841 cagcaaaaaa ggcccgtccg ccgcgtcggc ggcgctgggg gccccagctg cctgcggcgc
     9901 cccgcttttc cgcggccgga tgtttttttg cgggcaggcg acgcccccgg cctcaccgcg
     9961 cttttcccct ctcacttttt cccgcaacat aaaaaggagg cattatggag ctgacgaaaa
    10021 aacgcgagct gcagcgcttt gcgcggcagc tgcgcatcga cgccctgaac gaggtgcatg
    10081 acgcgcactc ggggcacatc ggcggatcac tctcgatctg cgacaccctg gcctatctct
    10141 acaagcgggt gctgcgcatc gaccccgccc atcctcacga cccgggccgg gaccgctttg
    10201 tcctctcgaa aggccacgcc agcccggccc tttacgccgt gctggccgag atgggctttt
    10261 tcccgcggga ggagctgaaa agcttccgcc gcatcggctg tatgctgcag gggcacccgg
    10321 acatgaaaaa aatccccggc gtggacatga cctccggctc gctgggccag ggcgtgtcgg
    10381 ccgccgtggg catggcggtg gcgggcaaga tcaccggcgc ggactaccgc gtcttcgcca
    10441 tgctgggcga cggcgagctg caggagggcc aggtgtggga ggccgcaatg ctggccgcgc
    10501 accgcgggct ggacaacctg accgccatcg tggacaacaa cggcctgcag atcgacggcc
    10561 cggtggacaa ggtgaactcg cc
//
LOCUS       DBMDYA010000011         9865 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1888_length_9865_cov_1.560836, whole genome shotgun sequence.
ACCESSION   DBMDYA010000011 DBMDYA010000000
VERSION     DBMDYA010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_9865_cov_1.560836"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="ACZPFK_00625"
     CDS             complement(<1..857)
                     /locus_tag="ACZPFK_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR1404841.1"
                     /translation="MKKEKIGVIGAGHVGSHVASALSLGGLCDEIVLVDCDADKARAQ
                     AIDLADAAVCFPRPVAIAAGGYDDLADAALVVMAACDGILEEDRLLELDNTVRVAQQI
                     LPRLKGSGFCGVVVSITNPCDIIAQYLAAGTGLTVIGTGTALDSARLRLRIAQAAGVA
                     PASVEAYCLGEHGDNQVAALSSAVIGGRPVQELCSEAALAEAARCTISAGWEIAAPKG
                     STEFGIGSVAAALCAAILRDERRVIPCSAMLNGEYGERGVYASLPRVIGAAGVLRTLT
                     PELNPAERE"
     gene            complement(869..2023)
                     /locus_tag="ACZPFK_00630"
     CDS             complement(869..2023)
                     /locus_tag="ACZPFK_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1404842.1"
                     /translation="MQKKFVSKKYENGHATMLGDLGPKAAAYEDLINFSIGDPDLKTD
                     DRIIEAAFADAKAGYTHYTDTRGMRELRDAIRAFYREEFDFEVADEEVAVTTSACQGM
                     WAVMQAVLDEGDEVIVPEPYFSPYKDQIEMAGGVLVPLPTCEEENFEVNVHRIEPLIT
                     PRTKAILFNTPNNPTGCCFDRAALEVIADVAERHDLLVIADDIYTIFSYAGPFIPIGT
                     LPGMAQRTVTVRSFSKDYCMTGWRIGYVIAPPQIIEALQLVGESNVYSAPSISQRAGI
                     AAFRLRREVQPAIVEEFRRRTEAACAAIGEFDYLSVLPPQGSIYLFVNIKRTGLTSHE
                     FAAQLLERYHILVVPGNAFGTSGEGYVRLALTLGVDRIQEAFVRLPKDRF"
     assembly_gap    2083..2092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2678..3418)
                     /locus_tag="ACZPFK_00635"
     CDS             complement(2678..3418)
                     /locus_tag="ACZPFK_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404843.1"
                     /translation="MDNERFGAFVAERRRALGLTQKELARRLHLTDKAVSKWERGLGF
                     PDIAVLEPLAGELEVAVTELLQGRRQQQADAGRAELQTVVADALRLSQEEILLRRRRH
                     RRALWATAALGLLLCFFSAFAVWRLCAAPYPEPFDLLPVPCAWSEGDTGQWREKLPDN
                     SAYDLGVNSEGLPVFQRPFAALALAGAECGDAVAYMKEVYGLPPLTRWTWRGYKNYGW
                     QLGEAEERVQKQGRLLSGILDLYENSFE"
     gene            complement(4066..4560)
                     /locus_tag="ACZPFK_00640"
     CDS             complement(4066..4560)
                     /locus_tag="ACZPFK_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404844.1"
                     /translation="MGAVLGLSFVLALLGTVACAFARGRGASWIALLASGVFGVAGAA
                     LFAWSRAPWRAALASVGPAQATVAAAFARGCGVAFGTVLALSVLVCLFARQRWLQLAA
                     TALTAVVQAAFLPLASLVALSVSPKLEASPLGGLVTAQALSLLLLLYWAHFFSALLRL
                     LRGR"
     gene            complement(5116..5625)
                     /locus_tag="ACZPFK_00645"
     CDS             complement(5116..5625)
                     /locus_tag="ACZPFK_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR1404845.1"
                     /translation="MSIVKNEAKSKNPVIVAVREQLEHRAAWLYLLCDEAQRHGLSWE
                     DFAGDAVRRCGLFQGKGLTGKAGTQSLTGLKKTLFTLPARMVFEMKVLRCDDDHLDLD
                     FGYCPLVKAWQKQGCTDEEIARLCDIAMCGDRAIAESFGCRLELPQTIADGADRCALR
                     FVRAEKGEG"
     gene            complement(5639..6505)
                     /locus_tag="ACZPFK_00650"
     CDS             complement(5639..6505)
                     /locus_tag="ACZPFK_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR1404846.1"
                     /translation="MPCQRDVIYVYDGGLEGMLCCIFEAFEKKERPAAIRPQQELQPS
                     LFETRWVETDAARARRVVRGLEQKVSPRAAWVAQGLFLTAHPEKELLALDFTRLAFQR
                     GPQVTAMMGHPVVSDAVLTVRRAFGEAHQYKGLLRFSDVGGVLAAEIEPKNNVLPMIA
                     PHFCARFRNESFLIYDRVHHAALIWQEHRAQMIQVEALALPPPTADELQTRRLWRRFY
                     KTIAIEARTNPRCQMSNMPKRYWNLLTEMQDEEDDRAALAAAAGALRPRAEYGTIEAE
                     NAAGAGGDAPRG"
     gene            complement(6484..7794)
                     /locus_tag="ACZPFK_00655"
     CDS             complement(6484..7794)
                     /locus_tag="ACZPFK_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR1404847.1"
                     /translation="MDVLDKLTILTDAAKYDVACTSSGAGDTAPAGAGMGSTENCGIC
                     HTWSADGRCVSLLKVLMTNVCVFDCKYCVNRRSNDTPRAAFTPRELADLTMSFYRRNY
                     IEGLFLSSGVRRDPDYATEQIIQALELLRGPYHFTGYIHAKAIPGADPLLIGRLGRLA
                     NRLSVNIELPSQRSLSLLAPNKSKQNILGPMAEIRDNITESRQGLVKYKNAPKFAPAG
                     QSTQMIVGATPETDYQILRLTEGLYHKYSLKRVFFSAYIPVAEDTLLPSLDTKPPLLR
                     EHRLYQADWLLRYYQFSSEEILDEEHQDFNPFLDPKCNWAINHMELFPVEVNRAPYEM
                     LLRVPGIGPVSARRIARARRAGKLDWDDLKRIGVVLKRAQYFLLCKGKGLPGLTVTRQ
                     NTVKALIDGAPVAEAEQLSLFGAEERPRLAEVKEVAAECLASAM"
     gene            complement(8102..8248)
                     /locus_tag="ACZPFK_00660"
     CDS             complement(8102..8248)
                     /locus_tag="ACZPFK_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404848.1"
                     /translation="MAGWELDYLRQQLGGVLCPRQIQRAVWYREEAQMPIESIAVALA
                     YFYA"
     gene            complement(8265..8513)
                     /locus_tag="ACZPFK_00665"
     CDS             complement(8265..8513)
                     /locus_tag="ACZPFK_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404849.1"
                     /translation="MKRSFLLNMLAILAGLVGVAALGGVSLSVSASLYRLFAAAAAFF
                     ISWQLFAAGLAVPAKRQARLHQGGAARGRARAALHRAA"
     gene            8754..9383
                     /locus_tag="ACZPFK_00670"
     CDS             8754..9383
                     /locus_tag="ACZPFK_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR1404850.1"
                     /translation="MRALFPEVLRKLRRDKKLSQGALARELDITQQAVGKWETGRSQP
                     DPEMLRRLADFFSVSADFLLGQAPHGNASPVPAYSAAGEHPIPVVGTVRAGFGALALE
                     EDCGVEYARVKDPENYFYLIVRGRSMEPRIQDGDLALVHRQETLEDGDLGVMVFGDDG
                     EGTLKKFIRRGNAVVLQPFNPDYQPQVLMGEELEHLYIAGKVIETKAKW"
BASE COUNT         1759 a         3385 c         3017 g         1694 t
ORIGIN      
        1 ctctcgcgct cggcggggtt cagctccggc gtcaaggtgc gcagcactcc ggcggccccg
       61 atcacccgcg gcaggctggc ataaacgccc cgctcgccgt attcgccgtt cagcatggcg
      121 ctacagggga tcacccggcg ctcgtcccgc aggatggccg cgcagagggc cgccgccacc
      181 gagccgatgc cgaactcggt gctgcccttg ggggccgcga tctcccaacc ggccgagatg
      241 gtgcagcggg ccgcctcggc cagcgccgcc tcggagcaga gctcctgcac cgggcggccg
      301 ccgatgaccg ccgacgacag cgcggcgacc tggttgtccc cgtgctcgcc cagacagtac
      361 gcctccaccg aagcgggcgc cacgccggcc gcctgcgcga tgcgcaggcg cagccgggcc
      421 gaatccagcg ccgtgccggt gccgatgacg gtgaggcccg tcccggcggc caggtactgg
      481 gcgatgatgt cgcaggggtt ggtgatcgaa accacgacgc cgcaaaagcc gctgcccttg
      541 aggcgcggca ggatttgctg cgccacccgc accgtattgt ccagctccag cagccggtcc
      601 tcctctaaaa tgccgtcgca ggccgccatg accaccaggg cggcgtccgc caggtcgtca
      661 taacccccgg cggcaattgc caccggccgc gggaagcaga ccgccgcgtc cgccaggtcg
      721 atggcctggg cccgcgcctt gtccgcgtcg cagtccacca gcacgatctc atcgcacagg
      781 ccgcccagcg acaaggcgct ggccacatgg ctgcccacat gcccggcccc gatgacgccg
      841 atcttttctt ttttcatggc cggccctctc aaaagcggtc tttgggcagg cggacaaagg
      901 cctcctggat gcggtccacg cccaaggtga gggccaggcg cacatatccc tcgccgctgg
      961 tgccgaaagc gttgcccggc accaccagga tgtgatagcg ctccagcagc tgggcggcga
     1021 actcgtggct ggtcaggccg gtgcgcttga tgttgacgaa cagataaatg ctgccctggg
     1081 gcggcagcac cgacagatag tcgaactcgc cgatggcggc gcaggccgcc tcggtgcgac
     1141 ggcggaactc ctccacgatg gcgggctgca cctcacgccg cagccggaaa gccgcgatgc
     1201 ccgcccgctg cgaaatgctg ggggccgagt agacgttgct ctcccccacc agctgcaggg
     1261 cctcgatgat ttgaggcggg gcgatgacgt agccgatgcg ccagccggtc atgcagtagt
     1321 ccttgctgaa agagcgcacg gtgacggtgc gctgcgccat gccgggcagg gtgccgatgg
     1381 ggataaaggg ccccgcatag ctgaaaatgg tgtagatgtc gtcggcgatg accagcagat
     1441 catggcgctc cgccacgtcg gcgataacct ccagcgccgc ccggtcaaaa cagcagccgg
     1501 tggggttgtt gggggtgttg aagaggatgg ccttggtgcg cggcgtgatc agcggctcga
     1561 tgcggtggac gttgacctca aaattctcct cctcgcaggt gggcagcggc accagcacgc
     1621 cgcccgccat ctcgatctgg tccttatagg ggctgaaata gggctccggc acgatgacct
     1681 cgtccccctc gtccagcacg gcctgcatca cggcccacat gccctggcag gcgctggtgg
     1741 tgacggccac ctcctcgtcc gccacctcaa aatcgaactc ctcccggtaa aaggcgcgga
     1801 tggcgtcccg cagctcccgc atgccgcggg tatccgtata gtgggtatag cccgcctttg
     1861 cgtccgcaaa ggccgcctcg atgatgcggt cgtcggtctt cagatcgggg tcgccgatgc
     1921 tgaaattgat caggtcctca taggccgccg ccttggggcc cagatcccca agcatggtgg
     1981 catggccgtt ctcgtatttt ttcgacacaa actttttttg catgctccgc ctctctcccg
     2041 ccgcgcaaaa cgcgcgcagc cgcttcattt ttctattttt ctnnnnnnnn nncatcataa
     2101 cggccgccgt gcccgctgtc aatgaaacat ctcacaaaaa agccccctca aaaagggggc
     2161 ttttctacgt ctgtgtataa aagcaataaa gcacggggca cattgccccc cggttgcccg
     2221 cggcccgtgg ggatcgcttt cgactttgcc cgccgccgaa aaaacaaggt gcggccgcgg
     2281 tcttcgcggg cttgggcgtc tttgcaggcc cgggcagccg ccggacggcg cttgcccccg
     2341 ttttgccgcc gggccaaatg tcaacccatt cccaggcgac agtgctgagc ggacggtgtg
     2401 ggccgcctgg gcgggcagga cctgcccttg cgcgccggcc cccgccgcgc ccgcgcagag
     2461 gcggcggatc cgggccggga gaaaagccgc cgctttccgc cccgctttct ccgggggcgc
     2521 gggccgggcg agagatcgaa cggcgcaggc cgccggctcg cgtcccgcgc cgttttgctt
     2581 ttgccatccc gccctcaagc cggccagtca gcgcctgact gcggcgcacc gtaaaagcag
     2641 cctccgcgcc tcggcatgct cggccgccga ggcgccctca ttcaaagctg ttctcgtaca
     2701 ggtccaaaat cccgctgagc aggcgtccct gtttttgaac ccgctcttcc gcctccccca
     2761 gctgccagcc gtaatttttg tagccgcgcc aggtccaccg ggtcagcggc ggcaggccat
     2821 agacctcttt catgtaggcc accgcgtcgc cgcactctgc accggccaga gccagggccg
     2881 cgaacggccg ctgaaagacc ggcaggccct cgctgttgac gcccagatca taggcgctgt
     2941 tgtccggcag cttctccctc cactgcccgg tgtccccctc cgaccaggcg caggggaccg
     3001 gcagcaggtc aaagggctcc ggataggggg cggcgcagag ccgccagacc gcaaaggcgg
     3061 agaaaaaaca gagcagcagc cccagcgcgg ccgtggccca gagggcgcgc cgatgacggc
     3121 ggcggcgcaa aaggatctcc tcctgcgaga ggcgcagcgc gtcggctacc acagtctgca
     3181 gctccgcccg cccggcgtcc gcctgctgct gccggcggcc ctgcagcagc tcggtcaccg
     3241 ccacctccag ctcgccggcc agaggctcca gcaccgcgat atcggggaag cccagccccc
     3301 tctcccactt gctcaccgcc ttgtcggtga gatgcagccg ccgggccagc tctttttggg
     3361 tcagccccag ggccctgcgc ctttccgcga cgaaagcgcc gaatctctcg ttgtccatcg
     3421 cccacgcctc ccttgcctct cagcatagcg cgccgggcgc aaaaagacaa tcgactgccg
     3481 gtagagccgc tgtttcccgc ccgcgcgccc ttggcgccgc aggcggctct cggcggcttc
     3541 tggtcgtcgc ggcccgcaag cgcggggcaa aaatgcggac gacgcgtcgc cctggcaccg
     3601 gcgcatccca tccgttttat aaaaagtcat agccatcagt taaaccggcg gtaggcgcag
     3661 attctgcaag gcgggcttac cggcggccct cccaaggcgc gcggaaaggt ctttcccctg
     3721 catcgcctgc cctgtcgccc gagcggggat attcggccgc cagccaagac ttccgccttt
     3781 gcatcctctc tccccgcagc gtcaccgaca gctttcgccc aatggggggc tgttgacaaa
     3841 gccgctcctt gaaagcatgg acgctttctt cccgcatatt tcacaaaaat actcgttaag
     3901 ccgcaaaggc ttgtccgcgt tttttgtttc atccgccgaa aaaaatcgcc tgcgttttct
     3961 gcggtctctg gtttgtcaac cggcccaatg cgggaagcgg aggctttttc ggaagcgccc
     4021 ctttgcagcg gtgaagactt ttaaggggga taaaaacgcg ctgcttcacc gcccccgcag
     4081 cagacgcagc aaagcggaga aaaaatgtgc ccaatacagc aaaagtagca gtgagagcgc
     4141 ttgcgccgtc accagaccgc ccagcggcga cgcctccagc ttaggagaca cgctcagcgc
     4201 gaccagagac gcgagcggca ggaaggccgc ctgcacgacg gctgtcagag ccgtggccgc
     4261 cagctgcaac caccgctgcc gtgcaaacag acagaccagc acggagaggg ccagcactgt
     4321 gccgaaagcc accccgcaac cgcgggcaaa agcggccgcg acagtggcct gcgccggccc
     4381 aaccgaagcc agagccgccc gccagggggc ccggctccag gcaaaaagcg ccgcccctgc
     4441 cacgccgaaa acgcccgagg cgagcaaagc gatccagctc gccccccgcc cacgcgcaaa
     4501 ggcgcaggcc acggtgccca acagagccag gacaaaagag agacccagaa cagcccccat
     4561 ctgcgttttc ctcctcgctt tctttcctag gaacaaaaag ccttttctga ttttaaagcg
     4621 caaagcgcgc agccaggctt ttcttcgcag cgccctcccg gacagtcccc gcgggccgca
     4681 gaaaaaacgt ctcaataatt tggaggctat ttctcctgtc cgctctctcc ggcgctgctt
     4741 cgccgcgtca tcgtcccttt caggggaggc gggttgcccg cgccttgcag ctttcacggg
     4801 acgggaggcg cgcgctcttc ccgcagaagc ttcttttcga gttgcttttt tcacccagcg
     4861 caaggctctc ctctcagggg gactgtcacg caggtgaggg aggggtttgc cgtgccggtt
     4921 cctttgcatc cgaaagacag caacgcttgg ttcaccaacc tggcgtcttt gttccccgcg
     4981 actgcttttc tgccgacggc cccgtttctc gccgctgcgc gggaactgga acagatggcc
     5041 gcaaaaaagg cttatttcaa tggccgtggc ccgaggacag gccacatggc cccaaaaata
     5101 gcctacggct attttttacc cctctccctt ttcggcgcgc acaaaacgca gcgcgcagcg
     5161 gtctgcgccg tccgcgatgg tctgcggcag ctccaggcgg cagccgaagc tctccgcgat
     5221 ggccctgtcg ccgcacatcg cgatatcgca aagccgcgca atctcctcat cggtacagcc
     5281 ctgcttctgc caggctttca ccagcggaca gtaaccgaag tccaagtcca aatgatcgtc
     5341 gtcacagcgc agtactttca tctcaaaaac catgcgcgcc ggcagcgtga acagggtctt
     5401 tttcagcccg gtcaggcttt gggtgcccgc cttgccggtc agccctttgc cctgaaacag
     5461 cccacagcgg cgcaccgcgt cgcccgcgaa atcctcccag ctcaggccgt gccgctgcgc
     5521 ctcgtcacag agcaggtaga gccaggcggc ccggtgctcc agctgttcgc gcaccgccac
     5581 gatcaccggg tttttgctct tcgcctcatt cttcacaata ctcacgcgcc catctccttc
     5641 agccgcgcgg cgcatcgccc ccggcgcccg cggcgttttc tgcctctatg gtaccgtatt
     5701 ccgcccgcgg gcgcaaggcc ccggccgccg ccgcaagagc ggcacggtcg tcctcctcat
     5761 cctgcatctc ggtgagcagg ttccagtaac gcttgggcat gttgctcatc tggcagcgcg
     5821 ggttagtgcg cgcctcgatg gcgatggtct tgtaaaaccg ccgccagagc cgacgcgtct
     5881 gcagttcatc cgccgtgggc ggcggcaggg caagcgcctc cacctggatc atctgcgccc
     5941 ggtgctcctg ccagatcagg gcggcgtggt gcacccggtc gtagatcagg aacgactcgt
     6001 tgcggaagcg ggcgcaaaaa tggggggcga tcatgggcag gacgttgttt ttgggttcga
     6061 tctccgccgc cagcaccccg cccacgtcgg aaaagcgcag caatcccttg tactgatggg
     6121 cctccccaaa ggcccgccgc acggtcagca cggcgtcgga gacgaccgga tgccccatca
     6181 tagccgtcac ctgggggccg cgctggaatg ccaggcgggt gaaatccagg gccagcagct
     6241 ccttttccgg atgggcggtc aaaaacaagc cctgcgccac ccaggccgca cggggcgaca
     6301 ccttttgttc cagtccgcgc accacccgtc gggcccgcgc cgcatccgtt tccacccaac
     6361 gggtctcgaa caggctgggc tgcagctcct gctgcggccg aatggcggcc gggcgctctt
     6421 ttttttcaaa ggcctcgaag atgcagcaga gcatcccctc cagccctccg tcgtaaacgt
     6481 agattacatc gcgctggcaa ggcattcggc cgcaacctcc ttcacctcag cgagccgggg
     6541 ccgctcctcc gcgccgaaaa gcgagagctg ttccgcctcg gccaccggcg cgccgtcgat
     6601 cagcgctttc acggtgttct gccgcgtgac ggtcagcccc ggcagtccct tgcccttgca
     6661 gagcaggaaa tactgcgccc gcttgagcac cacaccaatg cgcttcagat cgtcccagtc
     6721 cagcttgccc gcccggcgcg cccgggcgat gcgcctggcg ctcaccggcc cgatgcccgg
     6781 cacccgcagc agcatctcat agggggcgcg gttcacctcc acggggaaca gctccatgtg
     6841 gttgatcgcc cagttgcact tggggtctaa aaaggggttg aaatcctgat gctcctcgtc
     6901 caaaatctcc tcagaggaga actggtaata gcgcagcagc caatccgcct gatagaggcg
     6961 gtgctcgcgc agcagcggcg gcttggtgtc caggctgggc agcagcgtgt cctccgccac
     7021 cgggatatag gcggaaaaaa agacccgctt gaggctgtac ttgtgataca gcccctcggt
     7081 gagccgcagg atctggtaat cggtctccgg cgtggccccc acgatcatct gggtgctctg
     7141 ccccgccggg gcgaatttgg gagcgttttt gtatttcacg agcccctgcc ggctctcggt
     7201 gatgttgtcc cggatctcgg ccatggggcc caggatattc tgtttgcttt tgttgggcgc
     7261 cagcagcgaa aggctgcgct gggacggcag ctcgatgttg acgctgaggc ggttcgccag
     7321 ccggcccagc cggccgatca gcagcgggtc ggcgcccggg atggccttgg cgtggatata
     7381 gccagtaaag tgataagggc cgcgcagcag ctccagcgcc tggatgatct gctcggtggc
     7441 gtaatccggg tcgcgccgca ccccgctgct gagaaacagt ccctcgatgt agttgcggcg
     7501 gtaaaagctc atggtcaggt cggccagctc cctgggcgta aaggcggcgc gcggcgtgtc
     7561 gttgctgcgc cggttgacgc agtatttgca gtcgaacacg cagacgttgg tcatcagcac
     7621 cttgagcagc gagacgcagc gcccgtccgc cgaccaggtg tggcagatgc cgcagttttc
     7681 agtgctgccc atgcccgccc cggcgggcgc ggtgtccccc gcccccgagg aggtgcaggc
     7741 cacatcgtac ttcgccgcgt cggtcaagat cgtcaatttg tcgagcacat ccatgccctc
     7801 gtcccctttc ccgctggaac atcccgcccg ggcgcggtga aaatcggttt cgcgcgccgg
     7861 gcgaaaaggg cgtcggcacc atctccgtcc gcagcgccgc tgcggcctgg cccccggccc
     7921 gcctcacccg gcggcaggtt ctccagctct gccaccctgc ggcaggggca gagcccccac
     7981 cgaaaagcgc ctgtcgcgcc tttgcgcgcc gcaggcagcg cgcggcgcaa aaaaatcccc
     8041 gcagccgggc gcaggcctgc gcttcgctgc ggggacattt tttcggttca aagggcgccg
     8101 gtcaggcgta aaaataagcc agcgccacgg cgatgctctc aatgggcatc tgcgcctctt
     8161 cccggtacca gaccgcccgc tggatctggc gcgggcaaag gacgccgccc agctgctgcc
     8221 ggaggtaatc cagctcccat cccgccatga aagccgcccc cctctcaggc cgcgcggtgc
     8281 agtgcggcac gcgcacgccc ccgcgccgcg cccccctggt gcaggcgggc ctgtcttttg
     8341 gccgggacgg ccagcccggc ggcaaagagc tgccagctga tgaaaaacgc ggccgccgcc
     8401 gcgaacaggc gataaagtga agcgctcacg gacagggaca caccgcccag ggccgcgacg
     8461 ccgaccaggc ccgccaggat agcgagcata ttgagcaaaa acgatctttt cattttactt
     8521 ccccctctgt cgggagcccg cctgcgacgt gcccccgcgc gccgccatcc ggcgcaggtc
     8581 ccgcgcgggc accaccccgc tgcggcacga cttttctgtg gttccatgat aatacaaccg
     8641 gcagttgttg tcaagcgtta taacaactaa aatttgtttt aacaaccata ttttattttt
     8701 ctactttaca accacaacta ttagttgtat actaacctta ttaccacaag gaggtgcgcg
     8761 cgttgttccc cgaggtgttg cgcaaattgc gccgtgataa aaaattgagc cagggcgccc
     8821 ttgcccggga gcttgacatc acccagcagg ccgtgggcaa atgggagacg ggccgcagcc
     8881 aaccggaccc ggagatgctg cgccgtctgg ccgacttctt cagcgtgagc gcggattttt
     8941 tgctggggca ggcgccgcac gggaacgcct ccccagtgcc ggcttacagc gcggcgggcg
     9001 agcaccccat cccggtcgtg ggcaccgtgc gggccggttt cggcgccctg gcgctggagg
     9061 aggactgcgg cgtcgagtac gcccgggtga aagaccctga aaactatttc tatctgatcg
     9121 tgcgcggccg cagcatggag ccccgcatcc aggacggcga cctggcgttg gtacaccggc
     9181 aggaaacgct ggaggacggc gacctgggcg tgatggtctt cggcgacgac ggcgagggca
     9241 ccctgaaaaa attcatccgc aggggcaatg ccgtcgtgct gcagcctttc aacccggact
     9301 atcagcccca ggtgctgatg ggtgaagagc tggagcatct gtacatcgca ggcaaggtca
     9361 tcgagacaaa agccaagtgg tgaacagggg agcgcaggga ccggacataa aaccgaacta
     9421 aaaaagcatg cggccgcgcc cagcggccgc atgctttttt actgtactgc caaagcaact
     9481 tgttttggcg atctcggggc aaaggaagct cacccggcct aaaaatcatt gggggctgtg
     9541 gacaaaccaa ggggcgcaga aaacgcagga aagcctttct ggctcaacga gtatttgtgt
     9601 ggaatatgcc acagtaaagc gcccatgttt tcaaggaaag cttttgccaa aagtcctaat
     9661 ttgtcttaat agctccgttc gggcagggct tttggcgacc gaccgcaggg gacccgccgc
     9721 tcaggacgca aaaaagtgcg gtgcccacag gcgccgcact gaaaatatcc cgcgcgtttc
     9781 gttctcctcc cctattgcca ctccagcacc gtgccggcgc cgcagggggc gtaggcgcgg
     9841 cagtggtcgg caaagagctg cagcg
//
LOCUS       DBMDYA010000012         9780 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1907_length_9780_cov_1.658642, whole genome shotgun sequence.
ACCESSION   DBMDYA010000012 DBMDYA010000000
VERSION     DBMDYA010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_9780_cov_1.658642"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            636..3146
                     /locus_tag="ACZPFK_00675"
     CDS             636..3146
                     /locus_tag="ACZPFK_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1404851.1"
                     /translation="MEHSLQNKPRWRRPALLAFGISALAALALFLPFLIIDKGFFLYC
                     GDFNSQQIPFYQYCQQFVKSGGGAFSWATDLGSGFLNTYSFYNLGSPFFWLSCLLPNR
                     WLPYAMVPLLCLKFGVGGLGAYLYMRRYTKSANFALVGSALFTLSGWGVYNIFFNHFI
                     DCMVLFPFLLAALDSYIYDKQRAVLPLAIALNLLNNYFFFLGEALFLALYFFIKLGAG
                     DYRLTVREFFRLAFEVVVGCLLGCLLIFPAAASLLNNPRTDSYANGYGMLLYGRVQQY
                     FAILRSHFIPPDPPYLPGIYSEATVKWTSMSGYLPLAGMAGVLAWCRGRRGSPHRRVL
                     AVCLVMALVPILNSAFYAFNSSYYARWYYMPVLLMALATANALEDADVDLVGGIKLAA
                     LLTAAFAVFGLVPAKEDDRWKLGVADNAAQFWLTLLLALLGLLIFYCIACWHRGRRRF
                     APLMLAAVLGFGVLNGVVHLSIGKFPQWDNDANYKLQNYDVVEQIELPGDRFYRIDTY
                     NCYDNLGLFLDRPCIRCFNSTVTPSILQFYPGVGVKRDVSSKPELELYALRGLLSVEY
                     LLMPAGSQADFLAEDAQTGFVFDGLTGDGVYARYRNENAVPMGFTYDYYVLPETVETV
                     SKEKRGNLYLRAIGLSEEQAAQYGQNMEPLPEALAGGFSYESYVQDCADRRARACSSF
                     AAMRDGFTAAITLEKDNLVFFSVPYDEGFAATVNGEAAEVLNVDGGLIAIPAKAGENE
                     ITLTLTPKWLPLSCGLTAAGALLYAGYLALTLLRHHRQGPLPGRFVPLEVAAGHPSAL
                     WDGAEESEQLLLMEDEARGGEDRPAQAPPPPEPPRESE"
     gene            3152..5317
                     /locus_tag="ACZPFK_00680"
     CDS             3152..5317
                     /locus_tag="ACZPFK_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIR1404852.1"
                     /translation="MDFVKTLAGEFETRPDYVQNIIALIDEGNTIPFIARYRKEQTGS
                     MDDQVLRRLDERLTYLRGLERRKAEVASSIEEQGKLTRELQAALDAAETLAQVEDLYR
                     PYKPKRKTRASVARERGLEPLAQLIFAQQDGPPALTAAEAYVDAERGVESAEAALAGA
                     LDIIAEDLSDDARLRGLLRGFTFHTGIVSSKAAVKEDSVYAQYYDFREPASKIAGHRV
                     LALDRGEREKFLKVSVEVDVEQAVGLIAASAVKGKGEYSALVARAAADAYARLIAPSL
                     ERELRAALTEQAAEGAIKVFSDNLRQLLLQPPIKGKVALGMDPGYRMGCKLAVVDATG
                     KVLTTAVIYPVPPHSRVEQAKATVKQLIRTYGVEIIAIGNGTAGKETEMFAAEVLREL
                     GGGASYMVVSESGASVYSASPLAAEEFPDFDVNVRSAVSIARRLQDPLAELVKIDPKA
                     VGVGQYQHDMPQAKLDEALAGVVEACVNTVGVDLNTASAPLLSHVAGITKTTAKNIVA
                     RRETEGPYQSRRELLKVPKLGPKAFEQCAGFLRLPGAKEPLDATAVHPESYAAAKGLL
                     ARCGFAEADIGTPALDALEERMRAYGRKRLAEELGVGLPTLADIAAELRRPGRDMRDG
                     LPKPLLRTDVMSMEDLKPGMELTGTVRNVIDFGAFVDIGVHQDGLVHISQISNRYIKH
                     PSEVLKVGDVVTVWVLSVDTAKKRIGLTMKKPREAQKNP"
     gene            complement(5500..6615)
                     /locus_tag="ACZPFK_00685"
     CDS             complement(5500..6615)
                     /locus_tag="ACZPFK_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404853.1"
                     /translation="MASISLRNIYKIYPGDVTAVKDFNLEIEDKEFIILVGPSGCGKS
                     TTLRMIAGLEEISKGELYIGDRLVNDVAPKDRDIAMVFQNYALYPHMTVYKNMAFGLE
                     LRKTPKEEIDRRVHEAAKVLDIEHLLDRKPKALSGGQRQRVALGRAMVRNPAVFLLDE
                     PLSNLDAKLRTAMRTEIIKLHKRLGTTFVYVTHDQTEAMTMGDRIVVMKDGVVQQVNT
                     PQNLYDFPCNMFVAGFIGSPQMNFLDATLIQENGGFVCDFGDQKMPVPAAKAEANPGL
                     EQYVGKAVKVGVRPEDIKDDPEFVAAHPQETFEADVDVSELMGSEVYLYLDCRGAKMT
                     ARVAPATASRTGSHVKVAFDTAKLHLFDAESELAILN"
     gene            6789..7571
                     /locus_tag="ACZPFK_00690"
     CDS             6789..7571
                     /locus_tag="ACZPFK_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404854.1"
                     /translation="MIFDANALCRAGELVDHRGSAPLRLAGGLWLLCLSRGLCGAQGG
                     RGGPLLLEPGCLLLTSEALVLCPTLPCHLAGVRFAGALADQVADALAGTVAEDGGRCP
                     GAADALALLASGRAAPSPAFCFDLLCRAARLDEAPARVAPLAAQAMAAIRKNYASLYG
                     VDELAAALGVSKGHLVRSFKAATGVTPGRYLTSVRVEAAKQLLRSTDAPLELVASLAG
                     FSGANYLCRVFKAETGRTPAAWRAAPGPEDGAPALPMDEIYV"
     gene            7681..8730
                     /locus_tag="ACZPFK_00695"
     CDS             7681..8730
                     /locus_tag="ACZPFK_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1404855.1"
                     /translation="MPSLEPALTVLCAPALSLLLFALCWAARPGLLGGFFANLTILLG
                     GAAFAWFALSSRNVAAQALLALLGILVVLTALFGLYLLIVLLLLNSRVMLQRERPRLA
                     NMLTLLLALGLIALVALAAFDPLRWLPRPLRSFLGGCSLMLPYLLFDAMNFLTAAGLC
                     ALHRPLGRLDYILVLGSGLIDGDRVPPLLARRIERGIRLYEKQKRRGHAPKLLFSGGQ
                     GADETVAEAEAMRRYALAAGVPEADLLVEDNSRSTAENLKFSKKIMELRSEGRFRCVF
                     VSNGYHLLRASMIARSQGLRARGVGARTAAYYLPNALLREYVAVSAMEKRRHLVVCGL
                     IALFSALLAAAWALK"
     gene            complement(8896..9123)
                     /locus_tag="ACZPFK_00700"
     CDS             complement(8896..9123)
                     /locus_tag="ACZPFK_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404856.1"
                     /translation="MRRYRLRELREDHDLSQQALADYLKITQTSYSKYGLGQRGIPVE
                     TLDQLADYYHTSVDYLIRRTDKKQPYPKSEG"
     gene            9215..9490
                     /locus_tag="ACZPFK_00705"
     CDS             9215..9490
                     /locus_tag="ACZPFK_00705"
                     /inference="COORDINATES: protein motif:HMM:NF044580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HIR1404857.1"
                     /translation="MKMVNGLADLYDRAYFPNQPEPSDAEYADAKQERARCEKKIAAL
                     LPKESQSLPREYLHRTRLLMTMQACHHFCIGWSAAARQLFAGLGGDI"
BASE COUNT         1583 a         3265 c         3122 g         1810 t
ORIGIN      
        1 catccgcgag agcagcgcgg aaaacggata aacagtcatt ggacatcctc ttttcttctc
       61 ttgatctttt gcctcccggc agggcggcgc ggccacgtcc gaatcggccc cgcggcagaa
      121 aaatgtgaaa aatgcctgcg gcgcgtctgt tttgcggccc gaaactcgtt actgaggtca
      181 gaggcctttt gaaaagcgca ggagcggcgg ggcgcgtctg gcccggcagg ccaaagccgc
      241 gcggcggccg tgggcggaag aggtccgatc tccgggcctg cgggcggccc tgacgcggcg
      301 gacaggcctt ttccgccaaa atcaggcggc ctgcgtggcc gcccggggct gcgccggggc
      361 gcggcgcgcc ctgtcctttc cgtttttgaa gccgcgtcac gccgcatgcc ggggcagcgt
      421 ctttttcgcc cggcccgcag cggcggtccg ccacggcggt ccgccacggc cggacgcctg
      481 gcgcagaccg ccgctgcggt gtccaaacgc ccgcggccgc cacagccccg gcgtcccggc
      541 gctttttctc ttttcaatag gtcttggaac tattatagat ggcccgcgcc cgctgcgcaa
      601 gcgccgtgcg gccacatctc tctggagggg atcacttgga acacagcctg caaaacaaac
      661 cgcgctggag gcgcccggcg ctgctggcgt tcggcatttc ggcgctggcc gcgctggcgc
      721 tttttctgcc gtttctcatc atcgacaagg gcttcttttt gtactgcggc gacttcaaca
      781 gccagcagat cccgttttat cagtattgcc agcagttcgt gaaatccggc ggcggggcgt
      841 tcagctgggc caccgacctt ggcagcggtt ttttgaacac ctactctttc tacaacctgg
      901 gctcgccgtt tttttggctg tcctgtctgc tgccgaaccg ttggctgccc tacgccatgg
      961 tgccgctgct ctgcctcaag ttcggggtgg ggggcctggg cgcttacctc tatatgcggc
     1021 gctacaccaa gtcggccaac ttcgcgctgg tgggcagcgc gctgttcacc ctttccggct
     1081 ggggcgtcta caacatcttt ttcaaccatt tcattgactg catggtgctg ttcccgtttt
     1141 tgctggcggc gctggacagc tatatctacg acaagcagcg ggcggtgctg ccgctggcta
     1201 tcgccctcaa cctgctcaac aactacttct tttttctcgg cgaggcgctg tttttggcgc
     1261 tctacttttt catcaagctg ggcgccgggg attaccggct cacggtgcgg gaattcttcc
     1321 gcctcgcctt tgaggtggtg gtgggctgcc tgctgggctg cctgctgatc ttccccgccg
     1381 cggccagcct gctgaacaac ccgcgcaccg acagctacgc caacggttac ggcatgctgc
     1441 tctacggccg ggtgcagcag tatttcgcca tcctgcgcag ccatttcata ccccctgacc
     1501 cgccctacct gcccggcatc tacagcgagg ccacggtcaa atggaccagc atgtccggct
     1561 atctgcccct ggccggcatg gccggggtgc tggcctggtg ccgcggccgt cgcggctcgc
     1621 cccacaggcg cgtcctcgcc gtctgcctgg tgatggcgct ggtgcccatc ctcaactcgg
     1681 ccttttacgc gttcaactcc agctattacg cccgctggta ctatatgccg gtgctgctga
     1741 tggcgctcgc caccgccaac gccctggagg acgccgacgt ggaccttgtg ggcggcatca
     1801 agctggcggc gctgctcacc gcggcctttg ccgtattcgg gctggtgccc gccaaggagg
     1861 acgaccgctg gaagctgggc gtggccgaca acgccgccca gttctggctg accctgctgc
     1921 tggccctgct ggggctgctg atcttttact gcatcgcctg ctggcaccgg ggccgccgcc
     1981 gctttgcgcc gctgatgctg gccgcggtgc tgggctttgg ggtgctgaac ggcgtcgtcc
     2041 atctctccat cggcaagttc ccccagtggg acaacgacgc caattacaag ctgcaaaact
     2101 atgacgtcgt ggagcagatc gagctgccgg gcgaccgctt ctaccgcatc gacacctaca
     2161 actgctacga caatctgggg ctgtttctcg accgcccctg catccgctgc ttcaacagca
     2221 ccgtcacccc cagcatcctg cagttctacc ccggcgtggg ggtcaagcgg gacgtctcct
     2281 ccaagccgga gctggagctg tacgccctgc gcgggctgct gagcgtggag tatctgctga
     2341 tgcccgcggg cagccaggcg gattttctgg ccgaggacgc ccagaccggc ttcgttttcg
     2401 acggcctcac cggcgacggc gtctatgccc gctaccgcaa cgaaaacgcc gtgcccatgg
     2461 gcttcaccta cgactactat gtgctgcccg aaacggtgga gacggtgtcc aaggaaaagc
     2521 gcggcaacct ctacctgcgg gccatcggcc tcagtgagga gcaggccgcg cagtatggcc
     2581 agaacatgga gcccctgccc gaggccctcg ccggcggctt ctcgtatgag agctacgtgc
     2641 aggactgcgc cgaccggcgc gcccgtgctt gcagcagctt tgcggccatg cgggacggct
     2701 tcaccgccgc catcacgctg gaaaaggaca atctggtctt tttctcggtg ccctatgacg
     2761 agggctttgc cgccacggtc aacggcgagg ccgccgaggt gctgaacgtg gacggcgggc
     2821 tgatcgccat ccctgccaag gcgggagaaa acgagatcac cctgaccctg acgcccaagt
     2881 ggctgcccct ctcctgcggc ctcaccgccg ccggcgcgct gctgtatgcg ggctatctgg
     2941 ccctcaccct gctgcgccac caccggcagg gccccctgcc cggccgcttt gtgccgctgg
     3001 aggtggccgc cggccatccc agcgccctct gggacggcgc ggaggagagc gaacagctgc
     3061 tgctgatgga ggacgaggcc cggggcggag aagaccgccc cgcacaggcc ccgccgcccc
     3121 cggaaccccc aagagaaagc gagtgacccc catggatttt gtcaaaacgc tggcgggcga
     3181 atttgagacc cgcccggact atgtgcagaa catcatcgcc ctcatcgacg agggcaacac
     3241 cattcccttt atcgcccgct accgcaagga gcagaccggc agcatggacg accaggtgct
     3301 gcgccgcctg gacgagcgcc tgacctatct gcgcgggctc gagcgccgca aggccgaggt
     3361 cgcgtcctcc attgaggagc agggcaagct gacgcgcgag ctgcaggccg cgctggacgc
     3421 cgccgagacg ctggcccagg tggaggacct ctaccgcccc tacaagccca agcgcaaaac
     3481 ccgggccagc gtggcgcggg agcgcggcct tgagccgctg gcgcaactga tcttcgccca
     3541 gcaggacggc ccgcccgccc tcacagccgc agaggcctat gtggacgcgg agaggggggt
     3601 ggagagcgcc gaggccgcgt tggcgggcgc actggacatc atcgccgagg atctgagcga
     3661 cgacgcccgc ctgcgcggcc tgctgcgcgg cttcaccttt cacaccggga tagtgtcgag
     3721 caaggccgcg gtgaaagagg acagcgtcta tgcccagtac tacgattttc gcgagcccgc
     3781 gtccaagatc gccggccacc gggtgctggc gctggaccgg ggcgagcggg aaaagttttt
     3841 gaaagtctcg gtggaggtgg acgtcgagca ggccgtgggc ctgatcgccg cctcggccgt
     3901 gaaaggcaag ggcgagtaca gcgccctggt cgcccgggcg gcggcggacg cctacgcgcg
     3961 gctcatcgcc ccctcgctgg agcgggagct gcgcgccgcc ctcaccgagc aggccgccga
     4021 gggcgctatc aaggtgttca gcgacaacct gcgccagctg ctgctgcagc cgcccatcaa
     4081 gggcaaggtg gccctaggca tggaccccgg ctaccgcatg ggctgcaagc tggcggtggt
     4141 ggacgccacc ggcaaggtgc tgaccacggc cgtcatctat ccggtgccgc cccacagcag
     4201 ggtggagcag gccaaggcca ccgtcaaaca gctaatccgc acctacgggg tggagatcat
     4261 cgccatcggc aacggcaccg ccggcaagga gaccgagatg tttgccgccg aggtgctgcg
     4321 cgagctgggc ggcggcgcct cctacatggt ggtcagcgag agcggcgcct cggtctactc
     4381 cgcctccccc ctggccgccg aggagttccc cgatttcgac gtgaacgtgc gcagcgccgt
     4441 gagcatcgcc cgccgcctgc aggacccgct ggccgagctg gtgaagatcg accccaaggc
     4501 ggtgggggtg ggccagtacc agcacgatat gccgcaggca aagctggacg aggccctggc
     4561 gggcgtggtg gaagcctgcg tgaacacggt gggggtggac ctgaacaccg ccagcgcccc
     4621 gctgctctcc catgtggcgg gcatcaccaa aaccaccgca aagaacatcg tggcccgccg
     4681 cgagaccgag ggcccctacc agagccgccg cgagctgctc aaggtgccca agctgggccc
     4741 caaggccttt gagcagtgcg cgggctttct gcggctgccc ggcgcaaagg agccgctgga
     4801 cgccaccgcc gtgcaccccg agagctacgc cgccgccaag gggctgctgg cccgctgcgg
     4861 ctttgccgag gcggacatcg gcaccccggc gctggacgcg ctggaggagc ggatgcgcgc
     4921 ctacggccgc aagcgcctgg ccgaggagct gggcgtgggc ctgcccactc tggccgacat
     4981 cgccgccgag ctgcgccgcc cgggccgcga catgcgcgac gggctgccca agccgctgct
     5041 gcgcaccgac gtgatgagca tggaggacct gaagcccggc atggagctca ccggcacggt
     5101 gcgcaacgtc atcgatttcg gcgctttcgt cgacatcggc gtgcaccagg acgggctggt
     5161 acatatcagc cagatctcca accgctacat caagcacccc agcgaggtgt tgaaagtggg
     5221 cgacgtggtg acggtctggg tgctgagcgt ggacaccgcc aaaaagcgca tcggcctgac
     5281 catgaaaaaa ccgcgggaag cgcagaaaaa cccgtgatcg aacagagaga ggggccgcac
     5341 ccgatgggtg cggcccctcc tgctttctct taaaaaacgt ctgcccgtcg gtttgttttt
     5401 tcgcgggccg gaatgaaatt ttttcccgct ctccagggag tgagcgcaaa agggacgcgg
     5461 acttttcgtc cgcgccccgc cggggaagcc ccgccttgtt cagttcagga tggcaagctc
     5521 gctctcggcg tcaaagagat gcagctttgc ggtgtcgaaa gcgactttca cgtggctgcc
     5581 ggtgcggctc gccgtggccg gggccacacg ggccgtcatc ttggcgccgc ggcagtcgag
     5641 gtacaggtag acctcgctgc ccatcagctc ggagacgtcc acgtccgcct caaaggtctc
     5701 ctgcgggtgg gcggcgacaa actccgggtc gtccttgatg tcctcggggc ggacgcccac
     5761 tttcacggcc ttgcccacat actgctccag gccggggttc gcctccgcct tggcggcggg
     5821 caccggcatc ttctggtcgc cgaaatcgca gacaaagccg ccgttttcct gtatcagcgt
     5881 cgcgtccagg aaattcatct gcgggctgcc gataaagccc gccacaaaca tgttgcaggg
     5941 gaagtcgtaa aggttctgcg gcgtattgac ctgctgcacc acgccgtcct tcatgaccac
     6001 gatgcggtcg cccatggtca tggcctcggt ctggtcgtgg gtgacataga cgaaagtggt
     6061 gcccagccgc ttgtgcagct tgatgatctc ggtgcgcatg gcggtgcgca gcttggcgtc
     6121 caggttggac agcggctcat ccagcagaaa gaccgccggg ttgcgcacca tggcgcgccc
     6181 cagggccacc cgctgccgct ggccgccgga cagcgccttg ggcttgcggt ccagcaggtg
     6241 ctcgatgtcc agcacctttg ccgcctcgtg gacgcggcgg tcgatctcct ccttgggggt
     6301 cttgcgcagc tccagcccaa aggccatgtt cttgtacacg gtcatatgcg gataaagggc
     6361 gtagttctgg aacaccatcg cgatgtcccg gtccttgggg gccacgtcgt tcaccagcct
     6421 gtcgccgatg tacagctcgc cctttgagat ctcctccagg cccgcgatca tgcgcagcgt
     6481 ggtggatttg ccgcagccgg aggggccgac caggatgatg aactccttgt cctcgatctc
     6541 caggttgaag tccttgacgg cagtgacgtc gccggggtag atcttgtaaa tgtttctcag
     6601 gctgatgctc gccatatggt gttcctccac aatttcgtct gttttcatcc cgccccggcc
     6661 gctgcgtcag cgcggacggc cggaagcttt tcgatagtat taatttgacc tttttatcag
     6721 gttctagtac aataatatca ccaaaaaccc ggaatactat agcaaaatat tgatgtgtgg
     6781 tgtcaaaatt gatctttgat gcaaacgcgc tgtgccgcgc cggcgagctg gtggaccacc
     6841 gcggcagcgc gcccctgcgg ctggccgggg ggctgtggct gctctgcctc tcccgcgggc
     6901 tctgcggggc ccagggcggg cggggcgggc cgctgctgct ggagcccggc tgcctgctgc
     6961 tcaccagcga ggcgctggtg ctctgcccca ccctgccctg ccatctggcg ggggtgcgct
     7021 tcgccggtgc gctggccgat caggtcgcgg acgcgctagc gggcacggtc gctgaggacg
     7081 gcgggcgctg cccgggcgcg gccgacgcgc tggccctgct ggcctcgggc cgggccgccc
     7141 ccagcccggc tttctgcttc gacctgctct gccgcgcggc gcgccttgac gaggccccgg
     7201 cgcgggtggc cccgctggcg gcacaggcta tggccgccat ccggaaaaat tacgccagcc
     7261 tctacggcgt ggacgagctg gccgccgcgc tgggggtgag caaggggcat ctggtgcgca
     7321 gcttcaaggc agccaccggc gtcaccccgg gccgctacct cacctcggtg cgggtggagg
     7381 ccgcaaagca gctgctgcgc tccaccgacg cgccgctgga gctggtggcc agcctggccg
     7441 gtttttccgg cgccaactat ctgtgccgcg tcttcaaggc tgagaccggg cgcaccccgg
     7501 cagcctggcg ggccgccccc ggccccgagg acggcgcgcc cgccctgccc atggacgaaa
     7561 tctacgttta accgcctctt ttcgcgcaaa aagcagtata aagtgctata atggggcaga
     7621 atacgccggg cgcttgcaaa gacgagtccg aatcaggcgc ccggccgagg aggtcctccc
     7681 atgccgtcgc tcgaacccgc tctcaccgtc ctgtgcgccc ctgccctatc tctgctgctg
     7741 ttcgccctct gctgggcggc acggccgggg ctgctgggcg gtttttttgc aaatctcacc
     7801 attctgctgg gcggcgcggc ctttgcctgg ttcgccctct ccagccgcaa tgtggccgcc
     7861 caggccctgc tcgccctgct gggcattttg gtggtgctga cggccctgtt cggcctctat
     7921 ctgctcatcg tcctgctgct gctcaacagc cgggtcatgc tgcagcggga gcggccccgc
     7981 ctggccaaca tgctgaccct gctgctggcg ctgggcctga tcgccctggt ggccctggcc
     8041 gcctttgacc cgctgcgctg gctgccccgg ccgctgcgca gctttttggg cggctgctcg
     8101 ctgatgctgc cctatctgct cttcgacgcg atgaactttc tcaccgcggc cggcctctgc
     8161 gccctgcacc gcccgctggg caggctggat tatatcctgg tgctgggcag cggcctgatc
     8221 gacggcgacc gggtgccgcc gctgctggcc cggcgcatcg agcgcggcat ccgcctttat
     8281 gaaaaacaaa agcgccgcgg acacgcgccg aagcttctct tttcaggcgg ccagggggcg
     8341 gacgagaccg tggcggaggc ggaggccatg cggcgctacg cgctggccgc gggcgtgccg
     8401 gaggcggacc tgctggtgga ggacaactcc cgcagcaccg ccgagaacct gaaattttca
     8461 aaaaagatca tggagctgcg cagcgagggg cgctttcgct gcgtcttcgt ctcaaacggc
     8521 taccatctgc tgcgggcctc gatgatcgcc cgcagccagg ggctgcgcgc ccgcggggtg
     8581 ggcgcgcgca ccgccgctta ttacctgccc aacgccctgc tgcgggagta cgtcgcggtg
     8641 agcgccatgg aaaagcgccg ccatctggtg gtctgcggcc tgatcgcact gttcagcgcc
     8701 ctgctcgcgg cggcgtgggc cctgaaatga gccccggccg cagtgtccgc gcggccctac
     8761 cgccgcaaac cgccccgtta caccccattt ttaaaagcga gggctccgac caaagtcgaa
     8821 gccctcgctt ttaaaagccg ccgtctttta gaggctttga gacccgttcc ccgccgcagc
     8881 cgcagacgcc cctgtctacc cctcactttt gggatagggc tgttttttgt ccgttctgcg
     8941 gatgagataa tccacgctgg tgtggtaata atccgctaac tgatccagtg tctccaccgg
     9001 tatcccgcgc tgccccagtc catactttga ataagaggtc tgcgttattt tcagatagtc
     9061 tgccagagcc tgctggctca aatcatgatc ttcccgcagt tccctcaagc gataccgtct
     9121 catattctca cctcattgtc agtatacatt aagccgtttt ggggtattga tttttaggac
     9181 atttttgggg tatcgcgttt tgggaggtgt ttttatgaag atggtaaacg ggttggccga
     9241 cctctatgat cgcgcctatt ttcccaatca gcctgaaccc tcggatgcgg aatacgcgga
     9301 tgcaaagcag gaaagggccc gctgcgagaa aaaaatcgcc gccttattgc caaaagaaag
     9361 ccaatccctc ccgcgggaat acctccatcg cacgcggctc ctcatgacga tgcaggcctg
     9421 ccatcacttc tgcatcggct ggtcggctgc ggcaagacag ctgtttgccg ggctgggcgg
     9481 cgatatttaa caatttcttc atgtcatccg tttgacaccc cccgccccgc ggtgtaaaat
     9541 gagtaagtgt atgcgcgcgg gccaaaaccg ggcgctgccg ggtttttgca ccccaagaca
     9601 cgacaatttt gtaaaaagat taggcggtaa tcaaatggct tttctcgaaa agattttcgg
     9661 cagcttttcc gacaaagagc tgaaaaagat ccggcccatc gcggaccagg tgctggcgct
     9721 ggagggcaag tataaaggcc tctccgacgc cgagctgcag gcggtgaccc cggccctgaa
//
LOCUS       DBMDYA010000013         9711 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1922_length_9711_cov_1.681686, whole genome shotgun sequence.
ACCESSION   DBMDYA010000013 DBMDYA010000000
VERSION     DBMDYA010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_9711_cov_1.681686"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="ACZPFK_00710"
     CDS             complement(<1..510)
                     /locus_tag="ACZPFK_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404858.1"
                     /translation="MFPDDLCGPKAVIAALRLRGGGRKEKLDRCRREAELYTGCGVSA
                     LLIADRGAELQDLAAALELLRGWPCPLGVSAGGDFFKSWELARRSGAKFIFAASVAGQ
                     LRREEDAPYAEQLAACRAGSQIRVIGGVRFEQQPILSGRPWAEDLRLGMERCDAVAVS
                     GSGGGRETPA"
     gene            complement(1025..2182)
                     /locus_tag="ACZPFK_00715"
     CDS             complement(1025..2182)
                     /locus_tag="ACZPFK_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1404859.1"
                     /translation="MESSKKKLNLLGVLIFLFLGMLNAGTQAVVNNLARAWGATESQL
                     GLMISTFYGGSVFVAVFLGELSERVGKRYGLCAATVSLMAGSLLILMSPHFYMLLAGL
                     LLCGFAFGGMESLTLSLLEDVNDSKARKVFGVTEITLGLGGVLAPILISWGLKSDAYK
                     PMYLILLMVGAALTFSFLRLSSIDSFAKKQEKRPSLSILQTLKNKALRRSMIAVFLFL
                     GVESTVTCWTVTLMANQGFEKIGNIGISAYWLAFMICPYLSTKVKNYFRWFPFCFALG
                     GLGLLALVFAPWGWAKLLAMIFTGIMISPAFANLMFIGGHSVRENSAAGYSMMVFGGN
                     LGSILFQPGAARIVEFFSVEALYLALAMGCLVNGIVIFSCVRAYQAEKLPV"
     gene            complement(2249..3172)
                     /locus_tag="ACZPFK_00720"
     CDS             complement(2249..3172)
                     /locus_tag="ACZPFK_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404860.1"
                     /translation="MHDVLSMVLSEDFLATIIRVGVPLMFASMSAYIAATSGLLNIAI
                     EGIMLMSALMAVLGSYWTQSALLGLLIAVASGILLALLLAFMTMKLGTDPFLVGIAIN
                     TFTATFTIFILYIFSGNKGISASLPSKVLPTVTVSWLEKIPFLRAVFSDQYLLTYVCF
                     AFMILIPLLVGKTSYGLRLKAAGLNPAAAKSVGINVNRMQVSAIVLSGLLAALGGAYM
                     TMGYLSAFSRNMIAGRGWIGFAAQAMGGSSFLGLSVTTLAFSFFQAIVAVLSRTDVSS
                     DLLTTIPYFGVLLGVIAFSVVNYLKKKKLSK"
     gene            complement(3165..4232)
                     /locus_tag="ACZPFK_00725"
     CDS             complement(3165..4232)
                     /locus_tag="ACZPFK_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404861.1"
                     /translation="MAKLKLSDEKVFGVIKTLTALLIALAITFIALCFTAEAPLQSFA
                     TMLTAPLTKIRYFSNVLEMMTPLVFTALACAILFRAGIFNLGVEGIFFICGLVTTYAA
                     TQLALSSHFLHPLLAILLSGTVGGLLMLIPGFLKAKFDTNIVVSTLMLNSIYLGIGLW
                     LLKDKMLATDISVTASPLFAKSAKLPYLFSGTRITVGFFLALLAAVLVYVMMNKTRLG
                     YKIRISGQNANFAKYSGIGAFSLILLIHFISGFLAGVGSSVELLSFYDRFTWTKITTH
                     GATGMLIAMMGNNRVVPVMIAAFGISYLKIGAEIMSRSTVVPVEIISIVEATLVLLIS
                     SQYFLRGLREKKLLKEGMADA"
     gene            complement(4225..5784)
                     /locus_tag="ACZPFK_00730"
     CDS             complement(4225..5784)
                     /locus_tag="ACZPFK_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404862.1"
                     /translation="MKETILELQNITKVYHNGVVANRNISISFKKGEIHSIVGENGAG
                     KSTLMKIIYGIESPSDGQIIHKGEPRKFRGSMEAIQAGIGMVHQHFMLIPGMSVVQNI
                     IVGDEPRKLRSIVDYKSAVRQTKELSEHYHLDVDVLSKVENLSAGEKQKVEILKTLYR
                     NAEIIILDEPTSVLTPQETETLFEELQKLREMGHTIIFISHKLNEVKRISDRITVIRG
                     GEGKGTYDNEEVDLNRLAELIVGRKLLSSYDALKKDVGEHREILRVEGLELHSKTKTL
                     LDNISFSLQSGEILGVAGVQGNGQSELVRALAGIGAMNSGKITLDGRRIEALSVKKRR
                     LLGLSYVPEDRLVDGVAETASLRDNIISTYYDREDINGRFFMDQKKISETAEKLVSKF
                     AIHTSSIHTAVSNLSGGNMQKVVVAREDNTHPVCMLAEQPTQGIDIGSSEYIRTQLIE
                     MRNRGAAVLLISADLDEVISLSDRLIVMYNGTISAYFKDVKSLSLQELGLYMLGVKKQ
                     TPEELEGALCG"
     gene            complement(5855..6931)
                     /locus_tag="ACZPFK_00735"
     CDS             complement(5855..6931)
                     /locus_tag="ACZPFK_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1404863.1"
                     /translation="MKKVLSVFLALILTLSLAACGPEKVAGGEKVWKVAVLLPYIGDQ
                     SYYDAVEAGRKTLDAREEIEATLIEIGTDQAQWESYFMDACESGYDLIVSGYADVEPT
                     LYSAAKMYPDQLFFNFDYSTHEDLDNVFAVTYSLSELGYLAGTLSSLVTTSGMEKANP
                     ENKVGVIVGEDSEYMNDFIGSYCQVCAEMGTQVYISYPNSFEDPAKGKEQALAMYNEG
                     VDIIWQVAGGTGLGVFEAAADADKYTIGVDADQAKSFTGQPQLAQTIVTSMFKDCGAA
                     LVYAVEELIGGSYPAGTTKTMGLAEGTLGVVENDQYLEMVPEEIRKQVNDAAEKLKSG
                     EIVPYSVVAEPERWEEIKAAAMKG"
     gene            7230..7985
                     /locus_tag="ACZPFK_00740"
     CDS             7230..7985
                     /locus_tag="ACZPFK_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1404864.1"
                     /translation="MSNANDRQQQLLSLINSKGMLSVKAFSNLLGVSEMTIRRDLKHL
                     RGDGTSVPGDAPSSASHSEYNLLDELQKSNEQKTRIGKFAAGLISPEDVIIIDTGSTT
                     ARILPYIPADYPLTVLCFNANILDGIRHYNNINLLFCGGHYHRKTEMFESPEGLQFIK
                     GIRANKVFLSAAGVHRSLGITCANSYEVATKQAIIQSSQERILLADSGKFDAVRSAYF
                     GDLSEISTIVTDSNLSAEWAEYLRSQGIALHLV"
     gene            8084..8800
                     /locus_tag="ACZPFK_00745"
     CDS             8084..8800
                     /locus_tag="ACZPFK_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1404865.1"
                     /translation="MATPHNAAGKGDIAKTVLMPGDPLRAKFLAENFLEDAHCFNTVR
                     NMFGYTGKYKGKPVSVMGSGMGITSIGIYSYELFSFYDVDNIIRIGTCGCYADEKFEL
                     LDTVLITEAFSFSTYALEQSGFEGNVTHADPQLLERLRKSAADLGLRLPEARTHSSDV
                     FYYEENRSKNLVPRMRYEYGCDVIECECFALFHNAAVTGKHAAGLMSVVDLETRDLHA
                     TVEERERGGSQMMEIALGIL"
     gene            8846..9493
                     /gene="deoC"
                     /locus_tag="ACZPFK_00750"
     CDS             8846..9493
                     /gene="deoC"
                     /locus_tag="ACZPFK_00750"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1404866.1"
                     /translation="MNPKDILSRIDHTCLKPFATHTDIKKLCEEAVELGTASVCIPPC
                     FVDYAFQQFGDRLTICTVIGFPLGYQCTEVKLLETAKALRDGAAEIDMVINVGDAKEG
                     NFKKITEEIRSLKNLVGGDRVLKVIVETCYLTAEEKVALCRCVSEAGADYIKTSTGFG
                     PQGAVLEDITLFKEHIAPQVKIKAAGGIKTLEDMERFIDLGADRIGSSSAAAIFS"
BASE COUNT         2288 a         2811 c         2437 g         2175 t
ORIGIN      
        1 cgccggcgtc tcccggccgc cgccggaccc cgagaccgcg acggcgtcgc accgctccat
       61 ccccagccgc aggtcctccg cccagggccg ccccgacagg atcggctgct gctcgaaccg
      121 caccccgccg atcacccgga tctgcgaccc ggcgcggcag gcggccaatt gctcggcgta
      181 aggcgcgtcc tcctcccggc gcagctgccc cgccaccgag gcggcgaaga tgaactttgc
      241 accagagcgc cgcgccagct cccagctctt gaaaaagtcc ccgcccgcgc tgacccccag
      301 cggacagggc cagccccgca gcagctccag cgcggcggcc aggtcctgca gctcggcgcc
      361 gcggtcggcg atgagcaggg cgctcacccc gcagccggtg tagagctccg cttccctccg
      421 gcagcggtcc agcttttctt ttctgccgcc gccccgcagc cgcagcgccg cgatcaccgc
      481 cttggggccg caaagatcgt caggaaacat cgtcttcccc ctccccacgg ccggtgccgc
      541 gcgggtctgc cccgtgggcc ggcaaagctc gtccccttgt tgccctctcg cctcgggggc
      601 gcagctcttt tgcagcgaaa aagggcctat cggcccctgg gccgccgacc agctcgtccc
      661 tctcttttgc aacctcccct tgaggagcgt cgtgcaaagg agcaaaaaaa tggagaagca
      721 aggggattaa ggtctttcct atattttcca ttttttatta tacccgatcc cggccaaaaa
      781 gtaaaatttt ttgaggaaaa gctgcttttt taatcttgcc gtgatgcgag tgaaaaacgg
      841 tgtagggaca aaaaaacggc gcccgcaggc gccatttcca acttctctcc ttattccagt
      901 tttttaaacc tattactttt ctgcggcggt gcggggggat catttttcgg caaattcgga
      961 tgcagctggc aaatgcgccc gctcgcaatc ttgacaaata aaaaagccgc aaaagcggcc
     1021 ttttttatac tgggagtttt tctgcctggt aagctctgac acaggagaag atgacaatgc
     1081 cattcacaag gcagcccatt gcaagagcaa ggtacaacgc ctcaactgaa aaaaattcca
     1141 cgatcctcgc cgccccgggc tgaaacagga tgctgcccag gtttccgcca aataccatca
     1201 tagaataacc tgcggcagag ttttcgcgaa ccgaatgccc gccgataaac ataagattgg
     1261 caaaggcggg gctgatcatg atgccggtga agatcatcgc caacagcttt gcccaccccc
     1321 agggcgcaaa gaccagggcg agaagaccaa gacctcccag ggcaaagcag aaaggaaacc
     1381 atctaaaata gtttttgacc tttgtgctca gataggggca gatcataaag gcaagccagt
     1441 aggcggatat tccaatattg ccgattttct caaagccttg attcgccatc aaggtcacgg
     1501 tccagcaggt cactgtgctt tccacgccga gaaaaagaaa gacggcgatc atgcttctgc
     1561 gcagcgcttt atttttcaag gtctgcaaga tcgacaggga ggggcgtttt tcctgttttt
     1621 tggcaaagct gtctatcgag gacagtctca gaaatgaaaa ggtcagcgca gcgccaacca
     1681 tcaacaagat caggtacatc ggcttatagg catccgactt gagcccccag ctgatcagga
     1741 tcggggccag tacgcccccc agcccaagtg tgatctccgt caccccaaaa actttccgcg
     1801 ctttgctgtc gttgacgtcc tccagcaggg aaagcgtcag gctttccata ccaccgaaag
     1861 caaacccgca gagcaaaagg cctgccagca gcatataaaa atgggggctc atcagtatga
     1921 gcagtgaacc cgccatcagg gataccgttg ccgcgcaaag gccatacctt ttgcccacac
     1981 gctccgaaag ctcgcccaga aaaaccgcta caaagacaga accgccataa aaagtgctga
     2041 tcatcaaccc cagctggctc tccgtggcgc cccaggcgcg ggcaagattg ttcaccaccg
     2101 cttgggtacc cgcattcagc atcccgagaa aaagaaagat caaaacgccc aacagattta
     2161 attttttctt gctgctctcc atcttcccga ctcctcgcgc ggaaaggccg gtcttttttc
     2221 cttaccgctt tgctcttcaa aaatcgcttt atttgctcaa tttcttcttt ttgagataat
     2281 tcaccacaga aaatgcaatt accccaagca aaacgccaaa atagggaatc gttgtcagca
     2341 ggtcgctgga aacgtctgtc ctggacagca ctgcaacgat cgcctggaaa aaagagaaag
     2401 ccagggtcgt cacgctcaat ccaaggaaac tgcttccccc catggcctgg gcggcaaacc
     2461 cgatccagcc ccggccggca atcatatttc tcgaaaaagc ggagagatat cccattgtca
     2521 tataggcgcc tcccaaggcc gccagcagcc ctgagagcac aatggcactg acctgcattc
     2581 tgttgacgtt gatccccacc gatttggccg ccgccgggtt cagccctgcg gcttttagcc
     2641 gcaggccgta ggacgtcttg cccaccaaca gggggatgag gatcataaat gcaaagcaga
     2701 cataggtcaa aaggtactgg tctgaaaaaa cagcgcgcag gaaagggatc ttctccagcc
     2761 aggaaaccgt gacagtgggc agtaccttgg agggcagaga agcactgata cccttgttgc
     2821 cggaaaaaat gtacagaata aaaatggtga atgtcgccgt aaacgtgttg atcgcgatgc
     2881 cgaccaggaa cgggtccgtg ccaagcttca tcgtcataaa cgccagcagc agtgcgagca
     2941 ggatgccgga agccaccgcg atgagcagcc ccagcaacgc gctttgggtc caatagctgc
     3001 cgagcacggc catgagcgcg ctcatgagca tgatgccctc aatcgcaatg ttcaacaggc
     3061 cgctggtggc ggcgatataa gcgctcatcg acgcgaacat gagcgggacg ccgacacgga
     3121 tgatcgtggc cagaaaatcc tcgctcagga ccatggataa aacatcatgc atccgccatg
     3181 ccctccttaa gaagtttttt ctccctcagg ccccgaagaa aatactgaga ggagatcagc
     3241 agaaccaagg tggcttcgac gatggaaatg atctccaccg gcacgaccgt gctgcgggac
     3301 ataatttctg caccgatctt caaataactg atcccaaacg ccgcgatcat gaccgggacg
     3361 acccggttgt tgcccatcat ggcgatcagc atgcccgtgg ccccgtgggt cgtgatcttc
     3421 gtccaggtaa agcggtcata aaagctcagc agctccaccg aggagcccac acctgcaaga
     3481 aagcccgaga taaaatggat cagcaaaatc agggaaaagg cgccgatgcc ggaatatttt
     3541 gcaaaatttg cattttgtcc cgagatgcgt attttgtatc ccagccgtgt tttattcatc
     3601 atcacataga ccaaaacggc cgcgagcagg gccagaaaaa accccaccgt gatgcgggtg
     3661 ccggaaaaca aatacggcag cttggcgctt ttggcaaaca ggggcgaagc ggtgaccgag
     3721 atgtcagtcg ccagcatttt gtctttgagg agccacaatc ctatgcccag atagattgaa
     3781 ttgagcatca aggtggacac gacgatgttg gtatcgaact tcgcctttaa aaatccgggg
     3841 atcagcataa ggaggccgcc caccgtgccg gagagcaaaa tcgcgagcag aggatgcaaa
     3901 aaatggctgc tgagagcgag ctgcgtcgcc gcataggttg tgaccagccc gcagatgaaa
     3961 aatatccctt ccacaccgag attgaagatc cctgcccgaa acaggatcgc gcaggccagg
     4021 gccgtaaaga ccagcggcgt catcatttcg agcacgttgc tgaaatagcg gatcttggtc
     4081 agaggtgcgg tcagcatcgt cgcaaaagac tggagagggg cctcggcggt aaagcacagg
     4141 gcgataaacg tgatggccaa tgcaatcagc agggccgtca gggtcttgat cacgccaaaa
     4201 accttttcat cggacagttt caacttagcc acagagcgct ccctccagtt cttccggcgt
     4261 ttgttttttt acccccagca tgtacaggcc cagttcctgc agcgaaagcg atttaacatc
     4321 cttaaagtaa gcagagatgg tgccgttgta catgacgatc aagcggtcgc tgaggctgat
     4381 tacctcatcc aggtcggcgc tgatcaatag gacggcggcg cctctgttcc tcatctcgat
     4441 cagctgagtg cggatatatt ccgaactgcc gatgtcgatc ccctgggtgg gctgttccgc
     4501 aagcatacag accggatggg tattgtcctc acgtgcaacc acgacttttt gcatgttgcc
     4561 gccggagagg ttgctcactg ccgtatgaat ggagctggtg tggatggcaa atttggagac
     4621 cagcttttcc gcggtctcgg aaattttctt ttgatccata aaaaagcgcc cgttgatgtc
     4681 ctcgcggtca taatacgtcg aaataatgtt gtcgcggagg gatgcggtct cagcaacacc
     4741 gtccaccaag cggtcctccg ggacataaga gagccccaac agtcttcgct tctttacgct
     4801 cagggcctcg atccggcggc cgtccagcgt gatcttgccg ctgttcatgg cgccgatccc
     4861 cgcaagcgct cgcaccagct cggattgacc gttcccctgc acgcccgcga cgcccagaat
     4921 ctctccactc tgcagggaaa aagaaatgtt gtccagcaga gtctttgttt tgctgtgcag
     4981 ctcaagcccc tcaacccgca ggatttcccg gtgttccccg acatcctttt tcagcgcgtc
     5041 gtaggagctc agcagctttc tgcccacgat cagctctgcc aaccggttga gatccacctc
     5101 ttcgttgtcg taggtcccct tgccctcgcc gccgcgtatc acggtgatcc tgtcgctgat
     5161 ccgcttgacc tcattgagct tgtgggagat aaagatgatc gtgtgcccca tctcgcgcag
     5221 cttttgcagc tcctcaaaca gcgtctccgt ctcctgcggc gtcaggaccg aggtgggctc
     5281 atccaaaata atgatctccg cgtttcgata aagcgttttc agaatctcga ctttctgctt
     5341 ttctccggca gacaggtttt ccaccttgct cagcacgtcc acgtccagat gatagtgctc
     5401 gctcagctct ttcgtctgtc ttaccgcgct cttataatcc acgatgctgc gcagcttacg
     5461 gggctcgtcc cccacgatga tgttctgcac caccgacatg cccgggatca gcataaagtg
     5521 ctggtgcacc atgccgatac cggcctggat cgcttccatc gagccgcgga attttcgcgg
     5581 ctcgcccttg tggatgatct gcccgtcgga ggggctttca atgccgtaga tgatcttcat
     5641 cagagtggat tttcccgcgc cgttttcccc cacgatggaa tggatctccc cttttttaaa
     5701 ggagatggaa atattgcggt ttgccaccac gccgttgtga tagaccttcg tgatattttg
     5761 cagctctaat atcgtctctt tcactttact ttaccctttg ttgttatttg ccggccgcag
     5821 gtcccccgtc ggctctcgac ggcaactttc cctttcagcc tttcatggca gcggccttga
     5881 tctcctccca gcgctcaggc tcggccacca cactataggg gacgatctct ccgctcttta
     5941 acttttcagc cgcgtcattg acctgcttgc ggatctcttc gggcaccatc tcaagatact
     6001 ggtcgttctc gacaacgccc aatgtgccct ctgcaaggcc catggtcttt gtcgtgcccg
     6061 ccggatagct gccgccgatc aactcctcca ccgcatagac cagggcggcg ccgcagtctt
     6121 tgaacatcga agtgacgatg gtctgtgcaa gctgaggctg gcctgtgaac gattttgcct
     6181 ggtctgcatc caccccgatg gtatatttgt ccgcatccgc cgcggcctca aagaccccaa
     6241 ggcccgtacc tccggccact tgccagatga tgtccacgcc ctcgttgtac atggccagag
     6301 cctgttcttt tcccttggcg gggtcctcaa aagaattggg atagctgata tagacctgag
     6361 tgcccatctc agcgcacacc tggcaatagc tgccgatgaa gtcgttcatg tattcgctgt
     6421 cctcaccaac aatgacaccc accttgtttt ccggatttgc tttctccatg ccgctggtcg
     6481 tgacaagtga gctcagcgtg cccgcgagat atcccagctc gctgagcgag taagtgacgg
     6541 caaaaacatt gtcaaggtct tcatgtgtgc tataatcaaa gttgaaaaac agctgatcgg
     6601 gatacatctt cgcagcgctg tacagcgtgg gttccacgtc cgcatacccg gaaacaatca
     6661 gatcataacc tgattcgcag gcatccatga aatagctctc ccattgggcc tggtccgtgc
     6721 cgatctcgat cagggtggcc tcaatctctt cgcgggcgtc cagcgtcttg cggcccgcct
     6781 ccacagcgtc gtaataggat tgatcgccga tatagggcag caggacggcc actttccaca
     6841 ccttttctcc cccggccact ttctcagggc cgcaggctgc cagcgacagg gtaaggatca
     6901 gcgctaaaaa aaccgaaaga acttttttca tcacaatttc cgcctttctt tcactcagct
     6961 tactatccat ccgaacattt ttgaggttcg gactgaatcc gaaaaacact ttagagccgc
     7021 ttttacatca cagggaacct ctctgtttcc ttttttgcac atttcacaaa acattttcaa
     7081 acatttgttt tttgtgctgt gtgtttttta taaaaataac acagtaatga aaaactgtca
     7141 acattaaatt atcaaaatgt tattttatta tttacatttg attggtttta acgtattata
     7201 ttgttatata ctggtaagga gtcataaaaa tgagtaatgc gaatgaccga cagcagcagc
     7261 tgttaagcct catcaactca aaggggatgc tctccgtcaa ggccttttct aacttgctcg
     7321 gcgtctccga aatgacgatc cggcgcgacc tcaaacatct gcgcggcgat ggaacctctg
     7381 ttccgggcga cgccccgtcc tctgcatccc attctgaata caacctcttg gatgagcttc
     7441 aaaaaagcaa cgagcagaaa acgcggatcg gaaaatttgc cgccggtctg atcagccctg
     7501 aggacgtcat catcatcgac acaggcagca ccaccgcgcg catacttccc tatatccccg
     7561 ccgattaccc cctgacggtt ctctgtttca acgccaatat tttggacggt atccgccatt
     7621 acaacaatat caatctgttg ttttgcggcg gccattacca tcgcaaaacc gaaatgttcg
     7681 agagccctga gggcctgcag ttcatcaaag ggattcgcgc aaacaaggtc tttctctccg
     7741 ccgccggcgt tcaccgctcg ctggggatca cctgcgccaa cagctatgag gtggcgacca
     7801 aacaggcgat catccaatct tcacaagagc gtattctgct ggcggactcg ggcaaattcg
     7861 atgcggtgcg ttccgcctat tttggggatc tgagcgagat ttccaccatt gtgaccgaca
     7921 gcaatctctc tgccgaatgg gcggagtatc tccgctccca gggcatcgct cttcacttgg
     7981 tatagaagcc aatcaaacat tttgttttat tttttaacaa taatgttgac ataaataaaa
     8041 cataatgtta ttatgagaaa aattcttacc aaggaggtcc actatggcca caccacacaa
     8101 cgccgccggc aagggggata tcgctaagac ggtcctgatg cccggcgacc ctctgcgcgc
     8161 aaaattttta gcagagaatt ttctcgagga tgcgcattgt ttcaacacgg tcagaaatat
     8221 gttcggctat accggcaagt acaaaggtaa gccggtctcc gtgatgggca gcggcatggg
     8281 catcacctca atcggcattt attcctacga gctattttcc ttttacgatg tggacaacat
     8341 catccgcatc ggtacctgtg gctgctacgc ggacgaaaag tttgagcttt tggacaccgt
     8401 cttgattaca gaggctttca gcttttcaac ttatgccctg gaacagagcg gcttcgaggg
     8461 aaatgtcacc catgccgacc cgcagctttt agagcggctg cgcaaaagtg cagccgacct
     8521 cgggctgcgg ttaccagagg cacggaccca ttccagcgat gtattttatt atgaagaaaa
     8581 ccgcagtaaa aacctcgtcc cgcgcatgcg ctatgaatac ggctgcgacg tgatcgaatg
     8641 cgagtgcttc gctctcttcc acaatgccgc cgtgacggga aaacacgcgg cgggtctgat
     8701 gtccgtggtc gacctggaga cccgcgacct ccacgccaca gtcgaagaaa gagaacgcgg
     8761 cggctctcaa atgatggaga ttgcgcttgg catcctttga cgggaaagtc agcccttttc
     8821 catctcaaag aggaggaatt catttatgaa cccaaaggat attttaagcc gcatcgacca
     8881 cacctgcctc aagcctttcg caacgcacac cgacatcaag aagctgtgtg aagaggcagt
     8941 ggaactcggg accgcttcgg tatgtatccc cccctgtttc gtggactatg cttttcaaca
     9001 atttggggat cgccttacca tctgtacggt aatcgggttt cccctgggat atcagtgcac
     9061 ggaagtcaaa cttttggaga ccgcaaaagc gctcagggac ggggccgcag aaattgatat
     9121 ggtgatcaac gtcggcgacg ccaaagaggg aaactttaaa aagatcacgg aagagattcg
     9181 ctcgctgaaa aaccttgtgg ggggcgatcg ggttctaaaa gtgatcgttg agacctgtta
     9241 cctcaccgcg gaagaaaagg tggccctttg ccgttgtgtg agtgaagccg gcgccgacta
     9301 catcaaaaca tccaccggct tcggccctca aggggcggtt ctggaggata ttacgctttt
     9361 taaggagcat atcgcccctc aggtcaagat caaagctgcg ggcggtatca agaccctgga
     9421 ggatatggag cgttttatcg atctcggggc cgaccgcatc ggcagcagct cggctgccgc
     9481 catcttcagc tgacaggcgt ctctttcttt ggaacccgga cgttttaaag agaaagcccg
     9541 tgtccctttc accggcacag ctgtatttct gtccgcagca tccccttttc cggacagaaa
     9601 agggagtcgc atcttcgctt ttttctgaat ttcctaaaaa aacaactgat ccttgatggc
     9661 cccgtttctc gccgctgcgc ggcaaccgaa acggatggcc aaaaaaatag c
//
LOCUS       DBMDYA010000014         9549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1960_length_9549_cov_1.560119, whole genome shotgun sequence.
ACCESSION   DBMDYA010000014 DBMDYA010000000
VERSION     DBMDYA010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_9549_cov_1.560119"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(72..1490)
                     /locus_tag="ACZPFK_00755"
     CDS             complement(72..1490)
                     /locus_tag="ACZPFK_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404867.1"
                     /translation="MRSPDLILLSLTNLSRQKSRTTFTVIGVVVGTCAIVLMISLGIA
                     VNRQNEAMLAPSGDLTKITIYNYSNGGKEKAMDDKALSQIRAKKNVVSATPFWQADSL
                     NGTVLAGKRNRYSSYAGNIYGVYPDSLEALGIKLESGVYLTDDTAMTKKVIPVLVGQN
                     FGYSFEDTKRRESSAQRTRYKGQTNAKGEELPPFVKVDTDKMTLALTVTDDKTDKQKT
                     VLTYNLKVVGVMASNYGIYYATDNGILMRLGDLQKLEQQYQKAAGLRRQKKKAINYDE
                     VYVKCANMDAVAPIEEWIHKDMGFENTNSMTDARKNMQQSVAKTQMILGGLASISLLV
                     AMLNIMNTMTMAVSERRKEIGVLKVLGCKLNDIRIMFLLESGMIGFLGGLIGIGISLA
                     ASWFLNHLPAIMALFGNSIDLSWITAAMGGGGFGTGEISIIPLWLILAALLFASLVGL
                     LSGLAPANKAVKISALQAIKSE"
     gene            complement(1532..3352)
                     /locus_tag="ACZPFK_00760"
     CDS             complement(1532..3352)
                     /locus_tag="ACZPFK_00760"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404868.1"
                     /translation="MAKNQKTPGILHEDIPRNTKETAKRLLSRLGRQKRKLVVIVVAT
                     LLSSAAFTALPLAVGRGIDTLVTAIRTYDGSVGIVETALQVLWLPVVLIAASALVSGL
                     LSYVQQYLIASVGEELTLSFRRDISAKLHRLPLRYFDSHKTGEVMSRVTNDLEKVSAA
                     MQEGLPQFVSSVFTILFAAITMLVLNPPLFGVVVIALIVSMVATGYVSTLAQRAYGEN
                     LASLGALTGKIEEIYAGNRVVKVFNQQRAMLAAAQQLNERQYRAQRNAQFADFAIYPT
                     IRALGQLGFIVTAVLGGGMALSGAITLGTVQAFLQYVNQISEPVTETAYVVTSLQAAI
                     AGAERVFALLDEEEERPDGHGSAAGITQGHVIFRDVRFGYTPEKTLIQNLDLEVRPNE
                     MVAIVGPTGGGKTTLVNLLMRFYELDGGAITIDGQDITALPRCEMRRHIGMVLQDTWL
                     FEGTIAENIAYGRRDASREEIVTAAKAACCAHFIRTLPQGYDTVLSGEAAGVSQGQMQ
                     LLTIARAMLTDPAILVLDEATSSVDTRTEVEIQKAMARLMRGKTSFVIAHRLSTIQNA
                     DLILVVRDGDIVERGSHAELLKKNGFYAGLYHSQFEAKAS"
     gene            complement(3356..5083)
                     /locus_tag="ACZPFK_00765"
     CDS             complement(3356..5083)
                     /locus_tag="ACZPFK_00765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404869.1"
                     /translation="MTYLKQFLMRHKGKTALAVLLLLAQVVGTLLIPALVADIVDRGI
                     LRGDMAAVLRIGGWMLAVTVLSTGVAAWGSWVTSDLASLFGFEMRSRLLRKSQQLSLQ
                     QFDAVGISSMITRTTSDIINLQRTMGMVLQMVVPAPILVGISIVMTTLVSPVMAAIQL
                     IFMAVLLVLTAVILKKSNALSRSIQTKLDRINQVVRETVTGVRVIRAFGNEAYEEKRS
                     GAAYEDYADNMIRLNRLFAVITPVVWLLMGALMAVILGVGGALSLGGGMAVGQITAVA
                     EYSTLTMAYLIMAASVFTTLPKARACLGRLRELLDTKPAVCDAPIPHTAVRPASATVE
                     FDHVTFSYEGAEEPVLRDLSFTLRPGQTTAVIGSTGSGKSTLADLLLRLHDIQGGRIL
                     LGGEDVRDFTQQELRDRIACVPQKAFLFGGTIAENLRMGRVHATDEELWEALRIAQAE
                     DFVRRLPGELDTPVAQGGTNFSGGQRQRLAIARALVKRADVFIFDDSFSALDVRTDAA
                     LRRALLERVTAPAKLIIAQRVNTILDADQILVLDQGRLVGHGTHRELLERCPTYREIA
                     ESQMQKKEA"
     gene            5191..6000
                     /locus_tag="ACZPFK_00770"
     CDS             5191..6000
                     /locus_tag="ACZPFK_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1404870.1"
                     /translation="MSQKKQLSILEFSRLTGISRDNLRFYDRIGLLKPEARGENGYRY
                     YTRRQLGSAYLVGSLRLLGVGIEDIRRYSAGRTPEELLALFAEQEARIQAEIETLRET
                     SRIMQLYADMAREALNRGEGGFLVRERKRERIFLCPPFPAARDEDEAELLAYEYAGDH
                     GVNLGCPMGVMVSRETLLAGAGGPTYRYYLKSCRQGNAWKPGGLYAVAYGKSEAPDTG
                     DAYRQLLGFIREQGLEVAGDAYGEYLLDDLAVQTPEQYYGRIEILVKAIKK"
     gene            complement(6494..6569)
                     /locus_tag="ACZPFK_00775"
     tRNA            complement(6494..6569)
                     /locus_tag="ACZPFK_00775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6534..6536),aa:Ala,seq:ggc)
     gene            6782..8164
                     /locus_tag="ACZPFK_00780"
     CDS             6782..8164
                     /locus_tag="ACZPFK_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1404871.1"
                     /translation="MARLNDLGRDKVGGLVLRLAIPAMLAQFVNVLYSIVDRMYIGNI
                     PGVGGLALAGAGVCGPIVTLISSFAFLVGIGGAPLLAMRMGEGNHQGAQDILANCFLM
                     LLGLSAGLMGLFLAAKEPLLHWFGASEATYPYADSYLTIYLLGTVFAILSVGLNQFIV
                     CQGFSTIGMATVLIGAVSNIILDPIFIFVLDKGVAGAAAATVLSQAASCAFTLLFLFG
                     GRTRVRITFRGYDLRIMRRVLTFGVSPFLITASDSVILIALNSVLQKYGGPQSGDMYI
                     TCATIVQSYMQLITMPLGGITGGTQPVLSFNYGAKKIDRIRSGFRSILGLCAAFNSVM
                     LLISQFTPQVFVRIFTQDPQYTALSVWGIRVFTCGILVLAFQYTFVDGLTALGIARIA
                     VPLSLLRKGLFFGATMLLPPLFGVKAAFFAEPIADVMGGIISSIVFALVIGKILKRRE
                     EMPDGVALYE"
     gene            complement(8260..8760)
                     /locus_tag="ACZPFK_00785"
     CDS             complement(8260..8760)
                     /locus_tag="ACZPFK_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR1404872.1"
                     /translation="MWNEENQAAFRELIAPLLQAEEVQAMRGISQHAEGVSCYEHCVF
                     VSYVSFLLARRLGLNCRAAARGGLLHDLYLCDWSKTDVGKFERLVIHPLMALQNAEKF
                     GLNDLERDIIVKHMWPLTRALPRHRESFVVSLADKLCATAEMLHVYKAARVGQRLVFA
                     PAAENA"
     gene            complement(9101..9442)
                     /locus_tag="ACZPFK_00790"
     CDS             complement(9101..9442)
                     /locus_tag="ACZPFK_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013652.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1404873.1"
                     /translation="MMRIEPVPEILAHYKDYIEENRVKMVLPDQDVLNALYHDRILQL
                     NPLVYNLSEKFLAHHNLNLKNQPVDLDWVRANTVFVHYCGRNKPWKPGYLGVLDTFYR
                     HFQEELAALSS"
BASE COUNT         1837 a         2753 c         2859 g         2100 t
ORIGIN      
        1 atcaatgaaa tctctataga atcgtttttg gcgggcagca ctgcccaatg gcgggccttt
       61 tctgtgggag cctattcgct cttgatcgct tgcagggcac tgattttcac tgctttgttg
      121 gcgggagcga gcccggacag caggccaacc aaggaggcga acaaaagcgc ggccagaatc
      181 agccacaagg ggatgatgga aatttctcct gtgccgaagc cccctccgcc catggctgct
      241 gtgatccacg acaagtcaat agagtttccg aatagtgcca tgatcgcggg gagatgattc
      301 aggaaccagc ttgccgctaa actaatgcca atgccgatga gcccgcccaa aaaaccgatc
      361 atcccgcttt ccaacaggaa cattatacgg atatcgttca atttacagcc aagcactttc
      421 agcacgccga tttcttttct ccgttcgctg acagccatcg tcatcgtgtt catgatgttc
      481 agcatggcta cgagcaagct gatggacgca aggccgccca ggatcatctg ggttttagca
      541 acgctctgct gcatgttttt gcgggcatcc gtcatcgaat tggtgttttc aaatcccatg
      601 tccttatgga tccactcttc aatgggagcc accgcgtcca tattggcgca tttcacatat
      661 acttcgtcgt agttgatcgc ctttttcttt tgccgcctca ggccggccgc tttttgatac
      721 tgttgttcca gcttttgcaa atcacccagc cgcatgagaa tgccgttatc ggttgcataa
      781 tagatgccat aattggaagc catgacaccg actactttca ggttgtacgt caggacggtt
      841 ttttgcttat ccgtcttatc atccgtaacc gtgagggcca aggtcatttt gtctgtgtca
      901 accttgacaa aaggggggag ctcttctcct ttcgcatttg tctgcccttt atagcgcgtt
      961 cgctgagcac tgctttccct gcgttttgta tcttcaaaag aatagccgaa attctggccg
     1021 accagtacgg ggatcacctt cttggtcatc gccgtgtcat ccgttaaata cactccggat
     1081 tccagcttga tccccagtgc ctcaagagaa tccgggtata ctccgtagat attcccggca
     1141 tagcttgaat aacgatttcg cttgcctgca agaacagttc cgttcaaaga atctgcctgc
     1201 cagaatgggg ttgcggatac cacatttttc ttcgcacgga tttgggataa agccttgtca
     1261 tccatcgctt tctccttgcc cccgtttgaa taattgtaga tggtgatttt cgtcagatcg
     1321 cctgacggcg ctaacatcgc ttcattttgt ctgtttacgg cgatcccaag gctgatcatc
     1381 aaaacgatgg cacaggttcc aaccacgaca ccgatcacgg taaacgtggt tctgcttttc
     1441 tgtctgctca aattggtaag ggataaaagg atcaaatccg gtgatctcat attttagcct
     1501 catatctttt ttggatgccg ccgctttttt tctaggaggc ctttgcctcg aactggctgt
     1561 gatacaagcc cgcgtagaag ccgtttttct tcaatagctc ggcgtggctg ccccgttcca
     1621 caatgtcgcc gtcccgcacc acaaggatga ggtcggcgtt ctggatggtg gacaggcggt
     1681 gggcgatgac gaagctggtc ttgccccgca tcagccgggc catggccttt tgaatttcca
     1741 cttcggtgcg ggtgtccacg ctggaggtgg cctcgtccag caccaaaatg gccgggtcgg
     1801 tgagcatggc acgggcgatg gtgagcagct gcatctgccc ctgggagacc ccggcggcct
     1861 cgccggacag gaccgtgtcg tatccctggg gaagtgtgcg aatgaagtgg gcgcagcagg
     1921 cggccttggc ggcggtcacg atttcttccc ggctggcgtc ccgcctgccg taggcaatat
     1981 tttccgcgat ggtgccctca aaaagccagg tgtcctgcag caccatgccg atatgccgcc
     2041 gcatctcgca gcggggcagg gcggtgatgt cctgtccgtc gatggtgatg gcgccgccgt
     2101 ccagctcgta gaagcgcatc agcaggttca caagggtggt cttgccgccg ccggtggggc
     2161 ccacaatggc caccatctcg ttggggcgca cctccaaatc aaggttctga atcagcgttt
     2221 tttcgggggt gtagccgaag cgcacgtccc ggaaaatcac gtgcccctgg gtgatgcccg
     2281 ccgcgctgcc gtgcccgtcg gggcgctcct cctcctcgtc cagcaaggcg aagacgcgct
     2341 cggcccccgc gatggcggcc tggagagagg tgaccacgta agcggtctcg gtgacgggct
     2401 cggagatctg gttcacatac tgtaaaaatg cctgaacggt gcccagggtg attgccccgc
     2461 tcagggccat accgccgccc aggacggcgg tgacgataaa gcccagctgc cccagcgccc
     2521 ggatggtggg gtaaatggcg aaatcagcga actgggcgtt tcgctgggcg cggtattgcc
     2581 gctcgttgag ctgctgcgcg gccgccagca tcgcccgctg ctggttgaac accttcacca
     2641 cccggttacc ggcataaatc tcctcgatct tgccggtcag ggcccccagg gaggccagat
     2701 tttccccata ggcccgctga gccagggtgg agacataccc ggtggccacc atgctgacga
     2761 tgagggcgat cacgaccacg ccgaagaggg gcgggttcaa taccagcata gtgatggcgg
     2821 cgaacaggat ggtgaacacc gacgagacga actgcggcag cccctcctgc atggcggcgg
     2881 aaaccttttc cagatcgttg gtcacccggc tcatgacctc gccggtctta tggctgtcga
     2941 agtagcgcag cggcagccgg tgcagcttgg cgctgatgtc ccggcggaaa gaaagggtca
     3001 gctcctctcc cacgctggcg atcaggtact gctgaacata ggacagcagg ccgctgacga
     3061 gcgccgaggc ggcgatgaga accaccggca gccagagcac ctggagagcc gtctccacga
     3121 tcccgacgga gccgtcatag gtgcgaatgg ccgtcaccag ggtgtcgatg cccctgccca
     3181 cggccaaggg cagagcggta aaggcggccg agctgagcag ggtggccacg acgatgacga
     3241 ccaattttcg tttctggcgg cccagtcggc tcaaaagccg cttggcggtt tctttggtgt
     3301 tacgggggat atcctcatgg agaatccccg gtgttttctg atttttcgcc atggtttacg
     3361 cctccttttt ctgcatttga ctctcagcga tctcacggta ggtggggcag cgttccagca
     3421 gctcccggtg cgtcccgtga cccaccaacc gcccctggtc cagcaccagg atctggtctg
     3481 cgtccaggat agtgttcacc cgctgggcga tgatgagctt ggcgggcgct gtcacgcgct
     3541 cgagcagggc cctgcgcagc gcggcgtccg tccgcacgtc cagcgccgag aagctgtcgt
     3601 cgaagatgaa cacatcggcc cgcttcacca gcgcccgggc gatggccagg cgctggcgct
     3661 gcccgccgga aaagttggtg ccgccctggg ccacaggggt atccagctcc cccggcaggc
     3721 ggcggacgaa gtcctccgcc tgggcgatgc gcagggcctc ccacagctcc tcgtccgtgg
     3781 cgtggacccg gcccatgcgc aggttttcag caatggtgcc gccaaacagg aacgcttttt
     3841 gaggcacgca ggcgatccgg tcccgcagtt cctgctgtgt gaaatcgcgc acgtcctcac
     3901 cgcccagcag gatgcgcccc ccttggatgt cgtgcagccg cagcagcagg tcggccaggg
     3961 tgcttttgcc cgatccggtg ctgccgatga cggcggtggt ctggccgggg cgcagtgtaa
     4021 aggacaggtc gcgcagcaca ggctcctcgg ccccctcgta agaaaatgtc acgtggtcga
     4081 actccaccgt ggcggacgcg ggccggaccg ctgtgtgcgg aatcggcgcg tcgcacacgg
     4141 cgggcttcgt gtccagcagc tcccgcaggc gccccagaca ggcgcgggcc ttgggcagcg
     4201 tggtgaacac cgaggccgcc atgatgaggt aggccatagt cagggtggag tactcggcca
     4261 cggcggtgat ttgtcccacc gccatgccgc cgcccagcga gagggccccg cccacgccca
     4321 ggatgaccgc catcagcgcc cccatcagca gccacaccac tggggtgatg accgcaaaaa
     4381 gccgattgag gcggatcatg ttgtccgcgt agtcctcgta ggccgcgccg gaccgttttt
     4441 cctcataggc ctcgttgccg aaagcgcgga tgacccgcac gccggtgacg gtctcccgca
     4501 ccacctggtt gatgcggtcc agcttggtct ggatgctgcg ggagagggcg tttgattttt
     4561 tcaggatcac ggcggtcagc accagcagca ccgccataaa tatcagctgg attgccgcca
     4621 tgacggggct gaccagggtg gtcatgacga tggagatccc caccaggatg ggcgcgggta
     4681 ctaccatctg cagcaccatg cccatggtcc gctggaggtt gatgatgtcg gaggtggtgc
     4741 gggtgatcat ggaggagatg cccaccgcgt cgaactgctg cagcgagagc tgctggcttt
     4801 tgcgcagcag cctggagcgc atctcgaacc cgaagaggga ggcgaggtcg ctggtgaccc
     4861 agctgcccca cgccgcgacg cccgtggaca gaacagtgac ggccagcatc caacccccga
     4921 tgcgcaggac ggcggccatg tcgccacgca ggatgccgcg atccacgatg tccgccacca
     4981 gcgccgggat gagcagcgtg cccaccacct gcgccagcaa cagcagcacg gccagggccg
     5041 ttttgccctt gtgccgcatg aggaactgtt tcaaataagt catgacaatc tcctttgccg
     5101 gtcttgcatt tcttttcgac aaagggtatc ataaacctta aagtcgctcc aatgtcaact
     5161 cttaaactgt gaattttttg tgaggcgctt atgagccaga aaaagcagct ctccattttg
     5221 gagttctccc gcttgacggg gatcagccgg gataatctgc ggttctatga ccggatcggc
     5281 ctgctcaaac cggaggcgcg gggagaaaac gggtatcgct actacacccg ccgccagttg
     5341 gggtccgcct atctggtcgg cagcctgcgg ctgctggggg tgggaataga agatatccgc
     5401 cgctactcgg ccgggcgcac acccgaggag ctgctggccc tgtttgccga gcaggaggcg
     5461 cgcatccagg cggagatcga aacgctgcgg gagaccagca ggatcatgca gctctacgct
     5521 gatatggcgc gagaggccct gaaccgcgga gagggaggat ttctggtgcg ggagcgcaag
     5581 cgggaacgga tcttcctctg tcccccattc cccgccgccc gggatgaaga cgaggcggag
     5641 cttcttgcct atgagtacgc cggcgatcac ggcgtcaacc tggggtgtcc catgggggtg
     5701 atggtgtcca gggaaacgct tttggccggt gcgggggggc ccacttatcg gtactacctc
     5761 aagtcttgcc ggcagggaaa cgcctggaag ccgggcgggc tctatgccgt cgcctatgga
     5821 aaaagcgaag ccccggacac gggggacgcc tatcggcagc tgctgggttt catccgagag
     5881 cagggtctgg aggtggccgg tgacgcctac ggggagtatc tgttggatga cctggccgtc
     5941 cagaccccgg agcagtatta cggccgcatc gagattttgg tgaaagcaat taaaaaatga
     6001 ttatacaaaa tcgtaaagat aaactatcct ttcaggggat gaaacccccg ggcctgtttt
     6061 ctcattcggg cagcgcgagc aattgtcaca atgtataaat gggatgcggc agattttatg
     6121 ttttgacgaa tagctttttt atccgggccg cgcaaggggc tggaaccatg ctgcaaaggt
     6181 gaaatgagga ttatctgaat cgcaatgcca ttccacgggg gtttcacacg cgggcaagtg
     6241 ccccgcttta ttcgcttcgg caagcgcgat ttctacacct ttcttttttg ttctcaaatc
     6301 atcggcaaag ccgctcaatc ccgcggttta aataaaaaaa cagcctacgc gttgcgccgg
     6361 ctgttttttg taaccatgcg cttcggttgt cacctcttgc gaggtgacaa ccgaagcgcg
     6421 gggaattgga atcgtttgtt ttttattgcg gaagcaataa aaaaagtcag accatttggt
     6481 ctgacttttt ttgtggtgga gatgaggggg atcgaacccc ttacctcttg aatgccattc
     6541 aagcgctctc ccaagtgagc tacaccccca cgggtttgcc ggtctctcga ctgctcgatt
     6601 attatagcaa acttgtatcg cagttgtcaa cacctgattt taaaaaaatt tccatttttg
     6661 cgcggacgtc ccggcgaggg gggaaaaaag atttttcttg ctctttcggg cgccatttgc
     6721 tataatcggt tccggcatcg ccgtgcggcg atgccggatt ttttgtgtaa ggggcagtga
     6781 aatggcacga ctcaatgatc tggggcggga taaggtcggc ggcctggtgc tgcggctggc
     6841 catccccgcc atgctggcgc agttcgtcaa cgtgctgtac agcatcgtgg accggatgta
     6901 tatcggcaat atccccgggg tgggcggcct ggcgctggcc ggggcggggg tctgcggccc
     6961 catcgtcacc ctgatctcct cctttgcctt tctggtgggg atcggcggcg cgccgctgct
     7021 cgccatgcgc atgggggagg gcaaccacca gggcgcgcag gacattttgg ccaactgctt
     7081 tctgatgctg ttgggcctgt cggccggcct gatgggcctt tttttagctg caaaggagcc
     7141 gctgctgcac tggtttggtg ccagcgaggc gacctacccc tatgcggaca gctacctcac
     7201 catctatctg ctgggcacgg tcttcgccat cctgtcggtg gggctcaacc aattcatcgt
     7261 ctgccagggc ttttccacga tcggcatggc gacggtgctc atcggggcgg tgtccaatat
     7321 catcctggac cccatcttta tttttgtgct ggacaagggg gtcgcgggcg cggcggcggc
     7381 gaccgtgctc tcccaggcgg cgagctgtgc ctttacgctg ctttttctgt tcggggggcg
     7441 cacccgcgtg cgtatcacct ttagggggta tgacctgcgc attatgcgcc gggtgctcac
     7501 gttcggcgtt tccccgtttc tcatcaccgc ctcggacagc gtcatcctca tcgccctcaa
     7561 cagcgtattg cagaagtacg gcggcccgca gagcggggat atgtacatca cctgcgccac
     7621 catcgtgcag agctatatgc agctgatcac catgccgctg ggcggcatta ccggcggcac
     7681 ccagccggtg ctgagcttta actacggggc gaaaaagatc gaccgcatcc gcagtgggtt
     7741 tcgcagtatt ttggggctgt gcgcagcgtt caacagcgtc atgctgttga tctcgcagtt
     7801 taccccgcag gtgtttgttc gcatttttac ccaggatccc caatatacgg ctctctccgt
     7861 ctggggcatc cgcgttttca cctgcggcat tttggtgctt gcctttcaat atacctttgt
     7921 ggacggcctg acggcgctcg gcatcgcccg tatcgccgtg ccgctgtcgc tgctgagaaa
     7981 aggattgttt ttcggggcca cgatgctgct gccgcccctc tttggggtca aggcggcctt
     8041 ttttgccgag cccatcgccg atgtgatggg cggtatcatc tccagcatcg tctttgccct
     8101 ggtcatcggc aagatcctga aacggcgcga ggagatgccg gacggcgttg cgctgtatga
     8161 atgagaaacc gcaaggcgta aaaaagggaa ccgaagagat ccggttcctt ttttattttg
     8221 tttttccgcc cacctgccca cgcccctcaa gggatgcgct caggcgtttt ctgcggcggg
     8281 tgcaaagaca agacgctggc ccacccgggc ggccttgtag acgtgcagca tctccgccgt
     8341 ggcgcagagc ttgtcggcaa ggctgaccac aaagctctcg cggtggcggg gcaaggcgcg
     8401 ggtcaggggc cacatgtgct tgacgatgat atcccgctca aggtcgttca gaccgaattt
     8461 ttccgcgttt tgcagcgcca tcagggggtg gatgactagg cgctcgaact tgcccacatc
     8521 ggtcttgctc cagtcgcaga ggtacaggtc gtgcagcagg cctccgcggg cggcggcgcg
     8581 gcaattcagc cccagacggc gggccagcaa aaagctgacg taagagacaa aaacacagtg
     8641 ctcataacag ctgacgccct cggcatgctg cgagatcccg cgcatggcct gcacctcttc
     8701 ggcttgcagc aaaggggcga tcagctcgcg gaacgcggct tgattttctt cattccacat
     8761 gggaatcatc tccaataaga tagtggtccc gtttcggggc gcatccgcac aggggcgctg
     8821 cggcgcaagc gggcttccga ccgccgcgct gaaaaggggc cggcgggacg gctttggacg
     8881 gcgattgaaa catatctcta ggcaaagttt ctccgccgtc ctcaccatta tacgataagg
     8941 ggaaggattt gtcttccggt ggggcaaagt ttcacacaaa tatttacagt ttgatgacaa
     9001 aaagcaaaag ccggcctggg tgaacgcgct cttttgtcgc atttatccat aaagcgaggg
     9061 ggcgcaaacg accgctcgct cgtgcctctc tgggcacgtt tcaggatgaa agggccgcca
     9121 gctcttcctg aaaatgacgg tagaacgtgt ccaaaacccc aagatacccc ggcttccagg
     9181 gcttattgcg gccgcagtag tggacaaaaa cggtgtttgc ccgcacccaa tccaggtcca
     9241 ccggctggtt ttttaggttc aggttgtggt gggcgagaaa tttttcagag aggttgtaaa
     9301 ccagggggtt caactgcagg atgcggtcgt ggtagagcgc gttgagcacg tcctgatccg
     9361 gcagcaccat cttgacccgg ttttcttcaa tataatcttt ataatgggca agaatctccg
     9421 gcaccggttc tatccgcatc atgttggcgt cttttcctta gatgatgccg acggcagtga
     9481 ggatggcctc atggtatttg gctgttttca tagggccttt gatcaaagta tttttcggat
     9541 aaagacgga
//
LOCUS       DBMDYA010000015         9471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1976_length_9471_cov_1.591676, whole genome shotgun sequence.
ACCESSION   DBMDYA010000015 DBMDYA010000000
VERSION     DBMDYA010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1976_length_9471_cov_1.591676"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..1539
                     /locus_tag="ACZPFK_00795"
     CDS             121..1539
                     /locus_tag="ACZPFK_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1404874.1"
                     /translation="MDKYKRLVSNTVLFAISSFSSKILSFLLMPFFTRMFGLGEFGDA
                     DLITKYAQLLLPIVSVGIANAVIRYGLDKTYNKASVFTGGLIAEGVGIAVFAACWPLV
                     ARIPDMGGYTALLYAYVVMSILRNLCSQFVRAKQYTRLYAVDGVLNTVLYLFFIVLFI
                     AGFKWGIAGYVLATAAADFCSALFLFFTARLYRYIRISRFDRPLLREMLRYAAPLIPT
                     SMFWWITNTSDHMFITSMLGSEANGLYVAAYKVPNVIMLFSTLFTEAWQLSAVTDGGR
                     NSPGRAKFFSQVFLSYQSLLFIVAGGLIYCCKFVMMVLTLSGKSAFNAAWQYIPLLVV
                     ATVFSCFVTFLGSVYMVEKRSGESFFTMLVGAVLNLALNWFLIPVWGPNGAAFATFAS
                     YLLVFVIRAFSTRRYIAMDLHLGRMTVTLALLAGEIALMLSEAKYWQLWCGLLCAAVV
                     AFNFWPILQTVRRLLARRRRRS"
     gene            1574..2284
                     /locus_tag="ACZPFK_00800"
     CDS             1574..2284
                     /locus_tag="ACZPFK_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404875.1"
                     /translation="MRVLFVGNSHTYFNDMPHTFARLCAAAGAGQPQTVLLAHSARPL
                     GWHLEEYFELRYNLLYGGYDYCVIQQQAHPFPGEEETARDCGRIIGLCRAAGARPVLL
                     MTWAERAAPEHQQAMTRAYRGLAASTGALLAPVGEIWQQLRRERPEIELFWRDGEHAS
                     PLGDYLVAVTLCAVLTGADPEALPGLGADFAGEAEIAFFDHPRVREDPACIETPLDPA
                     ACAAIRRAVKRACAAKAP"
     gene            complement(2451..4259)
                     /locus_tag="ACZPFK_00805"
     CDS             complement(2451..4259)
                     /locus_tag="ACZPFK_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024151.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050525 - cutinase activity [Evidence
                     IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ethylene terephthalate) hydrolase family
                     protein"
                     /protein_id="HIR1404876.1"
                     /translation="MEKVKKSAKKWLCISIALMLLSAIVVSFIQTDGGKVTMKDLKIE
                     TDDGYAMSCYLFVPDSATAETPAPAVVTSHGYLNNKEMQDANFVELARRGYVVLAIDQ
                     PMHGNSDNYSSSSANGVYQGALALSRMPFVDTARIGVTGHSMGGGSCNSAVAQDNKNE
                     TRIISAVLLNCADATYKDDIDGYADVYGSRDVGILAAQYDEFFHKFTDDGGVAREAPY
                     FLTGRNAQSFLNFGADPDGLETRQAYQVYKQQVDGEEAIRVIYNPGIIHPWSHFSAQS
                     TASTIEFFEEAFGAPNPLASSSQVWQFKEAFNFVGVIGFVLFLVSFATLMLYTPAFAS
                     LRAETVVQPAEVTDKKGKIWFWGSLAAGALFSMLIYRWILKLGTSMPVNQTESMGIGL
                     WACLCGLFTILSMVVFYFAYGKKHGLDLAERGVKLPLKKLGKTALLAVIVAAVCYGWV
                     FAADYFFKADFRLWTLAIKAFEADKLGNALTYIWLFLVFYVAASVSVNCFNYNTACGK
                     KHPWVNGLVVALFAALPAIVLPAAQYITYFSSKAMLWPASAMHVLWLFPVILILFASA
                     LISRCLYKVTKNPYLAGIISAIIITLITVTNTCTTL"
     gene            complement(4546..5121)
                     /locus_tag="ACZPFK_00810"
     CDS             complement(4546..5121)
                     /locus_tag="ACZPFK_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1404877.1"
                     /translation="MELEWLGKSRPLIEDLFYWSNTYAASCKKKVALTEKIALSASEI
                     QVIEYLLENEELQLNMAGVARRLGISPVSFTNLVSRLEKLGLVQKYYREGDRKSMIVL
                     VTALGREIYGISIPASCASCGKKTCCRRWTRSPSPIRKSFARCCAASTSPTAFLQRRR
                     RPSIRSRTAAEAAPAPCGPQGGNLDAVVFVL"
     gene            complement(5779..6696)
                     /locus_tag="ACZPFK_00815"
     CDS             complement(5779..6696)
                     /locus_tag="ACZPFK_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR1404878.1"
                     /translation="MNKITEDLLKIVSDWKGIPDAAYNIREDTGCAGRRSTENIRIDS
                     KTDKPGIDIHVKPGTRGEIVYIPACVTHSAVDDLVYNDFYIGAGADVTIVAGCGVHSD
                     GEEEAKHNGVHRFFLEKGARVLYLEKHIGTGGGAGARVINPVTDAELAEDAYLEMNTT
                     QIGGVDSTLRKTTAVLGKNARMIVKEKLLTEGAQTAESDFVITLAGEGSGAHLISRSV
                     AKQHSSQLFRSRLVGEAACSGHSECDAIIMDDGVVSAIPELTAHNVDAALIHEAAIGK
                     IAGEQITKLMTLGLTEKQAEEKIIEGFLK"
     gene            complement(6693..7436)
                     /locus_tag="ACZPFK_00820"
     CDS             complement(6693..7436)
                     /locus_tag="ACZPFK_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1404879.1"
                     /translation="MLELKSISFTVSSAAEQAEKGILDDVSLTVGDNQFVVITGPNGG
                     GKSTLAKIIMGIERPTAGRILFDGQDITDLSVTQRAELGIAYAFQQPVRFKGLTVSDL
                     VGLAARKELGRGELCQYLTEVGLCARDYIDRDVNASLSGGEIKRIEIAMALARGAKLT
                     LFDEPEAGIDLWSFNHLIAVFQKLRRETRGTSLIISHQERILDIADEIVVLAAGRIAA
                     RGHKDEVLPKLLETGSSVNFCEGKVEANA"
     gene            complement(7633..7863)
                     /locus_tag="ACZPFK_00825"
     CDS             complement(7633..7863)
                     /locus_tag="ACZPFK_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404880.1"
                     /translation="MDTKYPERYRIMGFKIAYYRKKSGYTQEAFAEKIGKSVNFLAQV
                     EGPGTIKGISLETLFTMAEVLKIPPSKLLEDD"
     gene            7999..8664
                     /locus_tag="ACZPFK_00830"
     CDS             7999..8664
                     /locus_tag="ACZPFK_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404881.1"
                     /translation="MAWEKDVEPIQQKNSLWPFRVSPPKEEIEMENQLMSWRLIQING
                     HERQSIYESRQTRGKRAALLFGPGLCRGVMQESLLIEMAVAFLTHVWEVARAQPGSCL
                     ARGLYHLQQLERSKGRFFAPLRDETFCSAYITMMETQRALLWLFLEEEKYDREMQKNL
                     RQEELECASDVSPTLLKALGHLDLKEVESLSARSAEQLRQDIEVCSLCRGLWELWEKN
                     SLV"
     gene            complement(8860..>9471)
                     /locus_tag="ACZPFK_00835"
     CDS             complement(8860..>9471)
                     /locus_tag="ACZPFK_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1404882.1"
                     /translation="SYGVERPLSAIENLSATTLRNIIGDMELDHTLTSRDIINGKITT
                     ILDEATDKWGIKVNRVEVKNIIPPAEIQDAMEKQMKAERQRRESILRAEGEKRSAILV
                     AEGEKESAILRADAVREQRIREAEGEAQAMMTVQKALADSIRLLNDADPTQKVITLKS
                     LEALQKVADGKATKLIIPSELQNLGGLVSSIKEMATDVKPESK"
BASE COUNT         1731 a         2779 c         3005 g         1956 t
ORIGIN      
        1 ccgtccgccc cacggccacg gcgaccggcc gccgactggc cccgcgcgga cggcgtcaaa
       61 aatatttttc cgtcaataca gcggcgaggg cccctccccc gccggaacgc tggtgatttt
      121 ttggacaaat acaaacggct ggtgtcgaac acggtgctct ttgcgatcag ctcgttttcg
      181 agcaagatcc tctcctttct gctcatgccg tttttcaccc ggatgttcgg cctgggcgag
      241 ttcggcgacg cggacctgat caccaagtac gcccagctgc tgctgcccat cgtctcggtg
      301 ggcatcgcca acgcggtgat ccgctacggc ctggacaaga cctacaacaa ggcctcggtg
      361 ttcaccggcg gcctcatcgc cgagggggtg ggcatcgccg tcttcgcggc ctgctggccg
      421 ctggtggcgc gcatcccgga catgggcggc tacaccgcgc tgctctatgc ctatgtggtg
      481 atgagcattc tgcgcaacct gtgcagccag tttgtgcggg ccaagcagta cacccggctc
      541 tacgcggtgg acggcgtgct caacaccgtg ctctacctgt tttttatcgt gctcttcatc
      601 gcgggcttca agtggggcat cgcgggctat gtgctggcca ccgcggcggc ggatttctgc
      661 tccgcgctgt tcctcttttt caccgcgcgg ctctaccgct acatccgcat ctcccgcttc
      721 gaccggccgc tgctgcggga gatgctgcgc tacgccgcgc cgctgatccc caccagcatg
      781 ttctggtgga tcaccaacac cagcgaccac atgttcatca cctccatgct gggcagcgag
      841 gccaacggcc tgtatgtggc ggcctacaag gtgcccaacg tgatcatgct gttcagcacc
      901 ctgttcaccg aggcctggca gctctcggcc gtgaccgacg gcggccgcaa ttcgcccggc
      961 cgcgccaaat ttttcagcca ggtgttcctc agctaccaga gcctgctctt tatcgtggcg
     1021 ggcgggctga tctattgctg caaatttgtg atgatggtgc tcaccctctc gggcaagtcg
     1081 gcgttcaacg cggcctggca gtatatcccg ctgctggtcg tggccacggt cttcagctgc
     1141 tttgtcacct ttttgggcag cgtctatatg gtggaaaagc gctccggcga gagctttttc
     1201 accatgctgg tgggcgcggt gctcaacctg gcgctcaact ggttcctgat cccggtctgg
     1261 ggccccaacg gggccgcgtt cgccaccttt gccagctatc tgctggtttt cgtcatccgg
     1321 gcgttcagca cccggcgcta tatcgccatg gacctgcacc tgggccgcat gacggtcacc
     1381 ctggccctgc tggccgggga gatcgccctc atgctgtccg aggcgaaata ctggcagctc
     1441 tggtgcgggc tgctctgcgc cgcggtggtg gcgttcaatt tctggcccat tttgcagacg
     1501 gtgcggcggc tgctggcccg ccgcagaaga agaagctagc gggccggggc ggccccgcaa
     1561 agaggaggct gcgatgagag ttttattcgt gggcaacagc cacacctatt tcaacgatat
     1621 gccccacacc tttgcccggc tgtgcgccgc cgcgggcgcg gggcagccgc agaccgtgct
     1681 gctggcccac tcggcccggc cgctgggctg gcatctggag gagtattttg agctgcggta
     1741 caacctgctg tacggcggct acgactactg cgtgatccag cagcaggcgc accctttccc
     1801 cggtgaggag gagaccgcgc gggactgcgg gcgcatcatc ggcctctgcc gcgccgcggg
     1861 cgcccgcccg gtgctgctga tgacctgggc cgagcgcgcc gcgccggagc accagcaggc
     1921 catgacccgg gcctaccgcg ggctggcggc ctccaccggc gccctgctgg ccccggtggg
     1981 ggagatctgg cagcagttga gacgggagcg cccggagatc gagctgttct ggcgcgacgg
     2041 cgagcacgcc tcgccgctgg gggattatct ggtggcggtt accctctgcg cggtgctcac
     2101 cggggccgac ccggaggcgc tgcccggcct gggggcggac tttgccgggg aggccgaaat
     2161 cgcctttttc gaccacccgc gggtgcggga ggacccggcc tgcattgaga ccccgctgga
     2221 cccggccgcc tgcgcggcga tccgcagggc ggtgaagcgg gcctgcgcgg caaaggcgcc
     2281 ctgagcgggg cgcgccggcg cggaccgaaa agaaagaaaa agcgatctcc cgccgtcagc
     2341 agcgggagac cgcttttttg tccccgggcc ggagagccgg ccccgggcga tgcgaaaaaa
     2401 ggccggcccc ggcggggaga ggtcccgccg gggccggtat ggggaatgga tcacagcgtg
     2461 gtgcaggtat tggtcactgt gatgagggtg atgatgatgg cgctgatgat gccggccaga
     2521 taggggttct tggtgacctt gtagaggcag cgggagatca gcgcggaggc gaacaggatg
     2581 aggatgaccg ggaacagcca cagcacgtgc atggcgctgg cgggccagag catcgctttg
     2641 gaggagaagt aggtgatgta ctgggcggcg ggcagcacga tggcgggcag ggctgcgaac
     2701 agcgccacca ccaggccgtt cacccaggga tgctttttgc cgcaggcggt gttgtagttg
     2761 aagcagttga ccgagacgga ggccgccacg taaaagacca ggaagagcca gatgtaggtg
     2821 agggcgttgc caagcttatc cgcctcgaac gccttgatgg ccagggtcca cagccggaag
     2881 tccgccttga agaagtagtc ggccgcaaag acccagccat agcagaccgc cgcgacgatg
     2941 accgccagca gagcggtctt gcccagcttt ttgaggggca gcttcacgcc gcgctccgcc
     3001 agatccaggc cgtgcttttt gccgtaggcg aagtagaaga ccaccatgct gaggatggtg
     3061 aacaggccgc acaggcaggc ccaaaggccg atgcccatgg attcggtctg gttgacgggc
     3121 atcgaggtgc ccagcttgag gatccagcgg tagatcagca ttgagaacag cgcgcccgcg
     3181 gcgagcgagc cccagaacca gatcttgccc tttttgtcgg tgacctcggc gggctggacc
     3241 accgtctcgg cccgcaggct ggcgaaagcg ggggtgtaga gcatcagggt ggcgaagctg
     3301 accaaaaaga gtacaaagcc gatcacgcct acaaagttga acgcctcttt gaactgccac
     3361 acctggctgg aggacgcgag gggattgggc gcgccgaaag cctcctcaaa gaactcgatg
     3421 gtggaggcgg tggactgggc gctgaaatgc gaccagggat ggatgatgcc ggggttgtag
     3481 atgacgcgga tggcctcttc gccgtccacc tgctgcttgt agacctgata ggcctggcgg
     3541 gtctcaaggc cgtccgggtc ggcgccgaag ttcaggaaag actgggcgtt gcgcccggtg
     3601 aggaagtagg gcgcctcccg cgcgacgccg ccgtcgtcgg tgaatttgtg gaagaactcg
     3661 tcgtactggg ccgccaggat gcccacgtcg cgggagccgt aaacgtcggc gtagccgtcg
     3721 atgtcgtctt tgtaggtggc gtccgcacag ttgagcagca cagcggagat gatgcgggtc
     3781 tcgtttttat tgtcctgcgc cacggccgag ttgcaggagc cgccgcccat cgagtggccg
     3841 gtgacgccga tgcgcgcggt gtcgacgaac ggcatgcggg acagggccag cgcgccctga
     3901 tagaccccgt tggcgctgga cgacgagtag ttgtcggagt tgccgtgcat gggctggtcg
     3961 atggccagca ccacatagcc gcggcgggcc agctccacaa agttggcgtc ctgcatctcc
     4021 ttgttgttca ggtagccgtg gctcgtgacc actgccggcg ccggggtctc ggcggtggcg
     4081 ctgtcgggga cgaacagata gcaggacatg gcgtagccgt cgtcggtctc gatcttcagg
     4141 tctttcatgg tcaccttgcc gccgtccgtc tggatgaacg acacgacgat ggcgctgagc
     4201 agcatgaggg cgatagagat acagagccat ttttttgccg attttttgac cttttccatg
     4261 gtttctcctc caatagtttt attcttgcac cgcagacccc tgcagcgttc aaggactggt
     4321 gaaaaggacg gccgcccgcc ggcggcggga gcaaaaaaga cgcacaacgt gaagcaacac
     4381 ttcacatcgc gcgtctctca ttctcttcgc gtttctgttt tgctggtctc attctaacag
     4441 actctgcctc aaagaatcaa tatgaattat tcatgaataa aatgattaaa cattgtacaa
     4501 tatagctttt gtttttaata aaattattaa tttgatgaac gattttcaca aaacaaaaac
     4561 aaccgcgtcc agattccccc cctgcgggcc acagggggcg ggtgcggctt cagcggctgt
     4621 ccttgaccgg atggagggcc ggcgcctcct ttggagaaaa gctgtcggcg acgtagaggc
     4681 tgcgcagcac ctggcgaaac tttttctgat agggctcggg gatctcgtcc agcgcctgca
     4741 gcacgtcttt ttcccacagc tggcgcagct tgcgggaata gagatgccgt agatctcgcg
     4801 cccgagcgcg gtcaccagca cgatcatgct cttgcggtcg ccctcgcggt aatatttctg
     4861 caccaggccc agcttttcca gccgggagac aaggttggtg aacgagaccg gggagatgcc
     4921 cagcctgcgc gccacaccgg ccatgttgag ctgcagctcc tcgttttcca gcaggtattc
     4981 gatcacctgg atctcgctgg ccgagagggc gatcttctcg gtcagggcga cctttttttt
     5041 gcaggaggcg gcataggtgt tgctccagta aaaaaggtct tcgatcaatg ggcggctttt
     5101 gccgagccat tccagttcca taaaaacacc tctggtaaat tgtgaagtac ccgtccccag
     5161 cggggcgggg cagcggttcc cgggatgatg aactttatta tagcatattt tccgcggttt
     5221 tgaaaaacca gccggcggaa agggacgttt ttccacaaaa cagctgcggc cccttgtaaa
     5281 gcggaggtgc gggtgcggcg cgtagcctct gccgccccct accctgccgt cgagccgaca
     5341 aagggggaaa taaaaaaaca gccgcagcaa agcggaggct attttttcgg ccatgtggcc
     5401 catccctggg ccacggccat tagaatcagt tgttttttat tgcggagcaa taaaaaacag
     5461 ccgcagcaag gcgcaggcta ttttttcggc catgtggccc atccctgggc cacggccatt
     5521 aaaatcagtt gttttttatt gcggagcaat aaaaaacagc cgcagcaagg cgcaggctgt
     5581 tttttcggcc atgtggcccg tcctcgggcc acggccatca gaatcagttg ttttttatgg
     5641 cggagcaata aaaaaaggcg cggcccggcg actgcggccg gttcgcgcct ttctgcatgc
     5701 tttgagctgt gccctgcccc ctggggcaaa ccaaagcccg ccggggacag aggcaaaggc
     5761 ccggcggcgg cgaaaagcct atttcaaaaa gccctcgatg atcttttcct ccgcctgctt
     5821 ctcggtcagg cccagggtca tcagcttggt gatctgctcg cccgcgatct tgccgatggc
     5881 cgcctcgtgg atcagcgccg cgtccacgtt gtgggcggtc agctcgggga tggcgctcac
     5941 caccccgtcg tccatgatga tggcgtcgca ctccgagtgg ccgctgcagg ccgcctcgcc
     6001 caccaggcgg gagcggaaga gctgcgacga gtgctgcttt gccaccgagc gggagatcag
     6061 gtgcgcgccg ctgccctcgc cggccagcgt gatgacgaaa tcgctctccg cggtctgcgc
     6121 gccctcggtg agcagcttct ctttcacgat catcctggcg tttttgccca gcacggcggt
     6181 ggtcttgcgc agcgtggagt ccacgccgcc gatctgggtg gtgttcatct ccagataggc
     6241 gtcctcggcc agctcggcgt cggtgacggg gttaatcacc cgcgcacccg cgccgccgcc
     6301 ggtgccgatg tgcttttcca gatacagcac ccgggcccct ttctccagaa aaaagcggtg
     6361 tacgccgttg tgcttggcct cctcctcgcc gtccgagtgg acgccgcagc ccgccacgat
     6421 ggtcacgtcg gcgcccgcgc cgatataaaa gtcgttgtag accagatcgt ccacggcgct
     6481 gtgggtgacg caggcgggga tatagacgat ctcgccgcgg gtgccgggct tgacatggat
     6541 gtcgatgccc ggcttgtcgg tcttggaatc gatgcggatg ttctcggtgg agcggcggcc
     6601 ggcgcagccg gtgtcctcac ggatgttata ggccgcgtcg gggatgcctt tccagtccga
     6661 gacgatcttg agcaggtcct cggtgatctt gttcatgcgt tcgcctccac tttgccctcg
     6721 cagaaattga cgctgctgcc ggtctccagc agcttgggca gcacctcgtc cttgtggccc
     6781 cgcgccgcga tgcggccggc cgccaacacc acgatctcgt ccgcgatgtc caggatgcgc
     6841 tcctggtggg agatgatgag cgaggtgccc cgggtctcgc ggcgcagctt ttgaaacacc
     6901 gcgatcaaat ggttgaagct ccacaggtcg atgcccgcct cgggctcgtc gaagagggtc
     6961 agcttggcgc cgcgggcaag ggccatggcg atctcgatgc gcttgatctc gccgcccgaa
     7021 aggctggcgt tcacgtcgcg gtcgatatag tcgcgggcgc acaggcccac ctcagtcaga
     7081 tattggcaca gctcgccccg gcccagctct ttgcgggcgg caaggcccac caggtcgctg
     7141 accgtcaggc ccttgaagcg caccggctgc tgaaaggcgt aggcgatgcc cagctcggcg
     7201 cgctgggtga ccgacagatc ggtgatgtcc tgcccgtcga acaggatgcg cccggcggtg
     7261 gggcgctcga tgcccataat gatcttggcc agcgtggact tgcccccgcc gttggggccg
     7321 gtgatgacga cgaactggtt gtcgcccacg gtgaggctga cgtcgtcgag gatgcccttt
     7381 tcggcctgct cggcggcgct ggaaacggta aaggaaatag acttgagttc cagcattctg
     7441 gtacctccgc aaagatgttc cgccgcgcac aggggcgcgg cgcgggcgcg gcaaggtctg
     7501 cgcccaaacg ccctaggata ttatagcaca caaaacagcc ccgtacagcc ctttttcaaa
     7561 aaatcataca aaaaaagtat agattagggc taaaaataaa aagccctctg cgcctttatc
     7621 gcaaagggtt tgctagtcgt cttccaacaa tttggaggga ggaattttta agacttcagc
     7681 catggtaaaa agagtttcca gagagatgcc ctttatggtg ccgggtccct cgacctgagc
     7741 caaaaaatta acgctttttc cgatcttttc cgcaaaagcc tcttgtgtgt acccggattt
     7801 cttgcggtaa taagcgatct tgaaccccat gatccgatag cgctcaggat actttgtgtc
     7861 cacatcctca cttccttttt tatatgctat cagtgataga cataaattat aatttcttta
     7921 aaggaaatga ttatttactt ttagtaaatg acacgataaa ataaaataaa gtctaaccgg
     7981 atcggggaga gggaaaatat ggcttgggaa aaagacgttg agcccataca gcaaaaaaat
     8041 tctttatggc cgtttcgtgt ttcgccgccc aaagaggaga tcgagatgga aaaccagctg
     8101 atgtcgtgga ggctgatcca gataaatggt cacgaacggc aatctattta tgagagccgg
     8161 cagacgcgcg gaaaacgtgc ggccttgctt tttggtccgg gcctttgtcg gggcgtgatg
     8221 caagagtcgc tgttgatcga aatggcggtg gcttttctaa cccatgtatg ggaggtggcg
     8281 agggcgcagc ccgggtcttg tttggcgcgg ggcctctatc atctccaaca gctggagcgg
     8341 tcaaagggca gattttttgc tcctttgagg gatgagacgt tctgcagtgc ttatatcacg
     8401 atgatggaaa cgcagcgggc actgctatgg ctttttctgg aggaagaaaa gtatgacagg
     8461 gagatgcaaa aaaacttgcg gcaagaagaa ctggagtgcg cctccgatgt gtcccccacc
     8521 cttttgaaag ccctggggca ccttgacctt aaagaggtgg aatcactttc tgcccgctcg
     8581 gcagaacagt tgagacagga cattgaagtc tgttcccttt gccgtggttt gtgggagctg
     8641 tgggagaaaa attcccttgt ttgaagattg cggcactttc tgcacccgag aaaagtttga
     8701 gggaacgccc cctccgcccc ctctcggcgg cagctcccct gaacggggag ccgtgcggcg
     8761 cctgcccttg gcgtttcgaa aggctgggaa gcggcgcagt ggaaaacaca aaaaagcagc
     8821 gccgggtctt cggcgctgct tttgcggttt gggtgaaagt tatttggact ccggcttcac
     8881 gtcggtggcc atctctttga tgctggacac caggccgccc aggttctgca gctcgctggg
     8941 gataatgagc ttggtcgcct tgccgtcggc cactttctgc agagcctcca ggctcttcag
     9001 ggtgatcacc ttctgggtgg ggtcggcgtc gttcagcagc cggatggaat cggccagggc
     9061 tttctgcacg gtcatcatgg cctgggcctc gccctcggcc tcgcggatgc gctgctcgcg
     9121 cacggcgtcg gcgcgcagga tggcgctctc cttttcgccc tcggcgacca ggatggcgct
     9181 gcgcttttcg ccctcggcgc gcaggatgct ctcacggcgc tggcgctcgg ctttcatctg
     9241 cttttccatc gcgtcctgga tctcggcggg cgggatgatg ttcttgacct cgacccggtt
     9301 gaccttgatg ccccacttgt cggtggcctc gtccaggatg gtggtgatct tgccgttgat
     9361 gatgtcgcgg ctggtcaggg tgtggtcgag ctccatgtcg ccgatgatgt tgcgcagggt
     9421 ggtggcggac aggttctcga tggcggacag cgggcgctca acgccgtagc t
//
LOCUS       DBMDYA010000016         9432 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_1988_length_9432_cov_1.408745, whole genome shotgun sequence.
ACCESSION   DBMDYA010000016 DBMDYA010000000
VERSION     DBMDYA010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_9432_cov_1.408745"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="ACZPFK_00840"
     CDS             complement(<1..643)
                     /locus_tag="ACZPFK_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1404883.1"
                     /translation="MANILVVDDEPDLRALLQAALQKDGHQVTALGRGDEVTPRHCAW
                     ADCILLDVMMPGEDGFAACARIRGEADCPILFLTAKGEEQDVLRGLALGGDDYLTKPF
                     RLAELRARVAAHLRRQGRAPTHRLRRGEYEFDLAGREMSFSGRPVKLTRGEYAICEHL
                     AAHAGQTLGKEQIYEAVFGYEGEADASAVTEHIKNIRAKCRAAGAQELPVETVW"
     gene            complement(674..1534)
                     /locus_tag="ACZPFK_00845"
     CDS             complement(674..1534)
                     /locus_tag="ACZPFK_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1404884.1"
                     /translation="MELERGTAHMTAKRRAGLTGFDLKIIALALMVLDHIHYFFEFTG
                     AVPLLFTQLGRLSAGLFLFSVVEGFVHTHDRKKYFLRIYLISLAMGLALYGMLVFCIA
                     RPDGMIPMNQIMANFVILLPMLQGCEWLRQRRWGRGLAALLLPMAWPYLVIYLLCPLF
                     PALNGWVALVGLTVLPMHTILMDGGTWYVLSGLLLYLLRKNRFLQAGGYFIFNLLVYG
                     GMALALPGITAAAFFGTYFEWMCAFSAVFMVFYNGERGKNIKSFFYVFYPAHIYVLFG
                     LSCLVWAWLH"
     gene            1841..2395
                     /gene="dhaS"
                     /locus_tag="ACZPFK_00850"
     CDS             1841..2395
                     /gene="dhaS"
                     /locus_tag="ACZPFK_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HIR1404885.1"
                     /translation="MADSALTKRVLADALKELMQTRPLAKISVGDITAHCGINRQTFY
                     YHFKDKYDLVGWIYSTETLPFMGSFSDRAHWTDGLRDLCLYVQQNKKFYVNALNTPGQ
                     NSFEEYLTQFLQSLIDALIEEESGGRIAKEDRTFIADFYAFAFVGLVMKWARGGMKED
                     PAYYVGRIRELIDGSLLRELEKYK"
     assembly_gap    2420..2429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2865..3239
                     /locus_tag="ACZPFK_00855"
     CDS             2865..3239
                     /locus_tag="ACZPFK_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1404886.1"
                     /translation="MEIIISNSSGKPIYEQITSQIKNGILTGALSEGEALPSMRQLAK
                     DLRISVITTKRAYEDLERDGFLTNVPGKGCFVAVKNPEFLREESLRMVEEALQRAVDA
                     AKSGGVTLAELREALNLLYEGE"
     gene            3243..4109
                     /locus_tag="ACZPFK_00860"
     CDS             3243..4109
                     /locus_tag="ACZPFK_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404887.1"
                     /translation="MKNAIEVRHLSKQYSGFALQDVSFTVPCGSIMGFVGENGAGKTT
                     TIKAILGLIRPDGGETELLGRPMSDAEREVKSQIGVVFDETYFHDNLTLRVIDKMLGH
                     IYKGQWDSGAFAAFCSRFNLPQGKEVKAFSRGMKMKLSIAAALAHHPKLLILDEATSG
                     LDPIVRDEILDVFYDFIQDEEHSILFSSHITSDLEKVADYITFIHEGRIVFSKNKDDL
                     VYSYGVARCTAQQFDRIDADEYLRRRRSAMGGEELLVADRESFARRHPSVVLDNAGVE
                     DIMLFYVKGERA"
     gene            4205..4828
                     /locus_tag="ACZPFK_00865"
     CDS             4205..4828
                     /locus_tag="ACZPFK_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1404888.1"
                     /translation="MSGLIYKDLMNLRRYLKQLLLIFVVFVFIFGFSGGNYSFASAYG
                     VVLSAMVVLSSMSYDEQAKWDKYALTMPLSRRLLVGAKYLLSLLAIGVGALLSLAVVV
                     ISALLGKAPMMEGLATVGACACVALLMLAVLLPCLYKFGIEKARMMMIVIFLLPTLAI
                     LALSKLNVPMPGEEFWAGLLIAIPFVAALGYLLSYLVSVSIFSKKEL"
     gene            4836..5057
                     /locus_tag="ACZPFK_00870"
     CDS             4836..5057
                     /locus_tag="ACZPFK_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404889.1"
                     /translation="MKQKGVCPKCGSRQLLRVPDHPGRYASGNNIYTTTFTLAGKVPV
                     IRYVCLDCGYVENWVDNAADLAKLRRAFG"
     gene            5079..5258
                     /locus_tag="ACZPFK_00875"
     CDS             5079..5258
                     /locus_tag="ACZPFK_00875"
                     /inference="COORDINATES: protein motif:HMM:NF039785.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HIR1404890.1"
                     /translation="MDTKEQRFVETEAQSGLRGVSVLVDRQTGVQYLFVKAGNAGGLT
                     PLLGRDGRPLLAPAP"
     gene            complement(5352..5948)
                     /locus_tag="ACZPFK_00880"
     CDS             complement(5352..5948)
                     /locus_tag="ACZPFK_00880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR1404891.1"
                     /translation="MEKEFLGGYTSGWWQAARRNRALVRRYNTMDPELLWEKYQLLKE
                     LLGEVEEHALIEPPFYCDNGKNIRVGKNFYANYNLVILDADKVTMGDRVVIGPNVTFC
                     AASHPVHPQSRFHGGAYPVLTGPITVGDDVWIGANVTVMMGVTIGSGATIGAGSVVTR
                     DIPAGVVAAGAPCRVLRQVTDADRYDWPAEEAAPDTGL"
     gene            complement(6173..7633)
                     /gene="pepD"
                     /locus_tag="ACZPFK_00885"
     CDS             complement(6173..7633)
                     /gene="pepD"
                     /locus_tag="ACZPFK_00885"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HIR1404892.1"
                     /translation="MDYVTKGLEPADALRYFEELSRIPRGSRNEAAVAQYIYDLAKGL
                     GLEAYKDEANNVVVKKPASPGCEDKPPLMLQGHTDMVCVKLPDCGHDFEKDPLKLEIR
                     DGQLTAVGTTLGADDGTACAHMMALMARDDYVRPPLEFVFTSMEEIGLLGAKALDPAL
                     IKARRMVNMDSGASEDYQTVVSCAGGIVYDFKKKPRWEAADGDAVRLHISGLLGGHSA
                     ITIGEGRGNGLKLMARLLHAVAAAMEMRVAHFEGGMKMNAIVSDATALVTVPAGRGPE
                     ALQIAETLAAAIREELKISDPGFTFEGGAAELPAQVLVEGDGRALVDFLFLLPDGMRF
                     MSREIEGLVHCSSNIGILTMRPDEIYLCDCIRTAEDSLGDQLGGEFETLAGLLGFEAV
                     RTGGFCGWKYDPASPLRACGMALYKKMFGREMEIQATHGGLECGEFKGKFPDMDIMTV
                     APTCAGAHTTEETMDLASFKRVLDYLLALFEELCKE"
     gene            complement(7833..8975)
                     /gene="metK"
                     /locus_tag="ACZPFK_00890"
     CDS             complement(7833..8975)
                     /gene="metK"
                     /locus_tag="ACZPFK_00890"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1404893.1"
                     /translation="MSKRFFTSESVTEGHPDKICDQISDAILDAILAEDPDGRVACET
                     AVSTGLVHVMGEITTSCYVDIPRLVRDTVREIGYTRAKFGFDADTCAVITNIDEQSPD
                     IAMGTNDQVGGAGDQGMMFGFACDETPELMPLPISLAHRLAKRLSEVRRDGTLDYLRP
                     DGKTQVTVEYDDGRPVRVDTVVVSAQHAPEVPIEQLRAEIIEQVVKPIVPAGLLDEGT
                     SYLINPTGRFVVGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSGAYAA
                     RWVAKNIVAAGLASKCELELAYAIGVAQPVSVLVDTFGTGKVEDDRIAEAVKKVFDLS
                     PAGIIRTLDLRRPQYKALAAFGHMGREDLGVTWERTDRVEELKAAL"
     regulatory      complement(9161..9261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1821 a         3043 c         2782 g         1776 t
ORIGIN      
        1 cccataccgt ctccaccggc agctcctgcg cgccggcggc gcggcacttt gcccggatgt
       61 ttttgatgtg ctcggtcacg gctgaggcgt ccgcctcccc ctcatagccg aacaccgcct
      121 catagatctg ttccttgccc agcgtttgcc ctgcgtgggc ggcaagatgc tcgcagatgg
      181 catactcccc acgggtgagc ttgaccggtc tgccggaaaa cgacatctcc cggcccgcga
      241 ggtcaaactc atattctccc cgccgcagcc gatgggtcgg cgcccgaccc tgccgccgca
      301 ggtgcgccgc aacgcgggcg cgcagctccg ccaggcgaaa gggctttgtc agataatcgt
      361 ccccgcccag cgccaggccc cgcagcacgt cctgctcctc ccccttggcg gtgaggaaca
      421 aaatcgggca gtccgcctcg ccgcggatgc gggcgcaggc ggcaaagccg tcctctccgg
      481 gcatcatcac atccagcagg atgcagtcgg cccaggcgca gtgccggggc gtgacctcgt
      541 cgccgcgccc cagcgccgtc acctggtgcc cgtccttttg cagcgccgcc tgcagcagcg
      601 cgcgcaggtc gggctcgtcg tccaccacta aaatattcgc catcccgttc gcctccctct
      661 gagccgcccc tggtcagtgc agccaagccc acacaaggca ggaaaggccg aacagcacat
      721 agatgtgggc gggataaaaa acatagaaaa agctcttgat gtttttgccc cgttccccgt
      781 tatagaatac cataaacaca gccgaaaagg cgcacatcca ctcaaaatag gtgccaaaaa
      841 aggccgccgc cgtgatgccc ggcaacgcca gcgccatgcc gccgtagacc agcagattga
      901 agataaaata gccgccggcc tgcaaaaatc gatttttgcg caacagatat aaaagcagac
      961 ccgagagcac gtaccaggtg cccccgtcca tgaggatggt gtgcatcggc agcacggtga
     1021 ggcccaccag cgccacccag ccgttcagcg ccgggaacag cgggcacagc agatagatga
     1081 cgagataggg ccaggccatg ggcagcagca gggccgccag cccgcggccc cagcggcgct
     1141 gccgcagcca ctcacagccc tgcagcatgg gcagcaggat gacgaagttt gccatgatct
     1201 ggttcatggg gatcatgccg tccggccgcg cgatgcaaaa gaccagcatc ccgtacaatg
     1261 ccagccccat ggcgagcgag atcagataaa tgcgcagaaa atattttttg cggtcgtggg
     1321 tgtggacaaa gccctccacc accgaaaaga gaaaaagccc cgctgagagc ctgcccagct
     1381 gagtgaaaag cagcggtacc gcaccggtga attcgaaaaa atagtgaata tggtccagca
     1441 ccatcaacgc caaggcgatg atcttgagat caaagccggt cagccccgca cggcgttttg
     1501 ccgtcatgtg cgccgtacct ctctccagtt ccataaaaaa tcctcccttt tgcaattggt
     1561 ctgctttcag cataagggag atttctcagg aaatgatcag ggagacaaat ttttttgggc
     1621 ccgccgccgc gcgggcgact tttcttgaaa aaagtgccaa aatcctttag aatggagatg
     1681 ttcggttgac acggacgcct tagggtttta tccccgcgcc ggtgcgccgc tgaacacagc
     1741 gggccccaaa gcacagcttc cctcccccgg ccgcgggggc cggctgagcg gcggaagagc
     1801 ccgcgggtat tgattttctg gcaagcaaag gaggacgagc atggcggatt cagccctcac
     1861 caaacgggtg ctggcggacg cgctcaagga gctgatgcag acccggcccc tggccaagat
     1921 ctcggtgggc gatatcaccg cccactgcgg catcaaccgc cagacctttt attaccattt
     1981 taaggacaag tacgatctgg tggggtggat ctattccacc gagaccctgc cctttatggg
     2041 cagtttttcc gaccgtgccc actggacgga cgggctgcgc gacctgtgcc tctatgtgca
     2101 gcagaacaaa aaattttacg tcaacgccct gaacacgccc ggccagaaca gctttgagga
     2161 atatctcacc cagttcttgc agagcctgat cgacgccctg atcgaagagg agagcggcgg
     2221 ccgcatcgcc aaggaggacc gcacctttat cgccgacttt tacgctttcg cctttgtggg
     2281 cctggtgatg aaatgggcgc gcggcggcat gaaagaggac ccggcctatt atgtcggccg
     2341 catccgcgaa ttgatcgacg gcagcctatt gcgggagctg gaaaagtaca aatgacgcgc
     2401 ccgcctccgc gcggcgcaan nnnnnnnnnt ttatagcttt gcaacgcaaa gctgtaaaaa
     2461 cggccatgtg ggccgtcccg gcccacggcc tcaaaatcta gcggcccgcc cacgggagtg
     2521 ggcggatgcg cgtaaaaaat agccgcaggc tattttttat tgctgcgcac taaaaaacaa
     2581 cttatctcga tggccgtggc ccaaggatgg gccacatagc cgaaaagcgt ccgccgcaaa
     2641 aaaaccggac agacaagtcg ccttgcgctg caaaaagaga gcgggccgaa aaaacaaatg
     2701 ggcggccgcc gccggtcctc tctcccctgt tttacacccg gaaagcagag cggaaaaaat
     2761 atcctgccaa ctttttgttt ttcctattga caacctttta tcaaggcgtt atactgtgca
     2821 tacagtatat atacaatatt catttccgga aatgaggcag aagattggag atcatcatca
     2881 gcaattccag tggaaagccc atctacgaac agatcacctc gcagatcaaa aacggcatcc
     2941 tgaccggcgc gctgtcggag ggcgaagccc tgcccagcat gcgtcagctg gcaaaggacc
     3001 tgcgcatcag cgtcatcacg accaagcgcg cctacgagga tctggagcgg gacggttttc
     3061 tcaccaacgt gccgggcaag ggctgctttg ttgccgtcaa aaacccggaa tttttgcggg
     3121 aggagtccct gcgcatggtg gaggaggccc tgcagcgcgc ggtggacgca gccaaaagcg
     3181 gcggcgtcac cctggcggag ctgcgggagg ccctcaacct gctctatgaa ggagaataaa
     3241 cgatgaaaaa tgcgatcgag gtgcgccatc tctctaaaca gtattccggc tttgccctgc
     3301 aggacgtgag cttcaccgtg ccctgcggca gcatcatggg ctttgtgggc gaaaacggtg
     3361 ccggcaaaac caccaccatc aaggccattc tggggctgat ccgcccggat ggcggcgaga
     3421 ccgagctttt gggccgcccg atgtcggatg cggagcggga ggtcaaaagc cagattggcg
     3481 tggtctttga cgagacctat tttcacgata atctgacgct tcgggtcatt gataaaatgc
     3541 tgggccacat ctataaaggg cagtgggaca gcggcgcctt tgcggctttc tgcagccggt
     3601 tcaacctgcc gcagggcaaa gaggtgaaag cgttctcccg cggcatgaag atgaagctct
     3661 ccatcgccgc ggcgctggcc catcacccca agctgctgat cctggacgag gccaccagcg
     3721 gcctggaccc catcgtgcgg gacgagatcc tggacgtctt ttacgatttc atccaggacg
     3781 aggagcacag catcctcttt tcctcccaca tcaccagcga tctggaaaag gtcgctgact
     3841 acatcacctt tatccatgag gggcggatcg tattctccaa gaacaaggac gacctggtct
     3901 acagctacgg tgtggcgcgc tgcacggccc agcagttcga ccgcatcgac gcggatgaat
     3961 acctgcgccg ccgccgctcg gccatgggtg gcgaagagct gctggtcgcg gatcgggaga
     4021 gctttgcacg ccgccatccc tcggtggtgc tggacaacgc cggcgtcgag gacatcatgc
     4081 tcttttatgt aaagggagag cgggcctgac cccgtcgccc ccgcgcgccc gcccggcccc
     4141 cgccggagcc ggacgcagaa aaaacaggaa cctcacctct taccgtgaaa ggatgatgac
     4201 agaaatgagc ggtctgattt ataaagatct gatgaatctg cggcgctatc tgaaacaact
     4261 gctgctgatc tttgtggtgt tcgtctttat ctttggcttt tccggcggca actactcgtt
     4321 cgcctccgcc tacggcgtgg tgctcagcgc catggtggtg ctcagcagca tgtcctacga
     4381 cgagcaggcc aaatgggata agtacgcgct gaccatgcct ctcagccgcc ggttgctggt
     4441 gggcgcaaaa tacctgctga gcctgctggc cattggggtc ggcgcgctgc tctcgctggc
     4501 ggtggtggtg atcagcgccc tgctgggcaa agcgccgatg atggaggggc tggccacagt
     4561 gggcgcctgc gcgtgcgtgg cgctgctgat gctggccgtg ctgctgcctt gcctgtacaa
     4621 gttcggcatc gaaaaggcgc gcatgatgat gatcgtcatc tttctgctgc ccacgctggc
     4681 gatcctggcc ctctcaaagc tgaacgtgcc catgccgggg gaagaatttt gggcgggcct
     4741 gctcatcgcc ataccctttg tggcggcgct gggctatctg ctctcctatc tcgtctcggt
     4801 ctcgatcttc agcaaaaagg agctgtgatg cagtgatgaa acaaaaagga gtttgcccca
     4861 aatgcggttc ccgccagctg ctgcgcgtcc ccgaccaccc gggccgctat gccagcggca
     4921 acaacatcta caccaccact ttcaccctcg cgggcaaggt gccggtgatc cgctatgtgt
     4981 gcctggattg cggctatgtg gaaaattggg tggacaatgc ggccgacctg gcaaaactgc
     5041 gccgggcctt tggctgagat aaaaaaggag gggctcttat ggatacaaaa gaacagcgct
     5101 ttgtggagac ggaagcccag agcggcctgc gcggcgtgtc ggtgctggtg gaccggcaga
     5161 ccggcgtgca gtacctgttc gtcaaggccg gcaatgccgg gggcctcacc ccgctgctgg
     5221 gccgggacgg caggccgctg ctcgcacccg ccccgtgatt ggacggtaaa atcaaaaaag
     5281 gggccgggag cggagagtga aatctccgct cccggcccct tttttggcgg tcccatccaa
     5341 gcgcctttcc cctacagccc ggtatcgggg gccgcttcct ccgccggcca gtcgtagcga
     5401 tcggcgtcgg tcacctgtcg cagcacccgg cagggcgccc ccgcggccac cacgcccgcc
     5461 gggatgtcgc gggtcaccac gctgccggcg ccgatggtgg cgccgctgcc gatggtcacc
     5521 cccatcatga cggtgacatt ggccccgatc cagacgtcgt cccccaccgt gatggggccg
     5581 gtgagcaccg gataggcgcc cccgtgaaag cggctctgcg ggtgcaccgg gtgagaggcg
     5641 gcgcaaaagg tcacattggg accgatcacc acccggtccc ccatggtcac cttgtccgcg
     5701 tccaggatca ccagattata gttggcataa aagtttttgc ccacccggat atttttgccg
     5761 ttgtcgcagt aaaagggcgg ctcgatgagc gcgtgctcct ccacctcgcc taaaagctct
     5821 ttgagcagct ggtatttttc ccacagcagc tcggggtcca tagtgttgta ccgccgcacc
     5881 agcgcccggt ttctgcgggc cgcctgccac cagccgctgg tgtagccgcc caaaaattct
     5941 ttttccattc ttttttcctc tcttccatcg tcgcccgacc cacaaacaaa cggccgccca
     6001 gccgggcggc cgcctttttg tttgcctgcc ttttctcttt gtgtccaaag ggacctatct
     6061 tgatcgccgc ggccagagga ctggccgaac ggccgaaaaa caattcatcc ccatggccgt
     6121 ggcccaggga tgggccgcat ggccgaaaaa cagccgctcc gcgtctgctt tttcactcct
     6181 tgcagagctc ctcaaacagc gccagcagat aatccagcac ccgcttgaaa gaggccaggt
     6241 ccatcgtctc ctcggtggtg tgcgcgcccg cgcaggtggg cgccaccgtc atgatatcca
     6301 tgtcggggaa tttgcccttg aactcgccgc actcaaggcc gccgtgggtg gcctggatct
     6361 ccatctcgcg gccgaacatc tttttgtaca gcgccatccc gcaggcccgc agcggtgagg
     6421 ccgggtcgta tttccagccg cagaagccgc cggtgcgcac cgcctcaaag cccaacagcc
     6481 cggccagcgt ctcgaactcg ccgcccagct gatcgcccag gctgtcctcg gcggtgcgga
     6541 tgcagtcgca cagatagatc tcgtccggcc tcatagtcag gatgccgata ttgctggagc
     6601 aatgcaccag gccctcgatc tcccggctca taaagcgcat gccgtccggc agcaaaaaga
     6661 gaaaatcgac cagagcgcgg ccgtccccct ccaccagcac ctgggccggc agctccgccg
     6721 cgccgccctc aaaggtgaag cccgggtcgc tgatcttcag ctcctcgcgg atagcggcgg
     6781 ccagcgtttc ggcaatctgc agcgcttcgg ggcccctgcc ggcgggcacg gtgacgaggg
     6841 cggtggcgtc gctgacgatg gcgttcatct tcatgccgcc ctcaaaatgc gccacccgca
     6901 tctccatcgc cgcggccacg gcgtgcagca agcgggccat cagcttgagg ccgttgcccc
     6961 ggccctcgcc gatggtgatg gccgagtggc cgcccagcag gcccgagata tgcagccgca
     7021 ccgcatcgcc gtcggccgcc tcccagcggg gcttcttttt gaaatcatag acgatgccgc
     7081 ccgcgcagga gaccacggtc tgatagtcct cgctggcacc ggagtccatg ttcaccatcc
     7141 ggcgcgcctt gatcagggcc gggtccagcg cttttgcgcc cagcaggccg atctcctcca
     7201 tgctggtgaa gacgaactcc aacggcggac gcacatagtc gtcgcgggcc atcagggcca
     7261 tcatgtgggc gcaggccgtg ccgtcgtccg cccccagcgt ggtgcccacg gcggtcagct
     7321 ggccgtcccg aatctccagc ttcagcgggt ccttttcaaa gtcgtggccg caatcgggca
     7381 gcttgacgca gaccatgtcg gtgtgcccct gcagcatcag cggcggcttg tcctcacagc
     7441 cgggcgaggc gggctttttg accaccacat tgttcgcctc gtccttgtag gcctccagcc
     7501 ccagcccctt ggcgaggtca tagatgtatt gcgccaccgc cgcctcgttg cgcgacccgc
     7561 gcgggatgcg ggacagctcc tcaaaatagc gcagcgcgtc agccggctcc aaccctttgg
     7621 tcacatagtc catttttcac ggcctccctt ttctatcagt ttgcaaaaat tatacccact
     7681 tttggcgcct tgcgcaatgc ccggcaccgc ttttcccctt ttcagcccca aagctgacaa
     7741 aaaccgggcg gcagccccag ccggagcgga gggcttctct ctccggccag ggcgctgccg
     7801 cccggttttt tgtatgagtt ctgaggcccg ccttacagcg cggctttcag ctcctcgacg
     7861 cggtcggtcc gctcccaggt gacgcccagg tcctcgcggc ccatgtgccc gaaagccgcc
     7921 agcgccttgt actgtggccg gcgcaaatcc agcgtgcgga tgatgcccgc gggcgagagg
     7981 tcaaagacct ttttcaccgc ctcggcgatg cggtcgtcct ccaccttgcc ggtgccgaac
     8041 gtgtccacca gcacgctcac cggctgggcc acgccgatgg cgtaggccag ctccagctcg
     8101 catttgctgg caaggccggc cgccacgatg ttctttgcca cccagcgcgc ggcgtaagcg
     8161 ccgctgcggt ccaccttggt ggggtccttg ccggaaaagg ccccgccgcc gtggcggccg
     8221 tagccgccgt aggtgtccac gataatcttg cggccggtca ggcccgagtc gccctgcggg
     8281 ccgcccacca cgaagcggcc ggtggggttg atgagatagc ttgtgccctc atccagcagc
     8341 ccggcgggca caatgggctt taccacctgc tcgatgatct cggcgcgcag ctgctcgatg
     8401 ggtacctccg gcgcgtgctg ggccgacacc acgacggtgt ccacccgcac cggcctgccg
     8461 tcgtcgtact ccacggtgac ctgggtcttg ccgtccggcc gcagatagtc cagcgtgccg
     8521 tcgcggcgca cctcgctcag ccgcttcgcc agcctgtgcg ccagcgagat gggcaggggc
     8581 atcagctccg gcgtctcgtc gcaggcaaac ccgaacatca tcccctggtc gcccgcgccc
     8641 cccacctggt cgttggtgcc catggcgatg tcggggctct gctcgtcgat gttggtgatg
     8701 accgcgcagg tgtcggcgtc aaagccgaac ttggcgcggg tgtagccgat ctcccgcacc
     8761 gtgtcccgca ccagccgcgg gatatccaca tagcaggagg tggtgatctc gcccatgaca
     8821 tggacgaggc cggtgctcac cgccgtctcg caggcgaccc ggccgtccgg gtcctccgcg
     8881 aggatggcgt cgaggatggc gtccgagatc tggtcgcaga tcttgtcggg atgcccctcg
     8941 gtgaccgatt cagaggtaaa aaagcgtttg gacatttcaa tcagacttcc cttctgtttt
     9001 ttgtccaact gaataaccgc ggggcagtct cacttgggac ggtttgaaaa cgggaggagg
     9061 tgggcacacc cgccgcggca tgccgccccc caaaaaagcg ccgttttcag gccgcctcgg
     9121 ggataagaaa aagccccggc aacaaaccgg ggctataaaa agcttatctt ccggtgttac
     9181 ccgcaggatt tggcaccttg cgcagacgcg caggttgtcg ggcttcacag ggcctgttcc
     9241 ctcagccgct cttgataagt gttattcaat tgcggatacc agtttacctc ttttttgtct
     9301 ttctgtcaag gatttctgac aagattccac tttttacccc ggccggtgtc cggagaaaaa
     9361 gggctgcccg gggttcgctt cactcgctct ggacgccgtg ccgctcgcca aagacaaagc
     9421 ggctgatggg ct
//
LOCUS       DBMDYA010000017         9311 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2026_length_9311_cov_1.831053, whole genome shotgun sequence.
ACCESSION   DBMDYA010000017 DBMDYA010000000
VERSION     DBMDYA010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_9311_cov_1.831053"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(110..1579)
                     /locus_tag="ACZPFK_00895"
     CDS             complement(110..1579)
                     /locus_tag="ACZPFK_00895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1404894.1"
                     /translation="MLIQIVENRALAPFLFSLRDIEWFPDLCYSENQCEMARTGGRKG
                     VVMLFTSLEFIGFLAVCVILYYLLPLKFQWYLLLAASLFFYASLGRRRIVFILFSCVT
                     AWAAGLLLGRRHKAEKSAIAELLSPTREEKARTRRRYLLQRRWILAAFLSANLGLWFF
                     FKFYGLAAEKIAVLPHFSLIVPVGISFYTLQITGYLLDVYHKKVEPERSLLKTMLFTT
                     FFPQIIEGPISRFDQLAPQICAPHAFEYDTFVLGLQRILWGYFKKLVIADRFQIAVAT
                     IFNNYTEYYGFEIAIGAILYTIQLYADFSGGIDVALGVGELFGIHLPENFKRPFFSKS
                     VSEFWRRWHITLGTWLRDYIFYPVTLSKPLAKLGRFFSSHKLKWAAKWVPAYLSLLIL
                     WFCNGVWHGDGLQYIAFGLYHGLLIMLGMSFEPLFRRAGGLLHLNYDSMSWRFVRVAR
                     TFLLVCLGELIFRAPGLSAAASMIRNLFSCFNPWVFADV"
     gene            complement(1866..2954)
                     /locus_tag="ACZPFK_00900"
     CDS             complement(1866..2954)
                     /locus_tag="ACZPFK_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404895.1"
                     /translation="MASEHLSIGQNIAALRRQQGVTQEQLASAVGVTAPAVSKWESGQ
                     SCPDIALLAPLARYFGVSVDLLLSYAGPLTPEEALALEERCAAVFERDFAAGLAACRA
                     ALREYPRDGYLAFRLAGLLLGKAVLAQGAEEQQALLDLQRQLLDLALQADDARVRQGA
                     QTLLVSHYMAAGQPEKAEELLSAVPVPDTDPRLMLVSLYQAQGRGEEAEKLAQRLLYD
                     QLCGAMGVLPSLARSALGRGDAAFAACCCDAMDALCRRFKLPANTRYSASLARLTLAL
                     HSKEAEALLAAAQRLVDDQQAILAGGEAPNSPFFDKLEWKIHTFTPKERQAQAVFLQK
                     LLTGEPFAPLKSDPRHAALLQQVEEAMG"
     gene            complement(3704..4591)
                     /locus_tag="ACZPFK_00905"
     CDS             complement(3704..4591)
                     /locus_tag="ACZPFK_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404896.1"
                     /translation="MMEFLNVLGLLLLSAIRTSTPLILAAVGGSVCARSGVMAMGMEG
                     FMLIAAFGAAWGSYAMQNAWLGLLVGIACGVLYSLIFAVLCVTFAMNQVICAIGMNMF
                     AVGFTTSMTQIIWGTRAYSDTVPTLDHIKLPLVGDISVMVLVALASAVLLWAYLFKTP
                     QGLHMRIVGENPIAAKTIGINTKKFKYLGVLICGLLCSLAGSFLSIDHVNRFVMDMTA
                     GRGYIAVSVNILGRFNPLGAIAGGLLFGVADSLQNVVTSSSIPGQLLSMVPYMVTLLV
                     ISFAVRYVSSPAGMGKSED"
     gene            complement(4588..5658)
                     /locus_tag="ACZPFK_00910"
     CDS             complement(4588..5658)
                     /locus_tag="ACZPFK_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404897.1"
                     /translation="MGKFKLDTTKLYHFLRIVAALLLALVFGGAILYVAGFNPLQAYK
                     LIFKGAFGGGKALLTTLIYATPLLFTGLSFIVARHANILNLGIEGQLYAGAMTSALLG
                     AYVTVLPKAVHLPLVLAAAAVAGGLWAALAAFLKVKYGANEVIVTIMMNYVATLFCGY
                     LVAYPFQDEQGLTQTKRIASTAQLGRLVKDHSLSVAILFAIVLIVIINFVFKNSRFGY
                     RVRACGGNRLAAETAGINSRRITIQTMFLSGMISGLCGGLLVTGTYFRFITNFSSGYG
                     FEGVAVATLAAFNPIALILSGILWGGIKSGASAVNRIASVPMDIISLIQALVVILVAA
                     PRLMDKVLAPLKKLLVKGGKTE"
     gene            complement(5645..7174)
                     /locus_tag="ACZPFK_00915"
     CDS             complement(5645..7174)
                     /locus_tag="ACZPFK_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404898.1"
                     /translation="MIEMRGITKRFGALTALDHVDFSLEKGEIHALLGENGAGKTTIM
                     NVLYGMLQPDEGEILVEGRPVRFRDSLDAIQENIGMVSQHFMLVPVLSVTENVVAGLE
                     PRKGLFFDFNRAAEAVEKLARENGFGLDVRVRVSDLSVGECQRVEILKVLYKNAEVLI
                     FDEPTAVLTPQEVDELFVTFRRLREEGKSIVLITHKLGEVMQIADRVTVLRDGRLIGS
                     VNKADTSIRELVKMMVGREVSLGARRRSELGGKVKCSIRNLTYTRGDMPVLSDINIDI
                     REGEIYGIAGIEGNGQTELLEALTGLLKPDSLTLTLDGREITGTAADFLHRGVGHVPE
                     DRLQRGLVSGMSVAENIILGYEGKDAFSKSGLLRRAEIQAASTRLVDDYNIKTPGTGT
                     PVSSLSGGNQQKVIIARVFSEEPDFVVCAQPTRGIDIAASEYIHEVMFDYRDRGKAIL
                     LVSADLDEIKTMSDRIGVLYKGRIVDEDLADNFDDHRLGCRMAGVDYVPESEGDDDGK
                     V"
     gene            complement(7209..8327)
                     /locus_tag="ACZPFK_00920"
     CDS             complement(7209..8327)
                     /locus_tag="ACZPFK_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1404899.1"
                     /translation="MKRFLAVLLSLAMVFSLAACGGTKDLEAGGGSAASGSGAASGGS
                     SAPAPDANAEYKVGVVFGPSGLGDNNFADMIYDGLCRARDELGIYFDYIEPGSDGEII
                     TSLYEYAQDGTYDVVMLATSNAASSLEEVAAEYPDQNFTIIDATVDLPNVRSVAKIGA
                     EQTFMAGVVAALLTQEEGFDGVNKDKKIGVVVGEDFPIPMAMAAGFKAGAYWADPEVE
                     VLVSTVGSWSDPGAAKEMSTAMIKQGVDIIQNDAGGSGTGVFAAAEETGTLAIGVGAN
                     QNSMSDRIIGTSLFILYQYVYNECEALVNGQWTAGVVNPGLADGSLGFVTDGASIEVP
                     QAILDKAEEAKAWVVSGGIELPTKVDGIEEWAAQNGLK"
     gene            complement(8401..>9311)
                     /locus_tag="ACZPFK_00925"
     CDS             complement(8401..>9311)
                     /locus_tag="ACZPFK_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR1404900.1"
                     /translation="TALTDHDCFTDVITESEWEYMRTTCNEFNRDGEFSTLLAYEWTS
                     NEVRYDFGHKNVYYPGGEGEIFRACEKEGLTPDRLFANVKKYGGIAIPHHPAATWEVV
                     SAATDWDFHDPEVQRLAEIFSRHAPFEKKGTTSIYTKNNPRLDGKYVQDALAKGYRLG
                     FTAGSDSHQMEHGTEGGIVAAFVPAQRREDIFAALYNRFVYATTGARILVSLKINGAR
                     MGSEISVCAGGRLRVEIDVLGTRALRSVELVQDNEDVFSPACEGRACRCEQELTLAKG
                     SCFYLRVTQQDEHQAWSSPIWVDVIG"
BASE COUNT         1837 a         2875 c         2920 g         1679 t
ORIGIN      
        1 cttttgttgg caaggtggac ttgcccgaat aaccgcccga cctatccgac acaagagcta
       61 agtgccggat aggaacggca aaatttttta cacttctatt acttctttat cacacatcag
      121 caaacaccca cgggttgaaa caggagaaaa gattgcggat catggaggcg gcggcggaga
      181 gaccgggggc gcggaagatc agctcgccca ggcagaccaa caaaaaggtc ctggccacac
      241 ggacaaatcg ccagctcatg ctgtcatagt tgagatgcag caatccaccc gcccggcgga
      301 aaagcggctc aaagctcatc cccagcatga tcaacaggcc gtgatacagg ccgaaagcaa
      361 tatattgcag gccgtcgccg tgccataccc cgttgcaaaa ccacaagatc aaaagggata
      421 gataagcggg cacccatttt gcggcccatt tcagtttatg agaactgaaa aagcgcccca
      481 attttgccag tggtttgctg agcgtgacgg gataaaagat ataatcgcgc agccaggtgc
      541 ccagtgtgat gtgccaacgt cgccagaact cagaaacgct cttggaaaag aaaggacgtt
      601 tgaagttttc cggcagatgg atgccgaaca actccccaac ccccagggcg acgtctatgc
      661 cgcccgaaaa gtcggcatag agctgaatgg tgtaaagaat tgctccgatc gcaatctcaa
      721 atccgtaata ttctgtataa ttattaaaaa ttgtggccac ggcgatctga aagcggtctg
      781 caatcaccag ctttttgaag tatccccaca aaatgcgctg tagtcccaga acaaaagtgt
      841 catattcaaa ggcgtgtggc gcgcagatct gtggggcgag ctggtcgaaa cggctgatgg
      901 gcccttcgat gatttgagga aaaaacgtgg taaagagcat agttttgaga agactgcgtt
      961 caggctcgac ctttttatgg tagacatcca gcaaatagcc ggtgatctga agcgtataaa
     1021 aagagatccc caccgggacg attagagaaa aatggggcaa gacggcgatc ttttctgcgg
     1081 caagcccgta gaatttaaaa aagaaccaca acccgagatt ggcagataag aaagccgcca
     1141 gaatccagcg gcgctgtagc agataacggc ggcgcgtccg ggctttttcc tctcgtgtcg
     1201 gagaaagaag ctctgcaatc gccgattttt ccgctttgtg tctgcgcccc agcaagaggc
     1261 ccgcagccca ggcggtaacg cagctgaaaa ggataaagac gattcttctg cgccccaggg
     1321 aggcataaaa gaaaaggctt gccgccaaga ggagatacca ttggaatttt aggggcagca
     1381 aataatacaa aataacgcag accgccagaa aaccgataaa ttcgagggat gtgaaaagca
     1441 tgacgacacc tttccgcccg ccggtccggg ccatttcaca ttgattttca ctatagcata
     1501 aatcggggaa ccattcaata tcgcgcagag aaaagagaaa aggcgccagg gctcggtttt
     1561 ctacaatttg gatcaacaaa gagaagagcg cgggatcggc tttgacggcg atctggcgct
     1621 cttctctttt ttataatttg tctgtcttgc gataatacgg cggaatgagc caactgaacg
     1681 gcaacctgcg gcaataggca ataaaagaaa aatgtacatt cctcgcgttg ctctatacgg
     1741 cgcgctttcc cctttcgtgt cgcaccactg gtcaaaagaa agatcaaaat gcagagaagc
     1801 gcaacgggat aatcggtgat ttaaacgatt aaaaagcgtt ggaacagaaa agaatacgag
     1861 cgcgctcagc ccatcgcttc ttccacctgt tgcagcagcg ccgcgtggcg cgggtcgctt
     1921 ttcagcggcg caaagggctc gccggtgagc agcttctgca aaaagaccgc ctgcgcctgc
     1981 cgctcctttg gagtgaaagt gtggatcttc cactccagct tgtcgaagaa agggctgttt
     2041 ggggcttcgc ccccggccag gatcgcctgt tggtcgtcca ccaggcgctg ggcggcggcg
     2101 agcagggcct ccgcttcttt cgagtgcagg gccagcgtca gccgggccag ggaggcgctg
     2161 tagcgggtgt tggccggcag cttaaaacgg cggcagagcg catccatggc gtcgcagcag
     2221 caagcggcga aagcggcgtc tccccggccc agggccgaac gcgcaaggct gggcagtacc
     2281 cccattgccc cgcagagctg gtcatagagc agccgctggg ccagcttctc cgcctcctcc
     2341 ccccggcctt gggcctgata gaggctcacc agcatcaggc gcgggtcggt gtccggcacc
     2401 gggacggcgc tcagcagctc ctccgccttt tccggctggc cggccgccat ataatgagag
     2461 acgagcagcg tctgcgcccc ctggcgcacg cgggcgtcgt cggcctgcag ggccagatcc
     2521 agcagctgcc gctgcaggtc gagcagggcc tgctgttcct ccgcgccctg ggccagcacg
     2581 gccttgccca acagcagccc ggcaaggcgg aacgcaagat agccgtcgcg gggatattcc
     2641 cgcagggcgg cgcggcaggc cgcgagcccg gcggcgaagt cccgctcaaa gactgcggcg
     2701 cagcgctcct ccagcgccag ggcctcctcg ggcgtcaggg ggccggcata agagagcagc
     2761 aggtccaccg agacgccgaa atagcgggcc agcggggcca gcagcgcgat gtccggacag
     2821 ctttggcccg attcccactt gctcaccgcc ggggcggtca cccccacggc ggatgccagc
     2881 tgctcctggg tgaccccctg ctgcctgcgc agcgccgcga tattctgccc gatcgagaga
     2941 tgctctgatg ccatgtgcct gtcctcctgt ggatggatat aaaggcggcc gcctttgtct
     3001 tttattgtac cggggcgggg tgaaaaccgc aatggaggcg cggttaaccc ggcgtcacgt
     3061 tttgttgagc gcgcggcaac cccgatctta tacggcgatc ttgcttttgg gcggcaagtc
     3121 gctttccacc agaacgggac tctccttgct ctcccgcggc agctccgtct cccgcgagaa
     3181 tcaagcttcc cggcagcaac cggctctttg cggcgacttc tctctctgca aagacccctc
     3241 tttccgcaat agctccgcct cccgcggcgg cctgttcttg gcggcaaagc tgtttttcgc
     3301 aagccgccgg tgcgggccgg gaggcgcatg tgcccgtgtg cggacaaggc cgcccttgcg
     3361 gtgaggcaaa ggctgcgggg caggtatctt gcaggggggc gctcgaggcc gtcggggcgc
     3421 ccggcatctc tccggcgccg gggcggcccg acgcctgccg cccatcgtcc ccgcctgatt
     3481 tttcgtataa ggggcagccc ggcgcgacgt cccggacggc gccacagatc tcctttttga
     3541 cgttgccccg gcctgcaggg gacaaaaaag ggcccgcggc caagccgtgt tcggcttggc
     3601 cgcgggcttg aagacgcggt cttgtggtca cggggggaga gaggggctga aaaggcccca
     3661 agggccccgc gcgaggccgc ggcaaaaggg ggcggaatta ggatcagtcc tcgctcttgc
     3721 ccatgccggc gggggagctg acataccgca ctgcaaagga gatcaccagc agcgtcacca
     3781 tatagggcac catggagagc agctgaccag ggatactgga ggaggtgacg acgttctgca
     3841 gcgagtcggc cacgccgaag agcagcccgc ccgcgatggc gcccagcggg ttgaagcggc
     3901 ccaggatgtt caccgacacg gcgatgtagc cgcgcccggc ggtcatatcc atgacaaagc
     3961 ggttgacgtg gtcgatggac agaaagctgc ccgcaaggct gcacagcagg ccgcagatca
     4021 gcacgcccag atacttgaac tttttggtgt tgatgccgat ggtcttggcg gcgatggggt
     4081 tttcgcccac gatgcgcatg tgcaggccct gcggcgtttt gaacagatag gcccagagca
     4141 gcaccgctga ggcgagggcc accagcacca tgaccgagat gtcgcccacc agcggcagtt
     4201 tgatatggtc cagggtgggc acggtgtcgg aataagcgcg ggtgccccag atgatctggg
     4261 tcatcgaggt ggtgaagccc acagcgaaca tgttcatgcc gatggcgcag atgacctggt
     4321 tcatggcgaa agtgacgcag agcaccgcaa agatcagcga gtagagcacg ccgcaggcga
     4381 tgcccaccaa cagccccagc caggcgttct gcatggcata agagccccag gccgcgccaa
     4441 aggcggcgat cagcataaag ccttccatgc ccatggccat cacgccgctg cgcgcgcaga
     4501 ccgacccgcc cacggccgcc aggatcagcg gggtgctggt gcggatcgcg gagagcagca
     4561 gcaggcccag cacgttcaga aattccatca ttccgttttc ccgcctttca caagaagttt
     4621 tttcagcggc gccagcacct tgtccatcag ccggggcgcg gccaccaaaa tgacgaccag
     4681 ggcctggatc agagagatga tgtccatggg cacgctggcg atgcggttga ccgccgacgc
     4741 gccggatttg atgccgcccc agaggatgcc ggagaggatg agggcgatgg ggttgaacgc
     4801 tgccagggtg gccaccgcca cgccctcaaa gccgtagccg gaggaaaagt tggtgatgaa
     4861 gcggaaatag gtgccggtca ccagcagccc gccgcacagg cccgagatca tgcccgacag
     4921 gaacatggtc tggatggtga tgcggcggct gttgatgccc gccgtctcag cggcgagccg
     4981 gttgccgccg caggcgcgca cccggtagcc gaagcggctg tttttaaaga cgaaattgat
     5041 gatcacgatg agcacgatgg cgaacaggat ggccacggac agcgaatggt ccttcaccag
     5101 ccgccccagc tgggcggtgg aggcgatgcg ctttgtctgg gtcaggccct gttcgtcctg
     5161 gaacgggtaa gccaccagat agccgcagaa cagggtggcg acatagttca tcatgatggt
     5221 cacgatgacc tcgttggcgc cgtatttgac cttgagaaag gccgccagcg ccgcccagag
     5281 cccgcccgcc acggcggcgg ccgccagcac caggggcagg tggacggcct tgggcagcac
     5341 cgtgacgtag gcgcccagca gagccgaggt catcgccccg gcgtagagct ggccctcgat
     5401 gcccagattg aggatgttgg cgtgccgggc cacgataaag gacagcccgg taaagagcag
     5461 cggggtggcg tagatgaggg tggtgagcag cgccttgccg cccccgaacg cgcccttgaa
     5521 gatcagcttg taggcctgca gcgggttgaa cccggccaca tagaggatgg cgccgccaaa
     5581 gaccagcgcc agcagcagag ccgccacgat cctcaaaaag tggtacagct ttgtggtgtc
     5641 gagtttaaac tttcccatcg tcatccccct cgctctccgg gacatagtcc acgcccgcca
     5701 tgcggcagcc cagccggtgg tcgtcgaaat tgtccgccag gtcctcgtcc acgatgcggc
     5761 ccttatacag cacgccgatg cggtcgctca tcgtcttgat ctcgtccagg tcggccgaga
     5821 ccagcaggat ggccttgccc cggtcccggt agtcgaacat cacctcgtgg atatactccg
     5881 acgccgcgat gtcgatgccg cgggtgggct gggcgcagac cacgaaatcc ggctcctccg
     5941 agaagacccg ggcgatgatc accttttgct ggttgccgcc cgacagggag gacaccggcg
     6001 tgcccgtgcc cggcgtcttg atgttgtagt cgtccaccag cctggtgctg gccgcctgga
     6061 tttcggcgcg ccgcagcagg ccggatttgg aaaaggcgtc cttgccctca tagcccagga
     6121 tgatgttttc ggccaccgac atgccggaca ccaggccgcg ctgcagccgg tcctcgggca
     6181 catggcccac gccgcggtgc agaaaatccg ccgcagtgcc cgtgatctcc cggccgtcca
     6241 gcgtcaaggt gagggagtcc ggcttcagca ggccggtgag agcctccagc agctcggtct
     6301 ggccgttgcc ctcgatgccg gcgataccgt agatctcgcc ctcccggatg tcgatgttga
     6361 tgtcggagag caccggcata tcgccgcggg tgtaggtcaa attgcggatg ctgcacttga
     6421 ccttgccgcc gagctccgag cggcgcctgg cccccaggct cacctcgcgg cccaccatca
     6481 tcttgaccag ctcgcggata gaggtgtccg ccttgttcac gctgccgatg aggcggccgt
     6541 cccgcagcac ggtgacccgg tcggcgatct gcatgacctc gcccagcttg tgggtgatga
     6601 gcacgatgct cttgccctcc tcccgcaggc ggcggaaggt gacgaacagc tcgtccacct
     6661 cctggggggt gagcacggcg gtgggctcgt cgaaaatcag cacctcggcg tttttgtaca
     6721 gcactttcag gatctcgacc cgctggcact cgcccaccga caggtcgctg acccgcaccc
     6781 gcacatccag gccaaagccg ttttcccgcg ccagcttttc cacggcctcg gcggcacggt
     6841 taaagtcgaa gaacagcccc ttgcgcggct ccagccccgc gacgacgttt tcggtgaccg
     6901 agagcaccgg caccagcatg aagtgctggc tcaccatgcc gatgttttcc tggatggcgt
     6961 ccagtgaatc ccgaaaacgc acaggcctgc cctccaccag aatttcgccc tcgtcgggct
     7021 gcagcatgcc gtacagcacg ttcatgatcg tcgttttgcc ggcgccgttc tcgccgagaa
     7081 gggcgtggat ttctcccttt tccagggaga aatccacatg gtccagtgcg gtcagggcgc
     7141 caaacctctt ggtgatgccg cgcatttcaa tcatactcac tgtaatgccc tccacacgtc
     7201 aacacggttt atttgaggcc gttctgggcg gcccactcct cgatgccgtc caccttggtg
     7261 ggcagctcga tgccgccgga gacgacccac gctttcgctt cctccgcctt gtccaggatc
     7321 gcctgcggca cttcgatgct ggcgccgtcg gtcacaaagc ccagcgagcc gtcggccagg
     7381 ccagggttca cgacgccggc ggtccactgt ccgttgacca gcgcctcgca ctcgttgtaa
     7441 acatactggt agaggatgaa cagcgaggtg ccgatgatcc ggtcgctcat cgagttctgg
     7501 tttgcgccca cgccgatggc aagcgtgccg gtctcctcgg ccgccgcaaa gacgccggtg
     7561 ccggagccgc ccgcgtcgtt ctggatgatg tcgacgccct gcttgatcat ggcggtagac
     7621 atctccttgg cggcgccggg atcgctccag gagcccacgg tggagaccag cacctcgacc
     7681 tcagggtccg cccagtaggc gcccgccttg aagcccgcgg ccatcgccat ggggatgggg
     7741 aaatcctcgc ccaccacgac gccgatcttt ttgtctttgt tgacgccgtc aaagccctct
     7801 tcctgggtga gcagggcggc caccacaccg gccataaagg tctgctcggc gccgatcttg
     7861 gccaccgagc gcacattggg caggtccacc gtggcgtcga tgatggtgaa gttctggtca
     7921 gggtattcgg cggcgacctc ctccagcgag gaggcggcgt tggaggtggc cagcatgacc
     7981 acgtcgtagg tgccgtcctg cgcgtactcg tacagggagg tgatgatctc gccgtcggag
     8041 ccgggctcga tgtagtcgaa atagatgccc agctcgtcgc gggcgcggca gaggccgtcg
     8101 tagatcatgt ccgcaaagtt gttgtcgccc aggcccgacg ggccgaagac cacgcccacc
     8161 ttgtactcgg cgttcgcgtc gggcgccggg gcggaggagc cgccggaggc cgcacccgag
     8221 cccgaggccg cgctgccgcc gcccgcctcc aagtccttgg tgccgccgca ggcggccagg
     8281 gagaacacca tggccagtga caagagaacc gctaaaaacc gtttcatttt cttccctcca
     8341 tctttcctta tcctgtggtg aatatttttg agtgcgcacc gcgcgcaggg gatacttggt
     8401 tcagccgatc acgtccaccc agatggggct ggaccaggcc tggtgctcgt cctgctgggt
     8461 gacgcgcagg tagaagcagc tgccctttgc cagcgtcagc tcctgctcgc agcggcaggc
     8521 gcggccctcg caggcggggg agaacacgtc ctcgttgtcc tgcaccagct ccacgctgcg
     8581 cagcgcgcgg gtgcccagca cgtcgatctc caccctgagc cgcccaccgg cacagacgct
     8641 gatctcgctg cccatgcggg cgccgttgat tttgagcgag accagaatgc gcgcgccggt
     8701 ggtggcgtag acaaagcggt tgtacagggc ggcaaagatg tcctcgcggc gctgggcggg
     8761 cacgaatgcg gccacaatgc cgccctcggt gccgtgctcc atctggtgcg agtcgctgcc
     8821 ggcggtgaag cccaggcggt accctttcgc cagcgcgtcc tgcacatact tgccgtccag
     8881 ccgcgggttg tttttggtgt agatcgaggt ggtgcccttt ttctcaaaag gggcgtggcg
     8941 ggagaagatc tcggccaagc gctgcacctc cgggtcgtga aagtcccagt cggtggccgc
     9001 gctcaccacc tcccaggtgg cggcggggtg atgggggatg gcgatgccgc cgtatttttt
     9061 gacgttggcg aagaggcggt ccggcgtcag gccctctttt tcacaggcgc ggaagatctc
     9121 cccctcgccg cccgggtagt agacgttctt gtggccaaag tcgtaccgca cctcgttgga
     9181 ggtccactcg taggccagca gggtggagaa ctcgccgtcc cggttgaact cgttgcaggt
     9241 ggtgcgcatg tactcccact ccgactcggt gatgacgtcg gtgaagcagt cgtggtcggt
     9301 cagggcggtg a
//
LOCUS       DBMDYA010000018         9172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2057_length_9172_cov_1.511630, whole genome shotgun sequence.
ACCESSION   DBMDYA010000018 DBMDYA010000000
VERSION     DBMDYA010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_9172_cov_1.511630"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="ACZPFK_00930"
     CDS             <1..1569
                     /locus_tag="ACZPFK_00930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1404901.1"
                     /translation="LSATPGALADPLPEALRSRYRLPELAAALRGIHFPSGRAEADGA
                     RRRFAFEELFCLELGMKLLRSRNRRASGAPMARCDPEAFYASLPFAPTGAQRRAVEEL
                     CRDFGGEAPMNRLLQGDVGSGKTLVAAAGMAMAAANGYQSVLMAPTEILAVQHAATLE
                     RMLRPLGLRVALLTGSVKGKARKALLAAVAAGEAEVVVGTHAVLGKDVRFARLGFAVV
                     DEQHRFGVRQRGLLAEKAEDPHLLVMSATPIPRTLSLLLFGELDVSILDELPPGRTPV
                     RTLAVGTALRARMYGFLEKQIAAGRQVFIVCPLIEQGDDASPAAAEMQAVTAYREEVA
                     ARLLPGRRIGLLHGRLKAAEKAEVMARFAAGELDVLCSTTVVEVGVDVPNASVMVIEN
                     AERYGLSALHQLRGRVGRGAAESFCILVSDHDGPQVKERLSFLCHTSDGFAVAQYDLE
                     HRGPGDFFGRRQHGLPTLRVADAASDARMIETAAAEAEALLAEDPALDAPAHAALRRE
                     VDGLFSEAGGTAVN"
     gene            1707..2429
                     /locus_tag="ACZPFK_00935"
     CDS             1707..2429
                     /locus_tag="ACZPFK_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR1404902.1"
                     /translation="MFLGTIVNAAAVVAGGLLGLLFKKGMKPSLEESIHKALGVAVLV
                     LGLNGIISSMFTVSAEGKPSSSGELLLIVSLVLGMLAGELLRIDERLNSFGRRIEQKV
                     GTAGFAASFVNGTLIFCVGAMTIVGALNDGLYHDPSILYVKSMLDAIGAVVFGATLGV
                     GVCFCAIPILLYEGGLTLLAGLLAPYLQGALLDQICMVGYAMVACIGINFISKEAKIK
                     TANLLPALLVPVAWSFIQGLFA"
     gene            3327..4550
                     /locus_tag="ACZPFK_00940"
     CDS             3327..4550
                     /locus_tag="ACZPFK_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1404903.1"
                     /translation="MKKFVCTALAVCMALSLVACGGSGSSSSAAAGSSAAGGSSAAAS
                     TGEGGSIKVGLLANTSDANANYGNAVKNGAMLYIDEVNAAGGVNGKQIEVVAYDDKGE
                     ATEAVTAFNRLLDDGVTAVIGSVLTGTTLAVADESFAVGMPQITASATAPGVTLLDPE
                     DESAGIRDNVFRACFIDPFQGEKMAEYAAEKMGAKTAAVIFETGNDYSEGLKNAFVEK
                     CAELGVTVTNEEAYSTGDVDFKAQMTNIAKSAPDVVFSPNYYEDDGKIVTQARQLGYE
                     GTFLGGDGWSGVGPYASAEDLEGTVYCSAYAGDEKFESAYEAAYGEKVPNMFAPLGYD
                     AAKLMVAALGEAEDQGLEAGSDEYKAAVIAALKATDGVEGVTGSYRFDEFNNPIKSAA
                     MMQLQSGKEVFTEMF"
     gene            4737..5615
                     /locus_tag="ACZPFK_00945"
     CDS             4737..5615
                     /locus_tag="ACZPFK_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1404904.1"
                     /translation="MSTLFSQAVNGLQSGSIYALVALGYSMVYGIIMLLNFAHGDIIM
                     VGAYVVWMATANFGLHPLVSILLAVAVSTLLGVVIEKVAYAPLRSSPRLSLLITAIGI
                     SFLLENGFQLIFGAGAKVVPEFLSGPSLKLGSATVSFSAMVTLVVTVAIMIGLTLLVQ
                     KTRLGKAMRAVSEDMGAAQLMGINLNTTISFTFALGSALAAVASVLYLSSYAQASPTM
                     GSMLGLKAFVAAVLGGIGSLPGAMLGGFVIGLAEALVSAVGLSVWKDGVVFAILIVVL
                     LVRPSGLLGKPMKEKV"
     gene            5629..6678
                     /locus_tag="ACZPFK_00950"
     CDS             5629..6678
                     /locus_tag="ACZPFK_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1404905.1"
                     /translation="MQTKLNHKTIPMPARYLINAALIALFLFGGNAFVGSGAVSGYFI
                     KVLMLLGINVILAVSLNVATGYLGQLPLGHAGFMAVGAYTGGIFLKAVEADKAGGVQY
                     ALYVGAALLLSGVVAGVFGILIGIPALRLRGDYLAIITLGFGEIIRVVLTNIDEVLGF
                     KFTYGASGLKRIPKITSFFNVALMVVLTCLVIHMVMKSRHGRAILSIRENEIAAESCG
                     IRTTYYKVMAFTLSAFFAGVAGCLYAGYLGSLQPTTFGFMKSIEILVMVVLGGMGSML
                     GSVLSATVLTALPEALRAFSDYRMVVYSLVLVLTMIFKPTGLLGQYDFSLSRILEKLM
                     NPKAKRQKKEGGADE"
     gene            6671..7519
                     /locus_tag="ACZPFK_00955"
     CDS             6671..7519
                     /locus_tag="ACZPFK_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404906.1"
                     /translation="MSKEKTKLVEVPGGIIPERDAGAPPVLEVRHLGIDFGGLTAVDD
                     FNFIIGRTEIGGLIGPNGAGKTTIFNLLTNVYQPTRGHILVNGVDTRGKKTYEVNKMG
                     IARTFQNIRLFKALSVLDNVLIGMHNALGYRLGASVLRLPSFWGQERTARAKALELLS
                     LFGMQELADVQAGNLPYGAQRRLEIVRALASGPKVLLLDEPAAGMNPSETAELMENIR
                     RIRDTFQIAIILIEHDMNLVMGICEGIAVLNYGQIIAKGTPEEIRTNPVVIEAYLGKK
                     GGAAGC"
     gene            7513..8214
                     /locus_tag="ACZPFK_00960"
     CDS             7513..8214
                     /locus_tag="ACZPFK_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1404907.1"
                     /translation="MLKVENINVYYGNIHAVKDISFEVEQGEIVTLIGANGAGKSTIL
                     NTISGLLRSKTGSVTFEGKPIGSLPPHKVLALGLAQVPEGRRVFLKMTVEENLEMGGY
                     TRPAGELAASVEDVYRRFPRLEERRRQVAGTLSGGEQQMLAMGRALMAKPALMMLDEP
                     SMGLAPLLVEQIFDIIKELHQAGTTILLVEQNAQMALSVADRAYVLETGRIVMQGDAR
                     ALLEDDQVRRAYLGE"
     gene            8427..8927
                     /locus_tag="ACZPFK_00965"
     CDS             8427..8927
                     /locus_tag="ACZPFK_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1404908.1"
                     /translation="MDVSDFEEIYRLYFQDVYKFLFALCRDRALAEELTQDTFFKAMK
                     SCGSFRGDCKITTWLCQIAKNTLFLHQKQRRRESGPEPEESAPDPGEPVELLFDQRDQ
                     ALKIHRVLHWLPEPYKEVFSLRILGELSFRDIGALFCKTEGWARVTFHRAKLKLTTRL
                     KEKNDG"
     gene            8920..>9172
                     /locus_tag="ACZPFK_00970"
     CDS             8920..>9172
                     /locus_tag="ACZPFK_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024881.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR1404909.1"
                     /translation="MDEILCDVIQDLLPLYCDGVCSEESKKLVEKHLRNCETCTDLLQ
                     RMKTECQAVPAGGNEHEAAPLKKMARTWRRSLTRSFLKGV"
BASE COUNT         1555 a         2973 c         3045 g         1599 t
ORIGIN      
        1 ctgagcgcca cgccgggggc gctggcggac cctctgcccg aggcgctgcg cagccgctac
       61 cgtctgccgg agctggcggc ggcgctgcgg ggcatccact tcccctccgg ccgcgccgag
      121 gcggatgggg cgcggcggcg gtttgccttt gaggagctgt tctgcctgga gctgggcatg
      181 aagctgctgc gcagccgcaa ccggcgcgcc agcggcgcgc cgatggcccg ctgcgacccg
      241 gaagcgtttt acgcctcgct gccctttgcc cccaccggcg cgcagcggcg ggcggtggaa
      301 gagctctgcc gcgactttgg cggcgaggcc cccatgaacc gcctcttgca gggcgacgtg
      361 ggcagcggca aaacgctggt ggccgccgcg ggcatggcga tggcggcggc gaacggctat
      421 cagagcgtgc tgatggcccc caccgagatc ctggccgtcc agcacgccgc caccctggag
      481 cggatgctgc gccccctggg gcttagggtg gcgctgctca ccggctcggt gaagggcaag
      541 gcgcgcaagg cgctgctggc cgccgttgcc gccggcgagg cggaggtggt ggtgggcacc
      601 cacgcggtgc tgggcaagga cgtgcgcttt gcccgcctgg gcttcgcggt ggtggatgag
      661 cagcaccgct tcggggtgcg ccagcgcggg ctgctggcgg aaaaggccga ggacccgcac
      721 ctgctggtca tgagcgccac ccccatcccc cgcaccctga gcctgctgct gttcggcgag
      781 ctggacgtct ccatcctgga cgagctgccc ccgggccgca cgccggtgcg caccctcgcc
      841 gtgggcaccg cgctgcgggc gcggatgtat ggctttttgg aaaagcagat cgccgcgggc
      901 cgccaggtct ttatcgtctg ccccctcatc gagcaggggg acgacgccag ccccgccgcc
      961 gccgagatgc aggcggtgac cgcttaccgg gaggaggtgg ccgcgcggct gctgcccggg
     1021 cggcgcatcg gtctgctgca cgggcgtctg aaagcggccg aaaaggccga ggtcatggcg
     1081 cggtttgcgg ccggggagct ggacgtgctc tgctccacca cggtggtgga ggtgggggtg
     1141 gatgtgccca acgccagcgt catggtgatt gagaacgccg agcgctacgg cctgtcggcc
     1201 ctgcatcagc tgcgcggccg ggtgggccgc ggcgcggccg agagcttctg catcctggtc
     1261 agcgaccacg acggcccgca ggtgaaagag cggctgtcct ttctctgcca cacctcggac
     1321 ggcttcgccg tggcccaata cgacctggag caccggggcc ccggcgactt tttcggccgc
     1381 cggcagcacg gcctgcccac cctgcgggtg gcggacgcgg cctccgacgc gcggatgatc
     1441 gagacggcgg ccgcggaggc ggaggccctg ctggccgaag acccggccct ggacgccccg
     1501 gcccacgccg ccctgcgccg tgaggtggac ggcctgttca gcgaggccgg gggcacggcg
     1561 gtcaactgaa cggccgcgcg gcgggccgcc gggcggggct cacccggccc taccgccccc
     1621 tctccgcgcg ccccgccggg cgcggcgggg cgccgttttt ccgccgcccc gcaaaaaaag
     1681 tttgcctgca aaaaaggagc gatatcatgt ttctgggtac cattgttaac gccgccgccg
     1741 tggtggcggg cggcctgttg ggtcttctct tcaaaaaagg gatgaagccc tcgctggagg
     1801 aatccatcca caaggcgctg ggggtggcgg tgctggtgct gggcctcaac ggcatcatct
     1861 cctccatgtt caccgtctcg gcggagggca agccctcctc ctccggcgag ctgctgctca
     1921 tcgtcagcct ggtgctgggc atgctggcgg gcgagctgct gcgcatcgac gagcggctga
     1981 acagcttcgg ccggcgcatc gagcaaaagg tgggcaccgc gggcttcgcc gcctcctttg
     2041 tcaacggcac cctgatcttc tgcgtggggg ccatgaccat tgtgggcgcg ctcaacgacg
     2101 gcctctacca cgaccccagc atcctctatg tcaagagcat gctggacgcc atcggcgcgg
     2161 tggtctttgg ggccacactg ggggtggggg tctgcttttg cgccatcccc atcctgctct
     2221 atgagggcgg gctcaccctg ctggcgggcc tgctggcgcc ctatctgcag ggcgcattgc
     2281 tggaccagat ctgcatggtg ggctacgcga tggtggcctg catcggcatc aacttcatca
     2341 gcaaggaggc caagatcaag accgccaacc tgctgccggc cctgttggtg ccggtggcgt
     2401 ggagctttat ccagggactt tttgcctgaa ggagcctgcc cggccagagc ccgggcacac
     2461 cgggccaaag ggacaaaaac aaaaacgcgg cgggtcctcg gggagagggc ccgccgcgtt
     2521 ttgccgtccc gctcgcgcgg aatgcagagg gcgctttgcg cctcatgcag ggcgcgcggc
     2581 gcgcgggcac gccccctcgg caggaatgag ccccataggc tacggacgcg gcccgggggg
     2641 cacagacgcg ggggcacacg gcgcgcggat gcgaccctgt gcatccagat cggggaccca
     2701 ggacgcataa gagcgggtcg gcgctgcgcc ggggatggga ggggaagcgg cggcgccccc
     2761 gcagccgggg cagtggcgcg ccgtgtcttt tcaaaccgcg cggcctgcgg cagagcctcc
     2821 gccccggcgg agctttcaaa aatggccccg cggattttgc ccgtccctct gcgccgcgct
     2881 tccctctccg attttcaagc tgcgcgttct gcggccctct gcccagggcc gccgccccgg
     2941 cgaacctttt caaaaggggc cccacgggtt ttgcccgtct ctctgcgccg tgcctgcctc
     3001 cctccggcag gcggtttccg cttcctgctt cacctcgccc gcccctgtgg gggccgcctt
     3061 tcacctttgt cccgcgggcg gttttctctg atttgttaca aaagtgtgtc aaaatgaggt
     3121 gcatttttta aaaaattgct ttcgttttga tcaaatgcgc caaagccgcg ccgctgtttt
     3181 cagcaaaatg ttgtaaaacc catgcattgc gcctccctgt gacctgagct ataatgggca
     3241 acaatcactt ccgcagcggg ccgccgcaaa cagaggagaa gagaagggcg gccaggcgga
     3301 ggaaaagaaa acaggaggta agaggtatga aaaagtttgt ctgcactgca ctggcggtct
     3361 gcatggccct ttcgctggtc gcctgcggcg ggtccggctc cagcagctcg gccgcggccg
     3421 gcagcagcgc cgcgggcggt tcgtccgcgg ccgcatccac gggggaaggc ggttccatta
     3481 aagtcggtct gctggccaac acctccgacg cgaacgccaa ctacggcaac gccgttaaaa
     3541 acggcgccat gctctatatc gatgaggtca acgccgccgg cggcgtgaac ggcaagcaga
     3601 tcgaggtcgt cgcctacgac gataagggcg aggccaccga ggcggtcacc gctttcaacc
     3661 gcctgctgga cgacggcgtc accgccgtga tcggctcggt gctgaccggc accaccctgg
     3721 cggtcgccga cgagagcttc gccgtgggca tgccgcagat caccgcctcc gccaccgcgc
     3781 ccggcgtcac cctgctggac ccggaggatg agagcgccgg catccgggac aacgtcttcc
     3841 gcgcctgctt catcgacccg ttccagggcg agaagatggc ggaatacgcc gccgaaaaga
     3901 tgggcgccaa aaccgccgcc gtcatctttg agaccggcaa cgactactcg gagggcctga
     3961 aaaacgcctt tgtggaaaaa tgcgccgagc tcggcgtcac cgtcaccaac gaggaggcct
     4021 actccaccgg cgacgtggat ttcaaggccc agatgaccaa catcgccaag tccgcccccg
     4081 acgtggtctt ctccccgaac tactatgagg acgacggcaa gatcgtcacc caggcccgcc
     4141 agctcgggta tgagggcact ttcctgggcg gcgacggctg gagcggcgtc ggcccctacg
     4201 cctccgccga ggatctggag ggcacggtct actgctccgc ctacgcgggc gacgaaaagt
     4261 tcgagtcggc ctatgaggcc gcctacggcg aaaaggtccc caacatgttc gcgcccctgg
     4321 gctacgacgc ggccaagctg atggtcgccg cgctgggcga ggcggaggac cagggcctgg
     4381 aggccggcag cgacgagtat aaggccgcgg tcatcgccgc gctgaaagcc accgacggcg
     4441 tcgagggcgt caccggcagc tacaggttcg acgagttcaa caaccccatc aagtccgccg
     4501 ccatgatgca gctgcagagc ggcaaagagg tcttcaccga gatgttctaa aacaggcctc
     4561 atcagcggaa tcacgacctt gcaagaacta tgcggggagg gcaaccctct ctcaccgcat
     4621 agtttcttgt tatctcgggc ggggccatcc ggcgtccgcc cgcacggctc caacttgatc
     4681 caatcagcgc cgccagggtg tgcggcgcag aaagagaggc gcagaacatg gcgttcctgt
     4741 ccacattgtt cagccaggcc gtcaacggcc tgcagtccgg ctcgatctac gcgctggtcg
     4801 cactggggta cagcatggtt tacggcatca tcatgctgct caactttgca cacggcgata
     4861 tcattatggt aggcgcttac gtcgtatgga tggcgaccgc caacttcggc ctccatcccc
     4921 tcgtctccat cctgctggcg gtggccgtca gcacgctgct gggcgtggtg atcgaaaagg
     4981 tggcctatgc cccgctgcgc tcctcgccgc ggctctcgct gctgatcacc gccatcggca
     5041 tctcgttttt gctggaaaac ggctttcagc tgatcttcgg cgcgggggcc aaggtggtgc
     5101 ccgagttctt gtccggcccc tctctcaagc tggggagcgc cacggtcagc ttttccgcca
     5161 tggtcacgct ggtggtgacg gtggccatca tgatcggcct gaccctgctg gtgcagaaga
     5221 cccgcctggg caaggcgatg cgggcggtga gcgaggatat gggcgccgcc cagctgatgg
     5281 gcatcaacct gaacaccacc atctctttca cctttgcgct gggctcggcg ctggcggcgg
     5341 tggcctcggt gctctacctg tcctcctacg cccaggcctc gcccaccatg ggcagcatgc
     5401 tggggctgaa agcctttgtg gcggcggtgc tgggcggcat cggcagcctg ccgggcgcga
     5461 tgctgggcgg ttttgtcatc ggcctggcgg aggcgctggt ctcggccgtg ggcctctcgg
     5521 tgtggaagga cggcgtcgtc ttcgccatcc tgatcgtggt attgctggtg cggcccagcg
     5581 gcctgctggg caagcccatg aaagaaaaag tataaggggg cgcgcggaat gcaaacaaaa
     5641 ctgaaccata agaccatccc catgcccgcc cgctacctga tcaacgccgc cctcatcgcc
     5701 ctgttcctgt tcggcggcaa cgcttttgtg ggcagcggcg cggtgtccgg ctactttatc
     5761 aaggtgctga tgctcctcgg catcaacgtc atcctggccg tgtctttgaa cgtcgccacc
     5821 ggctacctgg ggcagctgcc gctggggcac gccggcttta tggccgtggg cgcctacacc
     5881 gggggcatct ttctcaaggc ggtggaggcc gacaaggccg gcggcgtcca gtacgccctt
     5941 tatgtggggg cggcgctgct gctctcgggc gtcgtggcgg gcgtcttcgg catcctcatc
     6001 ggcatcccgg ccctgcggct gcggggcgac tatctcgcta ttatcacgct gggctttggc
     6061 gagatcatcc gggtggtgct gaccaacatc gacgaggtgc tgggcttcaa gttcacctac
     6121 ggcgcctccg gcctcaagcg catccctaag atcaccagct ttttcaacgt ggcgctgatg
     6181 gtggtgctca cctgcctggt catccacatg gtcatgaaat cccgccacgg ccgggcgatc
     6241 ctctcgatcc gcgaaaacga gatcgccgcc gagagctgcg gcatccgcac cacctattac
     6301 aaggtgatgg ctttcacgct gtcggccttt ttcgccgggg tggccggttg tctctacgcg
     6361 ggctatctgg gcagcctgca gcccaccact ttcggcttta tgaaatccat cgagatcctg
     6421 gtcatggtgg tgctgggcgg catgggctcc atgctgggct cggtcctctc ggccaccgtg
     6481 ctcacggccc tgcccgaggc gctgcgcgcc ttttccgact accgcatggt ggtctactcg
     6541 ctggtgctgg tgctgaccat gatcttcaag cccaccggcc tgctggggca gtatgacttc
     6601 tcgctgtccc gcatcctgga aaagctgatg aaccccaagg ccaaacggca gaaaaaggag
     6661 ggcggcgcgg atgagtaagg aaaagacaaa gctcgtcgag gtgccgggcg gcatcatccc
     6721 cgagcgggac gcgggcgcgc cgccggtgct ggaggtgcgg cacctgggca ttgatttcgg
     6781 cggcctgacc gcggtggacg acttcaactt catcatcggc cgcaccgaga tcggcggcct
     6841 gatcggcccc aacggcgcgg gcaagaccac catcttcaac ctgctgacca acgtctacca
     6901 gcccacccgc ggccacatcc tcgtcaacgg ggtggacacc cggggcaaaa agacctatga
     6961 ggtcaataag atgggcatcg cccgcacctt tcaaaacatc cggctgttca aggccctgtc
     7021 ggtgctggac aacgtgctga tcggcatgca caacgccctg ggctaccgcc tgggcgcgtc
     7081 ggtgctgcgg ctgccctcgt tctgggggca ggagcgcacc gcccgcgcca aggcgctgga
     7141 gctgctgtcc ctcttcggca tgcaggagct ggccgacgtg caggcgggca atctgcccta
     7201 cggcgcccag cggcggctgg agatcgtgcg ggcgctggcc tccgggccca aggtgctgct
     7261 gctggacgaa cccgcggccg gcatgaaccc ctccgagacc gccgagctga tggagaacat
     7321 ccgcaggatc cgggacacct ttcagatcgc gatcatcctc atcgagcacg acatgaacct
     7381 ggtcatgggc atctgcgagg gcatcgcggt gctgaattac ggccagatca tcgccaaggg
     7441 cacaccggag gagatccgca ccaatccggt ggtcatcgag gcctatctgg gcaagaaagg
     7501 gggcgcggcc ggatgctgaa agtggagaac atcaacgtgt actacggcaa tatccacgcc
     7561 gtgaaagaca tcagcttcga ggtggagcag ggcgagatcg tcaccctgat cggcgccaac
     7621 ggcgcgggca agagcaccat cctgaacacc atctccggcc tgctgcgctc caagaccggg
     7681 tcggtcacct ttgagggcaa gcccatcggc agcctgcccc cgcacaaggt tctggccctg
     7741 gggctggccc aggtgccgga gggccgccgt gtctttttga agatgacggt ggaggagaac
     7801 ctggagatgg gcggctacac ccgcccggcc ggtgaactcg ccgcctcggt ggaggacgtc
     7861 taccgccgct ttccccggct ggaagagcgc cgccggcagg tggcgggcac cctgtccggc
     7921 ggcgagcagc agatgctggc catgggccgg gcgctgatgg caaagcccgc cctgatgatg
     7981 ctggacgaac ccagcatggg gctggcgccg ctgctggtgg agcagatctt cgacatcatc
     8041 aaggagctgc accaggcggg caccaccatc ctgctggtgg agcagaacgc ccagatggcg
     8101 ctgtcggtgg ccgaccgggc ctacgtgctg gagaccggcc gcatcgtcat gcagggcgat
     8161 gcgcgcgcgc tgctggagga cgaccaggtg cgccgggcct atctgggtga atgagcggcc
     8221 ccaaaaccga aaaggacctt ttgagatgac gcctgcgggc gtcatttttt tgtttctttt
     8281 gccgccgctc cccctgcttc acgcggcccc tccccgagtc tgtggccgca tcgccgggct
     8341 tttttgcaaa ttgaggacaa aaagcgtaag aagcctgtaa caaaagcctc cctccctgct
     8401 cttactgaca gaaagggggt gaaagggtgg atgtttccga cttcgaggag atttaccggc
     8461 tgtacttcca ggacgtctac aaatttctat tcgctctctg ccgggatcgg gcgctcgcgg
     8521 aagagctgac gcaggacact tttttcaaag cgatgaagag ctgcggcagc ttccggggtg
     8581 actgcaaaat cacgacctgg ctgtgtcaga tcgccaaaaa cacgctgttt ctgcatcaaa
     8641 agcagcgcag gcgcgaatcg ggcccggaac cggaggagag cgcacccgac cccggggagc
     8701 cggtggagct tctcttcgat cagcgggacc aggccctgaa gatccaccgc gtcctgcact
     8761 ggctgccgga gccttataaa gaggtgttct ctctgcgcat tctgggggaa ctgtctttcc
     8821 gggacattgg ggcgctgttc tgcaagaccg agggctgggc aagagtgact tttcacaggg
     8881 ccaagctgaa actgacgact cgactaaagg agaaaaacga tggatgaaat tttgtgcgat
     8941 gtgatccagg acctgctgcc gctttattgc gacggtgtct gctccgagga gagcaaaaag
     9001 ctcgtggaaa agcatcttcg caactgcgag acctgcaccg acctgctgca acggatgaaa
     9061 acggagtgtc aagccgtccc tgccgggggg aatgaacacg aagccgcgcc gctgaaaaaa
     9121 atggcccgca catggcgccg ttctctcaca cgctcttttt tgaaaggggt tt
//
LOCUS       DBMDYA010000019         9131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2069_length_9131_cov_1.388483, whole genome shotgun sequence.
ACCESSION   DBMDYA010000019 DBMDYA010000000
VERSION     DBMDYA010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_9131_cov_1.388483"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="ACZPFK_00975"
     CDS             <1..452
                     /locus_tag="ACZPFK_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR1404910.1"
                     /translation="RVVDGVVESAEVVKVEDVVPAQDTVIMRYGAGAPVSQLEGPAGV
                     TVENGVPSSYRQVLTGRATAYSASRGRGSSGLGLYEGTVAVDPNVIPYGSLLYITSTD
                     GRFVYGYAVATDTGSAMVEGHALVDLFYDTFDEAYLSAVQSVNVYIL"
     gene            complement(949..2532)
                     /locus_tag="ACZPFK_00980"
     CDS             complement(949..2532)
                     /locus_tag="ACZPFK_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1404911.1"
                     /translation="MTKRLAALALAGALCLCLLTGCKKDAVNFIYYTSDAIATLDPQL
                     ASTPAELTAVKNMFAGLYRLDGEGRAVPDRARGVEISADGLVYTFTLDPEDVFTDGGE
                     ITVPVTAKDYVFALRRLLDPATGAPSARSFLSLRGAAEVLAGAARPESLGVRAVSDTV
                     LEIALAAPDDGLPAKLATAGAMPCNEEFFDSTAGAYGLSVKAILGNGAFQPTGWSQAN
                     GLTLRRLRGDEGDLVNRIRLVPDDGGSTAAARLADGLQDGALAAADQETQLAGYPSQT
                     FETTVWTLVFNCADEALSNLSIRQALASVAQLSGEDLAGLGHLRAADGLVPGSARLTG
                     GGGYRDAVGGALPSLAEAQSYSLYRLGLSELGVERLSGLQVLIPETEPWGELYPIINQ
                     RWQRSLSAFFSVQALPESELLARVRKGEFDIALLPLTMTSSGPETLLTGFLSDEAANP
                     ARYQSAAFDGFARQGLAATTSQAQAAAWGAAERQLLSDAAVAPLAFQSSGFYLAPDLQ
                     GVVVSPFGPVIDLTGASRK"
     gene            complement(3210..4061)
                     /locus_tag="ACZPFK_00985"
     CDS             complement(3210..4061)
                     /locus_tag="ACZPFK_00985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR1404912.1"
                     /translation="MKTLKIAMVQRRACGPDVQKNTALALRCIAQAKAENADLVLFPE
                     CFLTAYWFPRICETLRPVEAIETDEEFVRWCESALEDDSPPLNQLRQAARALDIGVAA
                     TCFTRGKKYPRNTVFLIGRDGSVLLKYSKVHTCDFSLERYLESGDGFQVCRFDGITLG
                     AMICYDREYPESARELMLQGAEVILLPNDCDCMRPFRLRELSVEAMQNMVGIAMANPP
                     GESAGCSCAFHPMVWGREENSLVVADENFDGLVYAVFDIDEMRSYRAAEDLGKFRKAA
                     AYRHLSR"
     gene            complement(4242..6329)
                     /locus_tag="ACZPFK_00990"
     CDS             complement(4242..6329)
                     /locus_tag="ACZPFK_00990"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1404913.1"
                     /translation="MADFDLKERFGSHLFDDRVMRTRLPGNVYECLRAIRDEGREWDP
                     AVADVVAEVMKNWAMELGATHYVHWFSPMTGTNSGKHEAFIDGVDFGQPLLSFSGKML
                     SKGEPDASSFPSGGLRATFEARGYTTWDPTSPAFVLGTTLYIPTAFCAYTGEALDQKT
                     PVLRSMQALNKQALRVLRALGDKAVISVHPTVGAEQEYFLVDKELLDKRLDLKVCGHT
                     LMGARPPKGQELDDHYYGSISERVRAFMRELDEELWALGVPAKTEHNEAAPGQYELAS
                     VFASSNIACDHNQIMMELMRKVALRHGFACLLHEKPFAGVNGSGKHNNWSLSTDRGEN
                     LLKPGKDPAGNPRFLLFLAAVIEAVDRYADLLRLAATCPGNDDRLGGNEAPPAVVSIF
                     LGDPLVKVLDAVATSHEVQGGERKKLLTGVKSLPLVTLDDADRNRTSPFAFTGNKFEF
                     RMVGSSQSIGLVNAVINGVVADTLRGYADRLEAAADPQAELMQIVRQAWVEHSRIVFN
                     GNNYSEEWAAEARRRGLPCLDHMAAAARAFVDQKNVDMFARTKIFTPDECRSRYEVLL
                     ERYVKTTHIEALTMLEMVDRSVLPASIEFMGRIARSSYYAGQEGVSSRATVQLAKRLA
                     AGIDAMETARDRLAARVKSLKARDAQQEADVWYAIRTGEMARLRAACDRMEELVADSA
                     WPMPGYSDLLYDL"
     gene            7287..8687
                     /gene="murD"
                     /locus_tag="ACZPFK_00995"
     CDS             7287..8687
                     /gene="murD"
                     /locus_tag="ACZPFK_00995"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1404914.1"
                     /translation="MKTKAEAFFEGLAGKRVAFIGAGVSHRELLPLFAKRGAVVTLCD
                     KKGSVEEFGPLGEELRALGVRFSLGQGYLDGLRGQDMILRTPGFEYFTPALQQALAQG
                     AEVTSEMELFFELCPCKIYAVTGSDGKTTSTTLIAEMLRAEGKTVHLGGNLGRALLPV
                     VDTVRPGDVAVVELSSFQLISMHTSPDVALVTNVTPNHLDHHKDMAEYVDAKRNILLH
                     QRPGSKAVLGYENEITRAMARDVVGATYWFSRLNRVARGAFLAENGDLCFAENGVVTP
                     VLHRSEVKLRGLHNIENLLGAIATVAGEVRFETMARVARQFAGVEHRIEPVRLLHGVQ
                     WYNDSIASSPTRTIAGLRSFDQKIIIIAGGYDKHIPYEPLAPEILAHVKVLILMGDTG
                     PIIEKAVRECPGFDGSGLVIEHAGDMAEAVEIARRRAVAGDVVSLSPASASFDKYPNF
                     EVRGRDFKRLVNALPE"
     gene            8684..>9131
                     /locus_tag="ACZPFK_01000"
     CDS             8684..>9131
                     /locus_tag="ACZPFK_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404915.1"
                     /translation="MIARADAGDREVFFELLRRDLLFGGRIATAFDCWGGHPGACGFY
                     RVGRSAALLVQGGGALLCGAPEDDGAEELAAFLHFAGAGRLCSRRTCPPGWLRRELIE
                     MELRPAAAPAEGAPPGPALPGGARLERHPSLWRLRQSGLLHGADPDG"
BASE COUNT         1447 a         3062 c         3097 g         1525 t
ORIGIN      
        1 agcgggtggt ggacggggtg gtggaatccg ccgaggtggt caaggtggag gacgtggtcc
       61 ccgcccagga taccgtcatc atgcgctacg gcgcgggcgc gccggtctcc cagctggagg
      121 ggccggccgg ggtcactgtg gaaaacgggg tgcccagcag ctaccggcag gtgctcaccg
      181 gccgggcaac cgcctactcc gccagccgcg ggcgcggctc gtcggggctg ggcctctacg
      241 agggcaccgt ggcggtggac cccaacgtca tcccctacgg cagcctgctg tatatcacct
      301 ccaccgacgg ccggtttgtg tacggctacg ccgtcgccac cgacaccggc tcggcgatgg
      361 tcgagggcca cgctttggtg gacctgttct acgacacctt tgacgaggcc tatctgtccg
      421 ccgttcaaag cgtcaacgtc tatatcctgt aagtctgcaa aaaagggcgc ccggccgcgg
      481 aggctgggcg ccctttttta ttgcttcgca ataaaaaaac ttatcttgat ggccgcgttt
      541 ctcgccgcta cgcggcagcc gaaacggatg gccgaaaaaa tagccttaca gctatttttt
      601 attgcttcgc aataaaaaaa cttatcttga tagccccgtt tctcgccgct gtgcggcaac
      661 cgaaacggat ggccaaaaaa atagcctgcg gctatttttt attgctttgc aggaacattt
      721 tattttaagg cccgggcggg ggagcagcca gggagaccgc ccggggggct caaccgggaa
      781 accggccgat cggctgaaaa tacagcctgc ggctgttctg gatcgtttcg cactaaaacg
      841 gcttgtttta cgggccccgg cccggggaga ccggctcgag gagaccaacc ggaaaccggc
      901 ggggcggccg cccccatagc cgccgtgcgg ctgttctttt ggggcggttt acttgcggct
      961 ggcgcccgtc aggtcaatga ccgggccgaa agggctgacg acgaccccct ggaggtccgg
     1021 ggcgagataa aagccgctgc tctggaacgc cagcggcgcc acggcggcgt cgctgagcag
     1081 ctgccgctcg gcggcccccc aggcggcggc ctgcgcttgc gaggtggtcg ccgccagccc
     1141 ctggcgggca aagccgtcga acgcggcgct ttggtagcgg gccgggtttg cggcctcgtc
     1201 ggagagaaag ccggtcaaca gggtttccgg cccgctggag gtcatggtga gcgggagcag
     1261 ggcgatgtcg aactcgccct ttcgcacccg ggcgagcagc tcgctctccg gcagggcctg
     1321 caccgagaaa aaggccgaca ggctgcgctg ccagcgctgg ttgatgatcg ggtagagctc
     1381 gccccagggc tctgtctcgg ggatcagcac ctgcaggccg gagaggcgct cgacgcccag
     1441 ctcggacaac ccgagccggt agagggaata gctctgcgcc tcggccagcg agggcagcgc
     1501 cccgcccacc gcgtcccggt aaccgccgcc gccggtgagc cgggcgctgc ccggcaccag
     1561 gccgtccgcc gcgcgcagat ggcccaggcc cgccaggtcc tcccccgaca gctgcgccac
     1621 cgaggccagg gcctgccgga tggagaggtt ggagagggct tcgtcggcgc agttgaaaac
     1681 cagcgtccag accgtggtct caaaggtctg cgagggatag cccgccagct gggtctcctg
     1741 gtccgcggcg gcgagcgcgc cgtcctgcag accgtccgcc agccgcgcgg cggcggtgct
     1801 gccgccgtcg tcgggcacca ggcggatgcg gttgacgagg tcgccctcat ccccgcgcag
     1861 gcggcgcagg gtcaggccgt tggcctgcga ccagcccgtg ggctgaaagg cgccgttgcc
     1921 caggatcgcc tttaccgaga ggccgtaggc cccggcggtg gagtcgaaaa actcctcgtt
     1981 gcagggcatg gcgcccgcgg ttgccagctt ggcgggcagg ccgtcgtccg gggcggccag
     2041 cgcgatttcc agcacggtgt ccgacactgc gcgcaccccc agcgactcgg gccgggcggc
     2101 gccggccagc acctccgccg cgccgcggag ggagagaaag ctgcgggcgg agggcgcgcc
     2161 ggtggcgggg tccagcagcc gccgcagggc aaagacataa tcctttgccg tcaccggcac
     2221 cgtgatttcg cccccgtcgg taaagacgtc ctccgggtcc agcgtgaaag tgtagaccag
     2281 cccgtcggcg ctgatctcga ccccgcgggc ccggtcgggc accgcccggc cctcgccgtc
     2341 caggcgatag agcccggcga acatattctt caccgccgtg agctcggccg gggtggaggc
     2401 gagctgcggg tccagggtgg cgatggcgtc ggaggtgtaa tagatgaagt tgaccgcgtc
     2461 ctttttgcag ccggtcaaca ggcacaggca gagcgcgccc gccagcgcca gcgcggcgag
     2521 acgttttgtc aacgggaacc cctcctttga aagatgatgc ggcatcactg tgctcctgat
     2581 ttttacgttt ttatgcgcat ttgcccccct ctgtgggcgg cttgagattt taaggccgtg
     2641 ggcgggaacg gcccctgcgg ccgttctgcc cgccgtctgc ttcggccgcc gccttgcggg
     2701 ggctgcaagg ggggagggaa acacggcgga gcaagatcgt tcgtttttga ttgcgcagct
     2761 ttgatcgcac gacagcacaa aacaatttat cccggcggct cccggcccgt gggcgggcgc
     2821 cgaaaagatt gaaagccccg ccccgatcct gagagcggcc cgcggccgcc gccctctgaa
     2881 gcggcccaca ccgccatccc ctcaggcggc tcgcggccgc cggttcccct gcaagcggcg
     2941 ttctggaccg cgcggccccc ccctcagggg gcccgcaaaa ggcatcctat cagtagtaac
     3001 gcaggtctgc ccttaatgtt aacaaaaaga ttcttaaaaa acaatgaata agcacaaaag
     3061 aacgccgaaa aatttgtgaa aaagacgaaa attcagcaat agaaaaggca gggctgaaaa
     3121 aatcagccct gcctgaagtt tttgccgccc ccgggggcgc tggtcctgcc gcccgaaagg
     3181 ggaaacaggc gtggagcagc cggcccgggt cagcgggaca gatgccggta ggccgccgcc
     3241 tttctgaatt tgcccaggtc ctccgcggcc cgatagcttc tcatctcgtc gatgtcgaac
     3301 accgcgtaca ccagcccgtc aaaattttca tcggcgacga ccaggctgtt ctcctcgcgg
     3361 ccccacacca tggggtgaaa ggcgcaggag cagcccgcgc tttctcccgg cgggtttgcc
     3421 atggcgatgc ccaccatatt ctgcatggcc tccaccgaca gctcccgcag ccgaaacggg
     3481 cgcatgcagt cacagtcgtt gggcagcaaa atcacctccg cgccctgcag catcagctcc
     3541 cgggcgcttt cggggtattc ccggtcatag cagatcatgg ccccgagcgt gatcccgtca
     3601 aaacggcaga cctgaaaacc gtcgccgctt tccagatagc gttccaggct gaaatcgcag
     3661 gtgtgcacct tgctgtactt caaaaggacc gagccgtccc tgccgatgag gaaaacggtg
     3721 ttgcggggat attttttccc tctggtgaag caggtggccg ccacgccgat gtccagcgcc
     3781 ctggcggcct ggcggagctg gttcagaggg ggggaatcgt cctccagcgc actttcgcac
     3841 cagcggacga attcctcatc tgtttcaatc gcctcgaccg gccgcagggt ttcgcagatc
     3901 cgggggaacc agtacgcggt gaggaagcac tcggggaaga ggacgagatc ggcgttttcc
     3961 gcttttgcct gcgcgatgca gcgcagcgcc agcgcggtat tcttttgcac gtcgggcccg
     4021 cacgcccggc gctggaccat ggcgattttc aatgttttca tagctgtttt cctctttttc
     4081 agcgcagagg ggaggggtcc ggccgcccgg cttttgcttt tttctccgaa aagctcctcc
     4141 tcacggcggc cctgggtcaa aaagcccttt ccccgaccgg cccggccgcg ggtcgtcggc
     4201 gcggcgaaaa gcgtccggcg tccggggcct tactgcgggc cctacaggtc gtacagcagg
     4261 tcgctgtagc ccggcatcgg ccaggcgctg tcggccacca gctcttccat ccggtcgcag
     4321 gcggcgcgca gcctggccat ctccccggtg cggatcgcgt accagacgtc cgcctcctgc
     4381 tgggcgtccc gcgccttgag gctttttacc cgggcggcca gacggtcgcg ggcggtctcc
     4441 atggcgtcga tgcccgcggc cagccgtttg gcgagctgca cggtggcccg gctggacacc
     4501 ccctcctgcc cggcgtaata gctgctgcgg gcgatgcggc ccataaactc gattgaggcg
     4561 ggcagcacgc tgcggtccac catctccagc atggtcaggg cctcgatgtg ggtggtcttg
     4621 acataccgct ccagcagcac ctcatagcgg ctgcggcact cgtcgggggt gaagatcttg
     4681 gtgcgggcga acatgtccac gtttttctga tccacaaagg cccgggcagc ggcggccatg
     4741 tggtccaggc agggcaggcc gcgcctccgc gcctccgcgg cccactcctc cgagtagttg
     4801 ttgccgttga agacgatgcg gctgtgctct acccaggcct ggcgcacgat ctgcatcagc
     4861 tcggcctggg ggtcggcggc ggcctccagc cggtcggcgt agccgcgcag cgtgtcggcc
     4921 accacgccgt tgatcaccgc gttcaccagc ccgatgctct gggaggagcc caccatgcgg
     4981 aactcgaact tgttgcccgt gaaagcgaac ggcgaggtgc ggttgcggtc ggcgtcgtcc
     5041 agcgtcacca gcggcaggct cttgacgccg gtgagcagct ttttgcgctc gccgccctgc
     5101 acctcgtggc tggtggccac ggcgtccagc actttcacca gcgggtcgcc caaaaagatg
     5161 gagaccacgg cgggcggggc ctcgttgccg cccaggcggt cgtcgttgcc cgggcaggtg
     5221 gcggcaaggc gcagcagatc ggcatagcgg tccaccgcct cgatcaccgc ggccaaaaac
     5281 agcagaaagc gcgggttgcc cgccgggtcc ttgccgggct tgagcaggtt ttcgccccgg
     5341 tcggtggaga gggaccagtt gttgtgcttg ccgctgccgt tgaccccggc aaagggcttt
     5401 tcgtgcagca ggcaggcaaa gccgtggcgc agcgccacct tgcgcatcag ctccatcatg
     5461 atctggttgt ggtcgcaggc gatgttcgag ctggcgaaca cgctggccag ctcgtactgg
     5521 ccgggcgccg cctcgttgtg ctcggtcttg gcgggcacgc ccagggccca cagctcctcg
     5581 tccagctccc gcataaaggc gcgcacccgc tcgctgatgc tgccgtagta gtggtcgtcc
     5641 agctcctgcc ccttgggcgg ccgcgccccc atcagggtgt ggccgcagac cttcaggtcc
     5701 agccgcttgt cgagcagctc cttgtccaca agaaagtact cctgctcggc gcccacggtg
     5761 gggtgcaccg agatcaccgc cttgtcgccc agcgcccgca gcacccgcag cgcctgcttg
     5821 ttcagcgcct gcatgctgcg cagcaccggg gtcttctggt ccagcgcctc gccggtatag
     5881 gcgcaaaagg cggtggggat gtacagggtg gtgcccagca caaaggccgg ggaggtgggg
     5941 tcccaggtgg tgtagccccg cgcctcaaag gtggcgcgca gccccccgga ggggaagctg
     6001 gaggcgtccg gctcgccctt ggagagcatc ttgccggaaa agctcagcag cggctggccg
     6061 aaatccaccc cgtcgataaa ggcctcgtgc ttgccgctgt tggtgccggt catgggcgag
     6121 aaccagtgca catagtgggt ggcccccagc tccatggccc agttcttcat cacctcggcc
     6181 accacgtcgg caacggcggg gtcccactcc cgcccctcgt cgcggatggc gcgcaggcat
     6241 tcgtagacgt tgcccggcag ccgcgtgcgc atgacgcggt cgtcgaacag atggctgccg
     6301 aaccgctctt tcaggtcaaa atccgccaaa ggtacccctc ccgaaatttg tgtattttgc
     6361 tgaattttta tcgtgtccta ttgtaacacg tgacggcggc gcggagcaag aaaaaatttg
     6421 gagaaaacgg cgcgggagcg gctttttcat cggcggcccc gggcggggcc gccccggggc
     6481 cgcggccgca gcccggcggt ggggagaaaa gcgccgcccc gtcccggctg gccgccgcac
     6541 aagtgtggag cggcaggcag aactttgccc tcggtacggc ctcccggccg gccatccgcc
     6601 caggctgttc gctccgcacg gtggccgccc gctcccgcgc ccgccgcctc gccgcgcctc
     6661 tcgccgccgt ggtcacccgc ttctcgccgt gcctactccc tcctaaccgc agccgcccgc
     6721 tcttcaccgc gcccgcacgt ttctcaccgc gcccgcccgt ttctcaccgc ggccgcccgc
     6781 tcccgccgcg accgcccgcc gtcgggaccc ccaggcgccc ggctcctggc ggcacggcgg
     6841 ggtgcaatgt cgggctgaaa gcgaagagga agaccggcgc gggcccgatg ccgcgtgccg
     6901 ggcggctggg gcagattgac tggccgggcc cggaccggac tgtgcgggcg cggggccgcg
     6961 tgagagctcc ggctgcaaaa gcagcgggca tccccacagc gcccgcgcat cccccgccgc
     7021 gcctatccgc ttctccccgc ggcgttgggc gggcgatgcg gacgctcccc gcgcttcccc
     7081 ccggcgcatt ttgggcggct gcggggtcac aaaaaagccc ctgcggcggc gcggcggtcc
     7141 tccccgcaac tttccgcgcc taagccgggc ttgattggcc catcctttca tattataata
     7201 gtaaagctat cggccggcgc gcggcgcttt tgcggccgca ctcggccgcc gggccggacg
     7261 aaacggggaa aaggggtctt gcggagatga agacaaaagc ggaagctttt tttgaggggc
     7321 ttgcgggcaa gcgggtggcc tttatcgggg ccggggtctc ccaccgggag ctgctgcccc
     7381 tctttgcaaa gcggggggcg gtggtcaccc tctgcgacaa aaagggctcg gtggaggaat
     7441 tcggccccct gggcgaggag ctgcgcgccc tgggggtgcg cttctcgctg gggcagggct
     7501 atctcgacgg cctgcgcggg caggacatga tcctgcgcac gccgggcttt gagtatttta
     7561 ccccggcttt gcagcaggcc ctggcacagg gggccgaggt caccagtgag atggagctct
     7621 tcttcgagct ctgcccctgc aagatctatg cggtcaccgg ctctgacggc aagaccacca
     7681 gcaccaccct catcgccgag atgctgcggg ccgagggcaa aacggtgcat ctggggggca
     7741 acctgggccg ggcgctgctg ccggtggtgg acacggtgcg gcccggcgac gtggccgtgg
     7801 tggagctgtc cagctttcag ctgatctcga tgcacacctc cccggacgtg gccctggtca
     7861 ccaacgtcac cccgaaccat ctggaccacc acaaggacat ggcggagtac gtcgacgcca
     7921 agcgcaacat cctgctgcac cagcgccccg gcagcaaggc ggtgctgggc tatgaaaacg
     7981 agatcacccg cgccatggcc agggacgtgg tgggcgccac ttactggttc agccgcctca
     8041 accgggtggc gcggggcgcc tttctggcgg aaaacggcga cctctgcttt gccgagaacg
     8101 gggtggtgac cccggtgctc caccgcagcg aggtgaagct gcggggcctg cacaacatcg
     8161 aaaacctgct gggggccatc gccaccgtgg cgggcgaggt gcggtttgag accatggccc
     8221 gggtggcgcg gcagtttgcc ggcgtcgagc accgcatcga gccggtgcgg ctgctgcacg
     8281 gggtgcagtg gtacaacgac tccatcgcca gcagccccac ccgcaccatc gccgggctgc
     8341 gcagctttga ccagaaaatc atcatcatcg ccggcggcta tgacaagcac atcccctacg
     8401 agccgctggc gcccgagatc ctggcccacg tcaaggtgct gatcctgatg ggggacaccg
     8461 ggcccatcat cgaaaaggcg gtgcgggagt gccccggctt tgacgggagc ggcctggtca
     8521 tcgagcacgc gggggatatg gccgaggcgg tggagatcgc ccggcggcgc gccgtggcgg
     8581 gggacgtggt ctcgctctcg cccgcctccg ccagctttga caaatacccg aacttcgagg
     8641 tgcgggggcg ggacttcaag cggctggtca acgccctgcc cgaatgatcg cgcgggccga
     8701 tgcgggggat cgggaggttt tttttgagct gctgcggcgc gacctcctct tcggcggccg
     8761 catcgccacc gcgttcgact gctggggcgg ccaccccggt gcctgcggtt tttatcgggt
     8821 cggccgctcc gccgccctgc tggtgcaggg cggcggagcg ctgctctgcg gggcgcctga
     8881 agacgacggg gcggaggagc tggccgcttt tttgcacttt gccggagccg ggcggctgtg
     8941 ctcccgcagg acctgcccgc caggctggct gcggcgggag ctgatcgaga tggagctgcg
     9001 ccctgccgcc gcgcccgcgg aaggggctcc tccggggccc gccctgccgg gcggcgcgcg
     9061 gctggagcgg catccctcgc tgtggcggct gcggcagagc ggcctgctgc acggcgccga
     9121 cccggacggc t
//
LOCUS       DBMDYA010000020         9120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2071_length_9120_cov_1.676128, whole genome shotgun sequence.
ACCESSION   DBMDYA010000020 DBMDYA010000000
VERSION     DBMDYA010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2071_length_9120_cov_1.676128"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="ACZPFK_01005"
     CDS             <1..732
                     /locus_tag="ACZPFK_01005"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIR1404916.1"
                     /translation="AHYNCMLIDLDNTLLDFDAAEASALAATLAHFELPGDAQTIAEY
                     RTINEKLWREFERGGIKKERLLTLRFSKLLNHLGRQGSPAKINDYYLAELAKGAQILP
                     GADEFLADVEDYVTVGIITNGAQKAQMGRLQASGIGAFADGVFISEKLGVAKPDKRFV
                     NIALEKLGVGNRKKVLVVGDSLAADIKCGAAAGLDTCWCNFKEQENDTGIEPTYTVRG
                     FEELKAVILEQEELDHVGEKKKYQL"
     gene            701..1651
                     /locus_tag="ACZPFK_01010"
     CDS             701..1651
                     /locus_tag="ACZPFK_01010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1404917.1"
                     /translation="MSVKKRNISFNSANGEDTVVGYFYTDEAVRPHAVVQLSHGMCEY
                     IGRYDDFAAYLCARGYAVCGNDHLGHGATGDSGTDGYFAEHDGRKYVLEDLHTMNRLA
                     HEAFPELPLVLFGHSMGSFFARQYAALWPDTIDGLVICGTGGPNPLGGVGLALTRVLG
                     AVRGGKYRSKFINNLAFGAYLKRVPSPDTPYDWISRDKEIVTAYAQDPKCTFIFTVSA
                     FHELMAILRDVSSPAWAARLKKSMPVLMISGDMDPVGDYGEGVKKVFGWIKEAGVRDA
                     QLKLYPGARHEVLNETNRAEVYADVAAWLEDKFPARGAAE"
     gene            1792..2961
                     /locus_tag="ACZPFK_01015"
     CDS             1792..2961
                     /locus_tag="ACZPFK_01015"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1404918.1"
                     /translation="MDALSPFAQSLFGSALSMSGIEAAKMLVMWAIGGALIYLAIKKD
                     MEPTLLLPMGFGAILVNLPLSGVIDQGIDPVTGIAAEPGALSELFRAGIDNELFPLLL
                     FIGIGAMIDFGPLLQNPKLLLFGAAAQFGIFFTITAATLLGFSITDAASIGIIGAADG
                     PTSIFVGTYYGTKYIGAIIVAAYSYMALVPIIQPPCIKLVTTKKERCIRMPYEAKQTS
                     KTVRILFPIVVTVIAGLVAPRSVALVGFLMFGNLLRECGVLGSLSEAAQNVLANLITL
                     LLGITVATKMQAELFLTWQTLMILALGLVAFIFDTIGGVLFAKLINLFSKKKVNPMIG
                     ACGISAFPMSSRVVAKMALKEDPQNFILMQAAGVNVGGQIASVIAGGLILNLIAR"
     gene            2978..3100
                     /locus_tag="ACZPFK_01020"
     CDS             2978..3100
                     /locus_tag="ACZPFK_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HIR1404919.1"
                     /translation="MDMNAVTQSLNTMLFGMCGIFIVMGVISVAIALLNRLFKK"
     gene            3224..5314
                     /locus_tag="ACZPFK_01025"
     CDS             3224..5314
                     /locus_tag="ACZPFK_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="G protein-coupled receptor family protein"
                     /protein_id="HIR1404920.1"
                     /translation="MEMEKIWRQAYNGLQRTKKMLLFFCLGSYLLTVLLSGGTLMGAF
                     RFWLCVLGYLYLPGLLLVRVAGLDRDLPHARSPLAILFGTGFLCVLYCFCMRLGLLWA
                     LRLLPPLLAICWLLLCWNPVETQAGLKKVWRSGPALFYGLLVAGLIALFAFSVVVKNA
                     RPSAVGAILPNQDLLWNIGNANSFKLAFPPQDIRFSQVRLTYHYLTEMVEGILSLVSG
                     LPAYDVVAFYMGPAVLLALVCCLFALGNLFYRGNRDKTAAFVFGLFLFNCASMFAALL
                     DGRGLFWNYNLSYLVTNLNAQGTALIFASLFFLLFTGMARKDFRVSWRCLAAFLGSFV
                     LLCFAKGPVAAVTACSFAITMVFVLFRRPHYGKALVSLAGVLGIFVTVYTTFYASRVN
                     TSMRLGDRTFQMSAFSHYFNAVMLHNRYLWYLSIPLFALLILFCMQPLQLPLYLRGLW
                     DDVRGLFHLPAERLLINGAACGGAIAYFLFWHPSYSQIYFVLFAIFCINLLAADRVGR
                     PAANPFKAMMVLLGGVGLLTTLVLCVNFVGSGGRQLLRNLDIIPKYPYPAVVCAEDEA
                     AMEWLSENAAPNDLFATNRIDAQPGTGEGVSCIYTALSGRQAYMEGYTYAVTNMGVAE
                     AAVAEKKAVNAAFFSANTSPSEIVRLARENGVRYLVVSLQFPGDTIQLVQFPLVYENS
                     KVQIYRVENTPGEE"
     gene            5331..7175
                     /locus_tag="ACZPFK_01030"
     CDS             5331..7175
                     /locus_tag="ACZPFK_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1404921.1"
                     /translation="MLITLQKTGKSFGAERVLRDVTAAVDRQDRIGIIGENGTGKTTL
                     LRIITGELQPDEGEVTYSHNLTWGYLEQNAALDTARTVWQEMEDAYAPSLAAMAELEE
                     VNRALALRPGDASLLERHAAASAVIDAQDAYNRDVQIKKVLAGGERTRLQLAKLLLQN
                     PDVLILDEPTNHLDFETLEWLEAYLKTYGGAILTVSHDRYFLDAVTNRTWELEQNTIT
                     CYKGNYSAYLPQKELAVAVQQKQHDADVARAKKLQDYVDKNLVRASTTKMAQSRRRQL
                     EKMEITEQPHRAGYELKFRFEYDLDPYEDVLTTKKLCVSAGGSRLVSGLDLNVLRGDR
                     LVIAGPNGTGKSTLLRVLAGKLRPDSGAVRLGSGVKLSTFEQQQRRAGGRVIDAIWNK
                     YPRMTELEVRSHLARLGFKGEDVFKEVPTLSGGELSRLRFAEILLERPNLLFLDEPTN
                     HLDIYMRESLSQALAAFTGTLLLVTHDRYLMNSLACPILYLENGGATFYAGYRQLLAH
                     TTMEAPQGAEQRKAAAAGGGKTQRSQKEERRLKAEVRSRAKELEREIEALGADILEME
                     ENLSLPEIASDHLRLTELCDALDDARFRQNELYAEWERLLEEHGEYLE"
     gene            7334..7873
                     /locus_tag="ACZPFK_01035"
     CDS             7334..7873
                     /locus_tag="ACZPFK_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1404922.1"
                     /translation="MSSIDLIVLGMVMERPQSAYELQKDVKAHHLSRWTRVSVPSIYK
                     KVLQLRAAGYLEGGAARGGRSADKTVYTITEKGRRYFRQLMEECAGGQVAFLLDLNVM
                     VTNLNKLEKEEGLRLLGLLRQSLTASAHSNRLYAQQFKDIPLVGRTVFEQQELLYAAL
                     ERWLDDFERRFAASQGAAG"
     gene            7870..8286
                     /locus_tag="ACZPFK_01040"
     CDS             7870..8286
                     /locus_tag="ACZPFK_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HIR1404923.1"
                     /translation="MTARGSLTVLFDPPFWVGLCERSEQGRYEVCKVVFGAQPQEAEV
                     YAFVLHCWDKLRFSPALPEEEREAKCASPKRIQRQISASLHRRGMSTKAQQALQRQRE
                     EGKSARRERVRRQRQEEQEQRYALRAEKKKTKHRGR"
     gene            complement(8387..>9120)
                     /locus_tag="ACZPFK_01045"
     CDS             complement(8387..>9120)
                     /locus_tag="ACZPFK_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1404924.1"
                     /translation="EGPWMERMAQAFGGDALFLDPTGTLREHADEYLFYRTDHHWTTS
                     GAYYAYLQYAGAVGKAGAAAAALVPTQVEDFFGTLYSKTKLYNAQADLLAYYPQLDGR
                     MTIVQLLDQADREAVQQAYQAGQMTEEAYAQVSDRVVDLYETDKLSTRDKYAMFLYGN
                     NGFSRIEGEGEGKVLVVKDSYANCFVPFLTADYAQIDVVDLRSLKGATVDSLIAENGY
                     DDVLVLYNFQSFSSDTNLVLLNMGG"
BASE COUNT         1749 a         2596 c         2920 g         1855 t
ORIGIN      
        1 gcgcattaca actgtatgct catcgacctg gacaacacgc tgctcgattt tgacgccgcg
       61 gaggcgtcag ccctcgcggc cacactggcg cattttgagc tgccgggcga cgcgcagacc
      121 atcgcggaat accgcacgat caacgaaaag ctctggcggg aattcgagcg cggcggcatc
      181 aaaaaagagc ggctgctgac gctgcgtttt tccaagctgc tgaaccatct gggcaggcag
      241 ggcagcccgg cgaagatcaa cgactactat ctcgcagagc tggccaaggg ggcgcagatc
      301 ctgcccggcg cggatgaatt cctcgccgat gtggaagact atgtgacggt gggcatcatc
      361 accaacggcg cccagaaagc gcagatgggg cggctgcagg cctccggcat cggggcgttc
      421 gccgacgggg tcttcatctc ggaaaagctg ggggtggcaa agcccgacaa gcgctttgtc
      481 aatatcgcgc tggaaaaatt gggcgtcggc aaccgcaaaa aagtgctggt ggtgggcgac
      541 agtctggcgg cggacatcaa atgcggggct gcggcggggc tggacacctg ctggtgcaat
      601 ttcaaggaac aggagaatga cacggggatc gagcccacct atacggtgcg gggctttgag
      661 gagctcaaag cggtgattct ggaacaggag gaattggacc atgtcggtga aaaaaagaaa
      721 tatcagcttt aattctgcaa acggagagga cacggtcgtc ggctattttt acaccgacga
      781 agcggtgagg ccccacgccg tggtgcagct gtcccacggc atgtgcgagt acatcggccg
      841 ctacgacgac tttgcggcct acctctgcgc ccggggctac gcggtgtgcg gcaacgacca
      901 cctggggcac ggggccaccg gcgacagcgg caccgacggc tattttgccg agcacgacgg
      961 gcgcaaatat gtgctggagg atctgcacac catgaaccgc cttgcacacg aggcgttccc
     1021 cgaactgccg ctggtgctgt tcgggcacag catgggcagc ttttttgcgc ggcagtatgc
     1081 tgccttgtgg cccgacacca tcgacggtct ggtgatctgc ggcacgggcg gccccaaccc
     1141 gctgggtggg gtggggttgg cgctgacccg cgtgctgggc gcggtgcgcg gcggaaaata
     1201 ccgctcaaaa ttcatcaaca acctggcctt tggggcctat ctcaagcgcg tcccctcgcc
     1261 cgatacgccc tatgactgga tcagccggga caaagagatc gtgaccgctt acgcgcagga
     1321 ccccaagtgc acctttattt ttaccgtcag cgcctttcat gagctgatgg cgatcctgcg
     1381 cgacgtgagc agccccgcct gggcggcaag gctgaaaaag tccatgccgg tgctgatgat
     1441 ctcgggcgac atggacccgg tgggggacta cggcgaaggg gtcaaaaaag tgtttggctg
     1501 gatcaaagag gcgggtgtcc gggacgcgca gctcaagctc tatcccgggg cccggcacga
     1561 ggtgctcaat gagacgaacc gcgccgaggt ctatgccgat gtggccgcct ggctggagga
     1621 taagttcccg gcccgcgggg cggcggagtg acctgcccgg caggcgcggc acgaatgatt
     1681 ttacaaggat tttacctttg tccctttcca aaagggcggc ggacatggta agatagcctt
     1741 actgtgccca aggggcgtca gacaagttga aaacgaaacg gagaatcgag aatggatgca
     1801 ctttccccgt tcgcgcagtc gttgttcggc agcgcgctct ccatgagcgg catagaggcc
     1861 gcaaagatgc tggtcatgtg ggccatcggc ggcgcgctga tctatctggc gatcaaaaag
     1921 gacatggagc ccacgctgct gctgcccatg ggcttcggcg ccatcctggt caacctgccg
     1981 ctgtccggcg tgatcgacca gggcatcgac cccgtcactg gcatcgccgc cgagccgggg
     2041 gcgctcagtg agctgttccg cgcgggcatc gacaacgagt tgttcccatt gctgctcttc
     2101 atcggcatcg gcgcgatgat cgattttggc ccgctgctgc aaaatcccaa gctgctgctg
     2161 tttggggccg cagcgcagtt cggcatcttc ttcaccatca ccgcggccac cctgctgggc
     2221 ttttccatca ccgacgcggc ctccatcggc atcatcggcg cggcggacgg ccccacctcg
     2281 atctttgtcg gcacctatta cggcacaaag tatatcggcg ccatcattgt ggccgcctac
     2341 agctatatgg cgctggtgcc catcatacag ccgccctgca tcaagctggt caccaccaaa
     2401 aaagagcgct gcatccgcat gccttatgag gcgaagcaga ccagcaagac ggtgcgcatc
     2461 ctgttcccga tcgtggtcac cgtgatcgcg ggcctggtgg cgccccgcag cgtggcgctg
     2521 gtgggctttc tgatgttcgg caacctgctg cgggagtgcg gcgtgctggg cagcctctct
     2581 gaggcggcgc agaacgtgct ggcaaacctg atcacgctgc tgctgggcat cacagtcgcc
     2641 accaagatgc aggcggagct ctttctcacc tggcaaaccc tgatgatcct ggctctcggc
     2701 ctggtggcgt ttatcttcga caccatcggc ggcgtgctgt ttgccaagct catcaacctg
     2761 ttcagcaaga aaaaggtcaa ccccatgatc ggcgcctgcg gtatctcggc gttccccatg
     2821 agcagccgcg ttgtggcaaa gatggctctc aaagaagacc cgcagaactt tatccttatg
     2881 caggcggcgg gcgtcaatgt gggcggccag atcgcctcgg tcatcgcggg cggcctgatc
     2941 ctcaacctca tcgcgcgcta gggagggaga aaacgagatg gatatgaacg cagttacaca
     3001 gtcgctgaac accatgctct tcggcatgtg cggcattttt atcgtgatgg gagtcatcag
     3061 cgtggccatc gcgctgctca accgcctgtt caaaaagtaa acggaagggc cctttgcaaa
     3121 acagccgcta tgtgataaaa tcagaagaga ccgccgcgcc caggcccgtg gatggcttag
     3181 gtcgcggcgg ttttgtttgt attgaaagaa aaaggagggg agcatggaga tggaaaaaat
     3241 atggagacaa gcctacaatg gcttgcagcg cacgaaaaaa atgctcctct ttttttgtct
     3301 gggctcttat cttttgacag tactgctctc cggcggcacc ttgatggggg cttttcgctt
     3361 ttggctctgc gtgttgggct acctttatct ccctggcctg ctgctggtgc gggtcgcagg
     3421 gctggatcgg gatttgccgc acgcgcgctc gccgctcgcc atcctgtttg gcaccggctt
     3481 tctctgtgta ttatactgtt tttgtatgcg gttggggctt ctgtgggcgc tgcgcctgct
     3541 gcccccgctg ctggccatct gttggctgct tttatgctgg aaccctgttg agacgcaggc
     3601 agggctgaaa aaagtatggc gcagcggccc agcgcttttc tacggccttt tagtcgccgg
     3661 attaatcgct ctgtttgctt tttccgtggt ggtgaaaaac gcccgtccat cggcagtggg
     3721 tgccatcctg cccaaccagg atctgctgtg gaacatcggc aatgccaatt ctttcaaact
     3781 ggcgtttccg ccgcaagata tccgcttttc acaggtgcgg ctcacctatc actatctcac
     3841 cgagatggtg gaggggattc tctcgttggt gagcggtctt cccgcttatg acgtggtggc
     3901 gttttatatg ggccctgccg tgctcctggc tttggtctgt tgcctgttcg cgcttggaaa
     3961 tcttttttac agggggaaca gggataagac cgcggcattt gtcttcgggc tctttctgtt
     4021 caactgcgcc agcatgttcg cggcgctgct cgatggccgc gggctttttt ggaactataa
     4081 tctgagctat cttgtaacca atctaaatgc gcagggcaca gcgctgattt ttgcttctct
     4141 cttttttctt ctctttaccg gtatggcacg caaagacttt cgggtctcct ggcgctgtct
     4201 cgctgccttt ttgggcagtt ttgtcctgct ctgttttgcc aaaggcccgg tggcggctgt
     4261 cacggcctgc agttttgcca tcaccatggt ctttgttctt tttcgccgtc ctcattacgg
     4321 caaagctctg gtttccctgg caggggtatt ggggatattc gtaacagtat ataccacttt
     4381 ctacgcctct agggttaaca ccagtatgag gctgggggac aggactttcc agatgagcgc
     4441 gttttctcat tacttcaacg ccgtgatgct gcataaccgc tatctctggt atctgtcgat
     4501 tccgcttttt gcgttgctga tcctgttctg tatgcagccg ttgcagctcc cgctctatct
     4561 gcgggggctt tgggatgatg tgcgcggact ttttcatctg cctgcagagc ggctgttgat
     4621 aaatggtgcg gcctgcggcg gcgcgattgc ctatttcctt ttctggcatc cctcttacag
     4681 tcagatctat tttgtcctgt tcgccatttt ttgtatcaac ttattggcgg ccgatcgagt
     4741 aggtcgcccc gccgctaacc cttttaaggc gatgatggtg ctgttggggg gcgtggggct
     4801 gttgactaca ctggtgctct gcgtgaattt tgtaggcagc ggtgggcggc agttactgcg
     4861 caacctggat atcatcccca agtaccccta ccctgcagtt gtctgcgcag aagacgaggc
     4921 agcgatggaa tggctgtcgg agaacgccgc gccgaatgac ctgtttgcca ccaaccgcat
     4981 cgacgcccag ccggggactg gggagggggt ctcatgcatt tacaccgcac tttccggcag
     5041 gcaggcgtat atggagggat atacttatgc cgtcaccaat atgggggtag cggaagcggc
     5101 ggtggcggag aaaaaagcgg taaacgcagc ttttttttcc gcgaatacct ctccgtcgga
     5161 gatcgttcgt cttgcccgtg agaacggggt gcgttatttg gtggtttccc tccaatttcc
     5221 gggcgatacg attcagctgg ttcagtttcc gctggtgtac gaaaattcga aagtgcagat
     5281 ttaccgggtg gaaaataccc caggcgagga atagataaag gaatcgattt atgcttatca
     5341 cattacagaa aacaggaaaa agctttggcg ccgagcgggt gctgcgggat gtgacggccg
     5401 ctgtcgaccg gcaggatcgc atcggtatta tcggcgagaa cggcacgggc aagaccaccc
     5461 tgctgcgcat catcaccggc gagctgcagc ccgacgaggg ggaggtcacc tacagccaca
     5521 acctgacctg gggctatctg gagcagaatg ccgcgctgga caccgcgcgc accgtctggc
     5581 aggagatgga ggacgcctac gcgccctctc ttgccgctat ggcggagctg gaagaggtga
     5641 accgcgccct ggcgctgcgc cccggcgacg cctccctgct ggagcggcac gccgcggcca
     5701 gcgcggtcat cgacgcgcag gacgcttaca accgggatgt gcagatcaaa aaggtgctgg
     5761 cggggggcga gcgcacccgg ctgcagctgg caaagctgct gctgcaaaac ccggatgtgc
     5821 tcatcttgga cgagcccacc aaccacctgg actttgagac gctggagtgg ctggaggcct
     5881 atctgaagac ctatggaggc gcgatcctca ccgtgtccca cgaccggtat tttttggacg
     5941 ccgtcaccaa ccgcacctgg gagctggagc agaacaccat cacctgctat aagggcaact
     6001 attcggccta tctgccgcag aaagagctgg cggtggcggt gcagcaaaag cagcacgacg
     6061 ccgatgtggc ccgggcaaaa aagctgcagg actatgtgga caaaaacctg gtgcgggcca
     6121 gcaccaccaa gatggcccag agccgccgcc ggcagctgga aaagatggag atcaccgagc
     6181 agccccatcg cgcggggtac gagctgaaat tccgctttga gtacgatctc gacccgtacg
     6241 aggacgtgct caccaccaag aagctctgtg tttccgcggg cggcagccgc ctggtgagcg
     6301 ggttggatct gaacgtgctg cggggcgacc ggctggtcat tgccgggccc aacggcaccg
     6361 gcaaatccac gctgctgcgg gtgctggcgg gcaagctgcg gcccgacagc ggcgcggtgc
     6421 ggctgggcag cggggtcaag ctgtccacct ttgaacagca gcagcgccgc gcgggcgggc
     6481 gggtcatcga cgctatctgg aacaaatatc cccgcatgac cgagctggag gtgcgcagcc
     6541 atctggcacg gctgggcttc aagggggagg acgtcttcaa agaggtgccg accctttcgg
     6601 gcggggagct gtcgcggctg cgctttgccg agattttgct cgagcggccc aacctgcttt
     6661 ttctggatga gccgaccaac catctggaca tctatatgcg cgagagcctg agccaggcgc
     6721 tggccgcgtt taccggcacc ctgctgctcg tcacccatga ccgctatctg atgaacagtc
     6781 tcgcctgccc tatcctctat ctggaaaacg gcggcgcgac cttttacgcc gggtaccggc
     6841 agctgttggc ccacaccacc atggaggccc cccagggggc ggaacagcgc aaagcggccg
     6901 cggcgggcgg gggaaagacc cagcgcagcc agaaggagga gcgtcgcctg aaagcggagg
     6961 tgcgcagccg cgccaaagag ctggagcggg agatcgaggc cctgggggcg gatatcctgg
     7021 agatggagga gaacctctcg ctgccggaga tcgccagcga ccatctgcgc ctgaccgagc
     7081 tgtgcgacgc gctggacgac gcgcgctttc gccagaacga gctctatgcg gagtgggagc
     7141 gcctgctgga agagcatggc gagtacctgg aataaaaagt gaaaagagcg gaggcgcgcc
     7201 cggcgtttgc cccgctcttt ttgttttgtg ggaaaagacc ggccgctgcc cggaggaccg
     7261 cgcggtaggg gatagcgctt gacttatact ataatttata gtactataaa ttatagtata
     7321 aggaggtgag caaatgtcct cgattgattt gattgtcctg ggcatggtga tggagcggcc
     7381 gcaaagcgcc tatgagcttc aaaaggatgt aaaggcgcac catctgtcgc gctggacccg
     7441 ggtgagcgtg ccgtccatct acaaaaaggt gctgcagctg cgggctgcgg gctatctgga
     7501 gggcggcgcc gcccggggcg ggcgctcggc ggacaaaacg gtttatacca tcaccgaaaa
     7561 gggccggcgc tatttccggc agctcatgga ggagtgcgcc ggcgggcagg tcgcctttct
     7621 gctggatctg aatgtgatgg tgaccaacct caacaagctg gaaaaggagg aggggcttcg
     7681 gctgttgggg ctgctgcggc agagcctcac ggcctcagct cacagcaacc gcctctatgc
     7741 gcagcagttc aaggacatcc cgctggtggg gcggacggtt tttgaacagc aggagctgct
     7801 ttacgcggcg ctggagcgct ggctggatga ttttgagcgc cgctttgcgg cctcgcaggg
     7861 ggccgcggga tgacggcgcg gggcagcttg acggtgctgt ttgatccccc tttttgggtc
     7921 ggcctctgcg agcgcagtga gcagggccgc tacgaggtgt gcaaggtcgt tttcggcgcc
     7981 cagccgcaag aggcggaggt gtacgccttt gtgctgcatt gctgggacaa actgagattc
     8041 agcccggctt tgccggagga ggagcgcgag gcaaagtgcg ccagccccaa gcgcatacag
     8101 cggcagatca gcgccagcct gcaccggcgg gggatgagca ccaaggcgca gcaggccctg
     8161 cagcggcaga gggaagaggg caagagcgcc cggcgggaga gagtgcggcg gcagcggcag
     8221 gaggagcagg agcagcgcta tgcgctgcgg gcggagaaga agaaaacaaa gcaccgcggg
     8281 cggtagaaac ggcgcctgcg gagagggcgc ggcaacgcag agagggccgg aagcatattc
     8341 gcttccggcc ctctctgtga tggggtttgg cgcgttcaaa agcgcttcag ccgcccatat
     8401 tgagcagcac caggttggta tcgctggaaa agctctgaaa attgtacagc accagcacgt
     8461 cgtcgtagcc gttttcggcg atcaggctgt ccactgtggc ccccttgagg ctgcgcaggt
     8521 ccaccacgtc gatctgggcg tagtctgcgg tgagaaaagg cacaaagcag ttggcgtagc
     8581 tgtccttgac gaccagcacc ttgccctcgc cctccccctc gatgcgggag aagccgttgt
     8641 tgccgtacag gaacatggcg tatttgtccc gcgtggagag cttgtcggtc tcatacaggt
     8701 ccaccacccg gtccgagacc tgggcatagg cctcctcggt catctgcccc gcctgatagg
     8761 cctgctgcac cgcttcccgg tcggcctgat ccagcagctg cacgatggtc atccggccat
     8821 ccagctgcgg gtaataggcg agcaggtctg cctgcgcgtt gtagagcttg gtcttgctgt
     8881 acagggtgcc gaaaaagtcc tccacctggg tggggacaag cgcggcggcc gcggcccccg
     8941 ccttgcccac cgcgccggca tattggaggt aggcgtaata ggcgcccgag gtggtccaat
     9001 ggtggtcggt gcggtaaaag agatattcgt ccgcgtgctc gcgcagtgtg ccggtggggt
     9061 cgagaaagag cgcgtcgccg ccaaaggcct gcgccatccg ctccatccag gggccctcct
//
LOCUS       DBMDYA010000021         9021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2095_length_9021_cov_1.571525, whole genome shotgun sequence.
ACCESSION   DBMDYA010000021 DBMDYA010000000
VERSION     DBMDYA010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_9021_cov_1.571525"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="ACZPFK_01050"
     CDS             <1..292
                     /locus_tag="ACZPFK_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1404925.1"
                     /translation="ADFAEQYPDQLAAFLQEYQASTEWVNANVPEAAQLVEKFDIVKA
                     AVAEKAIPYCNITYLAGDEMKTAMAGYLQVLFDQNEKSVGDKLPGDDFYYAV"
     gene            327..>838
                     /locus_tag="ACZPFK_01055"
     CDS             327..>838
                     /locus_tag="ACZPFK_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404926.1"
                     /translation="MLLALLVWQLAAASLSSSLLLVSPLRVLERLCGLVREPQFYAAV
                     GFSFVRIVAGFLLGLGCGTLLAVAASRWHLAEVLLWPYVTVIKTTPVASFIILCLIWL
                     DSSSLPVFISFLMVLPIVYANMLEGIRQTDQKLLEMAKLFRVGAGRRLLYIYLPQLRP
                     YLITACSVALG"
     assembly_gap    839..848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <849..1062
                     /locus_tag="ACZPFK_01060"
     CDS             <849..1062
                     /locus_tag="ACZPFK_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404927.1"
                     /translation="ALGLSWKAGIAAEVIGIPDGSIGERLYEAKVYLNSADLFAWTVV
                     IIAVSIAFEKLFLWLLRAGYRRLEQM"
     gene            1093..1677
                     /locus_tag="ACZPFK_01065"
     CDS             1093..1677
                     /locus_tag="ACZPFK_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1404928.1"
                     /translation="MEDIIVQGLCKSFDGAAALRNFSARFPAGRITCVMGRSGCGKTT
                     LLNILMGFLSADAGTVAGVPARLAAVFQEDRLLEPFSAASNLAFVRPKASPALIGEHL
                     AAVGLAQAASQPVRELSGGMRRRVAIARAVLAGGELLILDEPFKGLDEATRRAVASYL
                     RRYTAGATVILVTHDEDEVGLMDGGLIRMEPPCA"
     gene            1834..2814
                     /locus_tag="ACZPFK_01070"
     CDS             1834..2814
                     /locus_tag="ACZPFK_01070"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR1404929.1"
                     /translation="MKIETRAEILDILKLPQREYLAAVAPEAKRVHREENGGRLTATA
                     MLGYSNVCKNQCLYCGMRAANSAVPRYRIEPQDVLASLAAAHEAGFRRVFLVSGEDPK
                     YGFERLLQIVAGAKALGMGWVSLACGEYERSQYEELRAAGADEYVVKFEMSDRETFDR
                     LNPSTSYDKRMNAIRWVKESGLLLASGNIVDYPGQTLEQLADDILLMKELEISWAPVI
                     PYLPAQNTPLAAEGGPGSRELNLKEISILRLMMPQVNITAQQPGENLADGLASDEGNL
                     AALAAGANFLFADLLPTAKAQAFRVVDNRITLGLDHIRDMARRSGMEFTF"
     gene            3369..3944
                     /locus_tag="ACZPFK_01075"
     CDS             3369..3944
                     /locus_tag="ACZPFK_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404930.1"
                     /translation="MKKLNLLALIVSKFLEILHWLAAALMLCLLALCLAAPNWLSGRL
                     AQGAQRFGRELTTYGFTLTVAGADGSVNLAAVALFTVGAIFILSLMAMVFRNVYLIFR
                     TAKGKTWFAKGDTPFQKDIVRMVREIGIFYLAVPIVSLLMSVVAVLVLGADAAEPSVN
                     FEGVVTGILLLCLSQVFAYGAQLERDVDGLL"
     gene            3960..4232
                     /locus_tag="ACZPFK_01080"
     CDS             3960..4232
                     /locus_tag="ACZPFK_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404931.1"
                     /translation="MGKIVLRLDRMMVERKMSLNELAQRVGISNVNLSKIKNNRVTAI
                     RFSTLAAICEVLDCEAGDILEFQRGAPAEADNPEKGILTAREKGGA"
     gene            complement(4470..5393)
                     /gene="trxB"
                     /locus_tag="ACZPFK_01085"
     CDS             complement(4470..5393)
                     /gene="trxB"
                     /locus_tag="ACZPFK_01085"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR1404932.1"
                     /translation="MGIKKVDVAVLGGGPGGYTAALYCARAGFSVVVIEKMAPGGQMA
                     TTGQVDNYPGFDEGIDGFELGERMQRGAERFGAETLFADVTAVELAGDIKRIVTTDGE
                     IGARAVVLSTGASPRELGLPEERELRGRGVAYCATCDGMLYKGKTVVVAGGGNSAAAD
                     ALHLAKLCKKVILVHRRDALRASQVYLTPLKESGVEFLWNSRITGILHGGQVTGVRVA
                     DVKTGAEREVPCDGLFVAVGRVPDTALFAGQVRLEEHGYVDADETTRTNLPGVFAVGD
                     LRSKPLRQIVTAASDGAVASHFIEEYLMQQG"
     gene            complement(5410..6159)
                     /locus_tag="ACZPFK_01090"
     CDS             complement(5410..6159)
                     /locus_tag="ACZPFK_01090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1404933.1"
                     /translation="MRLEGKVTVITGASRGIGYAVARAFAREGARVALCGSRLSSAER
                     AAQQLLAELPRAELLPLGVDVADTEAVRAMVAAVMRRWGRVDVLVNNAGVTSAKQIFD
                     MTDEDFDAVLRVNLGGAFKCTREVAPIMRRQGGGSIVNTSSMVGTYGGKMQTAYSSSK
                     FAVNGLTKSCAKELGPYHIRVNAVAPGVVGTDMVAQSVDERMMAGLKAMTPLGRMAQP
                     EELAGAYVYLASDEASFTTGTILHVDGGIVM"
     gene            complement(6256..6582)
                     /locus_tag="ACZPFK_01095"
     CDS             complement(6256..6582)
                     /locus_tag="ACZPFK_01095"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1404934.1"
                     /translation="MMIRKIDGAGFEQEVLRSEKPVAVDFMADWCGYCKRLSPALERV
                     AQKAGQAAEVVRVDIDESGELARRYQVDTIPTLILFSGGKEAARTVNPPSQAAVENWL
                     RENGAL"
     gene            complement(6637..7071)
                     /locus_tag="ACZPFK_01100"
     CDS             complement(6637..7071)
                     /locus_tag="ACZPFK_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1404935.1"
                     /translation="MRWTGLPVLALGAALLFTACAGEGQPSASQGGSTAGSKEAYHKI
                     SAEEAKEMMDAGGVTVVDVRREDEYKESHIPGAVLVPVESIGDQPPAALPEKEAVLLI
                     HCRTGVRSKRAAEKLVDLGYQNVYDFGGIVDWPYETESGEAG"
     gene            complement(7743..8321)
                     /locus_tag="ACZPFK_01105"
     CDS             complement(7743..8321)
                     /locus_tag="ACZPFK_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1404936.1"
                     /translation="MDQQKTGALIRRLRLEKGLTQRALAEAVGVGDKAVSKWERGAGC
                     PDVGTLGALAEALGTGVDRLLSGSLDANDTEGGNMKRIQFYICPSCGNLLTATGAAEL
                     TCCGRRLEPLRARCADEGHRPVVEQVEDEDYITFAHDMEKEHYIAFVASVGYDRVSLV
                     RLYPEQGAELRMPRVRRGKLYWYCSKHGLFTQ"
     gene            complement(8579..>9021)
                     /locus_tag="ACZPFK_01110"
     CDS             complement(8579..>9021)
                     /locus_tag="ACZPFK_01110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1404937.1"
                     /translation="LQAALPYEDRIFDLVFCNQVLMDLAEIEGTMHEFSRVIKPAGLL
                     WLSIVHPAFFIGDWQRDENGIATSKLVQGYLRPRAQLNEFWGSSLHYHRPVSFYLNLA
                     AESGFSLVRLTEPEAYDPAVNTSQLPLFLCAEFRRMPCSAQNKQ"
BASE COUNT         1677 a         2926 c         2663 g         1745 t
ORIGIN      
        1 cgcggacttt gccgagcagt atcccgatca gctggcggcg tttctgcagg agtaccaagc
       61 gtccaccgag tgggtcaacg ccaatgtccc cgaggctgcc cagctggtgg aaaagttcga
      121 catcgtcaag gctgccgtgg cggaaaaggc gatcccctac tgcaatatca cctatcttgc
      181 cggcgatgag atgaagaccg ccatggcggg ttacctgcag gtgctgtttg accagaacga
      241 gaagtcggtg ggcgacaagc tgccgggcga cgacttctac tatgccgtct aaaccgccaa
      301 gggcccgcgg ggagcgcgcc gccgccgtgc tgctggccct gctggtctgg cagcttgcgg
      361 ccgcgtcgct gtcaagctcg ctgctgctgg tgtcccccct gcgggtgctg gagcgcctgt
      421 gcgggctggt gcgcgaaccc cagttctatg cggcggtcgg tttttctttc gtgcgcatcg
      481 tggccggctt tctgctgggc ctcggctgcg gcaccctgct ggcggtggcg gccagccgct
      541 ggcatctggc cgaggtgctg ctgtggccct atgtcacggt catcaagacg acgccggtgg
      601 cctctttcat catcctctgc ctgatctggc tggattcctc cagcctgccg gtgttcatct
      661 cgtttctgat ggtgctgccc atcgtctacg ccaacatgct ggagggcatc cgccagaccg
      721 accaaaagct gctggaaatg gcaaagctgt tccgcgtcgg ggccgggcgc aggctgctct
      781 acatctatct gccgcagctg cgcccttatc tcatcacggc ctgcagcgtg gcgctgggnn
      841 nnnnnnnngg cgctgggcct gtcctggaaa gcggggatcg cggccgaggt catcggcatc
      901 ccggacggct ccatcggcga gcggctctac gaggcgaaag tctatctcaa ctccgccgac
      961 ctgttcgcct ggacggtggt gatcatcgcg gtcagcatcg cgttcgaaaa attgttttta
     1021 tggctgctgc gggcgggcta tcgccgcctg gagcagatgt gacccgcccg gcccggagag
     1081 gaggtgcgtt ctgtggagga catcatcgtg cagggcctgt gcaagagctt tgacggcgcg
     1141 gcggcgctgc gaaatttcag cgcccgcttc ccggcggggc gcatcacctg tgtgatgggg
     1201 cgctccggct gcggcaagac caccctgctg aacattttga tggggttcct ttccgccgac
     1261 gcgggcacgg tggccggggt gccggcgcgc cttgcggccg tctttcagga ggaccggctg
     1321 ctggagccct tttccgcggc gtccaatctg gcctttgtcc gccccaaagc ctcccccgcc
     1381 ctgatcggag agcaccttgc ggctgtcggg ctggcccagg cggcaagcca accggtgcgc
     1441 gagctgtccg gcggtatgcg ccgccgggtg gccattgccc gggcggtgct ggccggaggc
     1501 gagctgctga tcttggacga gccctttaag gggctggatg aggcgaccag gcgcgccgtg
     1561 gcctcttatc tgcggcgcta cacggccggg gccaccgtta tcctggtgac ccacgacgag
     1621 gacgaggtgg gccttatgga cggcgggctg atccgcatgg agccgccgtg cgcctgagcg
     1681 cttctgccgc ggctctcccc gccgatgcgc gggaaaaacg cgggccggaa gccttggccg
     1741 cgacggtaaa atacaagtga atataaaata agcgcgcccg gcggcacctc tgcgccgggc
     1801 gcctgtcaaa gcagaaaggc ggaggaacag gcaatgaaaa tcgagaccag ggcggaaata
     1861 ttggatattt taaagctgcc ccagcgggaa tacctcgcgg cggtcgcgcc cgaggcaaag
     1921 cgtgtgcaca gagaggaaaa cggcggccgc ctgacggcca ccgccatgct gggctacagc
     1981 aatgtgtgca aaaaccagtg cctctactgc gggatgcggg cggccaacag cgcggtgccc
     2041 cgctaccgca tcgagccgca ggacgtgctg gcctccctgg ccgccgccca tgaggcgggc
     2101 ttccggcggg tctttctcgt ctcgggagaa gacccgaagt atggctttga gcggctgctg
     2161 cagatcgtcg ccggggcaaa ggccctgggc atgggctggg tgagcctcgc ctgcggcgag
     2221 tacgagcgca gccagtatga agaactgcgc gccgccgggg cggacgagta tgtggtaaag
     2281 tttgagatgt cggaccggga gacctttgac cgactcaacc cctccaccag ctatgacaag
     2341 cggatgaacg cgatccgctg ggtgaaagag tccggcctgc tgctggcctc ggggaacatc
     2401 gtcgattatc ccggccagac gctggagcag ctggcggacg acatcctgct catgaaagag
     2461 ctggagatca gctgggcgcc cgtgatcccc tatctgcccg cccagaacac tccgctggcc
     2521 gccgagggcg gcccgggcag ccgtgagctg aacctgaaag agatctcgat tctgcgtttg
     2581 atgatgcccc aggtcaacat caccgcgcag cagccgggcg aaaatttggc cgacggcctt
     2641 gccagcgacg agggcaatct ggcggcgctg gcggcggggg cgaacttttt gttcgccgat
     2701 ctgctgccca ccgcgaaagc gcaggctttc cgggtggtgg acaaccgcat caccctgggg
     2761 ctggaccaca tccgggatat ggcgcgacgc agcggcatgg agttcacttt ttaacgcttt
     2821 tccgcgtgca gaggcacggc cggggcaagg gcccgcggca gcagcgaaat ccgcaaaaca
     2881 aaaaagggta tggccgtgat ttggccatgc cctttttcat gtggcgacaa aagcgcagca
     2941 acacaaaaaa agggggggac actctttgga gggatgcggc cagctctttc tgagtatact
     3001 tttttctctg cggagccggg ctacaaacgc cccgaatttt gttttgtcaa gctcgtacat
     3061 cggattcgcc tcctgcaaaa atgatagcgt tccgccgccg ggggcacaac cgattatcaa
     3121 gagagtcagc ccaaaaccgg ctttggcccc gaagagaatt caaaacagcg gcggatcaga
     3181 aaacgtttct gcaagctgct tgaaatatct ccccaaaggc ctccggcact tttttgtttt
     3241 ctaaacttaa tctaaaacga aaataattta atgtaaaaca ttaatttttt attgacaacg
     3301 tttaatttcc gtgttatgct tttgtcatcg ggaccgacga caatactgcg gaaagaatgt
     3361 gttttaccgt gaaaaagctc aatttgctcg ccttgatcgt ctctaaattt cttgagatcc
     3421 tgcactggct ggcagccgcg ctcatgctct gcctgctggc gctctgccta gccgcgccaa
     3481 actggctctc gggtcggctc gcccaagggg cgcagcggtt cggccgcgag ctgaccacct
     3541 atggttttac actgacagtg gccggcgccg acggcagtgt gaatcttgcc gccgtggctc
     3601 tatttaccgt gggggcgatt ttcatcctct cgctgatggc gatggtcttt cgcaacgtct
     3661 atctgatctt tcgcaccgcc aagggaaaga cctggtttgc caagggggat acgccgtttc
     3721 aaaaggatat cgtgcgcatg gtgcgcgaga tcggcatctt ttatctcgcg gtgcccatcg
     3781 tctcgctgct gatgagcgtc gtggctgtcc tggtgctggg ggcggacgcc gccgaaccca
     3841 gcgtcaactt tgagggcgtc gtcaccggca tcctgctgct ctgcctctcg caggtttttg
     3901 cctacggcgc ccagcttgag cgcgacgtgg acggcctgct ataaaagggg gataaaaaaa
     3961 tgggaaagat cgttctgcgg ctggaccgca tgatggtgga gcgtaagatg tcgttgaatg
     4021 agctggccca gcgggtgggc atctccaacg tcaatctctc caagatcaaa aacaatcggg
     4081 tgacggcgat ccgcttttcc accctggcgg ccatctgcga ggtgctggac tgtgaggccg
     4141 gggacatcct ggaatttcag cggggcgcac cggcagaggc cgacaaccct gaaaagggga
     4201 ttttgacggc gcgggaaaag ggcggcgctt aaacgcgaaa acgacggcgg gcggcagaca
     4261 ggggccgccc gccgtcgttt ttctgcgttt gcccgaccgg cgcgggggcc gcgcatgctg
     4321 cggaaaacac ctttgggtga aaccgccgtt ccgcacactt caaaaacagc ggccgcaaaa
     4381 agcgctctcc agcggcccgc gctgaaaaaa tgcaaagggt cccggtatcc gccgcaacgg
     4441 cagataccgg gactctttct tgcacctttt cagccctgct gcatcagata ctcctcgatg
     4501 aagtgggacg ccaccgcgcc gtcggacgcc gcggtcacga tctgccgcag cggctttgac
     4561 cgcaggtcgc ccaccgcaaa gacgccgggc agattggtgc gggtggtctc atccgcatcc
     4621 acatagccgt gttcctccag ccgcacctgc ccggcgaaaa gggcagtgtc gggcactctg
     4681 cccacggcca cgaacaggcc gtcgcagggc acctcccgct ccgcgccggt cttcacgtcc
     4741 gccacccgca ccccggtcac ctgcccgccg tgcaggatgc cggtgatgcg gctgttccag
     4801 aggaactcca ccccactctc tttcagagga gtgagataga cctgcgaggc gcgcagcgca
     4861 tcgcgccggt ggacgaggat cacttttttg cagagctttg ccaggtgcag cgcgtcggcg
     4921 gccgcgctgt tgccgccgcc cgcgaccacc acggtcttgc ccttgtagag catgccgtcg
     4981 caggtggcgc aataggccac cccgcggccg cgcagctccc gctcttccgg caggcccagc
     5041 tcccgcgggg aggcaccggt agagagcacc accgcccgcg ccccgatctc gccgtccgtg
     5101 gtcacgatgc gcttgatgtc gccagccagc tccaccgcgg tgacgtcggc aaacagggtc
     5161 tcggccccaa accgttccgc ccctcgctgc atgcgctccc ccagctcaaa gccgtcgatc
     5221 ccctcatcaa agccggggta gttatccacc tggccggtgg tcgccatctg accgccgggg
     5281 gccatctttt caatcacaac cactgaaaaa ccggcgcgcg cgcagtagag agccgccgta
     5341 tagccgcccg ggccgccgcc cagcaccgcc acgtccactt ttttgatacc catttttcca
     5401 ttgccccttt cacattacga tgccgccgtc cacatgcagg atcgtgccgg tggtgaaaga
     5461 cgcctcatcc gaggccaggt agacataggc cccggccagc tcctccggct gggccatccg
     5521 ccccagcggg gtcatcgcct tgaggccggc catcatccgc tcgtccaccg actgtgccac
     5581 catatcagtg cccaccacgc ccggggccac ggcattgacc cggatatgat agggccccag
     5641 ctcttttgcg caggatttgg tcagcccatt gaccgcgaac ttggaggagg aataggcggt
     5701 ctgcatcttg ccgccgtagg tgcccaccat ggaactggtg ttcacaatgc taccgccccc
     5761 ctgccgccgc atgatgggcg cgacctcccg cgtgcacttg aaagcgccgc ccaggttgac
     5821 ccgcagcacg gcgtcaaaat cctcgtcggt catatcgaaa atctgttttg ccgatgtgac
     5881 accggcgttg ttcaccagca cgtccacccg gccccaacgc cgcatcaccg cggccaccat
     5941 ggcgcgcacc gcctccgtat ccgctacgtc aacccccagc ggcaaaagct ccgccctcgg
     6001 cagctccgcg agcaactgct gtgccgcgcg ctcggcggag gagagcctgc tgccgcacaa
     6061 ggccacccgt gccccttctc ttgcaaacgc ccgtgccacc gcatagccga tgccccggga
     6121 cgcgccggtg atgaccgtca ctttcccctc cagcctcatc tcatcgcttc ctttcggttt
     6181 taattctttt gcactcaaaa cggctccgcc gccgggatgc gggcttctgc accgccgacc
     6241 agcggccgtg gggcctcaaa gggcgccgtt ttctctcagc cagttttcca ccgctgcctg
     6301 ggagggcggg tttaccgttc gcgccgcctc tttgccgcct gaaaacagga tcagcgtggg
     6361 gatggtgtcc acctgataac gccgggccag ctcaccgctt tcatcgatgt ccacccgcac
     6421 cacctcggcg gcctgccccg ctttctgggc cacccgttcc agcgctgggg agaggcgctt
     6481 gcagtacccg caccaatcgg ccataaaatc caccgccacc ggcttttccg accggaggac
     6541 ctcctgttca aaccctgcgc catctatctt tctgatcatc ataaaatccc actccttttt
     6601 tctgtttttc agttttatct tcgatcgatc tcccgctcac ccggcttctc cgctctcggt
     6661 ctcgtagggc caatctacaa tgccgccaaa gtcatatacg ttctggtagc ccaggtccac
     6721 cagtttttcg gcggcccgtt tgctgcgcac cccagtgcgg cagtggatca gcagcaccgc
     6781 ttctttttcc ggcagggctg cgggcggctg atcgccgatg gattccaccg gcaccagcac
     6841 cgcaccgggg atatggcttt ccttgtattc gtcctccctg cgcacatcca ccaccgtgac
     6901 gccgcccgcg tccatcatct ccttggcctc ctcggcactg atcttgtggt aggcctcctt
     6961 tgacccggca gtggagccgc cctgtgatgc ggagggttgc ccctcccccg cacaggctgt
     7021 aaacagcagc gcggccccga gggccagcac cggcaatccc gtccatctca tctctgtcct
     7081 ccctttcaat ctgtaatcat ttcgattaca tttactactg ttactatacc agttacagtg
     7141 cgtgttgtca actgtttttt gtctttcttc cggcctggcc cccaccggct gccgcaaagg
     7201 ggggcagaaa ggacaaccgg cggggggcca agcccactct ggaacaaaga ggggtcggaa
     7261 tcgccattgc ctttccatct gtgagcggga cctatttatt ctttcccgtt tctacaagaa
     7321 aagccgtctt gttcccttaa aaccgcagag ggaaatgtgc ctttatatca attttgagct
     7381 cccgtcgcgc gagggcgctt cccacagcaa acacagcaaa gccgggggaa gctgtacttc
     7441 ctccggcttt gcgggtgtcg agcctgtgaa aatcaaaaaa agaaaagcgc agccccccac
     7501 ggggcaaaag cgggtccgct caaaaaacga gggcggacgt cggcccaaaa tcaccgcagc
     7561 ccgtccctct tttctcgcct ccccgaagac aaaggaaaag ggcccggcag gggacaaata
     7621 cggctgaaac catctcgtcg taaatctgtg ccgccggaat ggagcggcgc tctcgggcgg
     7681 cccggtggcc cgggccacgc gccggaagcc cagggtctgg caaccgcagg gcgcggcgcc
     7741 gatcactggg tgaacagccc gtgcttgctg caataccagt acaactttcc cctgcgcacc
     7801 cggggcatgc gcagctctgc gccctgctcg gggtacagcc gcacaaggga cacgcggtcg
     7861 taccccaccg aagccacaaa ggcgatgtaa tgctcctttt ccatgtcgtg ggcaaaggtg
     7921 atgtagtctt catcctccac ctgctccacg acggggcgat gtccctcatc cgcgcaccgc
     7981 gcccgcagcg gctcaagacg gcgcccacag caggtcagct ccgccgcccc ggtggcggtc
     8041 aacaggttgc cgcaggacgg gcagatataa aattggatcc gtttcatatt tcctccctcg
     8101 gtatcgttgg cgtcaaggct gccggataag agccggtcca caccggtgcc cagcgcctct
     8161 gccagcgccc ccagggtgcc cacatccggg cagcccgcgc cgcgctccca ctttgagacc
     8221 gccttgtcac ccacgcccac ggcctcggcc agggcgcgct gggtcagccc cttttcaaga
     8281 cgcaggcggc ggatcagcgc cccggtcttt tgctggtcca ctccgatcac ctccgtccta
     8341 caggataccc cggaaagtga aaaaacacaa tcgacgcttc gtagaggcgt ttttttattg
     8401 ctgcgcaata aaaaaataac tgatttctat ggccgtggcc cggggacggg ccacatggcc
     8461 gtaaaaaata gcctgcggct attttttatc gctgcgcaac aaaaaactta ttataatggc
     8521 cacgtttctc gccgctgcgc ggcaaccgaa acggatgacc aaaacaatag ccgtacggct
     8581 attgtttatt ttgcgccgag cagggcatcc ggcggaattc cgcacagaga aacagcggca
     8641 gctgcgaggt gttgaccgcc gggtcatagg cctccggctc agtgagccgc acaaggctga
     8701 acccgctttc cgccgccaaa ttcaggtaaa acgaaaccgg gcgatgatag tgcagcgagc
     8761 tgccccaaaa ctcgttcagc tgtgcccgcg gccgcagata cccctgcacc agtttcgacg
     8821 tggcgatacc attttcatcc cgctgccagt cgccgataaa aaatgcgggg tggacgatgg
     8881 aaagccacaa aagccctgcc ggctttatca ctcgggaaaa ctcatgcatc gtaccctcga
     8941 tctccgccag atccatcagt acctgattgc aaaataccag gtcgaatatc ctgtcctcat
     9001 agggcagcgc cgcctgcagg t
//
LOCUS       DBMDYA010000022         8935 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2114_length_8935_cov_1.767376, whole genome shotgun sequence.
ACCESSION   DBMDYA010000022 DBMDYA010000000
VERSION     DBMDYA010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_8935_cov_1.767376"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="ACZPFK_01115"
     CDS             complement(<1..815)
                     /locus_tag="ACZPFK_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1404938.1"
                     /translation="MDPDGQSLILSAILFLLSLLSARALGAFSEIDGEEDDDAALLGL
                     TPLTGAAAVAAVLLLAGAVFCAAGWLRALPWAALAAALLGPVLYMLAFSLGAPRHPDE
                     APQSRAARVFSAVLGKPFLLLPRAIFRAAGLSAKSRVTEEDVLSLVDDVEEGDFIDES
                     QKEMISGVFELDDIAAGEIMTHRTEVEAVEDVGTAREVIPMALEKGFSRLPVYHKNLD
                     DIVGILYVKDLLRLVEDPAGGDVPIHQYLRPAMFVPESCRARELLLEFRAKRT"
     gene            1358..4921
                     /gene="nifJ"
                     /locus_tag="ACZPFK_01120"
     CDS             1358..4921
                     /gene="nifJ"
                     /locus_tag="ACZPFK_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1404939.1"
                     /translation="MARAKKSMDGNTAAAHVSYAFTEVASIYPITPSSPMADQVDQWS
                     AAHQKNIFGTEVKVMEMQSEAGAAGTVHGSLNAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPAVFHVSARTLATHALNIFGDHGDVYACRQTGFAMLADSNPQEVMDLGAVAHLSA
                     IKGRVPFINFFDGFRTSHEIQKIEVWDYADLKEMVDMDAVAAFRARSLNPEHPTMRGS
                     HENGDIFFQHREASNKYYDALPAIVEEYMNKVNAKIGTNYSLFNYYGAPDAERVIIAM
                     GSICDVAEEVIDYMMAAGEKVGLVKVRLYRPFVAEKLVEALPKTTKKIAVLDRTKEPG
                     ALGEPLYLDVVTALARCGVSIPVVGGRYGLGSKDTPPASVFAVYENLNQDKPKDNFTI
                     GIVDDVTGHSLEEKPAPDTTPAGTISCKFWGLGGDGTVGANKNSIKIIGDHTDKYIQA
                     YFQYDSKKTGGVTISHLRFGDKPIKSTYYINKADFVACHNPSYVVKGFKMVNDVKPGG
                     TFLINCQWTPEELEHHLPAEAKRYIAKNNIQLYTVNAIDKAIEIGMGKRTNTILQSAF
                     FSLAKIMPEEDAIKFMKEAAHKSYAKKGEAVVNMNYNAIDAGATAYVKIDVPASWADA
                     KDEDDGKALVGREALVKQVKEILDPVDMMNGDSLPVSAFVDHVDGTFEQGASAYEKRG
                     VAVTVPEWDPEKCIQCNQCSFVCPHATIRPFLLDEAEVKAAPASMKLVAAKGKGLEAY
                     KFAMTVSPLDCMGCGSCVNICPAPGKALKMVPQESQLPEQDAFDYAVAKVTKKETMFK
                     KDSVKGSQFEQPLLEFSGACAGCAETSYAKLITQLFGERMYVANATGCSSIWGGTGAT
                     SPYTVNRESGFGPTWANSLFEDNAEYGLGFQLGQKYIRETLVEKTRALIAVDYTDADL
                     KAAAQAWLDTLDEGDANLEAAKKYVAELKESVIEGCDCEACTLGREILSKKEYLSKKS
                     VWIFGGDGWAYDIGFGGLDHVLASGEDVNVMVFDTEVYSNTGGQASKASQIGQVAQFA
                     AAGKAIGKKDLAQIAMSYGYVYVAHIAMGADYNQTVKALKEAESYHGPSLIIGYAPCE
                     MHGIKGGMGNCQLEIKRAVASGYWHMYRFDPRAKAEGKPAFTIDSKAPTESYLDFIKS
                     ENRYARLQMAFPERAEALFDKAVSKSEDRYEYLKKLETLYK"
     gene            5448..6245
                     /gene="proC"
                     /locus_tag="ACZPFK_01125"
     CDS             5448..6245
                     /gene="proC"
                     /locus_tag="ACZPFK_01125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1404940.1"
                     /translation="MLDAKNVKIGFIGFGNMAQAMAKGLLATGAAQPDQICACAKNWE
                     KLCANTQPQGMRPCRDAAELAACADLVVVAVKPYLVAEVLAPIKEQLREKIVVSVAAG
                     MPFEKYEQILAPGTHHLSTVPNTPISVGEGIIACESRHSLTKEEWALFEALFGKAALV
                     QPVDTAQLSVAGTLAGCGPAFAAMFVEALADGAVQQGLPRALAYRLASQMMVGTGKLQ
                     LATGAHPGAMKDAVCSPGGTTIVGVAELERHGFRSALIDALDAIEHK"
     gene            complement(6350..7762)
                     /locus_tag="ACZPFK_01130"
     CDS             complement(6350..7762)
                     /locus_tag="ACZPFK_01130"
                     /inference="COORDINATES: protein motif:HMM:NF026253.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR1404941.1"
                     /translation="MRRILSRFLCPLLCLCLLAGLTGCGELPAPGGKPSSASSGAAGS
                     SQATGVSSEKGMLQPLTQDAFGYGEFFHNGYAFAVRDNDCGYLDLQGNFTPYYKIPSS
                     GLAGSVDLSLLFEEYTSLLDQFDHNQMFVSEEGLFPWYDEDTGLWGYGDIHTGKWVIE
                     PAYLDAFPFSEQRAVVCPGRDGAAQVIDTDGKVLFEIGAPLELCYDTGSLHALGPVPG
                     GSPDHAYLIGRDGDILAELPCFRTQDGGNMHRIPDMPGGRIDRADPDHILVVGAVVQP
                     NGKLKFSNFYYLDRDGNEVVRPPQGATWAYPWEESAKATFYEMGENQFGIFNENGKIT
                     EPIYSDFYFLYGDYTFVQENDESWHLIDAQGRRVGEEALEECTHFGENGLAAVCKNGL
                     WGYLDTKGQFVIEPTFVRATIFDGGTAVAGLEGETRSFFINEEGKAISDKLELIAAYW
                     DKDTFLYQDGQEAYHYLGRA"
     gene            8339..>8935
                     /locus_tag="ACZPFK_01135"
     CDS             8339..>8935
                     /locus_tag="ACZPFK_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1404942.1"
                     /translation="MNILLVEDETAIAAPLASLLRGEGWRVDICGRVDEALARLERNR
                     FDAALVDLGLPDGDGAEVCRAVRCAGDAAVIILTARGDEASVVRGLEQGADDYVTKPF
                     RGRELISRLRAVQRRRSPSPARLGSLLIDRQAAQVRREGRVLALTAQEYRLLLAFAAH
                     PGQLLERGSLLQALWDQGGSFVNDNTLTVTVKRLREKID"
BASE COUNT         1848 a         2797 c         2780 g         1510 t
ORIGIN      
        1 tgggtgcgct tggcgcggaa ctccagcagc agctcccggg cgcggcagct ctcgggcaca
       61 aacatggccg ggcgcaggta ttggtggatg ggcacgtcgc cgcccgccgg gtcctccacc
      121 agccgcagca ggtctttcac gtacaaaata cccacgatgt cgtccaggtt tttgtggtag
      181 accggcagcc gggaaaagcc cttttccagc gccatgggga tcacctcgcg ggccgtcccc
      241 acatcctcca ccgcctccac ctcggtgcga tgggtcatga tctcgcccgc ggcgatatcg
      301 tccagctcga acacgcccga gatcatctct ttctggctct cgtcgatgaa gtccccctcc
      361 tccacgtcgt ccaccagcga gagcacgtcc tcctcggtca ccctgctctt ggccgacagg
      421 cccgcggcgc ggaagatcgc ccgcggcagc agcaaaaacg gcttgcccag cacggcggaa
      481 aagacgcggg cggcgcggct ttggggcgcc tcgtcggggt ggcggggcgc gcccagcgaa
      541 aaggccagca tgtagagcac cggccccagc agcgcggcgg ccagggccgc ccagggcagg
      601 gcgcgcagcc agccggcggc gcagaagacc gcccccgcca gcagcagcac cgccgccacg
      661 gcggcggcgc cggtgagcgg ggtcagcccc agcagggcgg cgtcatcgtc ctcttcccca
      721 tcgatctcgg aaaaggcgcc cagcgcccgg gcggacagca gcgacagcag aaacagaatc
      781 gcggagagga tcaaactttg accatcaggg tccatgcgga caaaactccc ttttctcggc
      841 ggcgggccgc cgtttttatc tacagacgat tttatagtat ttgaggggcg gtgtcaaacg
      901 ccgcccgcac cggcggccgt tttttgatgt tctgcggcgc tttgggcgcg gggaggcggg
      961 gaccgcccgg caaaaagggc gggggtaccg cctgggtacg gcggcacgcg ggcctcggcc
     1021 ttggagagag aaagaccgca ggcgcgcccg gctttttcat cggaaagcgg attttgcggc
     1081 gcggcggagg tcccaaagcc gttgcgggca gggctggcgg cggggatttt gagccccggg
     1141 cccgcagaaa aaacgggctt tgaggggaaa gagcgccccg tctgcgagga tttggaaaaa
     1201 ttgccataaa tttaaggata ataacactat tttaatgaca aattcgtgag aaaagccggt
     1261 tttgttattt acttatcaag ccggatttga taaaataatg aatgcgagag cgcaatccat
     1321 cgttacgcta tcaatttttt agggaggaac taaaaaaatg gcaagagcta aaaagagtat
     1381 ggatggcaac accgccgccg cgcacgtaag ctacgcgttc acggaagttg ccagcatcta
     1441 tcccatcact ccgtccagcc ccatggccga ccaggtggac cagtggtccg ccgcgcacca
     1501 gaagaacatt tttggtactg aagtcaaggt catggagatg cagtcggagg cgggcgccgc
     1561 cggcaccgtg cacggcagcc tgaacgcggg tgcgctgacc accacctaca ccgcgtccca
     1621 gggcctgctg ctgatgatcc ccaacatgta caagatcgcg ggcgagctgc tgccggccgt
     1681 gttccatgtc tcggcccgta ccctggccac ccatgcactg aacatcttcg gcgatcacgg
     1741 cgacgtgtac gcctgccgtc agaccggctt tgcgatgctg gccgactcga acccgcagga
     1801 ggtcatggac ctgggcgccg tggcccatct gtccgccatc aagggccgtg tgccgttcat
     1861 caacttcttc gacggcttcc gcaccagcca cgagatccag aagatcgagg tctgggacta
     1921 tgccgacctg aaagagatgg tggacatgga cgccgtggcc gcgttccgcg cccgctcgct
     1981 gaacccggag caccccacca tgcgcggctc gcacgagaac ggcgacatct tcttccagca
     2041 ccgcgaggcg tccaacaagt actatgacgc cctgcccgcc attgtggaag agtacatgaa
     2101 caaggtcaac gccaaaatcg gcaccaacta cagcctgttc aactactacg gcgcccccga
     2161 cgccgagcgc gtcatcatcg ccatgggctc catctgcgat gtggccgagg aggtcatcga
     2221 ctacatgatg gccgcaggcg agaaggtcgg cctggtcaag gtgcgcctgt accgcccgtt
     2281 cgttgccgaa aagttggtcg aggccctgcc caagaccacc aagaagatcg ccgtcctcga
     2341 ccgcaccaaa gagccgggcg ccctgggcga gcccctgtat ctggacgtcg tcaccgctct
     2401 tgcccgctgc ggcgtctcga tccccgtcgt cggcggccgc tacggcctgg gctccaagga
     2461 caccccgccc gccagcgtct tcgccgtgta tgagaacctg aaccaggaca agccgaagga
     2521 caacttcacc atcggcatcg tggacgacgt caccggccac tccctggagg agaagcccgc
     2581 ccccgacacc accccggccg gcaccatcag ctgcaagttc tggggcctgg gcggcgacgg
     2641 caccgtcggc gcgaacaaga actccatcaa gatcatcggc gaccacaccg acaaatacat
     2701 ccaggcgtac ttccagtacg actccaagaa gaccggcggc gtcaccatca gccacctgcg
     2761 cttcggcgac aagcccatca aatccaccta ctacatcaac aaggccgact tcgttgcctg
     2821 ccacaacccg tcctatgtgg tcaagggctt caagatggtc aacgacgtca agccgggcgg
     2881 cactttcctg atcaactgcc agtggacccc tgaggagctg gagcatcacc tgcccgccga
     2941 ggccaagcgc tatatcgcca agaacaacat ccagctgtat accgtcaacg ccatcgacaa
     3001 ggccatcgag atcggtatgg gcaagcgcac caacaccatc ctgcagtccg cgttcttctc
     3061 cctggccaag atcatgccgg aagaggacgc catcaagttc atgaaagagg ccgcgcacaa
     3121 gtcctacgcc aaaaagggcg aggctgtggt caacatgaac tacaacgcca ttgacgcggg
     3181 cgccaccgcc tacgtcaaga tcgacgtgcc cgccagctgg gccgacgcca aggacgagga
     3241 cgacggcaag gcgctggtcg gccgcgaggc gctggtcaag caggtcaagg agatcctcga
     3301 cccggtcgac atgatgaacg gcgacagcct gcccgtctcc gctttcgtcg atcacgtgga
     3361 cggcactttc gagcagggcg cttccgctta cgagaagcgc ggcgtcgcgg tcaccgtgcc
     3421 cgagtgggat cccgagaagt gcattcagtg caaccagtgc tcgttcgtct gcccgcacgc
     3481 caccatccgc ccgttcctgc tggacgaggc cgaggtgaag gccgcccccg cttccatgaa
     3541 gctggtcgcc gccaagggca agggcctgga ggcctacaag ttcgccatga ccgtttcccc
     3601 gctggactgc atgggctgcg gctcctgcgt caatatctgc ccggcccccg gcaaggcgct
     3661 gaagatggtg cctcaggaga gccagctgcc cgagcaggac gcgttcgact acgcggtcgc
     3721 caaggtcacc aagaaagaga ccatgttcaa gaaagactcg gtcaagggca gccagttcga
     3781 gcagccgctg cttgagttct cgggcgcctg cgcgggctgc gcggagacca gctatgccaa
     3841 gctgatcacc cagctgttcg gcgagcgcat gtacgtggcc aacgccaccg gctgctcctc
     3901 catctggggc ggcaccggcg ccaccagccc ctacaccgtc aaccgcgaga gcggctttgg
     3961 cccgacctgg gcgaacagcc tgttcgagga taacgccgag tacggcctgg gcttccagct
     4021 cggccagaag tatatccgcg agaccctggt ggaaaagacc cgcgccctga tcgccgtcga
     4081 ctacaccgac gccgacctga aggccgccgc ccaggcctgg ctggacaccc tcgatgaggg
     4141 cgacgccaac ctggaggccg ccaaaaaata tgtcgctgag ctgaaagagt ccgtcatcga
     4201 gggctgcgac tgcgaggcct gcaccctggg ccgcgagatc ctctccaaga aagagtatct
     4261 gtccaagaag tccgtctgga tcttcggcgg cgacggctgg gcctacgaca tcggcttcgg
     4321 cggcctggac cacgtgctgg cttccggcga ggacgtcaac gtgatggtct tcgacaccga
     4381 ggtgtactcc aacaccggcg gtcaggcctc caaggccagc cagatcggcc aggtggcgca
     4441 gttcgccgcg gccggcaagg ccatcggcaa aaaggatctg gcgcagatcg ccatgtccta
     4501 cggttacgtc tatgtggcgc acatcgccat gggcgccgac tacaaccaga ccgtcaaggc
     4561 gctgaaagag gctgagagct atcacggccc ctcgctgatc atcggctacg ccccctgcga
     4621 gatgcacggc atcaagggcg gcatgggcaa ctgccagctg gagatcaagc gcgccgtcgc
     4681 ctccggctac tggcacatgt atcgcttcga tccgcgcgcg aaagccgagg gcaagcccgc
     4741 tttcaccatc gactccaagg cgccgaccga gagctatctg gacttcatca agtccgagaa
     4801 ccgttacgcc cgtctgcaga tggctttccc cgagcgcgcc gaggcgctgt tcgacaaggc
     4861 ggtcagcaag tccgaggatc gttacgagta tctcaaaaag ctggagaccc tttacaaata
     4921 agagccttgc cagcctgaag tacgctccct aaaaattggg cgcccccacc gggatttccg
     4981 gtgagggcgc cctttttctg cttcgtctgc gggaggctgt gagctttgcg gcggtagccg
     5041 gggcgataca aaagcggccc gagcgcccgg ccccgcgcag acggctgcag gagcagagcg
     5101 cagagaagat tttcaccagg tcgttttctt attgctgcgc aacaaaaaac aactgattct
     5161 aatggccgcg tttctcgccg ctatgcggca accgaaacgg atggccgaaa aaatagcctg
     5221 cggctatttt ttatttgctg tcccagcttt tgcaggggcc gctgttttcc cacggccctt
     5281 ttggggcgcg caaagcgaaa aagcgaagtg cgcggttcgc tatttttggg gtaaagcgat
     5341 aaaaatactt gccaagcggc caggatgcgg taaactaaga gaggatgcac ggcacgaaac
     5401 agtcggctgc cggcggtccg gccgccgcag aaaaaggaga gagcgaaatg ctggatgcaa
     5461 aaaatgtgaa aatcggcttt atcggctttg gcaatatggc ccaggccatg gcaaagggcc
     5521 tgctggcgac cggcgcggcg cagccggacc aaatctgcgc ctgcgcaaaa aattgggaga
     5581 agctctgtgc caacacccag ccccagggca tgcggccctg ccgggacgcg gcggagctgg
     5641 ccgcgtgcgc ggacctggtg gtggtggcgg tcaagcccta cctcgtggcg gaggtgctgg
     5701 cccccatcaa agagcaattg agagaaaaga tcgtggtctc ggtggcggcc gggatgccgt
     5761 ttgaaaaata cgagcagatc ctggcccccg gcacccatca tctgagcacg gtgcccaaca
     5821 cccccatctc ggtgggtgag ggcatcatcg cctgcgagag caggcattcg ctgacaaaag
     5881 aggaatgggc gctgttcgag gcgctgttcg gcaaggcggc gctggtgcag ccggtggaca
     5941 ccgcccagct gagtgtcgcg ggcacgctgg cgggctgcgg cccggccttt gcggccatgt
     6001 ttgtggaggc gctggccgac ggtgcggtgc agcagggcct gccccgggcg ctggcgtacc
     6061 ggctggccag ccagatgatg gtgggcaccg gcaaactgca gctggccacc ggcgcgcacc
     6121 ccggggcgat gaaagacgct gtctgctcgc ccggcggcac caccattgtg ggggtggccg
     6181 agctggagcg ccacggcttc cgcagcgcgc tcatcgacgc gctggacgcc atcgagcaca
     6241 aataaaaaac aaaaaggaca gccgcgttgg cccgagggga aaaagcccca aagccaacgc
     6301 ggctgtctta tattttttaa tccggcgtca ggcggcataa aggccgttgt caggcgcgcc
     6361 cgagatagtg ataggcctcc tgcccgtcct gatagagaaa ggtatctttg tcccaatagg
     6421 ccgctatgag ttccaactta tccgaaattg cctttccctc ttcattgata aaaaaggaac
     6481 gcgtttctcc ctccagaccg gcaacggcag ttccgccgtc aaaaatggtg gcccggacga
     6541 acgtgggctc gatgacgaac tgtcctttgg tatccaagta gccccacagc ccgtttttgc
     6601 aaaccgccgc cagaccattt tctccaaagt gagtgcattc ttccagtgcc tcttctccca
     6661 cccgccggcc ctgagcgtcg atcagatgcc agctttcgtc gttttcctgg acgaaagtat
     6721 agtctccgta tagaaagtag aaatcgctgt agattggttc ggtgatcttt ccgttttcgt
     6781 tgaaaattcc aaactgattt tctcccatct catagaaagt agccttggca ctttcctccc
     6841 aggggtaagc ccaggtggcc ccctgcggcg gccggacgac ctcgttgccg tcccggtcca
     6901 gataataaaa attgctgaac ttcagttttc cattaggttg gaccaccgcc cccaccacaa
     6961 gaatatggtc cgggtctgcg cggtcgatcc tgccgcccgg catgtcgggg atgcggtgca
     7021 tgttgccgcc gtcctgggtg cggaagcagg gcagctcggc caggatgtcg ccgtcgcggc
     7081 cgatcagata ggcgtggtcg gggctgcccc cggggacggg gccgagggcg tgcaggctgc
     7141 cggtatcgta acagagctcc agcggggccc cgatctcaaa cagcaccttc ccatcggtgt
     7201 cgatgacctg agcggcgccg tcccggccgg ggcagacgac cgcccgctgc tcgctgaaag
     7261 ggaacgcgtc cagataagcg ggctcgatga cccacttgcc ggtgtggatg tccccgtatc
     7321 cccacaggcc ggtgtcctcg tcgtaccagg ggaaaagccc ctcctccgac acgaacatct
     7381 gattgtggtc gaattgatcc aacagggagg tgtactcctc aaagagaaga ctcagatcca
     7441 cactgccggc aaggccgctc gacggtatct tgtaataggg ggtaaagttg ccctgcaggt
     7501 ccagataacc gcagtcgttg tcccgcaccg caaaggcata gccgttgtga aaaaattccc
     7561 catagccgaa cgcatcctgg gtcaggggct ggagcatccc tttttcagac gagacgccgg
     7621 tcgcctgaga ggagccggca gcgcctgagg aggcggaaga cggcttgccg ccgggggcgg
     7681 gcagctcacc gcagccggtc agcccggcca gcaggcagag gcagagcagc gggcagagaa
     7741 agcgggataa gattcgtctc ataaagcaac ctcctgaaag taaaaaatgg ccgcaggctg
     7801 ttttttcggc catccgtttc gattgtcgcg cagcgacgag aaacggggcc attaaaataa
     7861 gccgtttttt attgcggagc gctaaaaaca gccgcaggct gtttttcggc catccggcca
     7921 gtcccctggc cgtggccatt aggataaatt attttttagt gcggagcact aaaaaacagc
     7981 cgcaggctgt ttttcagccc tatggcccgt ccccgggcca cggccctaga aataaaatgt
     8041 ttttatgtaa aaagcaataa aaaacaggcg cgaaacaggg caaaaaataa attgttgccc
     8101 cctgatttga ctccaggata agccaaaacg cagagaattg caagagagaa tcgaatcctc
     8161 ccgcttttgc ccccgccgct cgtcctgccc gtgacggcag gcggagcagt ggagaaaaag
     8221 atgacggaaa cgtcacgcac ggcggggctt gttctgatag aatagaacgt gacagaaccc
     8281 gattggctgc gggccgccgc gcgcgcctcg gcgcaaacgc cggtacagga ggacaaaaat
     8341 gaacatctta ttggtggagg acgagacggc cattgccgcg ccgctggcca gcctgctgcg
     8401 gggcgagggg tggcgggtgg acatctgcgg ccgggtggac gaagcgctgg cgcggctgga
     8461 gagaaaccgt tttgacgccg cgctggtgga cctgggcctg ccggacggcg acggggccga
     8521 ggtctgccgc gccgtgaggt gcgccgggga cgcggcggtg atcattttga ccgcgcgggg
     8581 cgacgaggcc agcgtggtgc gcgggctgga gcagggggcg gacgactatg tcaccaagcc
     8641 gttccgcggc cgcgagctca tcagcaggct gcgggcggtg cagcgccgcc gcagcccgtc
     8701 gccggcccgg ctgggcagcc tgctgatcga ccggcaggcc gcccaggtgc gccgggaggg
     8761 gcgggtgctg gcgctgacgg cgcaggaata ccggctgctg ctggcgtttg ccgcgcaccc
     8821 gggccagctg ctggagcggg gcagcctgtt gcaggccctg tgggaccagg ggggcagctt
     8881 tgtcaacgac aatacgctca ccgtcaccgt caagcggctg cgggagaaga tcgac
//
LOCUS       DBMDYA010000023         8769 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2155_length_8769_cov_1.444393, whole genome shotgun sequence.
ACCESSION   DBMDYA010000023 DBMDYA010000000
VERSION     DBMDYA010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2155_length_8769_cov_1.444393"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3062)
                     /locus_tag="ACZPFK_01140"
     CDS             complement(<1..3062)
                     /locus_tag="ACZPFK_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404943.1"
                     /translation="MRTICPKNMAGRQGVFRRALAALMSFCLLLTCLNASFLAARAAE
                     SSSVALPVGAEVTAVLQNGKLTLTGTGDTYDFSAETAPFAAYAGEVRTLVVEDGVTGL
                     GAYLFYGMRELGGELTLPASVVRFGDCVFSGASLQEAPHFTVIRNLFESAALAPTEPE
                     ALSNEPTPAAEADASTQAEDAAVSLSEEAPPAGAQPQEPLPGDDTEGQTAHSEAQSAS
                     TPEASSSSQPEATAPPVAGSAEPGLPEAGGNQEDTAQPATPPAQTEQNADGLLPSGAA
                     QTEPEESAQATPVTVYQQQIARPETLFSAGQSGKFVCSPQNESFRQAALAAGYEAAEG
                     VAQVELDGRITLELPVADGRLTLPEVPEQLTGPKSDNIFFTQVFAGWTQSPDDPLAPV
                     LAAGTLFAAKDGEWVSLYSAWSEKERYQFRIKAARWDGTAEYTLTDDGTGEPVTPPAG
                     YTFLYQWQSAPQDGGSGAEWTDVEGETASVYRRQIDPADQKLQFRCKVTAVGLARSAG
                     ERPTLYSDPVNGVTTEKTVYVDQTAGSDVNGDGTESAPYASIEKAAESLDKSGTVDTN
                     RIVIAGTYHLNFQGAQEKIDMAKICGVPVTIAGRPGDSAAVFQAPMYQATATSQTPAA
                     MTLASDLRFENIAVNNIDHIYGSGYDITLGTGVTNNKDFYLYGSGQNAITEEIGAITV
                     YSGRFARLVGYVRSTATPLDANGKRAVITVGGTANVGTIIAGCASGAIKNGDVAITVE
                     GGTIGTLVGGNQGFNKGAAPYTGKTVISISGGNVTNVFGAGTGRNYSVPTYCGEMEIE
                     VTGGKVSNIYGAGSAAYVVSDGTTRSKVNIFVRGAAEVGNIFAAGRGGETMDGPEEGT
                     DPSDYGSLTGDAEIKVSGNAVVTGDVYASGQGYKDSSKYDTTQNAYLNGNVTITVEGG
                     ATVQGSVYGGGQGFADAGYENCARVKADSTVEVRVADGTVKGSVYGGGQTAKMEGSTS
                     VVISGGTVEGNVYGGGEAGVVEGAVSVRVSGGTVESSVYGGALGEVGTT"
     gene            complement(3115..3846)
                     /locus_tag="ACZPFK_01145"
     CDS             complement(3115..3846)
                     /locus_tag="ACZPFK_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404944.1"
                     /translation="MPKKRARRGRKQKIGAIVLLLMGMGALVLAGGVLARYVLFWDSE
                     NAVVQPDAFYFTSDLLREGAAPSYQIDPQIKGIEIKLFNAEDEKRVTASKISYKVEVE
                     GGTADRAEGVIQGGAAGNAGAVVKITPADGAAEVVVTVVSESPYVKTLSGKFVREAGD
                     AFTVEDKSGNRAAVLTITATEPAQKVTITLPESVIPDATDSRVAKEGNNYVFQPVDAG
                     VYSLVLLKNNPDTLLTADSVTVSFS"
     gene            complement(3882..4433)
                     /locus_tag="ACZPFK_01150"
     CDS             complement(3882..4433)
                     /locus_tag="ACZPFK_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404945.1"
                     /translation="MKRQLTVGRVAVYLLCLSAAFALVVGVSYSRFTGTVRGSGGAAV
                     AAVAMEGTSNVDLTTALAGLAPGTSRTISFTVANAKNGVVSEVAQSYVVEVETTGNLP
                     LTFSLVGTTPAANEGRLADLNDGAAAVWENGLLPAGKQVNHLYTLTVAWPKDKSDAAY
                     AREVDAVSLHIRAVQALPEAAGK"
     gene            complement(4495..5976)
                     /locus_tag="ACZPFK_01155"
     CDS             complement(4495..5976)
                     /locus_tag="ACZPFK_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR1404946.1"
                     /translation="MLILLALLALFSSVIAGFLIGRNSGPALGQIIDTIVIGPDQSRT
                     VHLAGQVRYADGTPYTGGTVELRSEPRRTDTDAQGRFFYDSVELGDHTLSVVDAQGAV
                     LAACEIAVGRGGSQPLEVAETAEGRYTVELSTEVRFIELAVELRGEKAALVLLPGAAV
                     ALQDDGTVFVDKSSLTAAAGEIVLPSGTVILKDSTVVAPGYLILPDNMVTPIPTGGTT
                     TGGGEKILPDGRVELADGTVISAEGIRTPDGAVLSPEKPYQIAAGGQTPRPSPNGQAG
                     TQGRPGQGGSAGSQPSRSGGDGGTSSSQGHGGDGSSSAPAPTPGPTSSLEPTPTPSLP
                     PDSSEPEDDDKGVLEAQQSAVGGWSSWESESGVDLFYNRTGAAANGNIQPGSRGSYSF
                     RLVNSRRDALSYEISLSEASFHLPMRFRLVDADGSAGEWQTLKKGQTLRLGGGTVGAE
                     PLVLGVEWEWLYEGGDDAADTAAGAGQSEEDRAYIVSLKVHAG"
     gene            complement(6225..6830)
                     /locus_tag="ACZPFK_01160"
     CDS             complement(6225..6830)
                     /locus_tag="ACZPFK_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404947.1"
                     /translation="MKKKAIGRLGVLALALTLISTCLMGGTLAKYTADVTGSATATVA
                     KFSFDLNGVTEKTEIKTIDLSTLFSKTYNSNNVSASSAVVAPGTSGKVKIELTNKGEV
                     AIKPDFTITETNIDKIPLQYAITATEAAPAEEQNWKAATDLAPTETAVVVGANAQTFY
                     LHWRWNPSSTDAADTALGSASALAEAKLEIKCVVTQVIPTA"
     assembly_gap    7144..7153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7154..7738)
                     /locus_tag="ACZPFK_01165"
     CDS             complement(<7154..7738)
                     /locus_tag="ACZPFK_01165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1404948.1"
                     /translation="MKEKRKRSGRAAGVVSVLLCVLFFPVILVNVIIIANTYLHPDEI
                     PGAFGVKPVAVLSGSMEDTFMTGDLIFLHKTDPASLQEGDVICYLVSGQAVTHRIVRV
                     ERDGGGATRYITKGDANNAEDAEPVLPQQVQGIWKGGRIGGAGNAVLFLSSTTGMLLF
                     IVCPILLLIVWDIVTRRKTDKNEKARADALEAELK"
     gene            complement(7900..8754)
                     /locus_tag="ACZPFK_01170"
     CDS             complement(7900..8754)
                     /locus_tag="ACZPFK_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1404949.1"
                     /translation="MKNSVWNREALNRFQSCLICEERSPRTIEKYMRDAEAFCRFLPR
                     DKQLCKEQVIAYKARLAQSYKASSANSMLVAVNRFLAFLGRQDCRVKLFKIQRSNFRQ
                     AEKELSKEEYRRLLKVARSKKNERLYLLMQTLCGAGLRVSEHRFVTVEALRKGSVRVR
                     NKGKERLVFLPPELVKQLQAYCAHCRITAGPVFVTRSGRALDRSNIWAEMKRLCREAR
                     VERKKVFPHNLRHLFALTYYRLQKDIVRLADVLGHSSVETTRIYTVTSGREEKRLIAR
                     LGLIEGSA"
BASE COUNT         1479 a         2960 c         2442 g         1878 t
ORIGIN      
        1 gtggtgccca cctcgcccag cgcgccgccg taaacagagc tctccacggt gccgccgctg
       61 accctcaccg acacggcccc ttccacgacg ccggcctccc cgccgccgta gacattgccc
      121 tccacggtgc cgccgctgat aacgaccgac gtagacccct ccattttggc ggtttgtccg
      181 ccgccgtaaa cgctgccctt gacggtgccg tccgcgaccc gcacctccac ggtggaatcc
      241 gcttttaccc gggcgcaatt ctcatacccg gcatcggcaa agccctggcc gccgccgtaa
      301 acgctgccct gcaccgtggc gccgccctcc acggtgatgg tgacgttacc gttcaaatag
      361 gcgttttggg ttgtgtcata tttgctgctg tccttatatc cctgcccgct ggcatagacg
      421 tcgccggtga ccaccgcgtt cccgctcacc ttgatctccg cgtcccccgt caggctgccg
      481 taatcactcg gatcggtgcc ctcctcgggg ccgtccatgg tttcaccgcc tcttcccgcg
      541 gcaaagatgt tgcccacctc ggcggcgcct ctgacaaaaa tattgacctt ggaacgcgtc
      601 gtgccgtcgc tgacgacata ggccgccgat cccgcgccgt aaatattgct cactttgccg
      661 ccggtgacct cgatctccat ctctccgcag taggtgggca cgctgtaatt tcgacccgtg
      721 ccggcgccga aaacgttggt cacatttccg cctgagatgg aaataacggt tttgcccgta
      781 tagggggccg cgcccttgtt aaagccctga ttccccccca ccaatgtccc tatggtgccg
      841 ccctcgaccg tgatggcgac atctccgttt ttgatcgcac cgctggcgca gcccgcaatg
      901 atcgtgccga cgtttgcggt gccgcccact gtgatgacgg cccttttccc gtttgcatcc
      961 aacggggtcg ccgtgctgcg gacatagccc acaagcctgg caaatcttcc gctataaacg
     1021 gtgatcgcgc cgatctcctc cgtaatggcg ttctgtcccg agccatacag ataaaaatcc
     1081 ttgttgtttg tcactcccgt ccccagggtg atgtcatatc cgctgccata gatatggtcg
     1141 atattgttga cggcgatatt ttcaaagcga agatcgctcg ccaacgtcat ggccgccggc
     1201 gtctgcgatg tcgccgtcgc ctggtacatc ggcgcttgaa agacagccgc actgtcccca
     1261 ggccgccccg cgatcgtgac cggaaccccg catatcttcg ccatatcaat tttttcctgg
     1321 gcaccctgaa agttcagatg ataggtgccg gcaataacaa tcctgttggt gtctacagtc
     1381 ccacttttgt ccaggctttc cgctgctttt tcgatagaag catagggggc tgattctgtc
     1441 ccgtccccgt tgacgtcgct tccggcggtc tgatccacat aaacggtttt ttcggtggtc
     1501 acgccgttta ccgggtcgga atacagcgtg ggccgctcac ctgccgaccg ggccagcccc
     1561 accgccgtca ccttgcagcg gaattggagc ttctggtctg cggggtctat ctgccgccgg
     1621 tagaccgatg cggtttcccc ctccacatcc gtccattccg cgcctgaccc gccgtcctgc
     1681 ggcgcgctct gccactgata caaaaaagta tagcccgccg gcggcgtcac gggctcgccc
     1741 gtgccgtcat ccgtcaaagt atattccgcc gtgccgtccc accgcgcggc ttttatccgg
     1801 aattgataac gttctttttc gctccatgcg gaatagagtg aaacccattc cccgtctttt
     1861 gccgcgaaaa gcgtgcccgc cgccaatacc ggggcaagag ggtcgtccgg gctctgggtc
     1921 catccggcga atacttgagt gaaaaaaatg ttgtcgcttt tcgggccggt gagctgctct
     1981 ggaacttcgg gcagcgtcag ccgcccgtcc gccacaggca gctccagcgt tatccggccg
     2041 tccagctcca cctgggcgac gccctctgcg gcctcatacc ccgccgccag cgcggcttga
     2101 cggaaagact cgttctgtgg ggagcagaca aatttaccgc tctggcccgc tgagaacagc
     2161 gtttccggcc gcgcgatctg ctgctggtaa accgtgaccg gcgtggcttg cgccgattct
     2221 tcgggctccg tctgggcggc gccggacggc aataagccgt cggcgttctg ttccgtttgg
     2281 gccgggggcg tggctggctg ggccgtgtcc tcttgattac cgcccgcttc cggcaggccg
     2341 ggctcggcgc tccccgccac cggcggcgcg gtcgcctcag gctgcgaaga ggaagaggct
     2401 tccggtgttg aggcggactg cgcctctgaa tgcgcagtct gcccctctgt gtcgtcgccc
     2461 ggcagcggct cctgcggctg cgcgcccgcg ggcggcgctt cctcggaaag gctcactgcg
     2521 gcgtcctccg cctgtgtcga tgcgtccgct tccgcggcgg gcgttggctc atttgaaagg
     2581 gcttctggtt ccgttggggc aagtgccgcc gactcaaaca gattgcggat caccgtaaaa
     2641 tggggcgctt cctgcaggga cgcgccggaa aaaacgcagt cgccaaaacg gacgacgctt
     2701 gcgggcagcg tcagttcgcc gcccagctcc ctcatcccat aaaaaagata tgctcccagg
     2761 ccggtgacgc cgtcctcgac caccagcgtg cgcacctcgc ccgcataggc ggcgaacggt
     2821 gcggtctcag cgctgaaatc ataagtatcg ccggtgccgg tgagcgtcag cttcccgttt
     2881 tgcaagacag cggtgacctc cgcccccacc ggcagcgcta cagagctgct ctccgcagcg
     2941 cgggcggcca ggaagctggc gttcaaacag gtcaacaaaa ggcagaacga catcagtgcc
     3001 gccagcgcgc gccgaaacac cccctgacgg cctgccatat tttttgggca tatcgtcctc
     3061 attgaagttc ctccatttga tcgccggcga cctcgttttt caacaaaatc acccctaaga
     3121 aaaagatacc gtcacgctgt ccgccgtcag cagcgtatcg ggattatttt ttaacagcac
     3181 cagggaatag acgcctgcat ccacgggctg aaacacgtaa ttgtttccct cttttgcgac
     3241 gcggctgtcg gtggcatccg ggattacgga ctcgggtaaa gtgatggtga ctttctgcgc
     3301 aggctcagtc gccgtgatgg tgagaaccgc cgcccgattg ccgcttttgt cttccaccgt
     3361 aaaagcgtcg ccggcctccc tgacaaattt tccactcagc gttttgacgt agggtgattc
     3421 tgagacgacc gtgaccacga cctcggccgc tccgtcggcc ggggttatct tgacaaccgc
     3481 gcccgcattg cccgcagcgc cgccttggat cacaccctcc gccctatccg ccgtgccgcc
     3541 ctccacttcc accttgtagc tgatcttcga ggccgtgacc cttttttcat cctcagcgtt
     3601 aaacaacttg atctcgatgc ccttgatctg tgggtcgatc tgatagctgg gcgccgcgcc
     3661 ctcccgcagc aggtcgctgg tgaaatagaa cgcgtccggc tggaccacag cgttctcgct
     3721 gtcccaaaac aggacatagc gcgcgaggac accccctgcc agcaccagcg cccccatccc
     3781 catcagcagc agaacaatcg ccccgatctt ttgttttcgc cctctgcggg cgcgtttttt
     3841 cggcatagag gcacctcccc cccgttttta tttcatagat tttatttccc cgccgcttcc
     3901 ggcaaagcct gcactgcccg gatatgcagc gagaccgcgt caacctcccg cgcataggcg
     3961 gcgtccgatt tgtctttcgg ccatgcgact gtcaaagtat aaaggtgatt tacctgcttc
     4021 ccggcgggca gtaagccgtt ctcccaaacc gccgctgcgc catcgttcaa gtccgccaac
     4081 cggccctcgt ttgccgcggg tgttgtcccc acaagagaaa aggtcagcgg cagattgcct
     4141 gtcgtctcca cttccaccac atagctctgc gccacctcgc tgacgacgcc gttttttgca
     4201 ttggcgacgg tgaacgagat cgtcctgctt gtgccggggg ccagaccggc cagggccgta
     4261 gtcagatcca cgttggatgt cccctccatg gcgaccgcgg cgacggcggc gccaccgctg
     4321 ccgcgcaccg tgccggtaaa gcgggaatag gagacgccca ccaccagcgc aaacgcggcg
     4381 ctgaggcaca gcaggtaaac ggcaacgcgc cctacggtca actgtctctt catccgttca
     4441 cagcctctct cttccgccgt tttttcaagc cgcccgacgc ggttcttcgg cccactaccc
     4501 ggcgtgtact ttcagactca cgatataggc ccggtcctct tcgctttgcc cggcccccgc
     4561 cgcggtgtcc gccgcgtcgt cgccgccctc gtacagccat tcccactcga cgccgaggac
     4621 aaggggctcc gcccccaccg tcccgccgcc aagccgtagg gtttgccctt tcttcagggt
     4681 ttgccattcc ccggcgctgc cgtccgcgtc caccagccta aaacgcatgg gcagatgaaa
     4741 gctggcctca gagagagaga tctcatagct gagcgcatcc cggcggctgt tcaccagccg
     4801 gaagctgtag gacccacgcg aaccgggttg gatattgccg tttgccgcag cgccggtgcg
     4861 gttataaaac agatcgacac cgctttcact ctcccacgag gaccagccgc ccacggcgct
     4921 ctgctgcgcc tccagcacgc ccttgtcgtc gtcctccggc tcggaggaat ccggcggcag
     4981 gctgggcgtg ggggtgggtt ccaggctgga tgtggggccc ggcgtcggcg ccggagcgga
     5041 ggacgagccg tcgccgccat ggccctggga ggaggacgtt cccccgtcac cgccggaccg
     5101 cgagggctgc gagcccgcac ttccgccctg gcccgggcgg ccctgcgtac cggcctgccc
     5161 gttggggctg gggcggggcg tctgcccccc ggcggcgatc tgatagggct tttccggcga
     5221 gaggacggcg ccgtccggcg tgcggattcc ctccgccgag atcaccgtgc cgtccgccag
     5281 ctccacccgg ccatccggca aaattttctc gccgccgcct gtcgttgtcc caccggtcgg
     5341 gatcggggtc accatattgt ccggcaaaat cagatacccc ggcgccacca ccgtgctgtc
     5401 tttcaagatc acggtgccgg agggcaggac gatctcgcct gcagcagccg tcaagctgct
     5461 tttgtccaca aagaccgttc cgtcatcctg cagcgccacc gcggccccgg gcagcagcac
     5521 caacgccgct ttctccccgc gcagctccac cgccagctct ataaagcgaa cctcggtgga
     5581 gagttccacc gtgtaccggc cctccgccgt ctcggcaacc tccagcggct ggctgccccc
     5641 gcggcccacc gcgatctcgc aggctgccag cactgcgccc tgtgcgtcca ccactgaaag
     5701 cgtgtgatct cccagctcaa cgctgtcgta gaaaaagcgc ccctgcgcgt cggtatccgt
     5761 gcggcgcggc tcgctgcgca gctccaccgt gccgcctgtg tagggggtgc cgtcagcgta
     5821 gcgcacctgg cccgcaagat ggaccgtgcg gctttggtcc ggcccaatga cgatggtgtc
     5881 aatgatctgt cccagcgccg ggccgctgtt gcgcccaatt aaaaagcccg cgataaccga
     5941 cgaaaacagt gccagcaatg ccagtagaat cagcatagcc tcgcgcgccc gttttttgtt
     6001 tttcgctccc cccgccgagg gcgcgcgccc ggtctcgtct tgtggttcca tcgctcacgt
     6061 cctccttttg cagatcgttc tgtcgcggac tgttttcgca gactgtcttt aaagaccctt
     6121 cttcagccct ttgcaaagcg cggcgccggc ccgaccgcgc tttgcgaagg gctgaaaagc
     6181 cctcgccatc cccgcccggc acaggggcgg gatatgtatc gctcttaagc ggtggggatc
     6241 acctgcgtca caacgcactt gatctccagt tttgcctcgg ccaacgcgga cgcgctgccc
     6301 agcgcggtat ctgcggcgtc tgtgctgctg ggattccatc tccaatggag gtagaaagtt
     6361 tgggcattgg cgcccaccac aaccgctgtc tccgtcgggg ccagatcggt cgccgctttc
     6421 cagttttgct cctctgccgg ggccgcttct gtcgcggtga tcgcgtattg aagcggaatt
     6481 ttgtcgatat ttgtttccgt gatcgtaaaa tcaggcttga tggcgacctc acccttattg
     6541 gtcagctcaa tttttacctt gccgctggtg cccggcgcca caacagccga gctggcgctg
     6601 acattgttgc tgttataggt cttgctgaac agggtgctga gatcgatcgt ctttatttcg
     6661 gtcttctcgg tcactccgtt taagtcaaat gaaaactttg ccacggtagc cgttgcgctg
     6721 ccggtcacgt ccgccgtgta ctttgccagc gttccgccca tcaggcaggt gctgatcagc
     6781 gtcagggcca gggccagaac gcccagacgg cctatcgctt ttttcttcat gaggggttcc
     6841 tcctttaaga tagggtcggg aagcgcgaga tggactgcgg aacgcgcttc ccagttgccc
     6901 gcagcgtttt tgccgggtgg ccccggcttt atgggacact tgcggtatac tgtgcctagg
     6961 ccgcaaatga taccatctga ttcatgttcg tcctgtatca tcattctgat aagtatatgg
     7021 gcagacctgt gggataaaat tgcgctgccg aagcagcgtg caggctttgc attgctctgt
     7081 ctcaagcagc tctcagctgc ggtgctcgtt ttctccagca acgccctgcg cagcccgcag
     7141 cgcnnnnnnn nnntttcaac tccgcttcca gcgcatccgc tcgtgccttt tcgttcttat
     7201 ccgtctttcg ccgggtgacg atatcccaga cgatcagcag caagatcggg cagacgataa
     7261 acagaagcat ccccgtggtg ctggagagaa agaggacggc gtttcctgcg ccgccgatgc
     7321 gtccgccctt ccatatcccc tgcacctgct ggggcagcac cggctcggcg tcctcggcgt
     7381 tgttggcgtc gccttttgtg atatagcgcg tcgcgccgcc gccgtcccgc tccacgcgca
     7441 cgatgcggtg ggtcaccgcc tgccccgaga caagataaca aatcacgtcc ccctcctgta
     7501 ggctggcagg gtcggttttg tgaagaaaga taaggtcccc cgtcataaag gtgtcctcca
     7561 tcgagccgga gagcacagcc accggtttga cgccgaacgc gccggggatc tcgtcgggat
     7621 gcagataggt gttggcgatg atgatcacgt tcaccaggat caccgggaag aaaagcacac
     7681 agagcaaaac gctgacaacg cccgcggccc tgcctgatct ttttcttttt tctttcatag
     7741 ggcgtcatcc ccctcttgaa gtctgaactg taagccgcat aagggcaaag gcggcttccc
     7801 tccttgctca aagaccacag ggcaccgcct cttttcgccc ctgcccttgg atccggcggg
     7861 gcgaccgcct ccaccacata atttgtatta tgtggtgggt catgcgctcc cctcgatcag
     7921 ccccaatcgc gcaatcagcc ttttctcttc cctgccgctg gtcacggtgt agattctggt
     7981 ggtctccacg ctggaatgcc ccagcacgtc ggccagccgc acgatgtctt tttgcaggcg
     8041 gtagtacgtc agggcgaaca gatgccgcag attgtgtgga aagacctttt ttctttccac
     8101 ccgcgcctca cggcacaggc gcttcatctc cgcccagata ttgctgcgat ccagcgcgcg
     8161 gccgctgcgg gtgacaaaga cggggccggc tgtgatgcgg cagtgggcgc agtaggcctg
     8221 cagttgtttt acgagctcgg gaggcaaaaa tacgaggcgc tcttttccct tgttcctcac
     8281 gcggacgctt cccttgcgaa gcgcctccac cgtgacgaaa cgatgttcgc tcacccgcag
     8341 ccctgcgccg cacagcgtct gcatcaacag gtacaggcgt tcattttttt tgctgcgcgc
     8401 aacctttaga agacggcggt attcctcttt tgagagctct ttctccgcct gccggaaatt
     8461 agaacgctgt atcttgaaca gcttcacccg gcagtcctgc ctgccgagaa aggccagaaa
     8521 acggttgacg gccaccagca tcgaattcgc gctgctggcc ttatagcttt gcgccaggcg
     8581 ggccttgtac gcgatcacct gctctttgca caattgcttg tcccgcggca aaaaccggca
     8641 aaaagcctct gcgtcccgca tatatttctc gatcgtccgg gggctgcgtt cctcgcagat
     8701 caggcagctc tgaaacctgt ttagcgcctc cctgttccac acactgtttt tcatcctcaa
     8761 acctccctt
//
LOCUS       DBMDYA010000024         8710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2172_length_8710_cov_1.572192, whole genome shotgun sequence.
ACCESSION   DBMDYA010000024 DBMDYA010000000
VERSION     DBMDYA010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_8710_cov_1.572192"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="ACZPFK_01175"
     CDS             <1..222
                     /locus_tag="ACZPFK_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404950.1"
                     /translation="NTYYGDILCDALPAEAWLGERGFRKAVLHYRLELPLGETMALAR
                     GRAQYHGAPCWYVEGVRDGKRCFEGNLLF"
     gene            263..826
                     /locus_tag="ACZPFK_01180"
     CDS             263..826
                     /locus_tag="ACZPFK_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HIR1404951.1"
                     /translation="MELHEVLKGSPIIAAVKDYEGMRKALSCDSEVFFILFGTICDIG
                     DIVDEVKAAGKIVFVHLDLIEGLAGKDVAVDFLLKTTKLDGIISTKPALLKYARGKGL
                     LTVQRFFLLDSIAAANVDRQFATSKPDMVEVLPGLMTSAIAEIAREHPDTPVIAGGLI
                     KTKEDVVNALNAGAAAVSSTNHDVWFM"
     gene            954..1484
                     /locus_tag="ACZPFK_01185"
     CDS             954..1484
                     /locus_tag="ACZPFK_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1404952.1"
                     /translation="MAFHIERMTPATQRGKGLAHYQAWRQTYRGLMPDAYLDAQSPEE
                     SIRRAALLPDTTLVALEGARVVGFVCYGPEAREFAGRPGASEIMALYLLREAQGRGLG
                     RALMEAALRLLPHREVVLFALESNERAIGFYRHMGFCLTGRELRQETGFGELVELEML
                     LTRPDGWEPQGEEDLR"
     gene            1493..2143
                     /locus_tag="ACZPFK_01190"
     CDS             1493..2143
                     /locus_tag="ACZPFK_01190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1404953.1"
                     /translation="MELKTKRLLLRPLSDEALAARLAEENDPGMRAALEEMLAGSRAQ
                     PGQRLWHTLWEMAQQQGGAAVGSFCFHGAPMNGAVEIGYGVEAEFRGRGYAQEAVRAA
                     ANWAFGQQDVYWLEAEAAPDNASSLHLLEKLGFVRCGEGEEGPRFSLEKPAPANSALF
                     MCLFTGLGISFGVSFHHLGLGLSFGAAAGLVLGTALDAADRQRRAELKALREGRST"
     gene            complement(2662..3765)
                     /locus_tag="ACZPFK_01195"
     CDS             complement(2662..3765)
                     /locus_tag="ACZPFK_01195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR1404954.1"
                     /translation="MERVLILVVDGCAPGYLNEATAPGLYRLAGECGFAKRIQCAMPS
                     VTNVNHACILSGQWPRETGVIGNYFYDPRTGREGFVEERGYMKAPTILQRCRAAGGRT
                     ALLTVKGKVLGVYGDGADIGLSAEAPCEETLRRYGLKAPPPIRSVESTEWILQAALRC
                     VERDAPDLVYCTTNDYVFHHCPPGHPHAVAQIRAVEDYVEKIHRADPGRQIYITADHG
                     MNQKTVIVNFQTAAERAGFRLFCLPPLKDRYVENHIYQEGGMLYVFLKDPGRGADFAA
                     FARRHPQVEKVLTAREAAEAYHLPEGQIGDFVLFSAEGCAFGEVEGEILHTEASRTHG
                     SLYEREIPLIAIHPAAGAEAYAYNKDIAAYLAL"
     gene            complement(3799..5172)
                     /locus_tag="ACZPFK_01200"
     CDS             complement(3799..5172)
                     /locus_tag="ACZPFK_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR1404955.1"
                     /translation="MSESATILAAQRRYFNSGATLPLPGRRAALKRLLNAVTAREEEI
                     LAALAQDLGKARMEGYLTEVGIVKSELNAMLRHLDRWAKPRRVGTPLAHFPSRSMVWP
                     EPYGVVLVQSPWNYPFQLCLAPVVGALAAGNCVMIKPGSAAPATAAVIAGLIADAFAP
                     EYVHVLRLGGDSHEEILAEHYDYILFTGSQRVGSIVMEAAAKHLTPVTLELGGKSPCI
                     VDETADLRLAAKRIAFGKFLNAGQTCVAPDHLLVQRSVKAPLLAELEKAVAELYGPDP
                     LHSPDLPKIVNEHHFRRLLGLMEGQQVAVGGRWDEKARKIAPTVLDNVSDDAAVMGEE
                     IFGPILPVLTFETLDEVVRRQHALPKPLALYLFTRSKAAERRIIENVSYGGGCVNDTI
                     VHLANDRLPFGGVGASGMGSYHGKASFDTFTHYKGVLKKSNLLDIPLRYAPYSEKAFR
                     LAKKIMR"
     gene            complement(5274..5960)
                     /locus_tag="ACZPFK_01205"
     CDS             complement(5274..5960)
                     /locus_tag="ACZPFK_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HIR1404956.1"
                     /translation="MIPLSIATAAYLAQLSLLLWVAYSDAQFHRYTAAKAVTSAGFLL
                     VALLARMIGPRTMGPRFWLLFSAMVLCAAGDVLLGLANNGKGVHSKTFLKGLVCFGLA
                     HVVFCAFYAAKTPPGWYDLLLPLALLGITMALSKGGLLRLKKLKRPAFCYCFLVGLMC
                     SMAVSSVVVAGFRGPANLFAAAGSILFLLSDCIIIFLYFYIRQNRWMRFANLTAYYLG
                     LLLLALSAAF"
     gene            complement(5957..6691)
                     /locus_tag="ACZPFK_01210"
     CDS             complement(5957..6691)
                     /locus_tag="ACZPFK_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1404957.1"
                     /translation="MVDKKSPVPLYIQLRDTLREAVGAGEYPAGSQLPTEFELARRYG
                     LGRATVRSALDLLLRDGVIEKRHGVGTFVRRPRREFSFEPLISLTSFLRGLGVPTENR
                     VLKKRWLRAEAGLAAKARVPEGARLGGVLRLRYALGEPIALEQSYFAEDIYQRLKALP
                     LESSLSQLLLRDLAVDVQRIEQTIIRRQPTAAECRALALSGEQRVMELTRWLYRAGEQ
                     TPFYYIEFIVTAGAYEDIAATGEGAR"
     gene            6835..8571
                     /locus_tag="ACZPFK_01215"
     CDS             6835..8571
                     /locus_tag="ACZPFK_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013434.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1404958.1"
                     /translation="MSQTDLGRVRRALRKAGLDGVSVGELNGSLLLEGALPAWADVVR
                     AGAVAAAHRGGFPHVVNHLDVPGLPPVEPKLPCLQDKALEGARPDVAVIGGGVTGCAI
                     ARELRRYDLSVLLLEKEADLAMGASSRNDGMIHPGIDLKAGTLKHRYGIAGNAMFDQL
                     AAELELPFVRNGSYIIFSAAWERLLIPAFYHRARKGHIPGVRYVPRGELLRLQPEVAG
                     WAKGAIYTGSSGILCPYGLTVALAENAVQNGARVSLLTAVTGIERRGDRVAALHTNRG
                     TLYPRLVVDAAGVWADRIAAMAGDEFFTIHPRKGSSSILDKKAGYLTASVLAKAPFAD
                     VKQHTKGGGIVRTIDGNILVGPTAQEVRRREDNSTDQPQIDASFEKHSKVAPHLSERD
                     VITYFSGTRAATYEEDFIVGPSPRVRNLLYAAGIQSPGLTAAPAIAKDIAALAVQALS
                     DEGPVPLNIHFDPHRPAIPRPAEMTAEARQALIERDPDFGEIVCRCEGITKGEILAAL
                     RRPVPADSIDGVKRRVRAGMGRCQGGFCSPVVAQLISQERGIPLWQVRKSGEGSEIGF
                     GPTKGGERNGTV"
     gene            8558..>8710
                     /locus_tag="ACZPFK_01220"
     CDS             8558..>8710
                     /locus_tag="ACZPFK_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1404959.1"
                     /translation="MERFDVLVLGGGPAGLAAALAAEEQGAAVCLLEREARLGGILKQ
                     CIHDGFG"
BASE COUNT         1454 a         2908 c         2957 g         1391 t
ORIGIN      
        1 aatacctatt atggggacat cctctgcgac gccctgcccg cagaggcctg gctgggggag
       61 cgcggctttc gcaaggccgt cctccactac cggctggagc tgccgctggg cgagacgatg
      121 gccctggccc gcggcagggc gcagtatcac ggcgcgccct gctggtatgt ggagggcgtg
      181 cgggacggca agcgctgttt cgagggcaat cttttgtttt gagcccggcc ccggccggga
      241 gagggaaaag gaggcgcaag gcatggaact gcacgaggtt ttaaagggct cgcccatcat
      301 cgcggcggtc aaggactacg aggggatgcg caaggcgctc agctgcgaca gcgaggtgtt
      361 tttcatcctc ttcggcacca tctgcgatat cggcgacatc gtggacgagg tcaaggccgc
      421 gggcaaaatc gtctttgttc acctggacct gatcgagggg ctggcgggca aggacgtggc
      481 ggtggatttt ctgctcaaga ccaccaagct ggacggcatc atctccacca agccggcgct
      541 gctcaagtac gctcgcggca aggggctgct gacggtgcag cgcttttttc tgctggactc
      601 gatcgccgcc gccaatgtgg accggcagtt cgccaccagc aagccggaca tggtggaggt
      661 gctgcccggg ctgatgacct ccgccatcgc cgagattgcg cgggagcacc cggacacccc
      721 ggtcatcgcc gggggactga tcaaaaccaa ggaggatgtg gtgaacgcgc tgaacgcggg
      781 cgcggcggcg gtgtcctcca ccaaccacga cgtgtggttc atgtaggcgt tggccccgcg
      841 ccgcctctgc ggcgcggggc ctgtttcgca tcagccgctg cggcgggggc cccaactgcg
      901 cgcggcgccg cataggcgcc gcccggccgg gcaacagaag aggaggggcg tcgatggcgt
      961 ttcacattga gaggatgacc ccggccaccc agcgcggcaa ggggttggcg cattatcagg
     1021 cctggcggca gacctaccgc ggcctgatgc cggacgccta tctggacgcc cagtcgccgg
     1081 aggagagcat ccgccgggcg gcgctgctgc cggacaccac cctcgtcgcg ctggaggggg
     1141 caagggtggt ggggtttgtc tgctacggcc ccgaggcgcg ggagtttgcc gggcggcccg
     1201 gcgcgtccga gatcatggcc ctctacctgc tgcgggaagc gcagggccgg gggctgggac
     1261 gcgccctgat ggaagcagcg ctgcgcctgc tgccccaccg tgaggtggtg ctgttcgcgc
     1321 tggagagcaa cgagagggcg atcggctttt accggcatat ggggttttgc ctcaccggcc
     1381 gggagctgcg ccaggagacc ggcttcggcg agctggtgga gctggagatg ctgctgacgc
     1441 gcccggacgg ctgggagccg cagggggagg aagatctgag atgaaagccc ctatggaact
     1501 gaagacaaag cgtctgctgc tgcgcccgct gagcgacgag gctctggcgg cccggctggc
     1561 ggaggaaaac gaccccggca tgcgcgcggc gctggaagag atgctggcgg gcagccgggc
     1621 ccagcccggg cagaggctgt ggcacaccct gtgggagatg gcgcagcagc agggcggcgc
     1681 ggccgtgggc agcttctgct ttcacggcgc gccgatgaac ggcgcggtcg agatcggcta
     1741 cggcgtggag gcggagttcc ggggccgggg ctacgcgcag gaggccgtca gggccgcggc
     1801 caattgggct tttgggcagc aggacgtcta ctggctggag gcggaggccg cgccggacaa
     1861 cgcctcctcg ctgcacctgc tggaaaagct ggggtttgtt cgctgcggtg agggcgagga
     1921 gggaccgcgc ttttcgctgg aaaaaccggc ccccgccaat tcggcgctct tcatgtgcct
     1981 gtttaccggc ctggggatca gcttcggcgt ctctttccac catctggggc tggggctcag
     2041 cttcggcgct gcggccgggc tggtgctggg gacggcgctg gacgcggccg accgccagcg
     2101 ccgcgccgag ttgaaagccc tgcgggaggg acgctccacc tgagcgcccc tcttttttgc
     2161 gccggggggc aggaggcgtc gaaaataggc cgcgccgaat gtgaatcata tcgaaatagg
     2221 cacgctggaa atgggccgcg ccgaatgtga atcacgtcgg aggcgggcta cgcgcgaagc
     2281 aggtcacgct ggaagtgcgt cgcgcctgaa gtgggctgcg cccaaagtgg agtgcgccgg
     2341 aaatgggtcg cgcgggcggt agcccacatc ggaaaaaggt cgcgctggag gcagattgcg
     2401 ccggaggcac gcgtcctttc tctttgcggc cggaggcagg cggggcgcgg gcgctctgat
     2461 cttttttccg tcgcccggcg tgaaagcggc ataaaaaagc aggatcgcgg aatttttccg
     2521 tgatcctgct tttttatgcg atgaaacgcc ttatgggggc actttctccc ccggcgggcc
     2581 aactgaggca tcttgaaaag ccgcctgccg cccgcgcagg gcagggccgg aacgcacacg
     2641 gcctgcggtg ctttcggctg ctcacagcgc cagatatgcc gcgatgtcct tgttgtacgc
     2701 gtaggcctcc gccccggctg cggggtgaat ggcgatcagc gggatctccc gctcgtagag
     2761 cgagccgtgg gtgcgggagg cctcggtgtg caaaatctcc ccctccactt cgccgaaagc
     2821 gcatccctcg gcggaaaaca gcacaaaatc gccgatctgc ccctcgggca gatgataggc
     2881 ctccgccgcc tcccgggcgg tgaggacctt ttccacctgc ggatgccggc gcgcaaacgc
     2941 cgcaaagtcc gctccccggc cggggtcttt cagaaagacg tagagcatgc cgccctcctg
     3001 gtagatgtgg ttttccacat agcggtcttt caggggcggc aggcagaaca gccgaaaacc
     3061 cgcgcgttcc gcagccgtct gaaaattcac gatcaccgtc ttctggttca tgccgtggtc
     3121 cgccgtgata tagatctgcc gcccggggtc cgcgcggtga atcttttcca catagtcctc
     3181 caccgcccgg atctgggcga cggcgtgcgg gtggccgggc gggcagtggt gaaaaacata
     3241 gtcgttggtg gtgcagtaga ccaggtctgg ggcgtcgcgt tccacgcagc gcagagcggc
     3301 ctgcaggatc cattcggtgc tctccacact ccggatggga ggcggcgctt tcaggccgta
     3361 ccgccgcagg gtctcctcgc atggcgcttc ggcgctgagc ccgatatccg cgccgtctcc
     3421 gtacaccccc agcaccttgc ccttcaccgt cagcagggct gttctgcccc ccgccgcccg
     3481 gcatcgctgc agaatggtgg gggctttcat atagccccgc tcctccacaa atccctcgcg
     3541 gccggtgcgg gggtcgtaaa aataattccc gatcaccccc gtctcccgcg gccattggcc
     3601 ggacaggatg caggcgtggt tgacattggt gaccgagggc atggcgcact ggatgcgctt
     3661 tgcaaacccg cactcccccg ccagacggta caggccgggg gccgtcgcct cgttcagata
     3721 acccggggca cagccgtcca ccaccaggat caagaccctc tccatcccac acgcttcctc
     3781 tctcgcgggc ctctggggtc accgcatgat tttttttgcc agccggaacg ccttttcgct
     3841 gtagggcgca tagcgcagcg ggatgtccag caggttcgac tttttcagca cccccttgta
     3901 atgggtgaac gtgtcgaagc tggccttgcc gtgatagctg cccatgccgg aggcgcccac
     3961 cccgccaaag ggcaggcggt cgtttgccag atggacgatg gtgtcgttga cgcagccgcc
     4021 gccgtagctg acgttctcga tgatcctgcg ctccgcggcc ttactgcggg tgaacaggta
     4081 gagggccagg ggctttggca gcgcgtgctg acgccggacc acctcgtcca gcgtctcaaa
     4141 ggtcagcacc ggcagaatgg ggccgaagat ctcctccccc atcaccgccg cgtcgtcgct
     4201 gacgttgtcc agcacggtgg gggcgatctt gcgggccttt tcgtcccagc ggccccccac
     4261 cgccacctgc tggccctcca tcaggcccag caggcgcctg aaatggtgct cgttcacgat
     4321 cttgggcagg tccgggctgt gcagcgggtc ggggccgtag agctcggcca ccgctttctc
     4381 cagctccgcc agcagagggg ccttgacgct gcgctgcacc agcaggtggt cgggcgcgac
     4441 gcaggtctgc cccgcgttga ggaatttgcc gaaagcgatg cgctttgccg ccagcctcag
     4501 atccgccgtc tcgtccacga tgcaggggct cttgccgccc agctccagcg tgaccggggt
     4561 caggtgcttt gccgcggcct ccatcacgat gctgcccacc cgctgcgagc cggtgaacag
     4621 gatgtagtca tagtgctcgg ccaaaatttc ctcgtggctg tcgccgccga ggcgcagcac
     4681 atggacgtat tccggcgcaa aggcgtcggc gatcagcccg gcgatcaccg ccgccgtggc
     4741 cggggcggcg ctgcccggct tgatcatcac gcagttgccc gcggccagcg cgcccaccac
     4801 cggcgcgaga cagagctgga acgggtagtt ccaggggctc tgcaccagca ccacgccgta
     4861 gggctcgggc cagaccatgc tgcgggaggg gaaatgcgcc agcggcgtgc ccacccggcg
     4921 cggcttggcc cagcggtcaa gatggcgcag catggcgttc agctcgcttt tgacgatgcc
     4981 cacctcggtg aggtagccct ccatcctggc cttgcccagg tcctgggcca gggcggccag
     5041 gatctcctcc tcgcgcgcgg tgacggcgtt gagcagacgc ttcaaggcag cccgccggcc
     5101 gggcagcggc agcgtcgccc ctgagttgaa atagcggcgc tgggccgcca ggatcgttgc
     5161 ggattcagac atggtctttc cctcctgtga agagcctgcg gccgcccgac cggggcgcgt
     5221 aaaagggggc tgcacgggca gccgtcccgg tatccctgcc gggggcggcg cccttaaaac
     5281 gccgccgaca gcgccagcag cagcaggccc agataatagg cggtcaggtt ggcaaaacgc
     5341 atccagcggt tttgccggat ataaaaatag agaaagatga tgatgcagtc ggacagcaaa
     5401 aacaaaatgc tgcccgcagc cgcgaacagg ttggccgggc cgcgaaagcc cgccaccacc
     5461 accgagctga ccgccatgct gcacatcagc cccaccaaaa agcaatagca aaaggccggg
     5521 cgcttgagct ttttcagccg cagcagcccc cctttcgaca gcgccatggt gatgcccagc
     5581 agcgccagcg gcagcagcag gtcgtaccag cccggcggcg ttttggccgc ataaaaagcg
     5641 cagaacacca catgggccag cccaaagcag accagccctt tcaaaaatgt tttcgagtgg
     5701 acgcccttgc cgttgttggc gagccccagc agcacatccc cggccgcgca gagcaccatg
     5761 gcggaaaaaa gcagccaaaa gcgcggcccc atcgtccgcg ggccgatcat ccgggccagc
     5821 agcgccacca gcaaaaagcc ggcgctggtc accgccttgg cggccgtgta gcggtggaac
     5881 tgcgcgtcgg aataggcgac ccacagcagc agcgagagct gggccagata ggccgctgtg
     5941 gcgatggaca gtgggatcat ctcgcgccct cccccgttgc ggcgatatcc tcataggccc
     6001 ccgccgtgac gatgaactcg atgtaataaa agggcgtttg ctcccccgcc cggtagagcc
     6061 agcgggtcag ctccatcacc cgctgctccc cgctcagggc cagcgcccgg cactcggccg
     6121 ccgtcggctg ccgccggatg atggtctgct cgatgcgctg gacatccacc gccaaatcgc
     6181 gcagcagcag ctgtgagagc gagctctcca gcggcagcgc tttcaaccgc tggtagatat
     6241 cctcggcaaa atagctctgc tccagcgcga tcggctcgcc cagggcgtac cgcagccgca
     6301 gcacgccgcc cagccgcgcg ccctcgggca cccgcgcctt ggccgcaagg cccgcctccg
     6361 cccgcagcca gcgctttttc agcacccggt tctcggtggg cacccccagc ccccgcaaaa
     6421 acgaggtcag cgagatcagc ggctcaaagc tgaactcccg ccgggggcgg cgcacaaagg
     6481 tgcccacccc atggcgcttt tcgatgacgc cgtcccgcag cagcaggtcc agcgcgctgc
     6541 gcaccgtggc ccggcccagg ccgtagcggc gggccagctc aaactcggtg ggcagctggc
     6601 tgcccgcggg atactccccc gccccgacgg cctcgcgcag ggtgtcgcgc agctggatat
     6661 acagcgggac cgggcttttt ttgtcgacca cggcaaaagc ctcttttcac gaaagattta
     6721 caaaaccgcc tcttgcatcc tgttgacttt attgtaaaga aaagctacaa tgatgtcaag
     6781 atgtccggac aaccgaacca catgtttgtc cccagctcaa aggagggaac cgcattgtca
     6841 caaactgatc tcggccgggt gcgccgggcc ctgcgcaagg ccgggctcga cggcgtgtcg
     6901 gtgggcgagc tgaacggcag cctgctgctg gagggcgcct tgcccgcctg ggccgacgtg
     6961 gtgcgcgcgg gcgccgtcgc cgccgcccat cgggggggct ttccccacgt ggtcaaccac
     7021 ctggacgtgc ccggcctgcc cccggtggag cccaagctgc cctgcctgca ggacaaggcg
     7081 ctggagggcg cgcggccgga cgtggcggtg atcggcggcg gcgtcaccgg ctgcgccatc
     7141 gcccgcgagc tgcgccgcta tgacctcagc gtgctgctgc tggaaaaaga ggccgatctc
     7201 gccatgggcg cctccagccg caacgacggc atgatccacc ccggcatcga cctcaaagcc
     7261 ggcactctga agcaccgtta cggcatcgcg ggcaacgcca tgttcgacca gctggccgcc
     7321 gagctggagc tcccctttgt gcgcaacggc agctacatca tcttttccgc cgcctgggag
     7381 cggctgctga tccccgcctt ttaccaccgc gcccgcaagg ggcatatccc cggcgtgcgc
     7441 tatgtgcccc gcggcgagct gctgcgcctg cagcccgagg tcgccggctg ggccaagggc
     7501 gcgatctaca ccggctcctc gggcatcctg tgcccctacg ggctgaccgt cgccctggcg
     7561 gaaaacgcgg tgcagaacgg ggcccgcgtc tcgctgctca ccgccgtcac cggcatcgag
     7621 cgccggggcg accgggtggc cgccctacat accaaccgcg gcaccctcta cccgcgcctg
     7681 gtggtggacg ccgccggggt ctgggcggac cgcatcgcgg cgatggccgg ggacgagttt
     7741 ttcaccatcc atccccgcaa gggcagcagc agcatcctgg acaaaaaggc gggttatctc
     7801 accgccagcg tgctggcaaa ggcccccttt gcggatgtga agcagcacac caagggcggc
     7861 ggcatcgtgc gcaccatcga cggcaatatc ctggtgggcc ccaccgcgca ggaggtgcgc
     7921 cgtcgggagg acaactccac cgaccagccg cagatcgacg cctcctttga aaagcacagc
     7981 aaggtggccc cccacctgtc cgagcgggac gtgatcacct atttttccgg cacccgcgcg
     8041 gccacctatg aagaggactt catcgtcggc cccagccccc gggtgcgcaa tctgctgtac
     8101 gcggcgggca tccagagccc cggcctcacc gccgcgcccg ccatcgcaaa ggatatcgcg
     8161 gccctggcgg tacaggccct ctctgacgag gggccggtgc cgctcaacat ccactttgac
     8221 ccccatcgcc cggcaatccc ccgcccggcc gagatgacgg cggaggcgcg gcaggcgctg
     8281 atcgagcgcg acccggattt cggcgagatc gtctgccgct gcgagggtat caccaagggc
     8341 gagattctgg cggcgctgcg gcggccggtg cccgcggaca gcatcgacgg ggtcaagcgc
     8401 cgggtgcgcg ccggcatggg gcgctgccag ggcggctttt gcagcccggt ggtggcccag
     8461 ctgatctcgc aggagcgggg catccctctg tggcaggtgc gcaagagcgg cgagggcagc
     8521 gagatcggct ttggccccac caagggaggc gagcggaatg gaacggtttg acgtgctggt
     8581 gctgggcggc ggcccggcgg gcctggccgc ggcgctggcc gcggaggagc agggcgcggc
     8641 cgtctgcctg ctggagcggg aggcgcggct cggcggcatc ctcaagcagt gcatccacga
     8701 cggctttggg
//
LOCUS       DBMDYA010000025         8566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2210_length_8566_cov_1.368104, whole genome shotgun sequence.
ACCESSION   DBMDYA010000025 DBMDYA010000000
VERSION     DBMDYA010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2210_length_8566_cov_1.368104"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            274..2964
                     /locus_tag="ACZPFK_01225"
     CDS             274..2964
                     /locus_tag="ACZPFK_01225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1404960.1"
                     /translation="MQKRIIPAILAVALAVSIFLSVLSIHDLQGNARVINYAGVVRGA
                     TQRLVKQELSGNPNDGLIEKLDGILEELATGEGENGLHRMEDKTFQDLVAQMQSKWGQ
                     VKEGILQVRAGGDRARLYEESEAFFDLADSTVFAAEAYSERQVKNAERTLLLLGAVFL
                     LLCAGLAWTQARRRRALREAEEKNRAESEKLARMTEDLRAPMDEISELMYVADLQTHD
                     LLFLNEAGRKTFGVTGGLPGKKCYEVLQGRDSPCEFCSTPFLVPGENYTWEFTNPLTQ
                     KHYLLKDRLLQWEGRAARLEIAFDITEAEAEKQALKNTLDAEQAMIECVRTLYQEHDV
                     ARSFPLVLQRMGAFLEADRVCLSTVQDGALHCDFEWGREGAEPQINLLKELPWGTAER
                     WQPLLSRRGCLVLEEIESLRETAFEDYRALHAAGVHSMVVAPLEQDGRLRGVLVIYNA
                     PAGRMSSIAPVLQTLCYFLVLTYDRTQSEQQLSQLSYYDTLTSFYNRNRYIEDAQALS
                     DYPGSVGIVYLDVNGLKDINDCQGHAAGDQALVECARQMRETFQEADFYRIGGDEFVI
                     ICPGIERDVFEARTAALRLRFERAEHCRAAVGAKWAQCFDDVRQVIAEADAKMYEDKK
                     TFYRRNPVSHRYRHLSDEVLHLSDPAALEEEIRRDRFVVYLQPKVSSADRAAVGAEAL
                     VRYRSNSGSLMLPGNFLPMLEESRTISQIDFYVFEYACSRLQEWAARGKESLPIAVNF
                     SRSSLSQPDFVPRLVALCEEYGVERKYLEIELTETAHEEDGVDLKSLIHALRKQGFVV
                     SIDDFGTEYANLALLSTVDFDVLKLDKSLVDDVAHSYKARTVVENIVNICGKMDISVV
                     AEGIETEEQLEALRGCGVKLVQGFLFSRPIPIEEYEAKYL"
     gene            3247..4560
                     /locus_tag="ACZPFK_01230"
     CDS             3247..4560
                     /locus_tag="ACZPFK_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1404961.1"
                     /translation="MKPNTKNFAGGMGAFFALWSTQAFSTLGSAMTNFALIVWSYQQQ
                     GSALTTALLSVCSYAPYVLVSIFAGAASDRWDKRRTMLAADGFAALCTAAVLMLLRAG
                     GLRIWHLYLLNALNGLMNSVQQPASAVAVSLLTPRRQYQRVSALQSLSNSLVTVLTPA
                     LATALLTLVSLEAVLLFDLVTCAVAVGTLGLFVRIPASPAQAGRETVLESARRGLAYL
                     WSHRGVLHLILFLAAINLTASMYNAALPAMLLSRAGGGEKVLGLVNTLTGLAALAGSV
                     AVSLLPAPKSRVRAICNALLFSMSTENLLLALGTSAPVWYLGAVLGWLFIPVMNTNLD
                     ALLRVTIPVEMQGRVYAARNTLQFFTIPLGYALGGVLVDQVFEPLMAGQAGAGPLTAL
                     VGSGKGSGAALLFLFLAALGVATCLVFRADGQLWALEGRPVSRKK"
     gene            4755..>5600
                     /locus_tag="ACZPFK_01235"
     CDS             4755..>5600
                     /locus_tag="ACZPFK_01235"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR1404962.1"
                     /translation="MKKWMVRAGSVLLFFAAINDYALSVWAGRRFSGWGFALLALLFL
                     AANLRLPKKGAPSRRIEVLDSGCELLKLFLAVTAAEIVFSILFFLFCRPLNAANVLLH
                     LLAVLVFENILFWNGMLRVYLTSVQLGIKWRVIGALCGWIPLVHLWALGRILRITGRE
                     VAFERAKLELNAVRAESEVCATRYPVLLVHGVFFRDSKVLDYWGRIPAELKRNGAAVY
                     YGGQQSAASVADSGEELAARIHNLVKETGCEKVNLIAHSKGGLDCRYAIACAGAGELV
                     ASLTTV"
     assembly_gap    5601..5610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5611..6096
                     /locus_tag="ACZPFK_01240"
     CDS             <5611..6096
                     /locus_tag="ACZPFK_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HIR1404963.1"
                     /translation="VNTPHRGCLFADELLGRIPVGVQNAIARKYNRAAWLLGDREPDF
                     LRAVRDLTASACARFNEEVKDLPGVLYQSVGSVMRRAASGKFPLNMTYPLVRKFDGES
                     DGLVAVESMPWGASFQLVRTEKRGVSHADMIDLNRENIPGFDVREFYVQLVRGLRDRG
                     L"
     gene            6176..7198
                     /locus_tag="ACZPFK_01245"
     CDS             6176..7198
                     /locus_tag="ACZPFK_01245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1404964.1"
                     /translation="MATITLGRTGLRVEQNGFGALPIQRISKSEAVRLLRRAQEGGMT
                     YFDTARAYTDSEEKLGAAFDGLRDKVAIATKTMAKSAEAFWDDLHTSLRQLGTDYVDV
                     YQFHTPPFCPKPGDESGLYDAMLQAKRQGKVRFIGITNHRLDVAHEAVSSGLYDLLMF
                     PFSYISSEKELAVADACLEKGMAFVAMKALCGGLITDSAAACAWVARRPGVLPIWGIQ
                     RERELDEFLSYIPTPPQLDEGLRAVIERDRRELQGDFCRGCGYCMPCPQGIQINSCAR
                     ASVMMRRAPAINFLSEAGQEMMKKIEDCIGCGQCAAKCPYGLDTPALLQRNYQDFKEV
                     LAGKPL"
     gene            complement(7287..8240)
                     /locus_tag="ACZPFK_01250"
     CDS             complement(7287..8240)
                     /locus_tag="ACZPFK_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016114.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIR1404965.1"
                     /translation="MTDAQIAKSQTMIEIAKLYYLDGMSQDEISRQLHMSRSNISRLL
                     AKSVEAGIVQITINDSRFIHLDVAHRIGKKYGLQDVIIVNSHSDAERTRRKAGEAVSG
                     YLVERVQNGMLLGVTRGKIIYYASRATHNTRGIRTDAVQLMGCTVNSSPSQDSYMLTE
                     TFAQRLSGIGYVMPVPLMSKSKQLRDQLLQEPLCGSVVEKFSSVDIALLEIHSLRVAL
                     SSQFREPWLTKADSLQLSEVDAIASVCGRYFDPRGLPCSAGINDRVIAAGYDQLKAVP
                     IRVGTAIGANMLHSTLAVLRSKVLTALIIDESLAFDLDSQA"
BASE COUNT         1600 a         2396 c         2831 g         1729 t
ORIGIN      
        1 caagccacga gcgtgcctgt gccaccgaca ttacgggagg acattcctcg ccgagaagca
       61 tacacgtttc ggtgagatta ggggttataa catctgcggc ttgtaatggc ggggaacaaa
      121 gtgctataat ggacgcaaag caatctgtct tttttgcacg cctttggcca tctgcgggcc
      181 gggcgtattt ttgtaacggg gttgtgagcc gggcggaccg ggacgacgcc tgttttttca
      241 gggcgacttc attgttttaa aggcgggctt atcatgcaaa agcggatcat accggcgatc
      301 ttggccgtgg cgctggcggt ttctattttt ctctcggtcc tttccatcca cgatctgcag
      361 ggcaacgcgc gggtcatcaa ctacgccggt gtggtgcgcg gcgcgaccca gcgcctggtc
      421 aaacaggagc tcagcggcaa cccgaacgac gggctgatcg aaaagctgga cgggatattg
      481 gaggagctgg ccaccggcga gggagaaaac ggcctgcacc ggatggagga caagaccttt
      541 caggacctgg tggctcagat gcagagcaaa tggggacagg tgaaagaggg catcctccag
      601 gtgcgcgcgg gcggggaccg cgcgcggctc tatgaggaga gcgaggcctt ttttgacctg
      661 gcggacagca cggtctttgc cgcggaggcc tactcggagc ggcaggtgaa aaacgccgag
      721 cggaccctgc tgctgctggg ggccgtcttt ttgctgctgt gcgccgggct ggcgtggacg
      781 caggcgagac ggcggcgcgc cctgcgggag gcggaggaga agaaccgggc ggagagcgaa
      841 aagctggcgc gcatgaccga ggacctgcgc gcccccatgg atgagatctc agagctgatg
      901 tacgtggccg acctgcagac ccacgaccta ctttttctca atgaggcggg tcggaagacg
      961 ttcggtgtga cgggcggcct gcccggtaaa aagtgttatg aggtgctgca ggggcgggac
     1021 agcccctgcg agttttgctc cacgccgttt ctggtgcccg gtgaaaatta tacctgggag
     1081 ttcaccaacc ccctcaccca aaagcattat ctgctcaagg accgcctgct gcagtgggag
     1141 gggcgcgccg cccggctgga gatcgctttt gatatcaccg aggcggaggc ggaaaagcag
     1201 gccttgaaga acacgctgga cgcggagcag gccatgatcg aatgtgtgcg caccctctat
     1261 caggagcacg atgtcgcccg ctcctttcca ctggtgctgc agcggatggg cgctttttta
     1321 gaggctgacc gggtctgcct ctccacggta caggatgggg cgctgcactg tgattttgag
     1381 tggggcaggg agggcgcaga gccgcagata aacctgctga aagagctgcc ctggggcacg
     1441 gcggagcgct ggcagcccct gctcagccgg cgcggatgcc tggtgctgga agagatcgaa
     1501 agcctgcgcg agaccgcttt tgaggattac cgggcgctgc atgcggcggg tgtgcacagc
     1561 atggtcgtcg ccccgctcga gcaggacggc aggctgcggg gcgttttggt gatctacaac
     1621 gcgccggcgg ggcggatgag cagcattgcg cccgttttgc agacgctgtg ctattttctt
     1681 gtgctcacct atgaccgcac acaaagcgag cagcagctct cccagttgag ctattacgac
     1741 accctcacct ctttttataa ccgcaaccgg tatatcgagg atgctcaggc cctctccgat
     1801 tacccgggct cggtgggtat cgtctatctg gatgtcaatg gattgaaaga catcaatgac
     1861 tgccagggcc acgcggcggg cgaccaggcg ttggtggaat gtgcgcgcca gatgcgggaa
     1921 acctttcagg aggccgattt ttaccgcatc ggcggcgacg agttcgtcat catctgcccg
     1981 ggcatagagc gggacgtgtt cgaggcgcgc acggcggcgc tgcgcctgcg ctttgagcgg
     2041 gcggagcact gccgcgccgc ggtgggggca aaatgggcgc agtgctttga cgacgtgcgg
     2101 caggtcatcg ccgaggcgga cgcgaagatg tatgaggaca aaaagacctt ttaccgccgc
     2161 aaccccgtct cccaccgcta tcgccatctc agcgacgagg tgctgcatct gtccgacccg
     2221 gcggcgctgg aggaggagat ccgcagggat cgctttgtgg tctatctgca gccgaaagtc
     2281 tcgtcggccg accgcgctgc ggtgggggcg gaggcgttgg tgcgctaccg ctccaattcc
     2341 ggctcgctga tgctgccggg caatttcttg cccatgctgg aggagagccg caccatcagc
     2401 cagatcgatt tttatgtctt cgagtacgct tgctcccgcc tgcaagagtg ggcggcgcgg
     2461 ggcaaggagt ccctccccat cgcggtcaat ttttcccgca gctcgctctc ccagccggat
     2521 tttgtccccc gcctggtggc cctgtgcgag gaatacggcg tggagagaaa atatctggaa
     2581 atcgagttga ccgagaccgc gcatgaagag gatggggtcg acctgaagtc gctgatccat
     2641 gccttgagaa agcaggggtt tgtggtctcc atcgacgatt tcggcacgga gtatgccaat
     2701 ctggccctgc tctccaccgt ggatttcgac gtgctcaagc tggacaagag cctggtggac
     2761 gacgtggccc acagctataa ggcccgcacg gtggtggaaa atatcgtgaa tatctgcggc
     2821 aagatggata tctcggtggt ggcggagggg atcgagaccg aggagcagct ggaggcgctg
     2881 cgcgggtgcg gcgtaaagct ggtgcagggc tttttgttca gcaggcccat ccccatcgag
     2941 gagtatgagg caaaatacct ctagggcaaa agcgcgtgcg aaagagaggg ctttttcttt
     3001 ttgcacggca ggcgcgaatg tgatagaata gaggcagcag agcggcccgc gggatgcgcg
     3061 ggccgctctg ctgctttcag gaggccgaag agagaggggg ctgggccatg aggctttgca
     3121 ttgacgccgc tccatgcagg gtctgatccc ggacgggaca gcatggagag cgggataact
     3181 cccgtccggc aggctttgca tgtttaagga tccgaccgat tggaaaatta aaggagcaaa
     3241 gtcaaaatga aaccgaatac aaaaaatttt gccggtggca tgggggcgtt tttcgccctc
     3301 tggtccaccc aggctttttc cactttgggc agtgcgatga cgaattttgc cctcatcgtc
     3361 tggtcgtatc agcagcaggg ctcggcgctc accaccgcgc tgctctcggt gtgctcttac
     3421 gcgccctatg tgctggtgag catcttcgcc ggggcggcca gcgaccggtg ggacaaaagg
     3481 cggaccatgc tggcggccga cggctttgcc gcgctttgca cggcggcggt gctgatgctg
     3541 ctcagggccg gcgggctgcg catctggcat ctctacctgc tcaacgccct gaacgggctg
     3601 atgaactcgg tgcagcagcc ggcgtccgcc gtggcggtga gcctgctcac cccgcggcgg
     3661 cagtaccagc gggtgagcgc gctgcaatcg ctgtccaact ctctggtcac ggtgctcacg
     3721 cccgcgctgg ccacggcgct gctgaccctt gtctcgctgg aggcggtcct tttatttgac
     3781 cttgtcacct gcgccgtggc ggtggggacg ctgggcctgt tcgtccgcat cccggcttct
     3841 cccgcacagg cggggcggga gaccgtgctg gaaagcgcgc gccgggggct tgcttatctg
     3901 tggtcgcacc ggggcgtcct gcacctgatc ctttttctcg ccgccatcaa tctgaccgcc
     3961 tcgatgtaca atgccgccct gcccgccatg ttgctctcac gcgccggggg cggggagaaa
     4021 gtgttggggc tggtgaacac cttgaccggc ctggccgcgc tggcgggcag cgttgcggtg
     4081 tcgctgctgc cggcgcccaa aagccgggta cgggccatct gcaacgcgct gctcttttcc
     4141 atgagcacgg aaaacctgct gcttgcgctg ggcacaagcg cgcccgtctg gtacctcggg
     4201 gcagtgctgg gctggctgtt catcccggtg atgaacacca acctggacgc gctgctgcgg
     4261 gtgaccatcc cagtggagat gcaggggagg gtctatgcgg cgcgcaatac cctgcaattc
     4321 ttcaccatcc ccctgggcta cgccctgggc ggggtactgg tggaccaggt ctttgaaccg
     4381 ttgatggcgg ggcaggcagg cgcaggaccg ctcaccgcgc tggtgggcag cggcaaggga
     4441 tccggcgccg cgctgttgtt cttgtttctg gcggcgctgg gggtggccac ctgcctggtc
     4501 tttcgggcag acgggcagct ctgggcgctg gagggacgcc ccgtttcccg caaaaagtaa
     4561 gtgcaaaggg taaaaaagaa aagttttgag aaaaaacagc ctcctttccg gccgcggcgc
     4621 ctctcggcgc cccccggcgg ggaggccgtt ttgttgttca cctgcgtgcg cgcagcgcag
     4681 tggccttgcg ttcgttttgc ctgtttcttt ttggctttgt ccgtggtaac ataaaaagaa
     4741 ctgggggagt gaatgtgaaa aaatggatgg tgcgggccgg atcggtcctg ctcttttttg
     4801 cggctatcaa tgactatgcg ttgtcggtct gggctgggcg gcggttcagc ggatgggggt
     4861 tcgcgctgtt ggcgctgctg tttttggcgg cgaatctgcg cctgcccaaa aaaggcgcgc
     4921 cctcgcgccg catcgaggtg ctggacagcg gctgcgagct gctgaagctg tttctggccg
     4981 tcaccgccgc ggagatcgtt ttttccatcc tgttttttct gttttgccgg ccgctgaacg
     5041 ccgcaaatgt cctgctgcat ctgctggccg tactggtgtt tgagaatatc ctgttctgga
     5101 acgggatgct gcgggtctac ctcacctcgg tgcagttggg catcaagtgg cgggtgatcg
     5161 gcgcgctctg cggctggatc cccctggtcc acctctgggc gctgggccgc atcctgcgca
     5221 tcaccgggcg cgaggtggct tttgaacggg ccaagctgga gctgaacgcc gtgcgggccg
     5281 agagcgaggt ctgtgcgacc cgatatcctg ttttactggt gcacggcgtt ttttttcggg
     5341 attccaaggt cctggattac tgggggcgca tcccggcgga gctcaagcgc aatggggcgg
     5401 ccgtctatta cggcgggcag cagtccgccg cttcggtggc ggacagtggg gaagagctgg
     5461 cggcgcgcat ccataacctt gtcaaggaga ccggctgtga aaaggtgaat ctgatcgccc
     5521 attccaaggg cggcctcgat tgccgctacg ccatcgcctg cgcgggcgcg ggggagctgg
     5581 tggcctcgct gaccaccgtc nnnnnnnnnn gtcaacaccc cgcaccgggg ctgccttttt
     5641 gcggatgagc tgctggggcg catccccgtc ggggtgcaga atgccatcgc ccgcaaatac
     5701 aaccgggcgg cctggctgct gggggacaga gagccggatt ttctgcgcgc ggtccgcgat
     5761 ttgacggcct cggcctgtgc gcgtttcaac gaggaggtca aagacctgcc cggcgtcctt
     5821 taccagagcg tcggctcggt gatgcggcgc gcggccagcg gcaaattccc actcaacatg
     5881 acctatccgc tggtgagaaa gtttgatggg gaaagtgacg gcctggtggc ggtggagtcc
     5941 atgccctggg gggcctcttt ccagctggtg cgcacggaga agaggggcgt ttcccacgcg
     6001 gacatgatcg acctcaaccg ggaaaatatc ccgggctttg acgtgcgcga attttatgtg
     6061 cagctggtgc gcgggctgcg ggatcgaggg ctctgagacg gcgcccgcgg attgccgccg
     6121 cgtccgccgc gctttggaaa taaaaaacag gaagatgaaa aaaggaggag agcacatggc
     6181 aacgatcaca ttgggccgca ccggcctgcg ggtggaacaa aacgggtttg gggcgctgcc
     6241 catccagcgc atctcaaaaa gtgaggccgt ccgcctgctg cgcagggcac aggagggcgg
     6301 tatgacctat tttgacaccg cccgggccta tactgacagc gaagaaaagc tgggcgcggc
     6361 ctttgacggc ctgcgcgaca aggtggccat cgccaccaag acgatggcga aaagcgcaga
     6421 ggcgttttgg gatgatctgc acaccagcct gcggcagctg ggtaccgact atgtggatgt
     6481 ctatcagttc cacacaccgc ctttctgccc caagcccggg gacgaaagcg gcctctacga
     6541 cgccatgctg caggcaaagc ggcagggcaa ggtccggttc atcggcatca ccaaccaccg
     6601 actggatgtg gcccacgagg cggtcagctc tggtctctat gatctgctca tgttcccgtt
     6661 cagctacatc agcagcgaaa aagagctggc cgtcgccgac gcctgtcttg aaaaggggat
     6721 ggcttttgtc gcgatgaaag cgctctgcgg cggcctgatc acagattcgg cggccgcctg
     6781 cgcctgggtg gcccggcgcc cgggcgtgct gccgatctgg ggtatccagc gcgagcggga
     6841 gctggacgaa tttttgtcct atatccccac gccgcctcaa ttggatgagg ggctgcgcgc
     6901 ggtcatcgag agggaccggc gggagctgca aggggatttc tgtcgcggct gcggctattg
     6961 catgccctgc ccgcagggga tccagatcaa ctcctgcgcc cgcgcctcgg tcatgatgcg
     7021 ccgcgccccg gccatcaatt tcctctctga ggcggggcag gagatgatga aaaaaatcga
     7081 ggactgcatc ggctgcggcc agtgcgcggc gaaatgcccc tacggcctgg acacccccgc
     7141 gctgctgcag cggaattatc aggactttaa agaggtcctg gcaggcaaac ctctgtgacg
     7201 cgcagaggat gttgacgagg agtaaagggc ccggctccct gtttttttgc aggggccggg
     7261 ccctttttaa ttgtaggtca gagcgctcaa gcctgggagt ccagatcaaa cgctaaggat
     7321 tcgtcaatga tcagcgcggt gagcactttg ctgcgcagca cagcgagggt ggagtgcagc
     7381 atatttgccc cgatggcggt gcccacgcgg atggggactg ccttgagctg gtcatagcct
     7441 gccgcgatga cgcgatcgtt gatcccggcg ctgcagggca gcccgcgcgg atcgaaatag
     7501 cgcccgcaga ccgaagctat ggcgtccacc tcgctgagct gcagagaatc cgccttggtc
     7561 agccatggct cccggaattg ggaggaaaga gccacccgaa gcgaatgaat ctccaacaaa
     7621 gcgatatcaa cagaagaaaa cttttcaacg acgctgccgc ataagggctc ctgcagcagt
     7681 tgatctctga gctgttttga cttggacatc agaggcaccg gcatgacgta gccgattccg
     7741 ctcagacgtt gcgcaaaggt ttcagtcagc atatagctgt cctgtgaggg ggagctgttc
     7801 actgtgcagc ccatcagttg cacagcgtcc gtgcggatgc cccgggtatt gtgggtggcg
     7861 cgggatgcgt agtaaatgat tttgccgcgg gtgaccccca gcaacatgcc gttctgcacc
     7921 ctttccacca ggtaaccgga cacggcctct cccgctttgc ggcgggtgcg ctctgcgtcg
     7981 gaatgagaat taacgatgat gacatcctgc aggccatatt ttttgccgat gcggtgggcg
     8041 acgtctaaat ggataaaacg ggaatcattg atggtgatct ggacgatgcc cgcctcgacc
     8101 gatttggcaa gcaggcgcga gatgttggag cggctcatgt gcagctggcg agagatctca
     8161 tcctgcgaca tcccgtctaa ataatacagc tttgcgattt cgatcatcgt ctgtgatttg
     8221 gcaatttgtg cgtcagtcat ggatcacctc gcggaggaaa gaaaaaaggg aaaaacaaaa
     8281 atcggcttgc ggcgacggga gaaacggtaa aaaaacctgg gcaatcggcc tgtgagcttg
     8341 aaaaagcctg agtgagacga gagctgccgc cgctttgctt gaggaaaaac agagaagaaa
     8401 aatttaatat cttgcgcttt tcttttttaa attatagcgc tctgtcttga aaaagaacaa
     8461 gcctaaaatg cagttttgcc gctgccttgt ttttccagtc ttgcatatgc ttcggcaaac
     8521 tccctcacac agcgctcttt ggtataggtt tgatacagct ccatgg
//
LOCUS       DBMDYA010000026         8555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2213_length_8555_cov_1.723918, whole genome shotgun sequence.
ACCESSION   DBMDYA010000026 DBMDYA010000000
VERSION     DBMDYA010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_8555_cov_1.723918"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /locus_tag="ACZPFK_01255"
     CDS             complement(<1..2297)
                     /locus_tag="ACZPFK_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1404966.1"
                     /translation="MERPIWCDKEIVGKNALPVPGCGFPCASLKEALAGREDAFALTL
                     NGDWAFLFCPSVDACPQGFERPEFDDVNFDRLPVPSNWQLHGYDTPIYSNTRYPLPIS
                     TKRAELPSIDPARNPVGLYRRRFSVPPAFAGRRVLLQLDGVNSAAEVWCNGRFAGFCL
                     SSFDAHRFDLTGLLAPGEDLLAVKVYRWSAGSYLEDQDMWRLAGIFRDVWLVAEPEAG
                     IRDLAVKTLFPGGYDSAVLDLTVDLPQTPMENAFLQVVLLDPQGAPALTREEPLAAGG
                     PVHLACPVAAPALWSDELPALYRLAVTLVQGGAALDARGLWVGFREVRADGDRLLLNG
                     RPIELRGVNRHEFHPSCGHAVTPELNEADIRLLKQNNLNALRTSHYPGSRALYDLCDR
                     YGVLVMSECNLETHGLAKLLPRSDPGWTAHCLDRMEKMVTLLKNHPSILFWSLGNESY
                     MGDCFVQMRRRAAELDDTRLFHYEPDTTFKAADVYSQMYATVAHVERIGKGQPVFLSR
                     CSYTSLGRRAGAKTYGHRPYLLCEYAHCMGNSLGNFADYWRVFRRYERLAGGFIWDFA
                     DQALFRNGRWCYGGDFGDQPNDGPFALNGILRADRSPNPALWEVRQVLAPFELSLSDE
                     GARIKSLLRFACAKTLALRWSLCEDGVPFESGELPVPPLEPGAAARLEIGYGGREAAG
                     AVDLVCVLTDTAETPFAAAGRTLCGASLRLQERRPDPPGEWVPVQKEKRELVLSNGRC
                     TARILRRDGTLASFTVDGTELLSSPL"
     gene            2555..3136
                     /locus_tag="ACZPFK_01260"
     CDS             2555..3136
                     /locus_tag="ACZPFK_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009988217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1404967.1"
                     /translation="MSKMTWRGGTLLAPVPPALVSCGTLEEPAVLTVAWTGTLNTRPP
                     RTYVSIRPERNSYPMIKQRGEFVINLAPAALARAVDLCGCKSGRAADKFALAHLTPAA
                     ASAVSAPLIAECPVSLECRVFQVVPLGSHDMFLADIVAVDVEEALVDAAGKLHLDKAD
                     LLAYAHGEYFRLGAKVGSFGFSVRKKKKGRAGK"
     gene            complement(3597..4370)
                     /locus_tag="ACZPFK_01265"
     CDS             complement(3597..4370)
                     /locus_tag="ACZPFK_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR1404968.1"
                     /translation="MYQVRNFTDNDDVRVLDQKGPFSVLEYQRDLSVTPASAVTAYYC
                     AQMNVRKRQLSCDLSRAPVTVQAGAMQWMLGNVSANTGVKGVGDLFSKAVRGKVTGES
                     AIKPEYTGNGLLVLEPTYRHLILSDAADWGGSVILDDGLFLACDANLKHKAVMRSNFS
                     SAVAGGEGLFNLSLNGRGVFCIEADCPKEELVEIILENDVLKIDGNFAIAWSGSLEFT
                     VERSGKSLIGSAASGEGLVNVYRGSGRVLMMPTAKMPNI"
     gene            4855..5892
                     /gene="hrcA"
                     /locus_tag="ACZPFK_01270"
     CDS             4855..5892
                     /gene="hrcA"
                     /locus_tag="ACZPFK_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1404969.1"
                     /translation="MAMDERKQRVLAAIVSLYAAGGEPVGSNLLQQHFDMAVSSATLR
                     NEMAALTKLGLLEQPHTSAGRVPSMAGYRYYIDHLMSADGRRLQPPPRERRAIDEAFD
                     NFDYDPERLAKATAKELAKLTGFTAVATTPKSEDVKVAYYSVLQVGRCSAAVLSVSSV
                     GGVATRVAVTKAPIAAAALAEVASLLNRTLCFVAKDDAGQEMQQALWDALGEDKNLWP
                     VVAAAVRLLQGAGRGKVFVEGEQNLLSWPEIEPNLHGCLDLLNRHDELQRLVVPKTDR
                     PMVLLGDDMPGYRMPGVAIVARRYLAGGGLTGAIALMGPSRMNYDKVIPTLEYFAAKL
                     CQAMTGTGKEE"
     gene            5899..6537
                     /gene="grpE"
                     /locus_tag="ACZPFK_01275"
     CDS             5899..6537
                     /gene="grpE"
                     /locus_tag="ACZPFK_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1404970.1"
                     /translation="MEEEIKKEDGLQQEPQAGPETAAGAEAGGAAEPQAPEAAEKKED
                     RKKEKKERKAAAALEKELGAVKERLAGVEKSLAGAKETLMRTAAEYDNYRKRTAREKD
                     ATFNNGVTFAVTQLLPVVDTLEMAAAAPTEDENYKKGVEMTLTKCVEAFGKLGIQEIE
                     ALGKPFDPELHSAVMQDPATDEAPSGTVTRVLQKGYQLDGKVVRHAAVAVAE"
     gene            6649..8496
                     /gene="dnaK"
                     /locus_tag="ACZPFK_01280"
     CDS             6649..8496
                     /gene="dnaK"
                     /locus_tag="ACZPFK_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1404971.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGEPVVIANAEGSRTTPSVVAFSK
                     TGERMVGQVAKRQAITNPERTVASIKREMGTDYKVNIDGKAYTPQEISAMILGKLKAD
                     AEAYLGEKVTEAVITVPAYFNDAQRQATKDAGAIAGLDVKRIINEPTAASLAYGIDKE
                     KDQKILVYDLGGGTFDVSIIEMGDGVTEVLATAGDTRLGGDDFDQRVIDYLAEEFKKE
                     NGIDLRSDKMAAQRLKEAAEKAKIELSGVASTAINLPFITADATGPKHLDTTLTRAKF
                     DELTADLVEKTMVPTRQALKDAGLSPSELSKVLLVGGSTRIPAVQEAVKKFMGTDPFK
                     GINPDECVAIGAAIQGGVLGGDVQGILLLDVTPLSLGIETYGGVCTRIIERNTTIPTQ
                     KSQIFSTAADNQPSVEVNVLQGEREFAKDNKSLGTFHLDGIPAAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGKEQSITITASSNMSKDDIEKAVKEAEQYAAEDKKRKEEVETRN
                     AADAMVYQTEKTLTDMGDKMSEDEKKAITEAKDALKEALKGNDMAKIKSEQEALEKAV
                     SAMSQRVYSEAAQAQQAQGAAGAADAAGGKADDGVVDADYREVKDDDNK"
BASE COUNT         1702 a         2727 c         2848 g         1278 t
ORIGIN      
        1 aggggggagg acagcagctc cgtcccgtcc accgtgaagc tggcgagggt gccgtcccgg
       61 cgcaggatcc gcgccgtgca gcggccgttg ctgagcacca gctcccgttt ttctttttgc
      121 accggcaccc attcgccggg cgggtcgggc cgccgctcct gcaggcgcag gctggcgccg
      181 cacagcgtcc ggcccgccgc ggcaaagggc gtctcggcgg tatctgtcag cacacagacc
      241 aggtccaccg ccccggcggc ctcccgcccg ccgtagccga tctccagccg cgccgcggcg
      301 ccaggctcca gcggcggcac cggcagctcg ccgctctcaa agggcacgcc gtcctcgcac
      361 aggctccagc gcagggccag cgtttttgcg caggcaaagc ggagcaggct cttgatccgg
      421 gcgccctcgt cggagaggga cagctcaaac ggcgccagca cctgccgcac ctcccagagc
      481 gccgggttgg ggctgcggtc ggcgcgcagg atgccgttga gggcaaaggg gccgtcgttg
      541 ggctggtcgc cgaagtcccc gccgtagcac cagcggccgt tgcgaaacag cgcctggtcg
      601 gcgaagtccc agatgaagcc gccggccagc cgttcatagc ggcggaagac acgccaataa
      661 tcggcaaaat tgcccaggct gttgcccatg cagtgagcgt attcgcagag cagatagggc
      721 cggtggccgt aggtcttggc ccctgcccgc cgccccagcg aggtgtagct gcagcgggag
      781 agaaacaccg gctgcccctt gccgatccgc tccacgtggg ccaccgtggc gtacatctgg
      841 ctgtagacat cggcggcttt aaaggtggtg tccggctcgt agtggaaaag gcgggtgtcg
      901 tccagctccg ccgcacggcg gcgcatctgc acgaagcagt cccccatata gctctcgttg
      961 cccaggctcc agaagaggat ggaggggtgg ttcttcagca gcgtgaccat cttttccatc
     1021 cggtccaggc agtgggcggt ccagcccggg tcgctgcggg gcagcagctt tgccaggccg
     1081 tgggtctcca ggttgcactc gctcatcacc agcaccccat agcggtcgca caggtcgtag
     1141 agggcccggc tgcccgggta gtggctggtg cgcagggcgt tcaggttgtt ctgtttgagc
     1201 aggcggatgt ccgcctcgtt gagctcaggg gtcaccgcat ggccgcagct ggggtgaaat
     1261 tcgtgccggt tcacaccgcg cagctcgatg ggcctgccgt tgagcagcag gcggtcgccg
     1321 tcggcgcgca cctcgcgaaa gcccacccac agcccccggg cgtccaacgc cgcaccgccc
     1381 tgtaccaggg tcaccgccag ccggtaaagg gcgggcagct cgtcgctcca cagcgcgggg
     1441 gcggccacgg ggcaggccag atgcaccggc cccccggcgg caagcggctc ttcccgcgtg
     1501 agggccgggg cgccctgggg gtccagcagc accacctgca gaaaagcgtt ttccatcggc
     1561 gtctgcggca ggtccaccgt caggtccagc acggcgctgt catagccgcc cggaaacagc
     1621 gttttcaccg ccagatcgcg gatgcccgct tccggctcgg ccaccagcca cacatcgcgg
     1681 aaaatgcccg ccagccgcca catgtcctgg tcctccagat agctgccggc agaccagcgg
     1741 tagaccttga cggcgagcag gtcttctccc ggcgccagca agccggtcag gtcaaagcgg
     1801 tgggcgtcaa agctggacag gcaaaagccc gcaaagcggc cgttgcacca gacctcggcc
     1861 gcgctgttca cgccgtccag ctgcagcagc acccggcgtc cggcaaaggc tggcggcacc
     1921 gaaaagcgcc gccggtaaag gcccaccggg ttgcgggcgg ggtcgatgga ggggagctcc
     1981 gctctcttgg tggagatagg cagcgggtag cgggtgttgg aatagatcgg ggtatcatag
     2041 ccgtgcagct gccagttgga gggcaccggc aggcggtcaa agttgacgtc gtcgaactcc
     2101 ggccgctcaa acccctgcgg gcaggcgtcc accgaggggc agaacaaaaa cgcccaatcc
     2161 ccgttgaggg tcagggcaaa agcgtcctcc cggcccgcca gcgcctcttt cagcgacgcg
     2221 caggggaagc cgcagccggg cacgggcagc gcgtttttgc cgacgatctc cttgtcacac
     2281 cagatgggcc tttccatatc cgggtacctc ctgaaaatgc aagcgttgtt ccgcttttca
     2341 ttataacacg gggcagggca gggccgggag aaaaggaaga aaaaatatgc ggtttgtggt
     2401 atgatacagg ggaaaacgcg ggcttttgcc cgccttgccc accccgcggc ccggcaggag
     2461 cgacgggacg gcgcccgcgc cccggcggtg gcaaaggccg ccaccctgcc tggacgcggg
     2521 gacaggacgc ggatataagg gaggaagcgg agagatgagc aaaatgacct ggcgcggcgg
     2581 cacgctgctg gcgccggtgc cgccggcgct ggtgagctgc ggcaccctgg aggagcctgc
     2641 ggtgctgacg gtggcctgga ccggcaccct caacacgagg ccgccgcgca cctatgtctc
     2701 catccggccg gagcgaaatt cttaccccat gatcaagcag cggggcgagt ttgtcatcaa
     2761 cctggccccg gccgcgctgg cccgggcggt ggacctctgc ggctgcaaga gcgggcgcgc
     2821 ggcggacaag tttgccctgg cgcacctcac cccggccgcc gcctcggcgg tgtcggcccc
     2881 cctcatcgcc gagtgcccgg tcagcctgga atgccgggtg ttccaggtcg tgccgctggg
     2941 cagccacgac atgtttttgg cggatatcgt ggcggtggac gtggaggaag ccctggtgga
     3001 tgcggccggc aagctgcatc tggacaaggc ggacctgctg gcctatgccc acggtgagta
     3061 ttttcgcctg ggggccaagg tgggcagctt cggcttttcg gtgcgcaaaa agaaaaaggg
     3121 cagggcgggg aagtgagagg ggagaatggg gcgtcgcagg caggcgcgga cgccgcacgg
     3181 caaaggccgg gcacgccgtc ccggcccatt tcaagaaata gttgttcgac ccacaaaagc
     3241 ccggctgcac ttttgctccg cccgtgaaga agatgtttta ttcaagcgtg ctggggccaa
     3301 aaatatcggg ctggaagcgg cggttttagc ccgcggtgtg cagataaaag aggttggcgg
     3361 tctcccggga ccgccaacct cttttattgt gcatccgccg ccctgcgggc gctcaggatt
     3421 ttagggccgt gggcgggacg tccataggat cgtttttcgg ccattcgttt cggttgccgc
     3481 gcagcggcgg gaaacgagac ctaaaagctt atctctgggg acccgatcgg agaaccggcc
     3541 gatcgaccga aaaaagagcg tttggttctt ttttgtctct cccacgcagc ggggattcag
     3601 atgttgggca ttttggcggt gggcatcatc agcaccctgc cgctgccccg gtagacgttg
     3661 accagtccct cccccgaggc ggcggacccg atcagagatt tgcccgatcg ctctacggta
     3721 aattccaagc tgccgctcca ggcgatggca aaattgccgt cgattttcaa cacgtcgttt
     3781 tccaggataa tctctacaag ctcctctttc gggcaatccg cttcgatgca gaacacgccg
     3841 cgcccgttga ggctcaggtt aaacaggccc tcgcctccgg ccaccgctga ggaaaaatta
     3901 gagcgcatga ccgccttgtg cttcaggttg gcgtcacatg ccaggaacag accgtcgtcc
     3961 aaaatgaccg agccgcccca gtcggcggca tccgacagga tcaggtgccg gtaggtgggc
     4021 tccaacacca gcaggccgtt gccggtgtac tcgggcttga tcgcggattc gcccgtgacc
     4081 ttgccccgca ccgctttgct gaacagatcg ccgacaccct tgacccccgt gttggcgctc
     4141 acgttcccca gcatccactg catggccccg gcctggacgg tgacgggtgc gcgggagagg
     4201 tcgcaggaga gctgcctctt gcggacgttc atctgcgcac agtaataggc ggtgacggcg
     4261 gaggccgggg taacactcag gtcccgctgg tattccagaa cggaaaaagg tcccttttgg
     4321 tccaggacgc ggacgtcgtc attgtctgta aaattcctta cctgatacat tcaaaacacc
     4381 tcattgtctt ttgcgcggac cttaaaataa ggacaatccg ccttgccgcg acgagagccg
     4441 gggatggaaa agcaatcctc ctcctgcttt gcgcctttga gggaagagca ctcgcccctg
     4501 gcccccagcg gtctgccgac ccgcccggca caggggcgaa cccgtccgcg ggacgcagcc
     4561 ccgtctctta taggtaaagt atatcgcatt ccgggcgtgg gcacaagaaa aggtgtgcgc
     4621 tgccgcgggg cgtggcggca gaggtcaaag gccgccgggg ccgccggcac atgagaacgg
     4681 ctgccgcggg gcaaactaac cccgcggttg ggcggcggaa atcctttgtg tctaatttgt
     4741 aaaaattgaa aattttttcc gcaggcgctt gacatccgtc taaattgttg taaaatgaat
     4801 ttagcactca agaacataga gtgctaaaac agaaagacga gagagggtgt gggcatggcg
     4861 atggacgagc ggaaacagcg ggtgctcgcg gccatcgttt cgctgtatgc ggccggcggc
     4921 gagccggtgg ggtccaacct gctgcagcag cacttcgata tggcagtatc ctccgccaca
     4981 ctgcgaaacg agatggcggc cctcaccaag ctgggcctgc tcgagcagcc ccacaccagc
     5041 gcaggccggg tgcccagcat ggccggctac cgctattaca ttgatcatct catgagcgcc
     5101 gacggccggc ggctgcagcc gcccccgcgc gagcggcggg ccatcgacga ggcgtttgac
     5161 aacttcgact atgaccccga gcggctggca aaggccaccg caaaagagct ggcaaagctg
     5221 accggcttca ccgccgtggc gaccaccccc aaaagcgagg acgtaaaggt cgcctattac
     5281 agcgtgctgc aggtgggtcg ctgcagcgcc gcggtgctca gcgtcagctc ggtgggcggg
     5341 gtggccaccc gcgtggcggt caccaaagcg cccatcgccg ccgcggcgct ggccgaggtg
     5401 gcaagcctac tcaaccgtac cctctgcttt gtggcaaagg acgacgccgg gcaggagatg
     5461 cagcaggcgc tgtgggacgc gctgggcgag gacaagaacc tgtggcccgt ggtggccgcc
     5521 gcggtgcggc tgctgcaggg ggcggggcgc ggcaaggtct ttgtggaggg cgagcagaac
     5581 ctgctcagct ggcccgagat cgagcccaac ctgcacggct gcctggacct gctcaaccgg
     5641 cacgacgagc tgcagcggct cgtcgtgccc aagaccgacc ggccgatggt gctgctgggc
     5701 gacgacatgc ccggttaccg catgccgggg gtggccatcg tggcccgccg ctatctggcg
     5761 gggggcggcc tcaccggggc catcgccctg atgggcccct cgcggatgaa ctacgacaag
     5821 gtgatcccca cacttgaata ttttgccgca aaactctgtc aggcgatgac gggcaccggc
     5881 aaggaggagt agcacagcat ggaagaagag atcaaaaaag aagacggcct ccagcaagaa
     5941 ccgcaggcgg ggccggagac cgccgccggg gccgaggcgg gcggggcggc tgaaccgcag
     6001 gcccctgagg cggccgagaa aaaagaggac cgcaaaaagg agaaaaagga gcgcaaggcc
     6061 gcggccgcgc tggaaaaaga gctgggcgcg gtaaaagagc ggctcgccgg ggtggaaaaa
     6121 agcctcgcgg gcgccaagga gaccctgatg cgcacggcgg ccgagtacga caattaccgc
     6181 aagcgcaccg cgcgcgagaa agacgccacg ttcaacaacg gcgtcacctt tgcggtcact
     6241 cagctgctgc cggtggtgga cacgctggag atggcggccg ccgcccccac tgaggatgaa
     6301 aactataaaa agggcgtcga gatgaccctg acgaaatgcg tcgaggcatt cggcaagctg
     6361 ggcatccagg agatcgaggc gctgggcaag cctttcgacc cggagctgca cagcgcggtc
     6421 atgcaggacc ccgccaccga cgaggccccc agcggcaccg tcacccgggt gctgcaaaag
     6481 ggctatcagc tggacggcaa ggtggtgcgc cacgccgcgg tggccgtggc ggaatagcgg
     6541 gcgcgacaac tctcggccct gcggggccgt ttcaatagat cggcacacga aaacacaaga
     6601 gcacaaaaaa catcacaagc tgctttgata caaaaggaga gatagattat gggaaagatc
     6661 attggtatcg acctcggtac gacaaactcc tgcgtcgccg tcatggaggg cggcgagccg
     6721 gttgtcatcg ccaacgcgga gggctcccgc accaccccgt cggtggtcgc gttttccaag
     6781 accggcgagc gcatggtggg ccaggtcgca aagcgtcagg ccatcaccaa ccccgagcgc
     6841 accgttgcct ccatcaagcg ggagatgggt accgattata aagtgaacat cgacggcaag
     6901 gcctacaccc cgcaggagat cagcgccatg atcctgggca agctgaaagc ggacgccgag
     6961 gcctatctgg gcgaaaaggt gaccgaggcg gtcatcaccg tgcccgccta cttcaacgac
     7021 gcccagcggc aggcgaccaa ggacgcgggc gccattgcgg gcctggatgt caagcgcatc
     7081 atcaacgagc ccaccgccgc ctcgctggcc tacggcatcg acaaggaaaa ggaccagaag
     7141 attttggtct acgacctggg cggcggcacg ttcgacgtgt ccatcatcga gatgggcgac
     7201 ggcgtcaccg aggtgctggc caccgccggc gacacccggc tgggcggcga cgactttgac
     7261 cagcgcgtca tcgactatct ggccgaggag ttcaaaaagg agaacggcat cgacctgcgc
     7321 agcgacaaga tggccgcgca gcgcctgaaa gaggccgctg agaaagccaa gatcgagctg
     7381 tccggcgtgg cctccaccgc catcaacctg ccctttatca ccgccgacgc caccgggccc
     7441 aagcacctgg acaccaccct cacccgggcg aagttcgacg agctgaccgc cgacctggtg
     7501 gaaaagacca tggtccccac ccggcaggcc ctcaaggacg cgggcctctc gccctctgag
     7561 ctgagcaagg tgctgctggt gggcggctcc acccgcatcc ccgccgtgca ggaggcggtc
     7621 aagaagttca tgggcaccga cccgttcaag ggcatcaacc ccgacgagtg cgtggccatc
     7681 ggcgcggcca tccagggcgg cgtgctgggc ggcgacgtgc agggcatcct gctgctggac
     7741 gtcaccccgc tgagcctggg catcgagacc tacggcggcg tgtgcacccg catcatcgag
     7801 cgcaacacca ccatccccac ccaaaagagc cagatcttct ccaccgcggc ggacaaccag
     7861 cccagcgttg aggtcaatgt gctgcagggc gagcgcgagt tcgccaagga caacaagtcc
     7921 ctgggcacgt tccacctgga cggcatcccg gcggccccgc gcggcgtgcc gcagatcgag
     7981 gtcacctttg acatcgacgc caacggcatc gtccacgtct ccgccaagga cctgggcacc
     8041 ggcaaggagc agagcattac catcaccgcg tccagcaaca tgagcaagga cgacatcgaa
     8101 aaggcggtca aggaggccga gcagtacgcg gccgaggaca agaagcgcaa ggaagaggtc
     8161 gagacccgca acgcggccga cgccatggtc taccagaccg aaaagaccct caccgacatg
     8221 ggcgacaaga tgagcgagga cgagaaaaag gccatcaccg aggccaagga cgctttgaaa
     8281 gaggcgctca agggcaacga catggcgaag atcaagtctg agcaggaggc cctggaaaag
     8341 gccgtcagcg ccatgagcca gcgggtctac agcgaggccg cccaggccca gcaggcgcag
     8401 ggcgccgcgg gcgctgcgga tgcagccggc ggcaaggccg acgacggcgt ggtggatgcg
     8461 gattaccgcg aggtcaagga cgacgacaac aaataacagc cgctgacggc tgagaacgaa
     8521 ccgggcgcgg ctggccgcac ccgcagaaga aaaac
//
LOCUS       DBMDYA010000027         8501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2230_length_8501_cov_1.489286, whole genome shotgun sequence.
ACCESSION   DBMDYA010000027 DBMDYA010000000
VERSION     DBMDYA010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_8501_cov_1.489286"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="ACZPFK_01285"
     CDS             <1..204
                     /locus_tag="ACZPFK_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404972.1"
                     /translation="RALLLEEEPHCTKEELEKAIVQITSIKNKSRTGKSHVKEGSAAH
                     TRFSRLIAAMDVALDCLQRAAER"
     gene            531..1694
                     /locus_tag="ACZPFK_01290"
     CDS             531..1694
                     /locus_tag="ACZPFK_01290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1404973.1"
                     /translation="MTKRWRRWMAAMLTLALAVCAAAPMRAADRSGDIADLQKQLEQI
                     KQQLSQLDSTVENAEAKKTALEQQSAVLRQQITLMLGNIEDTKTAVTAKQQEVDGKTQ
                     EIADTDALFQQRLRAMQLTHTSGVLSTLLSVNSFDELLTASTSLSRISQADTELLQLL
                     AEQKSTLEQQKQELDAQLEELLAQQQALEAKQGELAASIRAQDAAIDQAEADKQAKQS
                     EYDATYTAYRAAVDETNAWMSSHYSTDTPYTGDGTLLCPIQEGYFRISSGFGTRDDPF
                     GGGRSEFHNGYDYAGGNGALMGRAIHAAESGVVTKVEYRTTGYGLKVVVDHGGGLTTL
                     YGHCSAIYVSVGQQVNRGDVIAAVGDSGNSTAAHLHFSVFQNGVAQDSGAYLG"
     gene            complement(2199..2579)
                     /locus_tag="ACZPFK_01295"
     CDS             complement(2199..2579)
                     /locus_tag="ACZPFK_01295"
                     /inference="COORDINATES: protein motif:HMM:NF046335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR1404974.1"
                     /translation="MEEVCEAVMTVTEEKTARAMGSGDLPVLATPAMIALMENAAMRC
                     AARGLPQGITTVGTRVEVSHDRASAVGVRITARATLLEKTERTFTFRVEAFEGETRIG
                     AGTHTRATVQAERFLAKLNRGGAQ"
     gene            complement(2619..4898)
                     /locus_tag="ACZPFK_01300"
     CDS             complement(2619..4898)
                     /locus_tag="ACZPFK_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1404975.1"
                     /translation="MIYFKGFGEIANRGCNEAIALAQELGHLYIGTEHLLGGILREGT
                     SRAAGALAEQGMTLSGYRDFLAQRVGRGIPTRLSPRDFTVGTMRTLEKAVLKTRWLGE
                     VNTRAEHILTALFPTERCTATAFAAQLGVNLAAVCQSCEMSFLPGPMAGLSPRSPSGG
                     TVRASGKGLERYGRDLTRLAAEGRIDPCFGREQETQRLLEILCRRQKNNPCLVGDAGV
                     GKTAIVEGLALRIAGGEVPPELRHRRLISLDLTGLIAGTKYRGDFEERFKGVLEEVSV
                     DGNCILFIDELHSIIGAGAAEGAIDAASILKPSLARGQLRLIGATTMEEYHKFIEKDS
                     ALDRRFGMIRVEEPTQEAALAILTGLRDRYENFHHVKIGDAALCAAVELSVRYLPAKR
                     LPDKALDLIDEAAACMRVGGQNPNRPMGRKEVAAVLARATGIPVGELSREQQTAMAGL
                     EERLARRVVGQRAAVDALSAAIRRSRAGLAEDSRPTGSFLFLGPTGVGKTELCRALAA
                     EVFGDEKALLRYDMSEYMEKQSVARLIGAPPGYVGYEEGGQLTRAVRERPYSVVVFDE
                     IEKAHPDIYNILLQILEEGCLTENGGRQVSFTSAVVILTGNLGASCFSGGMLGFGSRE
                     KEREERRQAVLAEAKRNFRQELLGRLDEILVFQPLGPEEMRQIAGLQVERLCRRAAKQ
                     KIDLAVGTEVVEHLARLGGSPTAGARGIRRIVERKLENPLSEYILSGKIKRCRKLRAV
                     LRGDEILLEQRQPAAKAAV"
     gene            complement(5241..5498)
                     /locus_tag="ACZPFK_01305"
     CDS             complement(5241..5498)
                     /locus_tag="ACZPFK_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1404976.1"
                     /translation="MSYLRKNVGTCSHSTEVTLTQDGTIEDVVVKGGCDGNLKGIRNL
                     LKGMKAEEAIARMKGVKCGSKGTSCPEQIAFALEEALAAQK"
     gene            complement(5715..6998)
                     /locus_tag="ACZPFK_01310"
     CDS             complement(5715..6998)
                     /locus_tag="ACZPFK_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1404977.1"
                     /translation="MKKRILAVIAAACLLFTGCSGLTESLQRLRSDRLPDAPLADLPY
                     IDPHIETPFEPQVMQFYYGQLASDGERQLYRAYLQFLIDGEGNSVYVKGEFTQEQVAQ
                     ALYALQCDYPQFLTRSWGYNCTYYSTSGGSGLGVELSPVEEQDGSGSHQEELYRALSD
                     TLAQAAGQADVWERQLLLYELLVGGTDYNTGASDTAPLTPGEELPLRARLAHTAYGAL
                     VQRQAVCDGYAYAFQLLCSYAGIPAATVTGMSQRDEGLGESGYVENHAWNLIAYGEEY
                     YYCDPTWDDNGGEYLDADGGRVTSPNSVDGLRPLLPQVLHRYFNLSYEEMAGDHEFSP
                     KFSYPTRTGQARDYYTLRELSAQSPGELERLLAAAFVGKGGQDAGFELRVSYPVDNLT
                     EEIFSRLYVVGVRGTVLVGYTPNDSGCCYIFVYHP"
     gene            complement(7366..>8501)
                     /locus_tag="ACZPFK_01315"
     CDS             complement(7366..>8501)
                     /locus_tag="ACZPFK_01315"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1404978.1"
                     /translation="ELYSAPGRTEIGGNHTDHNNGVVMAAAVNLDIIAVVSRNDSTIV
                     RVKSHGFRDADVVDVSDDKVNEREFGRSSALIRGVAARIRQLGGEVGGFDAYTTSDVL
                     RGSGLSSSAAFEVVIGAVFNGEFNSFRFTPVEVAQISQYAENVYFGKPSGLMDQTASS
                     VGSAITIDFKDPAHPAVEEVAFDLAKYGLSLCITDTKGSHADLTDDYAAIRAEMEQVA
                     AIFGKEVLREVDEAEFFANIPEVREKAGDRAVLRAIHFFADTRRAGQLRDAVRAGDIE
                     RFKALIIDGGHSSFEYNQNAYSIKHPREQGVAVGVALSQHILAGQGAWRLQGGGFAGT
                     IQAFVPGAKLEQYIAAMESAFGKGSCYVISVRNYGAVRVDEKL"
BASE COUNT         1520 a         2869 c         2515 g         1597 t
ORIGIN      
        1 cgggctcttt tattggagga agagcctcac tgcacaaaag aagagctgga aaaggcaatt
       61 gtgcagatca cttcgatcaa aaataaaagc aggaccggaa aaagccacgt caaagagggc
      121 agcgcggccc acacgcgctt ttccaggctg atcgcggcga tggacgtcgc cctagactgc
      181 ttgcaaagag ctgctgaaag atagcaccgg gccgatcgag ccggctgctt tttatgaaag
      241 agtatcgccg gggcagcgcc cctctccccc ctcaatttct atccctcaac ctttttacgc
      301 gcccgggcgt cttatgcccg ggcgtttttt cagtttcctg tatcccgggc cctgcggccc
      361 gcaaggtcgt ttcccccctg ccgttttaca aaattgtaaa accattgttt tttgaaaaag
      421 caccttggcc ttttcccctt ttgcagtttt cacaattgaa taattgattt ttcccgttgc
      481 gcgtgctatg ttaaagaata tcgaaaaaat aatttttatg gggtgatcgt gtgacgaagc
      541 gttggcggcg gtggatggcg gccatgctca cgttggcctt ggcggtctgc gcggccgcgc
      601 cgatgcgcgc cgccgaccgc agcggggata tcgccgacct gcaaaagcag ctggaacaga
      661 ttaaacagca gctctcgcag ctggacagca cggtggagaa cgccgaggcc aaaaagacgg
      721 cgctggagca gcagagcgcc gtgctgcgcc agcagatcac cctcatgctg gggaacatcg
      781 aggacacgaa gacggcggtg actgcaaagc agcaggaggt ggacggcaag acacaggaaa
      841 ttgcggacac cgatgcgctg tttcagcagc ggctgcgggc catgcagctc acccacacct
      901 ccggcgtgct gtccaccctg ctgtcggtga acagctttga cgaattgctc accgcctcca
      961 cctccctctc gcgcatctca caggcggata ccgagctgct tcaactgctg gcggaacaaa
     1021 aaagcacctt ggaacagcaa aagcaggaat tggatgccca gctggaggag cttttggccc
     1081 agcagcaggc gctggaggcc aagcaggggg agctggcggc cagtatccgc gcccaggacg
     1141 cggccattga tcaggcggaa gcggacaagc aggccaagca gagcgaatac gacgccacct
     1201 atacggccta ccgggccgct gtggacgaga ccaacgcctg gatgtcctcg cactacagca
     1261 ccgatacgcc ctatacgggc gacggcactc tgctctgccc catccaggag ggttatttcc
     1321 gtatttccag cgggttcggc acccgcgacg acccctttgg gggcggccgc tccgagttcc
     1381 acaacggcta tgattacgcg ggcggcaacg gcgctttgat ggggcgcgcc atccatgcgg
     1441 cggagagcgg cgtggtcacc aaggtggaat accgcaccac cggctatggg ctgaaggtgg
     1501 tcgtcgacca cggcggcggc ctgaccaccc tttacggcca ttgcagcgca atctatgtat
     1561 cggtggggca gcaggttaac cggggagacg tcatcgccgc ggtcggagat tccggcaatt
     1621 ccacagccgc ccatctgcac ttttccgtct ttcaaaacgg tgtggctcag gactcgggcg
     1681 cctatctggg gtgatttttc cggctttacg aaaaagataa aaggggacgc tgatcacgca
     1741 gcgtcccctt ttcttgcttt ttatcgcttc accgcaaagc ggctgtgtgg gccgtcccgg
     1801 cccatggcct taaatcctga gcgcacaccc acagaataag cggatgcaca ataaaacaac
     1861 ccatcctgac agcccctttt ttcgccgccg cgcggcagcc gaaacagagg gcgtaaaaaa
     1921 caacttattt ccagggccgt ggccgaggac gggccacatg accaataaaa cagcaacggc
     1981 tcttaatggc cgtggcgcag agataagcca catggctgaa aaaagactta tctcgatcgc
     2041 cccggccgga ggaccggccg gatggccgaa aaaaaatagc cgctctgcgg ctatttttat
     2101 cgctccgcga taaaaaacaa cttattttaa tggccgcgtt tctcgccgct gcgcggcaac
     2161 cgaaacggat ggccgaaaaa atagccgtag gctatttttt actgcgcgcc gccgcggttc
     2221 agcttggcga gaaagcgctc ggcctgcacc gtggcccgcg tatgcgtgcc tgcgccgatc
     2281 ctcgtctcgc cctcaaacgc ctccacccga aaagtgaacg tccgctccgt cttttccagc
     2341 agcgttgcgc gggcggtgat ccggaccccc accgccgagg cgcggtcgtg gctcacctcc
     2401 acgcgggtgc ccaccgtggt gatcccctgc ggcagccccc ttgccgcgca gcgcatcgcc
     2461 gcgttttcca tcagcgcgat catcgccggc gttgccagca ccggcagatc cccgctgccc
     2521 atggccctcg ccgttttctc ttccgtcaca gtcatgacgg cctcgcaaac ctcttccatg
     2581 ctctgttctc cccgtccctg taaaaaaatg tggccgcgtc acaccgcggc ctttgcggcg
     2641 ggctggcgct gctccagcag gatctcgtcc ccccgcagca cggcgcgcag ctttctgcac
     2701 cgttttattt taccactcag gatatattcg gacaagggat tttccagctt ccgctccacg
     2761 atgcggcgga taccgcgcgc ccccgccgtg gggctgcccc ccaggcgggc cagatgctcc
     2821 acgacttccg ttcccaccgc aaggtcgatc ttctgctttg cggcgcgccg gcagaggcgc
     2881 tccacctgca ggccggcgat ctgccgcatc tcctcagggc ccagcggctg aaagaccaat
     2941 atctcgtcca gacggcccaa cagctcctga cgaaaattgc gctttgcctc ggccagcacg
     3001 gcctgccgcc gctcctcccg ctccttttct ctgctgccga agcccagcat gccgcccgaa
     3061 aagcaggaag cccccaggtt gccggtgagg atgacgacgg cgctggtaaa gctcacctgg
     3121 cggcctccgt tttcggtcag acagccctcc tccaaaatct ggagcagaat attatagatg
     3181 tcagggtggg ctttttcgat ctcgtcgaac accaccacgc tgtaggggcg ctcgcgcacg
     3241 gcgcgggtga gctggccgcc ctcctcatag cccacatagc ccgggggcgc cccgatcagc
     3301 cgcgccaccg actgcttttc catatactcg ctcatatcgt accgcagcag cgctttctcg
     3361 tcgccgaaga cctccgccgc cagggcccgg cagagctcgg tcttgcccac gccggtgggc
     3421 cccaaaaaga gaaaggagcc ggtggggcgg ctgtcctccg ccagccccgc gcgggagcgg
     3481 cggatcgccg ccgagagggc gtccacagcg gcgcgctggc ccaccaccct gcgggcaagc
     3541 cgctcttcca gccctgccat cgcggtttgt tgctcccggc tcagctcccc caccgggatg
     3601 ccggtagcgc gcgcaagcac cgccgccacc tccttgcggc ccatggggcg gtttgggttc
     3661 tgcccgccca cccgcatgca ggccgccgcc tcgtcgatca ggtccagcgc cttgtccggc
     3721 aggcgctttg ccggcagata ccgcaccgac agctccacag cggcacagag cgcggcgtcc
     3781 ccgatcttca catgatgaaa attttcatac cggtcgcgca ggccggtgag gatggcaagc
     3841 gccgcctcct gggtgggctc ctccacccgg atcatgccga agcgccggtc cagggcgctg
     3901 tccttttcga tgaacttgtg atactcctcc atcgtggtgg ccccgatgag ccgcagctgc
     3961 ccgcgcgcca gcgagggctt caggatgctg gcggcgtcga tcgctccctc cgccgccccc
     4021 gccccgatga tggagtgcag ctcgtcgatg aaaaggatgc agttgccgtc caccgagacc
     4081 tcctccagca cccctttgaa gcgctcttca aagtcgcccc ggtacttggt gcccgcgatg
     4141 aggccggtca ggtccaggct gatcaggcgg cggtgacgca gctcaggggg cacctccccg
     4201 cctgcgatgc gcagggccag gccctccacg atagcggtct tgccgacccc ggcgtcaccc
     4261 accaggcagg ggttgttctt ctgccggcgg cagaggatct ccagcaggcg ctgggtctcc
     4321 tgttcccggc caaagcaggg gtcgatgcgc ccctccgccg caagacgggt gaggtcgcgc
     4381 ccataccgtt ccagcccctt gccagaggcc cgcaccgtgc ccccggaggg ggagcgcggc
     4441 gagaggcccg ccatcggccc gggcaaaaac gacatctcgc agctctggca gaccgccgcc
     4501 aggttcaccc ccagctgcgc cgcgaaagcg gtggccgtgc acctctcggt ggggaacagg
     4561 gccgtcagga tatgctcggc gcgggtgttc acctcgccca gccagcgggt cttgagcacc
     4621 gctttttcca gcgtgcgcat cgtccccacc gtaaaatccc ggggtgaaag gcgggtgggg
     4681 atgcccctgc ccacccgctg tgccaaaaag tcgcgatacc cgctgagcgt catcccctgc
     4741 tccgccaacg cgcccgccgc ccggctggtc ccctcccgca ggatgccgcc cagcagatgc
     4801 tcggtgccga tatataaatg ccccagctcc tgtgccagcg cgatggcctc attgcagccg
     4861 cggttggcga tctcgccgaa gcccttgaaa tagatcatcg ttgcatttgc ccctttgcct
     4921 gaaatgtttg ctctcagtat ggggcgggcg ctttgggttt atgccggggg caaaattggc
     4981 aaatcccgtc aagccggaca gcgccggggc atcggcatcg cttctcgccg cgcaaaggca
     5041 aaaagccgcc cgtttcaccg gtccgctgcg acgggccggg ccggtcgctc acgtcccccg
     5101 gcggcgggcc gccgcgcttt tgccgttggg cctgcgcccc gtgctgtctg cgctgccgtt
     5161 ttgccgcctg caccgcaaaa cgcgctgtaa aagggcaaaa agaaagccgc ccctcaggac
     5221 ggctttcaca ggctcaattt ttacttctgg gccgcaagcg cttcctccag cgcaaaagca
     5281 atctgctcgg ggcaggaagt ccccttgctg ccgcatttca cgcctttcat gcgggcgatg
     5341 gcctcctcgg ctttcatccc cttgagcagg ttgcggatgc ctttcaggtt gccgtcgcag
     5401 ccgcctttta ccaccacgtc ctcgatggtg ccgtcctggg tgagagtgac ctcggtgctg
     5461 tgggagcagg tgccgacatt tttgcgcaga tagctcatgg atagatcctc ctgttttttg
     5521 tttcttcaag gcgggcgcgg ccgggcacgg ccccgctttg cgtccatttt accatagcct
     5581 gtcccaaaaa tgtaccgcct gggggccgct tttcaaaact ttggcgtccc gggcaaaaag
     5641 aaacccgttt tcggcttttt tttcatagct ttgcacaggg agatcggctt ttctccccct
     5701 gtatccccca cctgtcaggg gtgatagaca aagatatagc agcagccgct gtcgttgggg
     5761 gtgtacccca ccagcaccgt gccgcgcaca cccaccacat agaggcggct gaagatctcc
     5821 tcggtcaaat tgtccaccgg ataggacacc cgcagctcaa acccggcgtc ctggccgccc
     5881 ttgcccacaa aggcggctgc gagcagccgc tccagctcgc ccggcgactg cgccgacagc
     5941 tcgcgcaggg tatagtagtc ccgcgcctgc cccgtgcggg tgggatagct gaattttggg
     6001 ctgaactcgt gatctccggc catctcctca taggacaggt tgaagtagcg gtgcagtacc
     6061 tgcggcaaaa gcggccgcag cccatccaca gagttcgggc tggtgacccg cccgccgtcc
     6121 gcgtccagat actccccgcc gttgtcgtcc caggtggggt cgcagtagta atactcctcc
     6181 ccgtaggcga tcaggttcca ggcatggttc tccacatacc cgctctcgcc caggccctcg
     6241 tcccgctgcg acatgccggt caccgtggct gccgggatgc ccgcatagct gcacagcagc
     6301 tgaaaagcat aggcgtagcc gtcgcagacg gcctgccgct gcaccaacgc accataggcg
     6361 gtgtgggcca accgcgcccg caggggcagc tcttcgcccg gcgtcagcgg cgctgtgtcc
     6421 gaggcgccgg tgttgtagtc cgtcccgccg accagcagtt cgtacagcag cagctgccgc
     6481 tcccacacgt ccgcttggcc cgctgcctgg gcgagagtgt ccgacagcgc cctgtagagc
     6541 tcctcctgat ggctgccgga gccgtcctgt tcctccaccg gggagagctc cacccccagg
     6601 ccgctgccgc cgctggtgga atagtaggtg cagttgtagc cccaactgcg ggtcaaaaat
     6661 tgcggataat cacactgaag cgcgtagagc gcctgggcca cctgctcctg ggtgaactcc
     6721 cccttgacgt agaccgagtt cccctccccg tcgatgagga actgcaggta ggcgcggtag
     6781 agctgccgct ccccgtcgga agccagctgc ccataataaa actgcatcac ctgcggctca
     6841 aagggggtct cgatatgagg gtctatatag ggcaggtcgg cgaggggcgc gtccggcagg
     6901 cggtcgctgc gcaggcgctg caaagactcg gtcaagccgc tgcagccggt gaagagcagg
     6961 catgccgccg caatcacggc caaaatccgt tttttcatgc tctttcaccc ctcttcccct
     7021 atttaaaacg gcgggcgccg ccatttccct acagaactcg gcgcccttta ttagtaacta
     7081 tatggcgcct ttgcggcgca aagaccgctg ccagcagccg tgctcggcgg gctttgaaat
     7141 tgcacggcac agggacggct tgtggtttag tatagcataa acggccgcgt tttttcgaca
     7201 aaaacgcggc cgttttcata tttaacagtt tggtaatttt ttgttttggc cccgcggccc
     7261 tgcccgccca cagccccggt gcggccggcg cgggccgtcc aaaaagggga tgtgcgcggg
     7321 cctaggtatg cgccgcagcc ccgggctttc agaccgcccg gcgcctcaca gcttttcgtc
     7381 cacccgcacc gctccgtagt tgcgcacact gatgacgtag cagctgccct tgccgaacgc
     7441 cgactccatg gcggcgatgt attgctcgag ctttgcgccc ggcacaaagg cctggatggt
     7501 gcccgcaaag ccgccgccct gcagcctcca cgccccctgc cccgccagaa tgtgctggct
     7561 gagcgccacg cccacggcca cgccctgctc acgcgggtgc ttgatgctgt aggcgttctg
     7621 attgtactca aaagaggagt gcccgccgtc gatgatcagt gccttgaacc gctcgatgtc
     7681 ccctgcgcgg accgcgtccc gcagctgacc tgcgcggcgg gtgtcggcaa agaaatggat
     7741 ggcgcgcagc accgcccggt cgcccgcttt ttcgcgcact tcggggatgt tggcgaagaa
     7801 ttccgcctcg tccacctccc gcagcacctc tttgccaaaa atggcggcca cctgctccat
     7861 ctcggcgcgg atcgcggcgt agtcgtcggt gaggtccgca tggctgccct tggtgtcggt
     7921 gatgcacagg gataagccgt attttgccag atcaaaggcg acttcctcca ccgccgggtg
     7981 ggcggggtcc ttgaaatcaa tggtgatggc gctgcccacg ctgctggccg tctggtccat
     8041 caggccgctg ggcttgccga aatagacgtt ctccgcgtac tggctgatct gtgcgacctc
     8101 caccggcgtg aagcggaagc tgttgaactc cccgttaaag accgccccga tgaccacctc
     8161 gaacgcggct gaggaggaca gcccgctgcc ccgcagcacg tcgctggtgg tataggcgtc
     8221 aaagccgccc acctcgccgc cgagctgccg gatgcgcgcc gccacaccgc ggatcagggc
     8281 gctgctgcgc ccgaactccc gctcgttcac cttgtcgtcg ctcacgtcca ccacatccgc
     8341 gtcgcgaaag ccgtgggact tgacccggac gatggtggaa tcgttgcggg agaccacggc
     8401 gatgatgtcc aggttcaccg ccgccgccat gaccacgccg ttgttgtggt cggtgtggtt
     8461 gccgccgatc tcggtgcggc ccggcgccga gtagagctcc a
//
LOCUS       DBMDYA010000028         8033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2374_length_8033_cov_1.521306, whole genome shotgun sequence.
ACCESSION   DBMDYA010000028 DBMDYA010000000
VERSION     DBMDYA010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_8033_cov_1.521306"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ACZPFK_01320"
     CDS             complement(<1..268)
                     /locus_tag="ACZPFK_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404979.1"
                     /translation="MIAQLARRMLELSFTVGLLILLFALLRPLAAKWYTARWRCAVWL
                     LFALRLLIPWQFSLPERAAPVTVVVPDAVVWRGAADAAAPQATGG"
     gene            complement(265..657)
                     /locus_tag="ACZPFK_01325"
     CDS             complement(265..657)
                     /locus_tag="ACZPFK_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1404980.1"
                     /translation="MKRSLKRLPEAELELMLCLWEAEGPVPRSYFDARLRDSRGWADS
                     TVLSLLSRLEEKGFLKVEKRGNRNIYSPLVSRQAYLDLENRSFLARLHHDSLCDMVAG
                     MAQAKALTRQDMDDLQKLIDTLRRGEEK"
     gene            complement(663..944)
                     /locus_tag="ACZPFK_01330"
     CDS             complement(663..944)
                     /locus_tag="ACZPFK_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404981.1"
                     /translation="MKKVPCKKGWQSLRIVNLFQCGKQNVGSAGTAGPKPMFSFCGAF
                     KSSFLFPLVVDAQKNGAAFVGNGERAEKRLTENSDKCRITAVNNYICRR"
     gene            complement(1187..1948)
                     /gene="udp"
                     /locus_tag="ACZPFK_01335"
     CDS             complement(1187..1948)
                     /gene="udp"
                     /locus_tag="ACZPFK_01335"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1404982.1"
                     /translation="MHQHEKLYHIGLCKADLEGADYAILPGDPGRVEKIAALMENAKE
                     IGRNREYTSWLAETKGVKMLVMSTGMGGPSTAIGVEELHMLGVSHLIRVGTCGGMQLQ
                     VQAGDLVVVNGAIRQEGTSKEYLPVEFPAIADIDVTVALRQAARELGLPNHTGVVQCK
                     DSFYGQHDPARMPVSYELENKWQAWIKGGALGSEMETAALYTVSQVLGVHAGAVMLCV
                     WNQEREKAGLDQKETHDTTGAIRAAIRAVEILAGV"
     gene            2423..2695
                     /locus_tag="ACZPFK_01340"
     CDS             2423..2695
                     /locus_tag="ACZPFK_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1404983.1"
                     /translation="MKGNPEERAVALGEYILETGQTVRAAARVFGISKSTVHKDVTSR
                     LRRVNHSLYDEVQKVLTQNKQERHIRGGMATRHKYHPEEPVPAPEA"
     gene            2844..4268
                     /locus_tag="ACZPFK_01345"
     CDS             2844..4268
                     /locus_tag="ACZPFK_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1404984.1"
                     /translation="MSELERVGRLVDGAAAKAVELNKKIWSYAELNFREDKSAAALAD
                     ALEAEGFAVERGVAGVRTAFVASFGGGKPVIALLGEYDALSSLSQKAACPRREPVAEG
                     APGHGCGHSLLGAGAFAAAVAVKRYIEETGLAMTVRFYGCPAEEDGGGKVYMAREGMF
                     DDVDAALTWHPDAVNATKGVGSLAVMGVLYSFKGRTAHAAAAPQAGRSALDAAELMNM
                     GCQFLREHMSPDCRLHYAFRDVGGAAPNVVQASASLHYYIRAGRVDEMFKLFERVNKV
                     AAGAAMMTETEMEYRVIDGFSDYVPNRTLSELMARCMDEVGAPAFDDADRALAARFAA
                     TLSEDERRNNLESACQDNGLAYRRFDGKVLDDTVIPFCYQPGRPSPGSTDVGDVSYCA
                     PTAQFQMATMALGTGGHTWQMTAQSDSSIGEKGMLAAAKVLALTAVRAAADPQLLAAA
                     RAEWRQSCPDGYRCPMPAENRPEF"
     gene            4282..4698
                     /locus_tag="ACZPFK_01350"
     CDS             4282..4698
                     /locus_tag="ACZPFK_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1404985.1"
                     /translation="MWAVYAAGSALFASLTAILAKIGIDGVNSNLATAVRTIFVLAMA
                     WVTVGVTGNLSGVREITWRGWLFLALSGLATGASWLCYYRALQLGQVAKVVTVDKFSL
                     VLTILLGALLLGEKLNVKTVLGALLVTVGTLVIAFA"
     gene            4912..>8033
                     /locus_tag="ACZPFK_01355"
     CDS             4912..>8033
                     /locus_tag="ACZPFK_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1404986.1"
                     /translation="MSIGYLQIFSRSATDGLPVPGVTARVRPSAGTGDARNVTTDESG
                     YADPISLTAPDKSYSLDESNNSVLPYATYDITVDHEGYYPIVMRGVQVFDGENSLAEL
                     NMIPVEDNAQFRAEPEIFDIPEHALFSGDGGTGAEPAVQCERILAEVVIPNKIVVHLG
                     RPAVSARNVTVPFRDYIKNVASSEIYPTWPVEALKANIYAQISLALNRIFTEWYRSKG
                     KGYDFDITNSTSYDQYYVHGRNIFESISKVVDEVFNTYIRKQGTIEPYYAEYCDGKSV
                     TCPGMKQWGTKSLADQGYSAIRILRYYYGNSVELVTTNNIKDVQESYPGTPLRVGSTG
                     DAVRVIQRQLNRIVKNYPFMGAIPNVNGVFGADTESTVKKFQKQFSLTADGVVGRATW
                     YKISYIYASVKKLAELTSEGEEPSGQPSSGTYPGAALREGSTGASVRQIQFWLSTVSG
                     YVASVPDVAVDGVFGPSTTASVKAYQKHVGLVDDGVVGRATWDRLYADYQSIEGDISD
                     SDINFPGKYPGTLQQVGSRGNSVRQLQFWLSVLSDFYSTIPAVTPDGIFGSATEAAVR
                     AFQARFSLTADGIVGPATWNKLYEVYTSQINGILDENETPGVYPGSPLRVGSTGIKVK
                     EAQFYLYLLSAYYTSIPRIAYDGIFGTATRDAVVAFQRIANLTADGVIGPATWQALYS
                     TYQKLRTNDGPVIAYQFEPYPGHPVAENTEGQAVRFIQFMLALGELYYPALREVEITG
                     FYDEETRLAVEAFQDEFNIPVTGVVDEETWNAMLEMLFELLSSSGGIGNTPTGDYPGY
                     VLTVGSAGPAVRELQSYMNQIAERYCAAGFVRQDGYFDEATREAVMAFQEGFGLPVTG
                     FVDRETWDVIYNYYLTLTGRATAKTKIWVYDEYENRMMVYYRAESESMPYSYGTTLRV
                     REFRGSSKSPTLWTTVRAMESWNQTRRSYGSGIPVGYAFKRIWEGGHGTASQHYAGVS
                     FDVGQNVSYSQRVASRNAAVATGAWGYVEPISMTPTWVHFDRRYGTPACSGTTAGYPT
                     VRRGSRSTYVL"
BASE COUNT         1404 a         2735 c         2526 g         1368 t
ORIGIN      
        1 cgccgccggt cgcctgcggt gccgcagcgt cggccgcgcc ccgccagaca acggcgtccg
       61 gcaccaccac cgtcaccggg gccgcccgct cgggcaggga gaactgccag gggatcaaga
      121 gccgcagggc aaacagcagc cagaccgcgc agcgccagcg ggcggtatac catttcgccg
      181 ccagcgggcg cagcagcgcg aacagcagga tcaagagccc caccgtgaaa gagagctcca
      241 gcatgcggcg ggccagttgt gcgatcattt ttcttccccc ctccgcagtg tgtcgatgag
      301 cttttgcaga tcgtccatgt cctggcgggt cagcgccttg gcctgggcca tccccgccac
      361 catgtcgcag agagagtcgt ggtgcaggcg ggccagaaag ctgcggttct ccaggtcaag
      421 atacgcctgt cttgagacca ggggggaata gatgttgcgg ttgccccgct tttccacctt
      481 taaaaacccc ttttcctcca ggcgggagag caggctgagc accgtgctgt ccgcccagcc
      541 gcggctgtcc cgcaggcggg cgtcaaaata gctgcgcggc acggggccct ccgcctccca
      601 aaggcagagc atcagctcca gctcagcctc gggcaggcgc ttcaagctgc gtttcatccc
      661 catcatctcc gacaaatgta gttgtttaca gctgtaattc tacatttgtc ggagttttct
      721 gtcaatcttt tttccgctct ttccccattc ccgacaaaag cagccccatt tttttgtgca
      781 tccacaacaa gagggaaaag aaagctgctt ttgaacgcgc cgcaaaaaga aaacattggt
      841 tttggcccgg cggtcccggc gcttcccaca ttttgcttcc cacattgaaa aaggttgaca
      901 atccgcaggg attgccaacc ttttttacag ggtactttct tcaaaatcac ctcgtcaaaa
      961 ccgcccggcg ccgcaggggg aaagctggga tgccaagccg agagcatcca cacagaacgc
     1021 cacgcgcggg cggcccacag ggcgaaaccg caaaccttag tggccgtgag ggaaacccga
     1081 cgacgctccg cgcgcaaatt tccctctcgc ggacaatatt tttttgcaaa agcgctccct
     1141 tggcagcccc ttttgtcctt tcgcccgccc accggcagcg tccccctcac accccggcca
     1201 ggatctccac cgcacggatg gcggcccgga tagcgccggt ggtgtcatgg gtctcctttt
     1261 ggtccaaacc ggccttttcg cgctcctggt tccagacgca gagcatcacc gcgcccgcgt
     1321 gcacgcccag cacctggctg accgtgtaaa gggccgcggt ctccatctcg ctgcccagcg
     1381 cgccgccctt gatccaggcc tgccacttgt tttccagctc gtagctcacc ggcatgcggg
     1441 cggggtcgtg ctggccgtaa aagctgtcct tgcactgcac cacgccggtg tggttcggca
     1501 ggcccagctc ccgcgcggcc tgccgcaggg cgacggtgac gtcaatgtcc gcaatggcgg
     1561 ggaactcgac gggcagatat tccttgctgg tgccctcctg ccggatggcg ccgttgacca
     1621 ccaccaggtc gcccgcctgc acctgcagct gcatgccgcc gcaggtgccc acccggatca
     1681 gatgggacac gcccagcatg tgcagttcct ccaccccgat ggcggtggac ggcccgccca
     1741 tgccggtgct catcaccagc atcttgacgc ccttggtctc ggccagccag ctggtatact
     1801 cgcggttgcg gccgatctcc tttgcgttct ccatcagggc ggcgatcttc tccacccgcc
     1861 cggggtcccc cggtaaaatg gcgtagtccg cgccctccag gtccgccttg caaaggccga
     1921 tatggtacag tttttcatgt tgatgcatga tgtcacccgc tttccgtgcc aaaacggcac
     1981 aaaatgtttt ccatcgcgac ggcgccgccg ctgtgtcctc cttttcccgc ccgtcggcgg
     2041 cacggtgggc cgcgtctgcg cgggaggggg ccgcgctgcg ggcggccccg caggggccgg
     2101 tccaccgcgc cgccgaaacc gcctgcgccg ccgtcttcct gtttttcatt ataccacaga
     2161 tcggcccgat accggtatcc ccaaaagctg ttctcccctt tttctccctt tcccgtcccg
     2221 ttttcctccg cctgtgctcc ctgcgcgccg cggagcgcgc ggaaaagcgc ccctgcgggc
     2281 cctcgtccgc cgcctcgccg cctccatcgg ccgggcgggc cctttcccgc ataacccctg
     2341 tccccctttg cataagatga ggtaacgggc gcgtccgggc acggctgcgc cctatcctga
     2401 tatggtaaag gtggaatcac gtatgaaagg caaccccgaa gagcgggccg tcgcgctggg
     2461 cgaatacatc ctggagaccg ggcagaccgt ccgcgccgcg gccagggttt tcggcatctc
     2521 caaatcgacc gtacataagg atgtgaccag ccgcctgcgg cgggtcaacc acagccttta
     2581 cgacgaggtg cagaaagtgt tgacccagaa caagcaggag cggcacatcc ggggcggcat
     2641 ggccacccgc cacaaatatc acccggaaga gccggtcccc gcgccggagg cgtgattttt
     2701 tcttgaaccc cgcccggttc tgtactatac ttttggcaga caggggcggt ccgcagcggc
     2761 cgaggcgcgg gcggggagca tatgctctcc ccccgcgggc cgtgcggcgg cccccagaaa
     2821 ccgaagcagg gggaaacagg gctatgagcg aactggaacg ggtcggccgt ctggtcgacg
     2881 gcgcggcggc aaaggctgtg gagctgaaca aaaagatctg gagctatgcg gagctgaatt
     2941 tccgcgagga caagagcgcc gccgctttgg cggacgcgct ggaggccgag ggcttcgcgg
     3001 tggagcgggg cgtggccggc gtgcgcaccg cattcgtggc cagctttggc ggcggcaagc
     3061 cggtgatcgc gctgctgggt gaatacgacg cgctgtccag cctgtcccaa aaggcggctt
     3121 gcccgcgccg cgagcccgtc gccgagggcg cgccgggaca cggctgcggc cacagtctgc
     3181 tgggggcggg cgcctttgcc gccgcggtgg cggtgaagcg ctacatcgaa gagacgggtc
     3241 tcgccatgac ggtgcgcttt tacggctgcc cggccgagga ggacggcggc ggcaaggtgt
     3301 acatggcccg cgagggcatg ttcgacgacg tggacgccgc gctgacctgg cacccggacg
     3361 cggtgaacgc caccaagggg gtgggcagcc tggccgtgat gggggtgctc tactcgttca
     3421 aggggcgcac cgcccatgcc gccgccgcgc cgcaggcggg gcgcagcgcg ctggacgcgg
     3481 ccgagctgat gaacatgggc tgccagttcc tgcgggagca tatgtccccc gactgccgcc
     3541 tgcactacgc gtttcgcgac gtcggcggcg ctgcgcccaa cgtggtgcag gccagcgcca
     3601 gcctgcacta ttatatccgc gccggccggg tggacgagat gttcaagctc ttcgagcggg
     3661 tgaacaaggt cgcggcgggc gccgccatga tgaccgagac cgaaatggag taccgggtca
     3721 tcgacggctt ttccgactat gtgccgaacc ggacgctcag cgagctgatg gcgcgctgca
     3781 tggacgaggt gggcgccccg gcgtttgacg atgccgaccg ggcgctggcc gcccggtttg
     3841 ccgccactct cagcgaggac gagcggcgca acaatctgga atccgcctgc caggacaacg
     3901 gcctggccta ccgccgcttt gacggcaagg tgctggacga cacggtgatc ccgttctgct
     3961 accagccggg gcgtccctcc cccggcagca ccgacgtggg cgacgtgagc tattgcgccc
     4021 ccaccgccca gttccagatg gccacgatgg cgctgggcac cggcggccac acctggcaga
     4081 tgaccgccca gtcggacagc tccatcggcg aaaaagggat gctggcggcg gccaaggtgc
     4141 tggccctcac cgccgtgcgg gccgcagccg acccgcagct gctggccgcg gcccgggccg
     4201 agtggcggca gagctgcccg gacggctacc gctgccccat gccggctgaa aatcggccgg
     4261 aattttgagg gggcgcgcgg tatgtgggca gtttatgccg ccggttcggc gctgttcgcc
     4321 agcctcaccg ccattttggc caagatcggc atcgacggcg tcaactcgaa cctcgccacc
     4381 gccgtgcgca ccatctttgt gctggcgatg gcctgggtga cggtgggggt cacgggcaac
     4441 ctatcgggcg tgcgcgagat cacctggcgc gggtggctgt ttctggcgct ttccggcctt
     4501 gccaccgggg cgagctggct gtgctattac agggcgctgc agctcggcca ggtggccaaa
     4561 gtggtcaccg tcgacaagtt cagcctggta ttgaccatcc tgctcggcgc gctgctgctg
     4621 ggcgaaaagc tgaatgtgaa aacggtgctg ggcgcgctgc tggtcaccgt cggcacactg
     4681 gtgatcgcgt ttgcctgacc ttgggacagc cgtttgggca gccgcacaga aaaagagctt
     4741 ccttttctgt gcggctgttt ttttgtcccg caaagaggcg gccttacccc cgcccgcggc
     4801 ccgtcccttt cccttttgct gtggcgcgtc ccgccgcccc cgcataccct gtggtaacgg
     4861 cccagggcgc gctttgcgcc cctcgccgaa actttcagga ggaatgcctt catgagcatt
     4921 ggttatcttc agatattctc ccgctcggcg accgacggcc tccctgtgcc cggcgtgacc
     4981 gcccgcgtgc ggccctcggc cggcaccggg gacgcgcgca acgtcaccac cgacgagtcg
     5041 ggctatgccg accccatcag tctcaccgcg cccgacaaga gctactcgct ggacgagagc
     5101 aacaactcgg tgctgcccta cgccacctac gacatcacgg tggaccacga gggctactac
     5161 cccatcgtca tgcggggcgt gcaggtgttc gacggtgaaa actccctcgc cgagctcaat
     5221 atgatcccgg tggaggacaa cgcccagttc cgggccgagc cggagatctt cgacatcccc
     5281 gagcacgcgc tcttttccgg cgacgggggc accggcgccg agcccgccgt gcagtgcgag
     5341 cgcatcctgg ccgaggtggt catccccaac aagatcgtgg tgcacctggg tcggccggcg
     5401 gtcagcgccc gcaacgtgac ggtgcctttc cgcgattaca tcaaaaacgt cgccagcagc
     5461 gagatctatc ccacctggcc ggtggaagcg ctgaaagcca atatctacgc ccagatcagc
     5521 ctggccctga accgcatctt taccgagtgg taccgcagca agggcaaggg ctacgacttc
     5581 gacatcacca actccaccag ctatgaccag tactacgtgc atgggcgcaa tatctttgag
     5641 tccatcagca aggtggtgga cgaggtcttc aacacctaca tccgcaagca gggcacgatc
     5701 gagccgtact acgccgagta ctgcgacggc aaatcggtca cctgccccgg catgaagcag
     5761 tggggcacca aaagcctggc cgaccagggc tacagcgcca tccgcatcct gcgctattac
     5821 tacggcaaca gcgtcgagct ggtcaccacc aacaacatca aggacgtgca agagagctat
     5881 cccggcaccc cgctgcgggt gggcagcacc ggcgacgctg tgcgcgtcat ccagcgccag
     5941 ctcaaccgca tcgtaaagaa ctatcccttt atgggcgcca tccccaacgt gaacggggtg
     6001 ttcggcgccg acaccgagag caccgtgaaa aagtttcaaa agcagttcag cctcaccgcc
     6061 gacggcgtgg tgggccgggc cacctggtac aagatcagct atatctatgc ctcggtgaaa
     6121 aagctggcgg agctgaccag cgagggcgaa gagcccagcg gacagcccag cagcggcacc
     6181 taccccggcg ccgccctgcg cgagggcagt accggcgcct cggtgcgcca gattcagttc
     6241 tggctgtcca ccgtgtcggg ctatgtggcc tcggtgcccg acgtggcggt ggacggcgtc
     6301 ttcggcccct ccaccaccgc ctcggtgaaa gcgtatcaaa agcatgtggg tctggtggat
     6361 gacggcgtgg taggacgcgc cacctgggac cgtctctacg ccgactacca gagcatcgag
     6421 ggggacatct ccgactcgga tatcaacttc cccggcaaat accccggtac cctgcagcag
     6481 gtgggcagcc gcggcaacag cgtgcggcag cttcagttct ggctgtcggt actcagcgac
     6541 ttctactcca ccatcccggc ggtgacgccg gacggcatct tcggcagcgc aaccgaggcc
     6601 gcggtgcgcg cctttcaggc ccggttttca ctgaccgcgg acggcatcgt cggccccgcc
     6661 acctggaaca agctgtacga ggtctacacc agccagatca acggcatcct ggacgagaac
     6721 gagacgccgg gcgtctaccc gggcagcccg ctgcgggtgg gcagcaccgg catcaaggtc
     6781 aaggaggcgc agttctacct ctacctgctc tccgcctact acaccagcat cccccgcatc
     6841 gcctacgacg gcatcttcgg caccgccacc cgcgacgcgg tggtcgcttt tcagcgcatc
     6901 gccaacctga ccgcggacgg cgtcatcggc cccgccacct ggcaggccct gtacagcacc
     6961 taccagaagc tgcgcaccaa cgacggcccg gtgatcgcct accagttcga gccctatccc
     7021 ggccacccgg tggcggagaa caccgagggc caggcggtgc ggttcatcca gttcatgctg
     7081 gccctgggcg agctgtatta cccggccctg cgcgaggtgg agatcacggg cttttacgat
     7141 gaggagaccc ggctggcggt ggaggctttt caggacgaat tcaacatccc cgtcaccggc
     7201 gtggtggacg aggagacctg gaacgccatg ctggagatgc tgtttgagct gctctcctcc
     7261 tccggcggca tcggcaacac ccccaccggc gattatccgg gctatgtgct caccgtgggc
     7321 agcgccggcc cggccgtcag ggagctgcag tcctatatga atcagatcgc cgagcgctac
     7381 tgcgcggcgg gctttgtgcg gcaggacggc tatttcgacg aggccacccg cgaggcggtg
     7441 atggcttttc aggagggctt cgggcttccg gtcaccggct ttgtggaccg ggagacctgg
     7501 gacgtgatct acaactacta cctcaccctc accggccgcg ccaccgccaa gaccaagatc
     7561 tgggtctacg acgagtacga gaaccgcatg atggtctact accgggccga gagcgaatcc
     7621 atgccctact cctacggcac cacgctgcgg gtgcgggagt tccgcggcag cagcaagagc
     7681 ccgaccctct ggaccaccgt gcgcgccatg gagagctgga accagacccg ccgcagctac
     7741 ggcagcggca tcccggtggg ctatgcgttc aagcgcatct gggaaggcgg ccacggaacg
     7801 gccagccagc actacgcggg cgtgagcttc gacgtggggc agaacgtttc gtattcccag
     7861 cgcgtggcca gccgcaacgc ggctgtggca accggcgcct ggggctatgt ggagcccatc
     7921 tccatgacgc cgacctgggt gcatttcgac cgccgctacg gtactcccgc gtgcagcggc
     7981 accacggcgg gctaccccac cgtgcggcgc ggcagccgca gcacctatgt gct
//
LOCUS       DBMDYA010000029         7960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2400_length_7960_cov_1.581117, whole genome shotgun sequence.
ACCESSION   DBMDYA010000029 DBMDYA010000000
VERSION     DBMDYA010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2400_length_7960_cov_1.581117"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="ACZPFK_01360"
     CDS             complement(<1..699)
                     /locus_tag="ACZPFK_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1404987.1"
                     /translation="MTDRQEKILGGLLAAAIGDSMGAVTEHASRRKLEKDYGWVDTFI
                     EPHESLKAKGHRIGLVTDDFSVAYYSALPLIEHRQKVTRELAIEGLLRWNEQPLYAQC
                     AGPTTITTINRLKGIPSPPSRGELLLCDNSRVTNGGGMKSGMMGLFNPGDPDAAVDDT
                     ITLCGLTHDNTIALSAACAIAAATAKAFCPGASYLDLIEAGIYGAEQGYARSLADFRP
                     VTASSISKRIALAAE"
     gene            complement(719..1645)
                     /locus_tag="ACZPFK_01365"
     CDS             complement(719..1645)
                     /locus_tag="ACZPFK_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404988.1"
                     /translation="MLKSILELAFTASTFAATFRLACPILFGAMGGCFNHTSGCVNIA
                     YEAVMLYSCFFSILGSYLTGSPWVGLLCGIGVGLLISVIFGLMAFVFKANTLIVGIAL
                     NSSAWAVTTLLMVIIFQVRGSVYNERIVSFSPIHFKFLEKIPLLDSVFNNNIILVYLS
                     FLMVIAAFVVMYKTPFGLRIRGIGQNAQAAYTAGIDVEKYRWISLLLMGTFTAVSGTF
                     LTLSGISMFSENMTAGRGFLCMCSIAIGKGNPLKILLVALLFAYSQALVLVLGTLSIP
                     TQIISMIPYVAVILVLFFNGLKSFKGTADIEV"
     gene            complement(1638..2774)
                     /locus_tag="ACZPFK_01370"
     CDS             complement(1638..2774)
                     /locus_tag="ACZPFK_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1404989.1"
                     /translation="MKNKLFSFRVKKLGKDLAQNDRLVINALKYGVSIILFLLCGSLL
                     ILLQGQSPLAALEAIFQGAFGNKMAIGNTLRWTAPCIMTGAAAIVAFKAGISNLGIAG
                     QVTIGGFLAGLIGAFYELPPLAHITLIIVVAGVAGLLWSVVPAILRLFFNINEFITSL
                     MLNYVANFLTQYLTQQVLAGGLDSWGYVNMEATPTVLSSAVLPDLIHGTSATLAVPIA
                     LIVAVAIAFLYKYTIQGYELKQVGENLKFAKVGGVKVVKQFLIIFLLSGFISGMCGGI
                     EVIGTYKKFNTGFADTMGWDGISIARIAELNPVALIFVSFIWAALKAGAMQMERVISV
                     NRLTVQIVQYLFVLFVSVDYEGIYLRAKERRAKKQYRKEMEKVA"
     gene            complement(2764..>3421)
                     /locus_tag="ACZPFK_01375"
     CDS             complement(2764..>3421)
                     /locus_tag="ACZPFK_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1404990.1"
                     /translation="AGVAGSGQQQLVEALFGLRAPETGSAVYFEGKDITRATPRQHRC
                     MGIGYVPQDRLGAGANGQSSIWETAIMGYHVAHGFKSRFLLNFKQIEEFTNRIVDDFA
                     VKRQKNSDKIRSLSGGNIQKVVVGREFLQDNKLLIIEDPTRGIDVGAIEFIWKKIIDL
                     AAGGVSVLLISHELNEVMQLADTIKVVYEGKLYDGGAHGDLTEEEIGLIMMGGKSDEK
                     "
     assembly_gap    3422..3431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3432..4294)
                     /locus_tag="ACZPFK_01380"
     CDS             complement(<3432..4294)
                     /locus_tag="ACZPFK_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1404991.1"
                     /translation="MEKPDYILQMKEIRKTFGPLVANDNVNLNIERGTVHAIVGENGA
                     GKSTLMNILTDIYKADSGDILLNGKKVVFKNSLDAARHGIGMIYQEFMLYPELSVYDN
                     LILGFEEKKLGVFIDKKACKKKIGQICRDYSFDLPMKALVKDLPVAVLQQVEIVKALY
                     RSADIIIMDEPTSVLTPQGIQDLFRAMRNLVAAGKTIIFISHKLKEVFEIADRITVMK
                     DGRISGEVLPSEVTENQLASMMVGRDVILQANKLDVAFGEPVLSVKNLKVKDKDGVLR
                     VKGVDLDVRRG"
     gene            complement(4372..5445)
                     /locus_tag="ACZPFK_01385"
     CDS             complement(4372..5445)
                     /locus_tag="ACZPFK_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1404992.1"
                     /translation="MKKLFSLLLVLALGMGLAACGSSDSSGSAAGSVASGGAGSSAAV
                     SDKEKKTCLISDSSMAADFDQVIWRGFTMLEANGWEVKCIEALDKAEYEEDIYAMAAA
                     GYKLIYIKGDAIASVLIDIADEFHAMYPDVYFILVDSYLEHDLDFATAVPVDPYESSF
                     MGGYVAALMSKTKEIGWIGHMDTVNLARFRNGFIAGAQYAVPECKVAVGFTGDFYDPI
                     KGQEATYAMHENNPNVDIIAHAAYISGNGVLSACGELGIPCIGCDAWQGDKGDTVFWS
                     TLKSVDLMVFNTANRWLNNEEGAFGPKMSFNIAAGSVPYDTRDLSALPQDVQDKVQEL
                     MEGIKSGSIDVFYGDYAEYKLDY"
     gene            5832..7073
                     /locus_tag="ACZPFK_01390"
     CDS             5832..7073
                     /locus_tag="ACZPFK_01390"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HIR1404993.1"
                     /translation="MRTDLKRIQRDIEALAAFNSTPGAGLTRFSFSEEHKRAQDYIVA
                     EMEKAGLQVRVDACGTVIGRLEGEDPDAPVIVTGSHYDSVRNGGNFDGPAGVITGLET
                     ARVFHENKIRPRRPVEFIAMVEEEGARFGGGLFASRAMTGRLTAEELATFADADGVTT
                     GEAMRAFGLDPAAYKQAVRRKGEIKNFIELHIEQGPVLEAGNIQVGIVETIVGIKEVE
                     VTVTGRPDHAGTTPMEMRADAFLVAAKAAVAANEAALRVGEGTVATVGKVQVQPGSFN
                     IVAGKVTFYIDIRSKKRACVDAVLDAVKEKLDELTAGNEGLSYELRMMLATDPVDTDP
                     HVCAALEQSARELGLSSKRMLSGAGHDAMIMADIAEIGLVFVPSRGGRSHCPEEWTDY
                     DLLQNGIETVCATVEKLACEG"
BASE COUNT         1609 a         2251 c         2513 g         1577 t
ORIGIN      
        1 ctcagcggcc agggcgatcc gcttggaaat gctgctggcg gtcacggggc ggaaatccgc
       61 cagcgagcgg gcatagccct gttcggcgcc gtagatgccg gcctcgatca ggtccagata
      121 agacgcgccg gggcaaaagg cctttgcggt ggccgccgcg atcgcgcagg cggcggacag
      181 cgcgatggtg ttgtcgtggg tgagcccgca aagggtgatg gtgtcgtcga cggcggcgtc
      241 cgggtcgccg gggttgaaca gccccatcat gccggatttc atgccgccgc cgttggtcac
      301 ccggctgttg tcgcacaaaa gaagctcgcc cctggagggg ggagagggaa tccctttcag
      361 ccggttgatc gtggtgatgg tggtgggccc ggcgcactgc gcgtagagcg gctgctcgtt
      421 ccagcggagc agcccctcaa tcgccagctc ccgggtgacc ttctgccggt gctcgatcag
      481 cggaagcgcc gagtagtacg ccaccgaaaa atcgtctgtc accaaaccga tcctgtgccc
      541 cttggctttt aagctttcgt ggggctcgat gaaagtgtcg acccagccgt agtccttttc
      601 cagctttcgc cgggaggcgt gctcggtgac agcgcccatc gagtcgccga tcgccgcggc
      661 gagcagcccc cctaggattt tttcctgcct gtcagtcatt ctgatcctcc ttgtctggct
      721 atacttcgat gtcggcggtc cccttgaagc tcttgaggcc attgaagaag agcaccagaa
      781 tgaccgcaac ataggggatc atggagatga tttgagtggg gatgctgagg gtgccgagga
      841 ccagcaccag agcctgcgaa taggcgaaca gcagcgccac cagcaggatc ttgagcgggt
      901 tgcccttgcc gatggcgatg gagcacatgc acagaaagcc ccgcccggcc gtcatgttct
      961 cagaaaacat ggagatgccc gaaagggtca gaaaggtccc cgagacggcg gtgaacgtgc
     1021 ccatcagcag cagcgagatc cagcggtatt tttccacatc gatgcccgca gtgtaagccg
     1081 cctgcgcgtt ttgcccgatg ccgcggatgc gcagcccgaa aggcgttttg tacatcacga
     1141 caaaggccgc aatcaccatc agaaaggaga gatagacgag gatgatgttg ttgttgaaga
     1201 cagagtccag caggggaatc ttctccaaaa atttgaagtg gatggggctg aagctgacga
     1261 tccgctcgtt gtagaccgaa ccgcggacct ggaagatgat caccatcaag agagtggtca
     1321 cggcccaggc gctggagttg agggcgatgc cgacaatgag ggtgttggcc ttgaagacaa
     1381 aggccatcag cccaaagatg accgagatga gcaggccgac gccgatgccg cagagcagac
     1441 ccacccaggg gctgccggtc agatagctgc cgaggataga gaaaaagcag gagtagagca
     1501 tgactgcctc ataggcgatg ttcacgcatc cggaggtgtg gttgaaacag cctcccatag
     1561 ccccgaagag gatcgggcag gccaggcgaa acgtggcggc aaaggtggag gccgtaaacg
     1621 cgagctccaa aatggattta agcaactttc tccatctcct ttctgtactg cttctttgcc
     1681 cggcgctctt tcgcgcgcag gtagatgccc tcgtaatcga cagagacgaa gaggacgaag
     1741 aggtactgga cgatctggac ggtcaagcgg ttgacggaga tcacccgctc catctgcatg
     1801 gcgccggctt tgagggcggc ccagatgaaa gagacgaaga tgagggcgac cgggttcagc
     1861 tcggcgatgc gggcgatgct gatgccgtcc cagcccatgg tgtccgcaaa gccggtgttg
     1921 aattttttgt aggtgccgat gacctcgatg ccgccgcaca tgccggagat gaaacccgac
     1981 agcaaaaaga tgatcagaaa ttgcttgacc accttcaccc cgccgacctt ggcgaatttc
     2041 aggttctcgc ccacctgttt gagctcgtat ccctgaatgg tgtatttgta caggaacgcg
     2101 atggcgaccg ccacgatcag ggcgatgggg acggcgaggg tggccgaggt gccgtggatg
     2161 agatcgggca gcaccgcgga ggagaggacc gtgggcgtgg cctccatgtt cacatagccc
     2221 caggagtcca gcccgcccgc cagcacctgc tgggtgagat attgggtcag aaaattggcc
     2281 acatagttca gcatcaggga ggtgatgaac tcgttgatgt tgaaaaacag gcgcaggatg
     2341 gccggcacca ccgaccacag caggccggcg acgcccgcga ccacgatgat cagggtgatg
     2401 tgggccagcg gcggcagctc gtaaaacgcg ccgatcaggc cggcgagaaa gccgccgatg
     2461 gtgacctggc cggcgatgcc caggttgctg atgccggcct tgaacgcgac gattgcggcg
     2521 gcgccggtca tgatgcaggg cgccgtccag cgcagggtgt tgccgatggc catcttgttg
     2581 ccgaaagcgc cctgaaaaat ggcctccagc gcggccaggg ggctttgccc ctgcagcagg
     2641 atcagcaggc tgccgcaaag caggaacagg atgatcgaga cgccgtattt cagcgcgttg
     2701 atgaccagcc ggtcgttttg ggccagatcc ttgcccagct ttttgacccg gaaagagaaa
     2761 agcttatttt tcatccgatt ttccccccat catgataagg ccgatctcct cttcggtgag
     2821 gtcgccgtgg gcgccgccgt cgtacagctt gccctcatac acgaccttga tggtatccgc
     2881 cagctgcatg acctcgttga gctcatggct gatgaggagg acggagaccc cgcccgcggc
     2941 caggtcgatg atctttttcc agatgaattc aatggcgccc acgtcgatgc cgcgggtggg
     3001 atcctcgatg atgagcagct tgttgtcctg caaaaactca cggccaacca cgactttttg
     3061 gatgttgccg cccgacagac tgcggatctt gtctgagttt ttctgacgtt tgacggcgaa
     3121 gtcgtccacg atgcggtttg taaactcctc gatctgcttg aagttcagca gaaaacggct
     3181 cttgaagcca tgggccacgt gatagcccat gatggcggtc tcccagatgc tggactgccc
     3241 gttcgctccc gccccgaggc ggtcctgggg gacatagccg atccccatgc agcggtgctg
     3301 cctgggggtg gcgcgggtga tgtccttgcc ctcaaaatag actgcgctgc ccgtctcagg
     3361 ggcgcgcagg ccgaaaagcg cctccaccag ctgctgctgg cccgagcccg ccacgccggc
     3421 annnnnnnnn ntccccgcgc cgcacgtcca ggtcgacgcc tttgacccgc agaacgccgt
     3481 ctttgtcctt gactttcagg ttctttaccg acagcaccgg ctcgccgaac gcgacgtcca
     3541 gcttgttcgc ctgcaggatg acgtcgcgcc ccaccatcat cgaggccagc tggttctccg
     3601 tcacctcgct gggcagcacc tcgccggaga tcctgccgtc cttcatcacc gtgatgcggt
     3661 cggcgatttc aaaaacctct ttgagcttgt gggagatgaa gatgatcgtt ttgccggcgg
     3721 ccaccaagtt gcgcatcgcg cggaacaggt cctggatgcc ctggggggtg agcacggagg
     3781 tgggctcgtc catgatgatg atgtcggcgc tccggtagag cgccttgacg atctccacct
     3841 gctgcaaaac agccacgggc aaatctttca ccagcgcttt catcggcagg tcaaaggaat
     3901 agtccctgca gatctgcccg atcttttttt tgcaggcctt tttgtcgatg aacaccccga
     3961 gctttttttc ctcaaacccc aaaatcaggt tgtcgtagac cgacagctcc gggtagagca
     4021 taaattcctg gtagatcatg ccgatgccgt ggcgggcggc gtcgagagaa tttttgaata
     4081 ccactttttt tccgttgaga aggatgtcgc cgctgtccgc cttgtagatg tcggtgagaa
     4141 tattcatgag cgtgctcttg cccgccccgt tttcgcccac gatggcgtgg acggtgcccc
     4201 gctcgatgtt gagattgacg ttgtcgttgg caacgagcgg cccaaaggtc ttgcggatct
     4261 ctttcatctg aagaatgtaa tcaggtttct ccatgaggcc ctcctgtctt gggtgataaa
     4321 aaatggggtg atgcaagcgc tcaccccatt ttttcggccc tgttttaaga atcagtaatc
     4381 gagcttgtat tcggcgtagt caccgtagaa tacatcgatc gaacccgatt tgatcccctc
     4441 catcagttcc tgcaccttgt cctggacatc ctgggggagc gcggagagat cgcgggtgtc
     4501 gtaagggacg gagccggcgg cgatgttaaa ggacatttta ggcccgaagg cgccctcctc
     4561 gttgttcagc cagcggttgg ccgtgttgaa caccatcagg tcgaccgatt tcagggtgct
     4621 ccagaaaacg gtgtcgccct tgtcgccctg ccatgcgtca cagccgatgc aggggatgcc
     4681 cagctcgccg caggcggaga gcacgccgtt gccggagatg taggcggcat gggcgatgat
     4741 gtccacattg gggttgtttt catgcatggc gtaggtggcc tcctggccct tgatggggtc
     4801 gtagaagtcg ccggtgaagc cgaccgccac cttgcactcg gggaccgcgt actgggcccc
     4861 ggcgatgaag ccgtttctga agcgggccag gttgacggtg tccatatggc cgatccagcc
     4921 gatctctttg gtctttgaca tcagcgccgc cacatagccg cccatgaacg aggactcgta
     4981 ggggtccacc ggcaccgcgg tggcaaagtc gagatcgtgc tcgagatagg agtcgaccag
     5041 gataaaatac acgtcgggat acatggcgtg gaactcgtcg gcgatgtcga tgagcacgga
     5101 ggcgatggcg tcgcccttga tgtagatcag cttgtaaccg gcggccgcca tggcgtagat
     5161 gtcttcctca tattccgcct tgtcgagggc ttcgatgcac tttacctccc agccgttggc
     5221 ctccagcatc gtgaagccgc gccagatgac ttggtcaaaa tccgcagcca tgctggagtc
     5281 ggagatgagg caggttttct tttccttgtc ggagacggcg gcggaggaac cggcgccgcc
     5341 gctggcgacg ctgccggcgg cgctgccgga actgtccgaa gagccgcagg cggccaggcc
     5401 catgccgaga gccaatacca gcagaagcga aaacaacttt ttcattattc tctccttttt
     5461 tcagttgggt ttgaccgtcc tgcaggaacg tgaatttggg gggctgcccc ttttcacctg
     5521 tagtataaat tgggaaaatg cataaagcaa gccggaaaaa cattttccat ttattgcaca
     5581 atgaacatag tttcgacttt attcaacata aagttcaact ggacatattt ttgaatcaaa
     5641 accatccgcc gccttttttg gcacattttc accgaatggc ggcgcggaaa aatcaatatg
     5701 cggacagact gcttttttac cggcagcggg agaaaagaca aaagacgatg ttttcctttc
     5761 tgcgcctctt ttttacggct aaaaaatccg ctataataaa cccatccgat caagagaggg
     5821 gaagcaaaat aatgaggact gacctgaaac gcatccagag ggatatcgag gcgctcgccg
     5881 ctttcaattc cacccccggc gcggggctga cccggttttc gttcagcgag gagcacaaaa
     5941 gggcgcagga ttacatcgtc gccgagatgg aaaaggccgg gctgcaggtg cgcgtggacg
     6001 cctgcggcac cgtcatcggc cggctggagg gcgaagaccc cgacgccccg gtgatcgtga
     6061 ccggcagcca ttacgactcg gtgcgcaacg ggggcaactt cgatggcccg gccggcgtca
     6121 tcaccgggct ggagacggcg cgggtctttc atgagaacaa gatccggccg cgccgaccgg
     6181 tggagtttat cgccatggtg gaggaggagg gcgcccgctt cggcggcggg ctgttcgcca
     6241 gccgggccat gaccggccgc ctcaccgccg aggagctggc gacctttgcc gacgccgacg
     6301 gcgtcaccac cggcgaggcg atgcgggctt tcggccttga ccccgcagcc tacaaacagg
     6361 cggtgcgccg caagggggag atcaaaaatt ttatcgagct gcacatcgag caggggcccg
     6421 tcctggaggc cgggaacatc caggtgggca ttgtggagac catcgtgggc atcaaggagg
     6481 tggaggtgac cgtcaccggc cgccccgacc acgcgggcac cacgccgatg gagatgcggg
     6541 cggacgcatt tctggtggcg gcgaaagccg ccgttgcggc caatgaggcg gcgctgcgcg
     6601 tgggcgaggg caccgtggcc acggtgggca aggtacaggt tcagccgggc tcgttcaata
     6661 tcgtggcggg caaggtgacc ttttatatcg atatccgcag caaaaagcgg gcctgtgtgg
     6721 acgcggtgct ggacgccgtc aaggagaagc tggacgagct gaccgcgggg aacgaggggc
     6781 tctcttatga gctgcgcatg atgctggcga ccgacccggt ggacacggac ccccatgtgt
     6841 gcgcggcgct ggagcagagc gcccgggagc tggggctctc cagcaagcgg atgctctccg
     6901 gcgcggggca cgacgcgatg atcatggccg acatcgccga gatcggcctg gtctttgtgc
     6961 ccagccgggg cggccgcagc cactgcccgg aggagtggac cgactatgat ctgctgcaga
     7021 acggtatcga gacggtctgc gccacggtgg aaaagctggc ctgcgagggc tgagccgcaa
     7081 agcggcggaa gcgcgggaga ggacattaaa aatggcctca gactattttg ggcggccata
     7141 tggtccatcg cagggctaca gccatgagga taaaatggcc ctattcggcg cgttggggca
     7201 aaaaatattg gttttgaacc ggcggctttg atgccctgta tagataaaaa aggttggcaa
     7261 tcaacagaga ttgccaacct tttttgtatt gtgttttgga taaagccgtc gggtcgaagc
     7321 tacccgcccc cctgagagga aaagctgtga tttggggctg gcaaaattcg cgcagattgt
     7381 ctgcctgcac agcattttgc aggaggaagc cgcaaaaccc tcagcggtgg cggggtaaag
     7441 gtgacgacgc tttgcgcgca aatttccctc ttgggagggg caatgctttt gcacaaacgc
     7501 cctcttgcgg ctctttttat gctctgcttc cactgcctcg cttgggcgag aaaaggggat
     7561 gatacacatc ccgtgcctct tttatgaaac ggggcaacgc ctgccgccag ccggaaatcg
     7621 ggggagcatg aagcacttcg tcaaaacccg gctcacgccg cgctctgtgc gaatccggcg
     7681 agagcaaaag cgggccggtg cgttgcacgc cggcccgatt tttgcgcctg ccgcccggct
     7741 ctgccgcgcg gcgctcccgt caagtggagc agttttttta ttcaggaaag ggattccccc
     7801 tcctcttttt ccgcggcgaa ataggcgatg ccgcaggcgc cgggcccggc gtgggtgccg
     7861 atcacggtgc cgatgggcga gaccggcggc tcgggcaggt gcagggtctc ggtgatatag
     7921 cggtagaacg gctccaccgt gcgccggtgc ccagtgtatc
//
LOCUS       DBMDYA010000030         7897 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2426_length_7897_cov_1.690226, whole genome shotgun sequence.
ACCESSION   DBMDYA010000030 DBMDYA010000000
VERSION     DBMDYA010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_7897_cov_1.690226"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            57..335
                     /locus_tag="ACZPFK_01395"
     CDS             57..335
                     /locus_tag="ACZPFK_01395"
                     /inference="COORDINATES: protein motif:HMM:NF046467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HIR1404994.1"
                     /translation="MRSASSSNFRTTGGQKNHTLTTAEIPSHAHQTEITYLTIYGSTD
                     AGQGKITGTRIDISGTGTRKINSTAAGGGQAFEIMPPYVAVCVHVRAG"
     gene            complement(332..2029)
                     /locus_tag="ACZPFK_01400"
     CDS             complement(332..2029)
                     /locus_tag="ACZPFK_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404995.1"
                     /translation="MAKTVQIINKFVNFEDGHRHTQGESNVEIYDITGPRYHDNIDLL
                     SLTWFVRATHPNYDTLINKQVVCAESSTDPEQICITWSVDGDFTAYAGELQVQFVGKS
                     ESGQEIIKLQSSGLMIKASVEGAASPPQDMFEQTLAQMEQLTNDAANAAAQSKLDADR
                     AGDAQEGAAQSAQAAQEAQQAVAGDVAQAGELVGQIQGAAAAAAASAAAAKKSETAAV
                     ASKTAAAGSASAAKNSETNAATSETAAANSAAAAKASENAASKSQTAAADSAAAADGS
                     KSAAQASAAAAAASETAAAGSAHAAQSSEAAAAQSAAAAADAAAAADGIHEINGKTGR
                     VVELTPADIKAVAAQGGEIGQTIVTYGGTDTAAPAPGDSMATTMQKMTAMYNRMVAAE
                     AALPPIGDWTLAAYRITYPGDDVNASTYPWVNFDDDLLPEKYPKLYALYGATLSVGAP
                     SGYFNLKKLADRFPLVSSSNFGDTGGAATHALTRAELPAGISGTVVLHSISIGTNIQS
                     ATGVFSGGAKNQKYRAGGDLIDGATSLGSINYNLGGSGTPIDIRGPFARVCGHVRAG"
     gene            complement(2039..2629)
                     /locus_tag="ACZPFK_01405"
     CDS             complement(2039..2629)
                     /locus_tag="ACZPFK_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404996.1"
                     /translation="MIESYQEEAALLPAAPPGQSFAVVGGIYDDGIALIFNGTAQESL
                     KHYKCNASIPFKVGQRVRIIEDSGTYVVEYPVGAPSTVLDADTAIRAAYATEAGYAQT
                     AGSAATAQTAAQATTAGTATVAQKAENADLAVNARAAEVAETATTAKDAETAKTALSV
                     DNTDLGYADIEFRAPYTNQLQYRIKGQTQWNTLQNG"
     gene            complement(2626..3657)
                     /locus_tag="ACZPFK_01410"
     CDS             complement(2626..3657)
                     /locus_tag="ACZPFK_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404997.1"
                     /translation="MAEVSVEYIVIRNGVEFAQLHAYRGGGGSIAVTSDAKIKWTLSG
                     KFEPDSRVNYLTDRIRPYLSIDGVRRPLGLFVPTDASVEDSGRRSVQPLTAYDITYLA
                     KSSKIENRLHLAAGTLYTSAIQMLLVESGITDILIEPSEAALQTDREDWEPGTDRLTI
                     ANALAAECNYNSLWMDQEGVVRGTLIRQPSAEAVTVIYRDDRYSLLYPEETRSIDLFD
                     HPNVIVVEVDNPDLETSMRAVSVNDRPDSPFSTVSMGRRIARYEKLDNIASQAELQVY
                     ADNKMFASMQATETRTWYTGPSAGHGVFDVVALYKGDISALYQETGWRLDLEAPYRMT
                     HTGKRVLYL"
     gene            complement(3650..6280)
                     /locus_tag="ACZPFK_01415"
     CDS             complement(3650..6280)
                     /locus_tag="ACZPFK_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404998.1"
                     /translation="MGTYTLICNASTRVGVYGDPASPATGVELVTSTAEGTTVALGFP
                     DVPANVVGFRVTSLQALNICMATNVDGDHQINIQIGYSKSIKVTEGGKGPGTHLDAIK
                     YLYGITTENASTPMQWRTFGAGAVFNRQMADNGISISALATHPTQGHNAVVRTNHSAE
                     KPYMVLNTEPVRVSAQDLSPAAEKQIYKGFVQRFSWSTAYDGTNVFGGVSQASARFFY
                     RGAGETVYKYKQINGNRNYIDFDTTEISADSGMDWYIEITSNSGVIDSSDVVSNVFRQ
                     TTVRLTSLSPSSSSKTYKGFAITFRWALNYSVPTGVSGALSQRSAILRWRKKDTTETT
                     SYAITSAAVEYTLGGSILPVGGIEWQVEVINTSGSSTMSDWISFTNIELPIVPADLYP
                     AEGGKILKGEINRFGWSIEVDGADKAPGEIVQTSAVLRYRPLGQSDTIEIQIDGAQSY
                     YDMPAGSITADDIEWQVQVTASTGTSGTSDWVHVNTQDALSTPACIFPVGVVIDDTAG
                     ITFVWQHIISTGTAQTAYELQYSTNLGSSYTTLATAQNGDTRYDTAPGMFPQGTLMWR
                     VRTKNGDGVWGGYSAAATIIIRRAPSAPIITYTDAKPRPTVRWQSEDQQGVRIQVGEH
                     DTGWLHSTAKEYQMPYILPDGIYRVALAIKTIFGIESAPAVTTVTIKNVPGPDLKAEV
                     KARLNAVYVNWVKMPEYTEYYVLRDGVPIARTPGGSYADALSVGKHVYAVQGMTADGY
                     YGQSDDLHAYLQISNAVISAVDKIDWLPLRLRRGERPAHHESRTPDIELAYYAGRTRP
                     EAYVSPMLEASHELTFSVRTRGDYARLKALQGRMVVYKDCWDDLIVGILGEVTAQLQR
                     AIDVTFVITETDFVEAVRYG"
     gene            complement(6284..>7897)
                     /locus_tag="ACZPFK_01420"
     CDS             complement(6284..>7897)
                     /locus_tag="ACZPFK_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1404999.1"
                     /translation="PLAVIVNQQAELANCEREHERVAERYGETSSQALKLEKRMLNLE
                     ASIEKNVKKSDDFAAALSDAEKVLDETSKSAEKAGKAAKEAGSGMEEGGKGAKTFDVA
                     LGTLIGNGLSAVIAKCSDLLNQTKELRRDLSFLAQNASDAGISMELMHDKAAELYAVT
                     GDTNEVVEALSNILATNLNADKSAEAIDLLAGAVVKFPETMKIESLADSLQETIATGE
                     ATGQYAELLGRLGVDVDKFNEKLGRASNEAGRQRIAIQALKREGLDQLWDSYKSNNET
                     LIDAARANYDLQKTYADLAVAIEPIETKIKTTFMQILIDHEDQILSIVDAVGGIIEIG
                     AGLLSFLSQLNPAVLLIGGALAMVVVKAAGVALGTKMMAGGVSAATKTLAAAGPQAAA
                     VGSQFLMLAVDIMIVAAAVALAAAGIAMLVAAIKGVPLVMQVPQVSNVGDLRSQVSQG
                     YADGTSAATPGWRWVGEHGPELMRFGGGEQVYTASQSRALASAGGGSGAYTYVDQRQY
                     IFKVDDVEDYVAIKRMHENEKMSIRQGRV"
BASE COUNT         1519 a         2400 c         2110 g         1868 t
ORIGIN      
        1 acccctcaca ataacatgcc gccctacatc acagcccgcg cccatgtccg ggccgggtga
       61 ggtctgcttc ctcctctaat tttagaacca cgggaggcca aaaaaatcat acccttacca
      121 cggcggaaat accctcccat gctcatcaaa cagagattac ctatcttact atctatggct
      181 ctacagatgc aggccaaggc aaaataacgg gcactcggat agatatcagc gggacaggaa
      241 caagaaagat aaacagcacc gccgccggag gggggcaagc gtttgaaatt atgccgccgt
      301 atgtggcagt ctgtgtccac gtgcgggcgg gttaacccgc ccgcacatga ccgcaaaccc
      361 gcgcaaatgg gccgcggata tcaataggtg taccgctgcc gccgaggttg tagttaatcg
      421 accccagtga ggttgcccca tctatcaagt ctcctcctgc ccggtatttt tggtttttgg
      481 cgcctcccga aaatactcct gttgcggact ggatgttggt cccgatgcta atcgaatgca
      541 ataccaccgt cccgcttatt cccgcaggca gctctgccct tgtgagggcg tgggtcgcgg
      601 ctccccccgt gtccccaaaa ttagaggagg aaacaagcgg aaagcgatcc gccagttttt
      661 tgaggttaaa atatccgctc ggtgccccga cggacagggt tgccccatac agtgcataca
      721 acttcggata tttttccggc agcagatcat cgtcaaaatt gacccaggga taggtactgg
      781 cattgacatc gtcgcccgga taggtgatgc gatacgccgc cagcgtccag tctccaatgg
      841 gaggtaatgc tgcctccgca gcaaccatgc gattatacat tgcggtcatt ttctgcattg
      901 tggtagccat gctgtctccc ggggccggtg cagctgtgtc ggtcccccca taggtgacga
      961 ttgtctgccc aatctccccc ccctgcgccg ctacagcctt aatatctgcc ggcgtaagct
     1021 ccactacgcg ccccgttttg ccgttgatct catggatgcc atcagcagca gccgctgcat
     1081 ccgccgcagc tgcagcactt tgggcggcgg ccgcctcgct ggactgcgcg gcatgggcgc
     1141 ttccagctgc tgcggtttca ctggctgcag cagcagcggc actggcctgt gctgccgact
     1201 tgctcccatc tgcagctgcc gcgctgtcag cggccgcagt ctgactttta gacgctgcgt
     1261 tctcgcttgc cttggcagct gccgcactgt ttgccgcagc tgtctcgctg gtagccgcgt
     1321 tggtctcgga gttttttgcc gccgatgcgc tgcccgccgc ggcggtcttg ctggcaactg
     1381 ctgcagtctc ggacttcttt gctgccgcag cactcgccgc cgcagctgcg gcagcgccct
     1441 gaatctgtcc caccagctcc ccggcctggg ccacatctcc ggccacagcc tgctgcgcct
     1501 cctgcgctgc ctgtgcgctt tgcgcagcac cctcctgtgc atctccggcc cggtctgcgt
     1561 ccagtttgct ctgtgccgcc gcgttggcgg catcattcgt cagctgctcc atctgcgcca
     1621 aggtttgttc aaacatatcc tgcggtggag acgcggctcc ttccacactg gccttgatca
     1681 tcaaaccgct ggactgcagt ttgatgattt cctgaccgct ctcgcttttg ccgacaaact
     1741 gtacttgcag ttctcctgca taggcggtaa agtctccatc tacggaccaa gtaatgcaaa
     1801 tctgctccgg gtcagtcgat gattcagcac ataccacctg tttgttgatg agcgtatcat
     1861 aattgggatg ggtcgcccgt acaaaccagg taagagataa aaggtcaata ttgtcgtgat
     1921 atctgggacc agtgatatca taaatttcga cgtttgattc accttgagta tgacgatggc
     1981 catcctcaaa gttgacaaat ttgttgatta tctgtactgt ttttgccatg atttcaccct
     2041 atccattctg cagcgtattc cattgggttt gtcctttgat gcgatactgt agctgattgg
     2101 tatatggagc cctaaattcg atatccgcat aaccgaggtc tgtattatcc acacttaacg
     2161 ccgtttttgc agtttccgca tcctttgctg ttgtcgccgt ctcggctacc tccgcagcac
     2221 gcgcgttaac agcgaggtcc gcgttttcag ccttttgagc cactgtggct gtaccagcgg
     2281 tggttgcctg tgctgcagtt tgagccgttg ccgcgctgcc cgcagtctgt gcgtatcccg
     2341 cctctgtggc ataagctgcc ctgatcgcag tatcagcgtc taggacagtg gatggagctc
     2401 ctacaggata ctccaccaca taggtcccgc tatcctcgat aatccgcacc cgttggccga
     2461 ccttaaacgg gatgctggcg ttacacttat agtgctttag actttcctgt gccgtgccgt
     2521 taaagatcag agcaatgcca tcatcatata tcccgcccac cacggcaaaa gactgcccgg
     2581 ggggagcagc gggcagcaaa gccgcctctt cttgataact ctcgatcaca gatataacac
     2641 cctctttcca gtgtgggtca tgcggtaggg agcctccagg tccagtcgcc agccagtctc
     2701 ttggtacagc gcggagatat cccccttgta cagcgcgacg acgtcgaaga cgccgtgccc
     2761 ggcggatgga ccggtgtacc aggtacgggt ctcggtcgcc tgcatgctgg caaacatttt
     2821 gttgtcggcg tagacctgca gctccgcctg agaagcgata ttgtccagct tttcgtatcg
     2881 ggcgatgcgc cgccccatcg agacggttga aaatgggctg tccggcctgt cgttgacgga
     2941 tacggcccgc atggaagttt caaggtccgg gttgtcaacc tccaccacaa tcacattcgg
     3001 atggtcgaac aggtcgatag accgtgtctc ctcgggatag agcaggctgt agcggtcgtc
     3061 ccggtagatc acggtgaccg cctccgccga cggctgccgg atcagcgtcc cgcgcaccac
     3121 cccctcctga tccatccaga ggctgttgta attgcattcg gcggcgagcg cattggcaat
     3181 cgtcagccga tccgtgcccg gctcccagtc ctcccggtct gtctgcaatg ccgcctcgga
     3241 cggctcgatc aggatgtccg tgatcccgct ctccaccagc agcatttgga tggcgctggt
     3301 gtagagcgta ccggcggcaa gatgcaggcg gttctcgatc ttgctggact tggccaggta
     3361 ggtaatgtca taggcagtta gcggctgcac gctgcggcgg ccgctgtcct ccaccgaggc
     3421 gtcggtgggc acaaacagcc ccaaaggccg tcgcaccccg tcgatggata ggtaaggacg
     3481 gatgcggtcc gtcaaataat tcacccggct gtccggctca aatttgcccg acagcgtcca
     3541 tttgattttc gcgtccgacg tcaccgcgat agagcccccg ccgccccgat aggcgtgcag
     3601 ttgggcaaac tccacgccat tacggatcac gatgtactcc accgatacct cagccatagc
     3661 gcaccgcctc cacaaaatct gtctctgtaa taacaaaggt gacgtcgatt gcccgctgca
     3721 gctgcgccgt cacctcgccg aggatgccta caataagatc atcccagcag tccttataca
     3781 cgaccatccg cccctgtaag gccttgagcc gggcgtaatc cccgcgtgtg cgcacactga
     3841 acgtcaactc gtgcgaggcc tccagcatgg ggctgacgta agcctccggc cgggtgcgcc
     3901 cggcgtaata ggcgagctct atgtccggcg tccggctctc gtggtgtgcg gggcgctcac
     3961 cacggcgcag ccgcagtggc agccagtcga ttttgtccac cgcgctgatc acggcgttgc
     4021 tgatctgcag atacgcatgg agatcgtcgc tctggccgta gtagccgtcg gcagtcatcc
     4081 cctggacggc gtagacgtgc ttgcccaccg agagagcgtc cgcatagctt ccgcctgggg
     4141 tgcgggcaat cgggacgcca tcgcgcaaaa cataatactc agtatactca ggcatcttca
     4201 cccagttcac gtaaacagcg tttaagcgcg ctttaacctc cgctttaagg tctggccctg
     4261 ggacattttt gattgtgact gtggtcactg ccggcgcgct ttctatcccg aaaatagtct
     4321 tgatagctaa ggccacgcga tatatgccat ctggcagaat atacggcatt tgatattcct
     4381 tggcggtgct gtgcagccag ccggtatcat gctccccgac ctggatgcgg acgccttgtt
     4441 gatcttcaga ctgccagcgg accgttggcc ggggcttggc gtccgtgtag gtaatgatgg
     4501 gagccgacgg ggcgcgtcgg atgatgatgg tggccgccgc gctatagccg ccccatacgc
     4561 cgtcgccgtt tttggtcctg acccgccaca taagcgtccc ttgggggaac atgccggggg
     4621 cggtgtcata tcgggtatcc ccgttttgtg cggtggccag ggtcgtatag ctgctgccca
     4681 ggttcgtgct gtactgcagc tcataggctg tttgcgctgt gccggtggag atgatatgct
     4741 gccacacaaa ggtaatcccc gcggtgtcgt ctatcacgac tccgacaggg aagatgcacg
     4801 ctggagtcga cagcgcatcc tgagtattaa cgtggaccca atcgctggta ccgctggtgc
     4861 ctgtggaagc agtgacctgc acctgccact caatatcgtc cgctgtaatt gatcccgcag
     4921 gcatgtcata ataggactgc gccccgtcga tctggatctc tatcgtgtca ctttgcccca
     4981 gggggcggta ccgcaatacc gctgaggtct gcacgatctc cccgggagct ttgtcagcac
     5041 catctacctc aatagaccat ccaaaccgat tgatctctcc tttgagtatc ttaccgccct
     5101 ctgcaggata aaggtcagca ggtactatcg gaagttctat atttgtaaag ctaatccagt
     5161 cgctcatggt cgaactgcca ctggtattga tgacttctac ctgccattca attcccccga
     5221 caggcagtat gctaccgccg agggtatact cgaccgctgc agaagtaatt gcatagctgg
     5281 tagtctctgt cgtgtccttt ttgcgccagc gcaatatcgc gctgcgttgg ctcaaagcac
     5341 cacttacgcc ggtaggcacg gaatagttta gtgcccatcg aaaggttatg gcaaagccct
     5401 tataggtctt gctgctgctg gacggagata ggctggtaag gcgaaccgta gtttgacgaa
     5461 aaacgtttga gactacatcc gaggagtcga ttactccact attggacgtt atctcaatgt
     5521 accaatccat acctgaatct gcagaaatct ccgtagtatc aaaatcaata taattacggt
     5581 ttccattgat ttgcttatat ttgtagacgg tctcgcctgc gcctcgatag aaaaaacgtg
     5641 cagatgcctg gcttaccccg ccaaaaacat ttgtaccatc ataggctgtt gaccaactga
     5701 atcgctgaac aaacccttta tatatttgtt tctccgccgc aggagacaaa tcttgtgccg
     5761 aaacccttac aggctccgta tttaatacca tatacggttt ttcagcagaa tggtttgttc
     5821 gaacaaccgc attgtgccct tgcgtcggat gagttgccaa tgcagaaata gaaatcccat
     5881 tatcggccat ttgtcggtta aaaaccgcac ctgcaccgaa agttcgccat tgcatcgggg
     5941 tgctcgcatt ttcggtggtg attccgtata gatatttgat agcatccagg tgggttccgg
     6001 ggcctttacc accctctgtc actttaatgc tttttgagta gccgatttgt atatttatct
     6061 gatggtcccc atcaacattc gtggccatgc agatattaag tgcctgaagc gatgtcaccc
     6121 gaaatccaac tacatttgcc gggacatccg ggaatcccaa ggcaacggtg gttccctctg
     6181 cggtactggt gaccaattcc actcctgttg caggagatgc gggatcacca tagacaccaa
     6241 ctcgcgtaga ggcattgcag atcagtgtat aggtccccat gctttaaacc ctcccttgcc
     6301 gtatgctcat tttctcgttt tcgtgcatgc gtttgatggc cacataatcc tccacatcgt
     6361 cgaccttaaa gatatactgc cgctgatcca cataagtata tgcgccgctg ccgccgccgg
     6421 cagacgccag cgcccgcgac tgggatgccg tgtagacctg ctcgccgccg ccgaaccgca
     6481 tcagctccgg cccgtgttct ccgacccagc gccagccggg cgtcgctgcg ctggtgccgt
     6541 ctgcatagcc ctggctgacc tgtgatcgca ggtcgccgac gttggagacc tgcggtactt
     6601 gcatgaccag cggcacaccc ttgatcgcgg caaccagcat ggcaatcccg gccgcagcga
     6661 gggcaacggc ggcggccacg atcataatat ccaccgccag catcaggaat tgagagccta
     6721 cagccgcggc ctgcggacct gcggccgcga gggtttttgt cgcggctgag acgccgccag
     6781 ccatcatttt agtgccaagc gcaacccctg cggctttgac aacaaccatc gccagtgcgc
     6841 cgccgatcag gagcacggcg gggttgagct gggacaaaaa gctcagcagg cccgcgccga
     6901 tctcgatgat cccgcccacc gcgtcaacaa tggacaggat ctggtcctca tggtcgatca
     6961 atatctgcat aaaggtcgtc ttgatcttgg tctcgatggg ttcgatggcc acggccaggt
     7021 cggcgtaggt cttttgcaga tcgtagttgg ccctggcggc gtcgatcagg gtctcgttgt
     7081 tggatttgta gctgtcccac agctggtcca gcccctcgcg cttgagcgcc tggatggcga
     7141 ttctctgcct gcccgcttca ttggacgccc gtcccagttt ttcgttgaat ttatcgacgt
     7201 ccaccccgag ccggcccagc agctccgcat actgccctgt ggcctcgccg gtggcgatgg
     7261 tctcctgcaa gctgtccgcc aggctttcga ttttcattgt ctccgggaac ttgaccaccg
     7321 cgcccgccag caggtcgatg gcctcggcgc tcttgtccgc gttgagattg gtggccagga
     7381 tattgctcag ggcctccact acctcgttgg tgtcgccggt gacggcgtac aactccgcgg
     7441 ccttgtcgtg catcagctcc atgctgatcc cggcatctga tgcgttttgg gccaaaaatg
     7501 agagatcccg gcgcagttct ttcgtttgat tcaacaggtc ggaacacttg gcgattactg
     7561 cactcagccc gttgccgatc agggtaccca gcgcaacgtc aaaggttttc gcgcccttgc
     7621 cgccttcctc catgccgctg ccggcctctt ttgcggcctt tccggctttc tcggcactct
     7681 tagaggtttc gtccagcact ttctccgcat ccgacagcgc cgctgcaaaa tcatccgatt
     7741 ttttaacgtt cttctcgatg gatgcctcca ggttcagcat ccgtttttcc aacttcagcg
     7801 cctggctgct ggtctcgccg taccgctccg ccacacgctc atgctcccgc tcgcagttgg
     7861 cgagctctgc ctgctggttc acgatgacgg cgagcgg
//
LOCUS       DBMDYA010000031         7767 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2472_length_7767_cov_1.575789, whole genome shotgun sequence.
ACCESSION   DBMDYA010000031 DBMDYA010000000
VERSION     DBMDYA010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_7767_cov_1.575789"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(431..982)
                     /locus_tag="ACZPFK_01425"
     CDS             complement(431..982)
                     /locus_tag="ACZPFK_01425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1405000.1"
                     /translation="MQCSVQAGEGVLAYELTRKKVKNLNLRVRPDGSLAVSAPRWAAR
                     AEIDRFVASRAAWVERTRARLAQRSPAPEPPACALTDEQCMAVFQPFLDRYYPIFAPA
                     IGGGLEIKLKTLKSLWGVCRPAKRQITLNRRLAEQPAEAVEYVVLHEYLHFWHPDHGK
                     GFHRELDRLMPDNRSRRALLPRR"
     gene            1195..2025
                     /gene="nudC"
                     /locus_tag="ACZPFK_01430"
     CDS             1195..2025
                     /gene="nudC"
                     /locus_tag="ACZPFK_01430"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR1405001.1"
                     /translation="MIQDLFPRRFHTEYRPTAPGPNSVVLWFEGENVLARQAEGRLEF
                     PRFEDCPGLAPDQLCYLFRIDEEEFFLCPGADAPPPQGFLFVENEVFRHGLRPKAHAF
                     AGLTALQVARWYRDNRFCGRCGGPMRHSARERMVLCPRCGNTVYPKISPVVIVGVRDG
                     ENLLMTRYAGRSYGGYALIAGFIEVGETPEDAARREAFEETGLRIKNLSYCASQPWGY
                     SDSLLLGFFADLDGDGAITLEEDELSMAQWVPRGEIAVQPDDFSLTNHMICLFRDGKA
                     "
     gene            complement(2837..3664)
                     /locus_tag="ACZPFK_01435"
     CDS             complement(2837..3664)
                     /locus_tag="ACZPFK_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR1405002.1"
                     /translation="MTVLWIVLAAVAVLALLALFLLWPGRPSAEKTAPFYGRNYAHRG
                     LHSRDKSVPENSLTAFRLAAEAGYGIELDIQLSKDGQVVVFHDDTLDRVCGVHGRVDA
                     FTLEELRGMRLCGTDERIPLLTEVFAVVAGRSPFIIELKTGPRNAELCEKGLALMRAY
                     DGPYCVESFDPRIVGWFKKHAPDLLRGQLAGPPKTYDNMPAAGGLVLGNLLGNVLARP
                     EFIAYDNKPKPACVRFVERHGPVKVLWTARDEADRERVERENDLVIFEFYRPQVRFK"
     gene            4074..4754
                     /locus_tag="ACZPFK_01440"
     CDS             4074..4754
                     /locus_tag="ACZPFK_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1405003.1"
                     /translation="MIQILIVDDEPRIRQLIREYLEHEGFSCTEATDGSAALAQLSSA
                     SFDLVLLDIMMPFVDGMTCLKEMRARKLSTPVIMLTARGEEYDRVAGLEGGADDYIVK
                     PFSPREMVARVKAVLARTLPKEQSDSEIFVFGDLVIDTASHSVKIAGEEISLTPKEFD
                     LLVFLAHNKGIALSREKILQKVWNYDYYGEDRTVDTHIKMLRSHLGSHREDIATVWGV
                     GYKFDPAN"
     gene            4765..6357
                     /locus_tag="ACZPFK_01445"
     CDS             4765..6357
                     /locus_tag="ACZPFK_01445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405004.1"
                     /translation="MKSIKTKLALFVSCLCIVLILLVWLLTVALFESNYTRSIRKELS
                     TQIASVAAVLESEDARDASLGELFQQIQPLVKTGVCVDVSATDACIARRGYLSSGGLP
                     LNSYRALFVLEGIGDGCQLHPSSSQGFWGEQREWDSGDVIFWRQMTAQEGRLNKTLFD
                     QRTAPYRQQQVMGLLTSSGYIVLASTNLERVDQAMSVVSNQLILITVILLAVSIVSAL
                     LFARWFTRPVTQLSSAAKQVAAGNYDVAVDIRSEDELGALARDFNYMTREIAKSTQLQ
                     RELIANISHDLRTPLTLIKGYAETIRDLDGEDKARRDADLSIIVDEADRLSVLVNSVM
                     ELSKYSSGAARPTPSRFDLAELAADVLRRYADRAEKERFSLHQDGLAEAEVVADPVQI
                     GRVLHNLVSNAIPHLPQGGCVTVRLTARAGAVRAEVADNGSGIEEKELPFIFDRYYRA
                     RSDAGKRGSGLGLSIVKAILVAHGAPFGVQSAVGVGSTFWFELPVPAAPPALPLKAPK
                     DPRQAARRDPKKAEEPEDVKRK"
     gene            complement(6734..7456)
                     /locus_tag="ACZPFK_01450"
     CDS             complement(6734..7456)
                     /locus_tag="ACZPFK_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1405005.1"
                     /translation="MSHSIANAPGERSFAHGKNWYKIFWVFFIGCLIGYGCEMVFAYL
                     KEGYWVERTGLIYGPFNQIYGLGAVIFMLTLYHIRRANAGIIFLASAFSGAAFEWVCS
                     WVQEQVFHSVSWEYSDTPANLGGRTNLFFSICWGLMGMIFIRNIYPTLSKIIESIPNR
                     IGKPLTWCLAVFMAFDILISALAVARWAERTYGYDVPPTNAVNAFLDEHYPDEFMSAT
                     YTSMEFRKPDDPPPRPAEEKAD"
BASE COUNT         1331 a         2659 c         2554 g         1223 t
ORIGIN      
        1 cgggcccggt tccccggcgg tccgtctccc gctccaggcg cggccgccga ggcggtcagc
       61 ctcaaaaacg gctcagaggt atttttttat tttaccacat caggggggat tttccaacca
      121 aaggccgtcg gaacggcccg accggggtcc gccccctccc cgaggccccc aaagggaggc
      181 gaacggcgca aaccccggcg gtccggtggc tccgccgacc gggttcctgc gtcagaggca
      241 cgaacagggg cagcgaacgg gggcagaagg cgaccgggca gctgtgccgg tctgcccggg
      301 gccgccccca ccccggcccg gcgggcttgc acccgcggct tgaggccgat aaaaagagct
      361 ttgccgtggc ccgcaaccga cagccgcccg gcggcccggc cccccccgcg ggccaggccc
      421 gtgccgccgc tcagcgccgg ggcagcaggg cgcggcggct gcggttatcc ggcatcagcc
      481 ggtccagctc gcggtggaac cccttgccgt gatccgggtg ccagaaatgc aggtactcat
      541 gcagcaccac atactccacc gcttccgccg gctgctcggc caaacggcgg ttgagggtga
      601 tctgccgctt tgccgggcgg cagacccccc acagcgattt cagcgttttc agcttgatct
      661 ccagcccgcc gccgatagcg ggggcgaaga tgggataata ccggtcgaga aagggctgga
      721 ataccgccat gcactgctcg tcggtgaggg cgcaggccgg gggctcgggg gcgggggacc
      781 gctgggccag ccgcgcccgc gtccgttcca cccaggcggc ccgggaggca acaaagcggt
      841 cgatctccgc ccgcgccgcc cagcgcggcg cgctcaccgc aaggctgccg tccggccgca
      901 cccgcaggtt gagattcttc acctttttgc gggtcagctc gtaggcgagc acaccctctc
      961 cggcctgtac gctgcactgc aacgctgccg ccccctctcc ggatcgtcct tgccgcgcgg
     1021 gccccgcggc ccgtcttttt cagcgtctat tatagcccgc ggcccctttt tcggcaagcc
     1081 gtaccggccg acgccccggc aaagaggcgc gctgacggcc gccttgccct gcgcgcccgg
     1141 ttctgctatg atacccttga aaggctttta tgccgggaaa ggaagcgtct ggtcatgata
     1201 caggatcttt ttccgcgccg gttccacact gaataccgcc ccacggcgcc ggggccgaac
     1261 agcgtggtcc tctggttcga gggggaaaac gtgctggccc ggcaggcgga ggggcggctg
     1321 gagttcccgc gctttgagga ctgtcccggc ctcgcgccgg atcagctgtg ctatctgttc
     1381 cgcatcgacg aggaggaatt ctttctctgc cccggcgccg acgcccctcc cccgcagggc
     1441 tttctctttg tggaaaacga ggtctttcgc cacgggctgc ggcccaaggc ccacgccttt
     1501 gcgggcctga ccgccctgca ggtggcccgc tggtaccgcg acaaccgctt ttgcggccgc
     1561 tgcggcggcc ccatgcggca cagcgcccgc gagcggatgg tgctctgccc ccgctgcggc
     1621 aacacggtct atcccaagat ctcgccggtg gtgatcgtgg gggtgcggga cggcgaaaac
     1681 ctgctgatga cccgctatgc cgggcgcagc tacggcggct acgcgctgat cgcgggcttt
     1741 atcgaggtgg gcgagacccc ggaggacgcg gcccgccgcg aggcatttga ggagaccggg
     1801 ctgcggatca aaaacctgag ctactgcgcc tcccagccct gggggtactc cgacagcctg
     1861 ctgctgggct ttttcgccga cctggacggg gacggtgcca tcacgctgga ggaggacgag
     1921 ctgtccatgg cccagtgggt gccccgcggc gagatcgccg tgcagccgga cgacttcagc
     1981 ctgaccaacc acatgatctg cctcttccgg gacggcaagg cctgaaaccg cgttcggccg
     2041 ccgacgctgc cgggccgatc tgcctacagc taagtccgga tgcagtaaag cgcggccgga
     2101 cacggccgaa gccggatgcg gcagcatgcg ggaggtcgtc gccgaacggg gtagaacgca
     2161 gccaaagccg gatacagcca aacgcgaccg gacgcagccg gacagggccg cagccggaca
     2221 gggccacagc gggatgcggg aaaacgtcgc gggaggcggc agagcgcggc aaggcaaaaa
     2281 gaaacggccg gggcgcgctc cggcacagcc cggcccgcgg cgttggtccc gcatcccgcc
     2341 aagcccgcac ggcggcgcgc ttgcttcccg gtccgggcgc ggccaagggc ggtggggctg
     2401 caaagccgca aaaagaaaag ccgcagctcg gcaagcgtcc gcctccggcc cgccgccgct
     2461 ctggaggctc tccccccggg gatcccgtcc cgcggggtga agcccccctt tcccgctcaa
     2521 aaccgaaaaa aaccggcgca gctccaccct gcgccggttt ttgttcttct cttttgtccc
     2581 cgcgtgtccc cccgcgtacc cgcaggttaa agcgctgggc aaggcgtcgc acctcgcaaa
     2641 ggcgaaagtg acaaaccgca gaggcccggg gaagccaagc gggttccccc gcttttttcc
     2701 ctcgcctttc tccgccgctt tccaggccgg ccccgcgaaa aagccgaagc ggtaaatcgg
     2761 gcctcggccc cgcagggccc gggtgaaagg cgtggcccgg cggcatcgtc ttctccgcac
     2821 ctcggccgca gatccgctat ttgaaccgca cctgggggcg gtagaattcg aagatcacca
     2881 gatcgttctc ccgctccacc cgctcccggt ccgcctcgtc gcgggcggtc cagagcactt
     2941 tcaccgggcc gtgccgctcc acaaagcgca cgcaggcggg cttgggcttg ttgtcgtagg
     3001 cgatgaactc ggggcgggcc agcacgttgc ccagcaggtt gcccagcacc aggccgcccg
     3061 ccgcgggcat gttgtcatag gtcttgggcg gccccgccag ctggccgcgc agcaggtccg
     3121 gcgcgtgttt tttgaaccag cccacgatgc gggggtcaaa gctctcgaca cagtaggggc
     3181 cgtcataggc gcgcatcagc gccaggccct tttcacagag ctcggcgttg cgcggcccgg
     3241 tcttgagctc gatgataaag ggcgagcgcc ccgccaccac cgcgaacacc tcggtcagca
     3301 gggggatgcg ctcgtcggtg ccgcaaaggc gcatgccgcg cagctcctcc agcgtgaacg
     3361 cgtccacccg cccgtgtacg ccgcagaccc ggtccagcgt gtcgtcgtga aagaccacca
     3421 cctggccgtc tttggacagc tggatatcca gctcgatgcc gtagcccgcc tctgccgcca
     3481 gccgaaacgc ggtgaggctg ttctccggca ccgacttgtc ccgcgagtgc agcccgcggt
     3541 gcgcgtagtt gcggccgtaa aagggggcgg ttttttccgc tgaggggcgg ccgggccaga
     3601 gcagaaacag ggcgagcagc gccagcacgg ccaccgcggc caggacgatc cagaggacgg
     3661 tcattgaacc tgcacttcct ttcggcggac acggccgccg tcgcttttgg ggccgccgtg
     3721 ggcggcccgc gccgggcggc gccgccgccc gccctcccat tgtagcgcgg cggggggccg
     3781 gtgtcaacgc gcccccgccc cagcggcaaa agcacaggta attttttcct gctccgccac
     3841 gccgccttgg tatttccccg gaaaactggt atcataaggg gcaggggagg cccggcggcc
     3901 gccgatgccg cgagaggcgt tttccgcctc cctccctctc cgcccagcct ctttccgggg
     3961 ccgggtcgca caaaagcaac agaactttca cacaattgac ataccggaaa attacactaa
     4021 aatttaatct accaatcgaa aaacacccgc aacgtcagaa tgaggtgaac cgtatgatcc
     4081 agatcctgat cgtcgacgac gaaccccgca tccgccagct catccgcgaa tacctggagc
     4141 acgagggttt ttcctgcacc gaggccaccg acggctcggc cgccctggcc cagctctcca
     4201 gcgccagctt cgatttggtg ctgctcgata tcatgatgcc gtttgtggac ggcatgacct
     4261 gcttaaaaga gatgcgcgcc cgcaagctgt ccaccccggt catcatgctc accgcccgcg
     4321 gcgaagagta cgaccgggtc gccgggctgg aggggggcgc cgacgactac atcgtcaagc
     4381 cgttcagccc gcgggagatg gtggcccggg tcaaagcggt gctggcccgc accctgccca
     4441 aagagcagtc ggacagcgaa attttcgtgt tcggcgacct ggtgatcgac accgcctctc
     4501 acagcgtcaa aatcgcgggc gaggagatct cgctcacccc caaggagttc gacctgctgg
     4561 tctttctcgc ccacaacaaa ggcatcgccc tctcgcggga gaagatcctg caaaaggtct
     4621 ggaactacga ttactacggc gaggaccgca ccgtggacac ccatatcaag atgctgcgta
     4681 gccatctggg cagccatcgg gaggacatcg ccaccgtctg gggcgtgggc tataagttcg
     4741 acccggccaa ctgaggggct gtgcatgaaa tcgatcaaaa ccaagctggc cctctttgtc
     4801 tcctgcctgt gcatcgtgct gatcctgctg gtgtggctgc tgaccgtcgc gctttttgag
     4861 tcgaactaca cccgcagcat ccgcaaggag ctgagcaccc agatcgcctc ggtggccgcg
     4921 gtgctggaga gcgaggacgc ccgggacgcg agcctgggcg agctgttcca gcagatccag
     4981 ccgctggtga agaccggcgt ctgcgtggac gtgtcggcca ccgacgcctg catcgcccgg
     5041 cgggggtatc tgtccagcgg cggcctgccg ctgaacagct accgcgccct ctttgtgctg
     5101 gagggcatcg gcgacggctg tcagctgcac ccctcctcct cccagggctt ttggggcgag
     5161 cagcgggagt gggactccgg cgacgtgatt ttctggcgcc agatgacggc ccaggagggc
     5221 cgcctcaaca agaccctgtt cgaccagcgc accgccccct accggcagca gcaggtgatg
     5281 ggcctgctca cctccagcgg ctacatcgtg ctggccagca ccaacctgga gcgggtggat
     5341 caggccatgt cggtggtcag caatcaactg attctgatca ccgtgatcct gctggcggtc
     5401 tccatcgtct cagcgctgct cttcgcccgc tggttcaccc gcccggtgac ccagctgtcc
     5461 agcgcggcca aacaggtggc ggcgggcaac tacgacgtgg cggtggatat ccgctcggag
     5521 gacgagctgg gcgccctggc ccgcgatttc aactatatga cccgcgagat cgccaagtcc
     5581 acccagctgc agcgcgagct gatcgccaac atcagccacg acctgcgcac cccgctgacc
     5641 ctgatcaagg gctatgccga gaccatccgc gacctggacg gcgaggacaa ggcccggcgg
     5701 gacgctgatc tttccatcat tgtcgacgag gccgaccggc tgtcggtgct ggtgaacagc
     5761 gtcatggagc tgtcgaaata ctcctccggc gcggcccggc ccacccccag caggttcgac
     5821 ctggcggagc tggccgccga cgtgctgcgc cggtacgccg accgggcgga aaaagagcgc
     5881 ttctccctac accaggacgg cctggccgag gccgaggtgg tggccgaccc ggtgcagatc
     5941 gggcgggtgc tgcacaacct ggtctccaac gccattccgc atctgcccca gggcggctgc
     6001 gtgacggtgc ggctgaccgc ccgggccggt gcggtgcggg cggaggtggc cgacaacggc
     6061 agcggcatcg aggagaaaga gctgccgttc atcttcgacc gctattaccg cgcccgcagc
     6121 gacgcgggca agcggggcag cggcctgggg ctctccatcg tcaaggccat cctggtggcc
     6181 cacggcgcgc cgttcggggt gcagagcgcg gtgggggtgg gctccacctt ctggtttgag
     6241 ctgcccgtcc cggcagcgcc gcccgccctg cccctgaaag cccccaagga cccccggcag
     6301 gccgcccggc gggaccccaa aaaggcggag gagccggagg acgtcaaaag aaaataaaaa
     6361 agagcttccg ctcaggcgga ggctcttttt ttggtctccc ggcccgcacc gcgcccgggg
     6421 cggccgagat caattgcttt ttcggctctc cgtttgaggg ccgcgcgggg gggaaagacg
     6481 ccgccggtgc gccggcggca ggggagaaac gccgcttacc ccgagggacc gcgcaagggg
     6541 caaaaagcgc ggctattttg gtcaaacccg gcccgcgcac gcgcgcgtgg gcccgcggcg
     6601 gccgccgtat gcccccaggg gagccggcgg ccgcaggtcg cgccgaaagg ttcgccgggg
     6661 cggcgaggcc gcgccgcttc tgccgccctg ctttcgcttc ccccggcgca gggtttcagg
     6721 gctgtccggc cgctcagtcg gctttttcct ccgccggccg cgggggcgga tcgtccggct
     6781 tgcggaactc catgctggtg taggtggcgc tcatgaattc gtctggatag tgctcgtcga
     6841 gaaaggcgtt caccgcgttg gtgggcggca cgtcatagcc gtaagtgcgc tcggcccagc
     6901 gggcgaccgc cagcgcggag atcaggatat caaaggccat gaacaccgcc aggcaccagg
     6961 tgagcggctt gccgatgcgg ttggggatgc tctcgatgat cttgctcagg gtggggtaga
     7021 tgttgcggat aaagatcatg cccatcaggc cccagcagat ggaaaaaaac agattggtgc
     7081 gcccgcccag gttggccggg gtgtcgctgt actcccagct gaccgaatga aacacctgct
     7141 cctgcaccca gctgcacacc cactcaaagg ccgcgccgga aaaggcgctc gccaaaaaga
     7201 tgatgcccgc gttggcgcgc cggatatgat agagggtgag cataaagatc accgcgccca
     7261 ggccgtagat ctggttgaac gggccgtaga tcaggccggt gcgctccacc caatagccct
     7321 ctttcaggta ggcgaacacc atctcgcagc cgtagccgat caggcagccg atgaaaaaca
     7381 cccagaagat cttataccag tttttgccgt gcgcgaaaga cctctccccc ggcgcgttgg
     7441 cgatagaatg tgacatgaca gctcctttga gaggaaaaat gggacacagg gcccgcgggc
     7501 cctggacggc ggagcgccgc ccgcgggccc ggatacctac aaattttaac acgttttcag
     7561 gggaaagaaa agtgcaggac gtgctatttg ctcttatttt tcggtatggg cgggaaacag
     7621 cgccacaaaa gcctttgccc ccgcgctgag cggcgcggtg ccgcgggtgg ccacggtgac
     7681 cgaggtgacc agctccggcg cagcgatctc ccgcacctcc agggcgccgg ggcagagggc
     7741 caccgccgag cggggcacca gcgccgc
//
LOCUS       DBMDYA010000032         7734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2481_length_7734_cov_1.675095, whole genome shotgun sequence.
ACCESSION   DBMDYA010000032 DBMDYA010000000
VERSION     DBMDYA010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_7734_cov_1.675095"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="ACZPFK_01455"
     CDS             complement(<1..137)
                     /locus_tag="ACZPFK_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015423.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HIR1405006.1"
                     /translation="MTLNELPIGESAVITAVGGGVIRDVFSREIPRILRQDVYAVASI
                     AG"
     gene            complement(286..1677)
                     /locus_tag="ACZPFK_01460"
     CDS             complement(286..1677)
                     /locus_tag="ACZPFK_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1405007.1"
                     /translation="MKSNRFSRAFMNKPGHLSPTRVIVASFCAVILFGTLMLMLPVAT
                     RDGQIALIDALFTATSATCVTGLIVYDTFTKFTLFGQAVILLLIQIGGLGLVTLTTFF
                     NLALRKKIGFKSMKLASESISLDEAAGAKKLIYVVMRTSLGFELIGAILLMGALVPRF
                     GARGVWMSVFTAISAFCNAGFDLFGSLGPYSSLVPFAGSWYVLVIVMLLIVCGGLGFI
                     VWQDLFSFYRRKKLTLHSKLVLIITVTLVVGGAFAIGAMEWDNAATMGGMTTPQKVLS
                     SLFQSVTCRTAGFNSIDQAGMHSISKFTCTLLMFIGAAPGGTGGGIKITTFTIVLLAV
                     AAVLRGREDPVLRERRIDKKTVYRALTITTLGLGVVICTTLVMVFNTPGRGGMIDGNL
                     VDCLYEAASAFGTVGLSVGVTGQLSALGKVATALTMLIGRVGPISMGMTLAARADELS
                     RREVLPEAKIVVG"
     gene            1862..1937
                     /locus_tag="ACZPFK_01465"
     tRNA            1862..1937
                     /locus_tag="ACZPFK_01465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Arg,seq:cct)
     gene            complement(2165..3022)
                     /locus_tag="ACZPFK_01470"
     CDS             complement(2165..3022)
                     /locus_tag="ACZPFK_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405008.1"
                     /translation="MPVDLIHEKEFASRLIRPDTWIITGHPVSAVTYLLIGGHTALVI
                     DPGEHVRNLRDYVRTITDLPLIVANTHAHGDHTACNGQFKEYPIYASAGAVEELGQKR
                     VDPNRYTTEVRYDYDYQALPIGDGHVFDLGGRRVRAIAAGCHSEGSLFYYDEKYQLLF
                     TGDELECGQVLIQGSIHGARHSVERYRDNLLKVKRAAGRVDLVCPEHNGSPADGSVLD
                     AYIENCERIMAGHEGGRKISSATYLGPDDPRGPERVRELLSDPYSRRSEWKGSALVYS
                     TNCVFLKDE"
     gene            complement(3058..4428)
                     /locus_tag="ACZPFK_01475"
     CDS             complement(3058..4428)
                     /locus_tag="ACZPFK_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405009.1"
                     /translation="MTTDRKRVGLDLTRGPIFRLLILFAAPMVLTNLVQQLYSTVDLI
                     VIGKFVGNVGTVGVATGGEMADFMTIFAVAIAMGGQIYIAQLAGAKEEKRLKEAVGTL
                     LTLLMGASVVVMAAALLFHRPILEMLNCPGEARGQAAQYMMITALGMPFVFGYNAVAS
                     ILRGMGESTRPLIFITVAAVINIFADLLLVAVFPLQAAGTAIATVLSQAGAFFAAFFY
                     MYRHREAFDFQLGWSFFRIRATPLKLILKMGVPQLARSFAVQGSMLWVKANINVYGLV
                     PSSTYSIGNKIEKFMNVFVMGVDGAAGAMIGQNLGAQRQDRVKKTMWYTLACNFSMAF
                     VVCAVFLLFPRQLFRLFTNEAQVIEFGVVFLRIMSVGMLVVAFASCFKSIATGAGAAG
                     LCFLLGILDGVCRILVCLLFFHVFGQGVQSYFWGAAFCMLVPGLISFFYFITGRWKKK
                     KLLSEL"
     gene            complement(4537..5550)
                     /locus_tag="ACZPFK_01480"
     CDS             complement(4537..5550)
                     /locus_tag="ACZPFK_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405010.1"
                     /translation="MTGQMWPIAGASRAKRLKWGSPQNQISWAYMKQLREKNGAMRVY
                     DVNPYVEVYRFRDNMYGLFNQNCDGAGDVWMYLILGPEKAMLVDTAYGLGDLKGLVDE
                     ITGGMPLIVVNTHTGPDHVLGNVRFDPVYCCDIEEQNIRNKCKPGAWDYLFDENGNNI
                     WLEFDRKDLPEYRDYELVGVPNGTTWDLGGGYEVELVWTGGHAAGHAMFLDKKGRCLF
                     AGDDVCSDVIGCGGGPRPGGVNNQYCNLSTYRDELAKLCRRLDEFDYIFPGHFVVNLE
                     NNLLLNILEALNQIIADPECYDYKVEHAGGQGGEKRVRMHKFVKGFGTIAYSANGVYP
                     PEG"
     gene            complement(5598..6605)
                     /locus_tag="ACZPFK_01485"
     CDS             complement(5598..6605)
                     /locus_tag="ACZPFK_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405011.1"
                     /translation="MRGQMWPIEGLSKAKRIKWDAPQNQISWAYMKELREHDKTRRVY
                     DVDPYIEVYQFRDNLYSLFHPNCDGMGDVWMHLIVGPEKAMLIDTGYGLGDLKGLVDE
                     ITGGKEIIVVNTHDHFDHAYGNCRFGRAYCHEYLVPYLQTQHAHMWDYLFDAFGNNIW
                     LDFDRKDLPVFEEYEIIGVPDGYTWDLGGGYEVELIFTGGHAAGHAAYLDKHNRILFT
                     GDNICSDVSGCGSVNVTRGNGPHQECTQLSFYRDRVKLLVDRMGEYDYIFPQHFMLNL
                     ENNLMPNILEALDSILADPANCDYKIESWGKDRTAKNERFFKFVKGFSVIGYGYKPTE
                     K"
     gene            6821..>7734
                     /locus_tag="ACZPFK_01490"
     CDS             6821..>7734
                     /locus_tag="ACZPFK_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1405012.1"
                     /translation="MDLVAFQYFLSVSQCLSFSEAAEQNHISQSSLSKAILRLERELN
                     VKLFDRNHHPISLTAAGLCLQEHLGQMRPLYVQAMRELDRYRVHRNIRCYFVPRLYAL
                     KNAALAFLSANRDIELTLETGSDFTTAVREMMEGDYDMAVMHQPFDLPEGVRMSMIYD
                     DKLYAVLPQNHPLAGREAVSLAELDGYTFVETNFSATIVQGLAAKFGFRPGGIDVHRS
                     ITGSREEVMLRIARSQNIGIFCSRDITLYNIQPLKLVTRPLKEISSLPIVLLEREGRT
                     DTEWHQRFCAFLKKEQENYAAPLSALPSG"
BASE COUNT         1436 a         2542 c         2341 g         1415 t
ORIGIN      
        1 ccggcgatgg aggccaccgc gtagacgtcc tggcggagga tgcgggggat ctcccgggaa
       61 aagacgtcgc ggatcacccc gccccccacg gcggtgatga cggcgctctc gccgatgggc
      121 agttcgttca gggtcatgcc atcctcccat ctgttagcct atgctaacaa gcaaagcaaa
      181 aaataggcgc ggcgcagaac gccgcacctc tatgataata cgcaatgtcg tccgcgtcaa
      241 taaaaccgcc gacattttgt gaaatttgtc gaaacgccgg ccaggtcagc cgaccacgat
      301 cttggcctcc ggcagcacct cgcggcggga cagctcatcc gcccgggcgg ccagggtcat
      361 gcccatcgag atggggccca cccggccgat gagcatggtc agggcggtgg ctaccttgcc
      421 cagggcgctc agctggccgg tgacccccac cgacagcccc acggtgccaa aggcggaggc
      481 cgcctcatac aggcagtcca ccaggttgcc gtcgatcatg ccgccgcggc ccggcgtgtt
      541 gaagaccatg accagggtgg tgcagatcac cacccccagc cccagggtgg tgatggtcag
      601 ggcgcggtag acggtttttt tgtcgatgcg ccgctcgcgc agcaccgggt cctcgcggcc
      661 gcgcagcacc gcggccaccg ccaggaggac gatggtgaac gtggtgatct tgatgccgcc
      721 gccggtgccg cccggcgccg cgccgatgaa catcagcagc gtgcaggtga acttggagat
      781 ggagtgcatg cccgcctggt cgatgctgtt gaagcccgcc gtgcggcagg tgaccgactg
      841 gaacaggctg gagagcactt tctgcggcgt cgtcatgccg cccatggtgg cggcattgtc
      901 ccactccatg gcgccgatgg caaaggcgcc gcccacgacc agggtgacgg tgatgatgag
      961 caccagcttt gagtgcagcg tcagtttttt gcggcgataa aagctgaaca ggtcctgcca
     1021 gacgatgaag cccaggccgc cgcagacgat gagcagcatg acgatgacca gcacatacca
     1081 gctgccggca aagggcacga gactgctgta ggggccaagg ctgccgaaca ggtcaaagcc
     1141 cgcgttgcaa aacgccgaga tggcggtgaa caccgacatc cagaccccgc gcgcgccgaa
     1201 gcggggcacc agggccccca tcagcaggat ggcgccgatc agctcaaacc ccaggctggt
     1261 gcgcatgacc acgtagatca gcttttttgc cccagcggcc tcgtccaggc tgatggattc
     1321 gctggccagc ttcatgctct tgaagccgat cttcttgcgc agcgccaggt tgaaaaaggt
     1381 ggtcagggtg acgaggccca ggccgccgat ctggatcagc agcaaaatca ccgcctgccc
     1441 gaacagggtg aatttggtga acgtgtcgta gacgatcagg ccggtcacac aggtggcgga
     1501 ggtggcggtg aacagggcgt cgatcagcgc aatctgcccg tcccgcgtgg cgacgggcag
     1561 catcagcatc agggtgccga agaggatgac ggcgcagaag ctggccacga tgacccgggt
     1621 gggggagaga tgccccggct tgttcatgaa cgcccggctg aacctattgc tcttcaactc
     1681 aattcctcaa ctttcaggcg cggacggcgc taaacgcgcc gcctttcgcc ggtcatagtt
     1741 ttaacacatt ctttatgggc cacatcggcc attataacac ccctttccgg cgctttcaac
     1801 ttgatttttt ccccgttctc cactataatg attcttgcgc cccaaagggc gcgccgcacg
     1861 ggcgtttgta gctcagggga tagagcattc gcctcctaag cgaagggccg cgcgttcgat
     1921 tcgcgccaag cgcaccatgt cgccgcgggc tcaggcccgc acaaagaaag cccccggctg
     1981 aaaagccggg ggctttcttt gtgcgggcac ggcggcgggg cctttttcgg ccatgccttt
     2041 cacatcccgt gtttctttcc ttattaaata gaagaacggt ctccggccgg ggcgagcccc
     2101 cggccggaga ccgttctttt tttgagtccg gcgccccaaa aacgcgcccc ctctccccgc
     2161 ggcgctattc gtcttttaaa aagacgcagt tggtggaata gaccagcgcc gagcccttcc
     2221 actcgcttct gcgcgaatag gggtcggaca gcagctcccg cacgcgctcc gggccgcgcg
     2281 ggtcgtccgg ccccaggtag gtggccgagc tgatcttgcg gccgccctcg tgcccggcca
     2341 tgatccgctc gcagttttcg atgtaggcgt ccagcacgct gccgtcggcg ggcgagccgt
     2401 tgtgctcggg gcagaccagg tccaccctgc ccgccgcccg cttgaccttg agcaggttgt
     2461 cccggtagcg ctccaccgag tggcgcgcgc cgtggatgga gccctggatc agcacctggc
     2521 cgcactccag ctcatcgccg gtgaacagca gctgatactt ttcgtcgtaa taaaacaagc
     2581 tgccctcgct gtggcacccg gccgcgatgg cccgcacccg gcggccgccc aggtcaaaga
     2641 cgtgcccgtc cccgatgggg agggcctggt agtcataatc gtaccgcacc tcggtggtgt
     2701 agcggttcgg gtccacccgc ttttgcccca gctcctccac ggcgccggcg gaggcgtaga
     2761 tcgggtactc tttaaactgc ccgttgcagg cggtgtggtc cccgtgggcg tgggtgttcg
     2821 ccacaatcag cggcaggtcg gtgatcgtgc gcacatagtc ccgcaggttc cgcacatgct
     2881 cgcccgggtc gatgaccagg gcggtgtggc cgccgatcag cagataggtc acggcgctga
     2941 ccgggtggcc ggtgatgatc caggtatccg gccggatcag gcggctggcg aattcttttt
     3001 catggatgag gtcaactggc attttggttc tcctttctct tttccgcgcg gcgcgggtca
     3061 aagctccgac aaaagttttt tctttttcca cctgccggtg atgaaataga aaaagctgat
     3121 cagaccgggc accagcatgc agaacgccgc gccccagaaa tagctctgca cgccctgtcc
     3181 gaacacatgg aaaaacagca ggcagaccag gatgcggcag acgccgtcca ggatgcccag
     3241 cagaaaacag agcccggcgg cgcccgcgcc ggtggcgatg gatttaaagc agctggcaaa
     3301 ggccaccacc agcatgccca ccgacatgat ccgcagaaag accacgccga actcgatcac
     3361 ctgcgcctcg ttggtgaaca ggcggaacag ctgccgcgga aagagcagaa agaccgcgca
     3421 gaccacaaag gccatcgaaa aattgcaggc cagcgtgtac cacatcgtct ttttgacccg
     3481 gtcctgccgc tgggcgccca ggttctggcc gatcatggcc ccggcggcgc cgtccacccc
     3541 catgacgaag acgttcataa acttttcgat cttgttgccg atgctgtagg tggaggaggg
     3601 caccaggccg tagacgttga tgttggcctt gacccagagc atcgagccct gcacggcgaa
     3661 cgagcgcgcc agctgcggca cccccatctt caaaatcagc ttcagcgggg tggcccggat
     3721 gcggaaaaag gaccagccca actgaaagtc gaacgcctcc cggtggcgat acatataaaa
     3781 gaacgccgca aaaaaggccc ccgcctggga cagcaccgtg gcgatggccg tgcccgcggc
     3841 ctgcagcggg aagaccgcca ccagcagcag gtctgcaaaa atattgatga ccgccgccac
     3901 ggtgataaaa atcagcggcc gggtggactc gcccatgccc cgcaggatcg aggccaccgc
     3961 gttgtacccg aagacgaacg gcatgcccag ggcggtgatc atcatgtact gcgccgcctg
     4021 cccccgggcc tccccggggc agttgagcat ctccaggatg gggcggtgaa acagcagcgc
     4081 cgccgccatc accaccaccg aggcgcccat gagcagggtc aacagggtgc ccacggcctc
     4141 cttgaggcgc ttttcctcct tggcgcccgc cagctgggcg atatagatct ggccgcccat
     4201 ggcgatggcc accgcaaaga tggtcataaa atcggccatc tcgccgcccg tcgccacccc
     4261 cacggtgccc acattgccca caaatttgcc gatcacgatc agatccacgg tggagtaaag
     4321 ctgctgcacc aggttcgtca gcaccatcgg cgccgcaaac aggatcagca gccggaagat
     4381 gggcccccgc gtcaggtcca gccccaccct ttttcgatcc gtcgtcaaac gcatcacatc
     4441 ccatcacaaa ataatggggc gcggggccgg gagatccctg ccctgcgccc cggtgcgggc
     4501 ggcgcggccg tccgccgcat cacccgcgcc ccgcgctcac ccctcgggcg ggtaaacgcc
     4561 gttcgccgaa taggcgatgg tgccgaagcc cttgacgaac ttgtgcatgc gcaccctctt
     4621 ttcgcccccc tggccgccgg cgtgctcgac cttgtagtcg tagcattccg ggtcggcgat
     4681 gatctggttc agcgcttcca ggatattgag cagcaggttg ttttccaggt tgaccacaaa
     4741 gtggccggga aaaatgtaat cgaactcgtc cagccgccgg cagagctttg ccagctcgtc
     4801 ccggtaggtg gacaggttgc agtactggtt gttcacgccc ccgggcctcg ggccgccgcc
     4861 gcagccgatc acatcgctgc acacgtcgtc gcccgcaaac aggcagcgtc cctttttgtc
     4921 caaaaacatc gcgtgcccgg cggcgtggcc gccggtccag accagctcca cctcatagcc
     4981 gccgcccagg tcccaagtgg tcccgttcgg cacccccacc agctcgtagt cgcggtactc
     5041 gggcaaatcc ttgcggtcaa actccagcca gatgttgttg ccgttctcat cgaacagata
     5101 atcccacgcg cccggcttgc acttgttgcg gatattctgc tcctcaatgt cgcagcagta
     5161 caccgggtca aagcgcacgt tgcccagcac atggtccggg ccggtgtggg tgttgaccac
     5221 gatcagcggc atgccgccgg tgatctcgtc caccaggcct ttcagatccc ccaggccgta
     5281 ggcggtatcc accagcatcg ccttttcggg gcccaggatc aggtacatcc acacatcccc
     5341 ggcgccgtcg cagttctggt tgaacaggcc gtacatattg tcgcggaacc ggtagacctc
     5401 cacatagggg tttacatcgt agacccgcat ggcgccgttt ttctcgcgca gctgtttcat
     5461 ataggcccag ctgatctggt tttggggcga gccccacttg agcctttttg ccctggaagc
     5521 gcccgctatg ggccacatct gtcccgtcat ccctccgccg tcctttccgc gcggccgcgc
     5581 cgcgctctgc cgtccgctca cttctccgtc ggcttgtagc cgtaaccgat gacgctgaag
     5641 cccttgacga atttgaagaa ccgctcgttc ttggccgtgc ggtccttgcc ccagctctcg
     5701 atcttgtagt cgcagttcgc cgggtcggcc aggatgctgt ccagcgcctc caggatgttg
     5761 ggcatgaggt tgttttccaa attgagcatg aagtgctgcg ggaagatata gtcgtactcg
     5821 cccatgcggt ccaccagcag cttgacccgg tcgcggtaaa aggacagctg cgtgcactcc
     5881 tggtgggggc cgttgccccg ggtgacgttg accgagccgc agccggacac gtcgctgcag
     5941 atattgtcgc cggtgaacag gatgcggttg tgcttgtcga ggtaggccgc atggcccgcc
     6001 gcatggccgc cggtgaagat cagctccacc tcatagccgc cgcccaggtc ccaggtatac
     6061 ccgtccggca cgccgatgat ctcatactcc tcgaacaccg gcaggtcctt gcggtcaaag
     6121 tccagccaga tgttgttgcc gaacgcgtcg aacaggtagt cccacatgtg ggcgtgctgg
     6181 gtctgcagat agggcaccag atactcgtgg cagtacgccc tgccgaaacg gcagttgccg
     6241 taggcgtggt cgaagtggtc atgggtattg accacgatga tctccttgcc gccggtgatc
     6301 tcgtccacca ggcccttcag gtcgcccagg ccgtagccgg tgtcgatgag catcgccttt
     6361 tccggcccca cgatcaggtg catccagacg tcgcccatgc cgtcgcagtt ggggtggaac
     6421 aggctgtaca ggttgtcgcg gaactggtag acctcgatat aggggtccac atcgtagacc
     6481 ctgcgggtct tgtcatgctc ccgcagctct ttcatgtagg cccagctgat ctggttctgc
     6541 ggcgcgtccc acttgatccg cttcgccttg ctcaggccct cgatgggcca catctgacct
     6601 ctcattttca ttacctccgt ctatgatgat ttaatcgaaa acaatggatt gccgccccac
     6661 ccgcatccgc tgcgggcggg ccgggcggcg ggtccgccgt ccgggcgcgg gccacaaaca
     6721 gggcggcgcg cgacccgccg cgggaaattg caattccccc ggcgccatgc taaactggtt
     6781 gcaaggggcg cgcccggcgc cggaaagaaa ggggaacggt atggatctcg tcgcttttca
     6841 atattttctc tcggtctccc agtgcctgag cttttccgag gccgcggagc agaaccacat
     6901 ctcccagtcc tcgctgtcca aggcgatcct gcgcctggag cgggagctga acgtcaagct
     6961 gttcgaccgc aaccaccacc ccatctcgct caccgcggcg gggctctgcc tgcaggagca
     7021 cctggggcag atgcggcctt tgtatgtaca ggccatgcgg gagctggacc gctaccgcgt
     7081 gcaccggaat atccgctgtt actttgtgcc ccgcctctac gcgctgaaaa acgcggcgct
     7141 ggcttttctg tccgccaacc gggacatcga gttgacgctg gagaccggct ccgacttcac
     7201 caccgccgtg cgggagatga tggaggggga ttacgacatg gcggtcatgc accagccctt
     7261 tgacctgccc gagggggtgc gcatgtcgat gatctacgac gacaagctgt atgccgtgct
     7321 gccccaaaac cacccgctgg cgggccgcga ggccgtctcg ctggccgagc tggacggcta
     7381 cacctttgtg gagaccaatt tttccgccac catcgtgcag ggcctggcgg ccaaattcgg
     7441 ctttcgcccc ggcggcatcg acgtgcaccg cagcatcacc ggcagccgcg aggaggtgat
     7501 gctgcgcatc gcccgcagcc agaacatcgg catcttctgc agccgtgaca tcacgctgta
     7561 caacatccag ccgctgaagc tggtcacccg gccgctgaaa gagatctcgt cactgcccat
     7621 cgtgctgctg gagcgggagg gccgcaccga caccgagtgg catcagcgct tttgcgcctt
     7681 tttgaaaaaa gaacaggaga actacgccgc gccgctctcc gccctcccct cggg
//
LOCUS       DBMDYA010000033         7680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2502_length_7680_cov_1.630426, whole genome shotgun sequence.
ACCESSION   DBMDYA010000033 DBMDYA010000000
VERSION     DBMDYA010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_7680_cov_1.630426"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="ACZPFK_01495"
     CDS             <1..1242
                     /locus_tag="ACZPFK_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HIR1405013.1"
                     /translation="DAITIPEFFSARFKEKKKVLLTISAVFILVFFTVYASSCFVTCG
                     KLFSTLFGLSYHSMMIAGALFVIFYTVIGGFLAESASDFMQSIVMIFALGVILIAGTA
                     AAGGPAAVAQNIRDIPGFTSFFGIAQPVVENGVQQVSGGAPLFGAAGSYGLLTILSTL
                     SWGLGYFGVPQVLLRFMAIRKTSELKKSRRIATVWCVLSLTSAVLIGMIGRALYPTAL
                     LTSSEAENIFVVLSTNLLPPLLAGVVMAGILAATISSSDSYLLIAASAFAKNIYQGVL
                     RRDASDKKVLWVSRIMLLAISAVGIVIALDENSVIFTLVAFAWAGFGATFGPIMLLSL
                     FWKRTTWRGAVAGMVSGAATVFVWKLFLKPLGGVFGIYELLPAFLVSLAFIVGVSLLD
                     KAPSAEIEAEFEEAKRMAAEA"
     gene            complement(1758..2222)
                     /locus_tag="ACZPFK_01500"
     CDS             complement(1758..2222)
                     /locus_tag="ACZPFK_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1405014.1"
                     /translation="MKISTKGRYALRLMVDLAVHTEDGGYAPIRSIAERQELSDKYLE
                     QLIAQLSRAGFVRSARGAQGGYRLSRDPATYTVGDILRLMEGDLAPVACGGNSASDPC
                     CGRYDNCATVEVWQQLSAAINQVVDGITLADLVRRYHEKCPSGACEPPIFGG"
     gene            complement(2259..3005)
                     /locus_tag="ACZPFK_01505"
     CDS             complement(2259..3005)
                     /locus_tag="ACZPFK_01505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1405015.1"
                     /translation="MRPVIGITANYTDDGRPFRELGIGAPGQRWDLLPEDYACAVERA
                     GGLPVILPLTAPETALELAGRMDGIVFSGGSDLAPALYGERAGEGANPPQEARDVHEL
                     PLLRELLKGSLPLLCICRGCQLLNVALGGTLHQDLVRAGLPAHSLAGHSLCQAAHRVR
                     VRPGTLLERLVGSDEIPVNSFHHQAVKAPASGVQVAAVDAGCEEVVEAIELPDRPAFT
                     LGVQWHPEALVDSEPSSLALWQGFLQAAGR"
     gene            3734..4633
                     /locus_tag="ACZPFK_01510"
     CDS             3734..4633
                     /locus_tag="ACZPFK_01510"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1405016.1"
                     /translation="MAAQRDGQNRRQVYRAAAPGPYGGPAHAGAGRGARPPARPAAPR
                     RPGRRRKRRGRGRRVVLTLFAAGLLCLLGGLALRSLLPRHYVVALDAGHGGSDPGASG
                     VIGEVALTETTVRYLEELLTADENYTPVLCRQYGEGKDVNARAAAARWHGADLLLSVH
                     GNSEESGQARGFECYPLPPGRTHHEDSLRLSGLLVDEMSAAGAEVRGENGIKYAYYFS
                     DGSGGYEKVIRESSDRTVYSEKSFGVLEGAGCPAVLAEQCFVTNAADAEAFGSEAGCH
                     LAAERYYRAICRYFGTQPTALEG"
     gene            5194..5700
                     /locus_tag="ACZPFK_01515"
     CDS             5194..5700
                     /locus_tag="ACZPFK_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1405017.1"
                     /translation="MAFIQIEPKEIADNPFKLIGSDWMLITAGDEKKCNTMTASWGGV
                     GVLWNKPVSFIFVRPQRYTREFIEREQAYSLCFFEDGFRPQLSYLGTASGRDEDKIDK
                     AGLTTAFEGGVPYFEQARLVLICKKSYRDDLKADCFLDAEADARCYPEKDYHRMYVGE
                     IVKVLKKK"
     gene            5749..6528
                     /locus_tag="ACZPFK_01520"
     CDS             5749..6528
                     /locus_tag="ACZPFK_01520"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1405018.1"
                     /translation="MVLTVDIGNSNIVLGGYEDGELSFFTRFATERKLEADQFAVEIR
                     GLLHLYAVDKSRITDVVISSVVPGITSLVAGALRRIVPVRALMLTTGGNTGVEIDIDN
                     PAELGADILSSAIAAKARYPMPCVIIDMGTATTLTAMNARGAVCGVAIMPGVYISQDA
                     LKMRTSQLHGISLEAPPHAIGRNTADSMKSGAVYGNASMLDGMIDRFAEELGGVATVV
                     ATGGAARKIVACCRHEIIYEPDLLLDGLYHYYLRNRGEQGE"
     gene            6854..7327
                     /locus_tag="ACZPFK_01525"
     CDS             6854..7327
                     /locus_tag="ACZPFK_01525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1405019.1"
                     /translation="MVVITMENGKQIKLELDAAAAPKTVENFLKLVKEGFYDGLGFHR
                     IIPGFMIQGGCPLGTGTGGPGYGIVGEFASNGHPNPIKHQRGVISMARSANPNSAGSQ
                     FFIMHADAPHLDGDYAAFGRVVEGMDVVDEIAAVPTGWSDKPEKPQIMKSVKVVD"
     gene            7328..>7680
                     /locus_tag="ACZPFK_01530"
     CDS             7328..>7680
                     /locus_tag="ACZPFK_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016060.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1405020.1"
                     /translation="MFKKLFSFFWDRSLLFFLIIGVLNTLVSMVGSQLLLGALGYWGS
                     TALMFAVCSVFSFIFNRKYSFQSKAPLLQSAVRFTVVIAVCYLVSFGLSDFLVPWVVD
                     AFAWPISTEWVTRIA"
BASE COUNT         1321 a         2444 c         2521 g         1394 t
ORIGIN      
        1 gacgccatca ccatcccgga gtttttctcc gcccgcttca aggagaagaa aaaagtgctg
       61 ctgaccatct cggcggtctt catcttggtc tttttcaccg tctatgcgtc cagctgcttt
      121 gtcacctgtg gcaagctgtt ctcgaccctg ttcgggctgt cctatcacag tatgatgatc
      181 gccggtgcgc tgttcgttat tttttacacg gtcatcggcg gctttctcgc agagagcgcc
      241 tcggacttta tgcagagcat cgtgatgatc tttgccctgg gggtgatcct gatcgccggc
      301 accgctgccg ccggcggccc cgcggcggtg gcgcagaata tccgggacat ccccggcttc
      361 accagctttt tcggcatcgc gcagccggtg gtggaaaacg gcgtgcagca ggtgtcgggc
      421 ggcgcgcccc tcttcggcgc ggcgggcagc tatgggctgc tcaccattct gtccaccctc
      481 tcctgggggc tgggctattt cggggtgccg caggtgctgc tgcgctttat ggccatccgc
      541 aagacctctg agctgaaaaa gtcccgccgc atcgccacgg tctggtgcgt gctctccctt
      601 accagcgcgg tgctcatcgg catgatcggc cgcgcgctgt atcccaccgc gctgctgacc
      661 tcgtcggagg cggaaaacat ttttgtggtg ctctccacca acctgttgcc gccgctgctg
      721 gcgggtgtgg tgatggcggg catcctggcc gcgaccatca gctcctccga ctcctacctg
      781 ctcatcgcgg cctcggcctt tgccaaaaat atctaccagg gcgtgttgcg ccgggacgcc
      841 agcgacaaaa aagtgctctg ggtctcccgc attatgctgc tggccatctc ggcggtgggc
      901 atcgtcatcg cactggatga aaacagcgtc atctttacgt tggtggcctt tgcctgggcg
      961 ggcttcggcg ccacgttcgg ccccatcatg ctgctgagcc tattctggaa gcgcaccacc
     1021 tggcgcggcg cggtggccgg catggtgagc ggcgcggcga ccgtctttgt ctggaagctg
     1081 ttcctcaagc cgctgggcgg cgtgttcggc atctatgagc tgctgcccgc ctttttggtg
     1141 agcctggcct ttatcgtggg ggtctccctg ctggacaagg cgccctcggc cgagatcgag
     1201 gcggagtttg aagaggcaaa gcggatggcc gcggaggcgt gagacggagg ccgcccgttt
     1261 tgcacgggca cagcgcggct ttcacaaaaa caaaggatca agcaaaaggg ccgggacggc
     1321 cgctcctctc aacagagggc ggctgtcccg gccttttttg cgcacggaaa aacgtacgga
     1381 aaatgaaaaa agccccggct ttttgggaga tggcgcgcag cgcggggaag attaaaaaac
     1441 aggcgttccg cttcagcact ggttcagcgg agagcgcagg acgcccgtct gattgcggcg
     1501 gacgggcccg cccgctgcgt cggcaacgct tgcaggtcct acggggccgc aagcgccact
     1561 gctctgcaat cgaacatttt tcgccagccg ccggtattgc gcagccttgc tccgtcgggc
     1621 aaaacaagct tttcctgacg gcacggtgct tttaaacggg gacgctcgct ttctgcgcgt
     1681 cggcccgctc gcgcccggcg gtgcgcaccc ggcaaaaggg cgcaaagcct cacggggcct
     1741 tgcggctcgg cggggattca gccgccgaag atcgggggct cacaggcgcc gctggggcat
     1801 ttttcatgat agcgccgcac caggtcggcc agggtgatgc cgtccaccac ctgattgatg
     1861 gcagccgaga gctgctgcca gacctccacg gtggcgcagt tatcgtagcg gccgcagcag
     1921 gggtcgctgg ctgagttgcc cccgcaggcc accggcgcaa ggtcgccctc catcaggcgc
     1981 aggatatccc ccaccgtgta ggtcgccggg tcgcgggaga ggcggtaccc cccctgtgcg
     2041 ccgcgcgcgc tgcgcacaaa gccggcgcgg gagagctgcg cgatcagctg ctccagatat
     2101 ttgtcgctga gctcctgtcg ctcggcaatg ctgcggatgg gggcatagcc gccgtcttca
     2161 gtgtggacgg ctaggtccac catcagccgc agcgcatagc gccctttggt cgagattttc
     2221 atgatatatg ctcctttgta agggggataa aagcggggtc agcgcccggc ggcctgcaaa
     2281 aagccctgcc agagggcgag gctggagggc tcgctgtcca ccagcgcctc cgggtgccac
     2341 tgcaccccca gcgtgaacgc cgggcgatcg ggcagctcaa tggcttccac cacctcctcg
     2401 cagcccgcgt cgacggcggc gacctgcaca ccggacgccg gggccttgac cgcctgatgg
     2461 tgaaaagagt tgaccggtat ctcgtcgctg cccaccagcc gctccagcag ggtgccgggc
     2521 cggacccgca cacggtgggc cgcctggcac agggagtgcc cggccaggga gtgggcgggt
     2581 aggccggccc gcaccaggtc ctgatgcagg gtgccgccca gcgccacgtt gagcagctgg
     2641 cagccacggc agatacacag cagcggcaga ctgcctttca gcagctcgcg cagcagcggc
     2701 agctcgtgga cgtcccgcgc ctcctgtggg ggattggccc cctccccggc gcgctcgccg
     2761 taaagggcgg gggcaagatc gctgccgccg gaaaagacga tgccgtccat ccgcccggcc
     2821 agttccagcg ccgtctcagg ggcggtgagg ggcaggatca ccggcaaacc gcctgcccgc
     2881 tcgaccgcgc aggcatagtc ctccggcagc aggtcccagc gctggccggg ggcgccgatc
     2941 cccaattcgc ggaaaggccg tccgtcgtcg gtgtaattgg cagtgatgcc gatcacgggt
     3001 ctcatctgca atcctcctgt ccccgcagcg ggggtttgtt atttttgtgt cgatctcaag
     3061 acagctccat tctaccatat cccggtagga ttgtatagat tggggatgga cattccgacg
     3121 cttttttcac aaaaaaagcc cgcggtcagc gggctgcgaa agagcgcgtg cgaggcactg
     3181 tgaggaggga atcggagggg gagcgctgcc ggcggagaag tatcccccta tccgcttttc
     3241 cccgctccga ttcgttccca ggaaaaagag actgagccgg ccccacggtc acgaccgcac
     3301 ctttgtcccg gccttggccg ccgtcttgcc ggggaaaagc tgcggccccc cgcccctgcc
     3361 tccgctctgc ggctgtgcgg gcccacagag atggaaaaag gagtggggcc cggttgcggc
     3421 catgggacga aagacatccc ccttgtgttt tcagcgaccc cgacggcccc gggccgttgg
     3481 aggggggccg acggcggggg cgggcgagcc cgtttttcgg ctgcctcgcc gcttcccaca
     3541 ccacttctct accccaacaa agtgaaattt ttcaataaaa attggagaaa attcaggttt
     3601 tattaagtat tgtgctgtag aatagaggct gtcgcgggct ggcggcggcg cgggcgcggg
     3661 aaactgccga ccggcgcagg gctgaggcgg cggttttgat tccatcacat cacataccac
     3721 agggggcggg cgcgtggctg cacagcggga cgggcaaaac agaagacagg tctaccgcgc
     3781 ggcggctccc gggccctatg gcggcccggc ccacgcgggc gcagggcgcg gcgcccgccc
     3841 tcccgcgcgt ccagccgcgc cccgacggcc gggcagacgc cgcaaaagac ggggccgcgg
     3901 gcggcgcgtc gtcctcaccc tgttcgccgc gggcctgctc tgcctgctgg gcggtctggc
     3961 gctgcgcagc ctgctgccgc gccactatgt ggtggcactg gacgcgggcc acggcggcag
     4021 cgacccgggg gccagcggcg tcatcggcga ggtggcgctc accgagacca cggtgcgcta
     4081 tctggaagag ctgttgacgg cagacgaaaa ctacacgccg gtgctctgcc gacagtatgg
     4141 tgagggcaag gacgtgaatg cccgcgccgc cgccgcccgc tggcacggcg cggacctgtt
     4201 gctgtcggtg cacggcaact cggaggagag tgggcaggcg cgggggttcg agtgctatcc
     4261 tttgccgccg ggccgcacgc accatgagga cagcctgcgc ctctcgggcc tgctggtgga
     4321 cgagatgtcc gccgccgggg ccgaggtgcg gggggagaat gggataaaat acgcctacta
     4381 tttttccgac ggcagcggcg gttatgaaaa ggtgatccgc gagtcgtccg accgcactgt
     4441 ctacagcgaa aagagctttg gggtgctgga gggcgcgggc tgcccggctg tgctggcgga
     4501 gcagtgcttc gtcaccaacg ccgccgacgc ggaggcattc ggcagcgagg cgggctgtca
     4561 cctggcggcc gagcgctatt accgcgccat ctgccgttat ttcggcactc aacccaccgc
     4621 gctggagggg tgacgtcgac tctcccgaca gggaacgacc gtcgccggat cttgacggaa
     4681 aaggcccgct ttctgtgtca gcgccgcttg tgggtcctac ggtcttgcgg cgttccgctt
     4741 ccttgcaacc gggcctcttc cgccgacaac cggatacttc acagttttgt cctgccgggc
     4801 aaaaccaagc gctcccgcat ggcgatgcat ttttcgtgcc ccggccgggg gccggtccct
     4861 ccggaccctt tcgcaggcat ggagaaaaga gataaggccg cttttggcct ttgcagagca
     4921 gaggcgatag agcgcggaag cggagccgag agggaaaagg ttcctcgcaa aggcgtcagg
     4981 cgccgctcac aggggagaac aaaaagggga gggagacggc ccaatgaggg ccgcctctct
     5041 ctttttcaca agctcccaca aaaaacaggc tactatttat ttcctgcttc gtgtataata
     5101 aggtatgcct gtttttgccc tttgccgcag gcaagggcgg gggacgggca cagatcacgc
     5161 gcccgtttta tgggtgcgat ggggggaatc agcttggctt ttatccagat tgaaccaaag
     5221 gaaatcgcag ataacccgtt caagctcatc ggcagcgatt ggatgctgat cacggcgggg
     5281 gacgagaaaa agtgcaacac catgaccgcc tcctggggcg gggtgggcgt tttgtggaac
     5341 aagccggtct cgttcatctt tgtgcggccg cagcgctaca cccgcgaatt catcgagcgg
     5401 gagcaggcct attcgctctg cttttttgag gacggctttc gcccccagct ctcgtatcta
     5461 ggcaccgcca gcgggcggga cgaggacaag atcgacaagg cgggcctgac caccgctttt
     5521 gagggcggcg tgccctattt tgagcaggcg cggctggtgc tgatctgcaa aaagagctac
     5581 cgcgacgacc tcaaagcgga ttgctttttg gatgcggagg ccgacgcccg ctgctacccg
     5641 gaaaaggact atcaccgcat gtatgtgggc gagatcgtca aggtgctgaa aaagaaatag
     5701 ggccgcgcgc ccgcgggcgc aaacgggccc caggatcggg gaaaacatat ggtactgaca
     5761 gtggacatcg gcaactccaa catcgtgctg ggcggctatg aggacggcga gctcagcttt
     5821 ttcacccgct tcgccaccga gcgcaagctg gaggcggacc agttcgcggt ggagatccgc
     5881 gggctgctcc acctctacgc ggtggacaag tcccgcatca ccgacgtggt gatctcgtcg
     5941 gtggtgccgg gcatcacctc gctggtggcc ggtgcgctgc ggcgcatcgt gccggtgcgg
     6001 gccctgatgc tgaccaccgg cggcaacacc ggggtcgaga tcgacatcga caaccccgcg
     6061 gagctgggcg cggacatcct gtccagcgcc atcgcggcca aggcgcgcta ccccatgccc
     6121 tgcgtcatca tcgacatggg caccgccacc accctcaccg ccatgaacgc ccgcggcgcg
     6181 gtgtgcggcg tggccatcat gccaggggtc tacatctcgc aggacgcgct gaagatgcgc
     6241 accagccagc tgcacggcat ctcgctggag gccccgcccc acgccatcgg ccgcaacacc
     6301 gccgacagca tgaagagcgg cgcggtctac ggcaacgcct cgatgctgga cggcatgatc
     6361 gaccgctttg cggaggagct gggcggcgtt gcgacggtgg ttgccaccgg cggcgcggcc
     6421 cgcaagattg tggcctgctg ccgccacgag atcatctacg agccggatct gctgctggac
     6481 ggcttgtatc actattacct gcgcaaccgg ggggagcagg gggaataggc tccacccgcg
     6541 gcccctccgg cgcggcctcg ccccaccgtc ccccccttgc gggggaagcg ggaacagcgc
     6601 gccgttggct ttgcgctctc acccaaggcg acgcactccg cggtaggcac ttttccctga
     6661 agcagcgcgc tttggggcat acctttgccc gcgcgggcct gcgcgctctt acccaaaaca
     6721 gcgcgcttcg cggctccccc ctgcaaagag aggcagaagc tgcgcgcctt tgtgccttat
     6781 gtttccccgc ggttttgccg gggcggcgca aaagctcccc ccggctcatc ataacagaaa
     6841 agaggacaag taaatggtcg tcatcacaat ggaaaacgga aaacagatca agctcgagct
     6901 ggacgccgcc gcggcgccca agacggtgga aaactttctc aagctggtga aagagggctt
     6961 ttacgacggg ctgggctttc accgcatcat cccgggcttc atgatccagg gcggctgtcc
     7021 gctgggcacc ggcaccggcg gcccggggta cggcatcgtg ggcgagttcg cctccaacgg
     7081 ccaccccaac cccatcaagc atcagcgcgg ggtcatctcg atggcccgca gcgccaaccc
     7141 caattcggcg ggcagccagt tcttcatcat gcacgccgac gcgccgcatc tggacggcga
     7201 ctacgccgct ttcgggcggg tggtggaggg catggacgtg gtggacgaga tcgccgccgt
     7261 gcccaccggc tggtcggaca agcccgaaaa gccccagatc atgaagagcg taaaggttgt
     7321 ggactaagtg ttcaaaaaac tgttttcttt cttttgggac cgcagcctgc tgttctttct
     7381 catcatcggc gtgctgaaca ccctggtctc gatggtgggc agccagctgc tgctgggcgc
     7441 gctgggctat tgggggtcca cggcgctgat gtttgcggtc tgcagcgtct tcagcttcat
     7501 cttcaaccgc aaatattcgt ttcagagcaa ggcccccctg ctgcagagcg ccgtccgctt
     7561 cacggtggtc atcgcggtct gctatctggt cagcttcggg ctgtccgact ttttggtgcc
     7621 ctgggtggtg gacgctttcg cctggcccat cagcaccgag tgggtcaccc gcatcgccat
//
LOCUS       DBMDYA010000034         7680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2503_length_7680_cov_1.455469, whole genome shotgun sequence.
ACCESSION   DBMDYA010000034 DBMDYA010000000
VERSION     DBMDYA010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_7680_cov_1.455469"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2577
                     /gene="secA"
                     /locus_tag="ACZPFK_01535"
     CDS             <1..2577
                     /gene="secA"
                     /locus_tag="ACZPFK_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1405021.1"
                     /translation="DILPDAFAACREASDRVLGMRHFPVQVIGGIVLHRGEIAEMKTG
                     EGKTLVATLPVYLNALAGNGVHVVTVNDYLAKRDSEWMGKVYRFLGLSVGLVTHDMPG
                     AERKRAYACDVTYGTNNELGFDYLRDNMVTYKEQMVQRGHAYAIVDEVDSILIDEART
                     PLIISGEGDKSTDMYKAADDFVVRLKKKVFADVDDKEEHDDEVEDDVDYIVVEKQKTV
                     TLTQNGIKRAEAHFHVENLADAENMTLSHHINQAIRAHGIMKRDVDYVVKDGEVIIVD
                     EFTGRLMLGRRFNEGLHQAIEAKEGVNVQKESKTLATITFQNYFRMYKKLAGMTGTAK
                     TEADEFNEIYALNVVEVPTNRPIVRQDMDDVVYKTERGKFNAVIEQIVESHAKGQPVL
                     VGTISIEKSELLSKMLKPHGIKHEVLNAKYHEKEAEIVAQAGKFGAVTIATNMAGRGT
                     DIMLGGNAEFMAKADLRRQGFDDELIAEADGHAETDDEEILKVRAAFGEALAKYKPIV
                     AEEAEKVKAAGGLFIIGTERHESRRIDNQLRGRSGRQGDPGATRFFLSLEDDLMRLFG
                     GERVQNLMDTLGVEEDMPIENRMITSTIESAQKKIEGQNFSIRKNVLSFDDVMNQQRE
                     IIYGQRNKVLQGDDVSESVRTMMEDSIKETVAQYTAGDASKEWDLSGLRDHYRGWLCR
                     DTDFQYSEEELASLQRDDVCDFLIQRARSVCESKERHYGSPMMRELERVVLLRVVDTK
                     WMEHIDNMDELRKGIYLRSYGQRDPVVEYRIEGFDMFDEMVASIREDTTRMLLTVEIR
                     TKEEPKREQVAIPVGEGVGGTKTNTKTPQKVKKIGRNDPCPCGSGLKWKKCTCKEYHP
                     DL"
     gene            2838..4520
                     /locus_tag="ACZPFK_01540"
     CDS             2838..4520
                     /locus_tag="ACZPFK_01540"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1405022.1"
                     /translation="MMRQPKWWQDAVVYQVYPRSFYDANGDGVGDLRGITEKLDYIAS
                     LGVDVVWSCPFFVSPWADGGYDIADYRAVDPAFGTMEDFEALVAGVHARGMKFVMDFV
                     GNHTSDEHPWFQAALADKDSPYHDYYTFRPGKDGREPSNWASIFGGSAWEYVPALDEY
                     YLHLFAKKQPDLNWENPKVVEEVLSILEFWADKGVDGYRLDAINYLYKEPGYPDVEPM
                     PGSPYGFATAHYANKPRVHEHFRTLRSRVMGPRGLSTVAEVAYLDDETARGYADPARG
                     ELDMLYLFDVLNVDQEGFDKFSPLPLDLPRFKRALAHWQQVLHGHGWLALFLGNHDQP
                     RAVSRFGDEGAYRARSAKMLANAMYFLQGTPYLYQGEELGMANPHYASLSQFKDVEAL
                     DALRAGLARGESEQHWLELLNARSRDCGRAPFSWDGGPAAGFTRGTPWMDPCADAEQV
                     NAAAEEADPDSVLHFYRALIRARKGRPSVLWGRTEFLAMDDPDLFLYTRTAEEEALFS
                     ANNFSGKERSFPLPEGFAGAEVLLSNCGAPQLDGDGVLTLRPWEGLTLLAKK"
     gene            4832..5095
                     /locus_tag="ACZPFK_01545"
     CDS             4832..5095
                     /locus_tag="ACZPFK_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HIR1405023.1"
                     /translation="MVESRCGLLCNECPFREQMNCKGCTQIDKPFWGESCPVKSCCEA
                     KSQEHCGQCKNFVCRLLHTFAYDMEQSDHGARIEHCKKWNEEE"
     gene            complement(5744..6718)
                     /gene="pta"
                     /locus_tag="ACZPFK_01550"
     CDS             complement(5744..6718)
                     /gene="pta"
                     /locus_tag="ACZPFK_01550"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR1405024.1"
                     /translation="MLEKLIETLKIKNKRLIYTEGCDPRILEAASRLHREGLLTPVLV
                     GEPGAVRAAAENAGFEIEGIEILDPLHYDGFEAMVDKMTELRKGKMTADECAAALKKA
                     NYFGTMYVKLGLADGLLGGATYSTADTVRPALQIVKTRPGSKIVSSCFIMDRTGADGK
                     PERYAMADCAINLHPSAEELVEIAVETAKTASFFGIDPKVALLSYSTLGSGKGEDVDK
                     VRAAAAQLKGMDLPFPVEGELQFDAAFAPEVGKLKAPGSKVAGEANVFVFPDINAGNI
                     GYKIAQRLGGFEAVGPILQGLAANINDLSRGCNAEEVYKMSIITAGME"
     gene            complement(6816..7385)
                     /locus_tag="ACZPFK_01555"
     CDS             complement(6816..7385)
                     /locus_tag="ACZPFK_01555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR1405025.1"
                     /translation="MMKKPTLREVFTIPNLLSYFRLLLIPLFVWLYLRAQSTRDFALA
                     AVVIGVSGFTDLFDGRLARRLGQITELGILLDPVADKLTEGAVIFCLATRYRWVVALV
                     VVYLLKEGFMTVAGLVMLRRGKKLDGAKWFGKVCTFVFYLVVFTLILWVEIPLGLANA
                     LIALCTGMMLFTFVMYARVYFYMGREKDC"
     gene            complement(7482..>7680)
                     /locus_tag="ACZPFK_01560"
     CDS             complement(7482..>7680)
                     /locus_tag="ACZPFK_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405026.1"
                     /translation="SAGADVHPLTALCADVHPLQPVTRMELFMWFSGATVPAKLTRGG
                     YFPPLFFMVESAVHKKLIKIS"
BASE COUNT         1569 a         2405 c         2394 g         1312 t
ORIGIN      
        1 gacattttgc ccgacgcttt cgccgcctgc cgcgaggcct ccgaccgggt gctgggcatg
       61 cgccacttcc ccgtgcaggt catcggcggc attgtgctgc accgcggcga gatcgccgag
      121 atgaagaccg gcgagggcaa aacgctggtg gcgacgctgc cggtctacct caacgccctc
      181 gccggaaacg gcgtgcacgt ggtcacggtc aacgactacc tggccaagcg cgactctgag
      241 tggatgggca aggtctaccg ctttttgggg ctgtcggtgg gcctggtgac ccacgacatg
      301 ccgggggccg agcgcaagcg cgcctacgcc tgcgacgtca cctacggcac caacaacgag
      361 ctgggcttcg actatctgcg ggacaatatg gtcacctata aagagcagat ggtgcagcgc
      421 ggccacgcct atgccatcgt ggacgaggtg gactccatcc tcatcgacga ggcccgcacc
      481 ccgctgatca tcagcggcga gggcgacaaa tccaccgaca tgtacaaggc cgccgatgat
      541 tttgttgtgc gccttaagaa aaaggtcttt gccgacgtgg acgacaagga ggagcacgac
      601 gacgaggtgg aggacgacgt ggactatatc gtcgtcgaaa agcagaagac cgtcaccctg
      661 acccagaacg gcatcaagcg ggctgaggcc cacttccacg tcgaaaacct ggcagacgcc
      721 gagaacatga ccctctcgca ccacatcaac caggccatcc gcgcccacgg catcatgaag
      781 cgggatgtgg actatgtggt caaggacggc gaggtcatca tcgtcgacga gttcaccggc
      841 cgcctgatgc tgggccgccg cttcaacgag gggctgcatc aggccatcga ggccaaagag
      901 ggcgtcaatg tccaaaaaga atccaagacg ctggcgacca tcacctttca gaactatttc
      961 cgcatgtaca aaaagctggc cggtatgacc ggtaccgcca aaaccgaggc cgacgagttc
     1021 aacgagatct acgccctcaa cgtggtggag gtgcccacca accggcccat cgtgcgccag
     1081 gacatggacg acgtggtcta caagaccgag cgcggcaaat tcaacgccgt catcgagcag
     1141 atcgtggaga gccacgccaa gggccagccg gtgctggtgg gcaccatcag catcgaaaag
     1201 agcgagctgc tctcaaaaat gctcaagccc cacggcatca agcacgaggt gctgaacgcc
     1261 aaatatcacg aaaaagaggc cgagatcgtg gcccaggccg gcaagttcgg cgcggtgacc
     1321 atcgccacca acatggccgg gcgcggcacc gatatcatgc tgggcggcaa cgccgagttc
     1381 atggccaagg ccgacctgcg ccggcagggc tttgacgacg agctgatcgc cgaggcggat
     1441 ggccacgccg agaccgacga cgaggagatt ctcaaggtgc gggcggcctt tggcgaggcg
     1501 ctggcaaagt acaagcccat cgtggccgag gaggccgaaa aggtcaaggc cgcgggcggc
     1561 ctgttcatca tcggcaccga gcggcacgag tcccgccgca tcgacaacca gctgcgcggc
     1621 cggtcgggcc gccagggcga cccgggtgcg acccgctttt tcctcagcct ggaggacgac
     1681 ctgatgcgcc tgttcggcgg cgagcgggtg cagaacctga tggacaccct cggcgtcgag
     1741 gaggatatgc ccatcgagaa ccgcatgatc accagcacca tcgagagcgc tcagaaaaag
     1801 atcgagggcc agaacttcag catccgcaaa aacgtcctct cctttgacga cgtcatgaac
     1861 cagcagcgcg agatcatcta cggccagcgc aacaaggtgc tgcagggcga cgacgtgtcc
     1921 gagagcgtgc gcaccatgat ggaggattcg atcaaggaga ccgtggccca gtataccgcg
     1981 ggcgacgcct ccaaggagtg ggacctctcg ggcctgcgcg accattaccg cggctggctg
     2041 tgccgcgaca ccgacttcca gtacagtgaa gaggagctcg cctctctgca gcgggacgac
     2101 gtgtgcgact ttttgatcca gcgggcccgc agcgtctgcg agagcaagga gcgccattac
     2161 ggcagcccga tgatgcgcga gctggagcgg gtggtgctgc tgcgggtcgt ggacaccaag
     2221 tggatggagc acatcgacaa catggatgag ctgcgcaagg gcatctacct gcgcagctac
     2281 ggccagcgcg acccggtggt ggagtaccgc atcgagggct tcgacatgtt cgacgagatg
     2341 gtggcctcca ttcgcgagga caccacccgc atgctgctca ccgtggagat ccgcaccaag
     2401 gaggagccca agcgcgagca ggtggccatc ccggtgggcg agggtgtggg cggcaccaag
     2461 accaacacca agaccccgca aaaggtcaaa aagatcggcc gcaacgaccc ctgtccctgc
     2521 ggcagcggtt taaaatggaa aaagtgcacc tgcaaggaat atcatcctga tctgtgacgg
     2581 ctgccccggc atcaacatcc tccccctctc cggcccgctt ttcgggcccg ccggagaggg
     2641 gtttgttttg cctcccgccg gttcgcggca aaggggcggg cagctgccgc gcggtcggcg
     2701 gcgcgggcat ggaaatgcgc gcgcgcctgt ggtaaaatca ttctataaaa cgcaaaatac
     2761 cgggcaggcc cctgcctgaa atcccggccg cgcgccgggg cgcaggaggg gcgggcccgc
     2821 aaaaaggaga aatgcaaatg atgcgacaac cgaaatggtg gcaggacgcg gtggtctacc
     2881 aggtctaccc gcgcagcttt tacgacgcca acggcgacgg ggtgggcgac ctgcgcggca
     2941 tcaccgaaaa gctggattac atcgcctcgc tgggggtgga cgtggtctgg agctgcccgt
     3001 tttttgtcag cccctgggcg gacgggggct atgacatcgc cgactaccgc gccgtcgacc
     3061 ccgctttcgg caccatggag gattttgaag cgctggtggc gggcgtccac gcccggggca
     3121 tgaagtttgt catggacttc gtcggcaacc acacctcgga cgagcacccc tggtttcagg
     3181 cggcgctggc ggacaaggac agcccctatc acgactacta cacgttccgc cccggcaagg
     3241 acgggcggga gcccagcaac tgggcgagca tcttcggcgg cagcgcctgg gagtatgtgc
     3301 cggcgctgga cgagtattat ctgcatctgt tcgccaaaaa gcagcccgac ctgaattggg
     3361 agaaccccaa ggtggtggaa gaggtgctct ccatcctgga attctgggcc gacaaagggg
     3421 tggacggcta ccggctggac gccatcaact atctgtataa agagccgggt tacccggatg
     3481 tggaaccgat gcccggctcc ccctacggct tcgccaccgc acactatgcc aacaagccca
     3541 gggtacacga gcactttcga accctgcgca gccgggtgat ggggccacgc ggcctttcca
     3601 ccgtggccga ggtggcctat ctggacgacg agactgcccg cggctacgca gacccggccc
     3661 gcggcgagct ggatatgctg tacctgttcg atgtgctcaa cgtggaccag gagggctttg
     3721 acaagttttc tcccctgccg ctcgacctgc cccgcttcaa gcgcgccctg gcccactggc
     3781 agcaggtgct gcacgggcac ggctggctgg cgctgttttt gggcaaccac gaccagccgc
     3841 gggcggtgag ccgcttcggc gacgaggggg cgtaccgggc ccgctcggca aagatgctcg
     3901 caaacgccat gtactttttg cagggcacgc cttatcttta ccagggcgag gagctgggca
     3961 tggccaaccc ccactacgcc tccctttccc agttcaagga tgtggaggca ctggacgcgc
     4021 tgcgggcggg tcttgcccgg ggtgagagcg agcagcactg gctggagctg ttgaacgccc
     4081 gctcccgtga ttgcggccgc gcgcctttct cctgggacgg cggcccggct gcgggcttca
     4141 cccgcggcac gccctggatg gacccctgcg ccgacgcgga gcaggtcaac gcggccgccg
     4201 aggaggcgga ccccgactca gtgctgcatt tttaccgggc gctgatccgt gcccgcaagg
     4261 gccggccctc ggtgctatgg gggcgcacgg agtttttggc catggacgac ccggaccttt
     4321 tcctctacac ccgcacggcg gaggaggagg cgctgttctc ggccaacaat ttctccggca
     4381 aagagcgctc tttccccctg ccggagggct ttgccggggc cgaggtgctg ctgtccaact
     4441 gcggcgcgcc gcagctggat ggcgacggcg tcctcaccct gcggccctgg gaggggctga
     4501 cgctgctggc aaaaaagtaa aggcagcact ggccctgcgg cgctttcggg cgccgcattt
     4561 ttttatcttc acagacccgt gtcccgccgc cccgctgcgg ctgaaagagg aaagtccaca
     4621 acaaccagcc cctgacccac acggctttga ggctttgtcc ggctgcagga cgtcggaggc
     4681 ccgtgccgcc ccgccgcctg aatccaaact gttgatggcc caactcaaag attgcttcaa
     4741 acgtcctacc cggttcccgc ccaaaaagtc aacatgacat atagatgtcc tgttggccgc
     4801 gctatactga aacaaaaagg aggggttgct tgtggtggaa tcaagatgcg gacttttatg
     4861 caatgaatgc ccttttcgag aacagatgaa ctgtaagggc tgcacgcaga tcgacaaacc
     4921 attttggggt gaaagctgcc cggtcaaatc ctgctgcgag gcgaaaagcc aggagcattg
     4981 cgggcaatgc aagaattttg tctgccggtt attacacacc tttgcctatg atatggagca
     5041 aagcgatcac ggtgcaagaa ttgaacactg caaaaagtgg aatgaggaag aatagcctcc
     5101 cgtaaaagaa agttggattt tatcctggag ttccctgggg ggagggcgaa gcgccgtcgc
     5161 cgataaagca ggggcggaaa aattcgcgtt tcgtccgctt gggcccgcag tggataaaag
     5221 ggccggtaac ccggcggctc cggggaatgc gccgccgggg aacggaaaaa atccgggccg
     5281 agcggaaagc ccagcgccaa agccccggac ggatcgcgcc tgccgccaca tggcacacgc
     5341 aggctttcac gctctgcttc gtcgggcggg ggaaaccacg caaaaaacgg tttgcgcctg
     5401 cctgctccag cggcggcagc ccccctcttc caagtcgcat cccgccgctt ccatccgcag
     5461 cgccgtcccg ggagacaact gtttcagcag aacctgacaa cggcccaggg cttttcagcg
     5521 cagcaaaggc aaaccacatt ttggacacgt tgcgctcaga tgcatccacc ctctatcggc
     5581 atatcgcggc aaacacagcg cgggccgtgc ctgagaagtt tgaccgggcg gagagaaacc
     5641 ggcccccacc ggcagggagg ggcgcgaaaa gagatccgga tgcggccgca cttgcggccg
     5701 catccggatc tctttttctg tgctcttttg cctttgacgc tttttattcc atgcctgccg
     5761 tgatgatgga catcttgtag acctcctcgg cattacagcc gcgggagagg tcgttgatgt
     5821 tggcggccag gccctgcagg atggggccca cggcctcaaa gccgcccagg cgctgagcga
     5881 tcttgtagcc gatgttgccc gcgttgatgt cggggaaaac aaagacgttg gcctcgcccg
     5941 ccactttcga gccgggggct ttcagcttgc ccacctcggg cgcaaaggcg gcgtcgaact
     6001 gcaattcgcc ctccaccggg aaaggcagat ccatgccctt gagctgggcg gcggcggcgc
     6061 gcaccttgtc cacgtcctcg cccttgcccg accccagcgt gctgtaggac agcagcgcca
     6121 ccttggggtc aatgccgaaa aagctggcag tcttggcggt ctcgacggcg atctccacca
     6181 gctcctccgc agaggggtgc aggttgatgg cgcagtcggc catggcgtag cgctccggct
     6241 tgccatccgc gccggtgcgg tccatgataa agcagctgga gacgatcttg ctgcccggcc
     6301 gggtcttgac gatctgcaag gccgggcgca cggtgtcggc ggtggagtag gtcgctccgc
     6361 ccaacaggcc gtcggcaagg cccagcttga cgtacatggt gccgaagtag ttggcctttt
     6421 tcagcgccgc cgcgcactcg tcggcggtca tcttgccctt gcgcagctcg gtcatcttgt
     6481 ccaccatggc ctcaaagccg tcgtagtgca gcgggtcgag gatctcgatg ccctcgatct
     6541 caaagcccgc gttctcggcg gcggcccgca ccgcgcccgg ctcccccacc agcaccgggg
     6601 tcagcagccc ctcgcggtgc aggcggctgg cggcctccaa aatgcggggg tcgcagccct
     6661 cggtgtaaat caggcgcttg tttttgatct tcaaggtctc gatcagtttt tccagcatac
     6721 tttttgtact cctttcgctc ccgccgccgc ggcgcgcggc ggcgctgtgt tccgatgttt
     6781 attgtaccac gttcgggcgc gcaaaacagc ctgtttcagc aatctttttc acgccccatg
     6841 taaaaatata cccgggcgta catgacaaag gtgaacagca tcatgccggt gcacagcgca
     6901 atcagggcgt tggcaaggcc cagcgggatc tccacccaga ggatgagggt gaagaccacc
     6961 agatagaaca cgaacgtgca caccttgccg aaccacttgg cgccgtccag ctttttgccc
     7021 cgccgcagca tcaccagccc ggccacggtc ataaagccct ctttcagcag atagacgacc
     7081 accagcgcca ccacccagcg gtagcgggtg gcaaggcaaa agatgaccgc gccctcggtg
     7141 agcttatccg ccaccgggtc cagcaggatg cccagctcgg tgatctgccc caggcggcgc
     7201 gccagccgcc cgtcgaacag gtcggtgaag ccggacacgc cgatcaccac cgccgccagg
     7261 gcgaaatcgc gggtgctctg cgcccgcaga taaagccaca caaaaagagg gatcaacagc
     7321 agccggaaat agctgagaag attggggatc gtgaagacct ccctcagggt cggctttttc
     7381 atcataggct tttgtcccct cccgcgccgc gtgcttgccg ccgcgccgct cacttttcca
     7441 ttcatacgag gcaggacgca aaatcgcctc aaattgatat tttacgatat ttttattaat
     7501 tttttgtgaa cggcgctttc caccataaaa aagaggggcg gaaagtatcc gcccctcgtc
     7561 agtttcgccg ggactgttgc cccggagaac cacatgaaaa gctccatccg tgttacaggt
     7621 tggagaggat gtacatcagc acaaagagca gtgagaggat gtacatcagc accggcactt
//
LOCUS       DBMDYA010000035         7669 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2506_length_7669_cov_1.320825, whole genome shotgun sequence.
ACCESSION   DBMDYA010000035 DBMDYA010000000
VERSION     DBMDYA010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_7669_cov_1.320825"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="ACZPFK_01565"
     CDS             complement(<1..222)
                     /locus_tag="ACZPFK_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405027.1"
                     /translation="MRSTPNNKMATKPVFPLLMSMALPPMLSMLLGSLYNIIDSMFVA
                     RYSADALTAVSLAFPLQNFALAVSVGAGVG"
     gene            complement(562..1434)
                     /locus_tag="ACZPFK_01570"
     CDS             complement(562..1434)
                     /locus_tag="ACZPFK_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013078.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1405028.1"
                     /translation="MPDAYAHIRTARHALALSGTDIPSDVAFRAGANGPDPFFVYRFW
                     DGSPAVDLAAFAQRMHAERAGPFLAALARLAATPAQQSYAAGFALHNALDSLAHPYVA
                     FLTAEGGPYDIPQGHCYYEAALDTALWRQDGGRGLPPLDLVAPPLITAELGEVAALLV
                     RAVGEVYGEELPFPALVDAFSHFRLARWFFRSRFGVKKLAARGVDAALGKPHYALSHT
                     QPRKLLPGVPEDWTDPFTGEEKRGGAASLLAAAEERGAGYLAALRRYWDGGLQLDGLA
                     RLLGSASYETGLPC"
     gene            complement(1563..2924)
                     /locus_tag="ACZPFK_01575"
     CDS             complement(1563..2924)
                     /locus_tag="ACZPFK_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405029.1"
                     /translation="MLKSFTKQEKSWMMYDWANSAHSVVVVTILPIFYNTVAGFTRDA
                     ASSMSVWGYATSIAMLIIALVAPILGVFGDFEGFRKKLFTIFMVVGVLACAGLAVTPL
                     TGFQNEAVAEKVGMAVLALYVVSQIGFAGANIYYDSFLTDVTTNERMDRVSTMGFGLG
                     YIGGSTIPLLAFLILNAVGVDMLWCLSFAFGFTAVWWFVFSYPLLKNVEQKSFVKREK
                     GAVRQAVRGLSKTMKDIFHYKTMFVFLLAYFFYIDGVNTIIHMSTSYGDTLGLSSTSM
                     LLALLLVQVLGLPFCLIYTKLAARFGAQFMVGVGICIYMGITVFGFFVRSEWQFWVLA
                     VLVATSQGGIQALSRSTFGKLIPDKNRSGEFFGFYDIFGKFSAIIGPSLVGLCSAIAA
                     NVIYRREGIDAATAPATLLEAVSQRAAPWGVLSILIIFLIGAALFFFVLPRYQKREAG
                     GSK"
     gene            complement(3123..3812)
                     /locus_tag="ACZPFK_01580"
     CDS             complement(3123..3812)
                     /locus_tag="ACZPFK_01580"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405030.1"
                     /translation="MQKKEIPAVETRALCKAYHTGDTALPVLREVALTIPAGCFAAIT
                     GPSGSGKSTLLSVLGCLDTADSGSLRLFGQEVAGADEKTLSALRGGTLGFVFQSFNLL
                     PGRTALENVELPLRYRRLPAARRRALAAQALASVGLADRAGHLPGQLSGGQQQRVAIA
                     RALAMRPRLLLADEPTGSLDSASAAGVLECMQQLLALGTTVVLVTHDLSLAARAPLRF
                     EMRDGVLSAVS"
     gene            complement(3917..4888)
                     /locus_tag="ACZPFK_01585"
     CDS             complement(3917..4888)
                     /locus_tag="ACZPFK_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405031.1"
                     /translation="MSESYENNGMPVDDGQPAAPGMGPSPDPMPRPEPSPAPEAAPQA
                     PRRVRRVGTLTMGVALILTGVAIGASLIWPSFDLTLIFRLCPLILVVLGCEVLVSSFA
                     KGDVKLKYDFLSMLVCFVLIVASLGVSLVPYALRYWGPDRDLVESRVERQLEEEVYRL
                     LKDDAAVSDCAANVYFEGYEFDKNATLETLDKGDHVGIRIDLRGPYDNARTFAEDCAR
                     VRDKLLAGGLEPSALNFEWNVSTDDYYAHANLHLSGAFQMKEDPENLAARVDSQLEIY
                     GNEEQEGYHGSLREGFPTAPAAPSAPAAPQAPSAPEAPSAPTVPDAA"
     gene            complement(4891..5442)
                     /locus_tag="ACZPFK_01590"
     CDS             complement(4891..5442)
                     /locus_tag="ACZPFK_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405032.1"
                     /translation="MKKHPIATFIFACWPGAGQMYLGYTKRGVSLMTAFMLTIGISGF
                     LNIGIFSILLPVIWFYAFFDTWSIRNMDQAQRIASPDDFIVPNEVRRRGEAEAFARRY
                     HTAFGVGFILVGIYVLYDNLLMPLLATLLNSISYDLSWLWRLAGNLPTLVVALIIILL
                     GIRLIRGDAKRDQDDVVEFRGEE"
     gene            complement(5439..5954)
                     /locus_tag="ACZPFK_01595"
     CDS             complement(5439..5954)
                     /locus_tag="ACZPFK_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1405033.1"
                     /translation="MDVFDDRTGHITAEGFAALLAGELDELASLEVAEHLSFCDSCCA
                     RYAGLLTGEVLLEPERPLAPPVMERVKRHGRVIFFRRAMKVGLAACLALTLWFSGVFT
                     LLPSAIGTAGPDRTAQRLEQEQRRNEAAEKELKEQLDTADAPEGTLNMRISNAIDQLF
                     GALNQKGESDK"
     gene            complement(5941..6486)
                     /locus_tag="ACZPFK_01600"
     CDS             complement(5941..6486)
                     /locus_tag="ACZPFK_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1405034.1"
                     /translation="MKGGEAVVTDEAFEQLVEKYQKLVYTVCYQMTKDPHTAEDLAQE
                     TFLSAYTHRDSCTPDTAKPWLCRIATNKAKDFLKSAYNRRVDLTDNDEDAQYNGSASP
                     PTEDLVVDAAMRREIEGLIRALKEPYLQVSVLYFLEQRSVDEIAARLGRPPKTVHTQL
                     YRAKQILQQRLIKGGVTRGRV"
     gene            complement(6672..7163)
                     /locus_tag="ACZPFK_01605"
     CDS             complement(6672..7163)
                     /locus_tag="ACZPFK_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1405035.1"
                     /translation="MLTLFKKYFWVFLVSMVPIVELRGAIPIGVASGLPLAGTYITAI
                     IGNMLPVPIIILFSKKVLLWCCRLPGAWGKPFNWIYSKGVKAGDKMQEKAGRGLYVAL
                     FLFVAIPLPGTGAWTGSLAATLLDMKFWPSVLAVMCGVLTAGVIMGALSAGLFGGLGM
                     IFA"
BASE COUNT         1415 a         2459 c         2542 g         1253 t
ORIGIN      
        1 gcccacgcct gcgcccaccg acacggccaa agcgaaattt tgcagcggaa aagcgaggct
       61 gacggcggtc agggcgtcgg cgctgtaccg cgccacgaac atgctgtcga tgatgttgta
      121 caggctgccc agcagcatcg agagcatggg cggcagcgcc atgctcatca gcagcgggaa
      181 gacgggcttt gtggccatct tgttgtttgg ggtggatcgc atagtggcaa gaccttcctt
      241 tcacggttcc gggtggttgc cgcggccccc gcccgcgccc ggcccgcaag cgggggatgc
      301 ggaaaagggg cgaaaaacgc ggcttttttc ttgaatgggt gcaaaaaagc acaaaaaaag
      361 accccaaccc gatctgtttc agttatccag ggaaacagaa aaagttggaa tcattccggc
      421 aggaccggcg aacattcaat ttttacctgc taagtatagc atctcggccg ggcgttgtaa
      481 agagggcggg gcaaagaaca ttttggcagc cccgcccaag gccgcagccg gggcgcggcg
      541 cggagcgttt ggctgggggg ttcagcaggg caggccggtc tcgtagctgg cgctgcccag
      601 cagccgggca aggccgtcca gctgcaggcc gccgtcccaa taccgccgca gcgccgccag
      661 atagccggcc ccgcgctcct ccgcggccgc cagcagggag gcggcgccgc cccgcttctc
      721 ctcgccggta aacgggtcgg tccaatcctc cggcacaccg ggcagcagct tgcgcggctg
      781 ggtgtggctg agggcgtagt ggggcttgcc cagcgccgcg tccaccccgc gggccgccag
      841 ttttttcacc ccgaagcggg agcggaagaa ccagcgcgcc aggcgaaaat gcgagaacgc
      901 gtccaccagg gccgggaaag ggagctcctc gccgtagacc tcccccacgg cgcgcaccag
      961 cagcgccgcc acctccccca gctccgccgt gatcaggggc ggggccacca ggtcgagcgg
     1021 cggcaggccg cgcccgccgt cctgccgcca aagcgcggtg tccagcgccg cctcatagta
     1081 gcagtgcccc tgtgggatgt cgtaggggcc gccctcggcg gtgagaaagg ccacataggg
     1141 gtgggccagg ctgtccagcg cgttgtgcag cgcaaagccc gccgcatagc tctgctgggc
     1201 gggggtggcc gccagccgcg ccagagcggc caggaaaggc cccgcccgct cggcgtgcat
     1261 gcgctgggca aaggcggcca ggtcgacggc cgggctgccg tcccaaaagc ggtagacgaa
     1321 aaacgggtcg ggcccgttgg ccccggcgcg gaaagccacg tcggagggga tatccgtccc
     1381 cgacagggcc agcgcgtgcc gggcggtgcg gatatgtgcg taagcgtcgg gcatgaaaaa
     1441 acctccaaaa gccgggcggc ggcgccctgc cgccgcaaaa gaccgggccg ccgcaggcgg
     1501 tccggtctcc ccggtccaat ttgctgaaat gggcgggcgg cgttcagcgc cctgcgcggg
     1561 ctttacttgc tcccgccggc ctcccgcttc tgatagcggg gcagcacgaa gaaaaagagc
     1621 gcggcgccga tcaaaaagat gatcaggatg ctcagcaccc cccagggcgc ggcccgctgg
     1681 ctgacggcct ccagcagggt ggcgggcgcg gtggccgcgt cgatgccctc gcggcgatag
     1741 atcacgttgg cggcgatggc ggagcacagg cccaccagcg acggcccgat gatggctgag
     1801 aatttgccga agatatcgta aaagccgaaa aattcgcccg agcggttctt atctgggatg
     1861 agcttgccga acgtgctgcg ggagagcgcc tggatgccgc cctggctggt ggccaccagc
     1921 accgccagca cccagaactg ccactcgctg cgcacaaaaa agccgaatac cgtgatgccc
     1981 atatagatgc agatgcccac gccgaccatg aactgggcgc cgaaccgggc cgccagcttg
     2041 gtgtagatga ggcagaaagg cagccccagc acctgtacca gcagcagggc cagcagcatg
     2101 gaggtggagc tgagccccag cgtgtcgccg taactggtgg acatgtggat gatggtgttg
     2161 acgccgtcga tgtaaaagaa gtaggccagc aaaaagacga acatcgtctt gtagtggaag
     2221 atgtctttca tcgttttgga gaggccgcgc accgcctgac gcaccgcgcc cttctcccgc
     2281 ttgacaaagc ttttctgctc cacgtttttg agcagcggat agctgaacac gaaccaccag
     2341 acggccgtga agccgaacgc gaaagagagg caccagagca tatcgacacc cacggcgttc
     2401 aggatcaaaa aggccagcag cgggatggtg gagcccccga tatagcccag gccgaagccc
     2461 atggtggaga cccggtccat ccgctcgttg gtggtcacgt cggtcaaaaa gctgtcgtaa
     2521 tagatgttgg cgcccgcaaa gccgatctgg ctgaccacat acagcgccag cacggccatg
     2581 cccacctttt cggccacggc ctcgttttga aacccggtga gcggggtgac ggcgagcccg
     2641 gcgcaggcca gcacccccac caccataaag atggtgaaca gctttttgcg aaagccctca
     2701 aagtcgccga atacccccag gatgggcgcg accagcgcga tgatcagcat ggcgatgctg
     2761 gtggcgtagc cccacacgct catgctggag gcggcgtccc gcgtaaaacc ggccacggtg
     2821 ttgtaaaaga tgggcaggat ggtgaccacg accaccgagt gggcgctgtt ggcccagtcg
     2881 tacatcatcc agcttttttc ctgcttggta aagcttttca gcaaatgatt caccctcgtt
     2941 tccggccgcg ggcaagggcc cggggcgaca gtgtggcgga gaacgcaccg cggcgcgtct
     3001 tgcagcctgc cctcattgta gcatgtcgaa aaatatgccg caatgagttg accgtttttt
     3061 tacagaaagg cgaaaaacgc ggcgtttctt tggcggatat gccggaggat gggggcgggg
     3121 cctcagctca cggccgagag aaccccgtcc cgcatctcaa agcgcagcgg cgcccgggcg
     3181 gcgagggaga ggtcgtgggt gaccagcacc actgtggtgc ccagggccag cagctgctgc
     3241 atgcactcca gcaccccggc ggcggaggcg ctgtccaggc tgccggtggg ctcgtccgcc
     3301 agcagcagcc tgggccgcat cgccagcgcc cgggcaatgg ccacccgctg ctgctggccg
     3361 ccggagagct gccccggcag atggccggcc cggtcggcca ggcccaccga cgccaaggcc
     3421 tgggcggcca gggcgcgccg ccgggcggcg ggcaggcggc ggtagcgcag cggcagctcc
     3481 acattctcca gcgccgtgcg gccgggcagc aggttgaagc tctggaacac gaaccccagg
     3541 gtgccgccgc gcagcgccga gagcgtcttt tcatccgccc ccgccacctc ctgcccaaac
     3601 agccgcaagc tgccggaatc cgccgtatcc aggcacccca gcaccgacag cagggtggat
     3661 ttgccgctgc ccgagggccc ggtgatggcg gcaaagcagc cggcggggat ggtgagcgcc
     3721 acctcccgca gcaccggcag ggccgtgtcc cccgtgtgat aggccttgca cagcgcccgc
     3781 gtctccaccg cgggtatttc ctttttctgc atcaaaaatc cccctccccg aacgcccgcc
     3841 cgcaatttga gcgtgcagcc tttagtgtgt tcggaaaggg ggattttatt taaaaggact
     3901 tgttgccaaa aggggatcag gccgcgtccg gcacggtggg ggcggagggg gcctccggtg
     3961 cggagggcgc ttggggagcg gcgggcgccg agggggccgc cggcgcggtg gggaagccct
     4021 cgcgcagaga gccgtgatag ccctcctgct cctcgttgcc gtagatctcc agctgagagt
     4081 ccacccgcgc cgccaggttc tccgggtctt ctttcatctg gaaagcgccc gagagatgca
     4141 ggttggcgtg agcgtagtag tcgtcggtgc tcacattcca ttcgaaattg agggcagacg
     4201 gctccaggcc gccggcgagc agcttgtcgc ggacgcgggc gcaatcctcg gcaaaggtcc
     4261 gcgcgttgtc ataggggccg cgcaggtcga tgcggatgcc cacgtggtcg cccttgtcca
     4321 gggtttccag cgtggcgttt ttgtcgaact cgtagccctc aaaatagacg ttggcggcgc
     4381 agtcggacac cgcggcgtcg tctttcagca ggcgatagac ctcctcctcc agctggcgct
     4441 ctacccggct ctcgacaaga tcgcggtccg ggccccagta gcgcagggca taaggcacca
     4501 gcgagacgcc cagtgaggcc acaatcagca cgaagcagac cagcatggag agaaaatcat
     4561 atttcagctt gacatcgccc ttggcaaagc tgctcaccag cacctcgcag cccagcacca
     4621 ccaaaatgag ggggcacagg cggaagatca gcgtcaggtc aaaggagggc cagatcaggg
     4681 aggcaccgat ggcgacgccg gtgaggatca gcgccacgcc catcgtcagg gtgcccaccc
     4741 ggcgcacccg acgcggcgcc tgaggcgcgg cctccggggc gggggagggc tcgggccggg
     4801 gcatggggtc cgggctcggg cccatgccgg gggcggcggg ctggccgtcg tccacgggca
     4861 tgccgttgtt ctcgtaggat tcactcatga ttattcctcc cctctgaatt cgaccacatc
     4921 gtcctggtcg cgctttgcgt cgccgcggat gagccgtatg cccagcagga tgatgatcag
     4981 ggccaccacc agcgtgggca ggttgcccgc cagcctccac agccaggaca ggtcgtagga
     5041 gatggagttg aggagggtgg ccagcagcgg catcagcagg ttgtcgtaca gcacgtagat
     5101 gcccaccagg ataaagccca cgccgaaagc ggtgtgataa cggcgggcaa aggcctcggc
     5161 ctcgccgcgg cggcgcacct cattggggac gataaagtcg tcgggcgacg cgatgcgctg
     5221 ggcctggtcc atattgcgaa tcgaccaggt gtcaaaaaag gcgtagaacc agatcaccgg
     5281 cagcaggatc gaaaagatcc cgatgttcaa aaagccactg ataccgatcg tcagcataaa
     5341 ggcggtcatc agcgagacgc cgcgcttggt gtagcccagg tacatctggc cggccccggg
     5401 ccagcaggcg aaaataaagg tggcaatagg atgctttttc atttgtctga ttctcctttt
     5461 tggttcagtg cgccaaacag ttggtcgatg gcgttggaaa tgcgcatgtt cagcgtgccc
     5521 tcgggcgcat cggcagtgtc cagctgttct ttcagttctt tttcagcggc ctcgttgcgg
     5581 cgctgctcct gctcaaggcg ctgggccgtg cggtcggggc ccgcggtgcc gatcgccgag
     5641 ggcagcaggg tgaagacgcc gctgaaccag agggtgaggg ccagacaggc ggccaggccc
     5701 actttcatag cacggcgaaa gaagatcacc cggccgtgcc gtttcacccg ctccatcacc
     5761 ggcggcgcca gcgggcgctc aggctccagc agcacctccc cggtgagcag gcccgcgtag
     5821 cgggcgcagc agctgtcaca gaaagacaga tgctcggcca cctcgaggct ggcgagttcg
     5881 tccagctccc cggccagcag ggcggcaaag ccctcggcgg tgatgtggcc ggtccgatca
     5941 tcaaacacgt ccacgggtta cccctccttt gataagtcgt tgttgcaaaa tctgttttgc
     6001 gcggtagagc tgggtgtgta cggttttggg gggccgcccg agccgtgcgg cgatctcatc
     6061 caccgagcgc tgttccagaa aatagagcac cgagacctgt aagtaaggtt ctttcagggc
     6121 ccgtatcagc ccctcgatct cgcggcgcat ggccgcgtcg accaccaggt cctcggtggg
     6181 cggcgaggcg ctgccgttgt actgcgcgtc ctcgtcgttg tcggtgaggt ccaccctgcg
     6241 gttgtaggcg cttttgagaa aatccttcgc cttattggtc gcgatgcggc aaagccaggg
     6301 cttggcggtg tccggtgtgc agctgtcgcg atgggtatag gctgagagaa aggtctcttg
     6361 ggcgaggtcc tcggcggtgt gcgggtcttt ggtcatctgg tagcagaccg tgtagaccag
     6421 cttttggtat ttttcaacga gctgttcaaa cgcctcatcc gtcaccaccg cctcaccgcc
     6481 tttcatgcat gttacgaccg gcccggaggc ctttcttcaa aaaaacgaaa aatttttgca
     6541 gagagaaaaa attttcctct cttttcctgc cccgcccgga ttttccgccg cgcggcgcgc
     6601 ccggggcaaa acaaaaccgg gggcgagacc gtctcgcccc cggcgcgttt tgtgcgcggg
     6661 tgggttgccg ctcaggcaaa aatcatgccc aggccgccga aaagccctgc cgaaagcgcc
     6721 cccatgatga cgcccgcggt gagcacgccg cacatgacgg ccagcacgct gggccagaac
     6781 ttcatgtcca gcagcgtggc cgccaggctg ccggtccagg cgccggtgcc gggcaggggg
     6841 atggcgacaa agagaaacag ggcgacatac aggccgcggc cggccttttc ctgcatcttg
     6901 tcgcccgcct tgaccccctt gctgtagatc cagttgaacg gcttgcccca ggccccgggc
     6961 agcctgcagc accacagcag cacctttttg ctgaacagga tgatgatcgg caccggcagc
     7021 atgttgccga tgatggccgt gatgtaggtg cccgccagcg gcagcccgct ggccacgccg
     7081 atggggatgg cgccgcgcag ctccacgatg ggcaccatcg agaccagaaa gacccagaaa
     7141 tattttttga acagtgtaag cataacaaaa cctcggcttg atgatggtat ccgcgcccca
     7201 cggggggcgg gacgctcccc tccccgcgca gcgcggcgga ggctgaaaca tggttagtat
     7261 acccgatttc ccgcaaaagg gcaagaaaaa caggtttttt acccgtgggc gccgcgggag
     7321 aggccgaacc caccccacca ggtgcccttg tcccgggggc ttggggcgag cgggcccggc
     7381 ccccgggctg cccccttgcc tcggccctcc ctgcctcggc cgcctttact ccgagtgctt
     7441 ttggagcggg gcggcctgcc ttggaaccct tgcggcgggc tgcccctgct ccgagtcccc
     7501 tgcggtgagc ggcccccgcc ctggcaccct tgcggtgggc cgccgggcgc tgtggggcct
     7561 cccgcttttt gaggcggacg gctgcggcgc gccggcggtg agacggcgca cgccgtccct
     7621 ccgcctcctc ccgccgccgg cgctgcgctt ttgcccgcgt ccccttgcc
//
LOCUS       DBMDYA010000036         7651 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2513_length_7651_cov_1.658411, whole genome shotgun sequence.
ACCESSION   DBMDYA010000036 DBMDYA010000000
VERSION     DBMDYA010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_7651_cov_1.658411"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="ACZPFK_01610"
     CDS             complement(<1..817)
                     /locus_tag="ACZPFK_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405036.1"
                     /translation="MNKKKGLLSAFLAAALALSLFAGAVGAATTPDNNATLTVSKPDQ
                     FTTGVATEFTVSTAKGTYDGEFVNGFVDFDRTKVEKLEYFESTTSNWQEMPGNTFGPE
                     SGFPLSDATSRFRVTFKDAGETPVTIKIVSLSNTVLLSETCTFTSVAPVAPTLTLAAP
                     DTFTAGVPAEFTVSTTKGDYAGGFVKGVGGITSGAEHVEKLEYYETAAGKEGWYELKG
                     DSFGPASGFPLMDATSRFRVTFKDAGKVAFSVKIVDVGTGAEVLSGSYTFTGVA"
     gene            complement(1175..2014)
                     /locus_tag="ACZPFK_01615"
     CDS             complement(1175..2014)
                     /locus_tag="ACZPFK_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1405037.1"
                     /translation="MKEHLSPRDIKSYVCHLRAEEKSAATIEKYARDTRQFFCFAAGR
                     RLDKELVIAYKSQLCAQYAPSSVNSMLAAVNSLLAYLGRADLRVKRLRIQRQIFCPKE
                     KMLDREEYRRLVLAAQARHDPRLALLLQTLCSTGIRVGELPYITAKAAQSGKAVVTSK
                     GKSRVVLLPKQLCARLRQYCRARGIASGCIFRSRSGRPLNRSNIWAAMKGLCAAAGVR
                     AEKVFPHNLRHLFARAFYSADKDISRLADILGHSSVETTRVYIVSSGLEHERQLAKLR
                     LLI"
     gene            complement(2099..2416)
                     /locus_tag="ACZPFK_01620"
     CDS             complement(2099..2416)
                     /locus_tag="ACZPFK_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405038.1"
                     /translation="MIALQMQKRLAIHRDCQPFLRCAFFKTIASKLPRLPRWKGRCRA
                     EKFTRLACSRRQSAKRSRKTRDGCRAGLTAFRAQLPPWGNVFLQKRARTGLFFSPFLP
                     AGF"
     gene            complement(2715..3995)
                     /locus_tag="ACZPFK_01625"
     CDS             complement(2715..3995)
                     /locus_tag="ACZPFK_01625"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1405039.1"
                     /translation="MPSKVVVGVQWGDEGKGKIIDILAQEAQVVVRAQGGNNAGHTVE
                     QGSEVYKLRTVPSGILYPDTLCLVGNGVVVNPGSLLAEMDGLEARGVSCKNLKIDPRA
                     HVIMPWHPLIDGLAEEARDAEHRIGTTRNGIGPCYMDKAERSGIRMWDLVHEEVFRKK
                     ARFAGEEKNRLITKLYGAEPMDLDKIIEEYVAYGKRLADHVEDVSVLVYNSYNEGKKI
                     LFEGAQGTLLDLDMGTYPFVTSSHPVAGGFAIGAGVGPRMIDEVIGVAKAYTTRVGEG
                     PFPTELFDEVGQLIRDKGHEYGTVTGRPRRTGWFDAVILRYAVRVNGLTQLAVNKLDT
                     LAGVGDLKVCTAYRLPDGTLLNEYPECLEVLADCEPVYETIPGFTEDISGCKSFDELP
                     ESCKSYIAELEHLCGCHITMVGVGPNREQNLTRK"
     gene            4271..4723
                     /gene="spoVAC"
                     /locus_tag="ACZPFK_01630"
     CDS             4271..4723
                     /gene="spoVAC"
                     /locus_tag="ACZPFK_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HIR1405040.1"
                     /translation="MKLSKQEYDQMNKQASPKSKLLPDCLWAFFVGGAICLLGEVLRT
                     LYGKWGFALTDAAGLTSATLVALSAVLTALGWYHKLATKAGAGTLVPITGFANAVVSP
                     AIEFRVEGLVTGVGAKMFVIAGPVIVWGMMASVIWGVIYYFIRPLMGG"
     gene            4734..5741
                     /gene="spoVAD"
                     /locus_tag="ACZPFK_01635"
     CDS             4734..5741
                     /gene="spoVAD"
                     /locus_tag="ACZPFK_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1405041.1"
                     /translation="MQNKKGDTLFFKTPPSVLGYAAVGGKKESEGPLADGFDVLNTDT
                     TFGEKTWEQSESRMQAICAETALKKSGVTPKELDYLFAGDLENQCTASNYTMRDLPVP
                     FLGLYNACATMAEGLGLAASFIAGGLAERTMALASSHFCAAERQYRFPLEYGGKRTPT
                     SQWTATASGGVVLGSVGGPPYVKSVTFGRVIDYGIKDINNMGAAMAPAAAHTLLTFLK
                     DSGRRPEDFDRIFTGDLGAVGSTLLEEICEKEGVKLTNHSDCGLLLYDREKQQVDAGG
                     SGVGCSASVLCSHILPQLKSGKLREVLFIATGALMSPTTFMQKESIPGIAHLVQLGSG
                     K"
     gene            5761..6117
                     /gene="spoVAE"
                     /locus_tag="ACZPFK_01640"
     CDS             5761..6117
                     /gene="spoVAE"
                     /locus_tag="ACZPFK_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HIR1405042.1"
                     /translation="MHFLWAFLVGGALCVIGQLLIDLTAMTPARILVLYVVAGVVLSA
                     LGWYLPLAEFAGAGATVPLLGFGHLLAQGVVDAVERHGLLGALTGGFSAAAGGIAASL
                     VCGVIASLLSRSKDQS"
     gene            complement(6220..6474)
                     /gene="rpsT"
                     /locus_tag="ACZPFK_01645"
     CDS             complement(6220..6474)
                     /gene="rpsT"
                     /locus_tag="ACZPFK_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1405043.1"
                     /translation="MPNIKSQKDRVRQAAKEAAHNKAIKTNLKTVVKKAEAAVSSGAA
                     DADKAVVAAVAAIDKAAGKGVLHKNTAARRVSRVMRAKKA"
     gene            6664..7470
                     /gene="gpr"
                     /locus_tag="ACZPFK_01650"
     CDS             6664..7470
                     /gene="gpr"
                     /locus_tag="ACZPFK_01650"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1405044.1"
                     /translation="MAIYTDIAAEVWAERKGTVPPGVRFETKYVADVTITRVHITRDG
                     LARRRGRYSTIDMPSFAYIDDRNEHYIGALAHEIKNYLPPKGDILVVGLGNDHITADA
                     LGPAVCGRILVTRHIPIERDERDILHLRGVSALCPGIEGKTGLSAGETVKSVVGAVRP
                     AAVLCIDSLFTSSPEHLGCTVQITDTGLCPGGEERKRIDTELLGVPTVCMGVPTVMDA
                     GDLCNSRHRLVVTPKEIDYIVSRAANVLSLAINKALQQELSVGELNFLTS"
BASE COUNT         1402 a         2356 c         2430 g         1463 t
ORIGIN      
        1 gggccacacc ggtaaaggta tagcttccgg agaggacttc cgcgccggtg cccacgtcta
       61 cgattttgac gctgaaagcg accttgcccg cgtccttgaa cgtgacgcgg aagcggctgg
      121 tggcgtccat cagggggaag ccggaggcag ggccgaaaga gtcgcctttc agctcgtacc
      181 agccctcttt gccggcggcg gtctcatagt actcgagctt ttccacatgc tccgcgccgg
      241 aggtgatgcc gccgacgccc tttacaaagc cgcccgcgta atcgcccttg gtggtggaga
      301 cggtgaactc agcaggcacg ccggcggtga acgtatccgg cgcagccagg gtcagggtgg
      361 gggcgacagg agccacactg gtgaaagtgc aggtctcaga taagagcacg gtgttgctga
      421 gggatacgat tttgatggtc accggcgttt cgcctgcgtc cttaaacgtg acgcggaaac
      481 ggctcgtggc gtctgacagg ggaaatcccg attccgggcc aaaggtgttc ccgggcattt
      541 cctgccaatt gctcgtcgtg gattcaaagt actccagctt ttccaccttt gtgcgatcga
      601 agtccacgaa gccattcaca aactcgccgt cgtaggtgcc cttggcggtg gagacggtga
      661 actcggtggc gaccccggtc gtgaactgat cgggcttgga gacggtcagg gtggcgttgt
      721 tgtccggggt ggttgctgcg cccaccgcgc cggcaaacag ggaaagtgcc agtgcggcgg
      781 ccaaaaatgc ggacagtaga cctttctttt tgttcatggt tcctccttta ccacataacc
      841 aacgttatgt ggtaaagaga cggggagagg ggaggaatga ctgcttgtgg ccccggcggg
      901 acgcagtgcg accgcgcatc cagacacaaa aaacagactt tctgtgcgct gttgaacaga
      961 aaatccgttt tttgcagtta aagttgaaat tttatcattg acaaaatttt cctgaaagag
     1021 taaattaata aaaagaagag taaaactctg acaaatttgg gaaagcacta cataacgttg
     1081 gttatgtggt gcttttgttt tgccgcaggc ccaaagggcc tctcgtgtag gctgctgcgg
     1141 ggcagggggc cgcccgcagc ggcttggcgc atgctcagat gaggagccga agctttgcca
     1201 gctgccgctc gtgttccagg ccgctggaga cgatgtagac ccgcgtcgtc tccacgctgg
     1261 aatggcccag gatgtccgcc agacggctga tgtccttatc cgcgctgtag aaagcgcggg
     1321 cgaacagatg ccgcaggtta tggggaaaga ccttttccgc tctcaccccg gccgctgcgc
     1381 agaggccttt catggcggcc cagatattgg agcggttcaa gggcctgccg ctgcgggagc
     1441 ggaagataca gccggaggcg atgccccttg cccggcaata ctgccgcagc cgggcgcaga
     1501 gctgctttgg cagcagtacc actcgtgatt tgcctttgct ggtgaccacc gctttgccgc
     1561 tctgggcggc ttttgcggtg atgtagggca gctctcccac ccggatgccg gtagaacaga
     1621 gggtctgcag aagcagcgcc aggcgcgggt catgccgcgc ctgtgccgca aggacaagcc
     1681 gccggtactc ctcccgatcc agcattttct ccttgggaca aaagatctgg cgctggatgc
     1741 gcagcctttt gacccgcaga tccgcgcggc ccaaataagc gagcaggctg ttcaccgccg
     1801 ccagcatgga gttcacgctg ctgggggcat actgcgcgca cagctggctt ttataggcga
     1861 tgaccagttc tttatccaat ctgcggcccg cggcgaagca aaaaaattgc cgtgtgtccc
     1921 gcgcatattt ttctatcgtc gcggcggact tttcttcagc gcggagatgg cagacatagc
     1981 tttttatgtc ccggggcgaa agatgctctt tcataaatgg gcctccgatc cacttttttc
     2041 ctctattata caggacttga ttttccacgg atattggtaa aataaggaaa tcagcgcttc
     2101 aaaagccagc cggaagaaaa gggctgaaaa aaagccctgt ccgggcgcgt ttctgcaaaa
     2161 aaacattgcc ccaagggggg agctgtgcgc ggaacgccgt caaacccgcc cggcagccgt
     2221 cgcgggtttt gcggcttcgc ttcgcggact gcctgcgcga gcaggcaagc cgcgtgaatt
     2281 tttcagcccg gcatcgccct ttccatcgcg ggaggcgcgg cagttttgaa gcgatggttt
     2341 tgaagaaagc acagcgcaaa aaaggctggc aatctctgtg gattgccaac cttttttgca
     2401 tctgcagggc gatcaaaccg ccgctccagc gcccctgttt ttttacctca acacgccgaa
     2461 agcggcggtg gctgatcgaa cggtcctttg ggggccgtcc cgaccacacg cccccaaaat
     2521 cttgagcgcc cgcccataaa gggggcgggc gctctcaaaa aacaactgct cgcaacggcc
     2581 ccgtttcccg ccgctgcgcg gcaaccgaag cgaatggcca ataaaaatag ccgctgcgcg
     2641 gctattttta ttgctatggc cgtgggccaa ggatgggcca caggactaca acatagccgc
     2701 caagcggctc tgttttactt tctggtcaaa ttctgctccc ggttggggcc cacgcccacc
     2761 atggtgatgt ggcagccgca caggtgctcc agctcggcga tatagctctt gcagctctcg
     2821 ggcagctcgt caaagctctt gcagccggag atgtcctcgg taaagccggg gatggtctca
     2881 tagacgggct cgcagtcggc cagcacctcc aggcattccg gatactcgtt cagcagggtg
     2941 ccgtcgggca gccggtaggc ggtgcagact ttcagatcgc ccacgcccgc cagcgtgtcc
     3001 agcttgttga cggcgagctg ggtcaggccg ttcacccgca ccgcatagcg caggattacc
     3061 gcgtcgaacc agccggtgcg gcgcggccgc ccggtgacgg tgccgtactc gtgccccttg
     3121 tcgcggatga gctgccccac ctcgtcgaac agctcggtgg ggaaaggacc ctcgcccaca
     3181 cgggtggtgt aggccttggc cacgccgatg acctcgtcga tcatgcgcgg gcccacgccc
     3241 gcgccgatgg caaagccgcc cgccaccggg tggctgctgg tgacaaacgg gtaggtgccc
     3301 atatccaggt ccagcagggt gccctgcgcg ccctcgaaga gaatcttttt gccctcgttg
     3361 tagctgttgt aaaccagcac cgaaacgtcc tccacgtggt ccgccagccg cttgccgtag
     3421 gccacgtact cctcgatgat cttgtccagg tccatcggct cggcgccgta cagcttggtg
     3481 atcaggcggt ttttctcctc gccagcaaag cgcgcctttt tgcggaagac ctcttcatgc
     3541 accagatccc acatgcggat gccgctgcgc tcggccttgt ccatatagca ggggccgatg
     3601 ccgttgcggg tggtgccgat gcggtgctcc gcgtcccgcg cctcctcggc caggccgtcg
     3661 atgagcgggt gccagggcat gatgacgtgg gcgcgcgggt cgatcttcag gtttttgcag
     3721 ctcacgccgc gagcctccag gccgtccatc tcggccagca ggctgcccgg gttgaccacc
     3781 acgccgttgc ccaccaggca gagcgtgtcg ggatagagga tgcccgaggg cacggtgcgc
     3841 agcttgtaga cctcgctgcc ctgctcgacg gtgtggccgg cgttattgcc cccctgcgcg
     3901 cggacgacga cctgcgcctc ctgcgcaagg atgtcgatga tcttgccctt gccctcgtcg
     3961 ccccactgga cgcccacaac aacctttgac ggcataattc tttttccccc tatagtcctt
     4021 gaaagcggcc gggcgcaccc gcccggcggc cggaatgagc gcgggcgccc cgcgcccgca
     4081 cgaacaaacc ctattatacc agaccccgtc ccgattgcaa accgtccccc cgcattttga
     4141 gcaaaatttc ccgggaaaaa cgcgctcggg ggacataatt ttcccacgcg ggggaaatac
     4201 tggctttaaa gcgcaacgcc gggctagccc cgggccgcgc gtaaaacttt acggggggat
     4261 ccgaaatctt atgaagctct ccaaacagga atacgatcag atgaacaagc aggccagccc
     4321 aaaatcaaag ctgctgccgg actgcctgtg ggcctttttc gtgggcggcg ccatctgcct
     4381 gctgggcgag gtgctgcgca ccctgtacgg caagtggggc tttgcgctga ccgacgcggc
     4441 gggcctgacc agcgccacgc tggtggcgtt gtcggcggtg ctgaccgcgc tgggctggta
     4501 ccacaagctg gccaccaagg cgggggcggg cacgctggtg ccgatcaccg gctttgccaa
     4561 cgccgtcgtc tcgcccgcca ttgagttccg ggtcgagggc ctggtcaccg gcgtgggcgc
     4621 aaagatgttc gtcatcgccg ggccggtcat cgtctggggt atgatggcaa gcgtgatctg
     4681 gggcgtgatc tattacttca tccgccccct tatgggaggt tgacgggatg cagatgcaga
     4741 acaaaaaagg cgatacgctg ttttttaaaa cgccgccgtc ggtgctgggg tacgcggcgg
     4801 tgggcggcaa gaaagagtcg gaggggcctc tggccgacgg ctttgacgtg ctgaacaccg
     4861 acaccacctt tggggagaag acctgggagc agtcggagag ccgcatgcag gccatctgcg
     4921 ccgagacggc cctgaaaaag agcggcgtca cccccaaaga gctggactat ctgttcgccg
     4981 gcgacctgga gaaccagtgc acggccagca actacaccat gcgcgatttg ccggtgccct
     5041 ttttgggcct gtacaacgcc tgcgccacca tggccgaggg gctgggcctg gcggcgtcct
     5101 ttatcgcggg cggcctggcc gagcgcacca tggccctggc ctccagccac ttctgcgcgg
     5161 ccgagcggca gtaccgcttt ccgctggagt acggcggcaa gcgcaccccc accagccagt
     5221 ggacggcgac ggcctccggg ggggtggtgc tgggcagcgt gggcggcccc ccttacgtca
     5281 aatcggtgac ctttggccgg gtgatcgact acggcatcaa ggacatcaac aacatggggg
     5341 ccgccatggc cccggccgca gcccacacgc tgctcacctt tttaaaggac agcggccgcc
     5401 gccccgagga ctttgaccgc atcttcaccg gcgacctggg cgccgtgggc agcaccctgc
     5461 tggaggagat ctgcgaaaaa gagggcgtca aactgaccaa ccactccgac tgcggcctgc
     5521 tgctctatga ccgggaaaaa cagcaggtgg acgcgggcgg ctccggcgtg gggtgcagcg
     5581 cctcggtgct ctgcagccac atcctgcccc agctcaaaag cggcaagctg cgcgaggtgc
     5641 tgtttatcgc caccggcgcg ctgatgagcc ccaccacttt tatgcagaaa gagagtatcc
     5701 ccggcatcgc ccatctggtg cagctgggga gcggaaaata aggaaaggcg gaacctgatc
     5761 atgcattttc tctgggcatt tctggtcggc ggcgcgctgt gcgtcatcgg ccagctgttg
     5821 atcgacctca ccgcgatgac cccggcgcgc atcctggtgc tctatgtggt ggcgggcgtc
     5881 gtgctgtcgg ccctgggctg gtatctaccg ttggccgagt ttgcgggcgc gggcgccacc
     5941 gtgccgctgc tgggctttgg gcacctgctg gcacagggcg tggtggatgc cgtggagcgc
     6001 cacggcctgc tgggcgcgct caccggcggc ttttccgccg ccgcgggcgg cattgccgcc
     6061 agcctggtct gcggcgtcat cgcctcgctg ctgtcgagga gcaaggacca gagctgagcg
     6121 ggcccgacgc ggcctgacgc cgccctcgcc cggcgcatcc cggaaagaaa caaaaaagaa
     6181 aggccgccgc gattgcggca gcctttctgc tgggtttgct tacgctttct ttgcgcgcat
     6241 cacacgggag acgcggcggg cggcggtgtt cttgtgcaga acgcccttgc ccgcggcctt
     6301 gtcgatggcg gcaacggcgg cgacgacagc cttgtcagcg tcggcggcgc cagaggagac
     6361 ggcggcctct gccttcttga caactgtttt caggttggtc ttgatggcct tgttgtgggc
     6421 ggcctctttg gcggcctgac gcacacggtc tttctgggat ttaatgtttg gcataatacc
     6481 acctccttta ttaaccataa cagtgctctt tatattatca tacttttcgg gtgcaataca
     6541 agggcttttt gaaaaaaatc aaaataaaat gcggaaaagg gccgcagggc cgccgttttc
     6601 cccttttccg gagagggagg gccggtgcgg cgggcggatt ttccggcgag aggaagatgt
     6661 gatatggcga tttacaccga catcgcggcc gaggtctggg ccgagcgcaa gggcaccgtg
     6721 ccccccggcg tgcgctttga gaccaaatat gtggccgacg tgaccatcac ccgcgtgcac
     6781 atcacgcggg acgggctggc ccgccgcaga gggcgctatt ccaccatcga catgcccagc
     6841 tttgcctata tcgacgaccg caacgagcac tacatcggcg ccctggccca cgagatcaaa
     6901 aattatctgc cgcccaaggg ggacatcctg gtggtgggcc tgggcaacga ccatatcacc
     6961 gccgatgcgc taggcccggc ggtctgcggg cgcatcctcg tcacgcggca catccccatc
     7021 gagcgggatg agcgggacat cctgcacctg cgcggcgtct ccgcactctg cccgggcatc
     7081 gagggcaaga ccggcctctc ggcgggggag acggtgaaga gcgtggtggg cgccgtgcgc
     7141 ccggcggcgg tgctctgcat cgacagcctg ttcacctcgt cgcctgagca cctgggctgc
     7201 accgtgcaga tcaccgacac cggcctgtgc ccgggcggcg aggagcgcaa gcgcattgat
     7261 acagagctgc tgggggtgcc caccgtctgc atgggggtgc cgacggtgat ggacgcgggc
     7321 gacctctgca acagccgcca ccggctggtg gtcacgccca aggagatcga ctacatcgtc
     7381 tcccgggcgg ccaatgtgct cagccttgcc atcaacaagg ccctgcagca ggagctcagc
     7441 gtgggagagc tgaacttttt gacctcttga gccaaagcgc tctttgagga gccccagggc
     7501 cgacagaggc cggagagact gccggcgtcc gtcttcgcgc aacgcccgtc ccctgaggcg
     7561 gcggcagggc cttgccgcac cgctcccgac tcgtgcgcgg gacggctctc agcgcggcgg
     7621 cgtttttggc tttttataga acagcggcgc g
//
LOCUS       DBMDYA010000037         7642 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2516_length_7642_cov_1.532423, whole genome shotgun sequence.
ACCESSION   DBMDYA010000037 DBMDYA010000000
VERSION     DBMDYA010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_7642_cov_1.532423"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="ACZPFK_01655"
     CDS             complement(<1..584)
                     /locus_tag="ACZPFK_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405045.1"
                     /translation="MRQVIEKMNYSTDRSSFMTRTMRSRSIALVLPDITNPFFADIAK
                     AVEARAKQEDYITILANTDFSERNEIKYLASLVAKKIDGIILVPSGVHTRNEHLTPEK
                     YGIPMILLDRNLAGDHDWLGVYSDNEYASFRSCETLINNGSDRIAFLSGPLGVSTSTE
                     RLEGYKLALKQYGSGYDPHLVKYGDYTVESGYKAV"
     gene            complement(560..856)
                     /locus_tag="ACZPFK_01660"
     CDS             complement(560..856)
                     /locus_tag="ACZPFK_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012576.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405046.1"
                     /translation="MAEFHRKWLFGSAFDPFLPHQQNYTRAIVDIWTLILFTKQVFSN
                     KVKTNETNAIGNRDMKVTIEEIAKIAGVSKATVSRVLNDVPEGVGPGCGRSLKR"
     gene            1397..1741
                     /locus_tag="ACZPFK_01665"
     CDS             1397..1741
                     /locus_tag="ACZPFK_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1405047.1"
                     /translation="MTNTLDYEKLRTQIRIRRVRNHWRQADLAERIDVTPAYISRIET
                     GKDRPSLGILYRMSQAFECTVDDLIRADGQESGSYLIATIKSRAEKASDPQRRKILRM
                     IELLLAEDVSVD"
     gene            1761..2333
                     /locus_tag="ACZPFK_01670"
     CDS             1761..2333
                     /locus_tag="ACZPFK_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1405048.1"
                     /translation="MLFLMLFADPEERDLFAGLFKKYEGALRSRALKIVHNPCDAEDC
                     VATAFEHVGRLFADRPGMLKEFGETRAVSYLATTVANEAKNLVNSSAHRTKADFSYED
                     APGLTGGGPSAEELAIRREDYRQLVQLVNGLCEKYRTPLVMHFYLELSDREIAQALSI
                     TENYVSVPCLRGRKILQRQLKELREKEGQV"
     gene            2330..3076
                     /locus_tag="ACZPFK_01675"
     CDS             2330..3076
                     /locus_tag="ACZPFK_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025643.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR1405049.1"
                     /translation="MSVKEPSSFDDELFELMLRHAVIESAQAEAQQVMDEAQADEEPF
                     PPSERYLRRIEKAHRGQKRALFAHRHRRALKIAASVLLVLFGLAATSFVTVSAVRSGF
                     LNFLLGEASDHTKVMLSDAAEGYDLAHLPEGWDAIYIPTYLPEGFQLSKVTMGKEAVI
                     AHYTNTKNEYITIIQWNRTDISLFYDNEHGELTPNTINGQEAVMRCDGYDTSISWTEN
                     NYTFTISSSLSQNKLILVCESLKKYEKELS"
     assembly_gap    3110..3119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3120..3552
                     /locus_tag="ACZPFK_01680"
     CDS             <3120..3552
                     /locus_tag="ACZPFK_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405050.1"
                     /translation="TMIKKSVSICLTVLLLFLALCIPVGAAVASEENIMEPRYTHFTV
                     VSINLSIDSLGCATATGTIQGRPGVTTGIKAVLQKYNSSNGTWSDIKTFNASSTDYNC
                     LLSGSYYVARGTYRVYAEFYAFNNTGVCVEIASGYSPIKDY"
     gene            4088..4339
                     /locus_tag="ACZPFK_01685"
     CDS             4088..4339
                     /locus_tag="ACZPFK_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1405051.1"
                     /translation="MSINRSFSGKLYDYRVRHGLTQKEMAELCGLSLRHYQDLEMGRS
                     LPKLENAVRMAAILDISLDSLKGEFRGEVESIDISLHDL"
     gene            4314..4547
                     /locus_tag="ACZPFK_01690"
     CDS             4314..4547
                     /locus_tag="ACZPFK_01690"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1405052.1"
                     /translation="MTYRYTIFEQQLFHEHIGYYRTFGIEGCVQTGDGWQPCIRLPDV
                     SVDREFTRRLAGRCTSSQLSPVHLKDAVLDALP"
     gene            complement(4702..5829)
                     /gene="tig"
                     /locus_tag="ACZPFK_01695"
     CDS             complement(4702..5829)
                     /gene="tig"
                     /locus_tag="ACZPFK_01695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1405053.1"
                     /translation="MNLTKKTLAFLLAAVLSAGVFAGCGGGSSSSSTGTSAGGVGGTD
                     FNYSDGLDEHGRWAGVTALDLVKLGDYKGVEVPADVHTVKEEDVDAQVQSMLSSYATT
                     NQVLDREVKDGDTVNIDYVGKVDGAEFEGGSTGGNGTSVTIGVTTYIDDFLEQLIGHK
                     PGDSFDVEVTFPDNYAPNPDLSGKDAVFATTVNYIEEKITPELNDEFVKEHLTKTYGW
                     KTADEVLAQVRSDLQWNATAQYLWDKLLDEAEVSEVPQSMIDYQLDRTVAEYGLAAQS
                     YGMTLEQALSSVGVSTKEELGKKYADDLKEQARQGLVMQAVAEDAAIDVGEEDVAAYF
                     KEFMYLDDYSSYTKNYGDGYVRMTVLNRKVLNYLVDNAVLA"
     gene            6121..6468
                     /locus_tag="ACZPFK_01700"
     CDS             6121..6468
                     /locus_tag="ACZPFK_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405054.1"
                     /translation="MDVLLIVACFYFAFSQAKALVDYGNQPWGVVHYVLLILTIALAG
                     LGLWRSVLYYKEWKYKKEHPEEAEVLGAGEAVEEDEAEDGGDEEAADEAVDVAALPDA
                     EKPADGEEPKDGQ"
     gene            6768..7256
                     /locus_tag="ACZPFK_01705"
     CDS             6768..7256
                     /locus_tag="ACZPFK_01705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1405055.1"
                     /translation="MKADLEQQELEQFRSLAGEVLANPLVRGMSAYIQHGQTSCLAHS
                     VAVAWYSFVVYKRLGLHGQERALVRGALLHDFFLYDWHEPGHPRLHGFTHPGAAARNA
                     VRYFGISGLEEDIIRKHMWPLTVIPPLHRAALIVSLVDKVCTVAEVFGHSYRPLLTAC
                     RV"
BASE COUNT         1562 a         2174 c         2083 g         1813 t
ORIGIN      
        1 accgccttgt agccgctctc caccgtgtag tcgccgtact tgaccaggtg cggatcgtag
       61 ccgctgccgt actgcttgag cgccagcttg tacccctcca gccgctcggt gctggtggag
      121 accccaagcg ggccggacaa aaacgcgatg cggtccgagc cgttgttgat gagggtctcg
      181 caggagcgga acgacgcgta ttcgttgtcc gagtaaacgc ccagccagtc gtggtcgccc
      241 gccaggttgc ggtccagcag gatcatcggg atgccgtatt tttccggcgt caggtgctcg
      301 tttctggtgt gcacccccga gggcaccaga atgatgccgt cgatcttctt tgccaccagg
      361 ctcgccagat acttgatctc gttgcgctcg gaaaaatcgg tattggcgag gatggtgatg
      421 taatcctcct gctttgcccg cgcctcgacg gccttggcga tatccgcgaa aaacgggttc
      481 gtgatatccg gcagcaccag tgcgatgctg cgcgagcgca tcgtgcgggt catgaagctg
      541 gagcggtcgg tggagtagtt catcttttca atgacctgcc gcacccgggc ccgaccccct
      601 ccgggacgtc gttcaggacc ctggaaactg tggctttgga aactccggcg atctttgcaa
      661 tttcttcgat agttactttc atatccctgt ttccaatcgc atttgtttcg ttggttttaa
      721 ctttattgga gaaaacttgt ttcgtaaaca aaattaatgt ccaaatatca actattgctc
      781 ttgtgtaatt ttgctgatgt ggcaaaaacg gatcaaaagc gctgccaaat aaccattttc
      841 tgtgaaattc cgccaaatcg cttcctctgc gcgccacact tttatgtctt tattttcgtc
      901 tttaaaggta aaagggtgcg ccgcaaacgc ctgggccccg ccctgtacac ctctcttttg
      961 tcgaggtgtt ttccttgctc ttttctaaaa tcatgagcaa catagcttca aatatgggta
     1021 ttgaaagaag cttttctcca tttgtttctt ttttgttact tttgagcctt ttgttttccc
     1081 ttttgcggag aaacagggct ttcacttgta ttttgcctga ttctcttgct ataatgtggt
     1141 gcagcacccg gcgctgcggt ggggcgcgcg gcgcggaaca aaaagaaaag ccgcggtcct
     1201 ggccgcagct tttttagaaa tcctgcgctt gaagcggttt ttgagatcaa ctgtcaagtt
     1261 aagtgttctt tctgattttt gcggataaaa tagtcaaaaa actttgttaa tattactttt
     1321 aatggcaact aattaagtta tactgcattt atagatgcgg tcattcttgt taaccttaaa
     1381 tcaggaggtg aatggaatga ccaatacgct cgactacgaa aagcttcgca cccagatcag
     1441 gatccggcgg gtgcggaacc actggcggca agctgatctg gccgagcgca tcgacgtgac
     1501 ccctgcctat atcagccgca ttgaaacggg caaagaccgc ccctctctgg gcattctgta
     1561 ccgcatgtcg caggcttttg agtgcacggt ggacgatctg atccgcgcgg acgggcaaga
     1621 gagcggcagt tatctgatcg ctaccatcaa gtcccgcgcc gaaaaggcca gcgacccgca
     1681 gcggcgcaag atcctgcgga tgatcgaact tcttttggca gaggacgtca gcgtggatta
     1741 gaggaaagcg gagtgaaagt ttgctgtttt tgatgctgtt tgcagacccc gaggagcggg
     1801 acctgttcgc cggcctcttc aaaaaatatg agggcgcgct gcgctcaagg gccctcaaga
     1861 tcgtacataa cccctgcgac gcggaggatt gtgtcgccac tgcctttgag catgtggggc
     1921 ggttgtttgc ggacaggccc ggcatgctga aagaatttgg tgaaacccgc gcggtgagct
     1981 acctcgccac cacggtggcc aacgaggcga aaaacctggt gaacagcagc gcccaccgca
     2041 ccaaggccga tttttcttat gaggatgccc cgggcctgac gggcggaggc ccctccgccg
     2101 aagagctggc catccggcgg gaggattacc gccagctggt gcagctggtc aacggcctgt
     2161 gcgaaaagta ccgcacgccg ctggtgatgc acttttatct ggagctgtcc gaccgggaga
     2221 tcgcacaggc gctttccatc accgaaaact acgtctcggt gccctgcctg cgcggccgca
     2281 aaatcctgca gcggcagctg aaagagctgc gtgaaaagga gggtcaggta tgagcgtgaa
     2341 agaaccgtcc tcctttgacg acgagctgtt tgagctcatg ctgcggcatg cggtgatcga
     2401 gtcggcgcag gcggaggcac agcaggtgat ggacgaggcg caggcggacg aggagccgtt
     2461 tccgccgtcg gagcggtatc tgcgccggat cgaaaaggcg catcgaggcc agaagcgcgc
     2521 gctgtttgcc caccgccacc ggcgcgcgct gaagatcgcc gcctcggtgc tgttggtgct
     2581 gttcgggctg gcggccacct cgtttgtgac ggtgagcgcc gtgcgctcag gctttttgaa
     2641 ctttttgctg ggcgaggcat cggaccacac caaggtcatg ctcagcgacg ccgccgaggg
     2701 ctacgacctc gcccacctgc cggagggctg ggacgcgatc tatatcccca cctatctgcc
     2761 ggaggggttt caacttagca aggtcactat gggcaaggaa gccgtcattg cccattacac
     2821 taatacgaaa aatgaataca tcacgattat tcaatggaat agaacagata tttctttatt
     2881 ttatgataat gaacatgggg aacttacccc taacactata aacggtcaag aagctgtaat
     2941 gcgctgtgac ggctatgata cttctatttc ttggacagaa aataactata cttttactat
     3001 cagttcctct ctttcccaaa ataaattaat acttgtctgc gagtctttaa aaaaatatga
     3061 aaaagagtta tcataaaaaa aatgtttttc caatgttagc aaggaggttn nnnnnnnnnc
     3121 accatgatta aaaaatctgt tagtatttgt ctcactgttc tgttactatt tcttgctctc
     3181 tgtataccag ttggtgccgc ggtagcatcc gaagagaaca taatggagcc gcgatataca
     3241 cattttacgg tagtctctat taacctttct attgattctc taggatgcgc aactgcaaca
     3301 ggaactatcc aggggcgtcc cggtgttaca accggtatca aagctgtact tcaaaagtac
     3361 aacagcagca atggtacttg gagtgatatt aaaacattta atgccagcag cacagattat
     3421 aactgtctgc tttctggctc ctattacgtg gcgcgcggca cttatcgcgt ctacgctgag
     3481 ttctatgcat tcaacaatac cggcgtctgc gtggagattg ccagcggcta ttcccccatc
     3541 aaagactact aaacgctttt cccgtcccac cgtgcgcggc tgccatggcc gcgcactttt
     3601 ttattgctcc gcaataaaaa acgacctatt tctatggccg tggccagagg actggccaca
     3661 tggccgaaaa atagcctatg gctattttta ttgcgttgat ttctactttt ttctccctct
     3721 cctttcccat ctttcctcca tgcatcctcc tgccgtcccc gcgcttccac gttaccacct
     3781 ttcccctccc tcgcggccgc ttctgcggcc gctttttgtg ttcttttgct ctccgcatat
     3841 caaaatcaaa acttttttaa aataattgtc gtttttgtgt catattttgt cttttgattt
     3901 gtctatacta tgccagcctc tctccagagc ggcaagaccg ttttttcgtt ttccgtgtcc
     3961 agaagccaaa agggggtttt aagatgataa attgcaggct gaaaaatgag gcgttttcca
     4021 caataggaag tacggctcgt tttctcccgc cgcaatgcgg gctaaaatcg ccgaggggag
     4081 gattttcatg tctatcaaca ggagcttttc ggggaagctg tacgactacc gggtccggca
     4141 tggactgaca cagaaagaga tggcggagct ttgcggtctt tcgcttcgcc attatcaaga
     4201 cctggagatg gggcgttctc tgccaaagct ggagaacgcc gtgaggatgg cggctatttt
     4261 ggacatcagt ttagattccc tgaaagggga attcagagga gaggtcgaat ctattgacat
     4321 atcgctacac gatctttgag cagcagctct ttcacgagca catcggctat taccgcactt
     4381 tcggcatcga gggatgcgtg cagaccggcg acggctggca gccctgcatc cggctgccgg
     4441 acgtctcggt cgaccgcgag tttacccggc gtctggccgg gcgctgcacc agcagccagc
     4501 tctcgccggt ccacctgaaa gacgccgtgc tggatgcgct cccctaaaag cgtcccggca
     4561 ggcaaaagcc tcccgccccg ctcccggcgg gcctcgcctc tctcccccct cgtgccgtct
     4621 tttttgggca cagtgaaaac aaaaagcgga aagcagcctc ggcgagaggc cgctttccgc
     4681 ttttttatat ttggctgccc gtcaggccag cacggcgtta tccaccagat aattcagcac
     4741 cttgcggttg agcaccgtca tgcgcacata gccgtcgccg tagtttttgg tgtagctgct
     4801 gtagtcgtcc agatacatga actccttgaa ataggcggcc acatcctctt cccccacgtc
     4861 gatggccgca tcctcggcca ccgcctgcat caccagcccc tggcgggcct gctctttcag
     4921 gtcgtcggca tactttttgc ccagctcctc cttggtggac acccccaccg aggacagggc
     4981 ctgctccagc gtcatgccgt agctctgggc cgccaagccg tactcggcta cggtgcggtc
     5041 caactggtag tcgatcatgc tctggggcac ctcgctcacc tcggcctcgt ccaacagctt
     5101 gtcccacagg tattgggccg tggcgttcca ctgcaggtcg ctgcgcacct gcgccagcac
     5161 ctcgtcggcg gtcttccacc cgtaggtctt ggtgagatgc tctttcacaa attcgtcgtt
     5221 cagctccggg gtgatctttt cttcaatata attgacggtg gtggcgaaca ccgcgtcctt
     5281 gccgctcaga tccgggttgg gagcgtaatt gtcggggaaa gtgacctcca cgtcaaagct
     5341 gtcgcccggc ttgtggccga tcagctgctc aagaaaatcg tcgatgtagg tggtgacgcc
     5401 gatggtcacg ctggtgccgt tgcccccggt gctgccgccc tcaaactcag ctccatccac
     5461 cttgcccaca tagtcgatgt tgacggtgtc gccgtccttc acctcccgat ccagcacctg
     5521 gttggtggtg gcatagctgg acagcatgct ctgcacctgc gcgtccacat cctcttcctt
     5581 gacggtgtgc acgtcggcgg gcacctcgac gcccttgtaa tcacccagct tcaccaggtc
     5641 gagggcggtg acgcccgccc agcggccgtg ctcgtccaac ccgtcgctat agttaaaatc
     5701 tgtgccgccc accccgccgg cggaagtgcc ggtggacgag ctggacgagc cgccgccgca
     5761 accggcgaac acgcccgccg acagcacggc ggcaagcaga aaggcaaggg tctttttggt
     5821 caggttcata ctgcaactcc tcaaaataat ttttgcccgc gccgcgccgc gcgcgccggg
     5881 gcgagccctg cgggcgcagg gccgataaat tgtattatac accttttgac ggtaaaatga
     5941 gtaggccttt tgttaatttt cacctatccc gcccctctcg cgccctctgg ggggatgccg
     6001 caggggcggg cgacgacgcc attttcggct tttgtgccaa attccttgcg cgcgtgtcaa
     6061 aaatcccgta taataacatt cagtgtcaaa ctgaaaaatt actgtcaagg gagatttcct
     6121 atggatgtac ttttgatcgt cgcctgcttc tattttgctt tcagccaggc gaaagccctc
     6181 gtcgactacg gcaaccagcc ctggggcgtg gtgcactatg tgctgctgat cctcaccatc
     6241 gccctggcgg gcctgggcct gtggcgctcg gtgctctatt acaaggagtg gaagtacaaa
     6301 aaagagcacc ccgaggaggc cgaggtcctg ggcgccgggg aagccgtcga ggaggacgag
     6361 gcggaagacg gcggggacga ggaagcagcc gacgaggctg tcgacgtcgc cgccctgccc
     6421 gacgcggaga aacccgcgga cggcgaggag ccgaaagacg ggcaatgact tttctgcgcg
     6481 ggctgtcacc ggacggcggc ggccccgcgc ggaccccagg ggcggcaggc cccgctttgc
     6541 gcgatcagat cggaacgtgg ccctgtgcgc cgccgggtgg agctttttcg cccgcagcgg
     6601 cgcgcggggc ttttttcatc gttttttctt tgtattttgc cgcctcgcgg cctataatag
     6661 aggcggaaac gcgcgattgg agcagcggct tgaagttttt ggccccgcga gatcgtctta
     6721 taggcaaaac ggcgttgtcc cgccggaaag cagagagggg acccaagatg aaagcagacc
     6781 tggagcagca agagctggaa cagtttcgca gccttgcggg tgaggtgctc gccaaccccc
     6841 tggtgcgcgg catgtccgct tatatccagc acgggcagac cagctgcctg gcccacagcg
     6901 tggcggtggc ctggtacagc tttgtggttt acaagcgcct gggcctgcac gggcaggagc
     6961 gcgccctggt gcgcggggcg ctgctgcacg atttctttct ctacgactgg cacgagcccg
     7021 gccatccgcg cctgcacggc ttcacccatc ccggcgcggc ggcccgcaac gccgtgcgct
     7081 atttcggcat cagcgggctg gaagaggaca tcatccgcaa gcacatgtgg ccgctcaccg
     7141 tcatcccgcc gctgcatcgg gccgcgctca tcgtctcgct ggtggacaag gtgtgcacgg
     7201 tggcggaggt attcggccac agctaccgcc ccctgctgac ggcctgccgg gtctgagccc
     7261 cgcgcactcg tctccacacg ccgctacaaa gacatccgca cagggcggat gtcttttttc
     7321 gtctcgtcaa aggcagccgc cgcttcgctg cggctatttt ttattgctcc gcaataaaaa
     7381 acaacctatc ttgatggccc tgtttctcgc cgccgtcgcg gcaaccgaaa cagatggccg
     7441 aaaaaatagc cgcgttgcgg ctatttttta ttgctgcgca ataaaaaata acttacccta
     7501 atggccacgt ttctcgccgc tgcgcggcaa ccgaaacgca tggccaaaaa aatagcctgc
     7561 ggctattttt tatttccatc gggtggccgt atttttgttg cttttctttt ttcaattaaa
     7621 aaaatgccaa ttccgaaaat aa
//
LOCUS       DBMDYA010000038         7496 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2563_length_7496_cov_1.475186, whole genome shotgun sequence.
ACCESSION   DBMDYA010000038 DBMDYA010000000
VERSION     DBMDYA010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_7496_cov_1.475186"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4345
                     /locus_tag="ACZPFK_01710"
     CDS             <1..4345
                     /locus_tag="ACZPFK_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1405056.1"
                     /translation="MKKKITALILAAAMVASIFAGCGSGPAPGESSSQNIGAGAGETL
                     ILSEPGNYTEKKEYGDVVLKADGIVLENALIHGSLTVDKTVGEGDATLRGCTVEGDVY
                     LYGGGSHSVHFENCILNTLIAEKKGLRVVLDSRTQTGAVSVRASARFEISAAVERVLI
                     ETGAADSELLFTEEAQASKVTLNAAARLEQNCPLEELNVGRKASGSSLLLSAKTEKVV
                     LSGACSVEVQADVGELVLTGNASGSEVAVAGGAQVDTLATSVPLTLSGEGTVKRAISD
                     EKAKLGGDLVPATVKEKEAPVLEADGTASANLATPTKAPTATAAPTKKPVPMPVPTPW
                     NPGTVTTPTAAPTATATPAVTSTPTPSPAPTPTPTPTATPVLVWDGTADTSWYTGDAQ
                     SYVLQSAEQLAGLADLVNGGNDFAGVTVTLGVSLDLDGREWTPIGRSSRSGGGLAAGS
                     MPFAGVFDGNGKTIAGLAISATSGADAGVGLFGAVAGSGAAVKGLTLTDVKVEVASSK
                     ALGAVTGLLTDGAMVSDCHVKGGTVSGTQGVGGIVGRGLKTGVVENCSNAAVVTASSA
                     NAGGIAGAMYYDQDPAGAGDRAPFAVRGCENRGAVKGQSVAVGGIVGLSTTCDISNCT
                     NSGTVTGGGTSVGGIAGEQKFGGTVSGCTNKAAATVSGNQYVGGIVGWVRYNGAGGTY
                     NTSLSVTVKDCVNEAPVEGTNCVAGIVGDIYWSCTLTGCTNSGAITSTGMGAGVAVEQ
                     SLSAGGGKDDNYSTARANRVEVSGCVNSGAVNTSPRAEILYVNSSDGDGCTVVVDGVK
                     LVDGDKILAITDGTNIYGTLAEAVAGAPDGATISLPEGRYDETVTTAKTLTIRGSGTG
                     KTVLAPSASGARAIVVDRGCTSTTAAAAAAAGSLTLEHLSMELPAQGRGVLVENTSNY
                     TVRLKDVDASTKDTGTAFLYLRGEVVGGEKRVQRDLFVEAEGFRLNAAGDLTIDRIFS
                     AFYFGNLTGGGLRLQNCDVRGARYAVNLQQSAGLAVEVTASTVQGWTTLNAISNNCTF
                     EVNGSTLIGYNSMTGGSNNYSVLVIDGGQIIGVDPVQGYTNTLHVAGSTLRAVRAPGC
                     CVEKYLSIQYSATQNQAVFENVAFEAENDPLGGGSGLDVGPGDRLAVNGVEVSNAAPQ
                     FTKEDDGDYWLLKVSSAGAVTGEWYDDGTKATISTDTALHILKTEAESRKVWGYFSAV
                     TEAAGQKCSVTCDLDIRQIAETNIPVEGIAFTGESGDAATLAVDEARRFSVVLQPADT
                     THRTVTWTSSDESVATVAADPQDPASAQVTALTAGQTVLSATAGGVTAQITLTVETAA
                     NRMLAPLAEQDLEEQSEDASDATPQETPGPDAPQATAGPAGEATPSPSPTIGPTPDPT
                     WTPEPLPTQEAVASPTPAATPVLGPTPDPTWTPEPLSTPQTATEEKVAPVIGPTPSPN
                     WTPEPLRPEGE"
     gene            4536..>5295
                     /locus_tag="ACZPFK_01715"
     CDS             4536..>5295
                     /locus_tag="ACZPFK_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405057.1"
                     /translation="MKTAVQKLKNSRRAAILLTLWAVAAFPLLCQFFVEWVRLQSMRR
                     ALAYFAGHLGAALLGTAFLAGFISLFTLLTRRPWVGAALAGGLLFIGAYVNFYKLTYR
                     GDPVLPKDLAIAGDAAKVATELSIPPTFQMGVFFLFFSLSIFLLLPIRLPAAGGAREA
                     ALRLAGSFAAAVFCGAYLWGVLWNSGVQGAFGIDTMAFQPSESYEKSTFATGFLMYTG
                     AMSPRAPKGYGEAAVGQAAEALPQTEHDGQRRPDV"
     assembly_gap    5296..5305
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5306..6417
                     /locus_tag="ACZPFK_01720"
     CDS             <5306..6417
                     /locus_tag="ACZPFK_01720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1405058.1"
                     /translation="IVVMLESYYRLDNVQGAAYDTDLTENYDRYAAEGISGSYISDKY
                     SGGTEEMEFGALTGFSTSLLPTGSIPYVEYVDGEFPCYPRFLKEQGYKTIALHPYDPT
                     IYSRNRAYPAMGFDEFYSQDDFDDPEKHGAYIDDVETAKKLIELYEQATADGGNVFLH
                     TVTMQNHIPNDPQEYAEEYKVNAELPGMPQYYVNSLESVATGLRDADRAIGLLCDYFA
                     GCDRDVVILFFGDHQTAIGDQNGRELLDELESFQALSEEEQLKAKHNAPYLMWANFEA
                     KEAESAGAMPPYQLLATMLSRYDVLRPAWFDFLAQSQTVMKGLNLGEVIGPDGSFAGP
                     VGEASQEQKDLLEKQKLLQYDAMFGQGFAHEEMYG"
     gene            6469..6663
                     /locus_tag="ACZPFK_01725"
     CDS             6469..6663
                     /locus_tag="ACZPFK_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405059.1"
                     /translation="MYPFLSTLAVLLGTALLAAAGTAKKKSGRAAAALCAAGALLTVA
                     GLTALFLLLTGRVALPLAGR"
     gene            complement(6849..>7496)
                     /locus_tag="ACZPFK_01730"
     CDS             complement(6849..>7496)
                     /locus_tag="ACZPFK_01730"
                     /inference="COORDINATES: protein motif:HMM:NF046303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6994 family protein"
                     /protein_id="HIR1405060.1"
                     /translation="TLRRYHQLLWSRQLPNGEFMNLKMGTGRNYLIWKDFCFGSDSIT
                     ASFRYQRYRTMLRQVSDALPNYRLFMESFIKKSYTIGGTIIFPKRRGNINQSRGCNNH
                     IKDRWDLTLDCIRKYYNNEKSPLYEVLQKDKDFFDLFLNFKGYIDYFFLQDCVAPDYK
                     TVHFWIAAESPHRTPLPQTVEEYLQWISQQINFIEKRNARIQAFCSCNKSSLNPG"
BASE COUNT         1536 a         2214 c         2444 g         1292 t
ORIGIN      
        1 gatgaagaaa aagataacgg cgctgatttt ggccgcagcc atggtcgcgt cgatctttgc
       61 gggctgcggc tccgggcccg cgcccggcga gagctcgtct caaaacatcg gggccggggc
      121 aggcgagacg ctgatcttga gcgagccagg caattataca gaaaaaaagg aatacggcga
      181 tgtggtgctc aaggccgacg gcattgtgct ggaaaatgcg cttatccacg gctcgctcac
      241 cgtggataaa acggtgggag aaggggacgc gaccctgcgc ggctgcacgg tggagggcga
      301 cgtctatctg tacggcggcg ggtcccacag cgtccatttt gaaaactgta tcttaaatac
      361 cctcatcgcg gaaaagaaag gcctgcgggt cgtgctggac agccgcaccc agacgggcgc
      421 ggtgagcgtc cgcgccagcg ctcggttcga gatttccgcc gccgtggagc gggtgttgat
      481 cgagacaggc gccgctgaca gcgaattgct gttcaccgag gaggcccagg ccagcaaggt
      541 gaccctgaac gcggcggcgc ggctggagca gaattgcccg ctcgaggagc tgaacgtggg
      601 cagaaaagcc tcgggcagca gcctgctcct ctccgcaaag acggaaaagg tggtcctcag
      661 cggcgcttgt tccgtggagg tgcaggccga cgtcggcgag ctggtgctca cgggcaacgc
      721 ctccggcagt gaggtcgcag tggcgggggg cgcgcaggtg gacaccctcg ccaccagtgt
      781 gcccctgacc ctgagcgggg agggcaccgt gaaacgggcc atctcggatg aaaaggcaaa
      841 gctgggcggt gacttggtcc ccgccacggt gaaagaaaaa gaggccccgg tgctggaggc
      901 ggacgggacg gcgagcgcga acctggcaac gcccaccaag gcgcccacgg cgacggcggc
      961 gccgaccaaa aagcctgtgc cgatgcccgt gcccacaccc tggaacccgg gcacggtgac
     1021 aacgccgacc gcggcgccca ccgcgaccgc gacgccggcg gtcacctcca cgcccacacc
     1081 ttctcccgcg ccgaccccga cccccacgcc gaccgcaacg ccggtgctgg tgtgggacgg
     1141 cacggcggac accagctggt acacgggcga cgcgcagagc tatgtgctgc agagcgcgga
     1201 acagctggcc ggtctggccg acctggtaaa cggcggcaac gattttgccg gggtcaccgt
     1261 cacgctgggg gtgagcctgg acctggacgg ccgcgagtgg acccccatcg gcagaagcag
     1321 ccgcagcggc ggtggcctgg cggcgggcag catgccgttt gcgggcgtct ttgacggcaa
     1381 tggcaaaacc atcgccggcc tggcgatcag cgccacaagc ggtgctgacg cgggcgtggg
     1441 cctgttcggc gcagtggccg gcagcggcgc ggccgtaaaa ggcctcaccc tgacggatgt
     1501 gaaagtggag gttgcttcca gcaaagcgct gggcgcggtg accggcctgc tcaccgacgg
     1561 ggcgatggtc tccgactgcc atgtaaaggg cggcacggtt tccggcacac agggggtcgg
     1621 cggcatcgtc ggccgcgggc tcaagaccgg tgtggtggag aactgctcca atgcggctgt
     1681 ggtcacggcc tccagcgcca acgcgggcgg catcgcgggc gcgatgtact acgaccagga
     1741 ccctgcgggc gcgggcgacc gggctccgtt cgcggtgcgc ggctgtgaga acagaggcgc
     1801 ggtaaaaggg cagtcggtgg cggtgggcgg cattgtcggc ctgtccacta cctgcgatat
     1861 cagcaattgc accaacagcg gcacggtgac cggcgggggc accagcgtcg gcggcatcgc
     1921 cggcgagcag aaattcggcg gtacggtttc cggctgcacc aataaggcgg ccgccacggt
     1981 cagcggcaat caatatgtgg gcggcatcgt gggctgggtc cgctacaatg gcgcgggcgg
     2041 tacctacaac accagccttt cggtcacagt aaaggattgc gtcaacgagg cgccggtaga
     2101 gggcacaaac tgtgtggccg gcatcgtggg cgatatctac tggagctgca ccctcacagg
     2161 ctgcaccaac agcggcgcga ttacctccac cggcatgggc gcgggggtcg cggtggagca
     2221 gagcctgtcc gcaggcggcg ggaaagacga caattactcg acggcgcgcg ccaaccgggt
     2281 ggaggtctcg ggctgcgtca acagcggcgc ggtcaacacg tcgcccaggg cggaaatcct
     2341 ctatgtcaac agcagcgacg gcgacggctg cacggtggtg gtggacggcg taaagctggt
     2401 ggacggcgac aagatccttg ccatcacgga tgggaccaac atctacggca cgctcgccga
     2461 agcggtggcg ggcgcaccgg acggcgcgac catcagcttg cccgagggcc ggtatgacga
     2521 gacggtcacc accgcaaaga cgctgaccat ccggggcagc ggcaccggca agaccgtcct
     2581 tgcaccctct gcctccgggg cgcgggccat tgtggtggac cggggctgta ccagcacgac
     2641 ggcagccgca gcggcggccg cgggcagttt gacactggaa catctgtcga tggagctgcc
     2701 cgcccagggg cggggcgtcc tggtggaaaa taccagcaac tataccgtgc ggctgaaaga
     2761 cgtcgacgcc tccacaaagg atacgggcac ggctttcctc tacctcaggg gagaggtcgt
     2821 cggcggcgaa aaacgggtcc agagagacct tttcgtggaa gcagaggggt tccgtctcaa
     2881 cgccgcgggc gatttgacca tcgaccgcat cttttcggct ttctacttcg gcaacctcac
     2941 cggcggcggc ctgcggctgc aaaactgtga tgtgcgcggc gcgcgctatg cggtcaacct
     3001 gcagcagtcc gcagggctgg cggtcgaggt cacggcctcg accgtgcagg gctggacaac
     3061 gctcaacgcg atcagcaaca actgcacctt tgaggtgaat ggcagcactt tgatcggcta
     3121 caattccatg actggtggca gcaacaacta cagcgtgctg gtcatcgacg gcgggcagat
     3181 catcggggta gacccggtgc agggctatac caatacgctg catgtcgcgg gctctaccct
     3241 gcgcgccgtg cgggcgcccg gctgttgtgt ggaaaaatat ctcagcatcc agtacagtgc
     3301 cacccaaaac caggccgtct ttgagaacgt cgctttcgag gcggaaaacg atcctctggg
     3361 cggaggaagc ggcttggacg tcggccccgg cgaccggctg gcggtgaacg gcgtcgaggt
     3421 gtcaaatgcg gcgccgcagt tcaccaagga ggacgacggc gattactggc tgctgaaggt
     3481 ctcttcagcc ggcgccgtga caggcgagtg gtacgacgac gggaccaagg cgaccatttc
     3541 caccgatacg gcgctgcaca ttttgaagac agaggccgag agcagaaagg tgtggggcta
     3601 tttcagcgct gtcacggagg ccgcgggcca aaaatgcagc gtcacctgcg acctggatat
     3661 ccggcagatc gcagagacca acatcccggt ggagggaatc gcttttacag gcgaaagcgg
     3721 tgatgcggcc acgctggctg tggacgaggc gcggcggttc agcgttgtcc tgcagccggc
     3781 agataccacc caccgaaccg tgacctggac cagcagcgac gagagcgtcg ccaccgtggc
     3841 cgctgacccg caagacccgg cgagtgcgca ggtgactgcg ctgacagcgg ggcagacggt
     3901 gttgagcgca accgcaggcg gcgtcaccgc acagatcacc ctcacggtgg agacggcggc
     3961 aaacaggatg cttgcccccc tggccgagca ggacctggaa gaacagtcag aggacgcgtc
     4021 cgacgccacg ccgcaggaga cccccggccc ggatgcgccc caggccacgg caggcccggc
     4081 gggagaggcg acaccctcgc cctcgcccac catcggccca acgccggacc cgacctggac
     4141 gccggagccg ctgcccacgc aggaggccgt cgcctccccc acacctgcgg cgacgccggt
     4201 cctggggccg acgccggacc cgacctggac gcccgagccg ctgtccacgc cgcagacggc
     4261 cacggaggaa aaagtcgccc cggtgatcgg cccgacgccg tcgcccaatt ggacgcccga
     4321 gccgctgcgg ccggagggag aatagaaaaa gcgatcaaag caggccatgg gcccggtgct
     4381 taccgggccc atggttattt ggacagagat ttttcagaat aaaattttga aaaaatgaac
     4441 aaatttgaaa gaaaaaaacc ggcagccgcg ttctataagt ggcaagcaaa tataaacgcc
     4501 cccgggccgc cgggggtgga agggactttt ttatcttgaa aacagccgtg caaaaactga
     4561 aaaacagccg ccgcgcggcg atcctgctca ccctgtgggc ggtggcggcc tttccgctgc
     4621 tgtgtcagtt ctttgtggag tgggtgcggc tgcagagcat gcgccgcgcc ctggcctatt
     4681 ttgcgggcca tctgggggcc gcgctgttgg gcacggcttt tttggccggc ttcatctctc
     4741 tgttcacgct gctcacccgt cgcccctggg tcggcgcggc cctggcggga gggctgttgt
     4801 tcatcggcgc ctacgtcaat ttttacaagc tcacctaccg cggcgacccg gtgctgccca
     4861 aggatttggc catcgccggc gacgcggcaa aggtggcaac cgagctgtcc atcccgccca
     4921 cctttcagat gggggtgttc ttcctcttct tttccctgag catttttctg ctgctgccga
     4981 tccgtctgcc ggcggccggc ggcgcaagag aggcggcgct gcggctggcc gggtcgtttg
     5041 ccgcggcggt gttctgcggc gcttatctgt ggggcgtgct ctggaacagc ggcgtgcagg
     5101 gcgctttcgg gatcgacacc atggcctttc agccctctga gagctatgaa aaaagcactt
     5161 tcgccaccgg ctttttgatg tacacgggcg ccatgtcccc gcgcgccccc aaagggtatg
     5221 gggaggcggc cgtcgggcag gcggcggaag cgctgccgca gacggagcac gacggccagc
     5281 gccgccccga cgtgannnnn nnnnntgatc gtggtgatgc tggagagcta ttaccggctg
     5341 gacaatgtgc agggggccgc ctatgacacc gacctgaccg aaaattacga ccgctatgcg
     5401 gccgagggca tctcgggcag ctatatctcg gacaaatact ccggcggcac cgaggagatg
     5461 gagttcggcg cgctgaccgg cttttccacc agcctgctgc ccaccggcag catcccctat
     5521 gtggaatatg tggatggaga gttcccctgt tatccgcgtt ttttgaaaga gcagggctac
     5581 aagaccatcg cgctgcaccc ctatgatccc accatctaca gccgcaatag ggcgtacccg
     5641 gccatgggct ttgacgagtt ttactctcag gacgattttg acgacccgga aaagcacggc
     5701 gcctacatcg acgatgtgga gaccgccaaa aagctgatcg agctctacga gcaggccacc
     5761 gccgacggcg gcaatgtctt tttgcacacg gtgaccatgc agaaccacat ccccaacgac
     5821 ccgcaggaat acgccgagga gtacaaggtg aacgccgagc tgcccggcat gccgcagtac
     5881 tatgtgaata gcctggagag cgtcgccacc ggcctgcggg acgcggaccg ggccatcggg
     5941 ctgctctgcg attattttgc cggctgtgac cgggacgtgg tgatcctgtt tttcggcgat
     6001 caccagactg ccatcggcga ccagaacggc cgggagctgc tggacgagct cgagtccttt
     6061 caggcgctca gcgaggagga gcagctcaag gccaagcaca acgcccctta tctgatgtgg
     6121 gccaactttg aggccaagga ggcggagagc gcgggggcca tgccgcccta ccagctgctg
     6181 gcgaccatgc tgtcccgcta tgacgtgctg cggcccgcct ggttcgattt tctggcccag
     6241 agccagaccg tgatgaaagg gctcaacctg ggcgaggtga tcggcccgga cggcagcttt
     6301 gccgggccgg taggcgaggc ctcccaggag cagaaagacc tgttggaaaa acagaagctg
     6361 ctgcagtatg acgccatgtt cggccagggg ttcgcgcacg aggagatgta cggctgaccc
     6421 caatgtgccc gccgcgggag gccgaaaaag gcaggaagag acgcatccat gtatccattc
     6481 ttgagtaccc tggccgtgct gctgggcacc gcgctgcttg cggccgccgg gacggcgaag
     6541 aaaaagagcg gccgggccgc cgcggccctg tgcgcggcgg gggcgctgct gacggtcgcg
     6601 gggttgacgg cgctttttct gctgctgacc ggccgcgtgg ccctgccgct ggcggggaga
     6661 tagaggctgc gaaaagagga gagagttatc cacaggggaa ctcgggagac ggagaactcg
     6721 aacaaaagag cttttacttt aaaaaagaaa actctcgttg caataccgcg gaatatcata
     6781 aaaaagagca aaagccctag accatcatcc gatgatctag ggcttttgct ttgtcacaca
     6841 attccagtct aaccgggatt taaagaagat ttattgcagc tacaaaatgc ttgaatcctt
     6901 gcatttctct tttcaataaa atttatttgt tgagagatcc attgaagata ttcctctaca
     6961 gtctgtggta atggagtgcg atgagggctt tcggctgcta tccagaagtg tacggtctta
     7021 taatctgggg cgacgcaatc ttgaagaaaa aaatagtcta tatagccctt gaaattcaaa
     7081 aaaaggtcaa aaaaatcttt atctttttgt aaaacttcat ataaagggct tttctcatta
     7141 ttataatatt ttcgaataca gtccaaggtt aaatcccatc tatccttaat atgattgttg
     7201 cagcctcttg attgattgat gttacctcgc cgtttcggga aaatgatggt tcctccgatt
     7261 gtgtaagact ttttaataaa agattccata aagagtcgat aatttggtag agcatcgctt
     7321 acttgtcgga gcatcgttcg gtagcgctgg tatcgaaaac tggcagtgat agaatcactg
     7381 ccaaagcaaa aatctttcca tattaaataa tttctgccag tccccatctt tagattcatg
     7441 aattccccgt taggtaactg tcggctccac aaaagttggt gatatctccg caatgt
//
LOCUS       DBMDYA010000039         7250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2673_length_7250_cov_1.548049, whole genome shotgun sequence.
ACCESSION   DBMDYA010000039 DBMDYA010000000
VERSION     DBMDYA010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_7250_cov_1.548049"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            138..842
                     /locus_tag="ACZPFK_01735"
     CDS             138..842
                     /locus_tag="ACZPFK_01735"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR1405061.1"
                     /translation="MAYEALREQVYEANMLLPRHGLVTFTWGNASQADRAAGVVAIKP
                     SGVEYEALTPRDMVVLDLESGRVVEGGLNPSSDTRTHLELYRAFPDLGGVVHTHSPSA
                     VAWAQAGRDIPCYGTTHADYFYGPVPCARQLTQAEIDEDYEKNTGAVIVETFRRRGLD
                     PMHLPGVICSSHGPFTWGKDAAQAVYHAVVLEEVAKMAILTREVDPSAAPAPQRVQDK
                     HFLRKHGPAAYYGQKL"
     gene            857..1705
                     /locus_tag="ACZPFK_01740"
     CDS             857..1705
                     /locus_tag="ACZPFK_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIR1405062.1"
                     /translation="MERYRLGLYEKSMPGSLSLSQKLEETARAGFDYMELSIDETDEK
                     LARLDWTAGEAKALVDAMYAVGVPIHSICLSGHRRYPLGHPDEAVRRRSLQIMEKALA
                     LASRLSVRVIQLAGYDVYYEPGTSDTRRWFEENLDQAALLAARYGVTLAFETMETPFM
                     NTVEKAMAQVRRVNSPWLQVYPDVGNLTNAARAAGGSVEEDLRLGRGHLAAVHLKETV
                     PGVFREVPFGAGHVDFVSAARTALELGVRAFVGEFWYTGGDAWRRDLAQADGFLRAKL
                     RAAQTG"
     gene            1845..2936
                     /locus_tag="ACZPFK_01745"
     CDS             1845..2936
                     /locus_tag="ACZPFK_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1405063.1"
                     /translation="MKKLLALILAGAMALSMAACGGSDSSSGSATVSTPGSGASGAAS
                     TAGGDHTFETDYWATLTPDDPVTWDAPNNYKRVGMVVPDGTNEFYVNMCTTAEEIFKK
                     QGYDFSWTGATDSETAINAIDTWVTQGVDALILLVQDTACENAALKAMEQGVLVVLGS
                     ATFSSYHVWCCQDYYNIGYNIAKMAAPDMKEKFGDDASYIVLGSTKAEFQNQKTNGLI
                     DGMKELYPNGTCYEVIDSSNYQDDVETLLTQHPEISVVVSWHATMTLQAISALDTLGL
                     NDPDNFAVYGSQMTEQSLIEIKDPESCYVGDTWMGDQGRQYANVVLTLLNGGTVNHYD
                     YAPDFEVTAENAETYYEDYYKEFGDTYVY"
     gene            3033..4517
                     /locus_tag="ACZPFK_01750"
     CDS             3033..4517
                     /locus_tag="ACZPFK_01750"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405064.1"
                     /translation="MGRTILSTQNLTKLYPAVRANSDISIEIEEGEVHAIVGENGAGK
                     STLIKMIAGAHAPTSGTITIDGKVYHSLKPEQARQLGIEVIYQEFNLIPGMTCAENIY
                     LNKKTSKGPVVNAKERSRMARELFESLGVDIDPDRPVGSYSPGYMQIVEISKAIGQNV
                     RILIMDEPTAPLTVAEVELFFNIIRGLKEKGITIIYISHRLEEIFEIADRVSVFRDGR
                     YIDTKKTSETDRQELINKMVGRELTESYPKRAVEIGEETLRAENLCGNGVRNINFTAH
                     RGEILGFAGLVGAGRTELMSVIYGAAKQEGGALYLHGKSIRMKMPSDAIAQGIGLIPE
                     DRKSMGVFQQWSISWNTVIMCLKKVSKNLMISTKAERELSEKYIRELGIRTPSSEQKV
                     MNLSGGNQQKVVLAKTMAAETEILIFDEPTRGIDVGAKQEIYALMNRLAADGHTILMV
                     SSDLPELLGMSDRVVVLCEGEQAGIVEKKDFSQEHILDLASGNK"
     gene            4536..5492
                     /locus_tag="ACZPFK_01755"
     CDS             4536..5492
                     /locus_tag="ACZPFK_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405065.1"
                     /translation="MAADKFFKFYKKYGLFVVFAIIFIIFSIVAEGFLNPRNLINILK
                     QASIYGVMCVGVTIVMVSGGMDMSVGGQIACSGLVMAALMKQGVPFIFAILAGVACCA
                     LVGYINGMFIATFNCAPMVITLCLMLILNGLAVVVTNGIPIYDFPKSFYYVGQGFIGG
                     WFPVSIIVFVLAVGVGYFLLNKSYFGRKLYAIGGNKEAARLAGINIFKTRILSYVINS
                     VFIGLGAWVLLSKTGSASPTAGANYQFDCLTACMLGGVTFGGGSGNVLTTALGVLIIS
                     MIGNGLLLMGYDGNFQAIIKGLILLLAMTMDAYQKIRKAKTT"
     gene            5532..6413
                     /locus_tag="ACZPFK_01760"
     CDS             5532..6413
                     /locus_tag="ACZPFK_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405066.1"
                     /translation="MRLDAMPPEYRAKFMAAPHTFWLEPFRVEGNLYFIGNKEIACYL
                     IDTGEGLIILDTGYENCSGQFFNSIWSLGFDPRDVRMIFHTHNHQDHIASTNLLVQLS
                     GARTYMSETDGRAMVESPAGPLCPEALKFVPDVYTHDGDEYTLGSTTVRCIDTPGHTR
                     GCQSFFVNVTGADGTRYTAALHGGVGINTLHREFMEKTGYPQAKEDFFGHIQRIIDWP
                     VDIYLSSHTFQNYTEEKLAKRRADPAGPNPFVDPGEWHSILTTAWEMGHQLVEDEKNG
                     LVPEWQRQTYADLKEEQ"
     gene            complement(6477..6905)
                     /locus_tag="ACZPFK_01765"
     CDS             complement(6477..6905)
                     /locus_tag="ACZPFK_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1405067.1"
                     /translation="MDTLDKQILALLEQNARMSVKEIAAGVALTPPAVSQRIRRLEQE
                     GILQGYTTVIDLAKAGRSIRALISLSVPPAAREDFLHLVAQQPSVLQCHHVTGDYSYI
                     LSVATRDMDSLERLINRFQKIGQTSTQIILSSTEASVKLL"
     gene            6996..>7250
                     /locus_tag="ACZPFK_01770"
     CDS             6996..>7250
                     /locus_tag="ACZPFK_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405068.1"
                     /translation="MLIILKQNQNVKREKESQTKKTLDFYPLLNMIRGVADRSHNLLI
                     LSHPSIKTKNSRHSRAAVFLCFKQMPPRGGESRDVGEKTGH"
BASE COUNT         1591 a         2090 c         2241 g         1328 t
ORIGIN      
        1 cgctttgaga cccagaccgt ggacgagggt gtgggcccca gcaacgccgt catctacaac
       61 ggaccgtccg gcgattgcat ttttgccgcc gaccgggagg ccgcgcaggc tgcggtctat
      121 tttgcggagg gatgagtatg gcatatgaag cgttgagaga acaggtctac gaagccaata
      181 tgctgctgcc gcgccatggt ctggttacct ttacctgggg caacgcctcg caggcggacc
      241 gcgcggccgg cgtcgtcgcc atcaagccgt ccggggtgga gtatgaggcc ctgacgccgc
      301 gggatatggt ggtgctggac ctggagagcg gccgggtggt ggagggcggg ctgaacccct
      361 cctccgacac caggacccat ctcgagctgt accgggcctt tcccgacctg ggcggcgtgg
      421 tgcacaccca cagccccagc gccgtcgcct gggcccaggc cgggcgggac atcccctgct
      481 acggcaccac ccacgccgac tatttctacg gcccggtgcc ctgcgcgcgc cagctcaccc
      541 aggccgagat cgacgaggac tatgaaaaga acaccggcgc cgtgatcgtg gagacgttcc
      601 gccggcgcgg gctggacccg atgcacctgc ccggcgtgat ctgcagcagc cacggcccct
      661 ttacctgggg caaagacgcc gcccaggcgg tgtatcacgc ggtggtgctg gaggaggtgg
      721 ccaaaatggc catcctcacc cgggaggtgg accccagcgc cgccccggcg ccccagcggg
      781 tgcaggacaa gcactttttg cgcaagcacg gccccgccgc ctactacgga cagaaattat
      841 gacggggggc ggggagatgg agcgttaccg gctggggctc tatgaaaaaa gtatgccggg
      901 cagcctctcg ctgtcgcaaa agctggagga gacggctcgg gcgggcttcg actatatgga
      961 gctgtcgata gacgagaccg acgagaagct ggcgcggctg gactggacgg cgggggaggc
     1021 caaggccctg gtggacgcca tgtacgcggt gggggtgccc atccactcca tctgcctgtc
     1081 cgggcaccgc aggtatccgc tgggccaccc ggacgaagcg gtgcggcggc gcagcctgca
     1141 gatcatggaa aaggcgctgg cgctggcctc ccgcctctcg gtgcgggtca tccagctggc
     1201 cggatacgac gtctattacg agcccggcac cagtgacacc cgacgctggt ttgaggaaaa
     1261 tctcgaccag gcggcgctgc tggcggcaag gtacggggtg acccttgcct ttgagaccat
     1321 ggagacccct ttcatgaaca cggtcgaaaa ggccatggcc caggtgcggc gggtaaacag
     1381 cccctggctg caggtctacc cggacgtggg caacctcacc aacgcggcga gggccgcggg
     1441 cggcagcgtg gaggaggatc tgcggctcgg ccgcggccat ctggcggcgg tgcatctcaa
     1501 agagacggtg cccggcgtat ttcgggaggt gcccttcggg gcgggccatg tggactttgt
     1561 ctccgccgcc cgcacggcgc tggagctggg cgtgcgcgcc tttgtgggcg agttctggta
     1621 taccggcggc gacgcctggc ggcgggacct ggcgcaggcg gacggttttt tgagggcaaa
     1681 gctgcgggcg gcgcaaaccg gttgacagaa attggatttg gccccgcacc ccgcggcgcg
     1741 ataggatagg gatagaagat cagtggccaa cggcggcaaa agcgccgtga ctatacagca
     1801 tgtgaaaatg aacggaaccc atcacagaaa caggaggaaa gaaaatgaaa aagctattag
     1861 cactgatcct ggcgggcgcg atggcgctct cgatggccgc ctgcggcggc tcggattcca
     1921 gttccggcag cgcgacggtt tccacgcccg gttcaggcgc ttccggcgcg gccagcacgg
     1981 cgggcggaga tcacaccttt gaaaccgatt actgggccac cctgacgccc gacgatcccg
     2041 tcacctggga cgctcccaac aattacaagc gggtgggcat ggtcgtgccg gacggcacca
     2101 acgagttcta tgtcaatatg tgcaccacgg cggaggagat cttcaaaaaa caggggtatg
     2161 acttttcctg gacgggcgca accgacagcg agaccgccat caacgccatc gacacctggg
     2221 tgacccaggg cgtggacgcg ctgatcctgc tggttcagga taccgcctgc gagaacgcgg
     2281 cgctcaaggc catggagcag ggcgtgctgg tggtgctggg ctcggcgact ttctccagct
     2341 accacgtctg gtgctgccag gactattaca acatcggcta taatatcgcg aaaatggctg
     2401 ccccggatat gaaagaaaaa tttggcgacg acgcctctta catcgttctc ggcagcacca
     2461 aggccgagtt ccagaaccag aagaccaacg gcctgatcga cggcatgaaa gagctctatc
     2521 ccaacggcac ctgctacgag gtcatcgaca gttcgaacta tcaggacgac gtggagaccc
     2581 tgttgaccca gcaccccgag atcagcgttg tcgtgagctg gcacgccacc atgaccctgc
     2641 aggccatctc ggcgctggac accctgggtc tcaacgaccc ggataacttt gcggtctacg
     2701 gctctcagat gaccgagcag tcgctgatcg agatcaagga cccggagagc tgctacgtgg
     2761 gcgatacctg gatgggcgac cagggccgcc agtatgccaa cgtggtgctg acactgctga
     2821 acggcggcac cgtcaaccat tacgactacg cccccgactt tgaggtcacc gcagagaacg
     2881 cagagaccta ttacgaggac tattacaaag agttcggcga cacctatgtc tattaaaaaa
     2941 gtgcatcagg cgtaaaaaca gcgggcggag gcaggctcat acaaccctgc cttcgtccgc
     3001 aaaatttacc ctccgaaagg agaaagaacg gaatgggaag aaccatcctc agtacccaga
     3061 acctgacaaa attgtatccg gctgtgcgcg ccaacagcga tatttccatt gagatcgaag
     3121 agggcgaagt gcacgccatc gtcggtgaga acggggccgg caaatccacg ctgatcaaaa
     3181 tgatcgcggg cgcccacgcc cccacctccg gcacgatcac catcgacggc aaggtctatc
     3241 attccctcaa acccgaacag gcccggcagc tggggataga ggttatctat caggagttca
     3301 acctgatccc cggcatgacc tgcgcggaga atatctatct caataaaaag acgagcaagg
     3361 gccccgtggt caacgccaaa gagcgctcgc gcatggcgcg ggagctgttt gagagcctgg
     3421 gcgtcgacat cgaccccgac cgtcccgtgg gcagctattc gccgggctat atgcagatcg
     3481 tcgagatctc gaaagcaatc ggccagaatg tgcgcatcct gatcatggac gagcccaccg
     3541 ccccgctgac cgtggccgaa gtggagctgt tcttcaacat catccgcgga ctcaaggaaa
     3601 aggggatcac gatcatctat atctcccacc gcctggagga gatctttgag atcgcagaca
     3661 gggtttcggt cttccgcgac ggcaggtata tcgacactaa aaagaccagc gagaccgacc
     3721 ggcaggagct catcaacaag atggtggggc gtgagctcac cgagagctat cccaaacggg
     3781 cggtggagat cggggaggag acgctgcgag cggaaaacct ctgcggcaac ggggtgcgca
     3841 acatcaactt tactgcgcac cggggcgaaa tccttgggtt cgcgggcctg gtcggcgccg
     3901 gcagaaccga gctgatgagc gtgatctacg gcgccgccaa gcaggagggg ggcgcccttt
     3961 acctgcacgg caagtcgatc cggatgaaga tgccctccga cgccatcgcc caggggatcg
     4021 ggcttatccc cgaagaccga aaatccatgg gcgtgttcca gcagtggtcc atctcctgga
     4081 acacggtgat catgtgcctg aaaaaggtca gcaaaaacct gatgatcagc acaaaagcgg
     4141 agcgggaatt gtcagaaaaa tatatccggg agctgggcat ccgcaccccc tccagcgagc
     4201 agaaagtcat gaacctgtcg ggcggcaacc agcaaaaagt ggtgctggcc aaaacgatgg
     4261 cggcggagac ggagatcctg atttttgacg agccgacccg cggtatcgac gtgggggcaa
     4321 agcaggagat ttacgccctc atgaaccggc tggcagcgga cggccacacc atcctgatgg
     4381 tctcctccga tcttccggag ctgctcggga tgtccgacag ggtggtggtc ctctgtgagg
     4441 gcgaacaggc cggcattgtg gagaagaagg atttcagcca ggagcatatc ctcgatcttg
     4501 cttcgggcaa caaataaacc aaaaggggaa ggacaatggc tgcggataag tttttcaaat
     4561 tttacaaaaa gtatgggctc tttgtcgtgt ttgcgatcat ctttatcatt ttctccattg
     4621 ttgcggaggg ctttttgaac ccgcgcaatc tcatcaacat cttgaaacag gcgtctattt
     4681 acggcgtcat gtgcgtgggc gtcacgatcg tgatggtttc gggcggcatg gatatgtcgg
     4741 tcggcgggca gatcgcctgc agcggcctgg tgatggcggc gctcatgaag cagggcgtcc
     4801 cctttatctt cgcgatcctc gccggcgtcg cctgctgtgc tctggtgggc tatatcaacg
     4861 gcatgttcat cgccacgttc aactgcgcgc ccatggtgat cacgctctgc ctgatgctca
     4921 tcctcaacgg cctggcggtg gtggtcacca acggcatccc gatctatgac tttccaaaaa
     4981 gcttttacta tgtgggccag ggttttatcg gcggctggtt cccggtcagc atcatcgtct
     5041 ttgtgctcgc cgtaggcgtg ggatactttt tgctcaacaa atcctacttt gggcgcaagc
     5101 tgtacgccat cggcggcaac aaagaggcgg cgcgcctcgc gggcatcaat atcttcaaga
     5161 cgcgcatcct gtcctatgtg atcaacagcg tctttatcgg cctgggcgcg tgggtgctgc
     5221 tgtccaaaac cggcagcgcc tcccccacgg cgggcgccaa ctaccagttc gactgtctga
     5281 ccgcctgcat gctgggcggg gtcacgttcg gcggcggctc aggcaatgtc ctcaccaccg
     5341 cgctgggcgt tttgatcatc tcgatgatcg gcaacgggct tttgctcatg ggctatgacg
     5401 gaaacttcca ggccatcatc aagggcctca tcctgctgct cgctatgacc atggatgcct
     5461 atcagaagat ccgcaaggcc aagaccacat aaacatcgtc catcaacaag ggataaaggg
     5521 ggattttaca gatgcgttta gatgccatgc cgccggaata ccgggcaaaa tttatggccg
     5581 cgccccacac cttttggctg gaacctttcc gggtggaggg aaacctgtac ttcatcggta
     5641 ataaggagat cgcctgctac ctgatcgaca cgggagaggg gctgattatc ctggatacgg
     5701 gatatgaaaa ctgctcaggt cagtttttca actccatctg gtcgttgggg ttcgatccgc
     5761 gggatgtcag aatgatcttt cacacccaca accaccagga ccatatcgcc tcgacaaatc
     5821 tgctggtaca attgtcgggg gcgaggacct atatgagcga gaccgacggc agggcgatgg
     5881 tggaatcgcc cgccggcccc ctgtgcccgg aagccttgaa gtttgtgccg gacgtgtaca
     5941 cccatgacgg ggacgagtac accctgggca gcacgaccgt gcgctgtatc gacaccccgg
     6001 gccataccag gggatgccag tcgttctttg tcaatgtgac cggcgcggac gggacccggt
     6061 ataccgcggc cctgcacggc ggggtgggca tcaacaccct gcaccgggaa ttcatggaga
     6121 agacgggcta cccgcaggcc aaggaagact ttttcggcca tatccagcgg atcatcgact
     6181 ggccggtgga tatctatctc agcagccaca cctttcagaa ctacaccgag gaaaaactcg
     6241 ccaagcggcg cgccgacccg gctgggccga acccctttgt cgatcccggc gagtggcaca
     6301 gcatcctcac caccgcgtgg gagatgggac accagctggt agaggatgaa aagaacggcc
     6361 tggtgccgga gtggcagcgg cagacctatg ccgatttgaa agaggagcaa taagctgtaa
     6421 aaaagcagcc cgacccgcaa aaaactgcgg gccgggctgc ttttttctag aggaggctac
     6481 agcagcttca cgctggcctc cgtgctggag agaatgatct gggtgctggt ctggccgatt
     6541 ttttgaaagc ggttgatgag gcgctccagc gaatccatgt cgcgggtggc cacgctgagg
     6601 atatagctgt agtcgccggt gacgtgatgg cattgcagca ccgagggctg ctgggccacc
     6661 aggtggagaa aatcctcccg cgcagcgggc ggcaccgaga ggctgatcag ggcgcggatg
     6721 ctgcgccctg ctttggccag gtcgatgacg gtggtgtacc cctggaggat gccctcctgc
     6781 tccaggcggc ggatgcgctg ggagacggcg ggcggggtga gcgccacccc ggcggcgatc
     6841 tccttgacgc tcatgcgggc gttctgttcc agcagggcca ggatctgttt gtccagcgtg
     6901 tccatgacgg cgtcctccct caaagttttt acaaaataaa gttagcgaac cgatcaaaag
     6961 aaaaatattt taaaaacaaa agcaaaaacg atttcttgtt aattatatta aaacaaaatc
     7021 aaaatgtaaa gcgcgaaaaa gaaagccaaa ccaaaaaaac gcttgacttt tatccgcttt
     7081 tgaatatgat tagaggtgta gcagatagat cacacaatct attgatactt tcccacccct
     7141 ctataaaaac gaaaaacagc cgccattccc gggcggctgt ttttctttgt ttcaaacaga
     7201 tgccgccgcg tgggggagaa tcccgggatg tcggagaaaa aacggggcac
//
LOCUS       DBMDYA010000040         7206 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2680_length_7206_cov_1.665869, whole genome shotgun sequence.
ACCESSION   DBMDYA010000040 DBMDYA010000000
VERSION     DBMDYA010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_7206_cov_1.665869"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="ACZPFK_01775"
     CDS             complement(<1..304)
                     /locus_tag="ACZPFK_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1405069.1"
                     /translation="MDMFKNLNGKKLVLGLVHLLPMPGTPLYEEGNLEKMTEKAIQDC
                     KTLQNAGADGGLIQSVDVIYPSTDDTDYARVATLAMIAGRVRAAVGPDFKMGVQLMW"
     gene            complement(382..1350)
                     /locus_tag="ACZPFK_01780"
     CDS             complement(382..1350)
                     /locus_tag="ACZPFK_01780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405070.1"
                     /translation="MAIEYLACGNIMSDRIERQDGSFSEWNMGGPAFFALSGIRLWTK
                     ACKLVCRTGEDYASTYGRWMDANGVSRESVQVEAENVTRFTLKYNPDGSFSPTPHFSM
                     EHLGYLKTHPEEIDQACAGQAVKGIYMAHNLDPVIWGKLGEVKEKYGFKIMWEIEYAG
                     QIRKMLRLTRQQILDRARRVMEVADLWSINHNEAADLFQLPREDDEAIINELQKLPVE
                     FTFYRVGSRGSYAVTPTNAYFCEAVDPFGPSVDPTGCGNCSTGAAMYAYVSGEHPAMA
                     AVMANISSGFNAAQKGPYPLYTEEDMQLARRLAAERFEALGLTVPR"
     gene            complement(1351..2298)
                     /locus_tag="ACZPFK_01785"
     CDS             complement(1351..2298)
                     /locus_tag="ACZPFK_01785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405071.1"
                     /translation="MDYIAAGNVMLDTVRFADGSQSNREHIGGPSTFAYSGIRLWTDS
                     VLQCSNVGADYAELFLPWIEKNHIDTRGLKIKCDHCNHSFLVYNPDGTYGGDPEVERF
                     RSDWVQDFGYMKTSPEEIGEFTRRGGVKGVYLAQNVDYVFWEKLAAIKKRDGFKMMWE
                     IEGPSSYKRYLPDVLNAMKTTDIFSINIQEAQNLFEVEGEEACIAELQKMPVNVTLFR
                     VGKKGLYSVTPDKVYFVPSAPVDREVDPTGCGNTSTGSALYAYAEGYDPLMIGIMANV
                     ASAQNIRQFGVIPDFAAVRAQAFAQAKELYDAYPRGEGC"
     gene            complement(2359..3507)
                     /locus_tag="ACZPFK_01790"
     CDS             complement(2359..3507)
                     /locus_tag="ACZPFK_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1405072.1"
                     /translation="MKQNYDNPLRRQCFDLVRLAPAQFDLAREALLRLEPEKRLAGIR
                     RVVVTGCGDSYLAAAQAREVFKHYLKGTGCRMEAVRAIEAARYLPLEEDAAGTLLIAI
                     SASGGPARIAEILQRGKQHGCLTMALTNNGASRAGTTADLAYVTNTPEFPDRMPGLRS
                     YLASMMGLFVLAAVLGEVKSGKTGLCDGLRAALEAYNGRFADALEQMDDSAYAAAVAM
                     KDSKGFEVCADGPLFCCGEFIPAKYAEVSGDKCTVVDSENFWHVNSIMMPQNAYGTID
                     YAVSDEGNCARMAETISGQVEKLGRKVLVVADKPAAELGVTAPVMECILPVPDRQFRF
                     LLPLYAFVPGSLVAGYHAALIEEPYFRGGGKFFDPSINTLGTNGIRVL"
     gene            complement(3525..4640)
                     /locus_tag="ACZPFK_01795"
     CDS             complement(3525..4640)
                     /locus_tag="ACZPFK_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1405073.1"
                     /translation="MDKQNYYNTIGLQVESLFELAPIQVEKCFDEKKLSALLPLPEAA
                     ALKRLIVAGCGDSYSAAGAMAPVIRKLACVSCASPDPMELCSFYTDEDIRDGCKPEEV
                     LVVAISASGGSVRIVEMLERARAAGVRSMLITNNPESKGAKAAELVYCVETPELCNTP
                     GLRSYFASLTALAALASYIGGSKGVAEPAVARQAILDYVSACQPRYQEIDDQMFELAQ
                     RWSGLEKFEIVGDESEFFSAQFVEEKFIECASVHASHADSEDWCHINFFLREPETVGT
                     IFQVNAAAPDLDRIAESVRVSVAIGRPTLVVTDADCDFAEGAAVCRLPKAPECAPWAL
                     LMMDHAPGSLLAAYCAALAGKLFFSNRYDFRTRTWKM"
     gene            complement(4704..5705)
                     /locus_tag="ACZPFK_01800"
     CDS             complement(4704..5705)
                     /locus_tag="ACZPFK_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405074.1"
                     /translation="MLEFVSNFIYSLIRIATPLIFVSICSTISQQAGLLNMAAEAMML
                     TASLGGVLFSAWTQNVWLGILGGALTAVLIALFLCFATFIMKVDLYLMSISLNMALLG
                     GTVFVVYLVTGTKATTAGVVKSLALGNVDIPFIKDIPFIGSILSGHNVFTYFSIFMVF
                     AVWFLLFRTKIGLRMRAVGQNPQAAESVGINPRKIYTLAFALAAFVGSFGGMYLSMGY
                     QNFFIRNITANRGFVGLAAATIANGMPIGSMLMSFVFGLAYAVTNYLKPYVVDQYFLT
                     ALPFIMIVLLYFAMSFWRSKQADRLLKMNRQRLEEEREKQLWEEAQAKKSAPGSGES"
     gene            complement(5698..6780)
                     /locus_tag="ACZPFK_01805"
     CDS             complement(5698..6780)
                     /locus_tag="ACZPFK_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405075.1"
                     /translation="MFEFLRVFLGIVIAFLACIVIIVLISEESAGDAVYNFVVGPFMS
                     PRRFGQVLARYAPYLLTGCGMCFIYACGRFSLISEGIINIAPIFALMVMFRTPLMTNL
                     PQFVNLIIIIVVCAIAGGAFAMVPAFGREKLGANEMVTSTIMNYMCLYLALWLLRSTV
                     ADRGQSFLTSPIFPDNMRFTRYWGDTNFTSAIWVAIIGVIVACILFYRTPLGAKIRMC
                     GSNLSFATYSGINAKKMMYLAQLIGGLFAGVAAAVDCFGTYNSYYYQILTNVGMDGLL
                     CAVMARKQPLFVPLTAFVLAYIRTAASVLNANTNIPIELVTMLQAVIVLFVAAEEFLG
                     KTKRRVIFNISREEEAVKVKEAKSNA"
     gene            complement(6804..>7206)
                     /locus_tag="ACZPFK_01810"
     CDS             complement(6804..>7206)
                     /locus_tag="ACZPFK_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405076.1"
                     /translation="DHCIEEFEIACTGGDEAIHLLSGGNIQKVIIAREFSSGANLIVA
                     NQPTRGIDVGTTDLIRRLLVKYTREKKIGALLISSDLNEILEVSDRLLVMKDGQIVAQ
                     FNDVKSVTAEQLGEYMLGVKSMTPEEMGDLF"
BASE COUNT         1334 a         2181 c         2363 g         1328 t
ORIGIN      
        1 tccacatcag ctgaacgccc atcttgaagt cggggcccac ggcggcgcgc actcggccgg
       61 cgatcatggc cagggtggcg acccgcgcgt agtcggtgtc gtcggtggag gggtagatga
      121 cgtccaccga ctggatcagg ccgccgtccg cgcccgcgtt ctgcagcgtc ttgcagtcct
      181 ggatggcttt ctcggtcatc ttctccagat taccctcctc atagagcggg gtgcccggca
      241 tgggcagcag atggaccagg cccaggacca gcttttttcc gttgaggttt ttgaacatat
      301 ccataatcag gtctcctttg tgatgtgtgt gatattttga ttttgcctgc gggcgggccc
      361 gcaggggcga aaaaagcggg ctcagcgggg gacggtgagc cccagcgctt caaaccgctc
      421 ggcggcgagc cgccgggcca gctgcatgtc ctcctccgtg tagagggggt agggcccttt
      481 ctgcgcggcg ttgaagccgg aggagatgtt ggccatgacc gcggccatgg cggggtgctc
      541 ccccgagacg taggcgtaca tggcagcccc ggtggagcag ttgccgcagc cggtgggatc
      601 caccgagggg ccaaaggggt ccaccgcctc gcagaaatag gcgttggtgg gggtgacggc
      661 gtaagagccg cggctgccca cccggtaaaa ggtgaactcg acgggcagct tttgcagctc
      721 gttgatgatg gcctcgtcgt cctcgcgggg cagctggaac aggtccgccg cctcgttgtg
      781 gttgatggac caaaggtcgg ccacctccat cacccggcgc gccctgtcca gaatctgctg
      841 ccgggtgagg cgcagcatct tgcggatctg gcccgcgtac tcgatctccc acatgatctt
      901 gaagccgtat ttctccttga cctcgcccag cttgccccag atgacggggt ccaggttgtg
      961 ggccatatag atgcctttca ccgcctggcc ggcgcaggcc tggtcgatct cctcggggtg
     1021 ggtcttcagg tagcccagat gctccatgga aaaatggggc gtgggggaga aagagccgtc
     1081 ggggttgtac ttcagcgtga agcgggtcac gttctccgcc tccacctgca ccgactcccg
     1141 cgacacgccg ttggcgtcca tccagcggcc gtaggtggag gcgtagtcct cgccggtccg
     1201 gcagaccagc ttgcaggcct tggtccacag ccggatgccc gagagggcga aaaaggcggg
     1261 gccgcccatg ttccactcag aaaagctgcc gtcctgccgc tcgatgcggt cggacatgat
     1321 attgccgcag gccaggtatt ctatcgccac tcagcatccc tccccgcgcg gataagcgtc
     1381 gtacagctct tttgcctggg cgaaagcctg ggcgcgcacg gcggcaaagt cggggatgac
     1441 gccgaactgg cggatgttct gggccgaggc gacgttggcc atgatcccga tcatcagggg
     1501 atcatagccc tccgcgtagg cgtacagcgc tgagccggtg gaggtgttgc cgcagccggt
     1561 ggggtcgacc tcccggtcga ccggagcgga ggggacaaag tagaccttgt cgggggtcac
     1621 cgagtagagc ccttttttgc ccacccggaa cagcgtgacg ttcacaggca ttttctgcag
     1681 ctcggcgatg caggcctctt cgccctcgac ctcaaacagg ttctgcgcct cctggatatt
     1741 gatggagaag atatccgtcg tcttcatggc gttcagcacg tcgggcagat agcgcttgta
     1801 agagctggga ccctcgatct cccacatcat tttgaagccg tcccgttttt tgatggcggc
     1861 cagcttttcc cagaagacgt agtccacgtt ctgcgccaga tacacgccct tgaccccgcc
     1921 gcggcgggtg aactcgccga tctcctccgg cgaggtcttc atatagccga agtcctgcac
     1981 ccagtcgctg cgaaagcgct ccacctccgg gtcgccgccg taggtgccgt ccgggttgta
     2041 gacgagaaag gagtggttgc agtgatcgca ttttatcttg aggccccggg tgtcgatgtg
     2101 gttcttctcg atccagggca aaaacagttc cgcatagtct gcgcccacgt tgctgcactg
     2161 cagaacgctg tccgtccaaa ggcggatgcc gctgtaggca aaggtggacg ggccgccgat
     2221 gtgctcccgg ttggactggg agccgtccgc aaagcggacg gtgtcgagca tgacgttgcc
     2281 cgctgcaata tagtccatat acattctcct aacacgtgaa ttttgaggat gggcgggcgc
     2341 gggggcccgc cgccgctctc acaggacgcg gatcccgttg gtccccaggg tgttgatgga
     2401 ggggtcaaag aatttgccgc ccccgcggaa atagggctct tcgatcaggg ccgcgtgata
     2461 gcccgccacc agagaaccgg gcacaaaggc gtaaagcggc agcagaaaac ggaactgccg
     2521 gtccggcacg ggcaggatgc actccatcac cggggcggtg acgcccagct cggcggccgg
     2581 cttgtcggcg accaccaaca ccttgcggcc cagcttttcc acctggccgc tgatggtctc
     2641 ggccatacgg gcgcagttgc cctcgtcgct gaccgcatag tcgatggtgc cgtaggcgtt
     2701 ttggggcatc atgatgctgt tgacatgcca gaagttctcc gagtccacca ccgtgcactt
     2761 gtcgccggag acctccgcgt acttggcggg gatgaactcg ccgcagcaga agagcgggcc
     2821 gtcggcgcag acctcaaagc ccttgctgtc tttcatggcg acggcggcgg cgtaggcgct
     2881 gtcgtccatc tgctccagcg cgtcggcaaa acggccgttg taggcctcca gcgcggcccg
     2941 cagcccgtcg cagaggccgg tcttgccgct tttcacctcg cccagcacgg cggccagcac
     3001 gaacaggccc atcatggagg cgagatagct gcgcaggccg ggcatccggt cggggaactc
     3061 cggcgtgttg gtcacatagg ccaggtcggc ggtggtgccg gcgcgggagg caccgttgtt
     3121 ggtcagcgcc atggtcaggc agccgtgctg tttgccgcgc tgcaggatct cggcgatgcg
     3181 cgccgggccg cccgaggcgc tgatggcgat cagcagggtg ccggcggcgt cttcctccag
     3241 cggcagatag cgggcggcct cgatggcccg caccgcctcc atgcggcagc cggtgccctt
     3301 gagatagtgc ttgaagacct cccgcgcctg tgccgcggcc agataggagt cgccgcagcc
     3361 ggtgaccacc acgcggcgga tgccggccag gcgtttttca ggctccaggc gcagcagggc
     3421 ctcgcgggcg aggtcgaact gggccggcgc cagacgcacc aggtcaaagc actggcggcg
     3481 cagcgggtta tcgtagtttt gtttcatgtt gcattccccc ctcatcacat tttccaggta
     3541 cgggttctga aatcatagcg gttgctgaag aacagctttc cggccagggc ggcgcagtag
     3601 gccgccagca gagagccggg cgcatggtcc atcatcagca gcgcccaggg ggcgcactcc
     3661 ggcgctttgg ggagccggca gaccgcggcg ccctcggcga aatcgcagtc cgcgtcggtg
     3721 accaccagcg tgggtcgccc gatggcgacc gagacccgca cgctctcggc gatgcggtcg
     3781 aggtcggggg cggcggcatt cacctggaag atcgtaccca ccgtttccgg ctcgcgcagg
     3841 aaaaagttga tgtggcacca gtcctcggaa tccgcgtggg aggcgtggac gctggcgcac
     3901 tcgatgaatt tctcttccac gaactgggcg gagaaaaatt cactctcatc gcccacgatc
     3961 tcaaactttt caaggccgct ccagcgctgg gccagctcga acatctggtc gtcgatctcc
     4021 tggtagcggg gctggcaggc ggacacatag tccaggatgg cctgccgcgc gactgcgggc
     4081 tcggccacac ccttgctgcc gccgatgtag gaggccagcg ccgccagcgc cgtgaggctg
     4141 gcaaaatagc tgcgcagccc cggggtgttg cacagctccg gcgtctcgac gcagtacacc
     4201 agctctgcgg ccttggcgcc cttggactcc gggttgttgg tgatcagcat cgagcgcacg
     4261 cctgcggccc tggcccgctc cagcatctcc acgatccgca cgctgccgcc gctggcgctg
     4321 atggcgacga ccagcacctc ctccggcttg cagccgtccc ggatatcctc gtcggtgtaa
     4381 aagctgcaca gctccatcgg gtcgggcgag gcgcagctca cgcaggccag tttgcggatc
     4441 acgggggcca tggcccccgc cgccgagtaa gagtcgccgc agcccgcgac gatcagccgc
     4501 ttgagggcgg ccgcctcggg caaggggaga agcgccgaga gctttttttc atcgaaacac
     4561 ttttccacct ggatgggggc gagctcgaac aagctctcca cctgcaggcc gatggtgttg
     4621 tagtaattct gtttatccaa tcgttggtac cccctgtctt tgatgcgccg cggcgcggtc
     4681 ttgcgatcgc gccgcgacgg aaattacgat tcgccggacc ccggggcgga ctttttggcc
     4741 tgagcctctt cccagagctg cttttctctt tcctcctcca ggcgctggcg gttcatcttc
     4801 agcagacggt ccgcctgctt ggagcgccag aacgacatgg cgaagtagag caggacgatc
     4861 atgataaagg gcagagcggt caggaaatac tggtccacga catagggctt caggtagttg
     4921 gtgacggcgt acgccaggcc gaagacgaag ctcatcagca tcgagccgat gggcatgccg
     4981 ttggcgatgg tcgcggccgc caggccaacg aagccgcggt tggcggtgat gttgcggatg
     5041 aagaagttct ggtagcccat cgacaggtac atgccgccga agctgcccac gaaagccgcc
     5101 agcgcaaagg ccagcgtgta gatcttgcgg gggttgatgc ccaccgactc ggccgcctgc
     5161 gggttttggc ccacagcgcg catgcgcagg ccgattttgg tgcggaacag caaaaaccag
     5221 accgcgaaca ccatgaagat ggagaaatag gtgaacacgt tgtggccgga caggatcgag
     5281 ccgatgaacg ggatatcctt gataaagggg atgtccacgt tgcccagcgc aaggctcttg
     5341 acgacgccgg cagtggtggc cttggtgccg gtcaccaggt agaccacgaa caccgtgccg
     5401 cccagcaggg ccatgttcag cgagatggac atcaggtaga ggtcgacttt catgataaag
     5461 gtggcgaagc acaggaacag cgcgatcagc actgcggtca gcgcgccgcc caaaatgccc
     5521 agccagacgt tctgggtcca ggcgctgaac agcacgccgc ccagcgacgc ggtcagcatc
     5581 atggcctcgg cggccatgtt cagcaggccg gcctgctgcg agatggtgga gcagatcgac
     5641 acaaagatca gcggcgttgc gatgcggatc agagaataga taaagttgct aacaaattca
     5701 agcattgctc tttgcctcct ttaccttgac cgcttcctcc tcgcgggaga tgttgaagat
     5761 gacgcggcgc ttggtcttgc ccaggaactc ctcggccgcg acgaacagca cgatgaccgc
     5821 ctgcagcatg gtgaccaact caatggggat attggtgttg gcgttgagca ccgaggctgc
     5881 ggtgcggata taggccagaa cgaaagcggt gagggggacg aacagaggct gtttgcgggc
     5941 catgaccgcg cacagcaggc cgtccatgcc cacgttggtg aggatctgat agtagtagct
     6001 gttataggtg ccgaagcagt ccacggccgc cgccacgccg gcgaacaggc cgccgatcag
     6061 ctgggccagg tacatcatct ttttcgcatt gatgcccgag taggtcgcaa aggagaggtt
     6121 ggagccgcac atgcggatct tggcgcccag cggggtgcgg tagaagagga tacaggccac
     6181 gatgacgccg atgatcgcca cccagatcgc ggaggtgaag ttggtgtcgc cccaatagcg
     6241 ggtgaaccgc atgttgtccg ggaagatcgg ggaggtcagg aaagactggc cgcgatcggc
     6301 cacggtggac cgcaacagcc acagggccag atacaggcac atgtagttca tgatggtgga
     6361 ggtcaccatc tcgttggcgc ccagcttttc acggccgaac gcgggcacca tggcaaaggc
     6421 gccgcctgcg atggcgcaga ccacgatgat gatgatcagg ttgacgaact ggggcaggtt
     6481 ggtcatcagc ggggtccgga acatcaccat cagggcgaag atcggggcga tgttgatgat
     6541 gccctcgctg atgaggctga agcggccgca ggcgtagata aagcacatgc cgcagccggt
     6601 gagcaggtag ggcgcatagc gggccagcac ctggccaaag cgccgcgggg acatgaacgg
     6661 gcccacgacg aagttgtaga ccgcgtcccc ggcgctctcc tcgctgatga gcacgatgat
     6721 gacaatgcag gccaaaaagg cgatcacgat acccaaaaag acgcggagaa attcgaacat
     6781 gtattcaact ttttgtacct ttttcaaaac aaatccccca tttcctcggg cgtcatgctc
     6841 ttgacaccga gcatgtattc gccaagctgc tccgcagtga ccgatttcac atcgttgaac
     6901 tgggcgacga tctggccgtc tttcatgacc agcagccggt ccgagacctc caggatctcg
     6961 ttcaggtcgg aggagatcag cagcgcgccg atcttctttt cccgggtgta ctttaccagc
     7021 agcctgcgga tcaggtcggt ggtgcccacg tcgatgccgc gggtgggctg gttggcgacg
     7081 atcaggttcg cgccgctgga gaattcgcgg gcgatgatga ccttttggat attgccgccg
     7141 gacagcagat ggatggcctc gtcgccgccg gtgcaggcga tttcaaactc ctcgatgcag
     7201 tggtcc
//
LOCUS       DBMDYA010000041         7206 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2681_length_7206_cov_1.518649, whole genome shotgun sequence.
ACCESSION   DBMDYA010000041 DBMDYA010000000
VERSION     DBMDYA010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_7206_cov_1.518649"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="ACZPFK_01815"
     CDS             complement(<1..563)
                     /locus_tag="ACZPFK_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1405077.1"
                     /translation="MIYSINGPVVTVRDTKSFEMAEMVYVGDKRLVGEVIGVTDRATT
                     IQVYEVTTGLRVGEPVTGSGSPLSALLGPGIIGNIFDGIERPLGDIAAKTGAFIPAGV
                     AADSLDFDKPWAVTMAVRVGDQVKGGDVFATCPETSIITHKSMVPPDVAGEVVFAAGD
                     GPHTLRDVLCRVKLADGSERELTLAQR"
     gene            complement(613..1191)
                     /locus_tag="ACZPFK_01820"
     CDS             complement(613..1191)
                     /locus_tag="ACZPFK_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR1405078.1"
                     /translation="MTELDSKGSFRKFEDAVIDGAERKAAAIVVEAEQYRQSELRAAS
                     QGSGAAELERKRQELLTARSQYAAKRRQESRRQLLEARRAMAQSLFDEVEGRIAAFTA
                     TAGYDAWLTGRAQRHAALAGQGVTVLLRPADESKQGLLRAVFPQASFETDRAIRCGGF
                     KLVVGRVLYDETLDAAFAAEQERFTAESGLRV"
     gene            complement(1266..1574)
                     /locus_tag="ACZPFK_01825"
     CDS             complement(1266..1574)
                     /locus_tag="ACZPFK_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR1405079.1"
                     /translation="MKYFLISDNTDTLAGMALVGVPGVVVHEPNEVEAALQKACDDPE
                     VGIVLLTDKLSQNCRETVFRYKLGCSRPLLVTMPDRHSGDDAAADIKRYISEAVGIRI
                     "
     gene            complement(1589..1927)
                     /locus_tag="ACZPFK_01830"
     CDS             complement(1589..1927)
                     /locus_tag="ACZPFK_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR1405080.1"
                     /translation="MNRMKKAVRILLSCMLVFLLAAALSTVFGAEAGESAEAAAATAR
                     GVGMIAAALSTGIAAIGSGIAVGAGAPAAIGALTEDPKTFGKSMIFVALGEGIALYGM
                     LISILILNKV"
     gene            complement(2012..3946)
                     /locus_tag="ACZPFK_01835"
     CDS             complement(2012..3946)
                     /locus_tag="ACZPFK_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR1405081.1"
                     /translation="MAIEKMKYINIYGPQSELVRVLGVLTELACFHPDTAGKEEFRPN
                     VSDNPYAALLGEAQGLLADLHAGEEETPALEQFELADVRAMIGGYQKKLADSTEKYKG
                     LLEEKTLYAHTRHQLRHLEKMDVNLDDVFSLQYVKVRFGRLPKEGYRKLPYYADREFS
                     FNSFDFDGEYYWGFYFTPEQIADEVDAIFASLHFERLRVPDFVHGTPGEALIQIAEHE
                     AQLNAKIEELTTPTQEKTRQDLGTIRRMCVWLDREAQLYDMEKYAQVMGGTFYLSGFV
                     PKAESKRVIERLTAECDCKVSDPIASADAPVPPPIRLKNNWFARPFEMYVSMYGLPSY
                     KDVDPTGFVAVTYSILFGIMFGDVGQGLCVCVAGFLMAKFLKLPLGSILTRCGVFSMF
                     FGLCYGSVFGYEHLLDPLYQAVGLSGKPLEVLAPENTNFILIGSIAIGVVVILASILF
                     GLVSKAKRGAKGALIFSANGVAGLVFYGAIIAGAASLLLLDRNLFTPVFIVAGLVIPF
                     LLMYFQEPLAQWVDHHKVKLESGKAEYVASAFFEMFDTLLTYASNTMSFLRVGGFVLA
                     HAGMMSVVMTLAEMVGAGAAPVVVVIGNLFVMGMEGLIVGIQALRLEFYEVFSRFFDA
                     DGVPFTPLTLRAAHAARARS"
     gene            complement(3989..5029)
                     /locus_tag="ACZPFK_01840"
     CDS             complement(3989..5029)
                     /locus_tag="ACZPFK_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR1405082.1"
                     /translation="MLQKKSSLALIPKARGLFAHRLTEGEYDELMHKRSVAEIGAYLK
                     NHPYFRDSLSGIQDTQVHRRRLEELLSRDIYYKYEALRRYDFSSGSFAHFFIVDCEVQ
                     ELLYALHLLATGSPGDYITRLPSFLADQTCFDLLALARARSFADILRAVAHTPYHRLL
                     QPLLTSPAGFNYAACEHALRSYYYETALALVDKTFNRREAAEVRELFTREAEIYNLDI
                     IYRIKAFFPDSYSAAEIEALLLPVSCEIPPRRLQEMARARGLKELATAYKGYGYDRAY
                     GDFEQENSSACAWRHLYQVSARILRFSSSPGTVLAAVLLLAKIERGNLITIIEGVRYG
                     MDPANIRVLLKY"
     gene            complement(5112..5423)
                     /locus_tag="ACZPFK_01845"
     CDS             complement(5112..5423)
                     /locus_tag="ACZPFK_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405083.1"
                     /translation="MEKLIESIIAADKKARVIVEKAESKREAILRETREQKAQAAESY
                     RSSFARAGARLDESEQAEIARIAAAEEADLAGRRQALCDEFEAHRAAWADEMLRNITA
                     V"
     gene            complement(6018..6977)
                     /locus_tag="ACZPFK_01850"
     CDS             complement(6018..6977)
                     /locus_tag="ACZPFK_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1405084.1"
                     /translation="MKLVLLDSYAMREGDLDWSQLRALVDGFTAWPRTPYEEAAARIG
                     DADFVVVNKTWIDDAVLDACPNVKWIGLTATGTDSLDLDACRRHGVPVSNVPGYSTHS
                     VAQHVFSLLLSICQCPDRYYRAVKGGCWQTDIRPEYGVTPQRELFGKTLGIVGYGDIG
                     RSVARIAQGFGMNVIAHTRTVRDAYLGDGVKFMPLDELLAAADVVTLHCPATPKTRGM
                     ICDETLAAMKPGAILINTARGALVDDAAVARALHSGRLGFFGADVVGAEPIRPDNPLL
                     GCDNALITPHIAWATRESLDRLAAIVTDNFRSYLAGQPKNLVT"
BASE COUNT         1288 a         2286 c         2414 g         1218 t
ORIGIN      
        1 cagcgctgcg ccagggtgag ctcgcgctcg ctgccgtcgg ccagcttcac gcggcagagc
       61 acgtcgcgca gggtgtgggg gccgtccccg gcggcaaaga ccacctcgcc cgccacatcg
      121 gggggcacca tgctcttgtg ggtgatgata ctggtctcag ggcaggtggc gaagacgtcc
      181 ccgcccttga cctggtcgcc cacccgcacg gccatggtga cggcccaggg cttgtcgaag
      241 tccaggctgt cggcagcgac gcccgcgggg ataaaggcgc ccgtcttggc ggcgatgtcg
      301 cccagcgggc gctcgatgcc gtcaaaaatg ttgccgatga tgccggggcc cagcagggcc
      361 gagagcgggc tgccggagcc ggtgaccggc tcgccgaccc gcaggccggt ggtgacctcg
      421 tagacctgga tggtggtggc cctgtcggtc accccgatga cctcgcccac caggcgcttg
      481 tcgcccacat agaccatctc ggccatctca aagctcttgg tatcgcggac cgtgaccacc
      541 ggcccgttga tagaataaat cacgtctgtc aaatggtgcc cctccttttc ggtgttccgg
      601 tatgagggcg ggtcataccc gcaggccgct ctcggcggtg aagcgctcct gctcggcggc
      661 aaaggccgcg tccagcgtct cgtcgtagag cacccggccg acgaccagct tgaagccgcc
      721 gcagcggatg gcccggtcgg tctcaaagga cgcctgcgga aaaactgcgc gcagcagccc
      781 ctgtttgctc tcgtcggccg ggcgcagcag tacggtgacc ccctgaccgg ccagggcggc
      841 gtgccgctgc gcccggccgg tcagccaggc gtcgtagccc gccgtggcgg taaaagccgc
      901 gatgcgcccc tccacctcgt caaaaaggct ttgggccatg gcgcgccggg cctccagcag
      961 ctggcggcgg ctctcctgcc gccgcttggc cgcgtattgg ctgcgggcgg tgagcagctc
     1021 ctgccgcttg cgctccagct cggcggcgcc gctgccctgg ctggccgcgc gcagctcgct
     1081 ctggcggtac tgctccgcct cgaccacgat ggccgcggcc ttgcgctccg cgccgtcgat
     1141 gaccgcgtct tcaaatttgc gaaaactgcc tttggaatcc agttcagtca tatcaactta
     1201 ccatgccttt ccggccccct gtcggccagg gccgattcct ggcgccgccg cgcactgcgc
     1261 cggcgtcaga tgcggatgcc caccgcctcg gagatatagc gcttgatgtc cgccgccgcg
     1321 tcgtcgccgc tgtggcggtc gggcatcgtg accagcagcg ggcggctgca gcccagcttg
     1381 tagcggaaaa cggtctcccg gcagttctgg gacagcttgt cggtgagcag cacgatgccg
     1441 acctcggggt cgtcgcaggc cttttgcagc gccgcttcca cctcgttcgg ctcgtgcacc
     1501 accacgccgg gcaccccgac cagcgccatg ccggcgaggg tgtcggtgtt atcgctgatg
     1561 agaaagtatt tcacctaggc tcacaccctt agaccttatt gaggatcagg atcgagatca
     1621 gcatgccgta cagcgcgatg ccctcgccca gagccacgaa gatcatgctc ttgccgaagg
     1681 tcttggggtc ctcggtcagg gcgccgatgg cggcgggcgc gcccgcgccg acggcgatgc
     1741 cggagccgat ggccgcgatg ccggtggaga gggcggccgc gatcatgccc acaccgcggg
     1801 cggtggccgc agcggcctcg gcgctctcgc ccgcctcggc gccgaaaacg gtggagaggg
     1861 cggcggccag cagaaagacg agcatgcagg acagcaggat gcggacggct tttttcatac
     1921 ggttcataat ggaatcctcc caaatcttaa aatcaaatcg gatgtctcga tgcggtcaag
     1981 cgcgtcgccg cgcagaggga cggagcaggg ctcagctgcg cgcgcgggct gcatgggctg
     2041 cgcgcagggt gagcggggta aaggggacgc cgtcggcgtc gaagaagcgg ctgaacacct
     2101 cgtagaactc aaggcgcagc gcctggatgc ccacgatcag gccctccatc cccatgacga
     2161 agaggttgcc gatgacgacc accaccgggg ccgcgccggc gcccaccatc tctgccaggg
     2221 tcatgaccac gctcatcatg cccgcgtggg ccagcacaaa gccgcccacc cgcaaaaagc
     2281 tcatggtgtt gctggcgtag gtcagcaggg tgtcgaacat ctcaaagaaa gcgctggcca
     2341 catattcggc cttgccgctc tccagcttga ccttgtgatg gtccacccac tgggccagcg
     2401 gctcttgaaa atacatcagc aaaaacggga tcaccagccc ggcgacgata aagaccgggg
     2461 tgaaaaggtt gcggtcgagc agcagcagcg aggccgcgcc ggcgatgatg gccccgtaaa
     2521 agaccagacc cgccacgccg ttggcggaaa agatcagcgc acccttggcc ccgcgcttag
     2581 ccttggagac cagcccgaac aggatcgagg ccaggatgac caccacgccg atggcgatgg
     2641 agccgatgag gatgaagttg gtgttttccg gcgccagcac ctccagcggc ttgccggaga
     2701 ggccgacggc ctggtacagc gggtcgagca ggtgctcgta gccgaaaacg ctgccgtagc
     2761 acagcccgaa gaacatcgag aagacgccgc agcgggtgag gatcgagccc agcggcagct
     2821 tcaggaattt ggccatcaaa aagcccgcga cgcacacgca caggccctgc cccacgtcgc
     2881 cgaacataat gccgaacagg atggaatagg tgacggccac aaagccggtg gggtccacat
     2941 ccttgtagct gggcaggccg tacatcgaga catacatctc gaacggccgc gcgaaccagt
     3001 tgtttttgag ccggatgggc ggcggcaccg gcgcgtcggc gctggcgatg gggtccgaga
     3061 ccttgcagtc gcactcggcc gtcagccgct cgatcacccg cttgctctcg gccttgggca
     3121 caaagccgga caggtaaaag gtgccgccca tcacctgggc gtatttttcc atgtcgtaga
     3181 gctgtgcctc ccggtccagc cagacgcaca tgcggcggat ggtgcccagg tcctggcggg
     3241 tcttctcctg ggtgggggtg gtcagctcct cgatttttgc gttcagctgc gcctcgtgct
     3301 cggcgatctg gatcagcgcc tcacccggcg tgccgtggac gaagtcgggc acccgcagcc
     3361 gctcaaaatg caggctggcg aagatggcgt ccacctcgtc ggcaatctgc tcgggggtga
     3421 agtaaaagcc ccagtagtac tcgccgtcaa agtcaaagga gttgaagctg aactcgcggt
     3481 cggcgtagta gggcagcttg cggtagccct ctttgggcag gcggccgaag cgcaccttga
     3541 catactgcag tgaaaagacg tcgtccaggt tgacgtccat cttttccaga tggcgcaact
     3601 ggtgccgggt gtgggcatag agggtttttt cctccagcag tcccttatat ttttccgtgc
     3661 tgtcggccag ctttttttgg tagccgccga tcatggcgcg cacgtcggcc agctcaaact
     3721 gctccagggc gggcgtctcc tcctccccgg cgtgcagatc ggccagcagg ccctgggcct
     3781 cgcccagcag ggcggcgtag gggttatccg aaacgttggg gcggaactcc tccttgccgg
     3841 cggtgtcggg gtggaagcag gccagctcgg tgagcacccc cagcacccgc accagctcgc
     3901 tctgcgggcc gtagatattg atgtacttca tcttttcaat agccaaaaac atgacctcct
     3961 caggggcgga acagacatct ttacgccctt agtattttaa gaggacgcgg atgtttgccg
     4021 ggtccatgcc gtagcggacg ccctcgatga tggtgatgag attgccgcgc tcgatcttgg
     4081 cgagcagcag cacggcggcc agcaccgtgc ccggcgagga agagaagcgc aggatgcgcg
     4141 ccgagacctg gtagagatgc cgccaggcgc aggccgagga gttctcttgc tcgaaatcgc
     4201 cgtaggcgcg atcatagccg tagcccttgt aggcggttgc cagctcttta aggccgcggg
     4261 cgcgggccat ctcctgcagg cggcggggcg ggatctcgca gctcaccggc agcagcagcg
     4321 cctcgatctc ggcggcggaa tagctgtccg ggaaaaaggc cttgatgcgg tagatgatgt
     4381 ccagattgta gatctcggcc tcgcgggtga acagctcccg cacctcggcc gcctcgcgcc
     4441 ggttgaacgt cttgtccacc agagccaggg cggtctcgta atagtagctg cgcagcgcgt
     4501 gctcgcaggc cgcgtaattg aacccggcag gggaggtcag cagcggctgc agcagccggt
     4561 ggtagggcgt gtgggccacc gcccgcagga tgtcggcaaa gctgcgggcg cgggccaggg
     4621 ccaacaggtc gaagcaggtc tggtcggcca gaaagctggg cagccgggtg atatagtccc
     4681 ccgggctgcc ggtcgccagc agatggagag cgtagagcag ctcctgcacc tcgcagtcca
     4741 cgatgaaaaa atgcgcaaag ctgcccgagg aaaaatcata gcggcgcagc gcctcatatt
     4801 tataatagat gtcgcgggag agcagctcct ccaggcgtcg gcggtggacc tgggtgtcct
     4861 ggatgccgga gaggctgtcg cgaaaatagg gatggttttt gagataagcg ccgatctcgg
     4921 cgacgctgcg cttgtgcatc agctcgtcgt attccccctc ggtcaggcgg tgggcaaaga
     4981 gcccgcgcgc cttggggatc agcgccagtg aggacttttt ttgcagcatg ggaccacctc
     5041 cttacaaaaa aatgacggcc cggccgccgg agggcaaaag agacgagccc ggcccaggtc
     5101 gtctctcggc gtcaaacggc ggtgatattg cgcagcatct cgtccgccca ggcggcgcgg
     5161 tgggcctcga actcgtcgca gagggcctgg cggcggccgg cgaggtccgc ctcctccgcg
     5221 gcggcgatgc gggcgatctc ggcctgctcg ctctcatcca gccgcgcgcc ggccctggca
     5281 aagctgctgc ggtagctctc ggcggcctgg gctttctgtt cccgggtctc gcgcaggatg
     5341 gcctcgcgct tggattcggc tttttcaacg atcacccggg cctttttgtc ggcggcgatg
     5401 atactttcaa tcagtttttc cacgcggaca cctcccggca gccgcgcgcc ctcaaaagcg
     5461 gcggttgcca actattgata ataaataaaa attgacttaa tgttggaaag cgatactaat
     5521 tatagtctct atggaatcga ttttcaaggg aacgagttgc caaataaatg ccagatacct
     5581 accgcgaaac tgggaaaaac aaataaaaat ctcgattccg tgactgtgcc gtccggagag
     5641 atgaaaaaat cgtttccaca aatgccgcgt aaggcagaag cgcacgaggc ttttgcgcgc
     5701 tttttcggcg gcagcggccg gtgggctgct ggctgccaga gcgtctgcag ccagcgcttg
     5761 ccccgggaaa tagggcgtcg ggccgccagc ggcactgccc tgcgcaggaa aagcggcggg
     5821 gcagtccagc tgcctgcacc ctgcggcccc gggccggcgg ggcgccctgc aagggcccct
     5881 ctctcagggg gccttgcagc gcgcggcttt ttgcccctgc ccggcggggc ggacagaaaa
     5941 atgggaaaaa agaagaccag cgggcggacg cgcagagcgg cgtccccgct gatcttcaac
     6001 gacagtggag cgtgcggtca ggtgaccagg tttttcggct gcccggccag atagctgcgg
     6061 aaattgtcgg tcacgatggc ggccagccga tccagggatt cccgcgtggc ccaggcgatg
     6121 tgcggggtga tcagggcgtt gtcgcagccc agcagcgggt tgtcgggccg gataggctcg
     6181 gcccccacca catcggcccc gaaaaagccg agcctgccgc tgtgcagggc gcgggccacg
     6241 gccgcgtcgt ccaccaacgc gccgcgggcg gtgttgatga ggatggcgcc gggcttcatc
     6301 gccgccagcg tctcgtcgca gatcatcccc cgggtcttgg gggtggcggg gcagtgcagg
     6361 gtgacgacgt cggccgcggc cagcagttcg tccagcggca taaactttac cccatcgccg
     6421 agataggcgt cccgcacggt gcgggtgtgg gcgatcacgt tcatgccaaa gccctgggcg
     6481 atgcgcgcca cagagcggcc gatgtccccg tagccgacga tgcccagcgt cttgccgaac
     6541 agctcccgct gcggggtgac gccgtattcc gggcggatat cggtctgcca gcagcccccc
     6601 tttacggcgc ggtagtagcg gtcgggacac tggcagatgg agagcagcag ggaaaagacg
     6661 tgctgggcca ccgaatgggt ggaatagccg ggcacgttgg acaccggcac tccgtggcgg
     6721 cggcaggcgt ccaggtccag gctgtcggtg ccggtggcgg tgaggccgat ccacttcacg
     6781 ttggggcagg cgtccagcac tgcgtcgtcg atccaggtct tgttgaccac cacaaaatcg
     6841 gcgtcgccga tgcgggcggc tgcctcctca tagggggtgc ggggccaggc cgtgaagccg
     6901 tccaccaagg cgcgcagctg cgaccagtcg aggtcgccct cccgcatggc gtaagagtcg
     6961 agaaggacca gtttcatgag agattcctcc tttgggcggg cgctgacgcg ccgccgtttc
     7021 ggcggccctc agcgggcgag gagaagctcc ccgcccggcg agagggcgac cccgcgccag
     7081 ccctcctcca ggtagcggcc cagccgcttg gtctccgaga gagggcagcg cagggctgcc
     7141 ggatcctcct tattataagt agtttccccc tgcggcgcaa gcgcaaagat aaaacaggcg
     7201 caaagg
//
LOCUS       DBMDYA010000042         7190 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2690_length_7190_cov_1.662294, whole genome shotgun sequence.
ACCESSION   DBMDYA010000042 DBMDYA010000000
VERSION     DBMDYA010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_7190_cov_1.662294"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            144..617
                     /locus_tag="ACZPFK_01855"
     CDS             144..617
                     /locus_tag="ACZPFK_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1405085.1"
                     /translation="MKQLQDPVLREVGALFRSVHALYDAKYRSLDLQRGQFIFLTRIC
                     ERPGIRQTDLAALLRVDKTTVAKVVPKFLRNGYLTRKRDGHDGRVWRLYPTPRALELY
                     DLVIEEENRSARVCFAGFSAGERAAARSLLRRMRQNCEADLEAYRRRQKNDQKSD"
     gene            598..1092
                     /locus_tag="ACZPFK_01860"
     CDS             598..1092
                     /locus_tag="ACZPFK_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1405086.1"
                     /translation="MIRKATESDLPAVLAILRDTRAEMASYGNDQWNDDYPQAADFLA
                     DIRAGSLWLLEEADDPLAFVCLNGDEPEEYAAVGWRFDGAPLVLHRMAVSPAARRKGV
                     GSRLMAFAEEQARAQGLRCVRSDTYSINEKMNRLFLRCGYRQAGTMHFKSRPLLFYCY
                     EKQL"
     gene            1247..2437
                     /locus_tag="ACZPFK_01865"
     CDS             1247..2437
                     /locus_tag="ACZPFK_01865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1405087.1"
                     /translation="MEKSRLEALSQRYLEETLEARRYIHRHPELSWKEFGTQAYVLEA
                     LRRHGIECRGDFEGTTVIGMIRGGHPGETVALRADMDALPVDEASGCAYPSENGGVMH
                     ACGHDMHTSSLLGAAFMLQELRQELHGDVKLIFQPAEEAGATQCGAEVLVQKGILENP
                     HVDAIFSAHVFPTVPLGKVGVCEREMMAGVDIFKLKIHGTGGHLSTPHRARSALYPAL
                     QFMNEISVLRTQAVDPFDTAIIDTGYFHCGTAENIIPAEAEIVGNVRAYNDDLRVELK
                     RRMENIARGLELAFDVKCDYEHRFGYDSLINDPAMAKLYRGACEEVFGAENIVTPQPA
                     MGSEDFAFYLKKVKGAFVWLGVGDDGSGSEGLHSDVFFPSEKALPLAMQMLTNVAVRY
                     LNRE"
     gene            2868..3254
                     /locus_tag="ACZPFK_01870"
     CDS             2868..3254
                     /locus_tag="ACZPFK_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1405088.1"
                     /translation="MKNAIKKLPDSELEAMLAVWRAEPPVRGSEIAAALAESRGWNKS
                     TVFTLLLRLVEKGFLFCEKEGKNNLYRPLVSEEDYRARESRSMLDRFYAGSLKNFVAA
                     FCSEEPLSRSDLEELRRLVDEKAKEL"
     gene            3508..4488
                     /locus_tag="ACZPFK_01875"
     CDS             3508..4488
                     /locus_tag="ACZPFK_01875"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR1405089.1"
                     /translation="MSIRFASGAEDEALADRFAAAHPLGSFMQSPRWALVKPKWGHTV
                     LLSQREDGVLRGSMLVLTLADRGDGKALLYAPRGPVCAPHDLHALADLFAGARMLASR
                     FGRGEFKCDPLIGEGDAEAVAELTALGLSFAPGAKFRETVQPRQNAVRRGLAGMSEGQ
                     LLASFSAKTRYYVRRAAAQGVVCAPCGRQALPEFYALYEETGRRQGFAVRPAAYLEAI
                     LQAFGQDARLYLCRSPEGEPLAGSVAVVFGPRLSFVYGASSRSRPELAACYLLQWEML
                     RFALERGCDLYDMGGICTDEAESPALFQLYTFKKKFAPAESLAGEFGFVF"
     gene            4635..5495
                     /locus_tag="ACZPFK_01880"
     CDS             4635..5495
                     /locus_tag="ACZPFK_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1405090.1"
                     /translation="MNQKVILSADSPCDLGPELQQRYDVHFYPFHILLGDASYTDSVD
                     IQPDDLYRAWHERKLLPKTAAITPLEYAEYFRQWVEAGYEVVHVNIGSALSSGYQNCC
                     AAARALGHVYPVNSCNLSTGIGLLVLRAGELIRQGMPAAEIQQVLNALVPCSHASFVL
                     DTLEFMRAGGRCSAVTAFGANLLHLKPCIQVHTEKEGAMGVGKKYRGAMETVLPVYIR
                     DQLSAYADPVPDHVFLTHSGVPESWLQVSRKAVEAAGIFKEIHVTRASCTISSHCGPG
                     TLGVLFMTER"
     gene            complement(5928..>7190)
                     /locus_tag="ACZPFK_01885"
     CDS             complement(5928..>7190)
                     /locus_tag="ACZPFK_01885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405091.1"
                     /translation="QQNAMGRVNGYIEEMIDGQKVVKVFCHDSKAKQEFDALNDELCH
                     AATNANTFANILMPIMGNLSYIHYAVTAIAGAVMAIGGGMDIGTIASFLQYTRSFSQP
                     ITQISQQFNNVLIALAGTERIFEVMDEKPEVDDGYVTLVNMERGEDGQLREADHHTGL
                     WAWKHPHKTGGLDYTPVTGDVVFDHVTFGYDEKKMVLRDISLYAKPGQKIAFVGSTGA
                     GKTTITNLINRFYDVPDGKIRYDGINVNKIKKDDLRRSLSMVLQDTHLFTGTVRDNIR
                     YGKLDATDAEIERAAKIANADGFIRHLPQGYDTLLTGDGANLSQGQRQLIAIARAAVA
                     DPPVLILDEATSSIDTRTEALIEKGMDGLMEGRTVFVIAHRLSTVRNSNAIMVLENGE
                     IIERGTHDELLAQKGKYYQLYTGMFELS"
BASE COUNT         1250 a         2265 c         2257 g         1418 t
ORIGIN      
        1 ttttaccgcc tgtctgcaag gcgcctcttt ttacacggat cagcagttga catcgccgca
       61 aaaaaccttt atgcttttag ttgaaaattc aactttttaa cctgtctttt tattcttcgg
      121 gcaggtggga aagggaggga gccatgaagc aactgcaaga ccccgttctg cgggaggtgg
      181 gcgcgctgtt ccgcagcgtc catgccctgt atgacgccaa ataccgcagt ctcgacctgc
      241 agcgtgggca gtttatcttt ttgacccgca tctgtgagcg ccccggcatc cgccaaaccg
      301 acctggcggc cctgctgcgg gtggataaga ccacagtggc gaaggtggtg ccgaaatttt
      361 tgcgcaacgg atatctcacc cgcaagcggg acgggcacga cgggcgggtg tggcggcttt
      421 accccacgcc ccgcgcgctg gagctctacg acctggtgat cgaggaggag aaccgcagcg
      481 cccgcgtctg ctttgccggc ttcagcgccg gggagcgggc cgccgcccgc agtctgctgc
      541 gccgcatgcg gcaaaattgc gaggccgatc tggaagctta tcgaaggagg caaaaaaatg
      601 atcagaaaag cgactgaaag cgatctgccc gccgttttgg cgattctgcg ggacacccgc
      661 gccgagatgg caagctacgg caacgaccag tggaacgacg attatccgca ggcggcggat
      721 tttttggcgg atatccgggc gggcagcctc tggctgctgg aggaagcgga cgaccctctg
      781 gcttttgtct gcctcaacgg cgacgagccg gaggaatatg cggcggtggg ctggcgcttt
      841 gacggcgcgc cgctggtgct gcaccgcatg gccgtgagcc cggcggcgcg ccgcaagggc
      901 gtgggcagcc gcctgatggc ctttgccgag gagcaggccc gggcgcaggg cctgcgctgc
      961 gtccgctccg acacctattc catcaacgaa aagatgaacc gccttttcct ccgctgcggc
     1021 tatcgccagg ccggcaccat gcactttaaa agtcgtccgc tgctttttta ctgctatgaa
     1081 aagcagctgt gagcggcgcc ttgccctttg accggcgctc agccggggcg ttttttcttt
     1141 ccgccgcccc ggggccgcat tttgctcttg tatttctgcg cgatctcctg taagataaga
     1201 aaaggttatt ttaatgcttc aacggagtaa agagaggagt gtcgagatgg aaaagtcgag
     1261 actggaggcg ctgtcacagc ggtacctgga ggaaacgctg gaggcgcggc gctatatcca
     1321 ccgccacccc gagctgagct ggaaagaatt cggcacccag gcctatgttc tggaggcgct
     1381 gcggcgccac ggcatcgagt gccgcggcga ctttgagggc accaccgtca tcggcatgat
     1441 ccgcggcggc caccccggcg agacggtggc cctgcgcgcc gatatggacg cgctgccggt
     1501 ggacgaggcc agcggctgcg cctatccgtc ggaaaacggc ggggtcatgc acgcctgcgg
     1561 gcatgacatg cacacctcca gcctgctggg ggcggccttt atgctgcagg agctgcggca
     1621 ggagctgcac ggcgacgtca agcttatttt ccagcccgcc gaggaggccg gcgccaccca
     1681 gtgtggcgcc gaggtgctgg tgcaaaaggg cattctggaa aacccgcatg tggacgcgat
     1741 cttttccgcc cacgtcttcc ccaccgtgcc gctgggcaag gtgggcgtct gcgagcggga
     1801 gatgatggcg ggcgtggata tcttcaagct gaagatccac ggcaccggcg gccatctctc
     1861 caccccgcac cgggcccgca gcgccctgta cccggccctg cagttcatga acgagatctc
     1921 ggtgctgcgc acccaggcgg tggacccgtt cgacaccgcc atcatcgaca cgggctactt
     1981 ccactgcggc acggcggaaa atatcatccc tgccgaggcc gagatcgtgg gcaatgtgcg
     2041 cgcctacaac gacgatttgc gggtggagct caagcgccgg atggagaaca tcgcccgcgg
     2101 cctggagctg gcctttgacg tcaagtgcga ctacgagcac cgcttcggct acgacagcct
     2161 catcaacgac cccgccatgg caaagcttta ccggggggcc tgcgaggagg tcttcggcgc
     2221 tgagaatatc gtgacccccc agcccgccat gggctctgag gacttcgcgt tctacctcaa
     2281 aaaggtgaaa ggcgcttttg tctggctggg cgtgggggac gacggctccg gcagcgaggg
     2341 gctgcacagc gacgtcttct tccccagtga aaaggccctg ccgctggcga tgcagatgct
     2401 gaccaacgtg gccgtgcgct atctcaaccg ggagtgatcc cctccccgcc ctttggcccc
     2461 ccgcctgccg gggggctttt cacttttgtc cgcgcagcga cgcgtgggca agccgccctt
     2521 gctttcgcgc ccgctttctg cctttttgcc gcggcgatcc ccggccctgc tctccccgcc
     2581 gcgccgggtc gtcccttgca actttttaca aaattctcca aaaaaccttg actttccctc
     2641 cagtgggcgg taatcttatt ttataaagct acaattgtaa actaaatcgg ctgaggaggg
     2701 cttgtcatgg gcgctcgaaa aaccgcacct gcgtttttgg accatctgcg agagattttt
     2761 gcctgggacg cctgcgccgc tgcggatgag cagcaaccgc gcgatgcctt tttgccggat
     2821 agccgtgccg ccggtcacag cggcgaggaa aaaggggaat ccgtaaaatg aaaaacgcca
     2881 tcaaaaaatt gccggactca gagctggagg ccatgctcgc cgtctggcgc gccgaacccc
     2941 cggtgcgggg cagcgagatc gccgcggccc ttgctgagag ccgcggctgg aacaagagca
     3001 ccgtctttac cctgctgctg cggctggtgg aaaagggatt tttgttctgt gaaaaagagg
     3061 gcaaaaacaa cctctaccgc cctctggtga gcgaagagga ctatcgcgcc cgcgagagcc
     3121 gctcgatgct ggaccggttt tacgccggct cgctgaaaaa tttcgtcgcc gccttttgca
     3181 gcgaggagcc gttgagccgc agcgacctgg aagagctgcg ccgcctggtg gatgaaaaag
     3241 ccaaagagct ctgaggtccc atctccggca cggcgaccgc cgccgctctt ctccggtttt
     3301 gccccattcg caaaggcgcc gcttccgctc tcgcttttca ccccccaccc gcatacgcgc
     3361 acggcctctg ccgtacattt tgggcagggg ccgtcttttt acggcacaaa attacatttg
     3421 taagttttcc gccgattctc ttgcgcccca cctttgaacc ccacaaagac gacggcccgc
     3481 gatcattttt tcaaggagga aagaagtatg agtatccgct ttgcatccgg cgctgaggat
     3541 gaggctcttg ccgaccgctt tgccgccgcc catccgctgg gcagctttat gcagtcgccc
     3601 cgctgggcgc tggtaaagcc gaaatggggc cacacagtgc tgctctccca gcgggaagac
     3661 ggtgttttgc gcggcagcat gctggttctg acgctggcgg accggggcga cggaaaggcg
     3721 ctgctctatg cgccgcgcgg gccggtctgc gcccctcacg acctgcacgc gctggccgac
     3781 ctgtttgccg gagcccggat gctggcgtcc cgctttgggc ggggagaatt caaatgtgac
     3841 ccgctgatcg gcgaggggga cgcggaggcg gtggccgagt tgaccgccct gggcctctcg
     3901 tttgcaccgg gggcaaagtt ccgcgagacg gtgcagccgc gccagaacgc ggtgcgccgc
     3961 ggccttgcgg ggatgagcga ggggcagctt ttggcctctt tttccgccaa gacccgctat
     4021 tatgtgcggc gggccgccgc gcagggtgtg gtctgcgccc cctgcgggcg gcaggccctg
     4081 ccggaatttt acgctcttta tgaggagacc gggcggcgtc agggctttgc cgtgcgcccg
     4141 gcggcctatc ttgaggcgat cttgcaggca tttgggcagg acgcacggct ctatctctgc
     4201 cgcagcccgg agggcgagcc gctggccggg tcggtggcgg tggtgttcgg cccccggctc
     4261 agctttgtct acggcgcctc cagccgcagc cgccccgagc tggccgcctg ctatcttctg
     4321 cagtgggaga tgctgcgctt tgcactggaa cggggctgcg acctgtacga tatgggcggc
     4381 atctgcaccg atgaggcgga gtcccccgcc ctgttccagc tctacacttt taaaaagaag
     4441 ttcgcccctg cggagagcct ggcgggggaa ttcggctttg tcttctgacc gccccgcttt
     4501 cgctcccctt tcatccggtt tgatggaagc ctcttctatt tttacagaaa gcttgtccca
     4561 ggccccgtaa agtggtattc ttttccatag cggatgcagc aagttcagca aacgccatga
     4621 aaaggagttt ttatatgaat caaaaggtca ttttaagcgc ggacagcccc tgcgatctgg
     4681 gcccggagct gcagcagcgc tatgacgtcc acttttaccc gttccacatc ctgctgggcg
     4741 acgcctcgta taccgacagc gtggacatcc agcccgacga tctctaccgc gcctggcacg
     4801 agcgcaagct gctgcccaag acggcggcca tcacgccgct ggagtacgcc gaatacttcc
     4861 gccaatgggt ggaggcgggc tatgaggtgg tgcacgtcaa tatcggctcc gccctctcct
     4921 ccggctatca gaactgctgt gccgcggccc gcgcgctggg ccacgtctac ccggtcaact
     4981 cctgcaacct ttccaccggc atcggcctgc tggtgctgcg cgcgggggag ctgatccggc
     5041 aggggatgcc ggcggcggag atccagcagg tcctcaacgc cctggtgccc tgcagccacg
     5101 ccagctttgt gctcgacacg ctggagttca tgcgcgcggg gggccgctgc agcgccgtga
     5161 ccgcctttgg ggccaacctg ctgcacctca agccctgcat ccaggtccac accgaaaagg
     5221 agggcgctat gggggtgggc aaaaaatacc gcggcgccat ggaaacggta ttgcccgtct
     5281 atatccgcga ccagctctcc gcctatgcgg acccggtgcc ggatcatgtc tttctcaccc
     5341 actccggcgt gccggagagc tggcttcagg tctcccgcaa ggcggtggag gcggccggga
     5401 tcttcaaaga gattcatgtc acccgggcca gctgtaccat ctcctcccac tgcggccccg
     5461 gcacgctcgg ggtactgttc atgaccgaga ggtaggcctc tcttggagct ttttacccgg
     5521 acgcggcacg ccggccgcct ctctcaaaag cagcgcgggc ttttcgttcc accctgctac
     5581 ggagcggccc gcccccttga ggcgccgcat gggagacgcg ggcgcggcgc ccgttccccc
     5641 tctgcccttt tccgtctcta tcctatcctg aaacagggaa gaataaaaag cgcactgcaa
     5701 tgcagtgcgc ttttttcgta aaaccagcgt cccagccgcc ccaaagaggg ggacagtggg
     5761 catgccgggc ctgatgttca aaatccaaag ccggccggca cggggcagcc ggagaaaacc
     5821 gcggggccgc cagccggcct ggttcccctc tcgcgcggtg gcggggaaca ggggcgtccc
     5881 gtccgtacct tggagaccgc cccccggcat ccggccaccg ccgcgcgcta agacaactcg
     5941 aacataccgg tgtagagctg gtagtacttg cccttttggg ccaacagctc gtcgtgggtg
     6001 ccgcgctcga tgatctcgcc gttttccagc accatgatgg cgttggaatt gcgcaccgtc
     6061 gacagccggt gggcgatgac gaagaccgtg cgcccctcca tcaggccgtc catccccttt
     6121 tcgatcagcg cctcggtgcg ggtgtcgatg ctgctggtcg cctcgtccag gatcagcacc
     6181 ggcggatcgg ccaccgccgc ccgggcgatg gcgatcagct gccgctgccc ctgtgagaga
     6241 ttggccccgt cgccggtgag cagcgtgtcg tagccctggg gcagatggcg gataaagccg
     6301 tcggcgttgg cgatctttgc cgcgcgctcg atctccgcgt cggtggcgtc cagcttgccg
     6361 tagcggatat tgtcccgcac ggtgccggtg aacaggtggg tgtcctgtag caccatggac
     6421 agcgagcggc gcagatcgtc ctttttgatc ttgttcacat tgatgccgtc atagcggatc
     6481 ttgccgtccg gcacgtcgta aaagcggttg atgaggttgg tgatggtggt cttgcccgca
     6541 ccggtggagc cgacaaaggc gattttctgc cccggctttg cgtagagcga gatatcccgc
     6601 agcaccatct ttttttcgtc atagccgaac gtgacgtggt cgaacaccac atcccccgtc
     6661 accggcgtat agtccaggcc gccggtcttg tgggggtgct tccaggccca gaggccggtg
     6721 tggtggtccg cctcccgcag ctgtccgtcc tccccgcgct ccatattgac cagcgtcaca
     6781 taaccgtcgt ccacctctgg cttctcgtcc atgacctcaa agatgcgctc agtgcccgcc
     6841 agggcgatga gcacgttgtt gaactgctgc gagatctggg tgatgggctg ggagaaagag
     6901 cgggtgtact gcagaaaaga ggcgatggtg ccgatatcca tgccgccgcc gatggccatc
     6961 accgcgccgg cgatggcggt gaccgcatag tggatgtaag agagattgcc catgatgggc
     7021 atcaggatat tggcaaaggt gttggcgttg gtggccgcgt ggcagagctc gtcgttgagg
     7081 gcgtcgaact cctgcttcgc cttgctatca tggcaaaaaa ccttgaccac cttttgcccg
     7141 tcgatcatct cctcgatata gccgttgacg cggcccatgg cgttttgctg
//
LOCUS       DBMDYA010000043         7113 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2724_length_7113_cov_1.696663, whole genome shotgun sequence.
ACCESSION   DBMDYA010000043 DBMDYA010000000
VERSION     DBMDYA010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_7113_cov_1.696663"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="ACZPFK_01890"
     CDS             complement(<1..1172)
                     /locus_tag="ACZPFK_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405092.1"
                     /translation="MEHKNDSVDEILKQLKDGRTSAETAPFSGDEVDDILNSLGLEPP
                     KKAAGRPQRQGPAQVMGPSAEELAPKSARPAPSAAAEQRQTAAQRPAPAPVKRAAAPS
                     REGAVEYIQPDKPTAAPPAPLDDGGEFVRTSPDIFSGEKIDRLSHADELIDEEFYKFF
                     TRSVVVDKAEVDRRLKEEKRRKKKEKKARRGLFSFLRAEPEDEDGEDELYEAQEHPRE
                     EQPAPGDIFAAAPEQELPPIRRRQAPAPAAPEADPALFSWAAAPEGKAERDEKYSTSE
                     IVIEEERATRPARAAGPSAVPPPAPPRAPTTEEEPVQRETPEPQRSAAQAAPTGAPAA
                     GDAPAAEEAFFPEDLPESGRGGRATRVFDTVPETPLTPRAEAAEDAEDADEREAED"
     gene            complement(1207..1971)
                     /gene="rlmB"
                     /locus_tag="ACZPFK_01895"
     CDS             complement(1207..1971)
                     /gene="rlmB"
                     /locus_tag="ACZPFK_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1405093.1"
                     /translation="MEQAQNTVVYGKNAVTELLKSGGGVDTVYLADGMAPAAASYYTA
                     LAKEAGAAVKRVHTAKLRALCGTENHQGVAAFASAVEYASLDDLLALAAQRGEDPFLL
                     LADGVEDPHNLGAMIRTALLAGAHGIVIPKRGGAQVTPIVGKTSAGASVVLPIARVAN
                     IGEAVRRLKEKNVFVYGADMAGRAMGSQDLTGPIALVLGSEGSGISPLVKRLCDGLVS
                     LPMSPHRAGVDSLNVSVAAGVLMYEVVRQRGGFGEK"
     gene            complement(2130..2387)
                     /locus_tag="ACZPFK_01900"
     CDS             complement(2130..2387)
                     /locus_tag="ACZPFK_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405094.1"
                     /translation="MKGVNKKVIEINEPESEYIEKVLVFLRQKDGHVNVARARQEAEG
                     YVNTLVCWRRLPLNLPPKKLLLAGGLCLLAIAAVVAGILLL"
     gene            2888..3784
                     /locus_tag="ACZPFK_01905"
     CDS             2888..3784
                     /locus_tag="ACZPFK_01905"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1405095.1"
                     /translation="MEWSYEDGTLTVRPDGPFSAAETLFCGQCFRWRQGPGCFEGVVH
                     GRALTLRERGDGAVLLTGLAESDAPYFLHYFAFDEDYAALQRRLERSKRLAECIRCAP
                     GIRVLHQDFFEVLLTFIISQNNNIPRITGIVARLCEGFGAPLPGGGHAFPLPGDLAGR
                     TAEELAFLRAGWRAGYLLDAAQKVASGEVGQERLARLSTPEARALLMTIRGVGPKVAD
                     CVLLYGLGRAEACPMDVWMKRAMARRFPRGMPRCARELAGVAQQYIFHAERTAPQPQA
                     GDSEKKRAPKPGPAGEKNRPAP"
     gene            4187..4948
                     /locus_tag="ACZPFK_01910"
     CDS             4187..4948
                     /locus_tag="ACZPFK_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012859.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HIR1405096.1"
                     /translation="MAKTEFVSNRYYTIAAKTTGRVLEVADFNLENGGLIQLWDDAGA
                     ESQQWKIIEAGDGFYKIVCKHSEKAVDITVGGTQDGAWVHQWDFVGADNQLWSIEAAG
                     DGCYKIKSKLGGKCLDVVGMNAVNGANIQIWEDVDGDNQKWVITALPAKAEKKDAAPA
                     EKPAAKKAPAKKPAAKKTAAKKPASAKKPAVKAEAAKAEASKAEKSPAVKKSPVKKAA
                     AKPETVKAAESAVKASPAKAEAPKAEKKPAAPKKK"
     gene            complement(5333..6097)
                     /locus_tag="ACZPFK_01915"
     CDS             complement(5333..6097)
                     /locus_tag="ACZPFK_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1405097.1"
                     /translation="MSWFFFEGRRVWYEESGAGKPLLFLHGNAASSNMFFELAERYRR
                     QFRVVLLDFLGNGRSQRLSRWPADLWFDEAQQAIALLRERGYEKVDLIGCSGGALAAV
                     NVALEAPALVGRVIADSFEGERPLPAFTESVREQRARSKREEAARQFYFYMHGADWEQ
                     VVDRDTDAILRHSRQIGAFFHRPLCGLEAELLLTGSREDEFTAALSPDYLQRAYGALL
                     QKVGHGRMHLFDHGAHPAMLSNPEAFYQLSMEFLQG"
     gene            complement(6094..>7113)
                     /locus_tag="ACZPFK_01920"
     CDS             complement(6094..>7113)
                     /locus_tag="ACZPFK_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405098.1"
                     /translation="DRALLDAAVDHVLSINRADLELQQGNYSSWKENRDRRDAWELAR
                     DERLRGEIRRMEEAAARSADWGRAVEASKYGARNSGLRVDRGYIGAKAARMMQSAKNL
                     QNRQARALEEKRSLLQNLEEEAPLKISALAPARRQLLRAEGLTVGYGARPLFAPLDFV
                     LEQGQRLALTGPNGSGKTTLLRLLAGQQVRHGGLLWRQNGLSVSVVPQDTGSLRGPLR
                     YWPRDNGLDPPLFFALLRKLGFERSQFELDAERCSEGQKKKLLLAASLARPAHLYLWD
                     EPLNYLDVASREQIEELLLAGRASMIFVEHDVRFVERVATGKITLERREAAPHCAGRG
                     EGARG"
BASE COUNT         1136 a         2456 c         2260 g         1261 t
ORIGIN      
        1 tcgtcttccg cttccctctc gtccgcgtcc tccgcgtcct ctgcggcctc cgcgcgcggc
       61 gtcagagggg tctcgggcac cgtgtcgaag acgcgggtgg cgcggccgcc tctcccgctc
      121 tccggcaggt cctcggggaa aaaggcctct tcagcggccg gcgcgtcccc cgccgcgggc
      181 gcgcccgtgg gggccgcctg cgcggcagag cgctggggct cgggcgtttc cctctgcacg
      241 ggctcctctt ccgtcgtggg cgccctgggg ggcgcgggcg ggggcacggc agacggtccg
      301 gcggcccgag ccggacgggt ggcccgctcc tcctctatca cgatctctga ggtgctgtat
      361 ttttcgtctc tctcggcctt tccctccggg gccgcagccc aggaaaaaag agccgggtcc
      421 gcctcggggg ccgccggggc gggcgcctgc ctgcggcgga tggggggcag ctcctgctcg
      481 ggggccgcgg cgaagatgtc gccgggcgcc ggctgctcct cccgcggatg ctcctgcgcc
      541 tcgtagagct cgtcctcgcc gtcctcgtcc tccggctccg cccgcaaaaa agagaacagc
      601 ccgcgccggg cttttttctc ctttttcttt ctgcgcttct cctctttgag acggcggtcc
      661 acctccgcct tgtccaccac cacggatctt gtgaaaaatt tatagaattc ctcgtcgatc
      721 agctcgtccg catggctgag gcggtcgatc ttttcgccgc tgaaaatatc cggagaggtg
      781 cgcacgaatt cgccgccgtc gtccagcggc gccggcgggg cggcggtcgg cttgtcgggc
      841 tggatgtatt ccaccgcgcc ctcccgcgac ggggccgcgg ccctttttac cggcgcgggc
      901 gcagggcgct gcgccgccgt ctgccgctgc tccgccgctg cggacggggc ggggcgggcg
      961 ctcttcggcg ccagctcctc cgctgagggg cccatcacct gggccgggcc ctgccgctgc
     1021 gggcggccag ccgctttttt cggcggctcc agccccaggc tgttcaggat gtcgtccacc
     1081 tcgtccccgg aaaagggcgc cgtctcggcc gaggtgcggc cgtctttcag ctgtttgagg
     1141 atctcgtcca cgctgtcgtt tttatgttcc aactgggtcc tgcctccctg tctgctgcgc
     1201 tatgtcttat ttttcgccga agccgccgcg ctggcgcacg acctcataca tcaaaacccc
     1261 cgccgccacc gagacgttca ggctgtccac cccggcccgg tgggggctca tgggcaggct
     1321 gaccagcccg tcgcacaggc gcttcaccag cggcgagatg ccgctgccct cgctgcccag
     1381 caccagggcg atggggccgg tgaggtcctg gctgcccatc gcccgcccgg ccatgtccgc
     1441 cccgtagacg aagacgtttt tttctttcag ccgccgcacc gcctcgccga tgttggccac
     1501 ccgcgcgatg ggcagcacca cgctggcccc ggccgaggtc ttgcccacga tgggggtgac
     1561 ctgcgcgccg ccccgcttgg ggatgacgat gccgtgcgcc ccggccagca gcgcggtgcg
     1621 gatcatggcg cccaggttgt gggggtcctc caccccgtcc gccagcagca ggaacgggtc
     1681 ctccccccgc tgcgccgcca gggccagcag atcgtccaac gaggcgtatt ccaccgcgct
     1741 ggcaaaggcc gccaccccct ggtggttctc ggtgccgcaa agcgcgcgca gctttgccgt
     1801 atgtacgcgc ttgactgccg cgcccgcctc tttcgccagg gcggtataat aggacgcggc
     1861 ggccggcgcc atgccgtcgg cgaggtagac cgtgtccacc ccgccgccgc tcttgagcag
     1921 ctctgtcact gcgtttttgc cgtacacgac cgtgttctgt gcctgttcca tctgcgcctc
     1981 ccgctgcggg ccgagaaagg ggcttttccg ccccgcgccg cccgcatgca tatatattgt
     2041 acattatagc aactttcaca cggcatttcc accgcctgga acagatgtcc gaaaattctc
     2101 ttaccgggcg gctgtggcgg cccgcgcgct cagagcagca ggatgcccgc caccaccgcg
     2161 gcgatggcca gcaggcacag gccgccggcg agcagcagct ttttcggcgg caggttcagg
     2221 ggcagccgcc gccagcacac cagcgtgttg acatagccct ccgcctcctg ccgggcgcgg
     2281 gccacgttga cgtggccgtc tttctggcgc aaaaagacca gcaccttttc gatgtattcg
     2341 ctctcgggct cgttgatctc gatcaccttt ttattgacgc ctttcatacc ttgtctcccc
     2401 tttcgtcgct ttccatccca gtatggcctg gccagctctc tcttaaacaa gggcaaggcc
     2461 gcgccccgcg ggcgcgggga gagggcaaaa gcccccttgt atggcagagg aaaaacttgt
     2521 caagactcaa aatcttgcgc ccttgcgggg ggattataca aagagtttat gggcctttcc
     2581 ctcctctttt ctcccggtgc agtgttgaaa accctgttga aactgtggac aaacggcgga
     2641 taaggccgca aaaccgggtg ttgagaggct ggtttttaac atagttttcc acacgctgtt
     2701 gaaagctctc cacaaaaccg gttgttaaaa aatttttgtc aagatggcaa ttgtgctgtt
     2761 tttcgactgg taaatttatc aaatttttcg attttcacaa aagcccccgc tttccctatc
     2821 ttgctcttta tgctatactg gacgatacca aaatttcgac tttttgcaac aaaaaggggg
     2881 gcgaccggtg gagtggagct atgaggatgg gacgctgacg gtccggccgg acgggccttt
     2941 ttccgccgcc gagaccctgt tttgcggcca gtgcttccgc tggaggcagg ggcccggctg
     3001 ctttgagggg gtggtgcacg gccgcgccct caccctgcgc gagcgcgggg acggcgcggt
     3061 gctgctcacc ggtcttgctg agagcgacgc gccctatttt ttgcactact tcgcctttga
     3121 cgaggactac gccgcgctgc agcggcgtct tgagcgctca aagcgcctgg cggagtgcat
     3181 ccgctgcgcc cccggcatcc gggtgctgca ccaggacttt ttcgaagtgc tgctcacctt
     3241 tatcatcagc cagaacaaca atatcccccg catcaccggc atcgtggcgc ggctgtgcga
     3301 gggcttcggc gcgccgctgc cgggcggcgg gcacgcgttt ccgctgcccg gggacctggc
     3361 cggccgcacg gcggaggagc tggcctttct gcgcgcgggc tggcgtgcgg gctatctgct
     3421 ggacgccgcg caaaaggtgg cctcggggga ggtggggcag gagcgtctgg cccgtctttc
     3481 caccccggag gcccgcgccc tgctgatgac catccgcggg gtgggcccca aggtggcgga
     3541 ctgcgtgctg ctgtacggcc tggggcgggc ggaggcctgc ccgatggacg tgtggatgaa
     3601 gcgcgccatg gcgcgccgtt ttccgcgcgg gatgccccgc tgcgcccggg agctggcggg
     3661 cgtggcgcag cagtacatct ttcacgcgga gcgcaccgcc ccccagcccc aggccgggga
     3721 cagcgaaaaa aaaagagcgc ccaagcccgg gcccgcgggg gaaaagaacc gccccgcccc
     3781 gtgagccgga aagcggggcg acaagaacgg gggcgttttc ccgcgggacc gcgccgtgaa
     3841 gcgtggattc agatgcgccg cgcgctgaag ccatcgcctt gccgtgaggt tttgccgggc
     3901 cccgctccag gtctgaggag gccgggccgg tcacccgccg cgtgggggcg ggacttttgc
     3961 ccctgatgca ggcggctcga acggcccatt ttccgccgcc gcctccgcgg cccgtttgta
     4021 acctccttgt aaccttgtgt ttttttgaca tttttcacct tgcccatctg tacaaatacg
     4081 gaaagcggga tttttcaggc ttatgcaaag cgccaaaagt ggctgcaagg cgcttgactg
     4141 cctgtcccgc tttggtatat agtcaacccc aaaggagtgt tgcgatatgg caaaaaccga
     4201 atttgtttct aatagatact acaccatcgc cgccaagacc accggccggg tgctggaggt
     4261 cgcggatttc aacctggaga acgggggcct catccagctg tgggacgacg ccggcgccga
     4321 gagccagcag tggaagatca tcgaggccgg ggacggcttt tataagatcg tctgcaagca
     4381 ctcggaaaag gccgtcgaca tcacggtggg cggcacacag gacggcgcct gggtccatca
     4441 gtgggacttt gtgggcgcgg acaaccagct gtggtccatt gaggcggccg gcgacggctg
     4501 ctacaagatc aaatccaagc tgggcggcaa gtgcctggac gtggtgggca tgaacgcggt
     4561 caacggcgcg aacatccaga tctgggagga tgtggacggc gacaaccaga aatgggtcat
     4621 caccgccctg cccgccaaag cggaaaagaa agacgccgcc ccggcggaga agcccgccgc
     4681 gaaaaaagcc cctgcgaaaa agcccgccgc caagaaaacc gcggccaaaa agcccgcgtc
     4741 cgcgaaaaag cccgccgtca aggcagaggc cgcaaaagcg gaggcgtcca aggcggaaaa
     4801 aagcccggcc gtcaagaaga gcccggtgaa gaaagccgcc gccaaaccgg agaccgtcaa
     4861 ggccgcagag tccgccgtga aagcgtcgcc cgcaaaggcc gaggccccga aagcggagaa
     4921 aaagcccgcc gcccccaaga agaaataacc cgtcacaaga caagagagcc cggcagccac
     4981 gctgccgggc tctttttttc tgcgttttca ccggggccgg cccccgcgct gtgcggctca
     5041 ggcggaccct gcaccgcgcg cccggtggag cgccggggcc tcggccgccc tgcccgcaaa
     5101 acccgccgcg cagaagctcc ccctgcggaa aagcggttct ccgccgcttc cggtgcaagc
     5161 gccgccctct tcccctcaac agctcccctt tcgctcaaaa gagcgcggcg tgcgggaagc
     5221 gctgccgaga gacgggcatc cgcggacggg gttcccccca actgccgctt tctctcaaaa
     5281 ggcacttcca cgcccatcaa aacgcttctt tcggtaattt ttgacccttt ctctatccct
     5341 gtaaaaactc catgctcagc tgataaaacg cctcggggtt tgacagcatg gcggggtggg
     5401 cgccgtggtc gaacaggtgc atgcggccgt gcccgacctt ttgcagcagc gcgccgtagg
     5461 cccgctgcag gtagtccggc gagagggcgg cggtgaactc gtcctccctg ctgccggtca
     5521 gcagcagctc ggcctcaagg ccgcagaggg gccggtggaa aaaggcgccg atctgccggc
     5581 tgtggcgcag gatggcgtcg gtgtccctgt ccaccacctg ctcccaatcc gcgccgtgca
     5641 tataaaaata gaattgccgc gcggcctcct cccgcttgga gcgcgcccgc tgctcgcgga
     5701 cgctctcggt gaacgcgggc agcgggcgct ccccctcaaa gctgtcggcg atcacccgcc
     5761 ccaccagcgc cggggcctcc agcgccacgt tgacggcggc cagcgccccg ccgctgcagc
     5821 cgatgaggtc caccttttca tagccccttt cgcgcagcag cgcgatcgcc tgttgcgcct
     5881 cgtcgaacca gaggtccgcc ggccagcggg acaagcgctg tgagcgccca ttgcccaaaa
     5941 aatccagcag caccacccgg aattgcctgc ggtatcgttc cgcgagctca aaaaacatgt
     6001 tggaggaggc ggcgttgccg tgcaggaaca gcagcggctt gcccgcgccg gattcctcat
     6061 accagacccg cctgccctcg aaaaagaacc agctcatccg cgcgccccct ctccccggcc
     6121 cgcgcagtgg ggcgcagcct cccggcgttc cagcgtgatt ttaccggtgg ccacccgctc
     6181 cacaaagcgg acgtcgtgct ccacaaagat catggaggcc cggccggcga gcagcagctc
     6241 ttcaatctgc tcacgcgagg ccacgtccag atagttcagc ggctcgtccc agaggtagag
     6301 gtgggcgggc cgggccaggc tcgccgccag cagcagtttt ttcttctgcc cctccgagca
     6361 gcgctcggcg tccagctcga actggctgcg ctcaaagccc agcttgcgca ggagggcgaa
     6421 aaagaggggc gggtccagcc cgttgtcccg gggccaatac cgcagcgggc cgcgcaggct
     6481 cccggtgtcc tgcggcacca ccgagacact gagcccgttc tgccgccaca aaaggccgcc
     6541 gtggcgcacc tgttgcccgg ccagcaggcg cagcagcgtt gttttgccgc tgccgttggg
     6601 gccggtgagg gccagccgct ggccctgctc caaaacaaag tcgagcggcg caaagagcgg
     6661 ccgcgcccca tagcccaccg tgagcccctc ggcgcgcagc agctgccgcc gcgccggggc
     6721 cagggcgctg atcttcagcg gcgcttcctc ctccaggttt tgcagcagcg accgcttttc
     6781 ctccagggcg cgggcctggc ggttttgcag gttttttgcg ctctgcatca tccgggccgc
     6841 ctttgccccg atatagcccc ggtcaacccg caggccgctg ttgcgggcgc cgtatttgct
     6901 ggcctccacg gcgcgccccc aatccgcgct gcgggcggcc gcctcctcca tgcgccggat
     6961 ctcgccccgc agccgctcgt cccgcgccag ctcccaggcg tcgcggcggt cgcggttttc
     7021 tttccagctg gaatagttgc cctgctgcag ctccaggtcc gcccggttga tagacaggac
     7081 atggtccacc gccgcatcca gcagggcgcg gtc
//
LOCUS       DBMDYA010000044         7093 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2733_length_7093_cov_1.655177, whole genome shotgun sequence.
ACCESSION   DBMDYA010000044 DBMDYA010000000
VERSION     DBMDYA010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_7093_cov_1.655177"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="ACZPFK_01925"
     CDS             complement(<1..190)
                     /locus_tag="ACZPFK_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405099.1"
                     /translation="MKIRCRSCGKRFSYEEQDGICPHCGAYNSVSWDVEPGGGDLSPS
                     EPTVCDAPEWHAEAGHYDE"
     gene            complement(234..569)
                     /locus_tag="ACZPFK_01930"
     CDS             complement(234..569)
                     /locus_tag="ACZPFK_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405100.1"
                     /translation="MTVREINLKYDMPPADVAVRRMEIELSAGRHTGQKLFKFIHGYG
                     SSGTGGKIRVAVRRALCGRQGVAWVLPGESFGPFSAEARDAAQRWPALRQDRDWGREN
                     DGVTVVVLR"
     gene            805..2247
                     /locus_tag="ACZPFK_01935"
     CDS             805..2247
                     /locus_tag="ACZPFK_01935"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR1405101.1"
                     /translation="MERNETLMQYFEWYLPADCGHWNRTAAAAPALAAQGITGVWLPP
                     AYKGAAGREDVGYAVYDTYDLGEFDQKGSVPTKYGTRDEYLNAVRTLQGAGVKVYADV
                     VLNHRMGADGCEEVLAEEDDGRDRNREVGGEERIRAYTRFDFPGRGGKYSDFRWNWRH
                     FDGVDWDDADKKKGIYQFEGKQWDRDVDGENGNYDYLMGADLDMDDPEVLAELDRWGA
                     WYLQTTGVDGFRLDAVKHIRFTFFKDWLGKLRRQSGKPLFAVGEYWKQDTGALLHYLD
                     ECGGCMSLFDVPLHFNFYAASHGGGGYDMRNILKGTLLEACPDSAVTFVDNHDTQPGQ
                     ALQSWVDGWFKPLAYALILLRQQGLPCVFYGDYYGIPHDNINPVGGPLLALLRVRAER
                     AFGPQHDYFDDPNVIGWTREGDAEHPGSGLAAVLSDGAGGPKRMYVGQRHAGATFAPV
                     TGGGTAVLISPDGWGDFSVDGGNARVYVRI"
     gene            2370..3890
                     /locus_tag="ACZPFK_01940"
     CDS             2370..3890
                     /locus_tag="ACZPFK_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR1405102.1"
                     /translation="MKARPRLYWAFNICFGVFFIGLCIVSIFWHDNPFVQMKKSLLLG
                     TTVTFLVVALGAVILWQRIAWPLHHEIRTIVVLMTLYLLLQLLFGWQMQVGFDAEWDY
                     PIVAKAAEELVLHGVPPGEYFALFSNNGPLFWFYTGYFHLLYWFGSQDFVYGLIILNS
                     LMIDLSFFFLYLTARQLWGKRGALPLLGAAMLCPAFLLYVPIAYTDTLTLPFVTGAVW
                     LWLCARSRRMEGLPSGRWVAGALALTALGAVLKMSVAVLAFAFALDLLVWWDGRGRLR
                     LLAIGAACSATLWTGVNFASRAAMPPFEEKSVPFTHWIMMGLHGNGGYWDPDYELTLQ
                     YDTYEERLVFTQGEILRRLKEMGTEGLAKHCVAKLSYIISDGTCYAPEKLDRNHRGGE
                     ALNDFILKGGKYSSFLYYGADGLQLCLLALCAVGSFLSLPRQQQQVTVFRIGVFGLLL
                     FLLLWEARSRYLVNFLPLLLLCAADGLASLYKRQRHSPDGISSSRKEMRKWDLNIR"
     gene            complement(4049..5113)
                     /locus_tag="ACZPFK_01945"
     CDS             complement(4049..5113)
                     /locus_tag="ACZPFK_01945"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1405103.1"
                     /translation="MLEIKIERTAHPKPKPEGPLGFGSIFTDHMFLMDYTADRGWHDP
                     RVVPYAPIVLDPAAQVFHYAQECFEGMKAYRTADGRVQLFRPDKNGERLRSTHERLCI
                     PPIPVEDFVQAVKALVRADRDWVPSEEGTSLYIRPFTIATEAQLGVKASGAYQFCIIA
                     SPSGSYYPTGIDPVRIYVEDEYVRAAPGGTGAVKCGGNYAASLLSGEKAHRLGYGQVL
                     WLDGVERRYVEEVGSMNCFFKMDGVIHTAPTTGTVLPGITRMSCIELLEGWGYRVKVD
                     RLPIDEVMRADREGTLEEVFGTGTAAVISPVGELKYKDQVAVINGGRIGPVTQRLYDT
                     LTGIQYGRLPAPQGWVTEVE"
     gene            complement(5318..6256)
                     /locus_tag="ACZPFK_01950"
     CDS             complement(5318..6256)
                     /locus_tag="ACZPFK_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR1405104.1"
                     /translation="MKHPHLITRLAAGSICLALICALAACGGSTAADHFSAAPAAAEQ
                     ATGGETENYDASLTDVKAQSDRKLIVNAELWLDATDFEAAAAEIERQVTALGGYLASS
                     NQDGAAGTANRSARYEARIPAEKLSSFLDSAGKAGAVVSSSQSTTDVTAEYVDNEARL
                     ASLRTQEQRLLELMAQAAKLEDLITLEDKLTEVRYQIESLTGQQKLYDNQVEYATVSV
                     IVNEVTRETITAPTFGDRILRAFQGSLDNVVELAQELVILFIYLLPLLLLIAVVLTAV
                     LLSLRAARRRRAKRPAATQPPADIPPAAGDRGPGRQ"
     gene            6628..>7093
                     /locus_tag="ACZPFK_01955"
     CDS             6628..>7093
                     /locus_tag="ACZPFK_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1405105.1"
                     /translation="MDKQAMLNWLEGQEGMLSKMARAIWEKPELAMEEVFASGLQRDA
                     LAAAGFQVREVPGVPTAFAAEYGHGKPVLGILGEYDALPDLSQQQGPERRALVEHGPG
                     HGCGHNLLGTAGLGAAMALAKAIESGALAGTVRYYGCPAEETISGKTLMAKAH"
BASE COUNT         1350 a         2004 c         2321 g         1418 t
ORIGIN      
        1 gttcatcata gtgtccggcc tcggcatgcc attcaggagc atcgcagacc gtgggctcgg
       61 acggagagag gtcgccgccc cccggctcca catcccaact gaccgagttg tatgcgccgc
      121 agtgcgggca gatgccgtcc tgctcttcat aagaaaaccg cttgccgcag cttctgcatc
      181 ggatcttcat ctcagaccac cctctatgtt ctgcccggag catccggggc gtttcagcgc
      241 agcaccacca cggtgacgcc gtcgttctcg cggccccaat ctcgatcctg ccgcagtgcg
      301 ggccagcgct gcgccgcgtc ccgcgcctcg gcggaaaagg ggccgaagct ctcacccggc
      361 agcacccagg ccacaccctg cctgccgcac agcgcacggc gcacggcgac gcggatcttg
      421 ccgcccgtgc cggaggagcc gtaaccgtgg atgaatttga acagcttctg ccctgtgtgc
      481 cggccggccg agagctcgat ctccatccgc cgcaccgcca catcggcggg cggcatgtca
      541 tatttcaggt tgatctcccg caccgtcatc cggccgcgcc ccctttttgc actctggttg
      601 aaagtatatc aaaacagggc ccggggcgca aggccgcggg acgcgcccag ccctcacccg
      661 cccggcggca ggtcaggggg cagccccaaa tttgcgcccg ccctctcaga aacggaaaaa
      721 gaaaagattt tttcacaagg aaaggcacat ttgggtgtaa aataggaggc ggacgcaaaa
      781 aacagggaaa agtgaggcag actgatggag cgcaacgaga cgctgatgca atattttgag
      841 tggtatctgc ccgcggattg cgggcactgg aaccggacgg ctgcggctgc gcctgccctt
      901 gcggcgcagg ggatcaccgg ggtctggctg ccgcccgcct acaagggcgc cgcgggccgg
      961 gaagacgtgg gctacgccgt ctatgacacc tatgatctgg gcgagttcga ccaaaagggc
     1021 tccgtgccca caaaatacgg cacgcgggat gaatacttga acgccgtccg gaccctgcag
     1081 ggcgccggag taaaggtgta tgccgacgtg gtgctcaacc accgcatggg ggccgacggc
     1141 tgtgaagagg tgctggcgga ggaggacgac gggcgggacc gcaaccggga ggtgggcggg
     1201 gaggagcgca tccgcgccta cacccgcttt gactttccag gccggggcgg aaaatacagt
     1261 gatttccgct ggaattggcg ccattttgac ggtgtggact gggacgacgc ggacaagaaa
     1321 aagggcatct accagtttga gggcaagcag tgggaccggg acgtggatgg ggagaacggt
     1381 aactacgact acctgatggg ggccgacctg gatatggacg acccggaggt gctggccgag
     1441 ttggaccgct ggggcgcctg gtacctgcag accaccgggg tggacggctt ccggctggat
     1501 gccgtcaaac acatccgttt cacctttttc aaagactggc tgggcaagct gcgccggcag
     1561 agcggcaagc cgctgttcgc cgtgggggag tactggaaac aggacaccgg cgcgctgctg
     1621 cactatctgg acgagtgcgg cggctgcatg agcctgttcg atgtgccgct gcacttcaac
     1681 ttctatgcgg cctcccacgg gggcggcgga tacgacatgc gcaatatcct caaaggcact
     1741 ctgctggaag cgtgccccga cagcgccgtc acctttgtgg acaaccacga cacccagccg
     1801 gggcaggcac tgcagagctg ggtggacggc tggttcaagc cgttggccta tgcgctgatc
     1861 ctgctgcggc agcaggggtt gccctgcgtc ttttacggcg attattacgg catcccacac
     1921 gacaacatca acccggtggg cgggccgctg ctggccctgc tgcgggtgcg ggcggagcgc
     1981 gcgttcggcc cccagcacga ctatttcgat gaccccaacg tcatcggctg gacgcgggag
     2041 ggggacgcgg agcatcccgg cagcggcctg gcggccgtgc tctccgacgg cgcgggcggc
     2101 cccaagcgga tgtatgtggg ccagcgccat gcgggtgcca cctttgcgcc ggtgaccggc
     2161 ggcggcacgg cggtgctgat cagcccggac ggctggggcg atttttcagt ggacggcggc
     2221 aatgcgaggg tctatgtgcg gatctgaaaa gatttgatac aaaaatattt gaaagcgttt
     2281 ttatccggat ggggctggag gattccttgc cttccttttc ggtcaatgtt aagataaaaa
     2341 tataaaagaa aaagatgggg gaatcaagga tgaaagctcg tccccggctt tactgggcat
     2401 ttaatatatg ctttggcgtg tttttcatcg gtttgtgcat cgttagcatt ttttggcacg
     2461 ataatccgtt tgtgcagatg aagaaaagcc tgttgctagg aacaaccgtt acttttttgg
     2521 tggttgcgtt gggtgccgtg atcttatggc aacgtattgc gtggcctttg caccatgaaa
     2581 taagaacaat tgttgtgctg atgacgctct atcttttgct acagttgctg tttggatggc
     2641 aaatgcaagt ggggtttgac gctgaatggg attatcctat agtggcaaaa gctgctgagg
     2701 aactggtact gcatggtgtc cctccgggcg aatattttgc gcttttttct aataacggtc
     2761 ctcttttctg gttttatacc ggatattttc atctgctata ttggttcggg agccaagatt
     2821 ttgtgtacgg attgattata ttgaacagcc tgatgatcga cctctcgttc ttttttctct
     2881 atctgacggc gcgacagctg tgggggaaaa gaggagcatt gccgctactg ggggcggcaa
     2941 tgctctgtcc cgcttttttg ctgtatgtcc ctatcgccta cacagatacg ctcacattgc
     3001 cttttgtgac aggggcagtt tggctctggc tctgtgcgcg tagccgtcgc atggagggat
     3061 tgcccagcgg tcgctgggtg gcgggtgcgt tggcactgac ggctctgggc gcggtgctaa
     3121 aaatgtctgt ggctgtgttg gcttttgctt ttgcgctgga cttacttgtg tggtgggatg
     3181 gccgtggcag gctgcgtctc ttggcaatag gtgcggcctg ctctgcgacg ctctggacag
     3241 gggtgaattt tgccagtagg gccgctatgc ctccatttga ggagaagagt gttcctttta
     3301 cacattggat tatgatgggg ctgcacggca atgggggcta ctgggaccct gactatgaat
     3361 taaccctaca gtatgatacc tacgaagaac ggctcgtttt tacacaagga gagattttgc
     3421 ggcggttaaa agagatggga acagaggggc tggcaaaaca ttgtgtggct aagttgagct
     3481 atatcatcag tgatggtacc tgctatgcgc cggaaaagct ggatcggaat caccggggcg
     3541 gagaggcgtt gaatgatttt atcttgaaag gagggaaata ttcttccttt ttatattatg
     3601 gagcagacgg gctacagctt tgcctgctag ccctgtgtgc tgtgggaagc tttttaagcc
     3661 tcccgcggca gcagcaacaa gttacagtat ttcgcatagg agtctttgga ttgctgttgt
     3721 ttttgctctt gtgggaagca cgtagtagat atctggtcaa ttttttacct ctgttattac
     3781 tttgtgccgc cgacggtctg gcttctctct ataaaaggca aaggcactcc ccggatggaa
     3841 tatcgagctc ccgcaaagag atgagaaaat gggacttaaa tatccgataa ggtaggccaa
     3901 aacacagaag agaagccata aatggcccct gtatactcag ctgggaacaa agtggaattt
     3961 tcgattcaag tggaaaaaac aaacaggacg tccgtgtgat agcccggacg ccctgtttgc
     4021 agcttaaaaa gatcagacgg taaaaacctt attccacctc tgtgacccag ccctgggggg
     4081 cgggcaggcg gccgtactgg atgcccgtga gggtgtcgta gagccgctga gtgaccgggc
     4141 cgatgcggcc gccgttgatg accgcgacct ggtctttata cttcagctcg cccaccgggc
     4201 tgatgaccgc ggcggtgccg gtgccgaaga cctcctccag cgtgccctcc cggtcggccc
     4261 tcatcacctc gtcgatgggc agacgatcca ccttgacccg gtagccccac ccctctagca
     4321 gctcgatgca gctcatgcgg gtgatgcccg gcagcacggt gcccgtcgtg ggggcggtgt
     4381 ggatcacgcc gtccatcttg aaaaagcagt tcatgctgcc cacctcctcc acgtaccggc
     4441 gctccacccc gtccagccag agcacctggc cgtagcccag ccgatgggcc ttttcacctg
     4501 acagcaggct ggccgcgtag ttgccgccgc atttgacggc gccggtgccg ccgggggcgg
     4561 cgcgcacata ttcgtcctcc acatagatgc gcaccgggtc gatgccggtg gggtaatagc
     4621 tgcccgacgg gctggcgatg atgcagaatt ggtaggcgcc gctggctttg acccccagct
     4681 gcgcctcggt ggcgatggtg aaagggcgga tataaaggct ggtgccctcc tccgacggca
     4741 cccagtccct gtccgcgcgg accagcgctt tcaccgcctg cacgaaatcc tccaccggga
     4801 tcggcgggat gcagaggcgc tcatgggtgc tgcgcagtcg ctcgccgttt ttgtccgggc
     4861 ggaacagctg cacccgccca tctgcggtgc ggtaggcttt catgccctca aagcactcct
     4921 gcgcgtagtg aaagacctgc gcggccgggt ccagcacgat gggggcgtag ggcacgaccc
     4981 gcgggtcatg ccagcccctg tccgcggtgt aatccatcag gaacatgtgg tcggtgaaga
     5041 tggacccgaa gcccagcggg ccctcgggct tcggcttggg gtgagcggtc ctctcgatct
     5101 tgatctccag catggttcat ctctcgcttc cctctgttaa agttgcgggg caggggcact
     5161 ctccctggtc gggaagggcg cccttgcccc gcgctgtttt tatcatacgc cgaaacgtcc
     5221 cggtcttgca agccgcaaag agaccaaaat ttgggccgca gggccgtgaa aaaaggtgga
     5281 gtatgacggg cccccaaaaa gcgccgggca cggcccttta ctgccggccg gggccccggt
     5341 ctcccgcggc gggggggata tccgccggcg gctgtgtggc ggccgggcgc tttgcccgcc
     5401 gccgtcgggc ggcgcgcagc gagagcagca ccgcggtgag gaccacggcg atgagcagca
     5461 gcagcggcag cagatagatg aacaggatca ccagctcctg cgccaattcc accacgttgt
     5521 cgagggagcc ctgaaacgcg cgcaggatgc ggtcgccgaa agtgggggcg gtgatggtct
     5581 cccttgtcac ctcgttgacg atgaccgaga cggtggcgta ttccacctgg ttgtcataga
     5641 gcttctgctg gccggtgagg ctctcgatct ggtagcgcac ctcggtgagc ttatcctcca
     5701 gtgtgatcag atcctccagc ttcgccgcct gggccatcag ctccaacagg cgctgttcct
     5761 gggtgcgcag gctggcaagg cgcgcctcgt tgtccacgta ttcggccgtg acgtcggtgg
     5821 tgctctggct ggaggagaca acggcgccgg ccttgcccgc gctgtccaaa aaagaggaaa
     5881 gcttttcagc ggggatgcgg gcctcatagc gggcgctgcg gttggcggtg ccggcggcgc
     5941 cgtcctggtt gctgctggcg agatagccgc ccagcgctgt gacctggcgc tcgatctccg
     6001 ccgcggccgc ctcaaagtcg gtggcgtcca gccagagctc ggcattcacg atgagcttgc
     6061 ggtcgctctg ggcctttaca tcggtcaggg aggcgtcgta attttctgtc tccccgccgg
     6121 tcgcctgctc cgccgcggcg ggcgctgcgg aaaaatgatc ggctgcagtg gagcctccgc
     6181 aggcggccag ggcacagatg agggcgaggc agatcgaccc cgccgccagg cgggtgatga
     6241 gatgagggtg cttcatgagg agacctcctt taaagatggt tggagagcgg gcgtccccgc
     6301 gccgcggagg accgcccacg gatgccgcaa aacacttttt tacattctac aataagagac
     6361 ggaaaaagag cggttttttt acagcttttt gaaaaattga cggcaatttg taatctatgg
     6421 gcctgcgccg ccgcccgccc cacccgcacc ccgccgggcg gccggtggga gcctgcggaa
     6481 gtgtggtctt aaaaaggaag tgcggacagg aaaagcaacg cttttgaccg ccgggtcgcc
     6541 gcgcgccttg acagcaaagc cgggcgggcg gtatgatgac agaaagcagg acaaaccggc
     6601 ggcggccgga agaaacggag gatgaaaatg gacaaacagg cgatgctgaa ctggctggag
     6661 gggcaggagg gaatgctgtc caagatggcg cgggcgatct gggaaaagcc ggagctggca
     6721 atggaagagg tgtttgccag cggcctgcag cgggacgccc tggccgcggc gggctttcag
     6781 gtgcgggagg tgcccggggt gcccacggct tttgcggcgg aatacggcca cggcaagccg
     6841 gtgctgggca tcctggggga gtatgacgcc ctgccggacc tgtcccagca gcagggcccg
     6901 gagcggcgcg ccctggtgga gcacggcccc ggccacggct gcgggcacaa cctgctgggc
     6961 accgcgggcc tgggtgcggc catggcgctg gcaaaggcaa tcgagagcgg cgcgctggcg
     7021 ggcacggtgc ggtattacgg ctgcccggcc gaggagacca tcagcggcaa gaccctgatg
     7081 gccaaagccc acg
//
LOCUS       DBMDYA010000045         7038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2757_length_7038_cov_1.560040, whole genome shotgun sequence.
ACCESSION   DBMDYA010000045 DBMDYA010000000
VERSION     DBMDYA010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_7038_cov_1.560040"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(55..957)
                     /gene="dapA"
                     /locus_tag="ACZPFK_01960"
     CDS             complement(55..957)
                     /gene="dapA"
                     /locus_tag="ACZPFK_01960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1405106.1"
                     /translation="MKTCVFTGSCVALATPMNEDGSINLDVLKQLIEFEIENGTDAIL
                     VCGTSGEAPTLDQQEHMAVIRCAVKAVRGRVPLIAGTGSNDTRHAIAQSREAEALGAD
                     ALLLVTPYYNKTSQAGLVRHFTAIADSVSLPVILYNVPSRTGLNIQPETYRQLARHPR
                     IVATKEANGDIAALARTKQLCGDELWVYSGNDDQIVPVMSLGGVGVISVLANVAPALT
                     KEICAAGLAGDTARAAALQLKATPLISALFADVNPIPVKYALNRIGFAAGQCRLPLIA
                     PAAEVRRRVDEALEGLGLLAAPLS"
     gene            complement(981..2000)
                     /locus_tag="ACZPFK_01965"
     CDS             complement(981..2000)
                     /locus_tag="ACZPFK_01965"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1405107.1"
                     /translation="MKQYRIAVVGATGMVGRTFLKVLEEFDLPVSKYKLFASARSAGK
                     KLTLLGQEYTVEELTEQSFDEGFDLALFSAGAGISKKFAPIAAARGCVVIDNSSQWRM
                     DPKVPLVVPEVNPDDIKWHSGIIANPNCSTIQAMLPLKVLHERYGIRRVVYSTYQAVS
                     GAGVKGWDDLANGAKAFAEGKEAAELQKFPYPIFSNCIPHIDVFLEDGYTKEEHKMID
                     ETHKILGDDSIRITATTVRVPVFNGHSESINVELAKPYDLADVRRAMSEMEGIILYDD
                     VAHDIYPLASVACGRNEVYVGRLRRDDSVENGLNLWCVADNIRKGAAANAVQIARYMI
                     GHDMI"
     gene            2413..3441
                     /gene="queA"
                     /locus_tag="ACZPFK_01970"
     CDS             2413..3441
                     /gene="queA"
                     /locus_tag="ACZPFK_01970"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1405108.1"
                     /translation="MQKSDFYYELPKDRIAQEPAEPRDAARLMVLERDSGGISHHVFR
                     ELTTLLDDGDLLVVNNSKVLPARLLGVKEGTGAACEILILRPQGDDVWECLAKPGKRL
                     KAGAGVVFGDGVLTARILETTEEGNKIVSFTHPGRSFYEALDLVGRLPLPHYIEKEME
                     DDSEYQTVYAKEPGSAAAPTAGLHFTDRLLQELKQKGVQVAEVTLHVGLGTFRPVKVD
                     DITQHHMHTEWYSVSEETAALIRQTKAAGHRVIAVGTTSCRTLESVYRAHGEVCACQG
                     ETDIFMYPGFEFKVIDGLITNFHLPESTLIMLVSAFAGYDHTMAAYRTAVEQGYRFYS
                     FGDAMYIR"
     gene            complement(3703..4635)
                     /locus_tag="ACZPFK_01975"
     CDS             complement(3703..4635)
                     /locus_tag="ACZPFK_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1405109.1"
                     /translation="MDIRTLRYFLAVAREESITGAAAALHLTQPTLSRQLMELEAELG
                     KQLFLRGKRRVTLTEDGVLLRKRALEIVELMDKTEAELREPGGSVEGKVYIGAGETRA
                     MRLIAHVAKELQASHPLLRYHLFSGNADDVMERLDKGLLDFGVLIEPFDMKKYDCFQL
                     PVTDTWGVLMRRDDPLAERDSIRPEDLRGVPLFSSRRTMVKNELSKWFGAEFEALNIV
                     GTFNLIFNAALMVEEGLGYALCLDKLVGASGNEPLCFRPLEPRLEVGLDIVWKKYQMF
                     SKPAELFLEKLKKASGTASLPHLENTPGKRVLNL"
     gene            4979..5278
                     /locus_tag="ACZPFK_01980"
     CDS             4979..5278
                     /locus_tag="ACZPFK_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024924.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1405110.1"
                     /translation="MNTLYRRRFSHAHALSALSAEKQPGACLGCKSCEAVCPQQIKIS
                     QAMAAFAKSWITFLHGLLRKRIRAEDKDSRTAAVLVQALAVSVEAAPKAVKNESL"
     gene            complement(5439..5762)
                     /locus_tag="ACZPFK_01985"
     CDS             complement(5439..5762)
                     /locus_tag="ACZPFK_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405111.1"
                     /translation="MAEQKRIRRTSEQIAADIDVQIADQEEHIRALLAKKAAACQEFD
                     AKIAVVQKRITGLRGKKKSVLAPKKKKAHKSRAEQIKELVKCAQRSGMKLEEIADKLG
                     MDLSA"
     gene            complement(6065..>7038)
                     /locus_tag="ACZPFK_01990"
     CDS             complement(6065..>7038)
                     /locus_tag="ACZPFK_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1405112.1"
                     /translation="EPDHCANIGELLLRFPGLKIVGNSKTFAFIRQFYGLEINGRTLE
                     VDEGDRLPLGSHTLRFFLTPMVHWPEVMMTYEESERLLFSADAFGSFGALNGALFRDE
                     MDTQRDWLPEARRYYGNIVGKYGPQVQTALQRVSGVPLGMVCPLHGPVWRDGLQELLA
                     RYRRWCAYEPEERTVALLYGSMYGDTENAVNILASRLVDSGVRGIAVYDVSSTHVSTL
                     IAEVLRCSHLVLASPTYNNGVYPAMLNFLHDMQALNLQNRTVALIENGSWAPTSARRV
                     KELLAGMKQMRVLDPVVTIRSALEEGSYDTLMRLKKVILDSLSEVDK"
BASE COUNT         1459 a         2068 c         2061 g         1450 t
ORIGIN      
        1 cgtaatccgc gaggcagcgg cccatccggc ccagcgcgcc gttcagcaaa atcttcatga
       61 cagcggggcg gcgagcagcc ccaacccctc cagcgcctcg tccacccggc ggcggacctc
      121 ggcggccggg gcgatgaggg gcaggcggca ctgtcccgcc gcaaagccga tgcggttcag
      181 cgcgtatttt acggggatgg ggttcacgtc tgcgaaaagg gcggagatca ggggcgtggc
      241 tttcagctga agagcggccg cgcgggcggt gtccccggca aggccggcgg cacagatctc
      301 ctttgtcagc gcgggggcca cgttggcgag caccgagatg acccccaccc cgcccagcga
      361 catcaccggc acgatctggt cgtcgttgcc ggaataaacc cacagctcgt cgccgcagag
      421 ctgctttgtg cgggccagcg ccgcaatgtc gccgtttgcc tcttttgtcg ccacaatgcg
      481 cgggtgccgc gccagctggc gataggtctc gggctggata ttgagcccgg tgcgggaggg
      541 cacgttgtac aggatgaccg gcagcgagac gctgtccgcg atggcggtga agtggcgcac
      601 cagccctgcc tgcgaggtct tattataata aggtgtcaca agcagcagcg cgtccgcgcc
      661 aagggcctcc gcctcgcgcg attgggcgat ggcgtgccgg gtatcgttgg agccggtgcc
      721 cgcgatcagc ggcacccggc cgcgcaccgc ctttacggca cagcggatga cggccatgtg
      781 ctcctgttgg tcgagggtag gggcctcgcc gctggtgccg cacaccaaga tcgcgtcggt
      841 accgttttcg atctcgaatt cgatgagctg ctttaaaaca tccagattga ttgaaccgtc
      901 ctcattcatg ggcgtcgcca gggcgacgca cgatcctgtg aagacgcagg ttttcatgca
      961 gacctcccac tattcagttt ttagatcata tcgtgcccga tcatgtaccg cgcgatctgt
     1021 acggcgttgg cggccgcgcc cttgcggatg ttatccgcga cgcaccacag gttgaggccg
     1081 ttctccacgc tgtcgtcccg gcgcaggcgg cccacataga cctcgttgcg gccgcaggcg
     1141 acgctggcga gcgggtagat gtcgtgggcc acatcgtcat agaggatgat cccctccatc
     1201 tcggacatgg cgcggcgcac gtcggccaga tcgtagggct ttgccagctc cacgttgatg
     1261 gattcgctgt ggccgttgaa caccggcacc cgcacagtgg tcgcggtgat acggatcgag
     1321 tcgtctccca gaattttatg cgtctcgtcg atcatcttat gctcttcctt ggtgtagccg
     1381 tcctcgagaa agacgtcgat atgcgggatg cagttggaaa agatgggata ggggaacttc
     1441 tgcagctccg ccgcttcttt gccctccgca aaggctttgg cgccgtttgc cagatcgtcc
     1501 cagcccttga cccccgcgcc ggataccgcc tgataggtgg agtagaccac ccggcggatg
     1561 ccgtatcgct cgtgcagcac cttgaggggc agcatggcct ggatggtgga gcagttgggg
     1621 ttggcgatga tgccgctgtg ccatttgata tcgtcggggt tgacctccgg caccaccagc
     1681 ggcaccttgg ggtccatgcg ccactgcgag ctgttgtcga tgaccacgca gccgcgggcc
     1741 gcggcgatgg gggcaaactt tttgctgatc cccgcgcctg cggagaacag cgccaggtca
     1801 aagccctcgt caaagctctg ctcggtcagc tcctccaccg tgtactcctg ccccagcagg
     1861 gtcaactttt tgcctgcgct gcgcgcggag gcaaacaatt tgtattttga caccggcagg
     1921 tcgaactcct ccagcacctt gagaaaggtg cggcccacca tgccggtggc gcccactacg
     1981 gcgatccgat actgtttcat ctcacatact cccttttcca acgttttgac cctctctatc
     2041 gctgtctccg ccgcttttgt gcggcggggc gcgccaaaaa ataaaagcct ctgcccataa
     2101 gaaaagcggg cccaaacggg cccgtggggg cacagcttga aaaccgaagc ccgatgcaat
     2161 ataataatgt agccgcacag cccggcagcg gagtgggaca tgcgccggga gcggcgcttc
     2221 cgcccgccgc gctgcagagt ggggcaagcg gcatgccggc gccggctttt cttccctact
     2281 atagcatcgg gccgggggac gtgtcaatga aaaatgccgc aaaacaggca aaattgtcgt
     2341 ttttaacaga ataagtgtag ctgatgaaaa aacaaaattt ggatgttgaa acaggaaagg
     2401 tcgaaccaac agatgcagaa aagtgatttt tattatgagc tgccgaaaga tcggatcgcc
     2461 caggaaccgg ctgagccccg cgacgcggcc cggctgatgg tgctcgagcg cgacagcggc
     2521 gggatcagcc atcatgtgtt ccgcgagctg actaccctat tagatgacgg cgacctgctg
     2581 gttgtgaata actcgaaagt gctgcccgcc cggcttctgg gtgtcaagga gggcacgggg
     2641 gccgcctgcg agattttgat cctgcgcccg cagggggacg acgtgtggga atgcctggca
     2701 aaacccggca agcggctgaa agcgggcgct ggggtggtgt ttggagacgg cgtgctgacc
     2761 gcccgcatcc ttgagaccac ggaggagggg aacaagattg tctctttcac ccacccgggc
     2821 cgcagctttt acgaggcgct cgacctggtg ggccgcctgc ccttgcccca ctatatcgaa
     2881 aaagagatgg aggacgactc tgagtaccag accgtctacg ccaaggagcc gggcagcgcg
     2941 gcggccccca cggcgggcct gcattttacc gaccgcctgc tgcaggagct gaagcaaaag
     3001 ggcgtgcagg tggcggaggt cacgctgcac gtggggctgg gcacctttcg gcctgtaaag
     3061 gtggatgaca tcacgcagca tcacatgcac accgagtggt attccgtgtc ggaggagacc
     3121 gccgccctca tccggcagac caaagccgcg ggccaccgcg tgatcgcggt gggcaccacc
     3181 agctgccgca cgctggaatc cgtgtaccgg gcgcacggtg aggtatgcgc ctgccagggg
     3241 gagacggaca ttttcatgta tcccgggttc gagttcaagg tgatcgacgg cctcatcacc
     3301 aattttcatc tgcccgagag cacgctcatc atgctggtct ccgcctttgc gggctacgac
     3361 cacaccatgg ccgcctaccg cactgcggtg gagcagggct atcgctttta ttcctttggg
     3421 gatgcaatgt atatcagatg agcggatttt atcagcataa tcggcacaca aagaacccgc
     3481 aaaagctcag atcttctgcg cctttgcggg ttctttgtgt caattttact tctcttcggc
     3541 tgttttgggg ctggggccaa gccgccgggg atgtggacag agagaagacc gtaccaacgc
     3601 gacatccttc gtatgtcagc agccaaaggg ctttgcctgt cactcaaaaa tccacagctc
     3661 ggctggtgga tgctccttgg taaaaatctc gtcaacgctc tattaaaggt tcaaaacgcg
     3721 tttgccgggc gtgttctcca gatgtggaag tgatgcggtg cccgacgcct ttttgagctt
     3781 ttccaaaaac agctcagcgg gttttgaaaa catttgatac ttcttccaca caatatccaa
     3841 tccgacctcc agtctcggct ccagcgggcg gaagcagagg ggctcgttgc ccgaggcgcc
     3901 caccagcttg tcgaggcaaa gggcgtaccc caagccttct tccaccatca gcgcagcatt
     3961 gaagatcaaa ttgaaagtgc ccacaatgtt gagggcttca aactccgccc cgaaccactt
     4021 tgaaagctca ttttttacca tggtgcgccg ggaagaaaag agggggacgc ccctcaaatc
     4081 ctcgggacgg atgctgtccc gctccgccag gggatcgtcc ctgcgcatca gcacccccca
     4141 ggtatcggtg accggaagct ggaagcaatc gtattttttc atgtcaaagg gctcgatgag
     4201 cacgccgaaa tccagcagcc ccttgtccag ccgctccatc acgtcgtcgg cgttgccgct
     4261 gaacaggtga tagcgcaaaa gcggatggct ggcctgcagc tcttttgcca cgtgggcgat
     4321 caggcgcatt gcccgtgtct cgccggcgcc gatatagacc ttgccctcta cgcttccgcc
     4381 cggctcgcgg agctccgcct cggtcttgtc catcagctcc acaatctcca aagcgcgctt
     4441 gcgcagcaaa acgccgtcct cggtcagggt cacccggcgt ttgccgcgca ggaagagctg
     4501 cttgcccagc tcggcctcca gctccatcag ctggcgggac aaggtgggct gggtgagatg
     4561 aagcgcggcg gccgccccgg tgatgctctc ctcgcgggca acggccaaaa aatagcgcag
     4621 cgtgcggata tccatgcatt ttcctccccg cattttttct catcctatcc cttgaatcaa
     4681 tacctgtcaa gcatggaaat caatcagatg ggagcattta tggcgttgta agctctgtga
     4741 taaaattaaa atagaaacaa gtaaaaagca atcctctgcc gtcgagagag aaagggcgga
     4801 aacgcggcac ggaaaaggac agagcgacgt aatggggtag ggagagttgt ccgtcccgac
     4861 gcaagatggg gagaagagca aaaacattca ttttggccca gagacagcta ttggatagtg
     4921 gagaaaacgg gcaccgttcc cggcccgggc cgtggggaca gagcctcgac tcggaaaaat
     4981 gaacacgctt tacaggcggc ggttttccca cgcccatgcc ctgtccgccc tgtccgccga
     5041 aaagcagccg ggcgcctgcc tgggctgtaa aagctgcgag gcggtctgcc cgcagcagat
     5101 caaaatatcg caggccatgg cagcttttgc aaaaagctgg ataacctttc tacacggtct
     5161 tttgagaaaa aggatcagag cagaagacaa ggacagccgc accgcagctg tccttgttca
     5221 ggctttggcg gtctcggtcg aagcggcgcc aaaagcagta aagaacgaat cgctttgata
     5281 ggctgcacaa aaaaaccagg cacggatggc ttagggcgca gtgtgagccg agatggtttg
     5341 gccttgccct atcggaactg cgttgacatc catgcttggt ttttcatgtg aaatatctaa
     5401 actgaaagcg tttaaaagag atcacagcgc ttcatttttc aggcggataa gtccattccc
     5461 agcttatcgg cgatctcctc caatttcatc cccgatctct gcgcgcattt taccagctct
     5521 ttgatctgtt ccgccctgct cttgtgggct tttttctttt tgggggcaag aacagatttc
     5581 tttttgccgc gcaggccggt gatccgtttt tgtacgacgg cgatcttggc gtcgaattcc
     5641 tgacaggccg ctgccttttt tgccaacaaa gcccttatat gttcttcctg atctgcgatc
     5701 tgaacgtcga tatccgccgc aatctgctcc gacgttcttc tgatccgttt ttgttccgcc
     5761 ataggccaac gctccttttg taaatcaaaa tctgtatttt taatatagca cgaatgattt
     5821 gtaggaatcg caggatttct gacaaaactt ttttgcgctc agcgaaaatt tgatcccaaa
     5881 cccatgagct acttatgttt tttctctgcg gctcaaaacc tgccggcagg gcgaaagatt
     5941 gaaatttcgc cgtgcagccc ggggggcgac ggtaggcaaa tcagaaaaag cggaaagctc
     6001 ccgctgcggc acagcaagcg cagtccatct gttctgcttt ttaactgcaa agtaaaacgc
     6061 cttatcactt atcgacttca gagagggaat ctaaaatcac ctttttcagc ctcataagag
     6121 tgtcataaga cccctcttca agcgctgagc ggatggtcac caccgggtcc agcacccgca
     6181 tctgtttcat cccggccaaa agctctttta cgcgccgggc actggtgggc gcccaggagc
     6241 cgttttcaat cagggcgacc gtgcggtttt gcaggttcag cgcctgcata tcatgcaaaa
     6301 aattcagcat cgcaggatag acgccgttgt tgtaggtggg ggaggccaaa accagatggc
     6361 tgcagcggag tacctcggcg atcagggtgg agacgtgggt gctggagaca tcataaacag
     6421 cgatccccct gacgccgctg tccaccaggc gggaagccag gatgttgacg gcgttttccg
     6481 tgtccccgta catggagccg tacagcagcg ccacggtccg ctcctccggc tcataggcgc
     6541 accagcgacg atacctggcc agcagctctt gcagcccatc gcgccatacc ggcccgtgca
     6601 gcgggcagac catacccagc ggcacaccgg ataccctttg cagcgctgtt tgcacctggg
     6661 ggccgtactt tcccacgata ttgccgtagt agcggcgggc ttcgggcagc cagtcccgct
     6721 gggtatccat ctcatcgcgg aacagcgccc cgttcaaagc gccgaagctg ccgaacgcgt
     6781 cggcggaaaa aagaagccgt tcgctctctt cataggtcat catcacctcc ggccaatgca
     6841 ccatgggggt gaggaaaaaa cgcagcgtgt ggctgccgag cggcagccgg tccccctcgt
     6901 ctacctccag ggtgcggccg ttgatctcca gaccgtaaaa ttggcgaatg aaagcaaagg
     6961 tcttgctgtt gccgacgatt ttcaatccgg gaaagcgcag cagcagttca ccgatattgg
     7021 cgcagtggtc gggctcca
//
LOCUS       DBMDYA010000046         7034 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2759_length_7034_cov_1.724362, whole genome shotgun sequence.
ACCESSION   DBMDYA010000046 DBMDYA010000000
VERSION     DBMDYA010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_7034_cov_1.724362"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..1062)
                     /locus_tag="ACZPFK_01995"
     CDS             complement(61..1062)
                     /locus_tag="ACZPFK_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HIR1405113.1"
                     /translation="MEKTKSKLTKKSVVGFLSNNAITLLILLMVIYVGFSSDNFFTWS
                     NLKNLISNSSTRFIIACGVSGCLITKGTDLSAGRTVGLGACIAATLLQKVDYGSRFFP
                     NLPDFPVWAGLLFSMIVCGLFGVINGCVIAFLKVPAFIATLGMQTIVYGICLIYTGAD
                     PMGGFKSSYTVLGSGTFLGIPNLFFFALIVGLFIWFLYNKTRHGKYMYAIGGNESAAS
                     VAGVNVPATLIRIYFLASMLYGLAGFLVGAKTGGASVQTGYGYELEAIAACTIGGVST
                     NGGVGKVSGILIGVLVFEILKICLQFLGVDPSYTFIVQGLVIIVAVALDLRKYLAKK"
     gene            complement(1078..2589)
                     /locus_tag="ACZPFK_02000"
     CDS             complement(1078..2589)
                     /locus_tag="ACZPFK_02000"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405114.1"
                     /translation="MSEYRLEMRDVVKTFPGVKALDHAQLKLRPGTVHALMGENGAGK
                     STLMKCMFGIYKMDEGQIVYEGEPIQIKDPLDALNHGIAMVHQELQPIPERSVAENIF
                     VGRYPIKKLLGFYPIIDHKKMYEDTAKLLEEVRMPFDPRAKLGTLSVSQMQLVEIAKA
                     VSANCKVLILDEPTSSLTENEVEALFRIVDDLRAKGVSIVYISHKMDEILRISDEVTI
                     MRDGQYIGTWAAKELTTDKIITKMVGRELENLYPPRENVPSEVVFEVRDFTSINPKSF
                     RDVSFQLRRGEILGVGGLVGAQRTELMEGLFGIRAHAKGKIYYKGEEIHVSRPRDAIK
                     KGLAMLTEDRRATGIMGVLSVADNVAIASLNEYVDAGIVLNRKKIEKLVADNVAKLSI
                     KTPSSKTLIQSLSGGNQQKVLISRWLANDPDVFILDEPTRGIDVGAKYEIYCIIADLA
                     KQGKSIIMISSEMGELIGMSDRVMVMCDGKVTGFIDGDKANQENIMELATKFS"
     gene            complement(2678..3721)
                     /locus_tag="ACZPFK_02005"
     CDS             complement(2678..3721)
                     /locus_tag="ACZPFK_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1405115.1"
                     /translation="MKKVLALVMAAALALSLVACGGSDSSSSTPASQAASSAAGSIAS
                     TVPAGGKVGICIYKFDDNFMTVYRNKLEEILTGKGYEVSVVDGNNDQAKQTEQISTFI
                     TQGVDALIINPVMTSAAEQIVSMVKDANIPTVLINREPTEGEMAVYDKIAYVGCDAAQ
                     SGTFQGELILETEKKGDINGDGVVSYIMIQGDPENVDAKLRTEYSVKALEDAGVKVEQ
                     LDMQRGDWDQEKGQTITANALAQYGDKLEVVFCNNDAMALGALEAIKAANRTVGKDIY
                     LVGVDALDAALDAVSAGEMTGTVLNDANSQAAKAVEVMEALLGGKTYTADEQQIYVDY
                     VKVTKDNVADFMG"
     gene            complement(4610..5386)
                     /locus_tag="ACZPFK_02010"
     CDS             complement(4610..5386)
                     /locus_tag="ACZPFK_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1405116.1"
                     /translation="MREETALYRALYQSILTQIYSGVIRRGQRFPSQRQICAQYNVGI
                     TTVRKAIRMLEEEGVIDAGPGRRATVRFDAHSEACARALARRADAIRSAYIALQILAV
                     SGGRLALAAAQGLPAAACGAIAAQWEGMWGEALPHQAVQLLLGFLKSQAPGACLKEIL
                     GQLDALLIWGYYLKRLPRDPRAPALEEELGRHFREAAALLRRGEAGAFAGQAQAVLRC
                     AYRFARRQLAALVPDPARLPAALEGPQSPRATGAGRMGNK"
     gene            5589..>7034
                     /locus_tag="ACZPFK_02015"
     CDS             5589..>7034
                     /locus_tag="ACZPFK_02015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024818.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405117.1"
                     /translation="MNMLRVSVSKHLLNDHFTLVWANPFYYELIGYSREEYEALYHGQ
                     CDRYYVNEALGVDDGEEWARIGAEVRRALSEGRPGYTLTSRMRRKNGDYLWVQMSATF
                     VNEYQDGCQLSYTVMTDITETMQRQIEQSVAYDNLPGFVAKYRVSPGPQFTLLDANAG
                     FDAFFGGEWREMEGQSPLFCRNLRRNADVLAACGGDLLAGRPVHFTVHAEDQKGRSVW
                     LQVNGACVDHQGPAPVYLAIFIDITDETELRRMQEKLELQAEQLQQALRLAERASRAK
                     SDFLSHMSHDIRTPMNAIVGMTDIAKIHLNDPEKVKGCLDKITLSSRHLLGLINDVLD
                     MSKIENGSIALNPQPLSLAELLENVVTILQPSIEARRQNFSVQLQEVLHERYRSDALR
                     LRQIFINLLSNASKFTPPGGSVTLELEERPGPDAAHATLRAVVSDTGIGMSPAYLERL
                     FDPFSREEDTRTTVTEGTGLGMAITKRLVDLL"
BASE COUNT         1381 a         2140 c         2097 g         1416 t
ORIGIN      
        1 ccaccggggc gtcgccggta ccctgttctg aaaggcggtc gtctctctct tccatgcaga
       61 tcacttcttg gccagatatt tgcgcaggtc aagggcgacg gccacgatga tgacgaggcc
      121 ctgcacgata aaggtatacg agggatcgac gcccaaaaac tgcaggcaga tcttcaaaat
      181 ctcaaagacc aaaacgccga tcaggatgcc ggacaccttg cccacgccgc cgttggtgga
      241 aacgccgccg atggtgcagg ccgcgatggc ctccagctca tagccgtaac cggtctgcac
      301 cgaggcgccg ccggtcttgg cgcccaccag aaagccggcc aggccgtaga gcatcgatgc
      361 caggaagtag atgcggatca gcgtggcggg cacgttgaca ccggccaccg aggccgcgct
      421 ctcgttgccg ccgatggcgt acatgtattt gccgtgacgg gtcttattgt acaagaacca
      481 gatgaacagg cccacgatca gggcaaaaaa gaacaggttg gggatgccga gaaaggtgcc
      541 gctgcccagc acggtatagc tggacttgaa gccgcccatg gggtccgcgc cggtatagat
      601 caggcagatg ccgtagacga tggtctgcat gcccagcgtg gcgataaagg ccggcacctt
      661 taaaaaggcg atgacgcagc cgttgatgac gccgaacagg ccgcagacga tcatggagaa
      721 cagcagtccg gcccagaccg ggaaatccgg caggttgggg aagaagcggg agccatagtc
      781 caccttttgc agcagcgtgg cggcaatgca ggcgcccagg cccacggtgc ggccggcgga
      841 caggtcggtg cccttggtga tcaggcagcc ggagacgccg caggcgatga tgaagcgggt
      901 cgaagagttg gagatcaggt ttttcaagtt gctccaggta aaaaagttgt cgctgctgaa
      961 gcccacataa atgaccatca gcaggatcag cagagtgatg gcgttattgg acagaaaacc
     1021 tacgaccgac tttttcgtca gcttgctttt tgttttttcc attactgtga ttcctcctta
     1081 cgaaaacttt gtggccagtt ccatgatgtt ctcctggttg gctttgtcgc cgtcgataaa
     1141 gccggtgacc ttgccgtcgc acatgaccat cacgcggtcg gacataccga tgagctcgcc
     1201 catttcggag gagatcatga tgatgctctt gccctgtttt gccaggtcgg cgatgatgca
     1261 gtagatctca tattttgcgc cgacatcaat gccgcgggtc ggctcgtcta ggataaaaac
     1321 gtccgggtca ttggcgagcc agcggctgat cagcaccttt tgctggttgc cgccggaaag
     1381 cgactggatc aaagtcttgc tggagggggt cttgatggag agcttggcga cgttgtccgc
     1441 caccagcttt tcgatctttt tccggttgag aacgatcccc gcgtccacat actcgttgag
     1501 ggaggcgatg gccacgttgt ccgccaccga gagcaccccc atgatgccgg tggcgcggcg
     1561 gtcctcggtc agcatcgcga ggcccttttt gatggcgtcg cgggggcggc tgacgtggat
     1621 ctcctcgccc ttgtaataga ttttgccttt cgcgtgcgcg cggatgccga acaggccctc
     1681 catcagctcg gtgcgctgtg cgcccaccag gccgcccacg cccaaaatct cgccccgccg
     1741 cagctgaaag ctgacatccc ggaaagactt ggggttgatg gaggtgaagt cccgcacctc
     1801 aaagaccacc tcgctgggca cgttctcccg cgggggatag aggttttcca gctcgcggcc
     1861 caccatcttg gtgatgatct tgtccgtggt cagctctttt gccgcccagg tgccgatgta
     1921 ctggccgtcg cgcatgatgg tgacctcgtc cgagatgcgc aggatctcgt ccatcttgtg
     1981 ggagatgtac acgatcgaga cgccctttgc gcgcaggtcg tccacgatgc ggaacagcgc
     2041 ctccacctcg ttctcggtca aagaggaggt gggctcgtcc aaaatcagca ccttgcagtt
     2101 ggcgctgacc gccttggcga tctccaccag ctgcatctgg gagacggaga gagtacccag
     2161 cttcgcccgc gggtcaaagg gcatgcggac ctcttcaagc agcttggcgg tgtcctcgta
     2221 cattttttta tggtcgatga tcggatagaa ccccagcagt tttttgatcg gatagcggcc
     2281 cacgaagata ttttccgcca cgctgcgctc cgggatgggc tgcagctcct ggtgcaccat
     2341 cgcgatgccg tggttgagcg cgtccagcgg gtccttgatc tggatgggct cgccctcgta
     2401 gacgatctgc ccctcgtcca tcttgtagat accgaacata cacttcatca gcgtggattt
     2461 gcccgcgccg ttctcgccca tcagtgcatg cacggtgccc ggtctcagtt tcagctgcgc
     2521 gtggtccagg gccttgacgc ccgggaaggt cttgaccaca tcgcgcatct ccaaacgata
     2581 ctccgacatt aactggcccc cttgcattta taatcccaaa aagtgcgtgt gcgtcccagc
     2641 gcacacgcac tctctaattc ttttggtcta tcgaagctca gcccatgaag tcggcgacat
     2701 tgtccttggt gaccttgaca tagtcgacat agatctgctg ctcgtcggcg gtgtaggtct
     2761 tgccgcccag cagggcctcc atgacctcaa cggccttggc cgcctgggag ttggcgtcgt
     2821 tcagcacggt gccggtcatc tcgccggcgg agacagcgtc cagagcggcg tccagagcgt
     2881 cgacgcccac caggtagatg tccttgccca cggtgcggtt cgcagccttg atggcctcga
     2941 gagcgcccag agccatggcg tcgttgttgc agaagacaac ctccagctta tcgccgtact
     3001 gcgccagagc gttggcggtg atggtctggc ctttctcctg atcccagtcg ccgcgctgca
     3061 tatccagctg ctcgaccttc acgccggcat cctccagcgc cttgacggag tactcggtgc
     3121 gcagcttggc atccacgttc tcggggtcgc cctggatcat gatgtaggag acaacgccgt
     3181 cgccgttgat gtcgcccttt ttctcggtct caaggatcag ctcgccctgg aacgtgccgg
     3241 actgggcggc gtcgcagccg acataagcga tcttgtcgta gacggccatc tcgccctcgg
     3301 tgggttcgcg gttgatgagg acggtgggga tgttcgcatc cttgaccatc gagacgatct
     3361 gctcggcggc agaggtcatg accgggttga tgatcagcgc gtcgacgccc tgggtgataa
     3421 aggtgctgat ctgctcggtc tgcttggcct ggtcgttgtt gccgtcgacc acggagacct
     3481 catagccctt gccggtgagg atctcctcca gcttgttgcg gtagacggtc atgaagttgt
     3541 cgtcaaactt gtagatgcag atgccgacct tgccgccagc ggggacggtg gaggcaatgc
     3601 tgccggccgc ggaggacgca gcctgggaag ccggggtgga agaactgctg tcgctgccgc
     3661 cgcaggcgac cagggacagg gccagcgcag cggccatgac cagtgcgagt acttttttca
     3721 ttggggttct ctctcctttt tcattcaacg ccattcaaaa cccgcattcg cgcccggccc
     3781 agacccaacc cttacccggc cgcctctccc gcccctgtgg acaagagaag cagatatgta
     3841 aatttgttgt ctgaaacctg gacatttttt gcagattttg aacagaattc cttttgtacc
     3901 attccgtttc ggtacgccta aatggtagca tccccctgtg ggcatgtcaa caaaaatgtg
     3961 agttttgata cggtttttta attaataacg cccgtatttg gtagaaacag caaaaacttt
     4021 aagctttttt tgtggataaa ccataaaatg acactcatat atttttattt caaaaaatga
     4081 atttttgttg catatctaaa aaatgttgcc aaaaaaagtt gaaattttgc gtcccggttc
     4141 cacagcggcg gtaaaaaagt tttttgccgg tgaaaagagg tctgatagga cgagaggcgg
     4201 gggccgaaag acggcgttcc tccctcccgg cggcaaaaag gtcagctttg aaagcaccgc
     4261 ccagagggcg cgcttgcgca aaaaaagcat tgtctctgag agggggaaat ctgcgcgcaa
     4321 agcgccgtca aatcctcccc gcaaccgcgg agggcttcgt ggctgcaccc tgtcaaccgc
     4381 ctgagtgaac agacgacctg cgctaatttt tttagctccg cgccgcagcc ctccgggggc
     4441 gcgcggcagt ttcatacaga cggtttcgac gctccgtaaa ttgaaaaagg ttggcaatct
     4501 ccgcggattg ccaacctttt ttacgctatc atctcttcaa aaccatgggg ccgaaaccca
     4561 tactctctca ccacaacgcg ccaaagccgg tctctccctt tttctgttct catttatttc
     4621 ccatccggcc ggcccctgtc gctcgggggc tctgcggccc ctccagcgca gcgggcagac
     4681 gggccgggtc ggggacaagc gcggcgagct gcctgcgcgc aaaccgatag gcgcagcgca
     4741 gcaccgcctg ggcttgcccg gcaaaggccc ctgcctcccc gcgccgcagc agcgcggcgg
     4801 cctcacgaaa atgccgcccc agctcttctt ccagcgccgg ggcccgcggg tcccgcggca
     4861 gccgcttcag gtaatagccc catatcagca gggcgtccag ctgccccaaa atctctttca
     4921 gacaggcgcc cggggcctgg cttttcaaaa aacccagcag cagctgtacc gcctggtggg
     4981 gcagagcttc gccccacatg ccctcccact gcgccgcaat cgcgccgcac gcggcggccg
     5041 ggaggccctg cgcggcggcg agggccaggc ggccgccgct gacggccaat atctgcagcg
     5101 cgatgtaggc gctgcggatg gcgtccgccc gccgcgccag ggcccgcgca caggcctcgc
     5161 tgtgcgcgtc aaagcgcacc gtcgcccgcc gccccggccc ggcgtcgatc acgccctcct
     5221 cctccagcat gcggatggcc tttcgcacgg tggtgatgcc cacattatat tgggcgcaga
     5281 tctgcctttg cgaggggaac cgctgcccgc ggcggatgac gccgctgtag atctgggtca
     5341 ggatggattg ataaagcgcc cggtacaggg cggtctcctc cctcatcttt tcccctccca
     5401 tcggcgtttt tccactgagc cgagtataac acaaatagaa aaggctgaaa acacttgccc
     5461 attcaggata taaggtttaa actatacctg tagctaattc gagaaagatt aacaggccgg
     5521 gcgttggtcc ggagaggacg gcgagggaca cggaaaatta caggacggac tacgagtaca
     5581 cgaccctgat gaacatgctg cgggtgagcg tcagcaagca tctgctgaac gaccacttca
     5641 cgctggtgtg ggccaatccg ttttattacg agctgatcgg ctactcccgc gaggaatacg
     5701 aggcgcttta tcacggtcag tgcgaccgct attatgtcaa cgaggcgctg ggggtggacg
     5761 acggggagga gtgggcgcgc atcggcgccg aggtgcggcg ggcgctgagc gaaggccggc
     5821 ctgggtatac cctcacctcc cgcatgcgcc gcaaaaacgg ggactatctc tgggtacaga
     5881 tgtcggccac ctttgtgaac gagtatcagg acggctgcca gctgtcctat accgtcatga
     5941 ccgacatcac cgagaccatg cagcggcaga tcgagcagtc ggtggcctat gacaatctgc
     6001 ccggtttcgt cgccaaatac cgggtcagcc cggggccgca gttcaccctg ctggacgcca
     6061 acgcgggctt tgacgccttt tttggggggg agtggcgcga gatggagggg cagagccccc
     6121 tgttctgccg aaacctgcgg cgcaacgcgg atgtgctggc cgcttgtggc ggcgacctgc
     6181 tggcggggcg gccggttcac ttcaccgtgc acgccgagga ccagaagggc agaagcgtct
     6241 ggctgcaggt caacggcgcc tgcgtggacc accaggggcc cgccccggtt tatctcgcca
     6301 tcttcatcga catcaccgac gagaccgagc tgcgccgcat gcaggaaaag ctggagcttc
     6361 aggccgagca gcttcagcag gcgctgcggc tggccgagcg ggccagccgg gcaaaatccg
     6421 actttctttc ccacatgtcc cacgacatcc gcacccccat gaacgccatc gtcggtatga
     6481 ccgacattgc caaaatacac ctaaacgacc ccgaaaaggt aaaaggctgc ctcgataaga
     6541 tcacgctgtc cagccgccat ctgctgggcc tgatcaacga cgtgctggac atgagcaaga
     6601 tcgaaaacgg cagtatcgcg ctcaacccgc agcccctctc gctggcagag ctgctggaaa
     6661 acgtggtgac catcctgcag ccgagcatcg aggcgcggcg gcagaatttt tcggtgcagc
     6721 tgcaggaggt gctgcatgag cgctatcgct cggacgcgct gcgcctgcgc caaattttta
     6781 tcaatctgct gtccaacgcc tccaagttta cgccgccggg cggctcggtg acgctggagc
     6841 tggaagagcg gccgggcccg gacgccgccc acgccacgct gcgcgccgtg gtctccgaca
     6901 ccggcatcgg catgagcccg gcttatctcg agcgcctctt tgacccgttc agccgcgagg
     6961 aggacacccg caccaccgtc accgagggca ccggcctggg catggccatc acaaagcggc
     7021 tggtggacct gctg
//
LOCUS       DBMDYA010000047         6879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2826_length_6879_cov_1.692977, whole genome shotgun sequence.
ACCESSION   DBMDYA010000047 DBMDYA010000000
VERSION     DBMDYA010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_6879_cov_1.692977"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="ACZPFK_02020"
     CDS             <1..718
                     /locus_tag="ACZPFK_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1405118.1"
                     /translation="KKEPTKGNAMMFLMDMFLLFAVGSVLGVAVETVYVYLATGRLEN
                     RRGMVYGPFNQVYGLGAVLLTALLAPLAGESRLALFLGSAVLGGLFEALCSFFQEKAY
                     GTVSWEYSGQRLSLLGGRTSVTYMLFWGVLGSIYIRVLHPWLFGLFGKMPPPVKVPVV
                     LALTAFLAYDLWLSNAAVRRWNQRLREAPAAGEWDLWLDMKFPDARMRRLYPSMVPAS
                     HPEKPKRPRKTRKKAPVGTE"
     gene            791..1231
                     /locus_tag="ACZPFK_02025"
     CDS             791..1231
                     /locus_tag="ACZPFK_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1405119.1"
                     /translation="MLIFQNSLIYAPLLSYLVTQALKVAIYSYKDGRFDYKRFFSSGG
                     MPSSHAATVSALALAAWRQLGPLSPVTALALVVALIVMYDARGVRQETGKHAKILNEL
                     SENLWGADTESRNKKLNETVGHTLLQVLVGGMIGALIAFLVPLN"
     gene            complement(1674..2009)
                     /locus_tag="ACZPFK_02030"
     CDS             complement(1674..2009)
                     /locus_tag="ACZPFK_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1405120.1"
                     /translation="MEAKTLPACPVETTLTLIGDKWKVLILRDLMGGTKRFGELKRSL
                     GQVSQKVLTAQLRAMEESGLLLRKVYAEVPPRVEYTLTETGYSLKPILDAMWSWGEDY
                     KKKTASPPA"
     gene            2158..2559
                     /locus_tag="ACZPFK_02035"
     CDS             2158..2559
                     /locus_tag="ACZPFK_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1405121.1"
                     /translation="MNEVVKFLTENPVQYLATVGRDGKAKCRPFMFAGEKDGKLWFCT
                     NNTKDVYKDLQANPYTEVSVSSPAYAWIRLNGKAVFEDNMAVKEMCIQNPIVKGQYQT
                     ADNPIFEVFYLDGAHAVIADFSGNPPQEFDL"
     gene            2836..3459
                     /locus_tag="ACZPFK_02040"
     CDS             2836..3459
                     /locus_tag="ACZPFK_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1405122.1"
                     /translation="MTTVEILKMLQRDIHSAVFATVDENGLPQTCVIDLMLADEGGLY
                     FLTARGKAFYRRLTAAGFVALSGMKGEGTLSTVAISVRGPVRNLGSARLDEIFKENPY
                     MARIYPTAASRLALEVFQLYRGEGEYFDLSQQPPFRQAFSFGGQQAAPVGYCIDGARC
                     IGCQACLLSCPSGCIRDAVPRSIDASHCLHCGNCLRACPVGAVTRLG"
     gene            3480..4286
                     /locus_tag="ACZPFK_02045"
     CDS             3480..4286
                     /locus_tag="ACZPFK_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HIR1405123.1"
                     /translation="MNRLEQLQRLNSMLLEEMPEYGAQAAAFPPEEGAQRRLLRSLMN
                     LRPPLPLRADFLQLQDALLSAERVERGVVEVSSLPLTAADPRLCLWRGDITRLAADAV
                     VNAANSALLGCFIPCHGCIDNAIHSAAGLQLRDACFQLMRAQGHEEPVGSAKITPGYN
                     LPARFVLHTVGPIVNGPLEARHRAQLASCYRACLALAAERGLESVAFCCISTGEFHFP
                     APEAAQIAVGAVQEFLREKTSIRKVIFNVFQERDADLYRALLGPDRAPEG"
     gene            4216..5088
                     /locus_tag="ACZPFK_02050"
     CDS             4216..5088
                     /locus_tag="ACZPFK_02050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR1405124.1"
                     /translation="MSFKRETPISTAPCSARIARLRAELQEADAVVVGAGSGLSASAG
                     LTYSGERFEKNFADFIAEYHFRDMYSAGFYPYPTPEEYWAYWSRHIYCNRYDRAPGQP
                     YADLLALLEGKDYFVVTTNVDHCFQSAGFDKRRLFYTQGDYGLWQCSLPCHQKTYDNE
                     AAVRRMIAEQRRMRVPSELVPHCPVCGRPMAMNLRCDESFVQDEGWYAARDRYEDFLR
                     CHQGLRVLYLELGVGGNTPVIIKYPFWRLTAQNPNAFYVCVNLGEAAAPRELAARSLC
                     IDGDIGAVLARLRG"
     gene            5598..5768
                     /locus_tag="ACZPFK_02055"
     CDS             5598..5768
                     /locus_tag="ACZPFK_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405125.1"
                     /translation="MFCENLKNIRRAKGISQELLAEQLHVVRQTVSKWENGFSVPDQT
                     PLSGWPSFLKPL"
     gene            6291..6686
                     /locus_tag="ACZPFK_02060"
     CDS             6291..6686
                     /locus_tag="ACZPFK_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1405126.1"
                     /translation="MVSFDQFVLQDGVPLYLQIVRYLQRGLVSGAIPPGAELPSRRVL
                     SALLGVNPNTVQKAYRLLEDSGVIASHTGAKSFVCAGEDTIARIRAQLLESDVKSAVE
                     ALRQSGLSKSEALALVERFWDQPSGKEQV"
     gene            6683..>6879
                     /locus_tag="ACZPFK_02065"
     CDS             6683..>6879
                     /locus_tag="ACZPFK_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405127.1"
                     /translation="MKRHLSALMLALRSTIYRAAALAILAGAAQAALFLFAARRLQGE
                     AAPGLEEVFAASFAPQGCAVF"
BASE COUNT         1283 a         2111 c         2058 g         1427 t
ORIGIN      
        1 aaaaaaggaa ccgacgaaag gaaacgccat gatgtttttg atggatatgt ttctcctgtt
       61 tgcggtgggc tccgtgctcg gcgtggcggt ggagaccgtc tacgtctatc tcgccaccgg
      121 gcggctggag aaccgccgcg gcatggtcta cgggccgttt aatcaggtgt acggccttgg
      181 cgcggtcctg ctgacggcgc tgctggcccc tctggccggg gagagcagac tggcgctgtt
      241 tctcggcagc gcggtgctgg gcgggctttt cgaggcgctg tgcagctttt tccaggaaaa
      301 ggcttacggc acggtgtcct gggaatattc cggccagcgg ctctccctgt tgggcggccg
      361 gaccagcgtc acctatatgc tcttttgggg cgttttgggc tcgatctata ttcgggtact
      421 tcatccgtgg ctgtttgggc tgttcggcaa aatgcctccg cccgtgaaag taccggtggt
      481 gctggcgctg accgcgtttt tggcctatga cctctggctt tccaatgcgg cggtgcggcg
      541 ctggaatcag cggctgagag aagcccccgc tgccggtgag tgggatctct ggctggacat
      601 gaaattcccc gatgcccgga tgcgtcggct ctaccccagc atggtgcccg cgagccaccc
      661 ggaaaagccg aagcgtcccc gcaaaacgcg caaaaaggcg cctgtcggca ccgagtgaca
      721 ggcgcttctg tgggacgccg gagtattcaa ccagaggccg ccgttcactt atactatgaa
      781 agggcgaacg atgcttattt ttcaaaacag tttgatctac gcgccacttc tctcctatct
      841 tgtcacccag gcgctgaaag tggcgatcta ctcttacaaa gacggccgct ttgattacaa
      901 aaggttcttt tcctcgggtg gtatgcccag ctcccacgcg gcgaccgtaa gcgctttggc
      961 cttggcagcc tggcggcagc ttgggccgct ctcgccggtc accgccttgg cccttgtggt
     1021 ggcgctcatt gtcatgtacg acgcccgggg cgtgcggcag gagacgggaa agcacgccaa
     1081 aatattgaat gagctatccg aaaatttatg gggtgcagac acggagagcc gcaataaaaa
     1141 actcaacgag acggtggggc acaccttact gcaggtgctc gtggggggca tgatcggcgc
     1201 tcttatcgca ttccttgtac cgctgaactg atttcgcgac cggaggaggg ggcctcgttt
     1261 taaatggaaa caacccgggc cggcggctgg agcggcaggc gcctttctca caaaggtttt
     1321 ttcatatttt ttgtttttgc cgcccctacc gccttgctgt taaaatgacg tttaagacgt
     1381 gagggactcg ctccccactc ccctccacca caacaaaagc ccgcttaatc gggcttttac
     1441 tttgtcaaaa aaacgagctc taagctccct ggccgctccg cctttatgcc gggcagagcg
     1501 ctctgcgcct tgcgccaggg gccacggccc ccgaaagctg ggattgcttc aggccgcggc
     1561 ggagagccat gcgggccctg taaaagcttt tacagggcac acagagaggg cggcccaaaa
     1621 cagggccgcc ctctctgctt ctttatcctt ttcggctccg tgtccctgcg gggtcaggcc
     1681 ggcggggacg cggtcttctt tttataatcc tcgccccagc tccacatggc gtcgagaatg
     1741 ggcttcaggc tgtaaccggt ctcggtgagg gtatactcca ctcggggcgg cacctcggca
     1801 tagaccttgc gcagcagcag gccgctctcc tccatggcgc gcagctgcgc agtcaagacc
     1861 ttttggctca cctgcccgag ggatcgcttc agctctccaa agcgcttggt ccctcccatc
     1921 aggtcgcgca ggatcagcac tttccatttg tccccgatca gggtcagggt cgtctccacc
     1981 gggcaggccg gcaaggtttt cgcttccaca gacagctccc ctccatccaa tagtatcttt
     2041 ttagtaacta cagcacaaaa ttgtgcttac tttacaaact ctccataccg ccttattata
     2101 aatccagaga caggcgatcg caagccccaa acccaaagaa atacggaggt caaacccatg
     2161 aacgaagtcg tgaaattcct cactgaaaac ccggtgcagt atctggcgac cgtcggccgc
     2221 gacggcaagg ccaaatgccg ccctttcatg tttgccggcg aaaaagacgg caagctgtgg
     2281 ttctgcacca acaataccaa agatgtctac aaggatttgc aggctaatcc ctatacggag
     2341 gtttccgtgt ccagccccgc ctacgcctgg atccgtctaa acggcaaggc tgtgttcgag
     2401 gacaacatgg ctgtcaagga aatgtgcatc cagaacccca tcgtcaaggg ccagtatcag
     2461 accgccgaca atcccatctt tgaggtgttc tatctggacg gcgctcacgc ggtaatcgcg
     2521 gatttctccg gcaacccgcc gcaggaattt gatctgtaaa aaacagccgc aaggcggcta
     2581 tttttcaggc catgcggccc gtcctcgggc cacggccatc gaaatccgcc aggccgtttt
     2641 tcgtctttcc tcttgactgc ctcgacggcg gcaaaagctt ttactcatcc cggccccggc
     2701 cacaccgggg ccttttctgc ggcggccgca gtgagcggcg cggttcctgc atctcccctc
     2761 tctgccgcat cgccgcattt gcccgggctt gccgcctgtg aaaacggttt gaaaacagga
     2821 cataggaggc gtctcatgac caccgtagaa atactcaaaa tgctgcagcg ggacatccac
     2881 tccgccgttt ttgccacggt ggatgagaac ggcctgccgc agacctgcgt catcgacctg
     2941 atgctggccg acgagggcgg gctgtatttt ctcaccgcgc gcggcaaggc gttctaccgc
     3001 cgtctgaccg cggcgggatt tgtcgccctc agcggcatga aaggtgaggg tacgctgtcc
     3061 actgtggcga tttcggtgcg cgggcccgtg cgcaatctgg gatcggcgcg gctggacgag
     3121 atcttcaaag agaaccctta tatggcccgc atctacccaa ctgccgccag caggctggcg
     3181 ctggaggttt ttcagctcta tcgcggcgag ggagaatact tcgacctctc gcagcagccg
     3241 ccgttccgac aagctttttc ctttggcgga cagcaggccg ccccggtcgg atactgcatc
     3301 gacggcgcac gctgtatcgg ctgccaggcc tgcctgctct cctgtcccag cggctgtatc
     3361 cgcgacgccg tgccgcgcag catcgacgcc agccactgcc ttcactgcgg caactgcctg
     3421 cgtgcttgcc cggtgggcgc ggtgaccagg ctgggataaa attttcaggg gggatacgga
     3481 tgaaccggtt ggaacaattg caaaggctga acagcatgct gttagaggag atgcccgagt
     3541 atggggcaca ggcggccgcg ttcccgcccg aggagggcgc gcaacggcgt ttgctgcgca
     3601 gcctgatgaa cctgcgccca cccctgccgc tgcgggcgga ttttttgcag ctgcaggatg
     3661 cgcttttgag cgcggagcgg gttgaaaggg gcgtggtgga agtctcttct ctgccgctca
     3721 ccgccgccga tccccgcctt tgcctgtggc gcggggacat cacccgcctc gcggctgacg
     3781 ccgtcgtcaa cgcggcgaac agcgccctgc tgggctgctt tatcccctgt cacggctgta
     3841 tcgacaatgc gatccactcc gcggcggggc tgcagctgcg cgacgcctgt tttcagctga
     3901 tgcgggcgca ggggcacgag gagcccgtcg gcagcgcgaa gatcacgccc ggctacaacc
     3961 tgccggcccg ctttgttctg cacacggtcg gccccattgt gaatggcccc cttgaggccc
     4021 ggcatcgggc gcagctcgcc tcctgctacc gcgcctgtct ggcgctggcc gcagagcgcg
     4081 ggctggagag cgtcgctttt tgctgcattt ccaccggtga attccacttc cccgccccgg
     4141 aggccgcgca gatcgccgtc ggcgccgtgc aggagtttct gcgggagaaa acttcgatca
     4201 gaaaggtgat tttcaatgtc tttcaagaga gagacgccga tctctaccgc gccctgctcg
     4261 gcccggatcg cgcgcctgag ggctgagctg caagaggccg acgcggtggt ggtgggcgcg
     4321 ggctccggcc tctccgcctc cgccgggctg acctattcgg gggagcggtt cgaaaaaaat
     4381 tttgccgatt ttatcgccga ataccacttt cgtgacatgt actccgccgg cttttacccc
     4441 tatcccaccc cggaggaata ttgggcctac tggagccgtc acatctattg caaccgctac
     4501 gaccgcgcgc ccgggcagcc ctatgccgac ctgctggcgc tgctggaggg caaggattac
     4561 ttcgtcgtca ccaccaacgt ggaccactgc ttccagtccg ccgggttcga caagcgccgg
     4621 ttgttttata cccagggcga ttacggcctg tggcagtgct cgctgccctg ccaccaaaaa
     4681 acctatgaca acgaggcggc cgtgcggcgc atgatcgccg agcagcggcg gatgcgcgtt
     4741 ccctccgagc tggtgcccca ctgcccggtc tgtgggaggc ccatggcgat gaacctgcgc
     4801 tgcgacgaga gctttgtgca ggacgagggc tggtacgccg cccgcgaccg gtacgaggat
     4861 tttctgcgct gtcaccaggg gctgcgggtg ctgtacctgg agcttggggt cggcggcaat
     4921 acgccggtga tcatcaagta ccccttttgg cggctcaccg cccagaaccc caacgccttt
     4981 tacgtctgcg tcaacctggg tgaggcggcc gccccgcggg agctggcagc ccgcagcctc
     5041 tgcatcgacg gggacatcgg tgcggtgctg gcccggctgc ggggctaaaa ccgccgcccc
     5101 tttgccgccc caaaacgcga ttcaagacac gccaataaaa agcgcgcccg ctttcgcgct
     5161 ctgcaaacgg ctccgtatgc tctgagcaac tttccggtcc tgggttcccc cctcggccgc
     5221 ggaaagggct tggagcatat ttcattttga gatggcgcag cgataggaga cgacaaaatt
     5281 gcttcacggc gtgagatcga gccgctggga gaggctgcga ggcggttttg ccaaatatcc
     5341 gccggaaaat caaaaaacca actcctcccg cgcattttgc cttgacgcgt ctgcgcaaag
     5401 ccggacgcgg gtatcccggc tccgcggtca tgaccggggc cggcgaagca gtcctcggtg
     5461 ccctggacag gacaaacggc aggtataccc taccacgctg ctgtttgagc gggcggccat
     5521 gggcgtatgt tattttgcat tgcttgccgc aacgacgggt gtgggctatg atgaacctat
     5581 caaaggaggg acgatccatg ttttgtgaga atttgaaaaa catccgaaga gcaaaaggaa
     5641 tttctcaaga gcttttagcg gagcagcttc acgttgtgcg tcagaccgtg tcaaaatggg
     5701 aaaacgggtt ttctgttcca gatcagacgc ccttatccgg ctggccgagt tttttgaaac
     5761 ctctgtgagc gcgctgttgg gcggcccgat ggaagcgccg gaaaccattg acctggtcgc
     5821 ccaaaagctg gagcagctga accgacagct tgcggataaa aacaggcgaa gcaggcgcgt
     5881 ttggcgcgcc gcggcgggga ttcttgtttt cttgctctct gcggcacact gcagctgttt
     5941 ttaaattttt cggcctgacc caattttctt gaaaatccgg cccgccagcc ccctgcaaat
     6001 aaaaagcaaa tcaaacaaga actcccggcc gcggtgcggc gataaaaagc tgttgcagga
     6061 ccgtttgttt ttccgtttca aattttgggc ggctttgaga gatcaaagcc gcttttctgc
     6121 gctttctcaa aaaagccgcg caaaccggcg ccgccctctc cggtgggcgg caaatgcaaa
     6181 aaaatcgcgg ccaccctctt gacggcaaac cgtatcagcc ttataataca gttaaaccgt
     6241 attattcagc taatacggtt tgagatttaa aacggaaagg ggcggaggcc gtggtttcct
     6301 ttgatcaatt tgtcctgcag gacggcgtcc ccctctatct tcaaatcgtg cgctacctcc
     6361 agcggggcct tgtgtctggg gcgattccgc cgggggcgga gctgccgtcg cggcgggtgc
     6421 tgtcggcgct gctgggcgtc aaccccaaca cggtgcaaaa ggcctaccgc ctgctggagg
     6481 acagcggcgt catcgcctct cacaccggcg caaaaagctt tgtctgcgcg ggtgaggaca
     6541 ccattgcccg catccgcgcc cagctgctgg aaagtgacgt caaaagcgcc gtggaggcgc
     6601 tgcgccaaag cgggctctcc aaatcggagg cgctggcgct ggtggaacgc ttttgggacc
     6661 aaccgtccgg aaaggagcag gtatgaaacg ccatctctcc gcactgatgc tcgccctccg
     6721 cagcaccatc taccgggccg ccgcgctggc gattctggcg ggcgccgccc aggccgcgct
     6781 cttcctgttt gccgcccggc ggctgcaggg cgaggccgcc ccggggctgg aggaggtctt
     6841 tgccgccagc tttgccccgc aggggtgcgc ggtcttttt
//
LOCUS       DBMDYA010000048         6813 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2865_length_6813_cov_1.612783, whole genome shotgun sequence.
ACCESSION   DBMDYA010000048 DBMDYA010000000
VERSION     DBMDYA010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_6813_cov_1.612783"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="ACZPFK_02070"
     CDS             <1..396
                     /locus_tag="ACZPFK_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1405128.1"
                     /translation="IVPDDNSQSVIVFRRMDLAGNEIIAACNFTPVTREDYQFGVPRP
                     GAYKVLLSSDDESFGGSGVKLPAARSRKKPMHGLDNSISVTLPAFSAVYFSVPAEKKA
                     VKKAPAKKAGAKAAGRPAAKKTAKKTVDQ"
     gene            452..1657
                     /locus_tag="ACZPFK_02075"
     CDS             452..1657
                     /locus_tag="ACZPFK_02075"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1405129.1"
                     /translation="MKECIAMLLAGGQGSRLYALTQKLAKPAVPFGGKYRIIDFPLSN
                     CVNSGIDTVGILTQYQPLVLNEYIGNGQPWDLDRLYGGVHVLPPYQKSGGSDWYKGTA
                     NAIYQNINFIDRYDPEYVLILSGDHIYKMDYSRMLAYHKEQKADCTIAVLEVPWAEAS
                     RFGIMTANAEGVITKFEEKPKEPKSNLASMGIYIFSWKKLREYLERDEATPDSDNDFG
                     KNIIPAMLGEGQRMCAYPFEGYWKDVGTIDSLWEANMDLLNPNVPLDVWDESWKIYSR
                     TAAQPPHHITSQAHVENSMITEGCEVEGEVDFSVLFSNVKVHPGAVVRDSIVMPGCVI
                     EEGAQVHYAILAENVVVKKGAIVGARPEHMEDLDKWGVAVVGDGVTVGAGAKVGPKAM
                     ISEDVKEVL"
     gene            1659..1946
                     /locus_tag="ACZPFK_02080"
     CDS             1659..1946
                     /locus_tag="ACZPFK_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1405130.1"
                     /translation="MVNLNAMGIVFPNMHDSVIPELTAHRTMASVPFASRYRLIDFVL
                     SGMTVAGVQNIGVIVKKNYQSLLDHLGSGREWDLARKRGGLTIFPPSGDSG"
     gene            1983..2777
                     /locus_tag="ACZPFK_02085"
     CDS             1983..2777
                     /locus_tag="ACZPFK_02085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF051390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR1405131.1"
                     /translation="MPYIEDAKAKYVILADCDVVCDLDYAALVEKHVASGAEVTIAYT
                     KEKMTPALAKNNISLSLAPSGRVRELLINDPQTGKRNLSMGIVVIGREQLVEIVRTGY
                     AKGLVKFEQDILAKNLETMKVYGYEYTGYRSRIADMSSYFDANMQLLYLENLNRLFPE
                     ERPVYTKVRDEAPVRYALGCKVKNCTVADGCIIEGEIENCVIFRGVHIGKGAVVKNCV
                     LMQNTSVAPGVRMDYVITDKDVSITEGQYLGGTKNFQVFVKKGSTV"
     gene            2849..4273
                     /gene="glgA"
                     /locus_tag="ACZPFK_02090"
     CDS             2849..4273
                     /gene="glgA"
                     /locus_tag="ACZPFK_02090"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR1405132.1"
                     /translation="MNILYCASEALPFAMSGGLGDVAGSLPKALVKAKVNCAVVMPLY
                     GDIKPELREKMTYVTNFTVPVGWRSQYCGVFTAEAAGVTYYLLDNEYYFKRKGLYGFY
                     DDGERFAFFSRAILEMLQHIDFAPDVLHANDWQTALVCVYLNLYYRHIDKFARIKTAF
                     TIHNIQYQGKYGLELLEDVLSIGPENAHLVEYDGCVNYMKGAIECADKVTTVSPTYAY
                     EIMDPWYSHGLDGLLREKAYKTCGILNGIDTVVYDPDTDPLVAYHFDRDTFETGKAQC
                     KDDLRDLFKLERNGEPLIGMVTRLVSHKGLDLVRHVAEYIVLSGMQFVIVGNGDYMYE
                     SFFNELAAKYPGRVGVYIGFNPELAHKVYAGADMFLMPSKSEPCGLAQMVALRYGTIP
                     IVRETGGLKDSITDSGDGQGNGFTFKGYNAHDMLDACLRAKAGYDFPQGWKTLVRRAM
                     DCDFSWKASAKSYIGLYTEMVNLW"
     gene            4515..5225
                     /locus_tag="ACZPFK_02095"
     CDS             4515..5225
                     /locus_tag="ACZPFK_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1405133.1"
                     /translation="MDNRDLHFVDRYLPELSPLLLELDGAVLDKLSLRRLPPDTLLYQ
                     QEDWDESPLYVVVYGVCKLFCNYESLLFPIYCPRGCVIGTLEALSEDFVLRTANMSSF
                     TEVVLLSIPRRLLQHEVYDQSRGLFFWLVRRTILKYHRSGLKYFVRGDVILKLCGYFC
                     DVFAMCRTSTCGEVHSILIRDTQTRLANSTGASVRSVARSVSRMKELGLISTRRGGFL
                     MDDGHYQKMQDFMRQQGF"
     gene            5622..>6813
                     /locus_tag="ACZPFK_02100"
     CDS             5622..>6813
                     /locus_tag="ACZPFK_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405134.1"
                     /translation="MDMVCTFQDNTTKLTDINTESCQYPDLLLGGDGALYACWQSYSQ
                     KHDRIFACVLRDGKPFARLQVSGSGQAFKPVLFELRGRIHVAWSEFSGGRWKLMARAY
                     APEGLGEAVVIGESAGVFFPRAAVDGETAYLAWTQQQQGESRILLCSFDGERAGKPLE
                     VSRGSHCYRPALAVWDGKLCCAYDCFEDGQYFVRALTVEGGAPGRELTLSEGTTWACA
                     PELAVTSSGLTALWYAIAPRADIDYWTAELGMEGGALCRKSAARLARIRDWFASVALA
                     SNGRRDILVHSKSYHAMVARSRQDGDAWSNSVIIYCDAHLCAGVRPRIVVTPDDMVYI
                     IYQYANGNGHRERYADVRLFRASFAEIASRDDGYVDALTNNFTRPIEAPKLLEAVDAE
                     EKRGW"
BASE COUNT         1302 a         2223 c         2102 g         1186 t
ORIGIN      
        1 atcgtgccgg acgacaacag ccagagcgtg atcgtcttcc gccgcatgga ccttgcgggc
       61 aacgagatca tcgccgcctg caacttcacc cccgtcaccc gcgaggacta ccagttcggc
      121 gtgccccgcc ccggcgccta caaggtgctg ctctcgtcgg acgacgagtc gttcgggggc
      181 agcggcgtca agctgccggc ggcccgcagc cgcaaaaagc cgatgcacgg gctggacaat
      241 tccatcagcg tcaccctgcc cgcgttttcc gccgtctact tctcggtgcc ggcggaaaaa
      301 aaggcggtga aaaaggcccc ggcaaaaaag gccggcgcca aggcggcggg gcggcccgca
      361 gccaaaaaga cggcaaaaaa gaccgtggac caataacggc cgccctgccc gcagggatcg
      421 ccgatatgga taaaaaaggg gagaaaccga catgaaagaa tgtatcgcca tgctgcttgc
      481 cggcggccag ggctcccgtc tatacgccct gacccaaaag cttgcaaaac ccgccgtccc
      541 gttcggcggc aagtaccgta tcatcgactt tccgctgtcc aactgtgtca attccggcat
      601 cgacacggtg ggcatcctga cccagtacca gccgctggtg ctcaacgagt acatcggcaa
      661 cggccagccc tgggacctgg accgcctgta cggcggcgta catgtgctgc cgccctacca
      721 gaaatccggc ggcagcgact ggtacaaggg caccgccaac gccatctacc agaacatcaa
      781 tttcattgat cggtatgacc ccgagtacgt gctgatcctg tccggcgacc acatctacaa
      841 gatggactac tcccgcatgc tcgcctatca caaagagcag aaagccgact gcaccatcgc
      901 cgtgctggag gtgccctggg ccgaggccag ccgcttcggc atcatgaccg ccaacgccga
      961 gggcgtcatc accaagttcg aagaaaagcc caaggagccc aagagcaacc tggcctcgat
     1021 gggcatttac atcttcagct ggaaaaagct gcgcgagtat ctggagcggg acgaggccac
     1081 gccggacagc gacaacgact tcggcaaaaa catcatcccg gccatgcttg gcgaggggca
     1141 gcggatgtgc gcctacccgt tcgagggcta ctggaaagac gtgggcacca tcgacagcct
     1201 ctgggaggcc aacatggacc tgctcaaccc caacgtgccg ctggacgttt gggacgagag
     1261 ctggaagatc tattcccgca cggcggcgca gccgccccac cacatcacct cccaggcgca
     1321 tgtggagaac tcgatgatca ccgagggctg cgaggtggag ggcgaggtgg acttcagcgt
     1381 gctcttctcc aacgtcaagg tccaccccgg cgcggtggtg cgcgattcca tcgtcatgcc
     1441 cggctgcgtc atcgaggagg gcgcgcaggt gcactacgcg attttggccg agaacgtggt
     1501 ggtcaaaaag ggcgccattg tgggcgcccg ccccgagcac atggaggacc tggacaagtg
     1561 gggcgtcgcc gtggtcggcg acggcgtcac ggtgggggcc ggggccaagg tgggcccaaa
     1621 agccatgatc tcggaagacg tcaaggaggt gctgtgagat ggtcaacctg aacgcgatgg
     1681 ggatcgtgtt ccccaatatg cacgactcgg tcattcccga actgaccgcc caccgtacca
     1741 tggccagcgt gccctttgcc agccgctatc ggctgatcga ctttgtgctc agcggcatga
     1801 cggtggcggg ggtgcagaac atcggcgtca ttgtaaaaaa gaactaccag agcctgctgg
     1861 accacctggg cagcggccgc gagtgggacc tcgcccgcaa gcgcggcggc ctgaccatct
     1921 tcccgcccag cggcgactcg ggctagggct atcacggccg catcggcgcg ctgaaaggca
     1981 ttttgcccta tattgaggac gcgaaagcca agtatgtgat cctggccgac tgcgacgtgg
     2041 tctgcgatct ggactatgcc gccctggtgg aaaaacatgt cgccagcggc gccgaggtga
     2101 ccatcgccta caccaaagaa aagatgaccc cggcgctggc caaaaacaac atctccctct
     2161 ccctcgcccc ctcgggccgg gtgcgcgagc tgttgatcaa cgacccccag accggcaagc
     2221 gcaacctgtc gatgggcatt gtggtcatcg gccgcgagca gctggtggag atcgtgcgca
     2281 ccggctacgc caaggggctg gtgaaatttg agcaggatat cctggccaaa aacctggaga
     2341 ccatgaaggt ctacggctat gagtacaccg gctaccgcag ccgcatcgcc gatatgtcca
     2401 gctactttga cgccaacatg cagctgctgt acctcgaaaa tttgaaccgc ctcttccccg
     2461 aggagcggcc ggtctacacc aaggtgcggg acgaggcgcc ggtgcgctat gcgctgggct
     2521 gcaaggtcaa aaactgcacg gtggccgacg gctgcatcat cgagggcgag atcgagaact
     2581 gtgtgatctt ccgcggcgtg cacatcggca agggcgccgt ggtcaaaaac tgcgtgctga
     2641 tgcagaacac cagcgtggcg ccgggcgtgc ggatggatta tgtcatcacc gacaaggacg
     2701 tctcgatcac cgagggccag tatttgggcg gcaccaaaaa cttccaggtc ttcgtcaaga
     2761 aagggtccac cgtctgagcc tgccgcaccg tctggggcat gggccgcgcg cccgcgcccc
     2821 gggcatacca tagaggaggg aaacccatat gaatatcctc tattgcgcca gtgaggcgct
     2881 gccctttgcc atgagcggcg gcctgggcga cgtggccggc agcctgccca aggcgctggt
     2941 caaggccaag gtgaactgcg cggtggtgat gccgctgtac ggcgacatca agcccgagct
     3001 gcgcgaaaaa atgacctacg tcaccaattt cacggtgccg gtgggctggc gcagccagta
     3061 ctgcggcgtt ttcaccgctg aggcggccgg ggtcacctac tacctgctgg acaatgaata
     3121 ctacttcaag cgcaagggcc tttacggctt ttacgacgac ggcgagcggt tcgcgttttt
     3181 ctcccgcgcc attctggaga tgctgcagca catcgacttc gcgcccgacg tgctgcacgc
     3241 gaacgactgg cagaccgcgc tggtctgcgt ctacctgaac ctctactacc gccacatcga
     3301 caagttcgcc cgcatcaaga ccgctttcac catccacaac atccagtacc agggcaaata
     3361 cggcctggag ctgctggagg acgtgctctc catcgggccg gagaacgcgc acctggtgga
     3421 gtacgacggc tgcgtcaact atatgaaagg cgccatcgag tgcgccgaca aggtgaccac
     3481 cgtctccccc acctacgcct atgagatcat ggacccctgg tactcccacg ggctggacgg
     3541 gctgctgcgc gaaaaggcct acaagacctg cggcatcctc aacggcatcg acaccgtggt
     3601 ctacgacccg gacaccgacc ccctggtggc ctaccacttc gaccgcgaca cctttgaaac
     3661 gggcaaggcc cagtgcaagg acgacctgcg cgacctcttc aagctggaga ggaacggcga
     3721 gcccctcatc ggcatggtga cccgcctggt cagccacaag gggctggatc tggtgcgcca
     3781 tgtggccgaa tacattgtgc tctcgggcat gcagttcgtg atcgtgggca acggcgacta
     3841 tatgtatgag agctttttca atgagctggc cgccaaatac cccggccgcg tgggcgtcta
     3901 tatcggcttc aatccggagc tggcccacaa ggtctacgcg ggggccgata tgtttttgat
     3961 gccctcgaaa tcagagccct gcggcctggc ccagatggtg gccctgcgct acggcaccat
     4021 ccccatcgtg cgggagaccg gcggcctcaa ggactccatc accgactcgg gcgacgggca
     4081 gggcaacggc ttcactttca agggctacaa cgcccacgac atgctggatg cctgcctgcg
     4141 cgcgaaagcg ggctacgatt tcccccaggg gtggaagacg ttggtgcgcc gcgccatgga
     4201 ctgcgacttc agctggaagg ccagcgccaa atcctatatc ggcctttaca ccgaaatggt
     4261 caacctctgg taaatcctgg atacgcttgc ggcccgcgtc cccgacgcgg gccatttttt
     4321 gcgtccgcgg ggctcttgcc ccgggccgcc cgccttgcgg gcggcctttc tatttgctat
     4381 actgacagtc ataagacaaa atttttacaa ctttcaagcg ccttgccccc tcgcctttcc
     4441 gccgcccggc cgcggcgccg gggccggggc cgggcgcgcc atgacgccgc tttgccgcca
     4501 cagggggaat ggatatggac aaccgcgacc tccattttgt cgaccgctac ctgcccgagc
     4561 tctcccctct tctgctggag ctggacgggg ccgtgctgga caagctctcg ttgcggcggc
     4621 tgccgcccga cacgctgctc taccagcagg aggactggga cgagagcccg ctctacgtgg
     4681 tggtctacgg cgtgtgcaag ctcttttgca actatgagag cctgcttttt cccatctact
     4741 gcccgcgggg ctgcgtcatc ggcactctgg aggccctcag cgaggacttt gtgctgcgca
     4801 cggccaacat gagctctttc accgaggtgg tgctgctctc catcccgcgc aggctgctgc
     4861 agcacgaggt ctacgaccag agccgcgggc tctttttctg gctcgtgcgc cgcaccatcc
     4921 tcaaatacca ccgcagcggg ctgaaatact ttgtgcgcgg agacgttatt ctcaagctct
     4981 gcggctattt ttgcgacgtc ttcgccatgt gccgcacctc cacctgcggc gaggtgcact
     5041 ccatcctcat ccgcgacacc cagacccgcc ttgccaacag caccggcgcc agcgtgcgct
     5101 cggtggcgcg ctcggtcagc cgcatgaaag agctgggcct gatcagcacg cggcggggcg
     5161 gctttttgat ggacgacgga cattaccaga aaatgcagga ctttatgcgg cagcagggct
     5221 tttaacccct gctgccgccc ttttttgccg cgggtgggcc gcggcctttt cccggcgcag
     5281 agccggtctt gccaaagaca aagtgcagcg cgcagggcgc gccgccgggg gagcaacacc
     5341 ccccgtaaag gcggcggacc cggcgcgctg catttcggct ttgcgggctt ttccgccgca
     5401 gggcggcccc gccccgcgct ccccatgggg ttctctcttt ctctgcgctc ccggtctgtc
     5461 aaagctgaat cctccgccct cttgcggccg ttccctgcgc cgtagaaaaa aactgccgaa
     5521 ctttgtcaaa aatgcggccg cctcatcctg ccatttggca ttttcaattt ccgctgattc
     5581 tcttaagatg gaggcaaagt cgatcacagg aggaaaaacg tatggatatg gtgtgcacct
     5641 ttcaggacaa cacgaccaaa ttgacggata tcaatacaga gagctgccaa taccccgacc
     5701 tgcttttggg cggagacggc gcgctgtacg cctgctggca gagctacagc caaaagcacg
     5761 accgcatctt tgcctgtgtg ctgcgggacg gcaagccctt tgcccggctg caggtctcgg
     5821 ggtccgggca ggcgttcaag ccggtgctgt tcgagctgcg cggccgcatc catgtggcct
     5881 ggagcgaatt ttccggcggc cgctggaagc tgatggcccg cgcctatgcg cccgaggggc
     5941 tgggcgaagc ggtggtgatc ggcgaatcag ccggcgtctt tttcccgcgg gcggcggtgg
     6001 acggagagac cgcctacctc gcctggaccc agcagcagca gggggagagc cgcatcctgc
     6061 tctgctcgtt cgacggcgaa agggccggga agccgctgga ggtgtcccgc ggcagccact
     6121 gctaccgccc cgccctggcg gtgtgggacg gcaagctctg ctgcgcctac gactgctttg
     6181 aggacgggca gtacttcgtg cgggcgctca cggtggaggg cggggccccg ggccgggagc
     6241 tgaccctgtc ggagggcacc acctgggcct gcgcgcccga gctggcggtc acctcctcgg
     6301 gcctgacggc gctctggtac gccatcgcgc cccgcgccga catcgattat tggacggccg
     6361 agctggggat ggagggcggc gccctgtgcc gcaaatccgc cgcccgtctg gcccgcatcc
     6421 gcgactggtt cgcctcggtg gccctcgcct ccaacggcag gcgcgacatc ctggtgcact
     6481 ccaagagcta ccacgccatg gtggcccgca gccggcagga cggcgacgcc tggtccaact
     6541 cggtcatcat ctactgcgac gcccatctct gcgccggggt gcgcccccgc atcgtggtca
     6601 cccccgacga catggtctac atcatctacc agtacgccaa cggcaacggc caccgcgagc
     6661 gctacgccga cgtgcggctg ttccgggcca gctttgctga gatcgcctcg cgggacgacg
     6721 gctatgtgga cgcgctgacc aacaatttca cccggcccat cgaggcccca aagctgctgg
     6781 aggccgtgga cgcggaggaa aaacgcggct ggc
//
LOCUS       DBMDYA010000049         6805 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2872_length_6805_cov_1.512828, whole genome shotgun sequence.
ACCESSION   DBMDYA010000049 DBMDYA010000000
VERSION     DBMDYA010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2872_length_6805_cov_1.512828"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            61..1704
                     /locus_tag="ACZPFK_02105"
     CDS             61..1704
                     /locus_tag="ACZPFK_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 family acyl-acid amido synthetase"
                     /protein_id="HIR1405135.1"
                     /translation="MTFEEKLKTCSAGEVWQEYCGFLDLSMEEYMGIQRRLLMEQIDL
                     MGKCALGKRFFGDDPPKTVEEFRRRVPLTSFGDYADVLLTRREEMLPAKPVVWLRTTW
                     EGGDFPAKYAPYTESMLDTYKHNMLGAVLLATSSAKGKFHVRPGSRILYSLAPMPYAT
                     GLFPDLIEPELRVRFLPSVREARKLSFGQQNRRGYKLALQQGMNMFFGMSSVLYGTTR
                     NLEQMNRSSQEKSGGLRSLLGISPRMLWRLLSGRYRSRRDGKPLMPRDLFRLDGFVCI
                     GTDSSRYKDELEAAWGVRPMELAGGTEPTCLGTETWSKNGLVFFPDACFYEFIPEQEM
                     LRNLADPSYKPHTYLIDELVAGQNYELVITVLRGGAFLRYRVGDVYRCLRLRNPQDGL
                     ELPQFEYVDRIPTVIDIAGFTRITEREIRKVIEMSCLPVADWFALKEYDDNGHSFLRL
                     YVELQAGARQSPALSREIIREHLSVYFRYYDGDYKDLKRLLGIDPLEVTMLPTGTIAR
                     YEAASGQKLPRVGAPREAVLDLLHLPPASASGEGDGRCR"
     gene            1695..2090
                     /locus_tag="ACZPFK_02110"
     CDS             1695..2090
                     /locus_tag="ACZPFK_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405136.1"
                     /translation="MSLNQRLFIIVPLVSALCNIFLLLTVLSAKKNKQIYAFMELLCA
                     FTAWCTGSLFMRLTVYPGPEFWYEVSIMGIFLTPFFVYNFMVLLHRVQGRLCAGHAVC
                     QLGGGDGPQPVPRLHHQPAGGGGGRRAPV"
     gene            1981..4236
                     /locus_tag="ACZPFK_02115"
     CDS             1981..4236
                     /locus_tag="ACZPFK_02115"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR1405137.1"
                     /translation="MRGTLFASWVVVTALNLFHVFITSPRVVEEAGERRFEYGVSPAV
                     IVPILLVVVTLWEAWRLAYKSVRDDRVPLSQFTPMIAGVAVMFACTVAAVLPQMVSLP
                     VDTFSCGINAICLYYALYKKRMIQLRGIASNGPAYLVAALLTTLVLIAGYQPVEAFYG
                     RYFADYQEYKVIVFAVGFSLVTMAVYSVVRRLMSNLFVKGQENQEAQLKQFSSAINKT
                     LKLDEILELYRDFLQRNLPGKTARVFLYQPSAGGYQVADCTNSTLAKGDFIAADNPLV
                     PWLKSYNHGVSYAEFQRTRIYRSLWEEEKRRFAEMRISFILPVVCDDELTAITTFSDE
                     AGPERARELSFREVNFLESMAAVLSMALKNASLYAAIENKARRDPLTNLFNRGYFEEC
                     IRRDFELCRHDSLSLLMISFDDFHLYNELYGTSEGDRILRRFGEQLAALVGARGTVAR
                     YGGKDFAVCLPFCTASVADTLARQARDWLRYEVSSASEKTKKFLTFSAGICAYPVSAA
                     TVEELFTYANMAVYTAKSNGKNRIVLYDPGQNREEPDSNFQTKRALADNCAATIYALT
                     AAIDAKDHYTFSHSNNVAEYASILAAAIPLDAEHVEIIRQAGLLHDIGKIGIPEAILS
                     KTARLTKEEYEVMQQHVEGSIAMIRYLPSLDYVIPSAIGHHERWDGKGYPRGIAGETI
                     PIGARCLCLADSFDAMVSKRSYKEAMSVEEALQEIRRNLGTQFDPDLGLLFIRLVEEG
                     RIQPHLSAAEE"
     gene            4728..5435
                     /locus_tag="ACZPFK_02120"
     CDS             4728..5435
                     /locus_tag="ACZPFK_02120"
                     /inference="COORDINATES: protein motif:HMM:NF018766.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HIR1405138.1"
                     /translation="MAKKRKLAATGACAAAYAAACLALSRLTGDPLPAGLAWNLLLAL
                     LPGPLLSAALARRGVLQVGLAALWLLFLPNTFYLLTDLIHVPPQMEWLGAGERGAAVL
                     HSEKASQWALVLLIGIGAFWGVWLGLESLSRAFDFARSRAGRLPAWAAVGALALLCGT
                     GIYIGRFLRLNSWDALHPLRLLGRVAASLDGFACKIILLLAASILLLFCFYRARCPGV
                     EAGAGEEDGGGTGENRL"
     gene            complement(5747..6151)
                     /locus_tag="ACZPFK_02125"
     CDS             complement(5747..6151)
                     /locus_tag="ACZPFK_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405139.1"
                     /translation="MLSDGLAVLVSFAALALIPTAIVLGILAASRSARKKKKQRYTGV
                     TTGTVVNVERKGLDYPWVICASYRVDNISYKIKETAKLKTSSLKVGSVPIGQRKTFVL
                     GPLKAGDPILVHYDEKNPQKAILYENDGVVTG"
     gene            complement(6375..>6805)
                     /locus_tag="ACZPFK_02130"
     CDS             complement(6375..>6805)
                     /locus_tag="ACZPFK_02130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1405140.1"
                     /translation="NVGAIFRSAAALGMDAVLLTPTCCDPLCRRAARVSMGAVFQVPW
                     ARIGAEAGDWPQPGVTRLQGLGFKTAAMALSDTALSIEDPRLAAEPKLAVVLGTEGDG
                     LAPRTIAACDYTVRIPMSHGVDSLNVAAASAVAFWQLRAR"
BASE COUNT         1163 a         2110 c         2293 g         1239 t
ORIGIN      
        1 cccggccgca gaatgagaac ggcccccggg gtgtggggga tggaaaagag gaggccgcgg
       61 atgacctttg aagaaaaatt gaaaacctgt tccgccggcg aggtctggca ggagtactgc
      121 gggttcttgg acctctcgat ggaggagtat atgggcattc agcgccggct gctgatggag
      181 cagatcgacc tgatgggcaa gtgcgccctg ggaaagcgct ttttcgggga cgacccgccc
      241 aagacggtgg aggagttccg ccgcagggta ccgctgacca gctttggcga ttacgccgac
      301 gtgctgctga ccaggcggga ggagatgctg cccgcaaagc cggtggtctg gctgcgcacc
      361 acctgggagg gcggggattt tccggcaaaa tacgccccct acaccgagag catgctggac
      421 acctacaagc acaatatgct gggggcggtg ctgctggcca ccagcagcgc caagggcaaa
      481 ttccatgtga ggccgggctc ccgcattttg tacagcctcg cgcccatgcc ctatgccacc
      541 ggcctttttc ccgatttgat cgagccggag ctgcgggtgc gctttctgcc ctcggtgcgg
      601 gaggcccgca agctctcgtt tggccagcag aaccggcgcg gctacaagct ggcgctgcag
      661 cagggcatga atatgttctt tggcatgagc agcgtgctgt acggcaccac ccgcaacctg
      721 gagcagatga accgctcctc gcaggagaaa agcggcggcc tgcgctcgct gctgggcatc
      781 agcccgcgca tgctctggcg gctgctctcg ggccgctacc gctcgcggcg ggacggcaag
      841 cccctgatgc ccagggacct gttccggctg gacggctttg tctgcatcgg caccgactcc
      901 agccgttaca aggacgagct ggaggccgcc tggggcgtgc ggccgatgga gctggcgggt
      961 ggcaccgagc cgacctgcct tggcaccgag acctggagca agaacggcct ggtctttttt
     1021 cccgacgcct gtttttacga gttcatcccc gagcaggaga tgctgcggaa tctggccgac
     1081 ccgtcttaca agccgcacac ctatctgatc gacgagctgg tggcggggca gaattacgag
     1141 ctggtcatca cggtgctgcg gggcggcgcg tttctgcgct accgggtggg ggacgtctac
     1201 cgctgcctgc ggctgcgcaa cccgcaggac gggctggagc tgccgcagtt cgagtatgtg
     1261 gaccgcatcc ccaccgtcat cgacatcgca ggcttcaccc gcatcaccga gcgggagatc
     1321 cgcaaggtga tcgagatgtc ctgcctgccg gtggcggact ggtttgccct gaaagaatac
     1381 gacgacaacg ggcactcttt tctgcggctg tatgtggagc tgcaggcggg ggcgcggcag
     1441 agcccggccc tgagccggga gatcatccgc gagcacctgt cggtctattt ccgctattac
     1501 gacggcgatt acaaggacct gaagcggctg ctgggcatcg acccgctgga ggtgacgatg
     1561 ctgcccaccg gcaccatcgc gcgctatgag gccgccagcg ggcaaaagct gccccgggtg
     1621 ggcgcgcccc gcgaggcggt gctggacctg ctgcatctgc cgccggcgtc cgcatcggga
     1681 gagggggatg gaagatgtcg ctgaaccagc gtctgtttat catcgtgccc ctggtctccg
     1741 cgctgtgcaa tatctttttg ctgctgaccg tgctcagcgc caaaaagaac aagcagattt
     1801 atgcctttat ggagctgctg tgcgccttta ccgcctggtg caccggctcg ctcttcatgc
     1861 gcctgaccgt ctatccgggg ccggagttct ggtacgaagt ctcgatcatg ggcatctttt
     1921 tgaccccgtt tttcgtctat aacttcatgg tactgcttca ccgagtccaa gggcggcttt
     1981 gtgcggggca cgctgtttgc cagctgggtg gtggtgacgg ccctcaacct gttccacgtc
     2041 ttcatcacca gcccgcgggt ggtggaggag gccggcgagc gccggtttga gtacggggtc
     2101 tcgcccgcgg tcatcgtgcc catcctgttg gtggtcgtca cgctgtggga ggcctggcgg
     2161 ctggcctaca agagcgtgcg ggacgaccgg gtgcccctgt cccagttcac ccccatgatc
     2221 gccggtgtgg cggtcatgtt cgcctgcacg gtggccgccg tgctgccgca gatggtctcg
     2281 ctgccggtgg acactttctc ctgcgggatc aacgccatct gcctctacta cgccctttat
     2341 aaaaagcgca tgatccagct gcggggcatc gcctccaacg ggcccgccta tctggtggcg
     2401 gccctgctga ccaccctggt gctcatcgcc gggtatcagc cggtggaggc gttctacggg
     2461 cggtattttg cggattatca ggagtacaag gtcatcgtct tcgcggtggg cttttcgctg
     2521 gtcaccatgg cggtctacag cgtggtgcgg cggctgatga gcaacctgtt cgtcaagggg
     2581 caggagaacc aggaggccca gctcaagcag ttttccagcg ccatcaacaa gaccctgaag
     2641 ctggacgaga tcttggagct ctatcgcgac tttttgcagc gcaacctgcc gggcaagacg
     2701 gcgcgggtct ttctctatca gccgtcggcg ggcggttatc aggtggccga ctgcaccaat
     2761 tccaccctgg caaaggggga tttcatcgcg gcggacaatc cgctggtgcc ctggctgaag
     2821 agctacaacc acggcgtgag ctacgccgag tttcagcgca cccgcatcta tcgctcgctg
     2881 tgggaggagg agaagcgccg cttcgccgag atgcgcatca gctttatcct gccggtggtc
     2941 tgcgatgacg agctgaccgc catcacgacc ttttcggatg aggcggggcc cgagcgcgcg
     3001 cgggagctga gcttccgtga ggtcaacttt ctcgagtcga tggcggccgt gctctcgatg
     3061 gcgctgaaaa acgcctcgct ctatgcggcc atcgagaaca aggcccggcg cgacccgctc
     3121 accaacctgt tcaaccgcgg ctattttgag gagtgcatcc ggcgtgattt tgagctctgc
     3181 cgccacgaca gcctgtcgct gctgatgatc agcttcgacg attttcacct gtataacgag
     3241 ctctacggca cctctgaggg cgaccgcatc ctgcgccggt tcggcgagca gctggccgcc
     3301 ttggtgggcg cgcggggcac cgtggcccgc tacggcggca aggattttgc cgtctgcctg
     3361 ccgttttgca ccgcctcggt ggccgacacc ctggcccggc aggcgcggga ttggctgcgc
     3421 tatgaggtga gcagcgcgtc ggaaaagacg aaaaagttcc tcacgttcag cgccggcatc
     3481 tgcgcctatc cggtgtcggc ggccacggtg gaggagctgt tcacctacgc caacatggcg
     3541 gtctataccg ccaagtccaa cggcaaaaac cgcatcgtgc tctatgatcc cgggcagaac
     3601 cgggaggagc cggacagcaa ttttcagaca aagcgggcgc tggccgacaa ctgcgcggcc
     3661 accatctacg cgctgaccgc ggccatcgac gcaaaggacc actacacgtt cagccactcc
     3721 aacaacgtgg ccgagtacgc ctccatcctg gcggcggcca tcccgctgga cgccgagcac
     3781 gtggagatca tccggcaggc ggggctgctg cacgacatcg gcaaaatagg catccctgag
     3841 gctatcctgt ccaagaccgc ccgactgacc aaggaggaat acgaggtcat gcagcagcat
     3901 gtggagggct ccatcgccat gatccgctat ctgccctccc tcgactatgt catcccctcg
     3961 gccatcgggc atcacgagcg ctgggacggc aagggatatc cgcgcggcat cgccggggag
     4021 acgatcccca tcggcgcgcg ctgcctgtgc ctggccgact cgtttgacgc catggtgtcg
     4081 aagcgcagct acaaagaagc catgagcgtg gaggaagcgc tgcaggagat ccgccgcaac
     4141 ctgggcaccc agttcgaccc cgacctgggc ctgctcttca tccgcctggt ggaggagggg
     4201 cgcatccagc cgcacctgtc cgcagccgag gagtagagga acaagcgcgc gccccggctg
     4261 cccgtgtggc tgggcgcggg gcgcaggagg aagaatttcc gccgcgggtt tgaaagagcg
     4321 gcccggcaaa aagcgcgggg aggacgcgaa cgcttggctt tggctgagag agcaaaactg
     4381 cgcggcgccg acggcgtgcg gaaaagagcc ggcgacaagg gcgcggggcg cggatcaccg
     4441 cgagcccggc ggatccgcaa gcggcgcgca ggcggtggac gggcttttcc gtcaaaagca
     4501 ggcggccgct tttcccggtc aaaactgcgc gaatggacgg cgctcgctac cgctttgcca
     4561 caccgcgcgt aaaatgaaaa agcagccggc ccacttttga aaaaagcggg ccggcgttgt
     4621 ttttttgcgc ccggcgtgat aaacttaccc aaaagccggt ggagagacgg gcgctgcgcc
     4681 gggcgccgac gcgcccgcat tgtcggacgg ggaagagggg gaggggcatg gcgaaaaaac
     4741 gaaagctcgc ggcgacgggc gcctgcgcgg cggcctatgc ggcggcatgt ctggcgctgt
     4801 cacggctgac cggcgacccg ctgcccgcgg gcctggcgtg gaacctcctg ctggcgctgc
     4861 tgcccggccc gctgctgtcg gcggccctgg cgcggcgcgg tgtgctgcaa gtcggcctcg
     4921 cggccctgtg gctgctcttt ttgcccaaca ccttttacct gctcaccgac ctcatccacg
     4981 tgccgccgca gatggagtgg ctcggcgccg gtgagagggg cgcggcggtg ctccacagcg
     5041 aaaaggcttc ccagtgggcg ctggtgctgc tgatcggcat cggggccttt tggggggtat
     5101 ggctggggct ggagtccttg tcccgcgctt tcgatttcgc gcgttcccgg gcagggcgct
     5161 tgcccgcctg ggctgcagtg ggcgcgctgg ccctgctctg cggcacaggg atctatatcg
     5221 gccgctttct gcggctcaac agctgggacg cgctgcatcc cctgcggctg ctggggcggg
     5281 tcgcagccag cctggacggc tttgcctgca agatcattct gttgctggcg gcgtccattc
     5341 tgctgctgtt ctgcttttat cgcgcccgct gtccgggggt ggaagccggg gcgggggaag
     5401 aggacggcgg cgggacgggt gagaaccggc tttgagagca agcctctccc gcctccccca
     5461 atacctgcgc atccaaaatg cagcggcggg gagcagttgg ccttgcccgg caggacaaaa
     5521 ctgcgcggca gccgctggcc gcggaaaaga gccgacagga ggaacgccgc aagcctggga
     5581 gcgcaagcgg cgcgggatcg gcagacggga gttctccgtc ggaagcggac atcaaccatt
     5641 ttcaaccaaa accgcgcgaa agacgcgccc tttccggcga tctgccggca ggggcgcgtc
     5701 ttttggggtg aggcctgagg agaagcgggg tgccggagcg gcgctttcaa ccggtgacga
     5761 caccgtcgtt ttcgtataaa atcgcctttt gcggattttt ttcgtcgtag tgaacgagga
     5821 tggggtcgcc cgctttgagg ggcccgagga cgaaagtctt tctctggccg atggggacgc
     5881 tcccgacttt gagggagctg gtttttaatt tcgcggtctc tttaatcttg taagagatgt
     5941 tgtcgacacg gtaactcgca cagatgaccc aggggtaatc caggcctttt ctttcgacgt
     6001 ttaccaccgt gccggtcgtc acgccggtgt accgctgctt ctttttcttt ctggccgatc
     6061 ggctggctgc caatatcccc aggacgattg cggtggggat aagggccagg gcggcaaaac
     6121 tcaccagcac tgcaaggccg tcgctcaaca taaattattc cctcctgttc ttgactgggc
     6181 cgcgtttttc cgggcaggcg caggcgggag cgcaccgcgc ctcgccgcgc tcgcgcgctg
     6241 tggtgggaaa ggacgaaaaa aacagcgctg ttgcccatcc gttatgcgga cgcgtgaaaa
     6301 gcggttgggc gtctcctaag cttagaggtt taatttagac acaggataaa aaacaggccg
     6361 ccgtgtgggc gcgctcaccg ggcccgcagc tgccagaacg ccaccgcgct ggcggccgcc
     6421 acgttcagcg aatccacgcc gtgggacatg gggatgcgca ccgtgtagtc gcaggccgcg
     6481 atggtgcggg gggccaggcc gtcgccctcg gtgcccagca cgacggcgag cttcggttcc
     6541 gccgcgagcc gtgggtcctc gatgctgagg gcggtatcgc tcaaagccat ggcggcggtc
     6601 ttgaagccca gcccctgcag ccgcgtgacg cccggctggg gccaatcgcc cgcctcggcg
     6661 ccgatgcggg cccagggcac ctgaaagacg gcgcccatgc tcacccgcgc cgcgcggcgg
     6721 cagagcgggt cgcagcaggt gggggtcagc agcaccgcgt ccatgcccag cgcggcggcg
     6781 gagcgaaaga tggcccccac gttgg
//
LOCUS       DBMDYA010000050         6617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2965_length_6617_cov_1.601136, whole genome shotgun sequence.
ACCESSION   DBMDYA010000050 DBMDYA010000000
VERSION     DBMDYA010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2965_length_6617_cov_1.601136"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(967..1434)
                     /locus_tag="ACZPFK_02135"
     CDS             complement(967..1434)
                     /locus_tag="ACZPFK_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015060.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR1405141.1"
                     /translation="MKWKKYLPDLLFVLLSLAVGGLSAFAVMKGMPAYLQLRKPPLTP
                     PPIVFPLVWGALYLLMGLGAGRVWRAGPPRRAAVAAYAVQLVWNALWTVWFFALQAHL
                     FAFWWLAALLGLIALMLGLFARADRPAAWMQLPYLLWTVFAAYLDLGVWLLNR"
     gene            1503..1790
                     /locus_tag="ACZPFK_02140"
     CDS             1503..1790
                     /locus_tag="ACZPFK_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405142.1"
                     /translation="MNSQQRAPDRGDETQGPQLRRRAATSAEQDRETDRKNGITACRG
                     CVYYRSACGVPEAYMMCHYAWDTGRCRGLRPADCYRRAGTPYRPRRGRRGP"
     gene            1787..2260
                     /locus_tag="ACZPFK_02145"
     CDS             1787..2260
                     /locus_tag="ACZPFK_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405143.1"
                     /translation="MSRPAKLPPGVIRACAGLLESEEALSLACRRAIQAAENAVGSDY
                     LPEAEQQRRQLIQAIKLSLVNRKEWPYEALARTFDLSMSYNAFGREKKKFCYVMATGA
                     GLVRLHRRPRRRTVELCPLPEPGGEGAGPWGGQARQGEAEDAQNDTKSRGGKRNG"
     gene            2253..4055
                     /locus_tag="ACZPFK_02150"
     CDS             2253..4055
                     /locus_tag="ACZPFK_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405144.1"
                     /translation="MDEFWKKRNKPSAAQLTGALGESVKEYLKKTELPGEKAAVTLNS
                     FYPADAGTLVRTLEEIGGPEKRWSWQGGQAEDKTGYGGPAAQRQGQSGKGGWGQSGEP
                     GFEQKVALVQQALQRGREAHRRQEGNHYTAGNTAGRSSGSFGGGNLNGESFPGAGTSG
                     VPGGPAVGRSRPAFPTAVQGGAGPSLVGGDSLLDGRGGSLVSGENFLGGSPAGSTIGK
                     GKGGRGGLFPDGERTSGAFSGGRASGGGRSGLFPDEERTSGAFSGSRAAGSFSGGASS
                     GTSGGAGIGRNDGGPAAAQEERQQYEVRSPADFTAAVWQQLNELPQDFADTEEPWYDK
                     FERGNVIGGVGSLLGTGLRAPQTLLLNATDALTAGLTGQQAQFQPDLTPDKYLRNMEA
                     YGGQPGFASFLDELEASGLPGAVGANLLRLGYDWGTNPASLLDAAAWQKTQGLQQPGS
                     GNYIDDLQNGGWWERGEDSFWTQQNEKPENFSFDTGETSQEAEKIPWNTWENYEKVVQ
                     DGQEYALVGGRPYSHHAVDRMQPSGRRYAAAGSIFQAGGVDGRSVAPQFIEEILQSVR
                     PLYQPRTGNFEYKIGTIKIITNSKGSVVTIMIYQ"
     gene            4069..4335
                     /locus_tag="ACZPFK_02155"
     CDS             4069..4335
                     /locus_tag="ACZPFK_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405145.1"
                     /translation="MEYLENLVKLCEDFKQKGFETKELQERLETALLPDNLSAGYRKA
                     LHNFHNDLEEILYFYPPEEQAARTMEVVERMLAATRLEQAGIYT"
     gene            4362..5534
                     /locus_tag="ACZPFK_02160"
     CDS             4362..5534
                     /locus_tag="ACZPFK_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HIR1405146.1"
                     /translation="MQAPLSGDTLEERNQIGQALLRLSQQGADENAKASEGQGGGTEY
                     EPSSHKWDVAREWGVDPDELMWDFRKNGYVLEEDFIKSLKGDGEVAAIAGADPYYGPV
                     RSAEPREEEKTQPEEENSGREKTKGKDQLRRLNYSILEENPKVNDLSKEELQDILLDF
                     QINIQKFNPYTDPPKKYVKQNGKGDWIPAEKSEVRAAMFPDPYDPVQKFQFMDVRVDP
                     EEGSGLTAEQIDDYLSQNTNAKLFRGHGQDFIDAEKASGINAAFLVASAICEQGELSK
                     GTSSAAPGFFNVYGIGATDGEAQLGGGETARKYGWTSLSYAIKGGAYILRKDWVDAGQ
                     ATAYDMRWNPSHPTQHWYSTAIIAASERAKYMEGIYSYCLDGDTTGLVFTYDDRYL"
     gene            5549..>6617
                     /locus_tag="ACZPFK_02165"
     CDS             5549..>6617
                     /locus_tag="ACZPFK_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405147.1"
                     /translation="MKMRDRIATIVLACVLFCTALAGCGAATGLSESASVPISDVSPL
                     SSGTVSPTPTPTLRIDPSCPPEEINFTPEEALLYFAWLYWGKEEGRDFAATEREDLYI
                     GGYPGEGIDPREGSYQATIADDIMYWEGEFLYTVYFSVVYPARPSGEALGMFFINVNN
                     LKMWYDFLPDEGKINRAVERSLLVEFLQHIGKEDLTEEVLAQYEEDENGTLWPYGRPT
                     GTPEPTAPPRKTPAPTSTPDPAWGKRVGVDELAALESQEKAVRYSKEECLEMAARTLA
                     AGGAVETGLPGSLATFPCVEYAGAPGCRLILVPGMFWSPKNEPWAYGYEFMIYVEDAS
                     QLREGAVPPPEGPGKEYQTEEN"
BASE COUNT         1472 a         1788 c         2286 g         1071 t
ORIGIN      
        1 gaggaccttg ggggaattgt cccggacggg gaaaaccgca cagagccggg cccgggcctt
       61 gaggcacggg ggtgatcccc acggggactg ccccggacag ggagagcggc gcggacgggg
      121 gcctggggat gagaacctcg agggagttgt cccggacagg gaaaaccgcc cagagccgga
      181 cccgggcctt ggggcacggg gatgaccccc gcggggactg ccccggacag agagagcggc
      241 gcagacgggg gcgcggggat gagaaccttg ggggaattgt cccggacggg gaaaaccgcc
      301 cggaccccag ggcagggccg cacggataag cagccgcaag aaaaacctcc gggttcgctc
      361 ggcgcaccgg ccgcccctcc ggttcccgga ttctttcaca cggcgagaca cagcagccct
      421 cttttccttt cggggcggat cgcgccgacg ccctttcccg ccctccgcgt tttttgcagg
      481 atggggagga attcccactc tcgcggggct ggttcgcgca tcgcctttcc ggctcccgcg
      541 cggcggcagc cgtccccctt gcccggggaa gcaaaaagtg cgcgatttgg ggcttgcacg
      601 cctttcacga agacgcgtgc gttggctccc ctcgtgggga ggccgaaaag cgcggcttgc
      661 ggcccacgcc gatttctgcg gcggcgccct caccggcttc ccttgcacgc gaaccggcgt
      721 gcgctggccg tgtaagggag tttcccacgg cgagacagag gggcctcctt tccctttcgg
      781 gccggatcgc gccgccgtct ttacccactc ccgcgttttt tcgcgtggcg gggtgaaagc
      841 tccgtttcct ctggggccgg accggcgcgc cgcctcctcg ctccccgcgc ggcgggagcc
      901 gtcctccttg ccggggaact aaaaagtacg cggcctgcgc cgatcccagc ggcccgcggc
      961 ggccggtcaa cggttcagca gccagacgcc caggtccagg taagcggcaa agacggtcca
     1021 cagcaggtag ggcagctgca tccaggctgc ggggcggtcc gcccgggcga agaggcccag
     1081 cattagcgcg atgaggccca gcagcgccgc cagccaccaa aaagcgaaca aatgcgcctg
     1141 cagggcaaag aaccagaccg tccacagggc gttccacacc agctgcaccg catacgcagc
     1201 cacggcggcg cggcgcggcg ggcccgcccg ccacacccgg cccgcgccca ggcccatcag
     1261 caggtacagc gccccccaca ccagggggaa cacgatgggc ggcggggtca gcggcggctt
     1321 gcgcagctgc agataggcgg gcatgccttt catcaccgca aaagcggaca gaccgcccac
     1381 cgccagtgag agcagcacaa agagcagatc aggcagatat tttttccatt tcacacggca
     1441 tcaccagtgg ctagtatgcg ccgccgcggt gccgatatga atcacgagcc ggaggtagag
     1501 agatgaacag ccaacagaga gcgcccgaca ggggcgatga aacacagggg ccgcagctgc
     1561 ggcggcgggc cgcgaccagc gccgaacagg accgggagac cgaccgcaaa aacggcatca
     1621 cggcctgccg gggatgcgtg tactaccgct cggcctgcgg ggtgccggag gcctatatga
     1681 tgtgccatta cgcctgggac accgggcggt gccggggcct gcgccccgcg gattgctacc
     1741 gccgcgcggg cacgccgtat cgtccccggc gcggcaggag ggggccgtga gcaggcccgc
     1801 gaagctgccc cccggcgtga tacgcgcctg cgccggcctg ctggagtcgg aggaggcgct
     1861 ctccctcgcc tgccgcaggg ccatccaggc ggccgaaaac gcggtgggca gcgactacct
     1921 gcccgaggcg gagcagcagc ggcggcagct gatccaggcc atcaagctgt cgctggtcaa
     1981 ccgcaaagaa tggccctatg aagcgctggc gcgcaccttt gacctgtcga tgagctacaa
     2041 cgcctttggg cgcgaaaaga agaagttttg ttatgtgatg gccaccgggg cgggcctggt
     2101 gaggctgcac cgcaggccgc ggcgcaggac ggtggagctc tgccccttgc cggagccggg
     2161 cggggagggc gcggggccgt ggggcgggca ggcccggcag ggagaagcgg aggacgcaca
     2221 aaacgacaca aaatcaagag gaggcaaaag gaatggatga attttggaag aagcggaaca
     2281 agccgagcgc ggcgcagctg acgggcgcgc tgggggaatc ggtgaaagag tacttaaaaa
     2341 agacggagct gcctggggag aaagcggcgg tgacgctgaa cagcttttac cccgcggacg
     2401 cggggacgct ggtgcgcacg ctggaggaga tcggcggccc ggaaaagcgc tggagctggc
     2461 agggcggaca ggccgaggat aaaacggggt acggcggccc ggccgcacag cggcagggcc
     2521 aaagcgggaa agggggctgg ggccaaagcg gagagccggg ctttgagcag aaggtggcgc
     2581 tggtgcagca ggcgctacag cggggccggg aggcgcaccg caggcaagag gggaaccact
     2641 acacggcggg gaacacggcg ggccgcagca gcggcagctt tggcggcggc aacctgaacg
     2701 gagagagctt tcccggcgca gggacgagcg gcgtccccgg cggacccgcg gtcggccgca
     2761 gccgcccggc ttttccgacg gcggtgcagg gcggcgcggg gccgtctctg gtgggcggcg
     2821 acagcctgct ggatggacgc ggcggcagcc ttgtgagcgg agaaaatttt ttgggcggca
     2881 gcccagccgg gagcacaatc ggcaagggga aaggcggccg cggcgggctt ttcccggatg
     2941 gagagcgcac ctcaggcgcc ttttccggcg gccgggcttc cggcggcggc cgcagcgggc
     3001 ttttcccgga tgaagagcgc acctcaggcg ctttttccgg cagccgcgct gcgggcagtt
     3061 tctccggcgg ggcgtcgtcg ggaacctccg gcggcgcggg cataggccgc aacgatggcg
     3121 gcccggcggc agcgcaggag gagcgccagc agtacgaggt acgctcaccg gcggatttca
     3181 cagcggcggt gtggcagcaa ttgaatgaat tgccgcagga ctttgcagac acggaagaac
     3241 cgtggtatga taagtttgaa cgcggtaatg tgatcggggg cgtgggcagc ctgctgggca
     3301 ccgggctgcg ggcgccgcag acgctgctgc tgaacgccac ggacgcgctg acggcgggcc
     3361 tgaccgggca acaggcgcag ttccagccgg atctgacgcc ggacaaatac ctgcgcaaca
     3421 tggaggcgta cggcggacag ccgggattcg ccagcttttt ggacgagctg gaggccagcg
     3481 gcctgccggg tgcggtgggg gcaaacctgc tgcggctggg ctatgactgg ggcacaaacc
     3541 cggcaagcct gctggatgcg gcggcctggc aaaaaacaca gggcctgcag cagccgggca
     3601 gcggaaacta catagatgat ctgcaaaacg gcggctggtg ggagcgggga gaggacagct
     3661 tctggaccca acaaaatgaa aagccagaga acttctcttt cgatacggga gaaacttctc
     3721 aagaagcaga gaaaatccct tggaacactt gggaaaatta tgaaaaagtt gtgcaagacg
     3781 gacaggaata tgcactcgta ggaggacggc cctattctca tcatgctgtc gatcggatgc
     3841 agccgagtgg aagacggtat gcggctgcgg gttccatttt ccaggcggga ggagtagatg
     3901 gcaggagtgt agcaccgcaa tttattgaag aaattcttca gagtgtccgg cctctttatc
     3961 aaccacggac aggaaatttt gaatacaaga tcggtacgat caaaattatt acaaattcca
     4021 aagggtctgt tgtcaccatt atgatttatc agtaagagga gaagtgaaat ggaatattta
     4081 gagaatctgg taaagctctg tgaggatttc aagcagaaag ggtttgaaac gaaagagctt
     4141 caagagcggc ttgagacagc cctgttgccg gacaatcttt ctgccggata ccgaaaagct
     4201 ttgcacaatt ttcacaatga tctggaggaa atcctttatt tttatccgcc cgaggagcag
     4261 gcggcccgga cgatggaggt ggtggaaaga atgcttgctg ccactcgact ggagcaggct
     4321 gggatataca cataaaggcc ggaaacgagc ggcaaaaaag attgcaagcg ccgctttcgg
     4381 gtgatacgct tgaggagaga aaccagatcg gacaggccct gctgcggctg agccagcagg
     4441 gcgcggatga gaacgccaaa gcgtccgagg ggcagggcgg cgggacggaa tacgagccgt
     4501 cctcgcacaa gtgggatgtg gcgagagaat ggggtgtcga tccggacgag ctgatgtggg
     4561 atttcagaaa aaacggttat gttctcgagg aggattttat taaatcgctt aagggcgacg
     4621 gtgaggtagc ggccattgcc ggcgctgacc cttattacgg acccgtgcgg agcgccgagc
     4681 cgcgggaaga ggaaaagacg caaccagagg aggaaaactc gggaagagaa aaaacaaagg
     4741 gaaaagatca actacggcgt ttaaattatt ccattttgga agagaatccg aaagtaaacg
     4801 atttgtcgaa agaggaattg caggacatac tgctcgactt tcagataaat attcaaaaat
     4861 ttaaccccta tacagatccc cccaaaaaat atgtaaagca gaatgggaaa ggagattgga
     4921 tccctgctga aaaaagtgaa gtgcgtgcgg cgatgttccc cgatccgtat gatccggtgc
     4981 aaaagtttca gtttatggat gtgcgggttg atccggaaga ggggtcgggg cttaccgctg
     5041 agcaaatcga tgactattta agccaaaata caaatgcgaa acttttccgt ggacatggac
     5101 aggattttat cgatgcggaa aaagcatccg gcatcaacgc cgcttttctg gtggccagcg
     5161 ccatctgcga gcagggggag ttgtcgaaag gaacgtcgtc ggctgcccca ggatttttca
     5221 atgtgtatgg gatcggggcc acagatggag aagcccagtt gggaggggga gaaacggccc
     5281 ggaaatatgg gtggacctcg ctctcttatg cgataaaggg aggcgcatat atattgcgca
     5341 aagattgggt ggatgcaggc caggcgacgg cttatgatat gcgctggaac ccatcgcacc
     5401 caacccagca ctggtattcc accgcgatca ttgccgccag cgaaagagca aagtatatgg
     5461 agggaattta tagttattgc ctggacgggg atacaaccgg gctggtattc acttatgatg
     5521 accgctattt gtaaacgaaa agggtaagat gaaaatgaga gacaggatag ccacaatagt
     5581 gttggcctgc gtgttgttct gcaccgctct ggccggatgc ggggccgcga cgggtttgtc
     5641 cgaaagcgct tctgttccca tttcggacgt tagtccccta tcttccggga cggtgagtcc
     5701 cacaccaacg ccgactttac gaatcgatcc cagctgcccg ccggaagaga tcaactttac
     5761 cccggaagag gcattgctct attttgcgtg gctgtactgg ggtaaagaag aggggcggga
     5821 ttttgcagcc acagagcggg aggaccttta tatcggcggt tatcccggcg agggcataga
     5881 cccgcgggag gggtcctatc aggcgacgat tgcagatgat atcatgtatt gggagggcga
     5941 gttcctctat acggtttatt tttccgtcgt ttatccggcc cggccgtcag gtgaagcgtt
     6001 gggaatgttc tttatcaacg taaataacct taagatgtgg tatgactttc tgccggacga
     6061 aggaaaaata aacagagcgg tggagcgttc gcttctggta gaatttttgc agcatatcgg
     6121 aaaagaagac ctgacagaag aggtgctggc gcagtacgag gaggacgaaa acgggacgct
     6181 ttggccctac ggcaggccga cggggacccc ggagcccaca gcgccgccaa gaaaaacccc
     6241 ggcgcccaca agtacccccg acccggcgtg gggcaaaagg gtgggtgtag atgagctggc
     6301 ggcgctggag tcccaagaaa aggccgtgcg ctacagtaag gaggaatgtt tggagatggc
     6361 ggcccggacg ctggcggcag gcggcgcggt ggagaccggt ttaccgggca gcctcgcaac
     6421 gtttccttgc gtggagtacg ccggagcgcc gggctgccgt ttgatcttgg tgccggggat
     6481 gttttggagc ccgaaaaatg agccctgggc ttatggatat gagtttatga tctacgtaga
     6541 agatgcgagc cagctgcggg agggcgcggt gccgccgccg gagggaccgg gaaaagaata
     6601 ccagacggaa gaaaatg
//
LOCUS       DBMDYA010000051         6560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_2996_length_6560_cov_1.350054, whole genome shotgun sequence.
ACCESSION   DBMDYA010000051 DBMDYA010000000
VERSION     DBMDYA010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_6560_cov_1.350054"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(336..1286)
                     /locus_tag="ACZPFK_02170"
     CDS             complement(336..1286)
                     /locus_tag="ACZPFK_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1405148.1"
                     /translation="MLYYLGIDIGGTKTAAGLLDSQGALLAEAEIPTEVSAGPEQIAQ
                     NTARLARSLLARSAGGEVPYVGVGCPGSIDGRRGVVKYANNLNFEEVPLAGMLERELG
                     LPVVLENDANCAGLGEYFALPDPDAVESFFIVTLGTGVGTAFVWNGRLFTGFNGAAPE
                     MGHCTLDAEGPVCDCGNRGCWEMYCAGHSLMRDARAAARNDRASLMWRLAGGVLEKID
                     GRVPFEAARRGDAAAQAVVRRYLYYFKAGVGNVINAFQPQVLTIGGGISREGTLFLDA
                     AREAIEEQSYCKTLEKTQVRAAVLGAKAGIYGAALLRRKP"
     gene            complement(1302..2297)
                     /locus_tag="ACZPFK_02175"
     CDS             complement(1302..2297)
                     /locus_tag="ACZPFK_02175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1405149.1"
                     /translation="MNFYLEALQDEHQLLLKALPGYRGAVERVGARIRALFAAGKARR
                     LLLAGMGSSWYAARSAAPALNGKGCFTVAMNCFELLTQTPGMVDAHTVLVLITQSGNT
                     PEAVRLAEYARGRAALTVAVLNEEQCAMRGLTDEELLLDMGRETRISNKTYYAQVALL
                     NLLAAAVTEGDLDAVEREIRRAVDWHEEYRSAQEKRTEPLLDIFRRSALIEALGDGPQ
                     GGAAQQAGLVLREMPLLQVACYSASDYSHGWFDIARPGYTAVFFCDRITALDERMMRH
                     CLEAGARVVLLAAEKAPLQHENLLEVPLPDQKGGLLPLYSIVPMYFAAGLLPERR"
     gene            complement(2388..3407)
                     /locus_tag="ACZPFK_02180"
     CDS             complement(2388..3407)
                     /locus_tag="ACZPFK_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1405150.1"
                     /translation="MKKIMAATLALELACSLLSGCGGTSSASPSAGGAAPASSGAAST
                     GGAAGGHTFAVVVHSTQSTYWQTLNAGAEAAAAESGDSIYFTAPINGSADINGQVDVM
                     QNCINQNVDGILLAAADVDALVPITEQAIDKGIPVVIVDCGLNTDRYESSIATDNRAA
                     AAEMAHKVAEDIGGEGQVAIVNYAAGMQTGSLREDGFREAMAEYPGITLLETQYYNND
                     TQKALEVTQNLLTANPDLKAIYAMNEFGIVGASRALMEKKNDQIKVYGFDFSDDVLPL
                     LEQGVCAGTMVQKPYDMGYLGVQSLVKVLAGESVEKDVDSGCVLATPENYKDEEVYKV
                     LYPMG"
     gene            complement(3423..4409)
                     /locus_tag="ACZPFK_02185"
     CDS             complement(3423..4409)
                     /locus_tag="ACZPFK_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405151.1"
                     /translation="MLNHGAAEGTAENTILNRGKGLLNQMKSFTALLLLGLVFAVMSP
                     NFLSVNNLVTVVQQSSNIAILAYAETMVIITGGIDLSLGSILGVSGVVVGKMILAGAP
                     VWSAVLGGILTGGLCGVVNGFVVSKMKLVPFIATLGMQNIARGVAYVLTNSLPVSGLS
                     EGLYFIGGGSVGIIPVPVIIMLVLAVGFAFLLKKCAFGRRVYSIGSNREAARLSGINV
                     GRTEMGVFAIGGLLAGLVGVILASRVLSSQPNAGVGYESDAIAAAIIGGTSPSGGSGT
                     ILGTVIGALTIAVLKNGLNLLQVNPFWQQIAIGIVIIVAVFIDNLRKKNNKK"
     gene            complement(4434..5921)
                     /locus_tag="ACZPFK_02190"
     CDS             complement(4434..5921)
                     /locus_tag="ACZPFK_02190"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405152.1"
                     /translation="MQQILSMNGIVKAFPGVKALKGVHFDLRAGEVHALVGENGAGKS
                     TLMKILTGSYQADEGEIVYNGGPVSIPTPRAAQEMGINIVHQEIHLMPDLTVAENIFV
                     GFEPRNRFFRAAYDFREMNRRAAAVLAEIGLAVPPDTITGKLSVAQQQMVAIARVVEL
                     NSRIVVLDEPTAALAAAEVDALFAIIQKLKRKGIGIVYISHRLEELDVVADRVSVFRD
                     GSYITTLDYRDTSKEELISLMVGRELTDQYPNHHAARGEELLRVNRLTTRQGLDVSDF
                     VLHRGEIVGIYGLVGAGRTEFARALFGADRAEQYDVLLRGRPLKVRDTAQAVRSGLGY
                     LTEDRKRDGLALGLDVENNITLANLEGLSRFGVMQQKACEENAEKYVEELKIRTPSTR
                     QLARFLSGGNQQKVIIAKWLSRNAGILIFDEPTRGIDVGARKEVYELLNRLVEDGVGV
                     IVISSDLPEVIGVSDRILVMHDLHFTGGLDKGEATQEKLLALAVQ"
     gene            complement(5936..>6560)
                     /locus_tag="ACZPFK_02195"
     CDS             complement(5936..>6560)
                     /locus_tag="ACZPFK_02195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1405153.1"
                     /translation="ANHCGAKWDGVNAIEKMAKIIAGLQEAERNWALTRRHPYLPPQS
                     ISIDTIQGGTASTIVPDLCKLEVIVHFQPHHEPGYRWMGEKYDQEVREVIRRVALSDP
                     WLEAHPPKVTLYQLGSSCDIGVDHPICRALSEKTVEITGKKPGCKGLVSGADGRLLNN
                     YADTPTVHFGPGSMEIAHTVDEFVPVQEYLDCIKIFAATMMDWCGIS"
BASE COUNT         1086 a         2303 c         1942 g         1229 t
ORIGIN      
        1 ctatctggag gacatcgcca cagcccccca cacgctggcg gagggctcct gtctggagag
       61 gctgatccgg gggctgcgat agcggcaggt ccgcagggcc atcgcatccc ggggagcgtg
      121 gacggcgccc cttgcccctc cgggcccttt tggccccctt gccggaggct ccttttttct
      181 attcactttg tattcaaaaa ggccggggac agcgccgtcc ccggcctttt ctctttctcc
      241 gtcttttcag gccccatggg ggccatgccg tgctctttgg gcccccaggc ccttgtcccg
      301 ggagggaaag caccccccag acggcgggtg tcctctcacg gtttccgtct cagcagggcc
      361 gcgccgtaga tgcccgcctt tgcgcccagc acagctgcgc gcacctgggt cttttccagt
      421 gttttacagt aggactgctc ctcgatcgcc tccctggccg cgtccaggaa gagcgtcccc
      481 tcacggctga tgccaccgcc gatggtcaag acctgcggct ggaacgcatt gatcacattg
      541 cccacgcccg ctttgaaata gtacaggtaa cgcctcacca cggcctgcgc ggcggcgtcc
      601 ccgcggcggg ccgcctcaaa cggcaccctg ccgtcgattt tttccagcac gccccccgcc
      661 agccgccaca tcaggctggc ccggtcgttc cgggccgccg cgcgggcgtc ccgcatcagg
      721 gagtggccgg cgcagtacat ctcccaacag ccgcggttgc cgcagtcgca gaccggcccc
      781 tcggcgtcca gcgtgcagtg ccccatctcc ggcgcggcgc cgttgaagcc ggtgaagagc
      841 ctgccgttcc agacgaacgc cgtgcccacg cccgtgccca gggtgacgat aaaaaagctc
      901 tccaccgcgt ccgggtccgg cagggcgaaa tactcgccca agcccgcgca gttggcgtcg
      961 ttctccagca ctacgggcag gcccagctcc cgctcgagca tccctgcgag cggcacctct
     1021 tcaaagttca aattgtttgc atatttgacc acgccgcgcc tgccgtcgat ggagccgggg
     1081 caccccaccc ccacataggg cacctccccc cccgcgcttc gtgccaaaag gctgcgcgcc
     1141 aggcgcgcgg tattttgggc gatctgctcc gggcccgcgc tcacctcagt ggggatctcc
     1201 gcctcggcga gcagcgcgcc ctggctgtcc aacaacccgg cggccgtctt tgtgccgccg
     1261 atatcaatgc cgagataata caacataccg tccccccttg tctaccgccg ctcaggcagc
     1321 aggcctgcgg caaaatacat cggcacgatg gaatagagcg gcaaaaggcc gcccttttgg
     1381 tccggcagcg ggacctccag caggttttcg tgctgcagcg gcgccttttc cgcagccagc
     1441 agtaccaccc gggcccctgc ctccagacag tgccgcatca tcctctcatc cagcgcggtg
     1501 atccggtcgc agaaaaagac cgccgtatac cccggccgcg cgatatcaaa ccagccgtgg
     1561 ctgtagtcgc tggcgctgta gcaagccacc tgcagcagcg gcatctcccg cagcaccagc
     1621 cccgcctgct gggccgcgcc cccctgcggg ccgtcgccca gcgcctctat cagcgccgag
     1681 cggcggaaaa tgtccaacag cggctcggtg cgcttttcct gcgcagagcg atattcctcg
     1741 tgccagtcca ccgcccggcg gatctcccgc tccacggcgt ccaggtcccc ctccgtcacg
     1801 gcggcggcca gcaggttgag cagcgccacc tgcgcgtaat aggtcttgtt ggagatgcgc
     1861 gtctcgcgcc ccatgtccag caaaagctcc tcatccgtca ggccccgcat ggcgcattgc
     1921 tcctcgttga gcaccgccac cgtgagggcg gcccggcccc gggcatactc cgccagccgc
     1981 accgcctcgg gcgtattgcc cgactgggtg atcagcacca gcaccgtgtg cgcgtccacc
     2041 atccccggcg tctgggtgag cagctcaaaa cagttcatcg ccacggtgaa acagcccttg
     2101 ccgttgagcg ccggcgccgc gctgcgggcc gcataccagg acgagcccat gccggccagc
     2161 aagaggcggc gggcctttcc cgcggcaaag agggcgcgga tgcgggctcc cacccgctcc
     2221 accgcccccc gatagccggg cagcgccttt agcagcagct ggtgttcgtc ctgcagcgct
     2281 tccaaataaa aattcatcaa ttccctccga tcctcaaaaa atgggccccg gcatcccgta
     2341 aaaggcagtg ccggggcccg ttgcttctct ccgcttttcc cggggcttta acccatgggg
     2401 tacaatacct tatagacttc ctcgtccttg taattctcgg gcgtggccag cacacagccg
     2461 gagtcaacgt ctttttccac gctctcgccc gccagcacct tcaccagcga ctgcacgccc
     2521 agatagccca tgtcgtaggg cttctgcacc atggtgcccg cacagacgcc ctgctccagc
     2581 agcggcagca cgtcgtcgga aaagtcaaag ccgtagacct tgatctggtc gttctttttc
     2641 tccatcaggg cccggctggc gccgacgatg ccaaactcat tcatggcgta gatggctttc
     2701 agatccgggt tggcggtcag caggttctgg gtgacctcca gggccttttg ggtgtcgttg
     2761 ttgtagtact gcgtctccag cagggtgatc ccggggtact cggccatggc ctcgcggaag
     2821 ccgtcctcgc gcagcgagcc ggtctgcatg ccggcggcgt agttgacgat ggccacctgc
     2881 ccctcgcccc cgatgtcctc ggcgaccttg tgcgccatct cggcggcggc cgcccggttg
     2941 tccgtggcga tggagctctc atatcggtcg gtgttcaggc cgcagtccac gatgaccacg
     3001 gggatgccct tgtcgatggc ctgctcggtg atggggacca gcgcgtccac atccgccgcg
     3061 gccagcagga tgccgtccac attctggttg atgcagttct gcatcacgtc cacctggccg
     3121 ttgatgtcgg cgctgccgtt gatcggcgcg gtgaaataga tgctgtcgcc ggattcggcc
     3181 gcggccgcct ccgcgcccgc gttgagggtc tgccagtagg tggactgggt cgagtgcacc
     3241 accaccgcaa aggtgtgccc acccgccgcg ccgccggtgg aggcagcgcc ggaggaggcg
     3301 ggggccgccc cgccggcaga cggggacgct gaggaagtgc cgccgcaccc ggagagcagg
     3361 ctgcaggcga gttccagtgc cagggttgcc gccatgattt ttttcatctt tccatttctc
     3421 ccttactttt tgttgttttt ctttctcaga ttatcaataa ataccgcgac gatgatcacg
     3481 ataccgattg cgatctgttg ccagaacggg ttgacctgca gcagattgag gccgttcttg
     3541 agcacagcga tggtcagcgc gccgatgacc gtgcccagga tggtgcccga gccgccagag
     3601 gggctggtgc cgccgatgat cgcggccgcg atggcgtcgc tctcatagcc cacgcccgcg
     3661 tttggctggg acgagagcac ccgggaggcc agaatgacgc ccaccaggcc ggccagcagg
     3721 ccgccgatgg cgaacacgcc catctctgtt ctgcccacgt tgatgccgga cagccgcgcc
     3781 gcctcccggt tgctgccgat cgagtaaacc cggcggccga acgcgcactt tttcagcaga
     3841 aaagcgaacc ctaccgccag caccagcatg ataatgaccg gcaccgggat gatgcccacg
     3901 ctgcccccgc cgatgaagta aaggccctcg gagaggccgg ataccggcag cgagttggtg
     3961 agcacatagg ccacgccgcg ggcgatgttc tgcatgccca gggtggcgat aaagggcacc
     4021 aatttcatct tggagaccac aaacccgttg accacgccgc aaaggccgcc ggtcaaaatg
     4081 ccgcccagca ccgcgctcca caccggtgcg cccgcgagga tcatcttgcc caccaccacg
     4141 ccggacacgc ccaaaatcga ccccagagag aggtcgatgc ccccggtgat gatgaccatt
     4201 gtctccgcat aggccaggat ggcgatgttg gacgactgct gcaccaccgt caccaggttg
     4261 ttcaccgaga gaaaattggg gctcatcacc gcaaagacca gccccagcaa gagcagggcg
     4321 gtaaaggact tcatctggtt gagcagccct ttgcccctgt tcaagatcgt attttcagcc
     4381 gtgccttcgg ccgcgccatg attcaacatt gcagaccctc ccaggttttg tttttattgc
     4441 accgccagcg ccagcagctt ttcctgcgtg gcctcgccct tgtccagccc cccggtgaaa
     4501 tgcaggtcgt gcatgaccag gatgcggtcc gacacgccga tgacctcggg caggtcggag
     4561 gagatgacga tgacgcccac cccgtcctcc acaagccggt tcaacagctc gtagacctcc
     4621 ttgcgcgcgc ccacgtcgat gccccgggtc ggctcgtcga agatcagaat accggcattg
     4681 cgggagagcc atttcgcgat gatgaccttt tgttggttgc cgccgctgag aaaacgggcc
     4741 agctgccggg tggagggggt gcgtatcttc agctcctcca catatttttc ggcgttttcc
     4801 tcgcaggctt tctgctgcat cacaccgaac cgggagagcc cctccagatt tgccagcgtg
     4861 atgttgtttt ccacatccag gcccagcgcc aggccgtccc gcttgcggtc ctcggtgaga
     4921 tatcccaggc cgctgcgcac cgcctgcgcc gtgtcgcgca ctttcagcgg ccgtccgcgc
     4981 agcagcacgt cgtactgctc cgcccggtcc gcgccaaaca gggcgcgggc aaactcggtg
     5041 cgcccggcgc ccaccaagcc gtagatcccc acaatctcgc cgcggtggag cacgaagtcg
     5101 gacacgtcca gcccctgccg ggtggtcagc cggttcaccc gcagcagctc ctcgcccctt
     5161 gcggcgtggt ggttcggata ttggtcggtc agctcgcggc ccaccatcag cgagatcagt
     5221 tcctccttgc tggtgtcccg gtaatccagc gtggtgatgt agcttccgtc ccgaaaaacg
     5281 ctcacccggt ccgccaccac gtccagctcc tccagccggt gggagatgta gacgatgccg
     5341 atccctttcc gcttcagctt ttggatgatg gcgaagagcg cgtccacctc cgccgcggcc
     5401 agcgctgcgg tgggctcgtc cagcaccacg atcctgctgt tgagctccac cacccgcgcg
     5461 atggccacca tctgctgctg ggccacgctc agcttgccgg tgatcgtgtc cggcggcacg
     5521 gccagcccga tctccgccag cacggccgcg gcgcgccggt tcatctctct gaaatcatag
     5581 gcggcgcgga aaaagcggtt gcggggctca aaccccacaa agatattttc cgccaccgtg
     5641 aggtccggca tcagatggat ctcctggtgc acaatgttga tccccatctc ctgcgcggcc
     5701 cgtggggtcg ggatcgagac cgggccgccg ttgtagacga tctccccctc atccgcctga
     5761 tagctgccgg tgaggatctt catcagggtg gatttgcccg ccccgttctc ccccaccagc
     5821 gcgtgcacct cgccggcgcg cagatcgaaa tggaccccct tgagcgcttt tacgccggga
     5881 aacgctttca cgatcccgtt catcgacagg atttgctgca ctccgcttca ccaccttaag
     5941 aaattccgca ccagtccatc atcgtcgccg caaaaatttt gatgcaatcc agatattcct
     6001 gcaccggcac aaactcgtcc accgtgtgcg cgatctccat cgaccccggc ccgaaatgca
     6061 cggtgggggt gtcggcgtag ttgttcagca ggcgcccgtc cgcgccgctt accaggccct
     6121 tgcagccggg ctttttgccg gtgatctcca ctgttttttc cgacagggcg cggcagatcg
     6181 gatgatccac cccgatgtcg caggaactgc ccagctggta gagggtcacc ttgggggggt
     6241 gcgcctccag ccaggggtcg ctgagggcca ccctgcggat tacctcccgc acctcctggt
     6301 catacttttc gcccatccag cggtaacccg gctcgtgatg cggctggaag tggacgatca
     6361 cctccagctt gcacaggtcg ggcacgatgg tggaggcggt gccgccctgg atggtgtcga
     6421 tgctgatgct ctggggcggc agataggggt ggcggcgggt cagcgcccag ttgcgctccg
     6481 cctcctgcag gccggcgatg atctttgcca tcttttcgat ggcgtttaca ccgtcccatt
     6541 tggccccgca gtggtttgcc
//
LOCUS       DBMDYA010000052         6543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3002_length_6543_cov_1.421608, whole genome shotgun sequence.
ACCESSION   DBMDYA010000052 DBMDYA010000000
VERSION     DBMDYA010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_6543_cov_1.421608"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="ACZPFK_02200"
     CDS             complement(<1..712)
                     /locus_tag="ACZPFK_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR1405154.1"
                     /translation="MQEKRRVRFDPELGVEAYCLGGMAQKFPLHFHDYYVVGMIESGR
                     RRMLGGGSCVLEPGDMVLFNPGDAHGCEQVGGPFSYRALNIGAKTMREAGRQITGSGL
                     PPRLGPRVVRGSPLAQQLCAVQALILRGEQGLRKEEAFWLFLGRLLCAHAQPAGPRAA
                     ESGGVAAAAAYLEAHVQQQVTLDALAGAAGLGKYRLLRAFARERGISPYRYLEALRVA
                     RAQALLQQGMPPAEAALEV"
     gene            complement(1359..1571)
                     /locus_tag="ACZPFK_02205"
     CDS             complement(1359..1571)
                     /locus_tag="ACZPFK_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR1405155.1"
                     /translation="MHTGTVKWFNEQKGFGFVSDDDGSGDVFVHFSAIVAEGFKTLAE
                     GQKVSFDVEPDPKNSSKMRAVNVTAI"
     gene            complement(2804..3064)
                     /locus_tag="ACZPFK_02210"
     CDS             complement(2804..3064)
                     /locus_tag="ACZPFK_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405156.1"
                     /translation="MPDAVKMHNIDVAEFTEIINSCVGDVYMVTPDGDRLNLKSKLCQ
                     LIGFTRLIEGGTLAEAELVCEDPADESKLFRFNLYGKDAKEK"
     gene            complement(3118..4098)
                     /locus_tag="ACZPFK_02215"
     CDS             complement(3118..4098)
                     /locus_tag="ACZPFK_02215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1405157.1"
                     /translation="MEQQRRTPVRGRREDILSRMPGGAFCYELREGAPGPVTFISEGM
                     LRLLECTERQFRQEYGGEIRRLLCPLGAQPPARACDQGREPDNFSCRIRTRGGRLRWL
                     AVSRRVCRDEDGVLRAYVTAVDSTAEHEATERLQQEREDYRRRAQKDLLTGVYNKVTA
                     ERLVEKLLASGLGGALYLVDVDDFKTVNDTFGHLCGDEALAGVSGCLRGALRHGDVVG
                     RVGGDEFLLYLPGVDSVEAARSAGLKVLEALEQLSVGPQGRGRLSVSIGGVLCDQTLQ
                     EYRSAYRRADEALYLAKARGKNCLELWRPDGGKIFSPRAQKAGGCQGAGV"
     gene            complement(4542..5402)
                     /locus_tag="ACZPFK_02220"
     CDS             complement(4542..5402)
                     /locus_tag="ACZPFK_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HIR1405158.1"
                     /translation="MERPSLALTHAGHFHADDVFSAALLREVWPGLEIRRAYEVPEGF
                     DGLAFDVGGGAFDHHGPERKLRQNGMPYAAFGLLWQELGEQLVGAEQAKWLDEHFIQP
                     LDKDDNTGCGDSLADAIGGFNPVWDSDADADACFWRAEAFAAQVLKNRLEGARAVNRA
                     RSLVEKALAKMKDNVVVLPRFAPWKNTLKGSPAELVVYPSQRGGFSAQGVPIPDDPKH
                     RLKCRFPESWAGLRAPQLERVSGIRGLRFCHASGFLVSANTLESALAACRAARAEAEE
                     KRRAEGEARA"
     gene            5889..>6543
                     /locus_tag="ACZPFK_02225"
     CDS             5889..>6543
                     /locus_tag="ACZPFK_02225"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1405159.1"
                     /translation="MREEFARSALLLGEEAEARLARASVAVFGIGGVGGHAAEAVARA
                     GVGRIALFDADTVSASNINRQIVALHSTLGLPKAQVMADRIRDINPLCRVEPHIVFYG
                     EENAAQFPLGEYDYIIDAVDTVSAKLLLIQRAGEAGVPVISAMGCGNKLDPSRFEVAD
                     LSRTSVCPLARVMRRELKARGVTGVKVVYSQEPPLAPAAQGQEAAEAPAPGRRAVPGS
                     "
BASE COUNT          988 a         2438 c         2098 g         1019 t
ORIGIN      
        1 ccacctccag cgccgcctcg gcgggcggca tgccctgctg cagcagcgcc tgggcgcggg
       61 ccacccgcag cgcctccaga tagcggtagg gcgagatgcc ccgctcgcgc gcaaaggccc
      121 gcagcagccg gtatttcccc aggcccgcgg ccccggccag cgcgtccagg gtcacctgct
      181 gctggacatg ggcctccaga taggcggccg cggcggccac cccgccgctc tcggcggcgc
      241 ggggcccggc gggctgcgcg tgggcgcaga gcagccgccc caaaaagagc cagaacgcct
      301 cctccttgcg caggccctgc tcgccccgca ggatcagcgc ctgcacggcg cacagctgct
      361 gcgccagcgg gctgccgcgc accacccgcg gccccagccg gggcggcagg ccgctgccgg
      421 tgatctgccg ccccgcctcc cgcatcgtct ttgcgccgat gttcagcgcc cggtagctga
      481 aaggcccgcc cacctgctcg cagccgtggg cgtcgccggg gttgaacagc accatatccc
      541 ccggctccag cacgcaggag ccgccgccca gcatccgccg ccgcccgctc tcgatcatgc
      601 ccaccacata atagtcgtga aaatgcagcg gaaacttctg cgccatgccg cccaggcaat
      661 aggcctccac gcccagctcc gggtcaaagc gcaccctccg tttttcctgc accggtccac
      721 ccccctggct agagatagtt tagcgcgccc cgcgcgccgc gtcttgtacg atattgcgcc
      781 ccaggagagg gcccccggcg ctggcgaagc cgttccggcg cggtgaagcc ctgctgaaag
      841 gatggagcgc gcggcgcagc atcttaaaag aggcaagcgc cgcatgacgc cgcctctccc
      901 catacggccc gcggccgcca gctctgctca gagagggcgg acgcgcgccg acgcaggcgg
      961 cggagcaggc ggtaacgccg ctattttcca cgaaacataa gaggcctggg gcgcgctgtc
     1021 ccggccgccc cggcgagaac aggcggcggg cggaggatcg agccgcaaaa tgaagagaaa
     1081 ggccgttctt ttctgtgaaa cacaaaaggc acggggcacg ttgtttccgc cgcctcgaaa
     1141 agggaagcgg cgcgccgaag ccctgccgga gcggcggtgg gcgcggcgtg atgtcttaaa
     1201 gagagaatcg ctgcatgacg ccggagcaaa cgccctcaaa cggcccgcgg caggcagagc
     1261 tgcgggccgc agggcagaga ggggccgccc cggcgggtgg cccgcggtat aaaaaaacgc
     1321 cctcccacag agggagggcg ttctgttagc cgtgtcgatt agatcgcggt cacattgaca
     1381 gcgcgcattt tgctgctgtt cttggggtcc ggctccacgt cgaagctgac cttctggccc
     1441 tcggccaggg tcttgaagcc ctcggccacg atggcggaga agtgcacgaa cacgtcgccg
     1501 ctgccgtcgt cgtcagagac gaagccaaag cctttctgct cattgaacca tttcacagta
     1561 ccggtatgca tagtagtgcc tccaaaaatt ttttcgcttc tgtcgtacaa aataaaagcc
     1621 gcatccggcg taaatcatca gtagaacaac gacctacaca gcatgtggcg tcaattggta
     1681 ctatcagaat acaagagtaa gtatatcaca gcccgggcaa aagtacaact acccgggcaa
     1741 aattttttta tttttccatg gaaagacctg ccagccgcgc ccgggcggca aaaagcttct
     1801 ttttttgtga gaaaaagggg gtccgcgccg tctgtggaag acctcgcgcg gccgctcatc
     1861 cgcccccgcc gcagcgcagg cggcgctccc atctgcggca tccccttttt accaggcccg
     1921 tcagggccag agcgcccgcg gcgcagagca gtgcagtcat cctccgccct cctttccggc
     1981 ggtcgcccgg cgggccccgc gggcctgtgc cgcggacgta cgcctatagg ctgaaaaacg
     2041 gggagacggc cgcatccgtt gcccgccccc cggtcgtttt gggagggccg cggccggaga
     2101 gccccaccgg ccgcggcgtt ttatggggtg gcgcagagaa cccgcgcccc cgctgagaag
     2161 cgcgccgggc ggcatggcgc aaccgaccgg cggggaggac gccatggccg agcgccctcc
     2221 gcccccgcct ccacctcgcg ggggagggag gattcacctc tctagttagt ttaaactaac
     2281 ctaaagctat cataccatgt ctctccccgg ccgtcaagag gggaacgagc gcccacccat
     2341 ggcatttttt gcctgccatc gcttcggccc tcttccaaag ggggtgaaag acggctctcc
     2401 cctccctgcc cctgcttttg cccctctccc cttgccgggg gcggcggggt ctgcacgcct
     2461 ttggccccgt gccccgccga agacggcgca cccccggctt caccgccgtc accgacgcat
     2521 tctctcggcc tcccctgcgg ccgccgcaaa gcgggagtcc cctccccttg ccgggggcgg
     2581 cgggcttgtg cgtctccggc tccgtgtccc gtcggggaca gcgcatcttc ccagccctct
     2641 cccggcctca tccccgtccc gcgtctgcgc ttcctctcag cccctccccc gccccgcggc
     2701 ggcgcagctc cggccgcccg cccacgggaa aggcggcaaa aaaaagcagc cgcttttgcg
     2761 gctgcccctt atcgtttcgg cccgccctct gcgcggggcg cggtcatttt tcctttgcgt
     2821 ccttgccgta gagattgaag cggaacagct tgctctcatc cgccgggtcc tcgcagacca
     2881 gctcggcctc ggccagggtg ccgccctcga tcaggcgggt gaagccgatg agctggcaca
     2941 gcttgctctt caggttcagg cggtctccat ccggggtcac catatacacg tcccccacac
     3001 agctgttgat gatctcggtg aactccgcca cgtcgatatt gtgcatcttt accgcatccg
     3061 gcatcctaca gacctccgtt tctttttggg gctgggcccc gccgtcctct gtcttcatta
     3121 tacccccgcg ccctggcatc caccagcttt ttgcgcgcgg ggagaaaaaa tttttccgcc
     3181 gtcgggccgc cacagctcca ggcagttctt gccccgggcc ttggcgaggt acagcgcctc
     3241 gtcggcccgc cggtaggcgc tgcggtactc ttgcagcgtt tggtcgcaga gcacgccgcc
     3301 gatgctcacc gagaggcgcc cccgcccctg cggccccacc gagagctgtt ccagcgcctc
     3361 cagcaccttg agccccgcgc tgcgggccgc ctcgacgctg tccacgccgg gcaggtagag
     3421 caaaaactcg tcgccgccca cccggcccac cacgtcgccg tggcgcaggg ccccgcgcag
     3481 gcagcccgag accccggcca gcgcctcgtc gccgcacagg tggccaaagg tgtcgttgac
     3541 ggtcttgaaa tcgtccacgt ccaccagata cagcgcgccg cccaggccgg aggccagcag
     3601 cttttccacc aggcgctcgg cggtgacctt gttgtaaacg ccggtgagca ggtctttctg
     3661 ggcgcggcgg cgatagtcct cccgctcctg ctgcagccgc tcggtggcct cgtgctccgc
     3721 ggtgctgtcc accgccgtca cataggcgcg cagcacgccg tcctcgtccc tgcacacccg
     3781 gcgggacacg gccagccagc gcaggcggcc cccgcgggtg cggatgcggc agctgaagtt
     3841 gtccggctca cggccctggt cgcaggcccg ggccgggggc tgcgccccga gcgggcagag
     3901 cagccgccgg atctcgccgc cgtactcctg ccgaaactgc cgctccgtgc actcgagcag
     3961 ccgcagcatc ccctcgctga taaaggtcac cggccccggc gcgccctccc gcagctcgta
     4021 acagaacgcg ccgccgggca tgcggctgag gatgtcctcc cgccgccccc gcacaggtgt
     4081 acgccgctgc tgctccaatc ccgctctact cccctccggc cgaccgccgc aattttgccc
     4141 tttcagtata ccattttatg gaaaccgccg caacagtttc gagccgctgc ggcagcatgc
     4201 gacccccgcg ccgtgaccgg ccgggcgcgc ccgcccccgc ggggccgccc tgctctatcg
     4261 caagaccttc accgggccgt cttatcgggg ccgctaagcc ctgtcgcaaa cccgcgatgc
     4321 ccggggccgt cctgtcgcgg ggccgccgtc gagaccgcca taccctatcg caagaccgcc
     4381 atcccctgcc gcgggaccgc cctgccttgt cgcaaggccg ccctatcgag accaccgtga
     4441 aaaaatcccc cgccagcact tccggcgccg cgcccggcgg cacgcgtcca tcgcagcagc
     4501 atccaacgca gccgccgcag ctaccgcagc cgcccggccg ctcaggcccg ggcctccccc
     4561 tcggcgcgcc gcttctcctc ggcctcggcg cgggcggcgc ggcaggcggc cagggccgac
     4621 tccagcgtgt tggcgctcac cagaaagccg ctggcatggc aaaagcgcag cccgcggatg
     4681 ccggacaccc gttccagctg gggggcgcgc agcccggccc agctctcggg gaagcggcat
     4741 ttcagccggt gtttggggtc gtcggggatg ggcaccccct gggcgctgaa gccgccccgc
     4801 tgcgaggggt agaccaccag ctcggcgggc gagcccttga gggtgttttt ccagggggcg
     4861 aagcggggca gcaccaccac gttgtctttc attttggcaa gggccttttc caccagcgac
     4921 ctggcccggt tgacggcgcg ggcgccctcc agccggtttt tcagcacctg ggccgcaaag
     4981 gcctcggcgc gccaaaagca ggcgtccgcg tccgcgtcgc tgtcccacac cgggttaaag
     5041 ccgccgatgg cgtcggccag cgagtcgccg cagccggtgt tgtcgtcctt gtccagcggc
     5101 tggatgaagt gctcgtccag ccatttggcc tgctcggccc ccaccagctg ctcgcccagc
     5161 tcttgccaga gcaggccgaa cgcggcgtag ggcatgccgt tctgccgcag cttgcgctcc
     5221 gggccgtggt ggtcgaaagc gccgcccccc acgtcgaacg ccaggccgtc gaacccctcc
     5281 ggcacctcat aggcccggcg gatctccagc ccgggccaga cctcccgcag cagcgcggcg
     5341 ctgaacacgt cgtcggcgtg aaaatggccc gcatgggtca gggcaaggct cggtctctcc
     5401 atgatggttt ccccctgtat cgcttgatgt cgcttgatgt cgcttgatgt tgttcgatgt
     5461 cgcttgatgt tggttgatgt cgctcgcgcc ccgccctccc ggcccgggcc gcggggcgct
     5521 ttttgggcgg acgcgccggg tgttcccgac ttctacccac cgccgaggac gggcaaagcg
     5581 cagccaaaga ggcggaggcc gcgccgcctt ttcccccgcc cgccggggac gggccggatg
     5641 ccgcgcctcc ccgggccttt gcccgccggg gcggggcacg ggggccaaag tttttcccgc
     5701 tcttttatta tacaccatcc ggccgcccgg cgcacggccc cgcccccggc aagcgcactg
     5761 gacgctcgcc agcccagcca gggacgcccg ggcgctgacg gacggccccg cccccggcgg
     5821 gcgcacttga cgctgcccgc gccgccgcct ataatcttga cagagggctc ggagaggagg
     5881 ggtaccccat gcgggaagaa tttgcgcgct cggcgctgct gctgggcgag gaggccgagg
     5941 cgcggctggc ccgggcgtcg gtggcggtgt tcggcatcgg cggggtgggc ggccacgcgg
     6001 ccgaggccgt ggcccgcgcc ggggtggggc gcatcgcgct ctttgacgcc gacacggtca
     6061 gcgcctccaa catcaaccgg cagatcgtgg cgctgcacag cacgctgggc ctgccgaaag
     6121 cccaggtcat ggccgatcgc atccgggaca tcaaccccct ttgccgggtc gagccgcaca
     6181 tcgtctttta cggcgaggag aacgcggcgc agttccccct gggggaatac gactacatca
     6241 tcgacgcggt ggacaccgtc tcggccaagc tgctgctcat ccagcgggcc ggggaggcgg
     6301 gggtgccggt gatcagcgcc atgggctgcg gcaacaagct ggacccctcc cgctttgagg
     6361 tggcggacct ctcgcgcacc agcgtctgcc cgctggcccg ggtgatgcgg cgggagctga
     6421 aagcccgcgg cgtcaccggg gtcaaggtgg tctactcgca ggagccgccc ctcgcccccg
     6481 cggcacaggg gcaagaggcg gccgaggcgc ccgcgccggg ccgccgggcc gtgccgggca
     6541 gcg
//
LOCUS       DBMDYA010000053         6514 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3017_length_6514_cov_1.619991, whole genome shotgun sequence.
ACCESSION   DBMDYA010000053 DBMDYA010000000
VERSION     DBMDYA010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_6514_cov_1.619991"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPFK_02230"
     CDS             complement(<1..213)
                     /locus_tag="ACZPFK_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1405160.1"
                     /translation="MIANYHTHTARCLHAAGAEREYVERALQGGCRVLGFSDHTPYPF
                     PAPYVSGFRMRMDQLEGYVRTVLDLRA"
     gene            complement(210..1028)
                     /locus_tag="ACZPFK_02235"
     CDS             complement(210..1028)
                     /locus_tag="ACZPFK_02235"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1405161.1"
                     /translation="MRTVQYELMRPGELLAEKERFSVAYLPVGPLEWHGPHMPFGTDP
                     LDAQAVARGVAEKIGGVVFPTLYCGTERARTPQELRCMGFEDDDLYVVGMDVPATSVK
                     SCYFPEEAFGMILREYLLRIVEQRFRLVVVVNGHGASGQLAAGERLAKEFTHTTPATV
                     LFTRAMQKLEEDDQRLGHANISETSMQMYISGDSVDLDKLPPREVPLKTCEWGIADAL
                     EFQGRGNAAHTVEQDPRDATALLGRRYVENGIERISAQVLAAYAELPQPPERGR"
     gene            complement(1126..>1894)
                     /locus_tag="ACZPFK_02240"
     CDS             complement(1126..>1894)
                     /locus_tag="ACZPFK_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1405162.1"
                     /translation="PEKYMTLIDTAIADNADAIVTCIPDQTMSQSVVDKCKDAGVPII
                     AVDDGLIDTDGNKIAPWFGIDAYNIGYTAGEWMADYAKKNDLLDDPSVGLLYMTMETV
                     SSCVPRTEGEKDAWKDKLGDALSDRTYEADYVSMSEEAYNSASAVVTAHPEIKTWLVM
                     TPSENGVLGCGAVLEEAGLDATSCVIALGCDEMSNQWADGNYSVCRASAYFSGKVVGK
                     EAITAVVEYLVNGTEIPAEYATPAVIVTPENYTDYVL"
     assembly_gap    1895..1904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1905..2182)
                     /locus_tag="ACZPFK_02245"
     CDS             complement(<1905..2182)
                     /locus_tag="ACZPFK_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405163.1"
                     /translation="MKKFVSLLLAGMMALSLVACGGSDSSSSAAPVSSTPDASSTAAG
                     GKVEALNIYGIYKSESTYFVNEAASIEKTLGELGEKYGFEATWHFNNC"
     gene            complement(2365..3360)
                     /locus_tag="ACZPFK_02250"
     CDS             complement(2365..3360)
                     /locus_tag="ACZPFK_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405164.1"
                     /translation="MNEKKFKLPISGNQLVLICALVGVTVIFSVLNPNYFTYTNFINI
                     LVSSTLIGLTSIGITFLIMTGGNDLSAGAVAAFAGVFVAWALKATSLPWPLLCLAAVA
                     IAAVAGLLNALMVTKLNIVPFIATLASQSVWRGFAYLVCGGKPVSVADKGFMAFGKSR
                     LGGDDGIPYTVILMVIFFIIFAYILAKTKFGRSIFAIGGNAEAARLAGINANRVKTIC
                     YVMTSMFAALAGIILAARMNSGQPAASPNLHFDAITAANLGGVSMVGGVGSIPSVVLG
                     VLLVQAFNSGLNMVGVSSYWQYVARGMLLFASLAIDFYRQRSRARRLLADSMKAL"
     gene            complement(3353..4861)
                     /locus_tag="ACZPFK_02255"
     CDS             complement(3353..4861)
                     /locus_tag="ACZPFK_02255"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405165.1"
                     /translation="MGNVIEFRNITKYFPGVKALDSISFTARGGEVLALMGENGAGKS
                     TLLKVLNGDYQQDMGDYLVNGEPCHFSSPREAVAAGIGVIYQERQIMLELTVGENIFL
                     GRLPTGFLGHIKMNEVHERAQKIIDEFGLDMRSTDKVKDLSVAYQQMVEIMKAYSREN
                     LKMIAFDEPTASLSDAEIDSLFRIIRKLRDEGKVIIYVSHRMAEIREVADRVAIFKDG
                     CFVGEAPIQEITDKQLIEKMVGRDLGDVFAALDKDKEIGEVVLEVKNLQSPYLKDASF
                     TVRAGEVVGFSGLVGAGRTEVVRAITGADPRTGGEVLLDGKPLVCKSPHDAIKNGVVM
                     VSEDRKLEGIIPRMSVGRNIAVGSLDALSNRFGVIDKKRETQMGVDGKREFNIKTPDL
                     EKPIVELSGGNQQKAIVARWLLTKPRVLILDEPTKGIDVGAKSEFYNMICKFAKEGLA
                     VILISSEMPEVIGLSDRIIVMRGRRIAGELPAAEATEEKLLTMAMMEEHVHE"
     gene            complement(5082..6002)
                     /locus_tag="ACZPFK_02260"
     CDS             complement(5082..6002)
                     /locus_tag="ACZPFK_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1405166.1"
                     /translation="MGIYPILLCNQAYHTFDADYSTVCSDVINSMRRLVELLRGRGHG
                     RIALYGVNPQSVSDESRMDSYLAALGGEDGRRHVFFNSGSLENCFEQFMARAQQYDAV
                     ICANDFAAISLVRRLGLRDPALLERLAIAGCADARLTQLYGSHILSVRVNFVEYGRAA
                     VTLLENLRKNPYLSHIIMMIRWDFSLLDGAAAPAPAAHRREKAPALPADPDVFYEDRE
                     LNDMMLLEKLLDGCEDPDYDIIRLLLRGEPYEKIAETCFLTESAIKYRVKKMVRVCCV
                     QGRKELTDLLRRYLPGGDPGGPPGVRPGMR"
BASE COUNT         1189 a         2016 c         2041 g         1258 t
ORIGIN      
        1 ggcgcgcagg tccagcaccg tcctcacata gccctccagc tggtccatcc gcatgcggaa
       61 gccggagaca tacggcgcgg gaaacgggta gggcgtgtga tcggaaaagc ccagcacccg
      121 gcagccgccc tgcagcgcgc gctccacgta ctcccgctcg gcgccggcgg cgtgcaggca
      181 gcgggcggtg tgagtgtgat agttggcgat catctgcccc tctccggcgg ctggggcagc
      241 tccgcatagg ccgccagcac ctgggccgag atgcgctcga tgccgttctc cacatagcgg
      301 cgccccagca gcgcggtggc gtcccgcggg tcctgctcca ccgtgtgggc ggcgttgccg
      361 cggccctgaa attccagcgc gtcggcgatg ccccactcgc aggttttcag cggcacctcc
      421 ctgggcggca gcttgtccag atcaacgctg tcgccgctga tgtacatctg catggaggtc
      481 tcggagatat tggcgtgccc cagacgctgg tcgtcctcct ccagcttttg catggcccgg
      541 gtgaagagca cggtggcggg ggtggtgtgg gtgaattcct ttgccagccg ctcgccggcc
      601 gccagctgcc ccgaggcgcc gtggccgttg accaccacca ccagccggaa gcgctgctcc
      661 acgatgcgca gcagatactc ccgcaggatc atgccgaaag cctcctccgg gaaatagcag
      721 ctcttcaccg aggtcgcggg cacatccatg cccaccacat acaaatcatc gtcctcaaag
      781 cccatgcacc gcagctcctg cggcgtgcgg gcgcgctcgg taccgcaata cagggtgggg
      841 aacaccacgc ccccgatctt ttccgccacc ccgcgcgcca cggcctgcgc gtccaggggg
      901 tcggtgccaa agggcatgtg cgggccgtgc cattccagcg ggcccaccgg caggtaggcc
      961 accgaaaagc gctccttttc cgccaaaagc tcgcccggcc tcatcaattc gtactgaaca
     1021 gtcctcactg tgttacctcc atccccggcg cggccgggtg ttactctctg ggaaaaaaga
     1081 acaatcggga gcttgctccc gattgttcca ctgcgcgatt gaaacttaca gcacgtaatc
     1141 cgtgtagttt tcgggagtga cgatgacggc cggggtcgca tactccgcgg ggatctcggt
     1201 gccgttgacc aggtactcga cgacggcggt gatcgcctct ttgccgacca ccttgccgga
     1261 gaagtaggcg gaggcgcggc agacggagta gttgccgtcg gcccactggt tgctcatctc
     1321 gtcgcagccc agggcgatga cgcagctggt ggcgtccagg cccgcctctt ccagcacggc
     1381 gccgcagccc agcacgccgt tctcagacgg agtcatgacc aaccaggtct tgatctcggg
     1441 gtgcgcggtg acgaccgcgg aggcgctgtt gtaggcttcc tcggacatgc tcacgtaatc
     1501 ggcctcatag gtgcggtcgc tcagggcgtc gcccagctta tccttccagg cgtctttctc
     1561 gccctcggtg cggggcacgc aggaggagac ggtctccatg gtcatgtaca gcaggcccac
     1621 gctggggtcg tccagcaggt cgttcttctt ggcatagtcg gccatccact cgccggcggt
     1681 gtagccgatg ttgtaggcgt cgatgccgaa ccagggggcg atcttgttgc cgtcggtgtc
     1741 gatcaggccg tcgtccacag cgatgatggg cacgcccgcg tctttgcact tgtccactac
     1801 cgactgggac atggtctggt cggggatgca ggtgacgatg gcgtcggcgt tgtcggcgat
     1861 ggcggtgtcg atcagggtca tgtacttctc ggggnnnnnn nnnntcgcag ttgttgaaat
     1921 gccaggtggc ctcaaagccg tacttctcgc ccagctcgcc cagggtcttc tcgatcgagg
     1981 cggcctcgtt gacaaaatag gtggactcgg atttgtagat gccgtagatg ttgagggcct
     2041 ccaccttgcc gcctgcagcg gtggagctgg catcgggggt ggagctgaca ggggcggcgg
     2101 acgaagagct gtcggagccg ccgcaggcca ccaggctgag cgccatcatg cctgccagga
     2161 gcagtgatac aaactttttc atgctttttt cctccgtgac ttttttattt tcagccgccg
     2221 ccgcagggag ttctgcggcg cgcggtcaaa aaacgccgtt ttcaggccgc tgtgcacagg
     2281 ccggggcgaa aaggctgccc tttaccggcc cggccagacc gggccctgag cgggaaacaa
     2341 tgccgtgaac gccgcgcgcc gcttttacag cgctttcatg ctgtcggcca gcaggcggcg
     2401 ggcgcgggag cgctggcggt agaagtcgat ggccagcgag gcgaacagca gcatgccgcg
     2461 ggccacgtac tgccagtagg acgagacgcc caccatgttg aggccggagt tgaacgcctg
     2521 caccagcagc acgcccagca ccacgctggg gatagagccg acgccgccca ccatcgacac
     2581 gccgcccagg ttggccgcgg tgatggcgtc aaagtgcagg ttggggctgg ccgcgggttg
     2641 gcccgagttc atgcgggcgg ccaggatgat gcccgccagg gccgcgaaca tggaggtcat
     2701 cacatagcag atggtcttga cgcggttggc gttgatgccc gccaggcggg cggcctcggc
     2761 gttgccgccg atggcaaaaa tgctgcggcc aaacttggtc ttggccagga tataggcgaa
     2821 gatgatgaag aagatcacca tcaggatgac cgtgtagggg atgccgtcgt cgccgcccag
     2881 ccgggatttg ccaaaggcca taaagccctt gtccgcgacc gagaccggct tgccgccgca
     2941 cacgagatag gcgaagccgc gccagaccga ctggctcgcc aacgtggcga taaagggcac
     3001 gatgttgagc ttggtgacca tcagcgcgtt cagcaggccc gccaccgcgg caatggcgac
     3061 ggcggccagg cagagcagcg gccagggcag gctggtggct ttcagcgccc aggcgacaaa
     3121 gacgccggca aaggcggcca ccgcgccggc ggacaggtcg ttgccgccgg tcatgatcaa
     3181 aaaggtgatg ccgatcgagg tcagcccgat cagggtggaa gagaccagga tgttgataaa
     3241 attagtgtag gtaaaatagt tggggttgag caccgagaag ataacggtga ccccgaccag
     3301 ggcgcagatc agcaccagct ggttgccgga gatgggcagc ttgaactttt tctcattcat
     3361 gaacgtgttc ctccatcatt gccatggtga gcagcttttc ctcggtcgcc tcggcggcgg
     3421 gcagctcacc tgcgatccgc ctgccccgca tgacgatgat gcggtccgac agcccgatga
     3481 cctcgggcat ctcagaggag atgaggatca ccgccagccc ctccttggcg aacttgcaga
     3541 tcatgttgta gaactcggac ttggcgccca cgtcgatgcc cttggtgggc tcgtccagga
     3601 tcagcacccg cggctttgtg agcagccagc gggccacgat cgccttttgc tgattgccgc
     3661 cggacagctc cacaatcggt ttttccaggt ccggggtctt gatattgaac tcccgcttgc
     3721 cgtcgacgcc catctgtgtc tcgcgctttt tgtcgatgac gccgaagcgg ttggacagcg
     3781 cgtccaggct gcccacggcg atgttgcgcc ccacgctcat gcgcgggatg atgccctcca
     3841 gcttgcggtc ctcggacacc atcaccacgc cgtttttgat ggcgtcgtgg ggggatttgc
     3901 agaccagggg cttgccgtcc agcagcacct cgcccccggt gcgcgggtcc gcgccggtga
     3961 tggcccgcac cacctcggtg cgccctgcgc ccaccaggcc ggaaaaacca accacctcgc
     4021 ctgcgcgcac cgtgaaagag gcgtctttca ggtaggggga ctgcaggttt ttcacctcca
     4081 gcaccacctc gccgatctct ttatccttgt ccagggcggc aaagacgtcg cccaggtcgc
     4141 gccccaccat cttttcgatc agctgcttgt cggtgatctc ctggatgggc gcctcgccca
     4201 caaagcagcc gtccttgaag atggccaccc ggtcggcgac ctcgcggatc tccgccatgc
     4261 ggtgggagac gtagatgatc accttgccct cgtcccgcag cttgcggatg atgcggaaca
     4321 ggctgtcgat ctcggcgtcg ctcaggctgg ccgtgggctc gtcgaaagcg atcatcttca
     4381 ggttttccct gctgtaggct ttcatgatct ccaccatctg ctgataggcc acgctcaggt
     4441 ctttcacctt gtcggtggag cgcatgtcca gcccaaactc gtcgatgatc ttctgcgccc
     4501 gctcatgcac ctcattcatc ttgatgtggc ccaaaaagcc ggtgggcagc cgtcccagaa
     4561 aaatgttttc gcccacagtc agctccagca tgatctggcg ctcctgatag atgacgccga
     4621 tgcctgcggc caccgcctcg cgggggctgc tgaagtggca gggctcgccg ttgaccagat
     4681 agtcgcccat gtcctgctgg taatcgccgt tgaggacctt gagcagcgtg gatttgcccg
     4741 cgccgttctc tcccatcagg gccaacacct ctccgcccct ggcggtgaag ctgatgctgt
     4801 ccagggcttt cacgccggga aagtactttg tgatgttcct gaactcgata acgtttccca
     4861 tttccttctc ccttatcact tttacctgcg gccataccgg ctggaacttg aattttgttg
     4921 cgaatctctg ctttgatttt atgataaatg attataatac ttttcaaaag tctatttttt
     4981 atgttaaaaa taacgtattt tttagacgat gacaaaaaga ggcgctgtcc gccccctagg
     5041 cccggggccg gcagcgcctc tttttgttga aaaggcagga atcaccgcat gccgggtcga
     5101 acgcccggcg ggccgcccgg gtcgcccccg ggcagatagc gccgcagcag gtcggtgagc
     5161 tctttgcggc cctgcacgca gcagacgcgc accatctttt tcacgcggta cttgatggcg
     5221 ctctcggtca aaaagcaggt ctcggcgatc ttttcatagg gctccccccg cagcagcagg
     5281 cggatgatgt cgtagtccgg gtcctcgcat ccatccagca gcttctccag cagcatcata
     5341 tcgttgagct cccggtcctc ataaaagaca tccgggtcgg cgggcagggc gggggctttt
     5401 tcccgccgat gcgccgccgg ggcgggcgcc gcggccccgt ccaacagact gaaatcccag
     5461 cggatcatca tgatgatgtg ggacagatag gggtttttgc gcagattttc cagcagggtc
     5521 accgcggccc ggccgtactc gacaaagttc acccgcaccg acaggatatg gctgccgtac
     5581 agctgggtaa gccgcgcgtc ggcgcagccg gcgatggcca gcctttccag cagggccggg
     5641 tcccgcagcc ccagccgccg caccagcgag atggccgcaa aatcgttggc gcagatcacg
     5701 gcgtcatact gctgtgcgcg ggccataaac tgctcaaagc agttttcgag cgaaccgctg
     5761 ttgaaaaaca cgtgccgccg cccatcctcc ccgcccaggg cggccagata gctgtccatg
     5821 cggctctcgt ccgagacgga ctgcgggttg acgccgtaca gggcgatgcg gccgtggccc
     5881 cggccgcgca gcagctccac caggcggcgc atggagttga tcacgtcgct gcagacggtg
     5941 ctgtaatccg cgtcgaatgt gtggtaggcc tggttgcaca gcaggatggg atagatcccc
     6001 atccggttgc agcccggcag ggccgccgcc gacgatgatc agatcataaa tattgtccat
     6061 acaggtcctt cccttttctg gattcttatt tcattctatt tttgttttcg aaaaaaaact
     6121 gttgccgcgc caacaatttt gcaaaatagt tttcatggat gaaagaaaac gaggcggagg
     6181 cgggcggccc ggacaggccc tgtgcgggag aaaatgcaag gaggcggcgg ggagactccg
     6241 ctgtcatcct atgcccgccg cccgcgccgc atgcgcgtct agggaacccg tgcaggcgga
     6301 aagccgcgcc gggaaagggg accgtctgct gggtagcggc gatattgacg gcggctgttg
     6361 tcggggagct gcaggggttg cggggggtgt gggagaaaca gagggagagg accacagaaa
     6421 acgaaaaaag tctcaaaatg atttaatatt ataaagtaaa aagaaattat gcgacatctg
     6481 cggcttgtaa tggcggggaa caaagtgcta taat
//
LOCUS       DBMDYA010000054         6510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3019_length_6510_cov_1.677641, whole genome shotgun sequence.
ACCESSION   DBMDYA010000054 DBMDYA010000000
VERSION     DBMDYA010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3019_length_6510_cov_1.677641"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            309..776
                     /locus_tag="ACZPFK_02265"
     CDS             309..776
                     /locus_tag="ACZPFK_02265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1405167.1"
                     /translation="MRREDREVKEFDAILKIVAACKVCRLALWDEEGPYIVPLNFGYE
                     VKGGRLTLYFHSAKEGRKLRALAAEPRAAFEMDGSHRLIEAERACGYGYAFESVVGSG
                     RAALVSDPEEKRRALALLMRHQTGREFSFGDAEAAAVAVIRLEVERLSGKLHP"
     gene            complement(925..2046)
                     /locus_tag="ACZPFK_02270"
     CDS             complement(925..2046)
                     /locus_tag="ACZPFK_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HIR1405168.1"
                     /translation="MKYCKKCTMPDTRPGITFDAEGVCSACRHYEHRKDVDWDARWKE
                     FEAMCDKYRGCNGPGGYDCAVAVSGGKDSHFQVYILKEVMHMNPILFSVEDNFPMTEA
                     GKHNLKNISEAFGCTIISCKPNIQAQKKLMRVFFEKYGKPTWYVDRLIYTFPLHMAAK
                     FNTPFLCYGENVSFEYGGSSDEETYSAKGQIENGVAVGFPKEELLSYGVSENDLILTE
                     APSAEELARLDPFYMSYFLPWNSLKNYQVAKEHGFHDLTHEWDRTHHAENFDQIDSRA
                     YLVHSWLKYPKFGHAAATDYTARYIRYGLMTREEAIPVIKQRDGALDPLCVRDFCEFC
                     GYSESEFWAILDKFYNTDLFRKDEYGRWVLKEPIWEEEN"
     gene            complement(2163..2480)
                     /locus_tag="ACZPFK_02275"
     CDS             complement(2163..2480)
                     /locus_tag="ACZPFK_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405169.1"
                     /translation="MTKGMDTAFIQQRITELRLQKNVSEYKMSLDLGQSRSYIQGISS
                     GKVLPSMAMFLEICDYLDVTPAEFFSPGRAEAVLTQKVVQKVRELPPRTLALLDEWLG
                     LLK"
     gene            2626..2844
                     /locus_tag="ACZPFK_02280"
     CDS             2626..2844
                     /locus_tag="ACZPFK_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405170.1"
                     /translation="MLLETLRCRVGCLYLSDLKTEPYNRRARLEAQLLRAEDFTLIEW
                     NDAVEYLMGRGAGGHTCARAAQRALVWK"
     gene            complement(2928..4244)
                     /locus_tag="ACZPFK_02285"
     CDS             complement(2928..4244)
                     /locus_tag="ACZPFK_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface carbohydrate biosynthesis protein"
                     /protein_id="HIR1405171.1"
                     /translation="MALDFLILYEHVVREYESLLLLQAELERRGYSVEIRQLLDRKKL
                     KYFTWKRPRVLVASNLYDDEGLNSHVYNNVGCCERVVNLHWEQMLSDTQEAEPWFNFS
                     GNARKCVQTCWGERTRQRLIEHGVPPRNAVVTGAVMMDFLRPEFEGYLMDRASLCREF
                     GLDPGKRLLLYISSFGYASMGEAEVAELSRMAGQDFSEFARVNRESMAATLEWFDQYL
                     AAHPEVELVYRRHPSEWDSPALAALAKKRSNFHVIFEHSVKQWIVAADDIFIWMSTAI
                     AEVYFAGKSCRILRPQPIPHEFDPVIYRDGDYITDRAAFERALASPQAAFPLPRQVIE
                     GYFDSDPARPAYLRMADLLEQVHREPPRDHPFVKAFRPHFNWLKCFALIGVHVLDALH
                     LDPARFRRVCPPFADFAGRIYGYIQKAKVDPAQVRAWRQKIARYVK"
     gene            complement(4244..5269)
                     /locus_tag="ACZPFK_02290"
     CDS             complement(4244..5269)
                     /locus_tag="ACZPFK_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405172.1"
                     /translation="MKKFIAKLALALLPLALYLAVFIAFEPNNYFGLRGVTNGNAPIA
                     RLRAYELDPQPNLLLGDSRMAHFDMALVDEVSGLSWANVSYGGASLEESIDEFYYLYE
                     NHPDIRRVVLGVSFYTLNAAYRTANRMATIETQLKNPAAYIFNLEYNVNTLTVIGDKL
                     AGRPDVEETAEHTAEEYEENGAPLPYRRDLIDYAATLYGNCAVGGALPQRVYAEDGAL
                     ANAAEMAAAMLAATPAQSRFSVNEAALEKLLALAEFCRQNGVELTVVLPPMDESVRRL
                     VCEPLGIDEAMRPALAALAASGARVLDYEWQGDPAYADTMFYDGFHIDTVHGLPQWTR
                     TLFGEVA"
     gene            complement(5281..>6510)
                     /locus_tag="ACZPFK_02295"
     CDS             complement(5281..>6510)
                     /locus_tag="ACZPFK_02295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1405173.1"
                     /translation="VPTLLGAAGGGLVRLAFPLGISFYTFATISYLVDVARGDMEPET
                     DFVAYAAFVTFFGTITSGPICRAQKLLPQLRRPRRWDAQRGADALRLILFGLFKWVAV
                     ANVLGLYVNQVFENAAGYQGLTLIFAALLYALQLYFEFAGYSDVARGTALLLGLEIPV
                     NFKTPYFSTNFSAFWNRWHISLSSWLQDYIFLPLVWGRWTSRLPLVGKRVENPPMLSS
                     LVILFFVSGFWHGNSLPFVVWGLLQALYRVGEELLHRFYKKPKKRPGLPLRVFKTAGV
                     FCLWTASLVFFRIGLLPQGTVGDALSYLARQFRGWSPAAFLADTSAAVQAGFYAKPIM
                     VAAYFAFLAAVLAAAFFADWRQCFRYKDQHISLAVARLRPAARWAVYYLLIGCILVGT
                     ILQSGGFGTVSFAYAGF"
BASE COUNT         1196 a         1986 c         2159 g         1169 t
ORIGIN      
        1 agaaatagcc gtaaggctat tttttcggcc atccgacccg tcctcgggca cggccctgga
       61 ttgcggagca aaaacaaata gccgcacagc tatctgttga gccatgcgtt ccggctgccg
      121 cgcggcggcg ggcaacgggg ccgggggaga ggcgcgagcc tgcttccgca ctgcgaactt
      181 tttggcggct gcttgcggcg cgggcggccg cgcgcaaaaa tcagcggcac gggcaaagcc
      241 ttttgcgccg cacctgtaaa aaacagccgg gccgctctgc ggcagcggcg ggaaagggga
      301 gagcgcgcat gcgcagagag gaccgggagg taaaggagtt tgacgccatt ttaaagatcg
      361 tggcggcctg caaggtctgc aggctggcgc tgtgggacga ggagggcccc tacatcgtgc
      421 cgctgaactt cggctatgag gtgaaagggg ggcggctgac cctctatttc cacagcgcca
      481 aggagggccg caagctgcgg gcgctggcgg cggagccccg ggcggccttt gagatggacg
      541 gcagccatcg gctgatcgag gcggagcggg cctgcggcta cggctacgct tttgagagcg
      601 tcgtgggcag cgggcgggcg gccctggtgt cggacccgga ggagaagcgc cgcgccctgg
      661 cgctgctgat gcgtcaccag acgggccggg agtttagctt cggcgacgcc gaggcggcgg
      721 cggtggcggt catccggctg gaggtggagc gcctttcggg caagctccac ccctgaaccg
      781 ggcggcggcc cgtaccgccc tggaaaaaag agaaaagcgc gccccgcggc cggtttccaa
      841 gccggccgcg gggcgcgctt tttgatttta tccgtctgtc tgcccggtat tcacggcttt
      901 ccgcgcgggg ccggggcaag ggtcttagtt ctcttcttcc cagatcggct ctttcagcac
      961 ccagcggccg tactcgtcct tgcggaagag gtcggtgttg tagaacttgt ccaggatggc
     1021 ccagaactcg ctctcgctgt agccgcagaa ctcgcagaaa tcccgcacgc agagcgggtc
     1081 cagcgcgccg tcccgctgct tgatgacggg gatggcctcc tcgcgggtca tcaggccgta
     1141 gcggatgtag cgggcggtgt agtcggtggc cgccgcgtgg ccgaatttgg ggtatttcag
     1201 ccagctgtgc accagatagg cgcggctgtc gatctgatcg aagttctcgg cgtgatgggt
     1261 gcggtcccac tcgtgggtca ggtcgtggaa gccgtgctcc ttggcgacct gatagttttt
     1321 caggctgttc cagggcagga agtaactcat atagaaagga tccagccggg ccagctcctc
     1381 cgcagagggt gcctcggtga ggatgaggtc gttctcgctg acgccatagc tgagcagctc
     1441 ctctttgggg aagcccaccg ccacgccgtt ctcgatctgc cccttggccg aataggtctc
     1501 ctcgtccgag ctgccgccgt actcaaagga gacgttctcg ccgtagcaga gaaagggggt
     1561 gttgaacttg gccgccatgt gcagcgggaa agtgtagatc agccggtcca cataccaggt
     1621 gggcttgccg tatttttcaa agaacacccg catcagtttt ttctgcgcct ggatattggg
     1681 cttgcagctg atgatggtgc agccaaacgc ctcgctgatg tttttcaggt tgtgcttgcc
     1741 cgcctcggtc atggggaaat tgtcctccac gctgaagagg atcgggttca tgtgcatgac
     1801 ctctttgagg atatacacct gaaaatggct gtccttgccg cccgagacgg ccaccgcgca
     1861 gtcgtagccg ccggggccgt tgcagccgcg gtacttgtcg cacatggctt caaactcttt
     1921 ccagcgggcg tcccagtcaa cgtccttgcg gtgctcgtag tggcggcagg cggagcagac
     1981 accctcggcg tcgaacgtga tgccgggccg ggtgtccggc atggtgcatt ttttgcagta
     2041 tttcatctgg tttctcctta ctgtttactg tctcgatcag acgttttgca tccggggcag
     2101 cgcgcgcggc ggcccgtctt tattgtaccc cattttagag gctgctacaa ggcggcaggc
     2161 cgctatttca gcagccccag ccactcgtcc agcagggcca gggtgcgggg cggcagttcg
     2221 cgcactttct gcaccacctt ttgggtcagc accgcctccg cccggcccgg agaaaaaaat
     2281 tccgcaggcg tgacgtccag gtagtcgcag atctccagaa acatggccat cgagggcagc
     2341 accttgccgg aggagatgcc ctggatatag ctgcggctct ggcccaggtc caggctcatc
     2401 ttatactccg acacgttttt ttgcagccgc agctcggtga tgcgctgctg gataaaagcg
     2461 gtatccatgc ctttggtcac acctttccca gctattatga taccgcttta aaattgtctt
     2521 attaattttt caaatcgcga atatttaaag ttttgattga tattacaatt tttagaagtt
     2581 ataataaaaa tataaaacat ttttcagaag tataaggagg gccatatgct gctggagacg
     2641 ctgcggtgcc gggtggggtg cctctatctg tccgatctga aaacggagcc ttataaccgt
     2701 cgggcccgcc tggaggccca actgctgcgg gctgaggact tcacgctcat agagtggaac
     2761 gacgccgtag agtatctgat ggggcggggg gccggcggtc acacctgtgc gcgtgcggcg
     2821 cagcgcgccc tggtctggaa atagcttttt cggcagagaa aaacagtgcg gcgcaccggg
     2881 ggggccggtg cgccgcactg tttttgtttt caattgtttt ctgcgactca tttcacatag
     2941 cgggcgatct tctgccgcca ggcccgcacc tgggccgggt ccacctttgc cttttggata
     3001 tagccgtaga tgcggccggc aaagtcggcg aaaggcgggc agacgcgccg gaagcgggcg
     3061 gggtccaggt gcagggcgtc caggacgtgc acgccgatga gggcgaagca cttcagccag
     3121 ttgaagtggg gccggaacgc cttgacgaac gggtggtcgc ggggcggctc gcggtgcacc
     3181 tgctccagca ggtccgccat ccgcaggtag gcgggccggg ccgggtcgct gtcaaagtag
     3241 ccctcgatca cctgccgcgg gaggggaaaa gccgcctgcg gcgaggccag ggcccgctca
     3301 aacgccgcgc ggtcggtaat gtagtcgccg tcccggtaga tgaccgggtc gaactcgtgg
     3361 gggatgggct gcgggcgcag gatgcggcag ctcttgccgg cgaaatagac ctccgcaatg
     3421 gccgtgctca tccagatgaa gatgtcgtcg gcggcaacga tccactgctt caccgagtgc
     3481 tcgaagatga cgtggaagtt ggatcgcttt ttcgccaggg ccgccagggc cgggctgtcc
     3541 cactcggagg ggtggcggcg gtagaccagc tccacctccg ggtgggcggc gagatactgg
     3601 tcgaaccact ccagcgtggc ggccatcgac tcccggttga cgcgggcgaa ttcactgaaa
     3661 tcctgccccg ccatgcggga cagctccgcc acctcggcct cgcccatcga ggcgtagccg
     3721 aagctggaga tgtacagcag caaccgcttg cccgggtcaa ggccgaactc ccggcagagg
     3781 gaggcgcggt ccatcaaata gccctcgaac tccggccgca gaaagtccat catcaccgcg
     3841 ccggtcacca ccgcgttccg gggcggcacc ccgtgctcga tcagccgctg ccgcgtgcgc
     3901 tcgccccagc aggtctgcac gcatttgcgc gcattgccgg aaaaattgaa ccagggctcc
     3961 gcctcctggg tgtccgagag catctgctcc cagtgcaggt tcaccacccg ctcgcagcag
     4021 cccacgttgt tgtagacgtg gctgttgagc ccctcgtcgt cgtaaaggtt gctggccacc
     4081 agcacccgcg ggcgcttcca ggtgaaatac ttcagctttt tgcggtccag cagctgacgg
     4141 atctccaccg agtagccccg ccgctccagc tcggcctgca gcagcagcag gctctcatat
     4201 tcccgcacca catgctcgta gaggatgaga aaatccagcg ccatcaggcc acctccccaa
     4261 acagcgtccg ggtccactgg ggcagcccgt gcaccgtgtc gatgtgaaag ccgtcgtaga
     4321 acatggtgtc cgcgtaggcg gggtcgccct gccactcgta gtccagcacc cgggcgccgc
     4381 tggccgccag ggccgccagg gcgggccgca tggcctcgtc gatgcccagc ggctcgcaga
     4441 ccagccgccg cacgctctcg tccatggggg gcagcacgac ggtcagctca acgccgttct
     4501 gccggcagaa ctccgccagg gcaagcagct tttccagcgc cgcctcgttc accgagaacc
     4561 ggctctgcgc aggggtggcg gccagcatgg ccgccgccat ctcggcggcg ttggcgaggg
     4621 cgccgtcctc ggcgtagacc cgctgcggca gggccccgcc caccgcgcag ttgccgtaga
     4681 gggtggcggc gtagtcgatc aggtcgcgcc ggtagggcag gggggcgccg ttttcctcgt
     4741 attcctccgc cgtgtgctcg gcggtctcct ccacgtcggg gcggccggcc agcttgtccc
     4801 cgatcacggt gagggtgttg acgttgtatt ctagattaaa aatataggcc gccggattct
     4861 tgagctgggt ctcgatggtg gccatgcggt tggcggtgcg gtaagcggcg ttcagggtgt
     4921 aaaaagagac gcccagcacc acccgccgaa tgtccggatg gttttcgtac aggtagtaaa
     4981 attcgtcgat gctttcctcc aggctggcgc cgccgtagga cacgttggcc caggacaggc
     5041 cggacacctc atccaccagc gccatgtcga aatgggccat gcggctgtcg cccagcagca
     5101 ggttgggctg cgggtccagc tcgtaggcgc gcagccgcgc gatgggggcg ttgccgttgg
     5161 tgacgccccg caggccgaaa tagttgttgg gctcaaaggc gatgaacacc gccaggtaca
     5221 aagccagcgg cagcagggcc agggccagct ttgcgataaa ctttttcatg gccgccctcc
     5281 tcaaaagcct gcgtaggcaa agctcacggt gccaaagccg ccgctctgca gaatggtgcc
     5341 caccagaatg cagccgatga gcagataata gaccgcccag cgcgcggccg ggcgcagcct
     5401 tgccaccgcc agcgagatgt gctggtcctt ataacgaaag cactgccgcc agtcggcaaa
     5461 aaaggcggcg gccagcaccg cggcgagaaa ggcgaaataa gcggccacca tgatgggctt
     5521 ggcgtaaaag cccgcctgca cggcggcgct ggtgtcggcc aggaacgccg ccggcgacca
     5581 gccgcggaac tgccgggcca gataggagag cgcgtccccc acagtgccct ggggcagcag
     5641 cccgatgcgg aagaagacca ggctggccgt ccacaggcag aacacccccg cggtcttgaa
     5701 gacgcgcagc ggcagcccgg ggcgcttttt gggctttttg taaaagcggt gcagcagctc
     5761 ttcccccacc cggtagaggg cctgcagcag gccccacacc acgaatggca aactgttgcc
     5821 gtgccagaag ccgctgacaa agaacaggat caccaggctg gagagcatgg gcgggttttc
     5881 gacccgcttg cccaccagcg gcaggcggct ggtccaacgg ccccagacca gcggcagaaa
     5941 gatatagtcc tgcagccagc tggacaggga gatgtgccag cggttccaga aagcggagaa
     6001 gttggtggaa aaataggggg tcttgaagtt caccgggatc tcgagcccca gcagcaaggc
     6061 ggtgccccgg gccacgtcgg agtagcccgc aaactcaaag tagagctgca gcgcatagag
     6121 cagggccgca aagatcaggg ttaacccctg gtagccggcc gcgttctcga agacctggtt
     6181 gacgtaaagg cccagcacat tggcgacggc cacccatttg aagagcccga acaggatcag
     6241 gcgcagcgcg tctgcgccgc gctgggcgtc ccagcggcgg ggccggcgca gctggggcag
     6301 cagcttctgc gcccggcaga tggggccgct ggtgatggtg ccgaaaaagg tgacgaaagc
     6361 cgcgtaggcg acaaagtcgg tctccggctc catgtcgccc cgggccacat ccaccaggta
     6421 gctgatggtg gcaaaggtgt aaaagctgat ccccagcggg aaggccagcc tgacaagacc
     6481 gcccccggcg gcgcccagca gggtgggcac
//
LOCUS       DBMDYA010000055         6503 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3024_length_6503_cov_1.560294, whole genome shotgun sequence.
ACCESSION   DBMDYA010000055 DBMDYA010000000
VERSION     DBMDYA010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_6503_cov_1.560294"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="ACZPFK_02300"
     CDS             <1..541
                     /locus_tag="ACZPFK_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1405174.1"
                     /translation="EQVMVMGTGTNGMLLAQLIRHSNAAEVVALGGNPDKLALLNAAG
                     VPTIRMDRNDYTVHERAVLERFPNKLDAIVDATASGPLQEHGMTLLKKGGRMLVYSVA
                     HAGGEVRLDRRMMYSNELTYYYTMHQTGNFDRALRAIETGAVRLDGMVSAEYRLDDYF
                     EAIRAVKTDPSLLKVIIHP"
     gene            556..1611
                     /locus_tag="ACZPFK_02305"
     CDS             556..1611
                     /locus_tag="ACZPFK_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR1405175.1"
                     /translation="MKKNRAVYMNGKLHFEYGELELPRAAPGEVVVRVEYVGICGSDV
                     HFYQTGMIGPRPIVGRQILGHECAGTVAEVGAGVTALSVGDRVALEPGEPCGSCEYCK
                     QGRYNLCPQVKFLSSNPYQGAMRDYMSYPAHLAFKLPDNVSTLEGALVEPLAVGLHAA
                     RQGGVQLGDTAVILGAGCIGLTTLLACKAMGASRVIVTDIFEKRLEVARRLGADEVVD
                     GSQVDSTAEVLRLTGGAGGEVVFETAGSRVTAAQTEQVAKRGGTIVMVGNIMGETPFD
                     FHRVSTQEITIKSVFRYRNIYPLAIQGIASGQIDVKSIVSHIFSFDQVPEAFDKAAND
                     KLNVVKAVIRVKEEAEK"
     gene            1608..2078
                     /locus_tag="ACZPFK_02310"
     CDS             1608..2078
                     /locus_tag="ACZPFK_02310"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR1405176.1"
                     /translation="MKIAMGCAKVAYDLKEAVKAHLLGRGFEVVDFCEDPADPMPYAE
                     VAARAAQGMLDAGCERGILFCGTGMGVSLVANLFPGITAAVVESKFAARRCRYTNNAN
                     VLCMGSFLLGPVLACEMADEFLDTEWLQGADEGAREVLGQQMAFIRELEQKYRR"
     gene            2111..3283
                     /locus_tag="ACZPFK_02315"
     CDS             2111..3283
                     /locus_tag="ACZPFK_02315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1405177.1"
                     /translation="MEDFDFCLDTRFVFGKGVEARAGEFCKKYARRVLLLDYGGGTGE
                     QQRLHETVKDSLQRVGLTVFEMDGVVPNPLYSFAQKGIEVCRENGVELVLAVGGGSVI
                     DTAKCIAVGAVYEGDVWRDFYVGRKYPEKWLPLGAIPTIAAAGSEGSVASILVEDATG
                     QKYSFKPCPRPTFALINPELTYSVPAYHTACGAVDMMSHALERYFTNTPQCDLTDGLI
                     EGLLRAVIQNAPVLLERPDCYEARAELSWAAVLAQCDLLDTGRMADWSCHNIEHEVAH
                     YCHSAHGAGLAVVTPAWMRYVYRHDLPRFVRYAVRVWEVADDPADPEGVALEGIRRTR
                     AFFERLGMPVSLADLGAGQPDCRGMARDCIQLYGQLGKFVPLGEEDVYRILLSAAR"
     gene            3308..4168
                     /locus_tag="ACZPFK_02320"
     CDS             3308..4168
                     /locus_tag="ACZPFK_02320"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1405178.1"
                     /translation="MLTKEREKELRKLAVDIRGQIARCIAHTEAHSGHVGGSFSLAEL
                     MAVIYGEVMRVDPQNPKWKDRDYFVLSKGHCGPALYATLALRGFFPMELLDTMNRGGT
                     DLPSHSDRLHTPGVDMSTGSLGQGASTAVGLAYADKLDGRGDRYTYLVLGDGEITEGQ
                     VWEAANFAAAHGLGNLIAFVDKNGKMDPFSPYTADPENISQRFAAFGWDAQDCDGHNV
                     RAVYDCIQRAKGAPGPSVIVLHTHKGKSIPEIEEMETNHSIPVGDELLEKLTQAFERQ
                     KEEIDREGGM"
     gene            4170..5135
                     /locus_tag="ACZPFK_02325"
     CDS             4170..5135
                     /locus_tag="ACZPFK_02325"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1405179.1"
                     /translation="MAVKLLAERKEGALTPVLAQTLDEMFACDERLVYMDCALARASG
                     VTALFGKYGDRAVDCGIQEQNMLGAAAGMSIAGKVPFVHTFSIFIARRACDQLFLSGC
                     YNRANVKVLASDPGVSATTNGGTHMPFEDVSIVRAFPEMTLLDCADAVMLKSLLRTMA
                     DTYGMMYLRFPRKATVNVYDEGSEFTIGKANLLREGTDVTIIACGMEVAEALQAAEEL
                     AAEGVSARVVDMFTIKPLDAEMVLESARRTGAVVTAENANYLGGLGSAVAETLGEHLP
                     TPMERIGVKDLFGEVGTLDYLKKRFELTSVDIAAACRRVIARKGG"
     gene            5143..6333
                     /locus_tag="ACZPFK_02330"
     CDS             5143..6333
                     /locus_tag="ACZPFK_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405180.1"
                     /translation="MGQGAKREIRTFKLFTVLFWFSNYIVTPYITPYLQSLGAGNIVI
                     GYVVSSYSIAQILVRVPLGIASDRMGRRKSFIVAGAVLTLVSVTGLWLTRSVAWVFVF
                     RTLQGLAVTSWVCATVLFAGYLRPEEAVGATVQLQVCNSGGTLVGYLLGGLVAKRFGY
                     GSVFLVAASGAALALAVSARIAEAPPGERPLGTKALLRVGASPFLLFVAGMAVFGQII
                     LSATTWGFTPQIATSLGADATGIAACSMCFSVAGLLAALLVTRRLSGWLGEKRTLVLA
                     MALEAVFCIAQPYNPALGTLYVNCFLNGLVHNTSYSLLLGLAIELFPERMRAAAMGWH
                     QAIYALGLLIGPVVAGYFSELYDLKTAFLVIGLLGLAGAVLAQLFYARARGATLRQME
                     KETR"
BASE COUNT         1149 a         1837 c         2369 g         1148 t
ORIGIN      
        1 ggaacaggtt atggtgatgg gcaccggcac caacggcatg ctgttggccc aactcatccg
       61 ccattccaac gccgccgagg tggtggcgct gggcggcaac ccggacaagc tggcgctgct
      121 gaacgccgcc ggggtgccca ccatccgcat ggaccgcaat gattacacgg tccacgagcg
      181 ggcggtgctg gagcgtttcc ccaacaagct ggacgccatt gtggacgcca ctgcctccgg
      241 cccgctgcag gagcatggga tgaccctgct gaaaaagggc ggccggatgc tggtttactc
      301 ggtggcccac gccggcggcg aggtgcgcct tgaccgcagg atgatgtact caaacgagct
      361 cacctattat tacaccatgc accagacggg aaattttgac cgggccctgc gcgccattga
      421 gaccggcgcg gtgcggctgg acggcatggt cagcgccgag taccggctgg atgactactt
      481 tgaggcgatc cgggctgtca agaccgaccc ctcgctgctg aaggtcatca tccatcccta
      541 ggggggagaa caaagatgaa aaagaacaga gcggtctata tgaacggcaa gctgcatttt
      601 gagtacggcg agctggagct gcccagagcc gccccgggag aggtggtggt gcgggtcgaa
      661 tacgtgggca tctgcggctc ggacgtgcac ttttaccaga ccggcatgat cggcccgcgg
      721 cccatcgtgg ggcggcagat cctgggccac gagtgtgcgg gcacggtggc ggaggtgggc
      781 gcgggggtga cggcgctgtc ggtgggggac cgggtggcgc tggagccggg cgagccctgc
      841 ggctcctgcg agtactgcaa gcaggggcgc tacaacctgt gcccgcaggt gaaattcctc
      901 tcctccaacc cctatcaggg ggcgatgcgg gactatatga gctatccggc ccacctggct
      961 ttcaagctgc cggacaacgt ctccactttg gagggggcgc tggtggagcc gctggccgta
     1021 ggcctgcatg cagcgcgcca gggcggcgtg cagctggggg acacggcggt cattttgggc
     1081 gccggctgca tcggcctcac caccctgctg gcctgcaaag ccatgggggc gtcgcgggtc
     1141 atcgtcaccg acatctttga aaaacggctg gaggtggcca ggcgcctggg cgcggacgag
     1201 gtggtggacg gctcgcaggt cgacagcacc gccgaggtgc tgcggctgac cggcggcgca
     1261 ggcggggagg tggtgttcga gacggccggc agccgggtga cggcggccca gaccgagcag
     1321 gtggccaagc ggggcggcac catcgtgatg gtgggcaata tcatgggcga gacccccttt
     1381 gactttcacc gggtctccac ccaggagatc acgatcaagt cggtctttcg ctatcgcaat
     1441 atctatccgc tggccatcca gggcatcgcc agcggccaga tcgacgtgaa atcaatcgtg
     1501 tcgcacatct tttcttttga tcaggtcccg gaggcgtttg acaaagcggc caacgacaag
     1561 ctgaacgtgg tcaaagcggt gatccgggta aaagaggagg cggagaaatg aagatcgcga
     1621 tggggtgcgc caaggtggcg tatgacctga aagaggccgt caaggcccac ctgctagggc
     1681 gggggtttga ggtggtggat ttctgcgagg atccggcgga cccgatgccc tacgccgagg
     1741 tggccgcccg ggccgcccag ggtatgctgg acgccggctg tgagcggggc atcctgttct
     1801 gcggcaccgg catgggggtg agcctggtgg cgaatctgtt cccgggcatc accgcggcgg
     1861 tggtggagag caagttcgcg gcgcggcgct gccgttacac caacaacgcc aacgtgctgt
     1921 gcatggggtc gtttcttttg gggccggtgc tggcctgcga gatggcggac gagtttttgg
     1981 ataccgagtg gctgcagggg gcggatgagg gcgccagaga ggtgctgggc caacagatgg
     2041 cgttcatccg cgagctggag caaaagtacc gtcgctgagg cgggcggcca ggagaggagt
     2101 gacagcgcag atggaagatt tcgatttttg ccttgacacc cgatttgtct tcggcaaggg
     2161 tgtggaggcg cgggcgggcg aattctgcaa aaagtacgcc cgccgtgtgt tgctgctgga
     2221 ttacgggggc ggcaccggcg agcagcaaag gctgcacgag acggtaaaag actcgctgca
     2281 gcgggtcggc ctcacggtgt tcgagatgga cggcgtggtg cccaaccccc tgtattcgtt
     2341 tgcccaaaag ggcatagagg tgtgcaggga gaacggggtg gagctggtgc tggcggtggg
     2401 cggcggcagc gtcatcgaca cggcaaagtg tatcgccgtg ggcgcggtgt atgagggcga
     2461 cgtctggcgg gatttttatg tgggcaggaa ataccccgaa aaatggctgc cgctgggggc
     2521 gatccccacc atcgcggccg cgggcagcga gggctcggtg gcttcgattc tggtggagga
     2581 cgccaccggc caaaagtact ctttcaaacc ctgcccgcgg ccaacgttcg cgctcatcaa
     2641 ccctgagctc acctactcgg tgccggccta tcacaccgcc tgcggggcgg tggatatgat
     2701 gtctcacgca ctggagcggt acttcaccaa cacgcctcag tgcgacttga ccgacggcct
     2761 catagaggga ctgctgcgcg cggtgatcca aaacgcgccg gtgctgttgg agcggccgga
     2821 ttgctatgag gcgcgggcgg agctcagctg ggcggcggtg ctggcccagt gcgatctgct
     2881 ggacaccggg cgcatggcgg actggagctg ccacaacatc gagcacgagg tggcgcacta
     2941 ttgccacagc gcccacggcg ccggtctcgc cgttgtgacc ccggcctgga tgcgctatgt
     3001 ttaccggcac gatctgcccc gctttgttcg ctatgcggtt cgggtatggg aggtggcgga
     3061 cgacccggcg gacccggagg gggtggccct ggagggaatc cggcgcaccc gggcattctt
     3121 tgagcggctg ggcatgccgg tgagcctggc ggacctgggg gcggggcagc ccgactgccg
     3181 cggcatggcg cgggactgca tccagctcta cgggcaactg ggcaaatttg taccgctggg
     3241 cgaggaggac gtgtaccgca tcctgctcag cgcggcgcgc tgacgccggg tggaaaggag
     3301 cagaacgatg ctgacgaaag agagagaaaa agagctgcgg aaattagcgg tggatatccg
     3361 cgggcagatc gcccgctgta tcgcccatac cgaggcccac agcggccatg tgggcgggtc
     3421 tttctcactg gctgagctga tggcggtgat ctacggcgag gtgatgcggg tggacccgca
     3481 gaacccaaag tggaaggacc gggattactt tgtgctgtcc aagggacact gcggcccagc
     3541 gctgtacgca acgctggcgc tgcgcgggtt tttccccatg gagctgctgg acacgatgaa
     3601 ccggggcggc accgacctgc ccagccacag cgaccggctg cacacgcccg gggtggacat
     3661 gtccaccggc tcgctggggc aaggcgcctc cacggcggtg gggctggcct acgcggacaa
     3721 gctggacggg cgcggggaca ggtacaccta tctggtgctg ggggatggcg agatcaccga
     3781 gggccaggtg tgggaggcgg cgaacttcgc cgctgcgcac gggttgggca acctgatcgc
     3841 ctttgtggac aagaacggca agatggatcc tttcagccct tatacggcgg accctgaaaa
     3901 catctcgcag cggtttgcgg ctttcggctg ggacgcacag gattgcgacg gccacaatgt
     3961 gcgcgcggtc tatgactgca tccagcgcgc caaaggcgcg ccgggcccca gtgtgatcgt
     4021 gctgcacacc cataagggca agagcatccc ggagatcgag gagatggaga ccaaccactc
     4081 gattccggtg ggagacgagc tgttggaaaa actgacacag gcatttgagc ggcagaaaga
     4141 ggagatcgac cgggaggggg gaatgtgaga tggcagttaa attgttagcc gagcgcaaag
     4201 agggcgctct gacgccggtg ctggcccaga cgctggacga aatgttcgcc tgcgatgagc
     4261 ggctggtgta tatggactgc gcgctggcca gggcttccgg cgtgacggcg ctcttcggca
     4321 aatatgggga cagggcggtg gactgcggca tccaggagca gaacatgctg ggggcggcgg
     4381 cgggcatgtc cattgcgggc aaggtgccct ttgtgcacac tttcagcatt ttcatcgccc
     4441 gccgcgcctg cgaccagctc tttttatccg gctgctacaa ccgggccaat gtaaaagtgc
     4501 tggccagcga ccccggcgtc tccgccacca ccaacggcgg cacccacatg ccttttgagg
     4561 atgtgagcat cgtgcgggct ttccctgaga tgacgctgct ggactgcgcc gatgccgtga
     4621 tgctgaagag tctgctgcgc accatggcgg acacctacgg catgatgtac ctgcgcttcc
     4681 cgcgcaaggc gacggtgaat gtctacgacg agggcagcga gttcacgatt ggaaaggcga
     4741 acctgctgcg ggagggcacg gatgtgacca tcatcgcctg cggcatggag gtggcggagg
     4801 cgctgcaggc agcggaggag ctggcggcgg aaggggtcag cgcgcgggtg gtggacatgt
     4861 ttaccatcaa gccgctggac gccgaaatgg tgctggagag cgcccgccgg actggcgccg
     4921 tggtcaccgc cgagaacgcc aactatctgg gcggcctggg cagcgccgtg gcggagacgc
     4981 tgggcgagca tctgcccacc cccatggagc ggatcggcgt caaggacctg ttcggcgagg
     5041 tgggcacgct ggactacctg aaaaagcgct ttgagctgac gagcgtggat atcgccgccg
     5101 cctgccgccg cgtgatcgcc cgcaagggcg gctgaggcgc gtatgggaca gggggcaaag
     5161 cgggaaatac gcacgttcaa gctctttacg gtgctgttct ggttttccaa ctatattgta
     5221 acaccctata tcacccccta tctgcaatcg ctgggggcgg gcaacatcgt catcggctat
     5281 gtggtgagca gctacagcat cgcccaaatt ctggtgaggg tgccgctggg catcgcctcc
     5341 gaccggatgg ggcggcgaaa aagcttcatc gtcgccggcg cggtgctgac gctggtgtcg
     5401 gtgacggggc tctggctcac ccgctcggtg gcttgggtct tcgtgttccg cactctgcag
     5461 gggctggcgg tgacctcctg ggtctgcgcc acggtgctgt tcgcggggta tctgaggccc
     5521 gaggaggcgg tgggcgccac cgtgcagcta caggtgtgca attccggcgg cacgctggtg
     5581 gggtatcttt tgggcgggct ggtggcaaag cgattcggtt atggcagcgt ttttctggtg
     5641 gcggcgtcag gcgcggcgct ggccctggcg gtgagcgccc gcatcgccga ggcgcccccg
     5701 ggagagcggc ctttggggac aaaggcgctg ctgcgggttg gggcttcccc ttttctgctg
     5761 tttgtggcgg gcatggcagt gttcgggcaa atcattctgt cggccaccac ctggggcttc
     5821 acgccgcaga tcgccaccag tcttggggcg gacgccaccg gcatcgcggc ctgttcgatg
     5881 tgcttttcgg tggccggcct gctggcggcg ctcttggtga ctcgacgcct cagcgggtgg
     5941 ctgggggaaa aacgcactct ggtgcttgcc atggcgctgg aggcggtctt ctgcatcgcg
     6001 cagccctata acccggctct gggcacgctg tacgtcaact gttttttgaa tgggctggtg
     6061 cacaacacct cctactcgct gctgctgggc ctggcgattg agctcttccc ggagcggatg
     6121 cgggcggcgg cgatgggctg gcatcaggca atctatgcgc tggggctgct catcgggccg
     6181 gtggtggcgg gctatttcag cgagctgtac gatctcaaga ccgcatttct ggtcatcggc
     6241 ctgttgggcc tggcaggggc cgtgctggcg cagctgtttt acgcccgcgc gcgcggggcc
     6301 acgctgcggc agatggagaa agaaacgcgg taaggcttgg catgaagaca gagaaagagg
     6361 cgcctgggcg ggacgcctct ttctttctgc cagaggggag aagaaagagc atgaacaaga
     6421 ttttagagaa gcgggagctg aacgcgagca cggtgctgct gaagctggag gcgagggaga
     6481 tcgcagccaa ggcgctgccg ggg
//
LOCUS       DBMDYA010000056         6500 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3026_length_6500_cov_1.322863, whole genome shotgun sequence.
ACCESSION   DBMDYA010000056 DBMDYA010000000
VERSION     DBMDYA010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_6500_cov_1.322863"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="ACZPFK_02335"
     CDS             <1..232
                     /locus_tag="ACZPFK_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405181.1"
                     /translation="QKAFEVKLGRQSGFPFGGLVLAASWGAFHFISRGVGLELWNGVS
                     CMIFSLLAGVIYVRMKKRFFYAYALIAVGYLL"
     gene            complement(791..1276)
                     /locus_tag="ACZPFK_02340"
     CDS             complement(791..1276)
                     /locus_tag="ACZPFK_02340"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR1405182.1"
                     /translation="MKKPILGKDVFLARGAVVTGEVQLGDGVSVWHNAVLRADAAPIT
                     IGPASNIQDGCVLHVDAGHPLEVGRGVSVGHGAILHGCAVGDNTVVGMGAILLNGCRV
                     GKDCLIGAGALVTEGTVIPDGCLAYGSPARAVRPLTAEEIGHNRENALHYLALGRGCD
                     C"
     gene            1433..2482
                     /locus_tag="ACZPFK_02345"
     CDS             1433..2482
                     /locus_tag="ACZPFK_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405183.1"
                     /translation="MQELEEVLRAHAARYPAMEAADYVKLVYQTEFGGDAPAAAVPVG
                     CAGVERASDASSGPLCEPIGDGLCRVPAAALPPEAAPALDRLAAVTAGEAAASEERFL
                     EKLGLLRRLCAAGELPLGSAALEAFLTPYLAAGPRPVPHSAAYRAAYAPCYRVLRQSY
                     ADLLPVFLEAERVAARGGVLAVDGRCASGKSTLAALLGRVYGCPVFHLDDFFLTPELR
                     TPERFAQPGGNVHYERFEEEILRPFTQGKEVVFRAFDCHALAMKPPVSVPAAPFAVAE
                     GCYALHPSLRGYYAGAVFLTHSKEKQRQRLLARSGPEMLRRFEQEWIPLEERYFSACG
                     VEAQCGVTLDTTELF"
     gene            complement(2966..3712)
                     /locus_tag="ACZPFK_02350"
     CDS             complement(2966..3712)
                     /locus_tag="ACZPFK_02350"
                     /inference="COORDINATES: protein motif:HMM:NF020296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR1405184.1"
                     /translation="MMDFSAALWQGDDFAALAAHLRGELAEEGYRRFHSSLLPGVEDV
                     LGVRVPLLRALAKQIARGDFRAYLAGAYAHAHSYEETLLAGLVTGYARMGLDEALDRI
                     AAYVPHISNWALCDVAASTFKILTKDPVRGLDFAAGWMARPGEYDKRFGAVCLLDFFV
                     TEAAIDRVLALLDGTRHEGYYYKMAAAWALSVCFVKFRDKTLALFEENHLDDFTHNKA
                     LQKCRESYRVSAQDKALLLTLKRKGAQSAQ"
     gene            complement(3809..6454)
                     /locus_tag="ACZPFK_02355"
     CDS             complement(3809..6454)
                     /locus_tag="ACZPFK_02355"
                     /inference="COORDINATES: protein motif:HMM:NF027518.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR1405185.1"
                     /translation="MLALLLLACFLLLSKRRYLPAAFFYGLALLVKPQALLYGPVFAL
                     GFLLPVFFEKGRARLEAALRTLGGAMVSVGAVIVLSAPFTGSQTPVLWLLEKYFTTAS
                     SYPYASINAFNFIAALGGNWKPMTDRFLFLSWQGWGMLGILLVTAALVLLAVRSYKNG
                     RFNLPLLAAFYGCGIFTFAHQMHERYIFPAVVFLLAAWALCGDRRLWRAAALFSATLL
                     VNMAAVYYNVGGDQFLESGFSTFLVRATGLVSTAGFCWLAWLCWRLLVRGEAARPAAT
                     AAGGEKKSVYRVLSPGARRPGPARHLSAPSLDPVPRWARRDTMAILLLTLFTACLSLP
                     YLGTTNVPQNAYTPSGSGAVQVQVQLQGSGEVTSVWAYPGILLPGAASGLVTVAGEDG
                     TVLCEFALSGGSCFSWQVQDVSFPAGRLTVTVAGAPVNEIVFADAAGSALPLGSVSAG
                     GEALFDEQTTLPDRPSQLNGMYFDEIYHGRTGYETLHGLPIYETTHPPLGKDFIALGI
                     ALLGMNPLGWRIMGVLFGITMVPVFYLLARRLLKKFWPVLCVTGLFALDFMRYTQSRI
                     ATIDVFVVLFVLLGAYFMLWFCQAYVAGGRKGALLPAALGGVAFGLGCASKWTGVYAG
                     LGLAAVYFLALYGRWRHIMSAPGSEADKEITFQRDFRFAIRTGLLFYVAVPLAIYTAS
                     YIPLLFEQSGGFTFAGILRSQQVMFSYHSQLTSTHPFESRWYSWPLMLRPVWYYMGSR
                     LPAGTVASIAAFGNPVVWWGGAAALLWLVWRLVSGRCKKKPAAVFLLILFAAQYLPWV
                     FISRATFIYHYFAAMPFALLALGLLLCRLLEALPRAGRAACVGALALAAGLFVFFFPV
                     LSGLPVSTSYAALLKWLPGWGFYIL"
BASE COUNT         1138 a         2117 c         2241 g         1004 t
ORIGIN      
        1 gcaaaaggcc tttgaggtga agctggggcg acaaagcggt ttcccttttg gcgggctggt
       61 gctggcggcc agctgggggg cgttccactt tatttcccgg ggcgtgggtc tggagctctg
      121 gaacggcgtc tcctgcatga tcttttccct cctcgccggc gttatctatg tgaggatgaa
      181 aaaacgattc ttttatgctt acgccctgat cgccgtgggg tacctgctgt aaaggggtcc
      241 gcctttgggc gcgccggttt ttcgcccgtg cggccagggg aagagcggcc aaaccggggc
      301 tttccggcgg gccgttcatc gggcgctgtt ttctatctcc cccgccgcgc gcggggcggc
      361 ggcaaccgca ctttttggga cgcgccgatg cacagaagca cggggtccca aagagaaatc
      421 ccgcacaaac caaagcgccg tcaaaaaaat tgacggcgct ttgcgctttg ttttctgcgg
      481 tcccacaggg catggcggcc cctgccggcc ctgccgcgcg ccgatttttg cctgtggggc
      541 agaggggcgg caagaaaatc tgttctgccg cgtgggaatt aaacccggct ttttgcctgc
      601 gcggccaggg gtaggcgacc aaaccgaggc tttccggcgg gccgctcatc aggcgccgtt
      661 ttctgctccc gccgcgcgcg gagacggcag tcgccgccct ttttttgagc gtcgaagcgc
      721 ggtctacagg gcgcatctgc ggtttccgcg cccacaggtt ttcccgcttc ctgcggggaa
      781 agccgccgcc tcagcagtcg cacccgcgcc ccagggccaa gtagtgcagc gcgttctccc
      841 ggttgtgccc gatctcctcc gccgtcaggg ggcgcacggc cctggctggg ctgccgtagg
      901 cgagacagcc gtcggggatg accgtgccct cggtcaccag cgcgcccgcg ccgatcaggc
      961 aatctttgcc cacccggcag ccgttgagca ggatggcccc catccccacc accgtattgt
     1021 cgcccaccgc acagccgtgc aggatggccc catgccccac cgagacgccg cgcccaacct
     1081 ccagcgggtg cccggcgtcc acgtgcagca cgcagccgtc ctggatattg ctggcggggc
     1141 caatggtgat gggcgcggcg tcggcgcgca gcacggcgtt gtgccagacg ctgaccccgt
     1201 cccccagttg cacctcgccc gtcaccacag cgccccgggc gagaaaaacg tcctttccca
     1261 aaatcggttt tttcaccggg atccctctct ttcctctcgc gcagtttcct ttattgtacc
     1321 cgcgccgcgc ccgttttgca aaggcaggcc gccgccgcgg ttgaaaagac aggcgtcctt
     1381 tggtaaaata aagaccaccg cgaagctgcg cggcggaaag gagagggtat tcttgcagga
     1441 gctggaagag gtcctcaggg cccatgcggc ccgctatccg gccatggagg ccgcggacta
     1501 tgtgaagctc gtctatcaaa ctgaattcgg cggcgatgcc cccgccgcgg ctgtgccggt
     1561 ggggtgcgcc ggggtggagc gggcgtccga cgcctcaagc ggtcccctct gtgagcccat
     1621 cggggacggc ctgtgccgtg taccggcggc ggcgctgccg ccggaggcgg ccccggcgtt
     1681 ggaccggctg gctgcggtca cggccgggga ggccgcggcc agtgaggagc gctttttgga
     1741 aaaactgggg ctgctgcgcc gcctttgcgc ggcgggggag ctgccgctgg gcagtgccgc
     1801 gctggaggct tttctcaccc cctacctcgc cgccgggccg cggccggtgc ctcacagcgc
     1861 ggcctaccgg gccgcctatg ccccctgtta ccgggtgctg cggcagagct atgcggattt
     1921 gttgcccgtg tttctggagg cggagcgggt ggcggcccgc ggcggcgtgc tggccgtcga
     1981 cggccgctgc gccagcggca agagcacgct ggccgctttg ctcggccggg tgtacggctg
     2041 cccggtgttc cacctggacg acttcttttt gacgccggag ctgcgcaccc ccgagcggtt
     2101 tgcccagccg ggcggcaatg ttcattacga gcggtttgaa gaggagatcc tgcgcccttt
     2161 cacccagggc aaagaggtgg tttttcgcgc ctttgactgc cacgcgctgg ccatgaagcc
     2221 gccggtctcg gtgcccgccg cgccctttgc ggtggcggag ggctgctacg ccctgcaccc
     2281 gtccctgcgc gggtactacg cgggggcggt tttcctcacc cacagcaagg aaaagcagcg
     2341 ccagcggctg ctggcccgca gcggccccga aatgctgagg cgctttgagc aggagtggat
     2401 cccgctggaa gagcggtatt tttccgcctg cggcgttgag gcccagtgcg gggtgacgct
     2461 ggacaccaca gagctgttct gacctcccgc cccgcccgtt tcgtcaaaaa agagggcggg
     2521 tgcgtcccat ggagaggccg ccgcgtccgg ctgttctttc agttcgatgt ggccggtgcg
     2581 aaacgccgct gtaaagttga gttttctgaa aaggcatcac cccggcgggg ccttttgcaa
     2641 aagcactgcc ggatccgcgg ggcggtcaga aagagggggc ggctcggctt gaaaaatgcc
     2701 gccggggcag acggccgccg cccgggggag gcgcgggctt ttgcagcgcc gcagggcggc
     2761 ttccccgttc ccggatgcgc ggcgggccga agccgtcggc tgtgaagaaa gcttcacttg
     2821 aaaaaaggtt ggcgacccgc ggggatcgcc aacctttttg attttattgg ggcgccgggg
     2881 ccgccgggcg ggtcggtctt tccccgcatc cgtccgcctt ggggcgctgc gttttttgcc
     2941 tgctttgggg tgtccgcacg gctttttact gcgcgctttg ggcgcccttg cgtttcaggg
     3001 tcagcagcag cgccttgtcc tgcgcgctga cccggtagct ctcacggcat ttctgcagcg
     3061 ccttgttgtg ggtgaaatcg tccagatggt tctcttcaaa aagggccagc gtcttgtcgc
     3121 ggaacttgac gaaacagacc gacagcgccc aagccgcagc catcttgtag taatagccct
     3181 cgtgccgggt gccgtccagc agggccagca cccggtcgat ggcggcctcg gtcacgaaaa
     3241 aatccagcag gcagaccgcg ccgaaccgct tgtcgtattc ccccgggcgg gccatccagc
     3301 ccgcggcgaa gtcaaggccc cggacggggt ctttcgtcag gattttaaag gtactggcgg
     3361 ccacgtcgca gagcgcccag ttgctgatgt ggggtacata ggcggcgatg cgatccagcg
     3421 cctcgtcgag ccccatgcgg gcgtagccgg tgacaagccc ggccagcagc gtctcctcat
     3481 agctgtgcgc gtgggcgtag gccccggcca gataagcgcg gaaatccccg cgcgcgatct
     3541 gctttgccag ggcgcgcagc agcggcaccc gcacccccag cacgtcctcc acccccggca
     3601 gcagcgagga gtgaaaacgg cggtagccct cctccgccag ctcgccgcgc aggtgcgcgg
     3661 cgagggcggc aaagtcatcg ccctgccaga gggcggcgga aaaatccatc atcaaaagct
     3721 cctttggctg cgggcgcgcg gaggaggagt ccccccctcc cggcgcgccc ggttgttcag
     3781 agcggcccgg cgcaccggat ccgccgcgtc acaggatgta aaagccccag ccgggcagcc
     3841 atttcagcag cgcggcgtag gaggtggaca ccggcaggcc ggagagcacc gggaaaaaga
     3901 agacgaacag cccggcggcc agcgcgagcg cgccgacgca ggccgcgcgc ccggccctgg
     3961 gcagggcctc cagcaggcgg cagagcaaaa ggcccagcgc cagcagcgca aagggcatgg
     4021 ccgcaaagta gtggtagatg aaagtggcgc gggagatgaa gacccagggc agatactggg
     4081 cggcgaagag gatgagcaga aagacggcgg ccggcttttt tttgcagcgg ccggagacaa
     4141 gacgccagac cagccagagc agggccgccg ccccgcccca ccagaccacc gggttgccga
     4201 aagcggcgat gctggccacc gtgcccgcgg gcaggcggga gcccatatag taccacaccg
     4261 ggcgcagcat cagcggccag ctgtaccagc gggactcaaa ggggtgggtg gaggtgagct
     4321 ggctgtgata gctgaacatc acctgctggg agcgcaggat gccggcaaag gtaaagccgc
     4381 cgctctgctc gaacagaagc gggatatagg atgccgtgta gatcgccagg ggcacggcca
     4441 cataaaagag aagaccggtg cggatggcaa agcggaagtc ccgctgaaag gtgatctcct
     4501 tgtcggcctc gctgccgggc gcgctcatga tgtgacgcca gcggccgtac agggccagaa
     4561 aatagaccgc ggccagcccc aggccggcgt agacgccggt ccacttgctg gcgcagccga
     4621 gcccaaaggc caccccgccc agcgcggcgg gcagcagcgc ccccttgcgg cccccggcca
     4681 catacgcctg gcagaaccag agcatgaaat aggcgcccag cagcacgaac agcaccacaa
     4741 agacgtcgat ggtggcgatg cgggattggg tgtagcgcat gaaatcgagg gcgaacaggc
     4801 cggtgacgca gaggacgggc cagaattttt tgagcagccg ccgggccagc agatagaaga
     4861 cgggcaccat ggtgatgccg aacagcaccc ccatgatgcg ccagcccagc gggttcatgc
     4921 ccagcagcgc gatgcccagg gcgatgaaat ccttgcccag gggcgggtgg gtggtctcat
     4981 aaatgggcag cccgtgcagc gtctcatagc cggtgcggcc gtggtagatc tcgtcaaaat
     5041 acatgccgtt gagctggctc ggccggtcgg gcagggtggt ttgctcgtcg aagagggcct
     5101 cccccccggc ggagacggac cccagcggca gggcgctgcc cgccgcgtcg gcaaagacga
     5161 tctcgttcac cggcgcgccg gccaccgtga ccgtgagccg cccggcgggg aacgagacgt
     5221 cctgcacctg ccaggaaaag caggagcccc ccgagagcgc gaattcacag agcaccgtgc
     5281 cgtcctcccc ggcgacggtg accagcccgg aggccgcgcc cggcagcagg atgcccgggt
     5341 aggcccagac cgaggtcacc tcgccggagc cctgcagctg cacctgcacc tgcaccgcgc
     5401 cgctgcccga gggggtgtag gcgttttggg gcacgttggt ggtgccgaga tagggcagtg
     5461 agagacaggc ggtgaagagg gtgagcagca ggatggccat cgtgtcccgc cgcgcccagc
     5521 gggggacggg gtcgaggctg ggggcggaga gatgccgcgc cgggcccggg cgccgcgcgc
     5581 ccgggctcag cacccggtag acggatttct tttctccccc cgccgcggtc gcggcgggcc
     5641 gggccgcctc ccctcgcacc agcagccgcc agcagagcca ggccagccag cagaacccgg
     5701 cggtggagac gagcccggtg gcccgcacca gaaaggtgga aaagccgctc tcgagaaatt
     5761 ggtcgccccc cacgttatag tagacggccg ccatgttgac cagcagcgtg gcggaaaaaa
     5821 gcgccgccgc ccgccacagc cggcggtcgc cgcagagcgc ccaggccgcc agcagaaaga
     5881 ccacggcggg gaagatatac cgctcgtgca tctgatgggc gaaagtgaag atgccgcagc
     5941 cgtagaacgc cgccagcagc ggcaggttga agcggccgtt tttgtaagag cgcacggcga
     6001 gcagcaccag cgccgcggtc accagcagaa tgcccagcat cccccagccc tgccagctca
     6061 aaaacagaaa acggtcggtc atcggcttcc agttgccgcc cagggcggcg atgaaattga
     6121 acgcgttgat cgaggcgtag gggtaggaag aggcggtggt gaaatatttt tccagcagcc
     6181 acagcaccgg cgtctggctg ccggtaaagg gggcggagag gacgatcacc gcgccgacgc
     6241 tgaccatcgc cccgcccagg gtgcgcaggg cggcctccag gcgggcgcgg cccttttcaa
     6301 aaaagacggg cagcagaaag cccagcgcaa agaccggccc gtacagcagc gcctgcggtt
     6361 tgaccagcag agcaaggccg taaaaaaagg cggcgggcag atagcggcgc tttgacagca
     6421 gcagaaagca ggccagcagc aagagggcca gcaccgcgtc cacctgcttt caaccccgcc
     6481 tttatctttg actgtgcggt
//
LOCUS       DBMDYA010000057         6486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3037_length_6486_cov_1.758340, whole genome shotgun sequence.
ACCESSION   DBMDYA010000057 DBMDYA010000000
VERSION     DBMDYA010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_6486_cov_1.758340"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="ACZPFK_02360"
     CDS             complement(<1..1179)
                     /locus_tag="ACZPFK_02360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1405186.1"
                     /translation="MRAKKRFTAGLLALLLLLLCATAALAAGGVPGAVLRARDGVVRI
                     FSYQGKIGFSGTGFALSNDSAGAVILTNYHVVDGCSAYELYYDGNGPVELEIVAVSET
                     QDLCVLRAGRKLKGLKPLPLANGVSSGEAVYALGYPGAADELSLQMMTTKEEMTVSNG
                     IVSAIQDAGGVGNGSRRVKLVQTNTDISHGNSGGPMLNEKGQVVGVSTLSMNDFSVSG
                     INGAVHVDEVRSFLDVQGIRYQKGGADLLLLILVMVLALIAATLVTLFVVLRRRESRA
                     AKAQEAARGQAFPAPQAAVPVGAPPVWSGTEPQPPQDGTARRETAAMPGPQPPMETAP
                     APVQNRWAAAPSGNVPPAPAQGTPAAQPPPSPAAAIPAASFTAARAPAGGYGESSMPQ
                     N"
     gene            complement(1299..3485)
                     /locus_tag="ACZPFK_02365"
     CDS             complement(1299..3485)
                     /locus_tag="ACZPFK_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR1405187.1"
                     /translation="MCFSENPGDRPEENSDKSRTAVKKGLENGGIRSCWKTAALLAVF
                     VVIGLCSFGLIRTGFRLAEGYPPVYWPHPWAVGVVLTFFASLVGGFIAMGRLFWLASK
                     SHIPAPCAAGPLPGPLTLCTTGPEAGFSTRADGGPQPNAPLPPRADAVGAGDATERRA
                     PAGAPPSSPAPSAAVSAAAQGSVSSASSASAAATPTPDTAPPAAKAASASAAIPTSTS
                     AGGPPKRQPRQYDRADTVFALCAFLLGYLFLRLVTIFYAGAGVTLFAALYAALVLGYA
                     HCAGVKPSRASWGWLAFFLAMALLFFVGIATPLRLPALFFLAGVAVYWTLAVFGARLS
                     GRVDGCLGGDLRNAFWWAPFGNFSALPGALGHSLKRRGGGQKRREDTAPAVLLGAVLT
                     LFLLPLVLLLLASADASFERALSAMFGWIDFDFPAVFLRLVLAVPVSFYLFGLFWGAR
                     RRTGLRGSAEKLRQAAEKRRSLGFAAAGIPIAALLAVYLIFFIAQLPYFISAFAGLLP
                     GGFTYAEYARRGFFELLWVAAINLLLLFFTKRRVRPGGRARRVLAALLCGATLLLLGT
                     AARKLGLYIGIFGLTRKRVWAAWAMVLVAVVVVLALVEEFRRLPLARALALVCCGWFF
                     LLCAVNVDGLVVRYNYAAWQAGRIEEMDAAAPEPVFAAGALYEVGCSTADEDLRRRCE
                     EALRDADYGEQWRAESGHPVTGFTLAGLRAARLAREAGVSASVTNW"
     gene            complement(3672..3911)
                     /locus_tag="ACZPFK_02370"
     CDS             complement(3672..3911)
                     /locus_tag="ACZPFK_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405188.1"
                     /translation="MPIIVNLDVMMARRKISAGELAERVGITPANLSILKNNKARAVR
                     FSTLEALCRELHCQPADILEYREEPQPPGPPQEAL"
     gene            complement(3925..4389)
                     /locus_tag="ACZPFK_02375"
     CDS             complement(3925..4389)
                     /locus_tag="ACZPFK_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIR1405189.1"
                     /translation="MWNKNRSLLLSRIAVGIFLGLLILAVLAAPTLTKLFLDFSRAPL
                     EGLSRQQALLCFLVSEYASVFPAGALLWNLHRLLQRIGRAQVFVPANESALRVISWCC
                     LAEAAICLASAVYYIPFLLVSVAAGFMALIVRVIKNVFAEAIALKAENDYTI"
     gene            complement(4467..4700)
                     /locus_tag="ACZPFK_02380"
     CDS             complement(4467..4700)
                     /locus_tag="ACZPFK_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405190.1"
                     /translation="MRRWIVTAGLCLAAPLLGCLPPGRADPSPPQRVEWSHSACGAVG
                     GTDACGPTDTDRVLVSALVVDGRPVVIRCSPAL"
     gene            4750..5031
                     /locus_tag="ACZPFK_02385"
     CDS             4750..5031
                     /locus_tag="ACZPFK_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405191.1"
                     /translation="MKSKYFLLFFDIFLLLFSDKAAKIRFVGAYFYGRGTLFILRAAR
                     GERGWNTAGVLPRAGTNGKGLARENAAETQKKQRANAVPVGIPSLSFLL"
     gene            5450..5629
                     /locus_tag="ACZPFK_02390"
     CDS             5450..5629
                     /locus_tag="ACZPFK_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1405192.1"
                     /translation="MDKNHANHCIRCTINECANHCDCEPCCALESIEVCCCNTDRATD
                     KQCTECASFRAKNEY"
     gene            complement(6103..>6486)
                     /locus_tag="ACZPFK_02395"
     CDS             complement(6103..>6486)
                     /locus_tag="ACZPFK_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405193.1"
                     /translation="VHWTMFFYGDNGYDADFLQLDGQPQEELIESLMEEMPWRFAVLR
                     CRGEGMPEREVYAGTLPGHYYGPEPPEPDDQEGLRRLTEELVREEQLEFTDPAAFVQA
                     RLRRGRANYEAAKRRREEQEKKEQG"
BASE COUNT         1240 a         2178 c         2031 g         1037 t
ORIGIN      
        1 gttttgcggc atactggact ccccatagcc gccggccggg gcgcgggcgg ctgtaaaaga
       61 agcggcgggg atcgcggctg caggggaggg aggcggctgc gccgcaggcg tcccctgcgc
      121 cggggcaggg gggacattcc cggacggcgc cgccgcccac cggttttgca cgggggccgg
      181 ggccgtctcc atcggcggct ggggcccggg catggccgcg gtctctcggc gggccgtccc
      241 gtcctggggc ggctgcggct ctgtcccgct ccacacgggc ggcgcgccca ccggcacggc
      301 ggcctgcggc gcggggaacg cctgccctcg ggcggcctcc tgtgcctttg ccgcgcggct
      361 ctccctgcgg cgcagcacca caaacagggt caccagcgtg gccgcaatca gggccagcac
      421 catcaccagg atcagcagca gcaggtcggc gccgcccttt tgatagcgga tgccctgcac
      481 gtcaagaaag ctgcgcacct cgtctacatg caccgcgccg ttgatgccgc tgaccgaaaa
      541 atcattcatg gaaagcgtac tgacccccac gacctggccc ttttcattga gcatcgggcc
      601 gccggagttg ccgtgactga tatcggtatt ggtctgcacc agcttgaccc ggcggctccc
      661 gttgcccacc ccgccggcat cctgaatggc gctgacgatg ccgttgctca ccgtcatctc
      721 ctctttggtc gtcatcatct gcagcgagag ctcgtccgcc gcgccgggat agcccagcgc
      781 atagaccgct tcgccgctgg aaacgccgtt tgccaaaggc agcggcttga ggcccttgag
      841 cttgcgcccg gcccgcagca cgcacaggtc ctgcgtctcg gacacggcca cgatctccag
      901 ctccaccggc ccgttgccat cgtagtagag ctcatacgcc gagcagccgt ccaccacgtg
      961 gtaattggtg aggatcaccg ccccggcgct gtcgttggag agggcaaacc cggtgcctga
     1021 aaagccgatc tttccctgat aagaaaatat ccgcaccacg ccgtcccggg cgcgcagcac
     1081 agccccgggc accccgccgg cggccagcgc cgccgtggcg cacagcagca aaagcagcaa
     1141 agccagcagc ccggccgtga atctcttttt tgcacgcatg tccctcacac ctctcggtct
     1201 cttactccaa gtatatcagt tcaggcccct caaaaacagc ggcaaagctg cttttccgtc
     1261 acaaaaaaac agcggtttcc ttgtggaaac cgctgttttt accaatttgt gacagaagca
     1321 gaaacccccg cttctcttgc cagccgggcg gcgcgcaggc ccgccagcgt gaagccggtc
     1381 accggatgtc cgctctcggc gcgccactgc tcgccgtagt cggcgtcccg cagcgcctcc
     1441 tcacagcgcc gccgcaggtc ctcgtccgcc gtggaacagc ccacctcgta cagcgcgcct
     1501 gcggcgaaga ccggctccgg ggcggcggcg tccatctcct cgatgcggcc cgcctgccag
     1561 gcggcgtagt tgtaccgcac caccaaaccg tccacattca cggcgcagag caaaaagaac
     1621 cagccgcagc acaccagcgc cagcgcgcgg gccagcggca gccgccggaa ttcctccacc
     1681 agcgccagca ccaccaccac tgccaccaga accatggccc aggcggccca aacccgcttg
     1741 cgggtgagcc cgaaaatgcc gatatacagc cccagcttgc gcgccgccgt gcccaacagc
     1801 agcagtgtgg ccccgcagag cagggcggcc agcacccgcc gggcgcgccc gcccgggcgc
     1861 acgcggcgct tggtgaaaaa cagcagcagc agattgatgg ccgccaccca gagcagctca
     1921 aaaaagccgc gccgcgcata ctcggcatag gtgaagccgc cgggcagcag gccggcaaag
     1981 gcggagatga aatagggcag ctgtgcgatg aaaaagatca gatagaccgc cagcagggcg
     2041 gcgatgggga tgcccgccgc ggcgaacccc aaagagcggc gcttttccgc cgcctgccgc
     2101 agcttttcag cgctgccccg caggccggtg cgccgccgcg ccccccaaaa gaggccgaac
     2161 aggtagaacg agaccggcac cgccagcacc aggcgcagaa acacagcggg aaagtcaaag
     2221 tcgatccagc cgaacatggc gctgagcgcc cgctcaaacg aggcgtcggc gcttgccagc
     2281 aacaaaagca ccagcggcag caaaaacagt gtcagcaccg cgcccagcag cacggcgggg
     2341 gcggtgtcct cccgccgctt ttgcccgccg ccccgccgct ttaggctgtg acccagcgcg
     2401 ccgggcaggg cggaaaaatt gccgaaaggg gcccaccaga acgcattgcg caggtcgccg
     2461 cccagacagc cgtccacgcg gccggagagg cgcgcgccga agaccgccag cgtccagtag
     2521 acggccaccc cggccagaaa gaaaagcgcg ggcaggcgca ggggagtggc gatccccaca
     2581 aaaaagagca gggccatggc gagaaaaaac gccagccagc cccagctggc ccgggacggt
     2641 tttacgcccg cgcagtgggc ataccccagc accaaggcgg cgtaaagcgc cgcaaaaagg
     2701 gtcacccccg cacccgcgta aaaaatagtg accagccgca gaaacagata acccagcaga
     2761 aaagcgcaga gggcaaagac cgtgtcggcc cggtcgtact gccgcggctg acgcttgggc
     2821 gggccgcccg cgcttgtgga cgtgggtatg gccgccgatg cagatgcggc tttggctgca
     2881 ggtggggctg tatcaggtgt gggggttgcg gctgcggacg cggaggacgc ggaggacacg
     2941 gagccctgcg cggcggctga aaccgcggca gatggcgcgg gggaggaggg cggcgcgccc
     3001 gctggggcac ggcgttccgt ggcatccccc gcccccacgg catccgcccg cggcggcagc
     3061 ggcgcgttcg gctgcggccc accgtccgcc cgggtagaaa aacccgcctc cggccccgtc
     3121 gtgcacaggg tgagaggccc gggcagggga cccgcggcgc agggcgcggg tatgtggctt
     3181 ttcgacgcca gccagaaaag acggcccatc gcgatgaagc cccccaccag gctggcaaaa
     3241 aacgtcagca cgaccccgac cgcccaggga tggggccaat agacgggcgg atagccctcc
     3301 gccagccgga agccggtccg gatcaaccca aacgaacaca ggccgatcac cacaaaaacc
     3361 gccagaagag cggcagtctt ccaacaggag cggatcccgc cgttttccaa tccttttttg
     3421 acggctgtcc ggctcttgtc gctattttcc tccggcctgt caccgggatt ctctgaaaag
     3481 cacatacggc tctcctcctc tcatggagct ttttgatttt gttgaaaggg cggtgcggaa
     3541 aaatttgcgg cgtccgggcc gacgtctttc ccactcgcgc cggaattttt caactccccc
     3601 cccgcagggc ggggcaagcc gcgttttaca gcggacgacc cgcggcgccg gggaaggggg
     3661 gcggccgccc gtcacagcgc ctcttgaggc ggcccgggcg gctgcggctc ctcccggtac
     3721 tctaaaatat cggcgggctg gcagtgcagc tcgcggcaga gggcctccag agtggaaaat
     3781 cgaaccgccc gcgccttgtt gtttttgagg atggacaggt tggccggggt gatgcccacc
     3841 cgctcggcca gctcgccggc cgatatcttg cggcgggcca tcatgacgtc cagattgaca
     3901 atgatcggca tagctcccgc ccccttaaat ggtgtagtcg ttttcggcct tgagggcaat
     3961 ggcctcggcg aaaacgtttt tgatcacccg cacgatcagc gccataaatc ccgccgccac
     4021 cgacacgaga agaaaaggga tatagtagac ggcgctcgcc aggcagatgg ccgcctcggc
     4081 gaggcagcac cagctgatga cgcgcagcgc cgactcgttg gccggcacaa agacctgcgc
     4141 ccggccgatg cgctgcagca ggcggtgtag gttccagagc agcgccccgg cggggaagac
     4201 ggaggcatac tcgctgacga gaaagcagag cagcgcctgt tggcggctga gaccttccag
     4261 cggcgcgcga ctgaagtcga gaaacagctt ggtgagggtg ggcgcggcca gcaccgccag
     4321 gatcagcagc ccgagaaaga tccccaccgc gatgcgggag agcagcaggg agcggttttt
     4381 gttccacata atatcaaatt cctccttgaa tgagatcgcc gggcccgtcc ggcaggggtg
     4441 ggggacagcc gcattttaaa acggcatcac agggcgggcg aacagcggat caccacgggc
     4501 cggccgtcca ccaccagggc ggacaccagc accctgtctg tgtcggtcgg gccgcaggcg
     4561 tcggttccgc ccaccgcccc gcaagcggag tgggaccatt ccacccgctg gggcggagag
     4621 gggtccgccc ggcccggcgg caggcagccc aaaagcggag cggccaggca gaggccggct
     4681 gtaacaatcc atcgccgcat gtgggacgcc cccctttcct ttctgcggtc gagtatagca
     4741 caggatatat tgaaaagcaa gtatttttta ttgtttttcg atattttttt attattattc
     4801 agcgataaag ctgctaaaat tcgttttgtg ggcgcttatt tttatgggag ggggacgctt
     4861 tttatcctcc gcgcggcgcg cggagagcgc ggatggaaca cagcgggcgt gttaccccgc
     4921 gcggggacaa acggaaaagg cttggcccga gaaaacgcag cggagaccca aaaaaaacaa
     4981 cgggccaacg ccgtacccgt gggcattccg agcctgtctt tcctcttatg aaagcttttc
     5041 cagggaaaaa ggtatggggc ggcaagcttt tttgtatttg ctgttttgtt ctgctttcta
     5101 tcaggcaatt tttgtggacg gctccgtcgc ctttcgcttt tgtgggaacc gaagcgaaaa
     5161 gccccctaaa aatagccaca ggctattttt aggggggaat agccccgggg gtcaggggac
     5221 atactaaaac cagacgcggg gcaggggcgc gggcggcagg gttttgcccg atcggctccc
     5281 aaaaggccct tgcgcgcttt tcggttttaa ggctgcgggc cgttttcgcc cgcgcggtca
     5341 ttttttcagc cgcctgcctt gaccaccgcc cacggcggca agcggagccc ttaaaataag
     5401 tttttacaag aaccataaaa acccatctga cttttaggag gaactgaaca tggacaaaaa
     5461 tcatgccaac cactgtatcc gctgcaccat caacgagtgc gccaaccact gcgactgcga
     5521 gccctgctgc gcgctggaat ccatcgaggt ctgctgctgc aacaccgata gggccaccga
     5581 caagcagtgc accgagtgcg cttctttccg cgccaaaaac gagtactgag agaccggcaa
     5641 aaaaggccgg ccccgggcga tgctcttcgc ctgaggccgg tctttttcaa ttgatggaaa
     5701 aggtggaaaa attgcagaaa actccccggc ccccgcagcg gggagcctgt gcttttgctt
     5761 ttggcccttt tgctgcggcg ggatcgcgcc ggtagagctt tcaccgcggc gatgccggat
     5821 ttttgcgttc cccggtccgc cccccgggcg gcgttgcagg gccgtctctc gtggcaaggg
     5881 aaatcaggat gccgttttac cgggggagtc gggatgcgcg cgctttgagc cgcgcgcctc
     5941 gccgcaggcg gcgtttctct gatttcacca ttgtcagcag cggggcattt ccctatcccc
     6001 ccgatttcat ccccaccggc ggcgcatccc cagttgcccc tctccgggga gcctgcggca
     6061 agggccgtct gaaagacttc cgccggcggc cgcggtgggg cctcacccct gttctttttt
     6121 ctcctgttcc tccctccggc gctttgcagc ctcatagttg gcccggccgc gtctcaggcg
     6181 cgcctgcaca aaagccgccg ggtcggtgaa ctccagctgt tcttcccgca ccagctcctc
     6241 cgtcagacgg cgcaggcctt cctggtcgtc cggctccggc ggctccggac cgtaataatg
     6301 gcccggcagg gtgcccgcgt acacctcccg ctccggcatc ccctcgcccc ggcagcgcag
     6361 cacggcaaag cgccagggca tttcctccat cagcgactcg atcagctctt cctgcggctg
     6421 cccgtccaac tgcagaaagt ccgcgtcgta cccgttgtcc ccgtagaaga acatcgtcca
     6481 gtggac
//
LOCUS       DBMDYA010000058         6483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3041_length_6483_cov_1.536196, whole genome shotgun sequence.
ACCESSION   DBMDYA010000058 DBMDYA010000000
VERSION     DBMDYA010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_6483_cov_1.536196"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="ACZPFK_02400"
     CDS             <1..565
                     /locus_tag="ACZPFK_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1405194.1"
                     /translation="GQSPHTLDRQKNARVFDPRLGGGVLADIGVYALSFVQMLAGHKP
                     VLIRNRAALDRETGVDLRDDILLKYNSGLEAHITVSGEEELPPLVEVVGSRGSLICEE
                     GLFSGKFAVKTPEGRFDYNFAAERFRYYYAYELEHFARCLLEGRRESYIVPLRDTLHV
                     MQIMDACRRMAGCADPQETELERHSAV"
     gene            600..1631
                     /locus_tag="ACZPFK_02405"
     CDS             600..1631
                     /locus_tag="ACZPFK_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1405195.1"
                     /translation="MKKIMAIALSLALALSLAACGGDSSSASAPAAGSAGSGSAASTA
                     GGAKQYKIAYAFANMDENNMRTLTGLNKAIDEMNATGEYDIELIYTDSQSQVDKQISD
                     VESLIQQQPDLILVSAVDTVGSIPCLEAVKAAGIIAVDDRGMDSDAKDLKWTGFDEDT
                     IGQLKAQAMKDYLEANPDVHLNVGGIYGLASQSEQLKRVQCILDLAEEMPDRITILET
                     QYCDWSTDRATSTVEDWLQRYPEMNCIVTASDDMGLGACNALNAAGKTGWYVDGVDGT
                     QIGIQLAEDGTQYYNTIAANQEAITKQMVEIFIQKIDGAYTEPEYSCPAECFTIVNPD
                     TVADYNTSI"
     gene            1701..3215
                     /locus_tag="ACZPFK_02410"
     CDS             1701..3215
                     /locus_tag="ACZPFK_02410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405196.1"
                     /translation="MSDEVILKMEGITKLFPGVKALDKVDLELRKGEVLAVIGENGAG
                     KSTLMKVLLGIYQAEEGTITYKGKEVRFSSPLAALGAGIAMIHQEISLVPTMDVAENI
                     WLGRENQTMKHGMIDRQNRYRMTAEILRQMDIDLDPRAMVKDLTVAKMQMVELVRAAS
                     YNADVIIMDEPTSALANEEIATLYRIVRDLQKKGVSIIFISHKLEEIFEICDRVSVYR
                     DGHYVATRACDDITMQELVTMIVGREVTSQFPKMKADIGEVVLEVRDFNSLGVFSDVS
                     FSVRRGEILGISGLVGAGRSEIMRALFGADRRDSGKVFIEGKEVKITSPTDAIRNGIS
                     MLTEDRMRTGSIYTMSVKGNTTIAAFGRTCCNKLGIINSKKENQAFESVRGDLEVKCA
                     SERQLIKELSGGNQQKVLVGRWLLTRPKVLIVDEPTRGIDVGSKSEIHKLISKLACEG
                     MAVIMISSELPEILGMSDRILVVRHGRIVYECDREGATQETLITHAFGAAEARS"
     gene            3248..4246
                     /locus_tag="ACZPFK_02415"
     CDS             3248..4246
                     /locus_tag="ACZPFK_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405197.1"
                     /translation="MQNQKVHKKSFLDTRVGAFLMNNKALVILLLLCVVVSCISDVFL
                     TPSNLLNVIRQVAYSVTLACGFTLVLSSGNMDLSIGCEVGLVGICMGKMLVAGAPLLL
                     TLLASLVLGAIFSSVNALVINSFDLPPFIVTLATMSTFKGATYIATKMVPVSNLPQQF
                     VNIGQGYTFGIPNQVYIMFFMVILMAFILNKTKFGRYALAMGGNREATRLSGINTKLI
                     RWGVYAVMGVYVAVAAVMLTARSASAQVSAGQNTEMDAIAAVVVGGTPMSGGSANVVG
                     TLFGCLIVGVINNGLNLMGVDANWQLIAKGLMILFAIVLDVVSGKIATRMSKKRMA"
     gene            4355..5518
                     /locus_tag="ACZPFK_02420"
     CDS             4355..5518
                     /locus_tag="ACZPFK_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1405198.1"
                     /translation="MKTLRVGVIGAGMMGRQHVEAARRIPGVEVVALADANAALAART
                     CEELCIPGCFADYRQMLDEAAPDAVHNCTPNNLHFEINRELLRRGVNVYCEKPLANTS
                     AETAELCALAEAAGVKAGVNFNYRQNAMVREMHERAHRVDDDPAENWGRTYLVRGHYI
                     QDWMMYDSDYNWRCDPAVGGPSRTVADVGSHWFDTVQFITGKRIVRVNAELATVLPQR
                     KRFAAQGATFAGQTGGAYELVDIGTEDAAFVMMEFEDGVKGIVTLSQVTGGHKNDLEI
                     SVDGSNYSMTWRQEEADKLWIGARPFGTIQKHAGPEMLQGDAVRYATLPSGHPVGWHD
                     ALRNGIREFYAAVRGGAPDNYATFRDGDQVVRIVEACLKSSREKKWADVEVPR"
     gene            5515..>6483
                     /locus_tag="ACZPFK_02425"
     CDS             5515..>6483
                     /locus_tag="ACZPFK_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1405199.1"
                     /translation="MKIGVLTVPFSSQPLEEVLPYLRGLGVRAVELGTGGVTNKAHCD
                     PALLLGHPERIARLKGLLRDNDMQISALSCHGNPVHPDAARAAADHQAFLDTVALAQE
                     LEVDTIVTFSGCPGDSPDSHYPNWVTCPWPDDFQKVLDYQWNGRLLPYWEKAARIARG
                     GGVRVAIEMHPGFCVYNTETMLRLRAAVGDAVGANFDPSHLFWQGMDAVAAIRELGDA
                     IHYAHAKDCRVDEGNTGRAGVLDTKHYSDVLHRAWVFRTVGYGHGEQVWRDIFSNLRL
                     VGYDGVVSIEHEDSLMSVNEGLEYVEDHSYSDKVISACYRDKDCEDS"
BASE COUNT         1264 a         2072 c         2137 g         1010 t
ORIGIN      
        1 gggacagagc cctcatacgc tggaccggca aaagaacgcc cgcgtctttg acccgcggct
       61 gggcggcggg gtgctggcgg acatcggggt ctacgcgctc agctttgtgc agatgctggc
      121 cgggcacaag ccggtgctga tccgcaaccg ggcggcgctg gaccgagaga ccggcgtgga
      181 cctgcgcgac gacatccttt taaaatacaa cagcggcctc gaggcgcaca tcacggtgag
      241 cggcgaggag gagctgccgc ccctggtgga ggtggtgggc agccgcggct cgctgatctg
      301 cgaggaggga ctgttctccg gcaaattcgc ggtgaagacc cccgaggggc gctttgacta
      361 caacttcgcg gccgagcgct ttcgatacta ttacgcctac gagctggagc actttgcccg
      421 ctgcctgctg gagggcaggc gcgagagcta catcgtcccg ctgagggaca cgctgcacgt
      481 gatgcagatc atggacgcct gccgccgcat ggcggggtgc gccgacccgc aagagaccga
      541 acttgagcgg cacagcgccg tttgagaata aaaagacgtc attcacagga ggaaaaaaga
      601 tgaaaaagat catggcaatc gctctttccc tggcattggc cctctccctg gccgcctgcg
      661 gcggggacag ctcgtccgcc agcgccccgg cggcgggcag cgccggctcc ggctctgcgg
      721 cctcgacggc cggcggcgcc aagcagtaca agatcgccta cgcctttgcg aatatggacg
      781 agaacaacat gcgcaccctg accggcctga acaaggcgat cgacgagatg aacgccaccg
      841 gcgagtacga catcgagctg atctacaccg actcccaatc ccaggtggac aaacagatct
      901 cggacgttga gtccctgatc cagcagcagc cggacctgat tttggtctcc gccgtggaca
      961 cggtgggctc catcccctgc ctggaggcgg tcaaggccgc gggtatcatc gccgtggacg
     1021 accgcggcat ggactcggac gccaaggacc tgaagtggac cggctttgac gaggacacca
     1081 tcggccagct gaaagcgcag gccatgaaag attacctcga ggccaacccc gacgtgcacc
     1141 tgaacgtggg cggcatctac ggcctggcct cccagagcga gcagctcaag cgcgtgcagt
     1201 gcatccttga cctggcagaa gagatgcccg accgcatcac catcctggaa acccagtact
     1261 gcgactggtc caccgaccgc gccacctcca ccgtggagga ctggctgcag agataccctg
     1321 agatgaactg catcgtgacc gcctccgacg acatgggcct cggcgcctgc aacgccctga
     1381 acgccgccgg caagaccggc tggtacgtgg acggcgtcga cggcacccag atcggcatcc
     1441 agctggccga ggacggcacc cagtactaca acaccatcgc cgcgaatcag gaggccatca
     1501 ccaagcagat ggtggagatc ttcatccaga agatcgacgg cgcctacacc gagcccgagt
     1561 acagctgccc ggcggagtgc ttcaccatcg tcaacccgga cacggtcgcc gattacaaca
     1621 cgtcgatctg atgcgaaaaa gttggggcag cacctgcccc aactttttaa aatcgctgaa
     1681 caagaaggag cagtatccac atgagtgacg aagtaatttt aaaaatggag ggcatcacca
     1741 agctgttccc cggcgtcaag gcgctggaca aggtggacct ggagctgcgc aagggcgagg
     1801 tgctggccgt gatcggcgag aacggggccg gaaaatccac gctgatgaaa gtgctgctgg
     1861 gcatctatca ggccgaggag ggcaccatca cctacaaggg caaggaggtg cggttcagca
     1921 gcccgctggc ggcgctgggc gcgggcatcg ccatgatcca ccaggagatc agcctggtgc
     1981 ccaccatgga cgtggccgag aacatctggc tggggcgcga gaaccagacc atgaagcacg
     2041 gcatgatcga ccggcagaac cgctatcgca tgaccgccga gattttgcgg cagatggaca
     2101 tcgacctgga ccccagggcc atggtcaagg acctgaccgt ggccaagatg cagatggtgg
     2161 agctggtgcg ggcggcgtcc tacaatgccg acgtcatcat catggacgag cccacctccg
     2221 ccctggccaa cgaggagatc gcgaccctgt accgcatcgt gcgcgatctg cagaaaaagg
     2281 gcgtctcgat catcttcatc tcccacaagc tggaggagat cttcgagatc tgcgaccggg
     2341 tgtcggtcta ccgcgacggc cactatgtgg ccacccgcgc ctgcgacgac atcaccatgc
     2401 aggagctggt gaccatgatc gtggggcgcg aggtgaccag ccagttcccc aagatgaaag
     2461 ccgacatcgg cgaggtggtg ctggaggtgc gggacttcaa cagcctgggc gttttcagcg
     2521 acgtcagctt ttcggtgcgc cggggcgaga ttttgggcat cagcggcctg gtgggggccg
     2581 gccggtccga gatcatgcgc gcgctgttcg gcgccgaccg ccgggattcc ggcaaggtct
     2641 tcatcgaggg caaggaggtg aagatcacca gccccaccga cgccatccgc aacggcatct
     2701 cgatgctcac cgaggaccgc atgcgcaccg gctcgatcta caccatgtcg gtgaaaggga
     2761 acaccaccat cgcggccttt ggccgcacct gctgcaacaa gctgggcatc atcaactcaa
     2821 aaaaggaaaa ccaggccttt gagagcgtgc gcggcgacct ggaggtgaag tgcgcctccg
     2881 agcggcagct catcaaggag ctgtccggcg gcaaccagca aaaggtgctc gtgggccgct
     2941 ggctgctcac ccgccccaag gtcctgatcg tcgacgagcc cacccgcggc atcgacgtgg
     3001 gctcaaaatc cgagatccac aagctgatca gcaagctggc ctgcgagggc atggcggtca
     3061 tcatgatctc ctccgagctg cccgagattc tgggcatgag cgaccgcatc ctggtggtgc
     3121 gccacggcag gatcgtatat gagtgcgacc gggagggcgc gacgcaggag accctgatca
     3181 cccacgcgtt cggcgccgcc gaagcgcggt cctaaccccg aaaaagacga aaggacttga
     3241 atcagatatg caaaaccaaa aagtacataa aaaaagcttt ctggataccc gggtcggcgc
     3301 tttcctgatg aacaacaagg ctcttgtcat cctgctgctg ctctgcgtgg tggtgtcctg
     3361 catctcggac gtgtttctga ccccgtcgaa cctcttgaac gtcatccgcc aggtggccta
     3421 ctcggtcacg ctggcctgcg gcttcaccct ggtgctcagc tcgggcaaca tggacctgtc
     3481 cattggctgc gaggtgggcc tagtgggcat ctgcatgggc aaaatgctgg tggccggcgc
     3541 gccgctgctg ctgacgctgc tcgcctcgct ggtgctgggc gcgatcttca gctcggtcaa
     3601 cgccctggtc atcaactcat tcgacctgcc gcccttcatc gtcaccctgg ccaccatgtc
     3661 cacgttcaag ggcgcgacct atatcgccac caaaatggtg ccggtctcaa acctgcccca
     3721 gcagttcgtc aacatcggcc aggggtacac gttcggcatc cccaaccagg tctacatcat
     3781 gttcttcatg gtgatcctga tggcctttat cctgaacaag accaagttcg gccgctacgc
     3841 gctggccatg ggcggcaacc gcgaggcgac ccgcctgtcc ggcatcaaca ccaagctcat
     3901 ccgctggggc gtctacgccg tcatgggcgt gtacgtggcg gtggccgcgg tcatgctgac
     3961 cgcccgctcg gcctcggcgc aggtctcggc cggccagaac accgagatgg acgccatcgc
     4021 ggccgtggtc gtgggcggca ccccgatgtc cggcggctcg gccaatgtgg tgggcaccct
     4081 gttcggctgc ctgatcgtgg gcgtcatcaa caacgggctg aacctgatgg gcgtggacgc
     4141 caactggcag ctcatcgcca agggcctgat gatcctgttc gccatcgtgc tggacgtggt
     4201 ctcgggcaag atcgccaccc gcatgagcaa aaagcggatg gcctgagacc cgcgccccgg
     4261 ggcgagcggg gcgcaagaga gaaaagacag ccgtgaccgc cgcgcgggcc ctgccggcgc
     4321 gggcggcggc cgcggcccga ggaggctgaa gagcatgaag acactgagag tcggcgtgat
     4381 cggcgccggc atgatggggc gccagcacgt ggaggcggcg cggcgcatcc cgggcgtcga
     4441 ggtggtggcg ctggcggacg ccaacgcggc gctggccgcc cgcacctgcg aggagctgtg
     4501 catccccggc tgctttgccg actaccggca gatgctggac gaggcggcgc cggacgcggt
     4561 gcacaactgc acccccaaca acctgcactt tgagatcaac cgcgagcttc tgcgccgggg
     4621 cgtcaacgtc tactgtgaaa agccgctggc caacacctcg gccgagacgg ccgagctgtg
     4681 cgcgctggcc gaggcggcgg gggtgaaagc gggcgtcaac ttcaactacc gccagaacgc
     4741 catggtgcgc gagatgcacg agcgcgccca ccgggtggac gacgacccgg ctgaaaattg
     4801 gggccgcacc tatctggtcc gcggccacta catccaggat tggatgatgt acgacagcga
     4861 ctacaactgg cgctgcgacc ccgcggtggg cggcccctcc cgcaccgtcg cggacgtggg
     4921 ctcccactgg ttcgacaccg tgcagttcat caccggcaaa aggatcgtgc gggtgaacgc
     4981 cgagctggcc accgtgctgc cgcagcgcaa gcgctttgcg gcccaggggg ccacctttgc
     5041 cggccagacg gggggcgcgt acgagctggt ggacatcggc accgaggacg ccgccttcgt
     5101 catgatggag ttcgaggacg gcgtcaaggg gatcgtgacc ctctcgcagg tcaccggcgg
     5161 ccacaagaac gacctggaaa tctcggtgga cggcagcaac tattcgatga cctggaggca
     5221 ggaggaggcg gataagctgt ggatcggcgc gcgccccttc ggcaccatcc aaaagcacgc
     5281 cgggccggag atgctgcagg gggacgccgt gcgctacgcc acgctgccct cgggccaccc
     5341 ggtgggttgg cacgacgcgc tgcgcaacgg catccgtgag ttctatgcgg cggtgcgcgg
     5401 cggcgcgccg gacaactacg ccaccttccg cgacggggat caggtggtgc gcatcgtgga
     5461 ggcctgcctc aagagcagcc gcgagaaaaa atgggccgac gtggaggtgc caagatgaag
     5521 atcggcgtat tgaccgtgcc cttttcctcc cagccgctgg aggaggtgct gccctacctg
     5581 cgcggcctgg gggtgcgggc ggtggagctg ggcaccgggg gcgtgaccaa caaggcccac
     5641 tgcgaccccg cgctgctgct gggccacccg gagcggatcg cccgcctcaa ggggctgctg
     5701 cgggacaacg acatgcagat ctcggcgctg tcctgccacg gcaacccggt gcacccggac
     5761 gcggcgcggg cggcggcgga ccaccaggcc tttctggaca cggtggccct ggcgcaggag
     5821 ttggaggtgg acaccatagt caccttttcg ggctgcccgg gcgacagccc ggactcccac
     5881 taccccaact gggtcacctg cccctggccg gacgattttc aaaaggtgtt ggattatcag
     5941 tggaacggca ggctgctgcc ctattgggaa aaagccgccc gcatcgcccg cgggggcggc
     6001 gtgcgggtcg ccatcgagat gcaccccggc ttttgcgtgt acaacaccga gaccatgctg
     6061 cggctgcgcg ccgctgtggg cgacgcggtg ggggccaact tcgaccccag ccacctgttc
     6121 tggcagggca tggacgcggt ggccgccatc cgcgagctgg gcgacgccat ccactacgcc
     6181 cacgccaagg actgccgggt ggacgagggc aacaccggcc gcgccggcgt gctggacacc
     6241 aagcactaca gcgacgtgct gcaccgggca tgggtcttcc gcaccgtggg ctacggccac
     6301 ggcgagcagg tctggcggga catcttctcc aacctgcggc tggtgggcta tgacggcgtg
     6361 gtcagcatcg agcacgagga ctccctcatg tcggtcaacg aggggctgga atatgtggag
     6421 gaccacagct acagcgacaa ggtcatctcc gcctgctacc gagacaagga ctgtgaggat
     6481 tcg
//
LOCUS       DBMDYA010000059         6435 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3069_length_6435_cov_1.425008, whole genome shotgun sequence.
ACCESSION   DBMDYA010000059 DBMDYA010000000
VERSION     DBMDYA010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_6435_cov_1.425008"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="ACZPFK_02430"
     CDS             complement(<1..181)
                     /locus_tag="ACZPFK_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405200.1"
                     /translation="MNKKDNQALEVLAQAIRDGVKEELKHAPFDRTTEGTVLEALGGS
                     RYKVAAFGSVYTVSTG"
     gene            complement(275..4138)
                     /locus_tag="ACZPFK_02435"
     CDS             complement(275..4138)
                     /locus_tag="ACZPFK_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405201.1"
                     /translation="MLTLFDSKNFYEAGEGADKLRGKLTLLGKTRDTVVRDWKSTEGL
                     GGKLAGFFTSQDSKDAQAQIDSDVAALKELQKLYDNTKDEQKFDQKSAIAATLSSASE
                     AAQEYAETTGAANLNIGEFANSQAKATASSLGLQLATSALNSALTMGLSFALEAAVAG
                     IVHLVQMQEEQRRAALEAGEAFERQSGDVDAYISRVTELRTALDNGSLSQNEAYEARK
                     ELLSIQNEIIGQYGKETGAIDLLNGSLDAQTEKLKNISTATAEQNLTANASAYDKAAR
                     KMEEEKSYYLGTVYEDDAMDQLLAEFDGLVEADSNGDGTVNLRVNANARDAERIISEF
                     AARARELLAPDTGNAVTIKGYAEDTLGSVQKIIEKYGSLYDDFNLYRILTDKADADDP
                     TNVAYGDLMNGLLSAQKEYEEAVARGDQEAVERSLANLQAAKEKIAQADENGHLEEGV
                     REYLDGIADAIDDETAADRFKINFKANTDDLQDAMQTLEGLDADAILALNSGEGAQEQ
                     KKAYENLAAAAEEYGVSIDDLIGWLVDMGEVEGRVSEKMQAFTPNFDAFENSLGVIGQ
                     YQDSLQQLEAAQEQFNDIGHLTADTLKALSDNNLLQYLDFSSGSMQINTDALLDNAEA
                     AKVAAIEKLKDAAAADLQLLAEGRISEMSPIAQAALQNIGEANAIAGEKSAQAAAGYF
                     TSSAGIAAMIDTATGGNGATEKFTAQAEAVLSAYGGFAENISKIDISSASHNRAAAGV
                     DAYTESLKREKEALEAEKEALEASKSALEDKKAVYESALRAVNKALDGEIDRLQELGK
                     AEKEALEGQLDDLERAHDAAVKIIDDQIKKLEDQQQAWDDYYQPMIDAIQEQIDALEK
                     KNDEEERALKLQKAQDAYERARTQKTVRLYTHDQGFIWAADEGAVQDAQGELDQARRD
                     KEAADLAGQKQQLEDALEREKEKLEESISSYEAYKEKWEEITSEYADAQDRQILVALF
                     GKEIEQQIIDQKGVAYEEFRDRYLLVQEQLTNVEAEIAANEERIAQLEAIKQAWADAA
                     DAYESEQDRLHAAQLLGAGWEKEILDGRLETLATFKTGYYQLQQDIAKIAEQIAAVTE
                     KIAQKAAEAEAAAQRVANAAAVTGSQAGNAHISSYAVYNQIGKEVTRGLSLEKARQLI
                     ITAERTGSSGWSMQKLARGTLSAHGGLANVDEELGTKHFPELVVRPKQGRYTILDPGD
                     GVVPADISRTLFRAAVDPTGFIAAQMSALFSRGQAPALSGAQGGDIQVSSGDIHLSGV
                     QNESDLASAIVQRLPNVLLQKIRRR"
     gene            4220..4801
                     /locus_tag="ACZPFK_02440"
     CDS             4220..4801
                     /locus_tag="ACZPFK_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405202.1"
                     /translation="MTNEEKKRIWIEERMAPFKFAVCPRCGSILKTKLEKDVCDYCGC
                     TEAISIDRDERHRLLEREFDGSVSDNWEELDTYREYLAKKYAFPDERFDRKLYDERLE
                     ADAKEYEQMLKEREIATAKLEYMKAHPKCPACGSGNVREGVTSGGFDARGASIGGFFA
                     GTTGALVGGMSGGEEVFSLCLDCGHRWDVRKIW"
     gene            complement(5179..5637)
                     /locus_tag="ACZPFK_02445"
     CDS             complement(5179..5637)
                     /locus_tag="ACZPFK_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405203.1"
                     /translation="MAETYELKAKLVFSGTAKDAEAAMKPIKDYFDRHPVKLPFGVDE
                     AATAAQINAGLQRVVNGRLDLPKVKLEAALDATKLAAQAKAVSEKALAQTGGTGAASK
                     GMKAAFEQLDKLELKLKSLRSNSLAALLSGDKSAGKAIMPSYANCEAMAA"
     gene            complement(5723..6169)
                     /locus_tag="ACZPFK_02450"
     CDS             complement(5723..6169)
                     /locus_tag="ACZPFK_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405204.1"
                     /translation="MMTADQLKNAAALVGALTTLFGFCLALVKPLRTRLKTRVKSEIR
                     KITRSAESEELLQKTHDLLQRQLDADARWKADIEKQMALQNETAILQLRNTINHIYEK
                     NLPEKAFSIREKENIVDMYAQYKKLGGNHYVERIYKEMLEWETISD"
BASE COUNT          971 a         2000 c         1898 g         1566 t
ORIGIN      
        1 ggccggtgga cacggtgtac accgagccaa aggccgccac cttatagcgc gagccgccca
       61 gcgcttccag caccgtgccc tccgtggtgc ggtcaaacgg ggcgtgcttc agctcttctt
      121 tcacaccgtc ccggatcgcc tgtgccaata cctccaaggc ctggttgtct tttttgttca
      181 ttttgtcacc tcctcgatga aagaccgtcg cgcggccatc ctttgggccg ttccgccggc
      241 ggccgcgcgg cggctttgga cgttttcaac gggctcaacg ccgccggatc ttctgcagca
      301 gcacattggg cagccgctgt acaatggcgc ttgccaggtc ggactcgttc tgtacgccgc
      361 tcaggtggat atccccgctg ctcacctgga tatccccgcc ctgcgccccg ctcaaagcgg
      421 gagcctggcc gcggctgaaa agggctgaca tctgcgcggc gataaacccg gtggggtcca
      481 cggcggcgcg gaacagggtt ctgcttatat ccgcgggcac cacgccgtcg ccggggtcca
      541 aaatggtgta acggccctgc tttggccgca cgaccagctc aggaaagtgc tttgtaccca
      601 gctcctcgtc cacgttggca aggccgccat gggcggataa ggtgcctcgg gccaacttct
      661 gcatactcca accgctgcta ccggttcttt ctgcggtaat tatcagttgc cgggcttttt
      721 ctagagaaag cccgcgagta acctctttgc cgatttgatt gtacactgca tagctggaaa
      781 tatgggcatt accggcttgc gaccctgtca cggctgcggc gtttgccacc ctttgcgcgg
      841 cggcttcggc ctcagcggct ttttgagcga ttttttcggt gacggcggca atctgctcgg
      901 cgatcttcgc aatatcctgc tgcagctggt aatagccggt tttgaacgtg gccagcgtct
      961 cgagccgccc gtccagaatc tccttttccc agcccgcccc cagcagctgt gccgcgtgca
     1021 gccggtcctg ctcgctttca taggcgtcgg cggcgtccgc ccaggcctgc ttgatggcct
     1081 caagctgtgc gatgcgctcc tcgtttgcgg cgatctccgc ctccacgttg gtgagctgct
     1141 cctgcaccaa aagatagcgg tcccgaaact cctcgtaggc gacgcctttc tggtcgatga
     1201 tctgctgttc gatctccttg ccaaagaggg cgaccaaaat ctgcctgtcc tgggcgtccg
     1261 cgtactcgga ggtgatctct tcccattttt ccttgtacgc ctcgtaagag ctgatcgact
     1321 cctccagctt ttccttttcg cgctccaggg cgtcctccag ctgctgcttt tgcccggcca
     1381 ggtcggcggc ctctttgtcg cggcgcgcct ggtccagctc gccctgtgcg tcctgcacgg
     1441 cgccctcgtc cgcggcccag ataaagccct ggtcgtgggt gtacagccgc acggtctttt
     1501 gggtgcgggc gcgctcgtag gcgtcctggg ccttttgcag cttcagcgcc cgttcttcct
     1561 cgtcgttctt cttttccagc gcgtcgatct gctcttgaat ggcgtcgatc atcggctggt
     1621 aatagtcgtc ccaggcctgt tgctgatctt ccagcttttt gatctggtcg tcgatgatct
     1681 tgacggcggc gtcgtgggcc cgctccaggt cgtccagctg gccctccagc gcctcctttt
     1741 ccgctttgcc tagctcttgc agccggtcga tctccccgtc aagggccttg ttgacggccc
     1801 gcagagcgga ttcatagacc gcctttttgt cctccagcgc ggattttgag gcctccagcg
     1861 cttccttttc ggcttccagg gcctcttttt cccgcttgag ggattccgtg taggcgtcca
     1921 ccccggcggc ggcgcggttg tggctcgcgg agctgatgtc aatttttgag atgttttcgg
     1981 cgaagccgcc ataggccgag agaacggcct ccgcctgcgc cgtaaatttt tccgtggcgc
     2041 cgttgccccc ggtggcggtg tcgatcatgg cggcgatacc ggcgctggag gtaaagtacc
     2101 cggcggcggc ctgcgccgat ttttcgcccg ctatggcgtt ggcctcgcct atgttttgca
     2161 gcgcggcctg cgcgatgggg gacatttcag agatccggcc ctccgccaaa agctgcaggt
     2221 cggcggcggc cgcgtctttc agcttctcga tcgccgccac cttggcggcc tcggcgttgt
     2281 cgagcagcgc gtccgtgttg atctgcatcg agccggagct gaaatcgaga tattgcagca
     2341 ggttgttgtc gctcagggct tttagggtgt cggcggtcag atggccgatg tcgttgaact
     2401 gctcctgggc ggcctcgagc tgctgcaggc tgtcctgata ctggccgatg acccccaagc
     2461 tgttttcaaa ggcgtcaaag ttgggggtga aagcctgcat cttttccgat acgcgcccct
     2521 ccacctcgcc catgtcgacc agccagccga tcaggtcgtc gatggaaacg ccgtattctt
     2581 ccgcggctgc cgccaggttc tcataggcct ttttctgttc ctgggcgccc tcgccgctgt
     2641 tcagcgccag gatggcgtcg gcgtccaacc cctccagcgt ctgcatggcg tcttgcaaat
     2701 cgtcggtgtt tgctttaaaa ttgatcttga aacgatccgc ggcggtctcg tcgtcgatgg
     2761 cgtcggcaat gccgtccaga tattcgcgca ccccctcctc caaatggccg ttttcgtcgg
     2821 cctgcgcgat cttctccttg gcggcctgga ggttggccag cgagcgctcg acggcctcct
     2881 ggtcgccccg ggcgacggcc tcttcatact ccttttgcgc gctcaaaagg ccgttcatca
     2941 ggtcgccgta tgccacgttc gtcgggtcgt ccgcgtccgc cttgtcggtg aggatgcggt
     3001 agaggttgaa atcgtcgtac agagagccgt atttctcgat gattttctgc accgagccca
     3061 gggtgtcctc ggcataaccc ttgatggtga ccgcgttgcc ggtgtccggc gcgagcagct
     3121 cccgtgcgcg ggcggcaaac tcgctgatga tccgctccgc gtccctggcg ttggcgttga
     3181 cgcgaaggtt cacggtgccg tcgccgttgg agtccgcctc taccaggccg tcgaactcgg
     3241 ccagcagctg gtccatggcg tcgtcttcat agacggtgcc gagataatag gacttttcct
     3301 cctccatttt gcgcgcggcc ttgtcgtagg cggacgcgtt cgccgtcagg ttttgttccg
     3361 cggtggcggt ggagatgttt ttcagctttt ccgtctgcgc gtccagcgag ccgttcagca
     3421 gatcgatggc cccggtctct ttgccgtact ggccgatgat ctcgttctgg atggacagca
     3481 gctctttgcg cgcctcgtag gcctcgttct ggctcaggct gccgttgtcc agggcggtgc
     3541 gcagctcggt gacccggctg atataggcgt ccacatcgcc gctctgccgc tcaaacgcct
     3601 cgcccgcctc cagggcggcg cggcgctgct cctcctgcat ctgcaccagg tggacgatgc
     3661 ccgccacggc ggcctccaag gcgaaagaca gccccattgt cagcgctgag ttcagcgctg
     3721 aggtggcgag ctgcagcccc agcgagctgg cggtcgcctt tgcctgcgag ttggcgaact
     3781 cgccgatgtt gagatttgcc gccccggtgg tctctgcata ctcctgtgcc gcctcggagg
     3841 cggaggaaag ggtggcagcg atggcgcttt tttgatcaaa cttttgttcg tcttttgtat
     3901 tatcatacag cttttgcagc tctttcagtg ccgccacgtc gctgtcgatc tgggcctgcg
     3961 cgtccttgct gtcctgcgag gtgaaaaagc ccgccagctt gccgccgagc ccctcggtgc
     4021 tcttccagtc ccgcaccacc gtatctcggg tcttgcccag cagcgtgagc ttgccccgca
     4081 gtttgtccgc gccctcgccg gcctcataaa aatttttgct gtcgaataaa gtcaacatcg
     4141 gttgttccgc cggctgcacc gtaggggatg cagtcggcgc ggttatatga ttcaaaaatt
     4201 ttcagggggg gaagagaaaa tgacaaacga agaaaaaaag cggatttgga tcgaagagcg
     4261 gatggcgccg ttcaaatttg cggtgtgccc gcggtgcggc agcattttaa aaacaaaact
     4321 ggaaaaagat gtatgtgact attgtggttg cacggaggca atttctatcg atcgggacga
     4381 gcgacacagg ctcttagaga gggagtttga tgggtcggtt agtgataact gggaggaact
     4441 cgacacttat agagaatatc tggcaaaaaa atatgctttc ccagatgaac gctttgatcg
     4501 gaaactgtat gatgaaagac tagaagcgga tgcaaaagaa tacgagcaga tgttgaaaga
     4561 acgggagatt gccaccgcca agttggaata tatgaaagcg catcccaaat gcccggcctg
     4621 tgggtccggc aatgtgcgtg agggcgtgac aagcgggggc tttgacgcga ggggggcgtc
     4681 gatcgggggc tttttcgcgg gcacgaccgg ggccctggta ggcggtatgt ccggcggtga
     4741 agaggtgttt tccctctgtc tcgactgcgg ccaccgctgg gatgtgcgca aaatctggta
     4801 aatctttctt gccccgcgcc cgcgcgggcg cgggggtagg ggataatgag cgtttgataa
     4861 ccggcttcag gctttccctg acgatcagac tgtacttctt ggcattgcga cgcccggccc
     4921 ttccagtcgt tgaacagccg cccggtgacg tccaggggcc ctaccccctg cttttttgac
     4981 gccccttggc gcggctgctg ctgatcgcgc attgcatcgc cacttagcac cgctcacgcg
     5041 gctttttctc agcatatggc atccctgcat tttttctgcc tttcggcgcc gtcccgttcc
     5101 attactgtac cgttttggct gcagggcttt agcgtttccc agcaattgga gcccttgttt
     5161 ttgcaaaagc gttgcatcct atgccgccat ggcttcgcaa ttggcataag agggcataat
     5221 ggctttaccg gcgcttttgt cgcccgacag cagggcggcc aggctgttgc tgcgcaatga
     5281 tttcagcttc agctccagct tgtccagctg ttcaaacgcg gctttcatcc ctttggatgc
     5341 ggcccccgtc ccgcctgtct gggcgagggc tttttccgag accgctttcg cctgggcggc
     5401 cagctttgtg gcgtccagcg ccgcctccag ctttaccttg ggcaggtcca gccgcccgtt
     5461 cacgacacgt tgcaggcccg cgttgatctg cgccgccgtg gcggcctcgt ccacgccgaa
     5521 cggcagcttg accgggtggc ggtcgaaata gtccttgatc ggcttcatcg cggcctcggc
     5581 gtccttggcg gtgccgctga aaaccagctt tgctttcagc tcataggtct cagccatttg
     5641 cccacctctc ttttttatct tgaaacattt taaaaactcc ttttaaatcc aaaaaagaag
     5701 ggcggcccgc cctcctttgc cgctagtctg aaatggtctc ccactccagc atttccttgt
     5761 agatgcgttc cacatagtgg ttgccgccca gctttttgta ctgcgcgtac atatccacga
     5821 tattttcttt ttcccggatg gaaaaggctt tttccggcag gttcttttca tagatatggt
     5881 tgatggtgtt gcgcagctgg aggatcgcgg tctcgttttg cagcgccatc tgcttttcaa
     5941 tgtccgcttt ccaccttgcg tccgcgtcca gctgccgctg cagcagatcg tgcgtctttt
     6001 gcaaaagctc ctccgactcc gccgagcggg tgatctttct gatctcgctt ttcacccgcg
     6061 ttttgagccg ggtgcgcagc ggcttgacca gcgccaggca aaagccgaaa agggtggtca
     6121 gggcccccac cagggcggcg gcgtttttca gctggtcggc ggtcatcatt tcacgtttgc
     6181 tcccctcctt tttgctcttt ggcaccctag ggcgaggcgg cccctgccgg tttttgcccc
     6241 ggcagagccc cggcgcgctg cgcccgccgc gcgcttcagc gcccgtttcc ctttccctcc
     6301 gccgacatcg ctgcgcccgg ccctgcggac ggcccgtggg ggaacgtcac cccgttggcg
     6361 ccctgccctt tggcgggggc ttttttcttt tcggcggttt tcccggccgc ctttacagcg
     6421 cccggtgagg agagc
//
LOCUS       DBMDYA010000060         6359 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3110_length_6359_cov_1.419303, whole genome shotgun sequence.
ACCESSION   DBMDYA010000060 DBMDYA010000000
VERSION     DBMDYA010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_6359_cov_1.419303"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="ACZPFK_02455"
     CDS             <1..964
                     /locus_tag="ACZPFK_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1405205.1"
                     /translation="TSAGYNSYLEDPLKPLINRAFTGLYRPGSTFKCAVGAAGLITGA
                     ITPEYTYRCGGTYTYWAPHFMPNCTHTHGNENVQGALRDSCNIFFYDLGRRLGLDTFN
                     NMASALGLGQKTGLEVGESGGNLSTPEYHETMTGEDWQQGNVVQAAIGQMDTQVTPVQ
                     LATYGAAIANSGTRYRTHLVAAIRSYNFDEVIEETPVTVEARVEGDPAAVEAAFNEVE
                     QGMLLASKVGTARQFLADYQYHIASKTGTAENGKLDKFGKKEYNATIVAYGPTEEPEL
                     AVSAVAEVAGNGYQLARAVRDVFDAYYVDKNQNSAPVASGTLLP"
     gene            1141..>1838
                     /locus_tag="ACZPFK_02460"
     CDS             1141..>1838
                     /locus_tag="ACZPFK_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1405206.1"
                     /translation="MVASGKGGTGKSTLSVYLGARLAAHGARVLLVELDSGLRSVDII
                     AGVCGKTVYDIEDILSGRCEADKAVVESPLYPGLSVVSAPYSGGEIRAGALRVLAGKM
                     SGCFDIVLLDTAAGLGAPFLAAKSVAGMGLVVITPDTVTMRDGRIVADELFAAGLPSV
                     RLVINRVNVGPFAKNPVKNLDECIDTVGAQLIGVVPQSAELAAAAACGEPLPENTLAF
                     AALDNIARRKGEGIL"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1961..2365
                     /gene="mgsA"
                     /locus_tag="ACZPFK_02465"
     CDS             1961..2365
                     /gene="mgsA"
                     /locus_tag="ACZPFK_02465"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR1405207.1"
                     /translation="MNIALIAHDSKKELMVQFCIAYCRVLSQHNLVATGTTGKLVGEA
                     TGLSIQRFLPGTHGGDQQIAARIACNEIDLLLFFRDPISAKPHEPNELNLLRLCDVHN
                     IPVATNIATAEVLIHGLERGDLDWRNIVNPKM"
     gene            2642..3445
                     /locus_tag="ACZPFK_02470"
     CDS             2642..3445
                     /locus_tag="ACZPFK_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405208.1"
                     /translation="MERVKKFAELGTPAELSAFLERRAGAHRSLSQYTTLTVLKKIVA
                     GGAWHFCRPDGMNDGEEYRRIPARFRQSLYFACFTFMNSESMAMWGLYSIPREEGVRI
                     TFPGKVWREWLSGAHTVREAGGGRAVEDVPVLARDVAYMGRDRVGLYWNASSNKRLLQ
                     KHTVRALHEALPAGVKHYAWKYEQECRAFLAAPDLPDCGALALPLPQRCLEQLQVTYG
                     PYVPEAAVAETDCFLLEQGLRRGSRSEFTGLVDLRDLCGLCVHRFQRRG"
     gene            3650..4924
                     /gene="hisS"
                     /locus_tag="ACZPFK_02475"
     CDS             3650..4924
                     /gene="hisS"
                     /locus_tag="ACZPFK_02475"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1405209.1"
                     /translation="MEKITAQKGTKDVLPAESYRWHHLESVLKSTVAAFGYKEIRLPT
                     FEATELFSRGVGETTDVVGKEMYTFNDKGGRSITLRPEGTANTVRAILEHGLLAEALP
                     VRAFYLQSCFRYEKPQSGRLREFHQLGIEMFGAAAPEADVQVVATAAACIANVGIRSV
                     QLQLNSIGCPNCRPRYHEALKSYFRGHLDELCDTCRDRLDRNPLRILDCKCPSCAAVA
                     KDAPLMREFLCGECKDHFAAVKRGLDNIGIPYMENPTIVRGLDYYTRTVFEFVHTGAG
                     AQGVVCGGGRYDGLVEQLGGKPTPGIGFGMGLERLLMVLEAERVPFPPDRVPDLYLCS
                     MGEEARLLAERVAAGLRAKGLWAETDVMGRGLKPQMKYANKTGARYTVVLGEDEVATG
                     KARLKRMGDGAEAETTVERLAQSGPAEWEQLF"
     gene            5036..>6359
                     /gene="aspS"
                     /locus_tag="ACZPFK_02480"
     CDS             5036..>6359
                     /gene="aspS"
                     /locus_tag="ACZPFK_02480"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1405210.1"
                     /translation="MALERMGTLRRTDYCGELTPARVGDTVVVAGSIAKSRDLGGVIF
                     ADLRDTTGILQLAFDDGTDKGVFAKAASLKSEYVVMAKGLLRRRESVNKTLPTGEIEL
                     YVAELKILSEAETPPFEVRDEVKVKDELRLKYRYLDLRRESMHQPIVLRSKIAQIIRN
                     YYCDNHFCEIETPMLIKSTPEGARDYLVPSRVQPGHFYALPQSPQLYKQILMLSGFDR
                     YFQIVRCFRDEDLRADRQPEFTQVDMEMSFVEEEDIETMNEGLIKKLFKEILDVEVET
                     PFLRMPYAEAMARFGSDKPDTRFGLELQDVSDTVRGCGFGVFTSALKAGGSVRCINAK
                     GLADRMSRKEIDKLVEVVKTYGAKGLAYTRCTAESETSSFEKFLTPEEKGALRAAAGV
                     ETGDVLLVVADAKNDVVFAALGALRLEIAKKYGLIDPDKFNFLWVTDFP"
BASE COUNT         1135 a         2011 c         2203 g         1000 t
ORIGIN      
        1 gacctcggcg ggctacaact cctatctgga ggacccgctc aagccgctga tcaaccgcgc
       61 gttcaccggc ctctaccgcc ccggctccac gttcaagtgc gcggtgggcg cggcgggcct
      121 gatcaccggc gccatcacgc cggagtacac ctaccgctgc ggcggcacct acacctactg
      181 ggcacctcat tttatgccca actgcaccca tacccacggc aacgagaacg tgcagggcgc
      241 cctgcgggat tcctgcaaca tcttcttcta cgacctgggg cggcggttgg gccttgacac
      301 gttcaacaac atggcctccg cgctgggcct gggccagaag accggcctcg aggtgggcga
      361 gtcgggcggc aacctgtcca cgccggaata ccacgagacc atgacagggg aggactggca
      421 gcagggcaac gtggtgcagg cggccatcgg ccagatggac acccaggtga ccccggtgca
      481 gctggccacc tacggggcgg cgatcgccaa cagcggcacc cgctaccgca cccatctggt
      541 ggcggcgatc cgcagctaca acttcgacga ggtgatcgag gagaccccgg tcaccgtgga
      601 ggcccgggtg gagggggatc ccgccgccgt ggaggccgct ttcaacgagg tggagcaggg
      661 catgctgctg gccagcaagg tgggcaccgc cagacagttt ttggccgatt accagtatca
      721 tatcgccagc aagaccggca cggcggagaa cggaaaactg gataagttcg gcaaaaagga
      781 gtacaacgcc accatcgtgg cctacggccc caccgaggag ccggagctgg ccgtcagcgc
      841 cgtggccgag gtggcgggca acggctacca gctggcccgc gcggtgcggg acgtctttga
      901 cgcctattat gtcgacaaaa accagaacag cgccccggtg gcaagcggga ccctgctgcc
      961 ctgagcaaaa aacgcttcat aaaaccggca atgtgacgga cttttggcac aattttattg
     1021 ttgtactgtg gattttggta tgatataatg aatctgtatc gaaatcgtgt catttgtgac
     1081 actttttcga actgcagaga aaacgcggtg aaggggcgga tacagacggg aaaggtcctc
     1141 atggtagcct ccggcaaggg cggcaccggc aaaagcacgc tgtccgtcta tctgggggcg
     1201 cggctcgccg cccacggcgc ccgggtgctg ctggtggagc tggactccgg cctgcgcagc
     1261 gtggacatca tcgcgggggt ctgcggcaag acggtctatg acatcgagga catcctctcc
     1321 ggccgctgcg aggcggacaa ggcggtggtg gaaagcccgc tctatccggg cctctcagtg
     1381 gtcagcgccc cctacagcgg cggcgagatc cgcgccgggg cgctgcgggt gctggcgggc
     1441 aagatgtccg gatgttttga catcgtgctg ctggacaccg ccgccgggct gggcgcgcct
     1501 tttttggccg ccaagtcggt ggcgggcatg ggcctggtgg tcatcacgcc cgacacggtc
     1561 acgatgcggg acggccgcat cgtcgccgac gagctgttcg ccgccgggct gccctcggtg
     1621 cggctggtca tcaaccgggt caacgtgggg ccctttgcca aaaacccggt gaagaacctg
     1681 gacgagtgca tcgacacggt gggcgcgcag ctcatcgggg tggtgcccca gagcgccgag
     1741 ctggccgcgg ccgcggcctg cggcgagccc ctgcccgaga ataccctcgc ctttgcggcg
     1801 ctggacaata tcgcccggcg caaaggcgag ggtattctnn nnnnnnnngc ccggcgcatc
     1861 ctcggcgagg aggtgccgct ggtggtgcgc taggcggcag tgatttgcgt ttgacgcggc
     1921 cccgtgccgc gatcctcatc atataacgga ggtagagcag atgaatatcg ccctcattgc
     1981 gcatgattcc aaaaaagaac tgatggtgca gttctgcatc gcttattgca gggtgctcag
     2041 ccaacacaat ctcgtggcca ccggcaccac cggcaagctg gtgggcgagg ccacgggcct
     2101 gtcgatccag cgctttctgc cgggcaccca cggcggcgac cagcagatcg cggcgcgcat
     2161 cgcctgcaac gagatcgacc tgctgctctt tttccgcgac cccatctcgg caaagccgca
     2221 cgagcccaac gagctgaacc tgctgcggct gtgcgacgtg cacaacatcc cggtggccac
     2281 caacatcgcc acggcggagg tgctgatcca cggcctggag cgcggcgacc tggattggcg
     2341 caatatcgtg aaccccaaga tgtgatcccc tccgcgcggc cccgcagggg cggcggagac
     2401 gggacggcaa gcctccctgc acagggctct gtgcggggag gcttttttat ccctgggcgc
     2461 gcggtgcgcc gcccctgccg cgcgtgggac aggccctgcc gtgggacggc cgcggcagcg
     2521 tttttgcgtg caggggggcg ctgagccccg gcggcagagg ggccaccccc gcgcgcgaaa
     2581 cacggctttt caggcgcggg gcgggacagg gcgcggcggc acaggagcgg aggaggataa
     2641 aatggagagg gtaaaaaaat ttgcagagct ggggacgccg gcggagctgt cggccttttt
     2701 ggagaggcgg gccggggcgc accgctccct gagccagtac accaccctca cggtgctgaa
     2761 aaagatcgtg gccggcggcg cctggcactt ctgccgcccg gacggcatga acgacggcga
     2821 ggagtaccgg cgcatcccgg cccgcttccg gcagagcctt tattttgcct gcttcacctt
     2881 tatgaacagc gagagcatgg ccatgtgggg gctgtactcg atcccacggg aggagggcgt
     2941 gcgcatcacg ttccccggca aggtctggcg ggaatggctg tccggcgccc acacggtgcg
     3001 ggaggctggc ggcggccgcg ctgtggagga tgtccccgtt cttgcccgcg acgtggccta
     3061 tatgggccgg gaccgggtgg gcctttactg gaacgcctcg tccaacaaaa ggctgctgca
     3121 aaagcacacc gtccgcgcgc tgcacgaggc tttgccggcg ggtgtcaagc attatgcctg
     3181 gaagtatgag caggaatgcc gggcctttct cgccgcgccg gacctgccgg actgcggcgc
     3241 cctggccctg cctttgcccc agcgctgcct ggagcagctg caggtcacct acggccccta
     3301 tgtgccggag gccgcggtgg ctgagacgga ctgctttctg ctggagcagg ggctgcgccg
     3361 gggcagccgc agcgagttca cgggcctggt ggacctgcgg gacctctgcg ggctgtgcgt
     3421 ccaccgcttt cagcgcaggg gctgaggcgg gggcggcccg gggccgcccc cgctgggggc
     3481 cgcgccacaa acggcgctgc aaagccaaaa acattgacaa tcaccggccg ctgcggtaca
     3541 ctaaaacggt attgacaacg agagggggac ccccctgcgg gccggccggc ccgcccgcgc
     3601 ttttggggcg cggggcgcct tgggcgcagg ggcggggaca ggaggaagta tggaaaagat
     3661 caccgcacaa aagggcacca aggacgtgct gcccgccgag agttatcgct ggcaccacct
     3721 ggaatcggtg ctgaaaagca ccgtcgcggc ctttggctat aaggagatcc gtctgcccac
     3781 ctttgaggcc accgagctgt tcagccgggg cgtgggcgag accaccgacg tggtgggcaa
     3841 ggagatgtac accttcaacg acaagggcgg gcgcagcatc accctgcggc cggagggcac
     3901 cgccaacacg gtgcgggcca tcctggagca cggcctgctg gccgaggcgc tgccggtgcg
     3961 ggcgttttat ctgcagagct gcttccggta tgaaaagccc cagtccggcc gcctgcgcga
     4021 gttccaccag ctgggcatcg agatgttcgg cgcggcggcc cccgaggcgg acgtgcaggt
     4081 ggtggccacc gcggccgcct gcatcgccaa tgtgggcatc cgctcggtgc agctgcagct
     4141 caactccatc ggctgtccca actgccgccc ccgctatcac gaggcgctga agagctattt
     4201 ccgcggccac ctggacgagc tctgcgacac ctgcagggac cggctggacc gcaacccgct
     4261 gcgcattctg gactgcaagt gcccctcctg cgcggcggtg gccaaggacg cgccgctgat
     4321 gcgcgaattt ttgtgcggcg agtgcaaaga tcactttgcc gcggtgaagc ggggcctgga
     4381 caacatcggc atcccctata tggaaaaccc caccatcgtg cgcgggctgg actattacac
     4441 ccgcaccgtt tttgagttcg tccacaccgg cgcgggcgcg cagggcgtgg tctgcggcgg
     4501 cggccgctac gacggcctgg tggagcagct gggcggcaag cccaccccgg gcatcggctt
     4561 cggcatgggg ctggagcggc tgctgatggt gctggaggcg gagagggtgc ctttcccccc
     4621 cgaccgggtg cccgacctgt acctgtgcag catgggcgag gaggcgcggc tgctggccga
     4681 gcgggtggcc gccgggctgc gggcaaaggg cctttgggcc gagaccgacg tgatgggccg
     4741 cggcctcaag ccgcagatga aatacgccaa caagaccggc gcccgctaca cggtggtgct
     4801 gggcgaggac gaggtcgcca cgggcaaggc ccggctcaag cggatgggcg acggcgccga
     4861 ggcggagacc acagtggagc ggttggcgca gagcggcccc gcagagtggg aacagctgtt
     4921 ttgaccaggg ccgggcgccg cccggctctt tgcatcccct ggggcgccgt gcggctgcgc
     4981 ggccccagga ctaaccccgg cctgccgggg gatcaatgag aggaacggac aagttatggc
     5041 actggaaaga atgggaaccc tgcgccgcac cgactactgc ggcgagctca ccccggcgcg
     5101 ggtgggcgat acggtggtgg tggccggctc catcgccaag agccgcgacc tgggcggcgt
     5161 gatctttgcc gacctgcggg acaccaccgg cattttgcag ctggcgttcg acgacggcac
     5221 cgacaagggg gtgttcgcca aggccgcgtc gctgaaaagc gagtatgtgg tcatggcaaa
     5281 gggcctgctg cgccgccgcg agagcgtcaa caagacgctg cccaccggcg agatcgagct
     5341 ctacgtcgcg gagctgaaaa tcctcagcga ggccgagacc ccgccctttg aggtgcggga
     5401 cgaggtgaag gtcaaggacg agctgcggct gaaataccgc tatctggacc tgcggcgcga
     5461 gagcatgcac cagcctattg tgctgcgctc gaagatcgcc cagatcatcc gcaactacta
     5521 ctgcgacaac cacttctgcg agatcgagac cccgatgctg atcaaatcca cccccgaggg
     5581 cgcgcgggac tatctggtgc ccagccgggt gcagcccggg cacttttatg cgctgcccca
     5641 gtcgccgcag ctgtacaagc agattttgat gctgtcgggc tttgaccgct atttccagat
     5701 cgtgcgctgc ttccgcgacg aggacctgcg cgccgaccgc cagcccgagt tcacccaggt
     5761 ggatatggag atgtcctttg tggaagagga ggacatcgag accatgaacg aggggctcat
     5821 aaaaaagctt ttcaaggaaa tactggatgt tgaggtcgaa acgcctttcc tgcgcatgcc
     5881 ctacgcggag gccatggcgc gcttcggctc agacaagccg gacacccgct tcggcctgga
     5941 gctgcaggac gtctcggaca cggtgcgcgg ctgcggcttc ggcgtgttca ccagcgcgct
     6001 aaaggcgggg ggcagcgtgc gctgcatcaa cgccaagggc ctggcggaca ggatgtcccg
     6061 caaggagatc gacaagctgg tggaggtggt gaagacctac ggcgccaagg gcctggccta
     6121 tacccgctgc acggccgaga gcgagacctc cagctttgaa aagtttctca ccccggagga
     6181 aaagggcgcg ctgcgcgcgg ccgcgggggt cgagaccggc gacgtgctgc tggtggttgc
     6241 ggacgcgaaa aacgacgtgg tcttcgcggc cctgggcgcg ctgcgcctgg agatcgccaa
     6301 aaagtacggc ctcatcgacc cggacaagtt caacttcctc tgggtcaccg acttcccgt
//
LOCUS       DBMDYA010000061         6347 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3115_length_6347_cov_1.561479, whole genome shotgun sequence.
ACCESSION   DBMDYA010000061 DBMDYA010000000
VERSION     DBMDYA010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_6347_cov_1.561479"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="ACZPFK_02485"
     CDS             <1..286
                     /locus_tag="ACZPFK_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405211.1"
                     /translation="RTAAQNRMQVFLLAVKKRMAESPPFCPAEDTGAALRAGPTRKKR
                     LKYDRRIRDKTIIPPRRAGWPERGVALFGSAGWPAVRPKNKISLRIGFFI"
     gene            complement(279..1622)
                     /locus_tag="ACZPFK_02490"
     CDS             complement(279..1622)
                     /locus_tag="ACZPFK_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405212.1"
                     /translation="MTVCWRGKNKFSKFVFISMLWIFLIFALSGCNTVPLAGSGAPIG
                     ASGPTPTAEPARENLRLISAQGGPIFTGISGTKNGGYEIFAHTDATADIIYYDYQTLQ
                     KIYLSSSPNETHNENSTAYLDNVMGGAVALASEKCLYILKAGSNAIGGGTDLAPYLLR
                     MDLDGSNRERFDIPGQYELDVASAVAADDTGLYIALHVIEDLKTDQSRYMIAKVDFDH
                     KKLEELYRFDPRQSVRIVGTYSYGLILESVKNIKSGTAIQKETLLFNLNSNQAKAIKE
                     WKAGDVGVAYDRDAMYLLKSDDLTLYRSALYPDSAGPEEKVIDCTDLIPDAYTGIALD
                     RAVYDRHLIFQITSEQSGAEPLYLSLTLDGLETREMRLMCKRNGEEQLATIMQETDTH
                     FVVYYGEEAISYLDKGPGGEPYEMTQYIPSYRLIEKTAYWENIPDFMAFEDHVLK"
     gene            complement(1776..3212)
                     /locus_tag="ACZPFK_02495"
     CDS             complement(1776..3212)
                     /locus_tag="ACZPFK_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405213.1"
                     /translation="MKRLAALFCSFLMSLLFCCACSSDSMLSTSTDSAPDKVFSESSG
                     DTSTTEYTLEKDRTVECKQVLNDSTENFRMVSTTDLTAYGSGNEQGFYQIFLNDDNSK
                     NILYVDYATHQQVYLCSQPNCEHDSESCTSWIPPEMGQVNVVTLPDRILLIHNNMTEI
                     TYIEQLNLDGSDKEILYKFEGSVDIANGVAYNGKYIVFKLETYTRDSADNFTNVPSLG
                     AISLEKKEFITLLSLKPGNHASNGQALFLFGTSKTGFILKTISVREDLLAATVGEDSG
                     ENPALAMAHTMYELPFNGDSTREIFSYLQDECRADIYGGDLFLLCENGNSGVMLQMIP
                     DCRGEPQTIVDDFESAAGDLITGITTTTIFPRARVDDYLLFNFESSSGIKENGDIEVI
                     FKRVRVDLKSGMVKENTLSNYYNATNVPVEILGEAGEQLLVQASVNNVASTGKIAGVE
                     RSLGLISKNDFLASRPNYTRITEQRKAA"
     gene            complement(3284..3820)
                     /locus_tag="ACZPFK_02500"
     CDS             complement(3284..3820)
                     /locus_tag="ACZPFK_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405214.1"
                     /translation="MKRFGKKIAAVMLAMAMMMAMAVPAFAKNISTSTNNTQYIVGWN
                     NEGNAFLTGMGNGATVQLSTNTSTQHGWVVSAAPDGNYVIRDVRNGLYINIHRILVGG
                     AYYMCTEYTYESAIQGRDQRVNILNYSSTGYSNVQLAAPQMGGTWYMVADRSSPSTSS
                     DVIWYIYNYGAKAQFCTR"
     gene            4155..4517
                     /locus_tag="ACZPFK_02505"
     CDS             4155..4517
                     /locus_tag="ACZPFK_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405215.1"
                     /translation="MELELDYKAIGTRIRDRRLKLGWSQERLSEAADLTKPHISHIEN
                     GHTKLSLPALVRLAAALGVATDELIFDRAVVTESGFNFELAQLCKGRSSEELAFILSV
                     VETMIVHFQQRGGSGGTV"
     gene            4538..5059
                     /locus_tag="ACZPFK_02510"
     CDS             4538..5059
                     /locus_tag="ACZPFK_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1405216.1"
                     /translation="MLAFAVEDRPLIARIYESKKELLGRYAYSVLKDEQEAADVVQQS
                     VLDFMEAFHRLSHLSEDKLCGYLFSITRNNVYDECKRRNKLFPSEWMIDEADPIDTEE
                     VALTRVGIEDMKKAIRHLPPRYGSYLQMVYLDKLDREVVARALGVKPDSLRMIDVRAK
                     KLLKALLLRGEEV"
     gene            5062..5796
                     /locus_tag="ACZPFK_02515"
     CDS             5062..5796
                     /locus_tag="ACZPFK_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025643.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR1405217.1"
                     /translation="MRSDSEKELERLQEEFLLQSAGVAYEDFLFETFEQVGQDPTAAG
                     LPRERMASIDAAVEAGVKKLRRRDNQKRLRANLLKVAACFVLLLVAVPSVAFSVKASR
                     SGIVNYLIQHFSQFSEIHYGPDLKATAPLGWSSPYYPTWLPEGFSIYQVGFNENADTV
                     WYNVPGEHKVLFSVLKNLSTGTAVNDEDLQGKSLQINGASAELYFNESGTERMLVLPL
                     PRGVVQIRGEISEENIIKIAESIEQK"
     gene            5861..6289
                     /locus_tag="ACZPFK_02520"
     CDS             5861..6289
                     /locus_tag="ACZPFK_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405218.1"
                     /translation="MKKVVVRLSSLALALVFVMALMAPAMAVSNPQVQPRWAALNTLQ
                     CGLTPKSSLFSNARASATVTTADPDCKLSLTLTIQVMSGGEFVDTDKSWSSSGKGSTS
                     VGKDVKLGAGNYRSKAVVTVYSASGAYIETVTGYSADILI"
BASE COUNT         1563 a         1580 c         1566 g         1638 t
ORIGIN      
        1 caggaccgcc gcgcagaaca gaatgcaggt ctttcttttg gcggtaaaaa agcgcatggc
       61 ggaaagcccc cctttttgtc cggcggagga taccggcgcg gctttgcgcg ccgggcccac
      121 acgcaagaaa aggctgaaat acgaccgccg gatcagagat aagacaatta taccaccgcg
      181 ccgggcgggt tggccagagc ggggcgttgc gcttttcggt tcagcaggat ggcctgcggt
      241 gagaccaaaa aacaaaatca gccttagaat cggctttttt atttaaggac gtggtcctca
      301 aaagccataa aatcaggtat attttcccaa taggctgtct tctcgatcaa tcgatagctc
      361 ggtatgtatt gggtcatttc ataaggttcc ccacccggcc ccttatccag atacgaaatt
      421 gcttcctctc cataatatac gacaaaatgg gtatctgtct cctgcatgat cgttgccagc
      481 tgctcttctc catttcgttt acacatcaag cgcatttccc ttgtttccag tccatcgagg
      541 gtcaacgaga gatagagggg ctctgcgccg ctttgttcgc ttgtgatttg aaaaatcaaa
      601 tggcggtcat atacggcgcg gtctaaagcg atgcctgtgt aggcgtcagg tatcaagtcg
      661 gtacagtcga tcactttctc ctccggtccg gccgaatcgg gataaagagc cgacctgtat
      721 aaggtcaggt catcggattt aagcaggtac atggcatccc tgtcataagc gacccctaca
      781 tcgcctgctt tccattcttt tatcgctttc gcctgattgc tgtttaaatt gaacagcagc
      841 gtttcttttt gaatcgcagt tccgcttttg atatttttta cagattcaag gattaggcca
      901 taagagtatg tccccactat tctaaccgac tgccggggat cgaaacgata cagctcttcc
      961 agctttttat ggtcaaaatc aactttggca atcatatacc ggctttgatc cgtttttaaa
     1021 tcttcgatga catggagagc aatatagagc cccgtatcat ctgcagcgac tgcgcttgcg
     1081 acatccaatt catattgacc cggaatgtca aatctttccc tgttgcttcc gtccagatcc
     1141 atgcggagca gataaggggc aaggtccgtc cccccaccta tggcgttgct tcccgctttt
     1201 aaaatatata aacatttctc ggacgccaga gcgacagccc ctcccatcac attgtcaaga
     1261 tatgccgtgc tgttttcatt atgcgtctcg ttgggagaac tggaaagata tattttttgg
     1321 agagtttgat agtcgtagta aatgatatcc gcggtggcat cggtatgggc aaaaatttca
     1381 tagccgccat tttttgtccc cgaaatccct gtaaagattg gcccgccttg ggcagatatc
     1441 agccttaaat tttccctggc gggttctgcg gtgggggtgg gacccgatgc tcctatcggc
     1501 gctccgctgc cggctaatgg gacggtattg catcccgaca gggcaaaaat caaaaatatc
     1561 cagagcatag agatgaaaac gaatttagag aacttgtttt ttcctctcca acacaccgtc
     1621 atgctccttt tctccgcaaa cagttccggc ggatcaaacc gccgccgtgt ccggcgccga
     1681 cggcaaagta cccggatgtc acacaacaga agaactgcct atagcagagt ggaaaacttg
     1741 tctcgacaaa cattttaacc ataatccgca tccgtttacg ccgctttacg ctgttccgtg
     1801 atcctggtgt aattgggccg ggaagccaaa aaatcatttt tactgatcaa ccccagggaa
     1861 cgctcgaccc cggcaatttt ccccgtagaa gcgacgttat ttaccgaggc ttgtaccaga
     1921 agctgttcac ccgcttctcc caaaatctct actgggacat tggtcgcatt atagtagttg
     1981 gagagggtgt tttctttcac cataccgctc ttcagatcta cccgcacccg tttaaagatg
     2041 acctctatat cgccgttttc cttgatgccg ctgctgcttt caaagttaaa caaaagatag
     2101 tcgtcgacac gggcccgggg aaatattgtg gttgtagtaa tccccgttat caaatcgccg
     2161 gctgcacttt caaaatcgtc tacaatggtt tgaggctctc ctcggcagtc gggaatcatt
     2221 tgtagcatta caccagagtt tccattttca cataaaagaa aaagatcacc gccataaatg
     2281 tcagcgcgac attcgtcttg tagatacgaa aatatctctc ttgtagaatc gccattaaaa
     2341 ggcagctcgt acatagtgtg agccatcgcc agggcgggat tttctccaga atcctcccct
     2401 actgtagctg caagtaggtc ttcccgcacc gaaatggtct taagaataaa tccagtttta
     2461 gaagtcccga agagaaagag cgcctgccca ttagaagcgt gattacctgg tttaagactt
     2521 aaaagagtaa taaattcttt tttctccaga gaaattgcgc ctaaggaagg aacatttgta
     2581 aaattgtcag cagagtctcg agtataggtt tccagtttga atacgatata ctttccgttg
     2641 taagcgactc cattggcaat atccacgctt ccctcaaatt tgtataatat ttctttgtca
     2701 gagccgtcta ggttcaactg ttcaatataa gtgatctcgg tcatattatt atggattaat
     2761 aggatgcgat ccggtagagt cacaacattg acctgtccca tttccggcgg aatccaggaa
     2821 gtgcaggact cactatcgtg ttcacagttg ggctgcgaac agagatagac ctgctgatgt
     2881 gttgcatagt cgacatacaa aatatttttg gaattgtcat cattgagaaa aatctgataa
     2941 aatccctgct catttccact tccgtaggcg gtcaaatctg ttgtagaaac cattctgaag
     3001 ttttctgtgg aatcgttcaa aacctgcttg cattcaactg ttcggtcttt ctccaaggta
     3061 tattcggtgg tcgaagtgtc ccctgaactt tcagagaaaa ccttatccgg ggcagaatct
     3121 gtagaggtgg agagcatgga atcagaagaa catgcacaac aaaaaagcaa agacatcaga
     3181 aatgaacaga aaagagccgc caatcgtttc atatttaaat cccctttgca gtacaactaa
     3241 gcatgatgca cttgcgtgca tcatgcttag ttgttatctt tgattatcgg gtgcaaaatt
     3301 gcgctttagc gccgtaattg tagatatacc agatcacgtc actggaagtg gacgggctgc
     3361 tccgatccgc aaccatgtac caagtaccgc ccatttgagg tgcagccagc tgtacattgc
     3421 tatatcccgt ggaagaatag tttaaaatat tcacacgttg atctcttccc tggatggcac
     3481 tttcatacgt atactcagta cacatgtaat aagccccgcc taccaaaatc cgatggatat
     3541 tgatataaag gccattccgt acatctcgaa taacatagtt tccatcaggc gcagccgaaa
     3601 caacccagcc atgttgagta ctggtgttgg tactcaactg aacagttgcc ccgtttccca
     3661 tgcctgtgag gaaagcattt ccttcgttgt tccatccgac aatgtattgt gtattattgg
     3721 tggaagtact gatgtttttc gcaaacgccg gcacagccat cgccatcatc attgccatgg
     3781 ccagcatgac cgctgcgatc ttttttccaa accgtttcaa ctttttcact ccgtttcttt
     3841 ttatattgtt tattgcttca ccggcatcgc gcgccgaatg ccggtcaagg cctgctctgg
     3901 accaacatct ttgctactgt cctcattata gaacttgtga aagaaaagtg aaattcgctt
     3961 ctttcatcaa cttttcgaca aaaattttct ctaatctgta aaaacctgtt ttccgcaaca
     4021 ggtcttttaa aaaagttttc aggccaaaac agcaacttaa gactttataa tttaatatat
     4081 tgaccgcaca atgcatttta accccataag tttgcccata caatataatc aaaataattt
     4141 gtgagggcac atacatggag ttggagctgg attataaagc aatagggacg cgcatccgcg
     4201 accggcggct gaagctgggt tggagccagg agcgtctgtc ggaagcggcg gacctgacaa
     4261 agccccacat cagccatatc gaaaacggcc acaccaaact cagcctgccg gcgctggtcc
     4321 gtcttgccgc cgctttgggc gtcgcgacgg acgaattgat ctttgaccgc gccgtcgtga
     4381 ctgagtccgg cttcaatttt gaacttgccc agttgtgcaa ggggcgcagc tcggaggaac
     4441 ttgcctttat tctatccgtc gtcgagacga tgatcgtcca ctttcagcaa aggggcggca
     4501 gcggcggcac ggtgtgacga gaggaggcgg aggcgctatg ctcgcatttg ctgttgagga
     4561 caggcccttg atcgcaagga tctacgaatc aaaaaaagag cttctcggca ggtatgccta
     4621 ctccgtcctg aaagacgaac aggaggccgc cgacgttgtg cagcagtcgg tgttggattt
     4681 catggaggct tttcaccggc tgtcgcatct ctctgaggac aagctctgcg gatacctgtt
     4741 ttccatcacc cgcaacaatg tctacgacga gtgcaaaagg cgcaacaagc ttttcccttc
     4801 cgagtggatg atcgacgagg ccgatcccat cgacacggag gaggtcgccc tgacgcgggt
     4861 gggcatcgag gatatgaaaa aggcgatccg ccatctgccg ccccgctatg ggtcttatct
     4921 ccagatggtc tatctggaca agctggaccg tgaggtggtg gccagggcgc tgggcgtcaa
     4981 gccggacagc ctgagaatga tcgacgtgcg cgccaaaaag ttgctcaagg ccctgttgct
     5041 gcggggagag gaggtgtgac gatgcgctcg gacagcgaaa aagaactgga acgtttacag
     5101 gaagaatttc ttttgcaatc cgccggggtg gcctacgagg attttctgtt tgagaccttt
     5161 gaacaggtcg ggcaggaccc cactgccgcc ggcttgcccc gcgaacggat ggcgagcatc
     5221 gacgccgccg tggaggcggg ggtgaaaaaa ctgcggcgcc gcgacaatca aaagcgcctg
     5281 cgggcgaatt tgctgaaagt ggccgcctgt tttgtgctgc tgctggttgc agtgcccagc
     5341 gtcgctttct cggtcaaggc ctcccgttcc ggcatcgtca attatttaat tcagcatttt
     5401 tctcagttca gtgagattca ctacggcccc gacctgaaag cgaccgcgcc gctgggctgg
     5461 tcatcgccct attatcccac ctggttgccg gaggggtttt ctatttacca agtcggattc
     5521 aatgagaatg cagacactgt ttggtataac gtcccagggg aacataaggt attgttttct
     5581 gttttgaaga atctctcaac gggtacggca gtcaatgacg aggatttgca aggcaagtct
     5641 ttgcagatca acggcgcttc ggcggagctc tatttcaatg agagcggcac cgagcgcatg
     5701 ttggtcctgc ccttgccccg gggggtcgtt caaataaggg gcgaaatctc ggaagaaaat
     5761 atcatcaaaa tcgcggaaag catcgagcaa aaataatttt gtgttgcgcg gcctccctct
     5821 tatcagttgt atggatagta aaaaataggg aggctttgcc atgaaaaaag ttgttgtccg
     5881 tctgtcgtct cttgctctgg cgctggtctt tgtgatggct ctgatggcgc ccgccatggc
     5941 cgtgtcaaat ccgcaggtgc agccgcgatg ggcagcgctg aatacgctgc agtgcgggtt
     6001 aaccccgaag agtagcctgt tcagtaatgc ccgagcctct gccactgtca caactgctga
     6061 tccggactgt aagctgtctc tcaccctgac gattcaggtc atgagcgggg gcgagtttgt
     6121 ggacacggac aagtcctggt cctcgtcggg aaaagggtcc acaagcgtcg gcaaggatgt
     6181 gaagctgggg gcgggcaatt accgctcaaa ggcggtggtg acggtgtact ccgcttccgg
     6241 ggcctatatt gaaaccgtca ccggttattc cgcggatatt ctgatctgac aaaagcgaag
     6301 cggcctgcaa acgccgcttg ggatgagagc cggggtggaa gcagttt
//
LOCUS       DBMDYA010000062         6330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3130_length_6330_cov_1.433617, whole genome shotgun sequence.
ACCESSION   DBMDYA010000062 DBMDYA010000000
VERSION     DBMDYA010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3130_length_6330_cov_1.433617"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="ACZPFK_02525"
     CDS             complement(<1..1484)
                     /locus_tag="ACZPFK_02525"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1405219.1"
                     /translation="MDAVIYYGGPILTMEPGPDPEAVLVREGRIEAVGPLEKCLSLAP
                     RARRFCLDGSALLPAFLDPHSHVTSLASTLGLCDLSAAKSFDEIVLALRGFLAANPPA
                     PGGWISGFGYDHNFLREKQHPTRQLLDLVSADIPILISNASGHMGVANSAALRAMGIT
                     ADTPDPEGGLIGREPGSREPGSREPNGYLEETAFTSTGSRAPRPTMEQRVAQLCRAQE
                     IYFSHGITTIQDGLTRDADWALLQTAADQGRLQADIVSYIDLQQCRGLAQDNPRLKQY
                     RGHLKLGGYKIFLDGSPQGRTAWMSQPYQGADDGYRGYPIHSDAAVRDFMAAALEDDV
                     QILAHCNGDAAAQQMLDAYAAAVKKTGRGGIRPVMIHAQLLRADQLPQLAELGMFASF
                     FVAHTWQWGDIHIENFGMPRAARISPVGSALRAGVPYTFHQDTPVLPPDMIDTLFCAV
                     NRVTRSGVQLEQSERIGVLEALRGVTVNAAAQYFEENEKGTLAPG"
     gene            complement(1534..2205)
                     /locus_tag="ACZPFK_02530"
     CDS             complement(1534..2205)
                     /locus_tag="ACZPFK_02530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1405220.1"
                     /translation="MTKLTTVVWDWNGTLLDDVQVSYRTVNRVLAQNGYAPIPDLETY
                     RAKFCFPIIQYYRNVGFDFERTPFETVAQEYMDLYHPAAEGCALQEGAEAVLVALREA
                     GLRQVLLSASLQAHLELQVARTPIRTYFDRLLGIGDIYAASKQELARAFLREAGLDPA
                     SVLFVGDSAHDFEVAQGCGCPCVLFSGGHQPHAVLAATGSPVIAALSELPPLLSSSFL
                     KASPL"
     gene            complement(2227..3141)
                     /locus_tag="ACZPFK_02535"
     CDS             complement(2227..3141)
                     /locus_tag="ACZPFK_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1405221.1"
                     /translation="MIYHIKDGRFVPARDGEEGLAAGPEFGLFPLAQAQQVFDRLAID
                     ARFLPELLSGRAAKFESHDGFDLIRLSVLNGKKIALSGHPVVLYLSQGRLLAFSDSER
                     MLRELASYAEKAEEVAFDRVLYFLFKRLTEEDSGRLDGIEAEILQLEDRLLEGEHAPE
                     CARQIAALRRKLFTLKSYYEQFYNILDGIMENENGLFGREALRYFKIFSGKLDRLGRQ
                     TGNLRDYITQVRESYQAQVDIELNKIMKLFTVITAVISPLTLIAGWYGMNFSMPEYHW
                     ALGYPAVILLSAAVVGLCVYYFKRHRWF"
     gene            complement(3138..3554)
                     /locus_tag="ACZPFK_02540"
     CDS             complement(3138..3554)
                     /locus_tag="ACZPFK_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024060.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1405222.1"
                     /translation="MKARIQTGTILLYSYEDGPERTALEQLFRGARAAVRFAGPDAGG
                     KTLGALLGLPGYPPSGAPQQQAPAEKALVFYAFSDPALDRLLGDLRGRGLALDALKAV
                     VTAHNRGWTLTALLAELRRERESFAKGQGGQGDGAE"
     gene            complement(3841..5010)
                     /gene="rlmD"
                     /locus_tag="ACZPFK_02545"
     CDS             complement(3841..5010)
                     /gene="rlmD"
                     /locus_tag="ACZPFK_02545"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1405223.1"
                     /translation="MTKTPDCPAKKQRCGGCPLLALPYEEQLRQKQELVGYFLGRFCA
                     PDPILSMEVPFHYRNKAIATFAPGPKGKPVCGIYESHTHRVVPVRDCLLQDSRINEVV
                     EAALSAAIRCRLSAFDEDRGTGVLRHMVVRRGAHTGEVSVTVVTPSPFFPGSRDFVRA
                     LRASCPDVATVVQNINPRRTSAVLGSAQKVLYGKGFIEDELCGLRFSVAARAFYQVNP
                     LQTEQLYAAALALAGLAGQERVIDAYCGIGTIALAAAGRARAVLGVELNAEAVRSAVR
                     NARRNGIGNAEFLCGDAGELMTRMAEDGERADLVFFDPPREGASEAFLEALVRLGPQK
                     AVYISCNPETQARDLDYLTGRGYAARRIQPVDMFPHTRHVETVCLLEKRRSALSK"
     gene            complement(5013..5486)
                     /locus_tag="ACZPFK_02550"
     CDS             complement(5013..5486)
                     /locus_tag="ACZPFK_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIR1405224.1"
                     /translation="MKLLFKQRFFSWLDSYDIYDENGNTVYTVEGKLSFGHCLDILDA
                     SGRQLGTVKEEVLTFLPRFRIYAQGQCLGEIRKEFTFFKPAFQLDCNGWRVEGDIFSW
                     DYEITDAAGEPVAHLSKELLRLTDTYVLEFPDPENALLVLMIALAIDAAKCSAGD"
     gene            complement(5665..>6002)
                     /locus_tag="ACZPFK_02555"
     CDS             complement(5665..>6002)
                     /locus_tag="ACZPFK_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405225.1"
                     /translation="EAGVKPEKGYRSMSTPHIVYPGQVDSFSYLNIGFFDNETEAENF
                     RKYMMCKLPRFMMRATYSSAHISQSNFIFVPKMDYTRSWTDRELYEYFDLSNKEIKLV
                     ESTMRPMTE"
     assembly_gap    6003..6012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6013..>6330)
                     /locus_tag="ACZPFK_02560"
     CDS             complement(<6013..>6330)
                     /locus_tag="ACZPFK_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405226.1"
                     /translation="NQDTLKRSLNQYGNLFIRSNKAISIIEKIRTKADFFVDEMVSAI
                     DTFGIPSKVRGHEKYIEGDILLVHSDGANSSKTSYISRDVVTKNAHLIDKYKIRISIL
                     VPQN"
BASE COUNT         1216 a         1916 c         1957 g         1231 t
ORIGIN      
        1 ccgggggcga gggtgccctt ttcgttttct tcaaagtact gggccgcggc gttgaccgtc
       61 acgccgcgca gcgcctccaa aacgccgatc ctttcgctct gctccagctg cacgccgctg
      121 cgggtcaccc ggttgacggc gcagaacagc gtgtcgatca tatccggcgg cagcacgggg
      181 gtgtcctgat ggaacgtgta gggaacccct gcccgcaggg cactgcccac cgggctgatg
      241 cgggcggcgc ggggcatgcc gaaattttca atgtggatgt ccccccactg ccaggtgtgg
      301 gcgacgaaaa aggaggcgaa catgcccagc tccgccagct gcggcagctg gtccgcccgc
      361 agcagctggg cgtggatcat caccggacgg atgccgccgc gcccggtctt ttttacagcg
      421 gcggcatagg cgtccagcat ctgctgggcc gcggcgtcgc cgttgcagtg ggcgaggatc
      481 tggacgtcgt cctccagcgc ggcggccata aaatcccgca ctgcggcgtc ggagtggatc
      541 gggtagccgc ggtagccgtc gtctgcgccc tgatagggct ggctcatcca ggcggtgcgg
      601 ccctggggcg agccgtcgag aaagatttta tagccgccca gcttgagatg gccgcgatac
      661 tgcttgaggc gggggttgtc ctgtgccagg ccccggcact gctgcagatc gatatagctg
      721 acgatgtcgg cctgcaggcg gccctggtcc gcggccgtct gcagcagggc ccagtcggcg
      781 tcgcgggtca gcccgtcctg gatggtggtg atgccgtgag agaagtagat ctcctgcgcc
      841 cggcagagct gggcgacgcg ctgctccatg gtgggccggg gcgcgcggga gccggtggag
      901 gtaaaagcgg tctcctccag atagccgttc ggttcgcggc tgcccggctc gcggctgccc
      961 ggctcgcggc cgatcagacc cccctcgggg tcgggcgtgt cggcggtgat ccccatcgcg
     1021 cgcagggcgg cgctgttggc gacccccata tggccgctgg cgttggagat gaggatgggg
     1081 atatcggcgc tgaccagatc cagcagctgc cgcgtggggt gctgcttttc ccgcaaaaag
     1141 ttgtggtcgt agccaaagcc gctgatccag ccgccggggg cggggggatt ggcggccaga
     1201 aagccgcgca gcgcaaggac gatctcgtca aagcttttcg ccgcgctcag gtcgcagagg
     1261 cctagggtgc tggccagcga ggtgacgtgg ctgtgcgggt cgagaaaggc gggcagcagc
     1321 gcgctcccgt ccagacagaa ccgccgggcc cggggggcca gggagaggca cttttccagc
     1381 gggcccaccg cttcgatacg gccctcccgc accagcacag cttccgggtc ggggcccggc
     1441 tccatggtga gaatggggcc tccgtaataa atgacagcgt ccaaatgtaa gacttccttt
     1501 ccggttttat gggcagccgc agagccgccc gggtcagagc ggtgaagctt tcaaaaaaga
     1561 cgaggataaa agcggcggca gctcgctcag cgccgcaatt accgggctgc cggtggcggc
     1621 cagcacggcg tggggctggt gcccgccgct gaacagcacg caggggcagc cgcagccctg
     1681 cgccacctca aaatcgtgcg cgctgtcccc gacaaagagc accgacgcgg ggtcgaggcc
     1741 tgcctcgcgg agaaaggcgc gcgccagctc ttgcttgctt gcggcgtaga tgtcgccgat
     1801 gcccagcagc cggtcgaaat aagtcctgat cggggtgcgg gcgacctgca gttccaggtg
     1861 ggcctgaaga gaggccgaga gcagtacctg ccgcagcccc gcctcccgca gggcgacgag
     1921 gacggcctct gccccctctt gcagggcaca gccctccgcg gctggatgat agaggtccat
     1981 gtattcctgc gccaccgtct cgaacggggt gcgctcaaaa tcgaacccca cattgcggta
     2041 atattggatg atggggaaac agaattttgc ccggtaggtt tccaggtcgg ggatgggggc
     2101 atagccgttt tgggcgagca ctcggttgac ggtgcggtaa gagacctgca cgtcgtccag
     2161 cagggtgccg ttccagtccc acaccactgt agtgagcttg gtcaagaggc gctcctttca
     2221 tgccggtcag aaccagcgat gtcttttgaa gtaataaacg cacagcccca ccacggcggc
     2281 gctcagcagg atgacggcgg ggtaccccag agcccagtgg tattcaggca tcgaaaaatt
     2341 catgccgtac cagccggcga tcagggtgag aggggagatc acggcggtga tgacggtgaa
     2401 cagcttcatg atcttgttga gctcgatgtc cacctgggcc tgataggact cccgcacctg
     2461 ggtgatatag tcgcgcaggt tgccggtttg gcggcccagc cggtccagct tgccggaaaa
     2521 gattttgaaa tagcgcagcg cctcccgccc gaacaggccg ttttcatttt ccatgatacc
     2581 gtcaaggatg ttatagaact gttcatagta gctcttgagg gtaaagagct tgcggcgcag
     2641 ggccgcgatt tgccgcgcgc attcgggcgc gtgctcgccc tccagcagcc ggtcctccag
     2701 ctgaagaatt tcggcctcga taccgtcgag cctgccgctg tcctcctcgg tgagacgctt
     2761 gaaaagaaaa tagagcaccc ggtcgaaagc gacctcctcc gccttttccg cataggatgc
     2821 gagctcccga agcatcctct cgctgtcgga aaaggccagc agccgcccct gggacaggta
     2881 cagtaccacc ggatggccgg agagggcgat ctttttgccg ttgagcaccg agagccggat
     2941 caggtcaaag ccgtcgtggc tctcaaattt tgccgcccgg cccgagagca gctcaggcag
     3001 aaagcgcgcg tcgatggcaa ggcggtcaaa gacctgctgc gcctgtgcga gcgggaacaa
     3061 cccgaactcc ggcccggcgg ccagcccctc ctcgccgtcg cgggcgggga caaagcgtcc
     3121 gtccttgatg tggtagatca ttccgcccca tccccctgtc cgccttgccc ctttgcaaag
     3181 ctctcccgct cgcggcgaag ctccgccagc agcgcggtta acgtccagcc ccggttgtgg
     3241 gcggtgacca ccgctttgag cgcgtccagg gcaaggccgc gcccgcgcaa atcgcccaac
     3301 agccgatcca gcgccgggtc ggaaaaggcg taaaaaacca gcgctttttc cgccggggcc
     3361 tgctgctgcg gcgcgccgga gggcggatag ccgggcagac ccagcagggc gcccagcgtt
     3421 ttgccgccgg cgtccggccc tgcaaagcgg acggccgcgc gcgcgccgcg gaacaattgt
     3481 tccagcgccg tgcgctccgg gccgtcctca tagctgtaaa gcaggatggt gccagtctgg
     3541 atacgtgctt tcaatccttt cacagtcctt tcaaacaggt ttggggccca tcagacccgc
     3601 gcgcacaggc gatgccgaca gcccagagcc gagctctcta ccggcctcaa aaagccagac
     3661 tcctcaaaaa attgtcaaag ctttctctgc gcaagcggcg gcctcccgct cattatttcc
     3721 cgatctctct gatcttatcg gcctcgacgc ttggcttccg gcttcacacg gcccaacgga
     3781 ctttcggcgg cgcccgccgt ttttatccac ggctttttat cgagggcggc tttttcattt
     3841 ttattttgaa agcgctgatc gccgcttttc cagcagacat accgtctcca catgccgtgt
     3901 gtgagggaac atgtccacgg gctggatgcg gcgcgcggcg tagccccggc cggtgagata
     3961 gtccagatcg cgcgcctggg tctccgggtt gcaggagata tagacggcct tttgcggccc
     4021 caggcgcacc agcgcctcca aaaaggcctc gctggcgccc tccctcggcg ggtcgaaaaa
     4081 aacgaggtcc gcgcgctctc cgtcctcagc catccgcgtc atcagctctc ccgcgtcccc
     4141 acagaggaat tcggcgttcc cgatcccatt cctgcgggcg ttgcgcacgg cgctgcggac
     4201 ggcttcggcg ttgagctcca cacccagcac ggcccttgcc cggccggccg cggccagggc
     4261 gatggtgccg atgccgcaat aggcgtcaat gacgcgctct tgcccggcga ggccggcgag
     4321 agcgagagcg gcggcgtaca gctgttccgt ctgcagcggg ttgacctgat aaaaggcgcg
     4381 cgccgcgacc gagaagcgca ggccgcagag ctcgtcctcg ataaagccct tgccgtacag
     4441 caccttttgc gcgctgccca gcacggcgct ggtgcgtcgc gggttgatgt tctgcaccac
     4501 cgtcgccacg tccgggcagg acgcgcgcag ggcgcgcaca aaatcacggc tgccgggaaa
     4561 gaagggggag ggggtgacga cggtcaccga gacctcgccc gtgtgcgccc cgcggcgcac
     4621 caccatatgg cgcagcacac cggtgccgcg gtcctcatcg aaagcgctga ggcggcagcg
     4681 gatcgcggca gagagggcgg cctccaccac ctcgttgatg cggctgtcct gcagtaggca
     4741 gtcccgtacg ggcacgaccc gatgggtgtg ggattcataa atcccgcaga ccggtttccc
     4801 ctttggaccg ggggcaaagg tggcgatggc cttgttgcgg tagtggaacg ggacctccat
     4861 gctcaaaatg gggtcgggcg cgcaaaagcg gccgagaaaa tagcccacca gctcctgctt
     4921 ttgccgcagc tgttcttcat atgggagcgc cagcagcgga catccgccgc agcgctgttt
     4981 tttggcgggg caatcgggcg tttttgtcat cgtcagtccc ccgccgagca cttggccgcg
     5041 tcgatggcca gcgcaatcat cagcaccagc agcgcgtttt cggggtcggg gaactccagc
     5101 acataggtgt cggtgagccg cagcagctcc ttggacagat gcgccacagg ctcgcccgcc
     5161 gcgtcggtga tctcgtagtc ccacgaaaag atgtcgccct cgacccgcca gccgttgcag
     5221 tccagctgaa aggcaggttt gaaaaaggtg aattcctttc ggatctcgcc caggcactgc
     5281 ccctgtgcat agatgcgaaa gcggggcaaa aaggtgagca cctcctcctt gaccgtgccc
     5341 aactgccgcc ccgaggcgtc caggatatcc aggcagtgcc caaaggagag cttgccctcc
     5401 accgtgtaga cggtattgcc gttttcgtca tagatgtcgt agctgtccag ccaggagaaa
     5461 aaacgctgtt taaaaagtag tttcataaag accctttctt gcctcgccgc cgggacggtc
     5521 cacagatcaa atgcagcgtc cggcgggaca aaaggcacgg cggatttttt attgctctga
     5581 gatgaagaat aatgttatta ttaagcgttg ctgtccatca accacctgga tcacttgctc
     5641 agtttttctg taaaatttga aacatcattc cgtcattgga cgcatcgtgg attcaactaa
     5701 tttgatttct ttattcgaga ggtcaaaata ttcatatagc tctcggtcag tccaagagcg
     5761 agtataatcc attttgggga caaaaatgaa attactctgt gaaatatgtg ccgaggaata
     5821 ggttgcccgc atcataaagc gcggcagttt gcacatcata tactttctaa aattttccgc
     5881 ttcggtttca ttgtcaaaaa atccaatgtt caaataagaa aatgaatcca cttgcccagg
     5941 atagacaata tgaggagtag acattgagcg atagcctttt tccggcttta ctcccgcctc
     6001 gcnnnnnnnn nnattctgtg gaactaaaat ggagatacgg atcttatatt tatcaatcaa
     6061 atgagcgttt ttagtgacaa catccctgga aatataactt gttttggaac tgttggcccc
     6121 atcactgtgg acgagcaaaa tatctccttc tatatacttt tcatgacctc gtacttttga
     6181 gggaatacca aatgtgtcga ttgcagaaac catttcatcg acaaagaaat cagcttttgt
     6241 cctgattttt tcaatgattg aaattgcttt gttagatcgt ataaacaaat ttccatactg
     6301 atttagagag cgcttgagtg tatcttggtt
//
LOCUS       DBMDYA010000063         6229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3201_length_6229_cov_1.326697, whole genome shotgun sequence.
ACCESSION   DBMDYA010000063 DBMDYA010000000
VERSION     DBMDYA010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_6229_cov_1.326697"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(357..1355)
                     /locus_tag="ACZPFK_02565"
     CDS             complement(357..1355)
                     /locus_tag="ACZPFK_02565"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIR1405227.1"
                     /translation="MKEFPKIDLHLHIDGSVQPKTALDLARGQGLPEGALSLEEVTRR
                     MVIQPGENGTDFKEFDLPIAVMQTASALRRVTAELVRRLAAEGVWYAELRFAPQFHLA
                     RGLSQREVIQAVLDGQADGQADCPDIETGILLCAMGTLAPAAENREQNLETVRLAKEF
                     IDAGSGVVGIDLAGYEDRLADYADVFALARELGVPATCHSEFEVPQCLPFGTPRIGHG
                     YQVAQRQELLQAVKARGVTLEMCPVSSIVSYDLPKDGRHPLKVLYDQGVRVTVNTDNL
                     VVLGTSLEREYECCRRMGFRDADLARMNLYSAEAAFLPPERKKRLVDRVRACLDEM"
     gene            1704..3752
                     /locus_tag="ACZPFK_02570"
     CDS             1704..3752
                     /locus_tag="ACZPFK_02570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1405228.1"
                     /translation="MQVKERGVLSLTPSRFKRALALVFSAGALLFLVLARDLFFYNGK
                     EFQLSYYLWSLALVPAIGLLIAFDLHLSKPAQGILSVFLAVISALPMFAMMEAFDVNT
                     IFKVLPYARLFNVLMIWLVCWVLYSVFGRFTVALRVADVLFYIMGLANSAVRIFRGTP
                     ISALDLYNIKTAAAVSGRYRYPFEFYFFTATLLMLFVWFLAGKATWRCERRAPRWIAR
                     GACLASVLAFGTVFSNTAYLNSIGIKDYLWNQNLAFYDNGLLLSLAYSTKYLNVEVPE
                     NYSAQAAQRVIDEADQPDPVAPAAQKPNIIAIMNESFADLAVVGEFETDVDYMPFIRS
                     MAGQPNTITGQLRVSTFGGGTCNTEFEFLTGNTLGFFPSGSVVYQQYIQRQTPSLATV
                     LKGQGYTADAVHPFYGYCWSRDKVYPLLGFDHFYDISDFSGARMIGSYAGNCVSDESD
                     FDKLIELFEAKVPGQPMFLFNVTMQNHGGYAQLINAPDELVHMTKDGYTSKYADNYLS
                     LIKATDTAFAQLIDYFSKVEEPTIILMFGDHEPNLPDGVYEALLGKSLDDLTLEETQK
                     RFTVPFVLWANYDIETQGGDAGLTSANYLSTLLMRTAGLETTPYMDYLAALSGDIPSI
                     NLNGYLGADGVEYGYDDRSPYTGLIEEYRTLQYNYLFDKKERLDRLFDWVPPAAADTK
                     "
     gene            3779..4549
                     /locus_tag="ACZPFK_02575"
     CDS             3779..4549
                     /locus_tag="ACZPFK_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1405229.1"
                     /translation="MDYDELLNTSTDVAFALLESGAEIYRVEEAIHRIFRAYGVEQGA
                     VFAIPTLLILSVQDDKGHPLTEIRRLGNHGSDLAKVDAYNNLCRQICQERPSFEAIRR
                     ELARIARGPRYSVLQQTAAFAMVAFGFTLLFGGGPADAVCSLLCGAAVRVVLYQFQRF
                     EANSFFTTIMASFAAAALAFAGVQSGLGQNLDKIIIGVLMNLVPGVALTNVMRDVIAG
                     DLIAGLTKLVEALLVATGIALGTGVALSLGRALLGVLA"
     gene            4546..5010
                     /locus_tag="ACZPFK_02580"
     CDS             4546..5010
                     /locus_tag="ACZPFK_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1405230.1"
                     /translation="MKELFLPCVYAFVACFAFCFVVNIRGRMLLSAPLGASLGRLVYI
                     AAAPVASELLCYFFATLAVSVYAEIMARFHRAPATGFLLVGLLPLVPGGGIYYTMEYC
                     ISGDTAMFLETGLHTFGIAGSLALGVLFVSSIVRFYSMVRRHLRRKEGAPPA"
     gene            5287..>6229
                     /locus_tag="ACZPFK_02585"
     CDS             5287..>6229
                     /locus_tag="ACZPFK_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405231.1"
                     /translation="MNGFLDRFFNPQKLVKKVPIAGPMPDASTMLTKAFKIAWPSLTE
                     SVLIGLVGMIDTMMVSTLGTYAIAAVGLTTQPKFIGLSFFLSLNIAVSALVARRRGEK
                     DADSAKQVLLQSLAITLLMTAVISTLCVVFADPILRLSGSAPDTHEDAVIYFRIIMGG
                     MVFNTISMVINAAQRGCGNTKIALRTNVTSNVVNIVFNYLLIGGNFGFPKLGVAGAAI
                     ATVIGTVAACVMSIISILHTNGFLRLSRPLKLRFDRRTLGGLANIGSSTLAEQIFLRI
                     GFFISAIIVARLGTEPFATHQICMNILSLAFCFGDGLS"
BASE COUNT         1099 a         2015 c         1770 g         1345 t
ORIGIN      
        1 cctgatgctc ttcgtccccg ccacctaccg caggcgctca tgaaaagcgg tctgcctgcc
       61 ccctgcggct tttcaaaaca ctaaaaaata gttgcaggct attttttcgg ccgtccggcc
      121 agtcccccgg ccggggccac taaaataagt tttttttatt gcggagcgat aaaaaatagc
      181 cgcaggctat tttttcggcc atccggccag tcctctggcc ggggccatcg agattagttg
      241 ttttttattg cgaagcaata aaaaataccg cgcggccccg gatcgtctgc cggggctgcg
      301 cggtattttc ttgtttttca ttcagacgcc ttttggcgcc ccgcagggct ggctttttac
      361 atctcatcca ggcaggcccg cacccggtcc acaaggcgtt tttttcgctc cggcggcaaa
      421 aaggctgcct ctgcgctgta gaggttcatc cgcgccagat cggcatcacg aaagcccatg
      481 cgccggcagc actcatactc ccgttccagg ctggtgccca gcaccaccag attatcggtg
      541 ttcaccgtca cccgcacacc ctggtcatag aggactttca ggggatggcg gccgtctttc
      601 ggcaggtcat aggagacgat gctgctcacc gggcacatct ccagcgtgac gccgcgcgcc
      661 tttaccgcct gcagcagctc ctgccgctgg gccacctgat acccgtggcc gatgcggggc
      721 gtgccgaaag gcaggcattg gggcacctcg aactcggaat ggcaggtggc gggcaccccg
      781 agctccctcg ccagcgcaaa cacatcggca taatccgcca gacggtcttc atatccggcc
      841 aggtcgatgc cgaccacccc gctccccgcg tcgatgaatt cttttgccag gcgcaccgtc
      901 tccaggttct gctcccggtt ctccgcggcg ggggccagcg tccccatggc gcagagcagg
      961 atgccggtct cgatatcagg acaatccgcc tgcccgtccg cctgtccgtc cagcaccgcc
     1021 tggatcacct cccgctggga gagcccccgg gcgagatgga actggggtgc aaagcgcagc
     1081 tcggcgtacc acactccctc cgccgccagg cgccggacga gttcggcggt caccctgcgc
     1141 agcgccgacg cggtctgcat cacggcgatg ggcaggtcga actctttgaa atccgtgccg
     1201 ttctccccgg gctggatcac cattcttctg gtgacctcct ccagcgagag cgcgccctcg
     1261 ggcaatccct gcccgcgcgc cagatccagc gcggtttttg gctggacgga gccgtcgatg
     1321 tgcagatgaa gatcaatttt cggaaactct ttcaaggggg acccctcctg tctgtcagtt
     1381 tgaaaacaag gcggcgcttt ttttgccgcg cccgcaaagc gcggcaaaaa gtcagctgtg
     1441 tcgacgttat acttttgttt tatcataaaa tccagaaaac ggcaagtgcc gctcatattc
     1501 cctaaaggac aaagcaaggg caaagcggct tctgccctga cttttcctgc cgggtgcgct
     1561 ttacttttca ggcctgatat gatatcctgt taactgtgta cataaaacag tacatttttt
     1621 gtggatatta cactaatgtg taatataatc ataggaaaac agggcgtctt ctcccctttt
     1681 tctttataaa ggagctgtcc ggattgcagg taaaggaacg aggggtcctc tccctcaccc
     1741 cgtctcgatt caaacgggcg ctcgcactgg tcttcagtgc cggcgctctg ctttttttag
     1801 ttctggctcg ggatctcttt ttttacaatg gaaaggaatt ccagctgtct tattatctct
     1861 ggtcgctggc gctggtgccg gccatcggcc tgttgatcgc tttcgacctc cacctttcca
     1921 agcccgcgca gggcattctg tccgtttttc tggctgtcat cagcgccctg cccatgttcg
     1981 ccatgatgga ggctttcgac gtcaacacca tcttcaaggt gctgccctat gcgcggctct
     2041 tcaacgtcct catgatctgg ctggtctgct gggtgctgta ctccgtgttc gggcgcttca
     2101 cggtggcgct gcgcgtcgcc gacgtcctgt tttacatcat ggggctggcc aacagcgcgg
     2161 tgcgcatctt ccgggggacg ccgatctcgg cgctggacct ctacaacatc aagaccgccg
     2221 ccgcggtgtc cggccgttat cgctacccgt ttgaatttta ctttttcacc gccacgctgc
     2281 tgatgctgtt tgtctggttc ctggccggca aagccacctg gcgctgcgag cgccgcgcgc
     2341 cccgctggat cgcccgcggc gcctgtctgg cctccgtgct ggctttcggg accgtgtttt
     2401 ccaacacggc gtacctcaac agcatcggca tcaaggacta tctctggaac cagaacctgg
     2461 ccttttacga caacggcctg ctgctcagcc tcgcctacag cacaaagtac ctgaacgtgg
     2521 aggtgcccga aaattacagc gcccaggccg cgcagcgggt gatcgacgag gcggaccagc
     2581 cggacccggt ggcgcctgcg gcgcaaaagc ccaacatcat cgccatcatg aacgagagct
     2641 ttgccgatct cgcggtggtg ggagagtttg agaccgatgt ggactatatg ccctttatcc
     2701 gcagcatggc cggccagccc aacaccatca ccggccagct gcgggtcagc acgttcggcg
     2761 gcggcacctg caacacggag ttcgagttcc tgaccggcaa cacgctgggc tttttcccct
     2821 ccggcagcgt ggtctatcag cagtatatcc agcggcagac gccgtccctt gccaccgtgc
     2881 tcaagggcca gggatacacg gccgacgcgg tgcacccgtt ctatggctac tgctggagcc
     2941 gcgacaaggt ttacccgctg ctgggcttcg accattttta tgacatctct gatttttccg
     3001 gcgcgcggat gatcggcagc tatgcgggca actgcgtgag cgacgagtcg gatttcgaca
     3061 agctgatcga gctgtttgag gccaaggtcc ccggccagcc gatgttcctc ttcaacgtga
     3121 ccatgcaaaa ccacggcggc tacgcccagc tcatcaacgc cccggacgag ctggtccaca
     3181 tgaccaagga cggctacacc agcaaatacg cggacaacta cctctcgctc atcaaggcca
     3241 ccgacacggc ctttgcacag ctgatcgatt acttcagcaa ggtggaagag ccgaccatca
     3301 tcctgatgtt cggcgaccac gagcccaacc tgccggacgg cgtctatgag gcgctgctgg
     3361 gcaaaagcct ggatgacctg accctggagg agacccaaaa gcgcttcacc gtgccgtttg
     3421 tcctgtgggc caattatgac atcgagaccc agggtggcga cgccggcctg accagcgcca
     3481 attatctctc caccctgctc atgcgcaccg cgggccttga gaccaccccc tatatggatt
     3541 acctcgcagc cctctccggc gacatcccct ccatcaacct gaacggctat ctgggcgcgg
     3601 acggcgtgga gtacggctat gacgaccgct cgccctatac cggcctgatc gaggagtacc
     3661 gcactctgca atacaactat ctgtttgaca agaaagaacg tctggaccgg ctttttgact
     3721 gggtaccgcc ggctgccgcc gacacaaagt gacctgacga aagagaggcg cattgccctt
     3781 ggactacgac gagctgttga acaccagcac cgacgtcgcc tttgccctgt tggagagcgg
     3841 cgcagagatc taccgggtgg aggaggcgat ccaccgcatc ttccgcgcct atggggtcga
     3901 gcagggcgca gtcttcgcca tccccaccct gctcatcctg tcggtgcagg acgacaaagg
     3961 gcacccgctg accgagatcc ggcggctggg caaccacggc agcgaccttg ccaaggtgga
     4021 cgcctacaac aacctctgcc ggcagatctg ccaggagcgc ccctcttttg aagcgatccg
     4081 ccgcgagctg gcgcgcatcg cgcgcgggcc ccgttattcc gtgctgcagc agaccgcggc
     4141 gtttgcgatg gtggcgttcg gcttcaccct gcttttcggc ggtggccccg ccgacgccgt
     4201 ctgctcgctg ctctgcggcg cggcggtgcg ggtggtatta taccagttcc agcgctttga
     4261 ggcgaattcc ttttttacca ccatcatggc cagctttgcc gcggcggcgc tggcctttgc
     4321 gggggtccag tccggcctgg ggcaaaatct cgacaagatc atcatcggcg tgctgatgaa
     4381 cctggtgccc ggggtggcgc tgaccaatgt gatgcgggac gtgatcgccg gcgacctgat
     4441 cgccgggctg accaagctgg tggaggcgct gctggtggcc acgggcatcg ccctgggcac
     4501 cggcgtggcg ctcagcctgg gccgcgcgct gctgggggtg ctggcatgaa agagttgttt
     4561 ttgccctgcg tctatgcctt tgtcgcctgc ttcgcctttt gttttgtggt caacatccgc
     4621 ggcaggatgc tgctgtccgc gcccctcggg gccagcctgg gcaggctggt ctatatcgcc
     4681 gccgccccgg tggcctccga gctgctctgt tactttttcg ccaccctggc ggtctcggtt
     4741 tacgccgaga tcatggcgcg gttccaccgg gccccggcca ccggctttct gctggtgggg
     4801 ctgctgcccc tggtgcctgg gggcggcatc tattacacga tggaatactg catcagcgga
     4861 gacaccgcca tgttccttga gaccgggctg cacacgttcg gcatcgcagg ctccctggcc
     4921 ctgggggtgc tgttcgtctc cagcattgtc cgtttttaca gcatggtgcg ccgccacctg
     4981 cgccgcaagg agggggcccc gcccgcctga cgggcggcgt gacggcaggg gcgccgccga
     5041 aagcgctctc cgacaagggg cgcggccgag gcgcttttgc cctgtgggtc gaaagccttt
     5101 gcgcttctct tcgcgtcccg cgcttttccc cgcccctgca aatcattcta tcttttcgcc
     5161 gggattccag ctggaatccc ggctttttta cacggatttt acaaaaagcc tcaaagactt
     5221 tgaaaagtat tacgttttag tgtagaatga tggtgtatga tatcagaaag ccaggtgtac
     5281 agtcgaatga atggattttt agaccgattt ttcaacccgc aaaaacttgt gaaaaaagtc
     5341 cccatcgcag gccccatgcc cgacgcttcc accatgctga ccaaagcgtt caagatcgcc
     5401 tggccctcgc tgaccgaatc ggtgttgatc ggccttgtgg gcatgatcga caccatgatg
     5461 gtgagcacgc tgggcaccta cgccatcgcg gcggtcggcc tcaccacgca gcccaaattc
     5521 atcgggctct ctttttttct gtccctcaac atcgccgttt ccgcgctggt ggcccggcgg
     5581 cggggcgaaa aggacgccga cagcgctaaa caggtgctgc tgcagtcgct ggcgatcacg
     5641 ctgctgatga ccgccgtcat cagcaccctc tgcgtcgtct tcgccgaccc catcctgcgg
     5701 ctgtccggct ctgcgcccga cacccatgag gacgcggtca tctatttccg tatcatcatg
     5761 ggcggtatgg tcttcaacac gatctccatg gtcatcaacg cggcccagcg cggctgcggc
     5821 aacaccaaaa tcgccctgcg caccaatgtg accagcaacg tggtgaacat cgttttcaac
     5881 tacctgctca tcggcggcaa tttcggcttt cccaagctgg gggtggcggg cgccgccatc
     5941 gccactgtga tcggcacggt ggcggcctgc gtgatgagca ttatcagcat cctgcacacc
     6001 aacggctttt tgcggctcag ccgcccgctc aagctgcgct ttgacagacg cacgctgggc
     6061 ggcctggcca acatcggctc ttccaccctg gcggagcaga tctttctgcg catcggcttt
     6121 ttcatctcgg ccatcatcgt ggcgcgcctg ggcaccgagc cctttgccac ccaccagatc
     6181 tgtatgaaca tcctcagcct ggctttctgc tttggcgacg gcctctcgg
//
LOCUS       DBMDYA010000064         6216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3209_length_6216_cov_1.599019, whole genome shotgun sequence.
ACCESSION   DBMDYA010000064 DBMDYA010000000
VERSION     DBMDYA010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3209_length_6216_cov_1.599019"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="ACZPFK_02590"
     CDS             <1..219
                     /locus_tag="ACZPFK_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1405232.1"
                     /translation="DADKAVTAVLDSITAALVDGDKVALVGFGTFETRKRPARKGRNP
                     RTKEEITIPATVAPAFKAGKALKDAVAK"
     gene            769..2151
                     /gene="cysS"
                     /locus_tag="ACZPFK_02595"
     CDS             769..2151
                     /gene="cysS"
                     /locus_tag="ACZPFK_02595"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1405233.1"
                     /translation="MKIFNTMSRQKEEFVPMKEGEYRIYVCGPTVYNYIHVGNARPVC
                     VFDTLRRYLEYRGNRVLYVSNITDIDDKIIKKANEEGTTYDVIARRYEEEYFKDCRGL
                     NVKPATVHPRATEHIAEIQSIIKDLIEKGYAYPAGDGDVYFRARKDAHYGKLSHQPLE
                     DLESGARIAVGEIKEDPLDFAVWKGSKPGEPAWDSPWGPGRPGWHIECSAMARKHLGD
                     TIDLHCGGVDLVFPHHENEIAQSECANGCTFARYWMHNGFLNIDNHKMSKSLNNFFTV
                     RDVAEQVGYEPIRFFLISGHYRTPINYTKEVIESCKASLERLYTCRDNLDFAIAHASG
                     GDTALAERADAAQNKFIAAMDDDLNTADAIASVFELARDINTLSAASSKDTLEKAARV
                     FDELTGVLGLLYNRARDEAPARVKELAAERAEARKAKDWPRADAIRDELASLGYAVED
                     TPSGPKIVKK"
     gene            2188..2436
                     /locus_tag="ACZPFK_02600"
     CDS             2188..2436
                     /locus_tag="ACZPFK_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1405234.1"
                     /translation="MRIDKYLKVSRLIKRRTVANEVADAGRITVNGRPVKASYAVSEG
                     DVIEVQFGQKPLRVKVTSVEPPSGKDVAREMYVVLEEE"
     gene            2739..3071
                     /locus_tag="ACZPFK_02605"
     CDS             2739..3071
                     /locus_tag="ACZPFK_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1405235.1"
                     /translation="MMQPQAKNVFRADGGAAAPASAAPQTVRHNLIVENRSNLTATGI
                     RQVVSYDEYSATVLTDLGTLVIGGKNLKVSELSCQSGELRVSGEIEYIQYEDKREKKG
                     GFLGRLTR"
     gene            3071..3541
                     /gene="yabQ"
                     /locus_tag="ACZPFK_02610"
     CDS             3071..3541
                     /gene="yabQ"
                     /locus_tag="ACZPFK_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1405236.1"
                     /translation="MVTQVPLPLQGMDAFSALGLGLLLAALYDAVRFFLGGAKPVVFL
                     CDLLFFALSAAAAISFGVSYSYTGFLRWYIVAGMAAGYAAYFFAVAPFTAALRRGVLF
                     VLLLPWRLLRRYLFVPLGKKIGEQHQKHKKKRREKKKRKKEPLQPAGKVLYNSN"
     gene            3594..3896
                     /locus_tag="ACZPFK_02615"
     CDS             3594..3896
                     /locus_tag="ACZPFK_02615"
                     /inference="COORDINATES: protein motif:HMM:NF016839.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1405237.1"
                     /translation="MRKKDSGRGLNLSGFVLRVSAAGLIVYLAVTLIISQVDIMVKRQ
                     ELGSLNTDLARQMEQNTELERLISSGGEDEYMERIARDRLGYAAPGERIFIDMSGK"
     gene            3934..4350
                     /locus_tag="ACZPFK_02620"
     CDS             3934..4350
                     /locus_tag="ACZPFK_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1405238.1"
                     /translation="MAVEVGSIVEGKVTGIKKFGAFVQLPDGKTGMVHISEVSNEYIQ
                     ELSTVLSEGQQVKVKVMSVSPEGKIALSIKRTMPRPKPTRADTGKVWQPRQQAPQGDL
                     SFEDMMAKFKSQSEEKISDLKRVTEARRGGGYSRRR"
     gene            4641..5159
                     /gene="hpf"
                     /locus_tag="ACZPFK_02625"
     CDS             4641..5159
                     /gene="hpf"
                     /locus_tag="ACZPFK_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR1405239.1"
                     /translation="MKISTTGRKVSLKPAFVERADKRLSKLDKFFSDEAAAQVTVTVE
                     KDWQTVEITVRDKGFVARAEKSAENMEDALDAAVEVLTKQIVKNRKRLETKLYKAAFD
                     DYAGAEQEEESFAVVREKHFYVKPASVDEAILQMNLIGHSFFLFRNVDSDEINVVYRR
                     KNGTYGLLIPKE"
     gene            5365..>6216
                     /locus_tag="ACZPFK_02630"
     CDS             5365..>6216
                     /locus_tag="ACZPFK_02630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1405240.1"
                     /translation="MTYEFVAPCYFGTERSAAFDFKKIGASDVTVTDGRVSFKGDERV
                     LAAANLQSRCAERILLLLKRCRTATFDELFDAVSSVPWEALLPADAEFPVKGSSLSST
                     LSSVPACQSIVKKAIVERLKRGHRVQVLPEDGQLYKVRFSIRKDVMELFLDTSGDGLH
                     KRGYRARATEAPIKETLASAIVDLARVRGDSLVQDPFCGSGTIVIEAAQKALRMAPGL
                     RRRFLAEHYAFVPPQVWRDAREQALAEVDKTAAFEGVGFDIDPHAVELANRNAKLAGV
                     GERCRFFV"
BASE COUNT         1266 a         1870 c         2021 g         1059 t
ORIGIN      
        1 gacgccgaca aggccgtcac cgctgttctg gattccatca ccgccgcgct ggttgacggc
       61 gacaaggttg ccctcgttgg tttcggcact tttgagaccc gcaagcgccc ggcgcgcaag
      121 ggccgcaatc cccgcaccaa agaggagatc accatccccg cgaccgtggc tcccgctttc
      181 aaggccggca aagccctgaa agacgccgtc gcgaaataag acctcgcacc aaaatcagag
      241 gcgccccttt gagggcgcct cttttttgcg cccgggcggc cctggggccg gggcgccgag
      301 agggggccgc cgcctctcgg ctttttcggt gcgcgcgcag actgcggctg ctccggcggg
      361 cgggtgaaaa cagcctcctg tgcggtttga cgacgcgcat aaaacctgct ataatgagcg
      421 acaataaggc aaatgcgatg gcgcgctttt tgcgcgccgg caccgggaga agtaccggca
      481 gggcagacgc tgcagagagg gcgcgcagcg cggagggagg cctccgcacc gctgcgagcg
      541 cgccccgtcc ccgcccgccg cgaaatgcac ccggcagcac gggcagtgaa cgggcctccg
      601 gccccgctag ccgcctgcgg acgcccccgt tatcaagggc taagagtaat aagccgaagt
      661 ggaaccgcgg cattccgccg ccttcgtccg gcccccatgg ggcgggcggg ggcttttttt
      721 gtgcccggtc ttcggcagct ttgaaaggaa atgagagaga tggcacacat gaagatcttc
      781 aacaccatga gcaggcaaaa ggaggagttc gtccccatga aagagggcga ataccgcatc
      841 tatgtctgcg gccccaccgt ctacaactat atccatgtgg ggaacgcccg ccccgtctgc
      901 gttttcgaca cgctgcgccg ctatctggag taccggggca accgggtgct ctatgtctcc
      961 aacatcaccg acatcgacga caagatcatc aaaaaagcca acgaggaggg caccacctat
     1021 gatgtgatcg cccgccgcta tgaggaagaa tattttaagg attgccgcgg cctgaacgtc
     1081 aagcccgcca ctgtccaccc ccgggccacc gagcacatcg cggagatcca gagcattatc
     1141 aaggacctga tcgaaaaggg ctacgcttac cccgcggggg acggggacgt ctatttccgc
     1201 gcccgcaagg acgcccatta cggcaagctg tcccatcagc cgctggagga tctggagagc
     1261 ggcgcgcgca tcgcggtggg cgagatcaag gaggacccgc tggactttgc ggtctggaaa
     1321 ggctccaagc ctggcgagcc cgcctgggat tccccctggg gccccggccg tcccggctgg
     1381 cacatcgagt gctcggccat ggcccgcaaa catctgggcg acaccatcga cctgcactgc
     1441 ggcggcgtgg acctggtttt tccgcatcac gagaacgaga tcgcccagtc cgagtgcgcc
     1501 aacggctgca cctttgcccg gtattggatg cacaacggct ttttgaacat cgacaaccac
     1561 aagatgagca aaagcctgaa caactttttc accgtgcggg acgtggcgga gcaggtgggg
     1621 tatgagccga tccgcttttt cctcatcagc ggacattacc gcacccccat caactacacc
     1681 aaggaggtca tcgagagctg caaggccagc ctggagcggc tgtacacctg ccgtgacaat
     1741 ctggattttg ccattgccca cgcctccggc ggcgataccg ccctggcgga gcgggccgac
     1801 gccgcgcaga acaagttcat cgccgccatg gacgacgacc tgaacaccgc cgacgccatc
     1861 gcctcggtgt ttgagctggc gcgggatatc aacaccctgt ccgccgcctc ctccaaggac
     1921 acgctggaaa aggcggcccg ggtctttgac gagctcaccg gggtgctggg cctgctctat
     1981 aaccgggcca gggacgaggc gcccgcgcgg gtgaaagagc tggcggccga gcgcgccgag
     2041 gcgcgcaagg cgaaagactg gccgcgcgcg gatgcgatcc gggatgagtt ggcctcgctg
     2101 ggctacgcgg tggaggatac ccccagcggc cccaagatcg taaaaaaatg acatgcgccg
     2161 ggcgcaccgg cgcagaggag agcagctatg cgcattgaca agtatttgaa agtctcccgc
     2221 ctgatcaagc gccgcactgt ggcgaacgag gtggcggacg cgggccgcat caccgtgaac
     2281 ggcaggccgg tcaaggcgag ctatgccgtg agcgagggcg atgtcatcga ggtgcagttc
     2341 ggccaaaagc cgctccgggt caaggtgacg tcggtggagc cgccctccgg caaggatgtg
     2401 gcgcgggaga tgtacgtggt tctggaagag gaataaaaac cgttgggaaa ggcgcggcgc
     2461 gaatgccgcg cctttcccgt tttccagggc tgggcgctgc gtcgtgcggg tggggagcgc
     2521 aaaagccgcg gcggtcggat tggggcaatg gcgcggggtt gagcggggag ttgacggccg
     2581 ccgtcggggg acgcgtatac gcgcgcatat ggccgggctg gtccgcatag agatagggta
     2641 caaggctctg cgcggcggcg gcccttttga ggagggcccc catcccgcgc ggcgatcagg
     2701 ggcggcgcgc cgcccacagg aagggaagag ggatctctat gatgcagcca caagccaaaa
     2761 acgtattccg cgccgacggg ggggcagccg cgcccgcctc ggcggccccg cagacggtgc
     2821 ggcacaatct catcgttgaa aaccggtcaa acctcaccgc cacgggcatc cgtcaggtgg
     2881 tctcttatga cgagtactcg gccaccgtgc tcaccgatct cggtaccctc gttatcgggg
     2941 gcaaaaacct caaggtcagc gagctgtcct gccagagcgg cgagctgcgg gtcagcgggg
     3001 agatcgagta catccagtat gaggataaaa gagaaaagaa aggcggcttt ttgggccgcc
     3061 tgacccgctg atggtcactc aggtccccct gcctctgcag gggatggacg ccttttccgc
     3121 gctggggctg gggctgctgc tggcggccct gtacgacgcc gtgcgctttt ttctgggcgg
     3181 ggcaaagccg gtggtattcc tctgtgattt gctctttttc gccctctcgg ccgcggcggc
     3241 gatcagcttc ggcgtgtcct acagctatac cggctttctg cgctggtaca tcgtggcggg
     3301 tatggcggcc ggctatgcag cctatttttt tgccgtcgcg cctttcaccg cggcgctgcg
     3361 gcggggggta ttgtttgtgc tgctgctgcc ctggcggctc ttgaggcggt atctgtttgt
     3421 cccgctgggc aaaaagatcg gcgaacagca ccaaaaacac aaaaaaaagc gccgcgaaaa
     3481 aaagaaaaga aagaaggaac ccttgcaacc ggcagggaaa gtgttgtata attcaaatta
     3541 acagtaagca aagggcttat tgtgcccatt tttaaacgga ggggggctgc gggatgcgca
     3601 aaaaggacag cggcaggggg ctgaacctgt cgggcttcgt gctgcgggtc agcgcggcgg
     3661 gcctcatcgt ttatctcgcc gtcaccctga tcatcagcca ggtggacatc atggtcaagc
     3721 gccaggagct gggttcactg aacaccgact tggcccgcca gatggagcag aacaccgagc
     3781 tggaacggct gatctcttcc ggtggggagg acgagtatat ggagcggatc gcgcgggacc
     3841 ggctgggcta tgcggccccc ggtgaacgca tctttatcga catgtccggc aaatgagccg
     3901 gagccacaca aagagaagga gaaattcggg tacatggcag ttgaggtagg gagcatcgta
     3961 gagggcaagg tcacagggat caaaaaattc ggcgcgttcg tccagttgcc ggacggcaag
     4021 accggcatgg tacacatctc agaggtttcc aacgagtata tccaggagct gagcaccgtg
     4081 ctgagcgagg gccagcaggt caaggtcaag gtgatgtcgg tctcgccgga gggcaagatc
     4141 gcgctttcca tcaagcgcac catgccccgt cccaagccga cccgcgccga cacaggcaag
     4201 gtttggcagc cgcgccagca ggcgccgcag ggcgatctga gctttgagga tatgatggca
     4261 aaattcaaga gccagagcga agagaaaatc tccgacctca agcgggtgac cgaggcccgc
     4321 cgcggcggcg gttattcccg cagaagataa gcagataaaa aaggccgggg ctgccccggc
     4381 ttcttttatg cctccctgcg aaaaagtagg gcggcggcac aaaaaagctc tgcgccgccg
     4441 cctggggaat gcttttacag tttaacccgg gcggcgcaaa aaaacggtcg aagcgtgccg
     4501 ctccgtccag gggcgtcaca gcttcacaaa tatttgtcaa attgaccgta aaatcttctt
     4561 ttatgcccgg gcgttttgtg atataatcat aacaggaacc cggcaacggg gccagtagct
     4621 ctcagctagg aggtattgtt atgaagatca gcaccacggg cagaaaagtc agtttgaaac
     4681 cggcgttcgt cgagcgcgct gacaagcgcc tttccaagct ggacaagttc ttctccgacg
     4741 aggccgccgc acaggtgact gtgacggtgg aaaaagactg gcagaccgtc gagatcacgg
     4801 tgcgtgacaa gggctttgtg gcccgcgctg aaaaaagcgc tgagaatatg gaggacgccc
     4861 tggacgcggc ggtggaggtc ctcaccaaac agatcgtcaa aaaccgcaag cggctggaga
     4921 ccaagcttta caaggccgct ttcgacgatt acgccggggc cgagcaggag gaggaatcct
     4981 ttgccgtcgt gcgcgaaaag catttttacg tcaagcctgc cagtgtggat gaggccatcc
     5041 tgcagatgaa cctgatcggg cattcgttct tcttgttccg caatgtggac agcgacgaga
     5101 tcaacgtggt ctaccgccgc aaaaacggca cctacggcct gctaatcccc aaggaataaa
     5161 atcacctgcc gcggcatggg ttgcggggcc ccagtggccc tgcggcgccg ggccgcgaaa
     5221 agagaaaaga ccgcaggaga ggggcggagc gttccgcctc tctctttttg agcaaaaacg
     5281 ggcgtttgac gggacgaaag cgcgggtgca aaggcgccgg cggtcccgca tcggcagacg
     5341 cccctcacag aaaggaagct ttccatgacc tatgagtttg tcgccccctg ttatttcggc
     5401 accgagcgca gcgccgcctt tgactttaaa aagatcggcg ccagcgacgt caccgtgacc
     5461 gacggccgcg tctcctttaa aggggacgag cgggtgctgg ccgcggccaa tctccagagc
     5521 cgctgtgccg agcgcatcct gctgctgctc aagcgctgcc gcaccgccac ctttgacgag
     5581 ctgttcgacg ctgtgtcctc cgtgccctgg gaggcgctgc tgccggcgga cgcggagttc
     5641 ccggtcaagg gctcttcgct ttcctccacg ctctccagcg tcccggcctg ccagagcatc
     5701 gtcaaaaagg ccatcgtcga gcgcctcaag cggggccatc gcgtgcaggt actgcccgag
     5761 gacggccagc tgtacaaggt gcgcttttcg atccgcaagg acgtgatgga gctgtttctc
     5821 gacaccagcg gggacgggct gcacaagcgg ggctaccggg cgcgcgccac cgaggcgccc
     5881 atcaaggaga ccctggcctc ggccatcgtc gatctggccc gggtgcgggg cgacagcttg
     5941 gtgcaggacc ctttctgcgg cagcggcacc atcgtcatcg aggcggccca aaaggccctg
     6001 cgcatggccc ccggcctgcg ccgccgcttt ctggccgagc attacgcctt tgtgccgccg
     6061 caggtctggc gcgacgcgcg ggagcaggcc ctcgccgagg tggacaagac cgccgcgttc
     6121 gagggggtgg ggtttgatat cgacccccat gccgtggagc tggccaaccg caacgccaag
     6181 ctggcgggcg tgggggagcg ctgccgcttt tttgtg
//
LOCUS       DBMDYA010000065         6199 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3221_length_6199_cov_1.235651, whole genome shotgun sequence.
ACCESSION   DBMDYA010000065 DBMDYA010000000
VERSION     DBMDYA010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_6199_cov_1.235651"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            35..292
                     /locus_tag="ACZPFK_02635"
                     /pseudo
     CDS             35..292
                     /locus_tag="ACZPFK_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012497.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
     gene            complement(398..1123)
                     /locus_tag="ACZPFK_02640"
                     /pseudo
     CDS             complement(398..1123)
                     /locus_tag="ACZPFK_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(1347..3251)
                     /locus_tag="ACZPFK_02645"
     CDS             complement(1347..3251)
                     /locus_tag="ACZPFK_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021615.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HIR1405241.1"
                     /translation="MHSPSRPLPQTLRLVLQTYLGILPGLLLFEILYKALAALALRPA
                     LSWMTNTLLGLGGRGLVFNENILSIFTNLLGLLAAALLAALATALSYYEFSALFLSAH
                     CRACGRPVSFRLALELGFTAMHGLKSPWSLFFAVYVLGLLPLADLGLSPSLLPALRIP
                     SFITGELAKTSLGGVLVALFYAAVLAVFLLLLFALPAMILGREPFGPAARRSLTAARL
                     LGWRGWALCGGFAALWWVLFRSPGLVPTHFVGITGAGFHEFTANLLNGRILSSLPAFA
                     LSGLLRIALTLLLVSLLTVLYAQLDGPAQLDETRLPAISARVDRTQLAAQRLWQRARR
                     GASSLWALVRKRPFYQKHKKLVWAALVFVALWIITDIFTVPPGLHAPIAVGHRGSAQG
                     VENTVEAVQGAIDAGADYAEIDVLLSKDNVPMVIHDADLSRLAGTRGGVYDFTAEELQ
                     KLTLSQNGFTGRISTLEEMLGFCDGKIRLVVELKTHGRESGDVAALVAETVERAGAGE
                     RCIFMSIDYAMAQRLRELRPQWVVGYCVYGNVGGLSTGALLELDVDFLTIEESMVNPR
                     FVGQCLQAGLPVYVWTVDDPGKMETYLKTGVNGIVSDDPALAKEAVSGYGGEPMEEFY
                     RWQKGDTPDF"
     gene            complement(3632..5740)
                     /gene="rnr"
                     /locus_tag="ACZPFK_02650"
     CDS             complement(3632..5740)
                     /gene="rnr"
                     /locus_tag="ACZPFK_02650"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR1405242.1"
                     /translation="MKELEKRPRRLKELKAKFDEDKKVQAVLGFLVKDGLVVEKDGVY
                     FRKARSAQPATTGEAIKCTVVKVAKNFGFAAPVVEEDEQGNLPAEKPADFFVPGRFLL
                     GAMPGDVVLVKRLVGGRSEDEGQVVAIVEENNKLVGVVDRQEGRLVLLPDRCPALPLP
                     IKKSADGGAEVGEKVAAEILERGESHRDHRVGISQCFGSSDSAKSCTKAILYANDIVK
                     SFPGKVKAEAKRLEDAEVKQGDLKGRKDFRDRVIFTIDSADTKDIDDAISLARTETGY
                     ELGVHIADVSHYVVPGTALDDEAYHRATSVYTPGAVVPMLPRQLSNGICSLNPGVDRL
                     CFSCVMELDVTGKVTDFSFYKAVMRSRVKGVYSEINSIYAGLAGQDLQQKYAECLPTL
                     ELMRELYDKLAKRRAARGCMEIETGEAKIILDADGKAVDVKKVERGVAEQMIEEFMLL
                     ANTSAAKLGKKLKVPFLYRVHELPDPDRVERLHDALTAMGLDARFAGETPTQLELSKL
                     LNDARGTSLERAVHINVLRSMAKAKYLSEPKGHFGLALPDYTHFTSPIRRYPDLIVHR
                     ILSAALAGATEKELTGKYGEFVVEAARHTSEREVLAMVSERSADDCYKAEYMADKIGG
                     EFEGTISSVTNFGIYVELENTVEGLVHVSRLSDGEMNLSNSMALVDSLTGKSYRIGDP
                     VRVRLIGVSISAGNVDFELA"
     gene            complement(5967..>6199)
                     /gene="secG"
                     /locus_tag="ACZPFK_02655"
     CDS             complement(5967..>6199)
                     /gene="secG"
                     /locus_tag="ACZPFK_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1405243.1"
                     /translation="VGGVLLILASLVIIFMVLVQQPSGNGLSGAIGGGEAMTSEARTR
                     TTDAVLAKYTKIAAIVFFVVTLAVSLFSVFAK"
BASE COUNT         1044 a         2081 c         1962 g         1112 t
ORIGIN      
        1 ggtgctctgc gccgtggcgg cctgcggggg cgcggtgacg ctgcggcggg ccccgtttgc
       61 gccgcttcag ccgctggcgg acctgctgcg gcgggcgggc tgcgcggtgc agcccctgcc
      121 ggacgacggg gtgcagatcg aggccgtgga tcggctgagc ggcctgggag agctggagac
      181 cggcgtctac cctgcctttg ccaccgacgc cgggccgctg ctggcggccg cgatgctgac
      241 ggcggagggc gacaccgccc tgcgggagac cattttcacc aaccgcttcg cctgtgccgc
      301 cgagtttgcc cgcatgggcg cggacgcgcg ggtgagcggc gacggcgggc atgccctcgg
      361 ggatggccgc caccgacagc gaaatgccgg tgaggatcat gttcatcagg ctctcgccct
      421 tgaaatagcc gatggcgctg accgccaggc agaccacgat gcaggccacg gccacaaatt
      481 tgcccagctc gtcgagccgc tgctgcaggg gggtcttttc ctccttgtgc tcctccagca
      541 tgccggcgat gcggcccatc tcggtgtcca tgccggtggc ggtgacccgc gcctcgcagt
      601 gcccggccgc caccgtggcg cccatataca gcttgccgcc gtttttcttc gccaccggca
      661 cactctcgcc ggtgatgatg gactcgtcgc tggagagggc cacgcaggcc gccgcctcgg
      721 cgtcggcggg gatgcggtcg ccagccgtca gggacagcag gtcgccgggc accagctcct
      781 cggcgggcag cgtgaccagc tggccgtccc gccaggcctt ggccaccggg gcggagagcg
      841 cgttcagcgc ctccagcgtc ttttcggtgc ggtattcctg cacaaagccc atgacggcgt
      901 tgagcagcac gatggcgatg atggtgatgg cctcggtggt ctcgcccagc agcgccgaca
      961 gcaccgtggc aatggcgagg atgatgatca tgaggtcctt gaactgcgcc agaaacatgc
     1021 cgaccgccga ggtgcctttc tttttggaca aggcgtttgg cccgtgctgt tccagcctct
     1081 gttttgcctc ctgtgaggag agtcccctgc cgcctgtctg catattgcaa aatccccttt
     1141 ttgtccgttc tctcttccct cccggctgaa cgctggcagc gcgggcttct cctcaaaatc
     1201 tatgaggcgg caagggagga ttatgacggc ggggccgggg cggcgcaaaa aaggccttgg
     1261 gcgggcccgt tcagggcccg cccaaggcgt cggttccgcc cgggcggcaa ggcgccgccc
     1321 gggcaatttt ttcatctgcc gccgggtcaa aaatcagggg tatccccttt ttgccagcga
     1381 taaaattcct ccatcggctc tcccccatac ccgctcaccg cctccttggc aagcgctggg
     1441 tcgtcggaaa cgatgccgtt caccccggtc ttgagataag tctccatctt gccgggatcg
     1501 tccaccgtcc acacatagac gggcaggccc gcctgcaggc actggccgac aaagcggggg
     1561 ttgaccatgc tctcctcgat ggtgagaaaa tccacatcca gctccagcag cgccccggtg
     1621 ctcaggcctc ccacgttgcc gtagacgcaa tagcccacca cccactgggg ccgcagctcc
     1681 cgcagacgct gcgccatggc gtagtcgatg gacatgaaga tacagcgttc cccggccccg
     1741 gcgcgctcca ccgtctccgc caccagagcg gccacgtctc cgctctcgcg gccatgggtc
     1801 ttcagctcca ccaccagacg gattttccca tcgcaaaatc ccagcatctc ctccagcgtg
     1861 gagatgcgcc cggtaaagcc gttttgggag agggtgagct tttgcagctc ctcggcggtg
     1921 aagtcgtaca cgcccccgcg cgtgccggcc agccgtgaga gatcggcgtc gtggatgacc
     1981 atgggcacgt tgtcctttga gagcagcacg tcgatctccg cgtaatccgc ccccgcgtcg
     2041 atggcgccct gcacggcctc aacggtgttt tccaccccct gggcgctgcc gcggtgcccc
     2101 acggcgatgg gcgcgtgcag ccccggcggc acggtgaaga tatcggtgat tatccagagc
     2161 gccacgaaca ccagcgccgc ccacaccagt tttttgtgtt tttgatagaa cggtcgcttt
     2221 cgcaccagcg cccacaggga cgacgcgccg cggcgcgccc tctgccagag cctctgcgcc
     2281 gccagctgcg tccggtccac ccgggctgag atcgccggca gccgggtttc atccagctgc
     2341 gcggggccgt ccagttgggc gtaaagcacg gtcagcaggg agaccaaaag cagggtgagg
     2401 gcaatgcgca gcaggccgga cagggcaaac gcgggcagcg aggagaggat gcggccgttg
     2461 agcagattgg ccgtgaactc gtgaaacccc gccccggtga tgcccacaaa atgggtgggc
     2521 accaggccgg gcgaccgaaa caagacccac cacagtgccg caaaaccgcc gcagagagcc
     2581 cagccccgcc agcccaacag ccgcgcggcg gtcaggctgc gccgcgccgc cggcccgaaa
     2641 ggctcgcgcc ccaggatcat ggccggcagg gcgaagagca gcagcaggaa caccgccagc
     2701 accgccgcat agaacagcgc caccagcacg ccgcccagcg acgtcttggc cagctcgccg
     2761 gtgatgaagc tcgggatgcg cagcgccggc agcagcgagg gggaaagccc caggtccgcc
     2821 agcggcagca agcccagcac gtacacggca aagaacaggc tccaggggct tttcagccca
     2881 tgcatggccg taaagcccag ctccagcgcc agccggaaag ataccggccg cccgcaggcg
     2941 cggcaatggg cgctcaaaaa gagcgctgaa aactcgtagt aagacagcgc cgtcgccagc
     3001 gccgccagca gagcggcggc cagcaggccc aaaagatttg taaagatgga caggatattc
     3061 tcgttgaaga cgagccccct gccccccagg cccaacaggg tattggtcat ccaggacaga
     3121 gccggccgca gggccagcgc cgccagagct ttataaagga tttcgaacag cagcagcccc
     3181 ggcaggatgc cgagataggt ctgcagcacc agccgcagcg tctgcggcag gggccgggag
     3241 ggtgagtgca tggtccatct tcctctcact gggaattgtt ttggcccgcg cgggctgtag
     3301 gggaacgttt tttcggggca gtcgcgccgg gccctggata agttttttct cgaagtggct
     3361 gcgtcggtcc cctgagcggc gcttttctca aaggggctac atcggccccg tgagcggcgc
     3421 ttttctcgga gtggccgcgc cgggccttag gccaatgctt ttttcagggc ggtcgcgccg
     3481 agacttggcg ggcgtttttt tcaaagccgc agcgcccggc tcgggcggcg gaccgcaaaa
     3541 agggccgccg cgggtcccgt ctgtcccgcg gcggctcttt tggaatgggg ttcaaaacga
     3601 tcccgggcgc tcgtgtcgct ctcagccacg ctcaggccag ctcgaagtcc acattgcccg
     3661 ccgagatgga gacgccgatc aggcgcaccc gcaccgggtc gccgatgcgg tagctcttgc
     3721 cggtaaggct gtccaccagc gccatgctgt tggacaggtt catctcgccg tcggacaggc
     3781 ggctgacgtg caccaggccc tcgacggtgt tctccagctc cacatagatg ccgaaattgg
     3841 tgaccgagga gatggtgccc tcaaactccc cgccgatctt gtcggccata tactcggcct
     3901 tgtagcagtc gtcggcgctg cgctcggaca ccatggccag cacctcgcgc tcgctggtgt
     3961 ggcgcgcggc ctcaaccaca aactcgccgt atttgccggt gagctctttt tcagttgcgc
     4021 ccgccagggc cgccgagagg atgcggtgga cgatcaggtc cggatagcgg cggataggcg
     4081 aggtgaaatg ggtgtaatcc ggcagagcca ggccaaaatg ccccttgggc tcgctgagat
     4141 attttgcctt tgccatgctg cgcagcacat tgatgtgcac cgcccgctcg aggctggtgc
     4201 cgcgggcgtc gttgagcagc ttggacagct ccagctgggt gggggtctcg cccgcgaatc
     4261 tggcgtccag gcccatagcg gtcagcgcgt cgtgcagccg ctcgacccgg tccgggtccg
     4321 gcagctcgtg cacgcggtaa agaaacggca ctttcaattt tttgcccagc ttggcggcgc
     4381 tggtgttggc cagcagcata aactcctcaa tcatctgctc cgccacgccg cgctcgacct
     4441 ttttgacgtc cacggccttg ccgtccgcgt ccaggatgat ctttgcctcg ccggtctcga
     4501 tctccatgca gccgcgcgcc gcgcgccgct tggccagctt gtcgtagagc tcgcgcatca
     4561 gctccagcgt gggcaggcac tcggcgtatt tctgctgcag gtcctggccc gcaagccccg
     4621 cataaatgct gttgatctcg ctgtaaacgc cctttacgcg gctgcgcatg acggccttgt
     4681 aaaagctgaa atccgtcacc ttgccggtga catccaactc catcacacag gaaaaacaga
     4741 gcctgtccac gccgggattg agggagcaga taccgttgga gagctgccgc ggcagcatcg
     4801 gcaccacggc gcccggggtg tagacggagg tggcgcggtg atatgcctcg tcgtccagcg
     4861 ccgtgccggg caccacatag tggctcacgt ccgcgatgtg cacgcccagc tcataaccgg
     4921 tctcggtgcg cgccagggag atggcgtcgt cgatgtcctt ggtgtcagcg ctgtcgatgg
     4981 tgaagatgac ccggtcacgg aagtctttgc gccccttgag atcgccctgc ttcacctcgg
     5041 cgtcctccag gcgttttgcc tccgccttga ccttgcccgg gaacgacttg acgatgtcgt
     5101 tggcgtacag gatggccttg gtgcaggatt tggcgctgtc cgacgagccg aagcactggg
     5161 agatgcccac ccggtggtcc cggtggctct cgccgcgctc caatatttcg gccgccacct
     5221 tttcgcccac ctcggcgcct ccgtcggcgc tctttttgat cggcagcggc agggccgggc
     5281 atcggtcggg cagcagcacc aggcggccct cctgccggtc caccacgccc accagcttgt
     5341 tgttttcctc cacgatggcc accacctggc cctcgtcttc gctgcggccg cccaccagcc
     5401 gcttcaccag caccacgtcg ccgggcatgg cgcccagcag aaagcggccc ggcacaaaaa
     5461 agtcggcggg cttttcagcg ggcaggttgc cctgttcgtc ctcttccacc accggggcgg
     5521 cgaagccgaa gttcttggcc accttgacga cggtgcattt tattgcctcg ccggtggtcg
     5581 cgggctgggc agagcgcgcc ttgcggaaat agacgccgtc tttttccacc accaggccgt
     5641 ccttaaccag aaagcccaat accgcctgga cctttttgtc ctcgtcgaat ttggctttca
     5701 gctctttcag gcgccggggg cgcttttcaa gttctttcaa aattttagct ctcagtgaca
     5761 taaatccgtt ccttttctcc cttttagggt ttccggcctg caagccggat atactttcct
     5821 tccgccattc ttccggtgcg gcgggcggcg gtccgccgtc tcccctcccc gtcaaaaatc
     5881 ccgggccggg agggctggcc ctgcggggta tcccccgccg tttacagaaa aagcccgccg
     5941 aaagggtcgg cgggctttgc tggcaactat ttcgcgaata cgctgaacag gctcacggcc
     6001 agggtcacca caaaaaagac gatcgccgcg attttggtat acttcgccag aaccgcgtct
     6061 gtggtgcggg tgcgtgcctc ggaggtcatc gcctcgccgc cgccgatcgc gccggaaagg
     6121 ccgttgccgc tgggctgctg caccagaacc ataaaaataa tcacaaggct ggcgagaatc
     6181 agcagaacgc cgccaacga
//
LOCUS       DBMDYA010000066         6193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3223_length_6193_cov_1.524787, whole genome shotgun sequence.
ACCESSION   DBMDYA010000066 DBMDYA010000000
VERSION     DBMDYA010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3223_length_6193_cov_1.524787"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="ACZPFK_02660"
     CDS             complement(<1..1200)
                     /locus_tag="ACZPFK_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019941.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HIR1405244.1"
                     /translation="MKHLFRRLVCLSLALTMSGCIFFITALGAETAQAPAAPVQSESA
                     PALPSPTPAAPESDGVEPPKSPAGAEETPVPPVSPAPEATPTPEAAVPEASATPAPEE
                     DAPAAEPPQPSATPQALAFALSAAPAIDYPYTAEDPFPAPHMGGMIINGYHTYCVEED
                     RAWPSAGSAYGDGGELPVSAEVKAQLLRALEAGYPQDALGLSALQPGLSAQVLATYTQ
                     WVIWNILNPGHYDMGADAVYFQALRRYALTGEAETGRVDQVTVSAAAPILRYDSKSGL
                     FCAELSFTANKAALLTLGPLPAGLTVTLRSSGVALVEGSTFFSGDTLLLTAQSPSVTG
                     LFGTLSFSYVTYQALSGDIRLFVPQNSSQQKMLGYRLDPVTEALRVYFSVDPTTPQPT
                     ATPTATPT"
     gene            2301..3290
                     /gene="trpS"
                     /locus_tag="ACZPFK_02665"
     CDS             2301..3290
                     /gene="trpS"
                     /locus_tag="ACZPFK_02665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1405245.1"
                     /translation="MEQKKRILTGDRTTGKLHLGHYVGSLQNRVKLQDQYDTFILLAD
                     IQALTTHFENPALIRSSIYDVAIDNLSVGLDPEKATIVQQSQITSIAELTEIYSLLVT
                     VNTLRHNPTIKTEAAAYGYDDLTIGFLGYPVSQTADITFCGADLVPVGEDQRPHLELA
                     RKIVRRFNDLYGETLNEPEILLSDCPRLAGLDGNAKMGKSLGNAIYLSDDAATVEQKV
                     RGALTDKNRITAHDPGNPDVCVVSRYHEVFNKGEHEEICASCRAGSIGCVACKKRLAA
                     ALNALLDPFREKRAYYEAHMDEVKQIIQAGSDKANRVGNETMRRVKDAMQISL"
     gene            complement(3773..4447)
                     /locus_tag="ACZPFK_02670"
     CDS             complement(3773..4447)
                     /locus_tag="ACZPFK_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR1405246.1"
                     /translation="MKKKDRALALLRLIPLAVMLGLIAAVALSGHSLSAEEIFNYSPG
                     NPWLAAGFLLLLYALKSLSIVFPIVVLYLAGGMLFSTPAALAVGTLGAAVCLSLPYWV
                     GRWSAGSFAQTLARRYPKVRALLDLQRENDFFFSFFVRVIGFLPCDVVSLYLGSAGLP
                     FGPYLAGGLLGMLPGIFTVTLMGASIQDPSSPQFLISAAVTLLLSLGSMIVAHRARRG
                     KTGSGG"
     gene            complement(4900..6189)
                     /locus_tag="ACZPFK_02675"
     CDS             complement(4900..6189)
                     /locus_tag="ACZPFK_02675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1405247.1"
                     /translation="MFAVEYLPGQFDQRADSASECIQLISQGARPEVRSATVYVLEGA
                     LSDEDVAAIKRYVINPIEAREASLEPRTTLRMEQPVPADVEVLEGFLDLDEAGLAHEV
                     FRGAKPNPRLSHAREGVQRALAMKADFILAVGGGSTIDTAKGIAHGAANPGTDIWDFW
                     CGKAALERSTPVGVVLTISAAGSETSNSAVLTNEENGQKRGLGHDLNRPAFAVMDPEL
                     TLTLPVYQVACGVVDIMMHTLDRYFNPVWDNDLTDGIAEALLRTVIENGRRAVADKSD
                     YHAMSELMWCGSVSHNGLTGLGGKQDFAPHQLGHELSSRFDVAHGASLSAVWGAWAET
                     CLSAGPARFARLGREVWGLSGQDEAAARAAIQATVDYFRALGMPTCFSELGIGVQDEA
                     TLQEMADCVTYHGARTIGTFRVMGREEILAAYRLANR"
BASE COUNT         1175 a         1988 c         2108 g          922 t
ORIGIN      
        1 cgtgggggtt gccgtcggcg tggccgtggg ctgcggtgtg gtggggtcca ctgagaagta
       61 gacgcgcagc gcttcggtca ccgggtccag ccggtagccc agcatctttt gctggctgct
      121 gttttgaggg acaaagagac ggatgtcccc cgaaagcgcc tgataggtga cataagagaa
      181 agacagtgtg ccgaaaaggc cggtgacgga gggggactgc gcggtcagca gcagcgtgtc
      241 accgctgaaa aaggtgctgc cctcaaccag cgcgacgccg ctggagcgca gggtcacggt
      301 gagcccggcg ggcagaggcc ctagggtgag cagggcggcc ttgttggcgg taaaagagag
      361 ctcggcgcaa aaaaggccgc tttttgaatc atagcgcagg atcggcgcgg cggcggatac
      421 cgtcacctga tccacccggc cggtctccgc ttcgccggtg agggcgtagc gacgcagggc
      481 ctgaaaatag acggcgtcgg cccccatgtc gtagtgtccg gggttgagga tgttccagat
      541 gacccactgg gtataggtgg ccagcacctg ggcgctcagg ccgggctgca gggcgctcag
      601 ccccagcgcg tcctggggat accccgcctc cagggcgcgc agcagctgcg ctttgacctc
      661 ggcggagacc ggcagctcgc cgccgtcgcc gtaggcgctg cccgcggagg gccaggcccg
      721 gtcctcctcc acacaatagg tgtggtagcc gttgatgatc atgcccccca tgtgcggggc
      781 gggaaagggg tcctcggcgg tataggggta gtcgatggcc ggggcggcgg acaaagcgaa
      841 cgccagggcc tggggcgtgg cagagggctg cgggggttcg gcggcgggtg cgtcctcttc
      901 cggcgccggg gtggcggagg cctcagggac ggcggcctcg ggcgtcgggg tagcctccgg
      961 tgcaggcgaa accggaggta cgggggtctc ctcggcgccc gccgggctct ttgggggctc
     1021 caccccgtcg ctctctgggg cggcgggtgt gggcgagggc agggcgggcg cgctctcact
     1081 ttgtaccggg gcggccggag cctgcgccgt ctccgcaccc agcgcggtga taaaaaagat
     1141 gcagccggac atcgtcagcg ccaggctcag gcagacgagc ctgcgaaaaa gatgcttcat
     1201 ggatctcaaa ctccctttct gacgcggagg cgccgcaaaa tagaatgccg attaaaacga
     1261 taggaataga aaaacgcgat acatatcata gcataaatcg gtcaccaaaa agtcacagat
     1321 aagcatatta aagtaaaaga atcaaaatat aagcttcacg cgggcaacag gatcgcaccc
     1381 ggcggggcgc gcaggcaaaa aatggccgcg gggcattttg ggcgtgcggc catccctccg
     1441 gccgggggcc tggcggtcaa gcgcttttta ttgcaaagca ggaaaagcag ctgcggagag
     1501 cgatggcggc agggctggtt gtgaagaaag ccctgcgcct ttttgaggcg gacaagcggg
     1561 caaagacggc ccgctccgcc gtttcgtgac ggtgctccct gccgggggag cctgcggctg
     1621 ccgctgtggg cggcgggcgc ggaacacaag aggcgccgag ggccgcggct gcgctccagg
     1681 cgtgaagcac tgtcttcggc ggcgagcggc ggcggcgccg aggccgcggc gcaggggcta
     1741 aaaaagcgag aaccagagca aaaaatgtcg cctcggaggg gagccccctc agcccccgcg
     1801 cggcggcgca gcggggagaa aggcgccgtg cggcagggct ttgaaccgaa cgctgaaaga
     1861 gaaatgcggc gcaaaaacga gaagccccgg ctgcgcgaag ggctgagacg caccgttttt
     1921 acctgcccgg cgggcgctgc ggcgcggcgc gcttgacacc ggggcgcggc ggggctataa
     1981 ttgccctaag gtaaaccgtt gagtgagggg agtaggtccg cagggccgcc gccaaagaga
     2041 gctgccgcag ggtgtgagcg cagcgcgggg gcgcgggctg aatgggctca cgagggcggc
     2101 ccgaaccggc tgcggccgca gtaggcgccg acggcggccc ggccgttatc ccggggcgca
     2161 tattttaagg tatgcggcgg gccgcacggg tggcacacag agcaaaagcg cagcgcgccc
     2221 tctgtcccag gcagggacgg agggcgtttt tcttttccgc tccgctgggc ggcgtcaaca
     2281 cacaaaagga gagaaaaaac atggaacaga aaaaacgcat tttgaccggc gaccgcacca
     2341 ccggcaagct gcatctcggc cactacgtgg gcagcctgca gaaccgcgtc aagctgcagg
     2401 atcaatacga cacgttcatc ctgctggcgg acatccaggc cctgaccacg cactttgaaa
     2461 acccggccct catccgcagc agcatctacg acgtggccat cgacaacctg tcggtgggcc
     2521 tggacccgga gaaagccacc atcgtgcagc agagccagat cacctccatc gccgagctca
     2581 ccgagattta ttccctgctg gtgaccgtca acacgctgcg ccacaacccc accatcaaaa
     2641 ccgaggctgc ggcctacggc tacgatgacc tgaccatcgg ctttttgggc tacccggtca
     2701 gccagacggc ggacatcacg ttctgcgggg cggatctggt gccggtgggc gaggaccagc
     2761 gcccccatct ggagctggcc cgcaagatcg tgcgccgctt caacgacctg tacggcgaga
     2821 cgctgaacga gccggagatc ctgctcagcg actgcccgcg gctggcgggg ctggacggca
     2881 acgccaagat gggcaaaagc ctgggcaacg ccatctatct gtccgatgac gcggccacgg
     2941 tggaacaaaa ggtgcggggc gcgctgaccg acaaaaaccg catcaccgcc cacgaccccg
     3001 gcaacccgga cgtctgtgtg gtgagccgct accacgaggt cttcaataag ggcgagcacg
     3061 aggagatctg cgccagctgc cgcgccggca gcatcggctg cgtggcctgt aaaaaaaggc
     3121 tggccgcagc cctaaacgcc ctgctcgacc cgttccgcga gaagcgcgcc tactacgagg
     3181 cgcacatgga cgaggtcaag cagatcatcc aggcgggcag cgacaaggca aaccgggtgg
     3241 gcaacgagac gatgcgccgc gtcaaggacg ccatgcagat cagcctgtga cccgctcccc
     3301 ccggcgcgcg ggacggcccc gccacagcgg tgaggttggc ccacggggaa aaggccgggg
     3361 gctcccctgc cggcgggatc gccgggaagc gccaaaaaaa gagcttgacg ccccgttaag
     3421 ggcatcaagc tcttttttat tgctccgcaa taaaaacgac ttattcctat ggccgtggcc
     3481 cggggacggg ccacatggcc gaaaaaacag cctccgcttc gctgcggctc ttttttatcg
     3541 ctccgcaata aaaaaactta tcttaacggc ctcgtttctc gccgctgccg cggcaaccga
     3601 aacgcatggc ccaaaaaaca gcttgttttg acggccccgg ccaaggggct gaccccatgg
     3661 ttaaaaaata gcctcgccgc ggccatttct gagctgttaa aaaatggggg gccgcctcgg
     3721 gaagggcgat cccggcggcc gcttccccgc ttttaccctt tgccggtgaa actcatcccc
     3781 ccgagccggt cttgccgcgc cgggcgcggt gggcgacgat catggagccc agagacagca
     3841 gcagcgtcac cgccgcggag atgaggaact ggggcgagga ggggtcctgg atgctggcgc
     3901 ccatcagggt gacggtgaaa atgcccggca gcatccccag cagcccgccc gccagatagg
     3961 ggccgaaagg cagcccggca gagccgagat agaggctgac cacgtcgcag ggcaaaaagc
     4021 cgatcacccg cacaaaaaag gagaagaaaa agtcgttttc ccgctgcagg tccagcaggg
     4081 cgcgcacctt gggatagcgg cgcgccagcg tctgggcgaa gctgccggcg gaccagcgcc
     4141 ccacccaata gggcaggctg agacagaccg cggcgcccag cgtgcccacc gccagggccg
     4201 ccggggtgga gaacagcatc cctccggcca gatagagcac cacgatggga aagacgatgg
     4261 acaggctttt cagcgcatag agaagcagca ggaagccggc cgccaaccag ggattgcccg
     4321 gggaatagtt gaagatctcc tcggcgctga ggctgtggcc ggagagggcg accgcggcga
     4381 tcagccccag catcaccgcc agcgggatca gccgcagcag cgccagggcc cggtctttct
     4441 ttttcatgcg cttcccctcc tctccggggc gcctgccccg tttgaaaatc ttattgtacc
     4501 accgcgcaga gcaaacggca agtaaaaaga cgggccgcag cgctgcgttg gggcaggaaa
     4561 atatcggtta tcgagcggcg cttttgcact ttgcgtcgtt aaaaaggtgg acgatccgca
     4621 aagatcaccc accttttttc acaatgcttc ttctcaaagg gccggtgtaa aaccgctgcg
     4681 cgcccgcggg agaaagggcc gcgccgcgaa gcggagaaaa tccctgcgcc ttgcctgcgg
     4741 cgacgctggc ccgccctgct tttccgcggg gtgggcaagg cgcgcagccg cggccggctt
     4801 tggggccgcc gggccggttt tggcgccctc ctgcgcacag accccctctc ccccggacgc
     4861 tgcctttcgc ggcgatgcgg ccggggttcg gccgctctct cagcggtttg ccagacgata
     4921 ggcggcgagg atctcctccc ggcccatcac gcggaacgtg ccgatggtgc gcgcgccgtg
     4981 ataggtgacg cagtccgcca tctcctgcag cgtggcctcg tcctgcaccc cgatgcccag
     5041 ctcggaaaaa caggtcggca tccccagcgc gcggaaatag tccaccgtgg cctggatggc
     5101 ggcccgcgcc gcggcctcgt cctgcccgga caggccccag acctcccggc ccaggcgggc
     5161 gaagcgggcg gggccggcag acaggcaggt ctcggcccag gcgccccaca ccgccgagag
     5221 cgacgcgccg tgggccacgt cgaagcggga ggagagctcg tgccccaact ggtggggcgc
     5281 aaagtcctgc ttgcccccca ggccggtcag cccgttgtgg gacacgctgc cgcaccacat
     5341 cagctcgctc atggcgtggt agtcgctctt gtccgccacc gcgcggcggc cgttttcgat
     5401 caccgtgcgc aaaagggctt cggcgatgcc gtcggtcagg tcgttgtccc acaccgggtt
     5461 gaaatagcgg tccagcgtgt gcatcatgat gtccaccacg ccgcaggcca cctggtatac
     5521 cggcagggtc agggtcagct ccgggtccat gacggcaaag gcgggccggt tcaggtcatg
     5581 ccccaggccc cgcttctggc cgttttcctc attggtcagc accgccgagt tgctggtctc
     5641 gctgcccgcc gccgagatgg tgagcaccac ccccaccggc gtgctgcgct ccagcgccgc
     5701 cttgccgcac cagaagtccc agatatcggt gcccgggttg gcggcgccgt gggcgatgcc
     5761 cttggcggtg tcgatggtgc tgccgccgcc caccgccagg atgaaatccg ctttcatggc
     5821 cagggcgcgc tgcacgccct cccgcgcgtg ggagagccgg gggttgggct ttgcgccccg
     5881 aaacacctcg tgcgccagcc ccgcctcgtc cagatcgagg aagccttcaa gcacctccac
     5941 gtcggcgggc acgggctgct ccatccgcag cgtcgttcgc ggctcaagcg atgcctcgcg
     6001 ggcctcgata gggttgatga cgtagcgctt gatggcggcc acgtcctcgt cggaaagcgc
     6061 gccctccagc acgtacacgg tggcgctgcg cacctcggga cgcgcgccct ggctgatcag
     6121 ctgaatgcat tcgctcgcgc tgtcggcgcg ctgatcgaac tggccgggaa ggtactccac
     6181 cgcgaacacg acc
//
LOCUS       DBMDYA010000067         6170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3240_length_6170_cov_1.493327, whole genome shotgun sequence.
ACCESSION   DBMDYA010000067 DBMDYA010000000
VERSION     DBMDYA010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_6170_cov_1.493327"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="ACZPFK_02680"
     CDS             <1..609
                     /locus_tag="ACZPFK_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019151.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GmrSD restriction endonuclease domain-containing
                     protein"
                     /protein_id="HIR1405248.1"
                     /translation="FLFLQNFYFAYGVVCGGKSNVLDDIVTDYAKRIENEDASAGIID
                     FIKKLRTYYPPYPQFLTAFKIVGYSQKVKSYRTPAKKHDVQYILREFENYWQAKNSEL
                     SVQTFTIEHIGGDNGEESNCRIGNLLPLAEGVNKRIGDDLFANKISQYKKSHFISVKN
                     FVERYGTKTEWTERDIDGRAAHMAELAYNKIWSILGIFSEVD"
     gene            complement(897..1160)
                     /locus_tag="ACZPFK_02685"
     CDS             complement(897..1160)
                     /locus_tag="ACZPFK_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405249.1"
                     /translation="MLERWMESGTSPFEQLRIDTETYSRAVKITEEIEMELEKFDSIS
                     SKRVSALLEACGTQCSEAENAAYLQGLRDGAQLFGELAGILRP"
     assembly_gap    1451..1460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1707..2918
                     /locus_tag="ACZPFK_02690"
     CDS             1707..2918
                     /locus_tag="ACZPFK_02690"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1405250.1"
                     /translation="MSTLGKKTVDELDVKGKRVLVRVDFNVPMKDGAITNDNRIVAAL
                     PTIKKLVAEGGKVILCSHLGKPKNGPEAKFSLAPCAVRLSELLGQPVVFADDDQVAGE
                     KAHAAVAAMKDGDVVLLQNTRFRKEETKNEPAFSEELASLADVYVNDAFGSAHRAHCS
                     TAGVTDYIKDTAVGYLMGKEIEFLGNAVNSPVRPFVAILGGAKVADKLSVIDNLLTKV
                     DTLIIGGGMAYTFLKAQGMEVGSSLVDDEKLDYCKEMLKKAEEKGVKLLLPVDTAIAP
                     HFPDPIDAVIDVTYVDADKIPAHMMGLDIGPKTREAFAEAVKSAKTVVWNGPMGVFEN
                     PVLAEGTKAVARAMAETDATTVIGGGDSAAAVMQLGYADKMTHISTGGGASLEYLEGK
                     VLPGIACIADN"
     gene            3015..3782
                     /gene="tpiA"
                     /locus_tag="ACZPFK_02695"
     CDS             3015..3782
                     /gene="tpiA"
                     /locus_tag="ACZPFK_02695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1405251.1"
                     /translation="MNKAKRKAVIAGNWKMNKTAGEAAELISALIPEVAGADCEVVIC
                     TPFTDLPVAVSKTAGSNIAVGAQNVHFEKSGAFTGEISADMLTELGVEYVIIGHSERR
                     QYFAETDETVNKRLKAALAAGLKVILCVGESLAQREQGVTSELVALQTKIALGGVSAA
                     ELKNVIIAYEPIWAIGTGLTATAEQAEEVCKTIREVVASLYGKAEAEAFTIQYGGSMN
                     AKNADELLAMPDVDGGLIGGASLKAPDFAAIVKAASK"
     gene            4163..5677
                     /gene="gpmI"
                     /locus_tag="ACZPFK_02700"
     CDS             4163..5677
                     /gene="gpmI"
                     /locus_tag="ACZPFK_02700"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1405252.1"
                     /translation="MKPLVLCIMDGFGNNPSDYGNAIHAAKKPNLDRLFAECPHTLIG
                     ASGMDVGLPDGQMGNSEVGHTNIGAGRIVYQELTRITKSIGDGDFFENEALTAAMDNA
                     AQDGRALHLMGLLSDGGVHSHNTHLYGLLEMAKRKGVKEVFVHCFMDGRDVPPSSGKD
                     YVEQLQAKMAELGVGRIATVMGRYYAMDRDNRWDRVEKAYSAMVYGEGEQNDSAVDAM
                     AKSYADGVTDEFVVPTVIAGGARIKAGDSVIFFNFRPDRAREITRTLVDPEFKGFERK
                     NGLFPLTYVCFTQYDATMPGVLVAFHPQSLVNTFGEYISRLGKTQLRIAETEKYAHVT
                     FFFNGGKEEPYAGEDRALIKSPKVATYDLKPEMSAYEVCDEMVRRIESGKYDVIVLNF
                     ANCDMVGHTGVFEAAVKAVEAVDECVGRIVKALEGCGGALLLTADHGNADKMYEPDGS
                     PFTAHTTNPVPFLVYGVPGAKLREGGRLADIAPTMLKVMGLPQPEEMTGVSIIE"
     regulatory      5873..6042
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1348 a         1813 c         1833 g         1166 t
ORIGIN      
        1 tttttgtttt tgcaaaactt ttactttgcg tatggggtcg tctgcggtgg aaaatcaaat
       61 gtgctggatg atattgtcac tgattatgca aagcgaattg aaaatgaaga cgccagtgca
      121 ggaattatag attttattaa aaaacttagg acatattatc cgccctatcc tcagttcttg
      181 actgcattta agattgttgg atattctcaa aaggtgaagt catacagaac tccagctaaa
      241 aagcatgatg tacagtatat cttacgagaa tttgagaact attggcaagc taaaaatagc
      301 gaactcagcg tgcaaacatt taccatagaa catattgggg gtgacaacgg agaagaaagt
      361 aattgtagaa taggaaatct tcttccttta gcagagggag taaataaacg aattggcgat
      421 gatttgtttg ctaataaaat ttcccagtat aaaaagtccc attttatttc tgtgaaaaat
      481 tttgtagaac ggtacgggac aaagactgaa tggacagaac gggatattga tggccgagca
      541 gcacatatgg cagaacttgc atataacaaa atttggtcaa ttcttggcat tttttcagaa
      601 gttgattgat ttctctttta cataaaaagg tcgatgtcca aatataattt gggcatcgac
      661 ctttttaata gatgaatgcc ttgcttgctg tctgcaaacc attgatatca cggaattttt
      721 ttgcagattt ttcaatgtcg cttggacccc ttagggcgac aagttttccc acgtatttaa
      781 agcactctcc ccgatccctt tagaaagggc gttatacgct tgtgccgggg gcgcgaaaga
      841 gcgaccccgg gctgcgaggc ggagggcttt cgcgcggcca aaggcggccg cgcgatttat
      901 ggacggagta tcccggccag ctcgccgaac agttgcgcgc catcgcggag accttgcaga
      961 taagctgcgt tctcagcttc tgaacattga gtgccgcacg cttccaaaag tgcgcttacg
     1021 cgtttgctgg aaatggaatc aaacttttca agttccattt cgatctcctc tgtgattttc
     1081 acggctctgg aataggtttc cgtatcaatt ctcaactgtt caaaggggga ggttccgctt
     1141 tccatccatc tctccagcat caactgcttt tctttcttcc ttttttaaat ttttagaacc
     1201 cctaatgagt tcacttgcct cattacaata caaaataagt ctactatgaa cccagcttag
     1261 atttattttg gaggttgcat tgtggaggac aagaagcatt tgtctttgag gatcgacccg
     1321 gccaccctta aaaaattcag atatgtcgcc caggccagcg accgctccgc caattcacgg
     1381 ctgctctacc tggtgcgcag gaacatcagg gaatttgaaa aggagcacgg ggagatccag
     1441 acggacgaga nnnnnnnnnn ctaaaggatg gttgtccgcc cgccgcagcg cccctctcgc
     1501 gggcgtcttc atcaagagga ccagctttcc tgttttgtgc ccttttgctt ttgtccaaac
     1561 tgttaaaaaa ttaacgtttc gcctgtaaaa tggccgttta acgcttgcat gcggcggcaa
     1621 atcttattat aatagaaaag gtgaaaacca cgatcgaaag ggtacggaac aggaaagttt
     1681 gaaataagaa agggagtacg gtttttatga gcactcttgg aaaaaagaca gttgacgaac
     1741 tcgatgtaaa gggcaagcgc gtcctcgtcc gggtcgactt caacgtcccc atgaaagacg
     1801 gcgccatcac caacgacaac cgcatcgtcg cggccctgcc caccatcaaa aagctggtcg
     1861 cggagggcgg caaggtcatc ctctgcagcc acctgggcaa gcccaagaac ggccccgagg
     1921 ccaagttcag cctggctccc tgcgcggtgc gcctgtccga gctgctgggc cagcccgtcg
     1981 tctttgccga cgacgaccag gtggccggtg aaaaggccca tgccgctgtc gcggccatga
     2041 aagacggcga cgtggtgctg ctgcagaaca cccgtttccg caaagaggag accaaaaacg
     2101 agcccgcgtt cagcgaggag ctggccagcc tggccgacgt gtatgtcaac gacgcgttcg
     2161 gctccgctca ccgcgcccac tgctccaccg ccggcgtcac cgattacatc aaggacaccg
     2221 ccgtgggcta cctgatgggc aaggagatcg agtttttggg caacgccgtg aacagcccgg
     2281 tgcgcccgtt cgtcgccatt ctgggcggcg cgaaggtcgc cgacaagctg agcgtcatcg
     2341 acaacctgct gaccaaggtg gacaccctga tcatcggcgg cggcatggct tacactttcc
     2401 tgaaagcgca gggcatggag gtcggctcct cgctggtgga cgacgagaag ctggactact
     2461 gcaaggagat gctgaaaaag gccgaggaga agggcgtcaa gctgctgctg ccggtggaca
     2521 ccgccatcgc gccgcacttc cccgacccca tcgacgcggt catcgacgtc acctatgtgg
     2581 acgccgacaa gatccccgcg cacatgatgg gcctcgacat cggccccaag acccgcgagg
     2641 cgtttgccga ggcggtcaag agcgccaaga ccgtggtctg gaacggcccc atgggcgtgt
     2701 ttgagaaccc ggtgctggcc gagggcacca aggctgtcgc cagagcgatg gccgagaccg
     2761 acgccaccac cgtcatcggc ggcggcgact ccgcggctgc ggtcatgcag ctgggctatg
     2821 cggacaagat gacccatatc tccaccggcg gcggcgcctc gctggagtat ctggagggca
     2881 aggtcctgcc cggcatcgcc tgcatcgcgg acaactgaaa atagagatgc ggcggcctgg
     2941 gccgccgcat ttttagcagg ctgcccgccg gcttgcgggc ggccgtcaca caccacacaa
     3001 aggagaggtt ctcaatgaat aaagcaaagc gcaaggccgt catcgccggc aactggaaga
     3061 tgaacaagac cgcgggcgag gccgccgagc tgatctccgc cctgatcccc gaggtggcag
     3121 gcgccgactg cgaggtcgtc atctgcaccc cgttcaccga cctgccggtc gccgtctcca
     3181 agaccgcggg cagcaacatc gccgtgggcg cgcagaacgt ccactttgaa aaatccggcg
     3241 cgttcaccgg cgagatctcg gccgacatgc tcaccgagct gggcgtggag tatgtcatca
     3301 tcggccactc ggagcgccgc cagtactttg ccgaaaccga cgagacggtc aacaagcggc
     3361 tgaaagcggc cctcgcggcg ggcctgaaag tcatcctctg cgtgggcgag agcctggccc
     3421 agcgcgagca gggcgtcacc agcgagctgg tggccctgca gaccaagatc gccctgggcg
     3481 gcgtgtcggc ggcggagctg aaaaacgtca tcatcgccta tgagcccatc tgggccatcg
     3541 gcaccgggct gaccgccacc gccgagcagg cggaggaggt ctgcaagacc atccgcgagg
     3601 tggtcgcctc cctgtacggc aaggccgagg ccgaggcgtt caccatccag tatggcggca
     3661 gcatgaacgc caaaaacgcc gacgagctgc tggccatgcc ggatgtggac ggcggcctga
     3721 tcggcggggc ctccctcaag gcgccggact ttgctgccat cgtcaaggct gccagcaagt
     3781 gagcggccgg cgggcccggc ccgcccggct ctgaaaaaaa caggcgcaga cgcgcgttgc
     3841 gcacaggctt gcgcctgttt tgcttttgcg gcccaggccc gcgcggggcg tctgccgccg
     3901 cggcctgcgg cccatcttgc ggcctgcggc ctggcctgtg acgcccggat ccgtccggcg
     3961 cagcctgagc ggcccggcgg caggctctcc gccccccggc gcggctttcg catcccccgc
     4021 cgcggcctgc ggcgcggccc cctgaaccgg gcggcggacc gcaggcccga cggcgctgcg
     4081 cctgcgcccc ggcggccggc cccgcacccg agcgcagcgg cacgccgctg tttcatcccg
     4141 ctaaacaaaa aggagaaatt caatgaaacc tctggttctt tgcatcatgg acggcttcgg
     4201 caacaacccc agcgactacg gcaatgcgat ccacgcggcg aaaaagccga acctggaccg
     4261 gctgtttgcc gagtgccccc acaccctcat cggcgcctcc ggcatggacg tgggcctgcc
     4321 cgacggccag atgggcaaca gcgaggtggg ccacaccaac atcggcgcgg gccgcatcgt
     4381 ctatcaggag ctcacccgca tcaccaagag catcggggac ggcgactttt ttgagaacga
     4441 ggccctgact gcggccatgg acaacgcggc gcaggacggc cgcgccctgc atctgatggg
     4501 ccttttgtcc gacggcggcg tgcacagcca caacacccac ctctacggcc tgctggagat
     4561 ggcgaagcgc aagggcgtga aagaggtctt cgtccactgc ttcatggacg gccgcgacgt
     4621 gcccccctcc tccggcaagg attatgtgga gcagctgcag gccaagatgg ccgagctggg
     4681 cgtgggccgc atcgccaccg tcatgggccg ctattacgcc atggaccgcg acaaccgctg
     4741 ggaccgggtg gaaaaagcct acagcgccat ggtctacggc gagggcgagc agaacgacag
     4801 cgccgtggac gccatggcca agagctacgc cgacggggtc accgacgagt tcgtggtgcc
     4861 caccgtcatc gccggcggcg ccaggatcaa ggcgggcgac agcgtgatct tcttcaactt
     4921 ccgccccgac cgcgcccgcg agatcacccg taccctggtg gaccccgagt tcaagggctt
     4981 tgagcggaaa aacggcctct tcccgctgac ctatgtctgc ttcacccagt acgacgccac
     5041 catgccgggc gttctggtgg cgtttcaccc gcaaagcctg gtgaacacgt tcggcgagta
     5101 tatctcgcgc ctgggcaaga cacagctgcg catcgccgag accgaaaagt acgcccacgt
     5161 cacgttcttc ttcaacggcg gcaaggagga gccctatgcg ggcgaggacc gcgccctcat
     5221 caagagcccg aaggtggcca cctatgacct gaagccggag atgagcgcct atgaggtctg
     5281 cgacgagatg gtgcggcgca tcgagagcgg caagtacgat gtgatcgtgc tcaactttgc
     5341 caactgcgac atggtgggcc acaccggcgt cttcgaggcg gcggtcaagg ccgtcgaggc
     5401 ggtggacgag tgcgtgggcc gcatcgtgaa agcgctggag ggctgcggcg gcgcgctgct
     5461 gctgaccgcc gaccacggca acgccgacaa gatgtacgag ccggacggca gcccctttac
     5521 cgcccacacc accaacccgg tgcctttcct ggtgtacggc gtgcccggcg ccaagctgcg
     5581 cgagggcggc cgtttggcgg atatcgcccc caccatgctc aaggtgatgg gcctgcccca
     5641 gccggaggag atgaccggcg tcagcatcat cgagtgagct ttccgtttaa aaacaggcgg
     5701 cgggcgcccc ggtcatccgg gcgcccgccg ctcttttttg ttttttgggg gcgttgcttg
     5761 tctgttttcg tctgcttttc gcgcaaaatg gggccggatt ttggcaatgt aacaaaaagt
     5821 tcaaaaatgc tggacagcgg cgggggcttt gtgctatagt gaagtcacga aaaaagttga
     5881 tagaggcgcg gcgcggatga gtaaccggca gagggggcac cctgagaagc cggcaaaagg
     5941 ccaaaccgcc gaaacggacc gccgggcacg gcggctcagt tgggcatggg gagaagatct
     6001 ccatgactcc tgcccgatag gggcagagga gctatcggtg ataactgacg cccctttcgc
     6061 gggtccggga gttgcatcga tggatgcaac ttttttttgt gcaaaaacct ggaaagggga
     6121 aagatcacat gaacgaaaat ccaaacacca ccgtacagga gctctcatcc
//
LOCUS       DBMDYA010000068         6158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3249_length_6158_cov_1.526498, whole genome shotgun sequence.
ACCESSION   DBMDYA010000068 DBMDYA010000000
VERSION     DBMDYA010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_6158_cov_1.526498"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="ACZPFK_02705"
     CDS             <1..406
                     /locus_tag="ACZPFK_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1405253.1"
                     /translation="SLVRQVDTMKRKQFIKGTTQIANEGAIQVFQDLGGGMEEVVVGV
                     VGVLQFEVFEYRLKNEYNVDVHMQRMPHQFIRWIENEDLDPNTLSLTSDTKKVQDVRG
                     GKLLLFTNSWSIQWALDHNEGLVLSEFGKAKV"
     gene            complement(1433..1918)
                     /locus_tag="ACZPFK_02710"
     CDS             complement(1433..1918)
                     /locus_tag="ACZPFK_02710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1405254.1"
                     /translation="MIRIRPYKAADANTVLSWCQDEIAFYKWTAGVLGNYPITQNEFR
                     FVESFMPFTAFDETGIVGFFTLRNPNESLDELRFGFVIVNPEKRGKSYGKAMLRLGLK
                     FVFEIYGAKRATLGVFENNLPAYYCYKAVGFDDVILDTTETYCVLGEEWKCKELMMEN
                     V"
     gene            complement(2268..2747)
                     /locus_tag="ACZPFK_02715"
     CDS             complement(2268..2747)
                     /locus_tag="ACZPFK_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1405255.1"
                     /translation="MIRPARREELETCAAVVRESFATVAPQYGITRESAPRHTSFLSA
                     DRLREQYARGEPLYVYELRGRIVGYCSLSPGSGPHCRELNHLAVLPACRHQGVGRALV
                     QHAVQQARALGCTRLEIGIIEENTLLKSWYLKMGFVPTGTEKFDFFPFTCGYLELAL"
     gene            complement(2778..3965)
                     /locus_tag="ACZPFK_02720"
     CDS             complement(2778..3965)
                     /locus_tag="ACZPFK_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1405256.1"
                     /translation="MELIRMQMLGGFAVSVGEVEICGPNCRKGQLWNLLQYLVTFRHR
                     QIPASELQAALWPKGTGNPSNALKNLVYRVRTTFEDAGIADAKQIILCKRGVYRFNAD
                     LPLEADTELFAAAAAQALEAEDEQRIPLLKKAIALYRGDYLTGEERQSWVAAVRADLH
                     EKYAACALPLMEHLNAEQDWDALLHLATHSTDIDPFDAPANYYQLQAMLGLGMQPKAL
                     EYYNYLQNLYYRERGESLPEQVRSLYHDIIRHMNNIEIDIAKIKQDISEKGEARGAFL
                     CEYEVFKEMYRVEARAAARTGQKMFLGLLTLTDEEGAPPAGRARNRAMDHLEKTLVLS
                     LRRGDVVARFSVTQYVVLMATRTYENGLKVINRVERRFRAEYRGRGVRLATTLQPIDT
                     GEE"
     gene            4297..4737
                     /locus_tag="ACZPFK_02725"
     CDS             4297..4737
                     /locus_tag="ACZPFK_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1405257.1"
                     /translation="MKKLIAIALSLVLALGLVACGGAKVQDGTYTAELSDAAAEANHG
                     WKDYLTITYKDGKIAELDFDSKDADGNLKSEWATPENYPMTPQPSEWIPQLEENLKAT
                     TNPDKVAAVAGATLGSNHAKALYKAVIEKAQAGDATVAVVDVGE"
BASE COUNT         1297 a         1790 c         1839 g         1232 t
ORIGIN      
        1 ctccctggtg cgccaggtgg acaccatgaa gcgcaagcag ttcatcaagg gcaccaccca
       61 gatcgccaac gagggcgcca tccaggtctt tcaggacctg ggcggcggta tggaggaggt
      121 cgtggtgggc gtcgtgggcg tgctgcagtt cgaggtgttc gaataccggc tcaaaaacga
      181 gtacaatgtg gacgtgcaca tgcagcggat gccccaccag ttcatccgct ggatcgagaa
      241 cgaggacctg gacccgaaca ccctgtcgct gaccagcgac accaaaaagg tgcaggacgt
      301 gcggggcggc aagctgctgc tgttcaccaa cagctggagc atccagtggg cgctggacca
      361 caacgagggg ctggtgctgt ccgagttcgg caaggcgaaa gtctgacccc gccgcgcggg
      421 aggcgaaggg ggccaaagag acgaatgctt tgagtggccg ctgcggcggc cgcttttttt
      481 attgcggagc aaatttttac ggccgtggac gggggacggc cggatggccg aaaaaacagc
      541 tatttcaagt gtcaaaaagc ggcaggtcgg ttcataagaa cacctcgcaa acccgcatga
      601 aatgggcgtt tctccaatcc ggcgaataaa ccgacgcaac ctgcaatcgc atgattttgt
      661 aaagttgaca ccatttgggc accacaaaac aaaaaaacag caagcccttg tgcggcctgc
      721 tgacggactt ggcatacagc atatgatctg aaaaagaaat ccccgctgtt gacggtatag
      781 gacagacgcg gggcaaacaa gctttgcgaa aagaacatta ggtccttaaa gcctcgacag
      841 aatgggcctt tgtggatgcg gaagcaaagg gcctgtatga tttatcccat ccccctgaaa
      901 caggcttcca acattccgca caacccgccc cggcgtgaga aaagtatttt cttctgccgg
      961 gacgattttc atgaacggca ggaatcccga ttccaccgaa aacaagcgtg gcagacagga
     1021 cggagtatat acactcctgc aaaatgctgt tgggttctgg aacccaagcc ctggaaaagc
     1081 gccgctgccg gcgacctttg ctatcgtcct tgacgggcca gccgcacgtc ctgcggacgc
     1141 ttccagacgc aagaagctat gtaatttcca gccagtaagc tccccgtatt cagcgcccga
     1201 cagggcgcgt gggctccacc tgtggagtgt tcgcaaggca gagcaagcgg cccctgccgg
     1261 gggatggggg tcccagcaag tagattttcc aggctttcgc gcgggcgaaa aacgggaaaa
     1321 ccgcaaagtg tgaaccgctt tgccctgctg tcgctattga aaacggctaa agaggaagcg
     1381 cgtcttatac caataaggtt aaggcgcagg aaaccagttg acaaactgga atttatacat
     1441 tttccatcat cagttctttg cacttccatt cttcgcctaa aacgcaatat gtttcagttg
     1501 tatcaagaat cacatcatca aaaccaactg ctttatagca ataataagca ggaagattgt
     1561 tttcaaaaac tcctaacgtt gctcttttag ccccatatat ctcaaacaca aactttagcc
     1621 ccaatcgaag catcgctttc ccatagcttt tcccgcgttt ttcgggattt acaataacaa
     1681 acccaaaacg caattcgtcc aatgattcat tcggatttct taacgtaaaa aaaccaacga
     1741 ttcctgtttc atcaaatgca gtaaacggca tgaaagattc aacgaaacga aattcatttt
     1801 gtgttatagg ataatttcca agtacacctg ccgtccactt ataaaatgct atttcatctt
     1861 gacaccacga caaaaccgtg tttgcgtccg cagctttata gggtcttatt ctaatcatat
     1921 tttcccctcg aaaattctaa tttgtacgtc ttttaagccg aaatcgtgca accccctagc
     1981 tacttttttc attttacatg accgaatgga aatgtcaaac aactcatttt gtttcattac
     2041 ttcatgtaac tgctcgtaat tcggtttatt cgcttgggcc ccattcggcc accattttac
     2101 cctttgcaga acggtttctc tcatggcaga aatgctgact tcatcggcct ttccggccgg
     2161 gaaaggcccc gcccgcgcgg ctcgcactct gctttggccc cggcgcggag ctgctttttt
     2221 tcgcacgcgc agcctgccgg ggacggcaaa agccctctga gcacagctca gagggccaat
     2281 tccagataac cgcaggtaaa ggggaaaaag tcgaactttt ccgtcccggt gggcacaaag
     2341 cccattttca gataccagct tttcaaaagc gtattttctt cgatgatgcc gatctccaag
     2401 cgggtgcagc ccagggcgcg ggcctgttgt acggcatgtt gcaccagggc gcggccgacg
     2461 ccctggtgac ggcaggcggg caggacggcc aggtggttca gctcacggca atgcggcccg
     2521 ctgccgggcg acagggagca gtagccgacg atgcggcccc gcagctcata cacatagagc
     2581 ggctcgccgc gggcgtattg ctcccgcagg cggtcggcgg acaaaaaaga ggtgtgccgg
     2641 ggcgcgcttt cacgggtgat gccgtactgg ggcgcaacgg tggcaaagct ctcgcgcacc
     2701 accgccgcgc aggtctccag ctcctcccgc cgggcggggc gtatcatggg cagaactcct
     2761 ctcggggcgc aggagggcta ttcctcgccg gtgtcgatgg gctgcagggt ggtcgccagg
     2821 cgcacgccgc ggccgcggta ttcggcgcgg aagcgccgct ccacccggtt gatgaccttg
     2881 agcccgtttt cataggtgcg ggtcgccatc agcaccacat attgggtgac ggaaaagcgg
     2941 gccaccacgt cgccgcggcg caggctgagc accagggtct tttccaggtg gtccatggcc
     3001 cggttgcggg cgcgccccgc cggcggggcc ccctcctcgt cggtgagggt cagcaggccg
     3061 agaaacatct tctggccggt gcgggcggcc gcccgcgcct cgacccggta catctcctta
     3121 aagacctcgt actcgcagag aaaagcgccc cgggcctcgc ccttttcgct gatgtcctgc
     3181 ttgatcttgg cgatgtcgat ctcgatgttg ttcatgtgcc ggatgatgtc gtggtacagg
     3241 ctgcgcacct gctcgggcag gctctcgccc cgctcacggt aatagaggtt ctgcagatag
     3301 ttgtagtact ccagcgcctt gggctgcatg cccagcccaa gcatggcctg cagctgataa
     3361 tagttggccg gggcgtcgaa cgggtcgatg tcggtgctgt gggtggcaag atgcagcagc
     3421 gcgtcccagt cctgctcggc gttgagatgc tccatcagcg gcagggcgca ggcggcgtat
     3481 ttttcgtgca ggtcggcgcg cacggcggcg acccagctct gccgctcctc cccggtcaaa
     3541 tagtccccgc ggtagagggc gatggccttt ttcagcaggg ggatgcgctg ttcgtcctcc
     3601 gcctccagcg cctgcgcggc ggcggcggcg aacagctcgg tgtccgcctc cagcggcagg
     3661 tcagcgttga accggtagac gccccgcttg cagaggatga tctgcttggc gtccgcgatg
     3721 cccgcgtcct caaaggtggt gcgcacccgg taaaccaggt ttttcagggc gttggagggg
     3781 ttgccggtgc cttttggcca gagggcggcc tgaagctcgg acgccgggat ctgccggtga
     3841 cggaaagtaa cgagatattg cagcaggttc cagagctggc ccttgcggca gttggggccg
     3901 cagatctcca cctcgccgac cgaaactgca aaaccgccga gcatctgcat tctgatcagt
     3961 tccatggctg gtttctcctt tctgcccaaa gagcgaaaaa tgccgcgccg ccgcagcccg
     4021 cggcggttct ttgcggttta attatagaat aatccggcat taattacaat aaaaagtgac
     4081 cttttgacac gaattttttg catttggcca ccccgcgcgc gccgcgggga gtgaagcggc
     4141 ccccaaaagg gcatactgag gacagaggcg gcgcgcaccg tcaaaaacct gtgcaaaagc
     4201 aattgacaaa gtttgcctgt acatggtaaa atgaaatccc ataagacctt taggaaaata
     4261 gggaattgaa aagaaaaatc acaaggaggc attttcatga aaaagttgat cgcgatcgcc
     4321 ctttcgctgg tgctggcgct gggcctggtc gcctgcggcg gcgccaaggt acaggacggc
     4381 acctacaccg ccgagctgag cgacgccgcc gccgaggcga accacggctg gaaagattac
     4441 ctcaccatca cctacaagga cggcaagatt gcagagctgg acttcgactc caaggatgcg
     4501 gacggcaacc tgaagagcga gtgggccacg ccggagaatt accccatgac cccgcagccc
     4561 tctgagtgga tcccgcagct ggaggagaac ctcaaggcta ccaccaaccc cgacaaggtg
     4621 gcggcggtcg cgggcgcgac gctgggcagc aaccacgcca aggcgctgta caaggccgtg
     4681 atcgaaaagg cccaggcggg cgacgccacc gtggcggtcg tggacgtggg cgaataaggc
     4741 caaaggcggc cgcatccgcc tgcctgtggc ggcagactgc cggctccatc ggcgggtaca
     4801 cctgcctgtg gcggctcggc ctgtccgtga gcagagagcg cattttgtgt cggcagcgcg
     4861 tctttgaacc aacaaaaagt ttaaagcacg ggcttcaata cgcaccgtat tgaagcccgt
     4921 gcttttttat ctgctgctga acgcttgcgg cgctgccgaa agaagcccat gtaaaagcgg
     4981 ctgcttttcg cggggagcgg cgcggcccca tgccgccaag ctttcgatct gggaatggtg
     5041 ccgtcaggac gccagcgccc ccttggggga gatagggcaa atgaggtgga actcaatgag
     5101 agattcggca ggtctggggg ctatgtcttg gaactgctga caaagccgca ccttgaaaat
     5161 attggcgctt tcctccggcc tatttcacaa caatgcccgt taagccgcaa aggcttgtct
     5221 gcgttttttg tttcatgcgc cgaaaaaatc gcctgcgttt tctgcggccc ttggtttgtc
     5281 aacagtcccg tctcgagacg cctatcggta aaaggcccga ccggggcaac tcccactacc
     5341 ggtttggccg tcggtggtgt ctgtgttgaa agctgccggc gccgccgaaa gcggcgtccg
     5401 cgaaaaaacg tcggattgcc tccttgaggg ccgcgggggg ggggatctgt gaggggaatc
     5461 cccagaagca aagatcaaag cgggcgaagc gggcggagaa gctgcccgtt taggggcggc
     5521 ggggcggctg atgcgaagaa gaggagcccc agtgcgctct gaggcgctca ccggcggcct
     5581 cgcacggcct tgcccttttt aagcgcgctc gtgcgtatcc tcgtttccag ctcaccggcg
     5641 gcccctgcgg gatctgcccc tggcgacggc gaaaagatcg ggagaagcgt ccccaagctc
     5701 aacggcagcc ccccgcgcgg cctgcccctg ccgtcgggcg gtggttggga agggggagcg
     5761 gcggcccggc ccgtttggcc tttttaacgc ggctccggtg ggccgcaggc gaccgctccc
     5821 gacggaaaaa cgtctcctgt gtccagatat cacgtgcagg gcatgcggcc ttgcatgcgg
     5881 cgctttcagt ccgccgggtc tttggtcggt taccgaggct gcacaggcca gcccggtcgg
     5941 ccaaaaccgc ggcccctccg cccgcaccgc gctttgcccc ggccggtgct gtttgagttg
     6001 gcacaccgta agacgccggt tttccatccc tttgcgggtg tgcccggcct gttcgtccga
     6061 aaggggaggc tccgttcccc gggctgttga tttttgcgcc ggacgggccc tgcgggggcg
     6121 ggtctcagaa gacggccacc gtgtattcca gcgtgcgg
//
LOCUS       DBMDYA010000069         6143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3260_length_6143_cov_1.898709, whole genome shotgun sequence.
ACCESSION   DBMDYA010000069 DBMDYA010000000
VERSION     DBMDYA010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_6143_cov_1.898709"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="ACZPFK_02730"
     CDS             complement(<1..346)
                     /locus_tag="ACZPFK_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1405258.1"
                     /translation="MLDIKFVRENPELVKENIKKKFQDQKLPLVDEVIALDVENRKTI
                     TEAEALRAERNRLSKTIGGLMAQGKRDEAEQVKAQVARQADRLKELEAREAELDEKVR
                     GIMMNIPNIIDPS"
     gene            complement(346..1212)
                     /locus_tag="ACZPFK_02735"
     CDS             complement(346..1212)
                     /locus_tag="ACZPFK_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1405259.1"
                     /translation="MAAKKGGLGRGLDSLFAESAAGQGSKPSSLPLAEIEPDKNQPRK
                     HFEPESLSELAESIRLHGVLQPIAVRPNPAGGYQIIAGERRWRAAREAGLTEIPAIVK
                     EVGDEDAMELALIENLQRENLNPVEEAFGYQQLIEACNLTQEQAAAKLSKSRPAVANA
                     LRLLALPEGVLHLLRQGALSAGHARAVLTLEGDEARTDAAKLIVSQNLNVRQAEALCK
                     KMAKPKKERKAPPRPALPCEVELSLQEVLGTEVGVKYKEGKGTLQVHFYSDEQLKAFA
                     NLLGKYEKENGK"
     gene            complement(1325..2098)
                     /locus_tag="ACZPFK_02740"
     CDS             complement(1325..2098)
                     /locus_tag="ACZPFK_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1405260.1"
                     /translation="MSKVIAIANQKGGVGKTTTAVNLASAVAAAGKRVLLVDLDPQGN
                     STSGYGIPKRSVKNSVYQLLIGEGDPSSCVLHTKYRVDLIPSNIQLSGAGIELVALDR
                     RESRLREGIAQLAQKYDFIFIDCPPSLDLLTLNGLCACDTVLVPIQCEFFALEGLSEL
                     MSTVRTVKNMYNRYLEIEGVLLTMYDGRLNLTLQVVAEVKKYFPNKVYKTVVPRNVRL
                     SEAPSFGAPVNYYDPASKGAAAYRDLAAELLANNRPTGA"
     gene            complement(2835..3641)
                     /locus_tag="ACZPFK_02745"
     CDS             complement(2835..3641)
                     /locus_tag="ACZPFK_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1405261.1"
                     /translation="MFLSRLQVTGQVVLVPLDQIEPSPWQARRRFDPREMQALATSIR
                     ENGMLQPVTLQQLGKNRYALVAGERRVRAAQMLGMEKVPAILTDYEDGRAAVLSLEEN
                     LQRQQLGPFEEAEALRQLLTLWNCTQTEGAKRLGLSQSALANKLRLLSVAPEVRRAAE
                     EAELTERHVRALLRLESNAQQLQAVEKVRSEQLTVSATERLVERMGKEKRPKPKRRGM
                     VRDVRIFVNTVNRAVDMMKSSGIPASMERSETGEYIQYVVRIPTNKATLH"
     gene            complement(3815..4519)
                     /gene="rsmG"
                     /locus_tag="ACZPFK_02750"
     CDS             complement(3815..4519)
                     /gene="rsmG"
                     /locus_tag="ACZPFK_02750"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1405262.1"
                     /translation="MINMERFTANAARCGVPVTAEQAALLDRYAALLVEYNEKVNLTA
                     ITDPQGIEDKHFIDSLLLAAQPEAQGRLVDVGTGAGFPGVVAKLYRPELRLTLLEPTG
                     KRLDFLRWACGELGVEAEFLKERAEEAARKQWRERFDTATARAVARLNKLAEYCLPLV
                     RVGGYFLAMKAESAAEELQEARPAIERLGGEVVGLRRFSLPEGDERGIVIVKKISQTP
                     TVYPRNGGKIAKSPLK"
     gene            complement(4519..>6143)
                     /gene="mnmG"
                     /locus_tag="ACZPFK_02755"
     CDS             complement(4519..>6143)
                     /gene="mnmG"
                     /locus_tag="ACZPFK_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1405263.1"
                     /translation="GPAVHSLRVQSDRQRYHEYMKRLLERTDNLELKQGEVVRLLSEG
                     GRVTGVETRLCAGYSAKAVILATGTYLAGKIYVGEASYESGPDGQHAALGLSDSLRAL
                     GVSLRRFKTGTPARANRRSIDFSALEVQEGDEPVEPFSFMTDGELHNKVVCHITYTNE
                     QTHRIILDNIGRSPLYGGKIEGIGPRYCPSIEDKVMRFKDKPRHQIFIEPCGEDTDEM
                     YLQGMSSSLPEEVQNAFYHTIRGLEQLEIMRPAYAIEYDCCDPLELDASLAFKQIPGL
                     FGAGQFNGTSGYEEAAAQGLVAGVNAARSVQGKEPVILERQSSYIGTLIDDLVTKGVL
                     DPYRMMTSRSEYRLTLRQDNADERLTPLGRELGLVDDARWARFQADQAAKCRELDRLA
                     ARVLRPAEANPLLEKLGEPPLVTGATALELLRRPAVGYAALTGVIGPGEKVGAHVAGQ
                     IETEVKYEGYIRRQQQKIEALARQEKVKIPPELDYGPIEGIRLEAREKLSRVRPKNLG
                     QAARIPGVNPSDIAVLSVELAARRARDGKGRN"
BASE COUNT          990 a         2109 c         1720 g         1324 t
ORIGIN      
        1 cgctggggtc gatgatgttg gggatgttca tcatgatgcc gcggaccttt tcatccagct
       61 ccgcctcgcg ggcctccagc tctttcaacc ggtccgcctg cctggccacc tgggccttta
      121 cctgctcggc ctcatcccgc ttgccctggg ccatcaggcc gccgatggtc ttggacagcc
      181 ggttgcgctc ggcgcgcagc gcctccgcct ccgtgatggt cttgcggttc tccacatcca
      241 gggcgattac ctcgtccacc agcggcagct tctgatcctg aaacttcttt ttgatgtttt
      301 ccttgacgag ctcgggattt tcccgcacaa atttgatgtc cagcattatt ttccgttctc
      361 cttctcgtat tttcccagca gattggcaaa cgccttcagc tgctcatcac tgtaaaaatg
      421 cacctgcaag gtgcccttgc cctctttata tttcacgccc acctcggtgc ccagaacctc
      481 ctgcagcgag agctccacct cgcagggcag ggcgggacgg ggcggggcct tgcgctcctt
      541 tttgggcttg gccatctttt tgcagagcgc ttcggcctgg cgcacgttca aattctggct
      601 cacgatcagc tttgccgcgt cggtgcgcgc ctcgtctccc tccagcgtca gcaccgcccg
      661 cgcgtgcccg gcgctgagcg ccccctggcg cagcagatgc agcaccccct ccggcagggc
      721 cagcagccgc agggcattgg ccaccgccgg gcggcttttg gacagctttg ccgccgcctg
      781 ctcctgggtc aggttacagg cctcgatcaa ctgctgatag ccgaacgcct cctctaccgg
      841 gttcaggttt tcccgctgca ggttttcgat cagcgccagc tccatggcgt cctcgtcgcc
      901 gacctccttg acgatcgccg gtatctcggt gaggcccgcc tcccttgccg cccgccagcg
      961 gcgctcgccc gcgatgatct ggtagccgcc ggcggggttg ggccgcaccg cgatgggctg
     1021 cagcaccccg tgcagccgga tcgactccgc cagctcggac aggctctcgg gctcaaaatg
     1081 cttgcgcggc tggtttttat ccggttctat ctccgcaagg ggcaggctgg agggcttgct
     1141 cccctgccct gccgccgact cggcaaaaag ggaatccaac cccctgccga gccctccttt
     1201 ttttgccgcc ataggcgttc ctccttgtgc cgggcgcagt gctgcggccc gcgcgtttcc
     1261 gttttgtttt tctgccgcgc cttttcacac cgcgcggccc gattttttta cagctgtttt
     1321 ttgattatgc gccggtcggc cggttgtttg ccagcagctc cgccgccagg tcgcggtagg
     1381 ccgccgcgcc tttcgacgcg gggtcgtaat agttcaccgg cgcgccgaag ctgggggcct
     1441 ccgagaggcg cacattgcgc ggcaccaccg tcttatatac cttgttggga aaatactttt
     1501 tgacctccgc caccacctgc agcgtcaggt tgagccgccc gtcgtacatg gtgagcagca
     1561 cgccctcgat ctccaggtaa cggttgtaca tgtttttgac cgtccgcacc gtgctcatca
     1621 gctccgacag accctccagc gcaaaaaatt cgcattggat gggcacgagc accgtgtcgc
     1681 aagcgcaaag cccgttcaaa gtgagcaggt ccagcgatgg ggggcagtcg ataaagatga
     1741 aatcatactt ttgggccagc tgcgcgatgc cctcgcgcag ccgcgattcc ctgcggtcca
     1801 gcgccaccag ctcaataccc gcccccgaca gctggatgtt ggaggggatc aggtcgaccc
     1861 ggtatttggt gtgaagcacg cagctggagg ggtccccctc cccgatcagc agctgataga
     1921 cgctgttttt gacgcttcgc ttggggatac cgtagccgct ggtgctgttg ccctgagggt
     1981 caaggtccac cagcagcacc cgcttgcccg ccgcagccac cgccgaggcg aggttgaccg
     2041 ccgtcgtggt cttgccgacg ccgcctttct ggtttgcaat ggcaatgact ttactcactc
     2101 acatcaactc ctgtgctgtt tattatagca cagccaccga tacaggaaaa cccatttgag
     2161 ggaatgtttg cgttttctct ttttttgcca actgtttcac gtgaaacatt cttcttttag
     2221 gatgtaccgc cgggggcgct tctaatccac tgccaaataa tgcgcggaac aaaagcgttt
     2281 tctttgtcca aatcatctgt gtggtggtta gggctttttg ttcttatgcg gatcacagcc
     2341 tcttgactcc taggcgcgat gccgcccgcc gctttccctg cctcaggcgc aggagctgta
     2401 cacctgagct ttcacagaac aggagatctc taactttctc ccaaaatttt tccggcttgt
     2461 ctctctgatt catcccgacg cggccgccgg gggatgcctc ttttaagaga gcctttatgc
     2521 aaaaaactgc gagatctatt gatcccggtg aatctctatc caaaagatca ggcgctcttc
     2581 cgctagttcg gtctgtgccg cttcgccgca gggcgtttct ctctcccgcc ctattttctg
     2641 cgcccatagt gctgctgaaa cagagggctg gaaaagcctc ccacagctag tttcgatccc
     2701 ctttttctgg gcttgaaaaa atgtcagttg cgtcacacgg taaaaatgtt tcacgtgaaa
     2761 caaatttttc cagatttcac aaagaaaaaa cggcagagaa aatttctccg ccgccttttc
     2821 tgcaaacaat atatttagtg aagagttgcc ttatttgtgg ggatgcgcac gacatactgg
     2881 atgtactccc ctgtctcgct gcgctccatg ctggccggga tgccggagct cttcatcatg
     2941 tccacggccc ggttgaccgt gttgacaaag atgcgcacat cccgcaccat cccccgccgc
     3001 ttgggcttgg gacgcttttc ttttcccatc cgctccacca ggcgctccgt agcgctcacg
     3061 gtgagctgtt ctgagcggac cttttccacc gcttgcagct gctgggcgtt gctctccagc
     3121 cgcagcagcg cacgcacatg gcgctcggtg agctccgcct cttcggcggc ccggcgcacc
     3181 tccggcgcca ccgagagcag ccgaagcttg ttggccagtg cgctctgcga aaggcccagg
     3241 cgctttgcac cctcggtctg ggtgcagttc caaagggtga gcagctggcg cagcgcttcc
     3301 gcctcttcaa aagggcccag ctgctggcgc tgcaggtttt cttccagcga gagcaccgcc
     3361 gcgcggccat cctcataatc cgtcaggatg gccggcactt tctccatgcc gagcatctgc
     3421 gcggcccgca cccgccgttc gcccgctacc agggcgtagc ggtttttgcc cagctgctgc
     3481 agggtcaccg gctgcagcat cccgttttcg cggatactgg tcgccagcgc ctgcatctcc
     3541 ctcgggtcaa accggcggcg cgcctgccag ggcgacggtt cgatctggtc cagcgggacc
     3601 agcaccacct gccccgtcac ctgcaacctg gataaaaaca tggcttgtcc cgccctttcc
     3661 cccggccgcc ggcctcttaa aagcgccgtc cggcccaggt caaaaaaatc ctgtaaccgc
     3721 cccgaagctc tcgctttccg ggaacagtta caggatagca caaaagaatt gtaatttcca
     3781 tatatttcca tttacatgct tcgacacccc gccgtcattt cagcggggat tttgcgattt
     3841 tgccgccgtt tctggggtat accgtcgggg tttgcgatat ttttttaaca atgacaatgc
     3901 cccgctcgtc cccttccggc aaagaaaagc gccgcaggcc caccacctcg ccgcccagcc
     3961 tctcgatggc gggccgcgcc tcctgcagct cctcggcggc ggactcggct ttcatggcca
     4021 aaaaataccc ccccaccctt accaggggca aacaatactc cgccagcttg ttgagccgtg
     4081 ccaccgcgcg ggcggtggcg gtatcaaagc gctcccgcca ctgcttgcgg gccgcctcct
     4141 cggcgcgctc ttttaaaaac tccgcctcca cccccagctc gccgcaggcc cagcgcaaaa
     4201 agtccagccg tttgccggtg ggctccagca gcgtcagccg cagctcgggg cgatagagct
     4261 ttgccaccac accgggaaag cccgcgccgg tgcccacgtc caccaggcgc ccctgcgcct
     4321 ccggctgggc cgccagcagc agcgagtcga taaaatgctt gtcctcgatc ccctgcgggt
     4381 cggtgattgc ggtcaggttg accttttcat tgtactccac cagcagggcg gcgtaacggt
     4441 ccagcagggc cgcctgctct gccgtcaccg gcaccccgca gcgggcggcg ttggcagtaa
     4501 aacgctccat attgatcatc agtttctccc ctttccatcc cgggcgcggc gggcggccag
     4561 ctccactgag agcactgcga tatccgaggg attgaccccg gggatgcggg ccgcctgccc
     4621 caggtttttc ggccgcactc tcgagagctt ttcccgcgcc tccagccgga tgccctcaat
     4681 gggtccgtaa tccagctcgg gcgggatctt caccttttcc tgccgggcca gcgcctcgat
     4741 cttctgctgc tgcctgcgga tataaccctc gtatttgacc tcggtctcga tctggcccgc
     4801 cacgtgggcg cccacctttt cgcccggccc gatgacgccg gtgagcgccg cgtaaccgac
     4861 cgccgggcgg cgcagcagct ccaacgccgt ggccccggtc accagcggcg gctcacccag
     4921 cttttccagc agcgggttcg cctccgccgg gcgcagtacc cgggccgcga gccggtccag
     4981 ctcgcggcac tttgcggcct gatccgcctg aaaacgcgcc cagcgcgcgt cgtccaccag
     5041 ccccagctcc cgccccagcg gggtgagccg ctcgtcggca ttgtcctggc gcagggtgag
     5101 ccggtactcg ctgcggctgg tcatcatgcg ataggggtcg agcaccccct tggtcaccag
     5161 gtcgtcgatc agggtgccga tataactgga ctgccgctcc aggatcaccg gctcttttcc
     5221 ctgcacgctg cgggccgcgt tgacccccgc caccagcccc tgggccgcgg cctcctcata
     5281 tcccgaggtg ccgttgaact gcccggcgcc gaacaggccg gggatctgtt taaaggccag
     5341 cgaggcgtcc agctccagcg ggtcacagca atcgtactcg atggcatagg ccgggcgcat
     5401 gatctccagc tgctccagcc cgcggatggt gtgataaaag gcgttctgca cctcctcggg
     5461 cagcgagctg ctcatgccct gcagatacat ctcgtcggtg tcctcaccac agggctcgat
     5521 aaagatctga tgccgcggct tgtccttgaa gcgcatcacc ttatcctcga tgctggggca
     5581 gtagcggggc ccgatgccct cgatcttgcc gccgtacagc ggcgagcgcc cgatgttatc
     5641 gaggatgatg cggtgggtct gctcgttggt ataggtgata tggcagacca ccttgttgtg
     5701 cagctcgccg tccgtcatga agctgaaagg ctccaccggc tcgtctccct cctgcacctc
     5761 cagcgctgaa aagtcaatgc tgcggcggtt ggcccgggcc ggggtgccgg tcttgaagcg
     5821 gcgcaggctc acccccagcg cccgcaggct gtccgacagc cccagcgccg cgtgctggcc
     5881 atccggcccg ctctcatagc tggcctcccc cacatagatc ttccccgcca gataggtgcc
     5941 ggtggcaagg atcacggctt tcgcactata gccggcgcac aggcgggtct ccacgccggt
     6001 gacccggccg ccctcgctca gcagccgcac cacctccccc tgcttgagct ccaggttgtc
     6061 ggtgcgctcc aacaggcgtt tcatgtactc gtgatagcgc tggcggtcgc tttgcacccg
     6121 cagcgagtgc acggccgggc cct
//
LOCUS       DBMDYA010000070         6129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3268_length_6129_cov_1.806238, whole genome shotgun sequence.
ACCESSION   DBMDYA010000070 DBMDYA010000000
VERSION     DBMDYA010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_6129_cov_1.806238"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(91..861)
                     /locus_tag="ACZPFK_02760"
     CDS             complement(91..861)
                     /locus_tag="ACZPFK_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1405264.1"
                     /translation="MTPYQERADFILNQLEQLGEVRVKELSACLNCSEVTIRGDIRRL
                     EGEGLLKRTHGGAINKVRMLEVLCEPGEYTWNQQEKARIASCAYEYINNRDSIIIDDS
                     TTGGYLAKVIRQHPQKHIIVITNSLLAAAELSAAKHVELFIVSGHVVGAPPAALDNFT
                     IDAFHQFRVSKAFIGTNGISLRDGLFSLGAPQRDVKKAIISVSNEVYVLADHTKFDGG
                     NLFNICGMDEVTRVITDSKVSEEIIKETKERGIYMDVV"
     gene            complement(1006..1344)
                     /locus_tag="ACZPFK_02765"
     CDS             complement(1006..1344)
                     /locus_tag="ACZPFK_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HIR1405265.1"
                     /translation="MPFIHTMTNLPLSRQTEEKLKAAYGEAAELIGKSENWLMLRFED
                     GCRLWMAGGEEPAAMVQVDLYGKAAAAQYEAMTARLTAALEQELGLAPERVYVRYAET
                     PNWGWSGSNF"
     gene            complement(1417..1950)
                     /locus_tag="ACZPFK_02770"
     CDS             complement(1417..1950)
                     /locus_tag="ACZPFK_02770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1405266.1"
                     /translation="MDSFAELAARRQSCRAYDPSRQVEQEKLEACLQAARLAPSACNS
                     QPWHITLAQGGLAAQVGECLRGMGMNKFTADCPVFAVVTEDGYNPTAAAGARLKDQDY
                     RSVDIGIMAAHFTLQAADLGLGSCILGWFDEKKLQRLLGADKRVRLVLALGYPRPDDP
                     QRPKTRKPLAELVSRPE"
     gene            2263..3348
                     /gene="pyrB"
                     /locus_tag="ACZPFK_02775"
     CDS             2263..3348
                     /gene="pyrB"
                     /locus_tag="ACZPFK_02775"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1405267.1"
                     /translation="MRHFLTPLDLSLQELDSLLALADEIARDPAAYHGVCAGKKLATL
                     FYEPSTRTRLSFESAMLNLGGAVLGFSSASSSSVSKGETVADTIRILSCYADICAMRH
                     PVEGAPFRAALYSRIPVINAGDGGHQHPTQTLTDLVTIRRRLGRLDHLTVGFCGDLKY
                     GRTVHSLIAALSRYEGNRFVCISPEELRLPPFVKEEIAGAPSSALTETRALEEALPEL
                     NVLYMTRIQQERFTDRTVYERLKGCYVLTADKLAAARADMPVLHPLPRVDEIALEVDS
                     DPRAAYFEQAQNGVYARMALMMTLLGLAPVQLGEGAPLLPEGTERNILRCGDKRCITG
                     AEAGVDHLFVPTRDGSHRCFYCGRRGK"
     gene            complement(3470..4447)
                     /locus_tag="ACZPFK_02780"
     CDS             complement(3470..4447)
                     /locus_tag="ACZPFK_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012472.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1405268.1"
                     /translation="MKTQMRYGTVPYVDLPVSRVVFGTTQPMVRGEDVCELLDTVYAA
                     GINTFDSAASYGEAEASLGHWIRSRGNRKEIVLLTKGANPYPWRQRLTEYDILHDIEN
                     SFAKLGTDYIDVYILHRDDPAVPVGPIVELLNKLHQKGKIGAFGGSNWTLKRILEANQ
                     YAEEHHLIPFTVCSPSFSLAECIGDPWGGSVTISGEKNRDFRTWLKENEMPVFAYSSL
                     ARGFFSGRLKSSETERAKELLGPAAEEYGYPVNFERLRKAEQLAQEKRKTVSQIAFSW
                     LMHQELNLFGITAPGSKEHILSTVEALFLPLSKEELDWLSGSPAGLSTS"
     gene            complement(4504..>6129)
                     /locus_tag="ACZPFK_02785"
     CDS             complement(4504..>6129)
                     /locus_tag="ACZPFK_02785"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1405269.1"
                     /translation="AVAAWQIDNEFTAGDGLYCYCEECRQKFIVWLKKKYGSLDALNE
                     AWGTVFWSQTYTDWDQIIVPRRSAAHRKTGDREYNAHNPGLNLDYSRFQSDAIVDYSN
                     AQARILRRWSKMPITHNMVHEELDYYKFAEQIDICTFDNYPAVSMHLENRDPVVQTYN
                     PALQADIVRGAKNKNIWIMEEQSGPCGWNVLGSTPRPGQIREWSYQMMAHGAESIIYF
                     RWRACLFGTEQYWYGILDHDGVPRRRYEEIKAIGREMPKLAAYNENSRVDADILMIRS
                     FEQQWSHAFQQHSWNFDYRTYLFELYRGLEKGHYNLDVSNEAADFSNYKLVVAPAFNL
                     MDEALKDKFEKYVAEGGNLVITFRSGTKNMDNSMTERTLPGYFKELAGAEVEEFDSRF
                     RSSVGVKGDFGCGKASIWADILRPETAETVAVYTGDYFEGRPAITMNRYGKGKCYYVG
                     CDLDRDGTDALMEYIARQCGIAKAMAGLPNTVEVVRKKTSEGKTFYFLINPQSEAQNL
                     QLPFVARDLLTHKTLGSSFEISAYGAMAIEAEN"
BASE COUNT         1249 a         1913 c         1576 g         1391 t
ORIGIN      
        1 cgaaatctca acgtctttaa agtaaaaaac aaagaaccgc ccaggaagtt tttttcctgg
       61 gcggttcttt gttttctcaa caggggcagg tcatacgacg tccatataga tcccccgctc
      121 ctttgtctct tttataatct cctcgctcac cttgctgtca gtgatcactc tggtgacctc
      181 gtccatgcca cagatgttaa acagattacc gccgtcaaat tttgtgtggt ctgcaaggac
      241 atatacctca ttggagaccg agatgatcgc tttcttcaca tcccgctggg gggcccccag
      301 cgaaaacaat ccgtcccgca ggctgatgcc gttggtgccg ataaaggcct tagaaaccct
      361 gaactgatga aaggcgtcga tcgtgaaatt gtccagcgcc gcgggaggcg ccccgaccac
      421 atgcccgctg acgataaaaa gctccacatg ctttgccgca gacagctccg cggccgctaa
      481 aagcgaattg gtgattacaa taatatgctt ttgcgggtgc tgccgaatca cctttgccag
      541 ataaccgccg gtggtggaat cgtcaatgat gatggaatcc cgattgttga tatattcata
      601 tgcgcaggag gcgatgcgcg ccttttcctg ctggttccat gtgtattcac ccggctcaca
      661 caacacttcc aacatgcgca ccttattgat cgcgccccca tgggtccgtt tgagcaggcc
      721 ctccccctcc aaccgccgga tgtcgccccg tatcgtcacc tcggagcagt tcaggcaggc
      781 gctgagctct ttcacccgca cctctcccag ctgttcgagc tggttcaaaa taaaatctgc
      841 gcgttcctga tagggtgtca tatcaaaacc tcaaaatgga ttttcatacg aaatgctttt
      901 cttctggata attataacaa acgaaagaga tcaatacaac caaaacagag aaattatttc
      961 tctcccgcac ctaccgggcc gcatagcaca ccgcggggat cagggtcaaa aattgctgcc
     1021 gctccagccc caattcggcg tctcggcata tctcacataa acgcgctccg gggcgaggcc
     1081 gagctcttgt tccagcgccg cagtgaggcg ggcggtcatg gcctcatact gggcggcggc
     1141 ggccttgccg tacagatcga cctgtaccat ggcggcgggc tcctccccgc ccgccatcca
     1201 cagacggcag ccgtcctcga agcgcagcat cagccaattt tcactttttc cgatcagctc
     1261 cgcggcctcc ccataggcgg ctttcagctt ttcctccgtc tgccgcgaaa ggggcaggtt
     1321 ggtcatggta tggataaacg gcatagggat cttcctcctt tttgatcggg gcgcaggccc
     1381 cgtttggccc gtgcggccca aggcccgcac ggcgtttcac tccggccggg agaccagctc
     1441 ggccaacggc ttgcgcgttt tgggccgctg cgggtcgtcg gggcgcggat atcccagcgc
     1501 cagcaccagc cgcacccgct tgtcggcgcc cagcagacgc tgaagctttt tttcatcaaa
     1561 ccagcccagg atgcagctgc ccagccccag atccgcggcc tgcagcgtaa aatgcgccgc
     1621 catgatgccg atatccaccg atcggtaatc ctggtccttg agccttgcgc ccgcagcggc
     1681 ggtgggattg tatccgtcct cggtcaccac cgcaaaaacc gggcaatcgg cggtgaactt
     1741 gttcatcccc attccgcgca ggcactcgcc cacctgcgcc gccagcccgc cctgcgccag
     1801 cgtgatgtgc cagggctggc tgttgcaggc ggagggggcc agccgggccg cctgcaggca
     1861 ggcctccagt ttttcctgct ccacctgccg gctggggtca taggcgcggc agctctgccg
     1921 gcgcgcggcc aattccgcga aagaatccat ctctgtaagc acctctctct tttggctgca
     1981 gcggcgacca cgcccttttt ggggcctttt ctccaagggc ccggcgtgag gggccgggca
     2041 gcccggtggc gcggcgtgca gccgctcgct ttttacccag tatatcatat ttttgaagcg
     2101 ccaaaacgcc aaaaacgccg aaaatccgct gctgcgttta caatcctttc tgcggcaggt
     2161 ataattttag acagtttttt accgactttc tttctgcggc tccgcgtccg ccctggccgg
     2221 gcgcgcgctc cctttcactc caccacacag gaggtctttt tcatgcgtca ctttctcacc
     2281 ccgctcgacc tttcgctgca agagctggac agcctgctcg ccctcgcgga cgagatcgcc
     2341 cgcgacccgg ccgcctatca cggcgtctgt gcaggcaaaa agctcgccac tcttttttac
     2401 gaacccagca cccgcacccg gctctccttt gaatccgcca tgctgaacct gggcggcgca
     2461 gtgctgggtt tttcctccgc cagctccagt tcggtctcca agggggagac agtggccgac
     2521 accatccgca tcctctcctg ctacgcggat atctgcgcca tgcgccaccc ggtggagggc
     2581 gcgccgttcc gagccgcgct ctactcccgc atcccggtca tcaacgccgg ggacggcggc
     2641 catcagcacc ccactcagac cctcaccgac ctggtgacca tccgccgccg gctggggcgg
     2701 ctggaccacc tgaccgtggg cttttgcggc gacttgaagt acggccgcac ggtgcactca
     2761 ctgatcgcgg cgctctcccg ctatgagggc aaccgctttg tctgcatcag cccggaggag
     2821 ctgcgcctgc ccccctttgt caaagaggag atcgcaggcg cgcccagcag cgcgctgacg
     2881 gagacccgcg cgctggagga ggccctgccg gagctcaacg tgctctatat gacccgcatc
     2941 cagcaggagc gcttcaccga cagaaccgtc tatgagcggc tcaagggctg ctatgtcctc
     3001 actgcggaca agctggccgc ggcccgggcc gacatgccgg tgctgcaccc gctgccccgg
     3061 gtggacgaga tcgcgctgga ggtggacagc gacccccggg ccgcctattt cgagcaggcg
     3121 caaaacggcg tgtacgcccg tatggccctg atgatgaccc tgctgggcct ggcccccgtc
     3181 cagctgggcg agggcgcgcc gctgctgccg gaggggaccg agaggaacat cctgcgctgc
     3241 ggcgacaagc gctgcatcac cggcgccgaa gccggcgtcg atcacctttt cgtccccacc
     3301 cgggacggca gccaccgctg cttttactgc gggcggcgag gcaagtaaaa aatatgagcc
     3361 gatctgctgc gcccccatgg ctcactcaga atattggata caaaagcaaa agagccgcca
     3421 ggaagcctgg gcagctcttt tgcttttctc taaagaaaga ccagaggttc taagaagttg
     3481 agagacctgc tggacttcca ctgagccagt ccagttcttc tttgctcagc ggcaaaaaaa
     3541 gcgcttctac tgtagaaagg atatgctctt tcgaccccgg tgcagtgatt ccaaacaggt
     3601 tcagttcttg atgcatgagc caagaaaaag caatttgaga gaccgttttc cttttctctt
     3661 gcgccagttg ctctgccttg cgaagccgct caaaattgac cggataccca tattcctccg
     3721 cggcagggcc cagcagctct tttgcgcgct ctgtttcaga acttttcaat cgccccgaaa
     3781 agaagccgcg cgccaggctg gaatacgcaa agacaggcat ctcgttctct ttcaaccaag
     3841 tccgaaaatc cctatttttt tctccagaga tagtgacgct gccgccccag gggtctccga
     3901 tacattccgc caggctgaaa ctggggctac ataccgtaaa aggaatgaga tgatgctcct
     3961 ctgcatactg atttgcctct aaaatccgct ttagtgtcca gttagagccc ccaaaagccc
     4021 caattttccc tttctgatgg agcttgttga gcagctctac gattgggccc accggtacgg
     4081 cggggtcatc ccgatgaagg atgtagacat cgatatagtc tgttcccaac ttggcaaaag
     4141 agttttcgat gtcgtgcaga atgtcatact cggtgagccg ttgccgccag ggataaggat
     4201 ttgccccctt ggtcaacagc acgatctctt ttcggttgcc tcgagagcgg atccagtgcc
     4261 ccaaggacgc ttcagcctcc ccatagcttg ccgcagaatc aaaagtattg attccagcgg
     4321 catacactgt atccagtaat tcgcacacat cttcccctct caccatgggc tgggtcgttc
     4381 caaaaacgac ccttgaaacc ggtaaatcca catagggcac ggttccatag cgcatttggg
     4441 ttttcatttc acaccttctc tttcattcaa cggctccgcc tcagagtctt tttggcattc
     4501 gcttcagttc tccgcctcta tggccatagc cccataggca gagatttcaa aagaactgcc
     4561 cagtgtcttg tgtgtgagga gatctctcgc cacaaaggga agctgcagat tctgcgcttc
     4621 actttgcgga ttgatcagga aatagaatgt cttcccctca gaggtctttt tgcgcaccac
     4681 ttctacggta ttgggtaatc ccgccatcgc ttttgcaata ccgcactgac gggcgatgta
     4741 ctccatcagt gcatccgtgc catcccggtc gaggtcgcag cccacataat agcattttcc
     4801 tttcccatag cgattcatcg taatggccgg acgcccctca aaatagtccc cggtgtatac
     4861 cgcaaccgtc tcggcggtct ccggccgcag gatatccgcc caaatgctgg ctttgccgca
     4921 gccaaaatct ccttttaccc caacggaaga acggaagcgg gaatcaaatt cttctacctc
     4981 tgcgcccgcc aactctttga agtagcccgg cagggttcgc tcggtcatcg agttgtccat
     5041 gttcttggtg ccggatcgga aagtgattac caggtttccg ccttccgcta cgtatttctc
     5101 aaatttatct ttcagcgcct catccatcag attgaatgcc ggagccacca ccagtttgta
     5161 atttgaaaaa tccgcggctt cattggagac gtccagattg taatgtccct tttccaggcc
     5221 ccgataaagt tcaaaaagat aagtgcgata atcaaaattc cagctatgct gttgaaatgc
     5281 gtggctccat tgctgctcga acgagcggat catcaaaata tccgcatcca ccctggagtt
     5341 ctcgttatag gcggccagct tcggcatttc tcttccgatc gctttgatct cctcatagcg
     5401 gcggcggggc acaccgtcat ggtccagaat accgtaccag tactgctcag tgccgaacag
     5461 gcaggcgcgc cagcggaagt aaatgatcga ctctgccccg tgcgccatca tctgatagct
     5521 ccattcccgg atctggccgg ggcgcggggt actccccaat acgttccacc cgcagggacc
     5581 actctgctct tccataatcc aaatattttt atttttggcg ccgcgtacaa tatccgcctg
     5641 tagtgcagga ttgtacgtct gtaccacggg atcccgattt tcaagatgca tactgacggc
     5701 aggatagtta tcaaaagtac agatatcaat ctgttcggca aatttgtagt aatccagctc
     5761 ttcatgcacc atgttgtggg tgatgggcat tttgctccac cggcgcagga tacgtgcctg
     5821 agcattgctg tagtccacga tggcatcgga ttgaaagcgg gaataatcca gatttaatcc
     5881 ggggttgtgc gcattatatt ctctatcgcc ggtcttacgg tgtgcggcgc ttctgcgcgg
     5941 cacaatgatc tggtcccagt cggtataggt ctggctccag aacacagtcc cccaggcctc
     6001 gttcaacgcg tccaggctgc cgtatttttt cttcagccag acgataaact tctgcctgca
     6061 ctcctcgcaa tagcagtaca gtccgtcgcc tgcagtaaac tcgttgtcga tctgccaggc
     6121 ggccaccgc
//
LOCUS       DBMDYA010000071         6108 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3280_length_6108_cov_1.847678, whole genome shotgun sequence.
ACCESSION   DBMDYA010000071 DBMDYA010000000
VERSION     DBMDYA010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_6108_cov_1.847678"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="ACZPFK_02790"
     CDS             <1..1216
                     /locus_tag="ACZPFK_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405270.1"
                     /translation="AGLFIDREKAVRMAEEISLKYNLAINPREKVRDLSVGSKQKVEI
                     LKALLRGAEILILDEPTAVLTPQETEELFKELQAFKEAGHTIIFISHKLREIKELCDR
                     ITIIRAGKTMGTFRVADVSEQDISRLMVGRDVILSVDKEPAVPREVILSVRGLNYTDS
                     AGVQVLKDVNLNLRRGQILGVAGVEGNGQSELIQAVVGLAHFRSGEIIFDGDAIRGKS
                     IRQIREMGMAYIPQDRMTMGIAPNISIQDNLISDRYYRGDYSGRAFLKAQHIKRETEK
                     LVDEFQVVCTDRLQSVGSLSGGNIQKVVVAREFSSTPKAVIAEQPTRGVDVGATEFIR
                     RRIVSLRDAGAGVLLVSADLNEVLELSDSIIVMFGGEVAAYFPDSSQVTEEMLGLYML
                     GIQKMNPSEILF"
     gene            1227..2300
                     /locus_tag="ACZPFK_02795"
     CDS             1227..2300
                     /locus_tag="ACZPFK_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405271.1"
                     /translation="MGTNIRKVNLIVDAVKLLATVLISLACVTVLILIVSDEPANALR
                     AFFLGPFTSPRRMSQVLQLATPILFTGLAVTVMLRAGLFNMGSEGALYFGGICATFVA
                     VNMQLPPVFHTVAALLLAAVGGGLVGVVPAVMRAKFGINELVVSIMMNYVMISLSMFV
                     LNYYLKDASVSARISKSFPDYVKIPKMFGTAHFGFVLAVATAVFVYLLLYKTKLGYEI
                     RISGDNGIFASYSGVKTLKVIVLSQVIGCAVAGLGGGAETLGSYNAFSWDGATGYGFD
                     GMVVSIIARRNPLLAVVAALFLGYLRAGADIMNRTSDVALEVIQIVQAIMIVLVAAEA
                     FLDGWRHKLTIKESRTAKEVHAR"
     gene            2297..3226
                     /locus_tag="ACZPFK_02800"
     CDS             2297..3226
                     /locus_tag="ACZPFK_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405272.1"
                     /translation="MNLILETLFSPNTITATIRVLTLLIYGTMAAVINVTSGINNIGI
                     EGILLSSALVAVVGSWATQSWLVGTVCAVVWGMAISSLFGYLVMKLKTNNILCGLAIN
                     MFASGATVFLLSALTGQKGSSISLASKSAPKLSLPLLREIPYVGILFEDYSLFTYLAV
                     FVVLFVIIMIRKTPIGMRIRAVGLNPNAASSVGIRVNRVRYTAFLMSGAIAGLGGAYL
                     TMGYFSSFTRDMTAGIGFMCIAACNMGGATPLGGLLSAILFAFANVFSNVMQSLQMSV
                     DLINGFPYMITVVALFIIGYVNYRKARKAAAKK"
     gene            complement(3634..4758)
                     /locus_tag="ACZPFK_02805"
     CDS             complement(3634..4758)
                     /locus_tag="ACZPFK_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1405273.1"
                     /translation="MNKHEKIILEKLTEDPMLSQTELARMLNITRSSVSVYISYLMQK
                     GYIRGRGYVIEDQKNVCVIGSSGVDYQTVMAENQIFTPLALDDYDLRVSYGGICKNIA
                     ESLTRFGLRTSCISAVGSDLLGQSLIEECKSAGVDTDNFMIVPSERTSTYLEIRTLDF
                     NKIILASANMKLQRLITPEFLSSKQYKLRHAQLIVVEDSLSSETLKYVSSTYSPTLFV
                     CTKPTRIKRYKDFLNQFNGMVTSLENAAAILGRETPDASDEKSVFRISQQLHAQLAGP
                     LLVCYGDSHFCYTDDEASVICVFSDPTPRSALFSHYRDTVATGFIRSFINDDDPTTLL
                     KAVSACREIAAQSTAFCSQGLCSELVEKMVCKKKFHFSYQ"
     gene            4957..5670
                     /locus_tag="ACZPFK_02810"
     CDS             4957..5670
                     /locus_tag="ACZPFK_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1405274.1"
                     /translation="MGKFLNPERNRKLPLKVGLYFTGMAVSCCGTAIFTLNGLGSDAM
                     NTLFAAVAARAGILPGSIYTVFNTTMLLAGFMFARRYMGVGSVLMILVQGFFIDAWMQ
                     GLLSMPWLFQGAAWKTVMAVVGYLCRTFGCALSTSMCLGTAGFEACLFTLADKIRVEY
                     KYLKMLSEVFYFVAALFLGGVYGVMTIIEVLFYGHGISFFMVRLNRTVWRRWGIEDKR
                     NDLSRNSRHKVSTGGPTNG"
     gene            5766..>6108
                     /locus_tag="ACZPFK_02815"
     CDS             5766..>6108
                     /locus_tag="ACZPFK_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405275.1"
                     /translation="MKKLLAILLALVMAASLVACGGSGGGDKPGSGGASSGAAQQGGS
                     ATTGKDTHSIIYLCMNLGDLSFNDLGWEGSQKAAEKYGWSADVIELGKDTSTYENAFV
                     DALDSGKYDLVV"
BASE COUNT         1397 a         1457 c         1755 g         1499 t
ORIGIN      
        1 agcaggcctt tttatcgacc gggaaaaagc agtgcgtatg gcggaagaga tttctttaaa
       61 atacaatctg gcgatcaacc cgcgtgaaaa agtgcgggat ctttcggtgg gaagcaaaca
      121 gaaagtggag attttaaaag cccttttgcg gggagcggag attttaattt tggacgagcc
      181 caccgcagtg ctgaccccac aggagacgga ggaattattc aaagagcttc aggcctttaa
      241 agaggcgggc cacacgatca tttttatctc acacaagctg cgggagatca aagagctctg
      301 tgatcgcatc acaatcatcc gggcgggaaa aacgatgggg acatttcggg tggcggatgt
      361 atccgagcag gatatttcaa ggctgatggt gggccgggac gtcatcctca gcgtggacaa
      421 ggagccggcc gtcccccgcg aggtcatcct ttccgtgagg ggtctgaatt atacggactc
      481 cgcaggcgta caggtattaa aagatgtcaa tttaaatctg cgccgcgggc aaatattggg
      541 cgtcgcaggg gtggagggga atggccaaag cgagctgatc caggcggttg tggggctggc
      601 ccatttccgc tccggcgaga tcatttttga cggcgacgcc attcggggga aaagcatccg
      661 tcagattcgt gaaatgggga tggcatatat cccccaggac aggatgacca tggggattgc
      721 ccccaatatt tcgattcagg acaacttgat ctctgaccgt tattataggg gcgattacag
      781 cggaagagcc tttttaaaag cgcagcatat aaaaagagaa accgagaaac tggtagacga
      841 gtttcaagtg gtatgcacag accgcttgca gtctgtgggc tccctctcag ggggaaatat
      901 ccaaaaggtc gtcgtggctc gtgagttttc ttcaacgccc aaggcggtga ttgcggagca
      961 accgacccga ggcgtagacg tgggggcgac cgagtttata cgccgccgca tcgtctcgct
     1021 gcgagatgcg ggcgcggggg tcttgctcgt ctcagcggat ttgaacgaag tcttggaact
     1081 gagcgacagc attatcgtga tgtttggagg cgaggtcgcg gcctattttc ctgatagctc
     1141 gcaggtgacg gaggaaatgc tggggctata tatgctcggc atccaaaaga tgaacccctc
     1201 tgaaattctt ttttaggcgg tgtgaaatgg ggacaaatat tcgaaaagta aatctgatcg
     1261 tagacgctgt aaaactttta gccactgttc ttatttccct tgcctgcgtc acggtactta
     1321 tcctgatcgt cagcgatgag ccggcaaacg cgctgcgggc ttttttcctg gggcccttta
     1381 cctccccgcg gcgcatgagc caggtgttgc aactggcgac gccgatcttg ttcaccgggc
     1441 tcgcggtcac agtgatgcta cgggccggac tttttaatat gggctctgag ggcgcgctgt
     1501 attttggcgg gatctgtgca acttttgtag cggtaaacat gcaactccct ccagtttttc
     1561 acacggtcgc ggcgcttttg ttggctgcgg tgggcggcgg gttggtgggt gtagttcctg
     1621 cggtcatgag ggcaaagttc gggatcaacg aactggtggt gtccatcatg atgaactatg
     1681 tgatgatcag cctgagcatg tttgttttga actactattt aaaggatgcc tcggtctccg
     1741 cgcgaatatc gaaaagtttt ccagactatg tgaaaatacc caaaatgttt ggcaccgcgc
     1801 attttggctt cgttttggca gtggcgaccg ctgtttttgt ctatttgctg ctgtataaaa
     1861 caaagctggg atacgaaatc cgcatttccg gcgacaatgg tatctttgct tcttattccg
     1921 gagtaaagac cttgaaagtg attgtgctct cgcaggtgat cgggtgcgcg gtcgccggcc
     1981 ttggcggcgg tgcagaaaca cttgggtcgt ataatgcttt cagttgggat ggggcgactg
     2041 gatatggctt tgacggcatg gtggtctcaa ttatcgcgcg gcgaaatccg cttctggcgg
     2101 tcgtggccgc cctgttttta ggatacctgc gcgccggagc ggatattatg aaccgcacct
     2161 cagatgtggc gctggaagtg atacagatcg tacaagcgat catgatcgtc ctggtggcgg
     2221 cagaagcttt tctggatggg tggaggcaca aactgacgat caaagaatcc cgcacggcaa
     2281 aggaggtgca cgcgcgatga atctgatcct tgaaaccctg ttttcaccca acacgatcac
     2341 ggcgacgatc cgcgttttaa cccttcttat ctacggtacc atggccgcgg tgatcaatgt
     2401 gaccagcggt atcaataata ttgggataga gggaattctc ctctccagcg cgcttgtcgc
     2461 agtcgtgggc agttgggcga ctcaaagctg gctcgtggga acagtttgcg ccgtcgtttg
     2521 ggggatggca atcagctcgt tattcggcta tctggtcatg aagctgaaaa caaataacat
     2581 tttgtgtggc ttggccatca atatgtttgc gtcgggcgcc actgtctttt tgttatcggc
     2641 gctgactggg caaaaagggt cttccatctc tttggcaagc aaaagcgccc ccaaactttc
     2701 tttgccgctg ctgagagaaa ttccttatgt ggggatcttg tttgaggact acagtctttt
     2761 tacctatctg gcggtttttg tagtcctttt tgtcattatc atgatccgca aaacaccgat
     2821 cggcatgcgc atacgagccg taggcctcaa cccgaacgcg gcgagttctg tggggattcg
     2881 ggtaaaccgg gtgcgctata ccgctttttt gatgtcgggg gccatcgcgg gcctgggcgg
     2941 cgcctacctc acgatggggt atttttcctc ctttacacgg gacatgactg ccggcattgg
     3001 ctttatgtgt attgccgcgt gcaacatggg cggagcaacc cctcttgggg ggcttttatc
     3061 ggcaatcctt tttgcctttg ccaacgtttt ttccaacgtg atgcagtcgc ttcagatgtc
     3121 tgtagacttg atcaatggtt tcccctatat gatcacggtt gtcgctctat ttatcatcgg
     3181 ctatgtcaat tacagaaaag cgcgcaaggc ggcggccaaa aagtgaaaac agtatttttc
     3241 ccttgccgac agacagcggg tccccgagga gccttgttgc agcctcgctt ttcagagggt
     3301 aataaaaagt agaaactgtt ttgtagaaag ttggtgggac gccccccggc agaggaagac
     3361 ggcgctgtgc agagcagccc ggtgagaaga aagacgactg tcgtataaaa acatatacgg
     3421 agacaagacg aaccataccg gagaaaaaat ttaaggccaa agaaaaagaa cgcccactgg
     3481 aggcggagca agcaatcgcg taccgcccgg gcctgaacaa agtgaaagcc agcgcttttt
     3541 aagcgctggc ttttttcggc tttacgagcc gtcctccgtg accactcttt ttggccgtga
     3601 ttctaatatt gtaaaaaaga gcatccgacc aactcactga taggaaaagt ggaacttttt
     3661 cttgcagacc attttttcca ccagttcgct gcacaacccc tggctgcaga aagcagtgga
     3721 ttgtgcggca atctctcgac aagccgacac cgccttgagc agtgtggtcg gatcgtcgtc
     3781 gttgatgaaa gagcggataa aaccggtggc caccgtgtca cggtaatggg aaaaaagagc
     3841 cgagcggggc gtcggatcgg aaaaaacaca gatgacgctg gcctcgtcgt cggtataaca
     3901 aaaatggctg tccccgtagc agaccaacag ggggccggca agctgcgcgt gcagctgctg
     3961 agagatccga aacacgcttt tttcatccga tgcgtccggc gtctcccgcc ccaggatggc
     4021 ggctgcattt tccaggctgg tcaccatgcc gttgaactgg ttgagaaaat ctttatagcg
     4081 tttgatgcgg gtgggcttgg tgcagacaaa cagcgtgggc gaataggtgg aggaaacata
     4141 tttcagggtt tcactggaca ggctgtcctc gaccacaatg agctgggcgt gccgcagctt
     4201 gtactgcttg ctgctcaaaa actcaggggt gatcaggcgc tgcagcttca tgtttgccga
     4261 ggccaggatg atcttgttga aatccagggt gcggatctcc aggtaggtgg aggttcgctc
     4321 cgagggcaca atcataaaat tgtcggtgtc caccccggca cttttacatt cctcgatcag
     4381 agactgcccc agcaggtcgg agccgacggc ggagatacag gaggtgcgca gcccaaagcg
     4441 ggtcaaagac tccgcgatat ttttacagat accgccgtag ctgacccgca ggtcatagtc
     4501 atccagcgcc agaggcgtaa aaatctgatt ttccgccatc acggtttgat agtccacgcc
     4561 ggaggagccg atcacacaga cattcttttg atcctctatc acgtacccgc gcccgcggat
     4621 ataacccttt tgcatcagat aagagatata aacggatacc gaggaccgcg tgatgttcag
     4681 catccgtgcc aattcagttt gggagagcat ggggtcttcg gtcagttttt ccaagatgat
     4741 cttttcatgc ttgttcactg tgatcgcacc tttgtactgt ccattttcta acatttattc
     4801 tatcgacatt cagtcaaaaa gtcaataaat ataaaaatat cacaattatt tagattaaat
     4861 gttataactt ttgtgtattt actcttaaag aaatatagca tatactattt ttaaatcaaa
     4921 aagctacaac gctccgcaaa tttgaaggag aaccgcatgg gaaagttctt gaatccggaa
     4981 cgcaatagaa agctcccgct caaggtaggc ctctatttca cgggcatggc ggtgagctgc
     5041 tgcggcacgg cgatctttac cctgaatggg ctgggcagcg acgcgatgaa cacgctgttc
     5101 gccgccgttg ccgcaagggc gggtattttg ccggggtcga tctacacggt attcaacacc
     5161 accatgttgc tggcgggttt tatgtttgcc aggcgctata tgggcgtcgg gtcggtgctg
     5221 atgatcctgg tgcagggctt ttttatcgac gcctggatgc aggggctcct gtctatgccc
     5281 tggctgttcc agggagcggc gtggaagact gtaatggcgg tagtggggta tctgtgccgg
     5341 acgttcggct gtgcgctctc cacctccatg tgtctgggca cggcggggtt tgaagcttgc
     5401 ctcttcacct tggcggacaa aattcgggtg gagtacaaat atttaaagat gctcagtgaa
     5461 gttttttact ttgtggcggc cctgttcctg gggggcgttt acggtgtgat gacgatcata
     5521 gaagtcctgt tttacggcca cggcatcagc ttttttatgg tgcggctgaa ccggaccgtg
     5581 tggagacggt gggggataga ggacaaacgc aacgacctct cgcgcaacag ccggcataag
     5641 gtgtcgaccg gcggccccac aaacggttag agtccttatt tccgctttca agcgctttga
     5701 agcggatggg agaatgaaag gagcgttcat tttatttaca cctggtaaaa gaaaggaaga
     5761 agatcatgaa aaagttactt gctatcctac tggcactcgt tatggcggct tcacttgttg
     5821 cgtgcggcgg ttccggcggc ggggacaagc ccggttccgg cggcgcctcc tccggcgcgg
     5881 cgcagcaggg cggctccgca acgaccggca aggacaccca cagcatcatc tatctgtgca
     5941 tgaacctggg agatctgtct ttcaatgatc tgggctggga gggctctcag aaagcggcgg
     6001 aaaaatacgg ctggagcgcg gacgtcatcg aactgggcaa ggacacctcc acctatgaaa
     6061 acgcttttgt ggacgccttg gacagcggca aatatgacct ggtggtca
//
LOCUS       DBMDYA010000072         6105 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3283_length_6105_cov_1.597268, whole genome shotgun sequence.
ACCESSION   DBMDYA010000072 DBMDYA010000000
VERSION     DBMDYA010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_6105_cov_1.597268"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="ACZPFK_02820"
     CDS             <1..303
                     /locus_tag="ACZPFK_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405276.1"
                     /translation="IKNSRCAAIFRPCVSVAAAAARNGAVKIDCFFGPVARLRAAVLE
                     IRLFFGCSASPMAGSIKTGRFLLHCNKKYAATSLSGLPRLEEGRGSPDKPPFIAER"
     gene            523..1650
                     /locus_tag="ACZPFK_02825"
     CDS             523..1650
                     /locus_tag="ACZPFK_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1405277.1"
                     /translation="MKAVKEYIRSTDKLFILLCMACSALSVVALTSIAHDSASAQNSL
                     FSNYRLPIVQAGAAALGLLCAVVISTFDYHALAASWPFHVAVSWGLVLLTFVIGYAPP
                     GTTNKSWIELPMGLSLQPTELAKISFILSFALHLDNAKDRMNEPPTMMALLAHLGAPL
                     LLIHFQGDDGTALIFLLIGVSMLFVAGLSRRFIYAGCGLAAVAVPLLWFGIMEDYQKK
                     RIYALFDPDSYTDVMWQQNQARISIGAGQITGRGFFGVEHHPVPLAQNDFIFSYISES
                     LGIIGSLLVLGLLFGLAAKLVGTAFRSQDRLGALICVGISAVIVWQTIINVGMNLSLL
                     PVIGITLPFFTAGGTSVLMLYLCMGLALSVYTHNKRNLFDG"
     gene            1797..2825
                     /locus_tag="ACZPFK_02830"
     CDS             1797..2825
                     /locus_tag="ACZPFK_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1405278.1"
                     /translation="MKPKMSRRKKIVLIALGAVLGLLALLAALAVLFINSKMSLLDRS
                     ASYGAGSLSPDEEELSFDHLDLDYIDVMEGGGSIEPPQGEVNTHPGITNILLVGSDER
                     GEDISSSARADSIMLLSVDSKANTWKLVSFERGVGVSIPGHGDDWLTHTFAYGGPELL
                     MQTLREYYKVDVSRYVRINFSIFETGVTDIGGVEVELTQAEADYMNGIAGEKKWSAGP
                     ARLDGPTALVYARMRHLDSDWQRVERQRKVLQAAADQIATLSPAKINLIADKLLPMIE
                     TNLTNGELWQLAFKMPALLGSDAAQLTVPDEDACWGIKLSSGQSLIGCDFAAEAERLD
                     RFLTGAAG"
     gene            3041..4144
                     /locus_tag="ACZPFK_02835"
     CDS             3041..4144
                     /locus_tag="ACZPFK_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR1405279.1"
                     /translation="MKRNLKRLGIALGVLLGAAVLAVGGYVLYMVLQYDRIPDHQPLT
                     VENPQTAQLCRGQAYTAATYNIGFGAYDPDFSFFMDTGTMKDGTPVQGEHGRAQSEQI
                     VLKDTQGAIDTLQGLGPDFCLLQEVDTDATRSFHIDQSQMVRQAFPQYGAVFASNFHS
                     AYLAYPLHEMHGSVQAGLLTLSRYAVAQAERRSYPVDESFPTKFFDLDRCFSVQRLSV
                     AGGGELVLINSHMSAYDEGGTVRAAQLELLGGVLREEREKGNYVIVGGDFNHALCGTV
                     QAFASGQQIPAWVFEFSDSDLPEGFHVVEAENLTEVPTCRSSDMPYTPGVNYTAVLDG
                     FIVSDNLTATARNVDTGFAYSDHNPVLLTFVLG"
     gene            complement(4227..4448)
                     /locus_tag="ACZPFK_02840"
     CDS             complement(4227..4448)
                     /locus_tag="ACZPFK_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR1405280.1"
                     /translation="MIKKICLALLIIGGLNWGLVGLFRFDCVGWLLGGTASIFSRIVF
                     TIVGLAALVAIPMLFERERPEQPGRPDGE"
     gene            4658..4858
                     /locus_tag="ACZPFK_02845"
     CDS             4658..4858
                     /locus_tag="ACZPFK_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1405281.1"
                     /translation="MTITKETIIGDILDAAPETAPLFLEIGMHCLGCPSSRGETIAQA
                     CYVHGTDADALVEKLNAAVAGK"
     gene            4858..5550
                     /locus_tag="ACZPFK_02850"
     CDS             4858..5550
                     /locus_tag="ACZPFK_02850"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF024255.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIR1405282.1"
                     /translation="MQPLRPGARLAAAAGLVRPGSRAADIGCDHGRLAAFLVESGRCP
                     FVVAADLRPGPLEKARRLAAARGLEGRVDCRLGDGLSVLRPGEVEDIVVAGMGGETIA
                     EILAAAPWVRRPGCNLVLLPASGQPQLRRWLCENGFALLREVPVTEGRYCYTVLQAAY
                     TGERVSPGPLFCWLGLLRGQRGPAAKAYFAKLLRQLEKELCGRERAGETGLEGLRRLT
                     EEIGKELAECRE"
     gene            5538..>6105
                     /locus_tag="ACZPFK_02855"
     CDS             5538..>6105
                     /locus_tag="ACZPFK_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1405283.1"
                     /translation="MPRVEEIRAFLDELAPQALAEDWDNVGALVDCGSNVTSVMMALD
                     ITDEVVTEAEMAGCQLIVSHHPVIFHPLRSVSRSDVAFRMVKKSISGICMHTNMDAAD
                     GGVNDILAIIFGLQEVKKLDGMGRVGRLRAPTGAAQLAALCREKFGAAVKYVDAGRPV
                     STLAVVGGSGGDFIPAALAAGADSLLTGE"
BASE COUNT         1078 a         1863 c         2049 g         1115 t
ORIGIN      
        1 ataaaaaata gccgctgtgc ggctattttt aggccgtgcg tttcggttgc cgcggcagcg
       61 gcgagaaacg gggccgttaa gatagattgt ttttttggtc ctgtggcccg cctccgggcc
      121 gcggtcctgg aaatcaggct gttttttggc tgttccgcca gtcctatggc cgggagcatt
      181 aaaacaggtc gttttttatt gcattgcaat aaaaaatacg ctgcgaccag cttatccggg
      241 ctgccccggc tcgaagaggg gcggggcagc ccggataagc cgccttttat cgcagagcgg
      301 taatgggccg caagacaaga caccggggca caagggagct gttgacaaac caagagccgc
      361 agaaaacgca ggcgattttt ggggcagatg aaacaaaaaa cgcaggtaag cttttgtggc
      421 ttaacgggta tttttgtgaa atatgccgca acaaagcgcc cctgttttca aggtgcgact
      481 ttgtcaacag ccccacaaga ccccaaagtg aggagacctg acttgaaagc cgtcaaagaa
      541 tacatccgtt ccaccgacaa gctgttcatc ctgctctgca tggcctgcag cgccctgtcg
      601 gtggtggcgc tcacctcgat cgcacacgac agcgccagcg cccagaacag cctgttcagc
      661 aactaccgcc tgcccatcgt gcaggcgggc gcggcggcgc tggggctgct gtgcgccgtc
      721 gtcatctcca cctttgacta ccacgcgctg gccgccagct ggccctttca cgtggcggtg
      781 tcctgggggc tggtgctgct cacctttgtg atcggctatg cgccccccgg caccaccaac
      841 aaatcctgga tcgagctgcc catgggtctg tccctgcagc ccaccgagtt ggcgaagatc
      901 agctttatcc tctcgtttgc gctgcacctg gacaacgcca aggacaggat gaacgagccc
      961 cccaccatga tggctctgct ggcgcatctg ggcgcgccgc tgctgctgat ccactttcag
     1021 ggcgacgacg gcacggcgct gatctttttg ctcatcggcg tgtcgatgct ctttgtggcc
     1081 ggcctctcgc gccggttcat ctatgcgggc tgcggcctcg ctgcggtcgc ggtgccgctg
     1141 ctctggttcg gcatcatgga ggactaccag aaaaagcgca tctacgcgct gttcgacccc
     1201 gacagctaca ccgacgtcat gtggcagcaa aaccaggcgc gcatctccat cggcgcgggg
     1261 cagatcaccg ggcgcggctt ttttggggtg gaacaccacc cggtgccgct ggcccagaac
     1321 gacttcattt tttcttatat ctccgagtcg ctgggcatca tcggcagcct gctggtgctg
     1381 gggctgctgt tcggcctggc ggccaagctg gtgggcacgg cgttccgcag ccaggacagg
     1441 ctgggggcgc tgatctgtgt gggcatctcg gcggtcatcg tctggcagac catcatcaac
     1501 gtgggcatga acctgtcgct gctgccggtc atcggcatca cactgccctt tttcaccgcc
     1561 ggcggcacct cggtgctgat gctgtatctc tgtatggggc tggccctctc ggtctacacc
     1621 cacaacaagc ggaatttatt cgatggctga agttcccttt tccccgcggc agtgccggtt
     1681 ttggctgcgc cgcggggctt cttttttgcc ctccgtcgtt ttcactgaat tttaacagaa
     1741 agaggctaca ataaaacacc ttgagaccgc cggagaaaaa aagggaggcg agcacggtga
     1801 aaccgaagat gtcaagacgg aagaagattg tcctgatcgc cctgggcgcg gttctgggcc
     1861 tgttggcgct gctggcagcc ctggcggtgc tctttatcaa cagcaagatg agcctgctcg
     1921 accgctcggc cagctacggg gcggggtcgc tctccccgga cgaggaggag ctctcctttg
     1981 accatctgga cctggactat atcgacgtga tggagggcgg cggcagcatc gagccgccgc
     2041 agggggaggt caatacccac cccggcatca ccaatatcct gctggtgggc tcggacgagc
     2101 ggggagagga tatctcctcc tccgcccggg cggacagcat catgctgctc tcggtggaca
     2161 gcaaagccaa cacctggaag ctggtttcct ttgagcgcgg ggtgggggtc tcgatccccg
     2221 gccacgggga cgactggctg acccatacct ttgcctacgg gggccccgag ctgctgatgc
     2281 agaccttgcg ggagtactat aaggtagacg tgagccgcta tgtgcgcatc aacttttcca
     2341 tcttcgagac cggggtcacc gatatcgggg gcgtggaggt ggagctgacc caggccgagg
     2401 ccgactatat gaacgggatc gcgggggaga agaaatggtc cgccggcccc gcgcggctgg
     2461 acggccccac ggctctggtc tacgcccgca tgcgccacct ggacagcgac tggcagcggg
     2521 tggagcgcca gcgcaaggtg ctgcaggccg cggcggacca gatcgcgacc ctgagcccgg
     2581 ccaagatcaa cctgatcgcc gacaagctgc tgcccatgat cgagaccaat ctgaccaacg
     2641 gcgagctgtg gcagctggcc tttaagatgc ccgccctgct gggcagcgac gccgcccaac
     2701 tgacggtgcc ggacgaggac gcctgctggg gcatcaaact gtcgtcgggg cagagcctga
     2761 tcggctgtga ttttgccgct gaggctgagc ggctggaccg ctttttgacc ggcgcggcgg
     2821 gttgagctct gcgcagggta ttgggcaatt tcacaaaaaa tgcggccttt cttgcggcgg
     2881 gatcgccaaa aacggccgcc gcggcttgcc gcaggcgggc ggatgtgcta tcatatgggg
     2941 cacaaagaaa cgttccgcag gagaaaaaga gcgcggggag agcttccttg atgaggcccc
     3001 ccgcgctctc tgttttcttc cccggagaaa agaggcggat atgaaacgga atttgaagcg
     3061 ccttggcatc gccctgggtg tgctgctggg cgcggccgtg ctggcggtgg ggggctatgt
     3121 gctgtacatg gtgctgcagt acgaccgcat ccccgaccac cagcccctca cggtggaaaa
     3181 cccgcagacc gcgcagctgt gccgggggca ggcctacact gcggcgacct acaacatcgg
     3241 ctttggcgct tacgacccgg acttctcctt tttcatggac accggcacca tgaaagacgg
     3301 cacgcccgtg cagggcgagc acggcagggc ccagagcgag cagatcgtcc tcaaggacac
     3361 tcagggcgcc atcgacacgc tgcaggggct cggcccggat ttttgcctgc tgcaggaggt
     3421 ggacaccgac gccacccgca gcttccacat cgaccagtcc cagatggtgc ggcaagcctt
     3481 tccccagtat ggggcggtgt ttgcctccaa tttccactcc gcctatcttg cctatccgct
     3541 ccacgagatg cacggcagcg tgcaggcggg gctgctcacc ctgtcccgct atgcggtggc
     3601 gcaggccgag cggcgcagtt atccggtgga cgagagcttc ccgacaaagt tttttgatct
     3661 ggaccgctgc ttttccgtac agcgcctttc ggtggcgggc ggcggcgagc tggtgctgat
     3721 caacagccac atgtccgctt atgacgaggg cggcaccgtg cgcgcggccc agctggagct
     3781 gctgggcggc gtgctgcggg aggagcggga aaagggcaac tatgtgattg tgggcggcga
     3841 tttcaaccac gcgctctgcg gcacggtgca ggcttttgcc agcgggcagc agatcccggc
     3901 ttgggtgttt gagttcagcg acagcgacct gcccgagggc tttcatgtgg tggaggcgga
     3961 aaacctgacc gaggtgccca cctgccgcag cagtgatatg ccctacaccc cgggcgtcaa
     4021 ctacacggcg gtgctggacg gctttatcgt cagcgacaac ctgacggcaa cggcgcggaa
     4081 cgtggacacc ggctttgcct acagcgacca caacccggtg ctgctcacct ttgtgctggg
     4141 gtgaggtcgg cgcggggaaa aggcggacaa caaaacagcg ggcgcgggga gtcgtttccc
     4201 cgcgcccgct gtttttggtc cgtcgctcat tcgccgtcgg gacggcccgg ctgctcgggg
     4261 cgctcgcgct cgaacagcat ggggatggcc acaagggcgg ccaggcccac gatggtgaag
     4321 acgatacggc tgaagatcga ggccgtgccg cccaaaagcc agcccacgca gtcaaagcgg
     4381 aacaggccca ccagccccca gttgaggccg ccgatgataa gcagtgccag acatattttt
     4441 ttgatcatat cgcaattcct ccacaggcgg gatatggccc gccaagattc taggtcctgc
     4501 ccatagaata cccgcttttg gcggggatat gcggcgcttt tgcgcgggca cggttgcaat
     4561 gtggggccct tttggggtat agtaatacat atctgacaac cacagcggcc ggcgctttgc
     4621 gccggctgcg aaaaagcaca gagaaagagg tataaaaatg acgatcacaa aagagaccat
     4681 catcggcgat atcctggacg cggcccccga gaccgcgcct ctgttccttg agatcggcat
     4741 gcactgcctg ggctgcccct cctcccgcgg cgagaccatc gcgcaggcct gctatgtgca
     4801 cggcaccgac gcggacgccc tggtcgaaaa gctgaacgcg gccgtcgccg gcaagtaatg
     4861 cagccgctga ggcccggcgc gcggctcgcc gcggccgccg ggctggtgcg ccccggcagc
     4921 cgcgcggccg acatcggctg cgaccacggc cgtctggcgg cctttcttgt cgagagcggc
     4981 cgctgcccct tcgtggtcgc cgccgacctg cggcccggcc cgctggaaaa agcgcgccgc
     5041 ctggccgccg cccgcgggct ggagggccgg gtcgactgcc gcctcggcga cgggctgtcg
     5101 gtgctgcgcc cgggcgaggt ggaggacatc gtggtcgccg gtatgggcgg ggagaccatc
     5161 gctgagatcc tggccgccgc gccctgggtg cggcggccgg gctgcaacct ggtgctgctg
     5221 cccgccagcg gccagccgca gctgcggcgc tggctgtgcg aaaacgggtt tgccctgctg
     5281 cgggaggtcc cggtgacgga ggggcgctat tgctatacgg tcctgcaggc ggcctatacg
     5341 ggcgagcgcg tctcaccggg gccgcttttc tgctggctgg gcctgctgcg cgggcagcgc
     5401 ggcccggcgg caaaggcgta ttttgccaag ctgctgcggc agctggagaa agagctgtgc
     5461 ggccgcgagc gggcggggga aaccggcctg gaggggctgc ggcggctgac ggaggagatc
     5521 ggaaaggagc tggcggagtg ccgagagtag aggagatccg cgcgtttttg gatgagctgg
     5581 cgccgcaggc gctggcggag gactgggaca atgtgggcgc gctggtggat tgcggcagca
     5641 acgtcaccag cgtgatgatg gcgctggaca tcaccgatga ggtggtgacc gaggcggaga
     5701 tggcaggctg ccagctcatc gtctcccacc atccggtcat ctttcacccg ctgcggtcgg
     5761 tgagccggtc ggacgtggcg ttccggatgg tcaaaaagag catcagcggc atctgtatgc
     5821 acaccaatat ggacgccgcc gacggcgggg tgaacgatat tttggccatc atcttcggcc
     5881 tgcaggaggt caaaaagctg gacggcatgg gccgggtggg ccgcctgcgc gcgcctaccg
     5941 gcgcggcgca gctggccgcg ctctgccggg agaagttcgg cgcggcggtg aagtacgtgg
     6001 acgcgggcag gccggtttcc acgctggccg tggtgggcgg ctcggggggc gatttcatcc
     6061 ccgccgcgct ggcggcgggc gccgacagcc tgctcaccgg ggaga
//
LOCUS       DBMDYA010000073         6081 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3294_length_6081_cov_1.741973, whole genome shotgun sequence.
ACCESSION   DBMDYA010000073 DBMDYA010000000
VERSION     DBMDYA010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_6081_cov_1.741973"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            242..1588
                     /gene="glmM"
                     /locus_tag="ACZPFK_02860"
     CDS             242..1588
                     /gene="glmM"
                     /locus_tag="ACZPFK_02860"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1405284.1"
                     /translation="MARLFGTDGVRGIAGEDLTPGLAMLIGRGAATVLTAQTSHRPRI
                     LIGKDTRISGDMLEAALSAGLCSVGADVELLGVIPTPAVAYLVRKYGADAGIVISASH
                     NPMEFNGIKIFNGDGYKLPDEVEDEIEHLVRNKAEVKLSTGAGVGRVTRRENAVEDYV
                     EYLAGCLQANLSGLRVAVDCANGAAAAPAQLLFARLGAECHFIGVEPDGVNINEGCGS
                     THIGNLAKYVVEHGLDCGVAFDGDADRCLAVDEKGNEIDGDKIIAVLAKHMREQGALP
                     GDTAVVTVMSNLGFMQYMRETLGLKIATTAVGDRYVLEEMRAKGYAIGGEQSGHVILL
                     QHSTTGDGELTAGTLLNILAATGGRMSALTGVYEKFPQVILNVIATPDEKARFKEDEY
                     IAGFIESQQQGLMGQGRVLVRVSGTEPYIRVMVEGRDQEAIAQAAQLIVDMIEKRK"
     gene            1641..2516
                     /locus_tag="ACZPFK_02865"
     CDS             1641..2516
                     /locus_tag="ACZPFK_02865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1405285.1"
                     /translation="MRLAENWKEYELLDATGGNRLERWGETILVRPDPQVVWRFEEAS
                     PLWRQADAVYHRSPSGGGRWERRRRFADEWTIGYGSLRLKVSPTGFKHTGIFPEQGVN
                     WDAYRRIIETAGRPVRVLNLFGYTGGATLACAAAGASVCHVDASKGMVAWARENAALS
                     GLSQRPIRWIVDDCGKFVQREARRGSRYDAVIMDPPSYGRGPSGEVWKLEDSIFELVT
                     ACGAILTEEPLFFAVNSYTAGLSPAVMEYMLRRMLCPRCGGRTVSDEIGLPVTTGGAL
                     PAGATSFWLAESAEL"
     gene            3015..3872
                     /locus_tag="ACZPFK_02870"
     CDS             3015..3872
                     /locus_tag="ACZPFK_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1405286.1"
                     /translation="MENQMVRAKDVVFRYSDQGRAALDGVSMEVRRGEFVAVLGANGC
                     GKSTLAKHFNAILLPEQGEAFVEQYNTKEEEHLYDIRQRVGMVFQNPDNQIVATVVEE
                     DVAFALENLGVPPDEMRGRVDEAMEMAGIYKHREKAPHKLSGGQKQRVAIAGVIAMRP
                     DCLVMDESTAMLDPIGREKVMRTVKKLNDEYGITVVMITHYMEEAVQADRVIVMSRGR
                     IELEGTPQEVFRDSARIRALKLDVPQAKELCDLLREAGVDLPPDIISADECAGALAAL
                     LSGAARAKK"
     gene            3966..4841
                     /locus_tag="ACZPFK_02875"
     CDS             3966..4841
                     /locus_tag="ACZPFK_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1405287.1"
                     /translation="MASVIRVEHLTYIYGEGMPDATTALDDISFEIEKGEFLGVIGPT
                     GCGKSTLITHFNGLNRPTRGRLQIDGEDIWADPKKIRQYRFKVGLVFQYPEYQLFEET
                     VRKDIGFGPRNMGLPDEEVAQRVVEAARFCGLDESVLDRSPFELSGGQKRRAAIAGVI
                     AMRPEVLVLDEPAAGLDPEGREMILSQVKRYHRETGTTVVLVSHSMEDIAKYADRVLV
                     LQAGKVAMLDTTANIFARAEELVSYGLSVPEVTKIFLKLREMGLDIPIDVYTVKYARD
                     TILNALAQRGGEGRA"
     gene            4834..5649
                     /locus_tag="ACZPFK_02880"
     CDS             4834..5649
                     /locus_tag="ACZPFK_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1405288.1"
                     /translation="MLRDITIGQHFPGNSVIHRMDPRAKLLLTIAYIVILFVGSNPIG
                     LALSALFLVVAYGVARIPLKLIGKSLRPILPIILFTAVLNLFFVPGDPIFSWWVITVT
                     WQGLYNVLVLSVRIVCLIAGTSLLTYTTSPIVLTDALERLLKPFARLHLPVHELAMMM
                     TIALRFIPTLIEETDKIMSAQKARGAELDTGKLTDRIKALIPVLIPLFISAFRRADEL
                     AMAMECRCYHGGEGRTRLRQLHLGGMDFVLVAVCAAVFTLIGCSGMFWGLLGL"
     gene            5664..>6081
                     /locus_tag="ACZPFK_02885"
     CDS             5664..>6081
                     /locus_tag="ACZPFK_02885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0106029 - tRNA pseudouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR1405289.1"
                     /translation="MSLAYQGTAYHGFQVQKNALTVAEVLQNAIRAALGSREEIKGCS
                     RTDAGVHALAYCVSFFTDSALRCEKLPLALNAHLPADIRVFSAREVGPDFHARYSCTG
                     KAYLYRVRNAPAASPFSHQLAWRVWPRLELEPMRRAA"
BASE COUNT         1081 a         1902 c         2148 g          950 t
ORIGIN      
        1 cccgcgccgg gcagggggcg cccgccgacg ggccgcgcca gcagagcttt gacgatctgg
       61 gcgcgccgga gcagtaaaat cggttgggcg cgggcggcgc gcacatcacc gcgccgcccg
      121 tacataaaat aaatgaaggc tctttttagc gggcgcggcg caggccgccc cgcccgcagg
      181 ggccgcaacc ggccgaggcc ggcatccggg gcgacccgcc ccacgattca ggaggaagat
      241 tatggcaaga ctgtttggaa ccgacggcgt ccgcggcatc gcgggcgagg acctcacccc
      301 cggcctggcg atgctcatcg gccgcggcgc ggcgacggtg ctcaccgcgc agaccagcca
      361 ccggccccgc atcctcatcg gcaaggacac ccgcatctcc ggcgacatgc tggaggccgc
      421 tctctcggcc ggcctgtgca gcgtgggcgc ggacgtggag ctgctgggtg tgatccccac
      481 cccggccgtg gcctatctgg tgcgcaagta cggcgcggac gcgggcatcg tgatctcggc
      541 cagccataac ccgatggagt tcaacggcat caagatcttc aacggcgacg gctacaagct
      601 gcccgacgag gtggaggatg agatcgagca cctggtgcgc aacaaggccg aggtcaagct
      661 gtccaccggc gccggggtgg gcagggtcac ccgccgcgag aacgcggtgg aggactatgt
      721 ggagtatctg gcgggctgcc tgcaggcaaa cctctccggg ctgcgggtgg ccgtcgactg
      781 cgccaacggc gcggcggcgg ccccggcgca gctgctgttt gcccgcctcg gggccgagtg
      841 ccatttcatc ggcgtggagc cggacggcgt gaacatcaac gagggctgcg gctccaccca
      901 catcggcaat ctggcgaagt atgtggtgga gcacgggctc gactgcggcg tggcctttga
      961 cggcgacgcg gaccgctgcc tggcagtgga cgaaaagggc aacgagatcg acggcgacaa
     1021 gatcatcgcc gtgctggcaa agcacatgcg cgagcagggg gccctgccgg gcgatacggc
     1081 ggtggtcacc gtcatgtcca acctgggctt catgcagtat atgcgggaga ccttgggcct
     1141 caagatcgcc accaccgcgg tgggcgaccg ctatgtgctg gaggagatgc gcgccaaggg
     1201 ctatgccatc ggcggcgagc agagcgggca cgtcattctg ctgcagcact ccaccaccgg
     1261 cgacggagag ctgacggcgg gcacgctgct gaacatccta gcggccacgg gcggcaggat
     1321 gagcgccctc accggcgtgt acgaaaagtt tccgcaggtc atcctcaacg tgatcgccac
     1381 gccggacgaa aaggcgcggt tcaaggagga cgaatatatc gcaggcttta tcgagagcca
     1441 gcagcagggc ctgatgggcc aggggcgggt gctggtgcgc gtctccggca ccgagcccta
     1501 tatccgcgtc atggtggagg gccgggatca ggaggccatc gcccaggccg cgcagctgat
     1561 tgtagacatg atcgagaaaa gaaagtagag ccgcctgagg gcgccgcgag gcgccccggg
     1621 cggagagaaa ggggccgcat atgcgtcttg ccgagaactg gaaagaatac gagctgctgg
     1681 acgccaccgg cggcaaccgg ctggagcgct ggggcgagac catcctggtg cgcccagacc
     1741 cgcaggtggt ctggcgcttt gaggaggcca gccccctgtg gcggcaggcg gacgcggtct
     1801 atcaccgcag ccccagcggc ggcggccggt gggagcgccg ccgccgcttt gccgacgagt
     1861 ggacgatcgg ctacggcagc ctgaggctga aggtgtcgcc caccggcttc aagcacaccg
     1921 gcatcttccc cgagcagggg gtcaactggg acgcctaccg ccgcatcatc gagacggccg
     1981 ggcggccggt gcgggtgttg aacctgttcg gctacacggg cggggcgacg ctggcctgcg
     2041 cggccgcagg ggccagcgtc tgccacgtgg acgcctccaa gggcatggtg gcctgggcgc
     2101 gggagaacgc ggccctctcc ggcctgtcgc agcgccccat ccgctggatc gtggacgact
     2161 gcggcaagtt cgtgcagcgg gaggcccgcc gcggcagccg ctacgacgcg gtcatcatgg
     2221 acccgcccag ctatgggcgc ggcccctccg gcgaggtctg gaagctggag gacagtatct
     2281 ttgagctggt gaccgcctgc ggcgcgatcc tgaccgagga accgctgttt tttgcggtga
     2341 acagctacac cgccggcctc tcgccggcgg tgatggagta catgctgcgc cggatgctct
     2401 gcccgcgctg cggcggccgc accgtcagcg acgagatcgg cctgccggtg acgacaggcg
     2461 gcgcactgcc cgccggagcg accagctttt ggctggcgga gagcgcggaa ctgtaatggg
     2521 agcaaaagct ttgtcgcgcc gccggacgtg aagccgtcgc tgggggagaa cggcgcagcc
     2581 gttctccttg ggacggccgg gagactcaag gttatgacgg gacagaacac cgtcccgcgc
     2641 ggggcattac cggcaggggc gaccagcttt tggctggcgg agggcgcggg actgtgatga
     2701 gaacaaaaac tccgccgtgc cgccggacgt aaagctgtcg ctgggggaga acggcgcagc
     2761 cgttctcctt gggacggccg ggagactcaa ggttatgacg ggacagaacg ccgtcccgcg
     2821 cggggcgtta ccggcagggg cgaccagctt ttggctggcg gatggcgtgg aactgtgaaa
     2881 aaacaaaaaa accgccaccg cccgaatgcc cgcgaggggc atgtcccgcg cagatggaaa
     2941 acagatcaaa aaatctccag aaaagtccct gcatcgcctc tgggcgagcg ggagacaggg
     3001 agggaagctg aagaatggaa aatcaaatgg tccgggcaaa agacgtggtc ttccgctatt
     3061 ccgatcaggg ccgcgcggcg ctggacgggg tctcgatgga ggtgcggcgc ggggagttcg
     3121 tggccgtgct gggggccaac ggctgcggca agtccacgct ggccaagcac ttcaacgcta
     3181 tcctgctgcc ggaacagggc gaggcttttg tcgagcagta caacaccaag gaggaggagc
     3241 acctctatga catccgccag cgggtgggca tggtgttcca gaacccggac aaccagatcg
     3301 tcgccaccgt cgtggaagag gacgtggcct ttgcgctgga aaatctgggt gtgcccccgg
     3361 acgagatgcg gggccgggtg gacgaggcca tggagatggc cggcatttac aagcaccgtg
     3421 aaaaagcccc gcacaagctg tccggcgggc agaaacagcg ggtcgccatc gcgggggtca
     3481 tcgccatgcg gcccgactgt ctggtgatgg acgagtccac cgccatgctg gaccccatcg
     3541 gccgcgaaaa ggtgatgcgc accgtgaaaa agctcaacga cgagtacggc atcacggtgg
     3601 ttatgattac ccattatatg gaagaggccg tgcaggccga ccgggtgatc gtgatgagcc
     3661 gcgggcgcat cgagctggag gggaccccgc aggaggtttt tcgggacagc gcgcgcatcc
     3721 gggcgctcaa gctggacgtg ccgcaggcca aggagctctg cgatctgctg cgggaggcgg
     3781 gcgtggacct gcccccggac atcatctcgg cggacgagtg cgccggggcg ctggcggcgc
     3841 tgctgtccgg cgcggcccgg gcaaaaaaat agtcggcgcg gcggggcaaa ggccccgcgc
     3901 ccggcagaga aagggcgggc tgccagccgc acggcggcgg acctctgccc ggaacaggtg
     3961 gtaacatggc atcagtgatc agggtcgaac atttgaccta tatttacggc gagggtatgc
     4021 ccgacgccac gacggcgctg gacgacatca gcttcgagat cgaaaaaggt gaatttttgg
     4081 gggtgatcgg gccgacgggc tgcggcaagt cgaccctgat cacccatttc aacggcctca
     4141 accggcccac gcggggcagg ctgcagatcg acggcgagga catctgggcg gaccccaaaa
     4201 agatccggca gtaccgcttt aaggtggggc tggtgttcca atatcccgag taccagctct
     4261 ttgaggagac ggtgcgcaag gatatcggct ttgggccgcg caacatgggc ctgccagacg
     4321 aggaggtggc ccagcgggtg gtggaggccg cgcgcttctg cggcctggat gagtcggtgc
     4381 tggaccgctc gccctttgag ctctcgggcg ggcagaagcg ccgggccgcc atcgcgggcg
     4441 tcatcgccat gcgccccgag gtgctggtgc tggacgagcc cgccgcgggc cttgacccgg
     4501 agggccggga gatgatcctc agccaggtca agcggtatca cagggagacc ggcaccactg
     4561 tggttctggt gagccactcg atggaggata tcgccaagta cgccgaccgc gtgctggtgc
     4621 tgcaggcggg taaggtggct atgctggaca ccacggcgaa tatctttgcc cgcgccgagg
     4681 agctggtgag ctacggcctc tcggtgccgg aggtgaccaa aatcttcctc aagctgcggg
     4741 agatgggcct cgacatcccc atcgacgtct acaccgtcaa atatgcgcgg gacacgatct
     4801 tgaacgccct ggcgcagcgg ggaggtgagg gccgtgctta gggacatcac catcgggcag
     4861 catttccccg gcaactcggt catccaccgc atggaccccc gtgccaagct gctgctcacc
     4921 atcgcttaca tcgtcatctt gttcgtgggc tccaacccca tcgggctggc gctgtccgcc
     4981 ctgttcctgg tggtggccta cggggttgcc aggatccccc tgaagctgat cggcaaaagc
     5041 ctgcgcccca tcctgcccat catcctgttc accgcggtcc tcaacctgtt ttttgtgccc
     5101 ggtgatccca tcttcagctg gtgggtgatc acggtcacct ggcagggcct ctataatgtg
     5161 ctggtgctct cggtgcgcat cgtctgcctg atcgcgggca ccagcctgct cacctatacc
     5221 accagcccca tcgtgctcac cgacgcgctg gagcggctgc tcaagccctt tgcgcggctg
     5281 cacctgccgg tgcacgagct ggcgatgatg atgaccatcg ccctgcgctt catccccacc
     5341 ctgatcgagg agaccgacaa gatcatgagc gcgcagaaag cgcgcggcgc cgagctggac
     5401 accggcaagc tcaccgaccg catcaaggcg ctgatcccgg tgctgatccc gcttttcatc
     5461 tccgcgttcc gccgcgccga cgagctggcg atggccatgg aatgccgctg ttatcacggc
     5521 ggcgagggac gcacccggct gcggcaactg cacctgggcg gcatggactt tgtgctggtg
     5581 gcggtctgcg cggcggtgtt cacattgatc ggctgttcgg gcatgttctg ggggctgctg
     5641 gggctatgag gcggcatctg gctttgtccc tggcctatca gggcacggcg tatcacggct
     5701 ttcaggtgca gaaaaacgcc ctcacggtgg ccgaggtgct gcaaaacgcc atccgtgccg
     5761 cgctgggcag ccgggaggag atcaagggct gctcccgcac cgatgcgggc gtgcacgcgc
     5821 tggcctactg cgtgagcttt ttcaccgaca gcgccctgcg gtgtgaaaag ctgccgctgg
     5881 cgctcaacgc ccatctgccg gcggatatcc gcgtcttttc ggcgcgggag gtggggccgg
     5941 actttcacgc ccgctatagc tgcaccggca aggcctacct ttaccgggtg cgcaacgcgc
     6001 ccgccgcgtc tcctttcagc caccagctcg cctggcgggt ctggcccagg ctggagctgg
     6061 agccgatgcg cagggccgcg g
//
LOCUS       DBMDYA010000074         6067 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3303_length_6067_cov_1.672142, whole genome shotgun sequence.
ACCESSION   DBMDYA010000074 DBMDYA010000000
VERSION     DBMDYA010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3303_length_6067_cov_1.672142"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="ACZPFK_02890"
     CDS             <1..1063
                     /locus_tag="ACZPFK_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015995.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1405290.1"
                     /translation="LNGNDPNLVLLGAIPASLLALLVDLVLSVLEKAVTAEGLKSPDR
                     IVLRSRGQKRRRALVAAALCAVLLTVPVGSAAAKAVDAAQKKITVGTTNMTEAIILGY
                     LYEGLIEENTGIQVEERFNLNGSPLSFAALERGDVDMFTDYTGIIGPILLGLPTETDP
                     QKVYDDVVRLMRDEHDIFISQPLGFSNEYVISASPAAAEAYGLDSLSDLVDNAPFLRL
                     GCTTAFTQREDCLPKMESQFGIHFKEVIGLEGNIRYQAISSGEVEVTDAYSTDAMTVK
                     VDLAPLKDDLGFFPPYQAVNLVRRDTLDRYPELPPLLAKLDGAITTQEMARMNYEVDV
                     EGKNPKDVAYAFLAGKGLI"
     gene            1429..3183
                     /gene="kdpA"
                     /locus_tag="ACZPFK_02895"
     CDS             1429..3183
                     /gene="kdpA"
                     /locus_tag="ACZPFK_02895"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR1405291.1"
                     /translation="MTNQILQYVLYVAILVALAIPLGNYMYKVMRGEKTFLTRALRPV
                     EDGIYKLCRIDPEENMGWKQYALAAVLFSAFGFVFLFLLNVLQGFLPLNPAGAPGASW
                     HLAFNTTASFVTNTNWQAYSGETALSYLTQMLGLTVQNFVSAATGIAVMFALIRGFAR
                     VKEKGLGSFWADLTRVTLYILLPLSLVATLVLASQGVVQTFKAYDTVDLLEPVVIQQE
                     DGSELTVTKEVIPLGPAASQISIKQLGTNGGGFFGVNSAHPFENPTALSNLVEMTSLL
                     LIPVACCFTFGLGVRDKKQGRALFIAMMVLLTAFLVIAAVSEAASTPQLAQDGAVQTQ
                     ITGGQPGGNMEGKETRFGIAASTTWGVFTTAASNGSVNSMHDSYTPLGGMIEMLQMEL
                     GEVVFGGVGCGLYGMVGFAVLAVFIAGLMVGRTPEYLGKKIEPREMKMAVLVCLATPV
                     AILIGAGLATALPATAESLNNGGAHGFSEVLYAYSSAGGNNGSAFAGLNANTPFFNVS
                     IGLVMLFVRFVPLVATLAMAGSLVKKKKVAAGAGTLPTHDALFIFLLVFVVLLVGALS
                     FFPALALGPVAEFFQMMA"
     gene            3200..5278
                     /gene="kdpB"
                     /locus_tag="ACZPFK_02900"
     CDS             3200..5278
                     /gene="kdpB"
                     /locus_tag="ACZPFK_02900"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR1405292.1"
                     /translation="MNSKKKEQFATRDLIGRAVRDSFFKLAPGTQIKNPVMFLVYVSA
                     FLTSGLYLCSLAGVSDAGLHPAFILAVAVILWFTVLFANFAEAMAEGRGKAQADALRA
                     AKQDVDANRIPGPGRRGEITPVKSAALKKGDYVLVRAGEQIPADGEVAEGAASVDESA
                     ITGESAPVIREAGGDRSAVTGGTTVLSDCLVVRVTSEEGESFLDKMIAMVEGASRKKT
                     PNEIALEILLIALTIIFLLVTVSLFPFSQFSADQAGIANPTSVTALAALLVCLAPTTI
                     GALLSAIGIAGMSRLNQANVLAMSGRAIEAAGDVDTLLLDKTGTITLGNRQAVELLPV
                     DGARAEELADAAQLASLADETPEGRSVVVLAKEQFGLRGRDLAGLKASFVPFAAKTRM
                     SGIDYEEGEVRKGAADAVRAYVEARGGRVSGECQAVVDRIARAGGTPLVVAKNARVLG
                     VIHLKDIIKQGVKEKFADLRRMGIRTVMITGDNPLTAGAIAAEAGVDDFLAEATPEGK
                     LKMIRELQAKGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTK
                     LIDIVRIGKQLLMTRGSLTTFSIANDVSKYFAILPAMFMGLYPGLSALNIMGLSSPES
                     AILSAVIYNALIIVALIPLALRGVKYREVPAAKLLSRNLLVYGLGGLAVPFIAIKLID
                     MLITGLGLVA"
     gene            5299..5940
                     /gene="kdpC"
                     /locus_tag="ACZPFK_02905"
     CDS             5299..5940
                     /gene="kdpC"
                     /locus_tag="ACZPFK_02905"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HIR1405293.1"
                     /translation="MQALKKDTLRALLLVVVMTIVCGLAFPLLMTGVSQLLFRDKANG
                     SIIEVDGKKYGSSLLAQEFAGDEYLWGRVMNANASAFLLDESGNVVIAADGITGEPLY
                     YSGPSNKSPASEEYERRITERVERIEAAHPEKQGQPIPADLVTCSGSGLDPHISPDAA
                     EYQVARVANARGMSEDEVRAVIENYTEGRFLGIFGEPTVNVLKVNLALDGILK"
BASE COUNT         1071 a         1927 c         1971 g         1098 t
ORIGIN      
        1 tctgaacggc aacgacccca acctggtgct gctgggcgcc atcccggcat ccctgctggc
       61 tctgctggtg gacctggtgc tctccgtgtt ggagaaagcc gtcaccgccg agggcctgaa
      121 aagcccggat cggatcgtgc tgcgcagccg cgggcaaaag cgccgccggg cgctggtggc
      181 cgccgccctc tgcgcggtgc tgctgaccgt gccggtgggc tccgccgcgg ccaaggcggt
      241 ggacgccgcc cagaaaaaga tcacggtggg caccaccaac atgaccgaag ccattatttt
      301 gggctatctc tacgaggggc tgatcgagga gaacaccggc atccaggtgg aggagcggtt
      361 caacctgaac ggctcgccgc tgagctttgc ggcgctggag cggggggacg tggacatgtt
      421 caccgactac accggcatca tcgggcccat cctgctgggg ctgcccaccg agaccgaccc
      481 gcaaaaggtc tacgacgacg tggtgcgcct gatgcgggac gagcacgata tcttcatctc
      541 gcagccgctg ggtttttcca acgaatatgt gatcagcgcc agccccgccg ccgccgaggc
      601 ctatgggctg gacagcctgt ccgacctggt tgacaacgct ccgttcctgc gcctgggctg
      661 caccaccgct ttcacccagc gggaggattg cctgcccaag atggaatccc agttcggcat
      721 ccatttcaag gaggtgatag ggctggaggg caacatccgc tatcaggcca tcagctcggg
      781 cgaggtggag gtgaccgacg cctattccac cgacgcgatg acggtcaagg tggacctggc
      841 gccgctcaag gacgacctgg gcttttttcc accctaccag gcggtgaacc tggtgcggcg
      901 ggacacgctg gaccggtacc ccgagctgcc gccgctactg gcgaagctgg acggcgccat
      961 caccacccag gagatggccc gcatgaatta tgaggtggac gtggagggta aaaaccccaa
     1021 agacgtggcc tatgcctttc tcgcgggcaa agggctgatc tgagggcgcg tccgcgcgcg
     1081 gaaccaccaa aaaagagagc ggctcacgac gggccgctct ctttttgccc tcccgccctt
     1141 tcctccccga aagcggtcct gcgggggacg gcgggcgcga tcggtcccct ccacctttaa
     1201 cgcagtttta atcccagcac cggaaccctt atccctcttt tatagtcgat atgctataaa
     1261 tagtagacag agatttgggc ggatcgctca agccctgtcg ggcatttggt ccgagctttg
     1321 aggaagaggt gaagcatgca ctatgggtgt catcatggcg gtgatcggcg cggtatccct
     1381 gctgatgctg gtctatctga tctacgcgtt gttcagaggt gacgaaaaat gacaaatcaa
     1441 attttacagt acgtcctata tgtcgcgatc ctcgtcgccc tcgcaatccc cctgggtaac
     1501 tatatgtaca aggtgatgag aggggaaaag acctttctca cccgcgcgct gcggccggtg
     1561 gaggacggca tctacaagct ctgccgcatt gaccccgagg agaacatggg ctggaagcaa
     1621 tatgcactgg cggcggtgct gttcagcgcg ttcggtttcg tgttcctgtt tctgctcaat
     1681 gttttgcagg gctttctgcc cctcaatccg gccggggcgc ccggcgccag ctggcatctg
     1741 gcgttcaaca ccaccgccag ctttgtgacc aacaccaact ggcaggccta ctccggcgag
     1801 acggcgctgt cctatctgac ccagatgctg ggcctgacgg tgcagaactt tgtttccgcc
     1861 gccaccggca tcgcggtgat gttcgcgctc atccgcggct ttgcgcgcgt caaggaaaag
     1921 ggtctgggca gcttttgggc cgacctgacc cgcgtcaccc tgtacatcct gctgccgctg
     1981 tccctcgtgg cgaccctggt cctcgcctct cagggcgtgg tgcagacttt caaggcctac
     2041 gacacggtgg atctgctgga gccggtggtg atccagcagg aggacggcag cgagctcacc
     2101 gtcaccaaag aagtcatccc cctgggcccg gcggccagcc aaatttccat caagcagctc
     2161 ggcaccaacg gcggcggctt ttttggggtc aactccgctc atccgtttga aaaccccacc
     2221 gccctctcca acctggtcga gatgacttcg ctgctgttga tcccggtggc ctgctgcttc
     2281 actttcgggc ttggggtgag ggataaaaaa cagggccggg cgctgtttat cgccatgatg
     2341 gtcctgctga cggcttttct cgtcatcgcc gccgtttcgg aggcggcgtc caccccgcag
     2401 ctcgcccagg acggcgcagt gcagacccag atcaccggcg gacagcccgg cggcaatatg
     2461 gagggcaagg agacgcgctt tggcattgcc gcctccacta cctggggcgt ctttaccacc
     2521 gcggcctcca acggctcggt caactcgatg cacgacagct acaccccgct cggcggcatg
     2581 atcgaaatgc tgcagatgga gctgggcgag gtggtgtttg gcggtgtggg ctgcggcttg
     2641 tacggcatgg tgggctttgc ggtgctggcg gtgttcatcg ccgggttaat ggtgggccgc
     2701 acgccggagt atctgggcaa aaagattgag ccccgcgaga tgaagatggc ggtgctcgtc
     2761 tgcctcgcta ccccggtggc gatcttgatc ggcgccgggc tcgccaccgc cctgcccgcc
     2821 acggcggagt cgctgaacaa cggcggggcg cacggatttt ccgaggtgct ctacgcttac
     2881 tcgtcggcgg ggggcaacaa cggctccgcc tttgcgggtc ttaacgccaa caccccgttt
     2941 tttaacgtct ccatcggcct ggtgatgctg tttgtgcgct ttgtgccgct ggtcgccacc
     3001 ctggcaatgg ccggctcgct ggtgaaaaaa aagaaagtcg ccgccggcgc gggcaccctg
     3061 cccacccacg acgcgctgtt catctttttg ctggtctttg tggtgctgct ggtgggcgcg
     3121 ctcagctttt tccccgcgct tgccctgggg ccggtcgccg aattctttca gatgatggct
     3181 taaaggagtg aggtcgatta tgaattctaa aaagaaagag caatttgcaa cgcgggacct
     3241 catcggccgg gcggtgcggg attctttttt caagctggcc cccgggaccc agatcaaaaa
     3301 ccccgtcatg ttcctggtct atgtgtccgc gtttttgacc agcgggcttt atctctgctc
     3361 gctggccggc gtctccgacg ccgggctgca cccggccttt atcctggcgg tggccgtgat
     3421 cctctggttc acggtgctgt tcgcaaactt tgccgaggcg atggccgagg gccgcggcaa
     3481 ggcccaggcc gacgcgctgc gggcggcaaa gcaggacgtg gacgcgaatc gcatccccgg
     3541 ccccggccgc aggggggaga tcacccccgt caaaagcgcc gccctgaaaa agggcgacta
     3601 cgtgctggtg cgggcgggcg agcagatccc cgcggacggc gaggtggccg agggggcggc
     3661 cagcgtggac gaatccgcca tcaccggcga atccgcaccg gtcatccgcg aggcgggcgg
     3721 cgaccgcagc gcggtcaccg gcggcaccac cgtgctctct gactgcctgg tggtgcgggt
     3781 caccagcgag gagggcgaga gctttctcga caagatgatc gccatggtgg agggggcctc
     3841 ccgcaaaaag acccccaacg agatcgcgct ggaaatttta ttgattgcgc tgaccatcat
     3901 ctttttgctg gtcaccgtct cgctgttccc gttttcccag ttctccgccg accaggcggg
     3961 catcgcaaac ccgacctccg tgacagccct ggcggcactg ctggtctgcc tcgcgcccac
     4021 caccatcggg gcgctgctct ccgccatcgg catcgcgggc atgagccgcc tcaaccaggc
     4081 caatgtgctg gccatgagcg gccgggccat cgaggccgcc ggcgatgtgg ataccctgct
     4141 gctggataag accggcacca tcaccttggg caaccgtcag gccgtcgagc ttctgccggt
     4201 ggacggcgcc cgggcggagg aactggccga cgcggcgcag ctggcctcgc tggcggatga
     4261 gacgccggag ggccgcagcg tggtggtgct ggccaaagag cagtttggcc tgcggggccg
     4321 cgacctggcc ggcctgaaag cctcctttgt cccctttgcc gccaagaccc gcatgagcgg
     4381 catcgattac gaggagggcg aggtgcgcaa gggcgccgcc gatgcggtgc gcgcctatgt
     4441 cgaggcccgg ggcggccgcg tttccggcga gtgccaggcc gtggtggacc ggatcgcccg
     4501 cgccggaggc accccgctgg tggtggcgaa gaacgcccgg gtgctgggcg tcatccacct
     4561 gaaggacatc atcaagcagg gcgtcaagga aaaatttgcc gacctgcgca ggatgggcat
     4621 ccgcaccgtc atgatcaccg gcgacaaccc gctgaccgcg ggcgccatcg ccgcggaggc
     4681 cggggtggac gattttctgg ccgaagccac gcccgagggc aagctgaaga tgatccgcga
     4741 gctgcaggca aagggccatc tggtggccat gaccggcgac ggcaccaacg atgcgccggc
     4801 actggcccag gccgacgtgg cggtggccat gaacaccggc acccaggcgg ccaaggaggc
     4861 gggcaacatg gtggacctgg actcttcgcc caccaagctg atcgacatcg tgcgcatcgg
     4921 caaacagctg ctgatgaccc gcggctccct caccacgttc agcatcgcca acgacgtgtc
     4981 gaagtacttc gccatcctgc ccgccatgtt tatgggcctg tatcccggcc tctctgccct
     5041 gaacatcatg ggcctgtcca gcccggagag cgccatcctg tccgccgtca tctacaacgc
     5101 gctgatcatt gtggcgctca tcccgctggc cctgcgcggg gtcaagtacc gggaggtgcc
     5161 cgcggcaaag ctgctcagcc gcaacctgct ggtctacggg ctgggcgggc tggcggtccc
     5221 gttcatcgcc atcaaactga tcgacatgct gatcaccggc ctcggcctgg tggcctgacc
     5281 gaaaaggggg agcaaataat gcaagctttg aaaaaggata cgctgcgggc gctgctgctc
     5341 gtcgtcgtga tgacgatcgt ctgcgggctc gcctttccgc tgttgatgac cggcgtctca
     5401 cagctgctgt tccgcgacaa ggccaacggc agcatcattg aggtggacgg caaaaaatac
     5461 ggcagcagcc tgctggcgca ggagtttgcc ggcgacgaat acctgtgggg ccgtgtgatg
     5521 aacgccaacg cctccgcctt tctgctggac gaaagcggca acgtcgtcat cgccgccgac
     5581 gggatcaccg gggagccgct ctactactcc ggcccctcca acaagagccc ggccagtgag
     5641 gaatatgagc ggaggatcac cgagcgggtg gagaggatcg aggccgccca cccggaaaag
     5701 cagggccagc ccatcccggc ggacctggtc acctgctccg gctcgggcct ggacccccac
     5761 atctcgccgg acgcggccga ataccaggtg gcccgcgtgg cgaacgcgcg ggggatgagc
     5821 gaggacgagg tgcgcgccgt catcgaaaac tataccgagg gacgctttct gggcattttc
     5881 ggcgagccca ccgtcaatgt gctgaaagtg aacctggcgc tggacggcat cctgaaatga
     5941 cgggacaggg ataaaggaaa agccccgccc gcctcacggc tgtgaggcgg gcggggcttg
     6001 tttgcactgt gacaggcgcg gggccgtacg ccgggggcga tccggtgccg ggagccgcac
     6061 aagaacg
//
LOCUS       DBMDYA010000075         6062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3307_length_6062_cov_1.630431, whole genome shotgun sequence.
ACCESSION   DBMDYA010000075 DBMDYA010000000
VERSION     DBMDYA010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3307_length_6062_cov_1.630431"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="ACZPFK_02910"
     CDS             <1..1360
                     /locus_tag="ACZPFK_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1405294.1"
                     /translation="VRLETSPEDIVGMQVSQGILTVRGGMTSHAAVVARGMGTCCVCG
                     CGNSNDVVVDYEAKTFTINGKVFHEGDFISIDGTTGNIYEGQVATVAATENAELNRFM
                     GWADKYRQLQVFANADNPHDAQQGSDFGAEGIGLCRTEHMFFAEDRIKAMREMIVADN
                     TADREKALEKILPYQQGDFEGLYEVMGERPVTIRYLDPPLHEFLPTKEEDIKEIAGEL
                     GITVEKLHNVIDSLHEFNPMMGHRGCRLAVSYPEIAVMQTRAVINAALNVSKKKGIKI
                     VPHIMIPLVGEVKELKFVKDVVVKTADEIIAAAGVEMKYEVGTMIEIPRAALTADEIA
                     KEAEFFSFGTNDLTQMTFGFSRDDAGKFLDYYYENKIYELDPFAHLDQNGVGKLVEMA
                     AKLGRQTRPDLGLGICGEHGGDPTSVEFCHKVGLDYVSCSPFRVPIARLAAAQAAIKF
                     PR"
     gene            complement(1575..1964)
                     /locus_tag="ACZPFK_02915"
     CDS             complement(1575..1964)
                     /locus_tag="ACZPFK_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HIR1405295.1"
                     /translation="MPDKEPRFFACTKCGVLIEEIAGNKTHYSCGGKELEELTPNTSE
                     GAQEKHLPVVERSGDMVTVKVGSVFHPMSAEHSIQWVYLQTEKGCQRVDLCCEGEPVA
                     VFALAPGDRPLAAYAYCNLHGFWKTVL"
     gene            2269..2682
                     /locus_tag="ACZPFK_02920"
     CDS             2269..2682
                     /locus_tag="ACZPFK_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405296.1"
                     /translation="MRVHAGQKKRKAAPQKAQGWKRLPVLPVRRKVKSVGGLRKRLGC
                     RETARGKLGASERRGQIRQVCRSEPVDIYFLLELRKRAKKHRQAEREREKRVARGGKN
                     RKTKGKAEEDKYELLLHDDSFGLKAIDYLTRKNPK"
     gene            2851..3120
                     /locus_tag="ACZPFK_02925"
     CDS             2851..3120
                     /locus_tag="ACZPFK_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405297.1"
                     /translation="MKKIMTIALALTLALGLVACGSDSSSSTPASSSAVSSSVSAPSS
                     VADSSEAGSSEADSKDTSSSAADSSEASSSEAGSSSAASSSKVEE"
     gene            3358..5862
                     /locus_tag="ACZPFK_02930"
     CDS             3358..5862
                     /locus_tag="ACZPFK_02930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     diguanylate cyclase"
                     /protein_id="HIR1405298.1"
                     /translation="MKRKSGRVLASLLMAFLFLFSAVPAQGAGETGRTIRVAYPYQAG
                     LTEVDEQGNYSGYTYEYLQEIAQYTGWSYEFVEVPGTPDESLTQLMEMLENGEVDLMG
                     GMLYSASMAELYDYSGYSYGVVETVLQVLQEDTRDLVVDSQTQQTFRVAVPSSSKQRL
                     KELQEYCDMNLIELVPVLCADKAEQLQALREGRADALLATSMDYTPGLRTIARFAQKP
                     FYFVLSKKCDQGIMQELNAAIADVERADPTYSTALFEKYFMPKNDTAVLTDAEQAYLA
                     AAGTLRVGVMDDQPPFQYREEGAFAGIAVDLLEVIAQETGLQFELVPVKGTAALEELL
                     EAGELDLVAGMTYDYDEANRLGLAMSRPYLSAQYVQLQKTSSPESSGGRLAASVNNNY
                     GSAAASEVLWYDSISECVAAVDRGEADFTFVDAYTGQYYVNTSAYADLKVVSQLSGQR
                     QICFGLVKPTSRQLLSVLNKELAVLPAAHMQEIVYVNTLYTPEISLRELLRDHPAVAV
                     SLVAGVLLAVIAVLLIFLRQRNASSRRLALELKKHLQVYGMVSDAFFEYDYAAQRLII
                     STPHAGEDGEAELKSYFLGGTEEIDPFIGSFVDFVRQSEDGMHEKQLPRDNGQLHWLR
                     VATRTIHDDHGKRAYTVGRISDIEEERRAQQELLEKAQRDSLTGLFNTAAGRQRSEEL
                     LAGLRGGNRDALILLDIDYFKEVNDRYGHRRGDWVLTRVAEVLRSSFRPEDVIGRPGG
                     DEFVVYLRGARELAALGERCGTLCRRIRELSRDGLHLSVSIGAALAGPGDSYEAVYER
                     ADQALYRAKDAGRDRYILVPWPGDTQGVAGDEALRG"
     gene            complement(5937..6062)
                     /locus_tag="ACZPFK_02935"
     CDS             complement(5937..6062)
                     /locus_tag="ACZPFK_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1405299.1"
                     /translation="MAEGVEQKPQLDFLQAAGCDLIQGYYFSRPLAPAAFAALLS"
BASE COUNT         1259 a         1711 c         1942 g         1150 t
ORIGIN      
        1 cgtccgcctc gagaccagcc ccgaggacat cgtgggcatg caggtctccc agggcatcct
       61 gaccgtgcgc ggcggcatga ccagccacgc ggccgtcgtg gcccgcggca tgggcacctg
      121 ctgtgtctgc ggctgcggca acagcaacga cgttgtggtg gactatgagg ccaaaacctt
      181 taccatcaac ggcaaggtct tccacgaggg cgattttatc tccatcgacg gcaccaccgg
      241 caacatctat gagggccagg tcgccaccgt cgccgccacc gagaacgctg agctgaaccg
      301 cttcatgggc tgggccgaca agtatcgtca gctgcaggtc ttcgccaacg cggacaaccc
      361 gcacgacgcc cagcagggct ccgacttcgg cgccgagggc atcggcctgt gccgcaccga
      421 gcacatgttc ttcgccgagg accgcatcaa ggccatgcgc gagatgatcg tcgccgacaa
      481 caccgccgac cgcgaaaagg cgctggagaa gatcctgccc tatcagcagg gcgacttcga
      541 gggcctgtat gaggtcatgg gtgagcgccc ggtcaccatc cgctacctgg atccgcctct
      601 gcacgagttc ctgcccacca aagaggagga catcaaggag atcgccgggg agctggggat
      661 caccgtcgaa aagctgcaca acgtcatcga ctcgctgcat gagttcaacc cgatgatggg
      721 ccaccgcggc tgccgtctgg ccgtctccta cccggagatc gcggtcatgc agacccgcgc
      781 cgtcatcaac gcggctctca atgtgtccaa gaaaaagggc atcaagatcg tgccgcacat
      841 catgatcccg ctggtgggcg aggtcaaaga gctcaagttc gtcaaggacg tggtggtcaa
      901 gaccgctgat gagatcatcg ccgccgccgg tgtggagatg aagtatgagg tcggcaccat
      961 gatcgagatc ccgcgtgccg ccctgaccgc ggacgagatc gccaaggagg ccgagttctt
     1021 cagcttcggc accaacgacc tgacccagat gacgttcggc ttcagccgcg acgacgccgg
     1081 caagttcctc gactactact acgagaacaa gatctacgag ctggatccgt tcgcgcacct
     1141 ggaccagaac ggcgtgggca agctggttga gatggctgcc aagctgggcc gtcagacccg
     1201 tcccgacctg ggcctgggca tctgcggcga gcacggcggc gacccgacga gcgtcgagtt
     1261 ctgccacaag gtcggcctgg actatgtgtc ctgctcgccg ttccgcgtgc cgattgcccg
     1321 ccttgccgct gcgcaggctg cgatcaagtt tccccgctga gaacgattga aagttgaaag
     1381 ccggacggat tgcccggtga gataaaaaac acgcacatcg ctgactgcga tgtgcgtgtt
     1441 tttttgccgc cttttttgcg tctggttttc gcctggcttt tttggggggg acgcagtgct
     1501 tttccattca ccctcaaaat gatctttctc agaagaaaaa aggagctctt ctataaaaga
     1561 ggatggaaag ggtattaaag cacggttttc cagaagccgt gcaggttgca gtaggcatag
     1621 gcagccaaag gccggtcgcc cggggcgagg gcgaaaacgg ccaccggctc tccctcacag
     1681 cacagatcta cccgctggca gcctttttcc gtctgcagat agacccattg gatgctgtgc
     1741 tcggcggaca tggggtgaaa gacgctgccc accttgacgg tgaccatgtc cccactgcgc
     1801 tcgaccaccg gcaaatgctt ttcctgagcg ccctccgagg tgttgggcgt cagttcctcc
     1861 aattccttgc cgccgcagga atagtgggtc ttgttgccgg cgatctcttc gatgaggacg
     1921 ccgcattttg tgcaggcgaa aaatctgggt tccttgtcag gcatttgaac tccctcctgt
     1981 tttttgtgaa ttgtcgtttg ttgagaccgg cggccgggaa aacaggacat ggccgcgcgg
     2041 ccgaggctgt gcggcggaac gccacggata ggtatcccgc ccgggtggga accctaaagg
     2101 ggcccgcctt tcccctgccg ggcgtttcgg ccgacggcag tcccggccgg gcatctccgg
     2161 ccatgcgttc tgccgcccgg cggctctccc tgcaccgatc tcgcgataac ctatagtgtt
     2221 tcccaaaaag cgggcgccat tcaaaaagca caaggtgcct tgcggccggt gcgggtccac
     2281 gcagggcaga aaaagagaaa ggcggcgcca caaaaggcgc agggatggaa aaggctgccg
     2341 gtgctgcccg tccggcgaaa ggtgaagagc gtcggcgggt tgcggaaaag gcttggatgc
     2401 agggaaacag cgcgcggcaa gcttggggcc agcgaacggc gcgggcagat cagacaggtc
     2461 tgtcggtcag aaccagttga tatttacttc ctgctggaac tgcgcaaaag ggcgaaaaag
     2521 catcggcaag cggaaagaga gcgggaaaaa cgggtggcca gaggcggaaa aaaccgcaaa
     2581 acaaaaggaa aggcagaaga agataaatat gaattattat tgcatgatga tagttttggc
     2641 ttgaaagcaa ttgactattt aactagaaaa aatccaaaat aaaatcttaa aatagagatt
     2701 aaatacaaaa atattgcggc aaatctgcta ttattttgcg tatccagaag atgaagaaag
     2761 agtaacaagc ccatgtaaga atgttgcaat tttttttaca cacttctcac acaggcggcc
     2821 ggtatagtgg gtgacacgag gtgataaaaa atgaaaaaga ttatgacgat tgctcttgct
     2881 cttaccctgg cgctgggcct ggtcgcctgc ggcagcgata gctcttcttc caccccggct
     2941 tcttccagcg cggtgtcctc ctctgtctct gcccccagca gtgtggcgga ttccagcgag
     3001 gccggctcca gcgaggcgga ttcgaaagac acgagctctt ccgcggctga ttcttctgag
     3061 gccagctcct ccgaggccgg ctccagcagt gcggcttcgt ccagcaaggt cgaagagtaa
     3121 gcagacggcg tttgccgttt taaaaggacg cccctctctt atgagagggg cgtccttttg
     3181 tgcttaaaaa gtaacaaacg gcatgaatgg gatgatttgt aacccttgct tgataccctt
     3241 tgccaaaggt ggtataatga gatactgaat aattttgtaa ccgactggta aattttggtg
     3301 gactgtttta ctgagaagaa cagggccggt tgtttttgag aggggaggag gcgcgcagtg
     3361 aaacgaaaga gcgggagggt gttggcttca ctgctgatgg cgtttctttt tctcttttcg
     3421 gcggttcccg cacagggggc aggggagacc ggccgcacga tccgggttgc gtatccctac
     3481 caggcgggcc tcaccgaggt ggatgaacag ggcaattact ccggctatac ctatgaatat
     3541 ctacaggaga ttgcgcagta taccggctgg agctatgagt ttgtagaggt gcccggcacc
     3601 ccggatgaga gcctgaccca gctgatggag atgctggaga acggcgaggt cgacctgatg
     3661 ggcggcatgc tgtacagcgc cagcatggcg gagctgtatg attattccgg ctacagctac
     3721 ggcgtggtgg agaccgtgct gcaggtgctg caggaggata cccgcgacct ggtggtggat
     3781 tcgcagacgc agcagacttt tcgggtggcg gtgcccagca gcagcaaaca gcggttgaaa
     3841 gagctgcagg agtactgcga tatgaacctg atcgagctgg ttccggtgct ctgtgcggac
     3901 aaggcggagc agctgcaggc cctgcgcgag ggcagggcgg acgcgctgct ggccaccagc
     3961 atggattaca cgcccgggct gaggaccatc gcccggtttg cacagaagcc gttttatttt
     4021 gtgctctcta aaaaatgcga tcagggcatc atgcaggagc tgaacgccgc catcgcggac
     4081 gtggagcgcg cggaccccac ctattccacc gcgctgtttg aaaagtattt tatgcccaaa
     4141 aacgacaccg ccgtgctcac cgacgccgaa caggcctatt tggccgccgc gggtaccctg
     4201 cgggtggggg tcatggacga ccagccgcct ttccagtacc gggaggaggg ggcttttgcg
     4261 ggcattgcgg tcgacctgct ggaggtcatc gcccaggaga cggggctgca gtttgagctg
     4321 gtgccggtaa agggcactgc ggcgctggaa gagctgctcg aggccggaga gctcgacctt
     4381 gtggcgggga tgacctatga ctacgatgag gccaaccggc tgggtctggc catgtcccgc
     4441 ccttacctgt cggcgcagta tgtgcagctg cagaagacct cttcccccga gagcagcggc
     4501 gggcggctgg ccgcgtccgt aaacaacaac tacggcagcg ccgcggcgag cgaggtgctg
     4561 tggtacgaca gcatcagtga atgcgtcgcg gcggtggacc ggggcgaggc ggattttacc
     4621 tttgtggacg cctataccgg gcaatattat gtcaacacct ccgcctacgc ggacctgaaa
     4681 gtggtctctc agctgtcggg gcagcgccag atctgttttg gcctggtcaa gcccaccagc
     4741 cgtcagctgc tgagcgtcct caacaaggag ctggcggtgc tgcccgcggc ccacatgcag
     4801 gagatcgtct acgtcaacac cctctatacg cccgagatca gcctgcgcga actgctgcgg
     4861 gatcaccccg cagtggcggt cagcctggtg gccggggtcc ttttggcggt gatcgcggtg
     4921 ctgctgatct ttttgcggca gcgcaatgcc tccagccgcc gtctggccct ggagctgaaa
     4981 aagcatctgc aggtctacgg catggtgagc gacgcgtttt ttgagtacga ctatgccgcc
     5041 cagcggctca tcatctccac cccgcatgcg ggtgaggatg gagaggcgga gctgaagagc
     5101 tatttcctgg gcggtacgga ggagatagac ccctttattg gctcctttgt ggattttgtg
     5161 cgtcagagcg aggacggcat gcacgaaaag cagttgccgc gcgataatgg gcagctgcac
     5221 tggctgcggg tggcgacccg caccatccat gacgatcatg gcaagcgggc ctataccgta
     5281 gggcgcatca gcgacatcga agaggagcgc agggcccagc aggagctgtt ggaaaaagcc
     5341 cagcgggaca gcctcaccgg cctgttcaac accgccgccg gcaggcagcg cagcgaggag
     5401 cttctggccg gcctgcgggg cgggaaccgg gacgcgctga tcctgctgga catcgactat
     5461 ttcaaagagg tcaacgaccg gtacgggcac cgccgcggcg actgggtgct cactcgtgtg
     5521 gcggaggtgc tgcgcagcag cttccggccg gaggacgtga tcggccgtcc cggcggcgac
     5581 gagttcgtcg tctatctgcg gggcgcgcgg gagctggccg cactgggaga gcgctgtggg
     5641 accctttgcc gccggatccg cgagctgagc cgggacggcc tgcacctgtc ggtcagtatc
     5701 ggggcggcgc tggcgggccc cggagacagc tatgaagccg tgtatgagcg ggccgaccag
     5761 gcgctgtacc gggccaagga cgcgggccgc gaccgctata tccttgtgcc ttggccgggg
     5821 gatacgcagg gggtggccgg ggacgaggcc ctgcggggat gagcagcttt cgtattctga
     5881 gagaaatcaa aaaagacgag cgatctttga tcgctcgtct ttttttgcgc ggtggatcag
     5941 gacaaaagcg ccgcaaaagc agcgggcgcc agcgggcggg agaaatagta gccctggatc
     6001 aggtcgcagc cggcggcctg caaaaagtcc agctgcggct tctgctccac cccctcggcc
     6061 ac
//
LOCUS       DBMDYA010000076         6055 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3315_length_6055_cov_1.587840, whole genome shotgun sequence.
ACCESSION   DBMDYA010000076 DBMDYA010000000
VERSION     DBMDYA010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_6055_cov_1.587840"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="ACZPFK_02940"
     CDS             <1..490
                     /locus_tag="ACZPFK_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1405300.1"
                     /translation="VVLCQGSYEHTTDKYDYQGVKSCAASAALYGGSSACPYGCLGYG
                     DCVKACKFDAIHVENGLSRVDPDKCTGCGECAKACPKHIISVRPVSANPIVLCANEDR
                     GALTHKECTAGCIGCMKCTKVCPEEAITVKDNRARIDPAKCISCGACVLTCPVGAIVS
                     FG"
     gene            complement(618..1505)
                     /locus_tag="ACZPFK_02945"
     CDS             complement(618..1505)
                     /locus_tag="ACZPFK_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1405301.1"
                     /translation="MDLRTRALPRLLNREVEAYLQENDIILVPVGAVEMHGDLPLDCE
                     TVLSEAAALKMAEACDGLVLPGLPYFYAGATAIGRGTTQVSVRQGIDYLGAVARSLLR
                     QGFRRQIYVSLHGPAYLTCSPMVRDFFDETGVPILYVDLLLQLERLGKDLFPPEQPPY
                     RRLDALILGAYDLLGRLEEVPLSPGGGAALSPRGEAAPPPAPTTAPFEQLFGLAYQSG
                     GAGYCFGAASDHMPTTPLPTPEARRALAEEGKAALCALVRRLELPRMVRQLAELARFE
                     AQVGTRFPWMPGARGMRGE"
     gene            1746..2399
                     /locus_tag="ACZPFK_02950"
     CDS             1746..2399
                     /locus_tag="ACZPFK_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1405302.1"
                     /translation="MASILIAEDEKPIRELIRRNLLLVGHSCREAADGREALELAEAE
                     RFDLALLDVMLPGTDGFEVKRRLPQELPVIFVTARTGLSSRLQGLGLGADDYILKPFE
                     ILELLARVEAVLRRTGAGDREVEAGGCRIDLAQRRVTREGAEIPLKPQEYALLEVLVR
                     NRNLALSREKLLELAWGYDYEGDTRTVDVHVQRLRKKLGWEKELQTVYKLGYRLSTK"
     gene            2410..3963
                     /locus_tag="ACZPFK_02955"
     CDS             2410..3963
                     /locus_tag="ACZPFK_02955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405303.1"
                     /translation="MKRAQGAAGGTQKREKSGAGARPGGRRLARAALARLSFWQKIYL
                     CALALFLACLNLGVFAVAAVGQSSSFAAERDRLLTRQHFIAEMLAQDMAAVEARRPGA
                     LPALAEGYAQSYGGSDTWLKVVRGEQTLADRLPAPDGGAVLPHAAEEGTRVWNVLPSG
                     GRRLLYVSALLPGQGERTALTCAFDMEGFLAGWEATARVFQAIALAASAVLAAGLYFT
                     LRGLGRPLARLAQAARAIGDGDYTARTREEGRDEIGQLAHAFNEMARRTGETVEGLAE
                     AAREKQRLADNLAHELRTPLTAIGGYAEYIARAELSGEEKAEAAGYIMEETRRLASMS
                     ERLLAMASLREGGLCPAPLDLAEAARAALRAVRPKAAAQGVALEPRLPAHLPFWGGRD
                     LLVSLMVNLLDNGVKASRRGGRVTVSLAREKKEALLTVEDEGVGMSAEPLARLGEPFY
                     RADRSRSREQGGTGLGVAFCFAVARAHGAVLTFESQPGRGTRAQVRFPTGERLISAPA
                     PAEKRKAPG"
     gene            4220..5209
                     /locus_tag="ACZPFK_02960"
     CDS             4220..5209
                     /locus_tag="ACZPFK_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405304.1"
                     /translation="MKEKMREFVKGRAAALLTVGVAVLVIAAGAMILHGCMGWGKEDF
                     SGGDSDSLSVVPAADAAMGVAENGKLYDYFWRGEMDEQQLEEQCEKEWQKILAEYQEM
                     DADQETIENRRKELDGEIDWMKKELEKARLLDPAVSAQEAANTAGMLFEDAYGLYPEE
                     NTLFLDCVENPDSRGVKRLEWLIQTGRQNPSEEGMGFCAVDATTGKVISCSYLPSGWE
                     TENCVSEELLPVVRTYSEEGRRLYAYDPSHPQWPEFAEEKRAQIQAFLRQAGLDKNRT
                     VERIQAEENELNGFRFTVSFADGGKAEIQFSLRPWCVSDWGEYPGKGMEYHTL"
     gene            5332..>6055
                     /locus_tag="ACZPFK_02965"
     CDS             5332..>6055
                     /locus_tag="ACZPFK_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405305.1"
                     /translation="MLRITVLSENTAVRPGLDTEDGLSVFIETDRHKILMDAGWNDVF
                     LNNAARLGAPVEEADAAVLSHDHYDHGQSFPLFFERMAGRKYLFYLSSDVLWSGYHRR
                     PEGALTSTGCGLSDAELIRARVPHRYVEKSVFYPFADEPGVALLSRFEKRCNFEPMLD
                     EFVKLRGGELVRDEFDHELAMALPVREGLVLLTGCAHSGICNMILAAEERLGRPVVAV
                     LGGTHLKASGPERAMATIDFFNG"
BASE COUNT         1098 a         1798 c         2213 g          946 t
ORIGIN      
        1 cgtcgtgctc tgccagggct cgtatgagca caccaccgac aagtacgact accagggcgt
       61 caagagctgc gccgcctccg ccgcgctcta cggcggctcg tccgcctgcc cctacggctg
      121 cctgggctac ggcgactgcg tcaaggcctg caagttcgac gccatccatg tggaaaacgg
      181 cctctcccgc gtggacccgg acaagtgcac cggctgcggc gagtgcgcca aggcctgccc
      241 caagcacatc atctcggtgc gcccggtcag cgccaatccc atcgtgctct gcgccaacga
      301 ggaccgcggc gccctgaccc acaaggagtg caccgcgggc tgtatcggct gcatgaagtg
      361 caccaaggtc tgcccggagg aggctatcac ggtcaaggac aaccgggccc gcatcgaccc
      421 tgccaagtgc atcagctgcg gcgcctgcgt gctcacctgt ccggtggggg ccatcgtgtc
      481 tttcggctga gcctttggcg cggccgcgcg gcgcgggccg tgacgggccc ggcgaaaggc
      541 cgaaccgatc aacctgaaaa aggactgtcc caaaagcgcg ttgatacgct tttgaggcag
      601 cccttttttg ttttttctca ctcgccccgc atgccacggg cccccggcat ccaggggaag
      661 cgcgtcccca cctgcgcctc aaagcgcgcc aattccgcca gctgccgtac catgcgcggc
      721 agctccagcc tgcgcaccag cgcgcagagc gccgccttgc cctcctcggc cagggcgcgg
      781 cgcgcctcgg gggtgggcag gggggtggtg ggcatgtggt cgcttgcagc gccaaagcag
      841 tacccggcgc cgccgctctg ataggccagg ccgaacagct gctcaaacgg ggccgtcgtc
      901 ggggcgggcg ggggcgccgc ctcgcccctg ggcgagagcg ctgccccgcc cccgggcgag
      961 aggggaacct cctccagccg ccccagcagg tcgtaagcgc ccaggatcag ggcgtcgaga
     1021 cgccggtagg gcggctgctc agggggaaac aggtccttgc ccagccgctc cagctgcagc
     1081 agcaggtcca cgtagaggat gggtaccccc gtctcgtcga aaaagtcccg caccatgggg
     1141 ctgcaggtca ggtaggcggg cccgtgcagg ctgacgtaga tctggcggcg aaagccctgg
     1201 cgcagcaggc tgcgggcgac cgcgcccaga tagtcgatgc cctggcggac gctcacctgg
     1261 gtggtgccgc ggccgatggc ggtggcgccc gcgtaaaaat agggcaggcc gggcagcacc
     1321 aggccgtcgc aggcctctgc catcttcagc gccgccgcct cgctgagcac ggtctcgcag
     1381 tccagcggca ggtccccgtg catctccacg gcacccaccg gcacgaggat gatgtcgttc
     1441 tcttgcagat aggcctccac ctcacggttg agcaggcggg gcagggctct tgtcctcaga
     1501 tccatggcgc gctccttttt caagacggat tttgacgcgg gaaagggtac acttaaagac
     1561 taagggctgg cgacagggat gtaaattgca aatctggcag aaaagatgat atatccggca
     1621 aaaaagggag atggcggccc cgttacaact tggcaatcgg ccggcgctat gctggcggtg
     1681 gtacaatgga aaaaacagac ccgcccgggg gcaacccgcc cgggggggga gaggaggaaa
     1741 ggcagatggc atctatcctg atcgcggagg acgaaaaacc catccgggag ctgatccggc
     1801 gcaatctgct gctggtgggg cacagctgcc gggaggcagc ggacgggcgg gaggcgttgg
     1861 agctggcgga ggcggagcgc ttcgacctcg ccctgctgga cgtgatgctg ccgggcaccg
     1921 acggctttga ggtcaagcgg cggctgccgc aggagctgcc ggtgatcttt gtcaccgcca
     1981 gaaccgggct ctccagccgc ctgcaggggc tgggcctcgg ggccgacgat tatatcctga
     2041 agccgtttga gatattggag ctgctggcgc gggtggaggc ggtgctgcgc cgcaccggtg
     2101 ccggggaccg ggaggtggag gcgggcggct gccgcatcga cctggcccag cgccgggtga
     2161 cgagggaggg ggcggagatc ccgctcaagc cgcaggagta cgccctgctg gaggtgctgg
     2221 tgcgcaaccg caacctggcc ctcagccggg aaaagctgct ggagctggcc tggggctatg
     2281 actatgaggg agacacccgc accgtggacg tacatgtgca gcggctgcga aagaagctgg
     2341 gatgggaaaa ggagctgcag accgtgtaca agctggggta ccggctgagc acaaagtgag
     2401 gggaaagaca tgaagagagc acagggggca gccggcggga cgcagaagag agaaaaaagc
     2461 ggcgctggcg cgcggccggg gggccgccgc ctcgcccgcg ccgcgctggc gcgcctctct
     2521 ttttggcaga agatctatct ctgcgcgctg gcgctttttt tggcctgcct caatctgggc
     2581 gttttcgcgg tggcggcggt tgggcagagc agcagctttg cggcggagcg ggacagattg
     2641 ctgacccgcc agcacttcat cgccgagatg ctggcccagg acatggcggc cgtggaggcg
     2701 cgcaggcccg gcgccctgcc ggcgctggcg gagggctatg cgcagagcta cggcggctcg
     2761 gatacctggc tgaaagtcgt tcggggagag caaacgctcg ccgaccggct gcccgcgccc
     2821 gacggcggcg ccgtcctgcc ccacgcggcg gaggagggga cgcgggtctg gaacgtgctg
     2881 ccctcgggcg ggcgcaggct gctgtatgtg tcggccctgc tgcccggcca gggggagagg
     2941 acggccctga cctgcgcctt tgacatggag ggctttttgg ccgggtggga ggccacggcc
     3001 cgggtttttc aggccatcgc cctcgccgcg tcggcggtgc tggcggccgg cctgtatttc
     3061 acgctgaggg gccttggccg cccgctggcg cggctggcgc aggcggcgag agcgatcggg
     3121 gacggggatt acaccgcgcg cacccgggag gagggccggg acgagatcgg gcagctggcc
     3181 catgcgttca acgagatggc gcgccgcacc ggcgagacgg tggaggggct cgccgaggcg
     3241 gcaagggaaa aacagcgcct tgccgacaac ctggcccacg agctgcgcac cccgctcacc
     3301 gccatcggcg gctatgccga gtatatcgcc cgggccgagc tttcggggga ggaaaaggcc
     3361 gaggcggcgg gctatatcat ggaggagacc cggcggttgg cctccatgag cgagcggctg
     3421 ctggccatgg cctcgctgcg ggagggcggt ctgtgccccg cgccgctgga tttggcggag
     3481 gcggcccgcg cggccctgag agcggtgcgc cccaaggcgg cggcgcaggg ggtggcgctg
     3541 gaaccgcgcc tgcccgcgca tctgccgttt tgggggggcc gggacctgct ggtgagcctg
     3601 atggtgaatc tgctggacaa cggggtgaaa gccagccgcc ggggcggccg ggtgacggtg
     3661 tcccttgcgc gggagaaaaa agaggcgctg ctcaccgtag aggacgaggg cgtggggatg
     3721 agcgcggagc ccctggcgcg gctgggagag cccttttacc gggccgacag atcccgcagc
     3781 agggagcagg ggggcaccgg gctgggggtg gctttttgct ttgccgtggc gcgggcccac
     3841 ggggcggttt tgacctttga gtctcagccg ggccgcggga cccgggcgca ggtgcgtttt
     3901 cccacggggg agcggctgat ttccgccccg gcgcccgcag aaaaaaggaa agcgccgggg
     3961 tgaaacggcc cctcccccgc gaggccgcgg atgaaaaagc cgcacgggtc cgtcgagctg
     4021 tgagatcaca ggctgccggc ggcggacagc ccccgcccta tggcccgggc gcgctgcttc
     4081 gcgggcaccg ctgcgggcct ttgcgggggg ggctgcaaga tgcgcgctcc ggcggatcgt
     4141 tacagcttgg agataacttt gcagctttgc agcaataccg gcagtggata ctggaggcat
     4201 ggaaaggagg cgcccgaaca tgaaagaaaa gatgcgggaa tttgtaaaag gacgcgccgc
     4261 ggcgctgctg acggtggggg tggcggttct cgtgatcgcc gccggggcga tgatcctgca
     4321 cggctgtatg ggatggggaa aagaagattt ctccggtggg gacagcgact cgctgagcgt
     4381 tgtcccggcc gcggacgcgg cgatgggggt cgcggagaac ggaaagctct atgattattt
     4441 ttggagggga gagatggacg aacagcagct ggaggagcaa tgtgaaaaag agtggcagaa
     4501 aatactggcg gagtatcaag aaatggatgc ggatcaggaa accatagaaa atcggcggaa
     4561 agagcttgac ggggagatcg attggatgaa aaaagaactg gaaaaggccc ggctgcttga
     4621 cccggccgtc tcggcgcagg aggcggccaa caccgcgggg atgctgtttg aagacgccta
     4681 tgggctgtat ccggaggaaa acacactttt tctcgactgt gtggaaaacc cggacagccg
     4741 cggggtaaag cgtctggaat ggctgataca gacgggcagg caaaacccca gcgaggaggg
     4801 gatgggcttc tgtgccgtcg atgcgacgac gggaaaggtg atcagctgca gctacctgcc
     4861 ctccggctgg gagaccgaaa actgcgtcag cgaagaactt ctgccggtgg tcaggactta
     4921 cagcgaggag ggcaggcggc tctacgccta tgatccgtcc cacccgcagt ggcctgaatt
     4981 cgcggaggaa aagcgggcgc agatacaagc gttcctgcgc caggcgggcc tcgacaaaaa
     5041 caggacggtt gaaaggatcc aggcggaaga aaatgaatta aacgggtttc ggttcaccgt
     5101 gagctttgcg gacggcggaa aagcggagat acaattttca ctgcggccct ggtgtgtcag
     5161 tgattggggg gaatatccgg gaaagggaat ggagtaccac acgctgtgaa aaaggcggaa
     5221 cggccgcggg cctgaaagct tgcccaagcg gagctttccg cacaaaaacg gaaccggggc
     5281 gacgcgtttt gtgctatact gttaaaaaaa gtgcaagggg aggttttcat catgctgcgc
     5341 atcaccgtcc tgtctgaaaa caccgccgtg cggcccggtc tcgataccga ggacggcctg
     5401 tcggtcttta tcgagacgga tcgccacaaa attttgatgg acgcggggtg gaacgacgtc
     5461 ttcctcaaca acgccgccag gctgggggcc ccggtggagg aggcggacgc cgccgtgctc
     5521 tcccatgacc actatgacca cgggcaatcc tttccgctgt tttttgagcg catggcaggg
     5581 cgcaaatatc tgttctatct ctccagcgac gtgctgtggt cgggctatca ccgccgcccg
     5641 gagggcgcgc tgacctccac cggctgcggc ctgtcggacg cggagctgat ccgcgcgcgg
     5701 gtgccccacc gatatgtgga aaaaagcgtt ttttatccct ttgcagacga gccgggggtg
     5761 gcgctgctct cccgttttga aaagcgctgc aattttgagc cgatgctgga cgagtttgtc
     5821 aagctgcggg gcggcgagct ggtgcgggat gagttcgacc acgagctggc catggccctg
     5881 ccggtgcggg agggcctggt gctgctcacc ggctgtgccc acagcggcat ctgcaacatg
     5941 atactggcgg cggaggagcg gctgggccgc ccggtggtcg ccgttttggg cggcacccac
     6001 ctaaaggcca gcggccccga gcgggccatg gccaccatcg attttttcaa tggac
//
LOCUS       DBMDYA010000077         6040 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3322_length_6040_cov_1.710052, whole genome shotgun sequence.
ACCESSION   DBMDYA010000077 DBMDYA010000000
VERSION     DBMDYA010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_6040_cov_1.710052"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="ACZPFK_02970"
     CDS             <1..489
                     /locus_tag="ACZPFK_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405306.1"
                     /translation="YPLDLDDFDLIDMVRIGENTVVTTSSDAQMLLKSVPEECVSLKY
                     RAEYSNDSGAIEIFYSRGQEDFSIRRRLWPSRVAGDTFVCALPRGTIGRLRLDPTLLA
                     GVTITFSEIVLNAPRSFFSYFAVSRTQVFWFLILPALAASALQWLADLIQFIAAKKGM
                     RS"
     gene            489..1649
                     /locus_tag="ACZPFK_02975"
     CDS             489..1649
                     /locus_tag="ACZPFK_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HIR1405307.1"
                     /translation="MTEKFIPLSVPNFSGNEKEYVNGAVVSEWVSTGGSLVGEFEQKV
                     TGYAGMPAAVACNSGTSGLHLANLVAGVGRGDEVLAPALTFIAAVNPIRYVGAEPVFI
                     GCDDSLCMDPALAERFLAENCEMRGGCCYNKRTGARVRAVVVVHVFGNMADMPAFLAL
                     AKKYNLVLIEDATEALGTRYTEGPLAGRMAGTMGDVGVYSFNGNKIITTGAGGMVVSN
                     HSDWAEHAKYLSTQAKDDQLQFIHGEVGYNYRLTNVQAAMGLAQLEQLEGFIEHKIAM
                     HAFYTEKLDGKNGYRVLGFRDGVRANHWFYSLYLEDPRHQRDAVIAALSEQKIQTRPV
                     WALINEQKPYLQCEAHGLAKAEDYRARIVNLPCSTNLTEEDARRVVDALLAL"
     gene            1688..2737
                     /locus_tag="ACZPFK_02980"
     CDS             1688..2737
                     /locus_tag="ACZPFK_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1405308.1"
                     /translation="MKLDELLIEEDRTVLEAMRRLDETGHRILFVAPQGKLKAVVTDS
                     DVRRYILRGGALTGPVREMANYHPKSVAVEERGRARKLLLQYSIDALPVLDRRGVIND
                     VIFEGDLDVDTHKKADLPVVIMAGGLGTRLYPYTKILPKPLIPVGELPIAEHIINRFK
                     NFGCYEFTLVVNYKKSMIKSYFNDLEKDYVVRYADEDQPLGTGGGLALLKGKVTSTFF
                     LTNCDVLIDADFGDIYDFHRRQGNLITVVCAFKHITIPYGVIELGGEGEIKNVTEKPE
                     MNFLTNTGVYVVEPRVIDELEDGVAVGFPDIMEKYRHAGEKIGVYPVSENSWMDMGQL
                     EELENMRRRLEEQNG"
     gene            2759..5323
                     /locus_tag="ACZPFK_02985"
     CDS             2759..5323
                     /locus_tag="ACZPFK_02985"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1405309.1"
                     /translation="MQDTHRNAKNVRYTLLLSAVMVLMLYLLLGIYPFGDGTVLTGDL
                     NGQYISYFAHFRRALLSGEGFAYGFDKALGGGLLGIFAYYASSPFNLLYLFFEPAAYC
                     DVAALLLALKLVAAGGCMTFFLDRHRGGCGRWSIPLGLGYSFMTYAVVYAQNIMWHDV
                     VLLLPLVCHGIDRILSRRSPLVFTLTLAAGIWANFYIAYMVCLFSVFYFGYEMLLERK
                     EKGTRAAFWKSRCLGFASGALTAGLLNGILLLPALENIQATKGELLEYSFSWQQTFSL
                     ARLPAQLLPGGFVWQDVVDGLPNLYCGLLAVICAIGYFCSKKISGREKLLSGAFLGGL
                     VFTMWIRGLDNLWHGLKRPVWFPYRESFLLSFLILFLAAGALRRASFSRKKAVLFLVL
                     NAALLFLALLFRSPAMGGRRAPLAALLWCMVIGLVAAALWGPHRLQKIAFLGCVLVTA
                     GELTLNAYWSADQFEKYSRSDYRTFVERGTAVVDTLGAGAGSNFRMEKNFFRSLNDPM
                     LLGYHGTAHFGSTQDVANTQMLNSLGYRSYGGSNSYGCGSTAFADAFLGIRYLLSDGA
                     FPVGSHYEDMGEKEGWEIEQNPYAFPIGILLEKLDPIEAEEGSGVSTFEFQNRLYALL
                     SGEKDPLFTPVETLSAFCGGEPASVTGTFEEEMEYRFTAPDDGLYYAVMTSGSGLPLS
                     LSVEGKLVESGYFTAEQSGVIDLGRREAGESCTFAVAPRQESASVEEIAIYRMDENSL
                     RALSERLNRENEGLTLKEGTAHGIVEAKQDGWFLMQIPYEEALVISVDGKETQAVEAG
                     GSCVAVALNAGKHELDVAYRAPGVTAGVVLSFAGAAGLLGLIAWTIKIDKKEQREG"
     gene            5326..>6040
                     /locus_tag="ACZPFK_02990"
     CDS             5326..>6040
                     /locus_tag="ACZPFK_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR1405310.1"
                     /translation="MPVCIIAEAGVNHNGDLALAKKMAVVAKNAGADIVKYQTAVPEL
                     VISKFAEKAEYQKVQTGDHETQLEMVRRIHFGFEAHRELKAYCDSIGIQYLSAAFDLP
                     SIDFLASLDPPLFKIPSGEITNLPYLERVAACHKPVLLSTGMSEMEEIAAALNALKLG
                     GAGEVTVLHCNTEYPTPAEDANLRAMLDIQKRFGCRVGYSDHTLGIEADIAAVALGAQ
                     VVEKHFTLDKNMEGPDHKAS"
BASE COUNT         1197 a         1645 c         1942 g         1256 t
ORIGIN      
        1 tacccccttg acctggacga ttttgacttg atcgatatgg tgcgcatagg ggagaacacg
       61 gtggtcacca caagctctga tgcgcaaatg ctcttgaaaa gcgtgcccga ggagtgcgtt
      121 tctttaaaat atcgcgccga gtacagcaat gactcgggag caatagaaat tttttacagc
      181 cgagggcaag aagatttttc aataaggcgc aggctctggc ccagccgagt cgcgggggac
      241 acttttgtct gcgcgctccc acgggggaca attgggcgcc tgcgcctgga ccccaccctg
      301 cttgccgggg tgacaattac cttttctgag atcgttttga atgcgccccg cagctttttc
      361 agctattttg cggtgtcccg cactcaagtg ttttggttct tgatcctgcc ggccctcgca
      421 gcctccgcac tgcaatggct ggccgatttg atccagttta tagcggcgaa gaaaggaatg
      481 agatcatgat gacagaaaaa ttcatacccc tgtccgtgcc caacttttcg ggcaacgaaa
      541 aagagtacgt caacggcgcg gtcgtcagcg agtgggtctc caccggcggc agcctggtgg
      601 gcgagtttga gcaaaaggtg acggggtacg caggcatgcc cgccgcggtg gcctgcaaca
      661 gcggcacctc cggcctgcat ctggccaatc tggtggcggg tgtgggccgc ggggacgagg
      721 tgctggcccc ggcgctcacc tttatcgcgg cggtgaaccc catccgctat gtgggggcag
      781 agccggtgtt catcggctgt gacgactcgc tgtgtatgga cccggccctg gcggaaaggt
      841 ttctcgctga gaactgtgag atgcgcggcg gctgctgcta caacaagcgc accggcgcgc
      901 gggtgcgggc cgtcgtggtg gtgcacgtct tcggcaacat ggccgacatg cccgcctttt
      961 tggcgctggc aaaaaaatac aacctggtgc tcatcgagga cgcgaccgag gcgctgggca
     1021 cccgctacac cgagggcccg ctggcgggcc gcatggccgg caccatgggc gacgtgggcg
     1081 tctacagctt caacggcaac aagatcatca ccaccggcgc cggcggcatg gtggtctcga
     1141 accactccga ctgggccgag cacgcaaaat acctctccac ccaggccaag gacgaccagc
     1201 tgcagttcat ccacggcgag gtgggctaca actaccgtct gaccaacgtg caggcggcca
     1261 tgggcctggc ccagctggag cagctggagg gctttatcga gcacaagatc gccatgcatg
     1321 cgttttacac cgaaaagctg gacggcaaaa acggctaccg ggtgctgggc ttccgggacg
     1381 gggtgcgggc caaccattgg ttctacagcc tgtatctgga ggacccgcgc caccagcggg
     1441 acgcggtcat cgcggcgctg tcggagcaga agatccaaac ccggccggtc tgggcgctca
     1501 tcaacgagca aaagccctat ctgcagtgcg aggcgcacgg gctggcaaag gccgaggact
     1561 accgcgccag aatcgtcaac ctgccctgca gcaccaacct gaccgaagag gacgcccggc
     1621 gggtggtgga tgcgctgctg gcgctgtgag gcccggcgca ggggataaaa agggggagtg
     1681 agcccggatg aagctggatg aactgctgat cgaggaggac cgcacggtgc tggaggccat
     1741 gcggcggctg gacgagaccg gccaccgcat cctgttcgtg gcgccgcagg gcaagctgaa
     1801 agcggtggtg accgacagcg acgtgcgccg ctacatcctg cggggcggcg ccctcaccgg
     1861 cccggtgcgg gagatggcga actaccaccc caagtcggtc gcggtggagg agcggggcag
     1921 ggcgcgaaag ctgctgctgc aatactccat cgacgcgctg ccggtgctgg accggcgcgg
     1981 ggtcatcaac gacgtgatct ttgagggcga cctggacgtg gacacccaca aaaaggcgga
     2041 cctgccggtg gtcatcatgg ccgggggcct gggcacccgg ctgtacccgt ataccaagat
     2101 cctgcccaag cccctcatcc cggtggggga gctgcccatc gccgagcaca tcatcaaccg
     2161 gttcaaaaat ttcggctgct atgagttcac gctggtggtc aactacaaaa agagcatgat
     2221 caagagctat ttcaacgatc tggaaaagga ttacgtcgtc cgctacgccg acgaggatca
     2281 gccgctgggc acggggggcg ggctggcgct gctcaagggc aaggtcacca gcaccttttt
     2341 cctcaccaac tgcgacgtgc tcatcgacgc ggactttggc gatatctatg acttccaccg
     2401 ccggcagggc aacctgatca cggtggtctg cgcgttcaag cacatcacca tcccctacgg
     2461 ggtgatcgag ctgggcggcg agggcgagat caaaaacgtc accgaaaagc cggagatgaa
     2521 ttttttgacc aacaccggcg tctatgtggt ggagccccgg gtcatcgacg agctggagga
     2581 cggggtggcg gtgggctttc ccgacatcat ggaaaaatac cgccatgcgg gcgagaagat
     2641 cggggtctac ccggtgagcg agaacagctg gatggatatg ggccagctgg aggagctgga
     2701 gaacatgcgc cgccgcctgg aggagcagaa cggctgaggc cgtctgcggg agaataggat
     2761 gcaagataca catcggaatg ccaaaaatgt acgctatacc ctgctgcttt cagccgtgat
     2821 ggtgctgatg ctttacctgc ttttggggat ttatcctttt ggggacggca ccgtgctgac
     2881 cggtgatctc aatggacagt atatcagtta tttcgctcat tttcgacggg cgcttctctc
     2941 gggtgagggc tttgcctatg gattcgacaa agcgctgggg ggagggctgc tggggatatt
     3001 tgcttattat gcatccagcc ctttcaacct gctctatctt ttttttgaac cggctgccta
     3061 ctgtgacgtc gccgcgctgc ttctggccct caagctggtt gcggcgggcg ggtgcatgac
     3121 cttttttctc gatcgccaca ggggcggctg cggcagatgg agcatcccgc tgggccttgg
     3181 ctacagcttt atgacctatg cggtggtcta cgcccaaaat attatgtggc atgatgtggt
     3241 actgctttta ccgctggtgt gccacggtat cgaccgtatc ctctcgcgcc gctcgcccct
     3301 ggtgtttaca ttgacgctgg cagcggggat atgggccaat ttctatattg cctatatggt
     3361 ttgcctgttt tccgttttct actttggcta tgagatgctt cttgagcgca aagaaaaggg
     3421 aaccagagcg gccttttgga aaagccggtg cctgggtttt gcttcgggtg cgctcacggc
     3481 gggcctgctg aacgggatcc tgcttttgcc cgcgctcgaa aacattcaag cgacgaaagg
     3541 ggagctgctg gaatattctt tttcctggca gcagactttc tctttggcgc ggctgccggc
     3601 tcaattgctg ccgggcgggt tcgtgtggca ggacgtggtg gatggcttac ccaatctcta
     3661 ttgcggcctt ttggccgtta tctgcgcgat aggctatttc tgcagcaaaa aaatatcggg
     3721 cagggaaaaa ctgctttcgg gcgcttttct gggaggcctt gttttcacca tgtggatcag
     3781 agggctcgac aatctctggc atggtctgaa acggccggta tggttcccct atcgggagtc
     3841 ttttttgctc tcgtttttaa ttctctttct tgcggccgga gctctgcgcc gggcgtcctt
     3901 ttccagaaaa aaagcggtcc tctttctggt gttgaacgca gcgctccttt ttttggcgct
     3961 gcttttccgc tctcccgcaa tgggcggccg gcgcgctccg ctggcggcgc tcctctggtg
     4021 catggtcata ggattggttg ccgcggcgct gtgggggccg caccggctgc aaaagatcgc
     4081 atttttgggc tgtgttttgg tcacggccgg agaacttacc cttaacgctt attggagtgc
     4141 ggatcagttt gaaaagtact cgcgcagcga ttatcgtact tttgtggagc ggggaacagc
     4201 cgtggtcgac acactgggag caggggcggg aagcaatttc cgcatggaaa aaaatttttt
     4261 ccgcagcttg aacgacccta tgctgctggg atatcacggc accgcccatt ttggctctac
     4321 gcaggatgta gccaacaccc agatgttgaa ctctttggga taccgaagct atgggggcag
     4381 caactcgtac ggctgcggct ccactgcgtt tgcggacgct tttctgggga tccggtatct
     4441 tctctcggat ggggcttttc cagtgggcag tcattatgaa gatatgggcg aaaaagaggg
     4501 atgggagata gaacaaaatc cctatgcctt tcccattggt attttgctgg agaagttgga
     4561 tccgatagag gcggaagagg ggagcggggt ttccactttt gagtttcaaa acaggcttta
     4621 tgccctgttg tccggtgaaa aagatcccct gtttacgccg gtggaaactc tgtccgcttt
     4681 ttgcggcggc gaaccggctt ctgtgacggg gacctttgag gaggagatgg aatatagatt
     4741 taccgcaccg gacgacggac tctattacgc ggtgatgacc agcggttccg ggttgccgct
     4801 ttccctctct gtggagggta agcttgtgga gagcggatat tttacggcgg agcagagcgg
     4861 tgtgatcgac ctgggacggc gggaggcggg ggaaagctgc acttttgctg tggctccacg
     4921 gcaggaaagc gcctcggtgg aggagatcgc aatttaccgc atggacgaaa actcgctgcg
     4981 cgcgctctcg gagcggctga atcgggaaaa cgagggtttg actttgaaag aggggaccgc
     5041 ccatggcatt gtggaggcaa aacaggacgg ttggtttttg atgcagattc cctatgaaga
     5101 agcgctggtg atttcggttg acggcaagga gacgcaagct gtcgaggccg gtggaagttg
     5161 tgtggcggta gccttgaacg cggggaagca cgaactggac gtggcctatc gggcgcccgg
     5221 cgtcacggcg ggagtggtac tctcttttgc cggtgcggca gggcttttag ggctgatcgc
     5281 gtggacaata aaaattgata aaaaagaaca gagagagggg taagtatgcc cgtatgtatc
     5341 attgccgagg ccggggtcaa ccacaacggg gacctggcgc tggcgaaaaa gatggcggtc
     5401 gtggcgaaga acgccggggc cgacatcgtc aaataccaga ccgccgtgcc ggagctggtc
     5461 atcagcaaat tcgccgagaa agccgagtac caaaaggtgc agaccggcga ccatgagacc
     5521 cagctggaga tggtgcgccg catccatttc ggctttgagg cgcaccgcga gctgaaagcg
     5581 tactgcgatt ccatcggcat ccagtatctc tcggccgcct ttgacctgcc cagcatcgac
     5641 tttttggcct cgctggaccc gcccctgttc aagatcccct cgggcgagat caccaacctg
     5701 ccgtatctcg agcgggtggc cgcctgtcac aagccggtgc tgctctccac cggtatgagc
     5761 gagatggagg agatcgccgc cgccctgaac gccctcaagc tggggggcgc gggggaggtg
     5821 acggtgctgc actgcaacac cgagtacccc accccggccg aggacgccaa cctgcgggcc
     5881 atgctggaca tccaaaagcg cttcggctgc cgggtgggct attccgacca cacgctgggc
     5941 atcgaggcgg acatcgccgc cgtggccctg ggcgcccagg tggtggaaaa gcacttcacg
     6001 ctggacaaaa acatggaggg gccggaccac aaggccagcc
//
LOCUS       DBMDYA010000078         6011 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3340_length_6011_cov_1.615567, whole genome shotgun sequence.
ACCESSION   DBMDYA010000078 DBMDYA010000000
VERSION     DBMDYA010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3340_length_6011_cov_1.615567"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            267..2666
                     /locus_tag="ACZPFK_02995"
     CDS             267..2666
                     /locus_tag="ACZPFK_02995"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1405311.1"
                     /translation="MSKKELDQLLRDCLHSEFSVDDLSSATDDQMYRALALVVRRILS
                     EKRKKFMAQTYGENGKQVYYLCMEFLMGRSLKTNLFNLGLNGVADEVLAQYGVKLTNL
                     YELEPDAGLGNGGLGRLGACYLDGMATTSLPGMGYSILYEYGIFRQKIVDGWQQELVD
                     NWLPGGQVWLKSHPEQAVEVRFGGEVHESWDGSYHHIEHKNYDSVLAIPYDMYVSGYD
                     SNGVSQLRLWSAKAPGIDMAAFNQGDYTSALRQNSTAELISKVLYPNDNHVEGKILRL
                     RQQYFLSCASVADIVQRHLAQYGFIENLPDKVAIQINDTHPTLAIPELMRVLLDECGY
                     SWEAAFSMVKRTFAYTNHTVMSEALEKWNVDIFRSTLPRIYEIVAEIDRRAREELMRR
                     FDGDKGKVDYMAMIGDGQVRMANICAYVCHSVNGVSKLHSEIIKESVFHDYYLYKPAA
                     FKNVTNGIAYRRWLLQSNPGLTDLIESAIGTGFKYDAAELSKLTKYKDDASFLDQLDA
                     VKHRNKEIFADYVKRTTGEALNVDSLFDVQVKRLHEYKRQHLNALNIAAQYLWLKENP
                     GADVVPKTYIFGAKAAPGYYMAKQIIRLICALGKVIDSDPAVRDKLKVLYLQDYSVTM
                     SERLMPASEISEQISLAGTEASGTGNMKFMLNGAPTLGTLDGANIEIAAAAGKENFFQ
                     FGMLTEQVNELRRFGYLPNSFIADDEVAVAVLNFIEKGLDGKNFNEVVTNLETQDPYM
                     VMADFRDYRRAQALVSETYRDKRKFSRMSLLNIAGSGVFSADRAVGEYAHNIWHVKPI
                     R"
     gene            2824..3252
                     /locus_tag="ACZPFK_03000"
     CDS             2824..3252
                     /locus_tag="ACZPFK_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405312.1"
                     /translation="MEETIFNSLDEAFKTPFGAVRSGERVTFHLSVPAELGCATPYLC
                     IARADDEQPALLPLRADGQKEGRDLFSIVYTAGAVGAYFYYFDLYVNFTKVFCGDRGE
                     GVLSAEPQGPRYQLTVYEPEFRTPWSARGGVMYQIFPDRF"
     gene            3272..4639
                     /locus_tag="ACZPFK_03005"
     CDS             3272..4639
                     /locus_tag="ACZPFK_03005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1405313.1"
                     /translation="MPFADRYYRPDKDGEPYFWPNEVDGGLLNLDYFGGDFAGIESKL
                     PYLRGMGVTLLYLNPIFEAHSNHRYNTADYMKPDPLLGTEEDFSRLCRAARRLGIRII
                     LDGVFSHTGSDSVYFDKEGRYGSGGAYHNPNSPYRCWYVFGSQYPDGYRSWWGFNTLP
                     EVNEEEPSFRAFICGEGGVIDHWMKLGASGFRLDVADELPDDFIELIRTAVKRHGEDK
                     LLIGEVWEDATNKVSYGVHRTYLLGKGLDTVMNYPFKDAIIQFLRCGDAARLERGLMD
                     IVEHYPKPALDVAMNMLSTHDTMRAITAIAGESCEGRDRYWQSGRALDEPHFFFGKRL
                     MRLGFCLIFTLPGLPCVYYGDEIAMQGYKDPFNRAYFDWNCTDETLRDCVRELAAFRA
                     GCDAFKEGQLRVRKAEGGVFIFERISESGRALVVINRTQAPVVSEIDGKRIPTAEYDY
                     SLFSY"
     gene            5270..5731
                     /locus_tag="ACZPFK_03010"
     CDS             5270..5731
                     /locus_tag="ACZPFK_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1405314.1"
                     /translation="MAAFYPWFWIVLIVLFAVAEGLTVQLVSIWFCIGGVAGLLTSFL
                     TQNFGVQFVVFVVVSAVSLIISWNLFRDRMRPKSVATNADMVIGQEGVVLRAIAPDAG
                     GRVKIDGQDWAAKCDVPLAAGERCKVVSLSGVTLTVAPCVVEKKEETVCGF"
     gene            5719..>6011
                     /locus_tag="ACZPFK_03015"
     CDS             5719..>6011
                     /locus_tag="ACZPFK_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1405315.1"
                     /translation="MWFLIVLAVLLIIFVIILISNIRIVPQACAFVVERFGVYHTTWE
                     AGFHFKTPFIERIAKKVSLKEQVVDFKPQPVITRDNVTMQIDTVVFFQVTDPKL"
BASE COUNT         1155 a         1910 c         1838 g         1108 t
ORIGIN      
        1 cgttgcaaat ggatggtcat acccccatat tataatataa agtatccaat taagaaagtg
       61 cgcggtgcag atttttgtat tgaatcctgt ttttccctgc cggacacccc ccgtttgatt
      121 gaaatgcccc cttaaaaccg atataatagg agaatcatca caagattatg gctgactgcg
      181 ctcataacac ccgcgcccgg cccttggccg tccccggccc ggccgcgcgg gcaatctttc
      241 gagaggtgaa cgatttgaat aaaaaaatgt cgaagaaaga actcgaccag ctgctgcggg
      301 actgcctgca cagcgaattt tcggtggacg acctttccag cgccaccgac gaccagatgt
      361 accgcgccct ggcgctggtg gtgcgccgca tcctcagcga gaagcgcaaa aagtttatgg
      421 cccagaccta cggcgagaac ggcaagcagg tgtactacct gtgcatggag tttttgatgg
      481 gccgctcgct gaagaccaac ctgttcaacc tgggcctcaa cggcgtggcc gacgaggtgc
      541 tggcccagta cggggtcaag ctgaccaacc tctatgagct ggagccggac gccggcctcg
      601 gcaacggcgg cctgggccgc ctgggggcct gttacctgga cggcatggcc accaccagcc
      661 tgcccggtat gggctactcc atcctttatg aatacggcat cttccgccag aagatcgtgg
      721 acggctggca gcaggagctg gtggacaact ggctgcccgg cggccaggtc tggctgaaga
      781 gccaccccga gcaggccgtg gaggtgcgct tcggcggcga ggtgcacgag agctgggacg
      841 gcagctatca ccacatcgag cacaaaaact acgattcggt gctggccatc ccctacgaca
      901 tgtacgtctc gggctacgat tcgaacggcg tctcgcagct gcggctgtgg agcgccaagg
      961 cccccggcat cgacatggcc gcgttcaacc agggcgatta caccagcgcg ctgcgccaga
     1021 actccaccgc cgagctgatc agcaaggtgc tctaccccaa cgacaaccac gtggagggca
     1081 agatcctgcg gctgcgccag cagtatttcc tctcctgcgc ctcggtggcc gacatcgtgc
     1141 agcgccatct ggcccagtac ggctttatcg agaacctgcc cgacaaggtg gccatccaga
     1201 tcaacgacac ccaccccacc ctggccatcc ccgagctgat gcgggtgctg ctggacgagt
     1261 gcggctacag ctgggaggcc gcgttcagca tggtcaagcg cacctttgcc tacaccaacc
     1321 acaccgtcat gagcgaggcg ctggaaaaat ggaatgtgga tatcttccgc tccacgctgc
     1381 cgcgcatcta tgagatcgtg gccgagatcg accgccgcgc ccgcgaggag ctgatgcgcc
     1441 gctttgacgg cgacaagggc aaggtggact atatggccat gatcggcgac ggccaggtgc
     1501 ggatggccaa catctgcgcc tatgtctgcc actcggtgaa cggcgtgtcc aagctgcaca
     1561 gcgagatcat caaggagagc gtctttcacg actattacct ctacaaaccg gccgcgttca
     1621 aaaacgtcac caacggcatc gcctaccggc gctggctgct gcagtcgaac cccggcctga
     1681 ccgacctgat cgagagcgcc atcggcaccg gcttcaaata cgacgcggcc gagctgtcga
     1741 agctgacgaa gtacaaggac gacgcctcct ttttagatca gttggacgcg gtcaagcacc
     1801 gcaacaagga gatcttcgcc gactatgtca agcgcaccac cggagaggcg ctcaatgtgg
     1861 actcgctgtt cgacgtacag gtcaagcggc tgcacgagta caagcgccag cacctgaacg
     1921 cgctgaacat cgccgcccag tatctgtggc tgaaagagaa cccgggcgcc gacgtggtgc
     1981 ccaagaccta tatctttggg gccaaggccg cgccgggcta ctatatggca aagcagatca
     2041 tccgcctcat ctgcgcgctg ggcaaggtca tcgacagcga cccggcggtg cgggataagc
     2101 tgaaagtgct ctatctgcag gattattcgg tcaccatgag cgagcggctg atgcccgcca
     2161 gcgagatctc ggagcagatc agcctggccg gcaccgaggc cagcggcacc ggcaacatga
     2221 aattcatgct caacggcgcg cccaccctgg gcacgctgga cggcgcgaac atcgagatcg
     2281 ccgccgccgc gggcaaggag aatttcttcc agttcggcat gctcaccgag caggtcaacg
     2341 agctgcgccg cttcggctat ctgcccaact ctttcatcgc ggacgacgag gtggcggtgg
     2401 ccgtgctcaa ctttatcgaa aagggcctgg acggcaagaa tttcaacgag gtcgtcacca
     2461 acctggagac ccaggacccc tatatggtca tggcggattt ccgcgactac cgccgcgcgc
     2521 aggcgctggt gtcggagacc tatcgggaca aaaggaagtt cagccgcatg agcctgctca
     2581 acattgcggg cagcggcgtc ttctccgccg accgcgcggt gggcgaatac gcccataaca
     2641 tctggcatgt gaagccgatc cgctgagcgg cggggcggcc ccgggacgcg agagcggccg
     2701 gccaagacgg gacgcaaaaa tatcccctgt ccagggcgac gttttcctgt gtcaacctcc
     2761 ttggccggcc gctctttttg acagcgcccg aggcaaaaaa aattttctga aagaggagcc
     2821 tgcatggaag agaccatctt caacagcctt gacgaggctt tcaagacccc ctttggggcg
     2881 gtgcgcagcg gcgagagggt gacgtttcac ctctcggtgc ccgcagagct gggctgcgcc
     2941 accccctatc tctgcatcgc gcgggcggat gacgaacagc ccgccctgct gcccctgcgg
     3001 gcggacgggc aaaaagaggg gcgggacctc ttttccatcg tctacaccgc cggggccgtg
     3061 ggcgcgtatt tttactactt cgacctctat gtcaacttca ccaaggtctt ctgcggcgac
     3121 cggggcgagg gggttctgtc cgccgagccc cagggcccgc gctaccagct gaccgtgtat
     3181 gagccggagt tccgcacccc gtggagcgcc cgaggcggcg tgatgtacca gatcttcccc
     3241 gaccggttct gagggggtca aaaagaccga gatgcccttt gcggaccgct attaccggcc
     3301 cgacaaggac ggcgagccct atttctggcc caacgaggtg gacggcggcc tgctcaacct
     3361 ggactatttc ggcggcgatt ttgcgggcat cgagagcaag ctgccttatc tgcggggcat
     3421 gggggtcacc ctgctctatc tcaaccccat ttttgaggcc cactcgaacc accgctacaa
     3481 caccgccgat tacatgaagc ccgacccgct gctgggcacc gaggaggatt tctcccgcct
     3541 gtgccgcgcc gcccggcggc tgggcatccg catcatcctg gacggggtgt tcagccacac
     3601 cggctcggac agcgtctatt tcgacaagga ggggcgctac ggctcgggcg gggcctacca
     3661 caaccccaac agcccctatc gctgctggta cgtcttcggc tcccagtacc cggacggcta
     3721 ccgcagctgg tggggcttca acaccctgcc cgaggtcaac gaggaggagc ccagcttccg
     3781 cgcctttatc tgcggcgagg gcggggtcat cgaccactgg atgaagctgg gggcctccgg
     3841 cttccggctg gacgtggccg acgagctgcc ggacgacttc atcgagctca tccgcaccgc
     3901 cgtcaagcgg cacggagagg ataagctgct catcggcgag gtctgggagg acgccaccaa
     3961 caaggtcagc tacggcgtgc accgcaccta cctgctgggc aaggggctgg acacggtgat
     4021 gaactacccg ttcaaggacg cgatcatcca gtttctgcgc tgcggggacg ccgcccggct
     4081 ggagcgcggc ctgatggata tcgtggagca ttaccccaag cccgcgctgg acgtggcgat
     4141 gaacatgctg tccacccacg acaccatgcg ggccatcacc gccatcgcgg gcgaatcctg
     4201 cgaggggcgc gaccgctatt ggcagagcgg ccgggcgctg gacgaaccgc acttcttttt
     4261 cggcaagcgg ctgatgcggc tgggcttctg cctgatcttc acgctgccgg ggctgccctg
     4321 cgtctactac ggcgatgaga tcgccatgca gggctacaag gaccctttca accgcgccta
     4381 ttttgattgg aactgcaccg acgagacgct gcgcgactgc gtgcgggagc tggccgcttt
     4441 ccgcgccgga tgcgacgctt ttaaagaggg acagctgcgg gtgcgcaagg ccgagggagg
     4501 cgtctttatc tttgagcgca tctcggagag cggccgcgcc ctggtggtca tcaaccgcac
     4561 ccaggccccc gttgtcagcg agatcgacgg caagcgcatc cccacggcgg agtacgacta
     4621 cagcctgttc tcttactgag cgcgccttga acgctgtggc gggcaggacc gcagaggctg
     4681 cagaggctgc aggagccgcg gtcaaaggcc gtctgcgaaa gctgtgtttc tcctctcttt
     4741 ctccaccgcc gccctcgctc cgcaatccct gctctgcgcg gtaaggcgga ggcaggcggg
     4801 cgccgcccgg ccgaagcccg cgctgggacg ccccggctcc tttctctttt tggcgccggc
     4861 ggcgcgtctt tttcaaaaat caggggaccc taacaacagg gtttttttgg gggggaagcc
     4921 tcccgccctc tcccagcttt tcctgcgttt tccaaattga caacttgata tcaaaaaaga
     4981 gttgttggtt cagcccggcg gcgcgggggg caccccgggt ccgccccggt gagtagcttt
     5041 cacaaaaacg gcccaaaagg gcaaaatccg tccaaaacgg gggtttgcgg gcattgcgtt
     5101 tcccctccct tttttgttaa actattaaca agaaatccgg taccgggttt cacggggccg
     5161 gcgaacgccg ccgggcccgc tcccttgccg gcggcaacac cgcgggcggg gcaccacaga
     5221 cggcacggcg gggcgcgcgt cccgcctgtc acggaaagga gtttttccca tggcagcttt
     5281 ttatccctgg ttctggatcg tgctgatcgt cctgttcgcc gtcgccgagg ggctcaccgt
     5341 ccagctggtc tcgatctggt tctgtatcgg cggcgttgcg gggctgctca cctcgtttct
     5401 cacccagaac ttcggcgtgc agttcgtggt gttcgtggtc gtctcggccg tgtcgctgat
     5461 catcagctgg aacctgttcc gcgaccggat gcgtcccaaa tccgtcgcca ccaacgccga
     5521 tatggtgatc ggccaggagg gcgtggtgct gcgcgccatc gcccccgacg ccggcggccg
     5581 ggtcaagatc gacggccagg actgggccgc gaaatgcgat gtgccgctgg cggccggcga
     5641 gcggtgtaag gtcgtgagcc tgtccggcgt taccctgacg gtggcgccct gtgttgtgga
     5701 aaaaaaggag gaaacagtat gtggtttttg atcgttcttg cggtgctttt gatcatcttc
     5761 gtcatcattc tcatctcgaa tatccgcatt gtgccccagg cctgcgcgtt cgtggtggag
     5821 cgcttcggcg tctaccacac cacctgggag gcgggcttcc acttcaagac cccgttcatt
     5881 gagcggatcg ccaaaaaggt cagcctgaaa gagcaggtcg tcgatttcaa gccccagccc
     5941 gtcatcaccc gtgacaacgt cacgatgcag atcgacaccg tcgtcttctt ccaggtcacc
     6001 gacccgaagc t
//
LOCUS       DBMDYA010000079         5956 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3373_length_5956_cov_1.372331, whole genome shotgun sequence.
ACCESSION   DBMDYA010000079 DBMDYA010000000
VERSION     DBMDYA010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_5956_cov_1.372331"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="ACZPFK_03020"
     CDS             complement(<1..248)
                     /locus_tag="ACZPFK_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1405316.1"
                     /translation="MEKKIALLPGSGIGAEVLAEADKVLRSAASRFGHRFEFIEAAGG
                     DGAPLDAENLALCLSADSVLAGVGTGVGIRQLIGEMRL"
     gene            complement(1187..2701)
                     /locus_tag="ACZPFK_03025"
     CDS             complement(1187..2701)
                     /locus_tag="ACZPFK_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1405317.1"
                     /translation="MRGIYSSVTDIRRKVFTEVARLAYEGGDYSRIEELPYKIVPGEK
                     ASYRESIFLERAIVGERLRLAIGLPLRPVDEHARLAEGIEDSAVAEKYYEPPLVNIIK
                     FACNSCPDKLVSVTDGCQGCLAHPCKEVCPKGAISMVHGKSVIDQSKCIKCGRCVEAC
                     PYHAIIKQERPCAAACGMNAIGSDELGRASIDYDKCVSCGMCIVNCPFGAISDKGQIF
                     QLIQSIKRGDRVFAAVAPAFVGQLGPKVTPERLKAAMKRLGFADVAEVAVGADLCTID
                     EAEDFVKKVPAEQPFMATSCCPAWSVMAKKLFPDLAPNISMALTPMVLTARLLKKEHK
                     NCKVAFIGPCSAKKLEASRRSVRSDVDFVLTFEEVMGMFEAKGVNFDEITEEHPLREG
                     TAAGRGFAVSGGVAAAVADVIQREHPGHEVKIMAAQGLADCKKMLTIARTGKYDGYLL
                     EGMACPGGCVAGAGTLQPIAKATATVNVYKNQAQEKNALDSKYEITLPLLLEEE"
     gene            4195..5673
                     /locus_tag="ACZPFK_03030"
     CDS             4195..5673
                     /locus_tag="ACZPFK_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR1405318.1"
                     /translation="MDRTQEIERSIIKKYRKSIWNRFVGGVKDYHLIEEGDRIAVCIS
                     GGKDSMLLAKCMQELQRHSYMKFSLEFLVMDPGYNPANRRRIEDNAALLGIPVKIFES
                     DIFEVVEAIAESPCYLCARMRRGHLYKYAQSLGCNKIALGHHFDDVIETVVMSMFYSA
                     EIKTMMPKLRSKNFEGMELIRPLYLVREQDIISWARHNGLSFLQCACRRTEARSDLTG
                     TDGGRRSATKALIRRLRAENPQVDVNIFRSIHDVNLKTVIGYTDLEGGYHHFLDAYEG
                     RTGRPQFAVFDLDGTLLDSMWVWDNVAADYLRAHGVTPPPGLAAQVRGMNVPQAVEFA
                     QREYGVPGTVPQGVRQVCGLVAQAYAEKVQLKPFARDYLDQLRRQGVPMCLATASEPD
                     YVLPALERLGLGGYFRFIATSDSLGATKQSPAYYEKLAERFGAAPQQLTVFDDSLHAI
                     RSAKEAGCRVVAIYDDASLDERSRIKVLCDYYAGGFDALLEG"
BASE COUNT         1077 a         1851 c         2096 g          932 t
ORIGIN      
        1 tgcagccgca tctcgccgat gagctggcgg atgcccaccc cggtgcccac ccccgccagc
       61 acgctgtcgg cggacaggca gagggccagg ttttccgcgt ccagcggcgc gccgtccccc
      121 ccggcggcct cgatgaactc gaaccggtgc ccaaagcggg aggcggccga gcgcagcacc
      181 ttgtccgcct cggccagcac ctcggcgcca atgccgctgc cgggcagcaa tgcgatcttt
      241 ttctccatga tgcgaaacca cctttcctct gtttgaagcc tcggcagagg aaccaaaaaa
      301 cgccccgtct gccgccgaaa ggcgagagac gaagcgtttg ctccgcggta ccactctcat
      361 tgccgcgcag ctgcgcgcgg ccgctcaccc cgtccaacaa cgggcgcccc gataacggag
      421 ggccggccgg cacggcctac tgccccaagg gggttcggcc tgctgctcac aggggatagc
      481 ctcgcggggc cgccgccatc tcgcaccagc cgatggctct ctgaagagcg gttattccgc
      541 ggaccgtgat cctgttcttt gcatttctcg ttacctgtaa gtctatcgcg cacagccgcc
      601 cttgtcaaga aaaatttttt cctccgcagc ggacgcggcg caaacgggca gccaagggag
      661 ggggcaaggg agggggcggc gccgggcccc ggcacggcgg cggagctgac gcggcagaac
      721 agcccggcca cgaccgagag gcaggggcaa cggcgagcgc tagggcgggc cgccgagatc
      781 agaacagccc ggccgcgccc gggaggcaga ggcaacggcg agcggcgtgc ccggcgcctg
      841 tggggcggcg cgggccgaca gcgcggcggc ggtcagggcc gggccggtgt tgtagaggcg
      901 gaaaacagcc ggcagagctt ttgcacccgg gccggcagcc gttcccggct gcgttttcgg
      961 ccgacccgtc gatgcgccgc cattgccccg tttcacggcg tcgtttcctg attcggaggc
     1021 ggccaagaac aaaacggagg agccaaaagg tggataaggg agatgcgggg agaagccgcg
     1081 cgggtcgaaa aaacgacaac cggagagagg accttttcag catgcctccc ccccgctgtg
     1141 gcctgtttct gcgcggccgc agatcgaaag cctgatgccc ctccgctcac tcctcttcca
     1201 gcagcagcgg cagggtgatc tcgtatttgg agtccagggc gttcttttcc tgcgcctggt
     1261 ttttgtacac gttgacggtg gccgtcgcct tggcgatggg ctgcagggtg cccgcgcccg
     1321 ccacgcagcc gcccgggcag gccatgccct ccagcagata gccgtcgtat ttgccggtgc
     1381 gcgcgatggt cagcatcttt ttgcagtccg ccaggccctg ggcggccatg atcttcacct
     1441 cgtgccccgg gtgctcgcgc tgaatcacgt cggccacggc ggcggccacg ccgccgctca
     1501 ccgcaaagcc gcggcccgcg gccgtgccct cacgcagcgg atgctcctcg gtgatctcgt
     1561 caaagttgac ccctttggcc tcgaacatgc ccatcacctc ttcaaaggtg aggacgaaat
     1621 ccacgtcgct gcgcacgctg cggcggctgg cctcgagttt tttggcgctg caggggccga
     1681 tgaaagccac cttgcagttc ttgtgctctt ttttcagcag ccgggcggtg agcaccatgg
     1741 gggtcagggc catcgagatg ttgggcgcca ggtccggaaa cagctttttg gccatcaccg
     1801 accaggccgg gcagcaggag gtggccatga aaggctgctc ggcgggcacc tttttcacaa
     1861 agtcctccgc ctcgtcgatg gtgcacaggt cggcgcccac cgccacctcg gccacgtcgg
     1921 caaagcccag gcgcttcatg gccgccttga gccgctccgg cgtcaccttg gggcccagct
     1981 ggcccacaaa ggccggggcc acggccgcaa agacccggtc gccccgcttg atggactgga
     2041 tgagctgaaa gatctgcccc ttgtccgaga tggcgccaaa ggggcagttg acgatgcaca
     2101 tgccgcagga cacgcacttg tcgtagtcga tggaggcccg gcccagctcg tcgctgccga
     2161 tggcgttcat gccgcaggcc gcggcgcagg ggcgctcctg cttgatgatg gcgtgatagg
     2221 ggcaggcctc cacgcagcgg ccgcacttga tgcatttcga ctggtcgatc acgcttttgc
     2281 cgtgcaccat cgagatcgcg cccttggggc agacctcctt gcagggatgg gccaggcagc
     2341 cctggcagcc gtcggtgacg ctgaccagct tgtccgggca ggagttgcag gcgaatttga
     2401 tgatgttgac caggggcggc tcgtagtatt tttcggccac ggcgctgtcc tcgatgccct
     2461 cggccagccg ggcgtgctcg tccaccggcc gcaggggcag cccgatggcc aggcgcagcc
     2521 gctcgcccac aatggcccgc tcgagaaaaa tgctctcgcg gtaggacgcc ttttcgcccg
     2581 gcacgatctt atagggcagc tcctcgatgc gggagtaatc gcccccctca taggcgaggc
     2641 gcgccacctc ggtgaacacc ttgcggcgga tatcggtcac ggaagaataa atgcctctca
     2701 tcataatcgc agacccgtcc tttctatgat ggcatgggcc cccgccggtg gggcggggca
     2761 aaaatgagtt aaaaatttaa caatcgaaaa ccctttgtaa tcataccatg cgcccttttg
     2821 ccgcgcaagt ggccggatgc cgaaagttgc cccgtttttg cggcaattaa tgggaaaatt
     2881 accgggcttc acgcaaaata cacccaaggg ggaaaagcgt cgggaaaggg agcgggacgg
     2941 gcgcggagag cagagaggac ggcggcgcgc gccgggtagg ggcggcgcag ggggcaaagg
     3001 cccccggggg cgggtcatca cttgtttccg ccttggcggg gcacagcgcg gacgaaagac
     3061 ggagccgggg cggtgtgggg ggcggtggct cccgtatgtc gggttgcgtt tcaaaccggg
     3121 cggagcgctg cgcgggcgcg gagagcagag gccgtcaggg gcaattttcc ctctgcctcc
     3181 atcttggcgc ttggcgcagg cacgcggagc ggaaaagcgc ttttcaaggc caggggcggg
     3241 ttctgcggcg gcccgccaac cgaggggatt tataaaaggc aagcacgaaa gggagacgcg
     3301 gacgggagca ggagcggaaa aaagacggct gaggcggcat gagcgggggc aagggatgca
     3361 cgaggggaaa agaaaggtgg ggccgcagga ctgaaaaaat ggggaagccg gaaaaacatc
     3421 gcaccgaaag caggcgcgag ggagaaagag acggcgggaa cacaagtgac ggcgcaggcg
     3481 cgcggagcgc gaaatgggta cggcggagct gaaagaacgg aggccggagg ggtgcggcgg
     3541 cgcgggcgac atgaggaaaa gaaaaaaacg gcggcacagg gagccgaaga gataaggggg
     3601 gctggagagg cgcgggctga aaagggcggg gcgcggcggg gtctgcgatt tgccctcccc
     3661 agggcggatg tcggccgggg acggcgaaag accgggaaag agcaggggag cgtgagagaa
     3721 caggcgatgg gggtggaggc acccgggagg atgacggctt ccagacgcgc gggcgcagaa
     3781 agagcgccgg gacgagaatg cagcggcgtt ttgctcccgg atcggagagg cgtcgggtgg
     3841 cccgtgtcgt gcgatgggga cggcggcgtt ctgctcctcg gcgcggcgag gcgtcgggtg
     3901 gcccgtgtcc tgcgatgggg acggcggcgt tttgccctca gagcggcgga tgccgggcgg
     3961 cccgtgcgcc tcggcagagg ggcggcgttt tgtcctcgca gcggcggggc gtcggtcggc
     4021 ccgcgtcctg tggcgcgaga cggcggtctt ttgtcccccg ggcggcgggt gccgggcggt
     4081 gccgattgac agagaggcgc aaatcgccta aactggaaaa gtgtccggac aggccccggc
     4141 tccgcgtcgg ggcgggcccg gccgggcaaa atcggacgaa cagcgaggga cgctatggac
     4201 cgcacccagg agatcgagcg cagcatcatc aaaaagtacc gcaaatccat ctggaaccgc
     4261 tttgtgggcg gcgtgaaaga ctatcacctc atcgaggagg gggaccgcat cgccgtgtgc
     4321 atctccggcg gcaaggacag catgctgctg gccaagtgca tgcaggagct gcagcgccac
     4381 agctatatga agttctcgct ggaatttttg gtgatggacc cgggctacaa cccggccaac
     4441 cgcaggcgca tcgaggacaa cgccgcgctg ctgggcatcc cggtgaagat ctttgagagc
     4501 gacatcttcg aggtggtgga ggccatcgcc gagagcccct gctacctgtg cgcccgcatg
     4561 cggcgcgggc acctgtacaa atacgcccag agcctgggct gcaacaagat cgccctgggg
     4621 caccacttcg acgacgtgat cgagacggtg gtgatgagca tgttctactc cgccgagatc
     4681 aagaccatga tgcccaagct gcgcagcaag aactttgagg gcatggagct gatccgcccg
     4741 ctttacctgg tgcgggagca ggacatcatc tcttgggcgc ggcacaacgg gctgtccttt
     4801 ttgcagtgcg cctgccgccg caccgaggcc cgctcggacc tgaccggcac cgacggcggc
     4861 cgccgcagcg ccaccaaggc cctgatccgc cggctgcggg ccgagaaccc gcaggtggac
     4921 gtgaacatct ttcgcagcat ccacgacgtg aacctgaaaa ccgtgatcgg ctataccgat
     4981 ttggagggcg gctaccacca ctttctcgac gcctacgagg gccgcaccgg cagaccgcag
     5041 ttcgccgtgt tcgatctgga cggcaccctg ctggactcga tgtgggtgtg ggacaacgtg
     5101 gccgcggatt acctgcgcgc ccacggcgtc actccgcccc ccggcctcgc cgcccaggtg
     5161 cggggcatga acgtgcccca ggcggtggag tttgcccagc gggagtacgg ggtgccgggc
     5221 accgtgccgc agggggtgcg ccaggtgtgc gggctggtgg cgcaggccta cgccgaaaag
     5281 gtgcagctca agccctttgc ccgcgattat ctggatcagc tgcggcggca gggggtgccc
     5341 atgtgccttg ccaccgcctc tgagccggac tatgtgctgc ccgccctgga gcggctgggc
     5401 cttggcggct atttccgctt tatcgccacc tcggacagcc tgggggccac caagcagtcc
     5461 ccggcttatt atgaaaagct ggcggaaagg ttcggcgccg cgccgcagca gctgaccgtc
     5521 tttgacgact cgctgcacgc catccgcagc gccaaggagg cgggctgccg ggtggtggcc
     5581 atctacgacg acgccagcct ggacgagcgc agccgcatta aggtgctgtg cgactattac
     5641 gccggcggct tcgacgccct gctggagggc tgaggggcac ggatcgggaa aggcccccta
     5701 tttccccggt tctgtggacc tactcaatta tgggctatgg gctgagcgcc acggggcagg
     5761 aaaggccccc tgcccccacc cggggccggg cggctcttga cagggcacag gcgattttag
     5821 aaagcggcgt aaaatcggcc gccccgccgc ccccgctttt agaaaacggg atgcccaagg
     5881 ctcccttgtg caaagggagc tgtcggcgaa gctggctgag ggattgccgg caggcggcct
     5941 ggatatatcc ttgcac
//
LOCUS       DBMDYA010000080         5914 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3397_length_5914_cov_1.448822, whole genome shotgun sequence.
ACCESSION   DBMDYA010000080 DBMDYA010000000
VERSION     DBMDYA010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3397_length_5914_cov_1.448822"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="ACZPFK_03035"
     CDS             <1..867
                     /locus_tag="ACZPFK_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family glycopolymer transferase"
                     /protein_id="HIR1405319.1"
                     /translation="LTRKKAFWLSFACTLGVLIPIYAAALFFGTAHLGSPEAAVAGEG
                     VRIAVRRPDAGDSKNLLLAVGEGENVTFSLVRFDALQGKVCVMPVPSGTRVETEAGKP
                     STVAQQYAYAGPGLAAQQLAALLGVRVDHYFSITRDGLIALGEKMGNISLDLTQPELQ
                     KIGGLPMGSGSGGKTVLSATSAAELIRGSDAGEEALAPLRAEAYSAFLLAGMDRLNTI
                     LPDFLRSNDKSFSTSIFATDIYDYQRILDYLALQQPEMRWGVLKTEPVPGEESAVQVT
                     EAGREQAVELFS"
     gene            complement(1678..2217)
                     /locus_tag="ACZPFK_03040"
     CDS             complement(1678..2217)
                     /locus_tag="ACZPFK_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HIR1405320.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLHIRVGEPLEIYVSGDGEV
                     IFRKYSPLGELSNFAVQYAEVLARATGGAVLVSDRDHVITVSGASKKEFVDQRISQAY
                     EQAMENRRVLSSGDADPIPPFAGAPMSATVLAPILAQGDVIGSVALLGDGQAAQQPGE
                     TEIKLVTVAAAFLGRQMEE"
     gene            2506..3627
                     /locus_tag="ACZPFK_03045"
     CDS             2506..3627
                     /locus_tag="ACZPFK_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1405321.1"
                     /translation="MTDREQQIMELLRQDPMLPQGEIARRLGITRSSVGVHLANLSKK
                     GYILGKGYVLAEQTERYVVGIGAANVDLMGRSREPLVMEDSNPGFISMSVGGVTHNIC
                     ENAARMGAAVKLVTAIGDDVYGEKIRRECEAAGIDTSNFMVAEGESSSTYLSLHDVNG
                     EMSLAMSDMRVLQKLSVEFLKGKHKLLRGASVIVMDAGLPQEVLDYVAATYGGDIPVF
                     ADPVSTTYARKLTGNLHGYHTLKPNRLETEIIAGRSIRSPEELRGACRALIGGGLSRV
                     VVSLGRAGCFYLDAEGRALRAQGRPMDQVVNATGAGDAFMGGLVYASLTGLPPEKTLL
                     FATAASRMAISHQNTINPNISAENVWAVAERDGLTVEEV"
     gene            3705..4622
                     /locus_tag="ACZPFK_03050"
     CDS             3705..4622
                     /locus_tag="ACZPFK_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR1405322.1"
                     /translation="MMNKYLSVSPEIEKALAEGRPVVALESTILSHGMPYPENVEFSH
                     KVEEIVRGEGAVPATTAIIGGKLKVGLTAEELQIMCKADGVGKVSRRDVAVYLATGKT
                     GATTVATTMMLAEMAGIKVFATGGIGGVHRGAETTMDISADLQELANTKVAVVCAGAK
                     SLLDIPKTLEYLETMGVPVLGMGTEDFPAFYCRSSGCKADARIDTPAEMAAILKTKWD
                     LGLKGGVVIGNPIPEQYELDYNEMEAVINRALDAAKAEGVHGKDTTPFLLAHIKDYTE
                     GVSFASNLQLAYNNARVASRIAIELAKLG"
     gene            complement(4986..5849)
                     /locus_tag="ACZPFK_03055"
     CDS             complement(4986..5849)
                     /locus_tag="ACZPFK_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405323.1"
                     /translation="MRQPFPRTTVGGVSLPRMIIGCNWISGFSHRSAAGDTLIRQTHH
                     RPESVSKIFETFLGQGIDAVLGLGDADPNLEKAIALAEERTGKKMILMDEPVLNVDDT
                     AMARAEAKAAIRRCAERGATFCLPLHSCIEQLLNKNTETIDRLPDYLYMIREAGMIPG
                     LSAHMPEVVQYADLNGYDAETYIQIYNCMGFLMQVEIESVNKVIHNAKKPVITIKPCA
                     AGRTTPFVGLNFSYNTIREQDMVCIGCFNENEAAEDIEIARAAIERRPPVIVSRNSPL
                     QTSVIKGVIRD"
BASE COUNT         1119 a         1778 c         1920 g         1097 t
ORIGIN      
        1 ttgacaagaa aaaaagcgtt ttggctcagc ttcgcctgca ccctgggcgt gctgatcccc
       61 atctatgcgg cggcgctgtt cttcggcacc gcgcacctcg gctcgcccga agccgccgtg
      121 gcgggcgagg gggtgcgcat cgccgtgcgc cgccccgacg ccggcgactc caaaaacctg
      181 ttgctggcgg tgggggaggg ggagaacgtc actttttcgc tggtgcggtt tgacgcgctg
      241 cagggcaagg tctgcgtgat gccggtcccc tcgggcaccc gcgtcgagac cgaggcgggc
      301 aagccctcca ccgtggccca gcaatacgcc tatgcggggc ccggcctggc ggctcagcag
      361 ctggcggcgc tgctgggcgt gcgggtggat cactattttt ccatcacccg ggacggcctg
      421 atcgcgctgg gggaaaaaat gggcaatatc tcgctggacc tcacccagcc ggagctgcag
      481 aagatcggcg gcctgcccat gggcagcggc agcggcggca agacggtgct ctccgccacc
      541 agcgcggcgg agctgatccg tggctcggat gccggagagg aagccctggc cccgctgcgg
      601 gcggaggcgt atagcgcttt tctgttggct gggatggacc ggctgaacac gatcctgccg
      661 gatttcctgc gcagcaacga caagtctttt tccaccagta tcttcgccac cgatatctac
      721 gactaccagc gcatcctgga ttatttggcc ctgcagcagc cggagatgcg ctggggcgtg
      781 ctgaagaccg agccggtacc cggcgaggag agcgccgtgc aggtcaccga ggcgggcagg
      841 gaacaggcgg tggagctgtt cagctgagag gggagaaaaa gccccggctg cgaaaaggcc
      901 cgccccaagc aggcccgcct ggggagagcg gcggcggatg taaaggccat cccgcctgtg
      961 gtcggcccgg atgccccaac gcataaaaaa tagccgctgt gcggctattt ttttggccat
     1021 ccgtttcggt tgccgcgcag cggcgagaaa cggggccata aagataaggt gttttttatt
     1081 gcggagcaat aaaaaaatag ccgctgtgcg gctatttttt cggccatccg tttcggttgc
     1141 cgcgcagcga cgagaaacgg ggccatagag ataaagtgtg ttttattgcg gagcaataaa
     1201 aatgagccgc tgtgcggctg ttttttacac gtatgcgcct gccctgcggg ggtgttccga
     1261 gagtttgaaa tcgtgggccg gggctaccta tatggccgtt ttcatagctt gccgcaggca
     1321 ggatgctttt tgtgccaagg tctccgcttg ccccatgagg cgagaggacg gccatgaaag
     1381 taggtccttt aaacagccgc tgtgcggctg ttttttatgc gcatttgacc gctctgcaga
     1441 cgggtgtccc gccggttctc cggcctgggc cgtcaaagta aatcgttttc gttgctttgc
     1501 acggtaaagc ggtaaaaaat ccctttgggc cgcccaggcc catgctctaa agcctttggg
     1561 acgtctaccg gaggattgag ggtatgcgcg cctaaaaccg ccgtcgctct tttgactagg
     1621 ccgcaataaa aagagggcgc gccctgcggc gtgccctctt ttttatgtgg cgaccactca
     1681 ttcctccatc tgccggccga ggaacgcggc cgccaccgtc acgagcttga tctccgtctc
     1741 accgggctgc tgcgccgcct gtccgtcccc cagcagcgcc accgagccga tcacgtcgcc
     1801 ctgcgccagg atgggcgcca gcaccgtggc cgacatcggc gccccggcaa agggagggat
     1861 ggggtccgcg tccccggacg agagcacccg gcgattctcc atcgcctgct cgtaggcctg
     1921 cgagatgcgc tggtccacga actccttttt ggaggcgccg ctcacggtga tgacatggtc
     1981 gcggtcgctc accagcaccg cgccgcccgt ggcgcgggcg agcacctccg cgtactgcac
     2041 cgcaaagttg gagagctccc ccagggggct gtacttgcgg aagatgacct ccccgtcgcc
     2101 gctcacataa atctccagcg gttcacccac ccggatgtgg agcgtgcgcc tgatttcctt
     2161 gggaataacc acccttccca gatcatcgat cctgcgtaca atgcccgtgg ctttcataga
     2221 ctgcttccct ccgttgcttt gttccgtgaa acggaaggct ggccggcttc ctgtttcagc
     2281 tactcctagt atctgccgcg tgagggaata ttatacaaag cgggaaagaa aaaactcccc
     2341 gcagccgctc gcagaaaaga gagaggggat tgtcatatgc gtcaaaaaca agagatcaaa
     2401 tttgtgcgta atgcggatag acaaacgtcc aaaaaacagt acaatataag cataagctta
     2461 attgataagc aaactttaag aaatcaagta aaggagggaa gcgccatgac ggaccgggaa
     2521 cagcagatca tggagctgct gcggcaggac cccatgctgc cgcagggcga gatcgcaagg
     2581 cgtctgggca tcacccgctc ctcggtgggg gtgcacctgg ccaatctgtc caaaaaggga
     2641 tatatcctgg gcaagggcta tgtgctggcc gagcagacgg agcggtatgt ggtgggcatc
     2701 ggcgcggcca atgtggacct gatgggccgc agccgcgagc ccctggtcat ggaggattcg
     2761 aaccccggct tcatcagcat gtcggtgggc ggggtcaccc acaacatctg cgagaacgcg
     2821 gcgcgcatgg gcgcggcggt caagctggtc acggccatcg gggacgatgt ctacggcgaa
     2881 aagatccggc gggaatgcga ggccgcgggc atcgacacct ccaatttcat ggtggcggag
     2941 ggggagagct cgtccaccta cctctcgctg cacgacgtga acggcgagat gtcgctggcc
     3001 atgtccgata tgcgggtgct gcaaaagctg agcgtggagt ttctcaaggg caagcacaag
     3061 ctgctgcgcg gggcgagcgt catcgtcatg gacgcgggcc tgccgcagga ggtgctggac
     3121 tatgtggccg ccacctacgg cggcgatatc ccggtgttcg cggacccggt gagtaccacc
     3181 tacgcccgca agctgacggg caacctgcac ggctaccaca ccctcaagcc caaccggctg
     3241 gagaccgaga tcatcgcggg ccggagcatc cgctcgcccg aggagctgcg cggcgcctgc
     3301 cgcgcgctga tcggcggcgg gctgtcgcgg gtggtggtca gcctgggccg cgcgggctgc
     3361 ttttatctgg acgcggaggg ccgggcactg cgggcgcagg gcaggccgat ggaccaggtg
     3421 gtcaacgcca ccggggccgg ggacgcgttt atgggcggcc tggtctatgc cagcctcacc
     3481 ggcctcccgc cggaaaagac ccttcttttc gccaccgccg cctcccgcat ggcgatctcc
     3541 catcaaaaca ccatcaaccc caacatcagc gccgagaacg tctgggcggt ggccgagcgg
     3601 gacggcctca cggtcgagga ggtttaggcc cgggggtggg cgcggttttg ttttccaggt
     3661 caatcgttac cataaatact gttttttaga aaggaaacgt gaagatgatg aacaagtatc
     3721 tgtctgtttc gcccgaaatt gaaaaggctc tcgccgaggg caggccggtg gtggcgctcg
     3781 agtccaccat tctctcccac gggatgccct atcccgaaaa cgtggagttc tcccacaagg
     3841 tggaggagat cgtgcgcggc gagggggctg tgcccgccac caccgccatc atcggcggca
     3901 agctgaaagt gggcctcacg gctgaggagc tgcagatcat gtgcaaggcc gacggggtgg
     3961 gcaaggtcag ccgccgcgac gtggccgtgt accttgccac cggcaagacc ggggccacca
     4021 cggtcgccac caccatgatg ctggccgaga tggcgggcat caaggtgttc gccaccggcg
     4081 gcatcggcgg cgtgcaccgc ggcgccgaga ccaccatgga tatctccgcc gacctgcagg
     4141 agctggcgaa caccaaggtg gccgtggtct gcgcgggcgc caagagcctg ctggacatcc
     4201 ccaaaacgct ggagtatctg gagaccatgg gcgtgccggt gctgggcatg ggcaccgagg
     4261 atttccccgc gttctactgc cgcagcagcg gctgcaaggc cgacgcccgc atcgacaccc
     4321 ccgccgagat ggccgccatc ctcaagacca aatgggacct gggcctcaag ggcggcgtgg
     4381 tcatcggcaa ccccatcccc gagcagtatg agctggatta caacgagatg gaggcggtca
     4441 tcaaccgcgc cctggacgcc gccaaggcgg agggcgttca cggcaaggac accactccgt
     4501 tcctgctggc ccacatcaag gattacaccg agggggtctc ctttgcctcc aacctgcagt
     4561 tggcctacaa caacgcccgc gtcgcctccc gcatcgccat cgagctggca aagctgggct
     4621 gagacggggc gccgggccct gacgggattc ttttaaatat gaaatacctc ccgcccggac
     4681 cggcagccaa tgccccgccg ccgcggtggg gggactgaaa aaagtgagag cgccctgcat
     4741 ggcagggcgc tctttttgct gctgctttat tgccgcgcaa aaacgatttc tccccgcggc
     4801 cacagtctaa gggcgaggcc gccggactga aaaaagccgt tcggggtgct ttcgccctca
     4861 gccccgaaca gttgagcgtc tccccgcatg gagcgggcga agaagtgcaa aaagcccccc
     4921 aagccgtttt ttgtcggctt gaaaggcttt ttttgttttc cggtgaaaaa gcaagggcgc
     4981 agggctcagt cgcggataac gcccttgatg accgaggtct gaagcgggct gttacggctc
     5041 acgatcaccg gcgggcggcg ctcgatggcg gccctggcga tttcgatgtc ctccgcggcc
     5101 tcgttttcgt tgaaacagcc gatgcagacc atgtcctgct cgcggatcgt gttgtaagag
     5161 aagtttagcc ccacaaacgg ggtggtccgc ccggcggcgc agggcttgat ggtgatgacc
     5221 ggctttttcg cgttgtggat caccttgttg acgctctcga tctccacctg catcaaaaag
     5281 cccatgcagt tgtagatctg gatgtaggtc tccgcgtcgt agccgttgag gtcggcgtac
     5341 tgcaccacct ccggcatgtg ggccgacagg ccggggatca tgcccgcttc acggatcatg
     5401 tagagatagt cgggcaggcg gtcgatggtc tcggtgtttt tgttgagcag ctgctcgatg
     5461 caggagtgca ggggcaggca gaacgtggcg ccgcgctcgg cacagcgccg gatggccgcc
     5521 ttggcctcgg cgcgcgccat ggcggtgtcg tccacgttca gaacgggttc gtccatgagg
     5581 atcatctttt tgccggtgcg ctcctcggcc agggcgatcg ctttttccag attggggtcg
     5641 gcgtcgccca ggcccagcac ggcgtcgatg ccttggccga gaaaggtctc aaagattttg
     5701 ctgaccgact caggccggtg atgggtctgg cggatcaggg tgtcgcctgc ggcgctgcgg
     5761 tggctgaagc cggagatcca attgcagccg atgatcatcc ggggcaggga aacgccgccc
     5821 accgtggtgc gcggaaatgg ttgccgcatc tgtattcaac tccttagaaa tcacttggga
     5881 tcagctgtgc ttaaagaacg taaacgagta tagc
//
LOCUS       DBMDYA010000081         5902 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3401_length_5902_cov_1.597483, whole genome shotgun sequence.
ACCESSION   DBMDYA010000081 DBMDYA010000000
VERSION     DBMDYA010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_5902_cov_1.597483"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            429..587
                     /locus_tag="ACZPFK_03060"
     CDS             429..587
                     /locus_tag="ACZPFK_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405324.1"
                     /translation="MQDLQKFDERMRAYFDTLPADIQNTVLYSDLVLTDLDGLETFAE
                     NMMKLYEQ"
     gene            598..798
                     /locus_tag="ACZPFK_03065"
     CDS             598..798
                     /locus_tag="ACZPFK_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405325.1"
                     /translation="MAMEETVVHRDKAYRSDPALRAYYVALPGEVQIQLCQAAGPITT
                     LGELQSAAEHLKQGWRDQREQG"
     gene            complement(900..1541)
                     /locus_tag="ACZPFK_03070"
     CDS             complement(900..1541)
                     /locus_tag="ACZPFK_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016408.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR1405326.1"
                     /translation="MDMKRNFMQAAGELFGEGKAEEGPTGGSSENKAAHQSAPVPPVA
                     GAGGQKDTSVLTADLTIIGKIEARGNVELNGTLKGDLATQGDVRVFGKVTGNITGQNV
                     TLLSGTVHGDIAAAQRVQVDAKSTVVGDISASDLTAGGRLKGNLKLKKAVTFESTALL
                     LGNVEAQVLAVQEGAKLCGNLQMSHTKEEEAFPPAEEPAGRPAAPGAEKEKGR"
     gene            complement(1581..2387)
                     /locus_tag="ACZPFK_03075"
     CDS             complement(1581..2387)
                     /locus_tag="ACZPFK_03075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1405327.1"
                     /translation="MTQQLEALQAETATVEVNGTLLQETGGIRLQDRTWTDDLAYQEL
                     YPALYCTPPADSVYEEKVIYLTFDDGPSPCTDEILDILAQNGIKATFFVVGRGNDTPQ
                     GRAALQRIVQEGHTLGIHSYSHDYLQIYSSVESFLADFNEMFTYIQQVTGVAPTIFRF
                     PGGSINEYNGGLYREIISEMTRRGFTYFDWNLAARDAATGALTAEEISDNVLERSLSV
                     MRGIVLLHDSSGKQTTVDALQDVITGLQEQGFTFAPLTNEVRPIRFFYRE"
     gene            2452..2718
                     /locus_tag="ACZPFK_03080"
     CDS             2452..2718
                     /locus_tag="ACZPFK_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405328.1"
                     /translation="MAIISVMTARIRCLKKRTLPQYMPLGLSLDKTGRLAAIFVNFTI
                     FKTLFLSFDMKYTKEIAFVTKNDWIKGKSGWKSSRTPCRVEKQK"
     gene            2968..5301
                     /locus_tag="ACZPFK_03085"
     CDS             2968..5301
                     /locus_tag="ACZPFK_03085"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1405329.1"
                     /translation="MSAFSQDILELLRLDYGTSPEQATTVQLYDAVSRAAMAQARGRW
                     RTPAQGKRACYFSAEFLMGRMISANLYNMGLLKECASFLAAHGLDLNVFEEIGDAALG
                     NGGLGRLAACFLDSAATCGVPMDGYGIRYRYGLFAQTFENGFQKEAPDDWQRFTDPWS
                     VRREEDAVLIRFADQTVRAVPYDSPVIGYGAGALVNTLRLWQAEAVRPFDFDLFNRQK
                     YGAAVAERDSAEAISAVLYPNDDTREGKRLRLKQQYFFSAASLADLVRRYKAAHGRDL
                     SGFAEAYAVQLNDTHPTISIPELLRLLMEEGMDFDAAFAVVQKTFAYTNHTIMAEALE
                     KWDVNLFKSVVPQLYPYVELLENALEMELTLRRVPREKKPSYRIICRGQIHMARMAIF
                     ATHSTNGVARIHTEIIKRSALPEWYALWPERFNNKTNGITQRRWLALCNPELSALITQ
                     RIGDGWITDLDQLKRLAPYADDPATQDAFLAVKGEKKRQLAQAIREREGFQLDPTFLY
                     DIQIKRLHEYKRQFMNALSILDLYYGIKEGRITDLLPTAFLFGAKAAPGYYRAKGIIK
                     LINEIARLVDGDEAVRGLLQVAFVQNYNVSYAEKLIPAAEVSEQISTAGTEASGTSNM
                     KFMLNGAVTLGTYDGANIEIVEAAGEENNYIFGARVEDIEKLRQSYDPRKVYDAEPRV
                     RRVLDALVDGTLSDGGTGVFRELYDSILEGASWHRPDQYYLLLDLIPYCETRLRLNRD
                     ARDRRAFAKKCLLNIANAGQFSSDRTIRQYAREIWGV"
     gene            complement(5309..5536)
                     /locus_tag="ACZPFK_03090"
     CDS             complement(5309..5536)
                     /locus_tag="ACZPFK_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405330.1"
                     /translation="MNEKIIHFLKTVIGEKGIKYTVLAERSGIPYQRLMRIFHQGAAI
                     RGSELLALCRQLQIDQAQLMALLDEESRPGV"
     gene            5693..>5902
                     /locus_tag="ACZPFK_03095"
     CDS             5693..>5902
                     /locus_tag="ACZPFK_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1405331.1"
                     /translation="MASKKLGDTLRRLRVLRGYTQQQVADLLGLKNKSTLGSWEVGKS
                     EPDGYTFLRLCRVYEVEDIYAAFEEE"
BASE COUNT         1201 a         1732 c         1777 g         1192 t
ORIGIN      
        1 ccgtgggcgg gcgctcaaga ttttaaggcc gtgggccggg acggcccaca tggccgtttt
       61 tatagctttg cgatgcaaag ctataaaaaa tagccgcagg ctattttttg gccatccgtt
      121 tcggttgccg cagcagcggc gaaaaccgct tttgctgccg ccgccgtttt ttctttcaga
      181 gccgcggacg aggcggttgc agcgagccgc ttttgcctct cccgaccgtc tgatatcaga
      241 ggccgacagg atcagccttt tatcggggag gctttccgct tgatgcccca agggcaaaat
      301 acagcccaca aaaaagggac agccgcagtg cggctgtccc tttttgcgcg gtagcggctt
      361 ggcttgccgg gaccgaggag ggtataagcc ccgcgcaggg gggcaaacta agggcgaggt
      421 gaagagagat gcaggacctg caaaaatttg acgagcgcat gcgcgcttat tttgacacgc
      481 tgcccgcaga catccaaaac acggtgctgt acagcgatct ggtgctgact gatctggacg
      541 ggctggagac ttttgccgag aatatgatga agctgtacga gcagtgagga ggccgccatg
      601 gcgatggaag aaacggtcgt tcaccgcgac aaagcctacc ggtccgaccc ggccctgagg
      661 gcgtattacg tcgcgctgcc cggcgaggtg cagatccagc tctgccaggc ggcgggcccc
      721 atcaccacgc tgggggagct gcagtcagcg gcggagcacc tgaagcaggg ctggcgggac
      781 cagagagaac aggggtagga ccgcagtaga agcatataga aaggcggcgc acaggggagc
      841 ttttcccccg tgcgccgcct ttttgttctg ctttcagccg ccgggccgca agcggcggct
      901 caccggccct tttccttttc cgcccccggg gccgcgggcc gccctgccgg ttcctccgca
      961 ggtggaaaag cttcctcctc ttttgtgtgg gacatctgca gattgccgca cagcttggcg
     1021 ccctcctgca cggcgagcac ctgtgcctcc acattgccca gcagcagggc cgtactctca
     1081 aaggtgaccg ctttcttcag cttcaagttg cctttcagac ggccgccggc agtcaggtcg
     1141 ctggcggaga tatcccccac cacggtggat ttggcatcca cctgcacccg ctgcgccgca
     1201 gcgatgtcgc cgtgtaccgt gcccgagagc agcgtcacgt tttgtccggt gatgttgccc
     1261 gtcaccttgc cgaagacccg cacatccccc tgggtggcca ggtccccctt gagggtgccg
     1321 ttcagctcca cgttgccccg cgcctcgatc ttgccgatga tggtcaggtc ggcggtgagc
     1381 accgaggtgt ctttctgtcc cccggctcca gcgaccggcg ggaccggcgc gctctgatgt
     1441 gcggccttgt tttcacttga gccgcctgtc gggccctcct ctgctttgcc ctccccgaac
     1501 agctccccgg ccgcctgcat aaaatttctt ttcatatcca tgacgatctt cctcctcctg
     1561 tatctgaacc cccaaagggg tcactcccgg taaaaaaagc ggatgggccg cacctcgttg
     1621 gtcagggggg caaaggtgaa cccctgctcc tgcaaaccgg tgatcacatc ctgcagcgcg
     1681 tccactgtgg tctgcttgcc ggaggaatcg tgcagcagca cgatgccccg catcacactg
     1741 agcgagcgct ccagcacgtt gtcagaaatc tcctctgctg tgagggcgcc cgtagcggcg
     1801 tcacgggcgg ccagattcca gtcgaaataa gtaaagccgc gccgggtcat ctcgctgatg
     1861 atctcgcggt agaggccgcc gttgtattcg ttgatgctgc cgccgggaaa gcggaagatg
     1921 gtgggcgcga ccccggtgac ctgctggatg taggtgaaca tctcgttgaa gtcggcgaga
     1981 aagctttcca ccgaggaata gatttgcaga taatcgtggg aatagctgtg gatgcccagg
     2041 gtgtgcccct cctgcacgat gcgctgcagc gccgcccggc cctggggggt atcgttgccg
     2101 cgccccacca cgaaaaaggt ggccttgatg ccgttttggg ccaggatatc caagatttca
     2161 tccgtgcagg gggagggacc gtcgtcaaag gtgagataga tgaccttttc ttcatagacg
     2221 gaatcggcgg gaggggtgca gtagagcgcg gggtagagct cctgataggc gagatcgtcg
     2281 gtccaggtgc ggtcctgtaa acggataccg cccgtttctt gcagcagagt gccgtttacc
     2341 tccaccgtcg ccgtctcggc ctgaagggcc tccaactgtt gggtcaatgt gcggttgtct
     2401 gcatcaagcc tgtgaatccg catcccccaa aggatgcaca gcccggcagg tatggcgatc
     2461 atcagcgtga tgaccgccag aattagatgc ttaaaaaagc ggacgcttcc ccaatacatg
     2521 ccccttggcc tctctttaga caagacaggg cgtcttgccg ccatttttgt aaattttacc
     2581 atatttaaaa cactttttct atcctttgat atgaaatata caaaagaaat tgcttttgtg
     2641 acaaaaaacg actggattaa aggaaagtcg gggtggaaat cctcccgcac gccatgccgc
     2701 gtggaaaaac aaaagtgaaa atgaaaagcg tcaaaatctt ttcagcgcgg catagccaca
     2761 aaggggatcc cggcgacatc gaataattgc cctcaagttc tatatactat ttttatcttc
     2821 tgcgccccgg ccggcacagg cggcgaagat gccgccggag cagggcgcaa tgataagaac
     2881 acagagcgct tttggcggaa tatggaaatc gaaaaaaagc tgtgttacaa taaagccaag
     2941 aatggaacgc ggaaaggaaa gacacacatg agcgcttttt ctcaggatat cctggagctt
     3001 ctcaggctcg attacggcac cagccccgaa caggcgacca ccgtccagct ctatgacgcc
     3061 gtgagccggg cggcgatggc ccaggcgcga gggcgctggc gaacgcccgc gcagggcaag
     3121 cgggcctgct atttttccgc cgagttcctg atggggcgca tgatcagcgc caacctctac
     3181 aacatgggcc tgctcaagga gtgcgcttct tttctcgctg cgcacgggct ggatctgaac
     3241 gtgtttgagg agatcggaga cgcggccctg ggcaacggcg gtctggggcg gcttgcggcc
     3301 tgtttcctcg acagcgccgc cacctgcggc gtgccgatgg acggctacgg catccgctac
     3361 cggtacggcc tgtttgccca gacctttgaa aacggctttc aaaaggaggc cccggacgac
     3421 tggcagcggt tcaccgaccc ctggtcggtg cgcagggaag aggatgccgt actgatccgg
     3481 tttgcagacc agaccgtgcg ggccgtgccc tatgacagcc cggtgatcgg ctatggcgcc
     3541 ggcgcgctgg taaacacgct tcgcctgtgg caggcggaag cggtgcggcc gttcgacttc
     3601 gacctgttca accggcaaaa gtacggcgcc gcggtggcgg agcgcgacag tgccgaggcg
     3661 atcagtgccg tgctttaccc caacgacgac acccgcgagg gcaaacggct gcggctcaag
     3721 cagcagtatt tcttctccgc cgcctctctg gcggatctgg tcaggcgcta taaggccgcg
     3781 cacgggcggg acctctcggg gtttgccgag gcatatgccg tccagctcaa cgacacccac
     3841 cccaccatct ccatccccga gctgctgcgc ctgctgatgg aggaggggat ggattttgac
     3901 gcggcctttg cggtggtgca gaagaccttt gcctacacca accacaccat catggccgag
     3961 gcgctggaaa aatgggacgt caaccttttc aaatcggtgg taccccagct ctacccctat
     4021 gtggagctgc tggagaacgc tctggaaatg gaattgaccc tgcgtcgcgt gccgcgcgag
     4081 aaaaagccga gctaccgcat catctgccgc gggcagatcc acatggcgcg catggcgatt
     4141 ttcgccaccc actccaccaa cggcgtcgcc cgcatccaca ccgagatcat caagcgcagc
     4201 gccctgccgg agtggtatgc cctctggccc gagcggttca acaacaagac caacggcatc
     4261 acccagcggc gctggctggc actgtgcaac cccgagctgt cggccctgat cacccagcgc
     4321 atcggcgacg gctggatcac cgatttagac cagctcaaac ggcttgctcc ctacgcggac
     4381 gaccccgcca cccaggacgc ctttctggcg gtcaaaggag aaaagaagcg ccaactggcc
     4441 caggcgatcc gggagcggga gggcttccag ctggacccga cctttctcta tgacatccag
     4501 atcaagcgcc tgcacgagta caagcgccag ttcatgaacg cgctgtccat cctggacctt
     4561 tactacggca tcaaggaggg gcggatcact gatcttctgc ccaccgcgtt cctctttggg
     4621 gcaaaggccg cgccgggcta ttaccgcgcc aaggggatca tcaagctcat caacgagatc
     4681 gcccggctgg tggacggcga cgaggcggtg cgcgggctgc tgcaggtggc gtttgtgcag
     4741 aactacaatg tgagttatgc cgaaaagctg atccccgccg cagaggtgtc tgagcagatc
     4801 tccacggcgg gcaccgaggc cagcggcacc agcaatatga aattcatgct caacggcgcg
     4861 gtgaccctgg gcacctatga cggcgccaat atcgagatcg tagaggcggc cggggaggag
     4921 aacaactata tcttcggcgc gcgggtggag gacatcgaaa agctgcggca gagctatgac
     4981 ccgagaaaag tttatgacgc cgagccgcgg gtgcgccgcg tgctggatgc gctggtggac
     5041 ggcacccttt ccgacggggg gaccggcgtt ttccgcgagt tgtacgacag cattctggag
     5101 ggggccagct ggcaccggcc cgatcagtat tatctgctgc tggacctgat cccttattgc
     5161 gagacgcggc tgcggctgaa ccgggacgcc cgcgaccggc gcgcctttgc aaaaaagtgc
     5221 ctgctgaata tcgccaatgc agggcaattt tccagcgacc gcaccatccg tcagtacgcg
     5281 cgggaaatct ggggcgtttg aggcggtctc aaacgccggg ccggctttct tcgtccagca
     5341 gcgccatcag ctgggcctgg tctatctgca gctgcctgca cagggccagc agctcgctgc
     5401 cgcggatggc cgccccctgg tgaaaaatgc gcatcagcct ttgataaggg atgcccgacc
     5461 tctcggccag aacggtatac ttgatcccct tttccccgat gaccgttttc aggaaatgaa
     5521 ttatcttttc gttcatcggt ttccacttcc cttttttaaa attgaataat tcatttccaa
     5581 atgcattata cggttgaaaa tatcgtttgt caacgctttt tgattgaatt aatcgttttt
     5641 tgattgaatt ttaaggtttc ctgttgtatg attcattcga agaggtgatt ctatggcaag
     5701 caaaaaactt ggagatactc tgcgcaggtt gcgggtgttg agaggatata cacagcagca
     5761 ggtggcggac ctgctcggct tgaaaaataa aagcacgttg ggctcctggg aggtcggcaa
     5821 atccgagccg gatgggtaca cttttttgcg gttgtgccgg gtctatgagg tggaggatat
     5881 ctatgccgct tttgaggagg ag
//
LOCUS       DBMDYA010000082         5860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3428_length_5860_cov_1.426637, whole genome shotgun sequence.
ACCESSION   DBMDYA010000082 DBMDYA010000000
VERSION     DBMDYA010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_5860_cov_1.426637"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="ACZPFK_03100"
     CDS             complement(<1..318)
                     /locus_tag="ACZPFK_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR1405332.1"
                     /translation="MLFERAAGWLLGKVKFSVEGGSYEKLLNRCTDMGLTLAGLRPTP
                     TGVTGWMPARDYKKIHRAALHCHCRTRVVKKRGLPFLLRRYWGRWGLLLGPAVFVALC
                     LYLP"
     gene            complement(358..753)
                     /locus_tag="ACZPFK_03105"
     CDS             complement(358..753)
                     /locus_tag="ACZPFK_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HIR1405333.1"
                     /translation="MNGSFYRIAARRGKNGKCRQAKGCGGLARGRKKRDGPKKILSSI
                     FQMPPPELYTGAMLHINCNGSVQVDNSKGIVLYNENAVELDMGKTRVRLSGDDLALET
                     VEKGIVLVRGRIFNLEFFYSAREDKKGQG"
     gene            782..1894
                     /locus_tag="ACZPFK_03110"
     CDS             782..1894
                     /locus_tag="ACZPFK_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405334.1"
                     /translation="MNPTGQKASWKNPILAAALLGCAALILLRPDAAAAGIKEGLARC
                     YNSLIPALFPFLILTRLILDSGAARVLGLPFAPYCRLLGIRSPEAGAALAMGLLGGFA
                     AAGCSIDALYRRGEITSRQAEVLLCAAAGEGPAFVVSAVGYAMLGSLRQGTALLCSLM
                     LASLISAPLMALMLPGRTAAQGGAKAAASPSRRQGFAALLVSAVGAAVDTMLRICGFV
                     LLFGLLCSLLIPQNAPGWAAALPAALLEVSAGCKAASLLEGLPALLFCALSLSVLGLS
                     AFAQLRALLSPAIRVLPLACSRLLHFPLTCLLLQLFLRILPGEQAAAAAAAAVERLVL
                     PFRLPREAAFFLFLLLAVFCSELLARTPLRRAGKDV"
     gene            2341..2556
                     /locus_tag="ACZPFK_03115"
     CDS             2341..2556
                     /locus_tag="ACZPFK_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405335.1"
                     /translation="MRNGLFAGEISPACEYCEYGRRASDNVMILCIKKGMVSPYYSCR
                     KFLYSPLKRIPKRQPRLPEFSPEEFQL"
     gene            2553..3011
                     /locus_tag="ACZPFK_03120"
     CDS             2553..3011
                     /locus_tag="ACZPFK_03120"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase family protein"
                     /protein_id="HIR1405336.1"
                     /translation="MSACPNPAAQIVWRELYQRARQAQRPRVLSPFAEAGGVGAALLA
                     ADGSIYTGVCIDMACGLGICAERAAAAQMITCGQSRVLKLAVRMPDGQPGVPCGACCE
                     FLLQMDPENGEAEVLLDPENGHSVKLNSLAPNWWGKRRLPPAPGQAGAAE"
     gene            complement(3603..4643)
                     /locus_tag="ACZPFK_03125"
     CDS             complement(3603..4643)
                     /locus_tag="ACZPFK_03125"
                     /inference="COORDINATES: protein motif:HMM:NF051774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HIR1405337.1"
                     /translation="MRRCAAFLLTLCLCLPLTGCWQPVSLAERAVVKMICVSLRGDQY
                     EVKLAAYVAADGDGESQGESRTVFAQGTGDAVRTALLDAQKGIQQQAFYAQNELLLIE
                     EKAARSHLREILTYFSEERSSRPNMAVFAYRAEQEETLLDKKQVEKTVENLEKMKNGD
                     SVRGGLTQMIYRFDLRGERQDFFLLPRLRVGEEGAVETDRLLFLREGEEILSLKEEEK
                     ALAELLLGGAESYQYEDGEDSCTVSDLRAVFSVPAQGAPKLDLRLSGEVRDAPAGSSP
                     DRLEDRIGRQLEETFSSLYQKCYLEQQLDPFQFGWWFEAQDAGRYGELLRKKELFGEE
                     TVSFAAEITVDP"
     gene            complement(4640..5668)
                     /locus_tag="ACZPFK_03130"
     CDS             complement(4640..5668)
                     /locus_tag="ACZPFK_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015783.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HIR1405338.1"
                     /translation="MEKSDLISPRQLFCCIYLSMVVEMLIKPFSKTIELPAQVLIPAA
                     AVNLLLLALALWPLRAARPDAVARASSTLPGRAVLALMVLTLLLSGGGTLNEANGFLS
                     FTGDWQLSFFWFLPLVLALVYYAVRLGIEGVARVSGVIFVLFLISIGVVVLSNLREMR
                     LQNLAVEAQPFADTAKAVGQGFSFSPALLAAVVLSEKTQGRRGPRLARLLTALFVTYF
                     VFTMVSELVLGGFATAQRQPVYTLARIGGLSVFRRLDSLHSSIWLMALMLKLVVEGAA
                     VELLLRRLLPAKWQKYGVALTVALTGAAGLLFHFSFPTGLRRWLTAAGVLAVLLLALV
                     GRKEKKRV"
     gene            complement(5655..>5860)
                     /locus_tag="ACZPFK_03135"
     CDS             complement(5655..>5860)
                     /locus_tag="ACZPFK_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015292.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1405339.1"
                     /translation="VLLLMLTFFTICSINSFGMPYTAPLTPYGAESLRDGVLRMSWQK
                     MQGHVFTVEDLPGVKGGRRRGKK"
BASE COUNT         1051 a         1955 c         1771 g         1083 t
ORIGIN      
        1 gggcaggtaa aggcagaggg cgacgaacac cgccggccca agcagcaggc cccagcgccc
       61 ccaatagcgg cgcagcaaaa aaggcagccc gcgttttttg accacgcgcg tgcggcagtg
      121 gcagtgcagg gccgcccggt ggatcttttt ataatcccgc gcgggcatcc agccggtcac
      181 ccccgtgggg gtggggcgca gccccgccag cgtaagcccc atgtcggtgc agcggttcaa
      241 caatttttca taagagccgc cctccaccga aaatttgact ttccccagca gccagcctgc
      301 ggcgcgctca aacagcatct ccccgacccc ttttctcaga cagtgctttt ctgcggttca
      361 cccttgccct tttttgtcct ccctggcgct gtaaaagaat tccaggttaa agattctgcc
      421 gcgcaccagc acgatccctt tttccaccgt ctccagcgcc agatcgtccc cggacagccg
      481 cacccgcgtc ttgcccatat ccagctccac ggcgttttca ttatataaga cgatcccctt
      541 gctgttgtcc acctgcaccg agccgttgca gttgatgtgc agcatcgcgc ccgtgtagag
      601 ctccggcggc ggcatctgga agatcgagga gagaattttt ttggggccgt ccctcttttt
      661 gcgccctctt gccaagccgc cacacccttt cgcctgcctg catttgccgt ttttgccccg
      721 ccgcgctgcg atgcgataaa acgacccgtt cacacatata tatcctttgg gggtgcttgt
      781 tatgaatccg accggtcaaa aagcctcgtg gaaaaacccg atcctcgccg ccgccctgct
      841 gggctgcgcg gccctgatcc tgctgcggcc ggacgccgcc gcagcgggga taaaagaggg
      901 tctggcgcgc tgctataaca gcctgatccc cgcccttttc ccctttctca tcctcacccg
      961 gcttatcctg gacagcgggg ccgcccgggt gctggggctg ccctttgcgc cctattgccg
     1021 cctgctgggc atccgcagcc cagaggcggg ggccgcgctg gccatggggc tgctgggggg
     1081 ctttgcggcg gcggggtgca gcatcgacgc gctttaccgg cgcggggaga tcacctcccg
     1141 ccaggccgag gtgctgctgt gcgccgccgc cggggagggg cccgcctttg tcgtctctgc
     1201 agtcggctac gccatgctgg gcagcctgcg ccagggcacg gcgctgcttt gcagcctgat
     1261 gctggcctcc ctgatcagcg cgccgctgat ggcgttgatg ctgcccgggc gcactgcggc
     1321 gcaaggcggc gcaaaggcgg cggccagccc atcccggcgc cagggctttg ccgccctgct
     1381 ggtctccgcg gtgggcgccg cggtggatac gatgctgcgc atctgcggct ttgtgctgct
     1441 gttcggcctg ctctgcagcc ttttgatccc gcaaaacgcc ccgggctggg ccgccgcgct
     1501 gccggccgcc ctgctggagg tcagcgcagg ctgcaaggcc gccagcctgc tggagggcct
     1561 gccggccctg cttttttgcg ccctctccct gagcgtgctg gggctttccg cctttgcaca
     1621 gctgcgcgcc ctgctctccc ccgccatccg cgtgctgcct ctggcctgct cccggctgct
     1681 gcactttccg ctcacctgcc tgctgctgca gctctttctg cgtatcctgc ccggcgagca
     1741 ggccgccgcc gctgcggctg ccgcggtgga gcggctggtg ctcccttttc gcctgccgcg
     1801 ggaggcagcc tttttccttt ttctgctgct cgcggtcttt tgcagcgagc tgctggccag
     1861 gacgccttta cgccgggccg gaaaagacgt ataataaaaa atagccaaag cgaaacagag
     1921 gctgtttttg cagccatggg ggccggttgc ctgccgtaag gcagacgagg gccacggcga
     1981 atcgaaataa tttatttttt attgcgcaaa taataaaaag tagccgcagg ttactttttc
     2041 ggccatccgt ttcggctgtc gcgcggcggc gagaaacggc gtctttcttc gggatcactg
     2101 gtttttacag gtatctgccc gctctgcggg cgagctaaga ttttgaggcc atggccgggg
     2161 cgaccccggc cgcgttgctc tgcaaagctt tcaaagcgga acggagtggg gccgcttttt
     2221 ggggaaagat ttccgcaaaa cttccgcgtc cgcgaccgtg aaactgcctt gcttgcgcgg
     2281 tgaagcgccg ggaaattcca ttagttgtcg gtcggtcgcg gggccagaga ggggtgcgat
     2341 atgagaaacg ggctttttgc cggagagatt tccccggcct gtgaatactg cgagtacggc
     2401 cgcagggcgt ctgacaacgt gatgattctc tgcatcaaaa aggggatggt gtccccctac
     2461 tactcctgcc gcaaatttct atactctccg ctcaagcgga tacccaagcg ccagccccgg
     2521 ctgcctgaat tttcccctga ggagtttcaa ctatgagcgc gtgcccaaac cccgccgcgc
     2581 agatcgtatg gcgggagctg tatcagcggg cgcggcaggc tcagcgcccg cgcgtgctct
     2641 ctccctttgc cgaggcggga ggcgttggcg ccgcgctgct cgccgccgac ggaagcattt
     2701 acaccggcgt ctgcatcgac atggcctgcg ggctcggcat ctgcgccgag cgggccgccg
     2761 ccgcgcagat gattacctgc gggcagagcc gggtgctgaa gctggccgtg cggatgccgg
     2821 acgggcagcc gggtgtgccc tgcggcgcct gctgtgaatt tttattgcag atggacccgg
     2881 aaaacgggga ggccgaggtg ctgcttgacc cggaaaacgg gcacagcgtg aagctgaaca
     2941 gtctcgcccc caactggtgg ggcaagcgcc gtctccctcc ggctcccggg caggcgggtg
     3001 cggcggagtg aggtccgtcg cggcagagcg gcgggcaaaa ccggcagagc tccgcgggac
     3061 gaaaccacgc gagagggcca tcccccctaa agactccaag aaatcaaaaa ggacacggtg
     3121 caaagcaggc tttcgggccc tgctccaaac cgtgtccttt ttatttttat cttttttccg
     3181 cgtattggat cagaacctcc cgcggctgct tttcccgcca ttcacccggt ttgccgcatg
     3241 gcgaaaaatc gaatctttcg atggaaaagc gagtgtgatg aaacggggaa cagtctggac
     3301 cagtgggacg gaaatcatat ggaggtagct ttcccgccgc catgcggcgg ctcttccctg
     3361 aaaaaggtgc cggagagcgc accacaggca gggggtgtgg gcggcaaaaa tatattatgc
     3421 ccgccaaatg gagaagtgca aaggcgcgca aaatttcgtc tccccctggc cgggggaagc
     3481 ggtgcggcga ggccgcgctg gcagagggcc agtcccgccg agagaaaagc ctctgtctca
     3541 caccgccgct ctctctttcg ccccgcggcc ccatctctct ttcacacaag cggaaaagcc
     3601 tttcaggggt cgaccgtaat ctcagcggca aaggacaccg tctcttcgcc gaaaagctcc
     3661 tttttccgca gaagctcgcc atagcgtccg gcgtcctggg cctcgaacca ccagccgaac
     3721 tgaaaggggt ccagctgttg ttcgagatag cacttttgat aaagggagga aaaggtttcc
     3781 tccagctgcc gcccaatgcg atcctccagc cggtcgggcg aactgcccgc aggcgcgtcc
     3841 cgcacctcgc cgctcaggcg cagatcgagc ttcggcgccc cctgggcggg gaccgaaaac
     3901 acggcgcgca gatcgctgac ggtgcagctg tcctccccgt cctcatattg atagctctcc
     3961 gcgccgccca gcagaagctc cgccagggcc ttttcctcct ctttcaggga caggatctcc
     4021 tccccctcgc gcaggaagag aagccggtcg gtctccaccg ccccctcttc tcccacccgc
     4081 agccgcggca gcagaaaaaa gtcctgccgt tcccctctca ggtcaaagcg gtagatcatc
     4141 tgggtcaggc ctccgcgcac gctgtcgcca tttttcatct tttcaagatt ttccaccgtc
     4201 ttttcgacct gctttttgtc caaaagcgtc tcctcctgct cggcgcggta ggcaaagacc
     4261 gccatgttgg gccgggagga gcgctcctcg gaaaaatagg tcaaaatctc ccgcagatgg
     4321 ctgcgggcgg ccttttcctc gatcagcagc agctcgttct gcgcgtaaaa ggcctgctgc
     4381 tgaatcccct tttgggcgtc cagcagcgcc gtgcgcacgg cgtcgcctgt gccctgtgca
     4441 aagaccgttc tgctctcccc ctgagactcc ccatccccgt ccgcggccac ataggccgcc
     4501 agcttcacct catactgatc cccgcgcagc gagacgcaga tcatcttgac caccgcccgc
     4561 tcggccaggc tcaccggctg ccagcagccg gtgagcggca ggcagaggca aagcgtcaat
     4621 aaaaacgccg cacatcgtct catacccttt ttttctcctt tctgcccact aaggccagca
     4681 gcagcacggc cagcacgccc gccgcggtca gccagcggcg cagcccggtg gggaaagaga
     4741 agtggaacag cagcccggcc gccccggtga gggcgacggt cagcgccacc ccgtattttt
     4801 gccatttggc gggcagcagg cggcggagca gcagctccac ggcggccccc tccaccacca
     4861 gcttgagcat cagggccatc agccagatcg aggagtgcag gctgtccagc cgacggaaca
     4921 ccgacaggcc gccgatgcgg gccagggtgt acaccggctg gcgctgggcg gtggcaaaac
     4981 cgcccaagac cagctcactc accatcgtaa agacaaaata ggtgacaaag agcgcggtca
     5041 gcagacgggc cagccggggg ccgcgccgcc cctgcgtttt ttcagacagc accaccgccg
     5101 ccagcagtgc gggggaaaag ctgaatccct gccccacggc ttttgcggtg tccgcaaagg
     5161 gctgagcctc caccgccagg ttctgcagcc gcatctcccg caggtttgag agcaccacca
     5221 cgccgatgga gatcaaaaaa aggacaaaga tcaccccgct cacccgcgcg actccctcta
     5281 tgcccagccg caccgcgtaa tagaccaggg ccagcaccag cggcaggaac cagaaaaagg
     5341 aaagctgcca gtcgccggta aagctcaaaa aaccgttggc ctcgttcagg gtgccgccgc
     5401 ccgagagcag cagggtgagg accatcagcg ccagcaccgc gcgcccgggc agggtgctgg
     5461 aggcgcgggc gaccgcatcc ggccgcgccg cccgcagcgg ccacagcgcc agcgcgagca
     5521 gcagcagatt caccgccgcc gcggggatga gcacctgggc cggcagttcg atcgtcttgc
     5581 tgaacggctt gatgagcatc tccaccacca tggacagata aatgcagcaa aacagctggc
     5641 gggggctgat gagatcactt ttttccacgc cgccgccccc ctttcacccc gggcagatcc
     5701 tccacggtga agacatggcc ctgcatcttc tgccagctca tgcgcagcac gccgtcccgc
     5761 agcgattccg ccccataggg ggtgagcggc gcggtatagg gcatgccgaa gctgttgatg
     5821 gaacagatgg tgaaaaaggt gagcatgagc agcagcacga
//
LOCUS       DBMDYA010000083         5859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3431_length_5859_cov_1.461098, whole genome shotgun sequence.
ACCESSION   DBMDYA010000083 DBMDYA010000000
VERSION     DBMDYA010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_5859_cov_1.461098"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..1238)
                     /gene="tyrS"
                     /locus_tag="ACZPFK_03140"
     CDS             complement(15..1238)
                     /gene="tyrS"
                     /locus_tag="ACZPFK_03140"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1405340.1"
                     /translation="MQLYEELVARGLIAQVTDEETIREMVNAGKATFYIGFDCTADSL
                     TAGHFMALTLMKRLQMAGNKPIALIGGGTTMIGDPSGRTDMRKMLTKEDIDYNAACFK
                     RQMEKFIEFGEGKAMMLNNADWLLGLNYVELLREVGACFSVNNMLRADCYKQRMEKGL
                     SFLEFNYMIMQSYDFYYMFQHYGCNMQFGGNDQWSNMLGGTELIRRKLGKDAHCMTIT
                     LLTDSQGNKMGKTAGNAVWLDPNKTSPFDFYQYWRNVDDADVMKCVRMLTFLPLEQID
                     EMDAWEGEQLNRAKEILAFELTKMVHSEEEALKAQETARALFSGAADRENMPTTEVDA
                     SILNDGAAGLLDLMVAAGLCASKGEGRRLVQQGGVALDGERVENPQQTVPAQRLREGV
                     VFKKGKKIYQRVILK"
     misc_binding    complement(1305..1543)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1573..3240)
                     /gene="recN"
                     /locus_tag="ACZPFK_03145"
     CDS             complement(1573..3240)
                     /gene="recN"
                     /locus_tag="ACZPFK_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1405341.1"
                     /translation="MLNELSIENVAVIEKAEVRFGAGLNVLTGETGAGKSILIDSINA
                     ILGNRTSRELVRSGASKAAVWATFTELPAEARAALEAAGYDAEDELLLYREIGADGKT
                     SCRVNGRPATAAILREIGASLLTIHGQHDNQSLLTPSKHLGILDGYAQHEALLQEYGQ
                     AYRALREVEKQITALSMDEGEKQRRLDLLRYQVEEIEAADLVEGEEEQLTEQRNKIRH
                     SQKILDSLGAAYAALQGGEEFQGGVDLLGEAGGQVEGIGALSEEFSSLAEKLNDLYYS
                     AQDLAAEIKDTLDGFDFDPSQLEEIEGRLDLLHGLKRKYGQSVGEVLSFYEKARAELD
                     TIEFSDQKLAELTERRDRLYRGAAALAERLSRARKKAFEGFSAEIAACLQFLNMPGIR
                     LALACQKVPLGPAGQDDLEFYISTNPGEEPKPLAKIASGGELSRIMLAIKSVMAEKDH
                     IPTVIYDEIDTGVSGLAAGRIGQKLRETAESGHQVICITHTAQIAAYAKSHLLIEKAV
                     ENQRTYTSVRALEEEDRVRELARIISGDRVTELALANAREMLQLAGA"
     gene            complement(3252..3707)
                     /locus_tag="ACZPFK_03150"
     CDS             complement(3252..3707)
                     /locus_tag="ACZPFK_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR1405342.1"
                     /translation="MKNQRHQKILELINAYPIERQEDLLTRLRESGFEVTQATVSRDI
                     RQLRLVKAATGDGHYRYTTPAGQHGQGDKPSRFETIFGESAIQIESAGHIVMVKCHSG
                     MASAACEVFDLVTWEDVVGSLAGENTFIILMRSEEAASRLVVELSKYVK"
     gene            complement(3715..4548)
                     /locus_tag="ACZPFK_03155"
     CDS             complement(3715..4548)
                     /locus_tag="ACZPFK_03155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1405343.1"
                     /translation="MKVLLIVNAAKEEAVRCARRAGEILTRAGAEWSVSQADRPAGDD
                     ACAEGEAVRSCDVVVTIGGDGTLLHAAPQAARWQKPILGVNVGRVGFLATVEATELEL
                     LAKLVENEYTLDERLMLAARVDGGMRYRSDALNDVVVSKGAGTNTIRFDIYCDGTLVN
                     SYRGDGVVIATPTGSTAYSLSAGGPILDARIGGILVTPICAHSLNTPPMVFSAQRSLR
                     IVTAEHRAGGAYLSTDGRDYVALGAKDDILIGLSQKHLSLVSFCEADQFRAIDKKLKG
                     R"
     gene            complement(4545..5363)
                     /locus_tag="ACZPFK_03160"
     CDS             complement(4545..5363)
                     /locus_tag="ACZPFK_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1405344.1"
                     /translation="MESVGSRLDLYLTEHGMVQSRERAKALIMEGLVYLNGEKADKAG
                     MAVPEGAKVELRGQDIKYVSRGGLKLEKAFAEFPLDLNGRVCMDIGASTGGFTDCMLQ
                     NGAAKVYCVDVGYGQLAWKLRCDERVVNLERTNIRYLTEEKVPDKIDFFSVDVSFISL
                     KHIFPVAAALTAANATGVCLVKPQFEAGREKVGKKGVVREAETHREVIESAIGYALAA
                     GFCPAGLSFSPIKGPEGNIEFLLYVTREAGPALEEDRIREVVGQAHAALAEGKR"
     gene            complement(5347..>5859)
                     /locus_tag="ACZPFK_03165"
     CDS             complement(5347..>5859)
                     /locus_tag="ACZPFK_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1405345.1"
                     /translation="FWLLQLLGPQGRGPRAIRYPKGPECPELAALGCSGEPFDCVLHG
                     AAGARVALVSYGLLTREALAAGRALAEAGVPVDVYKVTRLNPLPEKLVPALCAYDRVY
                     AVEDAIASGSLGAHLGLALQRQDYAGRYRNRGLPAGRVDHAEVPQLQKMYGLDRDGII
                     KWIRDDGERG"
BASE COUNT          968 a         2019 c         1692 g         1180 t
ORIGIN      
        1 tgtctgagca ccgctcactt caagatcacg cgctggtaaa tctttttgcc ttttttgaaa
       61 acgacgccct cgcgtaggcg ctgggccggg accgtctgct gggggttctc caccctttcg
      121 ccgtccagcg ccacgccgcc ctgctgtact aggcggcgcc cctcgccctt ggaggcgcag
      181 agcccggccg cgaccatcag gtcgagcagg ccggcagcgc cgtcgttcag gattgaggca
      241 tccacctcgg tggtgggcat attttcccgg tccgccgcgc cgctgaacag ggcgcgcgcg
      301 gtctcctgcg ctttcagcgc ttcctcctcg ctgtgaacca tcttggtcag ctcaaaggcc
      361 agaatctcct tggcgcggtt cagctgctcg ccctcccagg cgtccatctc gtcgatctgt
      421 tccagcggta aaaaggtgag catgcgtacg catttcatca cgtccgcgtc gtccacattg
      481 cgccagtact ggtaaaaatc aaagggactg gtcttgttcg ggtccagcca gacggcgttg
      541 cccgccgttt tgcccatctt gttgccctgc gagtcggtga gcagcgtgat ggtcatgcag
      601 tgggcgtcct tgcccagctt gcggcggatc agctcggtgc cgcccagcat gttggaccac
      661 tggtcgttgc cgccgaactg catgttgcag ccgtagtgct ggaacatgta gtaaaagtca
      721 tagctctgca tgatcatgta gttgaactcc aaaaaggaga ggcctttttc catgcgctgc
      781 ttgtagcagt cggcccgcag catattgttg accgagaagc aggcgcccac ctcgcgcagc
      841 agctccacat agttgaggcc cagcagccag tcggcgttgt tcagcatcat ggccttgccc
      901 tcgccgaact cgataaactt ttccatctgg cgcttgaagc aggcggcgtt gtaatcaata
      961 tcctccttgg tcagcatctt tcgcatgtcg gtgcggcccg aggggtcgcc gatcatggtg
     1021 gtgccgccgc cgatcagggc gatgggcttg ttgcccgcca tctgcagccg cttcatcagg
     1081 gtcagggcca taaaatggcc cgcagtgagg ctgtccgccg tgcagtcgaa cccgatataa
     1141 aaggtcgcct tgccggcgtt gaccatctcg cggatcgtct cctcgtcggt cacctgggcg
     1201 atcagcccgc gggcgaccag ttcttcatac aattgcattt ttattctcct ctcgtacttt
     1261 gggcagaaag caaaacaaaa cgccctccgc caaatcaaaa tttggcagag ggcgaacaat
     1321 tcgcggtacc acctctgttt gccccaacgc tcacgcgagg gggcctttgc gtgttctcaa
     1381 gagaacaact gcgcgctaac gggcgccccc ggcacggcct actgctctgt tcagcctgct
     1441 gctccaagag gtaattcacc tgcggaacgc gcggcccctc gcaccaaccg ggacctctct
     1501 gcgccgctat tccgtggcta ctgggtcttt tcatcgcatt ttctattact atagctgttt
     1561 tacctgtgct tgtcaagcac ccgccagctg cagcatctcg cgggcattgg ccagcgccag
     1621 ctcagtcacc cggtcgccgg aaatgatgcg cgccagctcc cgcacccgat cctcttcctc
     1681 cagcgcgcgg acactggtat aggtgcgctg gttttccacc gctttttcga tcagcagatg
     1741 gcttttggcg taagccgcga tctgggcggt atgggtgatg cagatcacct gatgcccgct
     1801 ctcggcagtc tccctcagct tctgcccgat gcgcccggcg gcgagcccgg acacgccggt
     1861 atcgatctcg tcgtagatca cggtggggat atggtctttt tccgccatca cgctcttgat
     1921 ggccagcatg atgcgggaga gctcgccgcc cgaggcgatc tttgccagcg gcttgggctc
     1981 ttcgcccggg ttggtggaga tataaaactc cagatcatcc tgccccgccg gccccaaagg
     2041 caccttttgg caggcaaggg ccaggcggat acccggcata ttcaaaaact gcaggcaagc
     2101 ggcgatctcg gcgctaaaac cctcaaacgc ctttttgcgc gcgcggctga gccgctccgc
     2161 cagcgccgcg gccccgcggt acagccggtc ccgacgctcg gtcagctccg ccagcttctg
     2221 gtctgaaaac tcaatggtgt ccagctccgc ccgcgccttt tcataaaagg acaagacctc
     2281 ccccacgctc tgcccgtatt tccgctttaa gccgtgaagc aggtccagcc gcccctcgat
     2341 ctcctccagc tgagagggat cgaagtcaaa gccgtccagc gtgtccttga tctccgccgc
     2401 caggtcctgg gcggaataat agagatcatt cagcttttcg gccagggaag aaaactcttc
     2461 cgacagcgcc ccgatcccct ccacctggcc gcccgcctcg cccaacaggt ccacgccgcc
     2521 ctgaaactcc tccccgccct gcagcgcggc ataggcggcg cccaggctgt ccaggatctt
     2581 ctgcgaatgg cgaatcttgt tgcgctgctc cgtcagctgt tcctcctcgc cctcaacgag
     2641 gtcggcagcc tcgatctcct ccacctgata gcgcagcagg tccaggcggc gctgtttttc
     2701 tccctcgtcc atcgagaggg ccgtgatctg cttttcgacc tcgcgcaggg cgcggtaggc
     2761 ctggccgtac tcctgcagca gcgcctcatg ctgggcatag ccgtccagga tgcccagatg
     2821 cttcgagggg gtgagcaggc tctggttgtc gtgctggccg tggatggtga gcagcgacgc
     2881 gccgatctcc cgcaggatgg ccgccgtcgc cggccgcccg ttgaccctgc agctggtctt
     2941 gccgtccgcg ccgatctccc ggtagagcaa aagctcgtcc tccgcgtcat agccggccgc
     3001 ttccagcgcg gcccgcgcct cggcgggcag ctcggtaaag gtggcccata ccgcggcctt
     3061 ggacgcgccg ctgcgcacca gctcgcggct ggtgcggttg cccaggatgg cgttgatgga
     3121 atcaatcagg atcgacttgc ccgcgccggt ctccccggtg agcacgttga gcccggcgcc
     3181 gaagcgcacc tccgcctttt cgatgaccgc tacgttttca atgctcagtt cgttcagcat
     3241 ggcgctgccc tctactttac atatttgctg agctccacga caaggcggct cgccgcctcc
     3301 tcgctgcgca tcaggatgat gaacgtgttc tcccccgcca ggctgccgac cacgtcctcc
     3361 catgtgacaa ggtcaaacac ttcacatgcc gcgctggcca tgccgctgtg gcattttacc
     3421 atcacgatgt ggccggcgct ctcaatctgg atggcgctct cgccgaagat cgtctcaaaa
     3481 cggctgggct tgtccccctg cccgtgctgt cccgccgggg tggtgtagcg gtaatgcccg
     3541 tcgccggtgg ccgccttgac cagccgcagc tggcggatat cccgcgagac ggtcgcttgg
     3601 gtcacctcaa agccggattc cctcagccgg gtcagcaggt cctcctgccg ctcgatgggg
     3661 taggcattga tgagctccaa tattttttgg tgccgctgat ttttcacttc cgcatcatct
     3721 cccctttaat tttttgtcga tcgcacggaa ctggtccgcc tcacaaaagc tgaccagcga
     3781 gagatgcttt tgcgaaagcc cgatcaaaat gtcgtccttt gcgcccagcg ccacatagtc
     3841 gcggccgtcg gtggaaagat aggccccgcc cgcgcggtgc tcggcggtga cgatgcgcaa
     3901 gctgcgctga gccgaaaaga ccatgggcgg cgtgttgagg ctgtgcgcgc agatgggggt
     3961 gaccaaaatg ccgccgatgc gcgcatccag gatgggcccg cctgcggaca gcgaataagc
     4021 tgtggaaccg gtgggcgtag cgatgaccac cccgtcgccg cggtagctgt tgaccagcgt
     4081 gccgtcgcag tagatatcaa aacggatggt gttggtgccc gcgcccttgc tgacgacaac
     4141 gtcgttcaag gcgtcgctgc gatagcgcat gccgccgtcc accctcgcgg ccagcatgag
     4201 ccgttcatcc aaagtatatt cgttttcgac cagcttcgca agcaattcca gctcggtcgc
     4261 ctccacggtg gccaaaaagc ccacccgccc cacattgacg ccgagaatgg gcttttgcca
     4321 gcgcgcggcc tgcggggcag cgtgcagcag cgtgccgtcg ccgccgatcg tgaccaccac
     4381 gtcgcagctg cgcacggctt ctccctccgc acaggcgtcg tcccccgccg ggcggtcggc
     4441 ctgggacaca gaccattccg ctcccgctcg cgtcaggatc tcgccggcgc ggcgcgcgca
     4501 gcgcacggcc tcctccttgg cggcgttgac gatgagcagc actttcatct ttttccctcc
     4561 gccagggccg cgtgcgcctg ccccaccacc tcgcggatgc ggtcctcctc cagggcgggc
     4621 ccggcctccc gggtgacata gagcagaaat tcgatgttcc cctccggccc tttgatgggg
     4681 gagaacgaca gccccgcggg gcaaaaaccg gcggccagcg catacccgat cgcgctttcg
     4741 atcacttccc ggtgggtctc ggcctcccgc accacgccct ttttgcccac cttttcccgt
     4801 ccggcctcaa actgcggctt taccaggcac acccccgtgg cgttcgcggc ggtgagggct
     4861 gcggcgaccg gaaagatgtg ctttaaagag ataaaggaga cgtccacact gaaaaagtcg
     4921 atcttgtccg gcaccttttc ttcggtgagg tagcggatgt tggtgcgctc caggttgacc
     4981 acccgctcgt cgcagcgcag cttccaggcc aactgcccgt agcccacatc cacgcaatag
     5041 acctttgccg ccccgttttg cagcatgcag tcggtgaagc ccccggtgga ggcgccgata
     5101 tccatgcaga cccggccgtt caggtccaac ggaaactccg caaacgcctt ttccagcttc
     5161 aggccgccgc ggctgacata cttgatgtcc tgcccccgca gctccacctt tgcgccctcc
     5221 ggcactgcca tgcccgcctt gtcggctttt tcgccgttga gatagaccaa gccctccatg
     5281 atcagggcct tggcccgctc ccggctctgt accatgccgt gctcggtgag gtaaagatcc
     5341 agcctgctac ccacgctctc catcatccct tatccatttg attatgccgt cccggtcgag
     5401 cccgtacatc ttttgcagct gcggcacctc cgcatggtcg acccgaccgg cgggcagccc
     5461 gcggttgcgg tagcgccccg catagtcctg ccgctgcagc gccagcccca gatgcgcccc
     5521 cagcgagccg gaggcgatgg cgtcctccac cgcgtacacc ctgtcgtaag cgcagagggc
     5581 cgggaccagt ttttccggca gagggttgag ccgggtgacc ttgtagacgt ccaccggcac
     5641 gccggcctct gccagcgccc ggcccgccgc cagcgcttcc cgcgtcagca ggccatagct
     5701 gaccaaagcg acgcgggccc cggcagcgcc gtgcagtacg cagtcgaacg gctcaccgct
     5761 gcagcccagc gccgccagct cggggcactc gggccctttg ggatagcgga tcgcccgcgg
     5821 gcctctgccc tgcggcccca gcagctgcag cagccaaaa
//
LOCUS       DBMDYA010000084         5738 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3516_length_5738_cov_1.411212, whole genome shotgun sequence.
ACCESSION   DBMDYA010000084 DBMDYA010000000
VERSION     DBMDYA010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3516_length_5738_cov_1.411212"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="ACZPFK_03170"
     CDS             complement(<1..331)
                     /locus_tag="ACZPFK_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405346.1"
                     /translation="MVEKAAAVAATETDGPKNSRVAAIFYPQALAPLPLQAVRFAPSC
                     GGALRASLGAAFLRRSFCVLPFLALWATRSLTSAAAPRSMVLSLLAFLPRFSTAGGTA
                     FSFFMLPS"
     gene            complement(543..1415)
                     /locus_tag="ACZPFK_03175"
     CDS             complement(543..1415)
                     /locus_tag="ACZPFK_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR1405347.1"
                     /translation="MTEITESLVFSLLPPRTPEAHKGLYGKLLLVAGSSRYRGAAQLA
                     AAGALRAGAGLVTLASTERVIAAAATALPECTYLPLGEAVTGTIAASSLPALLAAAES
                     CTALAVGCGLAQSPDTAALVLGLAKQIRCPLLIDADGLNLLAGQLDLLKKREIPAVLT
                     PHPGEMARLCGCTAAQVQGAREDAACSLARQTNCVVVLKGHGTLVAAPDGRCFQNTTG
                     GPGLAKGGSGDVLTGLIGGLLAQGLAPVDAAACGVWLHGAAGDACASRRGAVSMLPHE
                     LLDDLCALLAARRV"
     gene            complement(2157..2489)
                     /locus_tag="ACZPFK_03180"
     CDS             complement(2157..2489)
                     /locus_tag="ACZPFK_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR1405348.1"
                     /translation="MKIVYQLCRALSLGVLLGFLGGGLRLLRRPAYSEKQIAAAQRMK
                     SIFAGILKYFTFLFLMLGLVWCFYYLALGLSDPALSEYATNMSQLIVSALTVISILFA
                     FVEFLRKK"
     gene            complement(2648..4246)
                     /locus_tag="ACZPFK_03185"
     CDS             complement(2648..4246)
                     /locus_tag="ACZPFK_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405349.1"
                     /translation="MKKFFALLNVSFRGMLLSANPSSKAKRKAASGVGFLALMCVLML
                     YLGGVYSFAFGAILAPLGRLDLLLALMGLMDVAMCFFFGVMSSSGFVFGGKDNDILLS
                     MPVSAFSVLLSKVLALYLENLAFTLFLLLPAGAACIYFGGFGAAFVLLLVAAAVLLPL
                     LPTALCLLLGFLFSLLQTRVRHRALVTNLLYILFLAAVMVLSFGMSFSMSSGDPSIAG
                     SLDAATGWALPVVWMAKALTGLSLGWMLAFALTCVGSFLLVVWVFSLFYKRILTHLTS
                     VQVRHDYKLTALKSGSAFSALYRKELRRIFGTPVYLMNTGVGALLLLGGGIYAFVSGG
                     DISAVLSQLPPQISGMIPAALALVLCFCVTLSSSTSACISLEGNRLWILREAPVSVDT
                     IFFAKILAGLTLMLPAVLIGAPLGAYGCGLSLTDALVLILLGVAATFFTVLEGLVLNL
                     IWPKLDAVNDTVVVKRSMSVSVQVFGSFGLLAAGAGLYYLLSKPLGPTGALACLAALL
                     ALLSLLFAALLRGWGRRSFEALCG"
     gene            complement(4251..4982)
                     /locus_tag="ACZPFK_03190"
     CDS             complement(4251..4982)
                     /locus_tag="ACZPFK_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405350.1"
                     /translation="MLEIRHFSKTYSGGKRAVDDLNLTVEAGSIYGFIGHNGAGKTTT
                     LRSAAGILGFEEGDILIDGHSVKAEPVAAKQVTAFIPDNPDLYDFLTGIGYLTFIADL
                     YGIPKEQRQTLIAKYGDAFELTGALGSPVAGYSHGMKQKLALISALIRQPKLLILDEP
                     FVGLDPLASHLLKGYLHELCQAGGAVFFSTHVLEVAQKLCDHIAIIKDGRLVESGRTE
                     DVVGNSSLEDVFLELAQQDGENGEA"
     gene            5218..5451
                     /locus_tag="ACZPFK_03195"
     CDS             5218..5451
                     /locus_tag="ACZPFK_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405351.1"
                     /translation="MYKRLRDLREDRNLSQRQVAEMLRMSQTGYSKYETGENDVPTQV
                     LLKLADFYHTSVDYILGRTDTAVPYDDTKNRDR"
BASE COUNT         1230 a         1727 c         1892 g          889 t
ORIGIN      
        1 ttgagggcaa catgaaaaaa gaaaaagctg taccgccagc ggtggaaaaa cgggggagaa
       61 aggcgaggag ggaaagaacc atggaacgcg gcgcagccgc actggtgagg ctgcgagtag
      121 cccacaaggc gagaaacggg agcacgcaaa agctgcgccg cagaaaggct gcccccaaag
      181 acgcccgcaa cgctcctccg caggagggcg caaagcgcac agcttggagc gggaggggcg
      241 cgagggcctg gggataaaaa atagccgcaa cgcggctatt ttttgggcca tccgtttcgg
      301 ttgccgcgac agcggctgct ttttcgacca cgcggcccat ccttgccccg cgggaacgcg
      361 gccataaaaa taagtttttt atttcgaagc agtaaaatgg ccgttatggg actatttttc
      421 tccatccgcc ggctgccgtg tcggcagcga aaagggaagt atcaaaataa aaacgcaccc
      481 cctgcgccga tacaaattgg gacgggggat gcgggagcga aagagagaca gggatcggca
      541 cgtcagaccc gccgcgcggc caatagggcg cacaggtcgt ccagcagctc atgaggcagc
      601 atcgagaccg cgccgcgccg ggacgcgcag gcgtctccgg ccgccccgtg cagccagacg
      661 ccgcaggctg cggcgtccac gggggccagt ccctgcgcca gcaggccgcc gatcaggccg
      721 gtcagcacgt cgccgctgcc gcctttggca aggccggggc cgccggtggt gttttgaaag
      781 cagcgcccgt ccggggccgc cacaagggtg ccgtggccct tgagcacgac gacacagttg
      841 gtttgacggg cgaggctgca ggcggcgtcc tcgcgggcgc cctgtacctg ggcggccgtg
      901 cagccgcaca agcgggccat ctcgcccggg tgcggggtca gcacggcggg gatctcccgc
      961 tttttgagca ggtccagctg cccggccagc agattgaggc cgtccgcgtc gatcagcagc
     1021 gggcagcgga tctgttttgc cagccccaaa acaagggcgg cggtgtccgg gctctgggcc
     1081 agcccgcagc ccaccgccag ggcggtgcag ctttcggcgg ctgcgagcag ggcgggcagg
     1141 gaggaggcgg caatggtgcc ggtcaccgct tctcccagcg gcagataggt gcactcgggc
     1201 agagcggtgg ccgcggcggc aatcacccgc tcggtggagg caagggtgac cagcccggcg
     1261 ccggcgcgca gggccccggc agcggcgagc tgtgccgcac cccggtagcg gctgctgccg
     1321 gcgaccagca acagcttgcc gtacagcccc ttgtgggcct ccggcgtccg cggcggcagc
     1381 agagaaaaaa caagcgattc ggtgatctcg gtcatagaag cttcctttcc ggtaaagggg
     1441 ccttgcccag aggattcttg cgcttttccc gcaagggcgg tttctgctct tggtccatcg
     1501 gagcgctttg gtcgcgcggg ggcgggggac acgagttttc aaaagagcag aatttttttg
     1561 gggggagaga acgggaaaag cgcagggagg atgaggaacc tgcggttttc tcgggcccgg
     1621 caaacctgcc cggcgccggc tgttggcgga aaaggtgcgg ctgcaaaaaa gcggaggccg
     1681 caggcccggt tggcttgcag acggcattgg cacagcgagg gtgcttttcc ggtcggaacc
     1741 agacggcatc cattcccgct cacaagtgcg cccggaagct gctccttttc tgcgggctgg
     1801 acggagcgcc agggcgccta ttttttggag agacgccggg ggagagggga aacgcgactc
     1861 cctgcccctg ccgcttcccc ggcgccagag ggcatccact tttgcttgaa agcgcacccc
     1921 gggctcctct ctcttttgca ggcgggcggt gcgacagcaa gggaagaact gcctttctat
     1981 ttttacagca gccgtccctt ttctgcgggc caaaccgggc ggcaggacgc ccatttgggg
     2041 ggaggcggcg ggaaaagaaa aaacgcagct ccctgtcccc gccgattccc cgagagccga
     2101 aagggcggcg ccgggcatcc tggggcagac ggcccacagc ccaggagaga gaacaatcat
     2161 tttttgcgca gaaactccac aaaggcaaag aggatggaga tgacggtcag cgccgagacg
     2221 atgagctgcg acatgttggt ggcgtattcc gacagggctg gatcagagag acccagcgcc
     2281 agatagtaga agcaccacac cagtcccagc atcagaaaga gaaaggtgaa atattttaag
     2341 atgccggcaa aaatgctttt catcctctgg gcggcggcaa tctgtttttc gctataggcg
     2401 gggcggcgca gcagccgcag gccgccgccc aaaaaaccga gcagcacccc cagcgagagc
     2461 gcgcggcaaa gctgatagac gatcttcatc aaaaggcctc cttgtgaggg cggatccgcc
     2521 cgtgcggcgg gcgtcttcat tgtatcatgt gcgggccaaa aagaacaggc cgcccggcgc
     2581 agtgcgtccg ggcggcctga aaaatatatt gtgggtgaaa aaacgctccg ccccacggcg
     2641 ctgcggtcta accgcagagc gcctcaaagg agcgtcggcc ccagccccgc agcagggccg
     2701 cgaataaaag gctgagcaaa gccagcagag ctgccagaca ggccagtgca ccggtggggc
     2761 cgaggggctt tgagagcaga tagtaaaggc ccgccccggc agccagcagt ccaaacgagc
     2821 cgaagacctg cacgctcacg ctcatgctgc gcttgacgac caccgtgtcg ttgaccgcgt
     2881 ccagcttggg ccagatcagg ttgagcacca gcccttcgag caccgtgaaa aaggtggcgg
     2941 ccacgcccag caggatcagc accagggcgt ccgtcagcga gaggccgcag ccataagccc
     3001 ccagcggcgc gccgatgagc accgctggca gcatcagggt gagccccgcc aaaatcttgg
     3061 caaaaaagat ggtgtccacc gagaccggcg cctcccgcaa aatccaaagc cggttgccct
     3121 ccagcgagat gcaggccgag gtggaggagg agagcgtcac gcagaagcag agcaccagcg
     3181 ccaaggcggc ggggatcatg ccgcttatct gcgggggcag ctggctgagc accgccgaga
     3241 tatcgccgcc gctgacaaaa gcatagatgc ctccgcccag cagcagcagc gcgcccacgc
     3301 cggtgttcat cagatagacc ggcgtgccga agatgcgccg cagctctttg cggtaaaggg
     3361 ccgagaatgc gctgccactc ttgagcgcag tcagcttgta gtcatggcgc acctgcaccg
     3421 aggtgagatg ggtcaggatg cgcttgtaaa acaggctgaa tacccagacc accagcagaa
     3481 aagagccgac gcaggtcagc gcaaaggcca gcatccagcc cagcgagagg ccggtgaggg
     3541 cctttgccat ccagaccacc ggcagcgccc agccggtggc ggcgtccaga gagccggcaa
     3601 tagaggggtc gccggagctc atgctgaagc tcatgccgaa gctgagcacc atcaccgcgg
     3661 cgagaaacag gatgtagagc agattggtga ccagcgcgcg gtggcgcacc cgggtctgca
     3721 gcaggctgaa caaaaagccc aacagcaggc acagggcggt gggcagcagc ggcagcagca
     3781 ccgccgccgc caccagcagc agcacaaagg ccgcgccgaa gccgccgaaa tagatgcagg
     3841 ccgccccggc gggcagcagc aaaaacagcg tgaaagcaag attttcaaga tacagggcca
     3901 gtaccttgga cagcagcacc gaaaaagcgg agaccggcat cgagagcagg atgtcgttgt
     3961 ccttgccccc gaacacaaag ccggagctgc tcatcacgcc gaaaaagaag cacatcgcca
     4021 cgtccatcag gcccatcagg gccagcagca ggtccagcct gcccagcggg gccaggatag
     4081 cgccgaaagc gaagctgtac accccgccca ggtacagcat cagcacacac atcagggcga
     4141 ggaagcccac gcccgaggcc gccttgcgct tggcctttga ggagggattg gccgacagca
     4201 gcatcccgcg gaagctgaca ttcagcagtg caaaaaattt cttcatcgct tcaggcctcc
     4261 ccgttttccc cgtcctgctg ggccagctcc aaaaagacgt cctccagcga ggagttgccc
     4321 accacgtcct cggtgcggcc gctctcgacc agacggccgt ccttgatgat ggcgatgtgg
     4381 tcgcagagct tttgggcaac ctccagcacg tgggtggaga aaaagaccgc gccgcccgcc
     4441 tggcacagct cgtgcagata gcccttgagc agatgggagg ccagcgggtc cagccccacg
     4501 aacggctcgt ccaggatcag cagcttgggc tggcggatga gggccgagat cagggcgagc
     4561 ttctgcttca tgccgtggga gtagccggcc accgggctgc ccagcgcgcc ggtcagctca
     4621 aacgcgtcgc cgtatttggc gatcagggtc tggcgctgct ccttggggat gccgtagagg
     4681 tcggcgataa aggtgagata gccgataccg gtgagaaaat cgtaaagatc ggggttgtcg
     4741 gggataaaag cggtcacctg cttggcagcc accggctcgg ccttgacgct gtggccgtcg
     4801 atgaggatat ccccctcctc aaagccgagg atgcccgctg cgctgcgcag cgtggtggtc
     4861 ttgcccgcgc cgttgtggcc gataaaccca tagatcgacc cggcctccac ggtcaggttg
     4921 aggtcgtcca ccgcgcgctt gccgccgctg taagtcttgg aaaaatgacg gatctccagc
     4981 ataatactcc tctttccgga ccgccgctgc ggccctttac accgaactta caattagaaa
     5041 aatgtctaaa tgtgacctca ttatagcgtc cgcccccggg gttgtcaagc gggggaaaaa
     5101 ggcaggggcg acgcagcaga gatggtcatt cctctgtttt gattatttta acctgtttca
     5161 ggttaatagt caatgtctga gggaaaactg ggatagaatt tatccgaggt gtttgccatg
     5221 tacaagaggc ttcgcgacct gcgggaggac cggaacctgt cccagcggca ggtggcggag
     5281 atgctgcgca tgtcccagac gggatattca aagtatgaga cgggagaaaa cgacgtgccc
     5341 acgcaggtgc ttttgaagct ggcggatttt taccacactt cagtggacta tatcctgggc
     5401 cgcaccgaca cggccgtgcc ctatgatgac acgaaaaaca gagacagata gcgggcggtg
     5461 gctgaaacag gcttttatcc gcccgctgtt ttgtttttgt cgttttgcgg caaagcggcc
     5521 tgccggacgg ccttggggct gtaaattggc ggagggcgaa gcaggcggcg cgttcctcac
     5581 cgcagctgta acgcccggac ttctccggtg cggcgctgcc gcgccgcccg tggtcagggg
     5641 aggcagagcg cgcgccttgc ggctttcgcg gaatgggaga ctctgcgctc agcacggttc
     5701 ccatagggaa cgactgtcgc ctgctttcag cggaaaga
//
LOCUS       DBMDYA010000085         5731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3519_length_5731_cov_1.335702, whole genome shotgun sequence.
ACCESSION   DBMDYA010000085 DBMDYA010000000
VERSION     DBMDYA010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_5731_cov_1.335702"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="ACZPFK_03200"
     CDS             <1..679
                     /locus_tag="ACZPFK_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1405352.1"
                     /translation="LQIFLNDSYVDSSGTNNCANIICDPWESSFVAGFVAAMTTKKDV
                     VGWIGHTDSPTQDRFRYGYEAGVKYAGNGKEVVVAYTGDSNDSVKGQEAAKAMIANYG
                     VDTIYQAANTSGLGVILECKEAGIHCIGVDMWQGGEYGQETVFWSALKPINDAINECV
                     RAAQAGEWEGNMFYYDLAHGAKVYDQRDFDLLDKDLQQKVLDLTKEIAEGKIDVYEGY
                     DDYRYQA"
     gene            844..2373
                     /locus_tag="ACZPFK_03205"
     CDS             844..2373
                     /locus_tag="ACZPFK_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405353.1"
                     /translation="MENERQDYVLETRNICKNYALLKANDHISLKVRRNTIHAIVGEN
                     GAGKSTLMGILTDVVKPDMGDIILNGEKMNFKNPMDAAHHGIGMIYQEFMLAPRFTVF
                     ENIIVGFEEKKGIFVDRKKCRERVEAICERYHFSLPLDELIDELPVSVLQQIEIVKVL
                     YRGADLIIMDEPTSTLTPQGIEGLFDAMRVLKESGKTILFITHKLGEVFRVSDEISVL
                     RDGKLVGTYQPQEVDQQKLANLMVGREVILQAHKLPVKAGQPVLEVKNLRVRDADGIE
                     RVKDANFEIRAGEIVGIAGVAGSGQQFLVEALFGLAKPETDSVIRYLGEEVTGKSPRD
                     HRADRVGYVPQDRIGMGCNVAASIWENAIMGYHRATGFHPKWLIPRRQAMEFTDRVVQ
                     DFDVKVQNLENHIGSLSGGNIQKLIVGREFSQNNRLLLIEDPTRGIDVGAIEYIWEKL
                     IRFAAEGAAVLLVSHDLNEVMQLSDRILVMYDGRLRDGGGHGELTEEQIGLLMMGGEV
                     G"
     gene            2370..3473
                     /locus_tag="ACZPFK_03210"
     CDS             2370..3473
                     /locus_tag="ACZPFK_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405354.1"
                     /translation="MKKLRLTLSEDGKISLMKYGLSLIATLAVGAVMILLQGENPATA
                     FVEIFRGAFGSKAAIGNTLHWAVPCMLVGSAALVAFKSGVMNLGLEGQLYLGGFVAGC
                     AGYMVSLPPVLHPLFCLLAGGLAGMLWAMLPAFLKLLFKVDEVITTLLLNYVAIFFTE
                     LLTLLVISGAKALGTNSIMTPLIHDSAKLTPLISGTSATTGIFVAVAVVLCVYLLYKY
                     TLTGYELKQVGENLKFARVGGVNANKMFIIIFLVSGFIAGLCGSAETIGVNNRFISRF
                     SSNLGWDGVMITRVASNSPLGVLVVSLIWAAFKAGALHMERTTTLNRYIINLLQALFV
                     LFISIDYGMLYRKYLSWRARKKMVEEEARKSES"
     gene            3582..>3963
                     /locus_tag="ACZPFK_03215"
     CDS             3582..>3963
                     /locus_tag="ACZPFK_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1405355.1"
                     /translation="MEYIQAMYSTITLSATFRLSAPLLLGLAGYCFSNKAGIMNIALE
                     SFMMISAFFALLGSYLFHSAFIGALFGVVAGTVLSFLYGVLVFHAGANGLITGVGFNL
                     SGWGLTTMLLVALFHTRGATNVGAV"
     assembly_gap    3964..3973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3974..4472
                     /locus_tag="ACZPFK_03220"
     CDS             <3974..4472
                     /locus_tag="ACZPFK_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1405356.1"
                     /translation="YLGKIFNQQTILVYLGPLLMLVAWVVMYKTPFGVRIRAVGLNQR
                     AAETAGVSVMKYSWIAMLLSGACMGLSGTFISLNSLAMFSENMTGGRGFMVLASAMVA
                     GGNPLKAMLCGFIFAYCDALTLTWSGFDVYSQVISMLPYLAVLVVLFLTHLKNIKSFG
                     RFEAS"
     gene            4662..5684
                     /locus_tag="ACZPFK_03225"
     CDS             4662..5684
                     /locus_tag="ACZPFK_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1405357.1"
                     /translation="MTVRQSKILGGLLAAAVGDAMGAATEIRTTGMIKERFGGYVTDI
                     VQPPEDTWAHWAKAGMVTDDFSVSYFSAEDILKNGGVVTEEVAAQAVVRWSEHPLYSQ
                     CAGPTTRAAIAKIKGIPLPPDKHDRLLCHNSRASNGAPMKVGCVGLFDACDVDKAIDD
                     ALTMCKVTHDNTIALSGAAAIAAATARAMDSRVSYLEVIQAGVYGAVEGFKRAESFAR
                     PVAGASVEKRIYRAVEIGLRCQGDFEKAMTVLSEEIGGGLPANEAVPAAFGYIAATAG
                     DVMQTIHMAVNAGDDTDTVACMAGYIVGALGGVERIPARYLQTIEKMNGFELQKLALG
                     IDALLK"
BASE COUNT         1101 a         1689 c         1864 g         1067 t
ORIGIN      
        1 cctgcagatc ttcctcaacg actcctatgt ggactcctcc ggcaccaaca actgcgccaa
       61 catcatttgt gacccgtggg agtcctcgtt tgtggccggc tttgtggctg ccatgaccac
      121 gaaaaaggat gtggtgggct ggatcggcca caccgacagc cccacgcagg accgcttccg
      181 ctatggctat gaggcgggcg tgaaatacgc gggcaacggc aaagaggtcg tggtggccta
      241 caccggtgac tccaacgact cggtcaaggg gcaggaggcc gccaaggcca tgatcgccaa
      301 ctacggcgtg gacaccatct atcaggcggc caacacctcc ggcctgggcg tcatccttga
      361 gtgcaaagag gccggcatcc actgcatcgg cgtggatatg tggcagggcg gcgagtacgg
      421 ccaggagacc gtgttctgga gcgcgctgaa gcccatcaac gacgccatca acgagtgcgt
      481 gcgggctgcg caggccggcg agtgggaggg caacatgttc tattatgacc tcgcccacgg
      541 cgccaaggtc tacgaccagc gcgactttga cctgctcgac aaggacctgc agcagaaagt
      601 ccttgacctg accaaagaga tcgcggaggg aaagatcgac gtctacgagg gctacgacga
      661 ctaccgctat caggcgtgag ggcgcacggg cgaagctgag tgctgaaaaa atggggcgaa
      721 agcgcggttt tcgccccatt tttaaagggg cggacggggt ctcgcgcgca ccggccgcgt
      781 gggatgccgg gcccgcaggc tttgcccctg agtttgcttc gcagggaaag gacggagctg
      841 tgaatggaga atgaaagaca agattatgtg ctcgagaccc gcaacatctg taaaaattat
      901 gcccttttga aagcgaacga ccacatctcg ctgaaagtgc gccgcaatac catccacgcc
      961 atcgtgggcg agaacggcgc gggcaagagc acgctgatgg gcattttgac cgacgtcgtc
     1021 aagccggaca tgggggacat catcctcaac ggggaaaaga tgaattttaa aaaccccatg
     1081 gacgccgccc atcacggcat cggcatgatc tatcaggagt tcatgctggc gccccgcttc
     1141 acggtgttcg agaacatcat cgtgggtttt gaggagaaaa agggcatctt tgtggaccgc
     1201 aaaaaatgcc gcgaaagggt ggaggccatc tgcgagcggt accatttcag cctgccgctg
     1261 gatgagctga tcgacgagct gccggtgtcg gtgctgcagc agatcgagat cgtcaaggta
     1321 ctctaccgcg gcgcggacct gatcatcatg gatgagccca cctccaccct gaccccgcag
     1381 gggatcgagg gcctgttcga cgccatgcgg gtgctgaaag agtccggcaa gaccatcctc
     1441 ttcatcaccc acaagcttgg ggaggtcttt cgggtcagcg acgagatctc ggtgctgcgg
     1501 gacggcaagc tggtgggcac ctatcagccg caggaggtgg accagcagaa gctggcgaac
     1561 ctgatggtgg gccgcgaagt gattttgcag gcgcacaagc tgccggtcaa ggcgggccag
     1621 ccggtgctgg aggtgaaaaa cctgcgggtg cgcgacgcgg acggcatcga gcgggtgaaa
     1681 gacgcgaatt ttgagatccg cgccggcgag atcgtcggta tcgcgggcgt ggcgggctca
     1741 gggcagcagt ttttggtgga ggcgctgttc ggcctggcaa agcccgagac cgacagcgtg
     1801 atccgctatc tcggcgagga ggtgaccggc aaatcccccc gcgaccaccg ggcggaccgg
     1861 gtgggctatg tgcctcagga ccgcatcggc atgggctgca atgtggccgc aagcatctgg
     1921 gaaaacgcca tcatgggcta tcaccgggcc accggctttc acccaaaatg gctcatcccg
     1981 cgcaggcagg cgatggagtt caccgaccgg gtggtacagg atttcgacgt caaggtgcag
     2041 aatctggaga accatatcgg ctcgctctcc ggcggcaata tccaaaagct gatcgtgggc
     2101 cgcgagtttt cgcaaaacaa ccggcttctg ctcatcgagg accccacccg gggcatcgac
     2161 gtgggagcta tcgagtacat ctgggaaaaa ctgatccggt ttgcggccga gggggccgcg
     2221 gtgctgctgg tcagccatga cctgaacgag gtgatgcagc tgtccgaccg gatcctggtc
     2281 atgtacgacg ggcggctgcg ggacggcggc ggacacggcg agctgaccga ggagcagatc
     2341 ggactgctga tgatgggcgg tgaagtggga tgaaaaaatt gaggcttaca ctgagtgagg
     2401 acggcaagat cagcctgatg aaatacggcc tgtccctgat cgcaacactg gcggtgggcg
     2461 ccgtcatgat cctgctgcag ggagaaaacc ccgccaccgc ttttgtggaa attttccgcg
     2521 gggcattcgg ctccaaagcg gccatcggca acaccctgca ctgggcggtg ccctgcatgc
     2581 tggtgggctc ggcggcgctg gtcgccttta aatccggcgt catgaacctg ggcctggagg
     2641 gccagctgta tctgggcggt tttgtggcgg gctgcgcggg ctatatggtg tcgctgcccc
     2701 cggtgctgca cccgctgttc tgcctgctgg ccggcggcct ggcgggcatg ctctgggcca
     2761 tgctgcccgc ttttctcaag ctgcttttca aggtggacga ggtcatcacc accctgctgc
     2821 tcaactatgt ggcgatcttt ttcaccgagc tgctcactct gctggtgatc tccggcgcaa
     2881 aggcgctggg cactaacagc atcatgaccc cgctcatcca cgacagcgcc aagctgaccc
     2941 cgctcatctc gggcacgtcg gccaccaccg gcatctttgt ggcggtggcg gtggtgctct
     3001 gcgtctatct gctctacaag tacacgctga ccggctatga gctcaagcag gtgggcgaaa
     3061 atttgaaatt cgcccgtgtg ggcggcgtca acgccaataa gatgttcatc atcatctttt
     3121 tggtctccgg ctttatcgcg ggcctgtgcg gctccgccga gaccatcggc gtcaacaacc
     3181 gcttcatctc ccgcttcagc tccaacctgg gctgggacgg cgtcatgatc acccgcgtgg
     3241 cctccaacag cccgctgggc gtgctggtgg tctcgctgat ctgggcggct ttcaaggcgg
     3301 gcgcgctgca catggagcgc accaccaccc tcaaccgcta tatcatcaac ctgcttcagg
     3361 cgctgttcgt gctgttcatc tcgattgatt acggtatgct gtaccgcaaa tatctcagct
     3421 ggcgggcgcg caaaaaaatg gtggaagagg aggcgcgcaa aagtgaaagc tgatcttttg
     3481 atctgcggga tgcagaaaaa ctgcggccgc accccggcgc cggacaaacc gcggcggtgc
     3541 cgtcgcaaag gttcggccgc tgcgaaagga ggagtataaa aatggaatac attcaggcca
     3601 tgtactccac catcaccctc agcgcgacgt ttcgcctgag cgccccgctg ctgctggggc
     3661 tggcgggcta ctgcttttcc aacaaggcgg gcatcatgaa catcgccctg gagagcttta
     3721 tgatgatctc cgctttcttt gccctgctgg gcagttatct gtttcacagc gctttcatcg
     3781 gggcgctgtt cggcgtcgtc gccggcacgg tgctctcgtt cctgtacggg gtgctggtct
     3841 ttcacgccgg ggcgaacggt ctgattaccg gcgtgggctt caacctgagc ggctggggcc
     3901 tgaccacgat gctgctggtg gcgctcttcc acacccgggg cgccaccaac gtgggcgcgg
     3961 tcannnnnnn nnnatatctc ggcaagatct tcaaccagca gaccatcctc gtctatctgg
     4021 ggccgctgct gatgctcgta gcctgggtcg tgatgtacaa gactcctttc ggggtgcgca
     4081 tccgcgcggt gggcctcaac cagcgcgctg ccgagacggc gggcgtgagc gtcatgaaat
     4141 attcctggat cgccatgctg ctctccgggg cctgcatggg tctgtccggc acctttatct
     4201 cgctcaattc gctggccatg ttctctgaaa acatgacggg cgggcgcggc tttatggtgt
     4261 tggcctcggc gatggtggcg gggggcaatc cgctcaaggc aatgctctgc ggcttcatct
     4321 tcgcctactg cgacgcgctg accctgacct ggtccggctt cgacgtctac agccaggtca
     4381 tcagtatgct gccttatctg gcggtgctgg tggtgctgtt cctcacccac ctgaaaaaca
     4441 tcaaaagctt tgggcgcttt gaggcgtcgt agcgggcggg tgaaaaagca aaaaggcggt
     4501 tcggcgcctg gagcctgcct ttttcgggct ccggcttcag gtcgaaattt tgccgcaaag
     4561 gcggcggggg caaaggcgcg ccccggttcc gcattgccgg ggcgggcccg cgccctgccg
     4621 ccgcgcggca aagagcatca ttttatttag gaggttaagg catgactgta cggcaatcta
     4681 aaattctcgg cgggctgctc gcggccgccg tgggggacgc gatgggtgcg gcgaccgaga
     4741 tccgcaccac cggcatgatc aaagagcggt tcggcggcta tgtcaccgac attgtgcagc
     4801 cgcccgagga cacctgggcc cactgggcca aggcgggcat ggtgacggat gatttttccg
     4861 tctcgtattt ctccgccgag gacatcctca aaaacggcgg cgtggtgacc gaggaggtgg
     4921 cggcacaggc ggtggtgcgc tggagcgagc acccgctgta ctcccagtgc gcgggcccca
     4981 ccacccgcgc ggccatcgcc aagatcaagg gcatcccatt gccgcccgac aagcacgacc
     5041 ggctgctgtg ccacaacagc cgcgcctcca acggcgcgcc gatgaaagtg ggctgcgtgg
     5101 gcctgttcga cgcctgcgac gtggataagg ccattgacga cgccctcacc atgtgcaagg
     5161 tgacccacga caacaccatc gccctgtcgg gcgctgcggc catcgccgcc gccacggcgc
     5221 gggcgatgga cagccgcgtg agctatctgg aggtgatcca ggcgggcgtc tatggcgccg
     5281 ttgagggctt caagcgggcg gagagctttg ctcgcccggt ggccggggcc agcgtggaaa
     5341 agcgcatcta ccgcgcggtg gagatcggcc tgcgctgcca gggcgatttt gaaaaggcta
     5401 tgacggtgct ctcggaggag atcggcggcg gactgcccgc caacgaggcc gtgccggcgg
     5461 cgttcggcta tatcgccgcc accgcgggcg acgtcatgca gaccatccac atggcggtca
     5521 acgcggggga cgacaccgac accgtggcct gcatggcggg ctatatcgtc ggcgcgctag
     5581 gcggggtgga acgcatcccg gcccggtacc tgcagaccat cgaaaagatg aacggctttg
     5641 agctgcaaaa actcgcgctc ggcatcgacg cgctgctgaa gtagggagga atgacaagat
     5701 gacgacaaag tatgaccgga tcatgggcgc c
//
LOCUS       DBMDYA010000086         5676 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3552_length_5676_cov_1.570942, whole genome shotgun sequence.
ACCESSION   DBMDYA010000086 DBMDYA010000000
VERSION     DBMDYA010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3552_length_5676_cov_1.570942"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    739..748
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <749..1281
                     /locus_tag="ACZPFK_03230"
     CDS             <749..1281
                     /locus_tag="ACZPFK_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405358.1"
                     /translation="LWKQEDDEQTQAHKRAYGNYLKNIRPAAQSPAAETAVVKDAGLA
                     AAISGGQGADLLGSTAKGLGITAGATAATGAAGPSLAAGLNAAEAYFLAYEDAVRQGL
                     PVEEAKKKAWENVVLSMGADQLVGSFGEYFDWGDVSGEGINTLLHKLLDLALSSSAKG
                     DAEDEDKTDKAAQPGF"
     gene            complement(1219..1575)
                     /locus_tag="ACZPFK_03235"
     CDS             complement(1219..1575)
                     /locus_tag="ACZPFK_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405359.1"
                     /translation="MLLVVCTLLFAFGDLIFPVFCRLFWGPERCFRRRDLLAGVNVAL
                     CTLLAALSIYFCEGLPAPSPRTVSIFLLRSLVGSPAVAGFIPFRLLSIQSWKDFSKAR
                     LRCFVGFILILSVAFR"
     gene            1785..2297
                     /locus_tag="ACZPFK_03240"
     CDS             1785..2297
                     /locus_tag="ACZPFK_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405360.1"
                     /translation="MTLGQAKERLKQLLDESKPKADLTGKLDRFFDMGQKEVALYYPI
                     YRTKEYAAGEARALPGDFFRGCQVVADGVWHAYDPAAALPQGAFTLRYEARPADIPEN
                     APDSLELEVAEEAASAVLFYVAAQCNSMEYDQRFFQSFYAQYQGKMANLAAEQQGPVA
                     VVEVSGDGRL"
     gene            2284..4176
                     /locus_tag="ACZPFK_03245"
     CDS             2284..4176
                     /locus_tag="ACZPFK_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405361.1"
                     /translation="MAGFKQTRLPGASAPSLSKFEIDRFLGADLTNSPANVEEDRSPD
                     CENLIRDVPGKVRKRMGWRTVLRVDGAVNGYHPYKDAPPLLHAGSKLYTLSRDEAGAW
                     AATETAEGLADRRSRSWLLGDKLAIADGHRLLLYDGERVKPASEGAYIPIVSISKAPG
                     AGGQEYEDLNLIQPKFTEQFLGTATDKVYHLTFAPLDETAVEAELLQSDGSWKTLAEG
                     SGFSVNREAGLVTFTTAPGASPVSGQDNVKITAARTVEGYAERIGHCDTGILFGVNGA
                     SDRLFLSGNPELPNWDWYSAQNDPGYWPDGAYSVLGQADSAIVGYSIVSSRLAAHKDS
                     RENDRSVVVRQGDLVENKPAFPIVNTLQGEGAAGKWAFGYLANEPLFLTRLGVYAVTS
                     QDITGEKVANLRSFYLNGRLLAEAGLEEAFAFVYRDMYWLCLNGRAYILDGLQATQSD
                     RSAPYSTRQYAGFYCTGIPARVLWEQEGALWFGTADGRVCRFASDAAALDSYNDDGAA
                     IAARWRTPDLFGQNFYRNKTFSRFYVELASRVATGVRAKARVGGVWEELFADFTTARY
                     FSYSHLTYSKFTYSCDDTPRTLGDKVRIKKVDKAGFLVENSELNEPFGLDAIALEFVE
                     TGYYRV"
     gene            4427..>5676
                     /locus_tag="ACZPFK_03250"
     CDS             4427..>5676
                     /locus_tag="ACZPFK_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like domain-containing protein"
                     /protein_id="HIR1405362.1"
                     /translation="MSFEKITEAELSGKGSVGRPDTPGVTAAEMQRILDEIPREVIVP
                     RFNGLIDALEGLEIEARVKSGDVKAIRVNADNQLEVSADGEHFEATGSSGHLVVGPDG
                     TRLPQRGRLRFTGETVVTDSPDTNETCVTGVKGDKGDKGDKGDQGIQGVQGLRGMVFT
                     PFVDALGNISWELQDAETPAPPAARNIRGPEGPQGIQGPQGATGVQGVQGPAGPQGVK
                     GDTGAAGPQGTKGDTGAAGPQGLPGSQIYHVTAAPQGTLGGLGDWAVNTDSGDWYERT
                     AAGWVKRGCLVGPMGPQGPAGAAGAKGEAGSQGERGPTGPEGPRGPQGEKGDTGAQGP
                     AGPAGAQGPMGPQGAKGDDGADGRSFTVLDLYPTLLALKAAHPVGAAGEAYAVGTVAD
                     NDIYIWGTDTGAWASIGTMQGPMGP"
BASE COUNT         1173 a         1539 c         2190 g          764 t
ORIGIN      
        1 ataagaccgg ggggagaaga gaccatagcc ggtcacgaaa gcggaagtag atgctgagga
       61 gaacggcctt gccgttctca catggacggc cacaaaatca gaggttatga gctgacttac
      121 ggccgtccta atgaccggcg ccacaagcat gaaagttggg gcgcttttgc tgccgtaaaa
      181 gaataagacc agtggggcga gaaagccata gccggtcacg gaagcggaag cgtgtaccaa
      241 ggagaacggc cccgccgttc tcacatggac ggccataaaa tcagaggtta tgagctaact
      301 tacggccgtc ctaatgaccg gcgccaccac aatgaaagtg ggcgcgcttt tgctgccgta
      361 aagaaataag accggtggga gaagagacca tagccggtca cgaaagcgga agtagatgct
      421 gaggagaacg gccctgccgt tctcgcatgg acggccacga aaaccaaagg ttagggcatt
      481 gttaatagcc gtcctaatgc ggaagtgagt gctggggaga acggccctgc cgttctcgca
      541 tggacggcca cgaaaaccaa aggttagggc attgttaata gccgtcctaa ggggggcgca
      601 agcccccctc accatgggca gagaccaggc cgggggcagt acccggcccc atgaaaaaaa
      661 cagccgcccc ggcgggccgc gggcggggag ggggagcagg ccggatgcaa aagaaaaaaa
      721 gcgccgccca gctggcggnn nnnnnnnncg ctgtggaagc aagaggacga cgaacagacc
      781 caggcccaca agcgggccta tggcaactat ctgaaaaata tccgccccgc ggcgcaaagc
      841 cccgcagcgg aaacggcagt tgtaaaagat gcgggtctcg cggccgcaat aagcggtggg
      901 cagggcgccg atttgctcgg cagcaccgcg aaagggctgg gcatcaccgc cggggccacc
      961 gcggcgacag gcgccgccgg gccgtctctc gcggcagggc tcaacgcggc ggaagcttat
     1021 tttctggcct atgaggacgc ggtgcgccaa gggctgccgg tggaggaggc gaaaaagaaa
     1081 gcctgggaaa atgtggtttt aagcatgggg gcggaccagc tggtcggcag cttcggcgaa
     1141 tattttgatt ggggcgatgt gagcggggag gggatcaaca cgctcctcca caagcttttg
     1201 gacctggccc tgagctcttc agcgaaaggc gacgctgagg atgaggataa aaccgacaaa
     1261 gcagcgcagc cgggcttttg aaaagtcttt ccagctctgg atggacagca gccgaaaggg
     1321 tatgaagccc gcgacggccg ggctgccgac caggctgcgc agcagaaata tggagaccgt
     1381 gcgcggagag ggcgcgggca gcccctcgca gaaataaatg gagagcgcgg cgagcagggt
     1441 gcacagggcc acgttgacgc ccgccagcag gtcccgccgc cgaaaacacc tctccggccc
     1501 ccaaaacagg cggcaaaaaa cggggaaaat gaggtcgccg aaagcgaaca gaagagtaca
     1561 gacgacgaga agcacaggaa tggagctgga attgcggcgc tgaacgccgc gcagaagggg
     1621 tgccgcggcg gtcacctcgt tttcgaaatg gggttgagag aacgcgcggc caaagcgccg
     1681 ggcaaaagag gcggcgcggc cgcgcagagc aaagcggcct ttaccaagag aacaacatgc
     1741 ggcggcataa aagaaaagcg ccgccgcgca tgggagggga aaatatgacc ttgggtcagg
     1801 caaaggagcg gctgaagcag ctgctggacg agagcaagcc gaaagcggac ctgacgggga
     1861 agctggaccg gtttttcgac atggggcaaa aagaggtggc gctgtactac cccatctacc
     1921 gcacgaaaga atatgcggct ggggaggcgc gggcgctgcc gggggacttt ttccggggct
     1981 gccaggtggt ggcggatggg gtgtggcacg cctacgaccc cgcggcggcg ctgccgcagg
     2041 gggcgttcac gctgcggtac gaggcgcggc ccgcggacat cccggagaac gcgccggaca
     2101 gcctggagct ggaggtggcg gaggaggcgg cgtccgccgt gctcttctac gtggcggcgc
     2161 agtgcaacag catggagtat gaccagcggt tctttcaaag cttttacgcc cagtatcagg
     2221 gcaagatggc gaacctggcg gcggagcagc agggcccggt ggcggtggtg gaggtgagcg
     2281 gggatggccg gctttaagca gaccaggctg ccgggcgcgt cggcacccag cctgtcgaag
     2341 ttcgagatcg accgttttct gggcgcggac ctgaccaaca gcccggcgaa cgtggaggag
     2401 gaccgcagcc cggactgcga gaacctgatc cgggacgtgc cgggcaaggt gcgcaagcgg
     2461 atgggctggc gcacggtgct gcgggtggat ggggcggtga acgggtatca cccgtacaag
     2521 gacgcgccgc cgctgctgca cgcggggagc aagctgtaca cgctgtcgcg ggacgaggcg
     2581 ggggcctggg ctgcgacgga gacggcggag ggcctggcgg accggcgcag ccgcagctgg
     2641 ctgctggggg acaagctggc catcgcggac ggacaccggc tgctgctgta cgacggcgag
     2701 agggtgaagc ccgcctcgga gggggcgtat atccccatcg tctcgatcag caaggcgccc
     2761 ggcgcgggcg ggcaggagta tgaggatctg aacctgatcc agccgaaatt caccgagcag
     2821 tttttgggca cggccaccga caaggtgtac cacctgacgt tcgcgccgct ggacgagacg
     2881 gcggtggagg ccgagctatt gcaaagcgac ggcagctgga aaaccctggc cgagggcagc
     2941 ggcttttcgg tgaaccggga ggccgggctg gtgacgttca ccaccgcgcc gggggcctcg
     3001 ccggtctcgg ggcaggacaa cgtgaaaatc acggccgcgc gcacggtgga ggggtacgcc
     3061 gagcggatcg ggcactgcga caccggcatc ctgttcgggg tgaacggggc gtcggaccgg
     3121 ctgtttttgt ccggcaaccc ggagctgccg aactgggact ggtattcggc gcagaacgac
     3181 ccgggctact ggccggacgg ggcgtattcg gtgctggggc aggcggacag cgccatcgtg
     3241 gggtactcga tcgtgtcctc gcggctggcg gcccacaagg acagccggga gaacgaccgc
     3301 agcgtggtgg tgcggcaggg ggacctggtg gagaacaagc cggcgttccc gatcgtgaac
     3361 acgttgcagg gcgagggcgc ggcgggcaag tgggcgttcg ggtacctggc caacgagccg
     3421 ctgtttttga cccggcttgg ggtgtacgca gtgaccagcc aggacatcac gggggagaaa
     3481 gtggcgaacc tgcgcagctt ttacctgaac ggcaggctgc tggcggaggc ggggctggag
     3541 gaggcgttcg cgttcgtcta ccgggacatg tactggctct gcctgaacgg gcgggcgtat
     3601 atcctggacg gcttgcaggc gacccagagc gaccggtctg cgccctactc gacgcggcag
     3661 tacgcaggtt tttactgcac gggcatcccg gcgcgggtgc tgtgggagca ggagggggcg
     3721 ctgtggttcg gcacggcgga cgggcgggtg tgccggtttg cgagcgacgc ggcggcgctg
     3781 gacagctaca acgacgacgg ggcggcgatc gcggcgcgct ggcgcacgcc ggacctgttc
     3841 gggcagaact tttaccgcaa caagacgttc agccggtttt atgtggaact ggccagccgg
     3901 gtggcgacgg gggtgcgggc gaaagcgcgg gtgggcgggg tctgggaaga actgttcgcg
     3961 gacttcacga cggcgcggta tttcagctac agccacctga cctactcgaa gttcacctac
     4021 tcctgtgacg acacgccgcg cacgctgggg gacaaggtgc ggatcaagaa agtggacaag
     4081 gcgggttttc tggtggagaa cagcgagctg aacgagccgt tcggcctgga cgccatcgcg
     4141 ctggaatttg tggaaaccgg gtattaccgg gtgtagcttt ggcgatgaag cggagggaaa
     4201 ataacggacc ttgcgcaagc aaggtcgcag cattggccgc tgagcatgag gttatgacgg
     4261 ggcatgcggc cagcgaggaa aacaggatat tacagagtgg aactccggcg atgaagcgga
     4321 ggaaaaataa cggaccttgc gcaagcaagg tcgcagcatt ggccgctgaa cgtgaggtta
     4381 tgacggggct tgcggccaat gcggtagccc tgaaaggaga gaaaaaatgt cgtttgaaaa
     4441 gattacggaa gcggagctga gcggcaaggg cagcgtggga cggccggaca cgccgggtgt
     4501 aacggcggcg gagatgcagc gcatcctgga cgagattcca cgggaggtga tcgtgcccag
     4561 gttcaacggc ctgatcgacg cgctggaggg gctggagatc gaggcgcggg tgaagagcgg
     4621 ggacgtgaaa gcgatccggg tgaacgcgga taaccagttg gaggtatcgg cggacgggga
     4681 gcattttgag gccacggggt cgtcgggaca tctggtggtg ggcccggacg ggacgcggct
     4741 gccccagcgc ggccgtctgc gctttaccgg cgagacggtg gtgaccgaca gcccggacac
     4801 caacgagacc tgcgtcaccg gcgtcaaggg agacaagggg gataagggag acaagggcga
     4861 ccagggcatt cagggcgtcc aggggctgcg cgggatggtg ttcaccccgt ttgtggacgc
     4921 gctggggaac atctcgtggg agctgcagga cgccgagacg ccggcgccgc cggcggcgcg
     4981 gaacatccgc gggccggagg gcccgcaagg catccagggg ccgcaggggg ccacgggcgt
     5041 gcagggggtc cagggcccgg caggacccca gggggtcaag ggagacaccg gcgcggcggg
     5101 tccgcagggg acgaaaggag ataccggcgc ggcggggccc cagggcctgc cgggcagtca
     5161 gatttaccac gtgacggccg ccccgcaggg gacgctgggc ggcctggggg actgggcggt
     5221 gaacaccgac agcggcgact ggtacgagag gacggccgcg ggctgggtga agcggggctg
     5281 cttggtaggc ccgatgggcc cgcagggccc ggcgggggcc gctggagcca aaggagaggc
     5341 cgggtcccag ggagagcggg ggccgaccgg gccggagggc cctcggggtc cgcagggcga
     5401 aaagggggac accggggccc agggcccggc gggcccggca ggggctcagg gccccatggg
     5461 gccgcaggga gcgaaagggg atgacggcgc ggacgggcgc agcttcacgg tgctggacct
     5521 gtaccccacg ctgctggcgc tgaaagcggc gcatcccgtg ggcgcggcgg gcgaagccta
     5581 tgcggtgggg accgtggcgg acaatgatat ctacatctgg ggcacggaca cgggcgcgtg
     5641 ggcgagcatc ggcacgatgc agggcccgat gggtcc
//
LOCUS       DBMDYA010000087         5658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3565_length_5658_cov_1.483894, whole genome shotgun sequence.
ACCESSION   DBMDYA010000087 DBMDYA010000000
VERSION     DBMDYA010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_5658_cov_1.483894"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="ACZPFK_03255"
     CDS             complement(<1..1178)
                     /locus_tag="ACZPFK_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1405363.1"
                     /translation="MREFFHSVTLDRDKCKGCINCIKRCPTEAIRVRDGKAAISPERC
                     IDCGECIRICPHHAKQPIYDPLSVIGDYDYPIALPAPSLYAQFNNLEDTDLLLTALRD
                     MGFAEVYEVARAAELVSDATRRYLARPDVQKPVISSACPAVSRLIRVRFPELVPNVLP
                     LVAPVELAARLAKKEAAARTGLPPERIGAIFISPCPAKVTAMRMPLGSEKSEIDAVVA
                     IKDVYPKLLGLMKHAEPQPLSASGRIGMGWAESGGEAAGLISTERYLAADGIENVIKV
                     LEDLEDEKLGELDFVELDACAGGVLTVENPYIAKAKLKLLRRYLPVSRNHLGEELPPE
                     AKWDQAMEYQPVLELGGDTSESFARYSEMQRLLAALPGLDCGSCGAPTCKALAEDIVK
                     G"
     gene            complement(1283..1723)
                     /locus_tag="ACZPFK_03260"
     CDS             complement(1283..1723)
                     /locus_tag="ACZPFK_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1405364.1"
                     /translation="MGETTQTIRLHYDVPGDDFTRAGEASADLKARLKALGFPPDAVR
                     KVAISLYEGEINLAIHAGGGEIEVAVTPQRVDMVLSDQGPGIADIPLAMSEGYSTAPD
                     EVRSLGFGAGMGLPNMKKYTDEFQIESALGEGTRIQMAVRLGPA"
     gene            complement(1725..2072)
                     /locus_tag="ACZPFK_03265"
     CDS             complement(1725..2072)
                     /locus_tag="ACZPFK_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIR1405365.1"
                     /translation="MKISDIIQILDAQLLTPGADLEKEVHTACGSDMMSDVLAFVKDQ
                     SVLITGLNNPQVVRTADMMDMICIVFVRGKRPDAGILALAEERGMAILCTRHTMFTAC
                     GLLYEQGLRGGVG"
     gene            2348..2836
                     /locus_tag="ACZPFK_03270"
     CDS             2348..2836
                     /locus_tag="ACZPFK_03270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR1405366.1"
                     /translation="MARGTTVPFKGTPEQEQQLRAWLAEHKDMPGAAMPALQYAQEVY
                     GYLPIEVQRMVAEGLDMPLEEIYGIATFYAQFSLSPKGQYKISVCLGTACYVKGSGKI
                     YEKLQEKLGIAGGECTPDGKFSLDACRCVGACGLAPVMTVNDDVYGRLTGDELDEILA
                     KY"
     gene            2856..3413
                     /locus_tag="ACZPFK_03275"
     CDS             2856..3413
                     /locus_tag="ACZPFK_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1405367.1"
                     /translation="MQELSMNVLDVAENSVAAGSTLTSITVEIDTAKRLLAVTIADNG
                     KGMDEETVRRVTDPFYTTRTTRKVGLGLPLFREAAEATGGALHIRSQPGRGTTVRATF
                     TLGHIDLAPLGDMTGTVVGLVQCNPKIDFVYRVVADGEEFCMDTREVRGILEGVPLST
                     PEVAVFLRGYLQENTENLLKRSTLI"
     gene            3410..3781
                     /locus_tag="ACZPFK_03280"
     CDS             3410..3781
                     /locus_tag="ACZPFK_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR1405368.1"
                     /translation="MKSLEELAAIRDRMKNIVNTREAGEDNIRIAVGMATCGIAAGAR
                     PVLNAFTEEVARRELSGVTVTQTGCIGICQYEPVVEVFAPGKEKVTYVKMTPEKVARV
                     VGEHIVGGTPVAEYTIGAAQR"
     gene            3794..5584
                     /gene="nuoF"
                     /locus_tag="ACZPFK_03285"
     CDS             3794..5584
                     /gene="nuoF"
                     /locus_tag="ACZPFK_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR1405369.1"
                     /translation="MFRSHVMVCGGTGCTSSGSAKIMEAFEREVALNGLDKEVKVIHT
                     GCFGLCALGPIVVVYPEGCFYSRVREEDVGEIVREHLLKGRPVTRLLYDETVEDNSIK
                     SLSETNFYKKQHRVALRNCGVINPENIDEYIAFDGYQALGKALTTMTPDEVIQTILDS
                     GLRGRGGAGFPTGLKWKFASGNRGNVQKYVCCNADEGDPGAFMDRSVLEGDPHVVLEA
                     MSIAAYAIGATQGYIYIRAEYPIAVERLRIAIKQAREYGLLGKNIFGTDFCFDIDLKL
                     GAGAFVCGEETALMTSIEGKRGEPRPRPPFPAVKGLFASPTILNNVETYANVPQIILN
                     GPEWFSAMGTEKSKGTKVFALGGKINNTGLVEVPMGTTLREIVEEIGGGIPNGKKFKA
                     AQTGGPSGGCIPASLIDTPIDYDNLLAIGTMMGSGGLIVMDEDTCMVDIAKFFLEFTV
                     DESCGKCAPCRIGTRRLLELLEKITDGRGEMEDLDKIEELAQFIKENALCGLGQTAPN
                     PVLSTLRYFRDEYVAHIKDKRCPAGVCKALLNYKIDPVKCKGCTLCARTCPAGAISGT
                     VRQPHTIDTAKCVKCGACIEKCKFGAISKG"
BASE COUNT         1038 a         1875 c         1817 g          928 t
ORIGIN      
        1 cccttgacga tatcctccgc cagggcctta caggtgggcg cgccgcagct gccgcagtcc
       61 agcccgggca gggcggccag cagccgctgc atttcggagt agcgggcaaa gctctcgctg
      121 gtgtcgccgc ccagctccag caccggctgg tactccatgg cctggtccca cttggcctcg
      181 gggggcagct cctcccccag atggttgcgg ctcaccggca gatagcggcg cagcagcttc
      241 agcttggcct tggcgatata ggggttctcc acggtgagca ccccgcccgc gcaggcgtcc
      301 agctcgacaa agtccagctc gcccagcttt tcgtcctcca ggtcctcgag caccttgatc
      361 acattctcga tgccgtcggc ggccagatac cgctcggtgg agatgagccc ggcggcctcg
      421 ccgccgctct ccgcccagcc catgccgatg cggccgctgg ccgagagggg ctggggctcg
      481 gcgtgcttca tcagccccag cagcttgggg tagacgtcct tgatggccac caccgcgtcg
      541 atctcgctct tttcgctgcc cagcggcatc cgcatggcgg tcaccttggc cgggcagggg
      601 ctgatgaaga tggcgccgat ccgctccggc ggcaaaccgg tgcgggccgc ggcctctttt
      661 ttcgccagcc gcgccgccag ctccaccggg gccaccagcg gcagcacgtt gggcaccagc
      721 tcggggaagc gcacccggat cagccgcgag accgccgggc aggcggagct gatgaccggc
      781 ttttgcacgt ccggccgggc gagatagcgc cgggtcgcgt cgctgaccag ctcggccgcc
      841 cgggccacct cgtacacctc ggcgaagccc atgtcccgca gggcggtcag cagcaggtcg
      901 gtgtcctcca aattgttgaa ctgggcgtag aggctgggcg cgggcagggc gatggggtaa
      961 tcgtaatcgc cgatgaccga cagcgggtcg tagatgggct gcttggcgtg gtgcgggcag
     1021 atgcggatgc actcgccgca gtcgatgcag cgctcagggc tgatagcggc cttgccgtcc
     1081 cgcacccgga tcgcctcggt ggggcagcgc ttgatgcagt tgatacatcc cttgcacttg
     1141 tcccggtcca gtgtgaccga atggaaaaac tcgcgcacgg acaaaacctc ctcgctctgg
     1201 gcggaaagcg cccttttccc cctgggcccg gcccgcagcc gtcagggggg caaaaaacgc
     1261 cgggtccgcc ccggcgctcc cgctaggccg ggcccaggcg caccgccatc tggatgcggg
     1321 tgccctcgcc cagggcggac tcgatctgaa actcgtcggt atattttttc atgttgggca
     1381 ggcccatgcc ggcgccgaag cccaggctgc gcacctcgtc gggcgcggtg gaatacccct
     1441 cgctcatggc cagcgggata tccgcgatgc ccggcccctg gtcggacagc accatgtcca
     1501 cccgctgcgg ggtgacggcc acctcgatct cgccgccgcc cgcatggatg gccaggttga
     1561 tctcgccctc gtacagcgag atggccacct tgcgcaccgc gtcgggcggg aagcccagcg
     1621 ccttgagccg cgctttcagg tcggcgctgg cctcccccgc gcgggtgaaa tcgtcccccg
     1681 gcacatcgta gtgcaggcgg atcgtctgcg tcgtctcgcc catctcagcc cacaccgccc
     1741 ctcagccctt gttcatacag cagcccgcag gcggtgaaca tggtgtggcg ggtgcagagg
     1801 atggccatcc cccgctcctc cgccagcgcc aaaatgcccg catcgggccg tttgccgcgc
     1861 acaaagacga tgcagatcat gtccatcatg tccgccgtgc gcaccacctg ggggttgttg
     1921 aggccggtaa tcagcaccga ttggtccttg acgaacgcca gcacgtcgct catcatgtcg
     1981 ctgccgcagg cggtgtggac ctctttttcc aggtccgccc ccggtgtcag cagctgtgcg
     2041 tccaggatct ggatgatatc cgaaattttc ataaaaaacc tccctgcggc gtggggccgt
     2101 ggccgaactg cgctctttgg cggacagacc gcctctctgc tgtttattat atcggataac
     2161 catgattcgc gcaatgcaca aaaaatcttg cgatctttgt gcaacttttc gacaatttcc
     2221 agtaaaaaag aaatggcggc ctcctgttgg ttttggtata atgaatacgt ctggaatttt
     2281 cgcgtttttg cgaaatgcaa taaagccgcg gctgggtttg cgcggctaaa tagggaggac
     2341 tttttgtatg gcgcgtggaa caacagtgcc cttcaagggc acgccggaac aggagcagca
     2401 gctcagggcc tggctggcgg agcacaagga catgcccggg gcggcgatgc cggccctgca
     2461 gtatgcgcag gaggtctacg gctacctgcc catcgaggtg cagcggatgg tggccgaggg
     2521 cctggacatg ccgctggagg agatctacgg catcgcgacc ttttacgccc agttcagcct
     2581 gagccccaag gggcagtaca aaatctcggt ctgcctgggc accgcctgct acgtcaaggg
     2641 cagcggcaag atctatgaaa agctgcagga gaagctgggc atcgccggcg gcgagtgcac
     2701 gccggacggc aagttcagcc tggacgcctg ccgctgcgtg ggcgcctgcg gcctggcccc
     2761 ggtcatgacc gtcaacgacg acgtctacgg ccgcctgacc ggcgacgagc tggacgagat
     2821 cctggccaag tactgagggc ggggagcgag agggcatgca ggagctttcg atgaacgtgc
     2881 tggacgtggc tgagaacagc gtggccgcgg gcagcaccct cacctcgatc acggtcgaga
     2941 tcgacacggc aaagcggctg ctcgccgtca ccatcgccga caacggcaag gggatggacg
     3001 aggagacggt gcgccgggtg accgacccgt tttacaccac ccgcaccacc cgcaaggtgg
     3061 gcctggggct gccccttttt cgcgaggcgg ccgaggccac ggggggcgcg ctgcacatcc
     3121 ggtcgcagcc gggcaggggc accaccgtga gggcgacttt cacgctgggg cacatcgacc
     3181 ttgcgccgct gggcgacatg accggcaccg tggtggggct ggtgcagtgc aacccaaaga
     3241 tcgactttgt ctaccgggtg gtcgcggacg gcgaggagtt ctgcatggac acccgcgaag
     3301 tgcggggcat cctggagggc gtcccgctct ccaccccgga ggtggccgtg ttcttgcgcg
     3361 gctacctaca agagaatacc gaaaatctgc tgaaaaggag cacactgata tgaagagcct
     3421 ggaagagctt gccgccatcc gcgaccggat gaagaacatc gtcaacacgc gcgaagccgg
     3481 cgaggacaat atccgcatcg ccgtgggcat ggccacctgc ggcatcgccg ccggcgcccg
     3541 cccggtgctg aacgccttta ccgaggaggt cgcccgccgc gaactgtccg gcgtgaccgt
     3601 cacccagacc ggctgcatcg gcatctgcca gtacgagccg gtggtcgagg tgttcgcgcc
     3661 cggcaaagag aaggtcacct atgtgaaaat gacgcccgaa aaggtggcgc gcgtggtcgg
     3721 cgagcacatc gtcggcggca cccccgtcgc cgagtacacc atcggcgcgg cccagagata
     3781 aggaggaaaa gagatgttta gaagccatgt aatggtttgc ggcggcaccg gctgcacctc
     3841 ctccggcagc gccaagatca tggaggcctt tgagcgcgag gtggcgctga acggcctgga
     3901 caaagaggtc aaggtcatac ataccggctg ctttggcctg tgcgccctgg gccccatcgt
     3961 cgtcgtctac cccgagggct gcttttacag ccgcgtgagg gaagaggacg tgggcgagat
     4021 cgtgcgcgag cacctgctca agggccgccc ggtcacccgg ctgctctatg acgagaccgt
     4081 cgaggacaac agcatcaagt ccctgtccga gaccaatttc tacaaaaagc agcaccgcgt
     4141 ggcgctgcgc aactgcggcg tcatcaaccc ggagaacatc gacgagtaca tcgcgttcga
     4201 cggctatcag gcgctgggca aggccctgac caccatgacc ccggacgagg tcatccagac
     4261 catcctggac agcggcctgc gcggccgcgg cggcgcgggc ttccccaccg gcctgaagtg
     4321 gaaattcgcc tccggcaacc gcggcaacgt gcagaaatac gtctgctgca acgccgacga
     4381 gggcgacccc ggcgccttta tggaccgctc ggtgctggag ggcgaccccc atgtggtgct
     4441 ggaggccatg tccatcgccg cctacgccat cggcgccacc cagggctaca tctacatccg
     4501 cgccgagtac cccatcgccg tcgagcgcct gcgcatcgcc atcaagcagg cccgcgagta
     4561 cggcctgctg ggcaaaaaca tcttcggcac cgacttctgc tttgacatcg acctcaagct
     4621 gggcgcaggc gctttcgtct gcggcgagga gaccgcgctg atgacctcca tcgagggcaa
     4681 gcgcggcgag ccgcgccccc gcccgccgtt cccggcggtc aagggcctgt tcgccagccc
     4741 gacgatcctg aacaacgtcg agacctacgc caacgtgccc cagatcatcc tcaacggccc
     4801 cgagtggttc tccgccatgg gcaccgagaa gagcaagggc accaaggtct ttgccctggg
     4861 cggcaagatc aacaacaccg gcctggtgga ggtgcccatg ggcaccaccc tgcgcgagat
     4921 cgtcgaggag atcggcggcg gcatccccaa cggcaaaaag ttcaaggccg cccagaccgg
     4981 cggcccctcc ggcggctgca tcccggccag cctgatcgat acccccatcg actatgacaa
     5041 cctgctggcc atcggcacca tgatgggctc cggcggcctg atcgtcatgg acgaggacac
     5101 ctgcatggtg gacatcgcca agttcttttt ggagttcacg gtggatgagt cctgcggcaa
     5161 atgcgcaccc tgccgcatcg gcacccgccg cctgctggag ctgctggaga agatcaccga
     5221 cggccgcggc gagatggagg acctggacaa gatcgaagag ctggcccagt tcatcaagga
     5281 gaacgccctg tgcggcctgg gccagaccgc cccgaacccc gtcctctcca ccctgcgcta
     5341 tttccgcgac gagtacgttg cccacatcaa agacaagcgc tgcccggcgg gcgtgtgcaa
     5401 ggcgctgctg aactacaaga tcgaccccgt caagtgcaag ggctgcacgc tgtgcgcgcg
     5461 cacctgcccg gccggcgcta tcagcggcac cgtgcgccag ccgcatacca tcgataccgc
     5521 caagtgtgtt aagtgcggcg cctgcatcga gaagtgcaaa ttcggcgcca tcagcaaagg
     5581 ttaagagagg gagtgacaac gaatggaaac cgtacatatc aagatcaata acgtgcccta
     5641 cgaagtcgct gccggctc
//
LOCUS       DBMDYA010000088         5640 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3574_length_5640_cov_1.556599, whole genome shotgun sequence.
ACCESSION   DBMDYA010000088 DBMDYA010000000
VERSION     DBMDYA010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_5640_cov_1.556599"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(214..411)
                     /gene="ssrS"
                     /locus_tag="ACZPFK_03290"
     ncRNA           complement(214..411)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPFK_03290"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(423..743)
                     /locus_tag="ACZPFK_03295"
     CDS             complement(423..743)
                     /locus_tag="ACZPFK_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1405370.1"
                     /translation="MEDNAPDILTLEDEAGQEHVFEVIDAVDFDGRRYLAVVPYIETE
                     EEAEAALAEDADLIIMRVGEENGEEFLDIVEDDEEFYNVGEVFAKRLSELYDIEGEDG
                     GPAS"
     gene            1045..1776
                     /locus_tag="ACZPFK_03300"
     CDS             1045..1776
                     /locus_tag="ACZPFK_03300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1405371.1"
                     /translation="MFRKAFSILCLTVVAGRLLCGCGGAASSSPAKVESSELQFTSPQ
                     AGDTVAVIDTSMGTIRAVLFPSIAPRAVENFVAHAQEGYYDGITFHRVVKDFVIQGGD
                     PTGTGMGGESIWGEPFADEFSDALHNYTGALSMANSGENTNGSQFFIVATPADSVSAK
                     LAGQMEDAGWRQEVISAYQQAGGAPYLDHKHTVFGQVYSGMDVVEKIAGVDVEGENDK
                     PKKDVVINSVTISTYEDTDASSAVD"
     gene            2095..3633
                     /gene="cls"
                     /locus_tag="ACZPFK_03305"
     CDS             2095..3633
                     /gene="cls"
                     /locus_tag="ACZPFK_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1405372.1"
                     /translation="MKRFLKILTSRSLLFALLALLQIGAFFFLVWQFSNIGPYTYAIM
                     IILSVLVLVFVFEKDDLNPSYKVMWVIITVVLPITGAVMYLLWGNQRLSPKTARTFAK
                     IEEQSARAMVPQPLLFEVLEKADVTLSRQAEYLYRNAAAPLYCDTACRYFASGEEFFA
                     YFLPELEKARRCIFMEYFILEPGKMWDATLEVLRRKAEEGVDVRVIWDGFGSLFTLPE
                     DYEQTLRSYGIKAEVFNPLHLTAHISQYIFLNHRDHRKICVIDGEVGFTGGLNFADEY
                     INAKDRCGYWKDTAFVLKGPAVYSLTVTFFKMWSFVRGEVPDYASYLPSRTHPTDGFV
                     QPYADTPLDSENVSESAYLNIINRAQRYVYITSPYLVIDYELQSALSLAAKSGVDVRI
                     VTPGIPDKPYVYILTRSYYRQLLLAGVRIYEYTPGFVHAKMYVSDDAVAVVGSANMDF
                     RSLYLHFENCCAFYGGDIVQDVRADIENCFAVGREVTLEEVEATSWHRRLLQILFRFF
                     APLM"
     gene            3670..4086
                     /locus_tag="ACZPFK_03310"
     CDS             3670..4086
                     /locus_tag="ACZPFK_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HIR1405373.1"
                     /translation="MKTLLVIDGQGGGFGRALIERLRAESLPVRLHAVGTNSLATSAM
                     LKAGADTGATGENAILVGCRRADLIAGPIGIVMANSMMGEFSPAMASAVAESTAARVL
                     IPVNRCDTYVAGATELTFGQSLDRAVELLRRLAAEG"
     gene            complement(4822..5313)
                     /locus_tag="ACZPFK_03315"
     CDS             complement(4822..5313)
                     /locus_tag="ACZPFK_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1405374.1"
                     /translation="MTQKLTIRPAQAADVGVVLGFIRALASYEKMEDQVVATEEGLRE
                     AMFGRGRAQALIAEEDGVPVGFALYFYNFSTFLGHEGLYLEDLFVLPEHRGKGCGKAL
                     FRALAKIAKEQGCGRFEWWCLNWNTPSIGFYRSLGAVPMDEWTVYRLTGPEIAALAEG
                     AGE"
BASE COUNT         1018 a         1854 c         1627 g         1141 t
ORIGIN      
        1 tattttgttt tctctggttg ggccgtgcga tccctgcggg cagccattcc gcttcccccc
       61 tctctgcgct tttgggccgg gtccgggccg gccaaaaagg cgctttatcc ttttgagtgt
      121 aacacgcatc cgggcggcgc acaagccccc caaagcgcgc cggggccggc acaaaacgaa
      181 agcggagagg ttgccctccc cgcttttctt ctcaaaagcc ctgccctgcc gtctgcggcc
      241 aagttaaaac ccgtctctca atctgacagg tgggtgcccc gcctgcgggt tcacgctccc
      301 ttcctcctgc aaagcaggga ggtctgcaat ccgccgccgg acaaaagctc ccgtttaact
      361 taacttgtcg gattataaaa ggctgcaaca aatcgcacag cgcaggggct ctgaaacttt
      421 tttcaggacg ctgggccgcc gtcctcgccc tcgatgtcat agagctcgct caggcgcttg
      481 gcaaagacct cgcccacgtt gtagaactcc tcgtcgtcct cgacgatgtc caaaaactct
      541 tcgccgttct cttcaccgac ccgcatgatg atgaggtccg cgtcctccgc gagggcggcc
      601 tcggcctcct cctcggtctc gatatagggc accactgcga gataccgccg gccatcaaaa
      661 tcaaccgcgt cgatcacctc gaacacatgc tcctgtcccg cttcgtcctc cagggtcagg
      721 atatcgggcg cgttgtcttc catcaggttc cgcctttccc ggcgccgctt gcaggcgggc
      781 cgcaatccag agatgagaag ggacatgcgc cttcttcctc taaaaggata gcccaaaccg
      841 ggggccgatg tcaaggggaa atacccgctt attttgaccc ggcacggcgt agttatccgc
      901 gcacggcccg cattcgaaaa attaacaggc agttcacttt tttcgggtat ccttatacta
      961 taataaatcg cgtagcggct gttttttcag ccctgcggtc cagccccggc tgcggccgca
     1021 gcattgcttt ttggaggcgg tcatttgttc agaaaagcgt tttccatcct ttgcctcacc
     1081 gtcgtcgccg ggaggctttt gtgcggctgc ggcggcgcgg cgtcctcctc ccccgccaag
     1141 gtcgaaagca gcgagctgca gttcacctcc ccccaggccg gcgacacggt ggcggtcatc
     1201 gacaccagca tgggcacgat ccgggcggtc cttttcccct ccatcgcgcc ccgggcggtg
     1261 gaaaattttg tcgctcacgc gcaggagggc tattacgacg gcatcacctt tcaccgggtg
     1321 gtcaaggatt ttgtcatcca gggcggcgac cccaccggca ccggcatggg cggcgagagc
     1381 atctggggcg aaccctttgc ggacgagttc tccgatgcgc tgcacaacta caccggcgcc
     1441 ctctcgatgg caaacagcgg tgaaaacacc aacggcagcc agtttttcat tgtggcgacc
     1501 cccgccgatt ccgtctccgc caagctggcg ggccagatgg aggatgcggg ctggcggcag
     1561 gaggtcatca gcgcctatca acaggcgggc ggcgcgccct acctggacca taagcacacg
     1621 gtgttcggcc aggtgtacag cggcatggac gtggtagaaa agattgccgg cgtggatgtg
     1681 gagggcgaaa acgacaagcc caaaaaagac gttgtgatca acagcgtcac catctccacc
     1741 tatgaggaca cggacgcctc ctccgccgtg gattgagcgc agaactttat tttgagggcc
     1801 acgttttacg gacaggcccc tattgtgcaa aaaaagcagc cgcattgcgg ctgctttttt
     1861 gcttcaaaac tggatgcccg gccgcggctc cgtgccccgg ccgggcattt tctgtacaaa
     1921 tatggggcat catggtaaaa taaccgcaga gcggttattt tacacctatt tcaatggcgc
     1981 atggtctcgc ccctcgcggg gcaaccgacc atcttgcgca cttttgccgt ccgcgctcac
     2041 gcgctcacat gctcgcggcg accgtatttt tcctgttgaa aggggacatg agtgttgaaa
     2101 cgttttttga agatcctgac cagccgcagc ctgctcttcg cgctgctggc cctgctgcag
     2161 atcggggctt ttttcttttt ggtgtggcag ttcagcaata tcggccccta tacctatgcc
     2221 atcatgatca tcctcagcgt gctggtactg gtcttcgtgt ttgaaaagga cgatctcaac
     2281 ccctcttata aagtgatgtg ggtcatcatc accgtggtgc tgcccatcac cggcgcggtg
     2341 atgtatctgc tgtggggcaa tcagcgcctg tcgcccaaga ccgcccgtac ctttgccaag
     2401 atagaggagc agtcggcgcg ggccatggtg ccccagcccc tgctgttcga ggtgttggaa
     2461 aaggcggacg tgaccctgtc ccgccaggcg gagtatctct accgcaacgc ggcggccccc
     2521 ctttactgcg acaccgcctg ccgctatttt gccagcgggg aggagttttt cgcctatttt
     2581 ctgcccgagc tggaaaaggc ccggcgctgc atctttatgg aatatttcat cctggagccg
     2641 gggaaaatgt gggacgccac gctggaggtg ctgcgccgca aggcggagga gggcgtggac
     2701 gtgcgggtga tctgggacgg cttcggcagc ctgttcaccc tgccggagga ttacgagcag
     2761 acgctgcgct cctacggcat caaggcggag gtcttcaacc cgcttcacct gaccgcccac
     2821 atcagccaat acatctttct caaccaccgg gaccaccgca agatctgcgt catcgacggt
     2881 gaggtgggct ttaccggggg gctcaatttc gccgacgaat acatcaacgc caaggaccgc
     2941 tgcggctatt ggaaagatac cgcttttgtg ctcaagggcc cggcggtgta ttcgctcacc
     3001 gtcacttttt tcaagatgtg gagctttgtg cgcggcgagg tgcccgacta cgcctcttat
     3061 ctgcccagcc gcacccaccc caccgacggc tttgtacagc cctatgcgga caccccgctg
     3121 gatagcgaga acgtttccga atccgcctat ctcaacatca tcaaccgggc ccagcgctat
     3181 gtgtacatca cctcgcccta tctggtgatc gactatgagc tgcagtccgc tctctccctc
     3241 gcggccaaga gcggcgtcga tgtgcgcatc gtcacccccg gcatcccgga caagccctat
     3301 gtctacatcc tcacccgcag ctattaccgg cagctgctgt tggccggggt gcgcatctac
     3361 gagtataccc cgggcttcgt acacgcaaaa atgtacgtca gcgacgacgc cgtggcggtg
     3421 gtgggcagcg ccaatatgga ctttcgcagc ctgtacctgc actttgagaa ctgctgcgcc
     3481 ttttacggcg gcgacatcgt acaggacgtg cgcgccgaca tcgagaactg ctttgcagtc
     3541 gggcgcgagg tcacgctgga ggaggtggag gctacctcct ggcatcgccg cctgctgcag
     3601 atcctgttcc gtttttttgc cccgctgatg tgagggcgat ccccccaaaa agaaaggatg
     3661 ttccccctga tgaaaacgct tctcgtcatc gacggccagg gcggcggctt cggccgcgcc
     3721 ctgatcgagc gcctgcgcgc agaaagcctg cccgtccggc ttcacgccgt gggcaccaat
     3781 tccctggcta cctcggccat gctgaaagcc ggggcggata ccggggccac cggtgaaaac
     3841 gccattttgg tgggctgccg gcgcgcggac ctcatcgcgg gccccatcgg catcgtgatg
     3901 gccaacagca tgatgggcga attttccccc gccatggcct ccgcggtggc ggagagcacc
     3961 gccgcccggg tgctgatccc ggtcaaccgc tgcgacacct atgtggccgg cgccaccgag
     4021 ctgacgttcg gccagagcct ggatcgggcg gtggagctgc tgcgccgcct ggcggcggag
     4081 ggctgacgag ccgctctctt tcgccccggt acagaaaagc tgctcttcaa gggcacgttt
     4141 ccgaaagaaa gcagtgcctc ggagagggag attggcgcgc cgggcgccgc caacttcact
     4201 cggcaaccac ccgggggctg cgtgaatttc cccgactctg ccgcgcagct tacccgccca
     4261 aggggaccgg gcagttttga actgagggct ttgaaaaagg caccgttcca aaaaggttgg
     4321 caatctgcag aggttgccaa cctttttgca gatacggagc gccagggccg ccggtccaaa
     4381 accagtattc tctcacctca acgcgcttca agcgggcggc tgccttaaac cccctacggc
     4441 cgccgcccgc tctgccgcac acgccaaaac agggagaaaa cgcgggtgat gcccgcgctt
     4501 tctccctgtt tttttattgc tccgcaataa aaaacaactt attttaatgg ccgtggcccg
     4561 gggacgggcc acatggccga aaaaatagcc tgcggctatt ttttatccgt ttcttcccgc
     4621 cgccggtttg ctgccgaaaa ggctgccgcg agcacttttg tccccccgca gccacgtcgc
     4681 ccggggttgc cctcagtggc ggcgcatcgt ccgccgctcc tcgagcgcct tttcagaggc
     4741 ggggcgcagg gggggcgggc cgctttcaaa cgccgcaccg ccgctttcgg cgcagaaaag
     4801 cagcgccgtc tccccctccg ctcactcccc cgcgccctcg gccagcgccg cgatctcggg
     4861 gccggtcagg cgatagacgg tccactcgtc catgggcacg gctcccagcg agcggtaaaa
     4921 gccgatggag ggggtgttcc agttgaggca ccaccactca aagcggccgc agccctgctc
     4981 tttggcgatc tttgccaggg cgcggaacag cgctttgccg cagcccttgc cccggtgctc
     5041 gggcagcacg aacagatcct ccagatagag gccctcgtgg ccgagaaagg tggaaaaatt
     5101 gtagaagtac agcgcaaagc ccaccggcac gccgtcctcc tccgcgatca gcgcctgggc
     5161 gcggccgcgg ccgaacatcg cctcccgcag cccttcctcg gtggccacca cctgatcctc
     5221 catcttttca tacgaggcaa gggcccggat aaacccgagc accaccccca catccgccgc
     5281 ctgtgcaggg cggatcgtca gcttttgtgt cattttttgt tctcctctct ttcctttcca
     5341 gacgcggccc cgtcaagccg ggctgcggcc gcgtcatctc aggccccggc agtgcgcctg
     5401 ggcgccgggc gtcccccccg acaaaatccg tcaggggtcg taggcctctt cgcccgcaat
     5461 aaagaaagcc gccgggggcg ggccggcccg gccccatccc ggcggctttg cagccgcacc
     5521 gcccctaccg ggcgcggtgc acgctcagtt ttgctgtttt ttctggttgg cgcggaacgc
     5581 catatagctc tccaggatca ccgtggcggc caccgcgtcc agcacctcct tgcgcttttt
//
LOCUS       DBMDYA010000089         5628 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3587_length_5628_cov_1.560702, whole genome shotgun sequence.
ACCESSION   DBMDYA010000089 DBMDYA010000000
VERSION     DBMDYA010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_5628_cov_1.560702"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="ACZPFK_03320"
     CDS             complement(<1..197)
                     /locus_tag="ACZPFK_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405375.1"
                     /translation="MFQTERGSYLRASFYTLGCKVNQNETGALQQLFFENGFEIVGPG
                     DAADVYVVNSCTVTSGGDKKSR"
     gene            317..1534
                     /locus_tag="ACZPFK_03325"
     CDS             317..1534
                     /locus_tag="ACZPFK_03325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1405376.1"
                     /translation="MTRFKRMTAIFLLLCLLAAGAGRVFAAGGGEDAQPTAAQPQQAP
                     EGRFAMPSFDVPAKEAILIAQDTGDVLYQKNPDARVPIASITKVMTLLLTFEAIDAGK
                     ISLSDIVPVSEHAYSMGGSQIWLEPGEQFTLDEMIKAICVSSANDAAVAVAEFVGGSE
                     PVFAEMMTKRAQELGMENTSFKNACGLDTEGHFSSARDVSIMSRELLTKHPKITDYTT
                     IWMDSLRGGATQLINTNKLLRNYQGITGLKTGTTGGAGICISASATRDGLSLIAVVLG
                     SSTSEERFAAATALLDYGFANYEVAGMPDLSAQPDSLPVTGGAEQEVGVSYTLPEHLL
                     LKKGEGAALEATVTLPESVAAPVQAGAELGSVRLAVGGEELGIYPITAKSTVEAMTFG
                     IAFGRLWQALTSL"
     gene            1708..3036
                     /locus_tag="ACZPFK_03330"
     CDS             1708..3036
                     /locus_tag="ACZPFK_03330"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1405377.1"
                     /translation="MSVTLSTKHLSGFVRDEEFDYIWPQVELAHRQLTERSGAGNDFL
                     GWVDLPVDYDKAEFDRIKKAAQKIRSDSDVLIVIGIGGSYLGARAAIEGMTSQLYNES
                     ASTKIYFCGNSISSSYLNTVLALCEGKRVSVNVISKSGTTTEPAVAFRVFKDYLEKTV
                     GPEEARRRIYATTDARKGTLKELSDREGYETFVVPDDVGGRYSVLTAVGLLPIAVAGL
                     DIDQLMAGAAAAREKLSVCDKSNDCYRYAAARNILYRKGKAVELMVSYEPAFTMMNEW
                     YKQLFGESEGKDHKGLFPASVVFSTDLHSMGQFIQDGARVMFETVVDVKHPKEELYIE
                     KAAENFDGLNFLAGQNMSNVNRKAMEGTLLAHTDGGVPNLVLELNSLAEFDFGYLIYF
                     FERACAVSGYLMGVNPFDQPGVESYKKNMFALLGKPGYEDAKAALEAKLK"
     gene            3137..3541
                     /locus_tag="ACZPFK_03335"
     CDS             3137..3541
                     /locus_tag="ACZPFK_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405378.1"
                     /translation="MIKLIVGGKGSGKTKKMIEMINESATTTPGNIVCIEKSMKLTYD
                     VSHAVRLIDVDEYKISGYEMLYGFIAGVLAGNYDIVEVYLDGVLKIGNHDLAGLGGLL
                     AKLDAIAGDNIKVVVTVSSDEADLPEDVKKYI"
     gene            3752..4243
                     /locus_tag="ACZPFK_03340"
     CDS             3752..4243
                     /locus_tag="ACZPFK_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014657.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1405379.1"
                     /translation="MLIDIKKLLTGREEHAAGEFEADLSGESFPAGFSLKAPVRIAYD
                     ARRQGDGARLELDILCQAVIQCDRCLEQVERTYHYTPDYLLFEGDWTGEDPELPFTSA
                     GKLDLKELGYDEILLNLPSSLLCRPDCQGLCPVCGKPVSAGCSCRQNVGDDRLSILKQ
                     LLS"
     gene            4274..4456
                     /gene="rpmF"
                     /locus_tag="ACZPFK_03345"
     CDS             4274..4456
                     /gene="rpmF"
                     /locus_tag="ACZPFK_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1405380.1"
                     /translation="MAVPKRKISKARRDKRRSSVWKLEAPVLVKCDQCGELKLPHKVC
                     GNCGYYKGRMIVKKEA"
     gene            4587..>5628
                     /locus_tag="ACZPFK_03350"
     CDS             4587..>5628
                     /locus_tag="ACZPFK_03350"
                     /inference="COORDINATES: protein motif:HMM:NF039894.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1405381.1"
                     /translation="MAVKVTQVEPGSPAAKAGIRPGDALLQIDGGEINDMLDYEFYTA
                     KREFPVLLERDGAQIRLQVKKEEYEPFGCDFETYLIDKHHSCKNKCIFCFVDQLPRGM
                     RQSLYFKDDDERLSFLFGNYITLTNLSRKELDRIKLMHISPINISVHAVAPELRVRMM
                     KNRFAGDLMPRMRELAAAGIEMNCQLVLCRGVNDGAALQRSMEELTALWPQVRSVAAV
                     PIGVTRYREGLEPLEVYDRQSAARTLDQLVAFGDEMERLHGERLVYPADEWYFLAGRP
                     LPTARFYGDYFQLDNGVGMCRKFYDEFMEELSRPHRVHLPCRADTVCGESVYPLIVSM
                     ADALMKKYRFVRV"
BASE COUNT         1165 a         1634 c         1789 g         1040 t
ORIGIN      
        1 cggctcttct tgtcgccgcc ggaggtgacg gtgcagctgt tgaccacata gacgtcggcc
       61 gcgtcgccgg gaccgacgat ctcgaagccg ttttcaaaaa aaagctgctg cagcgcgccg
      121 gtctcattct ggttcacctt gcagcccagg gtataaaagc tcgctctcaa ataactacct
      181 ctttctgtct gaaacatggt ggtgctttgg ccttatatta tacaaaattt agcgccgcag
      241 cacaaggaga ggtcaaaatt accgcggggg gacatataga ttgggatagg aacacaaaaa
      301 tgggaggttt cggaccatga cccgattcaa aaggatgacc gcgatttttc tgctgctctg
      361 cctgcttgcg gcgggcgccg gcagggtgtt tgccgcaggc ggaggggagg acgcccagcc
      421 caccgccgcc cagccgcagc aggccccgga ggggcgcttt gccatgccct ctttcgatgt
      481 gcccgccaag gaggcgatcc tcatcgccca ggataccggc gacgtgctgt accagaaaaa
      541 cccggatgcg cgggtgccca tcgccagcat caccaaggtc atgaccctgc ttctcacctt
      601 tgaggcgatc gacgcgggca agatcagcct gtcggatatc gtgccggtct ccgagcacgc
      661 ctattccatg ggcggcagcc agatctggct ggagccgggc gagcagttca cgctggacga
      721 gatgatcaag gccatctgcg tgtcctccgc caacgacgcg gcggtggcgg tggccgagtt
      781 cgtcggcggc tcagagccgg tgttcgccga gatgatgacc aagcgcgcgc aggagctggg
      841 catggagaac accagcttca aaaatgcctg cggcctggac accgaggggc atttctccag
      901 cgcgcgggat gtgtccatca tgtcgcggga gctgctgacc aagcacccca agatcaccga
      961 ttacaccacc atctggatgg acagcctgcg gggcggcgcg acccagctga tcaacaccaa
     1021 caagctgctg cgcaactacc agggcatcac cggcctcaag accggcacca ccggcggggc
     1081 gggcatctgc atttcggcca gcgccacccg ggacggcctg tcgctgattg cggtggtgct
     1141 gggcagctcc accagcgagg agcgctttgc ggcggccacg gccctgctgg actacggctt
     1201 tgccaactac gaggtggcgg gtatgcccga cctgtcggcc cagcccgaca gcctgccggt
     1261 caccggcggc gccgagcagg aggtgggcgt cagctacacg ctgcccgagc acctgctgct
     1321 gaaaaagggc gagggagcgg cgctggaggc aacggtcact ttgcccgaga gcgtcgccgc
     1381 gccggtgcag gccggggccg agctgggcag cgtgcggctg gccgtgggcg gcgaagagct
     1441 gggcatttat cccatcacgg ccaaaagcac ggtggaggcc atgaccttcg gcatcgcttt
     1501 cggcaggctc tggcaggctt tgaccagcct gtgacggggc tgtgacggac gggttaattt
     1561 ttcgtggccc gggggttttt aacttgacag ccgcagggga gtaatatatc attaatacaa
     1621 ggaaatcgcg gggaagatat ccccgccgcg ccgccttgaa atacggcgct gacacttccc
     1681 caatgtatac ggttggaggc aattgttatg tccgtaacct tgagtacgaa acacctttcc
     1741 ggctttgtcc gcgacgaaga attcgattat atctggcccc aggtcgagct ggctcacagg
     1801 cagctgaccg agcgcagcgg ggcgggcaac gactttctgg gctgggtcga cctgccggtc
     1861 gattacgaca aggcagagtt tgaccgcatc aagaaagcgg cgcagaagat ccgcagcgac
     1921 agcgacgtgc tgattgtcat cggcatcggc ggcagctatc tgggcgcccg cgccgccatc
     1981 gagggcatga ccagccagct ctacaacgag tcggcgtcca ccaagatcta tttctgcggc
     2041 aacagcatct cctccagcta tctcaacacg gtgctggccc tgtgcgaggg caagcgggtg
     2101 tcggtgaacg tcatctccaa atccggcacc accaccgagc cggccgtggc gttccgcgtg
     2161 ttcaaggact acctcgagaa gacggtcggc cccgaggaag cccgccgccg catctacgcc
     2221 accaccgacg cgcgcaaggg caccctcaaa gagctgtccg accgtgaggg ctacgagacc
     2281 tttgtcgtgc ccgacgacgt gggtggccgc tattcggtgc tcaccgcagt gggcctgctg
     2341 cccatcgccg tagcggggct cgacatcgac cagctgatgg ccggcgcggc cgctgcccgc
     2401 gaaaagctgt cggtctgcga caaatcgaac gattgctacc gctatgccgc ggcccgcaac
     2461 attctgtacc gcaagggcaa ggccgtggag ctgatggtca gctatgaacc ggcgttcacc
     2521 atgatgaacg agtggtataa gcagctcttc ggcgaatccg agggcaagga tcacaagggc
     2581 ttgttcccgg cctcggtggt cttctccacc gatctgcatt ccatgggcca gttcatccag
     2641 gacggcgccc gggtgatgtt tgagaccgtg gtggacgtca agcaccccaa ggaagaattg
     2701 tacattgaaa aagcggctga gaactttgac ggcctgaatt tcctcgccgg gcagaacatg
     2761 tccaacgtga accgcaaggc catggagggg accctgctgg cccacaccga cggcggcgtg
     2821 cccaacctgg tgctggagct caacagcctg gccgagttcg acttcggcta tctgatctat
     2881 ttctttgagc gcgcctgcgc cgtcagcggc tatctgatgg gggtcaaccc gtttgaccag
     2941 ccgggcgtgg agagctacaa aaagaatatg ttcgcgctgc tgggcaagcc cggctacgag
     3001 gacgcaaagg cggccctgga ggcaaagctg aaataaagcg cggccgcctg attaacgggg
     3061 cggacgttca gccagaatca aggtagacaa catcaggcac cgccctgctg atgttcaaaa
     3121 taaaggagga attccaatga taaagcttat tgttggtggc aaaggctccg gcaagaccaa
     3181 aaagatgatc gagatgatca acgaaagcgc caccaccacg ccgggcaata tcgtgtgcat
     3241 tgaaaaatcc atgaagctga cctacgacgt ctcccacgcc gttcgtctga tcgacgtgga
     3301 tgaatataag atctctggct atgagatgct gtacgggttc atcgcgggcg tgctggccgg
     3361 caactacgac atcgttgagg tctacctgga cggcgtgctg aagatcggca accacgacct
     3421 ggccggcctg ggcggcctgc tggcaaagct ggacgccatc gcgggcgaca acatcaaggt
     3481 ggtggtcacc gtctcctccg acgaggcgga cctgcccgag gacgtcaaaa agtatatctg
     3541 atcgcaaagg gacaaaagcg gggcgcgcag ccccgctttt ttgccctgtg cggaccggct
     3601 cgggccgtgc gggggcgcag gggcgcacgg cgatgggcga acggttgaaa atttgccttt
     3661 ttgtgttgac aaaccgcgcg gtaaccctta taataatcaa gcggcctttt gtcaaagggg
     3721 cattttgtgc ccggccgcag cgaggtcttt tatgctcata gacatcaaaa agctcctcac
     3781 cggcagggag gagcacgcgg caggggagtt tgaggccgat ctctcgggcg agagcttccc
     3841 ggcgggtttt tctttgaaag ccccggtgcg catcgcctat gacgcgcggc gccagggcga
     3901 tggggccagg ctcgaattgg acatcctctg ccaggcggtc atacaatgcg atcgctgttt
     3961 ggagcaggtg gagcgcacct atcactacac gccggattat ctgctctttg agggcgattg
     4021 gaccggcgag gatcccgagt tgcctttcac ctctgccggc aagctggatc tgaaagagct
     4081 cggctatgat gaaatcctgt tgaatctgcc gagcagcctg ctgtgcaggc ccgattgtca
     4141 aggattatgc ccggtatgcg gcaagccggt ctcagccgga tgcagctgcc gtcaaaatgt
     4201 cggcgacgac aggctgtcca tattaaaaca actgctgtct taagttttac acatttagag
     4261 gaggtgcttt aaagtggcag tacccaagag aaaaatttcc aaggctagac gggacaagag
     4321 acgctcctct gtgtggaagc tcgaggcccc tgtcctggtg aaatgcgacc agtgcggcga
     4381 gctgaaactc ccccataagg tatgcggcaa ctgcggctac tacaagggta gaatgatcgt
     4441 caaaaaggaa gcgtaagcgc ttcgaagcgc gggggaggaa atgtttcctc ccccgttttt
     4501 catgtcacaa aggcggccac tgcctgagcg gggcgaaagt gcggcatact gaaagcgggg
     4561 cgcgaaaaaa cagggggggg acgcagatgg cggtaaaggt cacgcaggtg gagccgggca
     4621 gcccggcggc aaaggcgggt atccggcccg gggacgcgct gctgcagatc gacggcggcg
     4681 agatcaacga catgctggac tacgagttct ataccgcgaa acgcgagttc ccggtactgc
     4741 tggagcggga cggcgcgcag atcaggctgc aggtcaaaaa agaggagtac gagcctttcg
     4801 gctgcgattt tgagacctat ctgatcgaca agcaccactc ctgcaaaaac aagtgcattt
     4861 tctgctttgt cgaccagctg ccgcggggca tgcggcagag cctctatttc aaagacgacg
     4921 acgagcggct ctctttcttg ttcggcaact acatcaccct gaccaacctc tcccgcaagg
     4981 agctggaccg tatcaagctg atgcacatct cgcccatcaa tatctcggtg cacgcggtgg
     5041 cccctgagtt gagggtgcgc atgatgaaaa accgctttgc cggggatctg atgccccgca
     5101 tgcgggagct ggccgcggcg ggcatcgaga tgaactgcca gctggtgctc tgccgggggg
     5161 tcaacgacgg cgcggccctg cagcgcagta tggaagagct caccgccctg tggccgcagg
     5221 tgcgcagcgt ggcggcggtc cccattgggg tgacccgcta ccgcgaaggg ctggagccgc
     5281 tggaggtcta tgaccggcag tcggctgcgc gcacgctgga tcagctggtg gcctttggcg
     5341 acgagatgga gcggctgcac ggcgagcggc tggtctaccc ggcggatgag tggtattttt
     5401 tggccggccg ccccttgcca acggcgcgct tttacggcga ttattttcag ctggacaacg
     5461 gcgtgggcat gtgccgcaag ttctatgatg agttcatgga agaactgtcc cgcccgcacc
     5521 gcgtccatct gccctgccgg gccgacaccg tctgcggcga gtcggtctac ccgctgatcg
     5581 tctcgatggc ggacgcgctg atgaaaaagt accgctttgt gcgggtgc
//
LOCUS       DBMDYA010000090         5602 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3607_length_5602_cov_1.609162, whole genome shotgun sequence.
ACCESSION   DBMDYA010000090 DBMDYA010000000
VERSION     DBMDYA010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_5602_cov_1.609162"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..1210
                     /locus_tag="ACZPFK_03355"
     CDS             182..1210
                     /locus_tag="ACZPFK_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405382.1"
                     /translation="MTIKDIAREAGVSTATVSRALNSPELVSEKKRKAIEAIVEKYGY
                     TLNAAAREMVTRRTHLIGVVVPDITNTYVATALDSFAAALGGYGYSMFISVSNMKAEL
                     ELDYLDNLKSKRVEAVVLPGNRLLSPEHDRLLAEKLKGTPILRIGNTNCEEFYCADPD
                     EEQGAFQAIEYLIGLGHTRIAFLNGSVTVDSFFHKQQGVERAMKKHGIPIRPEYMPQV
                     DTGNYGYDSYHTTVELLRLPQRPTAIFTAGDQFALGIYKAASVLGYQIPSDLSVVGFS
                     GSPLSESIYPALTTISQYAHETGKQAARYLMEILEGEEETPRRIQLRAELVVRDSCAV
                     PAAEKEER"
     gene            1454..2965
                     /locus_tag="ACZPFK_03360"
     CDS             1454..2965
                     /locus_tag="ACZPFK_03360"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405383.1"
                     /translation="MDDFVLEFREVSKLFPGVVALDKVSFGVKRGAVHVLMGENGAGK
                     STLMKIISGEHEVTSGEILLDGEKMEFKNPQMAREKGIAMIYQELQYVPEFTVEEFLM
                     MGREPGRKANCFIDWKELHRAAADILKQEGLRYDLKTKIKDLSVSDIQLLEITRAIST
                     QARIIIMDEPTSALSNAEVERLFENIRRMKERGITVLYISHKMDEVFRIGDDMTVMRD
                     GKHIATDGIANYDYDKVVRLMVGREISSVYPKEQAEIGDVVLEVKGLTSAMLKVDHVS
                     FQVRRGEILGIAGLMGAGRTELVSMIAGLEKYDEGEIWVEGKPVQIHSVAGAIRNGIM
                     MATEDRRRQGIISCRSIKENISLPNLRRLSRFLFVDQKKENQAVDLIWKRLRIKAPSK
                     QTLVSNLSGGNQQKVVLAKWLLSEPKVLILDEPTRGIDVAAKHEIYKLMNEMVREGIA
                     IIMISSELPEFIGMCDRAYTMYEGRFTGELQRSQLTQEKIMKLATGVSKYEKG"
     gene            2952..3917
                     /locus_tag="ACZPFK_03365"
     CDS             2952..3917
                     /locus_tag="ACZPFK_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405384.1"
                     /translation="MKKVDVRALGATVTRKYGIYLVAVCVVILGSVLSPHFFTVGNFL
                     SVARQISITGILAFAETLIIISGCTDLSLGAQVAFAGVLGMDAYLATGHSFLIAILVA
                     MAVACCCGLFNGIMVTKVGLAPFIATLAMDMIARGSAYLYTGGNPIYQTEGFSKISQA
                     TVLSIPVPVLFLLGVMLVCWILLRTTRYGRQLFAVGGNQEAARASGISIVKTRVIAYL
                     IASAFTGIAAVLQMARINAGMPDTAQGYHGDAIAAAVVGGTAFGGGVGTAQGTLVGAL
                     IIGLINNILNLMGVQSFVQQIVRGVVILLAVGWDIQSKRRKVKRA"
     gene            3959..4981
                     /locus_tag="ACZPFK_03370"
     CDS             3959..4981
                     /locus_tag="ACZPFK_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1405385.1"
                     /translation="MKKNHKFWTAFLAAALTISLFTACGGSGSSASAPSGGSGAVSDA
                     GAEKTYNITYLSKDMAQPYAIWLANSLTQIAEQDYPDFDIIILDQQQDPSKGVAMIEQ
                     AINQQVDGIIIQKAGGYDTDDLFRSVADAGIPLVTVNIPVDDGVSSNVACSNYELGET
                     VGSYAREQIPENAKVLVLRGNPGDLEEERWQGFKESLFDQRPDLEILDTQIAYYDKAK
                     AIAISDDWLQRFPEIDAVVSMNDGQALGFIESCKANGRDLSDIQIYGVDGLADGCLAV
                     EAGEMTATVVQNATEQARHGLRIMHELLTGESEGPMDYIVPAELINKDNIDEWIAFHK
                     EIGMIE"
     gene            5051..>5602
                     /locus_tag="ACZPFK_03375"
     CDS             5051..>5602
                     /locus_tag="ACZPFK_03375"
                     /inference="COORDINATES: protein motif:HMM:NF019845.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR1405386.1"
                     /translation="MEGMMRSVVYDAPGMGSVRLKPIPRVGDGQLLIKIVSCGICKGA
                     DSAHDTVGVGSGLAKYPGVTPGHEFAGDVCEVGKNVTAFKPGDRVTADNTVSCGNCYY
                     CRTLRPNLCDRFGSIGHNIPGGFAEYVLVDEEKAYKIPDALSYNDACFAEPMACCVHA
                     MDRLFALSSGYGEQVIHGMDTAGH"
BASE COUNT         1316 a         1394 c         1731 g         1161 t
ORIGIN      
        1 atcagcctcc tttgacgatg aggggggccc ggtataaaat aggaaagaaa acggaaaaga
       61 ttagaaaaaa gctacaaagg aagactttaa aatatggata taatttttga acaaaacact
      121 tgatttttag gcaaatcaag ttataattgg aaatgtaaag ctttacataa aaggagcccc
      181 catgacgatc aaagacattg cccgcgaggc gggcgtttcc acagcgactg tttcccgggc
      241 gctgaacagc ccggagctcg tttcagaaaa aaagcgcaag gcgatcgagg ccattgtgga
      301 aaagtacgga tacaccttga atgccgccgc gcgggagatg gtcacccgcc gcacccatct
      361 gatcggggtc gttgtaccgg atatcaccaa cacctatgtg gccactgcgc tggacagctt
      421 cgcggcagcc ctgggcggat acggatacag catgttcatt tcagtgagta acatgaaagc
      481 ggaactggag ctcgattacc tggacaatct caagagcaag agagtggagg cagtggtact
      541 gccgggcaat cgccttttgt caccggagca cgaccgcctg cttgccgaga aattgaaagg
      601 gacgccgatc ctgcgcatcg gcaataccaa ctgtgaagag ttctattgtg ccgacccgga
      661 cgaggagcag ggcgcttttc aggcgataga atatttgatc gggctggggc atacccgcat
      721 tgcctttctt aacggcagtg tgacggtgga ctcctttttt cacaagcagc agggggttga
      781 gcgtgcgatg aaaaaacacg gtatccccat tcggccggaa tatatgcccc aggtggacac
      841 gggcaactat ggttatgatt cctaccatac cacggtcgaa ctgctccgcc tgccgcagcg
      901 gcccaccgcc attttcaccg ctggcgacca gtttgcactg ggcatctaca aagcggcgag
      961 tgtactggga tatcagatcc ccagcgacct ctctgtggtg ggcttcagcg gctcccctct
     1021 ttcagagagc atctatccgg cgctgaccac gatctcccaa tacgcacatg agaccggcaa
     1081 gcaggcggcc agatacttga tggagatcct ggagggagag gaagagacgc cgcggcggat
     1141 ccagctgcgg gcggagctgg tcgtgcggga ctcctgcgct gtgccggcgg cagaaaagga
     1201 ggagcgttga cgcccttgcg gattatttta ttgtagttag ttcctttcgg ggaagatgac
     1261 ttggaaaaaa cggttcccat cacatcttgg atgggacgct tttttaccat aaaatgtaaa
     1321 cgcttacatt ttatggctta taactttttc gaatcccctg atagggaaaa ggaggaaagg
     1381 aggacaggaa aaaggcccgc atggcccggt aaagaccacg gaaaagagga tgcgtaagga
     1441 ggcgagtgaa aaaatggacg acttcgtgct ggagtttcgt gaggtctcaa agctttttcc
     1501 cggcgtagtg gcgctggaca aggtgagctt tggggtgaag cgcggcgcgg tccacgtgtt
     1561 gatgggggag aacggcgcag gcaaatcgac gctgatgaag atcatcagcg gcgagcatga
     1621 ggtgaccagc ggggagatcc tgttggatgg ggaaaagatg gagtttaaaa accctcaaat
     1681 ggccagggaa aaagggatcg ctatgatcta tcaagagctt caatatgtcc ccgaattcac
     1741 ggtggaagaa ttcttgatga tggggaggga gccgggcaga aaagcgaatt gcttcatcga
     1801 ctggaaagaa ctgcatcggg ccgccgcaga tattttaaag caagagggcc tgcgctatga
     1861 tctcaaaacc aaaatcaagg atctgagcgt ttcagatatt cagcttttgg aaatcacacg
     1921 cgcgatctcg acccaggcgc gcatcatcat catggacgag cccacctcgg cactgtccaa
     1981 cgcggaggtg gagcggctgt ttgagaatat ccgccgcatg aaagagcgcg ggatcactgt
     2041 gctctatatc tcccataaga tggacgaggt cttccggatc ggggatgata tgacggtgat
     2101 gcgggacggc aagcacatcg ccacggacgg gatcgcaaat tatgattacg acaaagtcgt
     2161 gcggctgatg gtgggacgcg agatctccag tgtctatccc aaagagcagg cagagatcgg
     2221 ggatgtggtg ttggaggtaa aaggcctgac cagcgccatg ctgaaagtgg atcacgtgag
     2281 cttccaggtg cgccgggggg agatcctggg cattgccggg ctgatggggg ccggccgcac
     2341 tgaactggtc tcgatgattg ccggcctgga aaagtatgat gagggcgaga tttgggtgga
     2401 gggcaagccg gtgcagatcc acagtgtggc tggagcgatc cgcaacggca ttatgatggc
     2461 caccgaggac cggcgcagac agggcatcat ctcctgccgc agcatcaaag aaaatatctc
     2521 gctgcccaat ctgcgccggt tgtcccgatt tctgtttgtg gatcagaaaa aggaaaatca
     2581 ggccgtggac ttgatctgga agcgcctgcg catcaaggcc cccagtaaac agaccttggt
     2641 gtccaatctg tccggcggca accagcaaaa ggttgttttg gcaaaatggc tgttgtcgga
     2701 gcctaaagtt ttgattttgg atgaacccac ccgcggcatc gacgtagcag caaagcatga
     2761 aatctacaaa ttgatgaacg agatggtcag agaggggatt gcaatcatca tgatctcctc
     2821 cgaattgccg gaatttatcg gtatgtgcga ccgggcctat acgatgtatg agggtcgttt
     2881 tacaggggag ctgcaacgat cacagctgac gcaggagaag atcatgaaac ttgcaacggg
     2941 ggtgtcaaaa tatgaaaaag gttgatgtgc gtgccttagg agccacggtc acgagaaaat
     3001 atgggatcta tctggtagcg gtctgcgtgg tgattctggg ctcggtgctc agccctcatt
     3061 tttttacggt gggaaacttt ctcagtgtgg cgcgccagat cagcattacc ggcatcctcg
     3121 cttttgccga gacgctgatt atcatcagcg gatgtaccga cctgtctctg ggggcacagg
     3181 tggcctttgc gggtgtgctg ggcatggatg cgtatctggc cacaggtcac agctttttaa
     3241 tcgccatttt ggtggccatg gcggtcgcct gctgctgcgg gctcttcaac ggtatcatgg
     3301 tcaccaaggt ggggcttgcc ccgtttattg cgaccttggc aatggatatg atcgcgcgcg
     3361 ggtcggctta cctctatacc ggcggcaacc ccatctacca gacggaaggc ttttctaaaa
     3421 tttcgcaggc gacagtgctc tccatcccgg tgccggtact ttttttgctg ggagttatgc
     3481 tcgtgtgctg gattttgctg cgcaccaccc gatatgggcg ccagctcttt gcggtgggcg
     3541 gcaaccagga agcggcgcgg gcctctggca tcagcatcgt gaaaacacgt gtgatcgcct
     3601 atttgatcgc atccgctttt accggcatcg ccgcggtcct gcagatggcc cgcatcaacg
     3661 cgggcatgcc cgacaccgcc cagggctatc acggtgatgc catcgcagcg gcggtggtgg
     3721 gcggcacggc gtttggcggc ggcgtgggta ccgcccaggg caccctggtg ggcgcgttga
     3781 tcatcggctt gatcaacaat atcctcaacc tgatgggagt gcagtccttt gtgcagcaga
     3841 tcgtgcgcgg cgttgtcatt ttgcttgcgg tgggctggga tatccagagc aagcgcagaa
     3901 aggtgaagcg ggcgtgaaaa cgcctttggc agagaaaaag aaatatagga ggcaaaagat
     3961 gaaaaagaac cacaagtttt ggacggcttt tctggcagcg gcactgacaa tctctctgtt
     4021 taccgcctgc ggaggctccg gcagttctgc ttcggcgccc tccggcggct ccggcgcagt
     4081 ttcggatgcc ggtgcggaaa aaacctacaa catcacctat ctgtcaaaag atatggcgca
     4141 gccctatgcc atctggctgg cgaattcgct cacccagatt gcggagcagg actatcccga
     4201 ttttgacatc atcattttgg atcagcagca ggacccctct aaaggggtgg cgatgatcga
     4261 gcaggcgatc aaccagcagg tggacgggat catcatccag aaagccgggg gatatgacac
     4321 cgacgattta ttccgcagtg tggcggacgc gggtatccca ctggtcacgg tcaacatccc
     4381 ggtggacgac ggcgtatcca gcaatgtggc ctgcagcaac tatgaactgg gcgagacggt
     4441 gggcagctac gcccgggagc agataccgga aaacgcaaag gtactggtgc tgcgcggcaa
     4501 cccgggggat ttggaggaag agcgctggca gggctttaag gaaagcttgt ttgaccagag
     4561 gccggacctt gaaatcctcg acacgcagat cgcttattat gacaaggcga aagcgatcgc
     4621 tatttccgac gactggctgc agcgcttccc cgagatcgac gcggtggtct cgatgaatga
     4681 cgggcaggcc ttgggtttca tcgagtcctg caaggcgaat ggacgagatc tgagcgatat
     4741 ccagatttat ggtgtggacg gactggcgga cggctgtctg gcagtggaag cgggggaaat
     4801 gaccgccacg gtggtacaga atgccaccga acaggcgcgt catggcctgc gcatcatgca
     4861 tgagctgttg accggggaga gcgagggacc tatggattat atcgtgccgg cggagctcat
     4921 caacaaggac aatatcgacg agtggatcgc gttccacaaa gagatcggca tgatcgaata
     4981 aaaaactgcg ggaagcgcgt ttttcagcgc gtggctttta aaagcgattc ctatgaaagg
     5041 tggaaaagaa atggaaggaa tgatgcggtc ggtggtttac gacgcgccgg gcatgggctc
     5101 ggtgcgtctc aaaccgatcc cccgggtggg ggacggccag ctcttgatca agatcgtctc
     5161 ctgcggtatc tgcaagggcg ccgactcggc ccatgacacg gtgggcgtgg gctcgggcct
     5221 ggcaaaatat ccgggcgtca cgccggggca cgagttcgca ggcgacgtgt gcgaggtggg
     5281 caaaaacgtc acggctttca agcccggcga ccgggtgacc gccgacaaca ccgtgtcctg
     5341 cggcaactgc tattattgcc gcaccctgcg ccccaacctg tgcgaccgat tcggctctat
     5401 cgggcacaac atccccggcg gctttgccga gtatgtcctg gtggacgagg agaaagctta
     5461 taagatcccg gacgctttgt cctacaacga cgcctgcttt gccgagccga tggcctgctg
     5521 tgtccatgcc atggatcgcc tgttcgcgct gagcagcggc tatggggaac aggtgatcca
     5581 tggcatggac acagcaggcc at
//
LOCUS       DBMDYA010000091         5573 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3624_length_5573_cov_1.499452, whole genome shotgun sequence.
ACCESSION   DBMDYA010000091 DBMDYA010000000
VERSION     DBMDYA010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3624_length_5573_cov_1.499452"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="ACZPFK_03380"
     CDS             <1..423
                     /locus_tag="ACZPFK_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1405387.1"
                     /translation="LDVTIQAQVLQMMNDLKTKLDTSMLLITHDLGVVSQTCDKVAVM
                     YAGEVVEYGRAEDIFLGDRHHPYTEGLFGSLPGLNASAKRLSPIDGLMPDPTALPEGC
                     RFHPRCPHCTERCKTQAPAQHLRDGHMIRCHRMEEEVR"
     gene            425..1378
                     /locus_tag="ACZPFK_03385"
     CDS             425..1378
                     /locus_tag="ACZPFK_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405388.1"
                     /translation="MAAPLLEIQNLKKYFSTPAGQLHAVDDVSLTIEAGQTLGVVGES
                     GCGKSTLGRTVLRLLEPTAGKVLYNGEDITRFNKQRMFQARQELQMIFQDPYASLNPR
                     LSVAEIIAEPLIVAKRCRSKGEMNDRVAELMETVGLAPRLMNSYPHELDGGRRQRIGV
                     ARALATDPRFIVCDEPVSALDVSIQAQILNLLMDLQDEKGLSYMFITHDLSVVKHISS
                     HIAVMYLGQCVEYAETQELFAHPAHPYTRALLAAIPVIDITRRGKQLETIQGEVTSPI
                     DPAPGCRFAPRCKYACEGCTAGQMELRELTAGHFVACPYAR"
     gene            1408..2637
                     /locus_tag="ACZPFK_03390"
     CDS             1408..2637
                     /locus_tag="ACZPFK_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014164.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1405389.1"
                     /translation="MSRIRQNYARLVLTRGVNLQPGQILVIDAPVWTSEFVHLVSEEA
                     FRLGAKDVVIHYADPDADRLRLLHADEETLCDVPRWQVESQTLYGEKNACFLRLDSND
                     PDGMAGVDPARLSLWKKAVSAPLEGLRLKRMSNDLAWSGVPAPNARWAKKVFPAMGEP
                     EALEALWQAVYRCCYVSEESAEAGWDAHVEEMQRNVRKINALGVRSLRFQNGKGTDLT
                     LELCDGGVFAGGICHCPEPDGIVFAPNIPTEEILTTPHRDKVNGVVYNTLPLVYGGSI
                     IDDFCLTFRDGVVVDWRCGQGAEVLAGILDTDAGTRRLGEVALVPFDSPINQLGVLFY
                     DTLFDENASCHLALGAGYVDVIPGGDRSKEALVARGLNTSMLHVDFMFGSRDMRCTAR
                     DADGREVEIFRDGLFVI"
     gene            2868..4946
                     /locus_tag="ACZPFK_03395"
     CDS             2868..4946
                     /locus_tag="ACZPFK_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405390.1"
                     /translation="MEKRVSGKTGTGLSCLALLPAVALVWGVAVAGGCAWWALPAALC
                     GAGLCILLPGCALLYALKRLTPLPQGFELPLALLLGSGFFAALTCLCARFGLAWPLRV
                     VPPAVALAGFFAFRRDAGQTAERLRHWRLHRADALLLVALLVVYALCWETATAHPAAV
                     GQTVLKQDFLWNIGNARSFWLGFPPQDIRFAGVRLHYHYLNELLAAGLSAACGASVYD
                     LLGFVWPPLVLAAALAGLRGLGRLFYEEDGEARARRLAAFLPFALFGAGSIDLIWRLF
                     SSSGFETSSLYHYITNVNACGTALLFSSVFLGLVLWAMRRQYRVNAAFFVLTACCTVV
                     LCFAKGPVALILALAFAAALLWQAVQKKARLRGILLAGVVLLTAGGIYFAIFSSGANN
                     MALTPDGTLLKSPLAPLLIALGRRSNFAYYLCLPFFMALHQLCVSPVVTVLYAGCLWR
                     DVRRLRSLDGPRLLLHAGALGGMLAYYLFDHYAMSQIYFLYLSQQCAILLGIELLFSI
                     RRPALKRAAFRLGVFCALSASAAALALCGDGVGLLATRLDDEPCGAQYNVTAGNEAAA
                     QWLGAEMPREAVFATNRYYDVSPEAGNSNLYTALSGRQAYMEGFLYAVSNMGVPRQWV
                     EERFTRNAVLFSPKSDPATVLHSAREAGISYLVFDEAYAFTGENGPQFTALEKVYDQG
                     GVQIYRVPQA"
     misc_binding    5065..5284
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5360..>5573
                     /locus_tag="ACZPFK_03400"
     CDS             5360..>5573
                     /locus_tag="ACZPFK_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1405391.1"
                     /translation="MRELDKQYDPSQVEDSTYRFWLEHKYFHAERDPEKKPYTIVIPP
                     PNITGQLHMGHAMDETLQDILIRTKRM"
BASE COUNT          900 a         1743 c         1909 g         1021 t
ORIGIN      
        1 ctggacgtga ccatccaggc gcaggtactg cagatgatga acgacctgaa gaccaagctg
       61 gacacctcga tgctgctgat cacccatgac ctgggcgtgg tcagccagac ctgcgacaag
      121 gtggcggtga tgtacgccgg cgaggtggtg gagtacggcc gggcggagga tatctttctg
      181 ggcgaccgcc accaccccta cactgagggg ctgttcggct cgctgcccgg cctcaacgcg
      241 tcggcaaagc ggctctcgcc catcgacggg ctgatgccgg accccaccgc gctgccggag
      301 ggctgcaggt tccatccccg ctgcccgcac tgcaccgagc gctgcaagac ccaggccccc
      361 gcgcagcatc tgcgggacgg ccacatgatc cggtgccacc ggatggaaga ggaggtgcgg
      421 tgagatggcg gccccattgc ttgagatcca aaatctgaaa aaatattttt ccacccccgc
      481 gggccagctg cacgccgtgg acgacgtgag cctgaccatt gaggcgggcc agacgctggg
      541 cgtggtgggc gaatccggct gcggcaagtc gacgctgggg cgcaccgtgc tgcgcctgct
      601 ggagcccacg gcgggcaagg tgctgtacaa cggcgaggac atcacccgct tcaacaagca
      661 gcgcatgttc caggcccggc aggagctgca gatgatcttt caggacccct acgcctcgct
      721 gaacccccgc ctgtcggtgg cggagatcat cgccgagccg ctgatcgtgg cgaagcgctg
      781 ccgcagcaag ggcgagatga acgatcgggt ggccgagctg atggagaccg tgggcctggc
      841 gccccggctg atgaacagct acccgcacga gctggacggc ggccgccgcc agcgcatcgg
      901 cgtggcccgc gcgctggcca ccgacccccg ctttatcgtc tgcgatgagc cggtgtccgc
      961 gctggacgtg tcgatccagg cccagatttt gaacctgctg atggacctgc aggacgaaaa
     1021 ggggctctcg tacatgttca tcacccatga cctgagcgtg gtcaaacaca tctccagcca
     1081 catcgcggtc atgtacctgg gccagtgtgt ggagtatgcc gagacgcagg agctgttcgc
     1141 ccacccggct catccctata cccgggcact gctggcggcg atcccggtga tcgacatcac
     1201 ccggcgcggc aagcagctgg agaccattca gggcgaggtc acctcgccca tcgaccccgc
     1261 cccggggtgc cgttttgccc cccgctgcaa atacgcctgc gagggctgca ccgccgggca
     1321 gatggagctg cgggagctga cggccgggca ctttgtggcc tgcccctatg cgagatgaac
     1381 ccccctgcgg ctgaaaggag cgatgacatg tcccgtatcc gtcaaaatta tgcaaggctg
     1441 gtgctgaccc gcggggtcaa cctgcagccg ggccagatcc tggtgatcga cgctccggtg
     1501 tggaccagcg agttcgtgca tctggtgagc gaggaggcgt tccggctggg ggccaaggac
     1561 gtggtgatcc actatgccga ccccgacgcc gaccgtctgc ggctgctgca tgcggacgag
     1621 gagaccctct gcgacgtgcc ccgctggcag gtggagagcc agaccctcta cggggagaag
     1681 aacgcctgct ttttgcggct ggactcaaac gaccccgacg gcatggccgg tgtggacccc
     1741 gcccgcctct ccctctggaa aaaggcggtc agcgccccgc tggaggggct gcggctgaaa
     1801 aggatgtcca acgacctggc ctggagcggc gtgcccgcgc ccaacgcccg ctgggcgaaa
     1861 aaggtcttcc ccgcgatggg ggagcccgag gcgctggagg ccctgtggca ggcggtctac
     1921 cgctgctgct atgtgagcga ggagagcgcc gaggcggggt gggacgccca tgtggaggag
     1981 atgcagcgca atgtgcgcaa gatcaacgcc ctgggggtgc gcagcctgcg ctttcaaaac
     2041 ggcaagggca ccgacctgac gctggagctc tgcgacggcg gcgtgtttgc gggcggcatc
     2101 tgccactgcc ccgagccgga cggcatcgtc ttcgcgccca atatccccac cgaggagatc
     2161 ctcaccacgc cgcaccggga caaggtgaac ggcgtcgttt acaacacctt gcccctggtc
     2221 tacggcggca gcatcatcga cgacttctgc ctcacgttcc gagacggcgt ggtggtggac
     2281 tggcgctgcg gccagggggc cgaggtgctg gcgggcattc tggacaccga cgcgggcacc
     2341 cggcgcctgg gcgaggtggc gctggtgccc tttgactcgc ccatcaacca gctgggggtg
     2401 ctgttctatg acaccttgtt cgacgagaac gcctcctgcc atctggcgct gggcgcgggc
     2461 tatgtggacg tgatccccgg cggcgaccgc agcaaggagg cgcttgtcgc ccgcggcctg
     2521 aacacttcga tgctgcatgt ggatttcatg ttcggcagcc gcgacatgcg ctgcacggcg
     2581 cgggacgccg acggccgcga ggtggagatc ttccgcgacg ggctgttcgt aatctgaaaa
     2641 aaacggtatc catgagaccg ggagggcccg gggcggcaaa aaccgccccg ggctttctgt
     2701 gcggataaag accccaaaag ccgggcgcgc agccttgaaa cgggcggaaa attccattat
     2761 acttagacgg gacaaagcgc cccggcacag ggggacggag gggcgctcgg gccggccggt
     2821 cgggagccgg gctgcggaaa atgacgggcc gtgaatgggg tgtggggatg gagaagagag
     2881 tgagcggcaa aacagggaca gggctgagct gtctggcgct gctgccggcg gtcgcgctgg
     2941 tatggggcgt ggctgtcgcg ggcggctgcg cctggtgggc cttgcccgcg gcgctgtgcg
     3001 gcgccgggct ctgcatcctt ttgccgggct gcgccctgct atacgctctc aagcgtttga
     3061 ctcctctgcc gcaggggttt gaactgccgc tggcgctgct gctgggcagc ggcttttttg
     3121 ccgcgctcac ctgcctctgc gcccgctttg gcctggcatg gccgctgcgg gtcgtgccgc
     3181 ccgccgtcgc gctggccggc tttttcgctt tccgcaggga cgcggggcaa acggcggaga
     3241 ggctgaggca ctggcgcctg caccgggcgg acgcgctgct gctggtggcg ctgctggtgg
     3301 tctatgccct ctgctgggag accgccaccg cccatccggc agcggtgggg cagacggtgc
     3361 tcaaacagga ttttttgtgg aatatcggca acgcgcgcag cttctggctg ggctttccgc
     3421 cgcaggacat ccgctttgcg ggggtgcggc tgcactatca ttatctgaac gagctgctgg
     3481 ccgcggggct ctcggccgcg tgcggcgctt ccgtctacga tctgctgggg ttcgtgtggc
     3541 cgcccctggt gctggcggcg gcgctggcgg gcctgcgggg gttgggacgt ctgttctatg
     3601 aggaggacgg ggaagcgcgg gcgcggcgcc tcgcggcgtt tttgcccttt gccctgttcg
     3661 gcgcgggcag tatcgacctg atttggcgcc tgttctcctc ctcgggcttt gagacgagca
     3721 gcctctacca ctacatcacc aatgtgaatg cctgcgggac ggcgctcttg ttcagcagtg
     3781 tttttctcgg attggtcctt tgggcgatgc ggaggcaata ccgggtcaac gcggcgtttt
     3841 ttgtgctgac ggcctgctgc acggtggtcc tctgctttgc caaagggccg gtggccctca
     3901 tcctggcgct ggcctttgcc gctgcgttgc tctggcaggc ggttcagaaa aaggcgcggc
     3961 tgcggggcat tctgctggcg ggggtggtgc tgctgacggc tggaggtatc tattttgcga
     4021 tcttttcctc cggcgcgaac aacatggcgc tcacgccgga cggcacgctg ctcaaaagcc
     4081 cgctcgcccc gctgctgatc gcgctgggac ggcgcagcaa ttttgcctat tatctgtgcc
     4141 tgcccttttt tatggccctg caccagctct gtgtcagccc ggtggtgacg gtgctctatg
     4201 cgggctgcct ctggcgggac gtgcggcggc tgcgcagcct cgacggcccg cgcctgctgc
     4261 tgcacgccgg cgcgctgggc ggcatgctgg cctactatct gttcgaccat tacgccatga
     4321 gccagattta ttttttgtat ctctcgcagc aatgcgccat tttgctgggg atagagctgc
     4381 tcttctccat ccgccgcccg gcgttgaaaa gggcggcttt caggctgggg gtgttctgtg
     4441 cgctctccgc ctctgcggcc gccctggccc tctgcgggga cggcgtgggg ctgttggcta
     4501 cgcgtctcga cgatgagccc tgtggggcgc agtacaatgt gacggcgggg aacgaggcgg
     4561 ccgcccaatg gctgggtgcc gagatgccgc gggaggccgt ctttgccacc aatcgctact
     4621 atgacgtctc gcccgaggcg gggaactcca atctctacac cgcgctctcc ggccgtcagg
     4681 cctatatgga aggctttttg tacgccgtct cgaatatggg ggtgccccgg cagtgggtgg
     4741 aggagcgctt tacccgcaac gccgtcctgt tttcgccgaa atccgacccc gcaaccgtgc
     4801 tccactccgc gcgggaggcg ggtatttcgt atctggtttt tgacgaggcc tacgccttta
     4861 ccggcgaaaa cggcccccag ttcacagcgc tggaaaaggt ctatgaccag ggcggcgtcc
     4921 agatctaccg ggtgccgcag gcctgagaga agacgcaagg cctttttttg cccctgtccc
     4981 agaggtgcgc ggagagaccc ctttaccgag agacgccctt gctccgcttg acaatcccgc
     5041 gcaaagcgga taaaatcaaa ggcgagaaat gcagtgatgg agagggctgc ggcccgcttc
     5101 ccccaagaga gcctgcggtg ggtgcgagca ggcgggaggc agccgcggcg gtcgctccgg
     5161 agcaggcggg tgaacgcttt ttggccatta gccgcgcacg ttttccgcgt taaggagctt
     5221 tgagcggccg cgtttttttg gcgcggcaat gtgggtggta ccgcggagct tttccgtccc
     5281 atacggcgct tgccgtatgg ggctttttat ttgtccccgg gggcgggcgc acatcgagca
     5341 aaagagaggg agaacactga tgagagagct ggacaagcaa tacgaccctt cccaggtgga
     5401 ggacagcacc tatcgcttct ggctggagca caaatatttc cacgctgagc gcgacccgga
     5461 aaagaagccc tataccatcg ttatcccgcc ccccaacatc accggccagc tgcacatggg
     5521 ccacgccatg gacgagacgc tgcaggatat cctcatccgc accaaacgga tgc
//
LOCUS       DBMDYA010000092         5565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3632_length_5565_cov_1.691984, whole genome shotgun sequence.
ACCESSION   DBMDYA010000092 DBMDYA010000000
VERSION     DBMDYA010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_5565_cov_1.691984"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="ACZPFK_03405"
     CDS             complement(<1..577)
                     /locus_tag="ACZPFK_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405392.1"
                     /translation="MPKAKTIGTDLTQGNILRLLLIFVLPIFFTNLIQQFYNIVDVIV
                     IGQFAGSEGTVGVSTGGEVINLLTFVSMGFASAAQVYISQLYGVRDHEKIRETIGTTL
                     SLMGLCSLVFMALSMLFCRPLLTLMNTPAEALGQSYDYMMVTALGMPFIFGYNAVCGI
                     LRGMGESKRPLEFIAISATANIVLDLFFVAVL"
     gene            complement(657..2039)
                     /locus_tag="ACZPFK_03410"
     CDS             complement(657..2039)
                     /locus_tag="ACZPFK_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405393.1"
                     /translation="MESTIKKRGVGTNLTEGPIFKTLLLFAIPIILTNLIQQLYGMVD
                     LMVIGQFVGSAGTVGVSTGGEMADLVTPVAMGFSTAGQIFIAQLAGARDDRRIKDTVG
                     TLLTFMLGLSFVLVLVGVFFCKPILQLLNCPAEALGQAEAYMVITVLGFPFIFGYNAI
                     CGVLRGMGESKRPLLFIIVAAVVNIVFDLVLVVGFDLQAAGTAVATTLSQLGSFLAAL
                     IYLYKRREKFDFVPRLSYFKVRRDILWVLIKLGIPQVVRSMLVRFSLLWINANVNAYG
                     LVVSATNSVGNKIQKFAEVFIQGVDTASAAMIGQNLGAKKPERAKKVTWATLALTLSC
                     AVAVSLLCVAAPERIFGIFTADEAVRALGAVYLHIMIIHFFSSAFVGAFQAMVTGCGF
                     VSLGFAIGILDGVVCKIGLSLIFVNLMGMGYVGYFLGIGCSRILPGLLCFAYFLSGRW
                     RTRKLLSERG"
     gene            complement(2126..3001)
                     /locus_tag="ACZPFK_03415"
     CDS             complement(2126..3001)
                     /locus_tag="ACZPFK_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1405394.1"
                     /translation="MTTITNGALTVACDSLGGELQSIRDASTGLEYLWQGDRTYWGRR
                     ALNLFPLVGRLYEKTYTLHGRPYEMPLHGFLPHARMELDALAPDRCRFVLRDSAETRR
                     VYPYSFVFTLDYRLSGRALEIGFAVENRSDETLYCAMGGHPGFNLPLEEGLRFEDYEI
                     RFPTPCSPQLVQFSPSVLDAGGRAPYPLVEGRRLPLRHSLFTQDAVVLAGAPRCVELS
                     SPRGCHGVRVEYPQMPYVGFWHKPNTEAPFLCIEPWSALPGREGVVEELSRMADLTAV
                     PAGARAANRWSIAVW"
     gene            complement(3024..4037)
                     /locus_tag="ACZPFK_03420"
     CDS             complement(3024..4037)
                     /locus_tag="ACZPFK_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405395.1"
                     /translation="MEGRMWPIRTGNKARRIKWGPPQNQISWAYMKQLRERDATQRIY
                     ACDPYVEVYRFGDNFYGLFANNCDGMGDVWMYLIVGPERAMLIDTAFGLGDTKALVDE
                     ITGGLPLIVADTHSGPDHCLGNVRFERVYCHEYCEWGIRNKCKPGAWDYLFDENGDNI
                     WLDFDRKDLPEYRDYELAPVKNHHRFDLGGGYEVELVWMPGHDSGHAMFLDHGGRRLI
                     AGDDVCSDVIGCGSGGKAGDPYARYCNLESYRDELAKLCLRLDEFDYIFPGHFMVNLE
                     NSLMCDILATLDRILDDPEACDYRLEEASGNGGPKKVRMFKFIPGFSVIAYTANGVRR
                     PKE"
     gene            complement(4177..5145)
                     /locus_tag="ACZPFK_03425"
     CDS             complement(4177..5145)
                     /locus_tag="ACZPFK_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405396.1"
                     /translation="MAALLEVKHLKKYFPTNKGVVHAVDDVTFSLDAGKTMGVVGESG
                     CGKSTLGRTIIHLQDSTDGQILFEGEDVTHVKGKELKKLRENMQIIFQDPYSSLNPRM
                     SVHDTIVEPLILSGKFKGPALEKEASRLMDMVGIESRLRGAYPHEMDGGRRQRVGIGR
                     ALALNPKFIVCDEPVSALDVSIQAQVLNLLMDLQDEFKLTYMFVTHDLSVVRHISDSI
                     CVMYLGQLVETCDTKELFAHQLHPYSKALLSAIPSTDIHHPTKRIVIKGEIVSPIDPK
                     PGCRFAARCPHACERCHQPQQLEEVTPGHYVACCRVRELNEELSRA"
     gene            complement(5148..>5565)
                     /locus_tag="ACZPFK_03430"
     CDS             complement(5148..>5565)
                     /locus_tag="ACZPFK_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1405397.1"
                     /translation="AQVLDMINKLKEELNTSMIMITHDLGVVAETCDTVAVTYAGQVI
                     EYGSKEDIFDRPAHPYTVGLFKALPNMNKDAARLAPIDGLPPDPTDLPEGCSFSPRCP
                     YATEECRRTPVESRELTPGHLCRCIKAAAEAATQEV"
BASE COUNT         1006 a         1751 c         1853 g          955 t
ORIGIN      
        1 ccagcaccgc cacaaagaac aggtccaaaa cgatgttggc ggtggccgag atggcgatga
       61 actccagcgg gcgcttcgac tcgcccatgc cccgcaggat gccgcagacg gcgttgtagc
      121 cgaagatgaa aggcatgccc agggcggtga ccatcatgta gtcgtacgac tggcccagcg
      181 cctcggcggg ggtgttcatc agggtcagca gcgggcggca gaacagcatg gaaagagcca
      241 tgaacaccag cgagcagagg cccatcaggc tgagggtggt gccgatggtc tcgcggatct
      301 tctcgtggtc gcgcaccccg taaagctggg agatatagac ctgcgcggcg ctggcgaagc
      361 ccatgctcac aaaggtgagc aggttgatga cctcgccgcc ggtggagacg cccaccgtgc
      421 cctcgctgcc cgcgaactgg ccgatcacga tcacatcgac gatgttgtag aactgttgga
      481 tcaggttggt aaaaaagatc ggcagcacaa agatcagcag caggcgcaaa atgttgccct
      541 gggtgagatc ggtgccgatg gttttagctt ttggcatggc ataccccttt ctgccggcgt
      601 ctcatccggc ttgcaggccg ctgcggcccg gcccggcccc gcggggagcg gcgcggtcac
      661 ccgcgctcgg acagcagctt gcgggtgcgc catctgccgc tgagaaaata ggcgaagcag
      721 agcagccccg gcaggatgcg cgagcagccg atgccgagaa aatagcccac atagcccatg
      781 cccatcaggt tgacgaagat caggctcagg ccgatcttgc acaccacgcc gtccaggatg
      841 ccgatggcaa agcccagtga gacgaagccg cagccggtga ccatggcctg gaacgcgccc
      901 acaaaggcgg aggagaaaaa gtggatgatc atgatgtgca ggtagaccgc ccccagggcg
      961 cgcaccgcct cgtcggcggt aaagatgccg aagatgcgct cgggcgccgc cacgcagagc
     1021 agcgagaccg ccacggcgca agacagggtc agcgccagcg tggcccaggt gacctttttg
     1081 gcccgctccg gctttttggc gcccaggttt tggccgatca tggctgccga ggccgtgtcc
     1141 accccctgga tgaagacctc ggcaaatttt tggatcttgt tgcccacgct gttggtcgcc
     1201 gagaccacca gcccgtaggc gttgacgttg gcgttgatcc acagcagcga gaaccgcacc
     1261 agcatcgagc gcaccacctg cgggatgccc agcttgatga gcacccaaag gatgtcgcgg
     1321 cgcaccttga agtaggagag ccggggcacg aagtcgaatt tttcgcggcg cttgtacaga
     1381 tagatcagcg cggccaggaa agagcccagc tgggacaggg tggtggcgac ggcggtgcct
     1441 gcggcctgca ggtcgaagcc caccaccagc accaggtcga agacgatgtt gaccaccgcg
     1501 gccacgatga tgaacagcag cgggcgcttg gactcgccca tgccgcgcag cacgccgcag
     1561 atggcgttgt agccgaagat aaaggggaag cccagcaccg tgatgaccat gtaggcctcc
     1621 gcctggccca gggcctcggc ggggcagttg agcagctgca gaatgggctt gcagaagaag
     1681 acgcccacca gcaccagcac aaaggagagc cccagcataa aggtcagcag ggtgccgacg
     1741 gtgtccttga tgcgccggtc gtcgcgggcc cccgccagct gggcgataaa gatctgcccg
     1801 gcggtggaaa agcccatcgc caccggggtg accaggtcgg ccatctcgcc gccggtggat
     1861 acccccaccg tgccggcgct gcccacaaat tggccgatga ccatcaggtc caccatgccg
     1921 tacagctgct ggatcaggtt ggtgaggatg atggggatgg cgaagagcag cagcgtcttg
     1981 aagatcggcc cctcggtgag gttggtgccg accccccgct ttttgatcgt agattccatg
     2041 ggtattctct tttctctcta ggatagtgtt ccggtccggc cgcagggccc ccttgccggg
     2101 gcgccctgcc ggcggcgccg gggccctacc agaccgcgat ggaccagcgg ttggcggcgc
     2161 gcgccccggc gggcacggcg gtcagatccg ccatgcggga gagctcctcc accacgccct
     2221 cccgccccgg cagggcggac cagggctcga tgcacagaaa gggcgcctcg gtgttgggct
     2281 tgtgccaaaa gcccacatag ggcatctgcg ggtactccac ccgcaccccg tggcagcccc
     2341 gcggcgagct gagctccacg cagcggggcg cccccgccag caccaccgcg tcctgggtga
     2401 acaggctgtg ccgcagcggc agccggcgcc cctccaccag cgggtagggc gcgcgtcccc
     2461 cggcgtccag caccgacggg ctgaactgca ccagctgcgg gctgcacggg gtggggaagc
     2521 ggatctcgta gtcctcaaag cgaagcccct cctccagcgg caggttaaag ccggggtgcc
     2581 cgcccatggc gcagtagagc gtctcatccg agcggttctc caccgcgaac ccgatctcga
     2641 gcgcgcggcc cgagagccgg taatccaggg tgaacacaaa gctgtaggga tagacccgcc
     2701 gggtctcggc gctgtcccgc agcacaaagc ggcagcggtc gggggcaagg gcgtccagct
     2761 ccatgcgggc gtggggcaaa aagccgtgaa gcggcatctc atagggcctg ccgtgcaggg
     2821 tataggtctt ttcgtagagc cgccccacca gcggaaagag gttcagcgcc cgccgccccc
     2881 aataggtccg gtccccctgc cagaggtatt ccaggccggt ggacgcgtcg cggatggact
     2941 gcagctcgcc ccccaggctg tcgcaggcaa cggtgagcgc tccgtttgtg atcgtcgtca
     3001 tctgtgcggc gcctcgcttt ccgtcattct ttggggcgtc ggacgccgtt tgccgtgtag
     3061 gcgatgacgc tgaagcccgg gatgaatttg aacatgcgca cttttttggg gccgccgttg
     3121 ccgctggcct cctccaaccg gtaatcgcag gcctccgggt cgtccaggat gcggtccagc
     3181 gtggccagga tatcgcacat caggctgttc tccagattga ccatgaagtg gccggggaag
     3241 atatagtcga attcgtccag ccgcaggcag agcttcgcca gctcgtcccg gtaagactcc
     3301 aggttgcaat agcgggcgta ggggtcgccc gccttgccgc cgctgccgca gccgatcacg
     3361 tcgctgcaca cgtcgtcccc cgcgatcagc cgccggccgc cgtggtccag aaacatggcg
     3421 tgccccgagt catgccccgg catccagacc agctccacct cgtagccgcc gcccaggtcg
     3481 aagcggtggt ggttcttcac cggcgccagc tcgtagtccc ggtattccgg caggtccttg
     3541 cggtcaaagt ccaaccagat gttgtcgccg ttttcgtcga acagatagtc ccaggcgccg
     3601 ggcttgcact tgttgcggat gccccactcg cagtactcgt ggcagtacac ccgctcaaag
     3661 cgcacgttgc ccaggcagtg atccgggccg gagtgggtgt cggcgacgat cagcggcagg
     3721 ccgccggtga tctcgtccac cagggctttg gtgtcgccca ggccgaaagc cgtgtcgatg
     3781 agcatggccc tttcgggccc gacgatcagg tacatccaca cgtcgcccat gccgtcgcag
     3841 ttgttggcga acaggccgta gaagttgtcg ccgaagcggt agacctccac ataggggtcg
     3901 caggcataga tgcgctgggt ggcgtcccgc tcgcgcagct gcttcatata ggcccagctg
     3961 atctggttct ggggcgggcc ccacttgatc cgccttgcct tgttgccggt gcggatgggc
     4021 cacatgcgtc cttccataca gattacctcc gttcctgaat tgcgttaaaa atggatcccg
     4081 cgggcaaagg tccccggatg aggaacatcc ggggcaaaaa aaggatgaga tatgccctca
     4141 aaaaacatgc tctcaggaca tatctcaccg ctcaggtcag gcgcggctga gctcctcgtt
     4201 gagctcgcgc acgcggcagc aggccacata gtgcccgggg gtgacctcct ccagctgctg
     4261 cggctgatgg cagcgctcgc aggcgtgcgg gcagcgcgcc gcaaagcggc agccgggctt
     4321 ggggtcgatg ggggagacga tctcgccctt gatgacgatg cgcttggtgg ggtggtggat
     4381 atcggtggag gggatggccg acagcagcgc cttggaatag gggtgcagct gatgcgcaaa
     4441 cagctccttg gtatcgcagg tctccaccag ctggcccaga tacatcacgc agatgctgtc
     4501 cgagatgtgc cgcaccaccg acaggtcgtg ggtgacgaac atataggtca gcttgaactc
     4561 gtcctgcaga tccatcagca ggttgagcac ctgtgcctgg atcgagacgt ccagcgccga
     4621 gaccggctcg tcgcagacga tgaacttggg gttgagggcc agggcgcggc cgatgcccac
     4681 gcgctggcgg cggccgccgt ccatctcgtg ggggtaagcc ccgcgcaggc gggactcgat
     4741 gcccaccatg tccatcaggc gggaggcctc tttttccagc gccgggccct tgaatttgcc
     4801 ggacaggatc agcggctcga cgatggtgtc gtgcaccgac atgcgcgggt tcagcgagga
     4861 gtaggggtcc tgaaagatga tctgcatgtt ctcgcgcagc tttttcagct ctttgccctt
     4921 gacgtgggtg acgtcctcgc cctcaaacag gatctgcccg tcggtggagt cctgcaggtg
     4981 gatgatggtg cggcccaggg tggatttgcc gcagccggat tcgcccacca cgcccatcgt
     5041 cttgcccgcg tccaggctga aagtgacgtc gtccaccgcg tgcacgacgc ccttgttggt
     5101 ggggaaatat tttttcaggt gcttgacctc tagtaacgca gccattttca tacctcctgg
     5161 gtggccgctt cggcggccgc tttgatgcaa cggcaaaggt gccccggggt gagctcccgg
     5221 ctctcgacgg gggtgcggcg gcattcttcg gtcgcatagg ggcagcgcgg ggaaaagctg
     5281 cagccctccg gcaggtcggt ggggtcgggc ggcaggccgt cgatgggcgc cagccgggcg
     5341 gcgtccttgt tcatgttggg cagcgccttg aacaggccca cggtgtaggg gtgggcgggc
     5401 cggtcaaaga tgtcctcctt gctgccgtac tcgatgacct ggccggcata ggtgacggcc
     5461 acggtgtcgc aggtctcggc caccacgccc aggtcgtggg tgatcatgat catcgaggtg
     5521 ttcagctcct ccttgagctt gttgatcatg tccagcacct gggcc
//
LOCUS       DBMDYA010000093         5543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3643_length_5543_cov_1.799522, whole genome shotgun sequence.
ACCESSION   DBMDYA010000093 DBMDYA010000000
VERSION     DBMDYA010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_5543_cov_1.799522"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="ACZPFK_03435"
     CDS             complement(<1..451)
                     /locus_tag="ACZPFK_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405398.1"
                     /translation="MSALLESKDVCKYFGGLKAVEKVNMSVNKGDVFGIIGPNGAGKT
                     TFFNICSGVYGPTSGAVYLDGEDISGKYPEEVAKRGMARTFQNIQLFKFMTVLDNVKV
                     GFHLSTKTTMLDAMLHTRRYRADEEFIRERGAQVLERVGLSEYRDTPA"
     gene            complement(448..1434)
                     /locus_tag="ACZPFK_03440"
     CDS             complement(448..1434)
                     /locus_tag="ACZPFK_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1405399.1"
                     /translation="MKQCKALIQKNKKPLLAALLAAAFLLPLFSGSYVLGILCRILLY
                     GTLAGSLNVVNGYSGQFNIGHAGFVCIGAYAGALCGTRLGWPFVVQLLAGGALSAVMA
                     FLLSKPILRLKGIYLAIITMGFCEITRIVTLNWTSVTGGPLGIKNIPAPEFFGLKFNS
                     PKLYYYIFLLLLIVFTFLTNRLLKSRVGRAWMSIREDEVAAKSLGVKIGHYRSVNLMY
                     GAMWAGVCGAAMGPYYRYIASDMFSMDMSFNILSMVILGGQGTLVGPIVGASIINTVT
                     EVFRFASEYRMVLYALIIIVMMWLRPQGLIGVSDSMLSGRTITKKKAKGVKT"
     gene            complement(1431..2306)
                     /locus_tag="ACZPFK_03445"
     CDS             complement(1431..2306)
                     /locus_tag="ACZPFK_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1405400.1"
                     /translation="MEYILIQLVNGLCQGSIYALMAIGYASIVGVVGLVTFAYGEVMM
                     VGAFAAYYTFLYLADNILLGVVASFAASAVIGFLVHKVCYEKFFDKPRHIPLICTIGF
                     SMLVKNLAQIFFGANTKATPQVVKNFFIDLGPVRINAVQVTIVATVIALSITLYLVFN
                     KTKLGIRLRAVSQNREAAALVGINVKRTALLGNCLGCGLGGIAGLLLAVYYQSLSPTM
                     GSTFGNKAFASSVVGGMTSIPWAAGGGVMIGVIENIGTSFLPNSYRDVFAFVFLFIVL
                     LIRPEGLSKKKGIRP"
     gene            complement(2393..3583)
                     /locus_tag="ACZPFK_03450"
     CDS             complement(2393..3583)
                     /locus_tag="ACZPFK_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1405401.1"
                     /translation="MKKALSVLLALALGLTILTGCQSGGASSGGGASTSGAAQGGETV
                     KFAVLAPITGNYAEFGTAFKVATKMAADEINAAGGVNGRQIVLDVYDTKGDAAESSDI
                     ARQLEEQEDVVGVIGDFSSTACMANAPIFGDAGLVQLSPTASHSDFTPMNDYMFSIMG
                     RTDDESRFFISSLVKEYKQKDNIGIVYVNTDWGNQCNSSMMDQADIDGLTVVADEPYT
                     EGEMDFTNVLNKVKNQNPDLLVLIMQTVDCANALNTLAQMGWEIETVCQGASASTQVI
                     ELAGENAEGLCSTTSFFIDETVPEEKAWLDQFVAEAGFSPTVHAAVAYDSVYVMAEAA
                     KSCGDDITRQGIRDGLAGLGEYMGFTGPIEFDETGAIFRKVLIVQIEDGKYVRKTDYT
                     YGNQ"
     gene            complement(4222..5064)
                     /locus_tag="ACZPFK_03455"
     CDS             complement(4222..5064)
                     /locus_tag="ACZPFK_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405402.1"
                     /translation="MAYKRQTKEEKERMRRRRRRQLLGGALCVLIVLGAVSLVSAGVR
                     VTAKLFDDTKEKEQYEKLLAPIVMLDPVPFDSLETADQNMLLQTAIWETIYNEDITKY
                     ERDDVGALILPAVDIDKNGALLFGKDYVLEHHTFDANGMQFVYDEEKSGYIIPITGTT
                     SAYTPKVVKIKNSVQQKRVTVGYVAPPTGFSLDGTLSTEQGEPAFYYDYIFTRQGDGY
                     YLAAMTTSEMKPQTAAGAGATEAPPVVPQDEELANEIQQQADSLTGGSSAPQSEPAAD
                     GDAQ"
     gene            complement(5092..>5543)
                     /locus_tag="ACZPFK_03460"
     CDS             complement(5092..>5543)
                     /locus_tag="ACZPFK_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1405403.1"
                     /translation="TPASSAFLPLAAQKLEEAGVELRLDAAAGKIISGKPANEHDFDT
                     EFLDYILGVKIVRDLDEALAHIAAHSTHHSDAIVTADEDAAARFCREVDSAAVYVNAS
                     TRFTDGGEFGLGCEMGISTQKVGARGPMGLEEITTYKYVVRGNGQIR"
BASE COUNT         1040 a         1717 c         1651 g         1135 t
ORIGIN      
        1 ccgccggcgt gtcccggtat tcagacaggc cgacccgctc cagcacctgg gcgccccgct
       61 cacggatgaa ctcctcgtcc gccctgtaac gccgggtgtg cagcatggcg tccagcatgg
      121 tggttttggt ggacaggtga aaacccactt tcacattgtc gagcacagtc atgaacttga
      181 acagctggat gttctgaaag gtgcgcgcca tgccgcgctt tgccacctcc tcggggtact
      241 tgcccgagat gtcctcgccg tccagataga ccgcccccga ggtgggcccg taaacgccgg
      301 agcagatgtt gaaaaaggtg gttttgcccg ccccgttggg gccgatgata ccgaacacat
      361 cccccttgtt gacgctcata ttgacctttt cgaccgcctt gaggccgccg aagtatttgc
      421 agacgtcttt tgactctaat aacgcgctca tgtctttact cccttggcct ttttcttggt
      481 gatggtgcgg ccggacagca tgctgtcgga aacgccaatc agcccctgcg ggcgcagcca
      541 catcatgacg atgatgatca gggcgtacag caccatgcgg tactcggacg caaagcggaa
      601 cacctcggtg accgtgttga tgatggaggc gccgacgatg gggcccacca gcgtgccctg
      661 gccgcccagg atcaccatcg agaggatgtt gaagctcatg tccatcgaga acatgtcgga
      721 ggcgatatag cggtaatacg gccccatcgc cgcgccgcag acgcccgccc acatggcgcc
      781 gtacatcagg ttgaccgagc ggtagtgccc gatcttgacc cccagcgact tggccgccac
      841 ctcgtcctcc cggatcgaca tccaggcgcg tcccacgcgg gatttgagca gccggttggt
      901 gagaaaggtg aagacgatca gcagcagcaa aaagatataa tagtacagct tggggctgtt
      961 gaacttgagg ccgaaaaact cgggcgcggg gatgttcttg atgcccagcg ggccgccggt
     1021 gacgctggtc cagttgaggg tgacgatgcg ggtgatctcg caaaagccca tggtgatgat
     1081 ggcgagatag atgcccttga ggcgcaggat gggcttggag agcaaaaacg ccatcaccgc
     1141 cgagagcgcg ccccccgcca gcagctgtac cacaaagggc cagcccagcc gggtgccgca
     1201 caaagcgccc gcgtaggccc cgatgcagac gaagcccgcg tgcccgatgt tgaactgccc
     1261 gctgtagccg ttgaccacgt tcagcgagcc cgccagggtg ccgtacagca ggatgcggca
     1321 gaggatgccc agcacatagc tgccggagaa aagcggcaga agaaaagcgg ccgcaagcaa
     1381 cgccgcaagc agcggctttt tgtttttttg gatcaacgcc ttgcactgtt tcatggccgt
     1441 atccctttct ttttggaaag cccctcaggg cggatcagca gcacgatgaa gaggaacacg
     1501 aaagcgaaca catcgcggta gctgttgggc aaaaacgagg tgccgatgtt ttcgatcacg
     1561 ccgatcatca cgccgccgcc cgcagcccag gggatgctgg tcatgccgcc caccaccgag
     1621 ctggcaaacg ccttgttgcc gaacgtgctg cccatggtgg gggacagcga ctggtaatac
     1681 accgccagca gcagcccggc aatgccgccc agcccacagc ccaggcagtt gcccagcagc
     1741 gccgtgcgct tgacgttgat gcccaccagg gccgccgcct cgcggttctg gctcaccgcg
     1801 cgcagacgga tgcccagctt ggtcttgttg aagacgagat acagggtgat ggaaagcgcg
     1861 atgacggtgg cgacgatggt gacctgcacc gcgttgatgc gcacgggccc caggtcgatg
     1921 aaaaagtttt tgaccacctg cggggtcgcc ttggtgttgg ccccaaaaaa gatctgggcc
     1981 agatttttga ccagcatact gaaaccgatg gtgcagatca gcgggatatg gcggggcttg
     2041 tcaaagaact tttcatagca gaccttgtgc accagaaagc cgatcaccgc cgaggcggca
     2101 aagcttgcca ccacccccag caggatattg tcggccagat aaaggaacgt gtaataggcg
     2161 gcgaacgcgc ccaccatcat gacctcacca taggcgaaag tcaccaggcc caccaccccc
     2221 acgatggagg cgtagccgat cgccatgagc gcgtagatcg agccctggca caggccgttc
     2281 accaactgga tcaatatgta ttccataata acctctcatg caatcggcag ctccaacaaa
     2341 tagcccggcg tccggccgcc cgcaggcagc gcgggcgccg ggcctctccc gtttactggt
     2401 tgccgtaggt gtagtcggtc ttgcggacat acttgccgtc ttcgatctgg acgatcagca
     2461 ccttgcggaa gatggcgccc gtctcgtcga actcgatggg gccggtgaag cccatatatt
     2521 cgcccaggcc ggccagcccg tcgcggatgc cctggcgggt gatgtcgtcg ccgcagctct
     2581 tcgccgcttc ggccatgacg tagaccgagt catatgccac cgccgcgtgc accgtggggc
     2641 tgaagcccgc ctccgccacg aactgatcca gccaggcctt ttcctccggc acagtctcgt
     2701 caatgaaaaa gctggtggtg gagcacagcc cctccgcatt ctcgcccgcc agctcgatga
     2761 cctgggtaga ggcgctggcg ccctggcaga cggtctcgat ctcccagccc atctgggcca
     2821 gcgtgttcag cgcattggcg cagtccaccg tctgcatgat gagcaccaac aggtcggggt
     2881 tctggttttt gaccttgttg agcacgttgg taaagtccat ttccccctcg gtatagggct
     2941 cgtcggccac cacggtgagg ccgtcgatgt cggcctgatc catcatgctg ctgttgcact
     3001 ggttgcccca gtcggtgttg acgtagacga tgccgatatt gtccttttgc ttatactctt
     3061 tcaccagaga agagatgaaa aagcgggact cgtcgtctgt gcggcccatg atactgaaca
     3121 tgtagtcgtt catgggggtg aaatcggaat gggaagccgt gggggagagc tgcaccagcc
     3181 ccgcatcccc aaagatcggc gcgttggcca tgcaggcggt ggaggagaaa tcgccgatga
     3241 cgccgaccac gtcctcctgc tcctccagct ggcgggcaat gtccgagctc tcggcggcgt
     3301 ctcccttggt gtcatagaca tccagcacga tctggcgccc attcacgccg ccggccgcat
     3361 tgatctcgtc ggccgccatc tttgtcgcca ccttaaaagc ggtgccgaac tccgcatagt
     3421 tgccggtgat gggggccagc accgcaaatt tcaccgtctc gccgccctgc gccgcgccgg
     3481 aagtggaagc gccccctccg ctggacgcgc cgccggactg gcagccggtc aagatcgtca
     3541 gccccagtgc cagggccaac aggacgctca gtgccttttt cataattttc ctccttgtcg
     3601 gtgttgtgaa tggatctatt ttcaggaaag cccgcttccc tttacagtgg gaaagccctc
     3661 cctgacgacg gaaagtggaa aagccttgct taaagcgtat ctttaagcat attcgaaacc
     3721 ttgttattaa tcttgttatt aataatatca gcagtttttc tttccgtcaa tgcgtcggtc
     3781 gctttttctt cctatctctc aaatttgtcc gcactacttt atgcatcttg cccagagagc
     3841 tctttttccc gcaaaacaaa atcggcccgg gggccggagg gacaacgcgg gaggcgcctg
     3901 acgcctattt taagggtttc gccgcaggct tctgtttttg aaccctcatc aacaggaggt
     3961 gttttgccgc tttcctggtt ttgcagcccc tttcccaggc ggccgccgcc cggccgcgcc
     4021 cttttgctct tgtgctcttt cgccttttcg tctccgcctg ggcaagcatt tgcgccgtcc
     4081 cggcgcgtct gtgccgggca caaaaagaga cggacaggaa aacctgtccg tctctttttt
     4141 tatcttctgt tccggcgccg gtgcgccccc acttgcggcg tggggcggcg gcgcgggcgg
     4201 ctcctcacgc cgtcccgaag ctcactgcgc gtccccgtcg gccgccggct cgctctgcgg
     4261 ggcggagctg ccgcccgtca gtgaatcggc ctgctgctgg atctcgttgg ccagctcctc
     4321 gtcctgcggc accacggggg gcgcctcggt ggcgcctgcg cccgccgcgg tctgcggctt
     4381 catctcgctg gtggtcatgg ccgccagata gtagccgtcc ccctggcggg taaagatgta
     4441 atcgtaataa aaggccggtt cgccctgctc ggtggaaagg gtgccgtcca gcgaaaaacc
     4501 ggtggggggc gccacataac ccacggtgac ccgtttttgc tgcaccgagt tcttgatctt
     4561 caccaccttg ggggtatagg cgctggtggt gccggtgatg gggatgatat agccgctctt
     4621 ctcctcatcg tagacgaact gcatgccgtt ggcgtcaaag gtgtggtgct ccagcacata
     4681 atccttgccg aacagcagcg cgccgttttt gtcgatgtcc acggcgggca ggatcagcgc
     4741 gcccacgtcg tcgcgctcat atttggtgat gtcctcattg tagatggtct cccagatcgc
     4801 cgtctgcagc agcatattct ggtccgccgt ctccaggctg tcaaagggca ccgggtccag
     4861 catcacgatg ggggccagca gcttttcata ctgctctttt tctttggtgt cgtcaaagag
     4921 cttggcggtc acccgcaccc cggcggagac cagcgaaacc gcgcccagca cgatgagcac
     4981 acagagcgcg ccgcccagaa gctggcggcg gcggcgacgg cgcatccgct ctttttcttc
     5041 tttggtctgt cttttataag ccatgttgtt acctcgcaag ctccggcccg gtcagcggat
     5101 ctggccgttg ccccggacga cgtatttgta ggtggtgatc tcctccagcc ccatggggcc
     5161 gcgcgccccc accttttggg tggagatgcc catctcgcag cccaggccga actctccccc
     5221 gtcggtaaag cgggtggagg cgttgacata gacggcggcg gagtccacct cgcggcagaa
     5281 gcgggccgcg gcgtcctcgt cggcggtgac gatggcgtcg gagtgatggg tggagtgggc
     5341 ggcgatgtgc gccaaagcct cgtcgaggtc gcgcacgatc ttgaccccta ggatataatc
     5401 taaaaattct gtgtcaaaat cgtgttcatt tgcgggtttt ccagaaatta ttttgcccgc
     5461 cgccgcgtcc aggcgcagct caaccccggc ttcttccagc ttttgggccg ccagaggcaa
     5521 aaaggcggaa gaagccgggg ttg
//
LOCUS       DBMDYA010000094         5485 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3692_length_5485_cov_1.832467, whole genome shotgun sequence.
ACCESSION   DBMDYA010000094 DBMDYA010000000
VERSION     DBMDYA010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3692_length_5485_cov_1.832467"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="ACZPFK_03465"
     CDS             complement(<1..511)
                     /locus_tag="ACZPFK_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase family protein"
                     /protein_id="HIR1405404.1"
                     /translation="MTMHNDANPGEIAERIVLVGDPNRAKHMAETYLQDAVLVNKTRC
                     AYCYTGTYEGVRVSVMAVGMGGPSMLIYATELCRDYGCKILVRAGTSGGYLEEMRNFD
                     IVLSQATSTTSGINDNMFNGHFSPVADFGLLCTADRIAKEMKLTTYVGGTICNDRLYR
                     DETDKSRMWK"
     gene            complement(596..1342)
                     /locus_tag="ACZPFK_03470"
     CDS             complement(596..1342)
                     /locus_tag="ACZPFK_03470"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1405405.1"
                     /translation="MVSLWEALGKAKEYRWVDLTHELENDSPYWSGIPSGSVELGKQV
                     MDYAEMGFEIQTFKFPGQFGTHIDYPAHFVPGARRAAAFGLKETVLPLVVLDVSQKAA
                     SDRDYELTVQDVLEFEERYGRIPKGCFVALRTDWSKGWPSMEKLCGLDGEGNEHFPAW
                     GYEALRFLYEERGIAANGHETLDTDAPVSSSVRGLRCERYVLERDAFQVEAMCNLDQV
                     PPVGAVIVVAAPRIACANGLPVRAFAVCPD"
     gene            1728..2105
                     /locus_tag="ACZPFK_03475"
     CDS             1728..2105
                     /locus_tag="ACZPFK_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1405406.1"
                     /translation="MEIVISSNTSKPIYEQITSQMKAMIMSGELQTGDPIPSMRSLAK
                     SIHVSVITVQKAYEDLQRDGFIETTVGRGSFVSAQNKDFYQEEQQRQAEEHLQIAAEI
                     GRISGISLPKLVELLSLFYNEEE"
     gene            2107..2973
                     /locus_tag="ACZPFK_03480"
     CDS             2107..2973
                     /locus_tag="ACZPFK_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405407.1"
                     /translation="MDYILELNKVSKAYEKSNFFLDQISFALPYGSIMGFVGENGAGK
                     TTTIDCILNTIFKDSGTIKLFGKEMSDSDTELRENIGVVYDGDNLPGYLSAKQIADIM
                     RDLYKNWDDALFQQYLSTFHLNKAQKVKTYSKGMTMKLAMAVALAHRPKLLILDEATS
                     GLDPIMRDEMLDVFLDFVGEADHSILLSSHITSDLEKVADYITFIHDGKVILTAKKDD
                     LLYRYAVIRCKESQFAALDPCDIVAYRKRDYQIDVLISEEKEIQRKYKGIIVDHTTID
                     EIMLLLVKGKRK"
     gene            2973..3629
                     /locus_tag="ACZPFK_03485"
     CDS             2973..3629
                     /locus_tag="ACZPFK_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1405408.1"
                     /translation="MGLLKNNLYAALANIKLFLAFLAVMGGALLITGNGTLLTAFSLI
                     SAPAFALLAISCLRKEAASKWSKYKLTFPISRKEIIKSQFQSHMIGTAFGVLLAAVFM
                     GLTVLIHGNLYFYYGFRDAITLLIGSGVLAFLMGAISYPMFYFWGAERMEAILAIGML
                     GSVGIALGLTWIINFMVGFGQVDDFQYYMSMAFIVAVTLAALALSFALSVMIFRKKEY
                     "
     gene            complement(4193..5080)
                     /locus_tag="ACZPFK_03490"
     CDS             complement(4193..5080)
                     /locus_tag="ACZPFK_03490"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1405409.1"
                     /translation="MLVKKMVSFALALALAGALMTGCWGKDDSSSSSSSKPSSSSSSS
                     SSSSSMADGSSAFGGDSSDSGMIPGEGDSSSMMDGSSSMGASGDSSSSTSASASAKPA
                     AAQVDGDWQTRLVNGSHPLPEDFSVETVSVEHYDDRLFDARAVGHLNDMMKGAADAGL
                     PLYLVSTYRSVKRQTALFNRKINYYIDQGYDAETAKQEAAKWVAAPGTSEHNLGLAAD
                     LVSAKWYSSHDDLTEDFEQTPEFAWLAAHCAEYGFILRYPKGKTGVTGVSYEPWHYRY
                     VGGEAAKYLTENGLCLEEL"
     gene            complement(5123..>5485)
                     /locus_tag="ACZPFK_03495"
     CDS             complement(5123..>5485)
                     /locus_tag="ACZPFK_03495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR1405410.1"
                     /translation="RYARIAREAAKQSGRGRIPEVRAAISFSQLCAEAGGYGAALFCY
                     EGGGEALVPGGSLHSRLAGAASAAIITGAEGGFSPEEAQQAQSAGCTLLGLGPRILRC
                     ETAPLAALSAVMALTGNL"
BASE COUNT         1233 a         1534 c         1492 g         1226 t
ORIGIN      
        1 gcttccacat cctggacttg tcggtctcgt cccggtagag ccggtcgttg cagatggtgc
       61 cgcccacata ggtggtgagc ttcatctcct tggcgatgcg gtcggccgtg cagagcaggc
      121 cgaaatccgc caccggggaa aagtggccgt tgaacatgtt gtcgttgatg ccgctggtgg
      181 tgctggtggc ctgggagagg acgatgtcaa aattgcgcat ctcctccaga tagccgccgg
      241 aggtgcccgc ccgcaccaaa atcttgcagc cgtaatcgcg gcagagctcg gtggcgtaga
      301 tcagcatgga cggcccgccc atgcccacgg ccatcaccga cacccgcacg ccctcatagg
      361 tgcccgtgta gcaataggcg cagcgggtct tgttgaccag caccgcgtcc tgcagatagg
      421 tctccgccat atgcttcgcc cggttggggt cccccaccag cacgatccgc tcggcgatct
      481 cgccggggtt ggcgtcattg tgcatcgtca tggtacatcg ctcctttttt atacttttgc
      541 agttttgaag accttgtctc ccgcgccgcg cctttcggcg tcagggagaa tggcgtcaat
      601 ccgggcagac ggcgaacgcc cgcaccggca ggccgttggc gcaggcgatc cgcggcgcgg
      661 ccaccacgat cacagccccc accggcggca cctggtccag gttgcacatc gcctccacct
      721 gaaaggcgtc ccgctccagc acgtagcgct cgcagcgcag cccccgcacc gacgaggaga
      781 cgggcgcgtc ggtgtccagc gtctcgtgcc cgttggcggc gataccccgc tcttcataga
      841 gaaagcgcag cgcctcatag ccccaggcgg ggaaatgctc gttgccctcc ccatccaggc
      901 cgcagagctt ttccatgctg ggccagcctt tgctccagtc ggtgcgcagc gccacaaagc
      961 agcctttcgg gatgcggccg taccgctctt caaactcgag cacgtcctgg acggtcagct
     1021 catagtcccg gtccgacgcc gccttttgcg agacgtccag caccaccagc ggcagcaccg
     1081 tctctttgag gccgaacgcc gccgccctgc gcgcccccgg cacaaaatgg gccggataat
     1141 cgatatgggt gccgaactgg ccggggaact tgaacgtctg gatctcaaag cccatctcgg
     1201 cgtaatccat cacctgcttg cccagctcca ccgagccaga ggggataccg ctccaataag
     1261 ggctgtcgtt ctccagctca tgggtcaggt caacccagcg gtattccttt gccttgccca
     1321 gtgcttccca aagcgagacc atcttctccg cgcctccttt ttgcgcgggt tcctcgcccg
     1381 cgcttatgct tctggtccca ttataagggg ttttgcggca agttgcaatg tggggcaggg
     1441 gaacagcggc agaaaatgga gaccaaaaaa gccgccttgc gcgccgagga tccggcgagg
     1501 ggcgtgtatc caccaccgcc ggtttaccga ggcaggaact gcagagaaac atgttatcag
     1561 tctgtgtcgc cgtctgaaag tatagttgaa aggaaaggta agggcaggtc gctctcccct
     1621 ttcctgtgtc ttaccttgca gcttttgaaa cccattgaca actatatttc atttgactat
     1681 aatgtacttg tacgataagt acacatatta acaaacggag ggctttcgtg gaaattgtca
     1741 taagcagcaa cacaagcaag cccatttatg agcagatcac atcacaaatg aaagccatga
     1801 tcatgagcgg cgaattgcag accggcgacc ccataccgtc tatgcgttct ctggcaaaat
     1861 ccattcatgt cagtgtgatt actgttcaaa aggcttacga ggatttacaa agggatggct
     1921 ttattgaaac gactgtgggg cgcggcagtt ttgtgtccgc gcaaaacaaa gacttctatc
     1981 aggaagaaca gcagcgccaa gcagaggagc acctgcaaat agcggctgaa attggacgta
     2041 tcagcggtat ttcgctgccc aaattggtcg aattactatc cttgttctat aacgaggagg
     2101 aataacatgg attacatttt agaattaaat aaggtgtcaa aagcatatga gaaatcaaac
     2161 tttttcttag accagatttc ttttgcattg ccctatggct ctattatggg tttcgttggg
     2221 gaaaacggag ctggaaaaac cacaactatt gactgcatac taaacactat tttcaaagac
     2281 agcggcacaa taaaattgtt tggaaaagaa atgtcggatt ccgatacgga gcttcgtgaa
     2341 aatatcggcg ttgtatatga tggcgacaat ttgccgggtt atctgtcagc aaagcaaata
     2401 gctgatatta tgcgagattt gtacaaaaac tgggatgatg ctttgtttca acaatatctc
     2461 agcactttcc atttaaataa ggcgcaaaaa gtaaaaacct actccaaagg aatgacaatg
     2521 aagctggcta tggccgtggc tctggcccac cgcccgaagc tgttgatatt ggatgaagcg
     2581 acaagcggat tagaccccat tatgcgcgac gaaatgctgg atgtctttct tgattttgtc
     2641 ggagaggccg atcattctat tctgctttcc tcccatatca caagcgactt ggagaaggtc
     2701 gcggactaca tcacctttat ccatgatgga aaagtcattc taaccgctaa aaaagatgat
     2761 ttgctgtatc gatatgccgt catacgctgt aaagagagcc aatttgccgc gttggatccc
     2821 tgcgatattg tggcgtatcg gaaacgcgat tatcaaattg acgttttaat ctctgaggaa
     2881 aaagagatac agcggaaata caagggcatc atcgtagacc atacgacgat cgacgagatt
     2941 atgcttcttt tggtgaaagg gaaacggaaa taatgggact tttgaagaac aacttatatg
     3001 ccgcactcgc aaatattaag ctctttcttg catttctcgc cgttatggga ggagcgcttt
     3061 taatcacggg aaacggcact ctgctgacgg cgttctctct gatttccgcg ccagcgtttg
     3121 ccctgctcgc catttcatgc ctgcgaaaag aagcggcttc caaatggagc aaatataagc
     3181 tgacctttcc cataagcagg aaagaaatca tcaaaagcca atttcaaagt cacatgatcg
     3241 ggacggcgtt tggggtgcta ttagcggcgg ttttcatggg actgacggtt ctcatccacg
     3301 gaaatttgta cttttactat ggctttcggg atgcaattac gcttttgatt ggcagtggtg
     3361 tgctggcatt tcttatgggg gcaatttcat atccaatgtt ttacttttgg ggcgctgaac
     3421 gaatggaggc cattcttgca attggtatgc tcggttccgt gggcattgca ctgggcttaa
     3481 cctggattat caattttatg gtggggtttg ggcaagtcga tgattttcaa tattacatga
     3541 gtatggcgtt tattgtggca gttactttgg cagcattggc cctatctttc gccctatcag
     3601 taatgatttt ccgaaaaaaa gagtattgat ttaaaaaatg ctttttagcc gcctggtcac
     3661 aggaaaggaa aagaacatcg ctttagctgc ttaatttgta tacgttttac agggcaaatg
     3721 agaggcaaac ggcggcattg aaattttctg atttcaatgc cgccgtttgt ttttgaaaaa
     3781 agcaggataa ggaaagcgcg ataaagcgcg gctgcccggc gcctttttcc accgcccgcc
     3841 ggaagaaaaa cgcggtcacc cgcctcgacc ctgcgcggtg aaatcaaaaa ggccgcgcct
     3901 ccaggcgcgg ctgcaaaact gttttttatc tcaatggccc cgtttcccgc cgccgcgcga
     3961 caaccgaaac ggacggccga aaaaatagcc gcacagcggc tattttttat tgctccgcaa
     4021 taaaaaacaa cttatctcaa gggccgtgcc ccgaggacgg gccacatggc cgaaaaaata
     4081 gccgcacagc ggctattttt tattgcttcg caataaaaaa caacttatcc caagggccgt
     4141 ggcccaagga tgggccacat ggccaaaaaa atagcctgcg gctatttttt tgttacagct
     4201 cttccaggca gaggccgttt tcggtgagat attttgcagc ctcccccccc acatagcggt
     4261 aatgccaggg ctcataggac accccggtca cccccgtttt gcccttgggg tagcgcagga
     4321 taaagccata ctccgcgcag tgcgcggcca gccatgcaaa ctccggcgtc tgctcaaagt
     4381 cctcggtcag gtcgtcgtgg ctggaatacc actttgccga caccaggtcc gccgccaggc
     4441 ccaggttgtg ctcgctggtg ccgggggcag ccacccactt ggccgcctcc tgcttcgccg
     4501 tctcggcgtc atagccctgg tcgatataat agttgatttt gcggttgaaa agggcggttt
     4561 gccgcttcac gctgcgatag gtcgaaacta agtaaagggg caggcccgcg tcggccgccc
     4621 ctttcatcat gtcgttgaga tgcccgaccg cacgcgcgtc gaacagccgg tcgtcatagt
     4681 gctccaccga cacggtctca accgaaaaat cctccggcag cgggtggctg ccgttcacca
     4741 gccgggtctg ccaatcgccg tccacctgcg cggcggccgg ttttgcgctg gccgacgcgg
     4801 aggtggagga tgacgagtcg ccggaggcgc ccatgctgct ggagccgtcc atcatcgaag
     4861 agctgtcgcc ctcgccgggg atcatgccgc tgtcgctgct gtcaccgccg aacgccgagc
     4921 tgccgtccgc catcgacgag ctggaggaag acgaggacga cgaggagctg ggcttgctgc
     4981 tggaggagct ggacgaatcg tccttgcccc agcaaccggt catcagggcg ccggccaggg
     5041 ccagcgcgag cgcaaaggat accattttct taaccaacaa tccatcattc cttttcccgg
     5101 gcgcggcaga gccgacgccc tgttatagat tgccggtcag cgccatcacc gcgctcagcg
     5161 ccgcaagggg ggccgtctcg caccgcagga tgcggggccc gaggccgaga agagtacagc
     5221 cggcgctctg cgcctgctgc gcctcttcag gggagaagcc gccctccgcg ccggtgatga
     5281 tggctgcgct cgctgcgcct gccagccggc tatgtaagct tccccccggg acaagggcct
     5341 cgcctccgcc ctcgtagcag aacagcgccg ccccatatcc gcccgcctcg gcgcacagct
     5401 gggaaaagga gatggccgcg cgcacctcgg ggatgcgtcc ccgcccgctc tgcttggcgg
     5461 cctctcgggc gatgcgggca tagcg
//
LOCUS       DBMDYA010000095         5470 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3700_length_5470_cov_1.551686, whole genome shotgun sequence.
ACCESSION   DBMDYA010000095 DBMDYA010000000
VERSION     DBMDYA010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3700_length_5470_cov_1.551686"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="ACZPFK_03500"
     CDS             <1..306
                     /locus_tag="ACZPFK_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405411.1"
                     /translation="EISRFLLRSNKKQPHSGCFFGHVARPRATAIEINCFLLRSNKKQ
                     PHSGCFFGHVARPRATAIEISCFLLWSSKKQPQAVFSFPRPFLGPRTAAAAEVMGKQ"
     gene            complement(642..1310)
                     /locus_tag="ACZPFK_03505"
     CDS             complement(642..1310)
                     /locus_tag="ACZPFK_03505"
                     /inference="COORDINATES: protein motif:HMM:NF039407.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HIR1405412.1"
                     /translation="MQFCEHCHVSVAGSHRRCPLCQGPLTGAPDPGEDVFPVLAEDRH
                     RLRLFLRLGAFGTIAAALICLAVNFMFPTGVWWAGFVAAGLTSVWLLVIIALRKRHNI
                     PKTILWLVVLGGLLGLFWDRCTGWHGWGFSYVIPILCSGAMLALMILPRLLRLRLSDY
                     LFYLAIDILFGSLPVVFYLTGLLGDTLAPSLICVCISALSLSAVLVFQGRELVGEISR
                     RMHL"
     gene            complement(1310..2590)
                     /locus_tag="ACZPFK_03510"
     CDS             complement(1310..2590)
                     /locus_tag="ACZPFK_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405413.1"
                     /translation="MKRQKTFQWTRLDNAAKIFPPTSTARDSKVFRFACELNEPVEPA
                     ALQRALDQTLVQFPFYRSSLKKGLFWYYLEGSDQPVQVEAEDEPPCSMLYRGDGRDPL
                     FRVTYYRRRINLEVYHALADGTGALHFLRVLVYHYLALVHAADFPEGPPLIDYDASAA
                     QKFDDSFLKYYSSDKKAPPELNPSAYQLRGSRLPDLRLSIVEGRFSVRALLAKAHEYG
                     TTLTVFLASVLIQAIHSQMSLRDERRPVVVTVPVNLRNYFPSATARNFFSVVNVGYNF
                     SQRSGELEDIIAQVAQGLEAELTADKLARRMNRLADLEHTMLIRVIPLFLKIPVLKFS
                     NWITEREVTVSFSNIGKVEMPPAMQRYIRLVDVFLSTKRLQACMSSFGDNFVITFTTP
                     LASTDVQRNFFRILTGMGLEAAVTTNLAEFEEVD"
     gene            complement(2599..3522)
                     /locus_tag="ACZPFK_03515"
     CDS             complement(2599..3522)
                     /locus_tag="ACZPFK_03515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405414.1"
                     /translation="MAINKAMRAALKALSYPDIDLKKNYKLVRMAENVSHPPANKLFY
                     KAWDHVVPCGDHEVAVRIFAPRDSLPQTKILLFFHGGGWVTGNIDSYDKTCHVMANKT
                     GRTVVSVDYRLAPEYPFPAGLEDCYAVAREIFLDDTLLGTHADEITLIGDSAGGNLAA
                     AVSLLARDRGEFLPRQQILIYPATYNDHTDASPFPSVRSNGSDYLLTAKRVNDYMELY
                     MSRPEDRQDPYFAPLLAEDLTRQPDTLIFTAEYCPLRDEGEAYGRRLLKAGNRARIVR
                     VRDALHGYFMLPPRYVQVKRTYEIINEFLAR"
     gene            3863..>5470
                     /locus_tag="ACZPFK_03520"
     CDS             3863..>5470
                     /locus_tag="ACZPFK_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1405415.1"
                     /translation="MLPRLSVKKPYTILVAVVLVLVLGVVSFTGMTTDLLPNIELPYV
                     VVVTAYPGASPEKVELAVTRPLESVLGTTSGLSHVSSVSQENSSMIILEFEQGTNMDS
                     AMIELSGNVDLVKAQLDDAVGAPMLMKISPDMLPVMVASVDADGMDAKALSRLVDDTV
                     VPAFERLDGVASVSGTGLLEEKLQVTLDQDKIDALNDKVLASVDEKLAEAQGELNSGR
                     AKIADGRAKLETAKSELASKKQSGVDELSAASAKVDGAVASLSALLAEESTLQANQKG
                     FEAEKAGYEAAREQLKPTYDKLNEAVATLSQAASQMSKDIPDNIAGLVALGEEAYNND
                     VLPLVALVGAGMPSELVESLSYANLSALQKGYEDVERRLQEVNAELANIATRTAVISQ
                     MKPQLQQGLDEATKGYQQLESGKMTAVNEITKGEVTITTTEQSLDEADQQLKDAQKQF
                     EEARDAAYEKADLHGILTPELISNILMAQNFSMPAGYLIEGEDQTLVKVGDSYSSEGE
                     LADTLLFHIDAEGVGDIRLSDVAQVELV"
BASE COUNT         1096 a         1624 c         1869 g          881 t
ORIGIN      
        1 gagataagtc gttttttgtt gcggagcaat aaaaaacagc cgcatagcgg ctgttttttt
       61 ggccatgtgg cccgtccccg ggccacggcc attgagataa attgtttttt attgcggagc
      121 aacaaaaaac agccgcatag cggctgtttt tttggccatg tggcccgtcc ccgggccacg
      181 gccatagaaa taagttgttt tttattgtgg agcagcaaaa aacagccgca ggctgttttt
      241 tcattcccgc gacccttttt ggggccgcgg acggccgcag ccgcagaagt aatgggcaag
      301 caataaaatt gcaggggagg gcgcgcgccc tccccgtttg cttgtcgaaa gcccccgcgc
      361 ccggcgcggg tgggggcgga gaaatcaagg ggagccgggg gacagcggaa aggagagaag
      421 acgccccccc ccggagccgg cggcgttccg gctgccgggc gccggcaaaa cagcgaaaaa
      481 aacgggaggt gccggcgcac tcaaaacgcg ctggagcctc tcgtcttttg tcttgtcaat
      541 tggaaaagcg gagccgcgac aggcggcctt ggctttcgct ccccgcggcg cttttcaggc
      601 gccgggcgcc ccgccgccgc ggcacgagag accgctcggc ctcacaaatg catgcggcgg
      661 ctgatttccc ccaccagctc gcggccctga aaaaccagca cggcggagag ggagagcgcg
      721 ctgatgcaca cgcagatcag cgagggggcc agggtgtcgc cgagcaggcc ggtgaggtaa
      781 aagaccaccg gcaggctgcc gaacaggatg tcgatggcga gatagaaaag gtagtccgag
      841 aggcgcagcc gcagcaggcg cggcaggatc atcagcgcca gcatggcccc gctgcacagg
      901 atggggatga catagctgaa cccccagccg tgccagccgg tgcagcggtc ccagaacagg
      961 cccagcagcc cgcccagcac caccagccag aggatggtct tggggatgtt gtggcgcttg
     1021 cgcagcgcga tgatgaccag cagccagaca ctggtcagcc ccgcggccac gaagcccgcc
     1081 caccagaccc cggtggggaa catgaagttg acggccaggc agatgagggc ggcggcgatg
     1141 gtgccgaacg cccccagccg cagaaaaagg cgcagccggt ggcggtcctc cgccagcacg
     1201 ggaaacacgt cctccccggg gtcgggcgcg ccggtgagcg gcccctggca gagcgggcag
     1261 cgccggtggc tgcccgccac cgacacgtga cagtgttcac agaactgcat cagtccacct
     1321 cctcaaattc cgccaggttg gtggtcaccg ccgcttcaag gcccatgccg gtaaggatgc
     1381 ggaaaaagtt gcgctgcacg tcggtggagg ccaggggggt ggtgaaagtg atcacaaagt
     1441 tgtctccgaa agaggacatg caggcctgca ggcgcttggt ggagagaaag acgtccacca
     1501 gccggatgta gcgctgcatc gccgggggca tctccacctt gccgatgttg gagaatgaga
     1561 cggtgacctc gcgctcggtg atccagttcg aaaatttcag caccgggatc ttgagaaaaa
     1621 gcgggatcac ccggatgagc atggtgtgct ccagatcagc cagccggttc atgcggcggg
     1681 ccagcttgtc ggcggtgagc tcggcctcca gcccctgggc cacctgtgcg atgatgtcct
     1741 ccagctcgcc gctgcgctgg gaaaagttgt agcccacgtt gaccacgctg aaaaagttgc
     1801 gcgccgtggc ggagggaaag taattgcgca ggttgaccgg caccgtcacc accaccggcc
     1861 ggcgctcgtc ccgcagcgac atctggctgt ggatggcctg gatgagcacc gaggcgagaa
     1921 agacggtcag cgtggtgccg tactcgtgcg cttttgcaag cagggcccgc accgagaacc
     1981 ggccctccac gatggagagg cgcaggtccg gcagacggct gccgcgcagc tggtaggcgg
     2041 acgggttgag ctcgggcggg gcctttttgt cgctggagta atatttgaga aagctgtcgt
     2101 caaacttttg cgcggccgag gcgtcgtagt cgatgagggg cggcccctcg ggaaaatccg
     2161 ccgcgtgcac cagcgccaga tagtggtaga ccagcacccg cagaaagtgc agcgcgccgg
     2221 tgccgtcggc gagggcgtgg tagacctcca ggttgatgcg gcggcggtaa taggtcaccc
     2281 gaaacagcgg gtcccggccg tcgccgcggt agagcatgct gcagggcggc tcgtcctccg
     2341 cctccacctg cacgggctgg tcgctgccct caagatagta ccagaacagc ccctttttca
     2401 gggaggagcg gtaaaagggg aactgcacca gcgtctgatc cagcgccctt tgcagggcgg
     2461 ccggctccac cggctcgttg agctcgcagg caaagcggaa gacctttgaa tcccgcgccg
     2521 tgctggtggg gggaaagatc ttggccgcgt tgtccagccg ggtccactga aaagttttct
     2581 ggcgtttcaa ctgccgggtc acctcgcaag aaattcgttg atgatctcat aggtgcgctt
     2641 cacctgcacg tagcggggcg gcagcatgaa gtaaccgtgc agggcgtccc gcacccgcac
     2701 gatgcgcgcc cggttgcccg ccttcagcag gcggcggcca taggcctcgc cctcgtcccg
     2761 cagggggcag tactcggcgg tgaagatcaa agtgtccggc tgccgcgtca ggtcctccgc
     2821 cagcagcggg gcaaaatagg ggtcctgccg gtcctcgggc cgggacatgt acagctccat
     2881 ataatcgttg acccgcttgg ctgtgagcag ataatcgctg ccgttgctgc gcacggaggg
     2941 gaacggggag gcgtcggtgt ggtcgttgta ggtggcggga tagatcaaaa tctgctggcg
     3001 cggcagaaac tcgccccggt cccgcgccag cagcgagacg gcggcggcca ggttgccgcc
     3061 ggccgagtcg ccgatcaggg tgatctcgtc ggcgtgggtg cccagcagcg tgtcgtcgag
     3121 aaaaatctcc cgcgccaccg cgtagcagtc ctcaagccca gcggggaacg ggtactccgg
     3181 cgccagccgg tagtccaccg agaccacggt gcgcccggtt ttgttggcca tcacgtggca
     3241 ggtcttgtca tagctgtcga tgttgccggt gacccagccg ccgccgtgga aaaacagcaa
     3301 aatcttggtc tggggcaggc tgtccctggg ggcgaagatg cgcaccgcca cctcgtggtc
     3361 gccgcagggc accacgtggt cccacgcctt gtaaaacaac ttgttggcgg gcgggtgtga
     3421 gacgttttcg gccatccgca ccagcttgta gttctttttc agatcgatgt cggggtagga
     3481 cagcgccttg agcgccgcgc gcatcgcttt gttgatggcc ataaaggcct ccttttcagc
     3541 tgggggaaac gggctcccgg tgggatatcc gattagctta gtataccaca ggacgcggcg
     3601 cttgaaaagc gcggcgcggc cctgcgggcg gccggggccg gcggcgatga tttttttcgt
     3661 tttcagagca gcgcaagggc ggcggaaaaa tgagccgcat ccccttgcca ggggcggcgg
     3721 aatgttttat aatagatgcc gaccgaccgg tcggtcggag attgcggcgg agtgtgctcc
     3781 gccccgccgt gcccccgccc cgctgcaaaa gggggaggaa agggacggac ggcgagaaag
     3841 aagaattcag ggaggaaacc caatgctgcc cagactcagt gtcaaaaaac cgtacaccat
     3901 cctggtggcg gtggtgctcg tgctggtgct cggggtcgtg tcgttcaccg gcatgaccac
     3961 cgacctgctg ccgaacatcg aactgcccta tgtggtggtg gtcaccgcct accccggcgc
     4021 cagccccgaa aaggtcgagc tggcggtgac ccggccgctg gaatcggtgc tgggcaccac
     4081 cagcggcctg tcccatgtca gctcggtctc acaggaaaac tccagcatga tcatcctgga
     4141 gtttgagcag gggaccaata tggactcggc catgatcgag ctgtcgggca acgtggacct
     4201 ggtcaaggcg cagctggacg acgccgtggg cgcgccgatg ctgatgaaga tcagcccgga
     4261 tatgctgccg gtgatggtgg ccagcgtgga cgcggacggc atggacgcca aggcgctgag
     4321 ccgcctggtg gacgacacgg tcgtgcccgc ctttgagcgg ctggacggcg tggcctcggt
     4381 cagcggcacc ggcctgctgg aggaaaagct gcaggtcacg ctggaccagg acaagatcga
     4441 cgcgctgaac gacaaggtac tggccagcgt ggatgaaaag ctggccgagg cccaggggga
     4501 gctcaacagc ggccgcgcca agatcgcgga cgggcgcgcc aagctggaga ccgcaaaatc
     4561 ggagctggcc tcaaaaaagc agagcggcgt cgacgagctg tcggcggcca gcgccaaggt
     4621 ggacggcgcg gtggcgagcc tctcggccct gctggcggag gaatccaccc tgcaggccaa
     4681 ccaaaagggc tttgaggcgg aaaaggcggg ctatgaggcg gcgagggagc agctaaagcc
     4741 aacctatgat aagctcaatg aagcggtggc cacgctgtcc caggcggctt cccaaatgag
     4801 taaagatata ccggacaata ttgccggttt ggtcgctttg ggcgaggaag cttataataa
     4861 cgatgtcctg ccgctggtcg ctctcgtggg tgcggggatg ccctcagagt tagtggaaag
     4921 cctcagctac gcgaacctct ccgccctaca aaagggctat gaggacgtgg aaagaagact
     4981 gcaagaggta aatgccgagc tcgccaacat cgccacccgc acggcggtga tcagccagat
     5041 gaagccgcag ctgcagcagg ggctggacga ggccaccaag ggctaccagc agctcgagag
     5101 cggcaagatg accgccgtca acgagatcac caagggcgag gtgaccatca ccaccaccga
     5161 gcagagcctg gacgaggccg accagcagct caaggacgct cagaagcagt ttgaggaggc
     5221 tcgggacgcg gcctacgaaa aggccgacct gcacggcatc ctcaccccgg agctgatctc
     5281 caatatcctg atggcgcaga acttctccat gcccgcgggc tacctcatcg agggcgagga
     5341 ccagaccctg gtcaaggtgg gggattccta ctccagcgag ggagagctgg cggacacgct
     5401 gctgttccac atcgacgccg agggggtggg ggatatccgc ctgtccgacg tggcgcaggt
     5461 ggagctggtg
//
LOCUS       DBMDYA010000096         5449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3719_length_5449_cov_1.518699, whole genome shotgun sequence.
ACCESSION   DBMDYA010000096 DBMDYA010000000
VERSION     DBMDYA010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3719_length_5449_cov_1.518699"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="ACZPFK_03525"
     CDS             <1..209
                     /locus_tag="ACZPFK_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405416.1"
                     /translation="KAIKAGEMTMTAANSKRAQSATCAQVCQKLAQGERYEGEGKYFY
                     TDSTYQDAMDFNTIDEYLASGNYD"
     gene            284..1819
                     /locus_tag="ACZPFK_03530"
     CDS             284..1819
                     /locus_tag="ACZPFK_03530"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405417.1"
                     /translation="MENDTVLLEMKGITKRFPGVKALDGAQLTVRRGEVHALVGENGA
                     GKSTLMKLLLGIHTPDEGSIVWKGSEVKFHSPHDAISHGISMIHQETSLMPTTSVAEN
                     IWVGREKDFTRFGFLDVRERDRKAREILDELGITIDPRAIIKNQSVAQNQLVEVARAV
                     SYHSDLIIMDEPTSSLAEADIQLLYRIIRGLTAEGVAVIFISHKLEEVLTIADRVTAM
                     RDGKYIATAETGSCTQDDLIKWIVGRDMSTMYPKETAEIGDVVLEARHLGTLDKRVRD
                     VSFQLRRGEILGFAGLVGAGRTETMRALFGIDRLETGEVLIDGKKVRINSPREAIGLG
                     LGMVTEDRLRQGIFSKLSVRQNMSVAYLKDITGRLGLVDFKTEERNCLSMRETLGIKV
                     SALKQMISGLSGGNQQKVIIARWLLSQPRILILDEPTRGIDVGAKAEIHRQISLLARQ
                     GMAIIMISSELQEVLGMSDRILVMHEGEIVGEHLRADADSEQIMKEAFGAPAERMERR
                     NTP"
     gene            1816..2817
                     /locus_tag="ACZPFK_03535"
     CDS             1816..2817
                     /locus_tag="ACZPFK_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405418.1"
                     /translation="MTSEKSLRSDKIKQRAIEVLMNNKAMFILLVLVLIASVLSPNFL
                     LPRNISNILKQVAAATIMGMGYSLVLGSGHMDLSVGYLLGMLGIIAAKLDTQTSLPFG
                     LVVVIVILIGAVGSMANAFFIGVFTLPAFVVTLALGQVFKGINYVVSNTSAISGLCDS
                     FVFLGQGSLGPIPLPVLLSVAVTLIVSLVVYRTAFGRHLLAVGSNLEAARVSGLNVKR
                     IRYGVYCLMGVCVAVAALVLTGRSASAQPQSGQGMEMDAIAAVVIGGTPLGGGHCKPE
                     GTFFGCLIVGLINNILNLMSVNSNWQLVAKGAIILFAIILDSQSEKILERQRLRALK"
     gene            2897..4297
                     /locus_tag="ACZPFK_03540"
     CDS             2897..4297
                     /locus_tag="ACZPFK_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1405419.1"
                     /translation="MLEWRPFDYHSLEELQQDIREAGVNLPISENFNAFKKPLEIYGH
                     TLKNRLSIQPLEGFDAGEDGTPSDLTFRRYRRFAAGGAGLLWFESCSPTATSQTSRRQ
                     CRITDKNLPVFQRLLYEIRDSAADGAPPFTIAQLTHCGRNSRLDGDGTFGDKGLPQYV
                     ACDNPYLPKRHAVILRDEDLDHVMDDYVKAAKLMKKAGFDAVDIRGCHGYLISELLSS
                     FTRENSRYGGPDFENRTRFLLETIDRVQAEVGIPVAIRLNACDMIPYPYGWGMSRDGS
                     LRPDLTEPLRLGKLLADRGVVLLNISMGRNHATQLQLPYNRGSHRPKEHQLYAMAFYH
                     SLAAAFKKEIPQAVVMTGTLSWPGIYAPYIAAGEMEAGMYDLPGFGRLPWAYPDFARD
                     ILENGGLKREKCCIACNSCCNMIGVAASTGCPVGCPVRDPEIYAPLYRQFVGASVRQM
                     SPQTVELFKLEKLGER"
     gene            4479..5135
                     /gene="yunB"
                     /locus_tag="ACZPFK_03545"
     CDS             4479..5135
                     /gene="yunB"
                     /locus_tag="ACZPFK_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HIR1405420.1"
                     /translation="MRYRQKRARQKRRRLPWLLRLAAVFAVVVGLLVALDNEVRPVVI
                     TMAQYQARVSSILAINEAVLDELEKEEVGYDDLVTVTRLEDGTVSSIQADTTAINRLK
                     ARLTNAVSARLTALQLEEVEVPVGTLLGWQIFAGRGPVIRFQIVPASFVESDITSTLE
                     AAGINQTQHKILMQFKVEMSAIIPGYTTSVTVNTQVSIADTLIVGNVPQFYAVGDPLA
                     "
     gene            5233..>5449
                     /locus_tag="ACZPFK_03550"
     CDS             5233..>5449
                     /locus_tag="ACZPFK_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase LigA-related protein"
                     /protein_id="HIR1405421.1"
                     /translation="MEEHQARARIEELRAEIRKNDDLYYNQDAPELEDYEYDRLTQEL
                     KRLEREFPQLVTADSPTQKIDAADSSAF"
BASE COUNT         1183 a         1429 c         1744 g         1093 t
ORIGIN      
        1 aaaaagcgat caaggctggc gagatgacaa tgacggcggc gaactcgaag cgtgcccaga
       61 gcgctacctg cgcacaggtg tgccagaagc tggcacaggg agagcgatat gagggggagg
      121 gcaagtactt ttacaccgac tctacttatc aggatgccat ggacttcaac accatcgacg
      181 agtatctggc cagcggcaat tacgattaaa tcagaaaacc tgcgggcctg cggggagagc
      241 cgcggctctc cccgcaggcc tggtctgtaa gggtggagaa aatatggaaa acgataccgt
      301 actgctggaa atgaaaggga tcacaaagcg gttccccggt gtgaaagctt tggatggcgc
      361 gcagctcacc gtgcggcggg gcgaggtgca tgcgcttgtg ggggaaaatg gcgccggaaa
      421 gtccacgctg atgaagctgc tgctgggcat tcatacgccg gacgagggaa gcattgtctg
      481 gaaagggagc gaggtcaagt tccacagccc gcacgacgcc atctcccatg gcatctccat
      541 gatccatcag gagaccagct tgatgcccac caccagtgta gcggagaaca tctgggtagg
      601 gcgggaaaag gattttacgc gtttcggctt tcttgatgtg cgggaacgcg accggaaggc
      661 cagagaaatt ttagacgagc tggggatcac gatcgatccc agagccatca tcaaaaacca
      721 gagcgtcgcc cagaaccaac tggtcgaggt ggcgcgggcg gtgtcttatc attccgatct
      781 catcattatg gatgagccga cctcttctct ggcggaagcg gatatccagc tgctctaccg
      841 catcatccgc gggctcaccg ccgagggcgt cgccgtcatc tttatctccc acaagctgga
      901 ggaggtgctg accattgccg accgggtgac cgctatgcgg gatgggaagt acatcgccac
      961 tgcggagacc ggcagctgta cgcaggacga cctgatcaaa tggatcgtag ggcgggatat
     1021 gagcaccatg tatccaaaag agacggcgga gatcggcgac gtggtattgg aggccaggca
     1081 tctcggcaca ttggacaagc gcgtgcggga tgtgagcttt cagttgagga gaggggaaat
     1141 tctcggtttt gccgggctgg tgggcgcggg gcgcacggaa acgatgcggg ccctcttcgg
     1201 catagatcgg ctggagacgg gcgaggtgct gatcgacggc aaaaaggtac ggatcaattc
     1261 gccccgtgaa gcgatcggac tggggctcgg gatggtcacg gaggaccgtc tgcgccaagg
     1321 catcttttcc aagctttcgg tgaggcagaa tatgtcggtg gcttacctga aagacattac
     1381 cggcaggctg ggtctggtgg actttaagac agaggagaga aactgcctgt caatgcggga
     1441 gacactgggg atcaaggtgt ctgcgctcaa acagatgatc tcggggcttt ccggagggaa
     1501 ccagcagaaa gtcatcatcg cgcggtggct gctctcccag ccgcgcatcc tgattttgga
     1561 cgagccgacc cgtgggatcg acgtgggggc caaggcggag attcaccgtc agatcagcct
     1621 gttggcccgc cagggtatgg cgatcattat gatctcctct gagctgcagg aggtactggg
     1681 gatgagcgac cgcatcctgg tgatgcatga gggagagatt gtcggcgagc atctcagggc
     1741 ggatgcggac tctgagcaaa tcatgaaaga agccttcggc gccccggccg aaaggatgga
     1801 aaggcggaat actccatgac cagcgaaaaa agtttgcgtt ctgacaagat aaagcaaagg
     1861 gccatcgagg tgctgatgaa caacaaagcg atgttcatcc tcttagtcct tgtcctcatc
     1921 gccagtgttt tgtcgcccaa cttccttttg ccgcggaaca tttccaacat cttaaaacag
     1981 gtagcggcgg ccaccatcat gggtatgggg tattctctgg tgttgggttc gggccatatg
     2041 gacctgtccg tgggatacct tttggggatg ctgggcatca tcgcagcaaa attggacacg
     2101 cagacctctc tgccgttcgg cctggtggtg gtgatcgtca ttttgatcgg cgctgtgggc
     2161 agtatggcca acgctttctt catcggcgta ttcaccttgc cggcgtttgt ggtcactttg
     2221 gcattggggc aggtctttaa aggcatcaac tatgtcgtca gtaacacctc ggccatttcc
     2281 gggctgtgcg attcttttgt ctttctcgga cagggcagcc tgggccccat ccccctgccc
     2341 gtgctgctgt cggtggcggt aaccctgatc gtctcgctgg tggtctaccg cacggctttt
     2401 gggcggcatt tgttggcggt ggggtccaat ctggaggcgg cgcgggtgtc ggggctcaat
     2461 gtaaagcgga tccgttacgg cgtttattgc ctgatggggg tatgcgtggc ggttgcggcc
     2521 ttggtattga cggggcgatc ggcctccgcg cagccccagt ccgggcaggg catggagatg
     2581 gacgccatcg ccgcggtggt cattggcgga accccgctgg gcggcgggca ttgcaagccg
     2641 gaggggactt ttttcggctg cttaattgtg ggcttgatca acaatatcct caacctgatg
     2701 agcgtcaatt ccaactggca gctggtggcc aagggggcga tcatcctctt cgccatcatc
     2761 ctcgattccc agagcgagaa aatcctggag cgccagaggc tgcgggcgct gaaataagca
     2821 aggcgtatgg ttctccgccc gaaaaggacg ggacggatgc gccgcgaaat gccggcgcag
     2881 gaaaggaaag gacagcatgc tggagtggag accttttgat tatcattcac tggaagaact
     2941 gcagcaggat atccgcgaag ccggagtcaa tctgccgatc agtgaaaact tcaatgcttt
     3001 caaaaaaccg ttggaaattt acgggcatac cctgaaaaat cggctctcca tccaacccct
     3061 ggagggattt gacgcgggag aggacgggac gcccagtgac ctgacctttc gcagatatcg
     3121 aagatttgcc gcagggggcg cggggctgct ctggtttgag tcctgttccc ccaccgccac
     3181 cagccagacc agccgtcggc aatgccgcat cacggacaaa aatctgcctg tatttcaaag
     3241 gctgctgtac gagatacggg acagtgccgc ggacggcgca ccgcctttta ccattgctca
     3301 gctcacgcac tgcggcagaa attcacggtt ggatggagac ggcacttttg gggataaagg
     3361 gctccctcag tatgtggctt gcgacaaccc ctatttgccg aagcgccatg ccgtgatcct
     3421 gagggatgaa gaccttgatc acgttatgga cgactatgtc aaagctgcaa agctgatgaa
     3481 aaaggcaggc tttgacgcag tggatatccg gggctgtcac gggtacctca tatccgagct
     3541 tttgagctct tttacacgag agaacagccg ctatggcgga ccggattttg aaaacaggac
     3601 tcgctttctg ctggaaacca tcgaccgtgt acaggcggag gtggggattc cggtggccat
     3661 ccggctcaac gcctgcgata tgatccctta tccctacggc tggggcatga gccgggacgg
     3721 ttcgctgcgg ccggacctca ccgagccgtt aaggctcggg aaattgctgg cggacagggg
     3781 agtggtcctg ctgaatatca gtatgggccg caaccacgcc acgcagctgc agttgcccta
     3841 taaccgcggc tcacatcggc cgaaagagca tcagctgtac gccatggcct tttaccactc
     3901 tctggcagcg gcttttaaaa aggagattcc ccaggccgtg gtaatgaccg gcaccctctc
     3961 ttggccgggg atctatgcgc cctacatcgc ggctggtgag atggaagctg ggatgtacga
     4021 tctgcccgga tttggaagac taccctgggc ttatcctgat ttcgcccggg atatcctgga
     4081 aaacggcggg ttgaagcgtg aaaaatgctg cattgcctgt aacagctgct gtaatatgat
     4141 cggggttgcc gcctcaaccg gttgtccggt gggctgcccg gtgcgggatc ctgaaattta
     4201 cgcccctctt taccggcagt tcgtcggtgc ctcggtgcgg cagatgtcgc cccagacggt
     4261 ggaattgttt aaattggaga agttggggga gagataaagg gcggtctggg agagcgacca
     4321 ggcctcttga tcaaaaaacg cgaaaatagc aaagcccaag cctcggcgcc gcttttgcgg
     4381 gcgtcggggc ttgggctttg tctcttttac agagaaaaaa gtgacgtttt ttggcttgcc
     4441 cgcatatagt gaaaaaaaga gggcaggggg gtggagggat gcggtaccgg cagaaaaggg
     4501 caaggcaaaa acgccgcaga ttgccctggc tgctgcggct ggccgcggtg ttcgccgtgg
     4561 tggtcggcct gctggtggcg ctggacaacg aggtgcggcc ggtggtgatc acgatggcgc
     4621 agtaccaggc ccgggtcagc agcatcctgg ccatcaacga ggcggtgctc gacgagctgg
     4681 aaaaagagga ggtcggctat gacgacctgg tcacggtcac ccgtctggag gacggcaccg
     4741 tctcctccat acaggccgac accaccgcca tcaaccgcct caaggcgcgg ctgaccaacg
     4801 ccgtcagcgc gcggctgacc gcactgcagc tggaggaggt ggaggtgccg gtgggcacgc
     4861 tgctgggctg gcagatcttt gccgggcgcg ggccggtgat ccgctttcag atcgtgccgg
     4921 cgtcgtttgt ggagagcgac atcaccagca cgctggaggc ggcgggcatc aaccagaccc
     4981 agcacaagat tttgatgcag ttcaaggtgg agatgagcgc catcatcccc gggtatacca
     5041 ccagcgtgac cgtcaacacc caggtcagca tcgcggacac cctgatcgtg ggcaatgtgc
     5101 cgcagtttta cgcggtgggc gacccgttgg catgaccggc cgggtctcat gaattttaga
     5161 caaaaatacc gcccagaata tagccgaagc gccgaagttg tgctatactg aatcagatag
     5221 ggcggtgagt gcatggaaga acatcaggcg cgcgccagaa ttgaagaact gcgcgccgag
     5281 atcagaaaaa acgacgacct gtattataac caggacgccc cggagctgga ggattatgaa
     5341 tacgaccggc tcacccagga gctcaaacgg ctggagcggg agttcccgca gctggtgacg
     5401 gcggattcgc ccacccagaa gatcgacgcc gcggattctt ccgcttttg
//
LOCUS       DBMDYA010000097         5426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3737_length_5426_cov_1.277934, whole genome shotgun sequence.
ACCESSION   DBMDYA010000097 DBMDYA010000000
VERSION     DBMDYA010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_5426_cov_1.277934"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="ACZPFK_03555"
     CDS             <1..580
                     /locus_tag="ACZPFK_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YlqF/YawG family GTPase"
                     /protein_id="HIR1405422.1"
                     /translation="TELAELLERRAVKGMAGAKIRVMVVGIPNVGKSTFINSFAGAAR
                     AKAADRPGVTRGKQWVTAGSYDLLDMPGVLWKKFEDRKTAVNLAFIGSIKDELLDMEE
                     IAAELLSEVKRLYPEKLAERYKLKPAQAEKERYDLLSEIGRNRGMLIAGGEVDLSRAA
                     VTVVDEFRASKLGNLSLESPSGSGGQDAEEGT"
     gene            577..1182
                     /locus_tag="ACZPFK_03560"
     CDS             577..1182
                     /locus_tag="ACZPFK_03560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1405423.1"
                     /translation="MSALYEYDRPLIDRYGLVCGVDEAGRGPLAGPVCAAAVILDEGT
                     VFEELNDSKKLTEKRREALYPRIIENALSYKIVLVGPEVIDEINILQATMKGMREAIE
                     GLTPAPALALIDGNRCPQGLGMPARAVIKGDATSACIAAASVLAKVTRDRYMVELGRR
                     YPQYQFEKHKGYPTKLHYELIGQYGIQDFYRKSFLKKRGIV"
     gene            1182..1541
                     /locus_tag="ACZPFK_03565"
     CDS             1182..1541
                     /locus_tag="ACZPFK_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1405424.1"
                     /translation="MDRKTAGIMGEVAAAKLYRKAGYQLLASNFRTRQGEIDLVAEGQ
                     GCLVFAEVKTREEGGLVTPREAVTPAKQRRIIAAAKGYLEKFGDSGRPIRFDVVEVYY
                     SGDAVTRVEQIENAFTA"
     gene            1555..3012
                     /gene="thrC"
                     /locus_tag="ACZPFK_03570"
     CDS             1555..3012
                     /gene="thrC"
                     /locus_tag="ACZPFK_03570"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1405425.1"
                     /translation="MKYHSTRNASLRRTASQAILQGISEEGGLFVPQEIPQVDLASLQ
                     GLDYCALAAKLLGLYLTDYSADFLLRAARQAYGPQRFGGAAAKTVKLEQGLYSLELWH
                     GPTSAFKDYALQLMPLLLVEAKRINRDDGRTAILVATSGDTGKAALEGYKDLPGVEIA
                     VFYPDHGTSEIQRLQMATQEGANVHVFAVSGNFDDAQTGVKRVFTDAAFAAECAAQGK
                     HLTSANSINWGRLAPQIVYYFASYLDLVRTGELRLGDALDFCVPTGNFGDILAGYYAR
                     RMGLPVRRLICASNRNKVLTDFGASGVYDANRPFYKTASPSMDILVSSNLERLLYHES
                     GDDALVAQLMGALSQNKKYQVPGRLKGRIAEVFTFGCTDEERCFAEIAATWEKDHYLA
                     DPHTAVALSAARVQADGKTPCVVLSTANPYKFSGAVLAALGGSVQADDFANLDALEQL
                     TGTAAPAALRALRQKPVRFEATIRPEEIPAAARAL"
     gene            3024..3704
                     /locus_tag="ACZPFK_03575"
     CDS             3024..3704
                     /locus_tag="ACZPFK_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1405426.1"
                     /translation="MSLYAIADLHLSLGADKPMDIFGGWKDYVPRLEANWNRLVRPED
                     TVVLPGDISWAMKLENAVEDFRFIDRLPGRKIILKGNHDYWWSTMAKMDAFCAAHGFS
                     SIHFLNNNCYWYGETALCGTRSWLFDVGEPHDAKVMNRELCRLRASLQSAGEAEKLVF
                     LHYPPLYPAGNADEVLALLREFGIRECWYGHLHGPSIRGAIQGEVDGVRYKLISADAL
                     GFCPVRVR"
     gene            3804..>4220
                     /locus_tag="ACZPFK_03580"
     CDS             3804..>4220
                     /locus_tag="ACZPFK_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HIR1405427.1"
                     /translation="MELRKNEKLENSRVELEIAVDAAAFSDAVSKAFKKNAKNFNVPG
                     FRKGKAPRHLIEQMYGKDVFHYDAVSEIFPEVYDEAVKAAGLEPVDRPEAELVSASLE
                     DGAVLKVVVTVKPEVELGDYKGLKATKTVHTVDASEA"
     assembly_gap    4221..4230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4231..5099
                     /gene="tig"
                     /locus_tag="ACZPFK_03585"
     CDS             <4231..5099
                     /gene="tig"
                     /locus_tag="ACZPFK_03585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1405428.1"
                     /translation="RTITREGKAENGDIAVIDFEGFVDGVAFEGGKGENFSLALGSGQ
                     FIPGFEEQVVGHEAGEEFDIEVNFPEEYQAKELAGKAATFKIKLHEVKTKELPELDDE
                     FAKDVSEYDTLAELKDSILKRKQEQTDKEDELNVENALVDQVVDGMKAEIPDCMVSNR
                     VDEMVRDFEYRLEQQGLKLEMYLQYTNSTMEKFREGFREQAEKQVKIRLALEKIVELE
                     KIEASEEDFDAEIKRIAAAYQMEEEKIRSVVPAAEVKKDLAVNKAIDFIKSNAVVTEE
                     AAKEEKAEEKND"
     gene            5124..>5426
                     /locus_tag="ACZPFK_03590"
     CDS             5124..>5426
                     /locus_tag="ACZPFK_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR1405429.1"
                     /translation="MSLVPYVVEQTSRGERSYDIFSRLLNDRIIMLSEEITDATASVV
                     VAQLLYLEGQDPDKDISLYINSPGGSITAGMAIHDTMQYIKCDVSTTCIGVAASMAS"
BASE COUNT         1118 a         1580 c         1783 g          935 t
ORIGIN      
        1 gacggaattg gcggagcttc tggagcgccg tgccgtcaag ggcatggcgg gtgcgaagat
       61 ccgcgtgatg gtcgtgggca tccccaatgt gggaaaatca acgtttatca acagcttcgc
      121 gggcgcggcg cgcgcgaagg ctgccgacag gcccggcgtt acgcggggca agcagtgggt
      181 gacggcgggc agctacgatt tgctggacat gcccggcgtg ctctggaaaa aatttgagga
      241 ccgcaaaacg gcggtgaatc tggcttttat cggttcgatc aaggacgaac ttctcgatat
      301 ggaggagatc gcggcggagc tgctgagcga ggtgaagcgc ctctatccgg aaaaactggc
      361 cgagcgctat aagctgaaac ccgcgcaggc ggaaaaggag cggtacgacc tgctgtctga
      421 gatcggccgc aaccgcggca tgctgattgc gggcggcgag gtggacctat cccgcgccgc
      481 cgtgacggtg gtggacgagt tccgcgccag caagctgggg aacctctcgc tggaaagccc
      541 gtccggaagc ggcggtcagg acgcggagga ggggacatga gcgcgcttta tgagtatgac
      601 cgaccgctga tcgaccggta cggcctggtc tgcggcgtgg acgaggcggg gcgcggcccc
      661 ctggcggggc ccgtctgcgc cgcggcggtg atcctggacg agggcacggt gttcgaggag
      721 ctcaatgact cgaaaaagct gaccgaaaag cgccgggagg cgctctatcc caggatcatc
      781 gagaatgccc tcagctataa gatcgtgctg gtggggccgg aagtgatcga cgagattaac
      841 atcctgcagg ccaccatgaa agggatgcgg gaggcgatcg aggggctgac accggccccg
      901 gccctggcgc tgatcgacgg caaccgctgc ccgcaggggc tcggcatgcc cgcgcgcgcc
      961 gtgatcaagg gggatgcgac ctccgcctgc atcgcggcag cctccgtgct ggccaaggtc
     1021 acccgcgacc gctacatggt ggagctgggg cgcagatatc cccagtacca gtttgaaaag
     1081 cacaagggtt accccaccaa gctgcactat gagctgatcg ggcagtacgg cattcaggat
     1141 ttttatcgca aaagctttct caaaaagaga gggattgtct gatggacaga aagacggcgg
     1201 gtatcatggg ggaggtggcg gcggcaaagc tctaccgcaa ggcgggctac cagctgctgg
     1261 cctccaactt ccgcacccgg cagggcgaga tcgacctggt ggcggaggga cagggctgcc
     1321 ttgtctttgc cgaggtgaag acaagagaag agggcggcct cgtcaccccg cgggaggcgg
     1381 tcacccccgc aaaacagcgc cgcatcatcg cggcggccaa gggttatctg gaaaaattcg
     1441 gtgacagcgg ccggcccatc cgctttgacg tggtcgaggt ctattacagc ggcgacgccg
     1501 tcacccgggt ggaacagatc gaaaatgcgt ttaccgcatg agaggggaca gaggatgaag
     1561 tatcacagca cgagaaacgc gtcgctgcgg cgaaccgctt cccaggccat cctgcagggc
     1621 atctccgagg agggcgggct tttcgttcct caggaaattc cgcaggtgga tctggcgtcg
     1681 ctgcaggggc tggactattg cgcgctggcg gcaaagctgc tggggctcta cctgaccgat
     1741 tacagcgctg attttctgct gcgcgccgcc cggcaggcct atgggccgca gcgctttggc
     1801 ggcgcggcgg ccaagactgt aaagctggag caggggctgt acagcctgga gctctggcac
     1861 gggcccacca gcgctttcaa ggattacgcc ctgcagctga tgccgctgct gctggtggaa
     1921 gcaaagcgca tcaaccggga cgacggcagg acggcgattc tggtggcgac ctcgggagac
     1981 accggcaagg ccgcgctgga gggctataag gacctgcccg gcgtcgaaat cgcggtcttt
     2041 tatcccgatc acggcaccag cgagatccag cggttgcaga tggcgaccca ggagggcgcc
     2101 aacgtccatg tctttgcggt cagcggcaat tttgacgacg cgcagacggg ggtaaagcgg
     2161 gtctttaccg acgccgcttt cgccgccgag tgcgcggcgc agggcaagca tctgaccagc
     2221 gccaactcca tcaattgggg ccgcctcgcg ccgcagatcg tctattattt cgccagttac
     2281 ctggaccttg tccgcacggg ggagctgcgg ctgggcgacg cgctggattt ctgcgtgccc
     2341 accggcaatt tcggcgacat cctggccggg tattacgccc gccgcatggg gctgccggtc
     2401 cgccgtctga tctgcgcctc caaccgcaac aaggtgctca ccgatttcgg cgccagcggc
     2461 gtatacgacg ccaaccggcc gttctataag accgcttcgc cctccatgga catcctcgtg
     2521 tcctccaacc tggagcggct gctctatcat gagagcggcg acgacgcttt ggtggcgcag
     2581 ctgatgggcg ccctgtccca gaacaaaaaa tatcaggtgc cgggcagact gaaaggccgc
     2641 attgcggagg tcttcacctt tggctgcacg gatgaggagc gctgctttgc cgagatcgcc
     2701 gcgacctggg aaaaggacca ttatctggcg gacccgcaca ccgccgtggc tctctcggcg
     2761 gcgcgggtcc aggcggacgg caaaacgccc tgcgtggtgc tctccaccgc aaatccctac
     2821 aaattttccg gcgcggtgct cgcggcgctg ggcggcagcg tgcaggcgga tgattttgcc
     2881 aacctcgacg ccctggagca gctcaccggc accgccgccc cggctgccct gcgggccctg
     2941 cggcaaaagc cggtgcgctt tgaggcgacg atccgcccgg aggagatccc ggcggccgcc
     3001 cgcgcgctct gaggggaggc gccatgtcac tttacgccat tgccgacctg catctctcgc
     3061 tgggcgcaga caagcccatg gatatcttcg gcggctggaa agattatgtc ccgcgccttg
     3121 aggccaactg gaaccgtctg gtgcgccccg aggacaccgt cgtgctgccg ggggatatct
     3181 cctgggcgat gaagctggaa aatgcggtgg aggactttcg ctttatcgat cgtctgcccg
     3241 gccgcaagat catcctcaag ggcaaccacg actactggtg gtccaccatg gcgaagatgg
     3301 acgcattctg cgccgcacac ggcttttcca gcatccactt tctcaacaac aactgctatt
     3361 ggtatgggga gaccgccctc tgcggcaccc gcagctggct gttcgacgtg ggcgagcccc
     3421 acgacgccaa ggtcatgaac cgggaactct gccgcctgcg cgcctcgctg cagagcgcgg
     3481 gggaggccga aaaattggtc tttctgcact atccgcccct ttacccggcc ggcaacgccg
     3541 acgaggtgct ggctctgctg cgcgagttcg gcatccggga gtgctggtac ggccatctgc
     3601 acggtccatc gatccgcggc gccatccagg gcgaggtgga cggggtgcgc tacaagctca
     3661 tctccgccga cgcgctgggc ttctgcccgg tgcgggtgcg ctgaaattgc ggctttgcgc
     3721 cgcaggaaag ctcaataaat taatggaaaa ttcatagctg gtatgctata ataaaatgta
     3781 atgtctttta tggaggaaag aaaatggaac tcaggaaaaa tgaaaagctc gagaacagcc
     3841 gcgtggagct ggaaatcgcc gtcgatgccg ccgctttcag cgacgcggtc tccaaggcgt
     3901 ttaagaaaaa tgctaaaaat ttcaatgtcc ccggctttcg caagggcaaa gccccccgtc
     3961 atctgatcga gcagatgtac ggcaaggacg tcttccacta cgacgcggtg agtgagatct
     4021 tccccgaggt ctatgacgag gccgtcaagg ccgccggcct cgagccggtg gaccgtcccg
     4081 aggccgagct ggtctccgcc agcctggagg acggcgccgt gctgaaagtt gtcgtcaccg
     4141 tcaagcccga ggtggagctg ggcgactata agggtttaaa ggccaccaag accgtccata
     4201 ctgtagacgc gtccgaggcc nnnnnnnnnn cccgcaccat cacccgcgag ggcaaggcgg
     4261 agaacggcga catcgcggtg atcgattttg agggctttgt ggacggcgtc gcctttgagg
     4321 gcggcaaggg cgagaacttc tccctggcgc tgggcagcgg ccagttcatc ccgggttttg
     4381 aagagcaggt cgtgggccac gaggcgggtg aggagtttga catcgaggtc aatttccccg
     4441 aggagtatca ggccaaggag ctggcaggca aggccgccac tttcaagatc aagctgcacg
     4501 aggtcaagac taaagagctg ccggagctgg acgacgagtt cgccaaggat gtcagcgagt
     4561 acgacacgct ggccgagctc aaggacagca tcctcaagcg caagcaggag cagaccgaca
     4621 aagaggatga gctcaacgtc gagaacgccc tggtggacca ggtcgtggac ggcatgaaag
     4681 ccgagatccc cgactgcatg gtgtcgaacc gggtggacga gatggtgcgc gactttgagt
     4741 accgtctcga gcagcagggc ctcaagctgg agatgtatct gcagtacacc aacagcacca
     4801 tggaaaagtt ccgcgagggc ttccgtgagc aggccgaaaa gcaggtcaag atccgccttg
     4861 ctctggagaa gatcgtagag ctggaaaaga tcgaggccag cgaggaggac tttgacgccg
     4921 agatcaagcg catcgccgcc gcctaccaga tggaggagga gaagatccgc agcgtcgtcc
     4981 cggccgccga ggtcaaaaag gatctggccg tcaacaaggc cattgacttc atcaaatcca
     5041 acgccgtggt gaccgaagag gccgccaaag aggaaaaggc ggaggagaag aacgactaaa
     5101 atttccattc ggagggtttt gcaatgagtt tggtccctta tgtcgttgag cagacgagcc
     5161 gcggcgagcg gtcttatgac atcttttcac ggcttttgaa cgaccgcatc atcatgttgt
     5221 cggaggagat caccgacgcc accgccagcg tggtggtcgc gcagctgctg tatctcgaag
     5281 ggcaagaccc tgacaaggac atcagccttt atatcaacag cccgggcgga tcaatcaccg
     5341 cgggcatggc gatccacgat accatgcagt atatcaaatg cgacgtctcc actacctgca
     5401 tcggcgtggc ggcctcgatg gcgtct
//
LOCUS       DBMDYA010000098         5411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3751_length_5411_cov_1.533710, whole genome shotgun sequence.
ACCESSION   DBMDYA010000098 DBMDYA010000000
VERSION     DBMDYA010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_5411_cov_1.533710"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            345..1250
                     /locus_tag="ACZPFK_03595"
     CDS             345..1250
                     /locus_tag="ACZPFK_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1405430.1"
                     /translation="MKKFLTLALAAVMALSLAACGGDSSSSAPAGSSAAGSSAAAGGS
                     SAADDGIGMASGNVEEHSLQAIKDRGVLVVTTEAGYAPFEFLDDEGNVVGLDASLAQA
                     LADDLGVELKIENIDFNSVVPEVQAGNADMAIAGLTPNEERKQSVDMSNIYYGGGQCM
                     LVLEKNLEKYATKESLVGETLATQKAALQETLLGEQFPDSTPLLLPKFPQCIAELKNG
                     ACAAVLIDQVSAEQYMKTTEGLAIGKVPVETDPAENGNSVAVMKGNTELLDWVNGKIK
                     EYEEAGKIQQWFDAAKEQAETMGVE"
     gene            1379..2101
                     /locus_tag="ACZPFK_03600"
     CDS             1379..2101
                     /locus_tag="ACZPFK_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1405431.1"
                     /translation="MISGDRIVKIWAKYGHFYLQGLGNTLWVTLLAVSLGVVLGLLVA
                     SGRMMRLRPRDNPVLKVVKAVVRFLCTAYVEVLRATPLIVQVFVIYYSATGIGLSLSD
                     ATATRMMWGIIAVGLNSGAYLSEVIRSGIGAVPGGQLEAARCIGMSQWKAMRFVILPQ
                     AIRNILPALCNEFVTIIKETSVLAMVGIAELMFKAQSISTQTYIFVEPYIIAAAMYFT
                     VVFPLSKLIAAVERRMNKSVTR"
     gene            2088..2825
                     /locus_tag="ACZPFK_03605"
     CDS             2088..2825
                     /locus_tag="ACZPFK_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1405432.1"
                     /translation="MSRDKLIEVKGLEKRFKRLEVLRGVDIDIHKGDVVAVIGPSGSG
                     KSTFLRCLNLLEEPTGGRIIFEGADITSKETNIDVMRQKIGMVFQQFNLFANMTVLDN
                     VMAGPLRVKGMKRDEAEKLALSLLERVGLGDRGSAYPIQLSGGQQQRVAIARALAMQP
                     DVMLFDEPTSALDPEMVREVLDVIRQLAQEGMTMVVVTHEMGFAREAASRVIFMDEGV
                     IMEENGPQEFFDHPQNPRLRDFLSKVL"
     gene            complement(3708..3893)
                     /locus_tag="ACZPFK_03610"
     CDS             complement(3708..3893)
                     /locus_tag="ACZPFK_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405433.1"
                     /translation="MPKSSFEEGFCELLMTPEWQDLEKHLFAAVEMAYAFGWASAGGP
                     LPDMKEKENAPEPENQR"
     gene            4033..4257
                     /locus_tag="ACZPFK_03615"
     CDS             4033..4257
                     /locus_tag="ACZPFK_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405434.1"
                     /translation="MEKKHGDIYARVGLNILYYRKKQGLTQLQLAEAANYSLNHIQQI
                     ETAKTTPSVEALLDIADALEVPVTELFADY"
     gene            4441..5025
                     /locus_tag="ACZPFK_03620"
     CDS             4441..5025
                     /locus_tag="ACZPFK_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1405435.1"
                     /translation="MKNILVILGGGRPNGNTAQLAEAFARGAREAGHRVETVSLLKTE
                     VRGCLGCNACRRGKPCVQRDGFGELVPKIEAADLLVFASPLYFWTFSAKLKALIERFY
                     CLARPEKDPPRGRYEKYPDKDCALLMTAADDLFWTFQQAASYYKFALVEYIGFHDRGM
                     LLAGGCGDTNGGPRIRETPHLDEAYAFGKTIYPA"
BASE COUNT          944 a         1680 c         1806 g          981 t
ORIGIN      
        1 ctcttttccc acgccccctc gcccggcggc gctcctgctg atgggcagcg atgcgccctg
       61 ccggcaaccg cgtcttcgca ccctcccccg gcggcgcgcc gccccccgcc agctctttca
      121 gcctttctcc ctgctccgcc gcccttgtcc ctccacacag cccatcctcc tctcgcccgc
      181 tctctgcctg tcagaatggc caaaacctct gccccgccgc cgcgctggtc ttgccaaaat
      241 gctgaaatgc ataaatatac gttgaaaaag catatttatg catctatact ggggacacag
      301 agaaaaagaa aaaacagaca cagaggtttt tgggaggaat aaagatgaaa aagtttttga
      361 cccttgcgct cgccgcggtg atggcgctct cgctggctgc ctgcggcggc gattccagct
      421 cttcggcccc ggcgggctct tcggccgccg gctcctccgc tgcggcgggc ggctcttccg
      481 ctgcggacga cggcatcggc atggcctccg gcaatgtgga ggagcacagc ctgcaggcca
      541 tcaaggaccg cggcgtgctg gtggtcacca ccgaggcggg ctacgccccg tttgagtttt
      601 tggacgacga gggcaacgtg gtgggccttg acgcctctct ggcgcaggcc ctggcggacg
      661 acctgggcgt ggagctgaag atcgagaaca tcgacttcaa ctcggtggtg cccgaggtgc
      721 aggcgggcaa cgccgacatg gccatcgcgg gcctgacccc caacgaggag cgcaagcaga
      781 gcgtggacat gagcaacatc tactacggcg gcggccagtg catgctggtg ctggagaaga
      841 atctggagaa atacgccacc aaggagagcc tggtgggcga gaccctggcc acccagaaag
      901 cggccctgca ggagactttg ctgggcgagc agttccccga ctccaccccg ctgctgctgc
      961 ccaagttccc ccagtgcatc gcggagctga aaaacggcgc ctgcgcggcg gtgctcatcg
     1021 accaggtcag cgccgagcag tacatgaaga ccaccgaggg cctggccatc ggcaaggtgc
     1081 cggtggagac cgaccccgct gaaaacggca actcggtcgc cgtgatgaaa ggcaacaccg
     1141 agctgctgga ctgggtcaac ggcaagatca aggagtacga ggaggccggc aagatccagc
     1201 agtggttcga cgccgccaaa gagcaggccg agaccatggg tgttgagtaa ggcgggagat
     1261 tgttgtaaaa tagatacgcg gcggagggat gctccttcgc cgctgtattt gtgtcacgca
     1321 tacagcacag gcggggcgga cggctttcag cgggccgccc cgcggcaagg aggacgcaat
     1381 gatttcaggc gatcgcatcg tcaagatctg ggccaaatac ggccactttt acctgcaggg
     1441 cctgggcaac accctctggg tgaccctgct ggccgtctcg ctgggcgtgg tgctggggct
     1501 gctggtggcc tcgggccgca tgatgaggct gcgcccccgc gacaacccgg tgctcaaggt
     1561 ggtcaaggcc gtggtgcgct ttctctgcac cgcctatgtg gaggtgctgc gggccacgcc
     1621 gctgatcgtg caggtgttcg tcatctacta cagcgccacc ggcatcgggc tgagcctgtc
     1681 cgacgccacc gccacccgca tgatgtgggg catcatcgcc gtgggcctca actcgggcgc
     1741 ctatctgtcc gaggtcatcc gctccggcat cggggcggtg cccggcggtc agctggaggc
     1801 cgcgcgctgc atcggcatga gccagtggaa ggcgatgcgc ttcgtcatcc tgccccaggc
     1861 catccgcaac atcctgcccg ccctgtgcaa cgagttcgtc accatcatca aggagaccag
     1921 cgtgctggcc atggtgggca ttgccgagct gatgttcaag gcgcagtcca tctccaccca
     1981 gacctacatc ttcgtggagc cctatatcat cgcggcggcg atgtatttca ccgtggtgtt
     2041 cccgctgtcc aagctcatcg cggcggtgga gaggaggatg aacaagagtg tcacgagata
     2101 aactcatcga ggtcaagggg ctggaaaagc gtttcaagcg cctggaggtg ctgcgcggcg
     2161 tcgacatcga catccacaag ggggacgtgg tggccgtcat cggcccctcc ggcagcggca
     2221 agtccacctt tctgcgctgc ctgaacctgc tggaggagcc caccggcggc cggatcatct
     2281 ttgagggcgc ggacatcacc tccaaggaga ccaacatcga cgtcatgcgc cagaagatcg
     2341 gcatggtgtt tcagcagttc aacctgttcg ccaacatgac ggtgctggac aacgtcatgg
     2401 ccggcccgct gcgggtcaag ggcatgaagc gggacgaggc ggaaaagctg gccctgagcc
     2461 tgctcgagcg ggtgggcctg ggcgaccggg gcagcgccta ccccatccag ctctcgggcg
     2521 ggcagcagca gcgggtggcc atcgcccgcg ccctggccat gcagccggac gtgatgctgt
     2581 tcgacgagcc caccagcgcc ctggacccgg agatggtgcg cgaggtgctg gacgtcatcc
     2641 gccagctggc gcaggagggc atgaccatgg tggtggtgac ccacgagatg ggctttgccc
     2701 gcgaggcggc cagccgggtg atcttcatgg acgagggggt catcatggag gagaacggcc
     2761 cgcaggagtt tttcgaccac ccccagaatc cgcgtctgcg ggattttctc agcaaggtgc
     2821 tgtgaggccc caaagcggac gggagcgggg cgggggaagc gcaggccaaa gcccggtttt
     2881 ttcgggccct gccctgcggg ccgttgcccc cgagagcgcc cggcggcttt gacgttccgc
     2941 gtgtaaaaaa aagggacgac gatctctgtg gatcgccgcc cttttttgtc ctgcatggtt
     3001 tttcttttaa aagcgctttg ccgcagcgca gccggggccg cacgcttaca gccccgcccc
     3061 ggtgtttttg gttttgcgga tttgacccgc ggatttgacc cgcggatttg acccggggat
     3121 gcggctgggg gactcagccg caggggttca gcccagcggc tttgacaccc cgcggccggt
     3181 attggatgaa tggaaaaggg ttggcgttcc gagagggagg ccaacccttt tttgcacggt
     3241 ggtttttcga ggccgccggt ggggaaagcc cgccgctttt gcagcggaga agcccccgca
     3301 gggcagaaaa gcctcgcccc ggcggctggt accgcagcgg gcaggcacgg ccgcgggcgg
     3361 cgcgggcggc cgcatttttg atgcggtcca ctctccgggc agccgcaggc cgatgcgcgg
     3421 cgcagcgggc ccgctggatc tgccgcgctt tcatgcgggg ggcgtttttt cgcgcgggcg
     3481 cgctgggccc ctttctcagt tctggggccg aacgctccgc cccggcgttt tctgcgcggc
     3541 tgtgcggacg ccccggagcc gccttttgcg ggcggggtgc ggacggggcg taggcagaac
     3601 gcggcagagc gtggatgagg tacggacgga atgcggagag aatacaaaca gaactcgggc
     3661 agggccaagt agagtgcgga cgggcccagg cagagcggct gcccgcgtca gcgctgattt
     3721 tctggttccg gggcgttttc tttctctttc atgtcgggca gcggcccgcc cgcggacgcc
     3781 cagccgaaag cgtaggccat ctccaccgcc gcgaacaggt gcttttccag atcctgccac
     3841 tccggcgtca tcaacagctc gcaaaatccc tcttcaaaag aagactttgg catttttaac
     3901 acctcccagc cgagtatacc atggcgggaa aaaaacaaat accaactata agggtgcatt
     3961 tgcaccctgt tggctggtct cccggccgcc ttttttcctg taaaataaag ggagaaaagg
     4021 ggggaggccc gcgtggagaa aaagcacggt gatatctatg cccgcgtcgg gctgaacatc
     4081 ctttattacc gaaaaaagca gggcttgacc cagctgcagc tggcggaagc ggcaaactac
     4141 agcctgaacc atatccagca gatcgagacg gcaaagacaa cgcccagcgt ggaggccctg
     4201 ctggatatcg ccgacgcgct ggaggtgcct gtgacggagc ttttcgcaga ttattagggg
     4261 gccgtcctct gatttttgtc ccgcctttcg ggccgggccg cttttcgcgg cccggccctg
     4321 cctgttgatg cggggcggct gcccgctgtt caaaaagggc gggctgtgtt aaaatcaggg
     4381 cgtggagacg ccgccgggcg gagcggcgtc ccgtcacagt gaaaagggga ggcgcggagt
     4441 atgaaaaata tcctggtgat cctgggcggc ggcaggccga acggcaatac cgcccagctg
     4501 gcggaggcat tcgcccgcgg ggcgcgggag gcgggccacc gggtggagac ggtctcgctg
     4561 ctgaaaacgg aggtgcgcgg ctgcctgggc tgcaatgcct gccgccgggg caagccctgc
     4621 gtgcagcggg acggcttcgg cgagctcgtc cccaagatcg aggcggcgga cctgctggtc
     4681 tttgcctcgc cgctctattt ctggactttt tcggcaaagc tgaaagcact gatcgagcgc
     4741 ttttactgcc tggcccggcc ggaaaaagac ccgccgcggg gccgctatga aaaatatccc
     4801 gacaaggatt gcgcgctgct catgacggcg gcggacgatc ttttctggac ctttcagcag
     4861 gcggcgtcct actataagtt cgccctggtg gagtacatcg gctttcacga ccggggcatg
     4921 ctgctggccg ggggctgcgg cgacaccaac ggcgggccgc gcatccgcga gacgccccac
     4981 ctggacgagg cgtatgcatt cggcaaaacg atctatccgg cttgagcaca ggcggggaga
     5041 ggcaaagcct ctccccgcct gctttttgtc cgggcggcca agcggcgcgc gctgctgttt
     5101 ttcccgccgg gcctcccggc ttgattctga accgccgcgg ccgtgcggct ttgcgtttgc
     5161 cgctgtgcag agccgtgagc attgctgctt tccctgccag gcccctggct tgatcttggg
     5221 ccgccggggc cgcacggcgt tgcgcctgcc tctgtgcagg gcgacgcgca ttgctgcttt
     5281 ccttgtgggg ccccatgggg cttgatcttg ggccgccgag gccgtgcggt tctgcgtttg
     5341 ccgctgtgaa gaccggtgag cattgcttct ctacctgccg ggccccatag ggcttgatct
     5401 tgggccgccg a
//
LOCUS       DBMDYA010000099         5382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3777_length_5382_cov_1.460519, whole genome shotgun sequence.
ACCESSION   DBMDYA010000099 DBMDYA010000000
VERSION     DBMDYA010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_5382_cov_1.460519"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="ACZPFK_03625"
     CDS             <1..1042
                     /locus_tag="ACZPFK_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1405436.1"
                     /translation="VFGAMRGGEFIVNPLTLPSNAHNLDLCLNTMVTGFKSVIDKLEE
                     RPAAISFAFPGPADYPNGIIGGYLPNFPSFRDGVALGPFLREKFGIPVFINNDGDLYA
                     YGEAIGGMLPSVNARLEAAGSAKRFNNLIGYTFGTGFGIGTFINGRLNRGDNSCVETF
                     CLPHKKMPHIIAEDGVAIRAVKRVYGELTGQPDHNLEPKDIFDIAEGKREGDPEAARK
                     AFAEMGEVAGDAMATAATLIDGLIVIGGGITAAAKYIMPALLESMRGKIHTLNGDELN
                     RVQMKIYNLDDEEEFAAFARGEQRSLKVYGTDREVIYDPQKRIGVAISKIGASKAISI
                     GAYTFALNQLDK"
     gene            1052..2023
                     /locus_tag="ACZPFK_03630"
     CDS             1052..2023
                     /locus_tag="ACZPFK_03630"
                     /inference="COORDINATES: protein motif:HMM:NF042587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR1405437.1"
                     /translation="MYPLKFRPILKSLVWGGEKIAPFKHIETTQHNIGESWELSGVRG
                     NESVVANGEYAGRTISELVAQFKDRLIGKRNYARTGNEFPLLIKFIDAKQDLSIQVHP
                     NDELAARRHNGSKGKTEMWYVVQADEGAELLYGFRHPLSPEEFRAHIEAGTLLDDINA
                     VKVRAGDVLFIEAGTLHGIGAGCLVAEIQQNSNSTYRIFDYGRRDKNGNLRQLHIEKA
                     LAVTRLAPPERPVGPVGPEMDCGGYTETVLGLCDYFSTTLLKVRDSVPLCADSKSYHA
                     LLLTHGAAELKYAGGSLPLSAGECVFVPAGCGAYRVSGACELLLTTL"
     gene            complement(2383..4143)
                     /locus_tag="ACZPFK_03635"
     CDS             complement(2383..4143)
                     /locus_tag="ACZPFK_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405438.1"
                     /translation="MTKIRTFLNRHDLSARALWIAALVLAALKLLLCSFQLMYASPDL
                     SPIDDTLMVDLAKSIASGQWLGEYNWLTLGKHSFFALWLALLNKLGLNFLIGGQLLFA
                     AAALLLLGALRPLFKTNLARFFVFGIVLWTPASWAEHSLRAYRDNIYPALVLLAFAGL
                     LGAFVRWREAAGRALPFYIAAGLALGAAWLSHEDNALLLPFLLCAAILYVVFLVSGRA
                     GQLPAKLALLAVPLALWGGCVAAWCGMNQKVYGRFIVSDFTSSEFSDAMGALARACPE
                     DQDRYLLVPRTTRLALYEVSPTLATIEPYLETEDIYNGYGSIERQEIFSGGIHWAIRS
                     AAEEAGYYRDPETARQFYLAVADEVNAACDAGLLPAGGKRSGTFAPFKAEYLGPTLQK
                     FGEQLRILTLYEQTRPTTLLSIARPDQSEDWESFLHCISTKSAVVNTDDPYYSPLQQM
                     AYGVLDSITWLQRVLLWPMLLLAGWWLCRTAAAAVRGIRKKRPPQDLLGGLLLLGFLL
                     SGLLRVAAISYLMAVSFSIKGYLMYLAPACPLLLAFLAYGTAKWLEGPQEEGAGGDPG
                     GLNQRSGRPSPAGRQKDPGR"
     gene            4540..>5382
                     /locus_tag="ACZPFK_03640"
     CDS             4540..>5382
                     /locus_tag="ACZPFK_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1405439.1"
                     /translation="MQYDVVMVTYNSAKWVPGCIQALAKVHSPLENLHLIVVDNASTD
                     DTLEQLTALRKTYLDFGGFTIEKSSRNRGFGAACNLGAQKGSSPLLFFLNLDTEVDPE
                     VFLRLDEAERLHPEAGGFECRQLPYETGHHIDPVTLETSWASGAALAVRRDVFEQVDG
                     FDEHLFMYCEDVDLSWRIRAAGYPLYYVPKAKVNHYSYERAQPKLGEYAGSFYGNLLL
                     RYKFGGWRDILEGHQVYLGALRRPLHFDKVRRVLAVNYLRHFIHLWPFLFWRFAHRGQ
                     YKART"
BASE COUNT         1196 a         1676 c         1491 g         1019 t
ORIGIN      
        1 ggtattcggc gcaatgcgcg gcggcgaatt tatcgtcaac ccgctcaccc tcccgtcgaa
       61 cgcccacaac ctcgacctct gcctaaacac tatggtcaca gggttcaaat ccgtaatcga
      121 taaacttgaa gaacgccctg cggcaatcag ctttgccttt cccggcccgg ccgattaccc
      181 caatggcatc atcggcggct acctgcccaa ctttccgtcg ttccgcgacg gcgtggccct
      241 cggacccttc ctccgcgaga agttcggcat acccgtattc atcaacaacg acggcgacct
      301 ttacgcctac ggcgaggcca taggcggaat gctgccctcc gtgaacgccc gcctcgaagc
      361 ggccggaagc gccaagcgct tcaacaacct cataggctac accttcggca cgggcttcgg
      421 tatcggaacg ttcatcaacg gccggctcaa ccgcggcgac aactcctgcg tcgagacctt
      481 ctgcctgccc cacaagaaaa tgccgcacat catagccgag gacggcgttg ccatacgcgc
      541 cgtcaagcgc gtgtacggcg agctcaccgg ccagcccgac cacaatttgg aacctaaaga
      601 catattcgac attgccgaag gcaagcggga gggcgacccc gaggcggccc gcaaggcatt
      661 cgccgagatg ggcgaagtgg cgggcgacgc aatggccacg gccgcgacgc tcatcgacgg
      721 cctcatcgtc ataggcgggg gaataaccgc cgccgccaag tacatcatgc ccgcgctgct
      781 cgaatccatg cgcggcaaga tacacaccct caacggcgac gagctcaacc gcgtgcagat
      841 gaaaatatac aacctcgacg acgaggagga atttgcggcc ttcgcgcgcg gcgagcagcg
      901 cagcctcaaa gtctacggca ctgacaggga agttatctac gacccccaaa agcgaatcgg
      961 cgtggcaatc tccaagatag gcgcaagcaa ggcaatctcc atcggggcgt acacattcgc
     1021 actcaaccag ttggacaaat aacacaaaga catgtacccc ttaaagttca gacccattct
     1081 caagagcctc gtatggggcg gcgagaaaat agccccgttc aaacacatcg aaaccacgca
     1141 gcacaacata ggcgaaagct gggagctctc gggcgtgcgg ggcaacgagt cggtcgtcgc
     1201 caacggcgaa tacgccggcc gcacaatcag cgaacttgtc gcgcagttca aggaccgcct
     1261 catcggaaag cgcaactacg caaggacggg caacgagttc cccctgctca tcaagttcat
     1321 tgacgccaaa caggacctct caatccaggt gcaccccaat gacgaactag cggccaggag
     1381 gcacaacggt tcaaagggca agaccgagat gtggtacgtc gtgcaggcgg acgagggggc
     1441 cgagctgctc tatggcttcc gccacccgct ttcccccgag gagttccgcg cccacatcga
     1501 ggccggcacc ctgctggacg acatcaacgc ggtcaaggtg cgggcggggg acgtgctgtt
     1561 catcgaggcc ggcaccctgc acggcatcgg cgcgggctgt ctggtcgccg agattcagca
     1621 gaactccaac tccacctacc gcatcttcga ttacgggcgc agggataaaa acggcaatct
     1681 gcgtcagctt catatcgaaa aggcgctggc cgtcacccgg ctcgccccgc ccgagcgccc
     1741 cgtcggcccc gtggggccgg agatggactg cggcggctac accgagaccg tgctgggcct
     1801 gtgcgactat ttttccacca ccctgctgaa agtgcgggac tccgtccccc tgtgtgccga
     1861 cagcaaaagc tatcacgccc tgctgctcac ccacggcgcg gcggagctga aatacgcggg
     1921 cggcagcctg cccctctccg ccggggagtg cgtcttcgtc cccgccggct gtggcgccta
     1981 ccgggtcagc ggggcctgcg agctgctgtt gaccacgctg taagcgcttt ttgtacaaac
     2041 tgcagaggcg aaagcccggg caagggtggg tttccgcaag aaaacattgc cccggagagg
     2101 gaaatttgcg cgcggagcgt cgtcaaatcc atctctcagc cacagaggtg tttgcggttt
     2161 cgccttgcga aatgtctgcg caagcaggcg atctgcgtgc atttttcccg ctccgcatcg
     2221 caacgctcct ttgcgaggga tgtgggcagc ttcgaaccgg cggtcttgtg ctgagcaccg
     2281 cacaaaaaag gttggcaatc cacagagatt gccaaccttt ttgcatctgc agagcgtcaa
     2341 agccaccggt ccagcgccaa tgctctctta cctcaacgca gctcacctgc cggggtcttt
     2401 ttgtcgtcct gcgggggagg gccgtccgct tctctgattc aggccgcccg ggtccccccc
     2461 cgctccctct tcctgcgggc cctccagcca cttggccgtg ccgtaggcca aaaacgccag
     2521 cagcagcggg caggccggcg ccagatacat cagatagccc ttgatggaaa agctcaccgc
     2581 catcagatag gagatcgccg ccacccgcag caggccgctc agcaaaaagc ccagcagtaa
     2641 caggccgccc aacaggtcct gcggcggccg ttttttgcgg atgccgcgca ccgccgcggc
     2701 cgccgtgcgg caaagccacc accccgccag cagcagcatg ggccacagga ggacgcgctg
     2761 aagccaggtg atggagtcca gcaccccgta agccatctgc tgcagggggg aataataggg
     2821 atcatccgta ttgacgacag cggatttggt gctgatacag tgcagaaagc tttcccaatc
     2881 ctcgctctga tccggtctgg cgatcgagag cagtgtcgtg ggccgggtct gctcatagag
     2941 ggtcaggatg cgcaattgct cgccaaattt ttgtagcgtg ggccccagat actccgcttt
     3001 gaaaggggca aaggtgcccg agcgcttgcc gcccgcgggc agcaggcccg cgtcgcaggc
     3061 cgcgttcacc tcatccgcca ccgcgagata aaactgtcgc gccgtttccg gatccctgta
     3121 atagcccgcc tcctctgcgg cagaccggat ggcccaatgg attccccctg aaaagatctc
     3181 ctgccgctcg atactgccat agccgttgta aatgtcttcc gtctccaaat agggctcgat
     3241 cgtggctaac gtgggggaaa cttcgtataa agcgagccgc gtcgtgcgcg gcaccagcag
     3301 ataccggtcc tgatcctctg ggcaggcgcg ggcgagcgcc cccatcgcgt ccgaaaactc
     3361 actggaggtg aaatcgctga cgataaagcg gccgtagacc ttttggttca tgccgcacca
     3421 ggcggccacg cagccgcccc acagcgccag cggcaccgcc aacagcgcca gctttgccgg
     3481 caattgcccc gcgcggccgg atacgagaaa cacgacgtag aggatcgccg cgcagaggag
     3541 aaagggcagc agcagcgcat tgtcctcgtg cgacagccac gccgccccca gcgccaggcc
     3601 tgcggcgata taaaagggca gcgcccggcc cgccgcctcg cgccagcgca caaaagcccc
     3661 cagcagaccg gcaaaggcca gcagcaccag ggcggggtaa atattgtccc gataggcgcg
     3721 caggctgtgc tccgcccagg aggcaggggt ccacagcacg atgccaaaga cgaaaaagcg
     3781 cgccagattc gttttgaaca gcggccgcag cgcccccagc agcagcaggg cggccgccgc
     3841 aaacagcagc tgtcccccga tgagaaagtt gaggccaagc ttgttcaaaa gggcgagcca
     3901 gagcgcaaaa aatgaatgct tgcccagcgt cagccagttg tactcgccca gccattgccc
     3961 cgaggcaata ctttttgcca gatccaccat cagggtatcg tcgatggggg aaagatcagg
     4021 cgacgcatac atgagctgaa aactgcagag caggagcttg agcgccgcca gcactagagc
     4081 cgcgatccaa agcgcccggg cgctgagatc atgacgattt aaaaacgttc ttatctttgt
     4141 caaaatacca gttcctctct gtcttgaagt tctacttttg cagttgcttt ttatgaaaga
     4201 tcctgtcttc tcatcgctgt cgcggcagct gaaaagatta ttgaaaaata gcctgcggct
     4261 cttttttatt atactctatt ctccacacgg agaaaagcaa ttatgttttc cgtcgatctc
     4321 ccgtcgttat gccccacctc cgccaaccga gcttttggtg cagaatgttt tcgtgtccgc
     4381 tccacgacga acctggccga ccgcctgaca tctcatcttg atctcataaa agtgagatac
     4441 ggcaactttt ttcagtttcc catactggaa ctcaagactt tatggtacaa taagcaacat
     4501 agaacggatc ctattccatg ctacaaagga gaccttactg tgcagtatga cgttgtaatg
     4561 gtgacctata atagtgcaaa gtgggtgccg ggctgtatcc aagccctggc caaagttcac
     4621 tctcctctcg aaaacctgca tctcatcgta gtggacaacg catctaccga tgatacgctg
     4681 gaacaactca ccgctctgcg caagacatat cttgattttg gtggtttcac aatagaaaaa
     4741 agctcccgca atagggggtt cggcgcggct tgcaatttag gggcgcaaaa aggaagttcc
     4801 ccgttacttt tttttctcaa tttagataca gaggtggatc cggaagtatt tctccggctg
     4861 gatgaagcgg agcgcctgca tccagaagcc ggcgggtttg agtgccgtca gcttccttat
     4921 gagaccggac atcacatcga ccctgtgacg ctggagacca gctgggcaag cggcgcagct
     4981 ctggctgttc ggcgcgatgt ttttgaacag gtcgacggtt ttgacgaaca tctttttatg
     5041 tattgtgagg atgtcgacct ttcctggcgc atccgcgcgg ccggatatcc tctctactat
     5101 gtgccgaaag ccaaagtcaa tcactattct tatgaaagag cccagccaaa attaggcgaa
     5161 tatgccggca gtttttacgg taacctttta ctgcgctaca agttcggcgg ctggcgtgac
     5221 attctggaag ggcaccaggt atatttgggt gcattgcgtc gtcccctgca ttttgataag
     5281 gtacggcgcg ttctcgccgt caactatctt cgacatttta tccacctctg gccatttcta
     5341 ttctggcgtt ttgctcatcg agggcaatac aaagcccgta ca
//
LOCUS       DBMDYA010000100         5352 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3799_length_5352_cov_1.657018, whole genome shotgun sequence.
ACCESSION   DBMDYA010000100 DBMDYA010000000
VERSION     DBMDYA010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_5352_cov_1.657018"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="ACZPFK_03645"
     CDS             <1..274
                     /locus_tag="ACZPFK_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405440.1"
                     /translation="GGLLAAKEQGSALFPPAKQAARSCLGRLARRFFPPAATRGGSGG
                     GSREKPRFQAKKPLFLPLFFHKVLLAFPPCIKYNNAMLVGKSGEKW"
     gene            308..727
                     /gene="mraZ"
                     /locus_tag="ACZPFK_03650"
     CDS             308..727
                     /gene="mraZ"
                     /locus_tag="ACZPFK_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1405441.1"
                     /translation="MLIGTYNQKLDEKGRLNFPVRFRDEMGGQFYVTCWLDECLIALP
                     AAKFEEIFSRLTESGMVKNRGLRRMLYAGAVEAAPDKQGRILLPPPLREHAGLDKEVV
                     VIGVGDYAELWEPARWKAMQDSMRSGDMAAAMEELDF"
     gene            760..1683
                     /gene="rsmH"
                     /locus_tag="ACZPFK_03655"
     CDS             760..1683
                     /gene="rsmH"
                     /locus_tag="ACZPFK_03655"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1405442.1"
                     /translation="MEFSHIPILLDECLQGLAIRPEGVYLDGTAGGAGHAREIAKRLC
                     GGWLYAMDQDPDAVAAATERLAGLPATVVKGNFRHAKELLAAQGVTRLDGALLDLGVS
                     SHQLDDRERGFSYLGDAPLDMRMSQSGPTAADLVNTLERDELARILKEYGEEPYAWQV
                     AGKIVAARQQAPLETTGQLAAAVAAAMPPAVRRKDKNPARRSFQAIRIAVNGELDALS
                     EGLDAIFDLLAPGGRFCILTFHSLEDRLVKQRFRKWCTACTCPPEFPVCVCGGVAKAK
                     QITHKPLQADEREQQQNRRSRSAKLRVIEKL"
     gene            1809..2270
                     /locus_tag="ACZPFK_03660"
     CDS             1809..2270
                     /locus_tag="ACZPFK_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405443.1"
                     /translation="MQQAAYDLSTFENREERPRLRVARGAKPKRKFDFSGLKIVATVA
                     VIFSLAWGVLYSRAQITELTTQISAAENELSDAKSEYDYLNLQLESKTDLESVENYAI
                     TQLGLTKMDASQITYLTMENEDKIVKPESEVKTFFENFSSGFMNVMEYLAP"
     gene            2409..4658
                     /locus_tag="ACZPFK_03665"
     CDS             2409..4658
                     /locus_tag="ACZPFK_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1405444.1"
                     /translation="MSDRQAPQPNMNTVIRRRILALVAVLILLGFGSVVYSLVDLQII
                     EYEDYKVKAANQQLKDTVIPANRGVIYDANMKVLAQSATVWTVAVAPSEIDEKDRAGI
                     AAGLSEILGVDEQSVLDKLAKTKSSYQTIKLKVEKPVADAVRQFAIEGNGGKGYKGIS
                     LSQDSKRYYPYGNFAATVLGFTGTDNNGLSGLEAYYDDVLSGTPGRQVSAQTATGGDM
                     GYEYDSFYAPQDGNSLVLTIDEVIQHYAEKELDRAVQDYNALARGVVIVMDVHTGGIL
                     AMATKGDYDPNNPFTIFDEAKRAEVDAVADDPATEDVDEHKQALLQAQQTQWRNKAVS
                     DLYEPGSVFKLITASAALDSGSSTLNDSFNCTGSIKVAGTTMRCAHTEGHGAENFSQA
                     LVNSCNPAFIQIGTKMGAGTFFNYFNAFGLTKKTGIDLPGEAQSQFYTAEGLGPVQLA
                     SCSFGQSNKITPIQMVTAVATIANGGDLVQPHLVSKVLDASGNLVEDLEPEAKRQVIS
                     EETAKTICSIMQEASGRAAVKGYRVGGKSGTSQKLDSGDPEARISSFVGIAPCDDPQI
                     AVLVILDEPQNKVLGEVFGGYLAAPVVGQIMSQALPYLGVEKVYTAEEAAEADVTVPN
                     VTGQEVANAQVTLTRDKGLSVDVKGNGSTVVRQFPSSGTLPRGGTVTLYTEEGLADEM
                     VTVPEVTGRDMASVEQALAAAGLNLRKDGATGSSAVIATTQDLAAGTQVPLGTVVTVS
                     FHDPGVAAD"
     gene            4705..>5352
                     /locus_tag="ACZPFK_03670"
     CDS             4705..>5352
                     /locus_tag="ACZPFK_03670"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1405445.1"
                     /translation="MELEQLFEGVPYEGELPGGDVTLVTLDSRRAKPGAVFVCTRGGS
                     ADGHSFAPAALESGAALVVTDHRLGLAREVVVADTRRAYALLCHNYFGNPARSLKLVA
                     VTGTNGKTTITYLLKQILEKAGCRCGLIGTIRSEVGDMEIPAKFTTPEAWDLAALFSR
                     MVNAGCSHVVLEASSQALDQLRLYGLQFEVGVFTNLTQDHLDYHKTMENYYLAKRS"
BASE COUNT         1060 a         1680 c         1785 g          827 t
ORIGIN      
        1 gggaggcctt ttggcggcaa aagagcaggg gagcgccctg ttccctccgg caaaacaagc
       61 ggcgcgcagc tgtttggggc ggcttgcgcg ccgctttttt ccacccgccg ccacccgcgg
      121 cggcagcggt ggaggcagcc gcgaaaaacc gcgatttcaa gcgaaaaaac cacttttcct
      181 cccgctcttt tttcacaaag ttcttcttgc attcccaccc tgtatcaagt ataataatgc
      241 tatgttggtg ggtaaaagtg gggagaagtg gtgacctgcc gcctttctcc gaaagggggc
      301 gggctgcttg ctgatcggga cctataacca gaagctggat gaaaagggca ggctcaactt
      361 tcccgtccgc ttccgcgacg agatgggcgg ccagttctac gtcacctgct ggctcgatga
      421 atgcctgatc gccctgcccg ccgccaagtt cgaagagatc ttttcccgcc tgaccgagag
      481 cggcatggtc aaaaaccgcg gcctgcgccg catgctctat gccggcgcgg tggaggcggc
      541 gccggacaag caggggcgca tcctgctgcc gccgccgctg cgggagcacg cgggcctgga
      601 caaagaggtc gtggtcatcg gcgtgggcga ttacgccgag ctctgggagc ccgccagatg
      661 gaaagcgatg caggacagca tgcgcagcgg cgacatggcc gccgccatgg aggagctgga
      721 cttctgataa aggggacggc gagaggaggg gacaagccca tggaattttc tcatatcccc
      781 atcctgctgg acgagtgcct gcaggggctc gccatccggc cggagggcgt ttatctggac
      841 ggcaccgccg gcggcgccgg ccacgcgcgg gagatcgcga agcgcctttg cggcggctgg
      901 ctctacgcca tggatcagga cccggacgcc gtcgcggccg ccaccgagcg gctggccggc
      961 ctgccggcca cagtggtgaa gggcaatttc cgccatgcga aggagctgct cgcggcgcag
     1021 ggggtcaccc ggctggacgg ggcgctgctg gacctggggg tctcgtccca ccagctggac
     1081 gaccgggagc ggggcttttc ctacctgggg gacgcgccgc tggatatgcg catgagccag
     1141 agcggcccca ccgcggcgga ccttgtaaac acgctggagc gggacgagct ggcgcgcatc
     1201 ctcaaggagt acggcgagga accctacgcc tggcaggtgg cgggcaagat cgtggccgcc
     1261 aggcagcagg cccctctcga gaccaccggg cagctggccg cggccgtggc cgcggcaatg
     1321 cctcctgcgg tccgccgcaa ggacaaaaac ccggcgcggc gcagctttca ggcgatccgc
     1381 atcgcggtga acggcgagct ggacgccctc agcgaggggc tggacgccat cttcgacctg
     1441 ttggcgccag gggggcgctt ttgcatcctc acgttccact cgctggagga ccggctggtc
     1501 aagcagcggt tccggaagtg gtgcaccgcc tgcacctgcc cgccggaatt cccggtctgc
     1561 gtgtgcggcg gcgtggcgaa agcaaaacag atcacccata agcccctgca ggcggatgag
     1621 cgcgaacagc agcagaaccg ccgctcccgg tcggcgaagc tgcgggtcat cgaaaagctt
     1681 taggcttcta tccgacgccc cataaagcgg gcgcgtttta aaaaatccgc accgtgcgaa
     1741 ggaaacgatc ccatggcaaa gaaaaacaca gacggcagac aatctgatgg aaaggagggc
     1801 ccgaaaaaat gcagcaggcc gcttatgatc tgagcacttt tgaaaacagg gaagagcgtc
     1861 cccgcctgcg ggtggcgcgc ggcgccaagc ccaagcggaa gttcgatttt tcgggcctca
     1921 agatcgtcgc caccgtggcg gtgatcttct cgttggcctg gggggtgctg tactcccgcg
     1981 cgcagatcac cgagctcacc acccagatct ccgcggcgga gaacgagctc tcggacgcca
     2041 agagcgaata tgattacctg aacctccagc tcgagagcaa gaccgacctg gagtcggtgg
     2101 agaactacgc gatcacccag ctgggcctga ccaagatgga cgccagccag atcacctatc
     2161 tcaccatgga aaacgaggat aagatcgtca agccggagag cgaggtcaaa actttcttcg
     2221 agaatttttc atccggcttc atgaacgtga tggaatacct tgcgccgtga caaaatagga
     2281 tagagtatcg ggccgcgctg tgccgcggcc tgtgatacgg aaggacggaa aggggtaaaa
     2341 cgggtgttta cccctttttc tttcccgata agggaattca aaattcgacg gaaaaggagg
     2401 cggcagccat gtccgaccgg caggccccgc agcccaatat gaataccgtg atccgccgcc
     2461 gcatcctggc gctggtggcg gtgctgatcc tgctggggtt cggcagcgtc gtctacagcc
     2521 tggtggacct gcagatcatc gagtacgagg actataaggt aaaagccgcc aaccagcagc
     2581 tgaaagacac cgtcatcccg gccaaccggg gcgtgatcta cgacgccaac atgaaagtgc
     2641 tggcccagag cgccacggtg tggaccgtgg ccgtggcccc cagcgagatt gacgaaaagg
     2701 accgggcggg catcgccgcc ggcctctccg agatcctggg cgtcgacgag cagtcggtgc
     2761 tcgacaagct ggccaagacc aaaagctctt accagaccat caagctcaag gtggaaaagc
     2821 cggtggcgga cgcggtgcgc cagttcgcca tcgagggcaa cggcggcaag ggctataagg
     2881 gcatcagcct gtcgcaggat tcaaagcggt attaccccta cggcaacttt gccgccacgg
     2941 tgctgggctt taccggcacc gacaacaacg gcctttcggg gctggaggcc tactacgacg
     3001 acgtgctgtc cggcacgccg gggcggcagg tgtcggcgca gacggccacc ggcggggaca
     3061 tggggtatga atacgacagc ttttacgcgc cccaggacgg caacagcctg gtgctcacca
     3121 tcgacgaggt gatccagcac tacgcggaaa aagagctgga ccgcgccgtg caggactaca
     3181 acgcgctggc gcgcggcgtg gtgatcgtca tggacgtgca caccggcggc attctggcga
     3241 tggcgaccaa gggggattac gaccccaaca atccgttcac catctttgac gaggccaagc
     3301 gggccgaggt ggacgccgtg gcggacgacc cggccaccga ggatgtcgac gagcacaagc
     3361 aggccctgct gcaggcgcag cagacccagt ggcgcaacaa ggcggtctcc gatttgtacg
     3421 agccgggctc ggtcttcaag ctcatcaccg cctcggcggc gctggattcc ggctcctcca
     3481 cgctgaacga cagcttcaac tgcaccggct ccatcaaggt ggcgggcacc accatgcgct
     3541 gcgcccacac cgaggggcac ggcgctgaaa acttttccca ggcgctggtc aactcctgca
     3601 acccggcctt tatccagatc ggcaccaaga tgggcgcggg cactttcttc aattatttca
     3661 acgctttcgg gctcaccaaa aaaaccggca tcgacctgcc cggcgaggcg cagagccagt
     3721 tctacaccgc cgagggcctg gggccggtac agttggccag ctgctcgttc gggcagtcca
     3781 acaagatcac ccccatccag atggtcaccg ccgtggccac catcgccaac ggcggcgacc
     3841 tggtgcagcc gcacctggtc agcaaggtgc tggatgcgag cggcaatctg gtggaggacc
     3901 tggagccgga ggccaagcgg caggtcatca gcgaggagac ggcaaagacc atctgctcca
     3961 tcatgcagga ggcctccggc cgcgcggcgg tcaagggcta ccgggtgggc ggcaaaagcg
     4021 gcacctcgca gaagctggac tcgggcgacc ccgaggcgcg catctcgtcg tttgtgggca
     4081 tcgccccctg cgacgacccg cagatcgcgg tgctggtcat cctcgacgag ccccagaaca
     4141 aggtgctggg cgaggtgttc ggcggctatc tggccgcgcc ggtggtgggc cagatcatga
     4201 gccaggcgct gccgtacctg ggcgtcgaaa aggtctacac cgccgaggag gctgcggagg
     4261 ccgacgtcac ggtgcccaac gtcaccgggc aggaggtcgc taacgcccag gtcacgctga
     4321 cccgcgacaa gggcctgtcg gtggatgtca agggcaacgg cagcacggtg gtgcgccagt
     4381 tccccagcag cggcacgctg ccgcggggcg gcacggtcac cctgtatacg gaagagggct
     4441 tggccgacga gatggtcacg gtgccggaag tcaccgggcg cgatatggcc tcggtggagc
     4501 aggcgctggc cgccgcgggg ctcaacctgc gcaaggacgg cgccaccggt tcttcggcgg
     4561 tgatcgccac cacgcaggac cttgccgccg gcacccaggt gccgctgggc acggtcgtca
     4621 ccgtcagctt ccacgacccc ggcgtggcgg cggactgagc gcgccgcatg gcggactgag
     4681 cgcacaggag agaggagagg gcagatggag ctagagcaac tttttgaggg ggtcccctac
     4741 gagggggaac tgccgggcgg cgacgtcaca ctggtcaccc tcgactcgcg cagggcaaag
     4801 ccgggcgcag tcttcgtctg cacgcgtggg ggctccgccg acgggcactc gtttgcaccg
     4861 gccgcgctgg agagcggcgc ggcgctggtg gtcaccgacc accgtctggg cctggcccgg
     4921 gaggtggtgg tggcggacac cagacgcgcc tacgcgctgc tctgccacaa ctatttcggc
     4981 aacccggccc gcagcctcaa gctggtggcg gtgaccggca ccaacggcaa gaccaccatc
     5041 acctatctgc tcaaacagat tttggaaaag gcgggctgcc gctgcggcct gatcggcacc
     5101 atccgcagcg aggtggggga catggagatc ccggccaagt tcaccacccc agaggcatgg
     5161 gacctggcgg ccctgttctc ccgcatggtg aacgcagggt gcagccacgt ggtgctggaa
     5221 gccagcagcc aggcgctgga tcagctgcgg ctttacgggc tgcagtttga ggtgggcgtc
     5281 ttcaccaacc tcacccagga ccacctggat taccacaaga ccatggagaa ttattacctc
     5341 gccaagcgca gc
//
LOCUS       DBMDYA010000101         5303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3844_length_5303_cov_1.684737, whole genome shotgun sequence.
ACCESSION   DBMDYA010000101 DBMDYA010000000
VERSION     DBMDYA010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_5303_cov_1.684737"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="ACZPFK_03675"
     CDS             complement(<1..539)
                     /locus_tag="ACZPFK_03675"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1405446.1"
                     /translation="MQLRNRKARFGTVFLLGLITALLFILPWIFKDGFLYLGFDYVEQ
                     QIPFNQICNRAVKEGTLLWNSSLDLGTGFLEAYSFYVLGSPFFWLSLIFPADWTYVTV
                     GLMLAFKLAVGAVTAYAWLRTYSKTEDWAMVGGLLYTFSGYQISNLNFNHFADVTALF
                     PLLLLALDQTVKLRKKLPLG"
     gene            complement(903..1481)
                     /locus_tag="ACZPFK_03680"
     CDS             complement(903..1481)
                     /locus_tag="ACZPFK_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1405447.1"
                     /translation="MIYLQLFYEFFKAGLFAVGGGLATLPFLYDISDATGWFTHAQLA
                     DMVAVSESTPGPIGINMSTYVGYTTGGIPGAVIATLGLIAPSIIVILIVAGFLRAFKD
                     NRYVKAAFYGLRPASTGLITAACWSLVAISLLRLNAFSAGGLLAVFNWKALLLAAALL
                     ALTHVKKLKDLHPAAFLALSAVVGVVFHFGGA"
     gene            complement(1478..2023)
                     /locus_tag="ACZPFK_03685"
     CDS             complement(1478..2023)
                     /locus_tag="ACZPFK_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1405448.1"
                     /translation="MRELLDLFLTFARIGGLTFGGGYAMLPMLQKEVVEQRGWVTEDE
                     LMDYYAIGQCTPGIIAVNTATFVGQKRRGMVGGVVATLGVVFPSLIIITIIAAFISNF
                     ADLALVKNAFAGIRVCVCVLVFNAILKLLKKSVVDKPTLALYVAVALLAICTDLSPIW
                     FVLAAGLAGVLIQSRKGGAAK"
     gene            2190..2642
                     /locus_tag="ACZPFK_03690"
     CDS             2190..2642
                     /locus_tag="ACZPFK_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR1405449.1"
                     /translation="MAGKCAPRENLPPGAHYQLDGQGLQLVERAAAPGTPPEAQVEKI
                     PAREGEKLLLFNPSDIDYAESADGRSLVAVRGSCFACPLTLEELSARLERYGFFRCHR
                     SYLVNMQKVEEVIRWTRNSYALRVKGGPEGGVPLSKGRVEEMKALYRF"
     gene            2904..3749
                     /locus_tag="ACZPFK_03695"
     CDS             2904..3749
                     /locus_tag="ACZPFK_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405450.1"
                     /translation="MEEQITMRGVGISFGGAQALRDLTFSVGRGEIFGFLGPSGAGKT
                     TTIKLLTRQLVADEGEIRVFAEPVERLAGDAFRRIGVLSDNSGIYERLTVQQNLELFA
                     RLRGVDKAQVQALTEAVGLADARGKTAKKLSRGMRQRVMLARAVLHRPELLFLDEPTA
                     SLDPGTTAEIHKLLRQLNAQGTTIFLTTHDMEEADKLCGRVAFLHHGHIEECGAPEQL
                     KLKYAENEIVALLADGRRLSAEKTVAGLEELRRAAGECAILSIHSQEPDLEEIFLQVT
                     GRKLA"
     gene            3746..4465
                     /locus_tag="ACZPFK_03700"
     CDS             3746..4465
                     /locus_tag="ACZPFK_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405451.1"
                     /translation="MKTHCTRTWVLLRKDAADMVKNPNMLLMLLLPLLFAWIYTFIGA
                     ETGDGLPPVMVLTISTLMALALVPLSLLSSAIAEEKEKNTLRTLMLTGVTAGEFLTAK
                     VLATELAMAAVGAGVFLVAGAPLPVLIPYLAAILLGGLPLLLLGAVIGIASRDQMSTG
                     IAAAPLALLLMIPTIFSAVGGVMGWIAKLTPTTAVLELVYAAMGLQEQSVAYSVAVLV
                     VWSVLTAAAFALIYKKKRLDN"
     gene            4767..>5303
                     /locus_tag="ACZPFK_03705"
     CDS             4767..>5303
                     /locus_tag="ACZPFK_03705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1405452.1"
                     /translation="MLLSQKELGQAAPLFAGWEETMIWSCLEDAMGRVFAAGRPVCSA
                     AARIADFCFLAGRPDAALLREAAGRAALLVPRDEAWAGLIEREWGAGVRRFERYATRK
                     EPRAFDRPRLRGFAGQLPPGCRILPIGEALYHRLAEADWSRDFCGQFPGWPAYQGRGL
                     GFAALCEGKPVSGASSYTV"
BASE COUNT         1095 a         1509 c         1760 g          939 t
ORIGIN      
        1 cctaagggga gttttttgcg tagttttacg gtttgatcta gggccaaaag taaaagaggg
       61 aaaagggccg ttacgtctgc gaaatggttg aagttgaggt tagatatttg atagccggag
      121 aaagtataaa gcaggccgcc caccatagcc cagtcttcgg tcttgctata ggtacggagc
      181 caagcgtagg cggtcactgc accgaccgcg agtttaaacg cgagcatcag ccctacggtc
      241 acgtatgtcc agtcagctgg gaaaataagg cttagccaga agaaaggaga acccaagaca
      301 taaaaagaat aggcctctaa aaagcctgtg cccaaatcaa gactggagtt ccacaacaag
      361 gttccttctt tgacggcacg gttacaaatt tgattaaaag ggatttgttg ttccacatag
      421 tcgaagccca agtagagaaa gccatcttta aaaatccagg gtaaaataaa gagaagagcg
      481 gtgatcaatc ccaggagaaa tacagtgcca aatcgcgctt tccgatttct aagttgcaag
      541 gggagcctcc tatttcaata tttcaaacaa acagtcagta tagaaacagc ttttccatta
      601 taaccacttt ttaacaaaag aacaatcagt acaaggcctt taaaaatgtg aagattaggg
      661 gtggggaaat ttttaaaatt tcatgatttg agaatacaaa agttgatttt cagatttctg
      721 gcagtaaatc aagagatatg tctgaatccg aaattgagta taaaattaga acatagcttt
      781 tgaccagaga aaaaacaagc gatagtatga caactaaaaa cgtctcctct ttccaggagg
      841 agacgtaccg ctcaaaattt ttttatcctc attagacaaa aacgagatca ttttttaatt
      901 cttcacgccc cgccgaaatg gaacacgacg cccactacgg ccgagagagc cagaaaagcg
      961 gcggggtgca ggtccttgag ctttttgacg tgggtgagcg ccagcagcgc ggccgccagc
     1021 aaaagggctt tccagttgaa gaccgccagc aggccgcccg ccgaaaaggc gttcaggcgc
     1081 agcagcgaaa tggcgacaag cgaccagcag gcggcggtga tgagcccggt ggaggcgggg
     1141 cgcaggccgt aaaaggccgc cttgacatag cggttgtctt taaaggcgcg caaaaagccc
     1201 gccacgatga ggatgacgat gatggagggg gcgatgagcc ccagcgtggc gatcaccgcg
     1261 ccggggatgc cgccggtggt gtagcccacg taggtggaca tgttgatgcc gatggggccg
     1321 ggggtggatt cagagacggc caccatgtcc gccagctggg cgtgggtgaa ccagccggtg
     1381 gcgtccgaga tgtcgtagag gaacggcagg gtggcgaggc cgccgcccac cgcaaagagg
     1441 ccggctttga aaaactcgta aaacagctgc agatagatca tttggcggcg ccccctttcc
     1501 tgctctggat cagcacgccc gccaggccgg cggccaggac gaaccagatg ggggagaggt
     1561 cggtgcagat cgccagcagg gccacggcca catagagcgc cagcgtgggc ttgtccacca
     1621 ccgatttttt gagcagcttc aagatggcgt tgaacaccag cacgcagacg cagacgcgga
     1681 tgcccgcaaa ggcgtttttg accagggcca ggtcggcaaa attgctgatg aaagcggcga
     1741 tgatggtgat gataatcaga gaggggaaca ccacccccag cgtcgccacg acgccgccca
     1801 ccatcccgcg gcgcttttgg cccacaaagg tggcggtgtt gacggcgatg atgccgggtg
     1861 tgcactggcc gatggcgtaa tagtccatca gctcatcctc ggtgacccag ccgcgctgtt
     1921 ccacgacctc cttttgcagc atcggcagca tggcgtagcc gccgccaaag gtgaggccgc
     1981 cgatccgcgc aaaggtcaga aaaaggtcga gcaattctct catgaaatcc ctccgctccg
     2041 cgccaaaggc gctgtcttcc cgccgggggc gggcccaaaa ccacaatctc agtatagcat
     2101 ggttcttttc atatgaaaaa tatttgtttg gtatatcttc cataggatag aactataggt
     2161 gtggacgctg ggccccgggc cgtggtaaaa tggccgggaa gtgtgcgccc cgggagaacc
     2221 tgccgccggg ggcccactac cagctggacg gccagggcct gcagctggtg gagcgcgccg
     2281 cggccccggg cacgccgccg gaggcgcagg tggaaaagat accggcgcgg gagggggaaa
     2341 agctgctgct cttcaacccc tccgacatcg actacgccga gagtgcggac ggccgcagcc
     2401 tggttgcggt gcggggctcc tgcttcgcct gtccgctgac gctggaggag ctgtccgccc
     2461 ggctggagcg gtacggcttt ttccgctgcc accgctccta tctcgtcaac atgcaaaagg
     2521 tggaggaggt catccgctgg acccgcaaca gctatgcgct gcgggtaaag ggcgggccgg
     2581 agggcggcgt gccgctgagc aagggcaggg tggaggagat gaaagccctc tatcggttct
     2641 gacccgcccg cctccccatc tgcgcactgt gcgctgggga aaagcagcgc gcgcccgccc
     2701 gtgccgtgca gagaaaaaaa cgatgcgccc gaccgacctg tcccgccagg ggaaaaagag
     2761 tggggcgctg ctccattcgc accccgcagc gcaccgttcg cggcattttt gtggaagccc
     2821 cgggatggaa tgagttaaaa tagccagggc cggaggggag aagcagcttc ccaaacaaga
     2881 gccggccgga ggagttatca gagatggaag aacagatcac catgcgtggc gtcggcattt
     2941 cgttcggcgg cgcgcaggcc ctgcgcgacc tcaccttttc ggtggggcgg ggcgaaatct
     3001 ttggcttttt gggcccgtcg ggcgcgggca agacgaccac catcaagctg ctgacccggc
     3061 agctggtggc ggatgagggg gagatccggg tcttcgccga gccggtggag cggctggcgg
     3121 gggacgcttt tcgccgcatc ggggtgctca gcgacaacag cggcatctac gagcggctca
     3181 ccgtgcagca gaatctggag ctgtttgccc ggctgcgggg cgtggacaag gcccaggtgc
     3241 aggccctgac cgaagcggtc ggcctggccg acgccagagg caagacggca aaaaaactgt
     3301 cccgcggcat gcgccagcgg gtgatgctgg cacgggcggt gctgcaccgg cccgagctgc
     3361 tgtttttgga cgagcccacc gcctcgctgg acccgggcac cacggccgag atccacaagc
     3421 tgctgcggca gctcaacgcc cagggcacca ctatctttct caccacccat gacatggagg
     3481 aggcggataa gctctgcggc cgggtggcct ttctgcacca cggccatatc gaggagtgcg
     3541 gcgcgcccga gcagctgaag ctcaaatacg ctgaaaacga gatcgtggcc ctgctggcgg
     3601 acggccgccg cctctcggcg gaaaagaccg tcgccggcct ggaggagctc cgccgggccg
     3661 ccggggagtg cgcgatcctc tccatccact cacaggagcc ggatctggaa gagatatttt
     3721 tgcaggtgac ggggaggaag ctggcatgaa gacacattgc acgagaacct gggtgctgct
     3781 gcgcaaggac gcggcggata tggtgaaaaa ccccaacatg ctgctcatgc tgctgctgcc
     3841 gctgctgttc gcctggatct acacctttat cggcgcggag accggcgacg ggctgccgcc
     3901 cgtcatggtg ctgaccatct ccaccctgat ggccctggcg ctggtgccgc tgagcctgct
     3961 ctccagcgct atcgcggaag aaaaagagaa gaatacgctg cgcaccctga tgctcaccgg
     4021 cgtcacggcg ggcgaatttc tcaccgccaa ggtgctggcc accgagctgg cgatggcggc
     4081 ggtgggcgca ggggtgtttt tggtcgccgg cgcgccgctg ccggtgttga tcccctatct
     4141 ggcggccatc ctgctgggcg gcttgccgct actgctgctg ggcgcggtca tcggcatcgc
     4201 cagcagggat cagatgtcca ccggcatcgc ggcggcaccg ctggcgctgc tgctgatgat
     4261 ccccaccatc ttcagcgccg tgggcggggt gatggggtgg atcgcaaagc tgacgcccac
     4321 cacggcggtg ctggaactgg tctatgccgc catgggcctg caggagcaaa gcgttgccta
     4381 cagtgtggcc gtgctggtgg tctggagcgt gctgaccgcc gcggctttcg ccctgatcta
     4441 caagaaaaag aggctggaca actgaggggg cgcccatctg ggtggacggc gccgcgaaga
     4501 ggctcccggc gcgcatccgg cgcgcatcaa atgcgcagcg cgggcaaaga ccgccggcgc
     4561 tctcattttt tatctgcacc tagggccgga taggctttgc gcagacttgc aagcggaaaa
     4621 gcggtttgca gccgcggcgg gcggcagggg ccgcaggctg cgccggggcc gggtaggggg
     4681 gctcgccctc ccgtccggtt ttccgcacca gccgggcttg gggcccgcgg cggcgcgggc
     4741 gggacacatg ggaaaggggc tgtaaaatgc tgctgtcaca aaaagagttg gggcaggctg
     4801 ccccgctctt tgccggatgg gaggagacca tgatctggtc ctgcctggag gacgcgatgg
     4861 ggcgggtgtt tgccgccggg cggccggttt gttccgcagc cgcccggatc gcggattttt
     4921 gctttctcgc cgggcggccg gacgcggcgc tgctgcggga agcggctggg cgggcggcgt
     4981 tgctcgtccc ccgggacgag gcctgggccg ggctgatcga gcgggagtgg ggcgcggggg
     5041 tgcggcggtt tgagcgctac gccacccgca aggagccgcg ggcgttcgac cggccgcggc
     5101 tgcgcggctt tgcaggtcag ctgccgcccg gctgccgcat cctgcccatc ggcgaggcgc
     5161 tctaccaccg cctggcagaa gcggattggt cgcgggattt ctgcggccag ttccccggct
     5221 ggcccgctta tcaggggcgg gggttgggct ttgcggcgct ctgtgaggga aaaccggtgt
     5281 ccggcgcctc ctcttatacc gtc
//
LOCUS       DBMDYA010000102         5300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3846_length_5300_cov_1.362377, whole genome shotgun sequence.
ACCESSION   DBMDYA010000102 DBMDYA010000000
VERSION     DBMDYA010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_5300_cov_1.362377"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="ACZPFK_03710"
     CDS             <1..194
                     /locus_tag="ACZPFK_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405453.1"
                     /translation="HYLDGLGRRPGGVAAKVACGCFLLLRNKKLISMALFLAAVRQPK
                     QMAAKTASALLRLFFIALQ"
     gene            586..1524
                     /locus_tag="ACZPFK_03715"
     CDS             586..1524
                     /locus_tag="ACZPFK_03715"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405454.1"
                     /translation="MQKLIIDADTASGDAAAIVFALRETQLQVLAVTTVAGAVPLARA
                     CRNSALAMERAGAVAVPLYAGCARPLVRPAPAQPGPWGPDGLSGAHFSEPRLAPEPEH
                     AVNALIHILRAEQEVTILALGPMTNLALALRKAPDIAGHISRVAAVAGCGLSRAAHTA
                     AAEFNVFTDPHAADIVFSSGIPLLLFPAETGLADARLPGAELAALRGCGDPAAELCLR
                     GGAWQEGYHRARYGEAFFALPEAAAAACLIHPEWIRDSLCCKVRADVSGACTLGQTIL
                     DGKAPEEERTALVATGLDRQALAACVYKTLGGEGKR"
     gene            1521..2447
                     /locus_tag="ACZPFK_03720"
     CDS             1521..2447
                     /locus_tag="ACZPFK_03720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405455.1"
                     /translation="MRKLIIDTDTASDDAIALVLALRWPDVEIKAVTTVAGNVPVEAA
                     TRNAMVSIAMADAYRPPVYRGCDRPLWRRQITAQQVHGRDGLGDRGYPDPGIPLEQEH
                     AVDAIARLVRENEGIDIVTLGPLTNIAMAIRLYPATMSRVRSITAMGGQYRQLNPHWP
                     CSEFNILVDPEAAQILLESGIHTTFVPLDVCFGEAAFDRQELDALYALGTPQARFFVD
                     CNRTLIDYTLGKYGYEELIMPDPTAMACYLEPDVALERRPAFTRCELQSTLAPGELVY
                     DFEGRCGGPENSALVPRIDAERFKRMIFDCCR"
     gene            2552..2650
                     /gene="ffs"
                     /locus_tag="ACZPFK_03725"
     ncRNA           2552..2650
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPFK_03725"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2712..4307
                     /gene="dnaX"
                     /locus_tag="ACZPFK_03730"
     CDS             2712..4307
                     /gene="dnaX"
                     /locus_tag="ACZPFK_03730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1405456.1"
                     /translation="MYHALYRKYRPKTFSDVFGQDAITAALKNQIEKGRVGHAYIFTG
                     TRGTGKTTCAKIFAKAVNCLAPHGGEPCGECDICRGLEEGSILDVVEIDAASNTGIDD
                     IRALRDEAVYTPANCKYKVYIIDEVHMLSNSAFNALLKIMEEPPSHVIFILATTEIQK
                     VPATVLSRCQRYDFGRIKQETIQKRLEMVAEKENIALSAEAAALIAQLADGALRDGLS
                     ILDTCASAEKTVDEALVRRMAGVTDKSYLFSFSEAVTAGDATRAIGLVASLRESSIDM
                     KRLCEELITHYRTVMLLGAGAGELGLSLSAEDRERYQAEAKRVPLAAAVTAMRLLGDC
                     LDRMGRGTDPRIELELALFNLCGLPAVPAAAPASKAAVQPAAPSVFAAAEKAVSAPAV
                     PQDAGAPAPSAAPQPAAPPRPAASGEGIQGEAVQPCAFWPQVIALSEQSDPMVHGFLV
                     GSSAYLQGRRVLIDGSALFLKFMKTNKDSSERLKGLIAQVTGERYSIGPYQKQTVAAE
                     AAPSRAEETFQQLQDAGVPVEYR"
     gene            4381..4728
                     /locus_tag="ACZPFK_03735"
     CDS             4381..4728
                     /locus_tag="ACZPFK_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1405457.1"
                     /translation="MKARLPQGYGKVDRNALMQQYQQMQQDMEALTAELEAREHTVTA
                     GGGQISLTMNGKYEVQRVEIKPEAVDPEDVELLEDLLAAAVNEAVRVVKETQEGEMNT
                     LTSGFNLPAGLSL"
     gene            4754..>5300
                     /gene="recR"
                     /locus_tag="ACZPFK_03740"
     CDS             4754..>5300
                     /gene="recR"
                     /locus_tag="ACZPFK_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1405458.1"
                     /translation="MGANAAPLEKLIEEFSKLPGIGRKGASRLAYQVLAMDKAEALEF
                     AQAITDAHTKIRRCSVCQNFTDGEVCPICAGEKRDKSTICVVESPRDVSAFERTREYK
                     GVYHVLHGLISPMDGVGAEQLTVKELIGRLAGDEVKEVIMATNPTVEGEATAMYLAKL
                     IKPLGVKVSRLAYGLPVGGSLE"
BASE COUNT          968 a         1631 c         1764 g          937 t
ORIGIN      
        1 cgcattatct tgacggcctt ggcaggaggc ccggcggagt agccgccaaa gtagcttgcg
       61 gctgtttttt attgcttcgc aataaaaaac ttatttctat ggccctgttt ctcgccgctg
      121 tgcggcaacc gaaacagatg gccgcaaaaa cagcctccgc tttgctgcgg ctgtttttta
      181 ttgctttgca ataaaaaaca acgtatcttg atggcctcgt ttctcgccgc tgagcggcaa
      241 ccgaaacgga tggccgcaaa aacagcctcc gctttgctgc ggctgttttt tattgcttcg
      301 taataaaaaa cttatttcta tggccctgtt tctcgccgct gagcggcaac cgaaacagat
      361 ggccgcaaaa acagcctccg ctttgctgcg gctgtttttt attgcttcgc aataaaaaac
      421 aacttatctt gatggcctcg tttctcgccg ctgcgcggca accgaaacgg atggccgaaa
      481 aaatagccca tggctatttt ttataccttt tgccggtttt caatcatctg tggatgtgat
      541 acgatggggg gcagagatca caggcgggaa aggaagctga aaacgttgca aaaactgatc
      601 attgacgcgg acaccgcatc gggagacgcg gcggccatcg tctttgcgct gcgggaaacg
      661 cagctgcagg tgttggcggt caccacagtg gcgggggcgg tgccgctggc ccgggcctgc
      721 cgcaacagcg ccctggcgat ggaacgggct ggggccgtgg ccgtgcccct ttatgcgggc
      781 tgcgcgcgcc cgctggtgcg cccggcgccg gcgcagcccg gcccctgggg accggacggg
      841 ctgtccgggg cgcatttttc agagccgcgc ctcgcgccgg agccggaaca cgcggtgaac
      901 gcgctgatcc acattctgcg tgcagagcag gaggtcacga tcctggcact ggggccgatg
      961 actaacctgg cgctggcgct gcgcaaggcg ccggatattg ccgggcatat cagccgggtg
     1021 gcggcggtgg caggctgcgg gctctcccgc gccgcgcaca ctgcggcggc ggaattcaac
     1081 gtcttcaccg atccgcacgc cgccgacatc gtcttcagct cggggatacc gctgctgctc
     1141 ttcccggccg agaccggcct tgcggacgcg cggctgccgg gggcggagct ggcggcgctg
     1201 cgcggctgcg gcgacccggc cgcagagctg tgcctgcggg gcggcgcctg gcaggagggc
     1261 tatcaccgcg cccggtacgg cgaggcgttt ttcgccctgc cggaggccgc cgcggccgcc
     1321 tgccttatcc acccggagtg gatacgcgat tctctgtgct gcaaggtgcg ggccgacgtt
     1381 tccggggcct gcactctggg gcagacgatc ctggacggca aggcgccgga ggaggagcgg
     1441 accgcactgg tcgccaccgg gctggatcgg caggcgttgg ccgcctgtgt ttataaaacg
     1501 ttgggcgggg agggaaagag atgagaaaac tgatcatcga caccgacacc gcgtcggacg
     1561 acgcgattgc actggtgctg gccctgcgct ggccggacgt ggagataaag gccgtcacca
     1621 cggtggcggg caacgtgccg gtggaggccg ccacccgcaa cgccatggtc tcaatcgcaa
     1681 tggcggacgc ctaccgcccg ccggtctacc ggggctgtga ccggccgctg tggcggcggc
     1741 agatcacggc tcagcaggtc catggccggg acggcctggg ggacaggggc tacccggacc
     1801 ccggcatccc tctggaacag gagcacgcgg tggacgccat cgcccgcctg gtacgggaaa
     1861 atgagggcat cgacatcgtc acgctgggcc cgctcaccaa tatcgccatg gccatccgct
     1921 tgtacccggc gacgatgagc cgggtgcgct ctatcaccgc catgggcggc cagtaccggc
     1981 agctcaaccc ccattggccc tgcagcgagt tcaatatcct ggtggacccg gaggcggcgc
     2041 agattttgct cgaatcgggc atccacacca cctttgtgcc cttggacgtc tgctttggcg
     2101 aggcggcctt tgaccggcag gagctggacg ccctctacgc gctcggcacg ccgcaggccc
     2161 gcttttttgt ggactgcaac cgcacgctca tcgactatac tttggggaaa tatggatatg
     2221 aagagctgat tatgcccgac cccacggcca tggcctgcta tctcgagccg gacgtggcgc
     2281 tggaacggcg gcccgctttt acccgctgcg agctgcagag cacgctggcg ccgggagagc
     2341 tggtctacga ctttgagggc cggtgcggcg ggccggaaaa cagcgccctg gtgccgcgca
     2401 tcgacgcgga gcgcttcaag cggatgatct tcgactgctg ccgctgaaag gcgggattgg
     2461 gagcgatttc ccccttgcgc tttgcgctgt tttcggctat aattattctg ttaatggaca
     2521 cggggcggcg gccgcgtgcc ccggatacga agcgttggcc ctgtgcgatg aagggccatg
     2581 aaccatgtca ggcggggaac cgagcagcat taagtggttt tctttgtgcg ccgcagcaag
     2641 cctgcgcttc gtatcggggg catgaggccc tttttccggg tcctgttttt tcgtggaggg
     2701 gggcgtctgc catgtatcac gcgctttacc gcaaataccg gcccaagact ttcagcgatg
     2761 tgtttggcca ggacgccatc accgcggccc tgaaaaacca gatcgaaaag ggtcgggtgg
     2821 ggcacgccta tatcttcacc ggtacccgcg gcaccggcaa gaccacctgc gcaaagatct
     2881 tcgccaaggc ggtcaactgt cttgcccccc acggcggcga gccctgtggg gagtgcgaca
     2941 tctgccgcgg cctggaagag ggcagtatcc tggacgtggt cgagatcgat gcggcctcca
     3001 acaccggcat cgacgacatc cgcgccctgc gggacgaggc ggtttacacg cccgcaaact
     3061 gcaaatataa ggtatatatc atcgacgagg tgcacatgct ctccaacagc gctttcaacg
     3121 cgctgctcaa gatcatggag gaaccgccct cccacgtcat cttcatcttg gccaccaccg
     3181 agatccaaaa ggtgccggcc accgtgctct cccgctgcca gaggtacgat ttcgggcgca
     3241 tcaagcagga gacgatccaa aaacggctgg aaatggtggc cgaaaaagaa aatatcgccc
     3301 tttccgccga ggccgcggcg ctgatcgccc agctggcgga cggcgcgctg cgggacggcc
     3361 tctccattct ggacacctgc gcctcggcgg agaaaacagt ggacgaggcc ctggtgcgcc
     3421 gcatggcggg cgtcaccgac aagagctatc tgttcagctt ttcagaggcg gtcacggcgg
     3481 gggacgccac ccgcgccatc gggctggtgg cgtctttgcg cgagagctcc atcgacatga
     3541 aacggctgtg tgaggagctg atcacccatt accgcaccgt catgctgctg ggcgccggtg
     3601 cgggggagct ggggctcagc ctttccgccg aggatcggga gcgctatcag gcggaggcaa
     3661 aacgggtgcc gctggcggcg gccgtcacgg cgatgcggct gctgggcgac tgtctggacc
     3721 gcatgggccg gggcaccgac ccgcgcatcg agctggagct ggcccttttc aacctgtgcg
     3781 gcctcccggc ggttccggcc gccgcaccgg cctccaaggc cgctgtgcag cccgcggctc
     3841 catcggtttt tgcagcggca gagaaagccg tgtcggcacc ggctgtgccc caggacgccg
     3901 gcgcgcctgc gccgtctgcc gcgccgcagc ccgcagcccc gccgcggccg gccgcttccg
     3961 gcgaggggat acagggagag gcggtgcagc cctgtgcatt ctggccccag gtaattgccc
     4021 tgtccgagca gagcgacccc atggtgcacg gcttccttgt gggcagtagc gcctatcttc
     4081 aggggcggcg ggtgctcatc gacggcagcg cactgttttt gaagtttatg aagaccaata
     4141 aggattcctc cgagcggctc aaggggctga tcgcccaggt caccggcgag cggtattcca
     4201 tcggccccta ccaaaagcaa acggtggccg ccgaggccgc tcccagccgc gcagaggaga
     4261 cctttcaaca attgcaggac gcgggcgtgc ccgtggaata ccgctgagcc ctgcccgctt
     4321 ttgattttat tttgagcggg cgcagacagc ggagaatata gaagctggag gaagttcaca
     4381 atgaaagcaa gattacccca ggggtatggc aaggtcgacc gcaacgcgct gatgcagcag
     4441 tatcagcaga tgcagcagga tatggaggcc ctcaccgccg agctcgaggc gcgggagcac
     4501 accgtcaccg ccggcggcgg gcagatcagc ttgaccatga acggcaagta tgaggtgcag
     4561 agggtggaga tcaagccgga ggctgtcgac cccgaggacg tggagctgct ggaggacctg
     4621 ctggccgcgg ccgtcaacga ggcggtgcgg gtggtcaagg agactcagga gggcgagatg
     4681 aacaccctga ccagcggctt caacctgccc gcgggcttga gcctgtaatt ttgatgacga
     4741 aggatgttgc ctgatgggag caaacgcggc cccgctggaa aaattgatag aggagttctc
     4801 gaagctgccc ggcatcggcc gcaagggggc ctcgcggctg gcgtatcagg tgctggccat
     4861 ggacaaggcc gaggcgctgg agtttgcgca ggcgatcacc gacgcgcaca ccaagatccg
     4921 ccgctgcagc gtctgccaga actttaccga cggtgaggtc tgcccgatct gcgccggtga
     4981 aaagcgggat aaaagcacca tctgcgtggt ggagagcccg cgcgacgtct cggcctttga
     5041 gcgcacccgc gagtacaagg gcgtttacca cgtgctgcac ggcctcatct cccccatgga
     5101 cggggtgggg gccgagcagc tcaccgtcaa agagctgatc ggccgccttg cgggcgacga
     5161 ggtgaaagag gtcatcatgg ccactaaccc caccgtggag ggggaggcca ccgccatgta
     5221 tctggcaaag ctcatcaagc cgctgggcgt caaggtcagc cgcctggcct acggcctgcc
     5281 ggtgggcggc agcctggagt
//
LOCUS       DBMDYA010000103         5279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3864_length_5279_cov_1.529741, whole genome shotgun sequence.
ACCESSION   DBMDYA010000103 DBMDYA010000000
VERSION     DBMDYA010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_5279_cov_1.529741"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="ACZPFK_03745"
     CDS             <1..798
                     /locus_tag="ACZPFK_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0032543 - mitochondrial translation
                     [Evidence IEA];
                     GO_process: GO:0032790 - ribosome disassembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HIR1405459.1"
                     /translation="DDEDIDAIIKKHPKLEPMSFAGMGELHLEIIVDRLLREFKVEAN
                     VGKPQVAYRETVRRKVDQDTKYARQSGGKGQYGHVKITIEPNESGKGYEFVNAVVGGA
                     IPKEFIGPIDQGIQGAMQAGVLAGYPVVDVKVTLYDGSYHEVDSSEMAFKIAGSMAFK
                     EAMRKADPVILEPIMKVSVIVPDDYMGDVIGDLNARRGQIQGTESRSGAQQIDAFVPL
                     SSMFGYATDLRSKTQGRGQYTMEPSHYVEVPKSIAETIMAGRAKKEG"
     gene            895..2097
                     /gene="tuf"
                     /locus_tag="ACZPFK_03750"
     CDS             895..2097
                     /gene="tuf"
                     /locus_tag="ACZPFK_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR1405460.1"
                     /translation="MGKAKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAMKGDADF
                     MDYANIDKAPEERERGITINTSHVEYQTDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKVDQVDDPELLELVEMEIRDLLSS
                     YDFPGDDTPIIQGSALKALESTSTDINAPEYKCILDLMDAVDSYIPTPDRAADQPFLM
                     PVEDVFTITGRGTVATGRVERGTIKVGEEVEIVGLQDEKRKTTITGLEMFRKLLDFAQ
                     AGDNVGALLRGIQRNEIERGQVLSKPGSIHPHTKFGGQVYILKKDEGGRHTPFFNNYR
                     PQFYFRTTDVTGVITLPEGVEMCMPGDNVTIDVELITPIAIEQGLRFAIREGGRTVGS
                     GVVSKINE"
     gene            complement(2242..2466)
                     /locus_tag="ACZPFK_03755"
     CDS             complement(2242..2466)
                     /locus_tag="ACZPFK_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405461.1"
                     /translation="MRLRDIREDHDITQKALASHLNIRQNTYSQYENGQRQLPIDILV
                     QLALYFHTSTDYILGLTDTPAPYPPGRQSP"
     gene            2584..3015
                     /locus_tag="ACZPFK_03760"
     CDS             2584..3015
                     /locus_tag="ACZPFK_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001540 - amyloid-beta binding [Evidence
                     IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0097190 - apoptotic signaling pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR1405462.1"
                     /translation="MTEPEMEQNTAAAQQEEKKEAEKFCKFCGSKIAEDAVICPCCGR
                     QVEALKGSSEGSPHIVINNANSNVNSNVNQNSVAGYGARAKNKWVAFFLCLFLGMVGA
                     HKFYEGKVGMGILYLFTVGLCGIGWLIDCVSLLLKPNPYYV"
     gene            complement(3106..4107)
                     /locus_tag="ACZPFK_03765"
     CDS             complement(3106..4107)
                     /locus_tag="ACZPFK_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HIR1405463.1"
                     /translation="MNWEDLMKEYVPQDEVLDASPLPAAEQAALLKRTLQKAGIPHEG
                     PARKKKLFFPRRLAAVCAVAVLLLSVFGVSAAFLMDGRLQALWNGGDPSTVAAVQLGQ
                     SSENKGYIATLAEAAGDENLAYFLIELQGPEGEALGQDLGFAHTTVRMEDWSHMGWSC
                     EQLPDEDPTDNRTSFLLVFESSEKMTNQKLSITLEDLYRYREEGETLAAGGSWQFETA
                     VHLSGIQKTFKPGGTLEHQDGTLHLGKVTVTPVSVTVDLRRDPLPYLLGDEAPDYTFP
                     EMSVELADGTLLTEEDRLSGSAGGKDADVMLRASYRRILNPDEVVAVILDGVRFELR"
     gene            complement(4104..4319)
                     /locus_tag="ACZPFK_03770"
     CDS             complement(4104..4319)
                     /locus_tag="ACZPFK_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019886.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1405464.1"
                     /translation="MTARLSAAAVQQAVDALDEPARTIFLRRYYVCQTVPEIARAMGL
                     SPKRVENLLFRTKPKLKTLLLKGGLAP"
     gene            complement(4316..4660)
                     /locus_tag="ACZPFK_03775"
     CDS             complement(4316..4660)
                     /locus_tag="ACZPFK_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1405465.1"
                     /translation="MKKNQAAEEAALADLLKSDPQQGLAEAIQLWGGAAKALCRGQLR
                     GLPEEEVEECLADCFAALWRDIGNWQPQRGPLKGWFYGLCRHLAAEPPAALTVCRWRK
                     ICRRRATGWRSR"
BASE COUNT         1146 a         1537 c         1528 g         1068 t
ORIGIN      
        1 gacgatgagg atatcgacgc catcataaag aagcatccga agctggagcc gatgtctttt
       61 gccggcatgg gcgagctgca tctggagatc atcgtcgacc gcctgctgcg cgagttcaag
      121 gttgaggcaa acgtgggcaa gccccaggtc gcctaccgcg aaacggtccg ccgcaaggtc
      181 gaccaggaca ccaaatacgc ccgtcagtcc ggtggtaagg gccagtacgg ccacgtcaag
      241 atcaccatcg agcccaacga gtccggcaag ggctacgagt ttgtcaatgc cgtcgtcggc
      301 ggcgccatcc ccaaagagtt tatcggcccc atcgatcagg gcatccaggg ggccatgcag
      361 gccggcgtgc ttgcgggcta ccccgttgtc gacgtcaagg tcaccctgta cgacggctcg
      421 taccacgagg tcgactcctc cgagatggcg ttcaagatcg ccggttccat ggcgttcaaa
      481 gaggccatgc gcaaggctga ccccgtcatc ctggagccca tcatgaaagt ctcggtcatc
      541 gtgccggacg attatatggg cgacgtcatc ggcgacctga acgcccgtcg tgggcagatc
      601 cagggcaccg agagccgcag cggcgcacag cagatcgacg cgtttgtgcc cctgtccagc
      661 atgtttggtt atgccaccga cctgcgcagc aagacccagg gccgcggcca gtacaccatg
      721 gagcccagcc attatgtgga ggtgcccaag agcatcgccg aaaccatcat ggcgggccgc
      781 gccaagaaag agggctaaaa gctctctccg cgcgcggata ctcttgattt ttaaggcctt
      841 acttgctaaa ataaggccaa gtgaccattt tctgaattta aggaggatac tacaatgggt
      901 aaggcaaaat ttgaccgttc caagccgcat gttaacatcg gcaccatcgg ccatgtcgac
      961 cacggcaaga ccacgctgac cgctgcgatc accaaggttc tggccatgaa aggcgatgcc
     1021 gatttcatgg actatgccaa catcgataag gctcccgagg agcgcgagcg cggcatcacc
     1081 atcaacacct cccacgtcga gtatcagacc gatacccgcc actatgccca cgttgactgc
     1141 ccgggccatg ctgactacgt caagaacatg atcaccggcg ctgcgcagat ggacggcgcc
     1201 atcctggttg tctccgctgc cgacggcccc atgccccaga cccgtgagca catcctgctc
     1261 tcccgtcagg tcggcgtgcc ttatatcgtc gtcttcatga acaaggtcga ccaggttgac
     1321 gaccccgagc tgctcgagct ggtcgagatg gaaatccgcg acctgctgtc cagctacgat
     1381 ttccccggcg acgacacccc gatcatccag ggttctgcgc tgaaggcgct ggagtccacc
     1441 tccaccgata tcaacgcccc cgagtacaag tgcatcctcg acctgatgga tgccgttgac
     1501 tcctatatcc cgaccccgga ccgcgcggct gaccagccgt tcctgatgcc ggtcgaggac
     1561 gtcttcacca tcaccggccg cggcaccgtt gccaccggtc gtgtcgagcg cggcaccatc
     1621 aaagtcggcg aggaagttga gatcgtcggc ctgcaggacg agaagcgcaa gaccaccatc
     1681 accggcctcg agatgttccg caagctgctg gacttcgctc aggctggcga caacgtgggc
     1741 gctctgctgc gcggcattca gcgcaacgag atcgagcgcg gccaggttct gtccaagccg
     1801 ggcagcatcc atccgcacac caagttcggc ggccaggtct atattctgaa aaaggatgag
     1861 ggcggccgtc acaccccgtt cttcaacaac tatcgcccgc agttctactt ccgcaccacc
     1921 gacgtcaccg gcgtgatcac cctgccggag ggcgttgaga tgtgcatgcc cggcgataac
     1981 gtcaccatcg acgttgagct gatcaccccg atcgccatcg agcagggcct gcgcttcgct
     2041 atccgcgagg gcggccgcac cgttggctcc ggcgttgtct ccaagatcaa cgagtaatcg
     2101 ctgaagatcc gtataaaaaa ggacttgcct gtaagggcaa gtcctttttt tgtagttctt
     2161 tgagagcatt ggccgggatg gcggagagag gggacaaaaa aggaagcccc gccttatttg
     2221 gtgcaataag cggcaaaagc gttatgggct ttgtcggccg ggcggataag gggccggggt
     2281 atctgtgagc cccaggatgt aatccgtaga ggtatgaaaa taaagcgcca gctgaacgag
     2341 gatatcgatg ggcagctgac gctggccgtt ttcatattgc gaataggtat tttgccggat
     2401 attgagatgg ctggccagag ccttttgcgt gatgtcgtga tcttcccgaa tgtcccgcaa
     2461 ccgcactgct gcccaccgcc tctcttttct gcttgtttca gtatatccat ctcaaattga
     2521 gatattgact tatatctcaa attgagatat atttaaagaa acagattgtg gagggggata
     2581 tttatgacag agccagaaat ggaacagaac accgcagcag cacaacagga ggaaaagaaa
     2641 gaagcggaaa agttctgcaa attttgcggc agcaagattg cggaggacgc tgtgatctgt
     2701 ccctgctgcg gcaggcaggt ggaagcgctc aaaggctcat cggagggctc gccccatatc
     2761 gtcatcaata acgcaaacag caatgtgaac agcaacgtca atcaaaacag cgtcgccggt
     2821 tatggggcga gagcgaaaaa caaatgggtt gcctttttcc tgtgcctctt tttgggcatg
     2881 gtgggcgccc acaaattcta tgagggaaaa gtggggatgg gcatccttta cttgtttacc
     2941 gtgggattat gtggcatcgg atggctgata gactgtgtga gtttgctctt gaagccgaat
     3001 ccttattatg tgtgacgaca gaaaaattat gtgtgacgag agaaaaaagc gccccctgag
     3061 agataaagcc tctcaaaggg cgctttttct gttgtcaagg gaaagttatc tcagctcgaa
     3121 ccggaccccg tccaaaatca cggccacaac ttcatccggg ttcagaatgc ggcggtagct
     3181 ggcccgcagc atgacgtctg cgtcctttcc gcccgcgctg ccggagaggc ggtcctcctc
     3241 ggtgagaagc gtgccgtccg ccaactccac actcatctca gggaaagtgt agtcgggggc
     3301 ttcgtcaccc aagagatagg gcagcgggtc gcgccgcaga tcaacggtga ccgaaacggg
     3361 ggtgacggtg accttgccca ggtgcagggt tccgtcctgg tgctctaaag tgccgccggg
     3421 cttaaaggtc ttttggatgc cggagaggtg cactgccgtt tcaaactgcc agcttccgcc
     3481 cgcggcaagg gtctcgccct cttcacggta gcgatagagg tcctccaggg tgatggagag
     3541 cttttgattg gtcatttttt ccgagctttc aaaaacaagt aaaaagctgg tgcggttgtc
     3601 ggtggggtcc tcatccggca gctgctcgca gctccagccc atatggctcc aatcctccat
     3661 ccttaccgtg gtgtgggcaa accccaggtc ctgtcccagc gcttccccct ccgggccctg
     3721 cagctcaatg aggaaatagg cgagattttc gtcgccggcc gcttcggcca gcgtggcgat
     3781 gtatcccttg ttttcgctgc tctggcccag ctgcaccgcg gccaccgtgg agggatcgcc
     3841 gccgttccag agcgcttgca ggcggccgtc catcaaaaag gcggcgctga cgccgaatac
     3901 ggagagcagc agcacggcaa ccgcacagac ggcggccaac cggcggggga aaaagagttt
     3961 ctttttgcgg gcggggccct cgtgggggat accggccttt tgcagggtac gtttcaacag
     4021 tgcggcctgt tcggcggcgg ggagaggcga ggcgtccagc acctcgtcct gtggcacata
     4081 ttctttcatc aaatcctccc aattcatggg gcaaggcctc ctttcaagag aagcgttttc
     4141 agcttgggct tggtgcggaa aagcagattt tccacccgct tgggggaaag ccccatcgcc
     4201 cgggcaatct ccggcacggt ctgacagaca taataacggc gcagaaagat cgtgcgcgcc
     4261 ggttcatcca gcgcatctac ggcctgctgc actgcggcgg cactcagacg ggcggtcacc
     4321 tgctcctcca gcccgtcgcc ctcctgcggc agatcttcct ccagcggcag actgtaagcg
     4381 cggccggcgg ctcggcggcg agatggcggc aaagcccgta gaaccagcct ttcagcggcc
     4441 cgcgctgggg ctgccagttg ccgatatcgc gccagagggc cgcaaagcaa tcggcaaggc
     4501 actcctcaac ctcctcctcc ggcagcccgc gcagctgccc ccggcagagg gccttggcgg
     4561 ccccgcccca gagctggatg gcctcggcca gcccctgttg gggatcgctt tttagcaggt
     4621 cggccagcgc ggcttcttcc gcagcctggt tttttttcat ggcttccaca cctcctttca
     4681 cttttctatt cgccggaaaa gatgagatcc actcacgatt ttgttcaaat tttatttgac
     4741 tatcagaaaa aaggggcgca gggggcatct gaacccggtc cttgcgcccc tctgcagctc
     4801 tcggctgtgg acgatttttg gccgaagccg ataaaaccgg cgtccacaaa tcagagcctg
     4861 agcagggaag agaaaaaatg ccgtcgtcct cccaggggaa gtatgggact aaaaagcagc
     4921 gaaaatttct tccgaaactg aaaaagcatc ccccgaaacg gggtgatgct tttggtcgga
     4981 gcatatcata aaaaatagcc gtaggctatt ttttcggcca tccgtttcgg ttgtcgcggc
     5041 agcgacgaga aacggggcca tcaagatcag tcgtttttta ttgcggagca ataaaaaata
     5101 gccgcaggct attttttacg cgcatccggg ccggttgccc cgcaggggcg aggcacaggc
     5161 gctttgtgat cagtttttta ttgcaaagcg gagcggagca ataaaaaata gccgctctac
     5221 ggccattttt tggccctccg cttcggttgc cgcgatagcg gcgagaagcg aggctatta
//
LOCUS       DBMDYA010000104         5266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3875_length_5266_cov_1.581994, whole genome shotgun sequence.
ACCESSION   DBMDYA010000104 DBMDYA010000000
VERSION     DBMDYA010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_5266_cov_1.581994"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            487..1404
                     /locus_tag="ACZPFK_03780"
     CDS             487..1404
                     /locus_tag="ACZPFK_03780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405466.1"
                     /translation="MVGIILWVVIGLLALVLLAGSVFMFCFVILRPRPKRGQKPRPDA
                     AEHKSAPPAHGSFQEKQQGQLRTKAWLRAQTALEEVEIRSGDGLRLHGYFLPAPQPTN
                     KIVLAVHGYKCDGLKEWGLYADYYHRRGYHLLMPDNRAHGKSEGRYIGFGWLDRLDVL
                     RWTEYLCGRFGPGCEILLHGISMGSATVLMAGGEAALCPQVKGIVADCGYTSAWDEFT
                     YQMGRNFHLPPFPFLYGADLLCRWLAGYGFRDADTYVPTEMGYRLYEACTAPKRLMIA
                     RGAIHANSYLTDPAAYEAAVSAFADSLNF"
     gene            complement(1569..2447)
                     /locus_tag="ACZPFK_03785"
     CDS             complement(1569..2447)
                     /locus_tag="ACZPFK_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405467.1"
                     /translation="MRTQKNLFALFSVFLLLSCLLVAHADMGPKDQLTVRLVNPPQEP
                     YYLDLLWQPQLQDGQQLYANLSEEERAALDGELLARLAAEAPEGWAPAFSGGTGTPLW
                     GDLEGRADGDARVHVFGYVGLPRVCRLMLVTESGKTLISEPFERKVLQSSVTFDCAAG
                     ALSQPSTAAALGVSFLLTLLPTLLIEGLLLLLFRFSLRQNWLLFLLVNLSTQVALTLA
                     VHFVLLASGPFSAHLVRFPMELVILVAETVLYRRFLRGGTAARRTAYGVTANLASWLA
                     GYLALGPTYALLVRLC"
     gene            complement(2778..3554)
                     /locus_tag="ACZPFK_03790"
     CDS             complement(2778..3554)
                     /locus_tag="ACZPFK_03790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1405468.1"
                     /translation="MTTRHLTDVHAHYDEAVFDGCRRETLLRMHEKGLCAIINSGSDL
                     PSSRRSLALAEEYPFLWASVGVFPLCAAAASQGWLSEIEVMAAHPRAVAIGEIGLDYR
                     LPDTDKAAQKAVFLAQLALAQRLDKPVVIHDCLADEDVLAALEQYPVKAMIHRFFSSV
                     QHGRRFLEKGVSLGIGPAITYPDAGELIQVVREMPLSLLLLETDCPFLPPCRLQGETA
                     TSDMLEDVVQAVAQARGDVTPQQVAEIARENAARLFSIPC"
     gene            complement(4001..5083)
                     /locus_tag="ACZPFK_03795"
     CDS             complement(4001..5083)
                     /locus_tag="ACZPFK_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405469.1"
                     /translation="MDERQKLDFLLGAVSPAGFAGYFDQMLKLRSGWHSLLIKAGPGC
                     GKSTMMNKIAGHLTGRGETVELIHCSSDPDSLDGVVCNARKFSIVDATAPHSLEPKCP
                     VAVETVVSLYDCIEEETMRKHQNEVSDLFGRCGVLQERAARYITAAGSLISDSARVAF
                     AAANLPAARSFAAHLALKYLPRTGASGEGSEELRILSANTLKGPVCYRDTVAKVADTV
                     VVLQDDWGAVSRAMMKVLRDAAVEKGYDVITCYCPMAPHDKIDHLLIPSAKLAFVTSN
                     RFHPMAFEHARVIHCTRFCDNELLRAHRSRLRFNHKAAVDLFQQASKLQAEAKQNHDQ
                     LEDYYKAAADFSKIDEVTAAIIERYC"
BASE COUNT         1029 a         1592 c         1761 g          884 t
ORIGIN      
        1 attgaagccc ttgagatacc agcaaagaaa gcgttccata tcctgctgtg aattgcagga
       61 ccaatccact gtcgccataa gctaccaccc tttcataaaa ccgcgcgccc ggccgggacg
      121 caacggcgcc attttaccat aggtgcgcgg gaaaaacaaa tcgtccgccc cggccgcgtg
      181 tgccccgtcc ataaaatccg acccggccgc cgggccgtgc ggacaatctg caaccacacc
      241 taccctggtc cgtggaaaag tggcccggga tatggtatga tgaaaaaaac aaagggcttt
      301 gtgtcgcaaa tgggtgcttt cgcacccggc gccgcagaaa ataagggtta ttgcccggca
      361 gggaaaacgg cccggggcgg ctcggccgca tcccgggatt tccgttcacg ccgggatgca
      421 gcgccgctgt tttcaaagcg aggggccggg cccctcacac aagccccaag cgggaggcgc
      481 gcaggcgtgg taggtattat tttgtgggtc gtgatcggcc ttttggcact ggtgttgttg
      541 gcgggcagcg ttttcatgtt ctgtttcgtg atcctgcgcc cgcgcccgaa gcgggggcaa
      601 aagccccgtc cggatgcggc ggagcataaa agcgcgccgc cggcgcacgg ctcttttcag
      661 gaaaagcagc agggccagct gaggacaaag gcgtggctgc gggcccagac cgcgctggag
      721 gaggtggaga tccgcagcgg ggacggcctg cgcctgcacg gctattttct gccggccccc
      781 cagcccacga acaaaatcgt gctcgccgta cacggatata agtgcgacgg cctgaaagag
      841 tgggggctgt acgcggatta ctaccaccgg cgcggctacc acctattgat gcccgacaac
      901 cgcgcccacg gcaaaagcga agggcggtat attggctttg ggtggctgga ccgtctggac
      961 gtgctgcgct ggacagaata tctttgcggg cgcttcgggc cggggtgtga gatcctgctg
     1021 cacggcatct cgatgggcag cgccaccgtg ctgatggcgg gcggcgaagc cgctttgtgc
     1081 ccgcaggtaa aaggcatcgt ggccgactgc ggctatacct cggcctggga cgaattcacc
     1141 tatcagatgg ggcgcaattt ccatctgccg ccgttcccgt tcctttacgg ggccgacctg
     1201 ctctgccgct ggctggcggg ctatggcttc cgggatgcgg acacctatgt gcccactgag
     1261 atgggatacc ggctctatga ggcctgcaca gcccccaaac gcctgatgat cgcgcgcggg
     1321 gctatccatg ccaacagcta tctcaccgac ccggccgcct acgaggccgc ggtatcggcg
     1381 tttgcggaca gcctgaattt ttgagttgca cggcgcaggc ggcgcacagc tgtttgccct
     1441 ggggggagat ggaagcgggc gagcgggcct agatgtccgc ggagctgttt gcagggcttt
     1501 ttgtttaaaa tgaaacgggg ctgttctttt tgaacagccc cgttttcttc tgccgttttg
     1561 ggatatgctc agcacaggcg caccagcagc gcataggtcg gccccaaggc cagatagcct
     1621 gccagccagc tggcgaggtt ggccgtgacg ccgtaggcgg tgcgccgcgc cgcggtgcca
     1681 ccgcgcagaa aacgccggta gagcaccgtt tcagcgacca ggatgaccag ctccatcgga
     1741 aagcgcacca gatgcgcgga aaaaggcccg gaggcgagca ggacgaagtg gacggccagg
     1801 gtgagggcca cctgcgtgga gaggttcacc aacaaaaaca gaagccagtt ttgccgcagg
     1861 gagaagcgga acagcaaaag cagcaggccc tcgatcagca gtgtgggcag cagcgtcaaa
     1921 agaaaggata cccccagcgc ggcggcagtg gaaggctggc tcagggcgcc ggcggcacag
     1981 tcgaacgtga cgctgctctg cagcaccttg cgctcaaagg gctctgagat cagcgttttg
     2041 ccgctttcgg tcaccagcat gaggcggcat acccgcggca ggcccacata gccgaacaca
     2101 tgcacccgcg cgtccccgtc cgcccggccc tcgagatcgc cccacagagg cgtgccggtg
     2161 ccgccgctga acgcgggcgc ccagccctcc ggcgcctcgg cggcgagacg ggcaagcagc
     2221 tccccgtcca gcgcggcccg ttcctcctcg ctcaggttgg cgtagagctg ctggccgtcc
     2281 tgaagctgcg gctgccagag caggtccaga taatagggct cctgcggcgg gtttaccagc
     2341 cgcacggtga gctggtcctt gggccccatg tcggcgtgcg ccaccagcag gcaggacaag
     2401 agcaggaaga cggaaaaaag tgcgaaaaga tttttttggg tgcgcataag tgcctccaat
     2461 cggtgagaga aaagagaggg cgggccgcat ggggagattg ggttcaaccg ccgcgcggcc
     2521 atgccgtggg agagggaggg cacgggacgg ctgtttttgc cgatgctccg gccggaagaa
     2581 agcacgcgag ggcaagggca gcgggaaaga caaaagggga aagcctttct cagttccaac
     2641 ggcgtcaagg ggatgtggca atccgccgca aagggagcgg ggcgacagcc ggctttgccc
     2701 gtgcgggttc tctattatac ctattatata taaggggatt ctccaggaga aaaacagaga
     2761 caggcggtct gggcgcttca acaggggatg gagaacagcc gcgcggcgtt ttcgcgcgcg
     2821 atctcagcga cctgctgggg ggtgacatcg ccccgcgcct gcgccaccgc ctgcaccacg
     2881 tcctccagca tgtccgaggt cgcggtctcg ccctgcagac ggcaaggggg cagaaagggg
     2941 cagtcggtct ccagcaacag cagcgagagc ggcatctccc gcaccacctg gatcagctcg
     3001 cccgcgtccg gataggtgat ggcggggccg atgccgagcg agaccccttt ttccagaaag
     3061 cgcctgccgt gttggacgct gctgaaaaaa cggtggatca tggccttgac cgggtactgc
     3121 tccagcgcgg cgagcacgtc ctcatccgcg aggcagtcgt ggatgaccac cggcttgtcc
     3181 agccgctgcg ccagggccag ctgggcgaga aaaacggctt tctgggcggc cttgtccgtg
     3241 tccggcaggc ggtaatccag cccgatctcc ccgatggcga cggccctggg gtgcgcggcc
     3301 atgacttcga tctccgacag ccagccctgc gacgccgcag cggcgcagag ggggaatacc
     3361 cccaccgagg cccagaggaa cggatattcc tccgccaggg ccagcgagcg gcgggaggag
     3421 ggcaggtcgg agccggagtt gatgatggcg cacaggcctt tttcatgcat gcgcagcagg
     3481 gtttcgcggc ggcagccgtc aaagacggcc tcgtcgtaat gggcgtgtac atcggtcaga
     3541 tgccgtgtcg tcatgtatgt ctcctttttc cggcgggcgg cgtgctcacg gtccggccgc
     3601 gggcgggcgc tggcaagccg cgagcacgcc atggcaagcc gcggccgggc ccggggagag
     3661 ggccgccggg aggtttcttt tttccagtat aacaagcccg cggcccgtcc gcaagggcgg
     3721 ggcgcaaaaa agagcagtga tggcagggag cgatgcgctt tgcccaacag ggcaaagctg
     3781 gacggaagcg acggtcggca gaaaagttgg ctgaaaaaat cggctgtaag aaaacggagc
     3841 accgcaagcc atgcaaagac cagcagacgg cgccgacagc ggcagacggg tctctccgcc
     3901 ggaggcaggg catatgcttc cccggccggg gtgcgccaaa gcgcccgcgt ccatcccgac
     3961 aaaaagaggg gagggcgcgg gcgctcggtg aaaaaacagg tcagcagtac cgctcgatga
     4021 tcgcggcggt cacctcgtcg attttggaaa aatcggcggc cgccttgtag taatcctcca
     4081 attgatcgtg gttctgcttg gcctccgcct gcagcttgga cgcctgctga aacaggtcca
     4141 ccgccgcctt gtggttgaac cgcaggcggc tgcggtgcgc gcgcagcagc tcgttgtcgc
     4201 agaaacgggt gcagtggatg acccgcgcgt gttcaaacgc catgggatgg aagcggtttg
     4261 aggtgacgaa agccagcttt gccgagggga tcagcaggtg gtcgatcttg tcgtgcgggg
     4321 ccatggggca gtagcaggtg atcacgtcat agcccttttc cacggcggcg tcccgcagca
     4381 ctttcatcat cgcgcggctg accgcgcccc agtcgtcctg cagcaccacc acggtgtcgg
     4441 ccaccttggc gacggtgtcc cgatagcaga ccgggccctt gagggtgttg gcggaaagga
     4501 tgcgcagctc ttcactgccc tcgccggagg cgccggtgcg gggcaggtat ttcagcgcca
     4561 ggtgcgcggc aaaagagcgg gcggcgggca ggttcgccgc agcaaaagcc acccgggcgc
     4621 tgtcgctgat caggctgccc gcggcggtga tatagcgggc ggcgcgctct tgcagaacgc
     4681 cgcagcggcc gaacaggtcc gagacctcgt tttggtgctt gcgcatggtt tcctcttcga
     4741 tacagtcgta aagggaaacg accgtttcca ccgccaccgg gcacttcggc tccagcgaat
     4801 ggggcgcggt ggcgtccaca atgctgaatt tgcgggcgtt gcagacgacc ccgtccagcg
     4861 agtccgggtc ggaggagcag tggatcagct ccacggtctc gccccggccg gtcaaatggc
     4921 ccgcgatctt gttcatcatg gtcgattttc cgcatccggg gcccgccttg atgagcaggc
     4981 tgtgccagcc gctgcgtagc ttcagcatct ggtcaaagta cccggcaaac cccgcgggag
     5041 aaaccgcccc cagcaggaaa tccagctttt gccgttcatc caaacaaatc actccctttt
     5101 agaaaaacag gcaaaagttt aaaagcgcgc ccttttcatt tgcctggttg aaccatggta
     5161 ttccgcgaga gagcggcttg ttaccgccgt gcgcccgctg agaacgccca aaaagcaaaa
     5221 aagaccggcc tttccgcttt cccctgcggg gacggaaagg ccggtc
//
LOCUS       DBMDYA010000105         5252 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3884_length_5252_cov_1.433508, whole genome shotgun sequence.
ACCESSION   DBMDYA010000105 DBMDYA010000000
VERSION     DBMDYA010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_5252_cov_1.433508"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="ACZPFK_03800"
     CDS             <1..1543
                     /locus_tag="ACZPFK_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1405470.1"
                     /translation="PPVGAPDEAALQALRGRLEEARARWQRAAQEAGAAKAASAARRD
                     ELYAAALPFFRAQGLKVAPDAPGKELRAALALLAGKLGEAAKAAQSAVAAATADWEEA
                     QALAKRHKALTQQQQAVLAACEQARAALSRLRAETAGLEGRLTALESLPLADQAAAEA
                     RLSALRSEVQRREAAREAAAAALADAQRRQAADERLLAERGEELARARASLADARAGL
                     EAALAKAGFAGEEDYADALLPPAGLEALRQAVEAARSQRVSLQETLAQLAADLQGDGQ
                     EEAGPEALAARREALAQQKEEADAQARRLYSRLAANRELARQMRQALAESGEAAKRAA
                     AAKQLADTACGSLAGKAKLDFEKYVQAAYFDNVVAAAAGRFSRMSGGQYELRRRDDLS
                     DRSGKNALDLEVVDHYTGKARSVKSLSGGESFKAALCLALGLSDVIQSAAGGVEIDAL
                     FIDEGFGSLDAGSLEQAIGVLTALASSDRLVGIISHVDELRQRIENQIAVRKTPGGSR
                     IETVL"
     gene            complement(3302..4432)
                     /locus_tag="ACZPFK_03805"
     CDS             complement(3302..4432)
                     /locus_tag="ACZPFK_03805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1405471.1"
                     /translation="MKKVILIPDSFKGTMSSAEICEIMAERVHAHWPGAETVKIPVAD
                     GGEGSVDAFVAAMGGEKVPCRVTGPRGLPCDAFYGRIGDTAVVEMAAAAGLPMMGEAR
                     DALGATTFGVGELMLAAARAGARKIIMGLGGSATNDGGCGAAAACGARFFDEAGAAFV
                     PTGGSLARVRRIDLAGLSPLLDGVKIVTMCDIDNPLCGPHGAAAVFGPQKGADAAQVA
                     LLDAGLAHLAGVMARDLGADVLQLPGAGAAGGMGGGMVAFFGSALQMGIETVLETVGF
                     EAALEGADAVFTGEGRIDGQSLRGKVVVGVARAARKKGVPVIAVVGDIADGAEAAYDE
                     GVSAIFSINRVAVPYSEAKLRAKSDLRLTMDSLLRLAELWRA"
     gene            complement(4964..>5252)
                     /locus_tag="ACZPFK_03810"
     CDS             complement(4964..>5252)
                     /locus_tag="ACZPFK_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015995.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1405472.1"
                     /translation="TEIYATDGQRMANDLVLLEDDKGFFPEYNLALLVRNDLFEEYKD
                     TAPELEEVLNLLAGRISNDAIAQMSYEVDVNKREPKDVAREWLVEQGLLSA"
BASE COUNT          934 a         1805 c         1582 g          931 t
ORIGIN      
        1 cccgccagtc ggcgcgccgg acgaagccgc cctgcaggcg ctgcgcggcc ggctggagga
       61 ggcccgcgcc cgctggcagc gggcggccca ggaggcgggc gccgccaaag ccgcctcggc
      121 ggcccggcgg gacgagctct acgccgccgc cctgcccttt ttccgcgccc aggggctgaa
      181 agtcgcccct gacgcgccgg gcaaagagct gcgcgccgcc ctggccctgc tcgcgggcaa
      241 gctgggcgag gccgccaaag cggcccaatc ggccgtcgcg gccgcgacgg ccgattggga
      301 ggaggcgcag gccctggcca aacggcacaa ggccttgacc cagcagcaac aggccgtcct
      361 tgccgcctgc gagcaggccc gcgcggccct gagccgcctg cgggcggaga ccgccgggct
      421 ggagggccgc ctgacggcac tcgaaagcct gcccctggcg gaccaggccg ctgcggaggc
      481 gcgcctttcc gcgctgcgct cagaggtgca gagaagagaa gccgcgcggg aggcggcggc
      541 agccgcgctg gccgacgccc agcggcggca ggccgccgac gaacggctgc tggccgagcg
      601 cggggaagag ctggcgcggg cgcgggcgtc gctggccgac gcccgcgcag ggctggaagc
      661 cgccctcgcc aaggcgggct ttgcgggcga agaggactat gccgacgccc tgttgccccc
      721 cgccgggctg gaggccctgc ggcaggcggt ggaggccgcc cgcagccagc gcgtctcgct
      781 gcaggagacg ctggcgcagc tggcggcgga cctgcaggga gacgggcagg aggaggccgg
      841 gccggaggcg ctggctgcgc gccgggaggc cctcgcgcag caaaaggaag aggctgacgc
      901 ccaggcccgc cggctctaca gccgccttgc cgccaaccgg gagctggcgc ggcaaatgcg
      961 gcaggcgctg gccgagagcg gcgaggcggc aaagcgcgcc gccgcggcaa agcagctggc
     1021 cgacaccgcc tgcggctccc tggcgggcaa ggccaagctg gattttgaaa aatacgtgca
     1081 ggcagcctac ttcgacaatg tggtggccgc cgccgcgggc cgtttttccc gcatgtccgg
     1141 cggccagtac gagctgcgcc gccgcgacga cctgagcgac cgctcgggca aaaacgccct
     1201 ggacctggag gtcgtcgacc actacaccgg caaggcgcgc tcggtgaagt cgctgtccgg
     1261 cggcgagtcg ttcaaggcgg cgctctgtct ggccctgggc ctgtcggacg tgatccagag
     1321 cgcggccggc ggcgtggaga tcgacgcgct gttcatcgac gagggcttcg gctcgctgga
     1381 cgctggctcg ctggagcagg ccatcggggt gctgacggcg ctggcctcct ccgaccggct
     1441 ggtgggcatc atcagccacg tggacgagct gcgccagcgc attgagaacc agatcgccgt
     1501 gcgcaagacc cccggcggca gccgcatcga gacggttctc tgacggccgc ccggcaccgc
     1561 ttggaggcag tcaaaagcgc cgccggtcaa gggcgcactg aggtgagaag ccattggttt
     1621 gggagcggcg attctgacat ccagcatatt taaaaaggtt ggcaatccac agagattgcc
     1681 aacctttttt gcaccgagcc tctttcgcac ccatcggttc acacccgccc ctccacaaga
     1741 gggaaagccg cgatacggag ccggaaaaat tcacgcgggc gccctcgccg tttgccaggt
     1801 gaatttgacg acgctccctg cgcaactctc tcttttgagg caatatcttc ttgcgaaaac
     1861 acgcccttgc ccggcgtcac ttgtagggac accaaacagc gcctttcagg aatttcccga
     1921 aaggcgctgt ccggctagaa cgccctacca acatctattt tttcaatcca cagaggacga
     1981 tactgctttt ttagtattgc cctccgacgt tttaattata aagttttggt gtcaggaatt
     2041 caatggtttg taatagaggg ttctttagag cattatagaa aacaattatt cggtgaaagt
     2101 ataagcaagc tcccctataa aaaaagccga caaaaacact gcctgtttat aaaaaagtgg
     2161 atgctgcagc caatcaccgt cattccgtct aatctttttt agaatacagg aaatagatcc
     2221 ctcaccgttt tctcttgtaa ttttctgact aaaatttttt ataatttcaa tagttgtttg
     2281 ttagagggtt aaaaaggggg attgcattac aaaagaatta gtggtaaaat gtgccaaaag
     2341 gaggtgaagc atatgaaaaa gatcactttt agcgatttga tgtcctttgg ggcctttctc
     2401 ctgcagcttt tgacgctggt ctttctcatc tcccattaaa tagttttaaa tcgctttcca
     2461 gccctctctc acccatggac agcccattgt ctcccctgca aaaaaggccc caggctattt
     2521 cgggtcatcc aggcaacaac cagccccagc gagacggcgg ggatcgcggg atagctcggg
     2581 ctttctttcc ggtgcagctc ggcgcagccc cccctctccg ccgctcgcgt gacggttccc
     2641 tcgcgaaagg cgcagtaggg ccgctgcttt gggccgcact aagaaaaaaa ccttgcaaaa
     2701 agagcaaaat ccccacatcc cgcacccttt ccaagcgcgc gccccctcct tccttttctg
     2761 accaggggag gcggcgcggc aacgccttgc cacaggggta aagcgcggtg gtgcagatga
     2821 aaaaagaaga acagaagaat aaagagcagc cgcaggcgat tctttcggcc ctgtggcccc
     2881 tccttgggct acggccatag gaataagcat ttttcggcca tccgcccgct cccggccgtg
     2941 cactctaaaa tagcaagccg tggaacagaa tagtccacag ggtcattttt tcaggcatgg
     3001 ccatccccgg gtcacagcca caaaaaagtg gtcttctgcg ctgcgataaa aaaatagctg
     3061 ctgtgcggct attttttcgg ccctgtggct catccttgac tacgaccata ggaataagca
     3121 tttttcggtc ctccgtttcg gttgccgcgg cagcggcgag aaacggagcc attaagataa
     3181 tttgtttttt attgcgcagc actaaaacgg ccgcagttta tttttgagtc catgtgaccc
     3241 atcctcaagc cacggccgtt gaaataattt gtttttatta caaagtaata aaaagtcctc
     3301 ctcatgcccg ccatagctcc gccagccgca gcaggctgtc catggtcagc cgcaggtcgc
     3361 tttttgcccg cagcttcgcc tcgctgtagg gcaccgccac ccggttgatg ctgaagatcg
     3421 cggacacccc ctcatcatag gccgcctcgg ccccgtcggc gatgtcgccc accacggcga
     3481 tcaccggcac gccctttttg cgcgccgccc gggccacccc gaccaccacc ttgccgcgca
     3541 ggctctggcc gtcgatgcgc ccctcgccgg tgaacaccgc gtccgccccc tcgagggcgg
     3601 cctcaaagcc caccgtttcc agcaccgtct cgatgcccat ctgcagcgcc gagccgaaaa
     3661 aggccaccat gccgccgccc atgccgccgg ccgcgcccgc cccgggcagc tgcagaacgt
     3721 ccgcgcccag atcccgtgcc atcaccccgg ccagatgggc cagcccggcg tccagcagcg
     3781 ccacctgcgc tgcgtcggcc cccttttggg ggccaaaaac tgccgccgcg ccgtgggggc
     3841 cgcaaagcgg gttgtcgatg tcgcacatgg tcacgatctt cacgccgtcc agcagaggcg
     3901 agaggccggc aaggtcgatg cgccggacgc gcgccaagct gccgccggtg ggcacaaagg
     3961 ccgcgccggc ctcgtcaaaa aaccgcgctc cgcaggcggc cgcggcgccg cagccgccgt
     4021 cgttggtggc gctgccgccc aggcccatga tgatctttcg cgcccccgcc cgggcggcgg
     4081 ccagcatcag ctcgcccacg ccaaaggtgg tggcccccag cgcgtcccgc gcctccccca
     4141 tcatcggcag gcccgcggcg gcggccatct ccacgacggc ggtgtcgccg atcctgccgt
     4201 aaaaggcgtc gcagggcagg ccccgcggcc cggtgacccg gcagggtacc ttttcgccgc
     4261 ccatggccgc cacaaaggcg tccacgctgc cctcgccccc gtcggccacc ggtatcttca
     4321 ccgtctcggc ccccggccaa tgggcgtgca cgcgctccgc cataatctcg cagatctccg
     4381 ccgagctcat cgtccccttg aaagaatcgg ggatcaggat cacttttttc ataggcgcct
     4441 cctccggaca aggtcttctt ccgctttata ctaccgcgcc gccgcagccc gcgcaagcct
     4501 ccggccggta aaatttttct ccgccgcact ttccgccgca cttttccact tcgccgcttt
     4561 tttctcttct cttttatccc cctgccgtgc ccgccggggc gcgcgccgcc tccagcagac
     4621 cgtgcccact ctgggacgtg cgccgcttca cggcgggacg cccgcccctc actgtgccca
     4681 taggcccatc cccgccccgc ctctgggcgg gatgctttcc tgcacgcccc agctcttggc
     4741 gggatgattc tcccgcccgc tgttgcgcgc cgtcttttgg gggacgctcc ccgcgtcccg
     4801 cctgccggag gtctatctcg ccaaaaaaga ggaggtccgg catgttcaaa gcgggggccc
     4861 gcctctcttc cccgcgtccc gcccggcgcg gaggcggggg acgcaaaaac ggcgcggcct
     4921 cagccgcgcc gttccctctt tttttgtccc tgccgccgcg gcgtcaggcg ctcagcaggc
     4981 cctgctccac cagccactcg cgggccacgt ccttgggctc gcgcttgttc acgtccacct
     5041 catagctcat ctgagcgatg gcgtcgttgc tgatccggcc cgccagcagg ttgagcacct
     5101 cctccagctc gggggcggtg tccttgtact cctcaaacag gtcgttgcgc accagcagcg
     5161 ccagattgta ctcggggaaa aagcccttgt cgtcctccag cagcaccagg tcgttggcca
     5221 tgcgctggcc gtcggtggcg tagatctcgg tg
//
LOCUS       DBMDYA010000106         5245 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3891_length_5245_cov_1.431376, whole genome shotgun sequence.
ACCESSION   DBMDYA010000106 DBMDYA010000000
VERSION     DBMDYA010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3891_length_5245_cov_1.431376"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="ACZPFK_03815"
     CDS             complement(<1..605)
                     /locus_tag="ACZPFK_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405473.1"
                     /translation="MSQDFSRIITLLRKERGITQKEAAAHLGVSQALLSHYEKGIREC
                     GLDFVVRVADFYGVSCDYLLGRSADRSGLTLKVEDLPGPESAKDGIYRGSLLPTLNKK
                     LIANSLNILFDKLNGCPNKGLVGEISGYLMLAVYRMFRLLYGSNPKNAPVLFSVPREV
                     CGGYAEAEMARAAANTKAILAGQDVGLGDPVKDTGSFAMTT"
     gene            748..1386
                     /locus_tag="ACZPFK_03820"
     CDS             748..1386
                     /locus_tag="ACZPFK_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1405474.1"
                     /translation="MKTFKLYLLRHGLTEANLNGIYAGSGTDLPLSDEGVAQLDRLRE
                     DFDYPRAGLVFVSPLLRARQSADILYPGVRQIGIEDLREIHFGEFEGRTVEELQADPV
                     YHRWLDPAQQLTPPGGESGAAFAQRAGAVLEKLCEYMVRSDTPEAAVVTHGGLIMTAL
                     AMHAIPRRPPQQWACDPGCGFAVQTSAASLMRDGTVEALEIIPYGYEAYCRR"
     gene            complement(1788..2780)
                     /locus_tag="ACZPFK_03825"
     CDS             complement(1788..2780)
                     /locus_tag="ACZPFK_03825"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405475.1"
                     /translation="MELRKKVILDVDTGSDDAVALLLAMLSGKLDILGVTVTWGNRCV
                     EDCVANTLQVVELVGADIPVYKGCPAPMVRELTPGRVANNPVNRISIVENGVEYSIHP
                     AKMPIPDAVGKPQPEHACTFLIDAVKKADSKVTLIPVGPATNIGMAFRMDPSIAENIE
                     EVVFMGGSVDMGNVTAVAEANFFHDPEAVKIVLDSGVKVRIVPLNATHSAMLTLEDAD
                     ELIGLGTRAGKLAGDMVKSRAAASLRMGATDGKAEPIHDALAVAWVLDESVMLDARRQ
                     RCDIDINGGVADGQLIVDHRNENDESVNTVVAYKADKGKFFALLKEVLARGPKL"
     gene            3064..3582
                     /locus_tag="ACZPFK_03830"
     CDS             3064..3582
                     /locus_tag="ACZPFK_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1405476.1"
                     /translation="MEQGLVEIYYGDGKGKTTAAAGLCARAAGQGLRAGFFQFLKGRA
                     SGEQAALTALGVRVSAPRQSEKFVFQMSAKERAETARIQDANLAAAALAAPGLDLLVL
                     DEALTALGLGVLDRGALLRLTREKPAGLELVITGHQPDAELFELADYITRLTAERHPY
                     ERGIGARRGIEY"
     gene            3781..4863
                     /gene="serC"
                     /locus_tag="ACZPFK_03835"
     CDS             3781..4863
                     /gene="serC"
                     /locus_tag="ACZPFK_03835"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1405477.1"
                     /translation="MARVYNFSAGPSMLPESVLKKAQSELLEYGASGQSVMEMSHRSS
                     VYDAIIRDVEATLRRVLAIPENYKVGFFQGGASTQFAMVPLNLMTTGKADYIVTGNFS
                     KKAAEEGTKFGEARVVASSKDKNFTYIPDVAAIDYSKDASYLHICENNTIFGTRYVEL
                     PQVEGVPLVADMSSCILSRPTDVSRYGLIYFGVQKNVAPAGMAVAIVREDLLGHAAAN
                     TPTMLNYKTIIDADSMYNTPPCWCIYMMGLVLHYLEDEVGGLAEMERRNNEKAALLYD
                     YLDGQDFFSNPVEKRYRSVMNVTFTSPSAELDKKFCAEAEQAGLVNLKGHRLVGGMRA
                     SIYNAMPRAGVEKLVEFMREFERKNG"
     gene            4869..>5245
                     /locus_tag="ACZPFK_03840"
     CDS             4869..>5245
                     /locus_tag="ACZPFK_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR1405478.1"
                     /translation="MQEILTLNSISPKGIERLSSSLFHVADDVEAPAGILVRSQNMHE
                     MELPESLLAIARAGAGTNNIPVDQCTARGVVVFNTPGANANAVKELTIAGLILASRNL
                     APALAWVEGLKGQGDAIAKLVEKG"
BASE COUNT         1002 a         1587 c         1704 g          952 t
ORIGIN      
        1 tcggtggtca tggcgaagct gccggtgtcc ttgaccgggt cgcccaggcc cacgtcctgg
       61 ccggccagga tggccttggt gttcgccgcc gcccgcgcca tctccgcctc ggcgtagccg
      121 ccgcagacct cccgcggcac gctgaacagc accggcgcgt ttttggggtt ggagccgtag
      181 agcaggcgga acatgcggta gaccgccagc atcagataac cgctgatttc gcccaccagc
      241 cccttgttgg ggcagccgtt cagcttgtcg aacaggatgt tgagcgaatt tgcgatcagc
      301 tttttgttga gggtgggcag caggctgccc cggtagatgc cgtccttggc gctctccggg
      361 ccgggcagat cctccacctt gagggtcagg ccgctgcggt cggcgctgcg gcccagcaga
      421 taatcgcagg aaacaccgta aaaatccgcc acccgcacca caaagtcgag cccgcactcg
      481 cggatcccct tttcatagtg ggacagcagc gcctgggaga cccccagatg cgcggcggcc
      541 tctttttggg tgatgccgcg ctctttgcgc agcagcgtga tgatacggct gaaatcctgt
      601 gacatgaata caaacccctt ttcccaccgc tgcggccccc ctgcggccgc aaaaaaatca
      661 gacaacgtac tgtaaattat aaatcagaaa aaacagattg taaataagaa aaaacgtcaa
      721 aaaacgacgt taaattttgg gggaactatg aaaacgttca aattatatct tttgcgccat
      781 ggcctgaccg aggccaacct caacggcatc tatgcgggca gcggcaccga cctgcccctc
      841 tcggacgagg gggtcgccca actggatcgg ctgcgggagg atttcgacta cccgcgcgcg
      901 ggcctggttt tcgtctcgcc gctgctgcgc gcgcggcaaa gcgccgacat cctctatccg
      961 ggcgtgcgcc agatcggcat cgaggatctg cgggagatcc attttgggga gtttgagggc
     1021 agaacggtgg aggagctgca ggcggacccg gtctatcacc gctggctgga ccccgcgcaa
     1081 cagctcaccc cgccgggcgg cgagagcggg gcggcctttg cccagcgcgc gggcgcggtg
     1141 ctggaaaagc tctgcgagta catggtgcgc agcgatacgc cggaggcggc ggtggtcacc
     1201 catggggggc tcatcatgac cgccctggcg atgcatgcca tcccccgccg cccgccccag
     1261 cagtgggcct gcgaccccgg ctgcggcttt gcggtgcaga ccagcgccgc cagcctgatg
     1321 cgggacggca cggtggaggc gctggagatc atcccctatg ggtacgaggc gtactgcagg
     1381 cgctgacggg gcgccctctg tagcaccctc cccggcccgg cggtgcagca ctgcctcccc
     1441 tggcccggag aggcaaggtc gcgcgccttg agactttttt cggcccagtc acgcagtgct
     1501 ttttcgtgcg cagccggcgg cgccctcgta tcccgctttc tggggaggct gtcgggcggt
     1561 ggctggaagg cggcatgttc ggtaataaac ccgcggggcg ctgggtagca gcgcgacaca
     1621 aaggcctcat cccaaggggg tgaggccttt gtgtttgctt tttttatctt taaattcttt
     1681 aaatactgtg cggaacggcc ttattgcatc ctcctgcgca cggccccggc gacgtgctct
     1741 gccgagaggc cgtgctgctc gaacagcgcg gcccctgcgc cgcgggctca gagcttcggt
     1801 cccctggcca gcacctcctt gagcagggcg aagaacttgc ccttgtccgc cttgtaggcg
     1861 acgacggtgt tgacgctctc gtcgttttca ttgcgatggt ccacgatcag ctggccgtcg
     1921 gccacgcccc cgttgatgtc gatgtcgcat ctttgacgcc gtgcgtccag catcaccgac
     1981 tcgtccagca cccaggccac cgccagcgcg tcgtggatgg gctccgcctt gccgtcggtg
     2041 gcgcccatgc gcaggctggc cgctgcccgg gatttcacca tatcgcccgc cagcttgccc
     2101 gcccgggtgc ccaggccgat cagctcgtcg gcgtcctcca gcgtcagcat ggccgagtgg
     2161 gtggcgttca gcggcacgat gcgcaccttt acgccggaat ccagcacgat cttcaccgcc
     2221 tccgggtcgt ggaagaagtt ggcctccgcg acggcggtca cgttgcccat gtccaccgag
     2281 ccgcccatga agacgacctc ctcgatgttt tccgcaatgg aggggtccat gcggaacgcc
     2341 atgccgatgt tggtggccgg gcccaccggg atgagggtca ccttgctgtc cgcctttttg
     2401 acggcgtcga tcaaaaaagt gcaggcgtgc tccggctgcg gcttgcccac cgcgtccggg
     2461 atgggcattt tggccgggtg gatggagtac tccaccccgt tttcgacgat ggagatgcgg
     2521 ttgaccgggt tgttcgccac gcggccgggg gtcagctccc gcaccatggg cgcagggcag
     2581 cccttgtaga ccgggatgtc cgcgcccacc agctccacca cctgcagggt gttggcgacg
     2641 cagtcctcca cacagcggtt gccccaggtc acggtcacgc ccaggatatc cagcttgccc
     2701 gagagcatgg caagcagcag ggccaccgcg tcgtccgagc cggtgtccac atccaagatc
     2761 acttttttgc gaagttccat ttttatgctc ctttcggccg ttgggccata tgttacaaaa
     2821 aaataaatca aatgattaaa aaataaagct atttcatata aacaatatac catgtttttt
     2881 tcttttttga aagagtttgc cgaaaaatag tgcggcgcgc aagtggtctt gggctgacgg
     2941 acgaacagag cgcagggcgc gcggtcgagg gaaaagcgcc gcggggagat tgactttttt
     3001 gccccgcccg ggtacacttt tcgcaagagc aacggcctgc ggccgggcag gggaggcaag
     3061 gggatggaac agggcctggt ggagatttac tacggcgacg gcaagggcaa gaccaccgcg
     3121 gcggcggggc tgtgcgcccg cgcggccggg caagggctgc gggcgggctt ttttcagttt
     3181 ttaaaggggc gcgccagcgg tgagcaggcc gcgctgacgg cgctgggggt acgggtgtcc
     3241 gcgccgcggc aaagcgaaaa atttgtcttt cagatgagcg caaaggagcg ggccgagacc
     3301 gcgcggatac aagacgccaa tctggccgcg gcggcgctgg cggcccccgg gctggatctg
     3361 ctggtgctgg acgaggccct gaccgccctg gggctggggg tgctggaccg gggggcgctg
     3421 ctgcggctga cgcgggaaaa gccagccggg ctcgagctgg tgatcaccgg gcatcagccg
     3481 gacgcagagc tgttcgagct ggcggattat atcacccgtc tcaccgccga gcgccacccc
     3541 tatgagcggg gcatcggggc gcgcaggggc attgagtatt gaaaaaacat aaaattgtcc
     3601 ttgtataaaa tacatttgta gggctatcaa aaaaagcggc ggaatccccg ccgcttttct
     3661 tgcagttttg ccgtattttt cgcgccggaa attcggtaca atgaaaagta tcgggcggcg
     3721 gggcgagagc ggccgcggcc cggcgcacga aaaaacagat catttggagg gagaacgaaa
     3781 atggctagag tgtacaactt cagcgcgggc ccgtcgatgc tgcccgagag cgtgctcaaa
     3841 aaagcccaga gcgagctttt ggagtacggg gccagcggcc aatcagtgat ggagatgagc
     3901 caccgatcta gtgtgtacga cgccatcatc cgcgatgtgg aggctactct gcggcgggtg
     3961 ctcgccatcc ccgaaaacta taaggtgggc ttttttcagg ggggggcctc cacccagttc
     4021 gccatggtgc cgctgaacct gatgaccacc ggcaaagcgg actatatcgt caccggcaac
     4081 ttttcaaaaa aggccgccga ggagggcacc aagttcggcg aggcgcgggt ggtggcctcc
     4141 agcaaggaca aaaatttcac ctatatcccg gacgtggcgg ccatcgatta ttccaaggac
     4201 gccagctacc tgcacatctg cgagaacaac accatcttcg gcacccgcta tgtagagctg
     4261 ccccaggtgg agggggtgcc cctggtggcg gatatgagca gctgcatcct ctcgcgtccc
     4321 accgacgtgt cgaggtacgg gctgatttat ttcggcgtgc agaaaaacgt ggccccggcc
     4381 ggcatggctg tggccattgt gcgggaggac ctgctgggcc acgcggcggc gaacaccccc
     4441 accatgctga attacaagac catcatcgac gcggacagca tgtacaacac cccgccctgc
     4501 tggtgcatct atatgatggg cctggtgctg cactatctgg aggacgaggt gggcggcctc
     4561 gccgagatgg agcggcgcaa caatgaaaag gcggccctgc tctacgatta tctggacggc
     4621 caggattttt tcagcaatcc ggtggagaag agataccgca gcgtcatgaa cgtcaccttt
     4681 accagcccca gcgccgagct ggacaaaaag ttctgcgccg aggcggagca ggcgggcctt
     4741 gtgaacctga aagggcaccg gctggtgggc ggcatgcgcg cctcgatcta caacgccatg
     4801 ccgcgcgcgg gcgtggaaaa gctggtggag ttcatgagag aatttgaaag gaaaaacggg
     4861 tgagcaagat gcaggagatc ctgactttaa acagcatttc ccccaagggg atcgagcggc
     4921 tgtccagcag cctgttccat gtggcggacg acgtggaggc cccggcgggc atcctggtgc
     4981 gcagccagaa catgcacgag atggagctgc ccgagagcct gctggcgatc gcccgggcgg
     5041 gcgcgggcac caacaatatc cccgtcgacc agtgcaccgc ccgcggggtg gtggtcttca
     5101 acaccccggg cgccaacgcc aacgcggtga aagagctgac catcgccggg ctgatcctgg
     5161 ccagccgcaa cctggccccg gcgctggcct gggtagaggg cctcaagggc cagggagacg
     5221 ccattgccaa gctggtggaa aaggg
//
LOCUS       DBMDYA010000107         5232 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3895_length_5232_cov_1.384720, whole genome shotgun sequence.
ACCESSION   DBMDYA010000107 DBMDYA010000000
VERSION     DBMDYA010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_5232_cov_1.384720"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(123..671)
                     /locus_tag="ACZPFK_03845"
     CDS             complement(123..671)
                     /locus_tag="ACZPFK_03845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1405479.1"
                     /translation="MKDLLLVIDMQKVYEEGEPWACRGVGRVSQNILRLLGSGRAQET
                     IFTRFDAVADPVGAWADYNREFADVNADPRLAQIVDALAQAAAQYPVYTKHTYSSLPI
                     PQVAEAVARADRVVVTGVVAECCILSTVEGLIDAGAKVVYLTDAVAGQTAEFEQAVEK
                     IVASFSPIHTSVMTVDDYLAGR"
     gene            860..1414
                     /locus_tag="ACZPFK_03850"
     CDS             860..1414
                     /locus_tag="ACZPFK_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1405480.1"
                     /translation="MTKQEAWALLTEYNHEEFHQHHARTVGAVMRWYAEKLGYGDEAD
                     FWEIAGILHDLDFEMYPEQHCKKEVEIMRGRGLDERLIHAAACHGWGLCTDLAPEHEM
                     EKVLFAADELTGLIGAAALMRPSKSVQDMELKSLKKKFKDKKFAAGCSREVITRGAEL
                     LGWDLDKLLGDTLEAMKACEEACR"
     gene            complement(1938..>3063)
                     /locus_tag="ACZPFK_03855"
     CDS             complement(1938..>3063)
                     /locus_tag="ACZPFK_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HIR1405481.1"
                     /translation="VDTLDSLERGRALRGIIREKTGKEVKTILYTHGHPDHRGGAGAF
                     ADEAPEIIAFAPKSPALGRSDALQNVYAARGGRQFGYALSDAEAVSQGIGPREGVACG
                     EHRAFVPPTTVYAEDRVCREIDGVSLELARLPGETEDQMAVWLPEKKVLCCGDNYYGC
                     FPNLYAIRGGQYRDITAWLDSLDALAAYPAEYLLPGHTAAVTGRAAIRETLVNFKNAI
                     DDLLTRTLEGMDQGKSADELAAEIRLAPAYAALPYLGEYYGCAEWTVRAIYNAYLGWF
                     DGNPTHLHPLPPRQRAARTAALMGGAQAVLAAARQALGEGEYQWCLELCDLAADSCPD
                     GGEAQAAVRLKAEALQKIALQETSANGRHYYLACAKELLG"
     assembly_gap    3064..3073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3612..4895
                     /locus_tag="ACZPFK_03860"
     CDS             3612..4895
                     /locus_tag="ACZPFK_03860"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1405482.1"
                     /translation="MRQLLTGGQVFREGAFHPEALVIENGLVSGAGVSVSKFESGNSI
                     DCRGLSILPGLVDVHVHLREPGFSFKETIASGTAAAAAGGVTQVCSMPNLSPPPDSLE
                     HLKAQLEAIRRDAQVRVTPYGCITRGQKGAGELADLEALAPCVAGFSDDGRGVQAEEL
                     MEAAMRLAKRLDKPIVAHCEDNTLLKPGGCVHEGRYAAAHGHVGISSESEWRQVERDL
                     KLAARTGCRYHVCHISTKESVALIRQAKRAGVAVTCETAPHYLLLCEDDLQEDGRFKM
                     NPPLRSAADRDALLEGLADGTVDVIATDHAPHTAEEKARGLDHSLMGVVGLETAFPLL
                     YTYLVKRGVLSLEDLVERMSLAPRRIFRLPGGIEAGQEADLAVFDLDAEYEIDPAAFL
                     SMGRATPFAGWRVQGRNTLTMVKGCVVWQRNTRER"
     gene            4868..>5232
                     /locus_tag="ACZPFK_03865"
     CDS             4868..>5232
                     /locus_tag="ACZPFK_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR1405483.1"
                     /translation="MAAEYTRKIVLENGREFYGYGFGAKAEAVNEIVFNTSMVGYQEI
                     LSDPSYTGQMVVMTYPLIGNYGVTDEDFETRVPTMGGLIVREYNDIPSNFRYTKTVGE
                     VLEENGIPGIQGVVTRALTR"
BASE COUNT          867 a         1692 c         1803 g          860 t
ORIGIN      
        1 gccggtgtgc agcgcccgcc gctccagtct ctgcgctgcg gctgcgcgcg ccggcccgcc
       61 cggctctgtg ccgcggctgc gccgaaagcg ctgccggcgc gccggcgggc aaagagcctt
      121 ggtcagcggc ccgccagata gtcgtccacg gtcatgacgc tggtgtggat gggagagaag
      181 ctggcgacga tcttttccac tgcctgctca aactcggcgg tctggccggc caccgcgtcg
      241 gtgaggtaga ccaccttggc gccggcgtcg atcagcccct ccacggtgga gaggatgcag
      301 cattcggcca ccacgccggt gaccaccacg cggtcggccc gggcgacggc ctcggccacc
      361 tgagggatgg gcagggagga ataggtgtgc ttggtgtaga ccggatactg cgcggcggcc
      421 tgcgccaggg cgtccacgat ctgcgccagg cgcgggtccg cgttcacgtc tgcaaattcg
      481 cggttgtaat ccgcccaggc ccccaccggg tctgccacgg cgtcaaagcg ggtgaagatg
      541 gtctcctgcg cccgaccgga gccgagcagg cgcaggatat tctgcgatac ccgccccaca
      601 ccgcggcagg cccagggctc gccctcctcg tacactttct gcatgtcgat caccagcaac
      661 aggtctttca tgggtggtac cctccttatc ttgagtggga aagcgctcgt ctgtctctat
      721 cctaccgcag ggcgcggctt tgcgcaagcc cgggttttgc acgcgccctt gcatccggcg
      781 ggggaggcgt tataataagt aataatcaca ctgggccgcc ccgcgggggc ggaaaaaccg
      841 aaagaggagt ggacgaaaga tgacaaaaca ggaggcgtgg gccctgctca ccgaatacaa
      901 ccacgaggag ttccaccaac accatgcaag gacggtggga gccgtgatgc gctggtacgc
      961 cgaaaagctc ggctatggcg acgaggcgga tttttgggag attgcgggca ttttgcacga
     1021 cctggatttt gagatgtacc ccgagcagca ctgcaaaaaa gaggtggaga tcatgcgcgg
     1081 gcgcgggctg gacgagcggc tgatccacgc cgcggcctgc catggctggg gcctgtgcac
     1141 cgaccttgcg cccgagcacg agatggaaaa ggtgctcttt gccgccgacg agctcaccgg
     1201 cctgatcggc gcggccgccc tgatgcggcc ctcaaagtcg gtgcaggaca tggagctgaa
     1261 gagcctgaaa aagaaattca aggacaaaaa gtttgccgcg ggctgctccc gcgaggtcat
     1321 cacccggggc gcggagctgc tgggctggga cctggacaag ctgctgggcg acaccctcga
     1381 ggccatgaaa gcctgcgagg aggcctgccg gtagggcggc cgcagcgcgg cgggggctgc
     1441 ggcccaaatc gtgacccggg acgaaaaaag gcggtgcgct gcaccgcctt ttttgctgta
     1501 ccgcaataga gaaacgagcc gccctgcaag tccggggccg gtgggcggtc ggacggcaaa
     1561 aaaagcagcg gcaaagctga tgcttttctg catgggctgc ccgcccccag cctctccggc
     1621 actcacgcac tcacgaaacg ggagcaattc cgccttgccc gaggaggcaa gcggagagga
     1681 caagacaggg caggacgggg aacctttccg ggcccatgcc cgccccgccc tcttcgcccg
     1741 cggcttttcc gctctgccct gcttcagggc agatgccgcc cctcccggcg ctcctttgcg
     1801 gggaggcagc gggtgcgggc tgccggctcc acgctcggcc ggcgcggctg ctcctcagcg
     1861 ttctgcgcag cgccgctttt ttgccccggc gcgcccgccc cgcagccgat ttgtcccgcc
     1921 gtggggcgtc tttggattca gcccagcagt tccttggcgc aggccagata gtagtggcgc
     1981 ccgttggcgc tggtctcctg cagggcgatc ttttgcaggg cctcggcctt gagacggacc
     2041 gccgcctggg cctccccgcc gtcggggcag ctgtcggcgg ccaggtcgca gagctccagg
     2101 caccactggt attccccctc gccgagggcc tgccgtgcgg ccgccagcac ggcctgggcg
     2161 ccgcccatga gggcggccgt gcgcgcggcc cgctgccggg gcggcagcgg atgcagatgg
     2221 gtggggttgc cgtcaaacca gcccagatag gcgttgtaga tcgcgcgcac cgtccactcg
     2281 gcgcagccgt agtattcccc cagatagggc agggcggcgt aggcgggggc aaggcggatc
     2341 tcagccgcca gctcatccgc gctcttcccc tggtccatgc cctccagcgt gcgggtcaaa
     2401 aggtcgtcga tggcgttttt gaagttcacc agcgtctcac ggatggcggc gcggccggtg
     2461 acggccgcgg tgtgcccggg cagcaggtac tcggcggggt aagcggccag cgcgtccagg
     2521 ctgtccagcc aggccgtgat atcccggtac tgcccgcccc ggatcgcgta caggttggga
     2581 aagcagccgt agtagttgtc cccgcagcag aggacctttt tttcgggcag ccagaccgcc
     2641 atctggtcct cggtctcgcc gggcaggcgc gccagctcca gcgatacgcc gtcgatctcg
     2701 cggcagaccc tgtcctcggc atagacggtg gtgggcggca caaaggcgcg atgctcgccg
     2761 caggcgacgc cctcgcgggg gccgatgccc tgcgacaccg cctcggcgtc gctcagcgca
     2821 tagccgaact ggcggccgcc ccgcgctgcg tagacgtttt gcagggcgtc cgaccgcccc
     2881 agcgcagggg acttgggggc aaaggcaatg atctcaggcg cctcgtcggc aaacgccccg
     2941 gcgccgccgc ggtgatccgg gtggccgtgg gtgtagagga tggtcttgac ctccttgccg
     3001 gtcttttcac gaatgatccc ccgcagcgcc cgccctcttt ccagcgagtc cagcgtgtcg
     3061 accnnnnnnn nnncaggccc aacacctgcc acaccccggc ggtgacctgg gtcaccttgg
     3121 gctgataggt cctctcggta aagtcacgga gcagttggcc cccgttttgc gtcagcatga
     3181 tttttgcctc ctgtttcctg cggcctttgc gggtcgcgtt tgctctcatg cctcccattg
     3241 tagcgcatcc gcgcgcgccg cgccaccctc ttttccaaga tcgggcgggg cgcggggcaa
     3301 aaccgccccg cggccggcgg tggggagggc aagcgcagct gcccgccccc ggcatcccgc
     3361 ccggactgtg gcggggcggc ccccgagagg ggacgctgac aaaaccggtt gaaaatcagc
     3421 gcacgttttt ggtcaaaacc gaaaagctgc ccgcgccgcc ttgacttgag aattttggtt
     3481 gcgtataatg gtgaaaacgc aaagcggctg actggcgcgg agggctgagg gccccgcgcg
     3541 ctcggctgct ttttttaggg ccgcaggcgc gccgcgcctt gcgggcaggc agtacggaac
     3601 aggagggtcc tatgcggcag ttgctcaccg gcggacaggt tttccgcgaa ggcgcttttc
     3661 accccgaggc tctcgtcatt gaaaacggcc tcgtctcggg ggccggtgtt tccgtttcaa
     3721 aatttgagag cggcaattcc attgattgcc gcgggctttc cattttgcca ggcctcgttg
     3781 atgtccacgt acatcttcgg gagcctggct tttcttttaa agagaccatc gccagcggca
     3841 ccgcggcggc cgccgccggg ggggtgaccc aggtgtgcag catgcccaac ctctcgccgc
     3901 cgccggacag cctcgaacac ctaaaggccc agctggaggc gatccgccgc gacgcgcagg
     3961 tgcgggtcac cccttacggc tgcatcaccc gcggccagaa aggcgcgggc gagctggcgg
     4021 acctggaggc cctggccccc tgtgtggcgg gcttttccga cgacgggcgg ggcgtgcagg
     4081 cggaggagct gatggaggcc gccatgcggc tggcaaagag gctggacaag cccattgtcg
     4141 cccactgcga ggacaacacg ctcttaaagc ccggcggctg cgtccacgag gggcgctacg
     4201 ccgccgccca cgggcatgtg ggcatctcct ccgagagcga gtggcggcag gtcgagcggg
     4261 acctgaagct ggcggcaagg accggctgcc gctaccacgt ctgtcacatc tcgaccaagg
     4321 agagcgtggc cctgatccgg caggcaaagc gggcgggggt ggcggtgacg tgcgagaccg
     4381 cgccccacta cctgctgctc tgtgaggatg atttgcagga ggacggccgc ttcaagatga
     4441 acccgccgct gcggtcggcg gccgaccggg acgcgctgct ggagggcctt gcggacggca
     4501 ccgtcgacgt gattgccacc gaccacgccc cccacacggc ggaggaaaag gcacgcggcc
     4561 tggaccacag cctgatgggg gtggtggggc tggagaccgc ctttccgctg ctgtacacct
     4621 atctggtgaa gcgcggggtc ttgagcctcg aggacctggt ggaaaggatg agcctggcgc
     4681 cgcggcgcat cttccggctg ccgggcggca tcgaggcggg acaggaggcg gacctcgcgg
     4741 tctttgattt agacgcggaa tacgagatcg accccgcagc gttcctctcc atgggacggg
     4801 ccaccccgtt cgcgggctgg cgggtacagg ggcgcaatac actgacgatg gtgaaaggat
     4861 gtgtcgtatg gcagcggaat acacgagaaa gatagtgctg gaaaacgggc gcgagttcta
     4921 tggctacggc tttggggcca aggcggaggc ggtgaacgag atcgtcttca acacctcgat
     4981 ggtggggtat caggagatcc tctccgaccc cagctacacc gggcagatgg tggtgatgac
     5041 ctatccgctg atcggcaact acggcgtcac cgacgaggac tttgagacgc gggtgcccac
     5101 catgggcggc ttgatcgtgc gcgagtacaa cgacatcccc tccaacttcc gctacaccaa
     5161 gacggtgggc gaggtgctgg aggagaacgg catccccggc attcagggcg tcgtcacccg
     5221 ggcgctgacc cg
//
LOCUS       DBMDYA010000108         5224 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3904_length_5224_cov_1.479602, whole genome shotgun sequence.
ACCESSION   DBMDYA010000108 DBMDYA010000000
VERSION     DBMDYA010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3904_length_5224_cov_1.479602"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="ACZPFK_03870"
     CDS             <1..501
                     /locus_tag="ACZPFK_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405484.1"
                     /translation="AGKRPSTVWLVGTVLEEYNLRGAMVAGRTVKPDIAVSLDGGGNA
                     DTPDLAGAGQVRLGGGPVLGMYNFHGRGTLNGAIPHPAMVRLTEDTAKALGMPLQRSA
                     QLGVLTDLAYVQLEGRGVMSVDLGFPIRYSHSPCEVVDLTDLERLTELVQRMLDRIDR
                     DFDRSR"
     gene            544..1632
                     /locus_tag="ACZPFK_03875"
     CDS             544..1632
                     /locus_tag="ACZPFK_03875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1405485.1"
                     /translation="MEQIETYLKELSLLPGLSGHEQRVAAYMGRHFQTRGFRVKTDTL
                     GNCIAELEGTDPSAPVVMVFAHMDSLGFVVRYIEEDGFIRIERLGGIPEKVLPATEIQ
                     IQTRSGDMIPGVIGVKAHHVTPPEEKYVVDKYTKLFVDIGAASRAEVLALGIDVGAPI
                     VYKPKFQKLRGRRVLGSAFDNRSGCAALLALADLLDGHPQPSTICLVGTVQEEYNLRG
                     AMVAGRTVKPDVAIGIDGGNAADTPDLAGQAPVALGGGPILSHYNFHGRGTLNGTIPH
                     PAMVRLMEEAADSSGVVFQRRALIGGLTDMAYLQLEGTGVRGIDVGAPRRYGHGPCEV
                     MDLRDIEAMVRWVYAALTADLAHFDWSR"
     gene            1674..3212
                     /locus_tag="ACZPFK_03880"
     CDS             1674..3212
                     /locus_tag="ACZPFK_03880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1405486.1"
                     /translation="MEKRYLLGFDVGTYESKGVLAAPDGRIVGRAARGHLLKSPHPGW
                     AEHDPIGDWWSDFRAIVAQLLADSGVRAGEIAGIGISAVMAAITPVDENCNPLRNAIL
                     YGIDTRCTAQAQALTTQIGPERMNRIFGGPCTVEHFGPKILWIKENEPEVYRRAKHFT
                     IASGFLTAKLTGEYWVDRYSVKSAVPMLDPRTMEWNDEFCRLVCPRETLPRITSTTQV
                     VGYVTAAAAAETGLAAGTPVICGTTDAGAEAVSVGVVEEGDAMLMYGSTTFFARLSRE
                     ARPGTGVWMGDYVLDGLYAYTGGMATTGSLTRWLRDNTAKELLEREAQGRLNAYDALF
                     SEAEGIPPGSDGLVVLPYFQGERMPVLDPRARGAYFGLNLRHTRGHLVHAAFEGVGYG
                     VAQNFELLRSAGMPVDTVTAVGGGTKTPQWLQVVSDICNVRQQVPEVTVGASYGDALL
                     AGLGVGLIPSPSRIREMVRIRYVVEPDPGRHAAYEPYKKVYRELYQRNKDLMHLLGEV
                     SGWT"
     gene            3203..4294
                     /locus_tag="ACZPFK_03885"
     CDS             3203..4294
                     /locus_tag="ACZPFK_03885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1405487.1"
                     /translation="MDLKQTLRELCLIPALSGHEQKLAAYLKKRFEACGLRVREDALG
                     NCIALAPGAGAAQPTVMVFGHMDQLGFVVRYIEEDGFLRLERVGGIPERALPALEVEV
                     QCRDGRMIPGVIGNKAHHATPPEEKYVVEKYTSLFVDIGAHSRAQAEGLGIRVGSPVV
                     YAGRFRELLDGRVSATSLDNRVACAVLVALAERLRDAPVAVPVALVGTVQEEFNLRGA
                     MVAGRTVRPALAVGLDIALEGGSPEMRGKNPVLMGRGPVLSLYNFHGRGTLNGTIPHP
                     GMVRLFEQTAAQQGIPLQREATMGSLTDLSYLQLEGAGVKSVDIGVPCRYTHTPAEVC
                     DLGDLQQTVDLVYAALARIPQINLEREGA"
     gene            4302..5111
                     /locus_tag="ACZPFK_03890"
     CDS             4302..5111
                     /locus_tag="ACZPFK_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1405488.1"
                     /translation="MTSWIKELFGTEKPIIAMCHLQAMPGDPYYDSAKGMKEVLEKAR
                     HDLLALQRGGVDAVMFSNEFSLPYLTRVKTETVACMARIIGELMEEIAIPYGVNCLWD
                     PIASLDLAVATEAKFVREIFTGVYASDFGLWNTDCGSIVRHQREIGAEDVRLLFNIVP
                     EAARYLTDRSIESIAKTTVFNCRPDVLCVSGATAGSATDPEVLRRAKGAVGQTPVFAN
                     TGCRPDTIENLLRIADGAVVGTYFKVDGKFENLTDAARVREFMDVVHAFRG"
BASE COUNT          902 a         1588 c         1885 g          849 t
ORIGIN      
        1 gcgggaaagc ggccctcaac ggtgtggctg gtgggcacgg tgctggagga gtacaacctg
       61 cgcggcgcca tggtggcggg tcgcaccgtc aagccggaca ttgccgtcag cctggatggg
      121 ggaggcaatg cggacacgcc cgatctcgcg ggagcggggc aggtgcgtct cggcggcggc
      181 ccggtgctgg ggatgtacaa ctttcacggg cggggcaccc tgaacggcgc catcccccac
      241 ccggccatgg tgcggctgac ggaggatacc gccaaagcac tgggcatgcc gctgcagcgc
      301 agcgcccagc tgggggtttt gaccgacctc gcctatgtcc agttggaagg gcggggcgtg
      361 atgagcgtcg acctgggctt ccccatccgc tactcccaca gcccgtgcga ggtcgtggac
      421 ctgaccgatc tggaaaggct gactgagctg gtgcagcgga tgctggaccg catcgaccgc
      481 gattttgacc ggtcgcgttg agtaggcgcc gctggacacg gcgcgcaaaa caggaggaaa
      541 ccgatggaac agatcgagac ttatttgaaa gagctgtcgc tgctgccggg cctgtcgggc
      601 catgagcaga gggtcgcggc ctatatgggg cggcacttcc aaacgcgggg gttccgcgta
      661 aagaccgata ccctgggcaa ctgtatcgcc gagctggagg gcaccgaccc gtcggcgccg
      721 gtggtgatgg tgttcgcgca catggattcg ctgggctttg tggtgcggta catcgaggag
      781 gacggcttca tccgcatcga gcggctgggg ggcatcccgg aaaaggtctt gccggccact
      841 gaaattcaga tccaaacccg cagcggcgac atgataccgg gcgtgatcgg ggtcaaggcg
      901 caccacgtga ccccgccgga ggaaaagtat gtggtggaca agtacaccaa gctctttgtg
      961 gacatcggcg cggctagccg cgcggaggtg ctggccctcg gcatcgacgt gggcgcaccc
     1021 atcgtctaca agccgaaatt tcaaaagctg aggggcagac gggtgctggg cagcgccttt
     1081 gacaaccgct ccggctgcgc ggcgctgctg gccctggcgg acctgttgga cgggcatccg
     1141 cagccctcca ccatctgttt ggtgggcacg gtgcaggagg agtacaacct gcgcggcgcc
     1201 atggtggcgg gtcgcaccgt caagccggat gtcgccatcg gcatcgacgg cggcaacgct
     1261 gccgataccc cggacctggc ggggcaggcg cccgtggccc tgggtggcgg ccctatcctc
     1321 tcccactaca actttcacgg gcggggcacc ctgaacggca ccatccccca cccggccatg
     1381 gtgcggctga tggaagaggc ggcagacagc agcggcgttg tttttcagcg ccgggcgctc
     1441 atcggcggcc tgaccgacat ggcttacctg cagctggagg gcacgggggt gcggggcatc
     1501 gatgtgggcg cgccccggcg ctatggccac ggcccctgcg aggtcatgga cctgcgggat
     1561 atcgaggcga tggtgcgctg ggtctatgcg gcgctaacgg ccgatctggc gcattttgac
     1621 tggagccgtt aaaacagcgc ccccgggaga agcggaaaag aagggagcat ctcatggaaa
     1681 aacggtatct gctggggttc gatgtgggca cctatgagtc aaaaggcgtc ctcgcggccc
     1741 ccgacggccg catcgtcggc cgggcggcgc gggggcatct gctcaagtcg ccccacccgg
     1801 gctgggcgga gcatgacccc attggggact ggtggagcga cttcagggcc atcgtcgccc
     1861 agctgctggc cgacagcggc gtgcgggcgg gggagatcgc gggcatcggc atcagcgcgg
     1921 tcatggcggc catcacgccg gtggacgaaa actgtaaccc gctgcgcaac gccatcctgt
     1981 acggcatcga cacccgctgc acggcgcagg cgcaggcgct taccacacag atcggccccg
     2041 aacggatgaa ccgcatcttc ggcggcccct gtacggtgga gcattttggg cccaagatcc
     2101 tttggatcaa ggagaatgag ccggaggtct accgccgggc aaagcatttc accatcgcct
     2161 ccggcttttt gacggccaag ctcacgggcg aatactgggt ggatcgctat tcggtcaaga
     2221 gcgcggtgcc gatgctggac ccccgcacca tggagtggaa cgacgagttc tgccgcctgg
     2281 tctgccccag ggagaccttg ccccgcatca cctccaccac ccaggtggtg gggtatgtga
     2341 cggcggcggc cgcggcggag accggccttg cggcgggcac accggtcatc tgcggcacca
     2401 ccgacgccgg ggccgaggcg gtgagcgtgg gggtggtgga agagggcgac gccatgctga
     2461 tgtatggctc caccaccttt ttcgcccgcc tctcccgcga ggcccggccg ggcaccgggg
     2521 tctggatggg ggattatgtg ctggacggcc tgtacgccta taccggcggc atggccacca
     2581 ccggctcgct gacccgctgg ctgagggaca acacggcgaa ggagctgctc gaaagggagg
     2641 cgcagggccg cctgaacgcc tatgacgcgc tgttctcaga ggcggagggc atcccgccgg
     2701 gcagcgacgg cctggtggtg ctgccctatt ttcagggcga gcggatgccg gtgctggacc
     2761 cgcgggcgcg gggggcttat tttggcctga acctgcggca cacccgcggg cacctggtac
     2821 acgcggcctt tgagggggtg ggctacggcg ttgcccaaaa ttttgagctg ctgcgcagcg
     2881 cgggcatgcc ggtggacacc gtgacggcgg tggggggcgg caccaaaacg ccccagtggc
     2941 tgcaggtggt cagcgatatc tgcaacgtgc gccagcaggt gcccgaggtg acggtgggcg
     3001 cctcttacgg cgatgcgctg ctggctgggt tgggcgtcgg gctcatcccc tcgccctccc
     3061 gcatccggga gatggtgcgc atccggtatg tggtggagcc ggaccccggg cgccatgcgg
     3121 cctatgagcc gtacaagaaa gtttaccgcg aactgtatca gagaaataag gatctgatgc
     3181 atttgttggg ggaggtgagc ggatggacct gaaacagacg ctgcgggagc tctgcctgat
     3241 cccggcgctg tccgggcacg agcaaaagct ggcggcttac ctgaaaaaac gctttgaggc
     3301 ctgcgggctg cgggtgcggg aggacgcgct gggcaactgc atcgccctgg cgccgggcgc
     3361 gggggcggcg cagcccaccg tgatggtgtt cggccacatg gaccagttgg gctttgtggt
     3421 gcggtacatc gaggaggacg gctttttgcg gctggagcgg gtggggggca tccccgagcg
     3481 ggccctgccc gccctggagg tggaggtgca gtgccgggac ggccgcatga tccccggtgt
     3541 gatcggcaac aaagcgcacc acgccacccc gcccgaagaa aaatatgtcg tggaaaaata
     3601 cacctccctg tttgtggata tcggcgcgca cagccgggcg caggcggagg ggcttggcat
     3661 ccgggtgggc agcccggtgg tgtatgcggg caggttccgg gagctgttgg acggccgggt
     3721 atccgccacc tcgctggaca accgggtggc ctgcgccgtg ctggtggcgc tggcggagcg
     3781 gctgcgggac gcgccggtcg cagtaccggt ggcactggtg ggcacggtgc aggaggagtt
     3841 caacctgcgc ggcgccatgg tggcgggcag gacggtgcgc ccagccctcg cggtggggct
     3901 ggacatcgcc ctggagggcg gctcgcccga gatgcgcggc aagaacccgg tgctgatggg
     3961 gcgggggccg gtgctcagcc tgtacaattt tcacgggcgg ggcaccctca acggcaccat
     4021 cccgcacccg ggcatggtgc ggctgttcga acagaccgcg gcgcagcagg gcatccccct
     4081 gcagcgcgaa gcgacaatgg ggtcgctcac cgacctctct tatctgcagc tcgagggcgc
     4141 cggcgtcaag tcggtggaca tcggcgtgcc ctgccggtac acccataccc ccgccgaggt
     4201 ctgcgacctg ggggacctgc aacagaccgt ggacctggtt tacgcggcgc tcgcccgtat
     4261 cccgcaaatc aatctggaaa gagaaggagc ctgagacaaa tatgacaagc tggatcaagg
     4321 aactgtttgg gacggagaag ccgatcatcg cgatgtgcca tctgcaggcg atgcccggcg
     4381 atccctatta tgacagcgca aaagggatga aagaggtgct ggaaaaagcc cgccatgacc
     4441 tgttggccct gcagcggggc ggcgtggacg cggtgatgtt cagcaacgag ttcagcctgc
     4501 cctatctgac tcgggtcaaa accgagacag tcgcctgtat ggcccgcatc atcggcgagc
     4561 tgatggagga gatcgcaatc ccctacggcg tcaactgcct gtgggacccc atcgcttcgc
     4621 tggacctggc ggtggccacg gaggcaaaat ttgtgcgcga gatctttacc ggcgtttacg
     4681 ccagcgattt cggcctgtgg aataccgact gcggctctat cgtgcgccat cagcgcgaga
     4741 tcggcgcaga ggatgtgcgg ctgctgttca acatcgtgcc ggaggcggcc cgctacctga
     4801 ccgaccgcag cattgaatcc atcgccaaga cgacggtctt caactgccgc cccgacgtgc
     4861 tctgcgtctc gggcgccacc gcgggctcgg cgaccgaccc cgaggtgctg cgccgcgcca
     4921 agggggcggt ggggcagacc ccggtatttg ccaataccgg ctgccgtccg gataccatcg
     4981 aaaacctgct gcgcattgcg gacggcgccg tggtgggcac ctacttcaag gtggacggca
     5041 aatttgaaaa cctgaccgac gcggctcgtg tgcgggagtt catggatgtg gtacacgcgt
     5101 ttcgcggata gcggagcgaa tcagaaaaag aggagggcac ccaggggggc cctcctcttt
     5161 tgaatgccgt cccggtgcgg gggggcggcg tgtccgccgc tctgccgcgc cggtcagaac
     5221 accg
//
LOCUS       DBMDYA010000109         5213 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3920_length_5213_cov_1.736111, whole genome shotgun sequence.
ACCESSION   DBMDYA010000109 DBMDYA010000000
VERSION     DBMDYA010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_5213_cov_1.736111"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="ACZPFK_03895"
     CDS             complement(<1..525)
                     /locus_tag="ACZPFK_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1405489.1"
                     /translation="MGQGKRRLGRVDLQVSLLAAALVAVSVLCMYFYQYTLAHGDMLR
                     SLENKVQGIYEYVESIAEEEDFTAINRREDEAGAQYRGLKEELERVRKIAGVRYLYTA
                     KQAQDGGFVYLVDGLDSGAEDFRHAGDPIEEEIYPQMRRALAGETLLPDDILVTSWGD
                     VFVSYYPVHAADGRV"
     gene            complement(651..1145)
                     /locus_tag="ACZPFK_03900"
     CDS             complement(651..1145)
                     /locus_tag="ACZPFK_03900"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR1405490.1"
                     /translation="MSETVFHPLAPLWDGNSRVLILGTMPSPASRSAAFYYAHPQNRF
                     WPALCAALGETDPKTAQGRGEIALRRGVALWDVLASCEIVGASDASIREARPNDLAPI
                     LTGAPIAAVFTTGKKAAQLYRRLIEPGTGRPATTLPSPSPANCAVRFEALVEAYRQIL
                     PFLS"
     gene            complement(1239..1595)
                     /locus_tag="ACZPFK_03905"
     CDS             complement(1239..1595)
                     /locus_tag="ACZPFK_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405491.1"
                     /translation="MKSLGKLVTGAALLKGAQMLPAAAGAVAVFGKTMERKLKKTQQK
                     KKGEITMKKSTLVALLVFLSAVAGALAAAYFYLVKREKELDEYEQMLFSEDYDDEDGC
                     AGCEAGAAPEQNASEE"
     gene            complement(1748..2395)
                     /locus_tag="ACZPFK_03910"
     CDS             complement(1748..2395)
                     /locus_tag="ACZPFK_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405492.1"
                     /translation="MELFAQAHFWQALGSQLLLSVLTFAFAVLLGLPLGLLAYRYHAL
                     TPPLIGLVNLLQTLPAFAVFGLFVVVGRLGAVTTLLLALLYTVLPVIFGTEKGFRRIG
                     RPPIDAALGMGMDRWQTFCRILMPLASPQIMVGLRKAAVTAVGMVTLATLRGGGLGVL
                     ILDGIQQGSGLLVVAGAVPAILMALLADGGMALLQRRLVPAPLRRRERRALYIAK"
     gene            complement(2401..3531)
                     /locus_tag="ACZPFK_03915"
     CDS             complement(2401..3531)
                     /locus_tag="ACZPFK_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405493.1"
                     /translation="MIQFENVSKSVGGVAVLTGVTFEVPTGELVVLAGPSGCGKTTTL
                     KMINRLIAPTSGRVLIDGEDVARQQVLTLRRGMGYVTQHTGLFEHLTVRQNLAVVLEL
                     EHWEEAAVARRIGELAEQFSLPGGDFLDAYPYQLTHLQKQRVCIARAFAADPDVLLMD
                     DPFASLEPGERSQMQDEFVTLQALLRKTVVFITSQMEEAVKIADRLCIMYRGAVLQYD
                     KPEEILKHPREGFVREFVGRNRIFSSPEEIRAADIMLTRPVTTHPEVPMLKGMEKMRL
                     AKVDSLLVTDEEGYFLGIVRAEHVHRCDNKEEAIKSVMREARVTAAPGDTVPELLAKI
                     RRYRVNAVPVVQDDKLVGLITNSSIVTTLSQQSIDLEEVEGL"
     gene            3971..>5213
                     /locus_tag="ACZPFK_03920"
     CDS             3971..>5213
                     /locus_tag="ACZPFK_03920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1405494.1"
                     /translation="MTFKELGLTPALLRAVSELDYVTPSPIQQRAIPPVLQGRDLIGC
                     AQTGTGKTAAFAVPILQRMQTGPAPKPSKGAARPIRALVLTPTRELALQIKESFDAYG
                     KYLPMKNTVIFGGVGQNPQVAALKSGVDILVATPGRLNDLIGQGYIDLSRVEYFVLDE
                     ADRMLDMGFIHDVRKVLRQLPEKSLRQTLLFSATMPSEIEELARQILTDPVKVAVTPV
                     SSTVDAIEQRVYKVDKANKRHLLVELLKDSSLDSVLVFTRTKHGADRVVKELGRAGVG
                     AMAIHGNKSQNARQNALESFKTGKIRVLVATDIAARGIDINELSCVVNYDLPNVPETY
                     VHRIGRTGRAGHEGLAVSFCMFDELPYLKDIEKLIKKSVPEVTDHPYPMQVFEAEPKA
                     ERQPRPGRGPQQGQPRGGRDAR"
BASE COUNT          931 a         1795 c         1592 g          895 t
ORIGIN      
        1 cacccggccg tccgcggcgt gcacggggta atagctgacg aacacgtccc cccaggaggt
       61 gaccaggata tcgtcgggca gcagcgtctc cccggccagc gcccggcgca tctgcggata
      121 gatctcctcc tcaatggggt cgcccgcgtg ccgaaaatcc tccgcgccgc tgtccaggcc
      181 gtccacaagg tagacaaagc cgccgtcctg cgcctgcttt gcggtgtaca gatagcgcac
      241 gccagcgatc ttgcgcaccc gctccagctc ctctttcagc ccccggtact gcgcgcccgc
      301 ctcgtcctcc cgccggttga tcgcggtgaa atcctcttct tcggcgatag actccacata
      361 ttcatagatg ccctgcacct tgttctccag cgagcgcagc atgtccccgt gcgccagcgt
      421 atactgataa aaatacatgc agagaacgga cacggcaacc agggcagccg ccagcagcga
      481 cacctgcagg tccacccggc caagccgtct tttcccctgt cccactgtcg cgcccccctc
      541 tccgccgatc ccctttgcag gtcacagatt catctttttc agtcttatta aaccctattt
      601 cacgagaaat agcaagcaat cttttccctg tgcagcgagg gcggcggcgc tcaggacaga
      661 aacggcagga tctgccggta cgcctccacc agcgcctcaa agcgcaccgc gcagttggcg
      721 gggctggggc tgggcagcgt ggtggccggg cggccggtgc cgggctcgat cagcctgcgg
      781 tacagctgcg cggccttttt gccggtggtg aagacggcgg cgatgggggc ccccgtcagg
      841 atcggcgcca ggtcgtttgg ccgggcctct cggatgctgg cgtcgctggc gcccacgatc
      901 tcgcagctgg ccagcacatc ccacagcgcc acgccgcggc gcagggcaat ttccccgcgg
      961 ccctgcgccg ttttggggtc cgtctcgccc agcgccgcgc agagggccgg ccagaaacgg
     1021 ttttgcggat gggcgtaata gaacgccgcc gaccgcgagg ccggcgaggg catggtgccc
     1081 aaaatgagca cgcggctgtt cccgtcccaa aggggggcga ggggatggaa gacggtctcg
     1141 gacaaggcgg ttccctcctg tctctctatt tgcggcgccg cgccgcaggg caaagcaaaa
     1201 gccggggacg gctgcccgcc cccggcccgc atcttctatt attcctctga ggcgttctgc
     1261 tccggggcgg ccccggcctc acagccggcg cagccgtcct cgtcgtcata gtcctcactg
     1321 aacagcatct gctcgtattc gtccagctct ttttcgcgct tgacaagata gaagtaggct
     1381 gcggcgagcg cgcccgcaac agcggacaga aaaacgagca gcgctaccag ggtggatttt
     1441 ttcatggtga tctccccttt ctttttttgc tgggtttttt tgagctttct ctccatcgtc
     1501 tttccaaaaa cggcgacggc gccggcagct gccggcagca tctgcgcccc cttgagcagc
     1561 gcagcgccgg tgacaagttt gccgaggctt ttcatctccg gtcaccatcc cttccggttt
     1621 ttttcattat aatatgggcg gggcgctttt acaaggactt tgcccgccct ggggacagat
     1681 ttcctcgccc gccccgcttg cagttagctt ttcccccctg agggccggtt tatgcgcgcc
     1741 gggccgctca cttggcgata tacagggcgc gtcgctccct gcggcgcaat ggcgcaggca
     1801 ccaggcgccg ctgcagcagg gccatgccgc cgtccgccag cagggccatc aagatggcgg
     1861 gcaccgcccc ggccaccacc agcagaccgc tgccctgctg gatgccgtcc aggatcagca
     1921 cgcccaagcc gccgccgcgc agggtcgcca gcgtgaccat gcccacggcg gtgaccgccg
     1981 ccttgcgcag gcccaccatg atctgggggc tggccagcgg cattaaaatg cggcagaagg
     2041 tctgccagcg gtccatcccc atgccgaggg ccgcgtcgat gggcggccgc ccaatgcgcc
     2101 gaaagccctt ttcagtgccg aagatgaccg gcagcaccgt gtagagcagc gccagcagca
     2161 gcgtggtgac cgcacccagg cgccccacca ccacaaaaag gccgaacacg gcgaacgcgg
     2221 gcagggtctg cagcagattg accagcccga tcagcggcgg ggtgagggcg tgataccggt
     2281 aggccagcag ccccagcggc agccccagca gcaccgcgaa tgcaaaggtg aggaccgaga
     2341 gcagcagctg gctgcccagc gcctgccaga agtgcgcctg tgcaaaaagc tccatcgcgc
     2401 tcaaagcccc tccacctcct ccaggtcgat cgattgctgg ctgagggtgg tgacgatgct
     2461 ggaattggtg atcagcccca ccagcttgtc gtcctgcacc accgggacgg cgttgacccg
     2521 gtagcgccgg atcttggcca gcagctcggg caccgtgtcc ccgggcgccg cggtgacccg
     2581 cgcctcccgc atcacgcttt tgatcgcctc ttccttgttg tcgcagcggt gcacgtgctc
     2641 ggcccgcacg atgccgagga aatagccctc ctcatcggtc accagcagcg agtcgacctt
     2701 ggccagccgc atcttttcca tccccttgag catcggcacc tccgggtggg tggtgaccgg
     2761 ccgggtgagc atgatgtccg cggcgcggat ctcctccggc gaggaaaaaa tgcggttgcg
     2821 gcccacgaat tcccgcacaa agccctcgcg cgggtgcttg agaatctcct ccggcttgtc
     2881 gtactgcagc accgcgcccc ggtacatgat gcacagccgg tcggcgatct tcaccgcctc
     2941 ctccatctgg ctggtgataa agaccaccgt cttgcgcagc agcgcctgca gggtgacgaa
     3001 ctcgtcctgc atctggctgc gctcgccggg ctccaggctg gcaaagggat cgtccatcag
     3061 cagcacgtcg gggtcggcgg caaaggcgcg ggcgatgcag acccgctgct tttgcaggtg
     3121 ggtcagttga tagggatacg cgtcgagaaa atctcccccc ggcaggctga actgctcggc
     3181 cagctcgccg atgcggcggg cgacggcggc ctcctcccag tgttccagct ccagcaccac
     3241 agccaaattc tggcgcacgg tgaggtgctc gaacaggccg gtgtgctggg tgacatagcc
     3301 catgccccgg cgcagcgtca gcacctgctg gcgcgccacg tcctcgccgt cgatcagcac
     3361 ccggccggag gtgggcgcga tgagccggtt gatcattttc agggtggtgg tcttgccgca
     3421 gccggagggc ccagccaaca ccaccagttc gccggtgggc acctcaaagg tgacgccggt
     3481 cagcacggcg acgcctccca ccgacttgga cacattttca aactggatca aaagctgcgc
     3541 tctccttttt tggacagagc gacaccgtgc gcgcccaaag ggcccgcacc ccgcatccgt
     3601 cccggtctct tatcttatcc acagcggggc gtccccccgc aaatctcggt ctttgcaccg
     3661 gcggtctttg cctgccggca agggcggaaa gggcaaaaaa cgcgccacat cggcgcggcg
     3721 gtccccaggg cgcggcatcc gcgccgccgc ccccgcgcgc ccgttatttt gtcatcttta
     3781 ttttaccata gcccctaaaa gttttaaagc aggggccgtc aaaaaacacc tgtttgccac
     3841 acaattcaga atttgttcat ttttcgccct attttattgg acggcaggcc caaaaccgtg
     3901 tatactaata aagttagacc ctgcggcatg ccgcggggcc attatccgct ttgagaaaag
     3961 gaacgactac atgactttta aagaactggg gctcaccccc gcgctgctgc gcgccgtgag
     4021 cgagctcgac tatgtcaccc catcccccat ccagcagcgg gccatcccgc cggtgctgca
     4081 gggccgcgac ctcatcggct gcgcccagac cggcaccggc aagaccgccg cattcgccgt
     4141 gcccatcctg cagcgcatgc agaccggccc ggcgcccaag ccgtccaagg gcgcggcacg
     4201 gcccatccgg gcgctggtgc tcacccccac ccgcgagctg gcgctgcaga tcaaagagag
     4261 cttcgacgcc tacggcaaat acctgcccat gaaaaacacc gtcatcttcg gcggcgtggg
     4321 ccagaacccc caggtagccg ccctgaagag cggcgtggac atcctggtgg ccacccccgg
     4381 ccgcctcaac gacctgatcg ggcagggcta tatcgatctg tcccgggtgg aatatttcgt
     4441 actggacgag gcggaccgga tgctggacat gggcttcatc cacgacgtac gcaaggtgct
     4501 gcgccagctg cccgaaaaat cactgcggca gaccctgctt ttttcggcca ccatgccctc
     4561 cgagatcgaa gagctggcgc ggcagatcct gaccgacccg gtcaaggtcg ccgtgacccc
     4621 ggtctcctcc acggtggacg ccatcgagca gcgggtgtac aaggtggaca aggccaacaa
     4681 gcgtcatctg ctggtggagc tgttaaagga ttccagcctg gactcggtgc tggtcttcac
     4741 ccgcaccaag cacggggccg accgggtggt caaggagctg ggccgggccg gggtgggcgc
     4801 gatggcgatc cacggcaaca agagccagaa cgcccgccag aacgcgctgg agagctttaa
     4861 gaccggcaag atccgggtgc tggtggccac cgatatcgcg gcgcggggca tcgacatcaa
     4921 cgagctgtcc tgcgtggtca actacgacct gcccaacgtg cctgagacct atgtgcaccg
     4981 catcggccgc accgggcgcg ccggccacga gggcctggcg gtcagctttt gtatgttcga
     5041 cgagctgccc tacctgaaag acatcgaaaa gctgatcaaa aaaagcgtgc ccgaggtgac
     5101 cgatcacccc tatcccatgc aggtctttga ggcggagccc aaggccgagc gccaaccccg
     5161 ccctgggcgc gggccgcagc agggccagcc gcgcggcggg cgcgacgccc ggg
//
LOCUS       DBMDYA010000110         5192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3941_length_5192_cov_1.251621, whole genome shotgun sequence.
ACCESSION   DBMDYA010000110 DBMDYA010000000
VERSION     DBMDYA010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3941_length_5192_cov_1.251621"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="ACZPFK_03925"
     CDS             complement(<1..765)
                     /locus_tag="ACZPFK_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1405495.1"
                     /translation="MNQCAAYEIERLIRFGLQQGLVGKLDAVVARNTLLDLFGLPEPY
                     EGEVPDEALDTPSAILEPLLDLAGEQGLYDKDVNALRVNFEARLMGALMPRESEVCAR
                     FEELRRTEGVKAATDYFYHLCIVSNYIRTAQIAKNIQWDYPCKYGTLEVTINLTKPEK
                     DPKTIALERLQPAASYPKCMLCLENIGYAGRVNFPARQVHRVVPVTLMGEQWYLQYSP
                     YVYYNEHCIVFGERHEPMAITHDTFRRIFDFVGQFPH"
     gene            complement(948..1880)
                     /locus_tag="ACZPFK_03930"
     CDS             complement(948..1880)
                     /locus_tag="ACZPFK_03930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1405496.1"
                     /translation="MKIVVIGAGAMGTLFGGKLALAGAEVTMVDVVPEAVESMRRDGL
                     VLELLDGVHRAPVEAYLAQDVPFIADLVVLFTKSLYSRAALESARRFMDSRTVVLSLQ
                     NGLGNLERISEFVPKKRIILGTTDFPSDLVSIGHIRSLGDGHTSIMTAEGGETPFLQQ
                     VCGLFDRAGLGCAITPDIMKTIWSKVAVNATANSITAVCRLRCGVLVDTPEAGELARH
                     IVDEIVAVAAAEGTELDGEQLKANIFALMAEMRTHLTSTEQDVLAKRPTEIGSINGGV
                     VQKARALGIPVPYNETMYLLIRTIEQNYDGQVLS"
     gene            complement(2130..3113)
                     /locus_tag="ACZPFK_03935"
     CDS             complement(2130..3113)
                     /locus_tag="ACZPFK_03935"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1405497.1"
                     /translation="MRRSELLAKAMEDVRVCRQRAVTLAERNKQRAYDKVPRLLVLDN
                     LTIETGLALARLAAARAPESTLAEKRAALKAIETERAALLAENGFAADFLQPHFHCRF
                     CQDTGTTANGLCGCVEERIRALRREELLGIAPLSECRFDNFDLLRYPDIIVPPQRVSA
                     REVMRNNLDYCRQYADGFGADCPSVFMTGDAGLGKTHLALSIAGVVLARGFNVLYVSS
                     QSAFGVVEKDRFDDGGETLQAMLEAELLILDDLGTEYLTPYVASCIYRLVDTRLTARR
                     PTIYTSNIQTQKTLNARYTEKVGSRLFGDCEILRFWGDDLRLAKKLGTAER"
     gene            complement(3113..4030)
                     /locus_tag="ACZPFK_03940"
     CDS             complement(3113..4030)
                     /locus_tag="ACZPFK_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIR1405498.1"
                     /translation="MEYQLLKNNGDAVAVPSLVLRRLAAASGDQLRVALAILRDGEVR
                     PEALLAEFPALGTVDALERHLIYWAGAGLLSTAGPTAAAPKHPPEKRPPRLSTREVAA
                     AAENDPQVAVLITESQNLFGTVLNESDGNVLASLYLQDKLPVDLILTGIAHFVTKGKR
                     SVRYIAKVLLSWREEGIRTGADMERYLAVLAQREKYEQEVAGVLGVDAAGFTSGERTL
                     IARWYEEYGYGREMIEAASQRAGDKNTVRYLNAMLKKWSAKGYHSPGELVSEGPFNTH
                     EEGRTITPENDLLLRGLDYMPKFKKRGER"
     gene            complement(4212..4287)
                     /locus_tag="ACZPFK_03945"
     tRNA            complement(4212..4287)
                     /locus_tag="ACZPFK_03945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4252..4254),aa:Gly,seq:ccc)
     gene            complement(4626..>5192)
                     /locus_tag="ACZPFK_03950"
     CDS             complement(4626..>5192)
                     /locus_tag="ACZPFK_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1405499.1"
                     /translation="AAHCIYTTPSDVKLLAESGTHLLHCTEGLGRNGDFPPMKEMFAA
                     GVHVVLGTDWLTMDPWTNMRFGVALNRHHGVDYETASARGMLRRSTIEPARALGLGDQ
                     VGSLEVGKQADVILMDVTGPNLTPMFHDPVVTLVYNACRRDVSDVMVAGKFTVRDRAL
                     QTVDAKEVMKNGQKVAYDVYGRHVATLS"
BASE COUNT          947 a         1614 c         1644 g          987 t
ORIGIN      
        1 atgggggaac tggcccacaa aatcaaagat gcgacggaac gtgtcgtggg tgatggccat
       61 gggctcatgc ctttcaccga agacgatgca gtgctcgttg taatagacat agggggagta
      121 ctgtaaatac cactgttcgc ccatcagcgt gaccggcacc acccggtgca cctgccgggc
      181 cggaaaattg acccggcccg catagccgat attttccagg cagagcatac atttgggata
      241 gctggccgcg ggctgaagcc gctccaacgc gatggtcttg gggtcttttt ccggtttggt
      301 caggttgatg gtgacctcca gcgtgccgta tttgcagggg tagtcccact ggatgttttt
      361 ggcgatctgg gcggtgcgga tatagttgct gacgatgcac agatgataga aatagtcggt
      421 ggccgccttg accccctctg tgcggcgcag ctcttcaaag cgggcgcaga cctcgctctc
      481 gcgcggcatc agcgcgccca tgagccgcgc ttcaaaattc acccgcagcg cgttcacatc
      541 cttgtcgtaa agcccctgct cgccggccag gtccagcagc ggctccaaaa tcgcggacgg
      601 agtgtccagc gcctcgtcgg gcacttcgcc ctcatagggc tccggcaggc cgaacaggtc
      661 gagcagcgtg ttgcgggcga cgacggcgtc cagcttgccg accagcccct gctgcaggcc
      721 gaagcggatc aggcgctcga tctcgtaggc ggcgcattga ttcatttatg attcctcccc
      781 ttgtgtagcg aaagcttttc cgccttttat tatagtgctt tgcgggggat ggagacatac
      841 aaaaatgttg cagccgcatg aaagaaacgg ggaacaaaaa cgcctcccgg ctgctgccgg
      901 gaggcgttcg cgcagattgc acaaaaaccg cgccgtctct ttgatgatca ggataagacc
      961 tggccgtcat agttctgctc gatggtgcgg atgagcaggt acatcgtctc gttgtagggc
     1021 acgggaatgc ccagggcgcg ggctttttgc accacgccgc cgttgatgga accgatctcg
     1081 gtgggccgct ttgccaacac gtcctgttcg gtgctggtca gatgggtgcg catctcggcc
     1141 atcagggcaa aaatattggc tttcagctgt tcgccgtcca gctcagtgcc ctctgcggcc
     1201 gccacggcga cgatctcgtc cacgatgtgg cgggccagct cgccggcctc cggggtgtcc
     1261 accagcacgc cgcagcgcag ccggcagacc gccgtgatgc tgttggccgt ggcgttcacc
     1321 gccaccttgc tccagatggt tttcatgata tccggggtga tggcgcagcc cagcccggcg
     1381 cggtcaaaaa ggccgcagac ctgctgcaaa aagggcgttt cgccgccctc ggccgtcatg
     1441 atagaggtat ggccgtcccc cagcgagcgg atatggccga tcgagacaag gtcgctgggg
     1501 aaatccgtgg tgcccaggat gatgcgtttt ttcggcacga attcgctgat gcgctccagg
     1561 ttgcccagcc cattttgcag gctaagcacc accgtgcgac tgtccatgaa ccgccgcgcg
     1621 ctctccagcg ctgcgcggga atagagcgat ttggtgaaca gcaccaccag gtcggcgata
     1681 aagggcacgt cctgggccag ataggcctcc accggggcgc ggtgcacgcc gtccagcagc
     1741 tccagcacca gcccgtccct gcgcatggac tccaccgcct ccggcaccac gtccaccatg
     1801 gtcacttccg cgcccgcgag ggccagcttt ccgccgaaga gcgtgcccat cgccccggcg
     1861 ccgatcacta cgattttcaa tgaaatcccc tcctgatggg tggaaaaggc gctttcacct
     1921 tacccgcccg atttgccgcc gcgtgggcgc ggcggacaga aaaaaggtac aggagcttga
     1981 tcctgtacca cattgtaccg atattattat agcacgccgc cgaaaaaata ctacccgccg
     2041 gaaaaaagaa aacagggggc ggcacggcgg cagggaggga caggccaggg ggctttcatc
     2101 gccgcaccta ctttttgccg ccgtctcctt tatcgctccg ctgtgcccag cttttttgcc
     2161 aggcgcaggt cgtcgcccca aaagcgcagg atctcgcagt cgccgaacag ccggctgccg
     2221 accttttcgg tatagcgggc gtttagggtc ttctgcgtct ggatgttgct ggtgtagatg
     2281 gtagggcggc gggcggtcag gcgggtgtcc accagccggt agatgcagct ggctacatac
     2341 ggggtgaggt actcggtgcc cagatcgtcc aggatgagca gctccgcctc cagcatggcc
     2401 tgcagcgtct cgccgccgtc gtcaaagcgg tccttttcca caacgccgaa cgcgctttgg
     2461 ctgctgacat acagcacatt aaagccgcgg gcaagcacca cccccgcgat ggagagggcc
     2521 agatgggtct tgcctagccc ggcgtcgccg gtcataaaga cgctggggca atcggcccca
     2581 aagccgtcgg catattggcg gcagtagtcc agattgttgc gcatgacctc gcgggcgctg
     2641 acccgctggg gcggcacgat gatgtccgga tagcgcagca gatcaaaatt gtcaaagcgg
     2701 cattcggaca ggggcgcgat ccccagcagc tcctcccggc gcagggcgcg gatgcgctct
     2761 tccacgcagc cgcacaggcc gttggcggtg gtgccggtgt cctgacagaa acggcagtgg
     2821 aaatggggtt gcaaaaagtc ggcggcaaag ccgttttcag ccagcagggc ggcccgttcg
     2881 gtctcgatgg ctttcagggc ggcacgcttt tcggccaggg tgctttccgg cgcgcgcgcc
     2941 gccgccagac gggccagggc aaggccggtc tcgatggtca ggttgtccag caccagcagc
     3001 cgcggcacct tgtcgtaggc gcgctgcttg ttgcgctcgg ccagtgtgac ggcccgctgg
     3061 cggcagacgc gcacatcctc catggctttg gccagcagtt ccgaacggcg catcagcgct
     3121 cacccctttt tttgaatttg ggcatatagt ccagcccgcg cagcaggaga tcgttttccg
     3181 gggtgatggt ccggccctcc tcatgggtgt tgaaaggccc ctctgagacc agctcgcccg
     3241 ggctgtggta gccctttgca gaccattttt tcagcatcgc attcagatag cgcaccgtgt
     3301 ttttgtcccc ggcccgctgg ctggcggcct cgatcatctc gcggccgtag ccgtattcct
     3361 cataccagcg ggcgatcagg gtgcgctcgc cggaggtgaa gcccgcggcg tccacgccca
     3421 ggacgccggc cacctcctgc tcgtactttt cgcgctgggc cagcaccgcc agatagcgct
     3481 ccatatccgc gccggtgcgg atgccctcct cccgccagga aagaagcacc ttggcgatat
     3541 accgcacgct gcgcttgccc ttggtcacaa agtgggcgat cccggtgagg atcaggtcca
     3601 ccggcagctt gtcctgcagg tagagggagg cgaggacgtt gccgtcgctc tcgttgagca
     3661 cggtaccgaa caggttctga ctctcggtga tgagaacagc cacctgcggg tcgttttcgg
     3721 ccgcggccgc cacctcgcgg gtcgagaggc ggggcggacg cttttcaggc gggtgcttgg
     3781 gcgcggcggc cgtgggaccc gcggtggaga gcaggcccgc gcccgcccag tagatcaggt
     3841 gcctttccag cgcgtcgaca gtgcccaggg cgggaaactc ggccaaaagc gcctcggggc
     3901 gcacctcgcc gtcccgcagg atggcgagcg ccacccgcag ttgatccccg cttgccgccg
     3961 ccagacggcg cagcaccagg gagggcacgg cgacggcgtc gccgttgttc ttgagcagct
     4021 ggtactccat caaaagtccc tccccaaaag aaaaagatat cccccacgcg ggcgcgccgg
     4081 cccgattgca ggaggaccgg tgaaaaacag ccgggaagct tttgcggccc gcaacgggga
     4141 gcaaagcttt aaaaaaacag aaaaaatacc cacaaaacgg cctgtgaagc cactttgtgg
     4201 gcgttttgtt ttggagcggg ttacgaggct cgaactcgcc accttctgct tgggaagcag
     4261 acgctctacc agatgagcta aacccgcatc tctaagattc agagtgcctt attattttag
     4321 ctcgcccggg caaaaaagtc aacggtttat tttgcagaaa aatgccgcgt ttttttggat
     4381 gggcagcgcg gcggcggaaa aagggatgtg cttttgtcag ccgccgccca gcggcagggg
     4441 acaggaccca acagcagggg agcgcgggga cggcggctct ctttgtcaaa atcttctgcc
     4501 gggccaaaag ggcggacggc gcgcggaaga aaagcctgcg gcagcgcgcc gccttgtgtt
     4561 gtttttacag gcctaccggc ccggcacttt tctcagcgct ccgcccgctt tttgtgcggc
     4621 gatggtcagg acagggtcgc cacatggcgg ccgtacacgt cataggcaac tttctggccg
     4681 tttttcatga cctcttttgc atccacggtc tgaagtgcgc ggtcgcgcac tgtgaacttg
     4741 cccgccacca tcacatcgct gacgtcgcgg cggcaggcgt tgtagaccag ggtcaccacc
     4801 ggatcgtgga acatgggggt gaggttcggg ccggtgacat ccatcaggat gacgtcggcc
     4861 tgcttgccca cctccaggct gcccacctgg tcgcccagcc ccagggcgcg ggccggctcg
     4921 atggtggaac ggcgcaacat cccgcgggcg ctggccgtct cgtagtccac tccgtgatgg
     4981 cggttgaggg ctacgccgaa gcgcatattg gtccaggggt ccatggtcag ccagtcggtg
     5041 cccagcacga catgcacccc ggcggcgaac atctctttca tcggcggaaa atcgccgttg
     5101 cggcccagcc cctcggtgca gtgcagcaga tgagtgccgc tctccgccag cagtttcaca
     5161 tccgaggggg tggtgtagat gcagtgcgcg gc
//
LOCUS       DBMDYA010000111         5191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3942_length_5191_cov_1.527898, whole genome shotgun sequence.
ACCESSION   DBMDYA010000111 DBMDYA010000000
VERSION     DBMDYA010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_5191_cov_1.527898"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..284
                     /locus_tag="ACZPFK_03955"
     tRNA            209..284
                     /locus_tag="ACZPFK_03955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Val,seq:gac)
     gene            complement(958..1878)
                     /locus_tag="ACZPFK_03960"
     CDS             complement(958..1878)
                     /locus_tag="ACZPFK_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMG-box domain-containing protein"
                     /protein_id="HIR1405500.1"
                     /translation="MPTIDLKAYTARAMELESAVYTQKKLMGEHQDIIKNQRPPAPAR
                     PPLQAPTEPIKPPDVLEGGTGAFGVIGGLFLIACIGFIAVSRVLGSLIFIAVVFGFGR
                     VVSNFNKDQKKRRNAVEQYKRDMEEYNHKMAQYRRKYQLADEDYANVMQSYTIRVGEY
                     DEKSAQIIQKHADVLASLEKALEEFYEQNIIFPKYRNIVAITAINEYLMSGRCFELEG
                     PDGAYNLYEMELRQNVIIGQLSSIVSDLEQIRNNQFLLYQELQKANATVESVLYEVRG
                     VRENTRLTAYFARVTALIEAFPKIYIRHSF"
     gene            complement(2084..3073)
                     /locus_tag="ACZPFK_03965"
     CDS             complement(2084..3073)
                     /locus_tag="ACZPFK_03965"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1405501.1"
                     /translation="MKHILMIGTGGTIASDITDSGLTPELSTEDLLRQVPGLEKLCRV
                     DCLQVCSLDSTNMTPARWLDIAAAVRENYDRYDGFVICHDTMAYTAAALSYLIQGSPK
                     PIVLTGSQKPIHMEITDSKTNLLDSFGCACDGRIAGVQVVFGGAVIAGTRARKNYSKS
                     FSAFSSINYPVLGVTREGKLIPYLRPEAAERPRFYDALDERVALLKLIPGARARQLEL
                     LLEENAAVIIESFGVGGVPTDGGYLEAIGAAAEKGKIVVMTTQVQNEGSDLGVYKVGH
                     ALKGAPGVLEAYDMTTEAVTAKLMWALAQTREQDGVKRLFYTPVANDILCAEQ"
     gene            complement(3164..4111)
                     /locus_tag="ACZPFK_03970"
     CDS             complement(3164..4111)
                     /locus_tag="ACZPFK_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1405502.1"
                     /translation="MKRSPARGGALLCVTALIWGFGFVAQSAGLRYIGPFTMNGVRSL
                     VAGLALLPVIPLMDRLAGRRPSLWGAAAAPAQRRTLLRGGLACGLALAAASSLQQVGI
                     AYTTVGKAGFLTALYILLVPLLGIFRGRKPAGRVWAAVAVALAGMYLLCAKEGFSIGL
                     GDGLVLLSALGFAVHILVIDHFAGKTDGVRMSCIQFFVCGALCCGAMAFAEKPTAAAL
                     AAAWAPVLYAGAVSAGAGYTLQILGQRELDPTVASLILSLESVFAALGGWLLLGQSLS
                     GRELAGCALVFAAILLAQLPAKRREGGGRGPGEKEKTAV"
     gene            4278..>5191
                     /locus_tag="ACZPFK_03975"
     CDS             4278..>5191
                     /locus_tag="ACZPFK_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1405503.1"
                     /translation="MERKDLRNIAIIAHVDHGKTTLVDEMLKQSGVFRDNQVVADRVM
                     DSGDIERERGITILSKNASTEYNGVTINIVDTPGHADFGGEVERVLKMVNGVLLLVDA
                     AEGPMPQTRFVLQKALDQNLSIVIVVNKIDRPDARIKEVIDEVLLLLMDMGATDEQLD
                     SPMVFCSGRAGTASYSPDAPGTDLKPLFDTIVEYVKPPVGDPNAPLQCLVSCIDYNEF
                     VGRTSIGRIDAGTMKVGQNVTVCDWHNKDLCKNARITALYAFDGVGRKEIESASAGDI
                     VMFSGIDDVTIGNTICDAGVPNPIPFVK"
BASE COUNT         1098 a         1626 c         1397 g         1070 t
ORIGIN      
        1 cgagaatgcg cacgcgcagc cctccttttc agcatggtcc gattgcgagc ttattaaccg
       61 atttattata gcacaaagca ttctgtgggg caaccgcacc cgcttgacgc ccggcgcgca
      121 aaaaatattt ttttgaaaaa aagggttgat tttacagcgc cggcattata taatataaaa
      181 cgtcgcacga cacatggcga caggatcagg gcggttagct cagctggtag agcacctgct
      241 tgacgtgcag ggggtcaggg attcgagttc cttaccgccc accaaataaa aggcatccaa
      301 atggatgcct tttatttttt tgctttacac gcggcctttt gagagcgccg cccctttggg
      361 gccgccgctt tacctcggag gcgcggccaa ggccgggggg cggcggccgg acaaaagaaa
      421 aagaggcgcc caaagggcgc gtctttgcaa aaacccgctc ccgcgagcgg gagatctccg
      481 cgcagagtat cgtccaattc gccccgccgg gtgcagagca ttctgcggtt ttgctccgcg
      541 aaccgcccgc gcgggcaggc aatccgcgca aattttacca gcgccgcatt gcagctttcc
      601 ctcgcggagg ggtaccgggc agttttgagg aaagtatcgt acaaaaaagt atccgccagc
      661 ttggctggcg gatacttttt tgtacattga gccatgccgt cggacagcgc tcgtccgttt
      721 cgctctttga gatccaaggc ggcgatgtcc gccggttttc ctctgggaaa tggtgctttt
      781 taggagggga aacaaaagat aacaagcagc gcagatcgat accgaccatg cgttttctta
      841 ttttgcccct ccaaaccccc gaaagatttt ttcaaaatcg gctgcactca tgcagagttc
      901 ttcaaacaga tactgttgta aacaaggggt atttcttatg actacccagc taatgcctta
      961 aaaggaatgc cttatataaa ttttaggaaa agcctcaatc aaagcggtaa cacgagcaaa
     1021 ataagccgtt aatctggtgt tctcacgcac accgcggact tcatacaaaa cactttcgac
     1081 tgtggcattg gctttttgca attcttggta tagcaaaaat tgattatttc gaatctgttc
     1141 caagtcgcta acaatgctgg acaattggcc aatgataacg ttctgcctca attccatttc
     1201 gtagagatta tatgctccgt cgggaccttc aagttcaaaa cagcgaccac tcattaagta
     1261 ttcgtttatc gctgtaatgg cgactatatt tctgtacttt ggaaaaatta tattctgttc
     1321 gtaaaattct tcgagggctt tctctaaaga agccagaaca tcggcatgct tttgtataat
     1381 ttgagcgctt ttttcatcgt attcgccaac gcgaatggta tagctttgca tgacattagc
     1441 atagtcttcg tcagccagtt ggtatttgcg ccggtattgt gccatcttat gattatattc
     1501 ctccatatcc cgcttgtatt gctcaactgc gtttcttctt ttcttttggt ccttgttaaa
     1561 attggacact acacgtccaa atccaaagac aacagcaatg aatatcaaac tgcccaatac
     1621 gcgagaaacc gctataaagc caatgcatgc gataagaaat agtcctccaa taaccccaaa
     1681 ggcgccagta cctccctcga gaacgtcagg aggtttaatc ggctctgttg gggcctgcaa
     1741 aggaggtctt gccggtgccg gaggccgttg attttttata atatcctgat gctcacccat
     1801 tagttttttt tgcgtataga cggctgattc cagttccatc gctctggctg tatatgcttt
     1861 caagtcaata gtaggcatcg actgccctcc atcccgttaa tatataagtt tttcatctta
     1921 aaagcaacct atagcgtcca gccgaggctg aatgccgtag gttcgtttat ttcaatcttg
     1981 ctcgaaagat gtcaatttca caattttcat caagtcacta tcttatcggc acccgcccga
     2041 ggcgctctct tttcaatccc gggcggaaaa atctttcggg cgctcactgc tccgcacaga
     2101 ggatatcgtt tgccaccggc gtgtagaaca ggcgtttcac cccatcctgc tcccgcgtct
     2161 gtgccagcgc ccacatcagc ttggcggtga ccgcctcggt ggtcatatcg taggcctcca
     2221 gcacgcccgg cgcgcccttg agcgcgtggc ccaccttgta tacgccgaga tcgctgccct
     2281 cattctgcac ctgggtggtc atgaccacga tcttgccctt ttccgccgcc gccccgatcg
     2341 cttccagata gcccccgtcg gtgggcaccc cgcccacgcc gaagctctcg atgatgacgg
     2401 cggcgttctc ctccagcagc agctccagct gccgcgcccg cgcgccgggg atcagcttga
     2461 gcagggccac ccgctcgtcc aaagcgtcgt aaaagcgggg ccgttccgcc gcctcggggc
     2521 gcaggtaggg gatcagcttg ccctcccgcg tcacccccag caccgggtag ttgatgctcg
     2581 aaaaggcgga aaagcttttg gaataattct tgcgcgcccg cgtgcccgcg atgaccgcgc
     2641 ccccgaacac cacctgcacc ccggcgatgc gcccgtcgca ggcgcagccg aagctgtcca
     2701 gcaggttggt ctttgagtcg gtgatctcca tgtgaatggg cttttggctg ccggtgagca
     2761 cgatgggctt ggggctgccc tggatgaggt agctcagggc ggccgccgtg taggccatgg
     2821 tgtcgtgaca gatgacgaag ccgtcgtagc ggtcatagtt ctcccgcacc gcggccgcga
     2881 tgtccagcca gcgcgccggg gtcatgttgg tgctgtccag gctgcacacc tgcaggcagt
     2941 ccaccctgca cagcttttcc agccccggca cctggcgcag caggtcctcg gtggacaact
     3001 caggcgtgag gcccgagtcc gtgatatccg aggcgatggt cccgccggtg ccgatcatca
     3061 aaatgtgttt cataacgccc tcctcaaaaa aggccgcgcc gggccgcagg cactctgcgg
     3121 cggcttcttc cctgtccctc agtataccac ttcggcccgc tgattacacc gccgttttct
     3181 ctttttctcc cggcccgcgg ccgccgccct cgcgcctttt ggcgggcagc tgggccagca
     3241 ggatggccgc aaagaccagc gcgcagccag ccagctcgcg cccgctcagc gactgcccca
     3301 gcagcagcca acctcccagc gccgcaaaga ccgattccag gctgaggatc agcgaggcca
     3361 cggtggggtc cagctcccgc tggcccagga tctgcagcgt gtaccccgcg cccgcggaga
     3421 ccgcgcccgc atagagcacc ggggcccagg cggccgccag agcagcggcg gtgggctttt
     3481 ctgcaaaggc catcgccccg cagcagagcg cgccgcagac aaagaactgg atgcaggaca
     3541 tccgcacccc gtccgttttg cccgcaaagt ggtcgatgac caggatatgc accgcaaagc
     3601 cgagggcgct gagcagcacc aggccgtcgc ccagcccgat ggaaaagccc tcctttgcgc
     3661 acagcaggta catgcccgcc agcgccacgg ccacggccgc ccagacccgg cccgccggct
     3721 ttcgcccgcg aaagatgccc agcagcggca ccagcaggat ataaagcgcc gtcagaaagc
     3781 ccgccttgcc caccgtggta taggcgatgc ccacctgctg cagcgaggac gccgccgcca
     3841 gcgccaggcc gcaggcgagg ccgccgcgca gcagggtccg tcgctgggct ggcgcggcgg
     3901 ccgcacccca cagcgagggc cgccggcccg ccagccggtc catcaggggg atcaccggca
     3961 gcagcgccag cccggcgacg aggctgcgca ccccgttcat cgtgaaaggg ccgatgtacc
     4021 gcagccccgc cgactgggcc acaaagccaa agccccagat cagcgcggtc acacacaaca
     4081 gcgcgccgcc gcgcgcgggc gatctcttca ctccgcttcc ctcccctttg acacaacttc
     4141 ctcattttac catgcgcgcc gccaaaaatc cacctccgcc gaccttttcc cgccgactgc
     4201 gcgccttgac atttgctctg ttatattata aaatattacg tctgcatata ggaatttgag
     4261 aagaaggaac gggaagtatg gagagaaaag atctgcgcaa tatcgcgatc atcgcccacg
     4321 ttgaccacgg caaaacgacc ctggttgacg agatgctgaa acagagcggc gtcttccgtg
     4381 acaaccaggt ggtcgccgac cgcgtgatgg actcgggcga catcgaacgg gaacgcggca
     4441 tcaccatttt atcgaaaaac gcctccaccg agtacaacgg cgtcaccatc aatatcgtgg
     4501 acaccccggg acacgccgat ttcggcggcg aggtcgagcg ggtgctgaag atggtcaacg
     4561 gcgtgctgct gctggtggac gccgcggagg ggccgatgcc ccagacccgc tttgtgctgc
     4621 aaaaggcgct ggaccaaaat ctgtccatcg tcattgtggt caacaagatc gaccggcccg
     4681 acgcccgcat caaagaggtc atcgacgagg tgctgctact gctgatggat atgggcgcca
     4741 ccgacgagca gctcgactcc cccatggtct tctgctcggg ccgcgcgggc accgccagct
     4801 attcacccga cgcgcccggc accgacctga agccgctgtt cgacaccatc gtcgagtatg
     4861 tgaagccgcc ggtgggcgac cccaacgcgc ccctgcagtg cctggtgtcc tgcatcgact
     4921 acaacgaatt tgtgggccgc acctcaatcg gccgcatcga cgcgggcacc atgaaagtgg
     4981 gccagaacgt gaccgtctgc gactggcaca acaaggacct gtgcaaaaac gcccgcatca
     5041 ccgccctgta cgcctttgac ggcgtgggcc gcaaggagat cgaatccgcc tctgcgggcg
     5101 atatcgtgat gttctccggc atcgacgacg tcaccatcgg caacaccatc tgcgacgccg
     5161 gcgtgcccaa ccccatcccc tttgtcaaga t
//
LOCUS       DBMDYA010000112         5184 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3949_length_5184_cov_1.518395, whole genome shotgun sequence.
ACCESSION   DBMDYA010000112 DBMDYA010000000
VERSION     DBMDYA010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_5184_cov_1.518395"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="ACZPFK_03980"
     CDS             <1..963
                     /locus_tag="ACZPFK_03980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1405504.1"
                     /translation="LRAGEAVGIPTETVYGLAADATSAAAVKKIFEAKGRPQDNPLIV
                     HIARLEDLPAVVREIPEAAQKLAQAFWPGPLTIIMKKSDAIPMATSAGLDTVAVRMPS
                     HPVARQIIAASGLPLAAPSANLSGLPSPTTAQHTFQDMKGRIPLIVDGGPCEVGVEST
                     VITVAGETPTLLRPGYITREQLQEALGRRVALSSAILNKLAEGEVAASPGMKYKHYSP
                     RADVVMLSGAFPDYKKYVEAHAGEGVFAMCYEEEQPLLGVPCIPCGHAGDPASQAHAL
                     FAALREADERGARVVYAHSPAQDGVSMAVYNRLVRAAAFQVVQL"
     gene            960..1553
                     /gene="coaE"
                     /locus_tag="ACZPFK_03985"
     CDS             960..1553
                     /gene="coaE"
                     /locus_tag="ACZPFK_03985"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1405505.1"
                     /translation="MRVIGITGTSGSGKSTVSAWLAAQGYPVIDGDEVTRRLATPGSP
                     YVRALVKEFGPSVCDAQGNLLRRSLGERAFATPEGQKRLTAVTMPLILEEIERRIAAA
                     RAAGATLLFLDGALIIDTPFEPLCDRIVAVLSDRDTQARRIARRDGVPLRAALDRLDR
                     QASAAALRARADFVLENDGTRDELLQKAGQLLQQLKG"
     gene            1557..2141
                     /locus_tag="ACZPFK_03990"
     CDS             1557..2141
                     /locus_tag="ACZPFK_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR1405506.1"
                     /translation="MRKPRKNLIVLAALLAVGLLGLLTAPRAIRAIEERSFPLKYERT
                     VEQYAAEYQVDPLMVYAIIRTESGFDEAAESGVGARGLMQITEETFAWIKLKIANDEP
                     LTFDDLWDPSVNIRFGSYYLSRCLERYGGDLATASAAYHSGWGTVDGLLGDPAYAADE
                     STLKEFPYPQMSRYVQKVGHAFEKYERLYRQEQE"
     gene            2149..3546
                     /locus_tag="ACZPFK_03995"
     CDS             2149..3546
                     /locus_tag="ACZPFK_03995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1405507.1"
                     /translation="MEKEKSRAQQLADELLYQAPDVFEARPELKEAAWAFCEGYRHFL
                     NRAKTEREFVAGAVSLLQAAGYTLYEPGRIYRPGDKVYCVNRGKALLASTFGTLPVSE
                     GVRINAAHIDSPRLDLKQNPLYEKGNLSYFKTHYYGGLRKYQWTAIPLSLHGVFVRRD
                     GTAVPVCIGEEDGDPVFTISDLLPHLSKEQDRRPLADGVKGEELNILVGSLPYPDEEV
                     KNRVKLATMQLLHEKYGVTERDFTCAELEIVPAFPARDLGFDRSMIAAYGHDDRVCAY
                     AALRAEIDAKTPLHTTVTCLTDKEETGSDGNTGLASAYLLHYLEYLAEAQGVSDKAML
                     ANSKCLSADVNAAYDPTFADVFEENNVARLNKGVVVTKYTGARGKSGTSDASAETMAL
                     FADILDRHGVVWQTGELGKVDLGGGGTVAKYVANHNIDVVDIGVPLLSMHAPYEVAAK
                     ADIYMCYLAFSAFAE"
     gene            3728..>4723
                     /locus_tag="ACZPFK_04000"
     CDS             3728..>4723
                     /locus_tag="ACZPFK_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405508.1"
                     /translation="MALLKGTVKTALKLVLLLPLMLLVLWVNYFVDQTGLFRGGKYEL
                     ELAQILLGGDAVSNFSKMDEREVLKLYIKNMPEAYDTLVVGSSRGLQITAAIAGETGT
                     FYNAGMSGEDFYDVVGTVALLDKYDRLPKNMILVLDPWILNDNVDSYSKRSDADLANE
                     FLSETLGFHETYEAQDRSQYTEALLDLDYFQKNVAYYFEDHSDEDQPSRVSGNVYEQT
                     TETKMADGTLLYTREYRENSQEWVDFEALTLANGEALPFGFYDFVSLDERRCAELEAL
                     VEYLQNKNVEVTFVLSPFHPLVYNRFSEDENACGVLLAETFWKELASRCRIPVYGS"
     assembly_gap    4724..4733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1047 a         1492 c         1676 g          959 t
ORIGIN      
        1 ctgcgcgcgg gggaggccgt gggcatcccc accgagaccg tctacggcct ggccgcggac
       61 gccaccagcg ccgcggcggt gaaaaagatc tttgaggcca agggccgccc gcaggacaac
      121 ccgctcatcg tccatatcgc ccgcctggag gacctgcccg ccgtggtgcg ggaaatcccg
      181 gaggctgccc aaaagctggc gcaggctttt tggcccgggc cgttgacgat catcatgaaa
      241 aagagcgacg ccatcccgat ggccaccagc gcggggctgg acaccgtcgc ggtgcgcatg
      301 ccctctcacc cggtggcccg gcagatcatc gcggccagcg gcctgcccct ggccgccccc
      361 tcggcgaacc tttccggcct gccaagtccc accacggccc agcacacctt tcaggacatg
      421 aaaggccgca tccccctgat tgtggacggc ggcccctgcg aggtgggggt ggaatccacc
      481 gtgatcacgg tggcgggcga gacccccacc ctgctgcgcc cgggctatat cacccgcgaa
      541 cagctgcaag aggcgttggg gcgccgggtg gcgctctcct ccgccatcct caataagctg
      601 gcggagggcg aggtggcggc ctcgccgggc atgaaataca agcactattc cccccgggcg
      661 gacgtggtca tgctgtcggg ggcgttcccg gactataaaa aatatgtgga ggcccatgcc
      721 ggcgaaggcg tttttgcgat gtgctatgaa gaggagcagc ctctgttggg ggtgccctgc
      781 atcccttgcg gccacgcggg agatcctgcc agccaggccc acgcgctttt cgccgccctt
      841 cgggaggcgg acgagcgcgg cgcgcgggtg gtgtacgccc acagcccggc gcaggacggc
      901 gtctcgatgg ccgtctacaa ccggttggtg cgcgccgccg cttttcaggt ggtacagctg
      961 tgagggtgat cggcattaca gggacctcgg gcagcggcaa aagcacggtg agcgcctggc
     1021 tggccgccca gggatacccc gtcatcgacg gggacgaggt cacgcggcgg ttggccacgc
     1081 ccggcagccc ttatgtgcgg gcgctggtga aagagttcgg cccctcggtc tgcgacgcac
     1141 agggaaacct gctgcgccgc agtttgggcg agcgggcctt tgccacgccg gagggccaga
     1201 agcggctcac ggccgtcacc atgccgctta ttttagagga gatcgaaagg cggatcgccg
     1261 ccgcccgcgc ggcaggtgcg acgctgttgt ttctggatgg ggcgctgata atcgacacgc
     1321 cctttgagcc gctgtgtgat aggattgtag cggtgctgag cgatcgggac acacaggcgc
     1381 ggcgcattgc ccggcgggac ggcgtcccgc tgcgggcggc gctggaccgg ctggaccggc
     1441 aggccagcgc cgcagcgctg cgggcccggg cggatttcgt gctggagaac gacggcacca
     1501 gggacgagct gctgcaaaaa gccgggcaac ttcttcaaca gctaaaggga tgaccaatga
     1561 gaaaaccgag aaaaaatttg atcgtgctgg cggcgctgct ggccgtgggc cttttgggcc
     1621 tgctgaccgc ccccagggcg atccgcgcca tcgaagagcg cagcttcccg ctcaaatacg
     1681 agcgcacggt ggagcagtat gccgccgaat accaggtgga cccgctgatg gtctacgcca
     1741 tcatccgcac cgagagcggc tttgacgagg cggcggaatc cggcgtgggg gcgcgcggcc
     1801 tgatgcagat caccgaggag acctttgcct ggatcaagct gaagatcgca aacgatgagc
     1861 cgttgacctt tgacgatctg tgggatccgt cggtgaatat ccggtttggc agctattatc
     1921 tttcccgctg cctggagcgc tacggcgggg acctggccac ggccagcgcg gcctatcaca
     1981 gcggctgggg taccgtggac ggcctgctgg gcgacccggc gtatgcggcc gatgagagca
     2041 cgctgaaaga gttcccctat ccgcagatgt cccgttatgt gcagaaagtg ggacatgcat
     2101 ttgaaaaata tgaacggctt taccgccagg aacaggagtg agcaagggat ggaaaaagaa
     2161 aaaagcagag cgcagcagct tgcggacgaa ctgctctacc aggcgccgga tgtatttgag
     2221 gctcggcctg agctaaaaga ggcggcctgg gccttttgcg aggggtaccg gcactttttg
     2281 aaccgtgcaa agacggagcg cgagtttgtg gccggggccg tatcgctgct gcaggcggcg
     2341 gggtacaccc tgtatgagcc gggccgcatc taccggcccg gggacaaggt ctactgcgtc
     2401 aaccgcggca aggccctgct ggcctccacg ttcggcacac tgcccgtgag cgagggtgtg
     2461 cgcatcaacg cggcccacat cgattcgccg cgcctggacc tcaaacagaa cccgctctat
     2521 gaaaaaggca atttgtccta tttcaagacc cactactacg gcggcctgcg caagtatcag
     2581 tggacggcca tcccgctctc gctgcacggg gtctttgtgc gccgcgacgg cacggcggtg
     2641 cccgtttgca tcggtgaaga agatggggat cccgtcttca ccatcagcga cctgctgccg
     2701 catctctcca aagagcagga ccggcgcccg ctggcggacg gggtgaaagg ggaggagctg
     2761 aacatcctgg tcggctctct gccctatccc gacgaagagg tgaaaaaccg ggtcaagctg
     2821 gccaccatgc agctgctgca tgaaaagtac ggcgtcaccg agcgggattt cacctgcgcc
     2881 gagctggaga tcgtgcccgc gtttccggcg cgggacctgg gctttgaccg cagcatgatc
     2941 gcggcttacg gccacgacga cagggtgtgc gcctatgcgg cactgcgcgc tgaaatcgac
     3001 gccaaaacgc cgctgcacac caccgtgacc tgcctcaccg acaaggagga gaccggctcg
     3061 gacggcaaca ccggccttgc ctccgcctac ctgctgcatt atctggagta cctcgccgag
     3121 gctcaaggcg tcagcgacaa ggccatgctg gccaattcca agtgcctgtc cgcagatgtc
     3181 aacgcagcct atgaccccac ctttgccgac gtgttcgaag agaacaacgt cgctcgtctg
     3241 aacaagggcg tggtggtgac caagtacacc ggggcgcggg gcaaaagcgg cacctcggac
     3301 gcctcggccg agaccatggc tctctttgcc gatatcctgg acaggcacgg cgtggtgtgg
     3361 cagaccggcg agctgggcaa ggtggacctg ggcggcggcg gcacggtggc aaaatatgtg
     3421 gcaaaccaca atatcgacgt ggtggatatc ggcgtgcccc tgctgtcgat gcacgccccc
     3481 tatgaggtgg ccgcaaaggc ggatatttat atgtgctatc tggctttcag cgcttttgca
     3541 gagtaaggga gcgggcggct gaaagggtga aaacagcgcc gctgtaaggt actgcctgga
     3601 aaggttttgg gcatgcggcg cccacggcgg ccgtcttttg ggactttgcg tcgcaaaact
     3661 gtaaaaaatg tgatacaata ataaaacaaa gcaaaggggc ttgccctgga cggagggaac
     3721 acgacgcatg gccttgctca agggaacagt gaaaacagcg ctcaagctgg tgcttttatt
     3781 gccgctgatg ctgctggtgc tctgggtcaa ttattttgtg gaccagaccg ggctgttccg
     3841 gggcggaaaa tatgaactgg agctcgcgca gatcctgttg ggcggggacg cggtctccaa
     3901 ttttagcaag atggacgagc gggaagtgct taagctttac atcaaaaaca tgcccgaggc
     3961 ctatgacacg ctggtggtgg gatccagccg cgggctgcag atcacggccg ccattgcggg
     4021 ggagaccggc accttttata acgccgggat gtcgggcgag gatttttacg atgtggtggg
     4081 cacggtggcg ctgctggaca aatacgaccg gctgccgaaa aatatgatcc tggtgctgga
     4141 cccctggatc ttgaacgaca acgtggacag ctacagcaag cggtcggatg cggacctggc
     4201 caacgagttc ctcagcgaga cgctggggtt ccacgagacc tatgaggcgc aggaccgcag
     4261 ccaatacacc gaggcgctgt tggatcttga ctattttcaa aaaaatgtgg cctattattt
     4321 tgaggatcac tcggacgagg accagccctc ccgcgtgagc ggcaacgtct atgagcagac
     4381 cacagagacc aaaatggcgg acggcacgct gctgtacacg cgggagtatc gggagaacag
     4441 ccaggagtgg gtggactttg aagcgctgac gttggcgaac ggggaggcac ttccctttgg
     4501 cttttacgat tttgtgtccc tggacgagcg ccgctgcgcc gaactggaag cattagtgga
     4561 atatttacag aataaaaatg tggaagtaac ttttgtgctc agtccctttc atcctctcgt
     4621 ctacaacaga ttttctgaag acgaaaacgc ctgcggcgtt ctattggctg aaacattttg
     4681 gaaagaactt gcaagccgtt gccgtatccc tgtctacggc tccnnnnnnn nnncggctcc
     4741 tatgaccccg aacaaacggg ctgcacgaac gaagactttt atgacggttt gcatgtgcgc
     4801 agagaatcca tcaaaaagtt cttcttcggc attcacgctt ctctggtata gggctgggca
     4861 cccttttggc ttgaaagcca ggtgttacta ttgcgatgtg aacgcaccgt atcttaaaaa
     4921 tggaaagggc caagagcttt gctcttggcc ctttccattt ttctcctggg agtggagctg
     4981 cggggcaaaa ggcggcgggg acgccgcagc cgactgtttg acgcctgccc ctgtctcaac
     5041 ggcgtgtttg cctcgcgcga tggagagagg aggccgagct gccgtataat tttttttcaa
     5101 ctcttttcaa aactgatgta agattgtgct attttgcggc gttatccaca tataagaata
     5161 aggaaagaac cagtctgcgg tggc
//
LOCUS       DBMDYA010000113         5179 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3952_length_5179_cov_1.296377, whole genome shotgun sequence.
ACCESSION   DBMDYA010000113 DBMDYA010000000
VERSION     DBMDYA010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3952_length_5179_cov_1.296377"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="ACZPFK_04005"
     CDS             <1..600
                     /locus_tag="ACZPFK_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025668.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HIR1405509.1"
                     /translation="ETKEVTLTVPPRAFSFYSVAAQDFVVEQGRYELRVGSSSRKLPL
                     RAAVEAAGETGLRLPAAQLASAPYGALQDNWVPDAWFETLHPLPKQENRPARRGEFNR
                     TTPLGELAVTWPGRLMRRVAYRVGGATTHFVRSPKVNRRIVRRMTSEFPVKNIVLMSD
                     GLIDYDTVDALLDVCNGKGGWGRLLKGLGGALKKLRRRA"
     gene            complement(659..868)
                     /locus_tag="ACZPFK_04010"
     CDS             complement(659..868)
                     /locus_tag="ACZPFK_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405510.1"
                     /translation="MSKEKRAQSRREAVKKDHPSTVNINERIFAALETDELVPPSWPE
                     RPLDRAENGVDADGQNHFSFEEDDF"
     gene            1084..1159
                     /locus_tag="ACZPFK_04015"
     tRNA            1084..1159
                     /locus_tag="ACZPFK_04015"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1117..1119,aa:Pro,seq:ggg)
     gene            1552..2136
                     /locus_tag="ACZPFK_04020"
     CDS             1552..2136
                     /locus_tag="ACZPFK_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405511.1"
                     /translation="MSNNLIPRVSITTEMKKRAEEEAKKRDPHIKHHFDVSHFSGKVR
                     DVVGFLGEFACCEYLGLNWRDNIRENYLTIDTGDIQGRFILDIKTETLPEPYFSKLLN
                     HKLEDDEVYGRRLINGEQKALLHKYDFIVFGAFCRDRYDYWYPLGFMDTKFILQNYRA
                     TQNRPDGGSYPAAGLPIKTSELKPMEELRALFGQ"
     gene            2614..3120
                     /locus_tag="ACZPFK_04025"
     CDS             2614..3120
                     /locus_tag="ACZPFK_04025"
                     /inference="COORDINATES: protein motif:HMM:NF018410.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1405512.1"
                     /translation="MPVQTALVGAGGAVKSIFVVLSSMAPFVENKGSILLAAALNVKW
                     YLAYIGTSIGSFLPVPFLLRAGDRGMQRLRRFGPAARVLDQVEAFSRQHEAFLKRHGY
                     LALALIISVPFTGIGCWAGVLLSNMLGLDKKHSTAAILAGIIVSGFLTTAGAYGLFLG
                     IRSLIRLW"
     gene            3272..4735
                     /locus_tag="ACZPFK_04030"
     CDS             3272..4735
                     /locus_tag="ACZPFK_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1405513.1"
                     /translation="MKKFLSGLLVIAMAAGLFSGCGTGADAQRPSSKNPVTVTVWHYY
                     NGAQKNAFDLLVKEFNETVGLEQGIIVEAISQGDVNSLESSVLDSVEKKAGAAPVPNI
                     FAAYADNAYQVDQMGLLANLEDYLTEEELSQYIPSYIEEGRIGNDGGLKIFPTAKSTE
                     IFMLNKTDWDKFEAENDVSLDDLATIEGVVETARKYYEWTDAQTPGVADDGKAFFGRD
                     AMANYLLIGCRQMGVEIFSVKNGKVTFQADESVLRRIWDNYYVPFINGWFTAAGKFRS
                     DDAKVGNLIALVGSTASASYFPSEVTLGDADPYPIETLVLPAPVFEGGEPYAVQQGAG
                     MVVTKSTPQQEYASVEFLKWFTEEDRNLVFASLAGYLPVKRAANDPEKLETASIEGVQ
                     TQYTDLIRQTLDVALETTGTQPLYTSKAFAGGADARKVLEYALQDKAAEDRAAIDELV
                     AQGMSRSEAVAQFDTDENFAAWFADFSQRLEATQKGE"
     gene            4787..5179
                     /locus_tag="ACZPFK_04035"
                     /pseudo
     CDS             4787..5179
                     /locus_tag="ACZPFK_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
BASE COUNT         1183 a         1296 c         1536 g         1164 t
ORIGIN      
        1 gagacaaagg aggtcaccct gacggtgccc ccgcgggcgt tcagctttta ctccgtggca
       61 gcgcaggatt ttgtggtgga gcaggggagg tatgagctcc gcgtcggctc ctccagccgg
      121 aagctgccgc tgcgcgcggc ggtggaagcg gcgggggaga caggcctccg cctccccgcg
      181 gcgcagttgg cgtctgctcc ctacggcgct ctgcaggaca actgggtccc ggacgcctgg
      241 tttgagacgc ttcatcctct gcccaaacag gaaaaccgcc cggcgcggcg gggagagttc
      301 aaccgcacca cgccgctggg cgagctggcc gtcacctggc cgggcaggct gatgcggcgc
      361 gtcgcctacc gggtgggcgg ggccaccacc cactttgttc gctcgcccaa ggtgaaccgg
      421 cgcatcgtac ggcggatgac gtctgagttc ccggtcaaaa acatcgtgct gatgtcggac
      481 ggcctgatag attatgacac ggtggacgcc ctgctggatg tgtgcaacgg caagggcggc
      541 tggggacggc tgttgaaagg cctgggcggg gcgctgaaaa agctgcgccg gcgcgcttga
      601 cgggcaaggg gaaagtaagt aaaacggcgg aaagaggaag ctctctttcc gccgtttgtc
      661 aaaaatcatc ctcttcaaag gagaagtggt tttgcccgtc tgcatccacg ccgttttcag
      721 cccggtccaa cgggcgctcc ggccagctgg gaggcaccag ttcgtccgtc tccagcgcgg
      781 cgaaaatgcg ctcgttgatg ttgacggtgg aaggatgatc ctttttgacc gcctcgcggc
      841 ggctttgtgc acgtttttct ttgctcatca gtgttttaca cccccgcaag tattatatgt
      901 aaagagcggc ggcatattga ggcgcggccg ctggaagttt tttgaaactt tctgtcgaac
      961 gacctcccga cgcccctttc agagaaaaag ccgagagagc aaaatttttg gcaagaaatg
     1021 attgacaaaa gtttgggggt tcagtataat aataacgctg cctctgagag gcggcgttgt
     1081 tatccgggtg tggcgcagtt ggtagcgcgc ttgactgggg gtcaagaggc cgcatgttca
     1141 agtcatgtca ctcggaccat tgaaaagacc gtcgactttt gtcgacggtc ttttgttatg
     1201 cttatattca agctggtttg cagcgtgggc aataaatcaa agtcccctcg ttacaagaga
     1261 aatgcaatcc ttttccagaa ctcttttact atacgcttgt ttattttaga acgtgatctg
     1321 gcaggaatag acgacagatt gctatggcgg acttcactga attttctgtt tattttataa
     1381 ttgaaagtga ctgcagggat agaaatcgac tttttctttt ctatatggta tgatacaaaa
     1441 aacgaatttt cagacgaaat attttaaccg gcaaaaaaga agatcgactt gtcaaaccct
     1501 tgcggaacat ttttttcagc tttctatttg atatagaaaa gaggggtgca aatgtcaaat
     1561 aatttgatac ctagagtttc tatcacaact gaaatgaaaa aacgggcgga agaggaagca
     1621 aaaaagcgcg atccccatat caaacatcat tttgacgtct ctcatttttc aggcaaggtg
     1681 cgggatgtgg tcggcttttt aggcgaattt gcctgctgtg aatatttagg cctcaattgg
     1741 cgggataata ttcgcgagaa ctatttaacc atcgatacag gggatattca gggacggttt
     1801 atcctggata taaaaacgga aaccctgccg gagccctatt tttcaaagct tttgaatcat
     1861 aagctggagg atgatgaggt gtatggcaga cggctgatca atggggaaca aaaagccttg
     1921 ctgcataaat acgactttat agtctttggg gcgttttgtc gggatcgata cgattattgg
     1981 tatccgttgg gctttatgga cacaaaattt attcttcaaa attatagggc cacccagaac
     2041 agaccggatg gcggcagcta tcctgcggcc ggtctgccga tcaaaacttc cgagttgaaa
     2101 cccatggagg agttaagagc tttgtttggt caataggaaa tggaacttca acatgaagta
     2161 aaagccttgg aggcctcctc gattttgttt tctgtcgaaa gggctggcag ccgggtgaat
     2221 gggctcagta aaaatgagcg gcgatgcagt accccccgat gtaggaaaac agactacatc
     2281 agggggtact gtgttaagga acaagattac ccgcatgtcc aaacaccgct gagggagaga
     2341 aaggcaacag cacgaaacgg aaaaacgcag taagcagtaa aaagtagtga aacattgtga
     2401 gattaaggaa tattggcatt aaggaatagg tgtccaataa aagaagcctt atcctcaaaa
     2461 ccattcgctg accatatcgg cgtttgcgag ccgaaaagca tataaacttt acaaataaat
     2521 gccccagtgg tataatacag cacatcaaaa gagcagcggg gcctgaaggc cggctgttct
     2581 tgatgctttt tataaaaaca ggggggtgga ggaatgccgg tccaaacggc gcttgtcggc
     2641 gccggcggcg cggtcaaaag tatctttgtg gtgctctcct ccatggcgcc ttttgtggag
     2701 aacaagggct ctatcctgct ggccgcggcg ctgaacgtca agtggtatct cgcctatatc
     2761 ggcacctcca tcggttcttt tttgcccgtc ccctttctgc tgagggcggg cgaccgtggg
     2821 atgcagcggc tgcggcgctt tggccccgcg gccagggtgc tcgaccaggt ggaggcgttc
     2881 tcccgccagc acgaggcgtt tttaaaacgc cacggatatc tggccctggc ccttatcatc
     2941 tccgtcccct ttaccggcat cggctgctgg gcaggggtgc tgctctccaa tatgctgggc
     3001 ctggacaaaa agcattccac cgccgccatc ctggccggca tcattgtctc cggctttctc
     3061 accaccgcgg gggcctacgg cttatttttg ggcatccgca gcctgatccg tctgtggtga
     3121 catctgtttt tgcgccgccc ccaccgtttt gccgggaggg cggcgttttc cattcttttc
     3181 ttgctaagag gtcacaaaag gaatataatt gtgctaaata agcacgcgtt ctgtttccac
     3241 ggggaagcga gagacgcggt gggaggaaaa gatgaaaaag tttttgtcgg gcctgctggt
     3301 cattgcgatg gccgccggcc tttttagcgg ctgcggcaca ggggcggacg cgcagcgccc
     3361 cagcagcaaa aacccggtga ccgtcaccgt ctggcactat tacaatgggg cgcagaaaaa
     3421 tgcattcgac ctgctggtaa aagagttcaa tgagacggtg gggctggagc aggggatcat
     3481 cgtcgaggcc atcagccagg gcgatgtgaa cagcctggag agcagcgtgc tggattcggt
     3541 cgaaaaaaag gccggggcgg ccccggtgcc gaatatcttt gccgcctatg cggacaacgc
     3601 ctatcaggtg gatcaaatgg ggctgctggc caacctggag gattacctca ccgaggagga
     3661 actcagccag tacatcccct cctatatcga ggagggccgg atcgggaacg acggcggctt
     3721 aaaaatcttc cccaccgcaa aatccaccga gatcttcatg ctcaacaaga ccgactggga
     3781 caagttcgag gcggaaaacg atgtgtccct tgacgatctc gccacgatag agggcgtggt
     3841 ggaaaccgcc cgaaaatatt acgaatggac cgacgcccag acgcccggcg tggcggacga
     3901 cggcaaggcg tttttcgggc gcgatgccat ggcgaattat ctgctgatcg gctgccgcca
     3961 gatgggggtc gagatctttt cggtaaaaaa cggcaaggtc acgttccagg cggacgagag
     4021 cgtgctgcgc cgcatctggg acaactatta tgtccccttt atcaacggct ggtttacggc
     4081 ggcgggtaaa ttccgctccg acgacgccaa ggtgggcaac ctgatcgccc tggtgggctc
     4141 caccgcctcg gcctcctatt tcccctcgga ggtgacgttg ggggatgccg atccctatcc
     4201 catcgagacg ctggtgctcc cggcgccggt ctttgagggc ggcgagccct atgcggtgca
     4261 gcagggcgcg ggtatggtgg tcaccaaatc cactccacag caggaatatg ccagcgtgga
     4321 gttcctcaaa tggttcacgg aagaggatcg caatctggtg ttcgccagtc tggcgggata
     4381 tctgccggtg aagcgggctg caaacgaccc cgaaaagctg gagaccgcca gcatcgaggg
     4441 ggtacagacc cagtacacgg acctgatcag acagaccctg gatgtggcgc tggagacgac
     4501 cggcacccag ccgctttaca ccagcaaggc gtttgcgggc ggggcggacg cgcgcaaggt
     4561 gctggaatat gccctgcagg ataaggccgc tgaggatagg gctgccatcg acgagctggt
     4621 ggcacagggc atgagccgca gcgaggcggt ggcacagttc gatacggatg aaaattttgc
     4681 ggcctggttt gctgattttt cgcagcggct ggaggcgacg cagaaaggag aatagcccat
     4741 gaaacgcgca aaggcgtctc tgttcaaaaa gatcgttttc ccgatcctgc tggtcatgct
     4801 gttccaggcg gttctgcttg cgcatggggg actgacaacg ctgggagcca ataccttttc
     4861 catggcgatt gcaggaccgt tcgttactta tggaatttat ctgctctgca aaaaatgtag
     4921 ggtgaacagg atggccggga tttttctcgc cgcttttttc ggggatcttt ttacttattg
     4981 tgtaacgggc atacagcttg cgctggcata tcccagcacg cagggaggcg tatttgcgtc
     5041 cgccctggaa ttcctcggcg tgtttgcgcc gacccagctg ccgctggccg ttgttgaagg
     5101 cctgctgacc gtgctgatta tgatggcgct ggaaaactat gcgaagccgg agttaaaggc
     5161 aatcggtttt atgaaggcc
//
LOCUS       DBMDYA010000114         5178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3953_length_5178_cov_1.557810, whole genome shotgun sequence.
ACCESSION   DBMDYA010000114 DBMDYA010000000
VERSION     DBMDYA010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_5178_cov_1.557810"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="ACZPFK_04040"
     CDS             complement(<1..570)
                     /locus_tag="ACZPFK_04040"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1405514.1"
                     /translation="MEQVITVKAPASVANLGSGFDTMGIALSLFNTVEMREWDGVEVS
                     TTDGTEPVQGEENLIYRTVKAVYDECGRTLPGLKLVQASPIPQARGLGSSSACIAAGV
                     VGANRLLGGVLTEREQLSLAARLEGHPDNVVPAMLGGFVTSVMEGGKVYTVKKKGSEE
                     LLFCAFVPDFELLTEKARAALPQQVPHRDA"
     gene            complement(575..1804)
                     /locus_tag="ACZPFK_04045"
     CDS             complement(575..1804)
                     /locus_tag="ACZPFK_04045"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1405515.1"
                     /translation="MIKIAILGFGTVGSGVWEVLRKNAVSVAKRAGEPVQVKYILDIR
                     DFSSHPDAALFVNSIDPILEDPEVDTVVETIGGTKPAYFYVKSALEAGKNVCTSNKEL
                     VATHGAELLALAVRHKVCFLFEASVGGGTPVITPLHQCLAANVISEVCGIVNGTTNFM
                     LTKMEQEGMSFDEALKVAQGLGYAETIDPSADVDGIDACRKIAILASLIFGSEVHPEN
                     IPTRGIRAVTEEDVYFTKKMKLAVKLIAYARRNEKAGFACGVEPMVLPAESMMAGVSD
                     VYNAVLVRGDMLGDTLFYGKGAGKLATASAVVADVIDAAKFGPSVHDSLFWAPTAPLK
                     GKITDEAVYTYYVRVNNLDAETLKADLGGVVLTLQHHNQTAVLTTPMTAKELEKRREK
                     LERDGIHVAIALRMLEV"
     gene            complement(1893..2327)
                     /locus_tag="ACZPFK_04050"
     CDS             complement(1893..2327)
                     /locus_tag="ACZPFK_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1405516.1"
                     /translation="MEKKFLIVDQAVLPEVFSKVLRAKELLASGAAKNVSAAAKMVDL
                     SRSAFYKYKDSVFDFENTKSIVTVKAVLLDETGALQALLSELSASGASIVTINQSAPE
                     NGTAVVSVTLRTDSMPQPIDDVLLSIQGQRTVVSIRRTVKEL"
     gene            complement(2602..4785)
                     /locus_tag="ACZPFK_04055"
     CDS             complement(2602..4785)
                     /locus_tag="ACZPFK_04055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1405517.1"
                     /translation="MWANIGRVLVVLVSIGVIVASVLAVMLSLYVVKVTANDATLLSL
                     ENLKLSYSSTIYAINAQTGEPEPYQRLVDNADRIWVDGNQIPDNLKNAFIAVEDKTFR
                     ENNGVNFKRTVSATANLVLSKLTGGKIKLYDTEQGASTITQQLIKNITGDDDHDALRK
                     VREIFRAISLTGRYSKDTILEAYLNTVGFMGNTCGVQACAYNLYDKDVSQLTLAQCAT
                     VAAITNNPTKYNPRTEPENNLQRRDMILGMMLEQGLITQAEHDAAVAEPLNLIDPAEK
                     QNTAKVGNNSYFTDMVIDEVITDLVSQGICETRSEASALLYNGGLAIYATVDTNLQSI
                     MEEVMAKEPGNYYPDREVEYEDKATGETKTQNVQGAMITIDYTGAVKGVVGGLGEKTA
                     DRGLNRAVDSLRQTGSTMKPIGAYAMGIDYNYITYSDGGFIDDWVEEINDNGTMRKWP
                     RNYNGKYTEQRMLVCDALANSINTIAVRVGKLVGPDDMFDFMTNTLGITSLVESRTEA
                     NGKISTDIGVGPLVLGSMTDGVSPAEMAHAYMMFGDGGTVTPLHSYTTVENVQTGEVL
                     LDNTLLPTTRAIGEDTAMIMNKLLRQVMTRGTARTTNSAQIAGMETVGKTGTTSEDVD
                     HWFIGLTPYYVTAIWMGYDQPQELPWKGNQYSQHPPTLAWKEVMERAQEGLEAKNFPT
                     APAGTVVTQAYCTESGGAAGPLCPKTATGYYKADQLPEPCDIHVG"
BASE COUNT          842 a         1617 c         1598 g         1121 t
ORIGIN      
        1 cgcgtcccgg tggggtacct gctgcggcag ggcggcccgc gctttttcgg tgagcagctc
       61 aaaatccggc acaaaggcgc agaacagcag ctcttcactg cccttttttt tgaccgtata
      121 gaccttgccg ccctccatca cgctggtgac aaagccgccc agcatggcgg gcaccacgtt
      181 gtcggggtgc ccctccaggc gcgccgccag cgagagctgc tccctttcgg tcagcacgcc
      241 gcccagcagc cggttggcgc ccacgacccc cgccgcgatg caggcgctgc tgctgcccag
      301 gccccgggcc tgggggatgg ggctggcctg caccagcttc agccccggca gagtgcggcc
      361 gcactcatcg tacaccgcct tgacggtgcg gtagatgagg ttttcctcgc cctgcaccgg
      421 ctcggtgccg tcggtggtgg acacctccac gccgtcccac tcccgcatct cgaccgtgtt
      481 gaacagcgag agcgcgatgc ccatggtatc gaagccgctg cccagattgg ccaccgaggc
      541 cggggccttt accgttatca cctgttccat gctcttacac ttccagcatc ctcagcgcga
      601 tggccacgtg gatgccgtcc ctttccagtt tttccctgcg cttttccagc tcctttgccg
      661 tcatcggggt ggtgagcacc gcggtctggt tatggtgctg cagcgtgagc accacgccgc
      721 cgaggtcggc tttcagggtc tcggcgtcca ggttgttcac tctcacataa tatgtataaa
      781 ccgcctcgtc ggtgattttg cctttcagcg gcgcggtggg cgcccagaac agcgagtcgt
      841 gcaccgacgg gccgaacttg gccgcgtcga tgacatcggc caccacggcg ctggcggtgg
      901 ccagcttgcc cgcgcccttg ccgtaaaata gtgtgtcgcc cagcatgtcg ccccgcacca
      961 gcacggcgtt atagacgtcg ctgacccccg ccatcatgct ctccgcgggc agaaccatag
     1021 gctccacgcc gcaggcaaag cccgcttttt cattgcgccg ggcataggcg atcagcttga
     1081 cggccagctt catctttttg gtgaaataga cgtcctcctc cgtcaccgcg cggatgccgc
     1141 gggtggggat gttttcggga tgtacctcgc tgccgaaaat aagggaggcc aaaatggcga
     1201 tcttgcggca ggcgtcgatg ccgtccacat ccgccgaggg gtcgatggtc tcggcatatc
     1261 ccaggccttg ggccactttc agcgcctcgt caaagctcat gccctcctgc tccattttgg
     1321 tgagcatgaa gttggtggtg ccgttgacga tgccgcagac ctcgctgatg acgttggccg
     1381 ccaggcattg gtgcagcggc gtgatgaccg gtgtgccgcc gcccaccgac gcctcaaaca
     1441 ggaagcagac cttgtgccgc accgccaggg ccagcagctc ggcgccgtgg gtcgccacca
     1501 gctctttgtt ggaggtgcag acgtttttgc ccgcctccag cgccgacttg acataaaaat
     1561 aagcgggctt ggtgccgccg atcgtctcca ccaccgtgtc cacctcggga tcctccagga
     1621 tgggatcgat gctgtttaca aacagcgccg cgtccgggtg ggacgaaaaa tcgcggatat
     1681 cgaggatata cttcacctgt accggctcgc ccgcgcgctt ggccacgctg accgcgtttt
     1741 tgcgcagcac ctcccagacg ccgctgccca ccgtgccgaa gcccaaaatt gctatcttga
     1801 tcaaaacatc tcaccttttc tttctttttt gatgccggat taccggccgg atggcctggg
     1861 gacaagggcc gcccgggccc cgcagggcgc cgctacagct ccttgaccgt gcgccggatg
     1921 ctcaccaccg tgcgctggcc ctggatcgag agcagcacat cgtcgatggg ctgtggcatt
     1981 gaatcggtgc gcagcgtgac cgagacgacg gcggtgccgt tttccggcgc ggactggttg
     2041 atggtgacga tgctggcgcc ggaggcggac agctcgctca gcagggcctg cagagccccc
     2101 gtctcgtcca gcagcaccgc cttgaccgtg acgatggatt ttgtgttttc aaaatcaaaa
     2161 acgctgtcct tgtacttgta aaacgcgctg cgggagagat cgaccatttt agccgcggcg
     2221 gaaacgtttt tcgccgcgcc gctggcgagc agctcttttg cccgcagcac cttggaaaag
     2281 acctccggga ggacagcctg atccacgatc agaaattttt tctccacgtt tttgtccgcc
     2341 tttcaaacac atttgtttac accgtcgcca ctatatcact tttttctcca gcgggcaagc
     2401 aactttttca agattttccc tgttgcacaa aaccgctctg gcccggcgag ctcgggaggg
     2461 tattttgacg aaatcatctg ttctctgtaa aaacacaaaa acgccctgtg agatcttcac
     2521 agggcgtttt gaaagacaag ttgtgttcca gccaaaagcg gagcggccgt cgccgtgggg
     2581 caatggggcc cggcggaccg gtcagcccac gtggatgtcg cagggttccg gcagctggtc
     2641 ggccttgtag tagccggtgg cggttttggg gcagagcggc ccggccgcgc cgccggactc
     2701 ggtgcagtag gcctgggtga ccaccgtgcc cgccggggcg gtggggaagt tcttggcctc
     2761 cagcccctct tgcgcccgct ccatgacctc tttccaggcc aacgtgggtg gatgctgact
     2821 atattgattg cccttccagg gcagttcttg aggctggtcg taccccatcc agatggcggt
     2881 gacataatag ggggtcagac cgatgaacca gtggtcgacg tcctcggaag tggtaccagt
     2941 tttgcccacc gtctccatgc cagcaatctg ggcggaattg gtggtgcgcg cggtgccccg
     3001 cgtcatcacc tggcgcagca gcttattcat gatcatggcg gtgtcctcgc cgatggcgcg
     3061 ggtggtgggc agcagggtgt tgtccagcag cacctcgccg gtctgcacgt tttccaccgt
     3121 ggtgtaggag tgcagcgggg tgacggtgcc gccgtcgccg aacatcatat aggcgtgggc
     3181 catctcggcc ggcgagacgc cgtcggtcat ggagcccagc accaacggcc cgaccccgat
     3241 atcggtagaa attttcccgt tcgcctcggt cctgctctcc accaggctcg tgatgcccag
     3301 ggtgttggtc ataaagtcga acatgtcgtc gggccccacc agcttgccga cccgcaccgc
     3361 gatagtgttg atggagttcg ccagcgcgtc gcagaccagc atgcgctgct cggtgtactt
     3421 gccattgtag ttgcggggcc atttgcgcat ggtgccgttg tcgttgatct cctccaccca
     3481 gtcgtcgata aagccgccgt ccgagtaggt gatatagttg tagtcgatgc ccatggcata
     3541 ggcgccgatg ggcttcatgg tggagccggt ctgccgcagc gaatccaccg cgcggttgag
     3601 accgcggtca gcggtctttt cgcccaggcc gcccaccacg cccttgacgg cgccggtgta
     3661 gtcgatggtg atcatcgcgc cctgcacgtt ctgggtcttg gtctcgccgg tggccttgtc
     3721 ctcgtactcc acctcccggt ccgggtaata gttgcccggc tccttggcca tgacctcctc
     3781 catgatggac tgcaggttgg tgtccacggt ggcgtagatg gcgagaccgc cgttgtacag
     3841 cagggcgctg gcctcgctgc gggtctcgca gatgccctgg ctcaccaggt cggtgatgac
     3901 ctcgtcaatg accatatcgg tgaaatagct gttgttgccc accttggcgg tattctgctt
     3961 ttcggcggga tcgatcaggt tcagcggctc cgccaccgcg gcgtcgtgct ccgcctgggt
     4021 gatcagcccc tgctccagca tcatgcccaa aatcatgtcg cggcgctgca ggttattctc
     4081 cggctcggtg cgcggattgt acttggtggg gttgttggtg atggccgcca cggtggcgca
     4141 ctgggccagg gtgagctggc tgacatcctt gtcgtacagg ttgtaggcgc aggcctgcac
     4201 gccgcaggtg ttgcccataa agcccaccgt gttgagatag gcctcaagga tggtgtcctt
     4261 ggagtagcgg ccggtgagcg agatggcgcg aaagatctcc cgcaccttgc gcagcgcgtc
     4321 gtggtcgtcg tcgccggtga tgttcttgat cagctgctgg gtgatggtgg aagcgccctg
     4381 ctcggtgtcg tacagcttga tcttgccgcc ggtgagcttg ctgagcacca ggttggcggt
     4441 ggccgagacc gtgcgcttga agttgacgcc gttgttctcg cggaaagtct tgtcctccac
     4501 ggcgataaag gcgtttttca ggttgtcggg gatctggttg ccgtccaccc agatgcggtc
     4561 agcgttgtcc accaggcgct ggtagggctc tggctcgccg gtttgcgcgt tgatggcgta
     4621 gatggtggag gaatagctga gcttcagatt ttcaaggctg agcagcgtgg cgtcgtttgc
     4681 cgtcaccttg accacataaa ggctgagcat gactgccagc accgaggcca cgatgacccc
     4741 gatgctgacc agcaccacca ggacgcggcc gatgttggcc cacacccttt tggcgccgcc
     4801 tttctttttc ggtttttgag gggcgccgcc ctgcgcgcct gcagcgcgcg cgtgagaaga
     4861 gctgccgccg ctgccccgcg cgggggcgcc gcgccggggc gccggtttcc ggttctgttc
     4921 gttcaaaaag gttcctcccc tttcgtctca tcagcagtct tgtctgaact atgtaaattt
     4981 aacggagaag cactaagggc cggcggtctt tcgccgggtt tctatagtat accacaactt
     5041 tcccctccat ttccactttt cacagattac aaaatgaaaa cggcgggggc ggccacacgc
     5101 ataaccggcg cgcttttctc ccacaatagg gttgttcggg gccggtccga tccagtccag
     5161 aggcccccgg gaggtgcg
//
LOCUS       DBMDYA010000115         5128 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_3997_length_5128_cov_1.731848, whole genome shotgun sequence.
ACCESSION   DBMDYA010000115 DBMDYA010000000
VERSION     DBMDYA010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3997_length_5128_cov_1.731848"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            407..1597
                     /locus_tag="ACZPFK_04060"
     CDS             407..1597
                     /locus_tag="ACZPFK_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1405518.1"
                     /translation="MKGKNKWLWSHRTLANLFVVLVGIAFYLCLSNFDLVRGKVAEIV
                     KIFTPFIAGFALAYLLNLPMRFFERRVFRRFRARRGLSILTSYLLAAVLLAVLLGLVL
                     PQLVQSVMALAANVPAYLRNLNDLLDFLIDKFHVDKEIVSQLGMTYKEIVQTLTAAVT
                     AAAPQLLNFSMALGNGVVTGLTALISSIYMLAGKDKLIFQLKKVVYALMPTPRARRFL
                     SVCDHANRVFAGFINGKLIDSAIIGVLCFVCMSLLRMPLALLISTVVGVTNVIPFFGP
                     FIGAIPSVMILLIVDPWSALWFGILIILLQQFDGNILGPKILGDSTGLSAIWVLVAII
                     VGGGLFGFAGMLLGVPAFAVLYSLSSDFIAARLAARGVDAAGEPARPAGEKTDEKTEE
                     ERAQ"
     gene            1594..2091
                     /locus_tag="ACZPFK_04065"
     CDS             1594..2091
                     /locus_tag="ACZPFK_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405519.1"
                     /translation="MKSKRRALLKILGALALVGVAALASLLIKETIDSTDPENNLPLI
                     EASVGYTKLNVVRANFEWNYITRKVRGPGLSAESGDVPFAPLDVIPDSKLVVVMSDPN
                     YLSISVSRADGMYDADYIEIYGDVFTPKTPGVYYYKIEVSYKKGSLLYYTAVRVQDPG
                     QLTVG"
     gene            2368..3051
                     /locus_tag="ACZPFK_04070"
     CDS             2368..3051
                     /locus_tag="ACZPFK_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1405520.1"
                     /translation="MMKNYAVRTARLLFGLALFGFGCYLSIQANIGLAPWDAFSMGFA
                     KLTGQSYGNMTVLTGLGILVVDCLLGEKIGVATVLNTLLVGKFVDLFDALGLVPKLDN
                     FFAGVALLLAGQVVLCLGSYFYIGAAMGCGPRDSLMVGVGKKLSRLPIGLVRGGVEAA
                     ALLVGWLMGAKVGLGTVIAVFGIGFLLETTFRLLHFDVKAVVHENLFETARRLAGKEN
                     KNDLEKKEL"
     gene            complement(3224..3640)
                     /locus_tag="ACZPFK_04075"
     CDS             complement(3224..3640)
                     /locus_tag="ACZPFK_04075"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR1405521.1"
                     /translation="MEKYSAILPPLKTNEDLALSDEEFSRQASLRALTACSSFSRTSQ
                     GFAADTTLLRDNLLFFSIPFEKGWRAEVDGTPTQIEKVDFGFMAIPLPAGQHHIELFY
                     TSSGQRAGTVLSVTSGLVLIFCCCFSLQSQKRKKRQ"
     gene            complement(3644..5068)
                     /locus_tag="ACZPFK_04080"
                     /pseudo
     CDS             complement(3644..5068)
                     /locus_tag="ACZPFK_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
BASE COUNT         1221 a         1292 c         1515 g         1100 t
ORIGIN      
        1 tccggcgcgc ggcgccggtt tacctttcag tgtatgccaa cggaaaagaa aaaatgagag
       61 aaaaagcgcg cggcggccgg gagatgccga aaaaagcggt ctcgaccttg ggccggggcc
      121 ccggcccgac ggctttgacc cccgtcccac cgccgcggct ttgaccctca tcctgctgcg
      181 ccggctttga cccccgtccc accgccgcgg ctttgaccct catcctgctg cgccggcttt
      241 aacccccgcc ccgctgctgc ggctttggtt tggccgtggc gcgcggggcg gcacggctgc
      301 cttttgggtg gatttttgag cgggcatggt atataatgtt tctaagcctg tccggcctgg
      361 ttcaaagatc gccgcagtag ggggacagaa gagaggggac gcgccgttga aagggaaaaa
      421 caaatggctg tggagccacc gcacgctggc caacctgttt gtggtgctgg tggggatcgc
      481 gttctatctc tgcctgtcga attttgatct ggtgcggggc aaggtggccg agatcgtcaa
      541 gatcttcacc ccgttcatcg cggggtttgc gctggcctac ctgctcaatc tgcccatgcg
      601 cttttttgag cgcagggttt ttcgccgctt ccgggcgcgg cggggcctgt ccatcctcac
      661 ctcgtacctg ctggccgcgg tgctgctggc ggtgctgctg ggcctggttc tgccccagct
      721 ggtgcagagc gtaatggccc tggcggccaa tgtgcccgcc tacctgcgca acctcaacga
      781 tctgctcgat tttttgatcg acaagttcca cgtggataag gagatcgtct cccagctggg
      841 gatgacctat aaggagatcg tgcaaacgct caccgcggcc gtgacggcgg ccgcgccgca
      901 gctgctcaat ttctcgatgg cgctgggcaa cggcgtggtg accgggctca ccgccctcat
      961 cagctctatc tacatgctgg cgggcaagga taagctgatc ttccagctca aaaaggtggt
     1021 ctacgcgctg atgcccacgc cgcgggccag gcgctttctc agcgtctgcg accacgccaa
     1081 ccgggtgttc gcgggcttta tcaacggcaa gctcatcgac agcgccatca tcggggtgct
     1141 gtgctttgtc tgcatgagcc tgctgcgcat gcccctggcg ctgctcatca gcacggtggt
     1201 gggcgtcacc aatgtgatcc cctttttcgg gccctttatc ggggcgatcc ccagcgttat
     1261 gatcctgctg atcgtggacc cctggagcgc actctggttc ggcatcctca tcattttgct
     1321 gcagcagttc gacggcaaca tcctgggccc caagatcctg ggggattcca ccggcctgtc
     1381 cgccatctgg gtgctggtgg cgatcatcgt gggcgggggg ctgttcgggt ttgcgggcat
     1441 gctgctgggg gtgccggcct ttgcggtgct ctacagcctg tccagtgact tcatcgccgc
     1501 caggctggcg gcgcggggcg tggacgccgc cggggagccg gcgcggcctg cgggggaaaa
     1561 gacagacgaa aaaacagaag aggagagggc ccaatgaaga gcaaaagacg ggctctgctg
     1621 aaaattttgg gcgcgctggc gctggtgggg gtcgccgcgc tggcgagcct gctcatcaag
     1681 gagaccatcg actccaccga cccggagaac aacctgccgc tgatcgaggc ctcggtgggc
     1741 tacaccaagc tcaatgtggt gcgggccaat tttgagtgga actatatcac ccgcaaggtg
     1801 cgcgggccgg ggctgtcggc ggagagcggc gacgtgccct ttgccccgct ggacgtgatc
     1861 cccgactcaa agctggtggt ggtgatgagc gaccccaact atctctcgat cagcgtctcc
     1921 cgcgccgacg ggatgtacga cgccgattat atcgagatct acggcgatgt gttcaccccc
     1981 aagacgccgg gcgtttatta ctacaagatc gaggtcagct acaaaaaggg cagcctgctc
     2041 tattacaccg cggtgcgggt gcaggacccg ggccagctga cggtggggta aagcccgcgc
     2101 cctgcggaaa acgaacagaa aagagcggcc gtcggatttt tcggcggccg ctcttgcttg
     2161 ttttttgggt ggtgaaagaa gccgcttgcg gtcccgcgct ttcacagcgc tccggcctcg
     2221 gccggggcgg tgggaacacc cgggcgggtg cgcgtctgcg gtatagaggg aattttgccg
     2281 caaaacggcg attttgccgc aaaactgtgt tacaatagaa agcgatctcc ccgccgaaag
     2341 aagcgggcga taaggagttg tggatcgatg atgaagaatt atgcggtgcg gacggcgcgg
     2401 ctgctgttcg gtcttgccct gttcggcttt ggctgctatc tgagcatcca ggccaacatt
     2461 ggcctcgccc cgtgggacgc cttttccatg gggtttgcca agctgacggg gcaatcctac
     2521 ggcaacatga cggtgctcac cggcctgggc atcctggtgg tggactgcct gctgggcgaa
     2581 aagatcggcg tcgccaccgt gctcaacacg ctgttggtgg gcaagtttgt ggatctgttc
     2641 gacgcgctgg ggctggtgcc caagctggac aacttttttg cgggcgtggc gctgctgctg
     2701 gcggggcagg tggtgctctg cctgggcagc tatttctaca tcggggcggc catgggctgc
     2761 ggcccacgcg attcgctgat ggtcggcgtg ggcaaaaagc tgtcccggct gcccatcggt
     2821 ctcgtgcgcg gcggggtgga ggccgcggcc ctgctggtgg gctggctcat gggggcaaag
     2881 gtgggtctcg gcactgtaat cgcggtgttc ggcatcggct ttttgctgga aacgactttc
     2941 cggctgctgc attttgacgt caaggcggtc gtgcatgaaa acctgttcga gacggcccgg
     3001 cggctggcgg ggaaagaaaa taaaaacgat ttagagaaaa aagagcttta gaaaggacga
     3061 cgaggctgac cgaaagtttg taaaaagcgt tctgtgtatg gttgtcgtcc agactacctt
     3121 atacagaacg cttgtgttat gaaacgatga gaaaaaataa gtttttcctt tccaattcgg
     3181 ttagagaaat ttgtgaaagg aagacggttt tacggacgac ttattattgc cgcttttttc
     3241 ttttttggga ttgaagactg aaacaacagc agaaaattag cacgaggcca ctggtaacgg
     3301 aaagaacagt accggctctc tgacctgaag aagtatagaa aagttcgata tgatgttggc
     3361 ctgccggaag aggaatggcc ataaagccaa agtcaacttt ttcaatttgg gtcggggtac
     3421 cgtcgacttc tgccctccac cctttttcaa aaggaatact aaaaaacaga aggttgtccc
     3481 ggagtaaagt ggtgtctgcg gcaaagcctt gcgacgtgcg ggaaaacgag ctgcaagctg
     3541 taagggcgcg cagagatgcc tgacgggaaa attcttcgtc ggaaagagcc aggtcctcat
     3601 tcgtcttaag cggtggcaag attgccgaat acttttccat ttgctccggg gtaagaatta
     3661 acgcccgcac cagcgcacgg ctgcgcgtat ttacattttc gagagattga aattgttccg
     3721 gagtcatact gttgtcgaaa gtatatccca tagggataaa attactgttt tgataaatga
     3781 taaaatcctg ttgttgggaa acaatctcgg aacccggcat agaattgata aaagagggct
     3841 caccgatact gtgtaccaga tactttacac ttaaaaaaga acgaagaccg tcgttttccc
     3901 aaagaggata gctgcgaatt gtacgtggta tccctaatgc ctcataaaaa tcaaagatag
     3961 acccgctgac cgtacttgaa aaactagtca tcgtagggca atctaaataa agacccagat
     4021 tgagaaaact atgggccgag tccatgcgcc aattgtcgtt ttcatcctca aatttcaaag
     4081 atttggagct gataaaaagc tgctttggag aaaggccggc attttgtgac cagtatttct
     4141 gttcataatg gatatttata aagccggttc ccgcgctgaa aaaaatgacc aaaagggtca
     4201 gtaaaactgt accttggcgc agagactgaa gcttgcgtaa cgatgcacaa accattaatc
     4261 cggctaaagc gattatcaca aaaacaatag ccagaaaaag gttgacgata aactgagagt
     4321 gataaaagaa cttccataca ccccaagtaa gaaaaaacaa aattgtgacg cagccataag
     4381 ccaaaaagcc tggacgtaga gagagatcag gcgcctctaa agcgtgaacc gttgcgaggc
     4441 agagaatcaa aataggcata tagtgccagc gcggatagta ttcggcggaa ccagccatga
     4501 aaatactatt taaaactgga atgaaaacca tgacggcgca caagaacaaa aggcgggtaa
     4561 tccatgattt tcgatggctc caacaccagg caaaagcgag gattaagccg aaaacaggga
     4621 gataaaattc tggaatatta gagatactgg gaagaaagaa agcccgacta gcaatcgcct
     4681 ccgcaggaaa caaaagagat ctcaaaaggt ccgcaaactg tagtggagga taaagaagca
     4741 tagaaaaaaa gctgtcgaaa tgttctccga tgcgagggtt ttgaaggagt gaaagaatgg
     4801 ttggaagtaa aagaacaccg gtaaggccaa caccaaagat gctttcgatc gccaaacgac
     4861 caaacaaagc ccaatcaagc cggtatgttc cgcaccaggt cataacaata aaataaagaa
     4921 ctaaaaagac gactgaaccc acaaaaaaga cactgttggt aaataaaagg agagcgaccg
     4981 ttaaggcgaa aattcctaag gggagttttt tgcgtagttt tacggtttga tctagggcca
     5041 aaagtaaaag agggaaaagg gccgttacgt ctgcgaaatg gttgaagttg aggtaacggc
     5101 ccttttccct cttttacttt tggcccta
//
LOCUS       DBMDYA010000116         5122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4003_length_5122_cov_1.605059, whole genome shotgun sequence.
ACCESSION   DBMDYA010000116 DBMDYA010000000
VERSION     DBMDYA010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_5122_cov_1.605059"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="ACZPFK_04085"
     CDS             complement(<1..308)
                     /locus_tag="ACZPFK_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405522.1"
                     /translation="MLERIEKYLEATTLIPALAGHEEKLAAYLRREFEACGLPVQTDA
                     LGNCMARIEGTDPSAPVVMVFAHMDSLGFVVRYIEEDGFIRIERLGGIPEKVLPATEI
                     "
     gene            complement(358..1149)
                     /locus_tag="ACZPFK_04090"
     CDS             complement(358..1149)
                     /locus_tag="ACZPFK_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1405523.1"
                     /translation="MENGKIMFQAERQERILEYINTNKKVRTKELSRIFNTSMVTIRA
                     DLNDLDEKGMIIKAHGGAMAISDRLNLEIPSQSKSRHNIDAKRAIARQAADLVKENDV
                     VILDAGSTTLEIAKLIKNKAITVITNDLKIGVTLAPSGSVTLIMTGGTVEPMVYTLCG
                     METVEFLRGIKANKMFLGCDAVDPRKGVSNRTLLEVGVKKAMIQASEQVIAVADGSKH
                     GKQVFAHVCDASDLDVLVTDRIPRKEKEALEKLGVEVVVNESMVY"
     gene            complement(1174..2229)
                     /locus_tag="ACZPFK_04095"
     CDS             complement(1174..2229)
                     /locus_tag="ACZPFK_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405524.1"
                     /translation="MKQLGKRAVGLLRDRDFTRMLAVTVVIFISMALLRPALFLKSNN
                     FISMGYQIPELGFYSLAMMMVMVTGGRDLSIVAIGNLACVVSAYIMHYAFERGYTGGA
                     AVPYILAGILAAVAVGVVCGVINGVIVANFNISAMLVTMATSSIFTGLGIILTEGKTV
                     SKTPAEFTYFGNHTLLGLPYTLWLLILALLFSAWLLKCTKFGFEVRFVGSNPRASHYT
                     GMNNRKVLVKAYIYSGVMSAICGLEILARTDTAKSDYALTYVFQAILCAVLGATNPNG
                     GFVKISCMVLSLISLQCLSSGFNMLRLGGYFKEFAWGLLLLVVLSINFFSEEIRRRRA
                     LDRVGRASKEKQKAAGG"
     gene            complement(2226..3239)
                     /locus_tag="ACZPFK_04100"
     CDS             complement(2226..3239)
                     /locus_tag="ACZPFK_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405525.1"
                     /translation="MRAALKKTFRTSQFALLVLLAGTALLFGIVNPQILTLANLYALI
                     RASLVPAVFALSIMLVMVQGGIDMSFGMIGAFSSYVVLFILTENGIFEVPLIAVFLFA
                     VVITVALQLFNWVMVDKLGLQPFITTLGTQTMLKGLLLAFISTDYIYTYPASVAAFGT
                     TYIHSATYESGTSSQLHVSVLIVAALYVLVHLLLQHTNVGRQMYAVGGDVDAARRAGI
                     NVSRVRFLVFLLAGVICGVGGVMHDAIARASMPIPSDLVGKELQSIAAVVLGMGASKQ
                     ARGSVPGTLLGVLLLQFITGNLIMLGIPSYYQQAVSGVIIFVGLIAQMRRRGGKPGKG
                     ELK"
     gene            complement(3236..4759)
                     /locus_tag="ACZPFK_04105"
     CDS             complement(3236..4759)
                     /locus_tag="ACZPFK_04105"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405526.1"
                     /translation="MKNLVSIREMYKSFFGVVALDGMNFSMDEGEIRCLIGENGCGKS
                     TMIKVISGFYTYDRGELEIGGRPYRRITPAESFREGIQVIYQDFSLFPNMTIAENIMM
                     YSTVAEGKPLINAKKTRERAVETLQKIHFDIDPDLYVSELNVAQKQMVAICRAIVQDA
                     KLLVMDEPTTALTTREVEKLFEVVRKLKSHGVAVLFVSHKLDEVLEICESITVMRNGK
                     NVYDSELNGQMLSKEELIYYMTGKRFDNARYEYRPVDAEPLMEVRGYTLPGKFEDVSF
                     SLHRGEIMGVTGLLGCGRGELAEALFGLRAARGGTVLIDGREVGAFKRVDDALAHGIA
                     YVPEDRLTEGLHLDQSIAENSVSRVVGDFAGLLGALDLRGLARQKEKGLSSMYVAGMK
                     PENPAKSLSGGNQQKIVLIKWLASNPRILILNCPTVGVDVGAKSDIHETVRRLAADKG
                     VGVIVISDDILEIMQLCNRVIIMKNGRFDYETPIADTTMEQLEARLAQEEAERGAKA"
     gene            complement(4907..>5122)
                     /locus_tag="ACZPFK_04110"
     CDS             complement(4907..>5122)
                     /locus_tag="ACZPFK_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1405527.1"
                     /translation="NGTIKTICFWDPADAGEAMCSLATKVLGGEEIKDGIDLGVPGFE
                     SCKLEGNTLFGSAWVDVTTENMADYNF"
BASE COUNT          956 a         1656 c         1518 g          992 t
ORIGIN      
        1 tgaatttcag tggccggcaa gaccttttcc gggatgcccc ccagccgctc gatgcggatg
       61 aagccgtcct cctcgatgta ccgcaccaca aagcccagcg agtccatgtg cgcgaacacc
      121 atcaccaccg gcgccgacgg gtcggtcccc tctatccttg ccatgcagtt gcccagggcg
      181 tcggtctgta ccggcagccc gcaggcctcg aactcccgcc gcagataggc ggccagcttt
      241 tcctcatgcc ccgccagggc ggggatcagg gtcgttgctt ccagatactt ttcaatgcgt
      301 tccaacatga cgattgcctc ctttgagctt gtgtcgggcc ccggcggtcg ccggggctca
      361 ataaaccatc gattcgttga cgacgacctc gacccccaat ttttcgaggg cctctttctc
      421 ctttctcggg atgcggtcgg tgaccagcac gtccaggtcg ctcgcgtcgc agacatgggc
      481 aaacacctgc ttgccgtgtt tggagccgtc cgcgacggcg atcacctgct cagaggcctg
      541 gatcatggct tttttcaccc ccacctccag cagggtgcgg ttggagaccc ccttgcgggg
      601 atcgaccgcg tcgcagccga gaaacatctt gttggccttg atgccgcgca gaaactccac
      661 cgtctccatc ccgcacagcg tgtagaccat gggctccacc gtgccgccgg tcatgatcag
      721 ggtcacgctg ccgctggggg ccagcgtgac gccgatcttc aggtcattgg tgatgacggt
      781 gatggctttg tttttgatca gctttgcgat ttccagtgtg gtggacccgg cgtccaggat
      841 gaccacatcg ttctccttga ccagatcggc ggcctgccgg gcgatggccc gcttggcgtc
      901 gatgttgtgg cgcgacttgc tctgagaggg gatctccagg ttcagcctgt cgctgatcgc
      961 catggcgccg ccgtgggcct tgatgatcat ccccttttca tccaggtcgt tcaaatccgc
     1021 gcggatggtg accatcgagg tgttgaagat gcggctgagc tctttggtgc gcaccttttt
     1081 attggtgttg atgtattcca ggatgcgctc ctgccgctcg gcctggaaca tgatctttcc
     1141 gttttccatt ttcggccctc ccgcctgcgc gcctcagccc ccggctgcct tttgcttttc
     1201 tttcgacgcg cggccgaccc ggtccagcgc ccggcggcgg cggatctcct cagagaagaa
     1261 attgatgctc agcacgacca gcagcagcag cccccaggcg aactctttga aatagccgcc
     1321 cagccgcagc atgttgaacc cgctggaaag gcattgcagc gagatcagcg agagcaccat
     1381 gcaggagatc ttgacaaaac cgccgttggg gttggttgcg cccagcaccg cgcagaggat
     1441 ggcctggaac acataggtga gggcgtaatc ggatttggcg gtgtcggtgc gagccaaaat
     1501 ttccaggccg cagatggccg acatgacgcc gctgtagata tacgccttga cgagcacctt
     1561 gcggttgttc atgcccgtgt aatggctggc gcgggggttg gagcccacaa agcgcacctc
     1621 aaagccgaac ttggtgcatt tgagcaacca ggcggaaaag agcagcgcca ggatgagcag
     1681 ccagagcgtg tagggcagcc ccagcagggt gtggttgccg aaataggtga actccgccgg
     1741 ggtcttggac acggtcttgc cctcggtgag gatgatgccc aggcccgtga agatcgacga
     1801 ggtggccatc gtcaccagca tggccgagat gttgaagttc gccacgatga cgccgttgat
     1861 cacaccgcac accaccccca ctgcgacggc cgcaagaata cccgccagaa tatagggcac
     1921 ggcggcgcct ccggtgtagc cgcgctcaaa ggcgtagtgc atgatatagg cgctgaccac
     1981 gcaggccagg ttgccgatgg ccacgatgga caggtcgcgc ccgcccgtga ccatgaccat
     2041 catcatggcc agcgaataaa agcccagctc cgggatctga taccccatgg agataaagtt
     2101 gttcgacttg aggaacaggg ccgggcgcag cagcgccata gagataaaga tgacgacggt
     2161 gaccgccagc atgcgggtga agtccctgtc ccgcagcagg ccaaccgccc ttttgcccag
     2221 ctgtttcatt tcagctcccc tttccccggt tttccgccgc gtcggcgcat ctgtgcaatg
     2281 agcccgacaa agatgatcac gcccgagacc gcctgttggt aataggaggg gatgcccagc
     2341 atgatcaggt tgccggtgat gaactggagc agcaataccc ccagcagggt gcccggcacc
     2401 gagccgcgcg cctgcttgga cgcccccatg cccagcacga cggcggcgat ggactgcagc
     2461 tccttgccca ccaggtcgga cgggatgggc atgctggccc gcgcgatcgc gtcgtgcatc
     2521 acgccgccga cgccgcagat gacgcctgcc agcaggaaca ccagaaaccg cacccgggag
     2581 acgttgatac ccgcccgccg ggctgcgtcc acgtcgccgc ccaccgcata catctgccgg
     2641 cccacgttgg tgtgctgcag cagcaggtgc accagcacat acagcgcggc cacgatgagc
     2701 acgctgacat gcagctggga ggaggtgccg ctctcatagg tggcggaatg gatataggtg
     2761 gtgccgaaag ccgccacgct ggcggggtag gtgtagatat agtcggtgct gatgaaagcc
     2821 agcagcaggc ccttgagcat cgtctgggtg cccagggtgg tgatgaacgg ctgcagcccc
     2881 agcttgtcca ccatcaccca gttgaacagc tgcagcgcca ccgtgatgac cacggcaaag
     2941 aggaacaccg cgatcagcgg cacctcaaag atgccgttct ccgtgaggat gaagagcacc
     3001 acataggagg aaaaggcgcc gatcatgccg aacgacatgt cgatgccgcc ctgcaccatc
     3061 accagcatga tcgacagcgc gaacaccgcc ggcaccagcg aggcccggat cagggcgtac
     3121 aggtttgcaa gggtcaggat ctgggggttg acgatgccga agagcagcgc cgtgcccgcc
     3181 agcagcacca gcagggcgaa ctggctggtg cgaaacgttt ttttcagtgc cgccctcatg
     3241 ctttcgcccc cctctccgct tcctcctgcg cgagccgcgc ctccagctgc tccatcgtgg
     3301 tgtccgcgat gggcgtctca tagtcgaacc gtccgttttt cataatgatc acccggttgc
     3361 acagctgcat gatctcgagg atatcgtcgg agatgacgat cacgcccacc cccttgtcgg
     3421 cggccaggcg gcgcaccgtc tcgtggatgt cgctctttgc gcccacatcc acccccaccg
     3481 tggggcagtt gaggatcagg atgcgcgggt tggacgccag ccatttgatg agcacgatct
     3541 tctgctggtt gccgccggag agggattttg cggggttttc gggcttcatg cccgccacat
     3601 acatggagga gaggcccttt tctttctgcc gggccaggcc gcgcaggtcc agcgcgccca
     3661 gcagcccggc aaaatcgccc accacccgtg agaccgagtt ttcggcaatg ctctggtcca
     3721 ggtgcaggcc ctcggtgagc cggtcctccg gcacataggc gatgccatgg gccagcgcgt
     3781 cgtccacccg tttgaacgcc cccacctcgc ggccatcgat gagtaccgtg ccgccccggg
     3841 ccgcgcgcag gccgaacaga gcctcggcca gctcgccgcg gccacagccc agcagcccgg
     3901 tgacgcccat gatctcaccc cggtgcagcg aaaagctcac gtcctcaaat ttgccaggca
     3961 gcgtgtagcc ccgcacctcc atgagcggct ctgcgtccac cggacggtat tcatacctgg
     4021 cgttgtcaaa gcgcttgccc gtcatataat agatgagctc ttcttttgac agcatctggc
     4081 cgttcagctc ggaatcgtag acgtttttgc cgttgcgcat gacggtgatg ctctcgcaga
     4141 tctccagcac ctcgtccagc ttgtggctga caaaaaggac tgccacgcca tggcttttca
     4201 gcttgcgcac cacctcgaac agcttttcca cctcccgggt ggtgagcgcc gtggtcggct
     4261 cgtccatgac cagcagcttg gcgtcctgca cgatggcgcg gcagatggcc accatctgct
     4321 tttgcgccac gttcagctct gatacataga ggtccgggtc gatgtcgaaa tggatcttct
     4381 gtagggtctc caccgcccgc tcgcgggtct ttttggcgtt gatcagcggc tttccctcgg
     4441 ccacggtgga gtacatcatg atgttctccg caatggtcat gttggggaaa agcgaaaaat
     4501 cctgatagat gacctggatg ccctcccgga acgactcggc cggcgtgatc ctgcgatagg
     4561 gcctgccgcc gatctccagc tcccccctgt cgtaggtata aaaccctgag atgaccttga
     4621 tcatcgtcga tttgccgcag ccgttctcac cgatgaggca gcggatctcc ccctcgtcca
     4681 tggagaagtt catcccgtcc agcgccacca cgccgaaaaa cgacttgtac atttcccgga
     4741 tggaaacgag atttttcaaa gtatcaccat ccctgttatt cgatccccgc ccccaggggc
     4801 gggagcgccg tctggcgaaa aataagctac gggacgcgag gcggcaaacc ccgtgtcccg
     4861 cagccgaaac gtcggtcccc tgcgaggttc tgccggaagc cgggcgctag aagttgtaat
     4921 ccgccatatt ctccgtggtc acatccaccc acgccgagcc gaacagcgtg ttcccctcca
     4981 gcttgcagct ctcaaagccg gggacgccca ggtcgatccc gtccttgatc tcctcgccgc
     5041 ccaggacctt ggtcgccagc gagcacatgg cctcgcccgc gtccgccggg tcccagaaac
     5101 agatggtctt gatggtgccg tt
//
LOCUS       DBMDYA010000117         5116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4014_length_5116_cov_1.736989, whole genome shotgun sequence.
ACCESSION   DBMDYA010000117 DBMDYA010000000
VERSION     DBMDYA010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4014_length_5116_cov_1.736989"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="ACZPFK_04115"
     CDS             complement(<1..1036)
                     /locus_tag="ACZPFK_04115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1405528.1"
                     /translation="MALMTQVKTLRFLREVLLGHGTRVLPYEPDRKPDPPGGGPAQRL
                     PRSGPEAEGMSSAALAGLYRDLAAAKGACVHGLIVARHGRVVSEGWFAPFRPGVWHVT
                     HSLCKSFTGTAVGLAVEEGFFGLDDKVSEYFPEYCTLLTGRRTRAITVRHLLTMCSGI
                     TFNEMGEALERDWVRAIFESEIAFEPGTKFVYNSMNSYLLSALVKKTTGQGLVEFLTP
                     RIFEPLGFGPVGWEKCPCSIEKGGWGMYLDPEDMLKFGQLYLQGGVWRGPQGERRLIP
                     ESWAREAVRTQITGADGEYGWQIWTREEDGSFLMNGMFGQYVAVFPRQDIVAVLTSGN
                     GNLLTDSVSYT"
     gene            complement(1140..1604)
                     /locus_tag="ACZPFK_04120"
     CDS             complement(1140..1604)
                     /locus_tag="ACZPFK_04120"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR1405529.1"
                     /translation="MIKILFICHGNICRSPMAEFLFRDLLQKRGLEGRALVASAATSA
                     EEIGNPVYPPARRKLAQLGIDAGGKRAVQLTRADYGRWDYLIGMEQYNLRNMLRILGG
                     DPQGKVLRLLDLTGRPRDIEDPWYTGDFDTACREIGEGCAALLDRLQREGRL"
     gene            complement(1783..2514)
                     /locus_tag="ACZPFK_04125"
     CDS             complement(1783..2514)
                     /locus_tag="ACZPFK_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405530.1"
                     /translation="MKIIINGDEFTKNELDRWKRKRVEKVLKNLKVTLPIGKNTEELC
                     DKLTLIKLKMSYEEITSLMKLKLIIGQIGMKAAAILSGDKRRTAITTIFADGITAEKF
                     GLIIDSLMLEDSLEYRKINLAACPDHYVLRPFNETLEVIETTGNTPVPTQFFITFKDE
                     TGLKEPRNLNYPYQSTGIAKLKDGTIVGGVRHQFRDTQSGLEVRALVEFPNLCPKIII
                     KEHQKHLAAEWSSWIDWAIKNQSQF"
     gene            complement(2498..3085)
                     /locus_tag="ACZPFK_04130"
     CDS             complement(2498..3085)
                     /locus_tag="ACZPFK_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1405531.1"
                     /translation="MTRTVKKPDIRRQELLDIGVKLFFEAGEKEISIQEVVKQANVAT
                     GLFYYYFKSKNDFIDEALNNYVNNEISSLEMILEDRTLTADKKLDAVLEAYFIYAKKM
                     APLRSSAPFHTERHYALTEKLITQLKSKVNTLVLQGVSENVFEVDDVYTTVGFLLNGL
                     TSVFDASTDISEDSFWVIKKLVYKILNGDQNENNH"
     gene            3830..4363
                     /locus_tag="ACZPFK_04135"
     CDS             3830..4363
                     /locus_tag="ACZPFK_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR1405532.1"
                     /translation="MEIFHGRPADCSGREPREVRTYDLLDRLGIEYLRTDHAPATTME
                     VCKDIDGVLEVLICKNLFLCNRQQTQFYLLMMPGDKPFKTKDLSAQLGVARLSFAGEG
                     FMEQFLDLHPGSVSVLGLMNDAQGRVRLVIDEEVLRGEYLGCHPCQNTSSIKLRTEDL
                     LRKFLPAVRHEPTVVRL"
     gene            complement(4540..>5116)
                     /locus_tag="ACZPFK_04140"
     CDS             complement(4540..>5116)
                     /locus_tag="ACZPFK_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR1405533.1"
                     /translation="GEATEDSDLDVVVIFDELGPQELARYAALLETMDCREKVCGFVS
                     GKAELQGWDRADLFQFCRDTVPVHGSLDFIRPQPGPQDARRAVLTGACNLYHGCCHNL
                     LHEKDPAVLAGLYKGAAFVLQAKYFCESGVYVRRKEELAPLLQGADREVLDASRALRR
                     RPETAAAQFDSLSQQLLRWSGKAIAAYGGGR"
BASE COUNT         1098 a         1531 c         1248 g         1239 t
ORIGIN      
        1 gggtatagga caccgagtcg gtgagcaggt tgccgttgcc cgaggtgagc accgcgacga
       61 tgtcctgccg cggaaagacc gccacatact ggccgaacat cccgttcatc aaaaagctgc
      121 cgtcctcttc ccgcgtccag atctgccagc cgtactcgcc gtccgcgccg gtgatctggg
      181 tgcgcacggc ctcccgcgcc cagctctccg ggatcagacg gcgctcgccc tgcggcccgc
      241 gccagacgcc gccctgcagg tacagctggc cgaatttgag catgtcctcc gggtccaggt
      301 acatgcccca gccgcccttt tcgatgctgc aggggcattt ttcccatccc accgggccga
      361 agcccagcgg ttcgaagatg cgcggggtca aaaactccac caggccctgt ccggtggtct
      421 ttttcaccag cgcgctgagc aggtagctgt tcatgctgtt gtagacgaac tttgtgcccg
      481 gctcaaaggc gatctccgac tcaaagatgg cgcgcaccca gtcccgctcc agcgcctcgc
      541 ccatctcgtt aaaggtgatg ccgctgcaca tggtcagcag atggcgcacc gtgatggccc
      601 gggtgcggcg gcccgtcagc agcgtgcagt actcggggaa atattccgac accttgtcgt
      661 ccaggccaaa aaagccctcc tcaacggcca gccccaccgc ggtgccggta aagctcttgc
      721 acagcgagtg ggtgacatgc cacaccccgg ggcggaaagg ggcaaaccag ccctcgctga
      781 ccacccggcc gtgccgggcc acgatcagcc cgtgcacgca ggcgcccttt gccgcggcca
      841 agtcccgata aagcccggcc agggcggcgc tggacatgcc ctcggcctcg gggccgctgc
      901 ggggcagccg ctgggccggc ccgccgccgg ggggatccgg ctttcgatcc ggctcatagg
      961 gcagcacgcg ggtgccgtgg cccagcagta cctcccgcaa aaagcgcagc gtcttgacct
     1021 gtgtcatcaa agccattgat ctgtccctcc cgtcggcaaa aaactgatat cccctgtatt
     1081 ataccgcgtc cggcccctgg gagcaaaccg caaaacggac cctttgtcgc atccgccgct
     1141 cagagccgcc cctcccgctg cagtcggtcc aacagggccg cgcagccctc gccgatctcc
     1201 cggcaggcgg tgtcgaaatc accggtgtac caggggtctt cgatgtcccg cgggcggccg
     1261 gtgaggtcga gcagccgcag caccttgccc tgcgggtcgc cgcccaggat acgcagcatg
     1321 ttgcggagat tgtactgctc catgccgatg agataatccc agcggccgta gtcggcccgg
     1381 gtgagctgca cggcccgctt gccgcccgcg tcgatgccca gctgcgccag cttgcgccgc
     1441 gccgggggat acaccgggtt gccgatctcc tcggcgctgg tggccgcgct cgcgaccagc
     1501 gcccgcccct ccaggccccg cttttgcagc aggtcgcgga acaaaaactc cgccatcggc
     1561 gagcggcaga tgttgccgtg gcagataaat agtattttta tcattgattt tatatctctt
     1621 cataagcctt caagccttga tgttactgac ttttgggcgt ttttccaatt tttaatattt
     1681 tcccctgatt tcacgcaaat ctgtctaact ccacgcaaat tcacggacaa atggcgtagt
     1741 aaactggtgt gtttttaaac taatcatcat tagaaatttg aattaaaatt gcgattgatt
     1801 tttaattgcc cagtcaatcc agcttgacca ttcagcggcc aaatgttttt gatgttcctt
     1861 gataattatt ttgggacata gatttggaaa ctctaccaat gcccttactt cgaggcccga
     1921 ttgcgtatct ctgaattgat gccgcacacc accaacgatt gttccgtctt taagtttagc
     1981 aatccctgtt gattggtatg gatagtttaa atttctgggt tctttcaatc ccgtttcatc
     2041 cttaaaagta atgaaaaact gggtaggaac aggtgtattt cctgttgttt ctattacttc
     2101 cagggtttca ttgaaaggtc ttaaaacata atggtcagga caagcagcta aatttatctt
     2161 tcgatattcc agcgaatctt ctaacattag agaatcaata attaatccaa atttttctgc
     2221 tgtaatacca tcagcgaaaa tagtagtaat ggcggttcgg cgtttatctc ccgacaaaat
     2281 ggcggcagct ttcattccta tttgaccaat tattaatttt agcttcatta aagatgtaat
     2341 ttcttcataa gacattttaa gttttattaa tgttaattta tcacacaatt cctcagtatt
     2401 tttccctatt ggcaaagtta cttttagatt tttaagaacc ttttccaccc gttttctttt
     2461 ccaacgatct aattcatttt tagtaaactc atccccatta atgattattt tcattttgat
     2521 ctccgttcaa tattttatat acaagttttt ttattaccca aaaagaatct tcactaatat
     2581 ctgtgcttgc atcaaacaca cttgttaatc catttaacaa aaatcctact gtagtataaa
     2641 cgtcatctac ctcaaacaca ttttctgata caccttgcaa taccagcgtg tttaccttac
     2701 tttttaattg tgtaatcaac ttttctgtca aagcataatg cctttcggta tgaaaaggcg
     2761 cacttgaacg aagtggagcc attttctttg catatataaa ataggcctcc aataccgcat
     2821 ctaatttttt atcagcagta agcgttctat cctccagtat catttccaaa gaagagattt
     2881 cattatttac atagttattc aatgcttcgt caataaaatc attcttagac ttaaagtagt
     2941 aatagaacaa tcctgtagca acatttgcct gttttactac ttcctgaatg ctgatttctt
     3001 tttcccctgc ttcaaaaaac aatttcactc ctatatccaa taattcctgt ctgcgtatat
     3061 ccggtttttt tactgttctt gtcatatcgc gccctccact gaatatagtt caattataca
     3121 ttactgaact atattcagtc aagaaaaatt ttgtactccc tatatcaaaa atcagcgatg
     3181 accgtggcaa tgcgctggaa aaagctttcc tcttccggcg cattcaccgt cacatctctc
     3241 ccctgtacgc catcagcagg ttgtaaatct gcgctgcaaa cacatcccca tccggagaat
     3301 tgattcggag caggatgctc cccactgcat ccagttcctt tcggaacagc tcctcattcc
     3361 tcacccagtt cccaaacttc cgtttttaaa tataaattag tttcgtctgt ttatttaaca
     3421 caaatgaaag tctgcgataa acggcgcaat ccaaatggca ttgctgtcaa ataatatggg
     3481 aatacaaaaa acaacatctt attttgaaaa aacaaaagaa taaagtttta ccctttcaat
     3541 aacagccatt ttttatcttc ctctgtccaa ccgcttaatc tttatttctg cgataattct
     3601 cgctagatta gacatgattt tgctgcctcg ccaggtcaca gtctcccaac ccgcaaagct
     3661 cccttttcta ccctatcctc ggcgccgcct atttgccgga tgcagagtgc aattccccac
     3721 ctgtcccggc cactttgccg cggcgggtgc ggcgtggtat aatgggggct gtcccaccca
     3781 aaaccatcac atccgcccgc ctttgcggcg ggcgtgggga ggctatcata tggagatttt
     3841 tcacgggcgg ccggcggatt gcagcggccg cgagccgcgc gaggtgcgca cctacgatct
     3901 gttggaccgg ctggggatcg agtacctgcg caccgaccac gcccccgcca ccaccatgga
     3961 ggtctgcaag gacatcgacg gggtgctgga ggtgctcatc tgcaaaaatc tgttcctctg
     4021 caaccggcag cagacccagt tctatctgct gatgatgccc ggggacaagc ctttcaagac
     4081 caaggacctc tccgcccagc tgggcgtggc ccgtctcagc tttgcgggag agggctttat
     4141 ggaacaattc ctcgacctgc acccgggcag cgtcagcgtg ctgggcctga tgaacgacgc
     4201 acaggggcgg gtgcggctgg tcatcgacga ggaggttctg cggggcgaat acctgggctg
     4261 ccacccctgc caaaacacca gcagcatcaa gctgcgcacc gaggacttgc tgcgcaaatt
     4321 tttgcccgcc gtgcgccacg agcccacggt ggttcggctc tgaggcccgg cgggctgaaa
     4381 cggcgggcgg catacggctc tcgccccggc atgggccgcg tcccggatgc gttttttgcc
     4441 cgcgcgggct atggccgcgc gggtgcgccg cgccctgtat tccccacccg ctttcgccct
     4501 tgtcggcaag ggcctgcaaa gcggccgtcg gggccggggt cagcggccgc cgccataggc
     4561 tgcgatcgcc ttgccgctcc agcgcagcag ctgctgggag agactgtcaa attgagccgc
     4621 cgcggtctcg ggccggcggc gcagcgcccg cgacgcgtcc aggacctccc ggtccgctcc
     4681 ctgcaggagc ggggcaagct cttcctttcg gcggacatag acgccgctct cgcaaaaata
     4741 ttttgcctgc agcacaaacg cagccccttt gtacaggccc gccagaacgg cggggtcttt
     4801 ttcgtgcagc aggttgtggc agcagccgtg gtagagattg caggccccgg tgagcaccgc
     4861 ccgccgcgcg tcctgcgggc ccggctgcgg gcggataaaa tccaggctgc cgtgcaccgg
     4921 caccgtgtcg cggcagaact gaaagagatc ggcccggtcc cagccctgca gctccgcctt
     4981 gccggacaca aacccgcaga ctttctcccg gcagtccatc gtctccagca gcgcggcgta
     5041 ccgcgccagc tcctgcggac ccagctcgtc aaagatcacc accacgtcca ggtcgctgtc
     5101 ctcggtggcc tccccg
//
LOCUS       DBMDYA010000118         5114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4016_length_5114_cov_1.678037, whole genome shotgun sequence.
ACCESSION   DBMDYA010000118 DBMDYA010000000
VERSION     DBMDYA010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_5114_cov_1.678037"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            179..1366
                     /locus_tag="ACZPFK_04145"
     CDS             179..1366
                     /locus_tag="ACZPFK_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1405534.1"
                     /translation="MTEFLIRHFVREPQDARTPAARGRYGLLAGVVGLLCNLLLCGGK
                     FAVGLAFGSVSITADAVNNLSDASSCLITLLGFKLGGKPADREHPFGHARIEYLSGLS
                     VAVLIMAIGVDLLRTSFERVFHPAPVAFSLLSVAVLAVSILVKLWMARFYRAIGRRIG
                     STALEATGMDSRNDVLTTAAVLAAALLARVTGLDLDGWMGLAVAIFILWGGVGIVRET
                     LNPLLGEAPSRELVDLLDQKIMSYDGVLGLHDLMVHDYGPGRRFASVHVEMSASENVM
                     HCHDVIDNIERAVLEEQGIHLVIHLDPISDDDGEVCRHRELAAAKVREIDPRLTIHDF
                     RMVKGNDHTNLIFDVVVPQGFGMKASRLKEVIAQALRRDDPNHFAVVTVDYSYASAPP
                     EKG"
     gene            1503..2696
                     /locus_tag="ACZPFK_04150"
     CDS             1503..2696
                     /locus_tag="ACZPFK_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR1405535.1"
                     /translation="MYEFSNRALASKPSAIREIIKNMNDPTMISFAGGNPAVECFPIP
                     EIKKYSDKILSEYPVKMLLYSTTEGVPSLRQSALAFANRYGRICTERDDIAILTGSQQ
                     AMDFTARMFCNEGDTVVAEDPSFVGALNAFRGSGAKTVGVPMESDGVDLARLEEAFRA
                     TPRPRFFYIIPNFQNPTGLVTSWEKRKAIYALAEKYGVLILEDNPYGELRFEGEYIPP
                     IKSLDQKGLVLYCGSFSKILAPGMRLAYIIADKALVEKLVTIKQCSDTHTNTWSQMVC
                     DEWLRHSDTEAHIAAIRKVYERKCRLMLAEMDQQFDPRIRYTRPSGGMFLWATLPDGV
                     DMMSFVLEAQRRKVAVVPGSAFLCDEDGATQCFRLNYSTPSDEDIVKGIGILGDLTRE
                     MCAGL"
     gene            2878..3267
                     /locus_tag="ACZPFK_04155"
     CDS             2878..3267
                     /locus_tag="ACZPFK_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1405536.1"
                     /translation="MEKRNTVQKQLVLQAVRELQCHPTADEVYQHVAARHPSISKATV
                     YRNLNSLAEDGLLRRVAMPDAADRFDFTVGPHYHIKCACCGRVIDAPAAYMPQLDAEA
                     EQGTGFRISRHDLVFEGLCPACREKRA"
     gene            3344..3886
                     /gene="rbr"
                     /locus_tag="ACZPFK_04160"
     CDS             3344..3886
                     /gene="rbr"
                     /locus_tag="ACZPFK_04160"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1405537.1"
                     /translation="MAELKGSRTEANLMAAFAGESQARNKYTFYASKAKKEGYKVIGD
                     IFEETAGNEKEHAELWFKALHGGEIPDTMTNLADAAAGENYEWTEMYAEFAKVAKEEG
                     FERLAWQFDAVAKIEKTHEERYNDLAKLLKEDKIFAREEEQVWICTNCGHIHVGKSAP
                     AKCPVCDHPQSYFELRVKPY"
     gene            4738..>5114
                     /locus_tag="ACZPFK_04165"
     CDS             4738..>5114
                     /locus_tag="ACZPFK_04165"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR1405538.1"
                     /translation="MLDLLLDTLIDAAKMLPFLFVACLLVEVAEHRQNSRLMKLLSGE
                     GKVGFLAGAVLGTVPQCGFSAMAANLYAGRVITLGTLLSVFIATSDEAFLVLLASPRA
                     LPALGALIAVKFALAAGAGFAVD"
BASE COUNT          982 a         1563 c         1653 g          916 t
ORIGIN      
        1 tctcagcagg ggcgcgcccc ctggcgggaa gaggcttttc ctgagagccg ggcggcggca
       61 ccctttttcg gcttttccgg gccgcgcaaa aaatatttga tttttgcgcg gcgagcggct
      121 ataatttatg cttaataccg catccgtgcg gctttttgag gggaaagggg ggcgtcgctt
      181 gacagagttt ctgatccggc attttgtgcg ggagccgcag gacgcccgca ccccggcggc
      241 gcgcggacgc tacggcctgc tggccggcgt ggtggggctg ctctgcaacc tgctgctctg
      301 cggcggcaag tttgccgtgg gcctggcttt cggcagcgtc tccatcaccg cggatgcagt
      361 gaacaacctg tccgacgcct ccagctgcct catcacgctg ctgggcttca agctgggggg
      421 caagcccgcc gaccgggagc acccctttgg ccacgcccgc atcgagtatc tgtccggcct
      481 gtcggtggcg gtgctcatca tggccatcgg ggtcgacctg ctgcgcacca gctttgagcg
      541 ggtgtttcat cccgcgccgg tggcgttcag cctgctgtcg gtggcggtgc tcgccgtctc
      601 gatcctggtc aagctctgga tggcgcgttt ttaccgcgcc atcggcaggc gcatcggctc
      661 cacagccctg gaggccaccg gcatggacag ccgcaacgac gtgctgacca ccgccgccgt
      721 gctggccgcc gccctgctgg cccgcgttac cggcctcgac ctggacggct ggatgggcct
      781 tgcggtggca atcttcatcc tctggggcgg ggtgggcatc gtgcgggaga ccctgaaccc
      841 tctgctgggg gaggcgccca gcagggagct ggtggatctg ctggaccaga agatcatgag
      901 ctacgacggc gtgctgggtc tgcacgacct gatggtgcac gactacgggc cggggcggcg
      961 gttcgccagc gtccacgtgg agatgagcgc ctctgagaat gtgatgcact gccacgacgt
     1021 gatcgacaat atcgagcgcg cggtgctgga ggagcagggc atccacctgg tgatccacct
     1081 ggaccccatc agcgacgacg acggcgaggt ctgccgccac cgcgagctgg ccgccgccaa
     1141 ggtgcgggag atcgaccccc ggctgaccat ccacgatttt cgcatggtga aaggcaatga
     1201 ccacaccaac ctgatttttg acgtggtggt gccccagggc ttcggcatga aagcctcccg
     1261 cctgaaagag gtgatcgccc aggcgctgcg ccgggacgac cccaaccact tcgccgtggt
     1321 gacggtggat tacagctacg ccagcgcccc gccggaaaag ggctgagcgc gcgggcccac
     1381 aaaggtgcaa aaacgccccg cgccggcccg ctgccggcgc cttgggcggc tttgataaaa
     1441 atgcagtgag acacacggac ggcacagcat gggccgccgc agacagagag gggaaaacgc
     1501 gtatgtatga attttccaac cgggccctgg cctccaagcc ctcggccatc cgcgagatca
     1561 tcaaaaacat gaacgatccc accatgatct cctttgcggg cggcaatccg gcggtggagt
     1621 gctttcccat tccagagatc aaaaaatatt ccgacaagat cctcagcgag tatccggtga
     1681 agatgctgct ctactccacc accgaggggg tgccctcgct gcggcagtcg gcgctggcct
     1741 ttgccaaccg ctacggtcgc atctgcaccg agcgggacga tatcgccatc ctcaccggca
     1801 gccagcaagc catggatttc accgcccgca tgttctgcaa cgagggggat acggtggtgg
     1861 ctgaggaccc cagctttgtg ggggcgctga acgctttccg cggcagcggc gcaaagacgg
     1921 tgggggtgcc catggagagc gacggtgtgg acctcgccag gctggaggag gctttccgcg
     1981 ccacgccccg tcccaggttt ttttacatca tccccaactt tcaaaacccc accggccttg
     2041 tcaccagctg ggagaagcgc aaggccatct acgccctggc ggagaagtac ggcgtgctga
     2101 ttttggagga caacccctac ggcgagctgc gctttgaggg ggagtacatc ccccccatca
     2161 agagcctgga tcaaaagggc ctggtgctgt actgcggcag cttttccaag atattggccc
     2221 ccggcatgcg gctggcctat atcatcgccg acaaggcgct ggtggaaaag ctggtcacca
     2281 tcaaacagtg cagcgacacc cacaccaaca cctggagcca gatggtctgc gacgagtggc
     2341 tgcgccacag cgacaccgag gcgcatatcg ccgcgatccg caaggtatac gagcgcaagt
     2401 gccgcctgat gctggccgag atggaccagc agttcgaccc gcgcatccgc tacacccgcc
     2461 ccagcggcgg tatgttcctg tgggccaccc tgccggacgg cgtggatatg atgtctttcg
     2521 tgctggaggc ccagcgccgc aaggtcgccg tggtgccggg cagcgcgttc ctctgcgacg
     2581 aggacggcgc cacccagtgc ttccggctca actactcgac ccccagcgat gaggatatcg
     2641 tcaagggcat cggcattttg ggcgacctca cccgggagat gtgcgccggg ctgtgacggg
     2701 cgcgccccgc ccggcgcttt ttttataaaa tttgtgaaaa gaacaaaaag tctcagcggc
     2761 gtccgtgcac tgctgagact ttttgatatt aagagaaatt ttgccaaaat tcccttgact
     2821 tttcacagaa accgaatata ctattaaata gtaaaagtta tcaataagga gccggcgatg
     2881 gaaaaacgca ataccgtgca aaagcagctg gtgctgcagg cggtgcggga gctgcaatgt
     2941 caccccacgg ccgacgaggt ctatcagcac gtcgcggcac ggcaccccag tatcagcaag
     3001 gccacggtct accgcaacct caattctctg gcggaggacg ggctgctgcg gcgggtcgcc
     3061 atgccggacg cggccgatcg ttttgatttt acggtggggc cgcactatca catcaaatgc
     3121 gcctgctgcg gccgggtgat cgacgccccc gccgcctata tgcctcagct cgacgccgag
     3181 gcggaacagg gcaccgggtt tcgcatctcc cggcacgacc tggtgtttga gggcctttgc
     3241 cccgcctgcc gcgaaaagcg ggcctgacag ctcgacttat gtttagaccg ccaacggcgg
     3301 ccgcataata aattgaagat catgaaaaat ggaggtagtt atcatggctg aactgaaagg
     3361 atcgaggacc gaagcaaatc tgatggctgc gtttgccggt gagagccagg cgcgcaacaa
     3421 gtacactttc tacgcctcca aggcgaagaa agagggctac aaggtgatcg gcgatatctt
     3481 tgaagagacc gccggcaacg agaaagagca tgccgagctg tggttcaagg ccctgcacgg
     3541 cggcgagatc cccgacacca tgaccaacct ggctgacgcg gccgcgggcg agaactacga
     3601 gtggaccgag atgtacgccg agttcgccaa ggtcgccaag gaagagggct ttgagcggct
     3661 ggcctggcag ttcgacgccg tggccaagat cgagaagacc catgaggagc gctacaacga
     3721 cctggcaaag ctgctcaaag aggacaagat ctttgcccgc gaggaggagc aggtctggat
     3781 ctgcaccaac tgcggccaca tccatgtggg caaatccgcc cccgcgaagt gcccggtctg
     3841 cgaccatccg cagagctact tcgagctgcg cgtcaagccc tactgagttt tttggaccgc
     3901 aaaaacgtcc cgcttcagcg gggcgttttt ttatggtccc agccagagga ctggccagat
     3961 ggccgaaaaa atagcctacg gctatttttt attgctgcga ccctttcatc ccacaagtgt
     4021 gggaccggga ggtacgcggc acccggcgcc ccggcaagga gagccatgat gtgcgctgtt
     4081 tttagcgggg gtggggggcc ggagcaggtg ctggcaaggg gaaccgccgt ggggacgctt
     4141 tcggcgagct gaggggccga gagctgcgcg gtaaagccgc ccctggggtg tggcgagcgg
     4201 cggacacaga ccgggaggtt gagagcccgg cccggtgatt ccacccctca gtcactgcgc
     4261 agcagttccc ctcaaagagg aggcggaatg tgcgtcattc ccgccaaggt gaaagcgccg
     4321 agggtagaaa cagacgagga aaaccacagt gggaccgtcc ccggcgcggc gcaagactga
     4381 ggggcacgca gcccctgccg ccccggcgag gggaggctgc gcggcaaagc cgcgccgggg
     4441 gtggggcaag gcgcatggag ctcgggagac agggcccccg gaccggtaat ccacccctgt
     4501 tatgcccgtc cctgtccgcc accccatccg cgcccagggc tcaggctcgc gcttttttca
     4561 tccctctgtt ccgcttttgt ccgctccgcc gcgcccgggg ccgtcgcggg cgggcggctg
     4621 agacaggagc aaaaaaagca aatgcgaatc acttgcattt agcaaaaagc ggggtataat
     4681 aaataggcgg acaaaacgcg aatgattcgc atttgagaaa aacgggaggg accgcccttg
     4741 ttggaccttt tgctggatac cctgatcgac gcggccaaga tgctgccttt cttgtttgtg
     4801 gcctgcctgc tggtggaggt ggccgagcac cggcagaaca gccgtttgat gaagctgctc
     4861 tccggcgagg gcaaggtggg ctttttggcc ggggccgtgt tgggcaccgt gccccagtgc
     4921 ggtttttccg ccatggcggc aaacctctat gcgggccggg tcatcacgct gggcaccctg
     4981 ctctcggtgt tcatcgccac ctcggacgag gcgtttttgg tgctgctggc cagcccccgg
     5041 gcgctgcccg ccctgggcgc gctgatcgcg gtcaagtttg cgctggcggc gggcgccggc
     5101 tttgcggtgg attt
//
LOCUS       DBMDYA010000119         5103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4024_length_5103_cov_1.286485, whole genome shotgun sequence.
ACCESSION   DBMDYA010000119 DBMDYA010000000
VERSION     DBMDYA010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_5103_cov_1.286485"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..1173)
                     /locus_tag="ACZPFK_04170"
     CDS             complement(100..1173)
                     /locus_tag="ACZPFK_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1405539.1"
                     /translation="MEELYIVTGATGHLGGTIVRLLRAQGAEVRGLLLPGEQPPQGDG
                     VAYYPGDVRDVASLEPLFSGVGARRVTVFHTAGVVDISADQVTEAMEQVNVQGTEHIL
                     ALCKKYQVARLVHVSSVHAIPEGDPLGVIAEAEDFSAQAVVGGYAKTKAAATRAVLAA
                     AAEGLDAVVVHPSGILGPYAARGNHLVQMIADYLAGRLPACVRGGYDFVDVRDVAQGC
                     LDAARLGRRGQCYILSGRHYMVHQVLSMVRREGGVRRLPVLPLWLARAAAPLLEWWAR
                     RRGRRPLYTRYSLYTLSSNDRFSHDKATAELGYRPRDLRETVHDTVLWLRSHDEQLRA
                     KAARQVKTRKKHRGSMKGLPAAG"
     gene            1326..1913
                     /locus_tag="ACZPFK_04175"
     CDS             1326..1913
                     /locus_tag="ACZPFK_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1405540.1"
                     /translation="MKKLTNDHRTRVTRMLIRRAFMSLLAQKPIQSISVKELCERAGI
                     NRSTFYAHYTDLHDLLQQIEDEMFAEFEKALDTLYHLDTTAPEPVAVTTEVFQCLKDN
                     SDLCTVTLSDYGDKAFALKLLQRGRECCMENYLQYFKGASPRQVEYYYAFVSAGCIGL
                     LRRWLDDGMLLPAGELAQLAENIMMRGLAFLQEEG"
     gene            2115..2504
                     /locus_tag="ACZPFK_04180"
     CDS             2115..2504
                     /locus_tag="ACZPFK_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1405541.1"
                     /translation="MKKLIAHLPDSELAVMQALWRAGRPLTRPEIDVGLAGRKDWTVS
                     TVVALLIRLENRGFVGHEKLGRGYLYRPLVTQEEYLAAESKTLLGALYGGSPKNFIAA
                     LYDGGALSGEDIRELQDYLDRAKRGES"
     gene            2504..4813
                     /locus_tag="ACZPFK_04185"
     CDS             2504..4813
                     /locus_tag="ACZPFK_04185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1405542.1"
                     /translation="MAKLFLAFARTGLITGAVILLFVLSAPLLRKKYTARLRRAVWLL
                     LAVLLLFPFSLFPTVLLPGREAPLQIELPVAAAETVLARQQPGSPAANSGRQPDAAGQ
                     VAALPVNAPVPTSRPAAQPAAVAQPADLSPLLPQKPTPLTPAPLSPLGIASFVWLGGA
                     LMLLLWQLLHYALWRRRLRRWDQPLSPQLAQAVDRAAQVVRLHRPPPAFANNRVRTPM
                     LVGALRPVLLVPPRLEQDPGLPLMLRHELIHALHRDLAWKGLLLAVRALHWYHPAVWL
                     LCREAEQDMEYACDEAVLRGSGPAVRAAYGGALLSVASAGAHAPALATPFSSSGRAMK
                     RRLEALFRQGGKRRGDALFACALLLTGIFGGLVGCGIAGETPALASPMPSVPAQTEAD
                     LSLSFHASTEQGIFQTVYSLEKPYLEDCQLIYTDFTTGATKPLCPDPDCDHCGPDCAS
                     FFYAQIGNFYMGIISDQLVLVYNVHPLQDPALLSSGELFPPTIRVETMALDGSGRRVL
                     ATLRPVEAEPETGYVSTHLSYVLSDGAFLYLFAASPVSNSNRPYALLRVDLSDGTSTL
                     LQGFSTFCAGTYPGHLLFGGFDVAAGELRFDAVPLSGGEPRQVYCGYYGQDSGHLLDG
                     QNLYLTNSAERSLKKFNLTTGEEVLITDQLPPLPDSVISRSYFSDQIVGQKLLLSAPF
                     YNEDGAPTEVKHWAVDVVTGEVQEISFLPPEQSDQASFWPSSILAETEDQLCFSVPNE
                     DDPFSHLVLADKEAFWRGELKLRHLDSFP"
     gene            4911..>5103
                     /locus_tag="ACZPFK_04190"
     CDS             4911..>5103
                     /locus_tag="ACZPFK_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405543.1"
                     /translation="MAFTLPLYHEPDFTQPRLANAPDAVLVPAPRDGIAPANYHSTSM
                     YPEYFKLGGRWLLAGESRMD"
BASE COUNT          846 a         1729 c         1541 g          987 t
ORIGIN      
        1 gacgcgcaac ggctgttttt ttagctcatt tatcgccggg cgcggggtcg gacgcttcgc
       61 tctcgcaaaa tcgctcggct tctgccgcag cccgcgcgct catcccgccg cgggcagccc
      121 tttcatgctt ccgcggtgct tttttcgcgt tttcacctgc cgcgccgctt tggcccgcag
      181 ctgctcgtcg tggctgcgca gccagagcac agtatcgtgg accgtctccc gcagatcccg
      241 cggccgatac cccaattcag ccgtcgcctt gtcgtggctg aagcggtcgt tgctggacag
      301 cgtatagagg gaatagcggg tgtacagcgg ccgcctgccg cgccggcgcg cccaccattc
      361 cagcaggggc gcggccgcgc gggccagcca gagcggcagc accggcagac gccgtacccc
      421 gccctcgcgc cgcaccatgc tcagcacctg atgtaccata taatgccgcc ccgagaggat
      481 gtagcactgg ccccgccgcc ccagccgcgc cgcatcaagg cagccctgcg ccacgtcccg
      541 cacgtccaca aagtcatatc ccccgcgcac gcaggcgggc agccgccccg ccagataatc
      601 tgcgatcatc tgcaccagat ggttgccccg ggcggcgtag gggcccagga tgcccgaggg
      661 atgcaccacc accgcgtcca gcccctcggc ggccgccgcc agcaccgcgc gggtggccgc
      721 cgctttggtt ttggcataac cgccgaccac cgcctgtgct gaaaagtcct ccgcctccgc
      781 gatcactccc agcgggtccc cctccgggat agcgtggacc gagctgacgt ggaccagccg
      841 cgccacctgg tattttttgc acagggccaa aatatgttcc gttccctgca cattgacctg
      901 ctccatggcc tcggtcacct ggtcggcgga aatgtccacc acccccgccg tatggaacac
      961 ggtcacgcgc cgcgccccga cgccgctgaa cagcggctcc aggctcgcca catctcgcac
     1021 gtcccccgga taataagcca cgccgtcccc ttgcgggggc tgctcgccgg gcagaagcaa
     1081 gccccgcacc tcggccccct gcgcgcgcag cagacgcacg atagtgccgc ccaaatggcc
     1141 tgtggcgccg gtcacgatat aaagctcttc catctttttt catctccatt ttccgacacc
     1201 atgttgtttt acttacccct ctgtattata atataaacag tcccaacact gcaacgaaca
     1261 ctgcggccgg caatgccgga caaaccgaca cagaccacag attctgtggg aaagcgaggc
     1321 ctgttttgaa aaagttaaca aacgaccacc gcacgcgggt gacccggatg ctcatccgcc
     1381 gggcttttat gtccctgctg gcccaaaagc cgatccagag catctcggtc aaagagctct
     1441 gcgagcgcgc ggggatcaac cgcagcacct tttatgccca ttataccgac ctgcacgacc
     1501 tgctgcagca gatcgaggac gagatgttcg ccgagtttga aaaggccttg gacacccttt
     1561 atcatctgga cacgaccgcc cccgagccgg tggccgtcac caccgaggtg ttccagtgcc
     1621 tcaaggacaa ctccgatctg tgcaccgtca ccctcagcga ttacggggac aaggcgtttg
     1681 cgctcaagct gctgcagcgc gggcgggagt gctgcatgga aaattatctg caatatttca
     1741 aaggcgcctc gccgcggcag gtggaatatt actacgcctt tgtcagcgcg ggctgcatcg
     1801 ggctgctgcg gcgctggctg gacgacggca tgctgctgcc cgcaggtgag ctggctcagc
     1861 tggcggaaaa tatcatgatg cgcggcctgg cctttttgca agaggagggc tgaagctccg
     1921 tctgcggcgc ttttgctccc gttcccaagg gccaaggccc ggtcagatgg cggcgcctcg
     1981 ccgcctgctt gcccctctcg gccgcacccg ccggccctca aaaccagctt tcgacacgtc
     2041 ttgactttta tctattacaa ttgtaatata aaggcgaaat atgttacatt tgtaagagat
     2101 aagggggagg tcttgtgaaa aagctcatcg cgcacctgcc ggattccgag ctggcggtga
     2161 tgcaggcgct ttggcgtgcg ggcaggccgc tcacccgccc cgagatcgac gtcgggctgg
     2221 ccgggcgcaa ggattggacg gtgagcacgg tggtggcgct gctgatccgc ttggaaaacc
     2281 ggggcttcgt gggccatgaa aagctgggcc gcggctatct ctaccgcccg ctcgtcacac
     2341 aggaggaata cctcgccgcc gagagcaaaa cgctgctggg cgcgctctac ggcggcagcc
     2401 ccaaaaactt catcgccgcg ctgtacgacg gcggcgcgct gagcggcgag gatatccggg
     2461 aattacagga ttatctggac cgggcgaagc gaggtgagag ttgatggcaa agctgtttct
     2521 cgcttttgcc cgcaccgggc tcatcaccgg ggccgtcatt ctgctctttg ttctttcggc
     2581 ccccctgctg cgcaaaaagt ataccgcccg gctgcgccgc gcggtttggc tgctgctggc
     2641 cgtgctgctg ctgttcccct tttcgctctt ccccaccgtg ctgctgcccg gccgtgaggc
     2701 ccccttgcag atcgagctgc ccgtggccgc ggccgagacc gtgctcgcac ggcaacagcc
     2761 cggcagcccg gccgccaaca gcgggcggca gccggacgcc gccggtcagg tcgcggcgct
     2821 ccctgtaaac gcccctgtgc caacgtcgcg gccagcggca cagccggcgg ccgtggcgca
     2881 gccggcggat ctttcgcccc tgctgccgca aaagccgacg cccctcaccc ctgcgccgct
     2941 ctccccgctg gggatcgcgt cttttgtgtg gctcggcggt gcgctgatgc ttttgctgtg
     3001 gcagctgctg cactacgcgc tgtggcggcg gcggttgcgc cgctgggatc agcccctttc
     3061 gcctcagctg gcgcaggcgg tcgaccgggc tgcccaggtg gtgcggctgc accgcccgcc
     3121 gcctgccttt gccaacaacc gcgtccgcac cccgatgctg gtgggcgcgc tgcggcccgt
     3181 gctgctggtg ccgccccggt tggagcagga cccaggcctg cccctgatgc tgcgccacga
     3241 gctgatccac gcgctgcatc gtgatcttgc ctggaaaggg ctgctgctgg cggtgcgggc
     3301 gctgcactgg tatcacccgg cggtctggct gctctgccgc gaggcggaac aagatatgga
     3361 atacgcctgc gacgaggcgg tgctgcgcgg cagcggcccc gccgtccgcg cggcctacgg
     3421 cggcgcgctg ctgtccgtgg cgagcgcggg cgcgcacgcc ccggccctgg ccacgccctt
     3481 ttcttccagc ggcagggcga tgaagcggcg gctggaggcg ctgttccgtc aaggcggcaa
     3541 gcggcgcggc gatgcgcttt ttgcctgtgc gcttcttttg acgggtatct ttggcggtct
     3601 tgtgggctgc ggcatcgcgg gggaaacgcc cgccctggcc tcccccatgc cctccgtacc
     3661 ggcgcagacg gaggccgatt tgtccctttc ttttcacgcc tctaccgagc agggcatttt
     3721 tcaaacggtc tactctttgg agaagccgta cctggaagac tgtcagctga tctacaccga
     3781 tttcaccacc ggggcgacaa aaccgctctg cccggacccg gactgcgacc attgcggccc
     3841 ggactgcgcc tcgttttttt acgctcagat aggcaacttc tacatgggca ttatctccga
     3901 tcagctggtg ctggtctaca atgtccaccc tctgcaggat cccgcgcttc tctcctcggg
     3961 agagcttttt ccacccacga tccgtgtgga gacgatggcg ctggacggct caggccgccg
     4021 cgtgctcgcc acgctgcgcc cggtggaggc ggagccggaa acgggatacg tctcgacgca
     4081 cctctcttac gtcctttcgg acggcgcttt tctctatctg ttcgcagctt ctccggtcag
     4141 caattcaaat aggccctatg cgctgcttcg ggttgatctt tccgacggca cgtcaacgct
     4201 gttgcagggg ttctccacct tctgtgcagg gacctatccg ggtcatctgc tctttggggg
     4261 ctttgatgtg gcagccggcg agctgcgctt tgacgccgtg ccgctgagcg gcggggagcc
     4321 gcgccaggtc tattgcggct attacggaca ggacagcggt catctgctgg atgggcaaaa
     4381 cctctatctc accaactctg ccgaacggag cctcaaaaag tttaatctca ccactgggga
     4441 agaggtgctg atcaccgacc aactgccacc cttgccggac agtgtgattt cgcggagcta
     4501 tttctcagac cagatcgtcg gccaaaagct gctactttct gctccattct acaatgagga
     4561 cggcgcgccc accgaggtca agcactgggc ggtggacgtg gtgaccggcg aggtgcagga
     4621 gatctctttt ctccccccgg agcaaagcga tcaagcctct ttctggcctt cgagtattct
     4681 ggccgagacg gaggaccagc tctgcttctc cgtccccaat gaagatgacc ccttttccca
     4741 tctggtgctg gccgacaagg aggcgttctg gcggggagag ctcaagctgc ggcatctcga
     4801 tagctttccc tgagcgcagg gttcgacatc ctccctgttt tgtgttaaaa ttttcaaata
     4861 aggcgtcccg cacgcagggc gcaaaaaaca caacaacgga gggatgcggc atggcgttca
     4921 ccctgcccct ttatcacgag cccgacttca cccagccccg gctggcaaac gcgccggacg
     4981 cggttttggt ccccgcgccg cgggacggca tcgcgccggc aaactatcat tccaccagca
     5041 tgtacccgga gtacttcaag ctcggcggcc gctggctgct ggcgggggag agccgcatgg
     5101 act
//
LOCUS       DBMDYA010000120         5092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4032_length_5092_cov_1.644560, whole genome shotgun sequence.
ACCESSION   DBMDYA010000120 DBMDYA010000000
VERSION     DBMDYA010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4032_length_5092_cov_1.644560"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /gene="ruvX"
                     /locus_tag="ACZPFK_04195"
     CDS             complement(<1..311)
                     /gene="ruvX"
                     /locus_tag="ACZPFK_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1405544.1"
                     /translation="MKILAVDYGDARTGLAMCDRTEFLASPIGIIEEKSMPKVAEKIV
                     YAAREYEAQMIVIGLPLNMDGSEGPRAEKSRKLASILHTILPLPIELWDERSSTVSAA
                     N"
     gene            complement(963..1601)
                     /locus_tag="ACZPFK_04200"
     CDS             complement(963..1601)
                     /locus_tag="ACZPFK_04200"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405545.1"
                     /translation="MSVLEFEQVSYCYDKGKMILENLQQKFERGKIYAILGPSGCGKT
                     TALSLLGGLDVPTQGKILFEGEDILNRGLEHHRREHVSFVFQNYNLIDYMTPEENVKL
                     TADQEAIPILERVGLSKEECRRNVLKLSGGQQQRVAVARALASENPVILADEPTGNLD
                     EDTAVEIMELFREAAREYNKCVIIVSHSNEVARQADVAFVLKRGKLVLAELS"
     gene            complement(1617..4520)
                     /locus_tag="ACZPFK_04205"
     CDS             complement(1617..4520)
                     /locus_tag="ACZPFK_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405546.1"
                     /translation="MNVLKRAVLYLTRKKARSILLLLLLFFMGLFMLAGLSIRAGAGQ
                     AAEDMRKSISSGLEIKMDAVSGDEIYTTSYNEKGELVRTLKHSLITESVAERLASIPG
                     VSGYYSEMGAEMLYTGLNVVPGGYAEDLKALEGKEEAADLERIACSSAWSKANDFHVV
                     QESEYYPYFRNGAFELIAGRHLDIEDTGKILISEELAARNGLEIGDVIEGQNFDVITG
                     ELYGETYRAEIVGIFRINFEQKLSEWTAEPKILANTVFAPFELRHWGQRQYNAFYGGD
                     QLATEEDRLLGSVTLFVEDPAELDGIEEQIKADAEVDWSYYTIQRYDADYKAAAKPLF
                     TMTLFATCMVVVMIVGTLLILSLVLAMWMRGRKHEIDVLTFLGTSKQMILGQFLLEMG
                     IVVVIAFSISSLFASPVTRAIGNTMTEIVNPSEEADSFSTTYEAATGLTQINRAPVRQ
                     EALPYQISYGVSIGTLSSMALVAFGTVVLAFQKMQNTALLSQKGSDIHHWSFQKAGAK
                     GAMKAHQRAFLYITRKTGKSVLLLFALFVIMGLLLCGISIRLASEHAAAQLRESLGGY
                     FKIAPDYRKSEVVNQVDHELLAYIAGLDEIEAVNAMDLCYMDVRGISLNPGKFSVEQD
                     EKSNMARILGNMDTSLHEYFSLGIFELVEGAPIRETDSGKALISSELAQRNQLKVGDR
                     LTLTTLTEDIRDGALKKTYDLEIVGLFSEKQQASGIASQTPECDMPLNFIFTDISTTQ
                     QMMQDLQQGRKQVYSGGAVFFVKDPKRMEDVILTIEQAGIFDKEFTKLTVNNAAYQNS
                     MEPLSRLSNLSLMMLAVIAGVGVILLTLILTLWERDRIHETGILMSFGVRKRNIWWQR
                     LVECASIFIVSLVVSVGVFLPVSAKMGDWLYGQASASVEQSVGSERPENGMTWEAVRT
                     QSLENDVAFHMELSPGTILLSGLGGLVLVGASMSTAFFSNARHRPKELLASME"
     gene            complement(4820..>5092)
                     /locus_tag="ACZPFK_04210"
     CDS             complement(4820..>5092)
                     /locus_tag="ACZPFK_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR1405547.1"
                     /translation="INATEFGNAFSELNDPIYQKGRFERQVAQRKALDPASKAQVDYD
                     YVTALEYGLPPTGGLGFGVDRLVMLLTDSASIRDVLLFPTMKPVDN"
BASE COUNT         1206 a         1500 c         1097 g         1289 t
ORIGIN      
        1 atgtttgccg cgctcaccgt gctgctgcgc tcgtcccaca gctcgatggg cagcggcagg
       61 atggtgtgca gaatgctcgc cagcttgcgg cttttctccg cccgcgggcc ctccgagccg
      121 tccatgttca gcggcaggcc gatgacgatc atctgcgcct catattcccg ggccgcatag
      181 acgatctttt ccgccacctt gggcatcgat ttttcctcga tgatgccaat gggggaagcg
      241 agaaactcgg tgcggtcgca catggcaagg ccggtgcggg cgtcgccgta atccactgca
      301 agaattttca tggagcattt ctcctttgat ttcatttcgg taaagaacag ccgctggcta
      361 ttttttcggc cagatggccc gtgcccgggc tgcagccatc gagataagtt tttattgcga
      421 agcaatcaaa acagccgcag gctactttag gggtcatccg gcccgtcccc aggccacggc
      481 cgtcaggatg aaaaatatct cgacgccagt ttattatatc aacgccgggc tcccttttca
      541 agcttgccga aaaagcgtcc ccgcccctgg ccgggatcgg cgggcagaat atgggggaca
      601 taaaaaggcc ggcgattaaa gccctcaact tttatctctg aaaaattctt gcaaaaattc
      661 acctgtccat cccgagcccg gcaaaagcgg aagattcccg actctccttg tcaccggctt
      721 tagaatcaga accttcagtt tgccccatgg atcggtcttg gggcgcctgt cccagttttc
      781 tttatcgttc gatcccttgc tttctgattt tacggtctcc cgccagcctt tgcctctagt
      841 ccccaccagc ttgatttttg agcttgattg gcccgcagtt ttgtaaaaag agggcttcca
      901 cggtggattt ctctcaaaat acaaacccag ccccgcaaat ttctatcggg tgctttgcat
      961 tgtcaggata attctgcaag caccagcttc ccccgcttca gcacaaacgc aacgtccgcc
     1021 tgccttgcca cctcattcga atgagagacg ataatcacac atttattgta ttcccttgcc
     1081 gcctcgcgaa acaattccat aatctctacc gcggtgtcct catccaggtt tccggtcggt
     1141 tcgtccgcca gaatcaccgg gttctctgac gccaaagcac gtgcaacggc tacgcgctgc
     1201 tgttgtcccc cggacaactt caaaacattc cgcctgcact cctcttttga cagccccacc
     1261 ctctccagta tcgggatcgc ctcttgatcg gcagtgagtt ttacgttttc ctccggggtc
     1321 atataatcaa tcaaattata gttttgaaaa acaaaagaca cgtgctcacg acggtgatgc
     1381 tccaaccctc tgttcaaaat atcttcgccc tcaaaaagaa tttttccctg tgtgggcacg
     1441 tcaagaccgc ccaaaagcga aagcgccgtg gtttttccgc agccagacgg gcccagaatc
     1501 gcataaatct ttccgcgctc aaatttctgc tgcagatttt ctaaaatcat ttttccttta
     1561 tcgtagcaat aggacacttg ctcaaattcc aatacactca tgcgcttctc ctatctttat
     1621 tccatggatg caagcagttc ttttggtctg tgacgggcgt tggaaaaaaa cgccgtgctc
     1681 atagaagctc caactaaaac aagccctccc aggcccgaaa gcaagatcgt cccaggcgac
     1741 agttccatat ggaacgcaac atcgttttca agggattgtg tgcgtaccgc ctcccacgtc
     1801 attccattct ctggcctttc actccccacc gactgctcca cgcttgccga agcctgccca
     1861 taaagccaat cgcccatttt tgctgaaacc ggcagaaata ctccgacgga aacaacaagc
     1921 gacacaataa atatggaggc gcattccacc agcctctgcc accaaatatt gcgcttacga
     1981 acaccaaaag acatcaatat ccccgtttca tggatacggt cgcgctccca cagggtgaga
     2041 atcagggtaa ggagaatcac gccaacccct gcaataaccg ccagcatcat cagcgacaga
     2101 ttgcttaacc ggctgagcgg ctccatgctg ttttgatatg ccgcattgtt caccgtaagt
     2161 tttgtaaact ccttgtcaaa aatccccgct tgctctattg tgagaatgac gtcttccatt
     2221 cttttcggat ctttgacaaa aaaaaccgcg ccgcctgaat agacctgctt tctcccctgc
     2281 tgcagatcct gcatcatctg ctgggttgta gaaatgtcgg taaaaataaa gttaagcggc
     2341 atatcacatt ccggcgtttg cgacgctata cctgaagctt gctgcttttc tgaaaacaat
     2401 cccacaattt cgagatcata tgtttttttc aaagctccat ctcttatatc ctccgtcaat
     2461 gtggttagcg taagacggtc tcccactttc aattggtttc tctgtgcaag ctcgctggat
     2521 atcagggctt tcccggaatc tgtctctcta ataggcgccc cctcgacaag ctcaaaaatt
     2581 ccaagagaaa aatactcgtg caaactggta tccatattcc ccaatatcct tgccatattg
     2641 gatttttcat cctgctcaac cgagaatttg ccgggattca acgagatgcc ccgaacatcc
     2701 atgtagcata gatccatcgc atttacagct tcaatttcat caagcccggc gatgtaggcc
     2761 agcagctcgt gatccacctg attcactact tcactttttc gataatcagg ggcaatctta
     2821 aaatatcccc ccagactctc tctgagctgt gccgccgcat gctcggacgc caaacggatg
     2881 gagatcccgc ataagagcag ccccattatg acaaagagcg caaacagtaa taacacgctt
     2941 ttccccgttt ttctcgtgat atataagaat gcccgctgat gcgctttcat cgcgccctta
     3001 gcgccagctt tttgaaaact ccagtgatga atatccgagc ctttttgcga aagcagagcg
     3061 gtgttctgca ttttttgaaa tgctaaaacg accgtgccaa aggcgacaag cgccatggac
     3121 gacaaggtcc cgatggatac cccgtaagag atctgatagg gaagcgcttc ctgtctaacc
     3181 ggcgccctgt tgatctgagt aaggcccgta gccgcttcat aggtcgtgct aaaagaatca
     3241 gcttcctctg atggatttac aatttccgtc atcgtgttcc caatagcgcg cgtcacaggg
     3301 gaagcaaaca gggacgatat cgaaaacgca attaccacaa caatccccat ctccagaaga
     3361 aactggccca aaatcatttg cttgctcgtg ccgaggaaag tcaaaacatc aatttcatgc
     3421 tttctgcccc gcatccacat ggcaagcacc aaggagagga tcagcagtgt tccgacaatc
     3481 ataaccacca ccatgcaggt ggcaaacagc gtcatggtaa aaagcggttt tgcagcggct
     3541 ttgtaatccg cgtcatagcg ctgaatggta taataactcc aatccacctc tgcatccgct
     3601 ttgatttgct cctctatccc gtccagttcg gcgggatcct ctacaaagag ggtaacgctg
     3661 cctaagagcc tatcctcctc tgtcgcaagt tgatcccctc cgtaaaaagc attgtattgg
     3721 cgctgccccc aatgccgaag ctcaaagggt gcaaaaaccg tattggcgag aatcttgggt
     3781 tcggcagtcc attcagaaag cttctgttca aaattgatgc ggaagatccc cacgatttcc
     3841 gcgcgatacg tctcaccgta gagctccccc gttataacgt caaaattttg tccctctatg
     3901 acatccccta tctccagccc gtttctcgcg gcaagctctt ctgaaataag aatttttccc
     3961 gtgtcctcta tatccagatg gcggcccgcg atcagctcaa acgcaccgtt tctaaaatag
     4021 gggtagtatt cgctttcctg caccacatga aaatcattcg ctttgctcca tgcactggaa
     4081 caggcaattc tttcgagatc cgccgcctcc tcctttccct ccagcgcttt caggtcttcc
     4141 gcataacctc ccggcactac attcagccct gtatagagca tttcagcgcc catctcgctg
     4201 taataaccgc tgacgccggg gatcgacgcc agcctctccg cgacagactc cgtaatcagg
     4261 gaatgcttta aggtgcgcac cagttccccc ttttcattat agctggtcgt atagatctca
     4321 tcccctgaaa ccgcatccat tttaatctcc aagccagagg agatactctt gcgcatgtcc
     4381 tctgccgctt gccccgcacc agcgcggatc gacaaacccg ccagcataaa aagccccata
     4441 aaaaacagaa gcagaagcag cagtatactg cgcgcttttt tccttgtgag ataaagaaca
     4501 gctcgcttca acacattcat tgtaggctcc tcccgcgcac attccccttt attctcccgg
     4561 tcgcttaggc agattatagc atggggcaag ctattttttc tatcctcttt tttatccaaa
     4621 aaaagacagg cgctccacaa actccgtgaa atgcctgtcc tgttctttta cttctttcag
     4681 aagattgccc tttcagccgc gcctaaaatc ttctctgctg aaattttcgc atggctcggc
     4741 ttaaagcctt gatctccgct ccacttttgc cattaaattg tagagcgttt cattttcaat
     4801 ttcttgatat atcacacatt taattgtcga ccggcttcat cgtcgggaag agcagcacgt
     4861 cgcggatgga ggccgaatcg gtgagcagca tcaccaaccg gtccacgccg aagcccaggc
     4921 cgcccgtggg cggcaggccg tactccagcg ccgtgacgta atcgtagtcc acctgcgcct
     4981 tgctggccgg gtccagcgcc ttgcgctggg ccacctgccg ctcgaagcgg cccttttggt
     5041 agatggggtc gttgagctcg ctgaacgcat tgccgaactc ggtggcgttg at
//
LOCUS       DBMDYA010000121         5091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4035_length_5091_cov_1.557715, whole genome shotgun sequence.
ACCESSION   DBMDYA010000121 DBMDYA010000000
VERSION     DBMDYA010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_5091_cov_1.557715"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="ACZPFK_04215"
     CDS             <1..860
                     /locus_tag="ACZPFK_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HIR1405548.1"
                     /translation="ASLEALLHQEVQGQRTYTIQAGDTPWKIAGSNDVTLDDLYALNP
                     ILENGSNMPVGAELVIGQAVPFLQVKAIVTTVEREPISYETETEYSTDLAFGVQRILQ
                     EGVEGEKDVTYETTYVGGQRTERRAVGEPVVITEPVTQIQQMGVYMTSDGNMIKPGNG
                     IMMFPVDYGGYKGMSRGYSGPLLPAHNGLDLRGYVGTPIYAAQSGVVTYAGQTRGGYG
                     IHVMINHGGGVQTLYGHCSGLAVSVGQVVEQGQLIAYVGSTGWSTGPHCHFEVIVNGV
                     RRDPMNYLG"
     gene            1061..2809
                     /locus_tag="ACZPFK_04220"
     CDS             1061..2809
                     /locus_tag="ACZPFK_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1405549.1"
                     /translation="MAQATAARPGTAPPFKSRASVRLWRLKALLGQLFYKVGEAAELD
                     SVRFWRALQRAGRVLARLLWRPLRSLLAAVGRGLENFWTDLTAPFHRAHSGASHIREL
                     VADEQQNGAGHAARLALRYLGSGVRRYAYLGKNLLYYLLPLGAGAVFVFTVVNVLDAT
                     YVLAVENDGQVVGYVRQEAVYEEAARMVQDQIIYTGDEQRWALSPTFTISAARAEEVS
                     DSQTLADAILRSSGEEITEAVGLYVDGQFYGATTEGGQLEAAIAAIKAPYEAENPGAE
                     IGFVKSVELRRGIYLAESVKDYSELQALFDQPVQGQRTYTIQAGDTPLIIAGANDITL
                     DELYALNPVLENGSNMPVGQELIIGESVSFLQVKVVKTQVEQEEIPFETIKTDDDSLD
                     RGKTKTTQQGVKGLQEVTYQYTYIDGRMVDRTQIGDPVVLQEPVEEHISVGTYISQAG
                     YSVTPSGSGGMIFPVGPGFTYMSRGFSGAYAHNGLDLCAAYGTPIYAAQSGVVTYASA
                     TAGGYGIHVIINHGGGVQTLYGHCSALVVGAGQSVARGQVIAYVGSTGNSTGNHCHFE
                     VIINGARVNGAPYVGI"
     gene            3053..4336
                     /locus_tag="ACZPFK_04225"
     CDS             3053..4336
                     /locus_tag="ACZPFK_04225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1405550.1"
                     /translation="MEKFVVRGGTPLVGEVTVSGAKNAAVAILPATILAGGKCVIENL
                     PNISDVATSVEILHAMGASIRMIDSSTLEVDTSRIVVTDVPAEMSRQMRASYYFLGAL
                     LGRFGRGCVSMPGGCDLGTRPIDQHLKAFGSLGASYNVEYGMIRVSSSVLTAEHIFFD
                     TVSVGATINAMLAAVMAEGLTVLENVAKEPHIVDVANFLNTMGADVRGAGTDVIKIRG
                     VREMHGCTYSIIPDQIEAGSYMAAATITRGNVLIRNVIPKHLEPITAKLEKAGATIEE
                     FDDAVRVCCVGDIEPLNIKTMPHPGFPTDMQPLMAVLLSVAKGTSIITEGIWENRFRY
                     VDELIRMGANIQVDGRVAVIEGVPELQPAPVRATDLRAGAALVVAALAAGGVSEIEEI
                     SHIERGYEDLVGKLRGLGADIKRIDIPGERLRQAL"
     gene            4455..>5091
                     /locus_tag="ACZPFK_04230"
     CDS             4455..>5091
                     /locus_tag="ACZPFK_04230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405551.1"
                     /translation="MATFLSLYSGSSGNCALVGENGRYLCVDMGKNCKTTINALYSAG
                     LSVADLDGILITHEHSDHVSGLRVFLKHYPVPVYGSEATLAYLADHGMVPPTAQLQVL
                     DGSGRVGEFEVRSFHTSHDSVDCCGFRITAPSGRTAAMATDLGVITQEVMQGLAGADL
                     VVLEANYDAVRLRTGHYPYYLKARIASPRGHLCNAETGETLTRLLQCGCERF"
BASE COUNT          943 a         1563 c         1720 g          865 t
ORIGIN      
        1 atgcctcgct ggaggcgctg ctgcaccagg aggtgcaggg ccagcgcacc tacaccatcc
       61 aggcggggga tacgccctgg aagatcgcgg gctccaacga cgtcacgctg gatgacctgt
      121 acgccctgaa cccgatcctt gaaaacggca gcaacatgcc ggtgggggcg gagctggtca
      181 tcggccaggc ggtcccgttt ttacaggtca aggccatcgt caccaccgtg gagcgggagc
      241 ccatctctta tgagaccgag accgagtaca gcaccgatct ggcctttggg gtgcagcgca
      301 tcctgcagga gggggtggag ggcgaaaagg acgtcaccta cgagaccacc tatgtgggcg
      361 gccagagaac agagcgcagg gccgtgggcg agccggtggt gatcaccgag cccgtgaccc
      421 agatccagca gatgggcgtg tatatgacca gcgacggcaa catgatcaag cccggcaacg
      481 gcatcatgat gttcccggtg gactacggcg gctacaaggg catgagccgc ggctacagcg
      541 gcccgctgct gccggcccac aacggcctcg acctgcgcgg ctatgtgggc acccccatct
      601 atgcggccca gagcggcgtg gtgacctatg cgggccagac ccggggcggt tacggcatcc
      661 acgtcatgat caaccacggc ggcggcgtgc agacccttta cggccactgc agcggtttgg
      721 cggtcagcgt ggggcaggtg gtggagcagg gccagttgat cgcctatgtg ggctccaccg
      781 gctggtccac tggcccgcac tgccactttg aggtcattgt caacggcgtg cgccgcgacc
      841 ccatgaacta tctgggctga gggctgcggg gcgcgtaaac catgaaagtt ttgtaaaatc
      901 cttgcgggaa agcgcgaaat tgagtataat taaaaactga aaacagcaca gcggcgggcc
      961 caaggcccgg cggcgcgggg aaactcttcc gcgtctccgg gcgcggaagg cccgcgctgt
     1021 cgttaccggc ccggctgcgg ggcggagggg aggcgcaaaa atggcacagg caacagcggc
     1081 aaggccggga acggcccctc cgttcaagag ccgcgcctcg gtgcgccttt ggcggctcaa
     1141 ggccctgttg ggccagctgt tctacaaggt cggcgaggcg gcggagctgg acagcgtccg
     1201 cttttggcgt gccctgcaga gggccggccg agtgctggca cggctgctct ggcggccgct
     1261 gcgcagcctc ctggcggcgg tcggccgggg cctcgaaaac ttttggacgg atctcaccgc
     1321 cccgtttcac cgggcccaca gcggcgcctc ccacatccgc gagctggttg cggacgagca
     1381 gcagaacggg gcggggcacg cggcccggct ggcgctgcgg tacctcggca gcggcgtgcg
     1441 ccgctatgct tatctgggca agaacctgct gtattacctg ctgccgctgg gggccggggc
     1501 ggtcttcgtc ttcacggtcg tcaacgtgct ggacgccacc tatgtgctcg cggtggaaaa
     1561 cgacggccag gtggtgggct atgtgcggca ggaggccgtc tatgaagagg cggcccgcat
     1621 ggtgcaggac cagatcatct acaccggcga cgagcagcgc tgggcgctga gccccacctt
     1681 taccatctcc gccgcgcggg cggaggaggt cagcgacagc cagacgctgg cggacgcgat
     1741 cctgcgcagc tcgggtgagg agatcaccga ggcggtgggg ctgtatgtgg acggccagtt
     1801 ctacggcgca accaccgaag gcgggcagct ggaggcggcc atcgccgcca tcaaagcgcc
     1861 ctatgaggcg gaaaatccgg gcgcagagat cgggtttgtc aaaagcgtcg agctgcgccg
     1921 gggcatctat cttgccgaga gcgtcaagga ttacagcgag ctgcaggctc tttttgacca
     1981 gccggtgcag ggccagcgca cctataccat ccaggcgggg gacaccccgc tcatcatcgc
     2041 gggcgccaac gacatcacgc tggacgagct ctatgctttg aaccccgtgc tggaaaacgg
     2101 cagcaatatg ccggtggggc aggagctcat catcggcgag tcggtgtcct ttttgcaggt
     2161 caaggtggtc aagacgcagg tcgagcagga ggagatcccc tttgagacca tcaagaccga
     2221 cgacgactcg ctggaccggg gcaagaccaa gaccacccag cagggggtca agggcttgca
     2281 ggaggtcacc taccagtaca cctacattga cggtcgcatg gtggacagga cccagatcgg
     2341 cgacccggtg gtgctgcagg agccggtgga ggagcatatc tcggtgggca cctatatctc
     2401 ccaggctggc tacagcgtca cccccagcgg cagcggcggc atgatcttcc cggtcgggcc
     2461 gggcttcacg tacatgagcc gcggcttcag cggggcttac gcccacaacg gcctggacct
     2521 ttgcgcggcc tacggcaccc ccatctatgc ggcgcagagc ggcgtggtca cctacgccag
     2581 cgccaccgcc ggcggctacg gcatccatgt catcatcaac cacggcggcg gggtgcagac
     2641 cctttacggg cactgcagcg ccctggtcgt gggcgcgggc cagtcggtgg cccgggggca
     2701 ggtcatcgcc tatgtgggca gcaccggcaa ttccaccggc aaccactgcc attttgaggt
     2761 tatcatcaac ggcgcgcggg tcaacggagc cccctatgtg ggcatctgag ccgaaaaaag
     2821 acaaaaacag ccggaagaat tttcttccgg ctgtttttct gtgccctgtt cggggaaccc
     2881 tgttaaggtg caaaaagaga gcctggcccg gcggaacggc aacaaatctc catgcggttt
     2941 ttctcatgaa attgttactt ttcatccccg tgcaaaggtg ctataataat acaggctctg
     3001 ttgagctctt aaaaagagag attacctgcc tgaaaaaggg gagtttggaa tattggagaa
     3061 attcgtggta cgcggcggca cgcctctggt gggcgaggtc accgtgagcg gcgccaaaaa
     3121 tgcagccgtc gccatcttgc ccgccaccat cctggcgggg ggcaaatgtg tgatcgagaa
     3181 cctgcccaac atcagcgatg tggccacctc ggtggagatt ttgcacgcca tgggggcgtc
     3241 gatccgcatg atcgacagct ccacgctgga ggtggatacc tcccgcatcg tggtcaccga
     3301 cgtgcccgcg gagatgagcc ggcagatgcg cgcctcctac tattttctcg gcgcgctgct
     3361 ggggcgcttc ggccgcggct gcgtctcgat gcccggcggc tgcgatctgg gcacccggcc
     3421 catcgaccag cacctgaaag cgttcggctc gctgggcgcc agctacaatg tggagtacgg
     3481 catgatccgg gtcagctcca gcgtgctgac ggctgagcac atctttttcg acaccgtctc
     3541 ggtgggggcc accatcaacg ccatgctggc ggccgtgatg gcagaggggc tcactgtcct
     3601 tgagaacgtc gccaaagagc cgcacattgt ggatgtggcg aactttttga acaccatggg
     3661 cgcggatgtg cgcggcgcgg gcaccgatgt gatcaagatc cgcggggtgc gcgagatgca
     3721 cggctgcacc tactcgatca tccccgacca gatcgaggcg ggcagctata tggccgcggc
     3781 caccatcacc cgcggcaacg tgctgatccg caacgtcatc cccaagcacc tggagcccat
     3841 caccgccaag ctggaaaagg cgggcgccac cattgaggag ttcgacgacg ccgtgcgggt
     3901 gtgctgtgtg ggcgacatcg agccgctgaa catcaaaacg atgccgcatc ccggctttcc
     3961 caccgacatg cagccgctga tggcggtgct gctgagcgtg gccaagggca cctccatcat
     4021 caccgagggc atctgggaga accgcttccg ctatgtggac gagctgatcc gcatgggggc
     4081 gaatatccag gtggacggcc gggtggccgt catcgagggg gtgcctgagc tgcagcccgc
     4141 gccggtgcgc gccaccgacc tgcgggcggg cgcggccctg gtggtggcgg cactggcggc
     4201 gggcggggtc tctgagattg aggagatcag ccatatcgag cgcggctatg aggacctggt
     4261 gggcaagctg cgcggcctgg gggcggatat caagcgcatc gacataccgg gggaacggtt
     4321 gcgccaggcg ctgtgaggcg gctttgcccc cgtgggacat atcaggacga aaaggggctg
     4381 tcccgtgcag gggcggcctt tattgataag gccccggcct tttccaaaaa acacttttta
     4441 ggagcttaca tatcatggcg actttcctct ccctctattc cggctcctcc ggcaactgcg
     4501 cgctggtggg cgaaaacggc aggtatctct gtgtggacat gggaaaaaac tgcaagacca
     4561 ccatcaacgc cctctactcg gcgggccttt cggtggcgga cctagacggc attctcatca
     4621 cccacgagca ctcggaccat gtcagcggcc tgcgggtgtt tctcaagcac tatcccgtgc
     4681 cggtctacgg cagcgaggcg acgctggcct atctggccga tcacggcatg gtgccgccca
     4741 cggcgcagct gcaggtgctt gacggcagcg ggcgggtggg ggagtttgag gtgcgctctt
     4801 ttcacaccag ccacgactcg gtggattgct gcggctttcg catcacggcg ccctcgggcc
     4861 gcacggcggc catggccacc gatctcggcg tcatcaccca agaggtgatg cagggcctgg
     4921 ccggggcgga tctggtggtg ttggaggcaa actacgacgc cgtgcggctg cgcacgggcc
     4981 actatcccta ttatctcaag gcgcgcatcg cctccccccg cgggcatctg tgcaacgcgg
     5041 agaccggcga gacgctgacc cggctgctgc agtgcggctg cgagcggttc g
//
LOCUS       DBMDYA010000122         5086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4042_length_5086_cov_1.483250, whole genome shotgun sequence.
ACCESSION   DBMDYA010000122 DBMDYA010000000
VERSION     DBMDYA010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_5086_cov_1.483250"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="ACZPFK_04235"
     CDS             complement(<1..188)
                     /locus_tag="ACZPFK_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405552.1"
                     /translation="MGDNEALQRWDAAAKTYREVQEQSAFAAQNRALVEQRFKEKVTG
                     WQVLDAGCGYGRYTRWFQ"
     gene            complement(312..830)
                     /locus_tag="ACZPFK_04240"
     CDS             complement(312..830)
                     /locus_tag="ACZPFK_04240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1405553.1"
                     /translation="MEQEILKVMKERRSVRAYKPEQITPKELDTVLEAGTWAPSSMGL
                     QSPKMVVVQDKETVQLLSKLNARVLGNDSDPFYGAPTVIVVFSNGDRPTCTEDGSLVM
                     ANLMLAAKAVGLGSCWIHRAKSVFESEEGKALKAKWGIPENYVGVAHCILGYAAEEPQ
                     AKPRKEDAIVRV"
     gene            complement(1008..2288)
                     /locus_tag="ACZPFK_04245"
     CDS             complement(1008..2288)
                     /locus_tag="ACZPFK_04245"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1405554.1"
                     /translation="MKTAFVNCTLLSGTKDMEPQAGMAVVVENGKITSVEKAAGAYEG
                     CEVVDLGGRYLMPGLINLHLHLPSTGAPKKKETDAAKLASFLMSNPITRALARSVCEK
                     AAKTELLSGVTTIRTVGGLDGVDSAIRDRIKAGKTVGPRMLVSDLAVTVPGGHMAGSV
                     AAVAQSEEECRALVRKIAQGKPDLIKLMITGGVLDAKVKGEPGVLRMPPELVRAACEE
                     AHKLGYQVAAHVESSEGVRVALENGVDTIEHGAPLDEALVELFKQRGAAHVCTISPAV
                     PLAMMDPEVVGSTELTQYNGNVVFEGIVSCAKTALANDIPVGLGTDAGCPFTTHYDMW
                     RELQYFHKYVGVSRAFALYTATLKNAEILGIQNETGSVEAGKSADMLITGGNPLEDLT
                     CLRAPYMVVMAGRVIAQPQVKKYPLVERELDKFL"
     gene            complement(2495..2710)
                     /locus_tag="ACZPFK_04250"
     CDS             complement(2495..2710)
                     /locus_tag="ACZPFK_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405555.1"
                     /translation="MCAKCGEVIALRHPKVFNAQTYEYCPLLPQALEHRDEIMARCGL
                     VPGGYGKAFSNGAASPHRERSGWKQPG"
     gene            complement(3006..3995)
                     /locus_tag="ACZPFK_04255"
     CDS             complement(3006..3995)
                     /locus_tag="ACZPFK_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405556.1"
                     /translation="MLDQQTIDEFVAFLSLGEKMEYNTRNILLRLSKYEADVPIEYFT
                     ADQFIGMFEKEGWVRFNHFKNIKSVIKKYILWRMYTDPDPLLSVKAIQAVHFAALDPT
                     ENFKRDYFGSFEDFYGFLTSLCGPCGDEEDPNLMFKVACSLLWLGLDLEEVERLPLGS
                     VDRVNGILTIGDYVVAVPPPLLELFQRASGATAVRVISASGKERRYPYCETGAIYKVV
                     ARKDNIVRTGGDSKNRQERLRRKYRQLKTMASPEECGMKKELKIQGIQFSGMACRYRS
                     YKTANKITGNTPKKIPQALLDDVARIFRIHLTKQSLETVLKKYEGWESTFYPQ"
     gene            complement(4335..4679)
                     /locus_tag="ACZPFK_04260"
     CDS             complement(4335..4679)
                     /locus_tag="ACZPFK_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405557.1"
                     /translation="MTEKLSEQALIDELAAFDRYECISVELDPFGRRAVSAHTVFDSF
                     EVERVGALLTKIFYNSDTGSSLTIFSEIACAEKAVVRGRGDDSFQYRLTLKDSGKTLF
                     ILAGKRRESEVF"
     gene            4934..>5086
                     /locus_tag="ACZPFK_04265"
     CDS             4934..>5086
                     /locus_tag="ACZPFK_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405558.1"
                     /translation="MDGFSCKLKEWRLASGLSQQQIADLLNYGLSTVQSWEAGRKLPS
                     YDALISL"
BASE COUNT          976 a         1601 c         1296 g         1213 t
ORIGIN      
        1 tcttgaaacc aacgggtata ccggccatag ccgcagccgg cgtccaagac ctgccagccc
       61 gtgacttttt ctttgaaccg ctgctctacc agggccctgt tctgggccgc aaatgccgat
      121 tgctcctgca cttcccggta ggtctttgcc gctgcgtccc accgctgcag agcctcatta
      181 tcccccatct gtgtgcactc ctctcttgtc ggccgaaaga agagctttac ttccccggct
      241 tttttaaggg caaacagcgc cgcccgcagg ggaggtctct caactgcagg cggcgctgtt
      301 ctctttcaag tttacacgcg cacgatcgca tcctctttgc gcggctttgc ctgcggctct
      361 tctgcagcat agcccaggat gcagtgggcc acgcccacat aattttcagg gatgccccat
      421 ttcgctttca gggccttgcc ctcctcgctc tcaaacaccg acttggcgcg gtggatccag
      481 caggagccca gccccacggc cttggcggcc agcatcagat ttgccatcac cagcgagccg
      541 tcctcggtgc aggtgggacg gtcgccgttt gagaacacaa cgatcaccgt gggcgcgccg
      601 taaaaggggt cgctgtcgtt gcccaggacc cgcgcgttca gctttgagag cagctgcacg
      661 gtctctttat cctgcaccac caccattttc ggcgactgca gccccatgct gctgggcgcc
      721 caggtgcccg cctccagcac cgtgtccagt tctttcggcg tgatttgctc cggcttgtag
      781 gcgcgcacgc tgcggcgctc tttcatgacc tttaaaattt cctgttccat tttcgtctcc
      841 cccattttct ggttttgtac acagccaaat tataacatta ccgccgggag aaatcaattt
      901 ttgcgcctcg cgtgggcggt gtcttgacgc tcttgcgctg aaaacagcaa ggcgggccgt
      961 ctgcactgca cgcacagcgg tccgccctgt ctgtaaaaag taaaagctta taaaaatttg
     1021 tccagctccc gctccaccaa cgggtatttt ttcacctgcg gctgcgcgat cacccggccg
     1081 gccatgacga ccatatacgg tgcgcgcagg caggtcaagt cctccagcgg gttgccgccg
     1141 gtgatcagca tgtcggcgga tttgcccgcc tccaccgagc ccgtctcatt ctggataccc
     1201 aggatctccg cgtttttcag cgttgccgtg taaagggcaa aggcacggga cacccccaca
     1261 tacttgtgaa aatactgcag ctcgcgccac atgtcgtagt gggtggtgaa cgggcagccc
     1321 gcgtcggtgc cgagccccac cgggatgtcg ttggccaggg cggtctttgc gcaggagaca
     1381 atgccctcaa acaccacgtt gccgttatat tgggtcagct cggtgctgcc caccacctcc
     1441 gggtccatca tcgccagggg cacggcgggg gaaatggtgc acacatgggc cgcgccgcgc
     1501 tgtttgaaca gctctaccag cgcctcgtcg agcggcgcgc cgtgctcgat ggtatccacg
     1561 ccgttttcca gcgccacccg caccccctcg ctgctctcca catgggcggc cacctgatac
     1621 cccagcttgt gagcctcctc acaggccgcc ctgaccagct cgggcggcat gcgcagcacg
     1681 cccggctcgc ccttgacctt ggcgtccagc acgcccccgg tgatcatcag cttaatcagg
     1741 tccggcttgc cctgggcaat tttgcgcacc agggcgcggc attcctcctc actctgcgcc
     1801 accgcggcca ccgagcccgc catatgcccg cccggaaccg tcaccgccag gtcggacacc
     1861 agcatgcgcg gccccaccgt cttgcccgct ttgatccggt cgcggatggc ggaatccaca
     1921 ccgtccaggc cgcccaccgt gcggatggta gtgacgccgg agagcagctc ggtctttgcc
     1981 gccttttcgc agacgctgcg cgccagcgcc cgcgtgatcg ggttgctcat caaaaaagag
     2041 gccagcttgg cggcgtcggt ctcctttttc ttcggcgcgc cggtggaagg cagatgcagg
     2101 tgcaggttga tgagacccgg catcagatac ctgccgccca ggtccaccac ctcgcagccc
     2161 tcataggcgc ccgccgcctt ttccaccgag gtgatctttc cattctcgac gacgacggcc
     2221 atcccggcct gcggctccat gtcctttgtg ccgctcaaca gggtgcagtt gacaaatgca
     2281 gttttcatcc tcatcacgct tcttccccaa agattgaaca cagtatagca gaaccggacc
     2341 cgtttttcaa cgaaacggcg gcagtctctt tcaaggtggt tcccttttat ccccggcctt
     2401 tcgggctttt ccccttttgg aggaagaaga actcgtttct tatactgaac cacgctattc
     2461 gccgcgccgg aggatggggc ttgggccttt tctttcagcc cggctgcttc catccgcttc
     2521 tttcccgatg cggggaggcg gctccattcg aaaacgcctt tccgtatccc cccggcacaa
     2581 gcccgcagcg cgccatgatc tcatcgcggt gctccagcgc ctgcggcagc agagggcaat
     2641 attcataggt ctgagcattg aacacctttg ggtgccgcag ggcgatcacc tcgccgcatt
     2701 tggcacacac cagggcgtca ccacgccatt cgcaatgccg ccaacagctc cccgctgcgg
     2761 cgtgaaatat tttagaaaaa cagttcaaat ccatcttcct ccctctcctg ttccgcgcgt
     2821 ttctggcaga gtgcccgaca tttttctctc ctgcttttcc cctctccccc tttcacgtcc
     2881 atcctgacat tctcgtttcc ccctgcgcca tcaagcgggc ggacctgcag gtgaaaaaac
     2941 gcgataattt attacttttt acaatttata taaattggaa acgcaggctc accgcccgcc
     3001 tttcttcact gcggataaaa agtactttcc catccctcat actttttcag caccgtctcc
     3061 aggctctgct tggtgagatg aatgcgaaaa atgcgggcga cgtcgtcgag aagcgcctgg
     3121 gggatctttt tgggcgtgtt gccggtgatc ttatttgcgg ttttataact gcgataacgg
     3181 caggccatgc cgctgaactg gatcccctgg atcttcagct cttttttcat cccgcattct
     3241 tctggactcg ccattgtttt cagctggcgg tattttctgc gcagcctttc ctgccggttt
     3301 ttggaatcgc caccggtacg gacgatgttg tctttccgcg ccacgacctt gtagatggcc
     3361 cccgtctcac aataggggta ccgcctctct tttccggagg cggaaatcac cctcaccgcc
     3421 gtcgccccgc tcgccctttg aaacagctcc aacaggggcg gcggcacggc gaccacatag
     3481 tccccgatgg tcaagatccc gttcacccgg tctacggagc caagagggag acgctccacc
     3541 tcctccaggt ccagcccgag ccaaagcagg gagcaggcca ccttgaacat caggttgggg
     3601 tcctcctcat cgccgcaagg cccgcagaga gaggtgagaa agccgtaaaa atcttcaaaa
     3661 gagccaaaat aatcgcgttt gaaattttca gtggggtcaa gcgcggcaaa gtggacggcc
     3721 tggatcgcct tgacggaaag gagagggtcc gggtcggtgt acatcctcca aagaatgtac
     3781 ttttttatca cggatttgat atttttgaaa tgattgaagc ggacccagcc ctctttttca
     3841 aacatcccga tgaactgatc ggcggtaaaa tattcgatgg ggacgtcggc ctcatatttg
     3901 ctcagccgaa gcaggatatt gcgcgtattg tattccattt tttcgccgag ggacaaaaac
     3961 gcaacaaatt cgtcgatcgt ctgctgatcc agcacgggat cacctcctgt gtgttgtgag
     4021 cgttttgcgc tccgccccgc ctaaaaggcc gccagccccc tgaaaccgtt tttgcgcggg
     4081 cacttccgcg tgcccgccat ccaaaagcaa ttttactttt agaatgtcat attattgcgg
     4141 ccggcctggt ttcgctttga ttatattata tcagcagatg attttgtgtc aataatttta
     4201 ttacttttta taatttattt ggcgatgccc ggggattcac cgccgccggg gacgaaacct
     4261 cgcctctcac aaaaagcgca aaagccgccg ggcaccgcgg caaagggacg ggcgttatcc
     4321 cctatcctct tttttcaaaa cacctcgctt tctctccttt tccccgcgag gataaaaagc
     4381 gttttgccgc tgtcttttaa tgtaaggcga tactgaaagg aatcatcgcc ccggccccgg
     4441 accaccgctt tttcagcgca ggcgatctcg gaaaaaatcg tcaggctgga acccgtgtca
     4501 gaattataaa atatctttgt cagcagcgcc cctacccgct ccacttcaaa cgaatcaaaa
     4561 acggtgtggg cggagacggc gcgccgcccg aacgggtcca gctccaccga gatgcattcg
     4621 tatctgtcaa aggccgccag ttcgtcgatc aaggcctgct cgctcaattt ttctgtcacg
     4681 ggggaagtct cccccaaccg ctgggtcttt tttgcgctca tcagctgcct ccgtcttttc
     4741 ttgacaaaca ttccaagctt ttttgtgcaa agagcatgtt ttcttatcaa gcgggttgtg
     4801 gtatactttg gttgttataa cttcttatat tagatttctt tacatcaatt agttgttttt
     4861 acatatttat cttagcacgt ttttccatct tatgccactt accttagtga ggatgagaga
     4921 gggaggctgt aaaatggatg gattctcctg caaactgaaa gagtggcgtc ttgccagcgg
     4981 cctgtctcag cagcagatcg ccgacctgct caactacggc ttgagcaccg tccagtcgtg
     5041 ggaagcaggc cgaaagctgc ccagctatga cgcgctcatc tcgctg
//
LOCUS       DBMDYA010000123         5082 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4046_length_5082_cov_1.329653, whole genome shotgun sequence.
ACCESSION   DBMDYA010000123 DBMDYA010000000
VERSION     DBMDYA010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4046_length_5082_cov_1.329653"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(203..278)
                     /locus_tag="ACZPFK_04270"
     tRNA            complement(203..278)
                     /locus_tag="ACZPFK_04270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Thr,seq:cgt)
     gene            410..769
                     /locus_tag="ACZPFK_04275"
     CDS             410..769
                     /locus_tag="ACZPFK_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405559.1"
                     /translation="MIVESVAIFGITVCILIVFVRSGNADYAIATLPLLIVPAANVLG
                     WPVASLVNNMMPGNQLYLVRCFVDVLGVAIACGLIVLFSGKVKAKKKKQLYIGMCSLY
                     CIVLTCVFIYNALPKLG"
     gene            complement(872..2242)
                     /locus_tag="ACZPFK_04280"
     CDS             complement(872..2242)
                     /locus_tag="ACZPFK_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1405560.1"
                     /translation="MKQHYDVAIVGGGIGGLMSAHRLITSDPGLKIALFEKGHDLDHR
                     SCPIVTGKAATCVRCPSCAIMEGVAGAGAFSDGKYVISTEYGGWLPDLLPAKTVLEYI
                     EAADGVLMSFGAPKDRFQPDDELKKLCLEHDLHMSQAQLKHLGTDANFETMRRMSEFL
                     AQRIDLFTDTNVTDVDKGSHTLTFEGRENGAVTADRIILAVGRVGSRGFADWCHKNGI
                     PLTNNQVDIGVRVELPAIVWEDFSKKIYEPKIWYRSKQYGDVTRMFCFNERGNVVMEN
                     TAGVLTVNGHSYKDECRKTQNSNFALLSTIRFTEPFKEPIEYARYVASLANLISGGGV
                     LVQRLGDLLAGRRTDDKRLAQSTTRPTLNAVAGDLSLCLPKRQLDNILETLEALDKVA
                     PGTMNYDTLLYGIECKYYSARPACDDFELQGCKGIYAVGDGTGFTRSLSQAAAHGLYV
                     ADKILG"
     gene            complement(2257..3441)
                     /locus_tag="ACZPFK_04285"
     CDS             complement(2257..3441)
                     /locus_tag="ACZPFK_04285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1405561.1"
                     /translation="MKIAIFAETYYPFISGVVTHIETLKNGLEAQGHQVLIVTMNPKA
                     SHHYLKDGVLYCPSIPLKKIYGYGVANPVNLRRLSYIRAFNPDIIHIQTEFTMGLFGL
                     FCARQLKKPVVYTLHTMYDDYLFYVAPSERMQKVVKPGAHWYFKNVAKKATEIIGPSP
                     KVVEFLRRCGVERHINIIPNTVDLSDFMAENIDPAKVQQTREKLGIRPGDVSMLFVGR
                     LGKEKSLDVLVDLFAENFAGEERFKLFLIGDGPEAEALRGQIAARGVEKQVRLLGRID
                     HDLLPPYFQACDLFTTASLSEINSISMLEAMASGLYVVQRLDLYNREQIDQGKNGDIF
                     VDAPGYAKIIRGWDALSADEKSALRAGVSDTTRRYGEKEFTRSVINVYQRAVYEYSMK
                     IK"
     gene            3689..4618
                     /gene="hprK"
                     /locus_tag="ACZPFK_04290"
     CDS             3689..4618
                     /gene="hprK"
                     /locus_tag="ACZPFK_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1405562.1"
                     /translation="MEFSVSLAKLVNDLGLEVVYTPCDVDKIRITSADVNRPGLLLAG
                     YHEYFDPTRIQICGKVEMSFLEALPENERRSRIHTLFATKPVAVAVARSLYIYDSMLE
                     FATQYGVPVLSAAESTSRLMSDIISILGVELAPRVTRHGVLVEVYGEGILILGESGVG
                     KSETAVELVKRGHRLIADDAVELRRVSNRTIVGTAPENIRHFIELRGIGIINVARVFG
                     SGAVKASERVDLVVELQPWDKTKNYSRTGLESETIELLGLEVPYTVVPVHPGRNLAVI
                     LETAAICNRQKKMGYNAAEELMAKLGLENDISE"
     gene            4726..>5082
                     /locus_tag="ACZPFK_04295"
     CDS             4726..>5082
                     /locus_tag="ACZPFK_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1405563.1"
                     /translation="MIDYQQLAGQMEAAGVHARLQEPLSRHTTFRIGGPARLFCLPAS
                     ENGLIAALELCRAAGCRTYLLGKGSNTLFSDEGFEGAVIALGMGLDGVRAEGGGGLTA
                     GCGASLAQVCLFAARKG"
BASE COUNT          931 a         1504 c         1626 g         1021 t
ORIGIN      
        1 tgccggaccc cctccgccac tgcatgacag ctcccctgaa tttggttata tcgcggcaaa
       61 aaggcgcgcg ccccgcaaaa gcgccgcttt cagccggggg cggaaccgcg tttttttcta
      121 tcgaccccgg tcggcaaaag cagggcagcc cacaaaaaag aagaccgcac ggacgctttt
      181 tcaagcggct tgtgcggtct tctggagctg gaaacgtgac tcgaacacgc gacctgctga
      241 ttacgaatca gctgctctac caactgagct attccagcgc cctttttgcc ggccccatcg
      301 gcacggcaat tattattata ccaactttct ctgtttagtc aagaaatgct ttacgaaaac
      361 tttgttttgg ggtaaaatag acccgtgaaa agaggagggg ggagacggga tgatagtgga
      421 gagtgtggcg atttttggga tcacggtctg catcctgatc gtttttgtgc gcagcggcaa
      481 tgcggattat gccatagcca ccctgccgct gctcatcgtg ccggcggcga atgtgctggg
      541 ctggccggtg gcgagcctgg tcaacaatat gatgccgggc aaccagctct atctggtgcg
      601 ctgctttgtg gatgtgctgg gggtggccat cgcctgcggg ctgatcgtgc tcttctcggg
      661 caaggtaaaa gcgaaaaaga aaaagcagct ctatatcggc atgtgctctt tgtactgcat
      721 cgtgctgacc tgtgttttca tctacaacgc cctgcccaag ctggggtgac aaaaagacga
      781 aagaacgccc ggccctgcga ggatgcagcg gccgggcgtc tttttatctg acgaatgcct
      841 aatgcggcgg acgaaaggag agagacggga ttcagcccag aattttgtcg gccacataaa
      901 gcccgtgggc cgcggcctgg gagagcgagc gggtgaagcc ggtgccgtcg cccacggcgt
      961 agatgccctt gcagccctgc agttcaaagt cgtcgcaggc gggccgggcg gaatagtatt
     1021 tgcactcgat gccgtagagc agggtgtcgt agttcatggt gcccggcgcc accttgtcca
     1081 gcgcctccag cgtctcgagg atgttgtcca gctggcgctt gggcaggcag aggctcaaat
     1141 cgcccgccac tgcgttcagg gtggggcggg tggtgctctg tgccagccgc ttgtcgtcgg
     1201 tgcggcgtcc ggccagcagg tcgcccaggc gctgcaccaa cacgccgccg ccgctgatga
     1261 gattggcgag gctggccaca tagcgggcgt actcgatggg ctctttgaaa ggctcggtga
     1321 agcggatggt ggacagcagc gcaaagttgc tgttctgggt cttgcggcac tcgtccttat
     1381 acgagtggcc gttgacggtc agcacaccgg cggtgttctc catcaccacg ttgccccgct
     1441 cgttgaagca gaacatccgc gtcacatcgc cgtactgttt ggagcggtac cagatcttgg
     1501 gctcgtagat ctttttggaa aaatcctccc acactatggc gggcagctcc acccgaacgc
     1561 cgatgtccac ctggttgttg gtgaggggga tcccgttttt gtggcaccaa tccgcgaacc
     1621 cacgcgagcc cacccggccc acggccagga tgatgcggtc cgccgtgacg gcgccgttct
     1681 ctctgccctc aaaggtgagg gtgtggctgc ccttgtccac atcggtgacg ttggtgtcgg
     1741 tgaacaggtc gatgcgctgg gcgagaaact cgctcattct gcgcatggtc tcaaagttcg
     1801 cgtcggtgcc caggtgcttg agctgcgcct gggacatatg caaatcgtgc tccaggcaga
     1861 gctttttcag ctcgtcgtcc ggctgaaagc ggtctttcgg cgcgccaaag gacatcagca
     1921 ccccgtcggc cgcctcgata tactccagca ccgtctttgc gggcagcagg tcgggcagcc
     1981 agccgccgta ttcggtggag atgacatatt tgccgtctga aaaggccccc gcgcccgcga
     2041 ccccctccat gatggcgcag gaggggcagc gcacgcaggt ggcggctttg ccggtcacga
     2101 tggggcagct gcggtgatcc aggtcatggc ctttttcaaa cagggcgatc ttcaggcccg
     2161 ggtcgctggt gatgaggcgg tgggccgaca tcagcccgcc gatcccgccg cccacgatgg
     2221 cgacgtcata atgctgtttc atggatgatg ctcctgtcat ttgattttca tgctgtattc
     2281 gtagaccgcg cgctgataga cgttgatcac cgagcgggtg aactcctttt cgccgtagcg
     2341 gcgggtggtg tccgacacac cggcgcgcag ggcgctcttt tcgtcggcgc tgagggcgtc
     2401 ccagccgcgg atgatcttgg cgtagcccgg ggcgtcgaca aagatgtcgc cgtttttgcc
     2461 ctggtcgatc tgctcgcggt tatacaggtc cagccgctgc accacataca ggccgctcgc
     2521 catggcctcc agcatcgaga tggagttgat ctccgagagg gaggcggtgg tgaagaggtc
     2581 gcaggcctgg aaatagggcg gcagcaggtc gtggtcgatg cggcccagca ggcgcacctg
     2641 cttttccacc ccgcgggcgg cgatctgccc gcgcagggcc tccgcctcgg gcccgtcgcc
     2701 gatgagaaac agcttgaacc gctcttcccc ggcaaagttt tcggcaaaaa ggtccaccag
     2761 cacgtccagg ctcttctcct tgcccagcct gcccacgaac agcatcgaga cgtccccggg
     2821 ccggatgccc agcttctccc gcgtctgctg cactttggcg gggtcgatgt tctcggccat
     2881 aaaatcgctc aggtccaccg tgttggggat gatgttgatg tggcgctcca cgccgcagcg
     2941 gcgcaagaac tccaccacct tgggcgaggg gccgatgatc tcggtggcct ttttcgccac
     3001 gtttttgaaa taccagtgcg cccccggttt gaccaccttt tgcatccgct cgctgggcgc
     3061 cacatagaac agatagtcgt cgtacatggt gtgcagggta tacaccaccg gttttttcag
     3121 ctgccgggcg cagaacaagc cgaacaggcc catggtgaac tcggtctgga tatggatgat
     3181 gtcggggttg aaagcgcgga tataggacag ccgccgcaga ttgacggggt tggccacgcc
     3241 gtagccgtag atctttttca gcgggatgga cgggcagtag agcacgccgt ctttcaggta
     3301 atggtggctg gctttggggt tcatggtgac gatgagcacc tggtggccct gggcctcaag
     3361 gccgtttttc agcgtttcga tatgggtgac cacgccgctg atgaacgggt aataggtctc
     3421 tgcaaaaata gcgatcttca taaggggaat cgcacctcac tttttcactt cggctgccgg
     3481 gcaaaaaccg gggggatttt gccgaaaagc agcccgttct tttcaataaa acgatgcgcc
     3541 gcaaaaaaat ccgacatgcc cattataaca gaccagcatt ttagtgcaac cgtttgttgc
     3601 actgaggggc ctttctatac tataatattg tcagtaccgt gtcgctatcc gcaccggcgg
     3661 gcggcgcgtg cagaaagagg ttgtgaaaat ggaattttca gtcagtctcg ccaagctggt
     3721 caacgatctc ggcctggagg tcgtctacac cccctgcgac gtcgacaaga tcaggattac
     3781 ctcggccgac gtcaaccggc ccggcctcct gctggcgggc tatcacgagt atttcgaccc
     3841 cacccgcatc cagatctgcg gcaaggtgga gatgtccttt ttggaggcgc tgcccgaaaa
     3901 tgagcggcgc agccgcatcc acaccctgtt cgccaccaag ccggtggcgg tggcggtcgc
     3961 ccgcagcctc tatatctacg attccatgct ggagttcgcc acccagtacg gcgtgccggt
     4021 cctctcggcc gcagagagca cctcgcgcct gatgtccgac atcatctcga tcctgggggt
     4081 ggagctggcc ccgcgcgtga cccgccacgg cgtgctggtg gaggtctacg gcgagggcat
     4141 cctcatcctg ggcgagagcg gcgtgggcaa aagcgagacc gcggtcgagc tggtcaagcg
     4201 cggccaccgg ctgatcgcgg acgacgcggt ggagctgcgc cgggtctcga accgcaccat
     4261 cgtcggcacc gcgcccgaaa acatccgcca tttcatcgag ctgcgcggca tcggcatcat
     4321 caacgtggcg cgggtgttcg gctccggcgc ggtcaaggcg tccgagcggg tggatttggt
     4381 ggtggagctg cagccctggg acaagaccaa gaattacagc cgtaccgggc tggaatccga
     4441 gaccatcgag ctgttggggc tggaggtgcc ctataccgtc gtgccggtgc accccggccg
     4501 caacctggcg gtgattttgg agaccgccgc catctgcaac cgtcagaaga agatgggcta
     4561 caacgccgcc gaagaattga tggcaaaact gggactggaa aacgacatca gcgagtaaac
     4621 tagaagagag ggccggctgg cccgccctgc gagcgcggcg catcccggcg gggatgcgct
     4681 gtgcggtttt ttgcaggggc atcactgcgt catgggagga aacggatgat cgactaccag
     4741 caactggcgg gccagatgga ggctgcgggg gtacacgcgc gcctgcagga accgctttcc
     4801 cggcacacca ctttccgcat cggcggcccg gcccggctgt tctgcctgcc cgcctctgaa
     4861 aacggcctca tcgcggcgct ggagctctgc cgcgccgccg gctgccgcac ctatctgctg
     4921 ggcaagggct ccaacacgct gttcagcgac gagggctttg agggcgcggt gatcgccctg
     4981 ggcatggggc tggacggcgt gcgggccgag ggcggcggcg ggctgacggc gggctgcggg
     5041 gcctcgctgg cccaggtctg cctttttgcg gcccgcaagg gg
//
LOCUS       DBMDYA010000124         5073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4052_length_5073_cov_1.695897, whole genome shotgun sequence.
ACCESSION   DBMDYA010000124 DBMDYA010000000
VERSION     DBMDYA010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4052_length_5073_cov_1.695897"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="ACZPFK_04300"
     CDS             <1..1486
                     /locus_tag="ACZPFK_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1405564.1"
                     /translation="QAAAHARQALERDVRHPLAPAALLLACAGLGRAEEARQVAAAAL
                     AHDPFNLLVRALSDLPREQFYGEMHSSPAQSVLDLVSDLDAMGQRDHILALLEGLLRH
                     RPEEGCAMILFTLAYYRGLTGGDCTELLRRAERAPLGQTYPFRSIEARALRAAMERGS
                     ARAPFLLGCLLYDKRHYQQAAELFEEALRRDPDNYMACRSLAVACFSHLGRRRDALPL
                     MHRAMSLHPGAQLLYETAVLMDKLGRPAAEKIALLEKDPQLLRRDDLLTELAKAYNQN
                     GQPGRALEILAAHTFVPCEGGEHAIADQYMFAHYLRGMERLAAGEPEKAEACFTAALT
                     LPENLGAGIWNRCKYVPYRYRLAECLELLGRGAEAAALYREILEIQVDFFSNMHLREL
                     PYYQAMAARKLGQEQRACAILAPARRAWAAELERVDNGFFSTTPFFISFTDDPAQLRR
                     AQYLYLLALAELFQGRDRRAGELFRESLALNSDNLFCGYFAQAL"
     gene            2169..3017
                     /locus_tag="ACZPFK_04305"
     CDS             2169..3017
                     /locus_tag="ACZPFK_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1405565.1"
                     /translation="MNKSLLTLRAAGWTAQIAPGLGGNLTRLEYLGRPVLRPLEDPAQ
                     LTADPFLFGSPILFPANRTRAGRFTFEGAVYQLPVNEPQHDAHLHGLVHLQAFAVLSC
                     AEDEAALCFENQARVYPFPFRLTVRYRLSGRGAESFYLIENTGSRAMPFTFGLHTTFP
                     EPEVFFVPLAAAQEKDAHHLPTGRILPLSPAERKCLSGAASRGQTHSGYYLSGGDTAR
                     IGPVHFQVSGAFDHWVLYNARGSAGLLCVEPQCGAVDGLNQQNGCRILQPGQQLRLST
                     RLTCPG"
     gene            3096..4196
                     /locus_tag="ACZPFK_04310"
     CDS             3096..4196
                     /locus_tag="ACZPFK_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HIR1405566.1"
                     /translation="MPPLTVLLKPASGSCNMRCSYCFYADETQNRATENYGMMSEATL
                     EAVVRECLARAEGSCSFGFQGGEPTLRGLGFFRRCTALVEQYRPSGLAVSYFLQTNGL
                     LLDESWAAFFREHRFLVGLSLDGDKELHDACRPDAAGKGTFNRVLRAARLLDRFGVEY
                     NVLTVVTARTARSIRRVYRFFMKNGLVYQQYIPCLDPLGEPRGLHPWSLTPEAYGEFL
                     IRLFDEWYADRRAGRFVYIRYFENLAALMLGRPPESCGMLGYCGPQLVVEADGSAYPC
                     DFYMLDDYRLGNFNTDGLPGMEARREEVGFLKESFLGLERCRACRWARLCRGGCRRDR
                     QGVGLHEIGQNYFCHAYLRFFEYAAPKLATLL"
     gene            complement(4535..>5073)
                     /locus_tag="ACZPFK_04315"
     CDS             complement(4535..>5073)
                     /locus_tag="ACZPFK_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HIR1405567.1"
                     /translation="EGLDGTVFFVTGSFADDRLESEMAKHGIAFYFLKPFDPAVLAAR
                     AASALERVAPPCAASDDECTVSEILREVGVPAHLKGYRFARQAILLIAEDESYLNGVT
                     KRLYPDVARSNNTTASRVERAIRHAITVAWERGGGEALHDYFGYTAYSQNGKPTNSEF
                     VAAIADKIRLDKKNRRQA"
BASE COUNT          821 a         1742 c         1584 g          926 t
ORIGIN      
        1 ccaggcggcg gcccacgcgc gccaggccct ggagcgggat gtccggcatc cgctggcccc
       61 ggcggcgctg ctgctcgcct gcgccgggct ggggcgcgcc gaggaggcgc ggcaggtcgc
      121 tgccgcggcg ctggcccacg acccgttcaa cctgttggtg cgcgcgctgt ccgacctgcc
      181 gcgagagcag ttttacggcg agatgcacag cagccccgcc caatcggtgc tggacctggt
      241 ctcagacctg gacgccatgg ggcagcgcga tcacatcctc gccttgctgg aggggctgct
      301 gcgccaccgc cccgaagagg gctgcgccat gatcctgttc acgctggcct attaccgcgg
      361 cctgacgggc ggcgactgca cagaactgct gcggcgcgcc gagcgcgcac cgctggggca
      421 gacctatcct ttccgctcca tcgaagcgcg tgccctgcgc gcagccatgg agcggggctc
      481 ggcgcgggcg ccttttctgc tgggctgcct gctttacgac aagcgccatt accagcaggc
      541 ggcggagctg tttgaggagg ccctgcgccg ggaccccgac aactacatgg cctgccgcag
      601 cctggccgtg gcctgcttca gccacctcgg ccgccgccgg gacgccctgc ccctcatgca
      661 ccgggccatg tccctgcacc ccggcgcgca gctgctctac gagacggcgg tgctgatgga
      721 taagctgggc cgccccgcgg cggaaaagat cgccctgctg gaaaaggacc cccagctgct
      781 gcgccgcgac gacctgctca ccgagctggc caaggcgtac aaccagaacg gccagccggg
      841 acgggcgctg gagattctcg ccgcccacac ctttgtgccg tgcgagggcg gcgagcacgc
      901 catcgccgat cagtacatgt tcgcccacta tctgcgcggt atggagcgcc tggcggcggg
      961 tgagccggag aaagccgagg cctgctttac cgcggccctc acgctgccgg aaaacctggg
     1021 ggcgggcatc tggaaccgct gcaaatacgt gccctaccgc taccgcctcg ccgagtgcct
     1081 ggagcttctg ggccgcggcg cagaggcggc cgccctctac cgggagatct tggagatcca
     1141 ggtggacttt ttcagcaaca tgcacctgcg ggagctgccc tactatcagg ccatggccgc
     1201 ccgcaagctg ggccaggagc agcgcgcctg cgccatcctt gccccggccc ggcgtgcatg
     1261 ggccgcggag ctggagcggg tggacaacgg ctttttctcc accacgccct ttttcatctc
     1321 tttcaccgac gacccggccc agctgcgccg cgcccaatat ctctatctgc tggcgctagc
     1381 ggagctgttc cagggacggg atcgccgcgc gggggaactg ttccgcgaga gcctcgcgct
     1441 caacagcgac aatctcttct gcggatattt cgcccaggcg ctgtgagcgc cgcgccctgt
     1501 ttttgcgatc tcttctcttt taccgacttg ctgcgcaaga cggtaaaaac ggtcttgtag
     1561 gccctcccgg ccacggtctt aaaagccaaa cgcctgcctg taccgcggcg aaagcgctcg
     1621 aaaaacagcg gtctgtcctg atgaccccgg cccgggaggg ctgaacagcc gaaaaacggc
     1681 cccatgggcc gtcccggccc acggccttaa aatctgagca cccgcccatg ggagtggacg
     1741 gatgcgcgta aaaaccggcc tgcggctatt ttttattgct gcgcaataaa aaacgatcta
     1801 tcttaaatgg ccctgtttct cgccgctgcg cggcaaccga aacggatggc caaaaaaata
     1861 gcctgcggct attttttatt gctgcgcaat aaaaaacgat ctatcttaaa tggccccgtt
     1921 tctcaccgct gcgcggcaac cgaaacggat gaccgaaaaa atagcctgcg gctatttttt
     1981 actgctgcgc aataaaaaac gacttatttc tatggccgtg gcccaggaat gggccacatg
     2041 gccgaaaaaa tagcctgcgg ctatttttta ctcttatccg cttcagcggg ggatcccagt
     2101 cccccgctga agcgcgtcca ggaggcgggc gcccggcttc cccctcgcaa aagaaaggag
     2161 cttccccctt gaacaaatct cttttgaccc tgcgcgccgc gggctggacg gcccagattg
     2221 cccccggcct gggcggcaac ctgacccggc tggagtatct gggccgcccg gtgctgcgcc
     2281 cgctggagga cccggcccaa ctgaccgccg accccttttt gttcggcagc ccgatccttt
     2341 tccccgcaaa ccgcacccgc gccgggcggt tcacctttga gggggccgtc tatcaactgc
     2401 cggtgaacga gccccagcat gacgcccatc tgcacggcct ggtgcatctt caggcctttg
     2461 ccgtcctcag ctgtgccgag gacgaggcgg cgctctgttt tgagaaccag gcccgggtct
     2521 atccgttccc tttccggctc accgtgcgct accggctctc cggccgcggc gcggagtcct
     2581 tttacctcat cgaaaacacc ggcagccgcg ccatgccttt cacgtttggc ctccacacca
     2641 cctttcccga gccggaggtc ttttttgtgc cgctggccgc cgcgcaggaa aaggacgccc
     2701 accacctgcc caccggccgc attctgcctc tctcgccggc cgagcggaaa tgcctttccg
     2761 gcgcggcgtc ccgggggcag acgcactccg gctactacct gtccggcggg gacaccgccc
     2821 gcatcggccc ggtccatttt caggtctcgg gcgcgttcga ccactgggtg ctctacaacg
     2881 cccgcggcag cgcggggctg ctctgtgtgg agccccagtg cggcgcggtg gacggcctca
     2941 accagcaaaa cggctgtcgt atcctacagc ccgggcagca gctgcgcctt tccacccggc
     3001 tcacctgccc gggctgagtc ccggcggcgc tgtacttttg cgctcgtttt cgctataatc
     3061 agacgcaaga agaccacaaa aaggaggcgc gttgcatgcc gccgctcacc gtactgctca
     3121 agcctgcctc cggcagctgc aatatgcgct gcagctactg cttttacgcg gacgagaccc
     3181 aaaaccgcgc cacggaaaat tacggcatga tgagcgaggc cacgctggaa gcggtggtgc
     3241 gcgagtgcct tgcccgcgcc gagggcagct gcagcttcgg ctttcagggc ggcgagccca
     3301 ccctgcgcgg gctgggcttt ttccgccgct gcaccgccct ggtggagcaa taccggccca
     3361 gcgggttggc ggtctcttat tttttgcaga ccaacggcct gctgctggac gagagctggg
     3421 ccgcgttctt ccgcgaacac cgctttctgg tggggctgtc gctggacggc gacaaggagc
     3481 tgcacgacgc ctgccgcccc gacgcggcgg gcaagggcac cttcaaccgg gtgctgcggg
     3541 ccgcccgtct tttggaccgc tttggcgtgg agtacaacgt gctgaccgtg gtgacggcgc
     3601 gcaccgcgcg cagcatccgc cgcgtctacc gcttttttat gaaaaacggc ctggtctacc
     3661 agcaatatat cccctgcctc gacccgctgg gggagccgcg gggcctgcac ccctggtcgc
     3721 tgacgcccga ggcctacggc gagtttctca tccgcctttt tgacgagtgg tacgccgacc
     3781 gccgggcggg ccgctttgtc tatatccgct attttgagaa cctggcggcc ctgatgctgg
     3841 gccgcccccc tgagagctgc ggcatgctgg ggtactgcgg cccgcagctg gtggtggagg
     3901 cggacggcag cgcctatccc tgcgattttt atatgctgga cgattaccgc ctgggcaact
     3961 tcaacaccga cggcctgccc gggatggagg cccgccggga ggaggttggc tttttaaagg
     4021 aatcttttct ggggctggag cgctgccgcg cctgccgctg ggcccggctc tgccgcggcg
     4081 gctgccggcg cgaccgccag ggcgtgggcc tgcatgagat cggtcaaaat tacttctgcc
     4141 acgcctatct gcgctttttt gaatacgcgg ccccaaagct tgccacactg ctctgacgcg
     4201 gcgcgctgcg cccacaaccg cgcccggcgg ccctgcgcgg ccccgcgctc cctacctccc
     4261 gcttccccgg cgcaggacaa aggcgtttct cccccacgca gcggggctct ggacagatcg
     4321 ggccgcgcgg aaagcgctct cttttttgca gaggagcgca aaatgcgggc ggcgctttga
     4381 gggcctgatc cggcgccgcc gaaaaaggct ccacgatttt tagggcatga ccctgccgac
     4441 agattttact gcacaaaaga acgaccgtcc gatgcggaaa gcattggccg gtcgttttct
     4501 tttctatcgg cggcgacggc gtgggggagg tcttttacgc ctgccggcgg ttctttttgt
     4561 ccaaccggat cttatccgcg attgccgcca caaattccga attggtgggc ttgccgttct
     4621 ggctgtaggc ggtgtaaccg aaataatcgt gcagcgcctc gccgccgccc cgctcccagg
     4681 cgacggtgat ggcgtggcgg atggcccgct ccacccggct ggcggtggtg ttgttgctgc
     4741 gcgccacatc cgggtagagc cgtttcgtca ccccgttgag gtagctctca tcctcagcga
     4801 tgagcaggat ggcctgccgg gcgaagcggt agcccttgag atgcgcgggc acccccacct
     4861 cgcgcaggat ttccgatacc gtgcactcgt cgtccgaggc agcgcagggg ggcgcaaccc
     4921 gctccagcgc cgaggccgcg cgggccgcca ggacggcggg gtcaaacggc ttgaggaaat
     4981 aaaaggcgat gccgtgtttt gccatctcac tctccagccg gtcgtccgca aaactgccgg
     5041 tgacaaaaaa caccgtgccg tcaagcccct cgg
//
LOCUS       DBMDYA010000125         5057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4069_length_5057_cov_1.437651, whole genome shotgun sequence.
ACCESSION   DBMDYA010000125 DBMDYA010000000
VERSION     DBMDYA010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_5057_cov_1.437651"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="ACZPFK_04320"
     CDS             <1..176
                     /locus_tag="ACZPFK_04320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1405568.1"
                     /translation="RGLARACAARLILECLDRGLYPSWDAHDARSLALAESLGYRSAG
                     PYPAYERVGPAGE"
     gene            complement(218..484)
                     /locus_tag="ACZPFK_04325"
     CDS             complement(218..484)
                     /locus_tag="ACZPFK_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012472.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1405569.1"
                     /translation="MWGTVSAPRARLAQKYGCTPAQIALRWDLQRGFAVIPKSTQCGR
                     IFENAAILDHPLEEVDLALPDGLPTGWRTSAKGPAAFDFDLFLL"
     gene            complement(527..874)
                     /locus_tag="ACZPFK_04330"
     CDS             complement(527..874)
                     /locus_tag="ACZPFK_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012472.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1405570.1"
                     /translation="MLQVCGAPREDLSPDGKPDLYSTRYDRVLSSFEESLSRLRSDRL
                     DLFFIHRPDQQRGRLLDARRAMERLYEERRALGVCNCTVRRLEWLLEEDWVPPAVNQV
                     ERHPLLNETALYA"
     gene            complement(1094..2116)
                     /locus_tag="ACZPFK_04335"
     CDS             complement(1094..2116)
                     /locus_tag="ACZPFK_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405571.1"
                     /translation="MANATIKDVAREAGVSIATVSRVLNKSSTVREDLADRVNSAIEK
                     LGYYPNSIARTLKNDSSKTVGFVVSDIANDFFTSMVRSVEDVLNQRGYNLFVCSTDSS
                     QQRELDYLSLLREKQVDGIIINISGKNNELITRMSQHLPIALFGRKVPDAGFKGDFVD
                     NDNFTGMVDLTKQLISLGHRRIGLINGQPYVSSAKERLEGFQSAMRTIGVTVDDAYPY
                     LYHGNFNRISSGVEGARALYERGATAVLAANNLLALGALQFCREAQVEVPGKLSLSSF
                     GRISNHDFLYVQPTCVDQFPAAMGSRLAELIIERIESKNTLLNREIRFATTLIPGNGT
                     ARPCEG"
     gene            2525..3673
                     /locus_tag="ACZPFK_04340"
     CDS             2525..3673
                     /locus_tag="ACZPFK_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1405572.1"
                     /translation="MKKRILASLAAATLICTLLTGCGGSGASGDVIKIGLTVPLSGDR
                     ASEGSYASNAAEIVEKEINDAGGVLGKNIKIEIQDSLGTDVGATNAYLKLAADKDVVA
                     IIGPDNSNDNIAIASSAESSQILTTGQGSSPTLQTTCEEGEWMFQLRACDSTLCAALM
                     DYAYTEVGAKSFTIIHDTETASADQARLFQEAIEALGGTVDGVVSYTSGTKDFTAQLT
                     QAAANDSDALVMACLYTEAAIIIQQTRDMGIDKPVFGSNAFGDPIAIGLAGEAINDVY
                     SVTAWVPNTPNPLGAAFSKKYSEQFNEDCAKAAAQIRDHVYVICAAIEKAGSTDRAAV
                     RDAMLQLTDYEGAITTYNCSRRGNCGLGGLIVQVQDQTPTIIKEITTE"
     gene            3829..4695
                     /locus_tag="ACZPFK_04345"
     CDS             3829..4695
                     /locus_tag="ACZPFK_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1405573.1"
                     /translation="MIIQLLISGIAVGIIYGLIAMGMVLIFRAVGIMNFAQGDFLMFG
                     GYFAYTFNQMLGLNIVLSLVLASLCMGVIGVVFMRTSYWALRKAQTKAIIVSAMGASI
                     VLKEGARLIWGSIPLSLDKVRDGSLALAGATIQWQYVAIIGIALLLMLLVYALLEKTF
                     VGNIMQATAQDQYMSSLVGIPVIVSIGLTFFLSAAITGICGGLLAPIFFLNNTMGATA
                     GSKAFAAIVIGGFGSVPGAIIGGLIVGLVEQFGGAYISSTYQLVLIYVVLIIFLMFRP
                     RGLFKEKIQEKA"
     gene            4708..>5057
                     /locus_tag="ACZPFK_04350"
     CDS             4708..>5057
                     /locus_tag="ACZPFK_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1405574.1"
                     /translation="MLQKKKKSPLALGAAAAFALLLALTPGVTSVYTTHVVSMAMISY
                     LCVLSVYVLLGLCGQNSFAQAGLWGVGAYLAGNVLLRTGGGTLAAFFAAVCGTALVAF
                     VLGFAFFRLKQYYFT"
BASE COUNT          940 a         1581 c         1587 g          949 t
ORIGIN      
        1 ggcgcggatt ggcccgcgcc tgtgcggccc gcctgatcct ggaatgcctg gacagagggc
       61 tctatcccag ctgggacgcc catgacgccc gctcgctcgc cctggccgag agcctgggct
      121 accgcagtgc cggcccttat cccgcctatg agcgcgtcgg gcctgccggc gaataaaaac
      181 cgccccccct tggccttggc tgaggggggc ggtcctttta cagcaagaag aggtcaaaat
      241 caaaggcggc ggggcccttt gcactggtgc gccagccggt gggcaggccg tccggcagag
      301 caaggtccac ctcttccaga ggatggtcca agatcgccgc gttttcaaaa atgcggccgc
      361 actgtgtcga cttggggatc accgcaaagc cgcgctgcag atcccagcgc agggcgatct
      421 gcgccggcgt acagccgtat ttttgtgcca gccgcgcccg cggggcggat actgtgcccc
      481 acatccgcgg ggcccaggcc ctcagggcgg tgcggcgcgc gccgcatcag gcgtaaagcg
      541 ccgtctcgtt gagcagggga tggcgctcca cctggttcac ggcgggcggc acccagtctt
      601 cctccaaaag ccattccagg cggcgaaccg tgcagttgca gacgcccagc gcccgcctct
      661 cctcatagag ccgctccatt gcccgccggg cgtcgagcag ccgtcctcgc tgctgatcgg
      721 gccggtggat gaaaaacagg tccagacggt cgctgcgcag acgggacagg ctctcctcga
      781 aagaggagag gacgcggtca taacgcgtgc tgtagagatc gggcttgccg tcgggggaca
      841 ggtcctcccg gggcgcgccg cagacctgca gcacttctcc ggcggcgctt cgttgccgta
      901 cctctgggcc gtgtcaaagc tgcgatagcc cgcgttcagg gtgacgaccg cgccccgcat
      961 ctcgccgggg ctgtcggtct tgtagacccc gaggcccagc agcggcatcg gtgcgccgtt
     1021 gttcagcagc agggtgcgcg gttgtgccgt cctgtttcgc cctctcgtcc ggcagccccc
     1081 ttgggggcgt ttcttatccc tcgcacggcc gggcggtgcc gttgccgggg atcagggtgg
     1141 tggcaaaacg gatctcccgg ttgagcagcg tgtttttgga ttcgatgcgc tcgatgatca
     1201 gttcggccag gcgcgagccc atggcggcgg ggaactgatc cacgcaggtg ggctgcacat
     1261 agagaaaatc atggttggag atgcggccga agctgctcag cgagagcttg ccgggcacct
     1321 cgacctgcgc ctcgcggcag aactgcagcg cccccagggc cagcaggttg ttcgccgcca
     1381 gcacggcggt ggcgccgcgc tcgtacagcg cccgcgcccc ctccaccccg gaggagatgc
     1441 ggttgaaatt gccgtgatag aggtaagggt aggcgtcgtc caccgtcacc ccaatggtgc
     1501 gcatggcgct ctgaaagccc tccagccgct ctttggcgga ggagacgtag ggctggccgt
     1561 tgatcagccc gatgcggcgg tggcccagtg agatgagctg cttggtcagg tccaccatgc
     1621 cggtaaagtt gtcgttgtcc acaaagtccc ccttgaagcc cgcgtcgggg accttgcgcc
     1681 cgaacagggc gatgggcaaa tgctggctca tgcgggtgat gagctcgttg tttttgccgc
     1741 tgatattgat gatgatgccg tccacctgct tttcacgcag cagggacaaa tagtccagct
     1801 cgcgctgctg gctgctgtcg gtggagcaga caaagaggtt gtagccgcgc tggttgagca
     1861 cgtcctccac agagcgcacc atgctggtaa aaaagtcgtt ggcgatgtcg gagaccacaa
     1921 agcccaccgt cttggagctg tcgtttttca gcgtgcgggc gatggagttg gggtaatagc
     1981 ccagcttctc gatggcgctg ttgacccggt cggccaggtc ctcgcgcacg gtgctgctct
     2041 tgttcagcac ccgggacacg gtggcgatgg ataccccggc ctcccgcgcc acgtccttga
     2101 tcgttgcgtt tgccatgatg agggaaagaa cctccccttt cagaaaaggt ttacaaagca
     2161 atcatacaat aaaccgctct ttcttgcaag cgaaagcggc tgattttgaa aagaaccgca
     2221 gttttgtccc cgttttttcg cagaataaat aaaaacagaa atagaaaaaa gtagagttgc
     2281 acaaaaatta aaacaaattg aaaagagata ttgacaaaaa tgtgtacgcg gagtaggatg
     2341 aaaacgacga gaaaacgtat acagaacgaa aaaacacaaa tcacattttt cacaggccaa
     2401 aacagagagg aaactgtggt ttcgggagct gcaaagcgcc gtttcagcgg tcttgcgctt
     2461 ttttgtttga tgattcagta aacgttttct gaaatacgga acagagcaca atggaggaaa
     2521 cgacatgaaa aagaggattc ttgcaagcct ggcagcggcg accctgatct gcactctcct
     2581 cacgggctgc ggcggcagcg gcgcctcggg cgacgtcatc aagatcggcc tgaccgtgcc
     2641 cctgagcggc gaccgcgcca gcgagggcag ctacgcgtcc aacgccgcag agatcgtgga
     2701 aaaagagatc aacgacgccg gcggcgtgct gggcaaaaat atcaagatcg agatccagga
     2761 ctcgctgggc accgacgtgg gcgccaccaa cgcctacctg aagctggctg cggacaagga
     2821 cgtggtggcc atcatcgggc cggacaactc caacgacaac atcgccatcg ccagctcggc
     2881 ggagtcgtcc cagatcctga ccaccggcca gggctccagc cccacgctgc agacgacctg
     2941 cgaggagggc gagtggatgt tccagctgcg cgcctgcgac tccaccctct gcgccgcgct
     3001 gatggattac gcctacacgg aggtgggcgc caagagcttc accatcatcc acgataccga
     3061 gaccgcctcc gccgaccagg ccaggctgtt tcaagaggcc atcgaggccc tgggcggcac
     3121 cgtggacggc gttgtctcct ataccagcgg caccaaggat ttcaccgccc agctgaccca
     3181 ggcggcggcc aacgactcgg atgcgctggt gatggcctgc ctgtacaccg aggcggcgat
     3241 catcatccag cagacccgcg acatgggcat cgacaagccg gtctttggct ccaacgcgtt
     3301 cggcgacccc atcgccatcg gccttgcggg cgaggcgatc aacgacgtgt acagcgtcac
     3361 cgcctgggtg cccaacaccc ccaaccccct gggcgccgcg ttctcgaaaa agtattccga
     3421 gcagttcaac gaggactgcg ccaaggccgc cgcccagatc cgcgaccatg tctatgtgat
     3481 ctgcgcggcc atcgaaaaag cgggctccac cgaccgggcg gcggtgcggg acgccatgct
     3541 gcagctgacc gattacgagg gcgccatcac cacctataat tgcagccgcc ggggcaactg
     3601 cgggctgggc ggcctgatcg tgcaggtgca ggaccagacc cccaccatca tcaaggagat
     3661 caccaccgag taaacgcgcc gtgggggagc tccccccttg cggcacagac gatccgggcc
     3721 atccgcgcgg gccgtgccat ccggccggcc cgcgttttgc gcccatcgcg ccccgccacc
     3781 ggcgcgggcg gccttgccgc ggcatggaaa ggagtggaag aagcagaaat gatcatacaa
     3841 cttctcatat ccgggatcgc cgtgggcatc atctatggcc tgattgccat gggcatggtg
     3901 ctcatcttcc gggcggtggg cattatgaac ttcgcccagg gcgattttct gatgttcggc
     3961 ggctattttg cctatacgtt caaccagatg ctgggcctca acatcgtgct ctcgctggtg
     4021 ctggcctcgc tctgcatggg ggtcatcggc gtcgtcttta tgcgcacctc ctactgggcg
     4081 ctgcgcaagg cgcagaccaa ggccatcatc gtcagcgcca tgggcgcttc catcgtcctc
     4141 aaggaggggg cccggctcat ctggggctcg atccccctct ctctggacaa ggtcagggac
     4201 ggctcgctcg ccctggccgg ggccaccatc cagtggcagt atgtggcgat catcggcatc
     4261 gccctgctgc tgatgctgct ggtctacgcc ctgctggaaa agacctttgt cggcaacatc
     4321 atgcaggcca cggcccagga ccagtatatg tcctcgctgg tgggcatccc ggtgatcgtc
     4381 tccatcggcc tgactttctt tctcagcgcc gccatcaccg gcatctgcgg cggcctgctg
     4441 gcgccgatct ttttcctcaa caacaccatg ggcgccaccg cgggctcaaa ggcgtttgcc
     4501 gccattgtca tcggcggctt tggcagcgtg ccgggggcga tcatcggcgg gctgatcgtc
     4561 ggcctggtgg agcagttcgg cggcgcttat atctcgtcca cctatcagct ggtgctgatc
     4621 tatgtggtgc tcatcatctt cctgatgttc cgcccgcggg ggctgttcaa agagaagatc
     4681 caggaaaaag cgtaaggggg cagacggatg ttacagaaaa aaaagaagag ccctctcgcc
     4741 ttgggcgcgg cggcggcctt tgcactgctg ctggccctga cgccgggcgt cacctcggtt
     4801 tataccaccc atgtggtcag catggcgatg atctcttacc tctgtgtgct gagcgtttat
     4861 gtgctgctcg gcctttgcgg gcagaactcg ttcgcccagg ccggcctgtg gggcgtgggc
     4921 gcctatctgg cgggtaacgt gctgctccgg accggcggcg gcaccctggc tgcctttttc
     4981 gccgcggtct gcggcaccgc gctggtggcc tttgtgctgg ggtttgcgtt cttccggctc
     5041 aagcagtact atttcac
//
LOCUS       DBMDYA010000126         5043 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4084_length_5043_cov_1.546338, whole genome shotgun sequence.
ACCESSION   DBMDYA010000126 DBMDYA010000000
VERSION     DBMDYA010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_5043_cov_1.546338"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(219..1166)
                     /locus_tag="ACZPFK_04355"
     CDS             complement(219..1166)
                     /locus_tag="ACZPFK_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1405575.1"
                     /translation="MRYKEFGRTGMKVSALTVGTWAIGGANWGDVDREQSIAAIHAML
                     DDGVNFVDTAPVYNAGHSEQVVGEALEGRRSQIFLATKTALYNDPVTGEAIKDGRRDT
                     ILRLCEESLKNLRTDYFDVYILHWPVPGQPVKESMETMMELKAQGVIRHIGVSNYSRE
                     QILEAQQYGEVELIQPPYSMVNRSQEELIQWAADQGLGVMSYASLGAGILTGSIRSMP
                     DFAPTDIRMTFYDFFQEPKFSRTMELLHTLDEVAAAHGAPVPQVALNWSTQKAFIDTA
                     IIGVRNPAEAHENCNGFGWSLTGEEMARIDAAIEATVGR"
     gene            complement(1292..2074)
                     /locus_tag="ACZPFK_04360"
     CDS             complement(1292..2074)
                     /locus_tag="ACZPFK_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1405576.1"
                     /translation="MRLSDNVNGLQHIGYPSNDRDQTVRFLTEKLGFSVVYEAAAGAR
                     FLRRGDLCLEVYPVEEPPFTVDGGRHDELAVTVDHLEDAAARAGFGGPVEEQLLPNGQ
                     LLRSVRGKGPDGEGLCLNELSGASAHPLATFSYLRIPVADLRVTTAFYEELGFEFLFY
                     LQEKDGGGRCDAACLALGDWIIKLVQREGWGPRSARDSHLDHMALDIRCIGQAFASAK
                     AFQLELLHDQPQFLPFWQYGVRFFTLRGPDGQKVELSEMLRV"
     gene            complement(2088..3047)
                     /locus_tag="ACZPFK_04365"
     CDS             complement(2088..3047)
                     /locus_tag="ACZPFK_04365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405577.1"
                     /translation="MIDVAAIGELLIDFTPGDGEWSFVAHPGGSVANFLAQLSVLGKS
                     TAFMGMVGQDQFGAYLRDTLVGVGVGTEGLKMNPDVGTTLAFVHINAQGDRSFTFYRR
                     PGADMMFTEQDIDYGLIDSARYFHFAGVSLTKEPMRSATFAAASYARRAGKVISFDPN
                     YRATLWDSEQEAAEVLRLGVPLCDLLKVSDEEALMITGEATVQKAAAALAAMGPALVL
                     VTLGARGCHCYHRNLDRHFDTYDTKVVDTTGSGDSFLGAVVYRLSCLGLPVAALSEAQ
                     LAEMVDFGNAAGSCCAAGYGAIPSLQDEQGIRACMAGTPKLKL"
     gene            complement(3089..3925)
                     /locus_tag="ACZPFK_04370"
     CDS             complement(3089..3925)
                     /locus_tag="ACZPFK_04370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405578.1"
                     /translation="MAIIESKFFSKELFRSVCVNVILPLPESGDAFFGSKTLYPAPGQ
                     KYPVLYLLHGFSADHSDWARFSGIERYAQERQIAVVMPGVDNSFYCNLPEGGNYYDYY
                     TRELPQVMEALFPISGKRENKFLAGLSMGGYGAFKAALREPDQYAAAVSLSGGMSCGA
                     GEGWACTIGSASPDEVPTGRFSRAAFGEHGEHLKPEENDLKVLLRAAMASGKPMPLLY
                     QACGTEDFVYPCNAEYRDFARELGAPLTYEEGPGIHNWDFWDPYIRRAVEWLPTKGGF
                     TD"
     gene            complement(3941..4765)
                     /locus_tag="ACZPFK_04375"
     CDS             complement(3941..4765)
                     /locus_tag="ACZPFK_04375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405579.1"
                     /translation="MAIFECKMFSKELFSSVSVNVILPTPESGDYFFGSRTYYPKAGQ
                     KYQTLYLLHGFTADFSDWQRFSSIERYAQAHQLAVVMPSVNNSSYCNLRNGGNYYNYY
                     LEELPAQMRAIFPLSTKRENNFVAGLSMGGFGALKAAFTRPEMFAAAASLSGGAASRR
                     GDGKKADGGSPIQTDKWRAASYGPNGEYFNPDADDIWVASQKLVESGRTAPRLYIACG
                     TEDFTYQGNLELQRYLKGLGLDVTYEEGPGRHDMAFWDKYIERVIEWLPLTGGFVD"
BASE COUNT          883 a         1631 c         1597 g          932 t
ORIGIN      
        1 gcagcatccg ggagctgcgc ctttgaggcg aggcgggctt tccggcccgt cggccgtcgg
       61 ccagaggggc cgttggcctt accgcgacgg ccggaggccg gggcccgacc gcagcccccg
      121 ctcgttcttt ccgccgtggt acggacggga ttcagaaagc gcaggcgaat ccgcgctcac
      181 ggcctccaca gcctttcctg ccgtggcgcg ggcggtgctc agcggcccac ggtggcctcg
      241 atggccgcgt cgatgcgggc catttcctcc ccggtgaggc tccacccaaa gccgttgcag
      301 ttctcatgcg cctcggcggg gttgcgcacg ccgatgatgg cggtgtcgat gaaagccttt
      361 tgggtggacc agttgagcgc cacctgcggc accggcgcgc cgtgggcggc cgccacctcg
      421 tccagggtgt gcagcagctc catggtgcgg gagaattttg gctcctgaaa gaaatcgtaa
      481 aaggtcatgc ggatgtcggt gggggcaaaa tcgggcatcg agcggatgct gccggtgagg
      541 atgccggcgc ccagcgaggc gtaggacatc acgcccagcc cctggtccgc ggcccactgg
      601 atcagctcct cctgagagcg gttgaccatc gagtaagggg gctggatcag ctccacctcc
      661 ccgtactgct gcgcctccag gatctgctcg cgggagtagt tggagacccc gatgtggcgg
      721 atcacgccct gggccttgag ctccatcatc gtctccatgc tctctttcac cggctgcccc
      781 ggcaccggcc agtgcaggat atagacgtcg aagtaatcgg tgcgcaggtt ttttagcgac
      841 tcctcacaga gccgcaggat ggtgtcgcgc cgcccgtcct tgatggcttc gccggtgacc
      901 gggtcgttgt aaagcgcggt cttggtggcg agaaagatct ggctgcgccg cccctccagc
      961 gcttcgccca ccacctgttc ggaatggccc gcgttgtaga ccggcgcggt gtccacaaag
     1021 ttcaccccgt cgtccagcat ggcatggatg gcggcgatgc tctgctcccg gtccacatcg
     1081 ccccagttgg cgcccccgat ggcccaggtg cccaccgtga gagcggatac tttcatcccg
     1141 gtcctgccaa attccttgta acgcacaatg atcgctcctt tctaattgga aaacaagatt
     1201 aaattaaatt aaaagctcct aagaatcccc tgcccggctt tttgcttggg cgggggatct
     1261 tgtattccgg cgttggggcg gcaaagagcg ctcagacgcg cagcatctcg ctcaactcca
     1321 ctttctggcc gtccggccca cgcagcgtga aaaagcgcac gccgtactgc cagaacggca
     1381 aaaactgcgg ctggtcgtgc agcagctcca gctgaaaggc tttggccgag gcaaaggcct
     1441 gaccgatgca gcgaatgtcc agggccatgt ggtccaaatg gctgtcccgg gcggagcggg
     1501 ggccccagcc ctcccgctgg accagcttga tgatccagtc gcccagcgca agacaggcgg
     1561 cgtcgcagcg gccgcccccg tctttttcct gcaggtagaa gagaaactcg aacccgagct
     1621 cttcataaaa ggcggtggtg acccgcagat cggccaccgg tatgcgcaga tagctgaagg
     1681 tggccagcgg atgggccgat gcgcctgaga gctcgttgag gcaaagcccc tcgccgtccg
     1741 gccccttgcc gcgcacgctg cgcagcagct ggccgttggg cagcagctgt tcttccaccg
     1801 ggccgccgaa acccgcccgg gcggccgcgt cctccagatg atccacggtg acggccagct
     1861 cgtcgtggcg tccgccgtcc accgtgaacg ggggctcctc caccgggtag acctccaggc
     1921 aaagatcgcc gcggcgcaga aagcgggccc cggccgccgc ctcgtagacc accgaaaagc
     1981 ccagcttttc ggtgaggaac cgcacggtct gatcccggtc gttggacggg tagccgatgt
     2041 gctgcaggcc gtttacattg tcagacaaac gcatgggatg ctctccttta cagtttcagt
     2101 ttcggcgttc cggccatgca ggcgcggatg ccctgttcgt cctgcaggga ggggatggcc
     2161 ccgtaaccgg cggcgcagca ggagcccgcc gcgttgccga agtccaccat ctccgccagc
     2221 tgcgcttcgc tgagggccgc caccggcagg cccaggcagc tcaggcgata gaccaccgcg
     2281 cccaggaaag aatcccccga gccggtggtg tctaccacct tggtgtcgta ggtgtcgaaa
     2341 tgccggtcca gatttctgtg atagcagtgg cagccgcgcg cgcccaacgt caccaggacc
     2401 agcgccgggc ccatggccgc cagcgccgca gccgcctttt gcacggtggc ttcgccggtg
     2461 atcatgagcg cctcctcgtc ggacaccttg agcaggtcac agaggggcac gcccaggcgc
     2521 agcacctccg ccgcctcttg ctcgctgtcc cacagggtgg cgcggtagtt gggatcgaac
     2581 gagatcacct tgcccgcccg gcgggcatag gacgcagcgg caaaggtggc gctgcgcatc
     2641 ggctccttgg tcagcgagac gccggcaaag tggaaatagc gggcgctgtc gatcaggccg
     2701 tagtcgatgt cctgctcggt gaacatcatg tcggcgccgg ggcggcggta aaaggtgaag
     2761 ctgcggtcgc cctgggcatt gatgtgcaca aaggccaggg tggtgccgac gtccgggttc
     2821 atcttcagcc cctcggtgcc gaccccgacc cccactagcg tgtcgcgcag gtaggcgccg
     2881 aactgatcct ggccgaccat ccccataaag gcggtcgatt tgcccaggac cgagagctgg
     2941 gccaaaaagt tggccacgct gccgccgggg tgggcgacga agctccactc gccgtcgccc
     3001 ggtgtgaagt cgatcagtaa ttctccaatt gcagctacgt caatcatctt gttttcctca
     3061 ttccattggg tatggcgctg tctgccgctc agtcggtaaa gccgcctttt gtgggcagcc
     3121 attccacggc gcgccggata tagggatccc agaaatccca gttgtggatg ccggggccct
     3181 cctcgtaggt cagcggcgcg cccagttccc tcgcaaagtc gcggtactcc gcgttgcagg
     3241 gatagacaaa atcctcagtg ccgcaggcct gatagagcag cggcatgggc ttgccgcttg
     3301 ccatggccgc ccgcagcaaa actttcaggt cgttttcctc cggcttcaga tgctctccgt
     3361 gctcgccgaa agcggcgcgg gagaaacggc cagtgggcac ctcgtcgggg ctggctgagc
     3421 cgatggtgca ggcccagccc tcgccggcgc cgcagctcat gccgccggag aggctgactg
     3481 ccgccgcgta ctgatccggc tcacgcagcg ccgccttgaa agcgccgtat ccccccatcg
     3541 agagcccggc cagaaacttg ttctcccgct tgccggagat ggggaacagg gcctccatca
     3601 cctggggcag ctcccgcgta taatagtcgt aatagttgcc cccctcgggc aggttgcagt
     3661 aaaagctgtt gtccaccccc ggcatcacca ccgcgatctg ccgctcctgc gcgtaccgct
     3721 cgatgccgga aaagcgcgcc cagtcggagt ggtcggcgga aaagccgtgc agcaggtaga
     3781 gcaccgggta cttctgcccc ggggcggggt agagggtctt ggagccgaaa aaggcgtccc
     3841 ccgactccgg caggggcagg atcacgttca cgcagacgct gcggaacagc tcttttgaaa
     3901 aaaacttgct ctcaatgatc gccatatcgc atcccctcct ttagtccacg aatccgccgg
     3961 tgagcggcag ccattcgatg accctctcga tgtacttatc ccagaacgcc atatcgtggc
     4021 gtcccggccc ttcttcatag gtcacgtcca ggcccaggcc tttcaggtac cgctgaagct
     4081 ccagattgcc ctgataggta aaatcctcgg tgccgcaggc gatgtagagc ctgggggccg
     4141 tccgtccgct ctccaccagt ttttgggagg ccacccagat gtcgtcggcg tccgggttaa
     4201 aatactcccc gttcgggccg taagaggcgg cccgccactt atcggtctgg atgggggagc
     4261 cgccgtccgc cttttttcca tccccccgcc ggctggccgc gccgcccgaa aggctcgccg
     4321 cggccgcaaa catctccggt ctggtaaagg cggctttcag ggcgccgaaa ccgcccatgg
     4381 acagccccgc tacaaaattg ttctcccgtt tggtggacag ggggaagatg gcccgcatct
     4441 gggcgggcag ctcctcgaga taataattgt aatagttgcc gccgttgcgc aggttgcagt
     4501 agctgctgtt gttgacgctg ggcatcacta ccgccagctg atgggcctgc gcgtaccgct
     4561 cgatgctgga aaagcgctgc cagtcgctga agtcggcggt gaagccgtgc agcagataca
     4621 gggtctggta cttttgaccc gccttgggat aataggtcct ggagccgaag aagtaatcgc
     4681 ccgactccgg cgtgggcagg atcacattga cgctgacgct ggaaaaaagc tcctttgaga
     4741 acatcttaca ctcaaaaatg gccataaatc aataccgtcc ctttccgggg cccctgcccg
     4801 gcggcgggag gggcactttt tccccttttc cgccgtgccg gccccttttc tgaaaaccgg
     4861 gtgttcacgg gatgatgaga atcttgccgt ggctgcggta gctgtcgtcg gaaaagacct
     4921 tttccgcctc ggccaggggc acccggtcgg tgatgacggt gtccaggtcc agcctgccgg
     4981 aggccagcag gtcgatgacg cgctggctga ccagggggtt gatgtaagag ccggtgatgg
     5041 tga
//
LOCUS       DBMDYA010000127         4993 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4130_length_4993_cov_1.411693, whole genome shotgun sequence.
ACCESSION   DBMDYA010000127 DBMDYA010000000
VERSION     DBMDYA010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_4993_cov_1.411693"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="ACZPFK_04380"
     CDS             <1..222
                     /locus_tag="ACZPFK_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016154.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HIR1405580.1"
                     /translation="EVTRGDLHLPANGPLKPSMPLVVDGALNDDAAEYCGVDRAWLEK
                     TLRAERLGPADVLLMLCNDAHEYTIVKKQ"
     gene            284..652
                     /locus_tag="ACZPFK_04385"
     CDS             284..652
                     /locus_tag="ACZPFK_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HIR1405581.1"
                     /translation="MRRFKIACALLTAILLISLAGCLSVDRITARMSSYLDTAEQQNQ
                     DGDYPALADTLKELDDYYASKEWVLALFLRRDYATSALLGFSTLKSFDSEEYKTDFDA
                     ELERARTQIAVTRHLFLSFF"
     gene            complement(628..1104)
                     /gene="nrdR"
                     /locus_tag="ACZPFK_04390"
     CDS             complement(628..1104)
                     /gene="nrdR"
                     /locus_tag="ACZPFK_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR1405582.1"
                     /translation="MKCPYCGEVEPKVIDSRPADDGERIRRRRECLACQKRFTTYEIV
                     ETVPLMVVKKDKSREAFDRQKLLNGLMRACEKRPVAIGTLERAVDEIEQSLLNTLDRE
                     VTSLEIGELAMAQLKNIDEVAYVRFASVYRQFSDINTFMDELKELLSSNQKKLKNK"
     gene            complement(1121..1915)
                     /locus_tag="ACZPFK_04395"
     CDS             complement(1121..1915)
                     /locus_tag="ACZPFK_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1405583.1"
                     /translation="MRVGVSSSCFYPDCTEDAVRRLQEAGIEEIELFLNTFSELEPGY
                     TAALRRQLAAQGTRVLSFHPFTSGMEPFFFATDYGRRAADGRELYKRLFDCAAQYGAR
                     VFVFHGDYKTTPYPFERYCESFAGLSSLAREYGLLLCQENVVRCKCGSVDSVRRMREL
                     LGREAHFALDLKQALRSGADIYDMLDAMGEGLAHIHISDHAPGRDCLAPGCGDFDFRA
                     FFQRLRRIPYQGDVVVELYRSNFRNAEELAGSAAFLRELSAEQAVE"
     gene            complement(1971..3236)
                     /locus_tag="ACZPFK_04400"
     CDS             complement(1971..3236)
                     /locus_tag="ACZPFK_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1405584.1"
                     /translation="MADYLAMGKAELEKEKAALEKSYQEYREKGLKLDMSRGKPCPEQ
                     LDLSTGLLGITDTRTEEGMDVRNYGCLEGIPEARRLFAELLGADARQVLVGGNSSLNL
                     MFDVIGAGCRDGLGGAPWDACEHKKFLCPSPGYDRHFRVTEYYGFELITVPMLESGPD
                     MDRVEELVRDPQVKGIWCVPTYSNPDGYCYSDETVRRLAALKPAAENFKIMWDNAYVV
                     HYLGDGPQQTLNILEECARCGNGELPVMFCSTSKITFPGAGVAALAAGPKTFAALTGA
                     LFPMTIGFDKINQLRHVKFLRDKAHILAHMEKHKAIIKPKFDAMQRLLAEGLSGCGQI
                     ARWTHPQGGYFISLYTLDGCAKRTIALCREAGVVLTGAGAAFPYGRDPHDSNIRLAPT
                     YPAQAEVEEATRLLCVALRLATVEHLLAR"
     gene            3590..3680
                     /locus_tag="ACZPFK_04405"
     tRNA            3590..3680
                     /locus_tag="ACZPFK_04405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3625..3627,aa:Ser,seq:gga)
     gene            complement(4048..4428)
                     /locus_tag="ACZPFK_04410"
     CDS             complement(4048..4428)
                     /locus_tag="ACZPFK_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405585.1"
                     /translation="MKKILPFAAALLLAVSLSGCGEPDFDGSRLGNDSQLVMEYKTLN
                     GTDSQELELKEGDLLEIQLLNHGGGSLKAVVQKGEEEPIFEEEDISVNRVTLEIEESG
                     TYTVTVTGEKARGSVSFVKTEKEG"
     gene            complement(4668..>4993)
                     /locus_tag="ACZPFK_04415"
     CDS             complement(4668..>4993)
                     /locus_tag="ACZPFK_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563970.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HIR1405586.1"
                     /translation="ERYKMVTKEFKGLVHGDYGKTPAPIKPEFVKQILGDEQPITCRF
                     ADTLEPEVEKLKAEAAKWSIQEEDVLTYAMFPQVAPKFFETRNQKRVGIDGAHVDMAD
                     KSHPV"
BASE COUNT          948 a         1628 c         1393 g         1024 t
ORIGIN      
        1 gaggtgacgc ggggcgacct gcatctgccg gccaacggcc ccctcaagcc ctcgatgccg
       61 ctggtggtgg acggcgctct caacgacgac gccgccgagt actgcggcgt ggaccgcgcc
      121 tggctggaaa agacgctgcg cgccgaacgg ctggggcccg ccgacgtgct gctgatgctc
      181 tgcaacgacg cgcacgagta caccatcgtc aaaaagcagt gagccgcggc cgcggccacg
      241 gccccgcccc cagtcaaaac catatgggaa aggaggcgcg tccatgcgcc ggttcaagat
      301 cgcttgtgcg ctgctcacag ccatcctgct gatctcgctt gcgggctgcc tctcggtgga
      361 ccgcatcacc gcccgcatgt cctcttatct ggacacggcg gaacaacaaa accaggacgg
      421 ggactacccc gccctggccg atacgctcaa ggaactggac gactactatg cctcaaaaga
      481 atgggtgctg gccctgtttt tgcgccggga ctacgccacc agcgcgctgc tgggcttttc
      541 caccctgaag agctttgaca gcgaggaata caagaccgat ttcgacgccg agctggagcg
      601 cgcccgcacc cagatcgcgg tgacccgtca cttgtttttg agctttttct gattggagct
      661 gagcagctct ttcagctcat ccataaaggt gttgatgtcg ctgaactggc gatagaccga
      721 cgcgaaccgc acataggcca cctcgtcgat gtttttgagc tgcgccattg ccagctcgcc
      781 gatctccagc gaggtgacct cccggtccag ggtgttgagg agggattgct cgatctcgtc
      841 caccgcccgc tccagcgtgc cgatggccac cggccgcttt tcgcaggcgc gcatcaagcc
      901 gttgagcagc ttctggcggt cgaacgcctc ccgtgatttg tcttttttga ccaccatcag
      961 cggcacagtc tccacgatct cgtaggtggt gaaccgcttc tggcaggcga ggcattcccg
     1021 gcggcggcgg atgcgttccc cgtcgtctgc agggcgcgag tcgatcacct tgggctccac
     1081 ctctccgcaa tacggacact tcataaacgt tcctcctatc ctattccacc gcctgctctg
     1141 cagacagctc tctcaaaaaa gccgcgctgc ccgccagctc ctccgcgttg cggaagttgc
     1201 tgcggtacag ctccaccacc acgtcgccct gataggggat gcggcgcagc ctctggaaaa
     1261 aagcgcggaa atcaaaatcg ccgcagccgg gcgcaagaca gtccctgccg ggggcatggt
     1321 cgctgatgtg gatatgcgcc aggccctccc ccatcgcatc cagcatgtca tagatgtctg
     1381 cgccgctgcg cagcgcctgc ttgaggtcca gcgcaaagtg cgcctcccgc cccagcagct
     1441 cgcgcatgcg gcgcacgctg tccacactgc cgcacttgca gcgcaccaca ttttcctggc
     1501 agagcaaaag accgtactcc cgcgcaaggg aggaaagccc ggcaaagctt tcacaatacc
     1561 gctcaaaagg atagggggtg gtcttgtaat cgccgtgaaa gacaaagacg cgggccccat
     1621 actgcgccgc gcagtcgaac aggcgcttgt agagctcgcg gccgtccgcc gcgcgccgcc
     1681 cgtaatcggt ggcgaaaaag aaaggctcca taccggaggt aaagggatgg aacgagagca
     1741 cgcgggtgcc ctgcgccgcc aattgccgcc gcagggcggc tgtataaccg ggttccagct
     1801 cgctgaacgt gttcaaaaac agctcgatct cttcgatccc cgcctcctgc agccgccgca
     1861 cggcgtcctc ggtgcagtcg ggataaaagc aagagctgga aacacccacg cgcaccccgg
     1921 cctctcccct ttcacctgaa atttctgccc ggacaccgcc gggccgcccg tcaccgggcc
     1981 agcagatgtt ccaccgttgc cagccgcagc gccacgcaca gcagccgggt tgcctcctcc
     2041 acctccgcct gggccggata ggtgggcgcg aggcggatgt tggagtcgtg cgggtccctg
     2101 ccataaggaa aagccgcgcc cgcgccggtg agcaccaccc ccgcctcccg gcagagggcg
     2161 atggtgcgct ttgcgcaccc gtccagcgtg tagaggctga tgaaatagcc tccctgggga
     2221 tgggtccagc gtgcgatctg cccacagccg gacaggccct cggccaaaag ccgctgcatg
     2281 gcgtcgaact tcggtttgat gatggcctta tgcttttcca tatgggcgag gatatgcgcc
     2341 ttatctctca aaaacttcac gtggcgcagc tgattgatct tgtcaaagcc gatggtcatg
     2401 gggaacagcg ccccggtgag ggcggcaaag gtcttcggcc cggccgccag cgcagcgacc
     2461 cccgcgccgg ggaacgtgat cttgctggtg gagcagaaca tcaccggcag ctcgccgttg
     2521 ccgcagcggg cgcactcctc taaaatattc agtgtttgtt gcgggccgtc gcccagatag
     2581 tggaccacat aggcgttgtc ccacatgatc ttaaagttct cggccgcggg cttcagcgcc
     2641 gccaggcgcc gcaccgtctc gtcggaatag cagtagccgt ccgggttcga ataggtgggc
     2701 acgcaccaga tgcccttgac ctgcgggtcg cgcaccagct cctccacccg gtccatatcg
     2761 gggccgcttt ccagcatggg cacggtgatc agctcaaagc cgtaatactc ggtgacccgg
     2821 aaatggcggt catagcccgg cgaggggcag aggaactttt tgtgttcgca ggcgtcccag
     2881 ggcgcgccgc cgaggccgtc ccggcagccg gcgccgatca cgtcgaacat caggttgagg
     2941 ctggagttcc cccccaccag cacctgacgc gcatcggcgc ccagcagctc ggcaaacagg
     3001 cgcctcgcct ccgggatgcc ctccaggcag ccgtaattgc gcacgtccat gccctcttcc
     3061 gtccgggtgt cggtgatgcc cagcagcccg gtggacaggt ccagctgctc ggggcagggc
     3121 ttgccgcggg acatatccag cttgagcccc ttttccctgt actcctggta gcttttttcc
     3181 agagctgctt tttctttttc cagctctgcc tttcccatcg cgagataatc cgccatttcg
     3241 tttccccctg taaaaagtga taaaatatcg tcacagccct gcaccgcttg ggaggaaagt
     3301 ccctcccaat tcccgtgcgg cgcaaagccg cgtacggctt tcattatagc ctttttttac
     3361 ctgttccgca ataaaaacgg cctatttccc ttttgggacg ccttttccat ccacctgccc
     3421 ttggtagcgc ggcgacgggg cccctccgta tttttcagcg ccgcgtcgaa gattttctct
     3481 tgacaacacg cggtaaattg attattatat aaaagcgccc tttgagcgcg gacagacttt
     3541 gcaaaaagtg aatcaggcaa aaaaagttga caagcccgtt tcgtcgcatg gagagatggc
     3601 tgagctggtc taaggcgcac gattggaaat cgtgtatact agaaatagta tcaagggttc
     3661 gaatcccttt ctctccgcca cagggatacc accgcaaaag cggtggtatt tttttgttcc
     3721 caccatccca cgcaaaggcc cgacggccag cgagaagcat ttgttttttc agtcgttccc
     3781 gtattttgcc gctctcgccg ggctaccgct tccggccgca gggtgcgaaa aaagggccgg
     3841 ccgttttctc gcagccgcgc ccttatcata tgcccctgtg ccgccgcccg gttcctcccc
     3901 gccgggtatg cataacgaaa tgataccggg aaaggcccgc agagtacccc ctgccggagt
     3961 gctctgcggg ctttcttttt ctcgcgacac cgcggcagcc gcgccgcggc gctcatctgc
     4021 gaaaagcggc cgcgcgggac gcttttttca gccctctttt tctgttttta caaagctgac
     4081 gcttccccgc gccttttccc ccgtcaccgt cacggtatag gtgccgctct cctcgatctc
     4141 cagcgtgacc ctgttgaccg agatatcctc ctcttcaaaa atgggttcct cctccccctt
     4201 ttgaaccacg gctttgagag agccgcctcc gtggttgagc agctgtatct ccagcaggtc
     4261 cccctctttc agctcaagct cttgcgagtc ggtcccgttg agcgttttgt actccataac
     4321 caactggctg tcgttgccca atcggctgcc gtcaaaatcc ggttccccac agccggagag
     4381 agagacggcc aacagcaggg cggcggcaaa gggcaaaatt tttttcaaaa cgacccctcc
     4441 ttttttcttg aatcaagtct tctgctacct ataaaactgt ttagggacgg taatttcttc
     4501 aatctgcgac catattgttt aaaatgggca aaatccgctg cctctggaag caaaacgtcc
     4561 ccaaaaggga aagtggcctc cccaggacgg aaaagcccgt cgggagacca ctttcaaaac
     4621 ttcgcttctc tttttatgct tttagaaccc acacttaaac aatttgttta gactggatgc
     4681 gacttgtctg ccatatcgac gtgggcgccg tcgatgccga cgcgcttctg attgcgggtc
     4741 tcaaagaact tcggcgcgac ctgcgggaac atggcgtagg tcagcacatc ctcctcctgg
     4801 atcgaccact tggcggcctc agccttcagc ttctccacct cgggctccag cgtgtccgca
     4861 aagcggcagg tgatgggctg ctcgtcgccc agaatctgct tgacgaactc gggcttgatc
     4921 ggcgccgggg tcttgccgta atcgccgtgc accaggccct tgaactcctt ggtgaccatc
     4981 ttgtagcgct cgc
//
LOCUS       DBMDYA010000128         4978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4144_length_4978_cov_1.572073, whole genome shotgun sequence.
ACCESSION   DBMDYA010000128 DBMDYA010000000
VERSION     DBMDYA010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_4978_cov_1.572073"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="ACZPFK_04420"
     CDS             <1..547
                     /locus_tag="ACZPFK_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405587.1"
                     /translation="SALLERHGLAQGPNDLDLVAAESDFEALRRALSALGSERPRPPK
                     PPFISRAMTGFEIEGEELDLIAGYGTRCEEGDYFLPFDRESIAEAVEIEGRLIPFMAL
                     EDWLVLYGLMPAAKRQLKCVKVARSLAQSGVGHPALLARALRQPLPQGLRRLCRALLE
                     GTAEDARLEQRQRALEAELGL"
     gene            891..1700
                     /locus_tag="ACZPFK_04425"
     CDS             891..1700
                     /locus_tag="ACZPFK_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1405588.1"
                     /translation="MYRIGEFSKITSLTVKALRYYDDQGLLRPSARDESGYRLYSEED
                     YQTARRIALLRRFGFSIAELRDVLRGSSSDEELAVYLREKQRLLEQEMRRKQAQMNAL
                     EDAIASFSREQEEAEAMETEQKELPAQWVLSARFRGAYPEVSRHIGALYKAAKGGASG
                     PPFSLYYDDEYRDGDADIELCLPVREGTHGAKRLAGARVLSLLYIGPYDSLGPAYKQL
                     LDTACAQGLACLLPSREIYHKGPGMIFKGNAAAYRTEIAVPVRPAKGDGTR"
     gene            1697..2356
                     /locus_tag="ACZPFK_04430"
     CDS             1697..2356
                     /locus_tag="ACZPFK_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR1405589.1"
                     /translation="MSEKLDYKKAFPDLYAPKQAPALVEVPPMTFIMVDGKGDPNEEG
                     GSYQQAVGLLYGLTFAIKMSRKSGDAPEGYFEYVVPPLEGLWWSEDDLWRGESAPDKS
                     RFCWTSMIRQPEFVTPQVFEWAKGALSKKKPELDLSRARLETFCEGLCVQAMHVGPYD
                     AEPETLARMQRFALDSGCREELENGRRHHELYLGDPRRTAPQRLRTVLRHPVKRAGLS
                     S"
     gene            3280..>4978
                     /locus_tag="ACZPFK_04435"
     CDS             3280..>4978
                     /locus_tag="ACZPFK_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1405590.1"
                     /translation="MRVKNDLDRRKLVAAAMGQVPCDLTVKNVRFVNVITGEVYPAEV
                     DVLDGVVVRVREKERQAELESRTVYDGGGRYLAPGCIDIHMHVESTMMTPENFGKAAV
                     LCGTTSVFVDPHEIANVLGIDGVRFMIDNAKASPVRQFNLAPSCLPSVPGAEGNGATF
                     GPQEIAQLLDTEGVFGVAELMDFYNVTHGSEKMRGIAAEGLARDMLIQGHAPKLAGGA
                     IAAYALAGPSDNHSIRDSWEVVENLHAGLYVDLQENSLGASTMPQLVEGLKGMRYTDH
                     VCLCNDDVHAKDLLETGHVNRLIKSVLRQGVHPLDALRWSTYNSARSARIDDLGALAP
                     GYVADMQLLDELDGRDPYAVFFEGALVAEGGKLVEEKPAQPIPAAVRGGTVRVAGLTG
                     PEALALRAPAGAGETVRVAVLDYDTPTARSDLFYEELPVKDGLVDLSGRDDLCFIAVW
                     NRHGSGEGTVSVARHLYLREGAMATTVSHDCHNLTLCYKRPQDGYAAVKALVGCGGGF
                     CTVKDGQVTGLLELPVAGLMSELPCRELVPAIARQEQATDAIFERPGRMMKLSLGDGR
                     "
BASE COUNT          855 a         1550 c         1762 g          811 t
ORIGIN      
        1 cagcgcgctg ctggagcggc acggcctggc acaggggccg aacgatttgg acctggtggc
       61 tgcggagtcg gattttgagg cgctgcgccg cgcgctgagc gcgctgggca gcgagcggcc
      121 gcgaccgccc aaaccgccgt tcatcagccg cgccatgacg ggcttcgaga tcgaggggga
      181 agagctggac ctcatcgcgg gctatggcac ccgctgtgag gaaggggatt attttctccc
      241 ctttgaccgg gagagcatag cggaggccgt ggagatagag gggcgtctca tcccatttat
      301 ggcgttggag gactggctgg tgctgtacgg gctgatgccc gcggccaaac ggcagctcaa
      361 gtgtgtcaaa gtcgcccgca gcctggcgca aagcggcgtc ggccacccgg cgttgttggc
      421 ccgtgcactg cggcagccgt tgccgcaggg gctgcgtcgg ctctgccggg ccctgttgga
      481 gggcacggcg gaggacgctc ggctggaaca gcgtcagcgg gcgctggaag cagagctggg
      541 gctttgaaat tgcttttttc atctgcatcc ggtcgccctt gggccgcagc tgtcacggca
      601 agctgttttc ggcccattcg tctcgcttgc cgggcccacg gccgctttgt ggcggcccct
      661 ctgaaaaaga cgccgtgatg tgcgccgttt tagcaaggta cggatcggtt taaatggtct
      721 tttgtgcgcg ccctctcctt tcgcgagtga acgcttaaaa cgttaaaacc gtgtccaaaa
      781 cggcctgcgg ggctgctttt ataattttgc cgtacaaagc cgcaaaaaac gcttgactct
      841 cctctgcggg ggagggttta tgctgtttac aggaaaggag gcgcgggcgg atgtatcgga
      901 tcggggagtt ttcaaagatt accagcctga ccgtcaaggc gctgcgctat tatgacgacc
      961 aggggctgct gcggccctcg gcgcgggatg agagcggcta ccggctctac agcgaggagg
     1021 actaccagac ggcgcggcgc atcgcgctgc tgcgccggtt cggcttttcc atcgccgagc
     1081 tgcgggacgt gctgcgcggc agcagcagcg acgaggagct ggcggtctac ctgcgggaaa
     1141 agcagcggct gttggagcag gagatgcgca ggaaacaggc gcagatgaac gcgctggagg
     1201 acgccatcgc ctctttttcc agagaacagg aggaggccga agcgatggag acggaacaaa
     1261 aagaactgcc ggcgcagtgg gtgctgagcg cccgcttccg cggggcctat cccgaggtga
     1321 gccgccacat cggggcgctg tacaaggcgg cgaagggggg cgcgtccggt ccgcctttca
     1381 gcctgtatta cgacgatgaa taccgggacg gggacgcgga tatcgagctc tgcctgccgg
     1441 tgcgcgaggg cacgcacggg gcaaagcggc tggcgggggc cagggtgctc agcctgctgt
     1501 atatcggccc ttacgacagc ctcggcccag cctacaaaca gctgctggac acagcctgcg
     1561 cgcagggcct ggcctgcctg ctgccctcgc gggagatcta tcacaagggg ccgggcatga
     1621 ttttcaaagg caatgcggcc gcctaccgca ccgagatcgc ggtgccggtg cggccggcga
     1681 aaggagacgg gacgagatga gcgaaaagct ggactacaaa aaggcatttc ccgatctgta
     1741 tgcccccaaa caggccccgg cgctggtgga agtgccgccc atgaccttta tcatggtgga
     1801 cggcaaaggg gaccccaatg aggagggcgg cagctatcag caggcggtgg gcctgcttta
     1861 cggcctcacc tttgccatca agatgagccg caaaagcggc gacgcgcccg agggctattt
     1921 tgagtatgtg gtgccgccgc tggaggggct gtggtggagc gaggacgatc tctggcgcgg
     1981 ggagagcgcg cccgacaagt cgcgtttctg ctggacctcc atgatccgcc aaccggagtt
     2041 cgtcacgccg caggtgttcg aatgggccaa gggggcgctg tcaaaaaaga agccggagct
     2101 ggatctctcc cgcgcccggc tggagacctt ttgcgagggc ctttgcgtgc aggcgatgca
     2161 tgtgggcccc tatgacgccg agcccgagac gctggcgcgg atgcagaggt tcgcgctgga
     2221 cagcggctgc cgcgaggagc tggaaaacgg gcggcgccat cacgagctgt acctgggcga
     2281 cccgcgccgc accgcgcccc agcggctgcg caccgtgctg cgtcacccgg tgaagcgcgc
     2341 gggcctttcc agctgagggc cggggccgac aaaagcggat acccaataca gggaaaaagc
     2401 aaagagaacg cctgccacgg gtcagacctg cgggcaggac gttctttttg cttttctttg
     2461 taacagcgcc aaaacggctc gcgggggcgg cagatccgcc cccgcccggc cgcggggcgc
     2521 tcagccgcag gaggacccga ccgtctcccc atgagagccg cccgcatcca gccgtggggc
     2581 ggcccgccgc ggaggttctc ttttgcgcgc cggattctcc ggcttcgcac cccggccgcg
     2641 gccaacccga tttccggctc ccctttgaaa agttgttatg tgggggatgg aaaggaagac
     2701 cgctgggccg gtgcggccgc tcgcccgggc cgcgcccctg gcctcctttc ccggcgcgga
     2761 ttctatctcc cccttgcccg gggaggcgcg ggagaggcgc cttgggcgcg catggagttg
     2821 cgcgggatgg agaaaaacgg gattttccac aaggcctttt cccgctttgc ccaaaacagc
     2881 gcatggctcc gctttcagag aggagggtca cccgaacggg gcgcggctga gccgtcccgg
     2941 ggcgagaacg gcccggggcc ggcagtcccg tccgcctccg ctgcggtgct cttatcctgc
     3001 cagccccttt ccgccgctgc acggcggccc agacggccgc aggccctgcg agcgcgccga
     3061 gctgtttgcc ctgctgcgac ggtccgacag cccgggcggc gcggatttcc cttgcaggcg
     3121 cagcgcgcgg ccgggcgggg tggggagaga accggcctcg ggcggcccgc ttttacaaag
     3181 ccgtggcatc cgcgggccgg ttatggtata ctgaagccat tgaatcaagg gcggggatcg
     3241 ggcgccccga acgcgaaagg gaagaaagga agccgaaaga tgcgagtaaa aaatgatctc
     3301 gaccgccgca agctggtcgc tgcggcgatg ggccaggtcc cctgcgacct gaccgtaaaa
     3361 aatgtgcgct ttgtcaacgt catcaccggc gaggtatacc ccgccgaggt ggacgtgttg
     3421 gacggcgtgg tggtgcgcgt gcgcgaaaag gaaaggcagg cggagctgga aagccgcacc
     3481 gtctacgacg gcggcgggcg ttacctggcc ccggggtgca tcgacatcca tatgcacgtc
     3541 gagagcacca tgatgacgcc ggaaaatttc ggcaaggccg ccgtgctctg cggcaccacc
     3601 agcgtctttg tggacccgca cgagatcgcc aatgtgttgg gcatcgacgg ggtgcggttc
     3661 atgatcgaca acgccaaggc ctcgccggtg cgccagttca acctggcgcc ctcctgcctg
     3721 ccctcggtgc ccggcgcgga gggcaacggc gccactttcg gcccgcagga gatcgcgcag
     3781 ctgctggata ccgagggcgt cttcggcgtg gcggagctga tggattttta caatgtgacc
     3841 cacggctctg aaaagatgcg cggcatcgcg gccgagggcc tggcgcggga tatgctcatc
     3901 cagggccacg cgcccaagct ggcgggcggc gccatcgccg cctatgcgct ggccggcccc
     3961 agcgacaacc acagcatccg cgacagctgg gaggtggtcg agaacctgca cgccgggctc
     4021 tatgtggacc tgcaggagaa ctcgctgggg gccagcacca tgccccagct ggtggagggc
     4081 ctgaaaggca tgcgctatac cgatcacgtc tgcctgtgca acgacgacgt gcacgccaag
     4141 gatctgctgg agaccggcca cgtgaaccgg ctgatcaagt cggtactgcg ccagggcgtg
     4201 cacccgctgg acgcgctgcg ctggtccacc tacaactcgg cccgcagcgc ccgcatcgac
     4261 gatttggggg ccctggcgcc gggctatgtg gcggacatgc agctgctgga cgagctggac
     4321 gggcgcgacc cctatgcggt cttttttgag ggcgcgctgg tggcggaggg cggcaagctg
     4381 gtggaggaaa agcccgcgca gcccatcccc gccgccgtgc gcggcggtac ggtgcgcgtt
     4441 gccggcctca ccggccccga ggctctggcc ctgcgcgccc ccgcgggcgc gggggaaacc
     4501 gtccgggtgg cggtgctgga ctatgacacc cccaccgccc gcagcgacct gttctatgag
     4561 gagctgccgg taaaggacgg gctggtggat ctctcgggcc gggacgacct ctgcttcatc
     4621 gcggtctgga acaggcacgg ctcgggcgag ggcaccgtgt cggtggcccg tcacctttac
     4681 ctgcgcgagg gcgcgatggc caccaccgtc agccacgact gccacaactt gaccctctgc
     4741 tataagcgcc cgcaggacgg ctatgcggcg gtgaaagccc ttgtcggctg cggcggcggt
     4801 ttttgcacgg tgaaagacgg ccaggtcacc ggcttgctgg agctgccggt tgccggcctg
     4861 atgagcgagc tgccctgccg cgagctggtg cctgccatcg cccggcaaga gcaggccacc
     4921 gacgcgatct ttgagcgccc gggccgcatg atgaagctct cgctgggcga tggcaggc
//
LOCUS       DBMDYA010000129         4972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4153_length_4972_cov_1.608910, whole genome shotgun sequence.
ACCESSION   DBMDYA010000129 DBMDYA010000000
VERSION     DBMDYA010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_4972_cov_1.608910"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2361)
                     /locus_tag="ACZPFK_04440"
     CDS             complement(<1..2361)
                     /locus_tag="ACZPFK_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1405591.1"
                     /translation="MLCVRCKKRPAVVFVQRMDGGETKTDGYCLTCAKELGIKPVDDL
                     MKQFGISDQDLENMEDRFSAFMQGGGAEAMEGLMESGSEDGDEPGEGEDDDFTPGGSA
                     TFPFNLMGRMGGEEARDADYHPVEGQDGTAGRTDKKDPRAKEKGKKRKFLDTYCENLT
                     GKAQEGKLDQIIGRDKEIYRAIQILCRRQKNNPCLIGEAGVGKTAIAEGIALRIAEGK
                     VPSRLEDKEIYLLDLTALVAGTQFRGQFESRVKGLISEVKAAGNVILFIDEIHNITGA
                     GDSEGAMNAANILKPALSRGEIQVIGATTFGEYRKYIEKDQALERRFQPVKVEEPSIA
                     DTTAVLMGIKQYYEKHHYVKISPEIVQMEVTLSERYITDRFLPDKAIDLMDEAAACCS
                     LRHPEITEYVNLEKHIAELDAERLAIEQKAGAEQADFERLANVKSELLRLGVERDAKK
                     AQVEQIEVEPEDVSKVIELWTGIPAVKIQQSDFFKINALEGNLKKKIIGQDEAVELVA
                     KAIKRSRAGIAGRQRPASFIFVGPTGVGKTELVKQLASELFDTVDPLIRLDMSEFMEK
                     FAVSRLIGSPPGYVGYDEAGQLTEKVRRRPYSVVLFDEIEKAHPDVMNILLQILDEGK
                     INDAQGRTVNFENTVICMTSNAGSSERIGETGFNKTAAQMSKDKAMKALSDFLRPEFI
                     ARVDEVISFNPLTEEDLQKIASLMLEEYRKPLQDKGVGLSYTPAALKLLCEKASGGKF
                     GARDLRRVIRKEVEDPIAERIVSGDAPREICIDAENGALHVTGEQIA"
     gene            complement(2531..3223)
                     /locus_tag="ACZPFK_04445"
     CDS             complement(2531..3223)
                     /locus_tag="ACZPFK_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1405592.1"
                     /translation="MTITSALILDLALLAVVIVTVIVAANRGFLATILKLCGTALALA
                     ASWLAASRLSAAVFENFFKNGMIDKTSQMISEGGQVSIQAVVDKLAAFLPDSLVQSLL
                     GGTEQLQGMLDSGAPGVASQVVDQVIAPLFLPIISVVVFFVTFAIASVLINFLVSALS
                     NLNRVPLMGGLNRTLGGVAGLVLGVVYVLLLLCAVWAVVVITSDGMPYFNTVTLQGSM
                     FYQFFFRYNPFV"
     gene            complement(3342..4742)
                     /locus_tag="ACZPFK_04450"
     CDS             complement(3342..4742)
                     /locus_tag="ACZPFK_04450"
                     /inference="COORDINATES: protein motif:HMM:NF039834.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1405593.1"
                     /translation="MGLGPKRQKKEKRLSSAEAFRRAAALERGELPPDGDAPEEEVRW
                     EEPGQTPPARQQELPAAKSKRRGKNKKEKKQPQSDMPRIEQIRRRKHERRLRRAALAL
                     AVLVAVFLYLGGVFSASVNLFSDLLDSARIALTPGGGWPVGMQVEGLLYAEPLSGGLA
                     LCGKSELVLYSPTARQLRSVQHGYAEPALAVGNARVCVYNRSGKELRVESRSRTLTTK
                     TTEYPILTVGMAPGGTMAVATRSERYLAELTVYDPNFEEVYYAYLAEYYPLSIAIASD
                     NKRMAVSCMKVENGVFGTQLQLYDMSKSAEEARVTVDVPDSVPLQLHYTSDGALVVIY
                     DGFAAVYDPATGQQTARYDYGGSSLLTADFHGRNTLLVFGEAGRASAGSCVLLGETMQ
                     VLATVSPGIDVRDAALDAEHFYLLGAKTAVGYDLTGAEVCREALPAEGMRMVCGKAPL
                     AVTAKEILQLTPLKNK"
     gene            complement(4733..>4972)
                     /locus_tag="ACZPFK_04455"
     CDS             complement(4733..>4972)
                     /locus_tag="ACZPFK_04455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921976.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1405594.1"
                     /translation="GDELRFMIEADGYLYNMVRILVGTLVEVGAGRRDPEVMPAVLAA
                     CDRAAAGQTAPAKGLALAKVFYDEFTVDGEGELWA"
BASE COUNT          830 a         1686 c         1472 g          984 t
ORIGIN      
        1 cgcaatctgc tcgccggtca cgtgcagggc gccgttttcg gcgtcgatgc aaatttcgcg
       61 cggcgcgtcg ccgctgacga tgcgctcggc gatcgggtcc tcgacttcct tgcggatgac
      121 ccggcgcagg tcgcgggcgc cgaacttgcc gccgctggcc ttttcacaca gcagcttcag
      181 cgccgccggg gtatagctca ggcccacgcc cttgtcctgc aacggcttgc ggtactcctc
      241 cagcatcagc gaggcgatct tctgcagatc ttcctcggtg agggggttga agctgatgac
      301 ctcgtccacg cgggcgatga actcgggccg caaaaagtcg ctcagcgctt tcatggcctt
      361 gtccttgctc atctgggccg ccgtcttgtt gaagccggtc tccccgattc gctcgctgct
      421 gccggcgttg ctggtcatgc agatcaccgt gttctcaaag ttcacggtgc ggccctgggc
      481 gtcgttgatt ttgccctcgt ccaggatctg cagcaagata ttcatcacat ccgggtgtgc
      541 cttttcgatc tcgtcgaaca gcaccacgct gtaggggcga cggcgcacct tttcggtgag
      601 ctgccccgcc tcgtcgtagc ccacatagcc cgggggcgag ccgatcaggc gcgagacggc
      661 gaatttttcc ataaattcgc tcatgtccag gcggatcagc gggtccacgg tgtcaaacag
      721 ctcgcttgcc agctgcttga ccagctcggt cttgcccacg ccggtgggac cgacaaagat
      781 aaagctggcc gggcgctgac ggccggcaat gccggcgcgg ctgcgcttga tggctttcgc
      841 caccagctcc accgcctcgt cctggccgat gatcttcttt ttcaggttgc cctccagggc
      901 gttgatcttg aagaagtcgc tctgctggat cttcaccgcc gggatacctg tccacagctc
      961 gatgaccttg gacacatcct ccggctcaac ctcgatctgc tccacctgtg cctttttggc
     1021 gtcccgctcg acgcccaggc gcagcagctc gcttttgacg ttggccagcc gctcaaagtc
     1081 cgcctgctcg gcgccggcct tttgctcgat agccagccgc tcggcgtcca gctcggcgat
     1141 gtgcttttcc agattcacat actcggtgat ctccgggtgg cgcaggctgc agcaggccgc
     1201 cgcctcatcc atcaggtcga tggccttgtc gggcagaaag cggtcggtga tatagcgctc
     1261 ggacagcgtc acctccatct gcacgatctc agggctgatc ttcacgtaat ggtgcttttc
     1321 ataatactgc ttgatgccca tcagcaccgc cgtggtgtcg gcgatggagg gctcctccac
     1381 ctttaccggc tgaaagcgcc gttccagcgc ctggtccttt tcgatatatt tgcggtactc
     1441 gccgaacgtg gtggcgccga tgacctggat ctctccgcgg gagagcgccg gcttgaggat
     1501 attggccgcg ttcatcgcgc cctccgagtc ccccgcgccg gtgatgttgt ggatctcgtc
     1561 gatgaacagg atcacgttgc ccgccgcttt gacctcgctg atcaggccct tgacccggct
     1621 ctcgaactga ccgcggaact gggtgcccgc caccagggcg gtcaggtcca gcagatagat
     1681 ctccttgtcc tccaggcggc tgggcacctt gccctcggcg atgcgcaggg cgatgccctc
     1741 ggcaatggcg gtcttgccca cgcccgcctc gccgatcagg caggggttgt tcttttgcct
     1801 gcggcagagg atctggatgg cgcggtaaat ttctttgtcg cgcccgatga tctggtccag
     1861 cttgccctcc tgcgccttgc cggtgaggtt ctcacagtag gtgtcgagga acttgcgctt
     1921 tttgcctttt tccttggcgc gggggtcttt tttgtcggtc ctgcccgccg tgccgtcctg
     1981 gccctccacg gggtggtaat cggcgtcccg ggcctcctcg ccgcccatgc ggcccatcag
     2041 gttaaagggg aacgtggcgc tgccgccggg ggtaaaatcg tcgtcctccc cctcgccggg
     2101 ctcgtccccg tcctccgacc cgctctccat caggccctcc atggcctctg caccgccgcc
     2161 ctgcataaag gcggaaaagc ggtcctccat attttcaaga tcctgatctg aaatgccgaa
     2221 ctgcttcatc aggtcgtcca ccggcttgat gcccagctcc ttggcgcagg tcaggcaata
     2281 accgtcggtt ttggtctcgc cgccgtccat gcgttggaca aagacgacag ccggccgctt
     2341 tttacagcgg acgcacagca tctgtgccat aagttatcac tccttctctt gcggcgcgtt
     2401 tcgcccgccg cccgcgtgcg catcaagcgc gcaaagcctc tctatccagg gatgagggcg
     2461 gcatttctta ctctgccccc cgccccgcgc agcacgcgcg gaaacaatta ccggccctgc
     2521 cgaagcggcc ctatacgaat gggttgtatc tgaaaaaaaa ctggtagaac atgctgccct
     2581 gcagggtcac cgtgttgaaa tacggcatgc cgtcgctggt gataaccaca acggcccaca
     2641 ccgcacagag cagcagcagc acatagacga cccccagcac caagccggcc accccgccga
     2701 gggtgcggtt cagcccgccc atcagcggca cgcggttgag gtttgagagg gccgagacga
     2761 gaaaattgat cagcacgctg gcaatggcaa aggtgacgaa aaacaccacc accgagatga
     2821 tgggcagaaa aagcggcgcg atcacctggt ccaccacctg cgaggccacg ccgggcgcgc
     2881 cggaatccag catgccctgc agctgttcgg tgccgcccag caagctctgc accaggctgt
     2941 cgggcaaaaa agccgccagc ttatccacca ccgcctggat cgagacctgt cccccctcgc
     3001 tgatcatctg cgaggtcttg tcgatcatgc cgttcttgaa aaagttttca aacaccgccg
     3061 cggacaggcg gcttgccgcc aaccaggaag cggccagcgc cagcgcagtg ccgcacagct
     3121 tcaggatcgt ggcgagaaag ccgcggtttg ccgccacgat caccgtgacg atgaccacgg
     3181 ccagcagtgc caggtcgaga atgagcgcgg atgtaatcgt cataaaccgg aacctcccgc
     3241 ccctctgggg ccacggctgc gccgccggcg gacagggctt ccccctccga cgcggcggcg
     3301 cggccgtttt tgtctatgat aaataggtat tatagcataa cttatttatt cttcaaaggt
     3361 gttaattgta aaatttcttt ggccgtgacc gccagcggcg ccttgccgca gaccatccgc
     3421 atcccctcgg cgggcaaggc ttcccggcag acctctgcgc cggtgaggtc gtatcccacc
     3481 gccgtcttgg cccccagcag ataaaagtgc tccgcatcca gcgcagcgtc ccgcacatca
     3541 atgccgggcg acaccgtggc cagcacctgc atggtctcgc ccagcagcac acagctgccc
     3601 gccgaagcgc ggcccgcctc gccaaagacc agcagcgtgt tgcggccgtg aaaatccgcc
     3661 gtcagcagag agctgccgcc gtaatcgtag cgggcggtct gctgcccggt agccgggtcg
     3721 taaacggcgg cgaagccgtc gtagatcacc accagcgccc cgtcggaggt gtagtgcagt
     3781 tgcagcggca cgctgtccgg cacatccacc gtcaccctgg cctcctctgc gctttttgac
     3841 atgtcgtaaa gctgcagctg ggtgccgaaa acgccgtttt ccactttcat acagctcacc
     3901 gccatgcgct tgttgtcgga ggcgatggcg atcgagagcg ggtagtactc agccagataa
     3961 gcgtagtaga cctcttcaaa attggggtca tagaccgtga gctcggccag gtagcgctct
     4021 gagcgggtgg cgaccgccat ggtgccgccc ggcgccatgc ccacggtgag gatggggtat
     4081 tcggtggtct tggtggtgag ggtgcggctg cgggattcca cccgcagctc tttgccgctg
     4141 cggttataca cgcagacccg ggcgttgccc accgccagcg caggctcggc atagccgtgc
     4201 tgcacactgc gcagctgccg cgccgtgggg gagtagagca ccagctccga cttgccgcag
     4261 agggcgaggc cgccgctgag gggctcggcg tagagcaggc cctccacctg catccccacc
     4321 ggccagccgc cgcccggcgt cagggcgatg cgcgccgagt ccagcagatc gctgaacagg
     4381 ttgaccgagg cggaaaacac gccgcccaga tacaaaaaga ccgccacgag cactgcaagc
     4441 gccagcgcgg cccggcgcag cctgcgctcg tgcttgcggc ggcggatctg ttcaatgcgc
     4501 ggcatatcgc tctggggctg ctttttttct tttttgttct tgccgcgccg cttgcttttc
     4561 gcggcgggca gctcctgctg tcgcgcgggg ggcgtctgcc ccggctcttc ccagcggacc
     4621 tcctcctcag gcgcgtcccc gtccggcggc agctcgccgc gctccagggc ggcggcgcgg
     4681 cgaaaggcct ctgcgctgga gagccgcttt tcctttttct gtcttttcgg ccctaagccc
     4741 atagctcccc ctcaccgtcc acagtgaatt catcgtaaaa gacctttgcc agggccagcc
     4801 cctttgccgg cgccgtctgc cccgccgcgg cccggtcgca ggctgcgaga acggcgggca
     4861 tcacctccgg atcccgccgc cccgcgccga cctccaccag ggtgcccacc agaatacgca
     4921 ccatattgta aaggtaaccg tccgcctcaa tcataaaacg aagctcgtcc cc
//
LOCUS       DBMDYA010000130         4901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4209_length_4901_cov_1.452500, whole genome shotgun sequence.
ACCESSION   DBMDYA010000130 DBMDYA010000000
VERSION     DBMDYA010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4209_length_4901_cov_1.452500"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="ACZPFK_04460"
     CDS             complement(<1..190)
                     /locus_tag="ACZPFK_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405595.1"
                     /translation="MKREDSTAFGTGVPLFLRLHKCVQAVSADKSAVFHKARPISFVI
                     AFFKILYQLARIRRTLKAI"
     gene            complement(433..1461)
                     /locus_tag="ACZPFK_04465"
     CDS             complement(433..1461)
                     /locus_tag="ACZPFK_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1405596.1"
                     /translation="MISFNNDYSEGACPEILAALASENLRQNPGYGLDGHCGAAAARI
                     RALGQCPDADVHFLVGGTPANLISLTAFLRPHQAAVAAESGHIAVHEAGSIEAAGHKV
                     CTVAAPDGKLTPALVRQVVEAHTDEHMVQPKLVFISDSTEVGTVYKKTELAALRQVCD
                     ELGLYLYLDGARLASALACAENDLSLADIAALTDAFYIGGTKNGMLMGEAVVVLNSEL
                     KAGYRHIMKQRGGMLAKGWLLGLQFETLLQDGLYFELARHANAMAQKLAAAFAAAGCG
                     FVAPSPTNQIFPILPLAAVKKLAARYGFELWGAPQGDTQATRFVCSWATREEDVDALI
                     ADLKAALA"
     gene            1751..2929
                     /locus_tag="ACZPFK_04470"
     CDS             1751..2929
                     /locus_tag="ACZPFK_04470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1405597.1"
                     /translation="MDYSSLGIKEEIRQAIDKMGFTELTEVQEKAIPLMLAGKDVVAK
                     APTGTGKTCAFGVPLIQGIDPALAKPQALVLCPTRELCLQITEELGSLTAFLPDIRIA
                     AVYGGQPIEKQIRRLKEGAQIVVATPGRMQDHMDHRTIDVRGIATVVLDEADEMLDMG
                     FYRDVMKIVDHLPARQHLHMFSATISREVMDISWKYQKNPEEITVEPVEESAPRIDQY
                     SLLTTGRNKLADMTEIMVKHGYTRAMVFCNTKYATEALCNQLIGLNFKAECLNGDMPQ
                     SERNKIMGRFRAGDVDVLVATDVAARGIDVSDVQVVFNYDLPQTNEYYLHRIGRTGRA
                     KKEGVSFLLYTAEDKKRVREMIRYTQSEVTEVRLQFGKLVEVQPNPWASGGIKVLYSN
                     "
     gene            2946..3407
                     /locus_tag="ACZPFK_04475"
     CDS             2946..3407
                     /locus_tag="ACZPFK_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1405598.1"
                     /translation="MSKNGERAYALFCEGYNCAQSTFAAFCEEMGLQEETALRLASGL
                     GGGVGRQREVCGAITGMTMAAGLLRGYHTPTSGPEKTQTYAMVQQLCAAFREKHGSMI
                     CRELLGLDRAEGSPKASPRTPQYYHDRRCGEFCRDAADLLAGFLAQEAEEK"
     assembly_gap    3944..3953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4354..>4901
                     /locus_tag="ACZPFK_04480"
     CDS             4354..>4901
                     /locus_tag="ACZPFK_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405599.1"
                     /translation="MSQIKIEHLHYTYENGFEPVFRDVSLTLDTDWRLGLVGRNGRGK
                     TTLLRLLAGELDSAGAIRAAVRFSYFPFPLPPGAQAQAALSVAREAVAPFAAMERRME
                     QLLARGDEAALTEYGELEARYAALDGYTVDALLTREAARLGVGEAALSRPFSTLSGGE
                     RVKLLLAALFLRKNNFLLIDEPT"
BASE COUNT          891 a         1522 c         1620 g          858 t
ORIGIN      
        1 ctattgcttt gagtgttctt cttatccgtg caagctgata aagaatcttg aaaaaagcta
       61 taacaaaaga tatcggacga gccttgtgga aaacagcaga tttgtctgcc gatacggctt
      121 gaacgcattt atgcaggcgc agaaagagag gtacacctgt cccaaatgcg gtggagtctt
      181 ctcgcttcac gacggggtct gcagcgaatg tcaagaaaaa atgggataga ggtttttcgt
      241 ctgcgcagcc tcctgcaaag ccatgcgccc cgcccggctg ctgccgggcg gggcgcatgg
      301 ttcatccggc gggcgctggg cccacggctt ctggatggtt ttttgtgtcg gcgggccggg
      361 cgcgctttta cgccccgcgg ggcggcaaaa aaaacgctgc ccgcggcgtg ttgccccgcc
      421 cggcaggcgg cctcaggcca gggcggcttt caggtccgcg atcagggcgt ccacgtcctc
      481 ctcccgcgtg gcccagctgc agacgaagcg cgtcgcctgc gtgtcgccct gcggggcgcc
      541 ccacagctca aagccatagc gcgcggccag cttttttacg gcggccaggg gcaggatggg
      601 aaagatctgg ttggtggggg agggggccac aaagccgcag cccgcggctg caaaggcggc
      661 cgccagcttt tgcgccatgg cgttggcgtg ccgcgccagc tcaaaataaa ggccgtcctg
      721 cagcagcgtc tcaaactgca gccccagcag ccagcccttg gccagcatgc cgccccgctg
      781 cttcatgatg tggcggtagc cggctttcag ctcgctgttc agcaccacca cggcctcgcc
      841 catcagcatg ccgtttttgg tgccgccgat gtaaaaggcg tccgtcaggg ccgcgatgtc
      901 cgccagcgac aggtcgtttt ctgcacaggc gagggcgctg gccagccgcg cgccgtccag
      961 ataaaggtag aggcccagct cgtcgcagac ctgacgcagg gcggccagct cggtcttttt
     1021 gtagaccgtg cccacctcgg tggaatcgga gataaagacc agcttcggct gcaccatgtg
     1081 ctcgtcggtg tgggcctcca ccacctgccg caccagcgcg ggggtcagct tgccgtccgg
     1141 ggcggccacg gtgcacacct tgtggcccgc ggcctcgatg ctgccggcct cgtgtacggc
     1201 gatgtgcccg ctctccgcgg cgacggcggc ctgatggggg cgcagaaaag cggtcaggct
     1261 gatgaggttg gccggggtgc ccccgacgag aaaatgcacg tccgcgtcgg gacactggcc
     1321 cagcgcgcgg atgcgcgcgg cggcggcccc gcagtgcccg tccaggccgt agcccgggtt
     1381 ctggcgcagg ttttcactcg ccagcgcggc caggatctcg gggcaggcgc cctcgctgta
     1441 gtcgttgttg aagctgatca tgcttcacat cccctctctt ttcttttaca tcggttggat
     1501 atgatattgc atctatgatt atagcatggc ggcggccaag tgagaagaaa aagctttgca
     1561 gccgtcccgc cgccgcgcgc ttttggccgg ggccccgcgg cgcgttgcaa tgccgcgccc
     1621 cggtgtgcta caataacaaa gacaacggcg tgccgcaaag ccgggcttgt atcaggaaag
     1681 ggcgccccat cccggccctg ccaaaggccg cgcggcggga agaacaaatc agacaagagg
     1741 tattcaggct atggactaca gttcccttgg catcaaagag gagatccgac aggccatcga
     1801 caagatgggc tttaccgagc tgaccgaggt gcaggagaaa gccatcccgc tcatgctggc
     1861 gggtaaggat gtggtggcaa aggcgcccac cggcaccggc aagacctgtg cgttcggcgt
     1921 gccgctcatc cagggcatcg acccggcgct ggcaaagccg caggcgctgg tgctctgccc
     1981 cacccgcgag ctctgcctgc agatcacgga ggagctgggc agcctgacgg cctttttgcc
     2041 cgacatccgc atcgcggcag tctatggcgg tcagcccatt gaaaagcaga tccgccggct
     2101 gaaagagggg gcgcagatcg tcgtggccac cccggggcgc atgcaggacc acatggacca
     2161 ccgcaccatc gacgtgcgcg gcatcgccac cgtggtgctg gacgaggccg acgagatgct
     2221 ggacatgggc ttttaccgcg atgtgatgaa gatcgtggac catctgcccg cccgccagca
     2281 cctgcacatg ttctcggcca ccatctcccg cgaggtgatg gacatcagct ggaagtatca
     2341 gaagaacccg gaggagatca cggtggagcc ggtggaggag agcgcccccc gcatcgacca
     2401 gtacagcctg ctcaccaccg gccgcaacaa gctggccgac atgaccgaga tcatggtcaa
     2461 gcacgggtac accagggcca tggtgttctg caacaccaaa tatgccaccg aggcgctctg
     2521 taaccagctc atcggcctca atttcaaggc ggaatgcctg aacggcgata tgccccagag
     2581 cgagcgcaac aagatcatgg gccgctttcg cgccggggat gtggacgtgc tggtggccac
     2641 cgacgtggcg gcccggggca tcgacgtgtc ggacgtgcag gtggtgttca actatgacct
     2701 gccccagacc aacgagtact atctgcaccg catcggccgc accgggcggg cgaaaaaaga
     2761 gggggtcagc tttctgctct ataccgccga ggacaaaaag cgggtgcggg agatgatccg
     2821 ctacacccag agcgaggtca ccgaggtgcg gctgcagttc ggcaagctgg tagaggtgca
     2881 gcccaacccc tgggccagcg gcggcatcaa ggtgctttac agcaattgaa agggaggcag
     2941 cggccatgag caaaaacggc gagagggcct atgccctgtt ctgcgaaggc tataactgcg
     3001 cccagtccac ctttgcggct ttttgcgaag agatggggct gcaagaggag accgcgctgc
     3061 ggctggccag cggccttggc ggcggcgtgg gccgtcagcg ggaggtctgc ggggcgatca
     3121 ccggcatgac catggccgcc gggctgctgc ggggctatca cacccccacc tccgggccgg
     3181 aaaagacgca gacctacgcc atggtgcagc agctctgcgc cgcctttcga gagaagcacg
     3241 gcagcatgat ctgccgcgag ctgctggggc tggacagggc ggagggcagc cccaaggcgt
     3301 cgccccgcac gccgcaatat taccacgacc gccgctgcgg cgagttctgc cgggacgcgg
     3361 ccgatctgct ggccggcttt ttggcgcagg aagcggaaga aaaatgacgc gcgccggggc
     3421 ggaagcaggc cctggggcgc gcggtttgtt ctccccccgc agggagccgg tgcggtctgc
     3481 ggcgccgggg gaaaaagcaa aaagcattgc cgccgaagga tagcactctc gtcgccggcg
     3541 cggcggctcc ctggagacgg aggccgcgcc gcggccgccg caggcgggct tgcggcgtcg
     3601 gcccggtaga cgcccgcggt gcgaagccgc gccgggcttt tgctttgcgg atgggccgcc
     3661 gcggcgctgt ttttgaaagc ggcggttgac aggcggccgc aggcatgcta tgatgatgcc
     3721 ataattttga gactggaagt ggaaagatgc gcaaataaca tccttttgac cgttcatgtg
     3781 cgggcgggct gcgttttggc gcggccggac ggcgcgtggc agaaaaggga ttgctattcc
     3841 gcatctttcc gtttttttta gaggatcgcc gggcctcggc ccgggtgcaa tcctttgaaa
     3901 gagagggcgc actggtgtgc gcccggcgga gccgcgggag gaannnnnnn nnntattgct
     3961 ttgcaataaa aacaatctat tttaacggcc ccgtttcttg cctctggcgc ggcaactgaa
     4021 acggatggcc gaaaaatagt ctacggctat ttttattgct ttgcaataaa aaaacttatc
     4081 ttgatggccc cgtttctcgc cgctggcgca gcaaccgaaa cggatggccg aaaaatagcc
     4141 tacggctatt tttattggcc gcgtttttcg ccgctctcca cccctccggt gtcccgcctc
     4201 cgccttgtcc gaaaaggcgg gtcctgccct gtcgcaggtt ttgccgcggc aggcaaaggc
     4261 ccgtatcccg gggcccgcgc cgcatgtgat ctgcccgggc cgcagcgccc cgccctgtgc
     4321 ggtggggcca cggccacaga cccaggaggt accatgtctc aaataaagat cgaacatctg
     4381 cactacacct atgaaaacgg ctttgagccc gtctttcgcg atgtgtccct gacgctcgac
     4441 accgactggc ggctaggcct ggtgggccgc aacgggcggg gcaagaccac cctgctgcgg
     4501 ctgctggccg gcgagctgga cagcgccggt gcgatccgcg ccgcggtgcg cttttcctat
     4561 ttcccgttcc ccttgccgcc gggcgcgcag gcgcaggccg cgctctcggt ggcaagggag
     4621 gccgtggccc cctttgccgc catggagcgg cgtatggagc agctgctggc ccgcggggat
     4681 gaggcggctt tgacggaata cggcgagctg gaggccaggt atgcagcgct ggacggctat
     4741 accgtcgacg cactgctcac ccgcgaggcg gcgcgcctcg gcgtggggga ggcggccctc
     4801 tcccgcccct tttccaccct ttcgggcggc gagcgggtaa agctgctgct ggcggccctc
     4861 tttttgcgca aaaacaattt tttgctcatc gacgagccca c
//
LOCUS       DBMDYA010000131         4878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4233_length_4878_cov_1.534436, whole genome shotgun sequence.
ACCESSION   DBMDYA010000131 DBMDYA010000000
VERSION     DBMDYA010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_4878_cov_1.534436"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="ACZPFK_04485"
     CDS             complement(<1..269)
                     /locus_tag="ACZPFK_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405600.1"
                     /translation="MDLKKQRRQYLFICLVYLFYYASYTSYFSFLTVYLKGWGYGTGD
                     IGLIMTCNSLINLISQPLLGQLSDTRFPIKRIVTVGMLVTIPGAFL"
     gene            complement(466..1503)
                     /locus_tag="ACZPFK_04490"
     CDS             complement(466..1503)
                     /locus_tag="ACZPFK_04490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405601.1"
                     /translation="MMKKIPVIIDCDPGHDDMMALVLAHGSGKLDIRAVTTVAGNNTI
                     QNTTQNALNVLHYIGADEIPVAMGYQNPMVRDHAQVMEALRVSRQKMRPQEQISAEAK
                     KITGASVHGVSGMDGFTFPAENPKKAEALHSVEMMAKVLRESDEKITLIATGPLTNLG
                     FLVRLYPELLQKVERISMMGGTSQFVLSRPFMEFNTFVDAEATKIVFESGVPITMFGY
                     DVTYRVLFNQQTVDRLRALGNHTGAMVADLLCYFRENHNKGLSRLNLGERAPIHDACA
                     VAGVIDPSLVTDSRRMHVDVNIGGEFFDGATVCDYTGVMEYLPKNVEVVYDMDTDRFL
                     DSLIEAAGRCL"
     gene            complement(1648..2580)
                     /locus_tag="ACZPFK_04495"
     CDS             complement(1648..2580)
                     /locus_tag="ACZPFK_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405602.1"
                     /translation="MNVWNIIFTTDFGFMIFRVTTPILFTALGAMISRRAGIMNISLE
                     GLMLVGALFGVIGSTYSQSVIVGVVTAVLASLLFSLLFAYFVLKLKSDLILTGIALNL
                     MASGGTVFLLTVLCGDKGVSTSMNSLVVPSVELPLIRHIPILGDILSGHNLLTYVALL
                     CVVLVYLLINKSPLGLQIRSVGENPDAAESVGISVIKTRFIALVFTGILSGLGGAYMS
                     MGYVSWFARDMAAGRGYMALSAQNLGNGTPIGTFLASFIFGIADALSSTLQVLSLPAE
                     FIQMIPYLTTVIGLILYAMSRKRRAAKARGKLRT"
     gene            complement(2581..3654)
                     /locus_tag="ACZPFK_04500"
     CDS             complement(2581..3654)
                     /locus_tag="ACZPFK_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405603.1"
                     /translation="MSVKQIEKVMDVAKTAAAVLLSFAATILLICVVSSTPLATVKDF
                     FLGPFASVRRIGNIVEMAIPILFTSCAICIMYSTGHINMGTSGCFYMGGLAASAVAFG
                     VRLIPGLHPAACMLFGGLVGALIAAIPAVLRIRWDINEVVSSLMVNYIVVYFGTWLLR
                     TFMLDPQAGFNASKAYEKSATLPILLPGTRIHVGLILSVAVVVFSYLFLKKTKWGYQI
                     RMMGYNSSFAVYSGMGVTGILLLSQMLGGFIAGLGGATEVLGLYTRFEWAAAPSTGFD
                     GIMVAAIAQRNPAMVPLAALFLAYIRVGADVISRTSDVPAEIVSIIQALIIMLVGAQM
                     FLSGLRHKQIVKASSRQLAKEGN"
     gene            complement(3644..>4878)
                     /locus_tag="ACZPFK_04505"
     CDS             complement(3644..>4878)
                     /locus_tag="ACZPFK_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405604.1"
                     /translation="KYKTLIDMEKAVRDTNEICEKYNLKVDPTARVRDLSVGLKQKVE
                     ILKALYRKAKVLILDEPTAVLTPQETAELFVELKKMKESGHTIIFISHKLNEVKELCD
                     RVTVLRLGRTIGTADLAGLSEQEISRMMVGRDVILEIDKEKARPRQPVLTVNGLSYHS
                     GDGRPVLNNVSFTVRAGEILGIAGVEGNGQRELSEIITGLLPFAQGEIGIEGKPIRGK
                     SVKQIRRMGVSHISEDRMTYGVAAKTSVRENVISDRFDQPEYCRAGFLRFGAIDQLTN
                     RLIQEFHIKCDDAGQPVRMLSGGNMQKVVAAREFSLDPHLAIVNQPTRGIDVGATEFV
                     RNKIVELRDKGTAVLLISADLNEILELSDSLIVMHGGEIVAYLPDSSQVTEDELGEYM
                     LGIRRQQPEEIRRVCHEC"
BASE COUNT          918 a         1496 c         1559 g          905 t
ORIGIN      
        1 agaaaagcgc cggggatggt gaccagcata cccaccgtga cgatgcgctt gatgggaaaa
       61 cgggtgtcgc tgagctgccc cagcagcggc tgggagatta ggttgatcag cgaattgcag
      121 gtcatgatca ggccgatgtc gcccgtgccg tagccccagc ctttcaggta taccgtaaga
      181 aaggaaaaat acgaggtata cgatgcgtaa taaaacagat agaccaggca gataaagaga
      241 tattgccgtc tctgcttttt cagatccatc aggctctcac atccattcca tctcaaattt
      301 caaaaagcaa aaacgccggc aaagtcctgt gcacaggcgc cgcgctttgg gccggcggcc
      361 gggagatgcg cggggcagca gggcaaaatt tgaccggcgt tttttcgtgg acgttctctt
      421 tccgtgtcct ggccccgggc tggaggcggt cgggccgtcc ccgccctaca ggcagcggcc
      481 cgcggcctcg atcaggctgt ccaggaaccg gtcggtgtcc atgtcgtaaa ccacctccac
      541 gtttttgggc agatattcca taacgccggt gtaatcgcag accgtggcgc cgtcaaagaa
      601 ttcgccgccg atattgacgt cgacgtgcat cctgcggctg tcggtgacca gcgaggggtc
      661 gatcacgccc gccaccgcgc aggcgtcgtg gatgggggcc cgctcgccca ggttcagcct
      721 gctcagcccc ttgttgtggt tttcacggaa atagcacagc aggtccgcca ccatggcgcc
      781 ggtgtggttg cccagcgccc gcaggcggtc gacggtctgc tggttgaaca gcacccggta
      841 ggtgacgtcg tagccgaaca tggtgatggg cacgccgctc tcaaagacga tcttggtcgc
      901 ctcggcgtcc acaaaggtgt tgaactccat aaacgggcgg gagagcacaa attggctggt
      961 gccgcccatc atcgagatac gctccacctt ttgcagcagc tcgggataca ggcgcacgag
     1021 gaagcccagg ttggtcagcg gcccggtggc gatcagcgtg attttttcgt cgctctcccg
     1081 cagcaccttt gccatcatct ccaccgagtg cagcgcctcc gcttttttcg ggttttccgc
     1141 cgggaatgtg aagccgtcca tgccgctgac gccgtggacg ctggcgccgg tgatcttttt
     1201 cgcctctgcg gagatctgct cctgggggcg cattttctgg cgcgagaccc gcagcgcctc
     1261 catcacctgg gcgtggtcgc gcaccatggg gttctgatag cccatggcca cggggatctc
     1321 atccgcgccg atatagtgca gcacgttgag cgcgttctgg gtggtgttct ggatggtatt
     1381 gttgcccgcc accgtggtca ccgcgcggat gtccagcttg ccgctgccgt gcgccagcac
     1441 cagcgccatc atgtcgtcgt ggccgggatc gcaatcgatg ataaccggga tctttttcat
     1501 cataagtatc aaccctttct gccgcgcggg aaaccgggta ggccccggcc ccgcagacgg
     1561 caaatcaaaa tttttaaaat tggcgaaacg gatgggaaca ggggcttttc accccacgcg
     1621 cgggcacggc ccgcgccccc ggccgcgtca cgtccgcagc ttgcccctcg ccttggccgc
     1681 gcggcgcttg cggctcatgg cgtagaggat caggccgatg acggtggtca gataggggat
     1741 catctggatg aactcggcgg gcaggctgag cacctgcagc gtgctggaca gcgcgtcggc
     1801 gatgccgaag atgaagctgg ccaaaaaggt gccgatgggg gtgccgttgc ccaggttctg
     1861 cgccgagagg gccatgtagc cgcgcccggc ggccatgtcc cgcgcaaacc aggacacata
     1921 ccccatggac atgtacgcgc cgcccaaccc cgagaggatg ccggtgaaga ccagggcgat
     1981 gaagcgggtc ttgatgacgc tgatgcccac cgattcggcc gcgtcggggt tttcacccac
     2041 cgagcggatc tgcagcccca gcgggctttt gttgatgagc agatagacca gcaccacaca
     2101 gagcagcgcc acataggtga gcaggttgtg gccggacagg atatcgccca aaatggggat
     2161 gtgtcggatg agcggcagct cgacgctggg caccaccagg ctgttcatcg aggtggatac
     2221 ccccttgtcg ccgcagagca cggtgagcag aaagacggtg ccgcccgagg ccatcaggtt
     2281 gagggcgatg ccggtgagga tcaggtcgga cttgagcttg agcacaaaat aggcgaagag
     2341 cagcgagaag agcaggctcg ccagcaccgc cgtcaccacg ccgacgatca cgctctggct
     2401 gtaggtgctg ccgatgaccc cgaagagcgc ccccaccagc atcaggccct caaggctgat
     2461 gttcatgatg cccgcgcggc ggctgatcat ggcccccagc gcggtgaaga ggatgggggt
     2521 ggtcacccgg aagatcataa aaccgaaatc cgtggtaaag atgatgttcc agacgttcat
     2581 tcagtttccc tcctttgcaa gctggcggga cgacgccttg acgatctgct tgtggcgcag
     2641 gccggagagg aacatctgcg cgcccacgag catgatgatc agcgcctgga tgatgctcac
     2701 gatctcagcg ggcacgtcgc tggtgcggga gatgacgtct gcgccgaccc ggatataggc
     2761 caggaacagg gccgccagcg gcaccatggc cgggttgcgc tgggcgatgg cggccaccat
     2821 gatgccgtca aagccggtgg agggcgccgc cgcccactca aagcgggtgt acaggcccaa
     2881 cacctcggtg gcgccgccga ggccggcgat aaagccgccc agcatctggg acagcagcaa
     2941 aatgccggtg acccccatgc cggagtagac cgcaaagctg ctgttgtagc ccatcatgcg
     3001 gatctgatag ccccatttgg tctttttgag gaacaggtag gagaagacca ccaccgccac
     3061 cgagaggatg aggcccacat ggatgcgggt gcccgggagg aggatgggca gggtggcgct
     3121 tttttcatat gccttgctgg cgttgaagcc cgcctggggg tccagcataa aggtgcgcag
     3181 cagccaggtg ccgaaataga ccacgatgta gttgaccatc agcgaggaga cgacctcgtt
     3241 gatgtcccag cggatgcgca gcaccgccgg gatggcggcg atgagcgcgc cgaccaggcc
     3301 gccgaacagc atgcaggccg cggggtgcag gccggggatc agccgcacgc cgaacgccac
     3361 cgccgaggcc gccaggccgc ccatgtaaaa gcagccggag gtgcccatgt tgatatggcc
     3421 ggtggaatac atgatgcaga tggcgcagct ggtgaacagg atggggatcg ccatctccac
     3481 gatgttgccg atgcggcgca cgctggcgaa cggcccgagg aaaaagtcct tgaccgtggc
     3541 cagcggcgtg ctgctgacca cgcagatcag caggatggtg gccgcgaacg agagcaggac
     3601 ggccgccgcg gtcttggcga catccatcac cttttcgatc tgtttaacac tcatggcaaa
     3661 ccctcctgat ctcttcgggc tgctgccgcc ggatgccgag catgtactcg cccagctcgt
     3721 cctccgtcac ctggctggaa tccggcagat aggccacgat ctcgcccccg tgcatcacga
     3781 tcagcgaatc gctgagctcg aggatctcgt tcaggtccgc ggagatcagc agcacggctg
     3841 tgcccttgtc ccgcagctcc acgatcttgt tgcgcacaaa ctcggtggcg cccacgtcga
     3901 tgccgcgggt gggctggttg acgatggcaa gatgcgggtc cagcgaaaac tcccgcgcgg
     3961 cgaccacctt ttgcatgttg ccgccggaca gcatgcgcac cggctggccc gcgtcgtcgc
     4021 acttgatgtg gaattcctgg atgagccggt tggtgagctg gtcgatggcg ccaaagcgca
     4081 gaaagcccgc ccggcagtat tccggctggt caaagcggtc ggagatgacg ttctcccgca
     4141 cgctggtctt ggccgccacg ccgtaggtca tgcggtcctc ggagatgtgg gagaccccca
     4201 tgcggcggat ctgcttgacc gacttgcccc ggatgggctt gccttcgatg ccgatctccc
     4261 cctgggcaaa gggcagcagc ccggtgatga tctcggacag ctcgcgctgg ccgttgccct
     4321 cgacgcccgc gatgcccaat atctcgccgg cccgcacggt aaagctcaca ttgttcagca
     4381 ccggccgccc gtcgccggag tggtagctga gcccgttcac ggtgagcacc ggctggcgcg
     4441 gccgggcctt ttctttgtcg atctccagga tgacgtcccg gcccaccatc atgcggctga
     4501 tctcctgctc gctgaggccg gccaggtcgg cggtgccgat ggtgcgcccc aggcgcagca
     4561 cggtcacccg atcgcagagc tccttgacct cgttcagctt gtgggagatg aagatgatgg
     4621 tgtgcccgga ctctttcatt tttttcagct caacaaagag ctccgccgtc tcctgcgggg
     4681 tgagcaccgc cgtgggctcg tccaaaatca gcaccttggc cttgcggtaa agcgctttca
     4741 ggatctccac tttctgcttg aggcccaccg acagatcccg cacgcgggcg gtggggtcca
     4801 ctttcaggtt gtatttttcg cagatctcgt tggtgtcccg caccgctttt tccatatcaa
     4861 tcagggtctt gtactttt
//
LOCUS       DBMDYA010000132         4862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4245_length_4862_cov_1.519639, whole genome shotgun sequence.
ACCESSION   DBMDYA010000132 DBMDYA010000000
VERSION     DBMDYA010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_4862_cov_1.519639"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="ACZPFK_04510"
     CDS             complement(<1..545)
                     /locus_tag="ACZPFK_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1405605.1"
                     /translation="MRVSGWSIAVNLALSAFKFFAGAAARSEAMLSDAVHSASDVLST
                     LVVMVGVKLAHRAPDTTHPYGHERFECVASLLLAAALALTGLGIGASALQRVLDSRTA
                     LPVPGMLALTAALVSIAVKEGMYCVTRAAAQKTGSSALMADAWHHRSDALSSVGSFAG
                     ILGARLGAPVLDPLAGLLICCL"
     gene            complement(689..2026)
                     /locus_tag="ACZPFK_04515"
     CDS             complement(689..2026)
                     /locus_tag="ACZPFK_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405606.1"
                     /translation="MAIHSMTQGPIPRQLLRFSVPMILGNLFQLTYNAVDTVIVGHCV
                     GKNAVAAVGTANPIMNVVIFFIGGICMGASVLMSEYYGAQDLRRLKREVSTTLLAGGL
                     FALVMSAVCALLARPVLLLIKTPAEIIDEAAGYLHIIFAGLLFTYLYNFYSNTLRSIG
                     DSRTPLLFLILSSALNIGLDLALVAGLGLGVRGAAIATVISQGVSGVLCILYIRLRIP
                     LLHFGRGELVLDPSMLARTLNYSWVTALQQTCIYIGKLAVQGAVNPLGVDSIAAFNAV
                     ARVDDFAITPMQSIGSALTTFVAQNHGAHNEGRIHKGLATGAAIGTAYWGVICAPIYF
                     GAGFVMHLFVPTGGEAVLALGAVYLHRMAFFYLFTAWTNSLQGYFRGRGLLTATLVSS
                     LLQIVIRVICSYWLAPATGIGGIALACGIGWTAMMLYELPLYLRDRGKRKLAA"
     gene            2218..3594
                     /locus_tag="ACZPFK_04520"
     CDS             2218..3594
                     /locus_tag="ACZPFK_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405607.1"
                     /translation="MENAKLNPMGTKPMFPLVMSMALPAMMSMLVQALYNIVDSYFVA
                     KVSEDALTAVSLVFPVQNLLIAVAIGTGVGLNSLISRRLGEGRLDDARAAAAHGLLLA
                     VCNWLIFAAIGLFAARPFISAFTANTAVVEMGVQYMSIVCAASLGMCMEGCIEKTLQA
                     TGNMIYPMISQMLGAVTNIILDPVFIFGLWGLPKMGVAGAAIATVLGQILAMFFLFFV
                     LAFREKVLKIDLRRFKFQGGIVRDIYAVGFPSIVMQSIGSLMTVGMNAILIRFTETAV
                     AFFGVYFKLQSFVFMPVFGLNQGVMPVIGYNYGARQRHRVTSAVKVGCSIAVCIMAAG
                     TVLFWAVPAQLLSIFNASETMLAIGVPALRIISTCFVAAALGILFSTAFQALGHGFNS
                     LIISVLRQLVILLPAAFLLARIGLEAVWLAFPIAELVALAASILLYLRLYKQVICPLG
                     QEQRPAAE"
     gene            complement(3817..4323)
                     /locus_tag="ACZPFK_04525"
     CDS             complement(3817..4323)
                     /locus_tag="ACZPFK_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1405608.1"
                     /translation="MRKNKVKWASLLRWLPAFLWMGAIFGFSAQNGEKSAGLSEAVEA
                     FLSALGGGAGTTGPFQFALRKGAHFCAYALLGMLCLLGARGAARLRQRRWRAPALALG
                     LSALYAATDELHQAFVPGRSAELRDVLLDTCGALSGILLALLFLRLSRRWGQGAEKPP
                     CAPERDKE"
     gene            4518..4838
                     /locus_tag="ACZPFK_04530"
     CDS             4518..4838
                     /locus_tag="ACZPFK_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405609.1"
                     /translation="MGEVRKEIAEKLLYYRKKKGITQRQLAESIGVKHNSISAWEKGT
                     NAIDIEVLYRICCVLGISISDIYGKYGSYGADDLSFTERQIVEAYRGQPEMQTAVQRV
                     LDIH"
BASE COUNT          966 a         1550 c         1428 g          918 t
ORIGIN      
        1 aggcagcaga tgagcaggcc ggccaaaggg tccagcaccg gcgcgcccag ccgggcgccc
       61 agaatgcccg caaagctgcc caccgaagag agcgcgtcgg accggtggtg ccaggcgtcc
      121 gccatcaagg cgctggagcc ggttttttgg gccgcagcgc gggtgacgca gtacatcccc
      181 tcttttaccg cgattgaaac cagcgccgcc gtcagcgcca gcatccccgg caccggcaaa
      241 gccgtccggc tgtccagtac ccgttgcagg gccgaggccc cgatccccag cccggtgagg
      301 gccaatgcgg cggccaacaa cagcgaagcc acgcattcaa accgctcatg gccgtaggga
      361 tgggttgtat ccggggccct gtgggccagc tttaccccca ccatcaccac cagggtgctg
      421 agaacatccg acgccgaatg caccgcatcg gagagcatgg cctctgaacg ggccgccgcc
      481 ccggcaaaaa acttgaacgc cgaaagagcc aggttgaccg cgatgctcca gcccgacacc
      541 cgcattgcca agcgctctga acccccctgc cccatgccgc tccacccccg cattgtctcc
      601 agtctatgcg gcggcagcga aaaaggtgcg gtcgtcaagg cctcacaatt ttatgttaaa
      661 agcaagttta aatttaaaca agggggattt atgccgcgag ttttcttttg ccgcggtcgc
      721 gcagatagag cggcagctcg tagagcatca ttgccgtcca gccgatgccg caggccaggg
      781 caatgccgcc gatccctgtc gcgggggcca gccaataaga gcagatcacg cgaatgacga
      841 tctgcaggag gctgctgacc agcgtggccg tcaacaggcc gcggccgcgg aaatagccct
      901 gcagcgaatt ggtccaggcg gtgaagagat aaaaaaacgc catgcggtgc aaatagacgg
      961 cccccagggc cagcacggct tcgccgccgg tgggcacgaa gagatgcatg acaaagcccg
     1021 ccccaaaata gatgggcgcg cagatgacgc cccagtaagc cgtcccgatc gcggcgccgg
     1081 tggcaaggcc cttgtggatg cgcccctcat tgtgggcgcc gtggttctgc gccacaaagg
     1141 tggtcaaggc gctgccgatg ctctgcatcg gggtgatggc gaaatcgtcc acccgggcca
     1201 ctgcattgaa agcggcgatg ctgtccaccc ccagcgggtt gacggccccc tgcacagcca
     1261 gcttgccgat gtagatacag gtctgctgca gcgccgtgac ccagctgtaa ttcagggtgc
     1321 gggccagcat ggaggggtcg agcaccagct cgccgcggcc gaagtgcagc agcgggatgc
     1381 gcaggcggat atacaggatg cagagcaccc cggagacccc ctgtgagatc acggtggcga
     1441 tggccgcgcc gcgcaccccc agccccaggc cggccaccag cgccaggtcg aggccgatgt
     1501 tcagcgcgct ggacaagatc aggaacagca gcggcgtgcg gctgtcccca atgctgcgca
     1561 gtgtattgga ataaaaattg tatagatagg tgaacagcaa gccggcaaag atgatgtgca
     1621 gataaccggc cgcctcgtcg atgatctccg ccggtgtctt tataagcagc agcaccgggc
     1681 gcgccagcag cgcgcacacc gccgacatca ccagcgcaaa cagcccgcca gccagcaggg
     1741 tggtgctcac ctcccgcttg aggcggcgca ggtcctgcgc gccgtaatac tcgctcatca
     1801 gcaccgaggc ccccatgcag atgccgccga taaaaaagat caccacgttc atgatagggt
     1861 tcgccgtgcc cacggcggcg acggcgtttt tgcccacgca gtggcccacg atcaccgtgt
     1921 ccaccgcatt ataggtgagc tggaacagat tgcccaggat catcggcacc gaaaagcgca
     1981 gcagctgccg cggaatcggc ccctgtgtca tgctgtggat cgccatccct cttcctcccg
     2041 caggccgggg aattgttccc ctgtattcaa ggcgctcttt attatagtgc ggttccgccg
     2101 ctttgtaaat ggttcggtga caaaatcata ctcttttctt tactttctat tgcatccgat
     2161 gcaaaaaccg tgtaaaatag gaaagttgat atcatcttga cgaatggagt gaattttttg
     2221 gagaatgcga agctgaaccc catgggtacc aagccgatgt tcccgctggt catgagcatg
     2281 gcgctgcccg ccatgatgtc catgctggtg caggcgctgt acaacatcgt cgacagctat
     2341 tttgtcgcca aggtcagcga ggacgcgctc actgccgtct cgctggtgtt cccggtgcaa
     2401 aacctgctga tcgcggtcgc cattggtacc ggcgtgggcc tcaactccct gatcagccgc
     2461 cgcctgggcg aggggcgact ggacgacgcg cgcgccgccg cggcccacgg cctgctgctg
     2521 gctgtctgca actggcttat cttcgccgcg atcggcctgt tcgccgcgcg gccgttcatc
     2581 tcggctttca ccgccaacac cgccgtggtg gagatggggg tgcagtacat gtccatcgtc
     2641 tgcgccgcct ctctgggcat gtgcatggag ggctgtattg aaaagaccct gcaggccacc
     2701 ggcaacatga tctatcccat gatctcccag atgctgggcg cggtgaccaa catcatcctg
     2761 gacccggtct tcatcttcgg tctttggggc ctgcccaaaa tgggggtcgc gggcgcggcc
     2821 atcgccacag tgctggggca gatcctggcg atgttcttcc tgttcttcgt gctggcgttc
     2881 cgcgagaaag tgctcaagat cgatctgcgc cgcttcaaat tccagggcgg catcgtgcgg
     2941 gatatctacg cggtgggctt cccctccatc gtcatgcagt cgatcggctc gctgatgacc
     3001 gtgggcatga acgccatcct gatccgcttt accgagacgg cggtcgcgtt tttcggcgtt
     3061 tacttcaagc tgcagtcctt tgtattcatg ccggtgttcg gcctcaacca gggcgtgatg
     3121 ccggtcatcg gctacaacta cggcgcccgg cagcgccacc gggtgaccag cgccgtgaaa
     3181 gtgggctgtt ccatcgccgt ctgcatcatg gcggcgggca ccgtgctctt ctgggccgtc
     3241 ccggcgcagc tgctctcgat cttcaacgcc tctgagacca tgctcgccat cggcgtgccg
     3301 gcgctgcgga tcatcagcac ctgctttgtc gccgccgccc tgggcatcct gttctccacc
     3361 gctttccaag ctttggggca tggcttcaac agcctgatca tctcggtgct gcgccagctg
     3421 gtcatcctgc tgcccgccgc gttcctgctc gcgcgcatcg gcctggaggc cgtctggctc
     3481 gcattcccca tcgccgagct cgtcgccctg gcggccagca tcctgctcta cctgcggctc
     3541 tataagcagg tcatctgccc gctggggcaa gagcagcggc ccgccgcgga gtgatccgtt
     3601 catcagaaag cgctcgtcct ttctcttcta cataaaaaaa cggtctccgc tatgcggagg
     3661 ccgttttttt gccccctccc caagacctct ccggggggag aagcacggcg gctgaggcgg
     3721 actgcatttt tggccggtgt ggaaatccac tatttctgcc gctttttcat cgtcacaaaa
     3781 acggcagaaa cggaggggag caagagaaac ggtcccctac tccttgtctc gctccggggc
     3841 gcagggaggt ttttctgcgc cctgtcccca ccggcggctc agccgcaaaa agagcagggc
     3901 caacaggatg ccgctcagcg cgccgcaggt gtccagcagc acatcccgca gctcagcgct
     3961 tcggcccggc acaaaggcct gatgcagctc gtctgtcgcc gcatagagcg ccgagagccc
     4021 caacgccagc gccggggcgc gccaccggcg ctggcgcagg cgcgccgcgc cccgggcccc
     4081 caacagacag agcatcccga gcagggcata agcacaaaaa tgcgcgccct tgcgcagcgc
     4141 gaattgaaag gggcccgtcg tgcccgcccc tccgccgaga gcggagagaa aagcttccac
     4201 cgcttcgctc aggcccgccg atttttcgcc gttttgtgct gaaaatccaa agatcgcccc
     4261 catccacaaa aaggccggca gccatcgcag cagagaggcc catttcactt tattctttct
     4321 caccgctttt ctcctttttt ccctatttta caaagaaata tgacaacatc tctaaagatt
     4381 aacggaatct taacggccat gtcgttgcag gcggtatttc catatgatat catgaattca
     4441 gagttcaggg aatgccgtcg ccttgcggcg gcttttccca ctttgcgttt tcccggtctg
     4501 aaaagagggg gattgacatg ggagaagtca gaaaagagat tgccgaaaaa ttgctgtact
     4561 atcgcaaaaa gaaagggatc acccagcggc agcttgcaga gagtatcggg gtcaagcaca
     4621 actctatctc cgcgtgggaa aagggcacca acgccatcga catcgaggtg ctgtaccgca
     4681 tctgctgtgt gctgggcatc tccatttcgg atatttatgg caaatacggc tcttacggcg
     4741 ccgacgacct gagctttacc gagcgccaga tcgtggaagc ctaccgcgga cagccggaga
     4801 tgcagacggc cgtgcagcgg gtcttggata tccattaaag ttcaaaaaag cagaaatgct
     4861 cc
//
LOCUS       DBMDYA010000133         4860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4250_length_4860_cov_1.612524, whole genome shotgun sequence.
ACCESSION   DBMDYA010000133 DBMDYA010000000
VERSION     DBMDYA010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_4860_cov_1.612524"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="mutY"
                     /locus_tag="ACZPFK_04535"
     CDS             <1..986
                     /gene="mutY"
                     /locus_tag="ACZPFK_04535"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1405610.1"
                     /translation="YYVWLSEIMLQQTRVEAATPYFERFIAELPSIADLAAAPEEQLL
                     KLWEGLGYYNRVRNLQKAAQMVVDRYGGQLPADFDALLSLPGIGRYSAGAIASIAFGL
                     PVPAVDGNVLRVLSRVLESPEDIGREETKRAMGDALKQVLPPARPGDFNQALMELGAM
                     VCLPNGAPRCDACPLQEQCLARRRGTIDRYPHKAPKKARRAEERTIFLLVQHGLVAVQ
                     KRPAAGLLRGLWELPGAEGFLDEAAARAHLTRLGLSPAGPLRDAGGAKHIFTHVEWRM
                     RGWAAPVRGEAPGLVFAAPERIFDELCLPSAFRAYTGQIETFVKEFTDADS"
     gene            973..1407
                     /locus_tag="ACZPFK_04540"
     CDS             973..1407
                     /locus_tag="ACZPFK_04540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007853.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR1405611.1"
                     /translation="MQIREYLPEDREKTAALWLTSTIKAHPFIPEEYWLRAYREVYHR
                     WLPETKTLLAEVDGEIEGFLSFTAPGEIGALFVGPSCQGKGHGTALLDAAKERFVRLQ
                     LSVYAQNSRAVAFYEKNGFVPQAERVEESTGERELVMVWHRP"
     gene            complement(1549..2208)
                     /locus_tag="ACZPFK_04545"
     CDS             complement(1549..2208)
                     /locus_tag="ACZPFK_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1405612.1"
                     /translation="MREIAKELEQASLFAGLGEREIEKLLGCLGARQALYRGGDLIVS
                     EGDEVETFGILLSGHARSLKWDACGREIILTLLQKGSEIGVMLAAGRDHKSPVTVQAQ
                     DEVRVLHVPFEHVFARCEKACPCHDRLLRNYVRIVAEKGLVLHERINCLLRPTVREKI
                     LVYLRRASGGRERERFTIPLDRNGMAQYLNVERSALSRELARMRRDGLIEYHRSTFCL
                     L"
     gene            2298..2843
                     /locus_tag="ACZPFK_04550"
     CDS             2298..2843
                     /locus_tag="ACZPFK_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405613.1"
                     /translation="MNKKTLWITRTAALTALLVVLQASTAALGSTLVTGTVVNLLLII
                     SVMTCGAASGLSVAVLSPVLAKLVGIGPLWGLIPFIAAGNLALATVWLAVGSRRWGPR
                     YAPQITALALAAAAKSLVLYLGVVKLALPLFSGLTAAQAAAIAHMFSAPQLVTAVLGG
                     LLALPLLAPLKKAMERGARQN"
     gene            2951..3328
                     /locus_tag="ACZPFK_04555"
     CDS             2951..3328
                     /locus_tag="ACZPFK_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR1405614.1"
                     /translation="MFAGVLPQFISVLLFSGLLLALLPPQAIGRLLGREAGLGGMLFS
                     ALVGAVTLVPVMVVFPLVGRLLEDGAGIAQMAVFISTLTTVGVVTLPMEIRYWGKKAA
                     LLRNLLAFLAAFLIACGLEVLLK"
     gene            3325..3858
                     /locus_tag="ACZPFK_04560"
     CDS             3325..3858
                     /locus_tag="ACZPFK_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR1405615.1"
                     /translation="MKTAVKKHGPLLTALLLHGAALLLAPRAEKEALSFCGQNLLNFF
                     LVLPPVFLCVGLIDAWVSRDRMMSALGENSGVKGAVFALAAGMAAAVPFYALLPVAGL
                     LLKKGSRILNVLIFLGASASLRIPLLLFEISSLGLRFALARLGLNLCAVFATAFLTEK
                     LLSEEDKRAVYEKTRDL"
     gene            4199..>4860
                     /locus_tag="ACZPFK_04565"
     CDS             4199..>4860
                     /locus_tag="ACZPFK_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014325.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1405616.1"
                     /translation="MNIVIVGIGKVGSTVAEYLSREGHEVVVVDRNPDVVDHMASNYD
                     VLGICGNGASIEIQKEAGVPKADLIVALTSSDEFNILCCILAKKSGARHAIARVRNPD
                     YFKQLGFMRKELGLSMAVNPELEAANAISRMLRFPSATKLELFAKGRLEMAEATLFPG
                     TLLEGVALHELQQKFGAQVLICAVQRGDEVFIPSGEFVLHAGDHINITASHADISTFF
                     KSL"
BASE COUNT          828 a         1568 c         1482 g          982 t
ORIGIN      
        1 cttattatgt ctggctgtcc gagatcatgc tgcagcaaac ccgggtggag gccgcgaccc
       61 cctattttga gcgctttatc gccgaactgc cctccatcgc cgatctcgcc gccgcccctg
      121 aagaacagct tttaaagctg tgggaggggc tgggatacta caaccgggtg cgcaacctgc
      181 aaaaggccgc ccaaatggtg gtggaccgct acggcgggca gctgcccgcc gacttcgacg
      241 ccctgctgtc cctgcccggc atcggccggt attccgccgg ggcaatcgcc agcatcgcgt
      301 tcggcctgcc ggtgccggcg gtggacggca acgtgctgcg ggtgctcagc cgggtgctgg
      361 aaagcccgga ggacatcggc agggaggaga ccaaacgggc catgggcgac gccctgaagc
      421 aggtcctgcc ccccgcccgc cccggcgact tcaaccaggc gctcatggag ctgggggcca
      481 tggtctgcct gcccaacggc gcgccgcgct gcgacgcctg ccccctgcag gagcaatgcc
      541 tggcgcggcg ccgcggcacc atcgaccgct atccgcacaa ggcccccaaa aaggcccggc
      601 gcgctgaaga gcgcaccatc tttctgctgg tacagcacgg cctggtcgcg gtgcaaaaac
      661 ggccggccgc cggcctgctg cgcggcctgt gggagctgcc gggcgcagag ggctttctcg
      721 acgaggcggc agcgcgggcc catctcaccc gattgggcct ctcccctgcc ggccccttgc
      781 gggacgcggg cggggcgaaa cacatcttca cccatgtgga gtggaggatg cgcggctggg
      841 ccgccccggt gcggggcgag gcgcccgggc tggttttcgc cgcgccggag cgcattttcg
      901 acgagctctg cctgcccagc gcttttcgcg cctacaccgg gcagatcgag acctttgtaa
      961 aggagtttac cgatgcagat tcgtgaatac ctgcccgagg atcgggaaaa gaccgccgcc
     1021 ctctggctga ccagcaccat caaggcgcat ccctttatcc cggaggaata ctggctgcgc
     1081 gcctaccggg aggtatacca ccgctggctg cctgaaacca aaaccctgct cgccgaggtg
     1141 gacggggaga tcgagggctt tctcagcttt accgcccccg gcgagatcgg cgcgctcttt
     1201 gtcggcccca gctgccaggg caaagggcat ggcaccgccc tgctggacgc ggcaaaggag
     1261 cgctttgtcc gtctgcagct gagcgtctat gcacaaaatt cccgggcggt cgctttctat
     1321 gaaaaaaatg gttttgtccc acaggcggag cgggtcgagg aatccaccgg cgagcgcgag
     1381 cttgtgatgg tctggcatcg gccctgacgc cgcgctttct gcgcttgttt tcacgaagcg
     1441 ccttgccctg tcccgggccc gggaccttga aaaagcttta aaatacaaaa agccgcgggg
     1501 gatcgcctcc cgcggctttt ttgtgccgca aagcgctttc cctgtctctc agagcaggca
     1561 aaaagtgctg cggtggtatt cgatgagccc gtcgcgccgc atgcgggcca gttcccttga
     1621 aagcgcgctg cgctccacat tgagatactg cgccatcccg ttcctgtcca gcggtatggt
     1681 gaagcgctcc ctttcccgcc cgccggaggc cctgcgcaga tagaccaata ttttttcccg
     1741 caccgtgggc cgcagcaggc agttgatccg ctcatgcagc accaggcctt tttcggccac
     1801 gatgcggaca tagttgcgca gcaaccggtc gtgacagggg caggcttttt cgcagcgggc
     1861 aaaaacatgc tcgaacggaa cgtgcaaaac ccgcacctcg tcctgcgcct gcacggtgac
     1921 agggcttttg tgatcccggc ccgcggccag catcacgccg atctcgctcc ccttttgcag
     1981 cagcgtgagg atgatctcgc ggccgcaagc atcccatttg agagagcgcg catgtccgga
     2041 caggaggatg ccgaacgtct ccacctcatc tccctccgag acgatgagat cgccgccgcg
     2101 gtaaagggcc tgcctggccc caaggcaccc gagcaatttt tcgatttccc tttccccgag
     2161 tcccgcaaac agacttgcct gctccagctc cttggctatt tcccgcatga ggccctcctt
     2221 tgttgcctgt gcaacaagaa atctgagcca atcatattat aatgaacggg aaaaatcaag
     2281 tgcagaggag tcatcacatg aataaaaaaa cgctttggat cacccgcacc gccgccttaa
     2341 ccgctttgtt ggtggtgctg caggcgagta ccgcggccct gggcagcact ctcgtcacgg
     2401 gtaccgtcgt gaacctgctg ctcatcatct cggtcatgac ctgtggggcg gcgtccggcc
     2461 tgtcggtcgc cgttctctcg ccggtgctgg caaagctggt cggcataggg ccgctctggg
     2521 gcctgatccc ttttatcgcc gccggcaact tggcgcttgc tacggtctgg ctggcggtgg
     2581 gcagcaggcg ctgggggccg cgctacgcgc cccaaataac ggccctcgcc ctcgccgcgg
     2641 ccgccaagtc ccttgtgctg tacctggggg tcgtaaagct cgccctgccg cttttttccg
     2701 gcttgacggc ggcgcaggcg gcggccatcg cccatatgtt ttccgccccg cagctcgtca
     2761 cggccgttct cggcggcttg ctggcgctgc cgcttctggc cccgctcaaa aaggcgatgg
     2821 aaagaggtgc gcggcaaaat tgaacaccgt ttttatctgc gcgctttacg cgacggcatt
     2881 gctttttctg cttgcctctc tcctcaggga caggcgcaaa acccggctgg ccctgaaaaa
     2941 ggccgttcat atgttcgcgg gtgtgctgcc gcaattcatc tcggtcctgc ttttttccgg
     3001 cctgctgctg gcacttcttc cgccccaggc gatcggccgt ctgctgggcc gggaagcggg
     3061 ccttggtggg atgctcttct ccgcccttgt gggcgccgtc acgctggtgc cggtcatggt
     3121 cgtcttcccc ctggtgggcc ggcttctcga ggacggcgcg gggatcgcgc agatggcggt
     3181 cttcatctcc accctcacca cggtgggagt cgtcaccctg cccatggaaa tccggtattg
     3241 gggcaaaaaa gcggcgctgc tgcgcaatct gctggcgttt ctggccgcct ttctcatcgc
     3301 ctgtgggctg gaggtgctgc tgaaatgaaa accgctgtaa aaaaacatgg tccccttttg
     3361 acggcgctgc tcctgcacgg ggccgcgctg ctgctggcgc cgcgggccga aaaggaggcc
     3421 ctgtcttttt gcgggcaaaa cctcctcaac ttttttctcg tgcttccccc tgtctttctc
     3481 tgcgtcggcc tgatagacgc ctgggtcagc cgggacagga tgatgagcgc tttgggcgaa
     3541 aactccggcg tcaaaggggc ggtgttcgcc ctggcggcag gcatggccgc cgccgtccct
     3601 ttctatgcgc tgctgccggt ggccgggctg cttttgaaaa aagggagccg catcctgaac
     3661 gtgctgatct ttctcggcgc cagcgccagc ctgcgcatcc ccctgctgct cttcgagatt
     3721 tcgtcgctgg gcctgcgctt tgcactggcc cggctggggc tcaacctctg cgccgtcttc
     3781 gccaccgcct ttctgacgga aaagctgctg tcggaggagg ataaaagggc cgtttacgaa
     3841 aaaacgcggg acctgtgacg gcaagcctgc aaacctgtcc ctgccggctt ttaccgcggc
     3901 tcgtcggccg ggagcgccca agaccaaccc gcgtgaagcc cgcaaagccc agccgccaaa
     3961 agggttcccc gcgcctgccg ctccttttct tttttgtgct ttctcgccgt gtttttcctt
     4021 gctgcggagg gcgcccttat ccttgggcgc cctctctttt tcattttact gttgcattgc
     4081 ccatgacaaa gttgtatcat gaaatcgcat taaaattgta ttaagaaaat tgtcgatttt
     4141 attaatacgg cgttaatatg acccggacaa acgactgggc ggcaactgga ggaacaatat
     4201 gaatatcgtg atcgtcggca tcggcaaggt gggcagcacc gtggccgagt acctctcccg
     4261 cgaggggcac gaggtggtcg tggtggaccg caaccccgat gtggtggacc acatggcgag
     4321 caattacgat gtgctgggca tctgcggcaa cggtgcttcc atcgagattc aaaaagaggc
     4381 gggggtgccc aaggccgatc tcattgtggc gctgacctcc tctgacgagt tcaacatcct
     4441 ctgctgtatc ctggcaaaaa agagcggcgc gcgccacgcc atcgcccggg tgcgcaaccc
     4501 cgactatttc aaacagctgg gctttatgcg caaggaattg ggcctttcga tggccgtcaa
     4561 cccggagctg gaggccgcca acgccatcag ccgcatgctg cgcttcccca gtgcgaccaa
     4621 gctggagctg ttcgccaagg ggcgcctgga gatggccgag gccacgcttt ttcccggcac
     4681 cctgctggag ggggtggcgc tgcacgagct gcagcagaaa ttcggcgccc aggtgctgat
     4741 ctgcgcggtg cagcgagggg atgaggtctt catcccctcg ggcgaatttg tgctgcacgc
     4801 gggcgaccac atcaacatca cggcctccca cgcggatatc agcacctttt tcaagagcct
//
LOCUS       DBMDYA010000134         4851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4258_length_4851_cov_1.601684, whole genome shotgun sequence.
ACCESSION   DBMDYA010000134 DBMDYA010000000
VERSION     DBMDYA010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_4851_cov_1.601684"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ACZPFK_04570"
     CDS             complement(<1..268)
                     /locus_tag="ACZPFK_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405617.1"
                     /translation="MTLTSAGYALFLPLVALAYYLAPGRLQNAVLLLSSCLFYGFGLG
                     GHPLSAAVLALHVLFTYLMARRIGAAKGARKKRLMALAVVSVVAV"
     gene            588..1571
                     /locus_tag="ACZPFK_04575"
     CDS             588..1571
                     /locus_tag="ACZPFK_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HIR1405618.1"
                     /translation="MSHTVMVTGADGFIGSHLTEELVRSGERVKAFCYYNSFNSCGWI
                     DTLPPEIRDEIEIFMGDIRDPNGVRTAMAGQERVYHLAALIAIPFSYHSPDSYVDTNI
                     KGTLNVLQAARDLGTQRLMITSTSEVYGTARYVPIDEHHPYQGQSPYSATKIGADRLA
                     ESFYRSFDLPVSIVRPFNAYGPRQSARAVIPTIIIQLLAGRTEIRLGNLTATRDFNYV
                     KDTAAGFMAIADCAEAVGQEINIATGREISIGELADELIRQINPAAKVVCEEQRLRPA
                     KSEVERLLGDATKLRQMTGWAPRYTFEEGLAETIAFLRGHLDSYKIDAYNL"
     gene            1614..3212
                     /locus_tag="ACZPFK_04580"
     CDS             1614..3212
                     /locus_tag="ACZPFK_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1405619.1"
                     /translation="MDKALQKLKSLWGRAWVRWALGYLFVLLCGVGVVYYWRVAYGLD
                     SLSPWVATAVVCGGFSAVYAVVFLIAHFVRQNLALKASLLVLVAGLCFVFANPPLQAP
                     DENMHFLRAYSIGSGNFLFEQNEDYPDDVDLLIQDFPGLYNHELIATGQATMADGFAR
                     YRAHLQQGATAPNAATLIQQLVPYLAQAPAIAAARLFGGDALACMYAARAGNLLLYAA
                     LCYVAFRAAKRFLPILITLAISPIALFTAGSCSADGLLLGLTWVFIGFCFSDRLSRGQ
                     VAALAVSFGLMVFAKYTPVAMLPLVALIPQPEGLRAGRGKTARSRWLVAAACLAGAAL
                     VYALLTAYTGFASNYGALPYADTGVRPGEQLRFILSNPPRYLAVFLYSLYRDKANLSM
                     LGNFGWMDMNVPFVSVFSPLVLLFSAGMSALEGAREKPRTGWACALTAALLYAFTYAG
                     MYLTSTPVSLPEINGVQSRYLLGAFFALLVLAAMLLGRTMALDKLRPGQPQKTPPAWR
                     MLHLAFCFSLVSALLLFQSYYIGP"
     gene            3292..>4851
                     /locus_tag="ACZPFK_04585"
     CDS             3292..>4851
                     /locus_tag="ACZPFK_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405620.1"
                     /translation="MTAFIEMFITFAALAALCLFVGARTSLPSGAAPLAVLCATMLWY
                     SLMGCFDLLFPAGLLWFAAALAAAAALALRRKEIRWKELFGPGMVFFLIAGLVVIGLF
                     ALRQPTFMEWDEFTFWGIAPKVVKSTGRLYTLEPGSMVGVTYVPGLVMLDQAFQFLGA
                     AFVPWKVFAAYDLLYFAVFAAALSMFRRRGWRMAVPMAVMLTLLPYLLTIWQRDIYVQ
                     PIYMSSYADIPMGLLFGAGLVLYFAAGKKTPAVLAAAALAVTAESVAKDMGFALCLVA
                     VALICFDLLFVERGEVTFARLRGVWAKLCWCALTVLAPLGAFFGWALHMQAALGLSRF
                     DLGGSEEMGMVEMVATGLVELVSPQKSEKFAQIADGMYKAFFTTRLSMFGSGLAVVLV
                     ILALLALAYLCGDRVQKARTAWFAALSTLGFAAFYIFNIFTYAYIFKGQEALGLSNYN
                     RYIYPYYIGWMLAALALLGKSLADGRAQRAGGRALLWQGALFAAPYALMAAVLLPLRG
                     AWAAQDGASYRL"
BASE COUNT          734 a         1582 c         1584 g          951 t
ORIGIN      
        1 gcaccgccac caccgagacc acggccaggg ccatcaggcg ctttttgcgg gccccctttg
       61 ccgccccgat gcgccgggcc atgaggtagg tgaaaagcac gtgcagcgcc agcacagcgg
      121 cggagagcgg atggccgccc aggccaaagc cgtagaagag gcagctcgac agcagcagca
      181 ccgcgttttg caggcgcccc ggcgccagat aataggccag cgccaccagg ggcaaaaaca
      241 gcgcatagcc tgcggaggtg agggtcatgg aaacgctcct ttcaaggcgg cggccgagcc
      301 gcccggacac tgtgattttg ggcaaaaaca aagcagacca tccctccgcc tgcccggcgg
      361 aaagacgaaa tctgcgaaaa aatgcagcaa aatgccgctg aggtacgttc tattataaag
      421 gaagcgcccc ggcttgtaaa gcggccgggc cgccccgcag cccggctttt tattgatatt
      481 ggggccgttt tatagtatac ttaactatac ctgttgccct gcgtattttg gcagaaaatg
      541 aaaaaagaaa cgccccgcct gcggggcggc aagagaggga tcgcactatg tcgcacactg
      601 tcatggtcac cggcgccgac ggattcatcg gcagccatct caccgaggag ctcgtccgca
      661 gcggcgagcg ggtgaaagcg ttctgctatt acaactcttt caactcctgc ggatggatcg
      721 acaccctgcc gcccgagatc cgggacgaga tcgagatttt catgggcgat atccgcgacc
      781 ccaacggggt gcgcaccgcc atggccgggc aggagcgggt ctatcatctg gcggccctca
      841 tcgccatccc gttcagctac cactcgcccg acagctatgt ggacaccaat atcaagggca
      901 ccctcaacgt gctgcaggcg gcccgcgacc tgggcaccca gcggctgatg atcacctcca
      961 ccagcgaggt gtacggcacc gcccggtacg tgcccatcga cgagcatcac ccctatcagg
     1021 gccagagccc ctattccgcc accaagatcg gcgccgaccg gctggccgag agcttttacc
     1081 gcagcttcga tctgccggtg tccatcgtgc ggccgttcaa cgcctacggg ccgcgccagt
     1141 cggcgcgggc ggtcatcccc accatcatca tccagctgct ggccgggcgc accgagatcc
     1201 gcctgggcaa cctcaccgcc acgcgggact tcaactacgt caaggacacc gccgcgggct
     1261 ttatggccat cgccgactgc gcggaggcgg tggggcagga gatcaatatc gccaccgggc
     1321 gcgagatctc gatcggcgag ctggcggacg agctgatccg ccagatcaac ccggcggcaa
     1381 aggtggtctg cgaggagcag cggctgcgcc ccgccaagag cgaggtggag cggctgctgg
     1441 gcgacgccac caagctgcgc cagatgaccg gctgggcgcc ccgctacacc tttgaagaag
     1501 gcctggccga gaccatcgcc tttttgcgcg gccacctgga cagctacaag atcgacgcct
     1561 ataatctgta aaaaacggcg cgccccggcg ggcgcgcggg ctgggagtgc ggaatggaca
     1621 aagcgctgca aaaactgaag agcctttggg gccgcgcctg ggtgcgctgg gcgctgggct
     1681 atctctttgt gctgctgtgc ggcgtgggcg tggtgtatta ctggcgggtg gcttacggcc
     1741 ttgacagcct ctcgccctgg gtggcgaccg ccgtggtctg cggcggtttt tcggcggtct
     1801 acgcggtggt gtttctgatc gcgcattttg tcaggcagaa cctggcgctc aaggcctcgc
     1861 tgctggtgct ggtggcgggg ctctgcttcg tgttcgccaa cccgcctttg caggcgccgg
     1921 acgagaacat gcactttctg cgcgcctact cgatcggcag cggcaatttt ttgttcgagc
     1981 aaaatgagga ttaccccgac gacgtggacc tgctgatcca ggattttccc ggcctgtaca
     2041 accacgagct gatcgccacc gggcaagcca ccatggccga cggctttgcg cgctaccgcg
     2101 cccacctgca gcagggggcc accgccccca acgccgccac cctgatccag cagctggtgc
     2161 cctatctggc ccaggcgccg gccatcgccg cggcgcggct cttcggcggc gacgcgctgg
     2221 cctgcatgta tgccgcgcgc gcgggcaatt tgctgctcta cgccgcgctg tgctatgtcg
     2281 ctttccgggc ggcaaagcgg tttttgccca tcctcatcac ccttgccatc tcgcccatcg
     2341 ctctgttcac cgccgggtcc tgcagcgccg acgggctgct gctgggcctg acctgggtgt
     2401 tcatcgggtt ctgcttttcc gaccggctct cccgggggca ggtcgcggcg ctggcggtca
     2461 gctttgggct gatggttttt gccaagtata ccccggtggc tatgctgccg ctggtggcgc
     2521 tcatcccgca gcccgagggc ctgcgggcgg gccggggcaa aacggcccgc agccgctggc
     2581 tggtcgcggc agcctgcctt gcgggggcgg cgctggtcta tgcattgctc accgcctaca
     2641 ccggctttgc cagcaattac ggggccctgc cctatgcgga caccggtgtg cgcccgggcg
     2701 aacagctgcg gttcatcctg tccaatccgc cccgctatct ggcggtcttc ctctactcgc
     2761 tctaccgcga caaggcgaac ctgtctatgc tgggcaattt cggctggatg gatatgaacg
     2821 tgccctttgt cagtgtcttt tcgccgttgg tgctgctctt ttcagccggc atgtcggcgc
     2881 tggagggcgc gcgggaaaag ccgcgcaccg gctgggcatg cgccctcacg gccgcgctgc
     2941 tgtacgcgtt cacctatgcg ggcatgtatc tgaccagcac cccggtctcg ctgcccgaga
     3001 tcaacggcgt gcagagccgc tatctgctgg gggccttttt cgcgctgctg gtgctggccg
     3061 ccatgctgct gggccgcacc atggcgctgg acaagctgcg ccccggccag ccgcagaaga
     3121 ccccgcccgc ctggcggatg ctgcatctgg cgttctgttt ttcgctggtt tcggccctgc
     3181 tgctgttcca atcctactat atcggcccct gaaacgggcc ctttgtcctt tgcccgcgcc
     3241 gcggccctgc gccgcggccg gagaccccaa tctgcgggag gataacctga tttgacggca
     3301 ttcattgaga tgttcatcac tttcgcggcg cttgcggccc tgtgcctttt tgtgggcgcg
     3361 cgcaccagcc tgccctcggg cgcggccccg ctggccgtgc tctgcgccac catgctctgg
     3421 tacagcctga tgggctgttt tgacctgctg ttcccggccg ggctgctctg gtttgccgcc
     3481 gccctggccg ctgccgccgc cctggcgctg cggcgcaaag agatccgatg gaaagagctg
     3541 ttcggccccg gcatggtctt ttttctgatt gccgggctgg tggtgatcgg cctgttcgcc
     3601 ctgcggcagc ccacctttat ggagtgggac gagttcacct tttggggcat cgcgcccaag
     3661 gtggttaagt ccaccgggcg gctgtacacg ctggagccgg gcagcatggt gggggtcacc
     3721 tatgtgcccg ggctggtgat gctggaccag gcgttccagt ttctcggggc ggcctttgtg
     3781 ccctggaagg tctttgccgc ctatgatctt ctctatttcg ccgtcttcgc cgcggcgctg
     3841 tccatgttcc ggcggcgcgg ctggcgcatg gcggtgccga tggcggtgat gctgaccctg
     3901 ctgccctacc tgcttaccat atggcagcgg gacatctatg tgcagcccat ttacatgagc
     3961 tcctatgccg acatccccat ggggctgctg ttcggggccg ggctggtgct gtatttcgcg
     4021 gcgggaaaaa agacgccggc cgtgctggcc gcggcggcgc tggccgtgac ggcggagagc
     4081 gtcgccaagg atatgggctt tgccctctgc ctggtggcgg tggcgctgat ctgcttcgac
     4141 ctgctctttg tggaaagggg cgaggtcacc tttgcccggc tgcgcggcgt ttgggccaag
     4201 ctctgctggt gcgcgctgac cgtgctggcg ccgctggggg cctttttcgg ctgggcgctg
     4261 cacatgcagg cggctttggg cctcagccgg ttcgaccttg gcggcagcga ggagatgggc
     4321 atggtggaga tggtcgccac cggccttgtc gagcttgtca gcccgcagaa gagtgaaaag
     4381 ttcgcccaga tcgcggacgg catgtacaag gcgtttttca ccaccaggct gagcatgttc
     4441 ggcagcggcc tggcggtggt gctggtgatc ctggcgctgc tggcgctggc ttacctctgc
     4501 ggcgaccggg tccaaaaagc gcgcaccgcg tggttcgcgg cgctttccac actaggcttt
     4561 gcggcgtttt acatctttaa tatcttcacc tacgcctata tcttcaaggg gcaggaggcc
     4621 ctgggcctga gcaactacaa ccgctatatc tacccctatt acatcggctg gatgctggcc
     4681 gccctggcgc tgctgggcaa gagcctggcg gacgggcgcg cccagcgggc ggggggccgc
     4741 gccttgctgt ggcagggcgc cctcttcgcg gcgccctatg cgctgatggc ggccgtgctg
     4801 ctgcccctgc gcggcgcctg ggccgcgcag gacggggcct cttatcggct g
//
LOCUS       DBMDYA010000135         4824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4277_length_4824_cov_1.558543, whole genome shotgun sequence.
ACCESSION   DBMDYA010000135 DBMDYA010000000
VERSION     DBMDYA010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_4824_cov_1.558543"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="ACZPFK_04590"
     CDS             <1..697
                     /locus_tag="ACZPFK_04590"
                     /inference="COORDINATES: protein motif:HMM:NF039826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR1405621.1"
                     /translation="APHFAPERCIAAPFARHPVCGPTPGLALAADALLLLSWYKLRDD
                     LADEGAPRRVFSRTACLTLKGAYGEAARRRPALDALFCRCMERQAALEAAGCALPDEA
                     ASPSAELLGGLFAECAAEEDQRLVLERYGLFLGRILYFLDAAEDFERDAAQGRYNVFL
                     QAGVCREEMLRQARALCNMCAGEATMCYHLLPLKENRPLLDNVMYLGLPQSILRIGES
                     QKDKRRNRHERPL"
     gene            681..1274
                     /locus_tag="ACZPFK_04595"
     CDS             681..1274
                     /locus_tag="ACZPFK_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR1405622.1"
                     /translation="MRDPYEVLGLRPGATEEEIKAAYKALAQKYSAENYAASPLEGEA
                     GRQMAEINEAFDALMGSLRTGAGPGAFGGKDGRYEEIRRLINEGKADEALRRLQEKDE
                     SAVSGEWNFLCASAYYYKGWLGQALPYFEKAASLCPDNREYAAACRRLKNSAAGNVAG
                     NPYAGTPQGVAGCSCCDMCTALMCMDLCCGFGRGGCC"
     gene            1276..1818
                     /locus_tag="ACZPFK_04600"
     CDS             1276..1818
                     /locus_tag="ACZPFK_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405623.1"
                     /translation="MHSQTKRVALCGVFAALSTVVMFLGGMLPLATFTAPAIAGLLLW
                     PVALEFNLQSGLMAWAVVSALSFFIVPDKEMSLIFIFLLGYYPMLKGKLDKIHKKAIQ
                     WLAKMTAFNLSVFVMYSLILFVFPIGEIVEEFSGSSHLFLAGLVLLGNFAFIVYDFAL
                     VKIRLVYYYRLRPMVFGRGR"
     gene            2291..4111
                     /gene="glmS"
                     /locus_tag="ACZPFK_04605"
     CDS             2291..4111
                     /gene="glmS"
                     /locus_tag="ACZPFK_04605"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1405624.1"
                     /translation="MCGIVGYVGRRQAAEVLIDCLSKLEYRGYDSAGVALFEGSEIEV
                     VKATGRLKILAEKLRERAPIESRCGIGHTRWATHGAPSDVNSHPQSSSRISIVHNGII
                     ENYLKLKEFLLQNGYTFESETDTEVLVKLLDYHYDGDPLSTIIKTLSQVRGSYGLAIL
                     FRDFPGRIYAVRRESPLIVGYGDEENFLASDIPALLKYTREYSILEEGEIAEIDEAAI
                     RVYNEFGKPVEKERLTADWDVEAAEKGGYAHFMLKEIHEQPSAMASTINSRLENGLPQ
                     LHLPELTDEALRGVERIHIVACGTAMHAGMVGKTAIERLARVPVEVDIASEFRYRDPL
                     VGKNDLVIIVSQSGETLDTLAALKLAKEKGARTLAIVNVVGSSIARAADDVLYTYAGP
                     EIAVASTKAYSVQLAVLYVFALRLALARGTLAEAEARRYVNELTRLPELLEGLLADTE
                     HIKYLASLFMNCEDLFYLGRGFDYALSLEGSLKLKEISYIHSEAYAAGELKHGTISLI
                     VEGTPVIALATQDAVFEKTISNVKETKSRGARVILFCKEGVEVPGDVADYVVKLPRYE
                     ELFMPMLSVVPLQLFAYYMAVLRGCDVDKPRNLAKSVTVE"
BASE COUNT          923 a         1443 c         1534 g          924 t
ORIGIN      
        1 ggccccgcac ttcgcgcccg agcgctgcat cgcagcgccc tttgcccggc accccgtctg
       61 cgggcccacc ccggggctcg ccctcgcggc ggatgcactg ctgctgctca gctggtacaa
      121 gctgcgggac gacctggcgg acgagggcgc gccgcggcgc gttttttccc gcaccgcctg
      181 cctcaccctc aagggcgcct atggcgaagc ggcgcggcgg cgcccggcgc tggatgcgct
      241 gttctgccgc tgcatggaac gtcaggccgc gctggaggcc gccggatgcg cgctgcccga
      301 cgaggccgca tcccccagcg ccgagctgct gggcggcctg ttcgcggaat gcgccgcaga
      361 agaggaccag cgccttgtgc tggagcggta tgggctcttt ttgggccgca tcctttattt
      421 tctcgacgcc gcggaggatt ttgagcgcga cgccgcgcag gggcgctaca atgtatttct
      481 gcaagcgggc gtctgccgcg aagagatgct gcgccaggcg cgggcgctgt gcaatatgtg
      541 cgccggggaa gcgacgatgt gttatcatct tctaccgctc aaggaaaacc ggccgctgct
      601 ggacaacgtg atgtatctcg ggctgccgca aagcatcctg aggatcgggg aaagtcaaaa
      661 ggacaaaagg agaaaccgtc atgagagacc cctatgaggt gctgggcctg cggcccggcg
      721 ctacggaaga ggagatcaaa gccgcctata aggccctggc gcagaaatac agcgctgaaa
      781 actatgccgc ctcgcccctg gagggggaag ccgggcggca gatggcggag atcaacgagg
      841 cctttgacgc tttgatgggg tcgctgcgca ccggcgcggg cccgggcgct ttcggcggca
      901 aggacggccg ctatgaggag atccgccgcc tgatcaacga gggaaaggcg gacgaggcgc
      961 tgcgcagatt gcaggagaag gacgagagcg cggtgagcgg cgagtggaac tttttgtgtg
     1021 ccagcgccta ttattataag gggtggctgg gccaggcgct gccctatttt gaaaaggccg
     1081 cgtccctctg ccccgacaac cgcgaatacg ccgccgcctg ccgccggctg aaaaattccg
     1141 cagcgggcaa cgtggcgggc aacccctatg cggggacgcc gcagggcgtg gccggctgca
     1201 gctgctgcga tatgtgcacg gcgctgatgt gcatggacct ctgctgcggg tttgggcggg
     1261 gaggctgctg ctgagatgca ttcgcaaacc aagcgggtcg ccctgtgcgg tgtttttgcc
     1321 gctctgtcca ccgtggtcat gtttttgggc ggcatgctgc cgctggccac gttcaccgca
     1381 cccgccattg cagggctgct gctgtggccg gtggcgctgg agttcaacct gcagagcggg
     1441 ctgatggcat gggcggtggt gagcgccctc tcctttttca tcgtgcccga caaagagatg
     1501 agcctgatct ttatttttct tctcgggtat taccccatgt taaagggaaa acttgacaaa
     1561 atccacaaaa aggcgataca atggctggca aagatgacgg cgttcaatct gtcggtcttt
     1621 gtgatgtaca gcctgatcct gttcgtgttt cctatcggcg agatcgtcga ggagttcagc
     1681 ggctcctcac acctgttcct ggcggggctg gtgctgctcg gcaacttcgc ctttatcgta
     1741 tatgactttg ctctggtgaa aatcaggctg gtctactatt accggctgcg ccccatggtc
     1801 tttggccggg gcagatagga gagcgccgga aatgccgtaa acggcaaagg aggtttgatc
     1861 gttatgctgc cccaagcgcc gggccccagc tcagcaaaga gcctggggtg acgaggtgcg
     1921 aagtttgcgg ggacgttttt tcagcggcgg ctttgccacc ggctgtcaaa cggcctccat
     1981 cgaaagattc ggcggatgct tcgccggccc tgttcccgga tgggtcgtga gaagatgtgc
     2041 aaaaaacggc agcaatgccg caacagagac gtcttcacgg gatgaaaatg gacttttgca
     2101 gcggccgcag cgccctgcag cttgataaag accgcgcgcc gcatcacggg gcgcacggtc
     2161 aaaagccgcc gagacggctt ttttcaaaaa ctgaaaagac ggacgacatg tggtatctcc
     2221 cccttgggac tacccctggt tttgttttgc cttttcagcc catactgcgc ttgataaagg
     2281 agattttttt atgtgtggaa ttgtaggcta tgtgggccgc cgccaggccg cggaggtctt
     2341 gatcgattgt ctgagcaaat tggaataccg cgggtacgac tctgcgggcg tcgccctgtt
     2401 cgagggcagt gagatcgagg tggtcaaggc caccggccgc ctgaaaatat tggccgaaaa
     2461 gctgcgcgag cgggcgccca tcgaatcccg ctgcggcatc ggtcacaccc gttgggccac
     2521 ccacggcgcc ccatccgatg tcaacagcca tccccagtcc agctcgcgca tcagcatcgt
     2581 gcacaacggc atcatcgaaa actatctcaa gctcaaagaa tttttgctgc aaaacggcta
     2641 cacctttgaa agcgagaccg acacggaggt gctggtaaag ctgctggact atcactatga
     2701 cggcgacccg ctctccacga tcatcaagac tctgtcccag gtgcgcggca gctacggcct
     2761 tgccatcctc ttccgcgatt ttcccggccg catctatgcc gtccgcaggg agagcccgct
     2821 gatcgtgggc tacggcgacg aagaaaactt tctcgcctcc gacatcccgg cgctgctgaa
     2881 atacacgcgg gagtacagca ttttggagga gggcgagatc gcagagatcg acgaggcggc
     2941 aatccgcgtc tacaacgagt tcggaaagcc ggtggaaaaa gagcgcctca ccgccgactg
     3001 ggacgtggaa gccgcggaaa agggcggtta cgctcatttc atgctcaagg agatccacga
     3061 acagcccagc gccatggcca gcaccatcaa ctcgcggctg gaaaacgggc tgccccagct
     3121 tcacctgccc gagctgaccg atgaggctct gcgcggcgtc gagcgcatcc acatcgtggc
     3181 ctgcggcacc gccatgcatg cgggtatggt gggcaagacc gccatcgagc ggctggcccg
     3241 ggtgccggtg gaggtggata tcgcctccga gttccgctat cgggaccccc ttgtgggcaa
     3301 aaacgacctg gtgatcatcg tctcccagag cggcgagacg ctggacacgc tggccgcgct
     3361 caagctggcc aaagaaaaag gggccaggac gctcgccatc gtcaatgtgg tcggctcatc
     3421 catcgcgcgc gcggcggacg atgtgctgta cacctatgcc ggcccggaga tcgccgtggc
     3481 gagcaccaag gcctacagcg tccagctggc ggtgctgtat gtctttgccc tgcggctggc
     3541 cctggcccgc ggcaccctgg cggaggcgga ggcgcgccgc tatgtaaacg agctcacccg
     3601 cctgcccgag ctgctggagg ggctgctggc ggacaccgag cacatcaaat atttggcctc
     3661 gctttttatg aactgcgagg acctgttcta tctgggacgc ggctttgact atgcgctgag
     3721 cctggagggc tcgctgaagc tgaaagagat cagctacatc cactcggagg cctatgcggc
     3781 gggggaactc aagcacggca ccatcagcct gatcgtcgag ggcacgccgg tcatcgccct
     3841 cgccacccag gacgcggtct ttgaaaagac gatcagcaac gtgaaagaga ccaagagccg
     3901 gggcgcccgg gtgatcctgt tctgcaagga gggcgtggag gtgcccgggg acgtggcgga
     3961 ctatgtggtg aagctgcccc gctatgaaga gcttttcatg cccatgctgt cggtggtgcc
     4021 cctgcagctt ttcgcctact atatggcggt cctgcggggc tgcgacgtcg acaagccgcg
     4081 caatctggca aagagcgtga cggtggagtg agctccggcc ccgcgcggcg gaagtcgccc
     4141 cacccactct cacggcccac gccgcaaaag acgctccggt gaggcgggca tcccacacca
     4201 gctctgcaaa acagctttgc cggcgggcgc tttgccctgc cggcattttt tgccctgtcc
     4261 ctcaagagca gagctcttcg caaaagagcg ttgctccggg aggaaaattt gtgtgcggag
     4321 catcgtcgga tccctccgcc cgcaagtggt tttgtgattt cctcctgtgg cgtgccggcg
     4381 cgagcagatg ggtaaaatcg gcgcaggggt tgcggctttc ccctgcgggg cggcagagac
     4441 ggcagttttg aactgacggt ttcggcacag ttcccccgca gctattttaa agaagaatgc
     4501 ggctgctgca tttttgcaac agccgcattc ttctttgctc acagaggcct ctgttttttc
     4561 aaagccggtc acagaccttt gagggcggcc gctctccccc aatcctgccg cggcaagagc
     4621 cgcttttggc tgcgcacgct ttcaagctgt tctcactaag gcgggacaaa agagcgctga
     4681 agcgccctgc tgcacgaaaa atcacctcaa aaataaaatg taaagcaatt taacttttca
     4741 aagaaagctt ttcaggacct cctccacccg gcggcaggtg tgggtggcct gcgctttcac
     4801 gcccggggcg gccccttcca tcgc
//
LOCUS       DBMDYA010000136         4804 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4294_length_4804_cov_1.437593, whole genome shotgun sequence.
ACCESSION   DBMDYA010000136 DBMDYA010000000
VERSION     DBMDYA010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_4804_cov_1.437593"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(54..341)
                     /locus_tag="ACZPFK_04610"
     CDS             complement(54..341)
                     /locus_tag="ACZPFK_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405625.1"
                     /translation="MELTAILTGRKAPGKEVGTREDAAFIERYGAADTVGQLKECEYL
                     LRKNEMLFNMESDDELIDCRIYEREALLARYQYLLRRVRAERAAAKTASAG"
     gene            520..1494
                     /locus_tag="ACZPFK_04615"
     CDS             520..1494
                     /locus_tag="ACZPFK_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014476.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR1405626.1"
                     /translation="MKTRIVRGEEVRRLLTMEECIEAMRTALRDVSSGDGTMLQRGML
                     PLPADNKFALMGGADLQTGLCGAKVIVFPGAEAARRRTSQGVIPLFDCKTGGLAAIVD
                     AEQITAVRTAAASAVATGLLARKSASSLAILGAGRIGRMHIEAIRLVRPIETVYIWNR
                     TLARAQECCRQAAGLGLRAVLCTSAEEAVRAADIVCTVTQAREPVLLGRWLKPGAHVN
                     AVGACSPMARELDDEAVLRSCVYADQREAALRDSGDLAIPLKAGTWSAQAICGEVGQV
                     LSGELPGRRSEEEITLFESVGISLEDLCAAALVFRKAEQAGLGLEVEF"
     gene            complement(1987..3384)
                     /locus_tag="ACZPFK_04620"
     CDS             complement(1987..3384)
                     /locus_tag="ACZPFK_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405627.1"
                     /translation="MAQKKIRTKEELFETYSIPKAVLVLSVPTVLSSLVTVLYSLADT
                     YFVGMVNDPIQTASVTLAAPVLLAFNAVNNLFGVGSSSMMSRAMGRRDGETVRRSSAF
                     GFYGALICGAVFALLCTVLRTPLLGLLGAGAETAAPTSAYLNWTVSLGAVPAILNVVM
                     AYMIRSEGAALHASVGTMSGCLLNIVLDPIFILPWGLDMKAEGAALATFLSNCVALAY
                     FLVYLYLRRRSTSVSISPRDFKLKRQIVSGVFAVGVPASVQNLLNVTGSMIMNNLAAV
                     YGAEALAAMGICTKINMVPMYVVMGVAQGIMPLISYNYASGNIPRMKKAFFFTARIGV
                     TISALASAFLFACADGLVEMFIANTATVAYGIRFLRGFCLALPLLCVDFLAVGTFQAV
                     GMGRESLFFAIARKLALELPLLYLLNAVFPLYGIPYAQPAAEAVLAVTACVVLSRFFR
                     RAEREAGGVAERIKG"
     gene            4458..>4804
                     /locus_tag="ACZPFK_04625"
     CDS             4458..>4804
                     /locus_tag="ACZPFK_04625"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1405628.1"
                     /translation="MKTLHCSALVWPSPVGPLTLVEENEALTGLHFGALPPAGAETNP
                     HPLLREAVRQLEEYFEGQRKEFDLPLAARGTPFQLRVWAALRQIPYGETRTYADIAAA
                     VGCPRGFRAVGMA"
BASE COUNT          914 a         1587 c         1418 g          885 t
ORIGIN      
        1 cgcggccggt ggacgatcct tgtgtattgc gcttccccag agggacggcc tctttaaccc
       61 gcgcttgccg tttttgccgc cgcgcgctcg gcgcgcaccc ggcgcagcag gtactggtat
      121 cgcgccagca gcgcctcccg ctcgtagatg cggcagtcga tcagctcgtc gtcgctctcc
      181 atgttgaaca gcatctcgtt tttgcgcagc agatattcgc actccttgag ctgccccacc
      241 gtgtcggcgg cgccatagcg ctcgataaag gccgcgtcct cccttgttcc gacttccttg
      301 ccgggggctt tgcgccccgt caggatcgca gtcagttcca atcgctccac ctcgctttca
      361 gtctatgtgg caaggatggg cagctagaac cgcttttaat ccacctgaaa gattttttcc
      421 gccggcaggt tggccgccgc gcggcgggag gagtataatg gagtggaaag ccggggcgtc
      481 ccccgccccg acgtcttttc tgcaaaggag gtctattgta tgaagactcg catcgtccgc
      541 ggcgaggagg tgcgccggct gctcaccatg gaggagtgta tcgaagccat gcgcaccgcg
      601 ctgcgggacg tttcctcagg ggacggcacc atgctgcagc gcggcatgct gcctctgccg
      661 gcagacaata aattcgccct catgggaggg gccgacctgc agacggggct ctgcggcgcc
      721 aaggtgatcg tcttccccgg cgccgaggcc gcgcggcggc gcaccagcca gggcgtcatc
      781 ccgctttttg actgcaagac gggcggcctt gccgccatcg tggacgccga gcagatcacc
      841 gccgtgcgca ccgccgccgc cagcgcggtc gccaccggcc tgctggcccg caaaagcgcc
      901 tcttccctgg ccatcctggg cgccggccgc atcgggcgca tgcacatcga ggccatccgc
      961 ctggtgcgcc ctattgagac agtctatatc tggaaccgca ccctcgcccg cgcgcaggag
     1021 tgctgccgcc aagccgccgg gctggggctg cgggcggtcc tttgcacctc ggcggaggag
     1081 gcggtcagag cggcggatat cgtctgcacc gtcacccagg cccgtgagcc cgttctgctg
     1141 ggccgctggc tgaagccggg cgcgcatgtc aacgccgtcg gggcctgcag ccccatggcg
     1201 cgggagttgg acgacgaggc cgtactgcgc agctgcgtct acgccgacca gcgggaagca
     1261 gccctgcggg acagcggcga tcttgccatc cccctgaaag cgggcacgtg gagcgcgcag
     1321 gcgatctgcg gcgaggtggg acaggtgctg tccggcgagc tgccgggtcg tcggagcgaa
     1381 gaggagatca ccctttttga atcggtgggc atttcgctgg aggatctctg cgcggcggcg
     1441 ctggtcttcc gcaaggcgga gcaggccggt ctcggcctcg aggtggaatt ttaggccgtt
     1501 cccctttttc cacgccctcc gcgggcggat acggcctatt tcctcctacc cgccctgccg
     1561 actgctgtcc tccatctctc ctggcccggt ttgatccaag ccaggtaaag agggccccga
     1621 tgtgcagctc atccgcacat cagggccctc ttttctatcc tgtcgtccgt gctgtcgccg
     1681 ccggggcaga aaaagcgtct ttccgatttt aaaagcggga aaccgctgga ggccccattt
     1741 tcgccctctg cttctgcagt aaagggcagc ggacggccct ccccccaggg ccgaccgagg
     1801 gcggattttc cctcttcgcc gcttcgcccc cataggagat cagcggtaaa agtgggcggc
     1861 aatcccctct ttgccgccca cttttaccgc acggcggacc gaggcggtaa agcccgttcc
     1921 agtcggttcc ggctcacgtt cttgaaatgc aaacagatcc ggctgcgcac agcgggcggc
     1981 tgcgggttac ccttttatgc gctccgcgac gccccctgct tcccgctccg cccgccggaa
     2041 aaagcggctc agcaccacac aggcggtcac ggcgagcacc gcctccgccg ccggctgcgc
     2101 atagggaata ccgtacaggg ggaacactgc gttgaggagg tagagcagcg gcagctccag
     2161 cgccagcttg cgggcaatgg caaaaaacag ggattcccgc cccatgccca cagcctgaaa
     2221 ggtacccacg gccaaaaaat cgacgcagag cagcggcaat gccaggcaaa agccgcgcaa
     2281 aaagcggatg ccgtacgcca ccgtagccgt gttggcgatg aacatctcca ccagcccgtc
     2341 cgcgcaggca aacaaaaagg cggaggccag cgccgaaatc gtcaccccga tgcgggcggt
     2401 gaaaaaaaac gcttttttca tccgcgggat attgccgctg gcatagttgt agctgatcag
     2461 cggcatgatg ccctgtgcca cccccatcac cacatacatg ggcaccatgt tgatcttggt
     2521 gcagatgccc atggccgcca gcgcctcggc cccgtagacc gcggccagat tgttcatgat
     2581 catactgccg gtcacattga gcagattctg caccgaggcc ggcacgccca ccgcaaagac
     2641 gccggatacg atctgccgtt tcaacttgaa atcacgcggc gagatgctga cgctggtgct
     2701 gcgcctgcgg agatatagat agacaagaaa ataggccagc gccacgcagt tggacaaaaa
     2761 tgtggccagc gccgccccct ccgctttcat gtccagcccc cagggcagga tgaagatggg
     2821 gtcgagcacg atgttcaaca ggcagccgct catggtgccg acgctggcgt gcagcgcagc
     2881 gccctctgag cggatcatat aggccatcac cacgttcagg atggcgggca ccgcccccaa
     2941 cgaaacggtc caattcaggt aagcggaggt gggcgccgcc gtctcggccc ccgcccccag
     3001 caggcccaac agcggcgtgc gcagcacggt gcagagcagc gcgaatacag cgccgcagat
     3061 caaggcccca taaaagccga aagctgagct gcgccgcacg gtctcgccat ccctgcggcc
     3121 catggccctg ctcatcatgc tggaactgcc cacgccgaac aggttgttca cggcgttgaa
     3181 cgccagcagt accggcgccg ccagcgtgac cgaagcggtc tggatcgggt cgttgaccat
     3241 gccgacaaag taggtgtccg ccaggctgta aagcaccgtc accagcgagc tgagcaccgt
     3301 cggtaccgag agtaccagca ccgccttggg gatcgaatag gtctcaaaca gttcctcttt
     3361 tgtccgaatt ttcttttgcg ccattctaca caactctccc cgccattgtc ttgcgcctgc
     3421 gcgcccacat cggtataata aagaaacaga gcttgtcctt tcagagcttt cacccatctc
     3481 catcttgagc agacggagca cttttgccgc aaatggcaaa aggcccgttg aagccgcccc
     3541 gtctgctgcc gccttaccgc gacgccccag gcaggggcgt cgcgcgcgcc tttggttttt
     3601 tgcgggacaa aagggacccg ggcttttcac cgggttgcct ccccgtgctc tgcccaaaac
     3661 agccgccgca cggctcttct ctcacggcgg ttgccgcaca gcggcaaaag ggtccccccg
     3721 caccggtttg ttgaaatcag tgtccgagag ggcaaagctc agtttcatcc tccccgtcgc
     3781 cgttattttg ccgcagcgtt ttctctccca acgcccgttt ccagccgctc tgtcgcagct
     3841 gaaacgaaca gccgaaaaag cgtctctcgc tccgctgcgg cgattctgta ttgctccgca
     3901 ccaaaaagcg agttgtttga atggcgtcat ttttcgtcgc cgcgcagcag ccgaaacgga
     3961 cgtcccccca aaataacctg tgccaagcta taaaagcggc tatggggagc cgccgggccc
     4021 acagccttaa aatcttgagc gcccgtccga ggacgggcca catagccgaa aaagcagctt
     4081 tcgcttcacc gtggctattt tttattgctc cgcaataaaa aactttatct ctatggccac
     4141 ggccggagga ccggccggat ggccgcaaaa aatagcctgc ggctattttt tatcactccg
     4201 caataaaaaa acaactcatt tcaatggccc cggccagggg actggccgga tggccgcaaa
     4261 aacagcctgt ggctattttt tatcgctccg caataaaaaa cacctcattt taataacccc
     4321 ggccagggag accgaccgga tggccgccca aaacggcctg cggctatttt ttattctagc
     4381 atcctatccg ccgtccggca agggggagac cgcagctctt cctccgcgcc tgacaacgcc
     4441 gtcaaaaagg agaaagtatg aaaactctac attgctctgc tctggtatgg ccctctcctg
     4501 tggggccgct gactttggtg gaagagaacg aagcccttac cggcctgcat tttggcgcat
     4561 tgccgcccgc aggcgcagag accaacccgc atccgctgct aagagaagcc gtgcggcagc
     4621 tggaagaata tttcgagggc cagagaaaag agtttgactt gcctctggcg gcgcgcggca
     4681 cgccgtttca gctgcgggta tgggcggcgc tgcggcagat cccctacggg gagacccgca
     4741 cctatgccga catcgccgcc gctgtgggct gcccccgggg ctttcgcgcg gtgggcatgg
     4801 ccaa
//
LOCUS       DBMDYA010000137         4801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4296_length_4801_cov_1.331489, whole genome shotgun sequence.
ACCESSION   DBMDYA010000137 DBMDYA010000000
VERSION     DBMDYA010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_4801_cov_1.331489"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            37..627
                     /locus_tag="ACZPFK_04630"
     CDS             37..627
                     /locus_tag="ACZPFK_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405629.1"
                     /translation="MAELYYQGHGSFRITAADATTVYVDPFAGDGYDRPADLILVTHD
                     HHDHNRVDLVPRRPGCEVITFRQALAGGVYHSFDSHGVQVQAVKAANANHDPACCVGF
                     LLTVDGRTVYAAGDTSTTEEMKGLAARRLDWALLPIDGVYNMDPEEASRAARLIGAAH
                     TVPVHMKPGALFDEAMAARFRAPGKTVLRPGETAAL"
     gene            965..2866
                     /locus_tag="ACZPFK_04635"
     CDS             965..2866
                     /locus_tag="ACZPFK_04635"
                     /inference="COORDINATES: protein motif:HMM:NF018584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HIR1405630.1"
                     /translation="MKRLAAYFLVFFFVLGALPARAAGFARDFTERGELDAEALWESA
                     QVSDALWAEWEAIAGDPSGVLPAADIETVLSAMDALVTCTDLSAAHPWRARVLLRAAR
                     RAVALSDQRAPLVTGEADTRALWAYCRWHLAVMALTLGGRQEYEQELAAAAKLLAAYY
                     GQAGEIGTNPECDLIVEYLELLAGRGDREGARAYLAALLRLDRSRNLLVWIRPLDSRV
                     TAACGCPPEALLPEPLAFWTVDGLEWAAVPTTEEGGVPLVDLNRLAVQLGAVTGGLVG
                     GTVSGSLYDGSFEVREGETTATGLEGSLSLSAPPRLSRSAQGENALLVSAEDAALLLG
                     QRLVTRTLRVRLNSFDPGGERRVASFEPALRAALNDGQARLIESLSALPVTADLFSFE
                     DGGAELDAQKASALFASLCPLSGAARGPGWGEFCRYQLNAGSIQDKDGLLRAVKELER
                     RGLHASTTALTGGDAPAWFARKWHSRDPQGDLLAWDLCQAVQLVQYGYAAGLLSAGEA
                     LEIERRCAARAQGQFPDYDSYLDGWLMGLDVQLDGDARETESYPQLAQLCLALNGPDA
                     GFAAAWTTSLEGLPQAPGPSGPPPWVAPASAALLSAMLLAALTALSAVLLHNKKKRAR
                     ARRKGSFRL"
     gene            2863..>4801
                     /locus_tag="ACZPFK_04640"
     CDS             2863..>4801
                     /locus_tag="ACZPFK_04640"
                     /inference="COORDINATES: protein motif:HMM:NF018584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HIR1405631.1"
                     /translation="MTKRLLCGTLCALLLFAALLPALPPARAAGAGPLTGDALAEMEA
                     DAAADPLSADFEALWAGLSALAEREADDGWRVGHPQEAAGFDALAARADALCLSAESA
                     LSARYEEDYDVSAYLDGSALWLARALWQLARLDKAAANGSAYKDHLGRAGAVYYSCVV
                     QYGAANQALVPQVELDFDFVLAYCGYSGQIGGDGRGLINEMMGWEWEAEEQPFTDFYE
                     AHSEQIDELLADSGASAEKYPDRGWYSVDGDPWEMGEVVFDGETPYVALERYLRSYGG
                     KDLTDYSADLTGRRVRWLLCGDEIRADEETILLNGEAVELSAPPFWEGGLLYVTLYDA
                     DYLAFALSEQQSMEYYASPWDDAPVRGEVYVLRPYFGEQEPTEEQLLFLEGIGAGINL
                     QNLIGGDGSLSPADAPGLCEDYLCGWAGLTRGEANSLYAQSIWEQSWEVSGPEELVNN
                     YNWLLDEGHRGRFRVLANDSSAPAWFWQKWGARLKEHSFFAWDLARAVQLCQWGCCAG
                     YLPPEQAVSMAFEAARRLQAAFPSWQAFAEDYLMGYDLFLSEDADKADFYSLRRPILE
                     ALYADGAYRGVGWDLGLPAPSQEGPAAPALSGAGGVSAPEAELIRSVALACLGLQLVT
                     LIAGAAALYLSLLEPEDNYFFV"
BASE COUNT          692 a         1586 c         1703 g          820 t
ORIGIN      
        1 gtccacatcc tgcttttttc agaaaggggc gcaattatgg cagagctcta ttatcaggga
       61 cacggcagtt ttcgcatcac ggcggcggac gcgaccaccg tttatgtcga cccctttgcg
      121 ggggacggct atgaccggcc cgccgacctg atcctggtca cccacgacca tcacgaccac
      181 aaccgggtcg atcttgtgcc gcggcggccg ggctgcgagg tgatcacttt caggcaggcg
      241 ctggcaggag gcgtctacca cagctttgac agccacggcg tgcaggtgca ggcggtcaag
      301 gccgccaacg ccaaccatga ccccgcctgc tgcgtgggct ttctgctcac cgtggacggc
      361 cggacggttt atgccgccgg ggacacctcc accaccgagg agatgaaagg tctggcggcc
      421 cgccgactgg actgggcgct gctgcccatc gacggcgtgt acaacatgga cccggaggag
      481 gcctcccggg cggcgcggct gatcggggcc gcgcacacgg tgccggtgca tatgaagccc
      541 ggggccctgt tcgacgaggc gatggcggcg cgctttcgcg ccccgggcaa gacggtgctg
      601 cgcccggggg agacggcggc cctgtaaagg gaggccccgc ccctatacag cagcatcccc
      661 gccgtcgctg cggccgggcc tgtggttttc cgcgcgcgcc gggcatattc cactttttgt
      721 tttcctcgcg gcgcgggtcc gggtggaacc ccgcggcgag gcctgcaaaa agcccagcgc
      781 ccggccgccg ttttcaactc caccgagggc tctgccgggc agcccggcgc acgtgctgtc
      841 tcgccccaaa cccagcttgt ttttttgcgg tgtgccctgc gggcataccg cttttttcct
      901 ttttgtgcta tcataaaggc aaagtagttt caaccgggcg tcccgtgggg gggaggtggg
      961 ccgtgtgaag cgtttggccg catattttct ggtttttttc ttcgttttgg gcgcgcttcc
     1021 ggctcgggcg gcgggctttg cgcgggactt taccgagcgg ggcgagctgg acgccgaggc
     1081 gctgtgggag agtgcccagg tcagcgacgc cctgtgggcg gaatgggagg ccatagccgg
     1141 ggacccgtcg ggcgtcctgc ccgccgccga cattgaaacc gtcctctccg ccatggacgc
     1201 ccttgtcacc tgcaccgacc tgtcagccgc tcacccctgg cgggcccgcg tgctgctgcg
     1261 cgccgcccgg cgggcggttg cactcagcga tcaaagggct cctctcgtca caggggaagc
     1321 ggacacccgc gcgctctggg cctactgccg ctggcatctg gccgtgatgg ccctgaccct
     1381 gggcggccgg caggagtacg aacaagagct ggccgctgct gcaaagctgc tggccgctta
     1441 ttacgggcag gcgggtgaga tcggcaccaa cccggaatgc gatttgatcg tcgaatatct
     1501 ggagctgctg gccgggcgcg gtgaccggga gggtgcccgc gcctatctcg ccgccctgct
     1561 gcggctggac cgcagccgta atctgctggt ctggatccgc ccgctggaca gccgggtcac
     1621 cgccgcctgc ggttgcccgc cggaagcgct cttgccggag cctctggcct tttggacggt
     1681 ggacggcctg gaatgggccg ccgtccccac caccgaggag ggcggcgtgc ctctggtgga
     1741 cctaaaccgg ctcgccgtcc aactgggggc ggtaacgggc ggcctcgtcg gcggcacggt
     1801 gagcggcagc ctttacgacg gctcttttga ggtgcgcgag ggtgaaacca ctgccaccgg
     1861 gctggagggc agcctttccc tgtcggcgcc gccgcgactg agccgttcag cgcaggggga
     1921 gaacgcgctg ctggtctccg ccgaggacgc cgcgcttttg ctgggccagc ggctggtcac
     1981 ccgcacgctg cgggtacggc tgaacagctt tgatccggga ggggaacgcc gggtggccag
     2041 ctttgagccc gccctccgcg ccgccctgaa cgacgggcag gcgcggctga tagagagcct
     2101 ttccgccctg cccgtgacgg cggacctgtt ctcttttgaa gacggcggag cagagctgga
     2161 cgcgcagaaa gcctctgccc ttttcgcctc tctttgcccc ttaagcggcg cggcgcgcgg
     2221 cccgggctgg ggggaattct gccgttacca gctgaacgcc gggagcatcc aggacaagga
     2281 cggcctgctg cgcgcggtga aagagctgga gcgccggggc ctgcacgcct ccacgacggc
     2341 gttgaccggc ggggacgccc cggcctggtt cgcccgcaaa tggcacagcc gggacccgca
     2401 gggcgacctg ctggcctggg atctctgcca ggctgtacag ctggtccagt acggatacgc
     2461 cgcgggcctg ctcagtgccg gagaggcgct ggagatcgaa cggcgctgtg ccgcccgggc
     2521 gcaggggcaa ttccccgatt atgacagcta cctggacggc tggttgatgg ggctggacgt
     2581 ccagttggac ggcgacgccc gcgagacgga aagttatccc cagctggcgc agctttgcct
     2641 cgccctcaac ggcccggacg cgggctttgc cgccgcctgg accacctcgc tggagggcct
     2701 gccccaggcc ccaggcccaa gcggccctcc gccgtgggtc gcgcctgcct cggcggcgct
     2761 gctttcggcg atgctgctgg ccgccctgac ggcactgtcg gctgtgctgc tgcacaataa
     2821 aaaaaagcgg gcccgcgccc gccggaaagg gagtttccgc ctatgacaaa acgcctgctt
     2881 tgcggcacgc tctgcgcgct gctgctgttc gccgccctgc tcccggccct gccgcccgca
     2941 cgggccgccg gggccgggcc gctgaccggc gacgccctcg cagagatgga ggccgacgct
     3001 gcggccgacc cgctctcggc ggactttgaa gcgctctggg ccggcctttc cgccctggcc
     3061 gagcgggaag ccgacgacgg ctggcgggtc ggacacccac aggaggccgc cggcttcgac
     3121 gcgctcgccg cccgggcgga cgcgctctgc ctctcggcgg agagcgcgct cagcgcccgc
     3181 tatgaggagg attacgacgt ctcagcctat ctggacggct ccgccctctg gctggcccgc
     3241 gcactgtggc agctggcccg gctggacaag gccgcggcca acggcagcgc ctataaggac
     3301 catctagggc gcgcgggcgc cgtctattac agctgtgtgg tacagtacgg ggcggccaat
     3361 caggcgctgg tgccccaggt ggagctggac tttgattttg ttctcgccta ctgcggctac
     3421 tcggggcaga taggtgggga cggccgcggc ctgatcaacg agatgatggg ctgggaatgg
     3481 gaggcggagg aacagccttt cacggatttt tatgaggcgc acagcgaaca gatcgacgag
     3541 ctgctggccg acagcggcgc ctcggccgaa aagtaccccg accggggctg gtactcggtg
     3601 gacggcgacc cctgggagat gggggaggtc gtctttgacg gcgagacccc ctacgtggcc
     3661 ctggagcggt atctgcgctc ttacggcggc aaggacctga ccgactacag cgccgacctc
     3721 accggccgcc gggtgcgctg gctgctgtgc ggcgacgaaa tccgggcgga cgaggaaacg
     3781 atcctgctga acggcgaggc cgtggagctc tcggccccgc ctttctggga gggcggcctg
     3841 ctctatgtca ccctttacga cgcggactat ctggctttcg cgctgtcgga gcagcagagc
     3901 atggaatact atgcctcccc ctgggacgac gcgccggtgc gcggggaggt gtatgtgctg
     3961 cggccctatt tcggcgagca ggagccgacg gaggaacaac tgctcttttt ggagggcatc
     4021 ggcgcgggca tcaacctgca aaacctgatc gggggcgacg gcagcctctc ccccgccgac
     4081 gcgcccgggc tctgtgagga ctatctctgc ggctgggccg ggctcacccg cggcgaggcc
     4141 aacagcctgt atgcccaatc catttgggag cagagctggg aggtgagcgg gcccgaggag
     4201 ctggtgaaca actacaactg gctgctggac gaggggcacc gggggcgctt tcgcgtgctg
     4261 gcaaacgatt cttccgcgcc ggcgtggttc tggcaaaaat ggggcgctcg gctcaaagag
     4321 cacagctttt tcgcctggga tctggcgcgg gcggtgcagc tctgccagtg gggctgctgc
     4381 gcgggctacc tgcccccgga gcaggcggtc tcgatggcct ttgaggcggc gcggcggctg
     4441 caggcggcgt ttccctcctg gcaggccttt gccgaggatt acctgatggg gtacgacctc
     4501 tttctctccg aggatgcgga caaggccgat ttttatagcc tgcggcgtcc catcctggaa
     4561 gcgctttacg cggatggcgc ttaccggggc gtgggctggg atctggggct gcccgccccg
     4621 tcacaggagg gcccggcggc cccggccctc tccggcgccg gcggcgtgtc cgcgccggaa
     4681 gcggagctga tccgcagcgt ggcgctcgcc tgcctgggac tgcagctggt aaccctgatc
     4741 gccggcgcgg ccgcgctgta cctgagcctg ctggagccgg aggataacta cttctttgtt
     4801 c
//
LOCUS       DBMDYA010000138         4769 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4326_length_4769_cov_1.433376, whole genome shotgun sequence.
ACCESSION   DBMDYA010000138 DBMDYA010000000
VERSION     DBMDYA010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_4769_cov_1.433376"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="ACZPFK_04645"
     CDS             <1..750
                     /locus_tag="ACZPFK_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR1405632.1"
                     /translation="EKCGILKPGCVAVSYPGQRPGAAAVIARRCEELGVPLRLPDPQD
                     IKLLWETLRENHVDYGGYEVRLRLHGSHQAANCALAIEAALGLCDQGYDIPDEAILSG
                     LERASLPARAEVLSLHPPVLLDGAHNPDSAAALAALLKTTRTPPLTAVLGMLKGKDAE
                     GVLKALEGCFDQVYTVAPDTPRAVSADLLAAAASEHFRRVEACGSLAQALAKAEARGG
                     GFCVCGSFYLAGEARRLLGEGGKLSSGLDSL"
     gene            937..1260
                     /locus_tag="ACZPFK_04650"
     CDS             937..1260
                     /locus_tag="ACZPFK_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR1405633.1"
                     /translation="MAECRLEQSGPVLVAYLSGEIDHHAAGRLREQIDARILSASPER
                     VVLDFGGVGFMDSSGIGLILGRYRLLQDTGGVLAVQGVSAQIAKMLRLSGIGSIITLE
                     GSANV"
     gene            1257..1688
                     /gene="spoIIAB"
                     /locus_tag="ACZPFK_04655"
     CDS             1257..1688
                     /gene="spoIIAB"
                     /locus_tag="ACZPFK_04655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR1405634.1"
                     /translation="MKPINQTKITFPSRSSNEAFARGAVAAFVAQLDPTVDELADIKT
                     AVSEAVTNCIVHAYPGAIGSVYITVSIFEDGRVRLRIADKGVGIEDVQAAMQPMSTTG
                     GEERAGLGFTVMQTFMDTLRVHSTPGKGTVVTMGKTIARRC"
     gene            1692..2363
                     /locus_tag="ACZPFK_04660"
     CDS             1692..2363
                     /locus_tag="ACZPFK_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1405635.1"
                     /translation="MPLKQCSRDEFIENNLGLVHACANRFRGRGIEYDDLYSAGCMGL
                     VKAYDAFDEERGVQFSTYAVPVILGEIKKLFRDGGSVKVSRSLKELSLKINAARDTLQ
                     KKTGAQPTVGELAAYLQVSPEQVAEAISAAMPVLSLTPVQDEDSPSQFDIPVNSDEEK
                     IAETLALRETLRRLDERDRLLIRCRFYEGKTQSETAKRLGTTQVQISRRERKLLAMLR
                     GLLTD"
     gene            2559..4079
                     /locus_tag="ACZPFK_04665"
     CDS             2559..4079
                     /locus_tag="ACZPFK_04665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405636.1"
                     /translation="MQKLTAFVRSSALYKVVALLVLLGAATLLSATLAINNVNIALLY
                     LLCVLLVCVVTGSNLYGMCAAICCAVLYNLLFLPPVGGFELTRADDLLTIAFYLVVSL
                     VAGALATQLQRRAWQAEESARRADLLARTGQGYLAMRGEEAVVRHGQSAIFELVGAEC
                     TVYLAGADGGLRPPLRFGAAGGEDENDPWVRRCFEEWRAGSPQPGPGAPGWENLPLAG
                     GERLLGVAAVRLGERRLNEADRSYLKAAVTQLSLSLEREKLSAEGERIQLEVEKEKLR
                     SSFLRSISHDLRTPLTGIAATVDYVARNAAVIDRDELGGLMRDVDRDAVWMNNMVTNL
                     LHLTRIQEGRLLVKKVPEVVDDVAADALERTRRLRCGRRVEAHLPDELLTVPMDGLLI
                     RQVLCNLLDNAFGHTRPDTAVRLTVSRRGQDALFAVEDDGGGIPPALLPHIFEGLVSE
                     ESATADGKRGMGLGLMVCAAIVQAHGGAIWAENNARGGAGFFFTLPLREEEESAHA"
     gene            4072..>4769
                     /locus_tag="ACZPFK_04670"
     CDS             4072..>4769
                     /locus_tag="ACZPFK_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1405637.1"
                     /translation="MREAPHILIVEDDPGIQRLLEMSLREIGYTCDLAATATGGYTLA
                     RANNPDLILLDLGLPDYDGVELLARIRQEQDTPVLIVSARGQEKDKIEALDAGADDYV
                     TKPFTLGEVQARIRANLRRTRPQIARSDVFEKDGLMLDFQRRRVSVDGKEIHLTPIEY
                     RLLVLLVQNPGKVLTHTYINREVWGYSDDESNQRLRVFMAGIRRKIEKDTANPRYILT
                     EVGVGYRFVDRAEE"
BASE COUNT          834 a         1484 c         1680 g          771 t
ORIGIN      
        1 gaaaaatgcg ggattctcaa gcccggctgt gtggcggtga gctacccggg acagcggccc
       61 ggggccgccg ccgtcatcgc ccggcgctgt gaggagctgg gcgtgccgct gcggctgccc
      121 gacccgcagg atatcaagct gctgtgggag accctgcgtg aaaatcatgt ggactacggc
      181 ggctatgagg tgcggctgcg gctgcacggc agccatcagg cggccaactg cgccctcgcc
      241 atagaggcgg ccctgggcct ctgcgaccag ggctacgaca tccccgacga ggcgatcctc
      301 tccgggctgg agcgggcctc cctgcccgcc agggccgagg tgctgtccct gcacccgccg
      361 gtattgctgg acggcgccca caacccggac agcgccgccg ctctggccgc cctgctgaaa
      421 accaccagga cgccgcccct caccgccgtg ctggggatgc tgaaaggcaa ggacgccgag
      481 ggggtcctga aagcgctgga ggggtgcttc gaccaggtgt acaccgtcgc gccggacacg
      541 ccgcgggcgg tgagcgccga tctgctggcc gcggccgcgt cggagcattt tcgccgcgtg
      601 gaggcctgcg gcagccttgc acaggcgctg gcaaaggccg aggcgcgggg cgggggcttt
      661 tgcgtctgcg gctcgttcta tctggcgggc gaggcgagac gtctgctcgg cgagggcgga
      721 aaactctcct ccggcctcga cagcctgtga cagaatgttt aaatgaaatc ccttatccta
      781 ggtgatggac aaaccatccc cgcggacgcg ccccggcggc agaccttgcc ggggacggtc
      841 cgggatcagg gatttttttg tttttagggc aactgcggcg ggcggacacg cccggcttgc
      901 ggtgagaaag ggaaaaagaa cagggaagtg aaaaatttgg cggaatgcag actggaacag
      961 agcggcccgg tgctggtggc gtatctctcg ggcgagatcg accaccacgc ggcggggcgg
     1021 ctgcgggaac agatcgacgc gcgcatcctc tcggcctccc cggagcgggt ggtgctggat
     1081 ttcggcggcg tgggctttat ggactcctcc ggcatcgggc tgattttggg ccggtaccgg
     1141 ctcttgcagg acaccggcgg cgtgctggcg gttcaggggg tcagcgcaca gatcgcaaaa
     1201 atgctcaggc tgtccggcat cggcagcatc atcacgctgg aagggagcgc aaacgtatga
     1261 agcccatcaa tcagaccaag atcacctttc ccagccgcag cagcaacgag gcgttcgcgc
     1321 gcggggcggt ggcggccttt gtggcccagc tcgaccccac tgtggacgag ctggcggata
     1381 tcaagacggc ggtgagcgag gcggtcacca actgcatcgt ccacgcctac cccggcgcca
     1441 tcggcagcgt gtacataacc gtgagcatct ttgaggacgg ccgggtgcgc ctgcgcatcg
     1501 cggacaaggg cgtcggcatc gaggacgtgc aggcagccat gcagcccatg tccaccaccg
     1561 gcggcgaaga gcgggccggc ctcgggttca cggtgatgca aacctttatg gacacgctgc
     1621 gggttcactc cacgcccggc aagggcaccg tcgtgaccat ggggaaaacc attgccagaa
     1681 ggtgttagcc tatgccgctc aaacagtgca gccgcgacga attcatcgag aacaacctgg
     1741 ggctggtgca cgcctgcgcc aaccgcttcc gcggccgcgg gatcgagtac gacgacctgt
     1801 attccgccgg atgcatgggc ctggtcaagg cttatgacgc gttcgacgag gagagggggg
     1861 tacaattttc cacctatgcg gtgccggtca tcctgggcga gatcaaaaag ctttttcggg
     1921 acggcggctc ggtcaaggtg agtcgctcgc tcaaggagct ctcgctgaag atcaacgccg
     1981 cgcgggacac gctgcagaaa aaaacgggcg cccagcccac ggtgggcgag ctggcggcct
     2041 atctgcaggt cagccccgag caggtggcgg aggcgatcag cgccgccatg ccggtgctct
     2101 cgctgacccc ggtgcaggat gaggacagcc cctcccagtt tgacatcccg gtgaacagcg
     2161 acgaggagaa gatcgccgag accctggcgc tgcgggagac gctgcgccgc ctggacgagc
     2221 gggatcggct gctgatccgc tgtcgctttt acgagggcaa gacccagagc gagacggcaa
     2281 agcggctggg caccacccag gtgcagatct ccaggcggga gcgcaagctg ctggcaatgc
     2341 tgcggggctt gctgaccgat taagcgggca gaggccgcaa aaataaggat ggaacagagg
     2401 tccggggcgg gaagcggcgc acaaggcgtc tcccgccccg gacctctgtt tgccggggcg
     2461 cggatattgg gatttttgcg cggatatggt atgataacag ggaaatacag atcatttccc
     2521 gcctcaagcg gcgggggagg gaaagggggc cgcccgccat gcaaaagctc acggcgttcg
     2581 tccgctcttc tgcgctctac aaggtggtcg ccttgctggt gctgctcggg gccgccacgc
     2641 tgctctcggc aacgctcgcc atcaacaatg tgaacatcgc cctgctctat ctgctctgcg
     2701 tgctgctggt ctgtgtcgtc accggcagca atctgtacgg catgtgcgcc gccatctgct
     2761 gtgcggtgct ctacaacctg ctctttctgc ccccagtggg cggctttgag ctcacccgcg
     2821 ccgacgacct gctcaccatc gccttttatc tggtggtcag cctggtggcc ggcgcgctcg
     2881 ccacccagct gcagcgccgg gcctggcagg cggaggagag cgcccgccgg gccgacctgc
     2941 tcgcccgcac cgggcagggc tatctggcca tgcgcggaga ggaggcggtg gtgcgccacg
     3001 ggcagtcggc catttttgag ctggtggggg ccgagtgcac ggtctatctg gccggtgccg
     3061 acggaggcct gcgaccgccg ctgcgctttg gcgccgccgg cggcgaggac gaaaacgacc
     3121 cctgggtgcg ccgctgtttt gaggagtggc gcgcgggcag cccgcagccc ggccccggcg
     3181 ccccggggtg ggagaacctg cccctggcgg gcggggaacg tctgctgggc gtcgcggccg
     3241 tccgtctggg cgagcgccgc ctcaacgagg ccgaccgctc ctatctcaag gcagcggtca
     3301 cccagctctc cctctcgctg gagcgggaaa agctctcggc agagggggag cgcatccagc
     3361 tggaggtgga aaaagaaaag ctgcgcagca gttttttgcg cagcatttcc cacgacctgc
     3421 gcaccccgct cactggcatc gccgccacag tggattatgt ggcccgcaat gccgccgtca
     3481 ttgaccggga cgagctgggc gggctgatgc gggacgtgga ccgggatgcg gtgtggatga
     3541 acaacatggt caccaacctg ctgcatctca cccgcatcca agaggggcgc ctgctggtca
     3601 aaaaggtgcc ggaggtggtg gacgacgtgg ccgccgacgc gctggagcgc acccgcaggc
     3661 tgcgctgcgg ccggcgggtg gaggcgcatc tgcccgacga gctgctcacc gtgccgatgg
     3721 acggcttgct gatccggcag gtgctctgca acctgctgga caacgctttc ggccacaccc
     3781 ggccggacac cgccgtgcgg ctcaccgttt cacggcgggg gcaggacgcc ctttttgcgg
     3841 tggaggacga cggcgggggc atcccgcccg cgctgctgcc ccacatcttt gaggggcttg
     3901 tgtcggagga gtccgcaacc gcggacggca agcggggcat gggcctgggg ttgatggtct
     3961 gcgccgccat tgtgcaggct cacgggggcg cgatctgggc ggaaaacaat gcgcgggggg
     4021 gcgcagggtt cttttttacg ctgcccctga gagaagagga ggagagtgcc catgcgtgag
     4081 gcgccgcata tcctgatcgt ggaggacgac cccggcatcc agcgcctgct ggaaatgtcg
     4141 ctgcgggaga ttggctatac ctgcgatctc gcagctaccg ccaccggggg ctacacactt
     4201 gcgcgggcca acaaccccga cctgattttg ctggatttgg gcctgccgga ttacgacggg
     4261 gtagagctgc tggcccgcat ccggcaggag caggacaccc ccgttttgat cgtctccgcc
     4321 cgcgggcagg aaaaggacaa gatcgaggcg ctggacgccg gggcggacga ctatgtcacc
     4381 aagcccttta cattgggcga ggtgcaggcg cgcatccgcg ccaacctgcg ccgcacccgg
     4441 ccgcagatcg cccgcagcga cgtgtttgag aaagacggcc tgatgctgga tttccagcgg
     4501 cgcagggtgt cggtggacgg aaaagagatc catctcaccc ccattgagta ccgcctgctg
     4561 gtgctgctgg tgcagaaccc aggcaaggtg ctcactcata cctatatcaa ccgcgaggtg
     4621 tgggggtaca gcgacgacga gagcaaccag cggctgcggg tctttatggc gggcatccgg
     4681 cgcaagatcg aaaaagacac cgcgaacccg cgctatatcc tcaccgaggt gggggtgggc
     4741 taccggtttg tggacagggc ggaggagga
//
LOCUS       DBMDYA010000139         4765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4329_length_4765_cov_1.662736, whole genome shotgun sequence.
ACCESSION   DBMDYA010000139 DBMDYA010000000
VERSION     DBMDYA010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_4765_cov_1.662736"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="ACZPFK_04675"
     CDS             <1..1002
                     /locus_tag="ACZPFK_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR1405638.1"
                     /translation="RDDTCTAWFAPRGGALFMDAAQSRLFCVTFASADQEVGDVLWEL
                     SLDGATRTKLYQFAASESFVDAVAADPEHLYFTARGPGEDPTSARKKLYALDLKSRAA
                     RLLTEYGDADWLFGAFDGTLVLLSFENEAGDESFTYRLYDLATGREKSFYHYGYLPDG
                     RRAVTSTDGPFLYVIEPSGEERARVLKLDMRDGGETLLCGGLPFYGGDLCQIQGFYDE
                     KMVVDVTRAEEETGRIEHRRYSVDCASGQIGELTLTVQDGLLEVFMPLLGGAADTQFL
                     VIERYLSREVTLTGNDGAPYASQRTWPVYALMDKADYWANKPDYRSIRDTGFYQEDE"
     gene            1004..1813
                     /locus_tag="ACZPFK_04680"
     CDS             1004..1813
                     /locus_tag="ACZPFK_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405639.1"
                     /translation="MKQDAPQTAQNTPQPPLLEVRDLSKSYPVKTGRGQNRAERRVHA
                     LRKVSFRLAPGLYGLLGPNGAGKSTLMNIITGNLDQDGGEVFWGGKNVHHLKARFLRV
                     LGYMPQQQGLYPGFSGRRFLEYFAALKEVPKAQIAAEAERAAALVNLSGELDKRLSAY
                     SGGMKQRLLAAQALIGDPRLLILDEPTAGLDPKERVRLRELMAGMAKERIVLIATHVV
                     PDVESVADGILLLRRGELADLGSPRELIEKYAPGRSLEDVYLTVFGDEDTP"
     gene            1810..4167
                     /locus_tag="ACZPFK_04685"
     CDS             1810..4167
                     /locus_tag="ACZPFK_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405640.1"
                     /translation="MKLFAAELHKLLKNRFCLGCLGALVLVNLFLLWTGVRPLAGRAP
                     ASAYRQMAQDLHGLPAPRQLALVEDRLRQTTALYRIDQIVRTEAMDPKGGALLRQQNA
                     DLFSEYGELYAAGGFLSYAPTLAQEYLFLTEIQQECAQVAGYDGFLDEIEEKAALLSQ
                     ISIFAASGGNDYAQQNIHRTAAAYEGMRGRAIDYAPQKGLMTALDFVWTDVVALFAML
                     AFASALVRDERDGGMLALVRSTPGGRLRTALAKLAALAAGMLGILLLLYGVNLLYCGS
                     LYGLGGLGRSIQSVPALMRSTLKVNVAEYLALFLLSKWAAAFLCGAWVLWTCLVAKRP
                     LTGYGAALLLPALSLLVRALVPATSRWNVLKYANLASLLKNNELLGGYRNLYWFGGPV
                     QLPLVELVAALLFGGCFISLFCFAFARAQLLPARRRATVRRTHKKASRARGLLPQEGY
                     KAFILCGAAAVLLGLGGFQMYQAAVSENFINAQEIYYARYMRRLAGPVTMEKLEWLQM
                     EGQKFRPLLLAQQGLAQGTVSPQQYQDIMMANYGLQQEYGVYQQVVGKLYYIKAHPGA
                     YFIYDSGYPKLFDHADTRDVQDVLLTGLALSMACCGLFAMEHATGMRRVVRATPLGRT
                     ATLAQKVGIGSGISAAVTLLVLAPRVWQVGCGYGFSAPTAPLYSLTEYADAPASIPVF
                     MLMFASLLARLIAGWSMAMTVLALSQRMKNQLYAALTGSVLLCLPSLLSVYGLTQAKW
                     FSFYPLFHFGAMMQRTDTAVAAWLCLALFLCLGLFCLYDLWDQWE"
     gene            complement(4431..>4765)
                     /locus_tag="ACZPFK_04690"
     CDS             complement(4431..>4765)
                     /locus_tag="ACZPFK_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1405641.1"
                     /translation="LCNHCGRCVSKCPFGAVEESTYGYKVYIGGRWGKKVAEGRPLSR
                     IFTSEDEVVALVEKAILFFRDEGVSGERFADTIARLGFDYVEDKLLNSVLDKESILKK
                     DVVGGATC"
BASE COUNT          762 a         1530 c         1557 g          916 t
ORIGIN      
        1 cgggacgata cctgcacggc ctggtttgcc ccccgcggcg gggcgctgtt tatggacgct
       61 gcgcagagca ggcttttttg cgtcaccttc gcctctgctg atcaggaggt gggggatgtc
      121 ctgtgggagc tctccctgga cggcgccacc cgcacaaagc tgtatcagtt tgccgcctct
      181 gagagttttg tcgacgccgt ggcggccgac ccggagcatc tgtacttcac cgcccgcggc
      241 ccgggcgagg acccgacgag cgcccgaaag aagttatacg cgctggacct gaaaagccga
      301 gcggcccgcc tgttgacgga gtatggggac gcggactggc ttttcggcgc gtttgacggt
      361 acgttggtgc tcttatcctt tgaaaacgag gcgggggacg agagctttac ctaccgcctt
      421 tacgacctgg ccaccgggcg ggaaaaaagc ttttaccact acggctatct gcctgacggc
      481 cgccgggcgg tcacctctac cgacggcccc tttctctatg tcatcgagcc ctccggcgag
      541 gagcgtgccc gggtactcaa gctggatatg cgggacggcg gtgagaccct gttgtgcggc
      601 ggcctgccgt tttacggcgg cgatctctgc cagattcagg gcttttacga cgaaaaaatg
      661 gtcgtggacg tcacccgggc ggaggaggag acggggcgga tcgagcaccg ccgctacagt
      721 gtggactgcg cgtccggcca gatcggcgag ctgaccctga cggtgcagga cggcttgctg
      781 gaggtgttca tgccgctgct gggcggggcc gcagatactc aattcctcgt catcgagcgc
      841 tatctctcca gagaggtgac cctcaccggc aacgacgggg ccccctatgc cagccagagg
      901 acatggccgg tctacgctct tatggacaag gccgactact gggccaacaa gcccgactat
      961 cgttccatcc gcgacaccgg tttttatcag gaagatgaat gacatgaaac aggacgcacc
     1021 gcagaccgca caaaacaccc cgcagccgcc gctgctcgag gtccgggacc tgagcaaaag
     1081 ctatcccgtc aaaaccgggc gcgggcaaaa cagggctgag cgccgcgtac acgcgctgcg
     1141 caaggtctcc tttcgccttg cgccggggct ctacggcctt ttggggccca acggggcggg
     1201 caaatccacc ctgatgaaca tcatcaccgg caacctggac caggacggcg gcgaggtgtt
     1261 ttggggcggc aagaacgtgc atcatttgaa agcgcgcttt ctgcgggtgc tggggtatat
     1321 gccgcagcag caggggctgt accccggctt tagcgggcgc cgctttcttg aatattttgc
     1381 ggccctcaag gaggtcccca aagcgcaaat cgccgccgag gccgagcggg cggccgcgct
     1441 ggtgaatctg tccggcgagc tggacaagcg gctgtccgct tactcgggcg gcatgaagca
     1501 gcggcttctg gccgcgcagg ccctgatcgg cgaccccagg ctgctgatcc tggacgagcc
     1561 aacggcgggg ctggacccca aagagcgggt gcgcctgcgg gagctgatgg ccgggatggc
     1621 caaagagcgg attgtgctca tcgccaccca tgtggtgccg gatgtggaga gcgtggccga
     1681 cggcattttg ctgctgcggc gcggcgagtt ggccgacctg ggcagcccgc gggagctgat
     1741 cgaaaaatat gccccgggcc ggtcgctgga ggacgtctat ctcaccgtgt tcggcgatga
     1801 ggatacgcca tgaagctttt tgcggcagag ctgcacaagc tcttgaaaaa ccggttctgc
     1861 ctgggctgcc tgggcgcgct ggtgctggtc aacctgtttt tgctctggac cggggtgcgg
     1921 cctttggccg gccgcgcgcc cgcctccgct taccgccaga tggcccagga cctgcacggc
     1981 ctgcccgcgc cccggcagct cgcgctggtg gaggaccggc tgcggcagac caccgctttg
     2041 tatcgcatcg accagatcgt gcgcaccgag gcgatggacc ccaagggcgg cgcgctgctg
     2101 cgccagcaga acgccgacct gttcagcgaa tacggcgagt tatacgctgc gggcggtttt
     2161 ttgtcctatg cccccacgct ggcccaggag tatctctttt tgactgagat ccagcaggag
     2221 tgcgctcagg tggcggggta cgacggcttt ctcgatgaga tcgaagaaaa ggcagcgctg
     2281 ctgtcgcaga tctccatctt tgcagccagc ggcgggaacg actacgccca gcagaacatc
     2341 caccgcaccg cggcggccta tgaggggatg cgcgggcggg caatcgacta tgcaccccaa
     2401 aagggcctga tgacggcgct ggattttgtc tggaccgatg tggtggccct gttcgccatg
     2461 ctggcctttg cctccgccct ggtgcgggac gagcgggacg gcggcatgct ggcgctggtg
     2521 cgcagcaccc ccggcgggcg gctgcgcacc gctttggcaa agctggcggc gctggctgcg
     2581 gggatgctgg gcattttgct gctgctctac ggcgtcaatc tgctgtactg cggctccctc
     2641 tacggcttgg gcggcctggg gcgcagcatc cagtcggtgc ccgccctgat gcgcagcacc
     2701 ctgaaagtga acgtggcgga atatctggcg ctgtttttgc tctccaaatg ggccgcggct
     2761 tttttgtgcg gggcctgggt tctttggacc tgcctggtgg cgaagcgccc cctcaccggt
     2821 tacggcgccg cgctgctgct gcccgccctg agcctgctgg tgcgcgcgct cgtgcccgcc
     2881 accagccgct ggaacgtgct caaatacgcc aatcttgcca gcctgctcaa aaacaacgag
     2941 ctgctgggcg gctaccgcaa tctttactgg ttcggcggcc cggtgcagct gccgttggtg
     3001 gagctggtcg ccgcgctgct gttcggcggc tgctttatct cgctgttctg ctttgccttt
     3061 gcccgggcac agctgctgcc ggcgcggcgc agggccacgg tgcgccgcac ccataaaaag
     3121 gccagccgtg cccgcggcct gctgccgcag gaggggtaca aggcctttat tctctgcggc
     3181 gcggcggcgg tgctgctggg gctgggcggc tttcagatgt atcaggctgc ggtgagtgaa
     3241 aatttcatca acgcgcagga gatctattac gcccgctata tgcgccgcct ggccggcccg
     3301 gtcaccatgg aaaagctgga atggctgcag atggaggggc aaaagttccg tccgctgctg
     3361 ctggcgcagc aagggcttgc ccagggcacg gtcagccccc agcagtatca ggacatcatg
     3421 atggcaaact acggcctgca gcaggaatac ggcgtttacc agcaggtggt gggcaagctc
     3481 tattacatca aagcgcaccc gggcgcctat tttatttacg acagcggcta tcccaagctg
     3541 ttcgaccacg cggatacccg ggacgtgcag gatgtgctgc tcaccgggct ggccctgtcg
     3601 atggcctgct gcggcctgtt cgccatggag cacgccaccg gcatgcggcg ggtggtgcgg
     3661 gccacgccgc tgggcaggac cgccaccctg gcccaaaagg tgggcatcgg cagcggcatc
     3721 tccgccgccg tcaccctgct ggtgctggcg ccgcgtgtct ggcaggtggg ctgcggctac
     3781 ggcttttccg cccccaccgc ccctctctac agcctgacgg aatacgcgga cgcgcccgcc
     3841 tccatcccgg tctttatgct gatgttcgcc tcgctgctgg ctcgcctgat tgcgggctgg
     3901 tcgatggcga tgacggtgct cgccctctcc cagcggatga agaaccagct ctacgccgcc
     3961 ctcaccggct ccgtgctgct ctgtctgccg tctctcttga gtgtgtacgg gttgacgcag
     4021 gcgaaatggt tcagctttta tccgctgttc catttcgggg cgatgatgca gcggaccgat
     4081 accgccgtcg cggcctggct gtgtctggcg ctgttcctct gtctggggct tttctgcctt
     4141 tatgacctct gggatcaatg ggagtgactc cgctcctcgc cggcgcctgc cggcggcgga
     4201 ggtttccgac tatcggccgt cagccgccgt ttctccactc cgtcaacaaa aaagacctcc
     4261 ccgggcgcac cgcccaggga ggtcttttcc attatccgcc gtctgtactc tatttctttg
     4321 agctttccct tttgcccacc gtgggcgccg cgccggacgg gcggcgggcc caaagctcgc
     4381 ttgagacccg ccgcccgggc attcgcccaa aggcgcgggc gaatgcgcgc tcagcaggtg
     4441 gcgccgccga ccacgtcctt tttcaggatg ctctccttgt ccagcacact gttgagcagc
     4501 ttatcctcca catagtcgaa gcccaggcgc gcgatggtat cggcaaagcg ctcgcccgag
     4561 acgccctcgt cgcggaaaaa caggatcgcc ttttccacca gggccaccac ctcgtcctca
     4621 ctggtgaaga tgcgggacag cgggcggccc tccgccacct ttttgcccca gcggccgccg
     4681 atgtacacct tgtagccgta ggtgctctcc tccaccgcgc cgaacgggca cttggacacg
     4741 cagcggccgc agtggttgca gagct
//
LOCUS       DBMDYA010000140         4757 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4340_length_4757_cov_1.512027, whole genome shotgun sequence.
ACCESSION   DBMDYA010000140 DBMDYA010000000
VERSION     DBMDYA010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_4757_cov_1.512027"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="ACZPFK_04695"
     CDS             <1..795
                     /locus_tag="ACZPFK_04695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIR1405642.1"
                     /translation="VSEIIHRGGTMLYTARCLEFKTLEGQKKAADRCRELGIDALVVI
                     GGDGSFRGCKDMAAQGIPCVGLPGTIDNDIACSDYTIGYDTAMNTAVEMIDKLRDTTQ
                     SHDRCSVVEVMGRNAGYLALNTGIAVGALATLIPEMPFDIEKDIVQRMNDTRRTGKQH
                     FIIIVAEGVGRAHDFAEIIEKKTGIESRATVLGHVQRGGSPTLRDRVSASQMGYHAVQ
                     LLLDGKFNRVVGTRGNDIVDFDITEALNMHKSIDTSLYEMSKVISI"
     gene            complement(925..1551)
                     /locus_tag="ACZPFK_04700"
     CDS             complement(925..1551)
                     /locus_tag="ACZPFK_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405643.1"
                     /translation="MAFGNFMNMEKLSLTIAENHIARMTDGTYDAHDCSLDMERLTGN
                     IENGFVAITQLSCYFESFLNTIVSTCMHYDGEALLKCSVEEKLDLIFMHYQRDWKSLK
                     GQAPWEIYRKATRVRNEMIHYKKTYIGEGTGIPDFKLGGQGAAEFFTQKSMKAVYDGH
                     IKLAESIAAMLGLRIFAEIGIFACDGRDGLVNYVYDPGETAVDQSRFE"
     gene            complement(1768..3135)
                     /locus_tag="ACZPFK_04705"
     CDS             complement(1768..3135)
                     /locus_tag="ACZPFK_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405644.1"
                     /translation="MRKSLILTGVLLLLSVGLLVGASSLIGSRETAVFIETATLYGDP
                     SAAEGIAVQLHAGCKGHLFWDILCRAGEKPHATTAFTFSGSERQETQREAPSVSLAFS
                     CDGGISGSDISFEDDDEWGMMTRPAADLAKRTPVGETRTETVSLADYYDTYPLFFDLQ
                     LPGQAYNGIEGLQKAVRAYFQLPVPASHRVKVTVQKDGDGNTVNVELSSVGEPLSLST
                     VSVVTENGCYFAFSGHRKGRDLLDLSHIPGGYGLYYLPFEKEQQEGGLVTLKAGEIGM
                     VFPLDESQSEVCALAAGAGGKELFLVTREQDAFWLTVLDTAEMRPSQKFPVLPAGEEL
                     SFWQQWVYPDFVALQVSDGGLALLHRGENGVFETAFTCGGGEQWTERVRYDSIFAYDG
                     ARLAVATPMDRSLCSFYLSVYEKNGLAYAGQYQNSLDLSPTTDYAYRCRLVGPDPLEL
                     GFLPA"
     gene            complement(3139..3873)
                     /locus_tag="ACZPFK_04710"
     CDS             complement(3139..3873)
                     /locus_tag="ACZPFK_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405645.1"
                     /translation="MLKLESVSKSYGRKQALDRVDLELRPGELVGLFGENGAGKTTLM
                     KCILGYLRHGGAITLDGAPVTRKNISRLSFATSEHSFFPTLSAEAHREFYQLHFPLFR
                     EKRFEGLMDFFELPRNRELRRFSTGQQNQFEVILALCQGADYILLDEPFAGNDVFNRE
                     DFYKVLLGILEPGETVLLSTHLLEEVETFISRAVLLRRGRVVGDVSMMALEEAGRSLM
                     SFVKETYHYRPDRVSRALSDLAPEEE"
     gene            complement(3889..4365)
                     /locus_tag="ACZPFK_04715"
     CDS             complement(3889..4365)
                     /locus_tag="ACZPFK_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405646.1"
                     /translation="MKSKLSALVPLGVSASAEIKWIAAGLSLSSLYSLKFVFNFLSRR
                     QDLLWMIENKLDGVMPAFFTLLDHGFAGFPAVAACMAGLALYHWFYHYQGSKSIYLMR
                     RLPRRWELLRRCAALPALGAAACLCAALLLLLLYCLIYLFFTPRGYLPAQPWHYYF"
     gene            complement(4349..>4757)
                     /locus_tag="ACZPFK_04720"
     CDS             complement(4349..>4757)
                     /locus_tag="ACZPFK_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405647.1"
                     /translation="ICFFLFWAAQLLTALLLCRLYLSSSEPALRGQTVFLAFYRVPFL
                     HSLLPLRDLGRAARDLALLVGLAITTACFPLRQRHGERRLEPLALAAVTAVAFAQPMG
                     KFSSDLLLSLIALALGGSALFSLWKEVLDEEQA"
BASE COUNT          984 a         1436 c         1465 g          872 t
ORIGIN      
        1 gtctccgaga tcatccaccg cggcggcacc atgctctaca ccgcccgctg cctggagttc
       61 aagacccttg agggccaaaa gaaagcggcc gaccgctgcc gcgagctggg catcgacgcg
      121 ctggtcgtca tcggtggcga cggctctttc cgcggctgca aggacatggc ggcgcagggc
      181 atcccctgtg tgggcctgcc cggcaccatc gacaacgata tcgcctgcag cgattacacc
      241 atcggctatg acaccgccat gaataccgcc gtggagatga tcgacaagct gcgcgacacc
      301 acccagagcc atgaccgctg cagcgtggtg gaggtcatgg gccgcaatgc cggctatctg
      361 gcgctcaaca ccggcatcgc cgtgggcgcg ctggccaccc tgatccccga gatgccgttc
      421 gacatcgaga aggatatcgt gcagcgcatg aacgataccc gccgcaccgg caagcagcac
      481 ttcatcatca tcgtggccga gggcgtgggc cgggcgcacg attttgccga gatcatcgag
      541 aaaaagaccg gcatcgagag ccgcgctacc gtattgggcc atgtgcagcg gggcggcagc
      601 cccaccctgc gcgaccgcgt gtccgcgagc cagatgggct atcacgccgt gcagctgctg
      661 ctggacggca agttcaaccg cgtggtgggc acccgcggca acgacatcgt tgatttcgat
      721 atcaccgagg cgctgaacat gcacaagagc atcgacacct cgctgtacga gatgtccaag
      781 gtgatctcca tctgatgcct taaaacagca aaaaaacatc ctgcctgaaa aagtatcagg
      841 caggatgttt ttttgtcggc agggacggcg cggcaaacgc cgtctttctc ccgtccgcgc
      901 aagccgaaaa aagtccaggc cgttctactc aaatctggat tggtcgactg cggtctcgcc
      961 ggggtcatat acatagttga ccaggccgtc tcttccgtcg catgcaaaga tgccgatctc
     1021 cgcaaatatc ctgagcccca gcatggcggc aatcgattct gccaatttta tgtgtccgtc
     1081 gtacacggct ttcatgctct tttgggtgaa aaattccgct gcgccctgcc cgcctagctt
     1141 gaagtccggt atccctgttc cctcgccgat gtaggtcttt ttatagtgga tcatttcatt
     1201 tctcactctt gtcgcttttc tgtaaatctc ccacggggct tgacccttga ggcttttcca
     1261 gtccctttga taatgcataa agatcaagtc aagcttttct tccacgctgc acttcaacag
     1321 cgcttctccg tcatagtgca tacaggtgct gacgatggtg ttcagaaaac tctcaaaata
     1381 gcagctcagc tgggtgatgg cgacaaagcc gttttcgata ttgccggtca gcctttccat
     1441 atccaaagag cagtcgtggg catcataagt tccgtcggtc atccgggcga tatgattttc
     1501 ggcgatggtg agagataatt tttccatatt cataaaattg ccaaacgcca taatttttca
     1561 ctctccttgg gcgtatcact tatttcccgt gaaagcaggg gtgtgcgtgg agcgctgttt
     1621 gacggtttga cagggctcgg cgctcgtctt tttcaaaagg caaaggggcg ctgcgcgttg
     1681 tccgtcgccg cagggccgcc gtaccatgtt ttttgcattt gagccgggtc gatgctttct
     1741 ccggagtgga gctgcaggag cggccggtca ggcgggcaaa aagcccagct ccagcgggtc
     1801 cggtcccacc aggcggcagc ggtaggcata gtcggtggtg ggggagagat cgaggctgtt
     1861 ttggtattgc ccggcgtagg ccaagccgtt cttttcataa acggagagat aaaagctgca
     1921 cagggaacgg tccatcggcg tggcgaccgc cagacgcgca ccgtcatagg cgaagatgct
     1981 gtcgtagcgc acccgctcag tccattgctc cccgccgccg caggtgaatg ctgtctcaaa
     2041 gacaccgttc tcgcctctgt gcagcagcgc aaggccgccg tcgctgacct gcagcgccac
     2101 aaaatcagga tagacccact gctgccagaa agagagctct tcccccgcag gcaggaccgg
     2161 aaatttctgt gacgggcgca tctccgctgt atccagcacc gtcagccaaa aggcgtcctg
     2221 ctcgcgggtg acgaggaaca gctcctttcc ccccgcgccg gcggccaggg cgcagacctc
     2281 gctttggctc tcgtcgaggg ggaataccat gccgatctca ccggccttga gggtgacgag
     2341 gccgccctcc tgctgttctt tttcaaaggg gaggtaatag aggccgtagc cgcccggaat
     2401 atggctgagg tccagcaggt cgcggccctt gcggtggcct gaaaaggcaa agtagcagcc
     2461 gttttcggtc acgaccgaca cggtggacag ggagaggggc tcgcccacag aggacagctc
     2521 cacattgacg gtgttgccgt cgccgtcctt ttgcaccgtg actttcactc gatggcttgc
     2581 ggggacgggg agctgaaaat aggcccgcac cgccttttgc agcccttcga tgccgttata
     2641 agcttggccc ggcagctgga ggtcgaaaaa aagaggatag gtatcgtaat aatccgccag
     2701 ggataccgtc tcagtgcgcg tttcaccgac gggggtgcgc tttgccaggt cggcggcggg
     2761 gcgcgtcatc atcccccact catcgtcgtc ctcaaagctg atatctgagc cgctgatgcc
     2821 gccgtcgcag ctgaaagcga gagagaccga gggggcctcc cgctgcgttt cctgccgttc
     2881 cgagccggaa aaagtgaaag cggtggtcgc gtgcggcttc tcgccggcgc ggcagaggat
     2941 atcccaaaac aggtggcctt tgcacccggc gtgcagttgt acggctatcc cttcggccgc
     3001 gctggggtcg ccgtagaggg tggcggtctc gatgaaaaca gccgtctcac ggctgccgat
     3061 cagggaggag gctcccacca ggaggccgac ggagagcagc agcagaacac cggtcaggat
     3121 caggcttttg cgcatgggtc attcctcctc cggggcgaga tcggacaggg cgcggctgac
     3181 ccggtccggc cggtagtgat aggtctcctt gacaaaactc atcaggctgc ggcccgcttc
     3241 ttccagcgcc atcatagaca cgtcccccac caccctgccg cggcgcagca gcaccgcccg
     3301 gctgataaag gtctccacct cctccagcag atgggtggaa agcagcacgg tctcgcccgg
     3361 ctccaaaatg cccagcagca ccttgtaaaa gtcctcgcgg ttgaagacgt cgttcccggc
     3421 gaaaggctcg tccagcagga tgtaatccgc cccttggcag agcgccagaa tcacctcgaa
     3481 ctggttttgc tgcccggtgg aaaagcggcg cagctccctg ttgcgcggca gctcgaaaaa
     3541 gtccatcagc ccctcaaagc gcttttcgcg gaacagggga aagtgcagct gataaaactc
     3601 acggtgagcc tctgcggaca gggtggggaa gaaagagtgt tcgctggtgg caaaggagag
     3661 gcggctgatg tttttgcggg tcacaggcgc cccgtccaac gtgatggcgc ccccgtggcg
     3721 cagatagccc agaatgcatt tcatgagcgt ggtcttgccc gcgccgtttt ccccaaacag
     3781 gcccaccagc tcaccgggcc gcagctccag atccacccgg tcgagcgcct gtttgcgccc
     3841 gtaggatttg cttaccgatt ccagcttgag catcgtctcg gccccctttc aaaaatagta
     3901 gtgccacggc tgcgcgggca gatagccgcg cggcgtaaag aagagataga tcaggcagta
     3961 gagcagcaga agcagcagtg cggcgcaaag gcaggccgcg gcccccaggg cgggcagcgc
     4021 ggcgcagcgg cgcaagagct cccagcgccg gggcaggcgg cgcatcaggt agatgctctt
     4081 gctgccctgg tagtggtaga accagtgata aagggcgagg cccgccatac aggccgccac
     4141 ggcgggaaag cccgcgaagc cgtggtccag cagggtgaaa aaggcgggca tgacgccgtc
     4201 caacttgttc tcgatcatcc agagcagatc ctggcggcgg gagagaaaat tgaacacgaa
     4261 ttttaggctg taaagagagc tgagcgagag cccggcggcg atccatttga tctcggccga
     4321 ggcggagacg cccaggggaa ccagcgcgct aagcttgctc ttcatcgagg acctccttcc
     4381 acaggctgaa cagagccgag ccccccagcg ccagcgcgat cagggagagc agcaggtcgg
     4441 agctgaactt ccccatgggc tgggcaaagg cgacggcggt cacggcggcg agcgccaacg
     4501 gctccagccg ccgctcgccg tgccgctgcc gcagggggaa gcaggccgtc gtgatcgcca
     4561 gccccaccag aagcgccagg tcgcgggccg cccggcccag atcccgcagg ggcagcagac
     4621 tgtgcagaaa ggggacgcgg tagaaagcga gaaagacggt ctggccgcgc agggcgggct
     4681 cggaggacga cagataaagg cggcacagaa gcagcgcggt caaaagctgc gccgcccaaa
     4741 acagaaaaaa gcagatg
//
LOCUS       DBMDYA010000141         4727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4376_length_4727_cov_1.169909, whole genome shotgun sequence.
ACCESSION   DBMDYA010000141 DBMDYA010000000
VERSION     DBMDYA010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4376_length_4727_cov_1.169909"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="ACZPFK_04725"
     CDS             complement(<1..902)
                     /locus_tag="ACZPFK_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR1405648.1"
                     /translation="MAELSPMMKQYFEIKEQYKDTILFFRLGDFYEMFFDDAILVSKE
                     LELTLTGRDCGQDERAPMCGVPYHACETYLARLIAKGYKVAICEQMEDPAKAKGLVKR
                     DIIRVVTPGTVIESSMLDDAKNNYIGSIFLRGKEAGVCFADISTGVCHATQLTEGDLA
                     GGIIGELCRFAPSELLFNSEILKHKEITAYVKDHMSCSVELLDEADYEPKAAEERMRG
                     QFGPGYEQAAGVRKNSVAFYSLSALLAYLAGTQKRGIERLKTVEVYSGSQYMQLSPVT
                     RANLELTETLRSKEKKGSLLWVLD"
     gene            complement(1199..1603)
                     /locus_tag="ACZPFK_04730"
     CDS             complement(1199..1603)
                     /locus_tag="ACZPFK_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIR1405649.1"
                     /translation="MDIIDQVRALALELQKDERYLVLENARRMNDADEELQQQIGEFN
                     LARIDLNNEVSKPEKNAERITELNEKIQSIYTDVMNNESMQAYNEAKTEMDNLMQYIN
                     AILTTAVNGGDPMTVEEPSSCGGDCSGCSGCH"
     gene            complement(1768..3153)
                     /gene="miaB"
                     /locus_tag="ACZPFK_04735"
     CDS             complement(1768..3153)
                     /gene="miaB"
                     /locus_tag="ACZPFK_04735"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1405650.1"
                     /translation="MEYQDFTELDRVIDEIGNYYDHAPAAYVHSFGCQQNVNDGEKIK
                     GVLQQAGYSLTDRAEGADLVVFNTCAVREHAQQRVYGNIGALKPLKAANPKMLIAIGG
                     CMVQQREVVDKLKQSYPYVDVVFGVNAIDRLPGLLAQRIRAKKRVLMDPAERYDIIDN
                     LPIHRDSAFKAWLPIMYGCDNFCSYCIVPFVRGRERSRTPEDILAEFRTLVQSGYKEI
                     TLLGQNVNSYGKGLDKKIDFSDLLALLAEQEGDFRLRFMTSHPKDASRKLIDTIASHE
                     KICKNLHLPVQSGSDRILVQMNRKYDIAQYKGLIEYAKHTVPEMTFSSDVMVGFPGET
                     EEDFEQTVQLVKEVRYIQLFTFIFSKRTGTKAALMADDTPHAEKARRINRLIKLQEEI
                     AVEEAVALAGRSFKVLVEGPGRQPGQLSGRLDNNLVVEFDGDAALVGGFAQVKITGSK
                     GIYLQGGLEPQ"
     gene            complement(3240..>4727)
                     /gene="uvrA"
                     /locus_tag="ACZPFK_04740"
     CDS             complement(3240..>4727)
                     /gene="uvrA"
                     /locus_tag="ACZPFK_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1405651.1"
                     /translation="ELSEREHLIGDRILKEIRERLGFLQSVGLEYLTLARNAQTLSGG
                     ESQRIRLATQIGSYLVGVLYILDEPSIGLHQRDNDKLIATLKRLRDLGNTLIVVEHDE
                     DTMRAADYVVDIGPGAGVHGGELVACGTVEDIMAEPRSITGQYLSGKKKIKVPKERRK
                     GAGQVLRVVGANQNNLKDITVEFPLGVMTCVTGVSGSGKSSLVNDILYKKLAADLNGA
                     KLRPGSFQEIEGMEYIDKVIGIDQSPIGRTPRSNPATYTGVFTDIRNLYAQTTDAKMR
                     GYNSGRFSFNVKGGRCEACEGDGIIKIEMHFLPDIYVPCEVCKGKRYNRETLEVKYKD
                     KSIYDVLDMTAEEAVVFFENIPRIKRKIQTLVDVGLGYIKLGQSATTLSGGEAQRVKL
                     ALELARVSTGKTVYILDEPTTGLHTADVHKLIKVLQKLVDAGNTVIVIEHNLDIIKVS
                     DHIIDLGPEGGNAGGEIVATGTPEEVADNPRSYTGQYLKPMLARK"
BASE COUNT          768 a         1518 c         1426 g         1015 t
ORIGIN      
        1 ttgtccagca cccagagcag ggagcccttt ttctccttgc tccggagggt ctcggtgagc
       61 tccaggttgg cgcgggtgac gggcgagagc tgcatgtact ggctgccgga gtagacctcc
      121 accgttttca gccgctcgat gccgcgcttt tgggtgcccg ccagataggc cagcagcgcg
      181 gacaagctgt aaaaggccac gctgtttttg cgcacccccg ccgcctgctc atacccgggc
      241 ccaaactgcc cacgcatccg ctcctcggct gctttcggct catagtccgc ctcgtccagc
      301 agctccaccg aacagctcat gtggtccttg acataggcgg tgatctcttt gtgcttgagg
      361 atctcgctgt tgaacagcag ctcgctgggc gcaaaccggc acagctcgcc gatgatgccc
      421 ccggcaaggt ccccctcggt gagctgggtg gcgtggcaga cgccggtgga gatatccgca
      481 aagcagaccc ccgcctcctt gccgcgcagg aagatcgagc cgatatagtt gttttttgcg
      541 tcgtccagca tactgctctc gatgaccgtg cccggcgtca ccacgcggat gatgtcccgc
      601 ttgaccaggc ccttggcctt ggccgggtcc tccatctgct cgcagatagc gaccttgtag
      661 cccttggcga tcagccgcgc gagataggtc tcacaggcat gatagggcac gccgcacatg
      721 ggcgcccgct cgtcctgccc gcagtcccgg ccggtgaggg tcagctccag ctctttggac
      781 accagaatcg cgtcgtcaaa gaacatctcg taaaaatccc ccagccggaa aaacaggatc
      841 gtgtccttat actgctcttt aatctcaaaa tattgcttca tcatgggaga aagctccgcc
      901 aaaccgcgca ccacctgtca gaatgcaggc ggccgggcgc actgcgcacc gccgccgtgt
      961 tcgaaatttt ttaaaacttg aaatttgaaa aaagaagccg cggaagcgcc ccgtggaact
     1021 gccgcggaac gccccctgcg gcgtcgttct tgtactggat ccccgcgcgc gggggctgag
     1081 ccctctacgc tgaacaactg gcttaacatg gggcgccttg tactggatcc ccgcgcgcgg
     1141 gggctgagcc cctcttgttg cgggagtggg ccggcgcccg gggcgccctg ccgccgtttt
     1201 agtggcagcc gctgcagccg gagcaatcgc cgccgcagga gctgggctcc tccaccgtca
     1261 tcgggtcgcc gccgttcacc gcagtggtga ggatggcgtt gatatactgc atcaggttat
     1321 ccatctcggt cttggcctcg ttgtaggcct gcatgctctc gttgttcatg acgtcggtgt
     1381 agatgctctg gatcttttcg ttgagctcgg tgatgcgctc tgcattcttc tccggcttgc
     1441 tcacctcgtt gttgaggtcg atgcgcgcca ggttgaactc gccgatctgc tgctgcagct
     1501 cctcatcggc gtcgttcata cggcgggcgt tttccagcac cagatagcgc tcgtcctttt
     1561 gcagctccag cgcgagcgct ctgacctgat cgatgatgtc catatctgat ctccttttag
     1621 aataaaataa tttgattgag agtgaaaagc cgcgggcggg cgacggatcg cagcgccgcc
     1681 tttgggcggc cttgcccggc agggcccgcg ccaagcagcc ccaaattttc catcgccccg
     1741 cgcggaaaga cccgcgccgc ggcgcgctca ctgcggctcg aggccgccct gtaagtaaat
     1801 ccccttgctg ccggtgatct tcacctgggc gaagccgccc accagggctg cgtcgccgtc
     1861 gaattccacc accagattgt tgtcgagccg gccggacagc tggcccggct ggcgcccggg
     1921 tccctccacc agcaccttaa agctgcggcc cgccagggcg accgcctcct cgacggcgat
     1981 ctcctcctgc agcttgatga gccggttgat gcgccgcgcc ttttcagcgt gcggggtatc
     2041 gtccgccatc agcgctgcct tggtgccggt gcgctttgaa aaaataaagg tgaacagctg
     2101 gatatagcgc acctctttca ccagctgcac cgtctgctca aaatcctcct cggtctcgcc
     2161 gggaaagccc accatgacgt cgctggaaaa ggtcatctcc ggcaccgtgt gctttgcgta
     2221 ctcgatcagc cccttatact gggcgatgtc gtatttgcgg ttcatctgca ccagaatgcg
     2281 gtccgagccg ctctgcaccg gcagatgcag gtttttgcag attttttcgt gggaggcgat
     2341 ggtgtcgatc agcttgcggc tggcgtcctt gggatggctg gtcatgaacc gcagccggaa
     2401 atcgccctcc tgctccgcca gcagggccag cagatcggag aaatcgattt ttttatcgag
     2461 ccccttgccg tagctgttga cattctgccc cagcagggtg atctctttat agccgctctg
     2521 caccagggta cggaactcgg ccaggatatc ctccggggtg cggctgcgct ctcgcccgcg
     2581 cacaaagggc acgatacagt aggagcaaaa gttgtcgcag ccgtacatga tgggcagcca
     2641 ggccttgaac gcgctgtccc tgtggatggg caggttgtcg atgatatcat agcgctcggc
     2701 cgggtccatg agcacccgct ttttggcacg gatgcgctgg gccagcaggc cgggcagccg
     2761 gtcgatggcg ttcaccccga acaccacgtc cacatagggg tagctctgtt tgagcttgtc
     2821 caccacctcg cgctgctgca ccatacagcc gccgatggcg atgagcatct tggggttcgc
     2881 cgctttgagg ggcttgagcg cgccgatatt gccgtagacc cgctgctggg cgtgctcgcg
     2941 cacggcacag gtgttgaaga cgacgaggtc ggccccctcg gcccggtcgg tcaggctata
     3001 gcccgcctgc tgcagcacgc ccttgatctt ctcgccgtca ttgacgttct gttggcagcc
     3061 gaacgagtgg acataagccg cgggagcgtg gtcgtagtag ttgccgatct cgtcgatcac
     3121 gcggtcgagc tctgtaaaat cctgatactc caaagcttca cctccatcgt tttattatac
     3181 acctttttgc ggttttgcac aacttacccc tcccgcccgc cgccgcggcg caaggcggct
     3241 tattttctgg ccagcatcgg cttgaggtat tgcccggtgt agctgcgggg gttgtccgcc
     3301 acctcttcgg gggtgccggt ggcgacgatc tcgccgcccg cgttgccgcc ctcgggcccc
     3361 aggtcgatga tgtggtcact gaccttgatg atgtccagat tgtgttcgat gacgatcacc
     3421 gtattgcccg cgtccaccag cttttgcagc accttgatga gcttgtgcac gtcggcggta
     3481 tgcaggccgg tggtgggctc gtcgaggata tacaccgttt tgccggtgct cacccgcgcc
     3541 agctccagcg ccagcttgac ccgctgcgcc tcgccgccgg acagggtggt ggcgctttgg
     3601 cccagcttga tatagcccag gcccacgtcc accagcgtct ggatcttgcg cttgatgcgc
     3661 gggatgttct caaagaagac taccgcctcc tccgccgtca tgtccagcac gtcgtagatg
     3721 ctcttgtcct tgtacttgac ctccagcgtc tcccggttgt agcgcttgcc cttgcagacc
     3781 tcgcagggca catagatatc cggcagaaag tgcatctcga tcttgatgat gccgtcgccc
     3841 tcacaggcct cgcagcggcc tcccttgacg ttgaacgaga agcggccgga gttatagccc
     3901 cgcatcttgg cgtcggtcgt ctgggcgtag aggttgcgga tatcggtgaa cacgccggta
     3961 taggtggccg ggttcgagcg cggggtgcgg ccgatgggcg actggtcgat gccgatgacc
     4021 ttgtcgatat actccatgcc ctcgatctct tgaaagctgc ccgggcgcag cttggcgccg
     4081 ttgaggtccg cggccagctt tttgtagagg atgtcgttga ccaggctgga cttgccgctg
     4141 cccgagacgc cggtgacgca ggtcatgacc ccgaggggaa actccaccgt gatgtccttg
     4201 agattgttct ggttcgcccc caccacgcgc agcacctggc cggcgccctt gcggcgctct
     4261 ttgggcacct tgatcttttt tttgcccgac aggtactgcc cggtgatgga gcgcggctcg
     4321 gccatgatgt cctccaccgt gccgcaggcc accagctcgc cgccatgcac gcccgccccg
     4381 gggccgatat ccaccacata gtccgcggcg cgcatggtgt cctcgtcgtg ctccaccacg
     4441 atcagggtgt tgcccaggtc acgcagccgt ttgagggtgg cgatgagctt gtcgttgtcg
     4501 cgctggtgca ggccgatgct gggctcgtcc aggatataca gcacccccac cagataagag
     4561 ccgatctggg tcgccaggcg gatgcgctgg ctctcgccgc cggacagggt ctgggcgttg
     4621 cgggcgaggg tcagatactc caggcccacg ctctgcaaaa agcccagccg ctcgcggatc
     4681 tccttgagga tgcggtcgcc gatcaggtgc tcgcgctcgc tgagctc
//
LOCUS       DBMDYA010000142         4688 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4419_length_4688_cov_1.427731, whole genome shotgun sequence.
ACCESSION   DBMDYA010000142 DBMDYA010000000
VERSION     DBMDYA010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4419_length_4688_cov_1.427731"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(525..950)
                     /locus_tag="ACZPFK_04745"
     CDS             complement(525..950)
                     /locus_tag="ACZPFK_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405652.1"
                     /translation="MKKDRFGICLTFYAVLAFVLAILGQTLLGGLLLAFAILVEQDEW
                     LIRQTMQAFFLSLVSGVVTAFINAVSWLYAVPILGAVVSGFFGIVTGIISLVILVFAI
                     VGLVNVAKGKDAGVPLCKGLADKAFGLVKTVSYTQGPEQ"
     gene            complement(1167..2942)
                     /gene="argS"
                     /locus_tag="ACZPFK_04750"
                     /pseudo
     CDS             complement(1167..2942)
                     /gene="argS"
                     /locus_tag="ACZPFK_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
     gene            complement(2939..3361)
                     /locus_tag="ACZPFK_04755"
     CDS             complement(2939..3361)
                     /locus_tag="ACZPFK_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1405653.1"
                     /translation="MKEDYLIRIKGIQESEGEENAVELMTRGSFVQRGGSYYITYRET
                     EATGYDGCTTTVKLDKAGTVSMLRFGPAPSQLVIEKGRRHICHYDTGHGAITMGIAAD
                     EIVADLSERGGSLRFSYNLDINSSLFSKNTVNITVREA"
     gene            complement(3430..4242)
                     /gene="murI"
                     /locus_tag="ACZPFK_04760"
     CDS             complement(3430..4242)
                     /gene="murI"
                     /locus_tag="ACZPFK_04760"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1405654.1"
                     /translation="MDNRPIGVFDSGLGGLTGVKELVRLLPGEDIVYFGDTARVPYGG
                     RSADTIIRYGKEDVQFLLSKGVKAVLCACGTVSSTLPAAVTEALPVPFVGVVQAAAAA
                     AAEASGSGRIGVIATAASIRSGGYEAAVRALRPDSALTARACPLFVPVIENGYVGRRD
                     PIVTALAKEYLAPVRQAGVDTLILGCTHYPLIAGAIADEMGPGVRLIDSGAEGAAALR
                     RLLERQGLLNGRAEGGAVSFYVSDSGENFAEVGGLFLGKDVHGTVHPFTLWD"
     gene            complement(4235..>4688)
                     /locus_tag="ACZPFK_04765"
     CDS             complement(4235..>4688)
                     /locus_tag="ACZPFK_04765"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR1405655.1"
                     /translation="LALAHDTRIVFERFVTGQEVECAVMGNDGPQGTLPGEILASQEF
                     YTYDDKYIAGTSRVVIPAQLSPEKLEEVRATAVRAYRTLCCTGLARVDFFVEAGTGRV
                     LLNEINTMPGFTDISMYPKLMLHAGMSYGGLLDRLIQLAFERKETPNG"
BASE COUNT          753 a         1582 c         1574 g          779 t
ORIGIN      
        1 gcggcccgcg ccttttcttt cccccattat accccatgcc gccgcaaaga aaaagcagcc
       61 tcccaaaagg tgcgtctccg cgggcgcgca ttgcccgcgg gcggggcggg cctgcgcgcc
      121 gagcgccgtc aaatgcgccc cgcagccgcg gcggggctcg aagattcgcc ccgggatcag
      181 cacgtctccg gttgccgcgt cggccgcggc cacagggggg cttgggggtc accgtgcggg
      241 ctgactgcac ggacgggcga cccgcgtggg gttttccgac gcggcatcgc gatttccctc
      301 ccgggggcgc ggcagctttc aaccggcggc accgggaaaa gcacagtgca aaaaaggcct
      361 gcaatccaca gggattgcag gcctttttta gtatacgggg cgttaagacg gtcggtccaa
      421 aatcaatgcg ttcgcccctt tgcgccccga ggcggagact ggacccgctc tctgcgggcg
      481 ggagggcggg cgcgcgcccg gcaaaagacg gcggccgcgg cctgttattg ctccggcccc
      541 tgggtatagc tgacggtctt caccaggccg aacgccttgt cggcaaggcc cttgcagagg
      601 ggcacgcccg cgtccttgcc cttggccacg ttgaccaggc cgacgatggc aaagaccagg
      661 atgaccaggg agatgatgcc ggtcacgata ccgaagaatc ccgagaccac ggcgcccagt
      721 atggggacgg cgtagagcca ggagacggcg ttgatgaaag cggtgacgac gcccgagacc
      781 agggacagga aaaacgcctg catggtctgg cggatcagcc actcgtcctg ctccaccagg
      841 atggcgaaag cgagcagcag cccgcccagc agggtctggc ccaggatggc cagcacgaaa
      901 gccagcaccg cgtaaaaggt gaggcagatg ccgaaacgat cttttttcat gatgattcct
      961 ccttatttac aggtcacggg gctccgcctc acacggaccc ccacttcatt atacaggaaa
     1021 agagagcaaa ggaacaggga gacaggggaa aaaacgcttt gcgaagacac cttttgcgcc
     1081 cgggcgcacc ccggcgcgga aaaggataaa aagcggcccg gcgggacggg ggcgcttgcc
     1141 cccgccggcc gccgggccgc gcgggattac atcttttcgg gcgccgtgat cttcagcatg
     1201 gtcaggccgc cggcgatggt gttctttgcc gccacacaga gggccagccg cgcctgttgc
     1261 agcgcgtcgg gctcgccctt gacacggcag gcggtgtaga atttgtggaa cagggtcgcc
     1321 agctcggtga catagcgggt gatgcgggcc gggtcgtagt ttttggccgc ggtcacgatc
     1381 tcctcgggga aatagccgat gtggcgcacc agctcgatct cggccgggtc ggtgagcagc
     1441 gagaggtcgg cctcggccgc aggccgcggc gtgacgccct cctccgccag ggcccgcagg
     1501 atgctgcaga tgcgggcgtg ggcgtactgc acatagtaca ccgggttgtc gctgtcctgc
     1561 tttaccgcca ggtccatgtc gaacagcacc tgggcccccg gctcgcgctg gttgaaaaag
     1621 aagcgggcgc tgtcgatggg cacctcgtcc agcaggtcca ccagcgagac cgccttgccg
     1681 gtgcgcttgc tcatgcgcac ctcctggccg tcccgcacca gcttgaccag ctgcatcagc
     1741 accacatcca gccggctgcc ggacaggccc accgcgtcca tcgagccttt catgcgcggc
     1801 acatggcccg cgtggtcggc gccccagacg tcgatggcca ggtcgaagcc gcgggtgacc
     1861 agcttgttgt agtggtaggc gatgtcggcg gcgaaatagg tggggatgcc gttggcgcgc
     1921 accagcacct cgtccttggg gccctcctgc acctcgccct cgcccgcggc ggccttgggg
     1981 cgcttgttgg cgccccattt gaggctgtat tcccccgcct tgtaccagat ggcgccgtcc
     2041 ttttcatagg tggcccccag ggcggagagc ttgtccagca cgtcctgcac cgcgccgctc
     2101 tcgtgcagct ggctctcgag gaaccagacg tcgtagtgga tgcggtattt ttccatggcg
     2161 tctttcatgg cctggatgtt cagcggcagg gcgtagtcca ccagggccct tttgcgctcc
     2221 tcctcggggc ggtccacata ggcgtcgccg tgcttgtccg caaactcgcg ggcgcgctgg
     2281 cggatgtcct cgccctggta gcccttttcg gggaagggca ccgcctcttc tcccttgaag
     2341 atctgcagat agcgggcgga caggctgtcg ccgaattttt cgatctggtt gcccgcgtcg
     2401 ttgacgtaga actcgcgggt gacctggtag cccgcccact ccagggccgc ggccaggcag
     2461 tcgcccagcg cgccgccgcg ggcgttgccc aggtgcatgg ggccggtggg gttggcggag
     2521 acgaactcca cgttcacccg cttgcccgcg ccgaaatcgg tgcggcccca atcgctgccc
     2581 gcctggtttg cagccagcac cacctattca aaatagcggt gggacaaaaa gaagttgatg
     2641 aagccggggc ccgccacctc cagccgctca aagggggtgc cggcgcagtc ggcgcgggcg
     2701 gccagaatct cggcgatgcg gcgcgggggc aggcggaacg cctttgcccc caccatggcg
     2761 gcgttgcagg cgaagtcgcc gttgcgcacg tcgccgggga tctcgatttt gaacgcgggg
     2821 gcggccgcct ggggcagctc gcccgccgcc acggccgcgc ccatggcggc caggatcaag
     2881 tcgtaggcgg cgtcgcgcgc gcgggccacc gggttgtaat gatgaaactc caaagtcgtt
     2941 atgcctccct gacggtaata ttgacggtgt ttttggaaaa caggctcgag ttgatgtcca
     3001 gattatagct gaagcgcagg ctgccgcccc gctcggacaa atcggcgacg atctcgtcgg
     3061 cggcgatgcc catggtgatg gcgccgtggc cggtgtcgta gtggcagatg tgccgccgcc
     3121 ccttttcgat caccagctgg ctgggcgcgg ggccgaaccg cagcatgctg accgtgccgg
     3181 ccttgtccag ctttacggtg gtggtacagc cgtcgtagcc ggtggcctcg gtctcgcggt
     3241 aggtgatgta gtagctgccg ccccgctgca caaagctgcc gcgggtcatc agctccaccg
     3301 cgttctcctc gccctccgac tcctgtatcc ccttgatcct gatcagataa tcctctttca
     3361 tatccgccct cccgggtctg acccggacgg cgcgggcggg ccgcccgcgc cgctccggca
     3421 tccctgtgct caatcccata gtgtaaacgg gtgcacggtg ccgtgcacat cctttccgag
     3481 gaaaaggccg cccacctcgg cgaagttctc ccccgaatcg ctgacataaa agctgacggc
     3541 gccgccctcg gcgcggccgt tcagcaggcc ctgccgctcc agcagccgcc gcagcgccgc
     3601 ggccccctcg gcgcccgagt cgatcagccg cacccccggg cccatctcgt cggcgatggc
     3661 gccggcgatg agcgggtagt gggtgcagcc caggatcagc gtgtccaccc ccgcctgccg
     3721 cactggggcc aggtactcct ttgccagggc cgtgacgatg gggtcgcggc ggcccacata
     3781 gccgttttcg atcaccggca caaacagcgg gcaggcccgg gccgtgaggg cgctgtccgg
     3841 ccgcagggcg cgcacggcgg cctcatagcc gccgctgcgg atggaggccg ccgtggcgat
     3901 gaccccgatg cggccgctgc cgctggcctc cgccgccgct gccgccgccg cctgcaccac
     3961 ccccacaaag ggcacgggca gcgcctcggt caccgcggcg ggcagggtgg aggagacggt
     4021 gccgcaggcg cagagcaccg ccttgacccc ctttgagagc aaaaactgca catcctcttt
     4081 gccgtagcgg atgatggtgt cggcgctgcg gccgccgtag ggcacccgcg ccgtgtcgcc
     4141 gaagtagacg atgtcctcgc cgggcagcag ccgcaccagc tccttgaccc cggtcaggcc
     4201 gcccaggccg gagtcgaaca cgccgatggg ccggttatcc attgggggtc tcctttcgct
     4261 caaaggcgag ctggatcagc cggtccagca gaccgccgta gctcatgccc gcgtgcagca
     4321 tcagcttggg atacatgctg atgtcggtga agccgggcat ggtgttgatc tcgttgagca
     4381 gcacccgccc ggtgcccgcc tccacgaaaa agtccacccg ggccaggccg gtgcagcaga
     4441 gggtgcggta ggcccgcacc gcggtcgccc gcacctcctc cagcttttca ggggagagct
     4501 gcgccgggat gaccacccgg ctggtgccgg cgatgtactt gtcgtcgtag gtgtaaaact
     4561 cctggctggc gaggatctcg ccgggcaggg tgccctgcgg cccgtcgttg cccatcaccg
     4621 cgcactccac ctcctgcccg gtgacgaacc gctcaaagac gatgcgggtg tcgtgggcca
     4681 gggccagg
//
LOCUS       DBMDYA010000143         4667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4448_length_4667_cov_1.388305, whole genome shotgun sequence.
ACCESSION   DBMDYA010000143 DBMDYA010000000
VERSION     DBMDYA010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_4667_cov_1.388305"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            693..1346
                     /locus_tag="ACZPFK_04770"
     CDS             693..1346
                     /locus_tag="ACZPFK_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405656.1"
                     /translation="MNIRKVRIVSICFVFLLLAVSAGIWFLKTTQDTQNQRKEYAEIF
                     EAYAPFGLFYSEKENRLYYGGELVRYFEDLISTEHYLKWPNQDGTVDVYVERNVSGEL
                     IGIKSFNQQEFLGRTSSLKTAGHDLEITINIDGYTSDVEEMVEKLIAEEYEIYRQYGL
                     IYDMDRGRLYLDGELVGYFEDKELNHYFGPYEDSTIKIYAMRDKNGDLSGLDVRDTP"
     gene            complement(1818..2126)
                     /locus_tag="ACZPFK_04775"
     CDS             complement(1818..2126)
                     /locus_tag="ACZPFK_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405657.1"
                     /translation="MNYIDEIFLRADIQQLRMFLLYGVDGDPDPRPYQERLDSAQKPM
                     TARFQQEYPDMEKYEEMTGLVYNYVGAIEEVYMEIGLQVGALLAAQVGQNLKTAFEKR
                     "
     gene            2306..2518
                     /locus_tag="ACZPFK_04780"
     CDS             2306..2518
                     /locus_tag="ACZPFK_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405658.1"
                     /translation="MGTMTKKINMLLIEMGLTKQELAKRLGTTPSNLSGKLKRDNFSE
                     LELQEIAAACGATFEGKFITDTGKQI"
     gene            complement(2679..4415)
                     /locus_tag="ACZPFK_04785"
     CDS             complement(2679..4415)
                     /locus_tag="ACZPFK_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR1405659.1"
                     /translation="MSSKPYKGFSPKWYTEKPPAGSYRSILKWGDPEFNKVPKETLYK
                     MIKDTFHLTDEDFSQYRELGLEQVKFDLPTGLSDEQLARFAAIVGADNVKTDDYSRLS
                     VAYGKTMYDLLRLRKRIVENVPGAVLYPENKEQIQQIVTYCDEQRIPVYVYGGGSSVT
                     RGVEAMKGGVTLDMRRHFNKVLDFNETDQTITVEAGMSGPQLEEVLNHAQQRLGAHRA
                     YTCGHFPQSFEYSCVGGWVVTRGAGQNSTYYGKIEDIVLGQEYATPVGTVKTDAYPAR
                     ATGPSINQIMMGSEGTFGVLTAVTLKVFHLTAENHLKFSFMFKSWTDAQAAAREIMQG
                     QFGYPSVFRLSDCEETEIMMKLYGIEETPLNSLLRMRGFTEGNRCLFLGFTDGERGFC
                     KNIVKNVKRICHRHGAMYLTGIPTTGWEHGRFNDPYLRDTMQDFGIMTDTLECTVNWS
                     NMQQVYETVRGYCKSRPDTICMTHMSHAYPQGANLYFIFIARMTELEEFTEYHRGILD
                     HIQKSGAAISHHHGVGKLFAPWLEGYLGSNQMDILRALKAHFDPHGIMNPGGTIGLDL
                     PEEEKRYLRPKW"
     gene            complement(4412..>4667)
                     /locus_tag="ACZPFK_04790"
     CDS             complement(4412..>4667)
                     /locus_tag="ACZPFK_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019507.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1405660.1"
                     /translation="APTRSLTTTVATTLPFCPVLPVKTAGELPKGALLRAMTEINSFK
                     LTHPARRGDVLIDDLLGTGVALVATADTGSYEQASEEETR"
BASE COUNT         1050 a         1166 c         1356 g         1095 t
ORIGIN      
        1 ccggcgcggg cgggaggcgg agataaaaaa tagccgcagg ctattttttc ggccatccgg
       61 cccgtcccgg gccggggcca taggaataag tcgtttttat tgcgcagcaa taaaaatagc
      121 cgcaggctat tttttcggcc atccggcccg tcccgggccg gggccatagg aataagttgt
      181 ttttttattg cgcagcaata aaaatagccg caggctattt ttttcggcca tccggcccgt
      241 cccgggccgg ggccataaga ataagtcgtt tttattgcgc agcaataaaa atagccgcag
      301 gctatttttt cggccatccg ttttggttgc cgcggcagcg gcgagaaacg cggcccaagg
      361 aggttatgat gtgagtatga aaagctgact gtgactgaga agcgctgcag gtgcggccat
      421 tgcaaaaagg gtgatggatt tgtcgttgag aatacaggtc ctccggtctg ttacgaactc
      481 tgaaacgcga tttatccaat ggtctatgcc tttcaaaacg gagctgacct cgactatggt
      541 agggtcggag aaaaatgttt cgatgcgaaa tgcccaggcg agggaagagt tatggtccgt
      601 ggggaggtga tcggttcaca catccagctg ttgagggtgg aaaattaaaa gttgtaagct
      661 acaaaataga agtagcgaaa gagagataca agttgaatat tagaaaagtc agaatagtgt
      721 ctatttgttt tgttttttta ctattagctg ttagcgccgg tatctggttt ctaaagacaa
      781 cacaagatac acaaaatcag agaaaagaat acgcagaaat ctttgaagca tatgcgccct
      841 ttggattatt ttatagcgaa aaagaaaaca ggctgtacta tgggggcgag cttgttcgct
      901 attttgagga ccttatcagt acggagcatt acttgaagtg gcccaaccag gatgggacgg
      961 tagatgtgta tgtggaacga aatgtatccg gagagcttat cggcataaaa tcttttaatc
     1021 aacaggagtt tttaggcaga acttcatctt tgaaaacggc tggccatgac ttggaaatta
     1081 cgatcaatat agatggttat acgagtgatg ttgaagagat ggtagaaaaa cttattgcag
     1141 aagaatatga gatttacagg caatatggct taatctacga tatggaccgt ggccgcctgt
     1201 atttggatgg ggaacttgtg ggatattttg aagacaaaga gttaaaccat tattttgggc
     1261 cgtatgaaga cagcacaatt aaaatatatg ccatgcgaga taaaaacggg gatttatccg
     1321 gattggatgt ccgtgatacg ccctgattct aggctgcaac tttcagtttg ttgaactgat
     1381 ttgtttcgca ttcttgatat aagacgcgct tttacgttag ccgttttcaa tggtagacca
     1441 gctaagcaag gtcaagcaag agattggacg gtaaagagca gcaaaaacag ggcaaaacaa
     1501 caaggcccgg cggtaagacg gcccagatta gaagcggagg ctacttttct gccatccgct
     1561 ttggtttccg cggcacagcg gggaaagccg ggggcgggac aagccgcgct ctctcactgc
     1621 ggcaataaag gacaaagggg aaagacgctt tgcggatgcg cctttcccca tttgttttgt
     1681 ccgcacgggg agagagcggc gaggaacagg gcggaggaaa aaaggcatgc ggctcaaaag
     1741 agggccgcgg caggccggcg ccgcaacgcg cggctgtgct taggggctgt gccccaggcc
     1801 cctggctttg ccgggagcta tcttttttca aacgccgttt tcagattttg gccgacctgt
     1861 gcggccaaca gcgcgcccac ctgcaggccg atctccatat aaacctcctc gatcgcaccc
     1921 acatagttgt agacgagccc ggtcatctcc tcgtattttt ccatatccgg atattcctgc
     1981 tgaaagcggg cggtcatggg cttttgggcg ctgtcgagcc gctcttgata agggcgtggg
     2041 tccgggtcgc cgtccacgcc gtacagaaga aacatgcgaa gctgttggat gtccgccctc
     2101 aaaaaaatct catcgatgta gttcatgaaa ttttcgcacc tccgtttttg tttaaaaaat
     2161 aaattaattt attttataat aaaatattaa atttaaaaac gaaaaatgtc aatactaaat
     2221 tttcactctt gagtaaaaat attgacttgg cttttgattt tttcaattgt gtaatgtata
     2281 atttgacggg ataggaggcg aatcaatggg cactatgaca aaaaagataa atatgctttt
     2341 gatagaaatg ggactcacaa agcaagagct ggcaaagcga ttggggacga caccttccaa
     2401 cctttcggga aaactgaaga gagacaattt ttcagaattg gagctgcaag aaatcgcggc
     2461 agcctgcgga gccacttttg aggggaagtt tatcaccgat accggcaagc agatataaaa
     2521 aatagccagg ggctattttt ttcggccatc cgtttcggtt gctgcgcagc ggcgagaaac
     2581 ggggccatga agataaattg ttttttattg cggagcaata aaaaagacct ccgagaaacg
     2641 gaggtctttt ttatatctgt tctggctgcc gccccggctc accacttggg gcgcaggtag
     2701 cgcttttcct cctccggcag gtctaggccg atggtgccgc ccgggttcat gatgccgtgg
     2761 gggtcgaaat gcgctttcag cgcgcgcagg atgtccatct ggttggagcc gagatacccc
     2821 tccagccagg gggcgaacag cttgcccacc ccgtggtggt gcgagatggc cgcgccgctc
     2881 ttttggatgt ggtccaaaat gccccggtga tactctgtaa actcctccag ctcggtcatg
     2941 cgggcgatga agatgaagta gaggttcgcg ccctgaggat aggcgtggct catgtgggtc
     3001 atgcagatgg tgtccgggcg gctcttgcaa tagccgcgca ccgtctcata cacctgctgc
     3061 atgttgctcc agttcaccgt gcactccagc gtgtcggtca tgatgccgaa atcctgcatc
     3121 gtgtcccgca gataggggtc gttgaagcgc ccgtgctccc agccggtggt ggggatgccg
     3181 gtcaggtaca tggctccgtg ccggtggcag atgcgcttga cgtttttgac gatgtttttg
     3241 caaaagccgc gctcgccgtc ggtaaagccc aaaaacaggc agcggttgcc ctcggtgaag
     3301 ccgcgcatcc gcagcaggct gttcagcggc gtctcctcga tgccgtacag cttcatcatg
     3361 atctcggtct cctcgcagtc ggacaggcgg aacaccgacg gatagccgaa ctgcccctgc
     3421 atgatctccc gcgccgccgc ctgggcgtcg gtccagctct tgaacataaa gctgaatttc
     3481 aggtggtttt cggcggtgag atggaacacc ttcagggtca cggcggtgag cacgccgaag
     3541 gtgccctcgc tgcccatcat gatctggttg atggaggggc cggtggcccg ggccggatag
     3601 gcgtcggtct tgacggtgcc cacgggggtg gcgtactcct gccccagcac gatgtcctcg
     3661 atcttgccgt aataggtgct gttttgcccg gcgccgcggg tgaccaccca gccgcccaca
     3721 cagctgtact caaagctctg cggaaaatgc ccgcaggtat aggcgcggtg cgcgcccagg
     3781 cgctgctggg cgtggttgag cacctcctcc agctgcgggc cgctcatgcc cgcctccacc
     3841 gtgatggtct ggtcggtctc gttgaagtcc aataccttgt tgaagtgcct gcgcatgtcc
     3901 agcgtgacgc cgcctttcat ggcctccacg ccgcgggtga cgctggagcc gcccccgtaa
     3961 acgtacaccg ggatgcgctg ctcgtcgcag taggtgacga tctgctggat ctgctctttg
     4021 ttctccgggt agagcaccgc gccgggcacg ttctccacaa tgcgcttgcg cagccgcagc
     4081 agatcgtaca tggtcttgcc gtaggccacc gagaggcggc tgtagtcgtc ggttttgacg
     4141 ttgtccgccc ccacaatggc ggcaaagcgg gccagctgct catcggaaag cccggtgggc
     4201 aggtcgaact ttacctgctc cagccccagc tcgcggtatt ggctgaaatc ctcgtcggtc
     4261 agatgaaagg tgtccttgat catcttgtac agcgtctctt tgggcacctt gttgaactcc
     4321 gggtcgcccc atttcaggat gctgcggtag ctgccggcgg gcggcttttc ggtgtaccat
     4381 ttcggcgaaa accccttgta cggcttgctg ctcatctcgt ctcctcctcc gatgcctgtt
     4441 catagctgcc ggtgtccgcg gtggccacaa gggccacgcc ggtgcccagc aggtcgtcga
     4501 tcaaaacgtc cccgcgccgc gcggggtggg tcagtttgaa gctgttgatc tcggtcatgg
     4561 cccgcagcag cgcccccttg ggcagctcgc ccgccgtctt caccggcagc accgggcaaa
     4621 agggcagggt ggtggccacg gtggtggtca ggctgcgggt gggcgcg
//
LOCUS       DBMDYA010000144         4621 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4488_length_4621_cov_1.540265, whole genome shotgun sequence.
ACCESSION   DBMDYA010000144 DBMDYA010000000
VERSION     DBMDYA010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_4621_cov_1.540265"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="ACZPFK_04795"
     CDS             complement(<1..337)
                     /locus_tag="ACZPFK_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1405661.1"
                     /translation="MSSELEKRFLSLRKQVIEASFGRLNPVQRDAVFTVNGPLLILAG
                     AGSGKTTVLVNRIANLVRYGTAHDSDKVYGAVTEEDVAALEALLKDGGKLTARLAGLL
                     SVDAVRPWHV"
     gene            complement(540..1721)
                     /locus_tag="ACZPFK_04800"
     CDS             complement(540..1721)
                     /locus_tag="ACZPFK_04800"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR1405662.1"
                     /translation="MQGFLDAVVGIARESGFAGIIENPGYLIMIIIACVLLYLAIVKQ
                     FEPLLLLPIAFGMLLANLPGSGLIHMEFFLTESGHPDFLNVLNNGGLADMLYLGVKLG
                     IYPPLIFLGIGTMTDFGPLISNPKSLLLGAAAQLGIFMTYFGAKMLGFTAQEAGAIGI
                     IGGADGPTAIYVTTKLAAHLLGPIAVAAYSYMALVPVIQPPIMKLLTTKKERQIKMTQ
                     LRPVSKTEKIIFPIMCTILISLILPSATPLIGMLMLGNLMKESGVVERICKTAGNELM
                     NIITIFLGFSVGCTTSADTFFTAQTLFIILLGVIAFGFGTAGGVLLGKLMCWATHGKV
                     NPLIGSAGVSAVPMAARVSQKVGQQEDPSNFLLMHAMGPNVAGVIGSAVAAGILINML
                     G"
     gene            complement(1740..2105)
                     /locus_tag="ACZPFK_04805"
     CDS             complement(1740..2105)
                     /locus_tag="ACZPFK_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1405663.1"
                     /translation="MEPSIATVIITGLVLVFAILVLLYLIITLQGKIFVSIDNKKKAK
                     TEAAKAAPAPAAKAAPAAPAASAPVVEAGISGEVVAAISAAVAAVSEGPVTVRSIAKA
                     PARKNGWGLAAVYSYTEPF"
     gene            complement(2353..3378)
                     /gene="mreB"
                     /locus_tag="ACZPFK_04810"
     CDS             complement(2353..3378)
                     /gene="mreB"
                     /locus_tag="ACZPFK_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1405664.1"
                     /translation="MAQNDIGIDLGTTSVVIYVEGKGIVLSEPTVVAVDTDTNRVLAV
                     GDDAYRMVGRTPAHIMAVRPLKDGVISDHVLTREIVRRFLVKAYQSPVVKPRVAVCVP
                     AAISGIESDAAVEAVVAAGARQVFLIDEPIAAALGAGIDITRPRGHLVVDIGGGTSDI
                     AVISMGGKVQSSSIKVAGNTLDTAIVRAVRDKFKLAIGEKTAEILKRTVGCCAPEAGL
                     SSSMEVKGRSLENGLPKRQTVTTEDLYETIGECVAELASAVHSVLERTPPELAGDIYT
                     EGMLLTGGGALLPGLDLYMSQRLKIRVQAAEDPVNCVARGTGKSLSMADRLESGFRDA
                     TPGIGKR"
     gene            complement(3365..4087)
                     /locus_tag="ACZPFK_04815"
     CDS             complement(3365..4087)
                     /locus_tag="ACZPFK_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012383.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1405665.1"
                     /translation="MKKAAAVLKNLLFPRRCPYCGQVVGFAPACGCAPRVEACRLCGP
                     AALLDGEGRRLAFLDGGAACYAYTGPIREGGRRLKFAGASGLAALYGREMAAKLSSLL
                     PDERFDGVVPVPSTRKERRRRGYDVPLLLARALSEALAIPLWDDILVKEFETKRQHEL
                     PLAERKGNLLGAFGLRGDAALSGRRVLLCDDVATSGATLDECAKTLKAGGAATVWGVV
                     FASTPRQEARKAKEREQRHGAE"
     gene            complement(4108..4395)
                     /locus_tag="ACZPFK_04820"
     CDS             complement(4108..4395)
                     /locus_tag="ACZPFK_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HIR1405666.1"
                     /translation="MLTYSGESIRELEIAYAVTIHKSQGSEFGAVVLPVSADTPKRLC
                     YRNLIYTGVTRAKQLLVLAGSRGTLHAMIENDRKMLRYSCLTRLLQDESVC"
     gene            complement(4451..4588)
                     /locus_tag="ACZPFK_04825"
                     /pseudo
     CDS             complement(4451..4588)
                     /locus_tag="ACZPFK_04825"
                     /inference="COORDINATES: protein motif:HMM:NF037213.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          803 a         1545 c         1451 g          822 t
ORIGIN      
        1 gcacgtgcca gggccgcacc gcatccaccg acagcaggcc cgccagccgc gccgtcagct
       61 tgccgccgtc cttcagcagt gcctccagcg cggccacgtc ctcttcggtg acggcgccgt
      121 agaccttgtc gctgtcgtgg gccgtgccgt accgcaccag gttggcgatg cggttgacca
      181 gcaccgtggt cttgccgctg cccgcgcccg ccaggatcag cagcggcccg ttgaccgtga
      241 agaccgcgtc ccgctgcacc gggttgagcc tgccgaagct cgcctcgatc acctgtttcc
      301 tgagagataa aaaccgtttt tccaattcgc tgctcatccg acaccgccta aactcttttt
      361 tggccccgcg ccgcgcccgg gacccccgcg cgcagcgcct aacaatgcaa aatcaaaatt
      421 attataccac actccggccc ggaggaaaag ggctgcggcg ccgatgacca cccggctgag
      481 gatccaaaac gacaaaattc ccggacagag agctgtccgg gaatttttca ggctgggcgt
      541 cagcccagca tattgatcaa aataccggcg gccacagcgc tgccgatgac gcccgccacg
      601 ttggggccca tggcgtgcat gagcaggaag ttggagggat cctcctgctg acccactttc
      661 tgggagacac gggccgccat cgggacggcc gaaacgcccg ccgagccgat gagggggttg
      721 accttgccgt gggtggccca gcacatgagc ttgcccagca gcacgccgcc cgcggtgccg
      781 aagccaaagg cgatgacgcc gagcaggatg ataaagaggg tctgagcggt gaagaaagtg
      841 tccgcgctgg tggtgcagcc caccgagaag ccgaggaaga tggtgatgat gttcatcagc
      901 tcgttgcccg cggtcttgca gatgcgctcg accacgccgg actctttcat caggttgccc
      961 agcatcagca tgccgatcag cggcgtggcg ctgggcagga taagcgagat gaggatggtg
     1021 cacatgatcg ggaagatgat cttctcggtc ttggagaccg ggcgcagctg cgtcatcttg
     1081 atctggcgct cttttttggt ggtgagcaat ttcatgatgg gcggctgtat cacgggcacc
     1141 agggccatat agctataagc cgcaaccgcg atggggccca gcaggtgcgc cgccagcttg
     1201 gtggtgacat agatggcggt cgggccgtcg gcgccgccga tgatgccgat ggcgccggcc
     1261 tcctgggcgg taaagcccag catcttggcg ccgaagtagg tcatgaagat gcccagctgg
     1321 gcggccgcgc ccagcagcag gctcttgggg ttggagatca gcgggccaaa gtcggtcatg
     1381 gtgccgatgc ccaggaagat cagcggcggg tagatgccca gcttgacgcc taagtacagc
     1441 atgtccgcga gaccgccgtt attgagcacg ttgagaaaat ccggatgccc cgactccgtc
     1501 aggaaaaact ccatgtggat gaggccggac cccggcaggt ttgccagcag catgccgaaa
     1561 gcgatgggca gcagcagcag cggttcgaac tgtttgacga tcgcaaggta cagcagtacg
     1621 caggcgatga tgatcatgat caggtaaccc gggttctcaa tgatgcccgc gaagccggat
     1681 tcccttgcga tgccgacgac ggcgtctaaa aatccctgca tggttaatcg tcccctctct
     1741 taaaatggtt cagtgtagga atacactgcc gcaaggcccc agccgttctt ccgcgcgggc
     1801 gccttggcga tgctgcgcac ggtgaccggc ccctcggaca cggcggctac ggcggcgctg
     1861 atcgccgcga cgacctcgcc ggagatgccg gcctccacaa cgggggcgga ggctgcgggg
     1921 gctgcgggcg ccgccttggc cgcgggggcg ggggctgcct tggcggcctc ggtcttggct
     1981 ttctttttgt tgtcaatgct cacaaagatc ttgccctgca gggtaatgat caaatagagc
     2041 aataccagaa tggcaaagac cagaaccaaa cccgtgatga taacggtagc aatacttggc
     2101 tccatttcag atccctcctt aaaagcgata tctttctgta ttataattga tttgagattc
     2161 ggtttcaaca ggcaaaacca caaatcgtta attttttggc aatatttttt ctataaaacc
     2221 ctgcgcgccg gcggacgctt ttttcgcttt ttccggcctg cgggcatccg cccgcagcaa
     2281 tgcaaagcgg tggcgagccg gggcgctttt ttaacgaaaa aggggcgggc cgcagcccgt
     2341 ccctttttgt ccctatcttt tgccgatgcc gggcgtcgca tcccggaagc cggattccag
     2401 ccggtccgcc atggagaggc ttttgccggt gccgcgggcc acgcagttga ccgggtcctc
     2461 ggcggcctgt acccggatct tgaggcgctg cgacatatac aggtcgagac ccggcagcag
     2521 cgcgccgccg ccggtgagca gcatgccctc ggtgtagata tcccccgcca gctcgggcgg
     2581 ggtgcgctcc agcacgctgt ggacggcgga cgccagctcg gccacacact ccccgatggt
     2641 ctcgtagaga tcctcggtgg tcacggtctg gcgtttgggc aggccgtttt ccaggctgcg
     2701 ccccttgacc tccatcgagg aggagagtcc cgcctccggc gcgcagcagc ccaccgtccg
     2761 cttgaggatc tcggcggtct tctcgccgat cgccagcttg aacttgtctc tcaccgcgcg
     2821 cacgatggcc gtgtccagcg tgttgccggc caccttgatc gagctggact gcaccttgcc
     2881 ccccatcgag atcaccgcga tgtcgctggt gccgccgccg atatccacca ccagatgccc
     2941 ccgcggccgg gtgatgtcga tgcccgcccc aagggcggcg gcgatgggct cgtcgatcag
     3001 aaagacctgc cgcgcgccgg ccgccaccac cgcctccacc gcggcgtcgc tctcgatgcc
     3061 cgagatcgcc gcgggcacgc acaccgcgac ccgcggcttc accaccgggg actggtaggc
     3121 cttgacgaga aagcgccgca cgatctcgcg ggtgaggacg tggtcggaga tgacgccgtc
     3181 cttgagggga cgcaccgcca tgatgtgggc cggggtgcgg cccaccatgc ggtaggcgtc
     3241 gtcccccacc gccagcaccc ggttggtatc ggtgtccacc gccaccacgg tgggctcgct
     3301 gagcacgatg cccttgccct ccacatagat caccaccgag gtagtgccga ggtcgatccc
     3361 gatgtcattc tgcgccatgc ctttgctccc tctctttcgc tttccgcgcc tcttgccgcg
     3421 gtgtggacgc gaacaccacg ccccacaccg tagccgcgcc gcccgccttg agcgtcttcg
     3481 cgcactcgtc cagcgtcgcg ccgctggtgg ccacgtcgtc gcacagcagt accctgcggc
     3541 cggacagagc ggcgtccccc cgcaggccga acgcccccag caggttgcct ttccgctcgg
     3601 ccaggggcag ttcgtgctgg cgctttgtct cgaattcttt taccagtata tcatcccaaa
     3661 gcgggattgc aagcgcctcg gagagcgcgc gagccagcag cagcggcacg tcgtagccgc
     3721 ggcggcggcg ctcttttctg gtcgagggca ccggcacgac gccgtcaaag cgctcgtccg
     3781 gcaaaaggga cgacagcttg gccgccatct cgcgcccata aagcgcggcc agccccgacg
     3841 cgcccgcaaa cttcagcctg cggcccccct cccggatggg gccggtatag gcataacagg
     3901 ccgcgccccc gtccaggaac gcgagcctgc ggccctcccc gtccagcagg gccgccgggc
     3961 cgcagaggcg gcaggcctcc acccggggcg cgcacccgca agcgggcgca aaccccacca
     4021 cctgcccgca ataggggcag cgccggggga aaagcaggtt ttttaaaaca gccgccgcct
     4081 ttttcatttt gtccctcccc gccgcgctca acagacgctc tcgtcctgca gcagccgcgt
     4141 caggcaggag tagcgcagca tcttgcggtc gttctcgatc atcgcgtgca gggtgccgcg
     4201 gctgccggcc agcacgagca gctgctttgc ccgggtcacg cccgtataga tcaggttgcg
     4261 gtagcacagc cgcttgggcg tgtcggccga caccggcagg accaccgccc caaactcgct
     4321 gccctggctc ttgtggatgg tgaccgcata ggcgatctcc agctcgcgga tgctctcgcc
     4381 ggagtaggtc agcagcctgc gggtccaccg cctctatcac gcccaggtcg ccgttgaaag
     4441 cgcccacgcc gccctcgccg tcctcccgct cgtagaggat gtcatagttg ttgcgcacct
     4501 gcatgacctt gtccccctcg cgataggtga cgccgaagta ggtgagctgc cgcttgcccg
     4561 gcgcgggcgg gttgagcagc tgctgcagcc gcgcgttgag cgccgcggtg ccggtggggc
     4621 c
//
LOCUS       DBMDYA010000145         4591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4536_length_4591_cov_1.323163, whole genome shotgun sequence.
ACCESSION   DBMDYA010000145 DBMDYA010000000
VERSION     DBMDYA010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_4591_cov_1.323163"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="ACZPFK_04830"
     CDS             complement(<1..204)
                     /locus_tag="ACZPFK_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405667.1"
                     /translation="MRVLKKILLWLLMVLVLAALVVGGTVGYFYYKAEPGDAAAAPLT
                     VQTGAEGDAAVEHRFVTPVGYSLD"
     gene            complement(467..817)
                     /locus_tag="ACZPFK_04835"
     CDS             complement(467..817)
                     /locus_tag="ACZPFK_04835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1405668.1"
                     /translation="MEVKRGEVFYADLSPVVGSEQGGTRPVLIIQNDIGNRYSPTVIA
                     AAITSRQDKNKMPTHINIKGEKCGLAKDSIVLLEQVRTLDKRRLHECTGRIDETDQRR
                     VDEALNVSFGLTSF"
     gene            complement(1022..1390)
                     /locus_tag="ACZPFK_04840"
     CDS             complement(1022..1390)
                     /locus_tag="ACZPFK_04840"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR1405669.1"
                     /translation="MLQLGFDAARVSRLEKSMGNPRFCERVFSAQENALIAAKPRPAE
                     TAAAHFAAKEALGKALRCGIFGFDLRQAAVLRRPDGSPYFAFSGALAERMERQGLAAE
                     VSLSHEGGYAFACVLLQPKE"
     misc_binding    1818..2055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2135..3154
                     /gene="pheS"
                     /locus_tag="ACZPFK_04845"
     CDS             2135..3154
                     /gene="pheS"
                     /locus_tag="ACZPFK_04845"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1405670.1"
                     /translation="MREALEKIRQSAEELLAKADSEAALEELRVRFLGKKGEVTAILK
                     QMGSLSAEERPKIGALANEVRAAIDAEVRQRGKELAAGLLEQRLAAERIDVTLPGRRP
                     VQGRRHPFQQVLDELKEIFLGMGFEVKAGPEVEYDHYNFEMLNMPKSHPARDTQDTFY
                     ITENILLRTQTSPMQIRTMEAQKPPIRIICPGRVYRSDAVDATHSPLFHQIEGLVVDK
                     DITFTDLKGTLEAFIKKLYGEETRVRFRPHHFPYTEPSAEMDMSCFKCGGKGCSLCKG
                     EGWIEILGCGMVHPQVLENCNIDPEVYSGFAFGVGLERIAMMRYGIDDMRLFYENDLR
                     FLKQF"
     gene            3200..>4591
                     /gene="pheT"
                     /locus_tag="ACZPFK_04850"
     CDS             3200..>4591
                     /gene="pheT"
                     /locus_tag="ACZPFK_04850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1405671.1"
                     /translation="MRLPLNWVRDYADIVTTPHEYAEKMTMSGSKVEGYTCEADGIKN
                     VVIGKILKLERHPDSDHLWVCTVDVGQEAPLTIVTGAQNLKEGDLCPTALDGSTLPGG
                     VKIRRGKLRGVESCGMLCSLGELGLTTHDFPSAVEDGIMVLDEEVPVGTDAVKALGMD
                     DVSVEFEITPNRPDCLSIRGLARESAATYGVPFRDHLPAAPAGEGDIRAWLSVEITAP
                     GCSRYVAAMVTNVRVKSSPRWLRERLRLCGVRPINNIVDITNYVMLEYGHPMHAFDHA
                     YVGGKHIHVRMAREGEKIVTLDGVERNLDPTMMVIADDKVPMAVAGVMGGEFSGIYDN
                     TKTIVFEGACFDGPSVRVTAKKVGLRTEASGRFEKGLNPENCLPAVRRALELVAELDA
                     GDVIGGIIDVYPQPRAEHRIPLRPGAVNRLLGTDVPVDEMIAILKPLGFGIEKKPLTA
                     LVEHEESPYVVVVP"
BASE COUNT          837 a         1554 c         1396 g          804 t
ORIGIN      
        1 atccaggctg tagcccaccg gcgtgacgaa ccggtgttcc accgcggcgt cgccctccgc
       61 gccggtctgc accgtgagcg gcgcggccgc cgcgtccccc ggctcggcct tataataaaa
      121 gtatcccacc gtgccgccca ccaccagcgc cgccagtacc agcaccatga gcagccagag
      181 caaaatcttt ttgagtaccc gcatccgtct cacctgtttc tcttttgtct tggccccaat
      241 gggggccctg caatccccgg ccccgccggc cacaaagtgc acggcccgcg aacggcccgc
      301 gcccagcgcg cggcaagctg ttttgccatg gctacagtat agcaaaaaaa agcgtatttt
      361 gaaagtttct gtatgagaaa cgccgcgggg aggctttttg ttgctctccc cgcggcgtcc
      421 gtccttgctt atcatttacc cgttttccgg gcctgtcctc aaagcgctaa aagctggtca
      481 gcccaaagct gacgttcagc gcctcgtcca ccctgcgctg gtcggtctcg tcgatgcgcc
      541 ccgtgcactc gtgcaggcgg cgcttgtcca gggtgcgcac ctgctccagc agcacgatgc
      601 tgtccttggc aagaccgcat ttttcccctt tgatgttgat gtgcgtcggc attttgttct
      661 tgtcctgccg cgaggtgatc gcagcggcga tcacagtggg gctgtaccga ttgccgatat
      721 cattttgtat aataagtacc ggccgggtcc cgccctgttc gctgcccacc acggggctca
      781 ggtccgcata aaagacctct cctcgtttga cttccatcct gaaactcgcc ctcctgtgca
      841 aaataggtga attttttcct tgcaagcttg cacccttatt ttgcaccgga aaaagccggt
      901 ttaaacatcg ctttttatct tttgcccaaa aggccgccgc ccaccgaaaa gcccgcgccg
      961 gggcgccgcc cggcgttttt ccgcccccgc gctccagccc gcccgccgcc gggccgcgtc
     1021 cttactcctt gggctgcagc agcacgcagg caaaggcgta gcccccctcg tgggagagcg
     1081 agacctccgc cgccaggccc tgccgttcca tccgctccgc cagcgcgccg ctgaacgcaa
     1141 aataaggcga cccgtcgggc cgccgcagca ccgcggcctg acgcaggtca aaaccaaaga
     1201 tgccgcagcg cagcgccttg ccaagcgcct cctttgcggc gaaatgggcc gccgcggtct
     1261 cggcggggcg gggctttgcg gcgatcagag cgttctcctg cgccgaaaac accctctcgc
     1321 aaaagcgtgg gttccccatg ctcttctcca agcggctcac cctcgccgcg tcaaagccaa
     1381 gttgcagcat cgcgtcctcc cttgtgtctg ttactccctg ccggcgggca cctcccgccg
     1441 gcccctgcaa aactctttgc gcgggcggga taaaggcccc gccgcattcg cccgcacgcc
     1501 tgccgctggg gccgcgcaga gtgcttcagg cgcaacggct ttttgcggcc cgccgcgcct
     1561 ttgccccggg cgggagggga catccccgcg gccctccatc ccctcgccgt ctcatcccgc
     1621 ttgcacctgc ggggagggca tcggccacag ggtacgcccc ctcgcgcact gcccgcagtg
     1681 gcgccgaagc gtttcaacac gcctccccct gcgcctttct cttttttatc cagtatacca
     1741 caaaacatcc ctttttgaac gccggtggtc cgcaaaaccg cttgcaaaag cccggcaaag
     1801 gtgctatcat gacaaaagat aactcgatga acggggaaag taggcttttc ctcccctctc
     1861 gcagagagcg gcgcattgct gggagcgccg cagagggaca aaggccgaag ttcccccggg
     1921 agaggcccgg ctgaacgcgc tccaagtagg ccgggacggt tcgcccccgt tacaggacgg
     1981 caaagtgccc tgccggagcg ccgctccgtc cgggaagctg ggtggtaccg cggagtgtca
     2041 cgcttcgccc ctgcatgtcc ttgcaggcgg cggggcgttt tttgttgccc tgcgcctttc
     2101 cagacgatca attttatttg aggtgagtac gaagatgaga gaagcactgg aaaaaatccg
     2161 ccaaagcgcg gaagaactgc tcgccaaggc ggacagcgaa gccgcgctgg aagaactgcg
     2221 ggtgcggttc ctcggcaaaa agggcgaggt gaccgcgatc ctcaagcaga tgggcagcct
     2281 ctccgcagag gagcgcccaa agataggcgc ccttgccaac gaggtgcggg ccgccatcga
     2341 cgccgaggtc cgccagcggg gcaaagaact ggcggccggg ctgctggagc agcgcctcgc
     2401 cgccgagcgc atcgacgtca ccctgccggg ccggcgcccg gtgcagggcc gtcggcaccc
     2461 gtttcagcag gtgctggacg agctcaagga gatctttttg ggcatgggct tcgaggtcaa
     2521 ggcgggcccg gaggtggagt acgaccacta caacttcgag atgctcaaca tgcccaaaag
     2581 ccacccggcc cgcgatacgc aggacacctt ttacatcacg gaaaacatcc tgctgcgcac
     2641 ccagaccagc ccgatgcaga tccgcaccat ggaggcgcag aagcccccta tccgcatcat
     2701 ctgcccgggc cgcgtctacc gcagcgacgc ggtggacgcc acccactccc cgctgtttca
     2761 ccagatcgag gggctggtgg tggacaagga catcactttc accgacctga aaggcacgct
     2821 ggaggcgttc atcaaaaagc tctacggcga agagacgcgg gtgcgctttc gcccccacca
     2881 cttcccctac accgagccct cggccgagat ggacatgagc tgcttcaaat gcggcggcaa
     2941 gggctgcagc ctgtgcaagg gcgagggctg gatcgagatc ctgggctgcg gcatggtgca
     3001 cccccaggtg ctggaaaact gcaacatcga ccccgaggtc tacagcggct ttgcctttgg
     3061 cgtgggcctt gagcgcatcg ccatgatgcg gtacggcatc gacgacatgc ggctgttcta
     3121 cgagaacgac ctgcgctttc tcaagcaatt ctgaccctga cattcacaca gccgtgtgct
     3181 gactttggag gttttagaga tgagattacc actgaactgg gtgcgtgact acgccgacat
     3241 cgtgaccacg ccccatgaat atgcggaaaa gatgaccatg agcggcagca aggtggaggg
     3301 atatacctgc gaggccgacg ggatcaaaaa cgtggtcatc ggcaaaattt tgaagctgga
     3361 gcgccacccc gactccgacc atctgtgggt ctgcacggtg gacgtgggcc aggaggcgcc
     3421 gctcaccatc gtgaccggcg cgcagaacct gaaagagggc gatctttgcc ccaccgccct
     3481 ggacggcagc accctgcccg gcggggtgaa gatccgccgc ggcaagctgc ggggtgtcga
     3541 gtcctgcggt atgctctgca gcctgggcga gctgggcctc accacccacg actttccctc
     3601 cgccgtcgag gatggcatca tggtgctgga cgaggaggtg ccggtgggca ccgacgccgt
     3661 caaagcgctg gggatggacg acgtctcggt ggagtttgag atcaccccga accgccccga
     3721 ctgcctgtcc atccgtggcc tggcccgcga gtcggccgcc acctacggcg tgccgttccg
     3781 cgaccacctg cccgccgcgc ccgccggcga gggggatatc cgcgcctggc tgagcgtgga
     3841 gatcaccgcc ccgggatgca gccgctacgt ggcggccatg gtcaccaacg tgcgggtcaa
     3901 gtccagcccc cgctggctgc gggagcggct gcgcctgtgc ggcgtgcgcc ccatcaacaa
     3961 catcgtggat atcaccaact atgtgatgct ggaatacggc cacccgatgc acgcctttga
     4021 ccacgcctat gtgggcggca agcacatcca tgtgcgcatg gcccgtgagg gtgaaaagat
     4081 cgtcaccctg gacggggtgg agcgcaatct ggacccgacc atgatggtca tcgccgacga
     4141 caaggtgccg atggcggtgg cgggcgtcat gggcggggag ttctccggca tctacgacaa
     4201 caccaagacc atcgtctttg agggcgcctg ctttgacggc ccctcggtac gggtcacggc
     4261 caaaaaggtc ggcctgcgca ccgaggcgtc cggccgcttt gaaaagggcc tcaaccctga
     4321 aaactgcctg cccgccgtcc gccgcgcgct ggagctggtg gccgagctgg acgcgggcga
     4381 cgtgatcggc ggcatcatcg acgtctatcc ccagccccgc gccgagcacc gcatccccct
     4441 gcgcccgggc gcggtcaacc gcctgctggg caccgacgtg ccggtggacg agatgatcgc
     4501 catcctgaag ccgctgggct tcggcatcga gaaaaagccc ctcaccgccc tggtggagca
     4561 cgaggagagc ccctatgtgg tcgtggtgcc g
//
LOCUS       DBMDYA010000146         4576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4553_length_4576_cov_1.658324, whole genome shotgun sequence.
ACCESSION   DBMDYA010000146 DBMDYA010000000
VERSION     DBMDYA010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4553_length_4576_cov_1.658324"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="ACZPFK_04855"
     CDS             <1..241
                     /locus_tag="ACZPFK_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR1405672.1"
                     /translation="TPMEETTPDQWQAQILARVMAKHPVVLVSDQPPGLVRRMGLIPA
                     ATLPEAVELADGILGRREQIVVIPDGVACIPGLKR"
     gene            295..1440
                     /locus_tag="ACZPFK_04860"
     CDS             295..1440
                     /locus_tag="ACZPFK_04860"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIR1405673.1"
                     /translation="MKIVIAADSFKGSLTSAGVVDAVRRGVGEELPGAQVVGVPVADG
                     GEGTVDAFHAAAGGTLREETVRGPLGRPVRARYGILPDGTAVIEMAQASGLTLLSQAE
                     LNPLCATTYGTGELVRAALDRGCRRFILGLGGSATNDGGAGFAEALGARFLDAEGAPL
                     SPGGGALAKLAAIDCSGLDPRLQGCEFWAASDVSNPLCGPYGASAVYGPQKGAGGEEI
                     ARLDAALANYARVLEAQLGRSIARQPSAGAAGGLGAALLAFCGAEIRSGIELLLDAAR
                     FEELLRDTALVVTGEGRIDGQSAYGKVPAGVARRTKMAGDIPVVAIVGCVGEGADALY
                     DLGVDAILPIAEGAMTLKESMQNADALVRKAAKRMIRLLRAGAKIKF"
     gene            1683..3476
                     /locus_tag="ACZPFK_04865"
     CDS             1683..3476
                     /locus_tag="ACZPFK_04865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405674.1"
                     /translation="MCVCIFSMVFQYLLATSFGAVRIHRVSMQTESGLTLSGDLYVPD
                     SATAEHPAPAVVLTHGSWKSREVMSTYTMELSRRGYVVFAFDQYGHGESGNTPSTGYD
                     TYDAFRFVCTLPYVDQEQIAATGHSSAGRALNKAFTLAEEAGGPQFAALIPIDFEPDY
                     KNADGNYYNKYGNVSYGVVASKYDDWFFSVKAEDGTWQNAPRDYIYTDNARSFLAFGK
                     EPSAFEGQPEWGKVYTQVIDGKETYRVIYQSDEIHSWSVVSPQSCAYAIDFLELTMPA
                     PNPIPSSDQHGLLKECISAVGMIGVLLFVVYSVYCLTESSLFSSLKADGPVVRQEAPK
                     TLAGHLWFWGGLVATALFSYITFFIICPPVYNLPAEELSPLLPQIQTLCFGLWGAVGG
                     LFSCLVMWMNYHFYGKKNGFDLNETGIVMSGRKWLSTIALSLIAVAGGFLVVFAADYF
                     FRMDFEFWVIAFKAFRAEKVGWMLAAAPLYLLYYIPNSIAVNCFRNNKVGGPEWVNVL
                     ILGLFNAASSIAIEAIQYGTFVATGIPFWSKTSFERQLGCWLYGIIIILVVAPFVARK
                     LYKKTRNPWLAGIINGLIVTVMSVAFTAQYY"
     gene            3666..4376
                     /locus_tag="ACZPFK_04870"
     CDS             3666..4376
                     /locus_tag="ACZPFK_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1405675.1"
                     /translation="MANEKILVVDDDRAIVEALTIQLKKEGFEVAAAYDGYGALDVLS
                     GGGIDLLLIDVMMPQLDGFSAIMRIREKQNLPILVMSAKTEDTDKILGLSIGADDYIT
                     KPFNAAEVVARVKSHLRRYYQLGGAGPAASPDSVAQVGRLSYDFDAHELSADGAPVRL
                     TATETRIVELLFRNPGRIFSAEEIYSRVWQEESYACENTVMVHIRHIREKVELNPKEP
                     EYIKVVWGLGYKLEKHER"
     gene            4366..>4576
                     /locus_tag="ACZPFK_04875"
     CDS             4366..>4576
                     /locus_tag="ACZPFK_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405676.1"
                     /translation="MNGKMEAHPQEKRAGGLPLLFSLFLGLALLAHAAVLMVFGGAAT
                     GLADWEFVRGDLSRHPDYAAAVANLY"
BASE COUNT          845 a         1331 c         1498 g          902 t
ORIGIN      
        1 cacaccgatg gaagagacaa cgcccgacca gtggcaggcc cagatactgg cccgggtgat
       61 ggcgaaacat ccagtcgtgc tggtcagtga ccagccgccg ggattggtgc ggcgcatggg
      121 gctgatcccc gccgccactc tgcctgaagc tgtggaactg gcggacggta tcctgggccg
      181 tcgggagcag attgtcgtca tcccggatgg ggtggcctgt atcccgggtc tgaagcgctg
      241 aaagaaccca agggtcatct gcgcaaatct gaactctcag ggagagccaa gcttatgaaa
      301 atcgtaattg ctgccgattc ttttaaaggc agcctcacct ccgccggcgt ggtggacgcc
      361 gtgcggcggg gggtggggga ggaactgccg ggcgcgcagg tcgtgggcgt cccggttgcg
      421 gacggcgggg agggcacggt ggacgctttt cacgccgccg cgggaggaac gctccgcgaa
      481 gagactgtcc gcgggccgct gggccgcccc gtccgtgccc ggtatggtat tctgccggac
      541 ggcacagcgg tgatcgaaat ggcccaggcc agcggtttga ccctactcag ccaggcggag
      601 ctaaacccgc tttgtgccac cacctatggt accggcgagc tggtgcgcgc cgccctggac
      661 cggggctgcc gcagattcat cctcggcttg ggcggcagtg ccaccaacga cggtggcgcg
      721 gggttcgcgg aggctttggg ggcgcggttc ctcgacgccg agggggcgcc cctctcccca
      781 ggcggcggtg ccttggcaaa gctggcggcc atcgattgtt ccgggctgga tccgcgtctg
      841 caggggtgcg agttctgggc ggcgagcgac gtctcaaacc ccctttgcgg gccttacgga
      901 gcctctgcgg tgtatggccc gcaaaaaggc gcgggcggcg aagagattgc aagactggac
      961 gcggcgctgg caaattatgc ccgcgtgctg gaggcacagt tggggcgcag tattgcccgg
     1021 cagccgtccg cgggtgcggc cggtggcctg ggagcggcgc tgctcgcgtt ctgcggggca
     1081 gagatccgct cggggattga gctgctgctg gatgcagccc gctttgaaga gctcctgcgg
     1141 gacaccgctc tggtggtcac cggcgagggg cgcatcgacg gacagtccgc ctatggcaag
     1201 gtgccggcag gtgttgcgcg ccggacgaaa atggcgggcg acatcccggt ggtggccatc
     1261 gtcggctgtg tgggggaggg ggccgacgcg ctgtacgacc tcggggtcga tgcgatcctg
     1321 cccattgccg aaggggcgat gacattgaag gagtccatgc aaaatgcgga cgccctggtc
     1381 cgcaaggcgg cgaaacgaat gatccgtctg ctgagggcgg gggcaaaaat caaattttag
     1441 atgcatgggg cggtttcgcc ctccagcgga aaagcactgg caagcgcgcg gccgcgcgga
     1501 gagaagcggc ggcgcggccc tgggtattac ctccgccggc accggcccca aaaggggtgc
     1561 cttttgtgtg acgctgagat agaagaagat caaaggagga ttttaaattg gaaagcaata
     1621 aggtaaccgt tctggctcca gacagcggga agacgcgcgc taaaatcggg atgctggtag
     1681 cagtgtgtgt ctgcattttc agcatggtgt tccagtatct gctggccacc agcttcggcg
     1741 cggtccggat ccaccgggtc agtatgcaga cggagagtgg gctcaccctg tcgggtgacc
     1801 tctatgtgcc ggacagcgct acggcggagc atcccgcccc ggccgtcgtt ttgacccatg
     1861 gctcatggaa gtcccgtgag gtcatgtcca cctataccat ggagctgtcg cggcggggct
     1921 acgtggtctt tgcatttgat cagtacggcc acggtgaatc ggggaatacc ccctccaccg
     1981 gctatgatac ctacgacgcg ttccggttcg tttgcacgct gccctatgtg gatcaggagc
     2041 aaatcgccgc cactgggcac tcctctgcag ggcgcgcgct gaacaaggcg ttcaccttgg
     2101 ccgaagaggc gggcgggccg cagttcgcgg ctctgatccc cattgacttc gagccggatt
     2161 acaagaatgc cgacggcaac tattacaaca aatacggcaa cgtgagttat ggcgtggtcg
     2221 cctccaagta tgacgactgg ttcttctcgg tcaaggccga ggacggcacc tggcagaacg
     2281 cgcctcgcga ctatatctat accgataacg ccaggtcttt cctggctttc ggcaaagagc
     2341 cctccgcgtt tgagggacag cctgagtggg gcaaggtcta tacccaagtg atcgacggca
     2401 aggagaccta ccgcgtcatc tatcagtctg acgagatcca ttcctggtcg gtggtctccc
     2461 ctcagtcctg cgcttacgcc atcgactttt tagagctcac catgcccgcg cccaacccca
     2521 tcccctcctc tgaccagcac gggctgctga aagagtgcat ctccgccgtc ggcatgatcg
     2581 gcgtgcttct ctttgtggtt tattcggtct actgcctcac cgagagcagc ctgttcagct
     2641 cgctgaaagc cgacggcccg gtggttcgac aggaggcccc caagacgctg gcggggcacc
     2701 tgtggttctg gggcggcctg gtggccactg cactcttctc ctacattacc tttttcatca
     2761 tctgcccccc ggtctacaat ctgcccgcag aggagctttc gcccttgctt ccccagatcc
     2821 agaccctctg cttcggcctg tggggcgcag tgggcggact tttctcctgc ctggtcatgt
     2881 ggatgaacta tcacttctac ggcaaaaaga acgggtttga cctgaacgag accggcatcg
     2941 tgatgagcgg gcgcaagtgg ctctccacca tcgcattgtc tctgatcgcc gtggcgggcg
     3001 gctttctggt ggtatttgcg gccgattact tcttccgcat ggatttcgag ttctgggtga
     3061 tcgctttcaa ggctttccgc gccgaaaagg tgggctggat gctggcggcg gcgccgcttt
     3121 acctgctcta ttacatcccc aattccatcg ccgtcaactg cttccgcaat aacaaggtgg
     3181 gcgggccgga atgggtcaac gtgctgatcc tgggcctctt caacgcggcc agctccatcg
     3241 ccatagaggc catccagtac ggcacctttg tcgccacggg tattcctttc tggtccaaaa
     3301 ccagctttga gcggcagttg ggctgctggc tgtatggcat catcatcatc ctggtggtag
     3361 cgcctttcgt ggcccgcaag ctttacaaaa agacccgcaa tccctggctg gccggcatca
     3421 tcaacggcct gatcgtgacc gtcatgtcgg tcgcctttac cgcccagtat tattgaactg
     3481 aggggcggga aaaaagccgg ttgccccgcg ctgcatgata cttaagaaaa atttaaatat
     3541 tttgcccggc cttttttaag gccgggcttt tataatggga aaagcggaaa ggtacaggcg
     3601 gaaccttttg cggaaatgtg gtatgctaga ggtgtacgaa aaaggtgtga agtgagggaa
     3661 accgcatggc gaatgagaag atattggtgg tggacgacga cagggccatc gtggaggcgc
     3721 tgacgatcca gctgaaaaaa gagggctttg aggtggcggc ggcctatgac ggctacggcg
     3781 cgctggacgt gctctcgggc ggcggcatcg acctgctgct catcgacgtg atgatgccgc
     3841 agctggacgg cttttcggcg atcatgcgca tccgggaaaa gcagaacctg cccatcctgg
     3901 tgatgagcgc caaaacggag gacaccgaca agatcctcgg cctctccatc ggggcggacg
     3961 actatatcac caagccgttc aatgcggcgg aggtggtggc ccgggtgaaa agccatctgc
     4021 ggcgctacta tcagctgggc ggggcgggcc ccgcggcctc ccccgacagc gtggcccagg
     4081 tggggcggct gagctacgat tttgacgccc acgagctcag cgccgacggc gcgccggtgc
     4141 ggctgaccgc caccgagacc cgcatcgtgg agctgctgtt ccgcaacccg ggccgcatct
     4201 tctcggcgga ggagatctat tcccgcgtct ggcaggagga atcgtatgcc tgtgagaaca
     4261 ccgtcatggt gcacatccgt catatccgcg aaaaggtcga gctcaaccca aaagaacccg
     4321 aatatataaa ggtggtgtgg ggacttggat acaaattgga aaaacatgaa cggtaagatg
     4381 gaggcgcacc cgcaggaaaa acgcgccggc ggtctgccgc tgcttttttc tctctttctc
     4441 ggcctggcgc tgctggccca cgcggcggtg ctgatggtct ttggcggggc ggcgaccggc
     4501 cttgccgact gggagtttgt caggggcgac ctgagccgtc atcccgatta tgcggccgcg
     4561 gtggcgaacc tctacc
//
LOCUS       DBMDYA010000147         4572 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4556_length_4572_cov_1.331246, whole genome shotgun sequence.
ACCESSION   DBMDYA010000147 DBMDYA010000000
VERSION     DBMDYA010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4556_length_4572_cov_1.331246"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="ACZPFK_04880"
     CDS             complement(<1..311)
                     /locus_tag="ACZPFK_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1405677.1"
                     /translation="MANSKSLLPGSAGMLLLQLLSEGDLYGYQMTEELRRRSDDTFVM
                     KAGTLYPILHGLEQEGKVESYERQGEGGAPGKPRRYYHITRAGRAALRQQRSEWDRLS
                     GA"
     gene            complement(1169..2956)
                     /locus_tag="ACZPFK_04885"
     CDS             complement(1169..2956)
                     /locus_tag="ACZPFK_04885"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR1405678.1"
                     /translation="MSSLTSNQHVLDWVKEMEDLMTPDKTIWIDGSEGQLRALREEAF
                     KTGELTELNQEKLPGCVLHHTAKNDVARVEDRTFICTSNKADDCAMINWMEPTAMKAK
                     LTPLYQGVMKGRTMYVIPYCMGPVGSQFSKVGIEVTDSIYVVLNMDIMTRIGIKALRQ
                     LGDSNDFVRGQHARADIDAENRYIVHFPEDNAIWSINSGYGGNVLLGKKCFALRIACY
                     QGYKEGWMAEHMLILGVEDPSGHVDYVTAAFPSACGKTNLAMLIPPEVYAKKGYKVWT
                     VGDDIAWMHVGADGRLYAINPENGFFGVAPGTNEKSNPNALATTRQGTIFTNVAHNLT
                     DNTVWWEGLDKNPPEDAQEWTGLAVDGKEYVANGGKLAHPNSRFTAPATGCPCLSKEF
                     NNPDGVPVTAMVFGGRRAKTAPLVYQSFSWNHGVYVGSSMASETTAAAAGAVGVVRRD
                     PMAMRPFFGYNVGDYFAHWLDMGKKLGDKAPKIFHVNWFRTDDEGHFIWPGFGDNMRV
                     LMWILKRCKGEVDAVETPIGYLPHAEDIDITGLDGVTLDTVKNLLNVDKDLWLEDAKG
                     VEELYDQIGDHVPAELRAELNALKDRLSK"
     gene            complement(3848..4483)
                     /locus_tag="ACZPFK_04890"
     CDS             complement(3848..4483)
                     /locus_tag="ACZPFK_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405679.1"
                     /translation="MKNNGIRTQGHRPLDGPKLCQAVLLGAYGAALLICLVCDLATGG
                     GLSWFYIVLCSLLLAFSLLNLPFLLKRHRLVFPALAATLSLFLLLAACEAVTGGGWLR
                     PYGVPIAAFPLTFAWAMLLVTQLKRLNWFFRSALLSLLAGVLALTMDAWVGAVLAGET
                     DHFAQVLAAGLGRAGSLGGILAAACFFLYFAVGAVLGVLSAAKRARGEEKR"
BASE COUNT          811 a         1463 c         1433 g          865 t
ORIGIN      
        1 gcccccgaga ggcgatccca ttcgctgcgc tgctggcgca gggccgcccg cccggcgcgg
       61 gtgatgtgat aatagcggcg cggcttgccc ggcgcgccgc cctcgccctg ccgctcatag
      121 ctctccacct tgccctcctg ctccagcccg tgcaggatcg ggtacagggt gcccgctttc
      181 atcacgaacg tgtcgtcaga gcggcggcgc agctcctccg tcatctgata gccgtataga
      241 tccccctcgc tcagcagctg cagcagcagc atacccgcag agccgggcag caagctcttg
      301 ctgttcgcca tctttccatc cctccgtttt atatatagat attctatatt tactgttccc
      361 cattatatat cgaatatcta tatatgtcaa ggcggggaag atcatttttt cgatcctctt
      421 ttctcaaggc ccgcgcgggg ccgcgcccgt cctgcgcgct cttgatcctt ttgtccgctt
      481 cctcccgccc ggcgcacagg gcccagtaaa ctcgcgccac ccggaaaacc cgcatgatcc
      541 ggtaagtccg cgccactccg taaggcgctg cctggggctt ggcagtatgc ggatttcgac
      601 acaaaccgga ggtgtccggc ggacggccat cgccgtgtac ggcgcacggg gttcggcaca
      661 ccgcatctct cggcaggccc gctcgcgcgc cactcggcaa gttcgcccca cttggcaagt
      721 tcccaccact ggacaaaccg gaacggcccg gcaagtccgc actgtctgac aaatcggcac
      781 cggctgacaa ggcgtcatct gagattttgg aaacatgcgg ctttcaaggc gtctgggggg
      841 caggcaaggc gccgccgtgt tccatgtgct gcgtccggcg agcgggccgc agcagcagcg
      901 cacggtgcga ggacccgtct cccagcgccc caccccgcac cgccctttgc cggcggccct
      961 gccgcccctg cataccgccg cccgggcgca ctggcgcgtc tccccgtcaa cggcctctgt
     1021 gcgccgcccc tcactcccgc cgcccgcggc gcccggcgcg ggcggcccgc agagggcgca
     1081 tccgcgaaaa gcgcaaaaaa caaaaaagcg gactgccgcg tttttcgcgc ggcagtccgc
     1141 aatatttata gaaagagaaa ggtgtgtttt atttggagag acggtctttg agtgcgttca
     1201 gctcagcgcg cagctcagcg ggcacgtggt cgccgatctg gtcatagagc tcttcgacgc
     1261 ccttggcgtc ctcgagccac agatccttgt cgacgttcag caggttcttg acagtgtcca
     1321 gggtgacgcc gtccagaccg gtgatgtcga tgtcctcggc gtggggcaga tagccgatcg
     1381 gggtctcgac cgcgtcgacc tcgcccttgc agcgcttgag gatccacatc agcacacgca
     1441 tgttgtcgcc gaagcccggc cagatgaagt gaccctcgtc gtcggtgcgg aaccagttga
     1501 cgtggaagat cttcggggcc ttgtcgccca gcttcttgcc catatccagc cagtgcgcga
     1561 aataatcgcc cacattgtag ccgaagaacg gacgcatggc catcgggtcg cggcggacca
     1621 cgcccacggc gccggcggcg gcggcggtgg tctcagaggc catggaggag cccacataca
     1681 cgccgtggtt ccagctgaag ctctggtaca ccagcggggc ggtcttggcg cggcggccgc
     1741 cgaagaccat cgcggtcacg ggcacgccgt cggggttgtt gaactctttg gacaggcagg
     1801 ggcagccggt ggccggggcg gtgaagcggc tgttcggatg agccagcttg ccgccgttcg
     1861 ccacatactc cttgccgtcg accgcgaggc cggtccactc ctgggcgtcc tcgggcgggt
     1921 tcttgtccag gccctcccac cagacggtgt tgtcggtcag gttgtgggcg acgttggtga
     1981 agatggtgcc ctgccgggtg gtggccagcg cgttggggtt ggatttctca ttggtgccgg
     2041 gcgcgacgcc gaagaagccg ttctccgggt tgatcgcgta caggcggccg tccgcaccca
     2101 cgtgcatcca ggcgatgtcg tcgccgacgg tccagacctt ataacccttt ttggcgtaga
     2161 cctcgggcgg gatcagcatg gcgagattgg tcttgccaca ggcggagggg aacgcggcgg
     2221 tgacgtaatc cacgtggccg ctgggatcct ccacgcccag gatcagcatg tgctcggcca
     2281 tccagccctc tttatagccc tggtagcagg cgatgcgcag cgcaaagcac tttttgccca
     2341 acagcacgtt gccgccgtag ccggagttga tggaccagat ggcgttgtcc tcggggaagt
     2401 ggacgatgta gcggttctcg gcgtcgatgt cggcgcgggc gtgctggccg cggacgaaat
     2461 cattcgagtc gcccagctgg cgcagggcct tgatgccgat gcgggtcatg atatccatgt
     2521 tcagcacgac gtagatggag tcggtgacct cgatgcccac cttggagaac tggctgccga
     2581 cgggacccat gcagtaggga atgacataca tggtgcggcc tttcatgacg ccctggtaca
     2641 gcggggtcag cttggctttc atcgccgtgg gctccatcca attgatcatg gcgcagtcgt
     2701 cggccttgtt ggaggtgcag ataaaagtgc ggtcctcaac acgggcgacg tcgttcttcg
     2761 cggtgtgatg cagcacgcag ccggggagct tctcctggtt cagctcggtc agctcgccgg
     2821 tcttgaaagc ctcttcgcgc agggcgcgca gctggccctc agagccgtcg atccagatgg
     2881 tcttgtccgg ggtcatgagg tcttccatct ctttgaccca gtccagcaca tgctggttgg
     2941 aggttaaact actcataaga cataactcac ctttctctat aaaagtaacg gatatctctg
     3001 tgattttgga ctcgcttcca acaaaatccg aaatcaaatt tattgtagca cagcgttaaa
     3061 tatttagcaa tatacaaagc atccaaaaac ccacgatttt gctatgacaa acgccgcttt
     3121 tcaaatcttt gcacaaactt aacgcatcct ctatccgggc tgggcgcggc ctgccgacgg
     3181 cacgccgcaa aagcgttttt tgcgtcctca ccccgccgca gccgcggctc gcccgcaggg
     3241 aagaaagcgc cgtgcgcggg ccgcgccaga ggggaaggct tgatcgtgcc gcagcgcacc
     3301 cgcaaagggg aaaacgccgg gcgggaagca cgagctttcg gctctgtgcc tctcggggcg
     3361 acctgcgccc cagggcttgc gccgggcggg aggtgcttgc ctttactctg ggcctttggg
     3421 gacggcctgc gccttatagc ttgggactgg cgcaaagtgc gtgtctttgc tcagtgcctt
     3481 ttggggcagc ccgcggcggg gcctggggcg ggcggaagat gcaagctttc ggctttgtgc
     3541 ctttcggggg gcctgtgccc tggggcttga gacgggcgta aagtgcgcgt ctttgctcag
     3601 tgccttttta gggggcggcc cgtgcctcgg agctgagggc tgacgcaaag tgcgcgcgct
     3661 ttcaactttg tgccttttgg ggcggtccgc ggcgggttcc accccgctcc cgaaaaagag
     3721 aacccgcggc ggacgccgcg ggttctcttg tatgaaaaag ccaccggctg caccggtgga
     3781 tacttattag ggcaacgatc ccgccgggaa agcggatccc cgggcctccc ccagccgggc
     3841 ggccgaccta ccgcttttct tcgccccggg cgcgctttgc cgcggagagg acacccaaca
     3901 ccgcgcccac tgcgaagtag agaaaaaagc aggcggcggc gaggatgccg cccaggctgc
     3961 cggcccggcc gagcccggca gccaggacct gcgcaaaatg gtccgtctcg cccgcgagca
     4021 ccgcccccac ccaggcgtcc atggtgaggg cgagcacacc cgccagcagc gagagcagcg
     4081 cggagcgaaa aaaccagttc agccgcttga gctgcgtcac cagcagcatc gcccaggcga
     4141 acgtcagcgg gaacgccgcg atgggcactc cataggggcg cagccatccg ccgcccgtga
     4201 cggcctcaca ggcggccagc agcagaaaaa gggacagcgt agccgcaagg gcgggaaaca
     4261 ctagccggtg ccgtttcaat aaaaacggga ggttcagcag ggagaaagcc agcagcagcg
     4321 agcagagtac gatataaaac caggagagcc cgccgcccgt cgccagatcg cagacaaggc
     4381 agatcagcag cgccgcgccg taagcgccca gcagaacagc ctggcagagc ttcggcccgt
     4441 cgaggggcct gtgcccctgt gttctgatcc cgttgttttt catgttttct ccccgcgcgg
     4501 gcataaagcc gaccgccgcg ccctctttat tttgatactg cctctacatc acttttacat
     4561 ttttgctgcc tg
//
LOCUS       DBMDYA010000148         4570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4558_length_4570_cov_1.850750, whole genome shotgun sequence.
ACCESSION   DBMDYA010000148 DBMDYA010000000
VERSION     DBMDYA010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_4570_cov_1.850750"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="ACZPFK_04895"
     rRNA            complement(<1..423)
                     /locus_tag="ACZPFK_04895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            998..1777
                     /locus_tag="ACZPFK_04900"
     CDS             998..1777
                     /locus_tag="ACZPFK_04900"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1405680.1"
                     /translation="MFLTINIGNANILAAAYEGREKLFCSQLRTDPLKTADEYAIGLK
                     NLLQLYGFSPEQLEGAILSCVAPALAGTLRQAVRLLREVRVLTVGPGLKTGLNIRIDN
                     LAELGADIVATAVAALERYPMPCLLVNFDTAITVMGLDAQRSLIGGCISPGVRLSLEA
                     LYTHAAQLPQIDLTRRPGSVAGASTVASMEAGLILGTASMVDGLIDRFAELFGCPPAV
                     VATGELCRSITSHCVHRIQVDGDLLTDGLALIYLRNCRAAS"
     gene            1968..2567
                     /locus_tag="ACZPFK_04905"
     CDS             1968..2567
                     /locus_tag="ACZPFK_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1405681.1"
                     /translation="MKNRTNTLRMTQLALLIAIEVVMALTPLGYLMIPPISATLMHIP
                     VIVGAVLLGPGCGAILGGVFGLTALWKASTAATSPVDMAFSPFLSGKPLASLVMCLLP
                     RILLGIIAALLFKAFLKLFKGKEGLSIGVAAVLSTICHTVMVLGCLALFFREFGMGVM
                     SVVLSLVTVSSAAEMLIAAVVSIALCVPLRAYLKRSSHS"
     gene            2757..3380
                     /gene="udk"
                     /locus_tag="ACZPFK_04910"
     CDS             2757..3380
                     /gene="udk"
                     /locus_tag="ACZPFK_04910"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR1405682.1"
                     /translation="METMVIGIAGGTGSGKTTLTDRLKRHFGDDVSVVYHDNYYKSHD
                     DLTYEQRCELNYDHPDAFDTDLLIEHLAALRAGQEIDCPVYDFTVHNRSDKTLRIKPS
                     RVLLIEGILIFQNRALRDLMDIKIFVDTDADVRLLRRLSRDVKERGRSLDSVVNQYLS
                     TVKPMHEQFVEPSKRAADIIVLEGGHNAVALDMIIQRVERHLQSAEK"
     gene            complement(3858..>4570)
                     /gene="pgmB"
                     /locus_tag="ACZPFK_04915"
     CDS             complement(3858..>4570)
                     /gene="pgmB"
                     /locus_tag="ACZPFK_04915"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIR1405683.1"
                     /translation="RHFCLNATPSRERMKKMHRGLLFDLDGVLVNTAKYHYLAWSEIA
                     QQLGIPFTRRDNERLKGVSRERSLEILLELGKREMSPEERAELCAQKNERYLSYIRRM
                     TRAEILPGVCGFLQCARAEGYQIALGSASRNSRLILERLELLDSFDAIVDGTGVHRAK
                     PDPEVFLRGAALLGLGAAECIVFEDAPAGIRAAHAGGMAAVGIGRPCDLPEADLCLPG
                     LEGIGIAEVERRLERSAT"
BASE COUNT          806 a         1470 c         1278 g         1016 t
ORIGIN      
        1 ctcacgcggc gtcgctgcat cagagtttcc tccattgtgc aatatccccc actgctgcct
       61 cccgtaggag tctgggccgt gtctcagtcc caatgtggcc gatcaacctc tcagtccggc
      121 taccgatcgt cgccatggtg ggccgttacc tcaccatcta gctaatcgga cgcgagtcca
      181 tctttcagcg aataaatcct ttgatcactg caccatgcgg tgctgtggtc ttatgcggta
      241 ttagcagtcg tttccaactg ttgtccccca ctgaaaggta ggttcctcac gtgttactca
      301 cccgttcgcc actaagttaa atcgcttcca tccgaaaact tccgccatct aactccgttc
      361 gacttgcatg tgttaggcgc gccgccagcg ttcgtcctga gccaggatca aactcttaat
      421 caatgatatt aaaatcagtt gacctgaatt taatcgcttt tcactcagca cgcaatcgct
      481 tgcgtccttg ttgtgaatta ctttaaagga atgtttttta gtttaacgag caggctcatc
      541 ccctgctcta agggttcctt caagctttcc tatattgttt aattttcaag gtcctgtccc
      601 ccaaaacttt ggaggttttg ctgtgctcac ttccgtggga cagcttattt attatagcgt
      661 actcggtttt gtttgtcaag cactttttgg aatttttttg aattcttttt gtgcccggcc
      721 gattggcctt tccgctatgg tttgcggccc cgcttggggc agctcgatta tgataccaca
      781 ctcctgccgg gaaagtcaac accttttttc aaaaaatttt aaggcttttt tcctttgccc
      841 ccatcccctc cctctcggcc cggtttcagc ccccttggac ggagaccttt ctcttccttt
      901 ataatgtaat atatatagaa aggcgctttt tctctttgtt tttcttgcct tttgccgtct
      961 gttgcggtag gataaggggc ggaaaggggg aaccgccttg tttctcacca tcaatatcgg
     1021 caacgccaat atcctggccg cagcctacga ggggcgggag aagctctttt gctcccagct
     1081 gcgcaccgac ccgctgaaga ctgcggacga gtacgccatc ggcctgaaga acctgcttca
     1141 gctctacggc ttttcccccg agcagctgga gggcgcgatc ctcagctgtg tggcgcccgc
     1201 cctggccggc acgctgcgcc aggccgtgcg cctgcttcgg gaggtgcggg tgctcacggt
     1261 ggggcccggc ctcaagaccg gccttaacat ccgcatcgac aacctcgccg agctgggcgc
     1321 cgatatcgtg gccactgcgg tcgcggcgct ggagcgctat cccatgccct gcctgctggt
     1381 gaacttcgac accgctatca cggtcatggg gctggacgcg cagcgcagcc tgatcggcgg
     1441 ctgcatctcg cccggcgtcc gcctctccct tgaggcgctt tatacccatg ccgcccagct
     1501 gcctcagatc gacctgaccc gccggcccgg cagtgtggcg ggggccagca cggtggccag
     1561 catggaggcg ggcctgatct tgggcaccgc gtcgatggtg gacggtctga tcgaccgctt
     1621 tgcagagctg ttcggctgcc cgcccgccgt cgtcgccacc ggcgagctct gccgctccat
     1681 caccagccac tgcgtccacc gcatccaggt ggacggggac ctgctcaccg acggactggc
     1741 gctgatctat ctgcgcaatt gccgcgccgc ctcttaagcc gggcgggccg cctcgtcggc
     1801 tccatgggcc acgggccggc tgcgtggggg ctgcggccca ggcagctcct tttcattttc
     1861 ggtgcacccg tgccgcgggg gagcggtccc ctgccggcgc gtttgcataa atttgattgc
     1921 gctgtatccg tcgacagggc ggaacggcag caaggaggaa ttctcacatg aaaaaccgca
     1981 ccaacaccct gcgcatgacc caattggcgc tgctcatcgc catcgaggtg gtgatggcgc
     2041 tgactccgct gggctatctg atgatcccgc ccatctcggc gactctgatg cacatcccgg
     2101 tcatcgtggg ggccgtgctt ctggggccgg gctgcggggc gatcctgggg ggcgtattcg
     2161 gcctgacggc gctctggaaa gcttccaccg ccgccaccag ccccgtggac atggcgttca
     2221 gcccctttct cagcggcaag ccgctggcca gcctggtgat gtgcctgctg ccccgcatcc
     2281 tgctggggat catcgccgcc ctgctcttca aggcttttct caagcttttt aagggcaagg
     2341 aggggctttc catcggcgtc gcggccgtgc tgtccaccat ctgccacacc gtgatggtgc
     2401 tgggctgcct ggcactgttc ttccgcgaat tcggcatggg tgtgatgagc gtagtgctct
     2461 cactggtcac cgtctccagc gccgccgaaa tgctgattgc cgcggtggtc tccatcgccc
     2521 tctgtgtgcc gctgcgggcc tatctcaagc gcagctccca cagctgaccg atccaccccg
     2581 acggggcctc gcggcgaggt cccgtttttt ctttttcgcc attttccgcc ttttccttta
     2641 ccgcgccctc aatttatatt atactggtct ttagacggat ttccgctctg cggccctgcc
     2701 gtgaaagggg gcccgggcgc aaaactttgg tgtgcaggga aaggatgatc taaaagatgg
     2761 agacgatggt gatcggcatc gcgggcggta ccggcagcgg caagacgacc ctgaccgacc
     2821 ggctgaagcg gcatttcggg gacgacgtga gcgtggtcta tcacgacaac tactacaaga
     2881 gccacgacga cctgacctat gagcagcgct gcgagctgaa ctatgaccat ccggacgcct
     2941 ttgacaccga cctgctcatc gagcacctgg cggcgctgcg cgccgggcag gaaatcgact
     3001 gcccggtcta cgactttacg gtgcacaacc gctctgacaa gactctgcgc atcaagccct
     3061 cgcgggtgct gctcatcgag ggcatcctga tctttcagaa ccgggcgctg cgggacctca
     3121 tggacatcaa gatctttgtg gacaccgacg ctgatgtgcg gctgctgcgc cgcctctccc
     3181 gcgatgtaaa agagcgcggg cgcagcctgg actcggtggt gaaccaatac ctgtccaccg
     3241 tcaagcccat gcacgagcag tttgtggagc cgtccaagcg cgccgcggac atcattgtgc
     3301 tcgagggcgg gcacaacgcc gtggcgctgg acatgatcat ccagcgggtg gaacggcatc
     3361 tgcagagcgc tgaaaaataa actttgcgga caaaagcggc ggggcgcttg ggcgcgtcga
     3421 agcaaaaaaa tagtggtttt ggcccggcgg ctccggccct cggcagattc aaaaaggttg
     3481 gcaatccgca aagattgcca accttttttt cacaccgggt tttctaaaaa atcgtcgggc
     3541 ctcacccgcc gcgcacccgg ctgcggcccc ctgcggccgg acgcaaaaga acgggctttt
     3601 tatggccgtg tcccacggac aggccacatg gtccctggcc ttgaaatttt gggcacccgc
     3661 ccacggagtg ggcggaggcg cgtaaaaagc aacttatttc tagggtcgtg acccggggac
     3721 ggcccacatg gccaaaaaat agcctccgct ttgctgcggc tgttttttta ttactccgca
     3781 ataaaaaaca acttatcttg atggccgcgg cccgaggacg ggccacatgg ccgtaaaaaa
     3841 tagcctgcgg ctatttttta tgtcgcgctt cgctccagcc gcctctcgac ctccgcgatg
     3901 ccgatcccct ccagacccgg caggcagaga tccgcctcgg gcaggtcgca gggcctgccg
     3961 atgcccaccg ccgccatgcc gccggcatgg gccgcacgga tgccggcggg cgcgtcctca
     4021 aagacgatgc actcggccgc gcccagcccc agcagcgccg ctcctctcaa aaagacttca
     4081 gggtccggtt tggcgcggtg caccccggtg ccgtccacga tcgcgtcaaa gctgtccagc
     4141 agctccaggc gctccaggat cagacggctg ttcctgctcg cgctgcccag ggcgatttgg
     4201 tagccctctg cccgcgcgca ctgcagaaaa ccgcacacgc cgggcaaaat ctccgcgcgg
     4261 gtcatccgcc ggatatagga gagatagcgc tcgtttttct gcgcgcagag ctccgccctc
     4321 tcctccgggc tcatctctct cttacccagc tccagcagga tctcgaggct gcgctcccgg
     4381 ctcacccctt tcagccgttc attgtcccgc cgggtaaacg ggatccccag ctgctgcgcg
     4441 atctcactcc aggccaaata atggtacttc gccgtgttta ctagtacccc gtccagatcg
     4501 aaaagcaaac cccgatgcat ctttttcatc ctctcccttg agggcgtggc gttcaagcaa
     4561 aagtgccgca
//
LOCUS       DBMDYA010000149         4568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4560_length_4568_cov_1.460936, whole genome shotgun sequence.
ACCESSION   DBMDYA010000149 DBMDYA010000000
VERSION     DBMDYA010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4560_length_4568_cov_1.460936"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="ACZPFK_04920"
     CDS             <1..366
                     /locus_tag="ACZPFK_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405684.1"
                     /translation="LRLVGLVNKADSYPAMLSGGQQQRVAIARALAIDPQIMLFDEPT
                     SALDPELTAEVTAVIAKLAQQDITMLIVTHEMEFARTVADRILFFEDGEIIAGGTAKE
                     FFESQQNDRIKRFLNGFQK"
     gene            complement(550..1125)
                     /locus_tag="ACZPFK_04925"
     CDS             complement(550..1125)
                     /locus_tag="ACZPFK_04925"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1405685.1"
                     /translation="MELLEQRILKDGKVKEGNILKVDSFLNHQIDIALMNEMGREFFR
                     LFRGETITKIVTIEASGIAIAAIAAQYFHVPIVFAKKAKSKNIDGDVYTSVIRSYTHG
                     NDYTAMLEKKFLTPQDRILILDDFLATGKAQLGLLDIARQAGATVAGIGIAVEKGFQG
                     GGDKLREAGYNLQSLAIIDEMSPEGIVFRHV"
     gene            complement(1397..2848)
                     /locus_tag="ACZPFK_04930"
     CDS             complement(1397..2848)
                     /locus_tag="ACZPFK_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1405686.1"
                     /translation="MNGRIFDTRVQWLKYKVLKEVAKHAYADTLQDAYRDVPKAIIPD
                     GAKATMRCCIYKERAVLGERVHSALGGDPENPNVIQVLDIACDECPVSGYTVGPDCRG
                     CIAHRCEEVCPRGAITIQDQHAIIDHSKCVGCGKCASVCPYNAISKHTRPCENACKVN
                     AIHMNEEKKAEIDNSKCISCGSCVYMCPFGAIVDRSFIVDVVKLLRASKNNQNYKVYA
                     VVAPSVSSQFNYAKLGQVISGLKVLGFYNVIEAALGADMVSLAESRELAQRGFLTSSC
                     CPAFVDYIKKNFPAMADKISSNLSPMGAVGKFVKATDPTAKVVFIGPCTAKKMEFQLP
                     EVSPYVDSVLTFEELHALFEACEIDLTALEEDVLDNASYYGRIFARSGGLSDAVAEAF
                     REQDIKDFEAKPVVCNGIEECKLALLKASRNLLPGNFIEGMACINGCIGGAGCLTHGP
                     KDKNEVDEYGRLALEKTIESAAGIAFGVSPEKE"
     gene            complement(2922..4094)
                     /locus_tag="ACZPFK_04935"
     CDS             complement(2922..4094)
                     /locus_tag="ACZPFK_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1405687.1"
                     /translation="MNNLWTDTGYVSINKTGEQLCGDKVEIIGTADDCLTLVLADGLG
                     SGVKANILSTLTSKILSTMIDAGMSIEDCVDTIVKTLPVCQVRKVAYSTFTIIHIEHN
                     DTARIIQFDNPNVIFLRDGKSVDYPIVSRVIGDKTVLESTISLQLDDTFIAMSDGAIY
                     AGVGKTLNFGWQRENIVDFAEAIFDWSMSAKTISTMIVDECNSLYEGEPGDDTTIACV
                     KIKKRNPVNLMIGPPADPKDVGMMMNLFFSKEGAKIVCGGTTSTLAAQFLGKPINTSI
                     DYCDPEIPPIAHVEGIDLVTEGVITISRVVEYAKAYLSSNDISAPWANRKDGAAQIAQ
                     MLFEKATDINFFVGKAINPAHQNPNLPINFGIKIRLIQELAECLEKMGKHIKVSYF"
     gene            complement(4106..>4568)
                     /locus_tag="ACZPFK_04940"
     CDS             complement(4106..>4568)
                     /locus_tag="ACZPFK_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1405688.1"
                     /translation="ISNTPNAIFVLDEDLNIQQINQAALSLFNIPAGADLKGTPLVRI
                     LNPTDYLNTMVTGKSVLDKKYYLAEYKKYVAETIIYDKEYHMIFSIMRDITDAETKAS
                     QEDELRRETAEITDQVIEKQMRVVQEIASLLGETTAETKIALTKLKSTIQK"
BASE COUNT          775 a         1315 c         1464 g         1014 t
ORIGIN      
        1 ctgcggctgg tggggctggt gaacaaagcc gacagctatc ccgccatgct ctccggcggg
       61 cagcagcagc gggtcgccat tgcccgggcg ctggccatcg atccgcagat catgctcttc
      121 gacgagccta ccagcgctct ggacccggag ctgaccgcgg aggtgacggc ggtcatcgcc
      181 aagctggcgc agcaggatat cacgatgctc attgtcaccc atgagatgga gtttgcgcgc
      241 accgtggcgg accgcatcct cttttttgag gatggggaga tcatcgccgg gggcacggca
      301 aaagagtttt ttgagagcca gcagaacgac cgcatcaaac ggttcctcaa cggctttcaa
      361 aaatgaggcg gcggacctaa aataccgccg cgcaggggaa aagggcatcc cggcggggcg
      421 cggggtttat ggcttctccc gctgcggagg ggcagagggg ctttacagaa cgacagagag
      481 ggcgggcgct ttatagcgcc cgccctctct gtcgttccgg cacaaagcgg catgtcactt
      541 ttttcagggt cagacatgac ggaacacgat gccctccggg ctcatctcgt cgatgatggc
      601 caggctctgc aggttataac ccgcctcccg cagcttgtcg ccgccgccct gaaagccctt
      661 ttcaacggcg atgccgatgc cggccaccgt cgccccggcc tggcgggcga tgtccagcag
      721 gcccagctgc gccttgccgg tggcgagaaa atcgtccagg atcaggatgc ggtcctgcgg
      781 cgtcaaaaat ttcttttcca gcatggcggt gtagtcgttg ccgtgggtgt agctgcgtat
      841 gaccgaggtg taaacgtccc cgtcgatatt tttgctcttg gcctttttgg caaagacgat
      901 aggcacgtga aagtactggg cggcaatggc ggcaatggcg atgccgctgg cctcgatggt
      961 gacgatcttg gtgatcgtct cacctcggaa caggcggaaa aattcccgtc ccatctcgtt
     1021 catcagggcg atgtcgatct gatggttgag aaagctgtcc accttgagga tattgccctc
     1081 cttgaccttg ccgtccttta aaatgcgctg ctccaacaat tccataaaag cactcctgta
     1141 gtaaaagtgc gccccaccgg gcggcggacg ccgccggggg gcgcacagtt ctttcgtgcg
     1201 gttttgatag ggaatggccg ccgggttcca gcggaaaagc ccggccgccg cgccggcgca
     1261 gcccccatgt caaccgggcc cgcggcggtc cgcttgtcgg cagccggctc ttttctgcga
     1321 agaggcgatg cctcgaacgg ctgccctgtc gggcaagatc cgggcttttt tatcctcact
     1381 gcgcgttatg tagggctcat tccttttcgg ggctgacgcc gaacgcgatg cccgccgcgc
     1441 tctcaatggt tttttccagc gccaggcggc cgtattcgtc cacttcgttt ttatccttgg
     1501 ggccgtgggt caggcagccc gcgccgccga tgcagccgtt gatgcaggcc atgccctcaa
     1561 tgaagttgcc gggcagcagg ttgcggctgg ccttgagcag cgccagcttg cactcctcga
     1621 tgccgttgca gaccacgggc ttcgcctcaa aatccttgat gtcctgctcg cggaaagcct
     1681 ccgcgaccgc gtcggagagg ccgccgctgc gggcgaagat gcgcccgtaa taggaggcat
     1741 tgtccagtac gtcctcctcc agcgcggtca ggtcgatctc gcaggcctcg aacagggcgt
     1801 gcagctcttc aaaggtgagc acgctgtcca cataggggct cacctcgggc agctggaact
     1861 ccattttttt cgcggtgcag gggccgataa agaccacctt ggccgtggga tcggtggcct
     1921 tgacgaactt gcccaccgcg cccatggggg agaggtttga ggagattttg tccgccatgg
     1981 cggggaagtt ctttttgatg tagtccacaa aggccgggca gcaggagctg gtcaaaaaac
     2041 cacgctgtgc cagctcgcgg ctctccgcca gcgagaccat gtcggcgccg agggccgcct
     2101 cgatcacatt gtagaagccc agcaccttga ggccggagat gacctggccc agcttggcgt
     2161 agttgaactg gctggacacc gagggggcga ccacggcgta gaccttgtag ttctggttgt
     2221 ttttgctggc gcgcagcagc ttgacgacat ccacgataaa gctgcggtcc acaatggccc
     2281 caaacgggca catgtacacg caggagccgc aggagatgca cttgctgttg tcgatctcgg
     2341 cctttttctc ctcgttcatg tggatggcgt tcaccttgca ggcgttttcg caggggcggg
     2401 tgtgcttgct gatggcgttg taggggcaca ccgacgcgca cttgccgcat ccgacgcatt
     2461 tggagtggtc gatgatggcg tgctggtctt gaatggtgat cgcgccgcgc gggcagacct
     2521 cctcgcagcg gtgggcgatg cagccgcggc agtcggggcc caccgtgtaa ccgctcacgg
     2581 ggcactcgtc acaggcgata tccagcacct ggatcacgtt ggggttttcg gggtcgccgc
     2641 ccagggcgct gtggacgcgt tcgcccagca ccgcacgctc cttgtagatg cagcatcgca
     2701 tggtggcctt ggcgccgtcc gggatgatgg ccttggggac gtcccgatag gcgtcctgca
     2761 gggtatcggc ataggcgtgc ttggcgacct ccttgaggac cttgtacttc agccactgga
     2821 cgcgggtgtc aaaaattctt ccattcacgt tgcagattcc ttcctctcct gaacaccgcg
     2881 gcgcgccctg ctcggcaggg cgcacagtgc ggcgcctgtt atcagaaata gctgaccttg
     2941 atgtgcttgc ccatcttttc caggcattcg gccagctcct ggatcaggcg gatcttgatg
     3001 ccgaagttga tgggcaggtt ggggttctgg tgggcggggt tgatggcctt gccgacaaaa
     3061 aagttgatgt cggtggcttt ttcaaacagc atctgggcga tctgggcggc gccgtccttg
     3121 cggttggccc agggcgccga gatatcgttg ctggaaaggt aggcctttgc gtactccacc
     3181 acccggctga tggtgatgac gccctcggtc accaggtcaa tgccctccac atgggcgatg
     3241 ggggggatct ccgggtcgca atagtcaatg ctggtgttga tcggcttgcc caaaaactgg
     3301 gccgccaggg tactggtggt gccgccacag acgattttgg cgccctcttt ggagaagaag
     3361 aggttcatca tcatgcccac gtccttgggg tccgccggcg ggccgatcat caggttgacc
     3421 gggttgcgct ttttgatctt gacacaggcg atggtggtgt cgtcgcccgg ctcgccctcg
     3481 tacaggctgt tgcactcgtc cacgatcatg gtggagatgg tcttggcgga catcgaccaa
     3541 tcgaagatgg cctctgcaaa atcgacgatg ttctcgcgct gccagccgaa gttgagcgtt
     3601 ttgcccacgc ccgcgtagat ggcgccgtcg ctcatggcga taaaggtgtc gtccagctgc
     3661 agcgagatgg tgctctccag caccgttttg tcgccgatca cccggctgac gatggggtag
     3721 tccacgctct tgccgtcccg caggaaaatc acgttcgggt tgtcgaactg gatgatccgg
     3781 gccgtgtcgt tgtgctcgat gtggatgatg gtgaacgtgc tgtaggcgac cttgcgcacc
     3841 tggcagacgg gcagggtttt gacgatggtg tccacgcagt cctcgatgga catgcccgcg
     3901 tcgatcatcg tggagaggat cttggaggtg agtgtggaga ggatgttcgc cttgacgccg
     3961 ctgcccaggc cgtcggcgag aaccagggtc aggcagtcgt ccgccgtgcc gatgatctcc
     4021 accttgtcgc cgcacagctg ttcaccggtt ttgttgatgc tgacatagcc ggtgtcggtc
     4081 cacaggttgt tcatatgcag cctccctatt tctggatcgt cgatttgagc ttggtcagcg
     4141 cgatcttggt ctcggctgtc gtctcaccca gaagggatgc gatttcctgc acgacccgca
     4201 tctgcttttc gatgacctgg tcggtgatct cggccgtttc acggcgcagc tcgtcctctt
     4261 gcgaggcctt ggtctcggcg tcggtgatat cccgcatgat gctgaaaatc atgtggtatt
     4321 ccttgtcgta gatgatggtc tcggccacat attttttgta ctcggcgaga tagtattttt
     4381 tgtccagcac gctcttgccg gtgaccatgg tgttgaggta atcggtgggg ttgaggatgc
     4441 gcaccagcgg cgtgcccttg aggtctgcgc cggcggggat gttgaacagg ctcagcgccg
     4501 cctggttgat ctgctggatg ttcaggtcct cgtccagcac aaagatggcg ttgggggtgt
     4561 tgctgatg
//
LOCUS       DBMDYA010000150         4563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4569_length_4563_cov_1.528463, whole genome shotgun sequence.
ACCESSION   DBMDYA010000150 DBMDYA010000000
VERSION     DBMDYA010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_4563_cov_1.528463"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="ACZPFK_04945"
     CDS             <1..675
                     /locus_tag="ACZPFK_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025643.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR1405689.1"
                     /translation="SSIEEMGRDAKRKKRLIFWNRVAKRAAMILIVVVTALTAALSVK
                     GVRAQLYEYFVTHNDEYTQVQVAAADEGFRGRMVSEGDYHYEPSFVPARYILREKKEE
                     YDFFYHLEYVDKTAYAEFEQRQKALIEKWGSEDAIPEQERIYYDGPFLLFEEQRSIEG
                     SVIFDTEDAVYKELELDGSPAYLALKNGRATLIWDNGDRAFMLFGQLSEEEILRVARS
                     VARVEE"
     gene            1132..1482
                     /locus_tag="ACZPFK_04950"
     CDS             1132..1482
                     /locus_tag="ACZPFK_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIR1405690.1"
                     /translation="MLENLIELVLPPIIAATELIGIFVVTVAVLKAFFHYLVTLLGIR
                     GKRYAVKFELANGMATGLEFKMAAEILKTVQVRSFQELLVLGAIILLRALLSFMIHAE
                     MKNGPEETGTPPEA"
     gene            complement(1805..>2005)
                     /locus_tag="ACZPFK_04955"
     CDS             complement(1805..>2005)
                     /locus_tag="ACZPFK_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1405691.1"
                     /translation="VPGRPAKVQDTIGAGDSHIGAMIAGLAGGLSFQQAACNANVFAA
                     AVTSNPGAILPREVFESLSFDF"
     assembly_gap    2006..2015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2016..2718)
                     /locus_tag="ACZPFK_04960"
     CDS             complement(<2016..2718)
                     /locus_tag="ACZPFK_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1405692.1"
                     /translation="MKKTLVIGSTVADVIVRVDHLPRTGEDLDIDAQSLSLGGCAYNV
                     YHALELFGSPAMLFSPVGTGIYGDFVFHSLAAADIRSAAPRVAEENGCCYCFVEPDGE
                     RTFVCRYGAEYLFRREWFDALPQEEISGVYLCGLEVERSSGDLLIDYLEQHPEFTIYF
                     APGPRIGSIPAERLERLFRLRPLVHLNAGEALTVTGCNNLHRAVKSLCAATRGRVVVT
                     LGARGVLCAEGDALVE"
     gene            complement(2791..3174)
                     /locus_tag="ACZPFK_04965"
     CDS             complement(2791..3174)
                     /locus_tag="ACZPFK_04965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1405693.1"
                     /translation="MEYKVDDRQLDAALFLPFAGQVWPGEYDEGRTQAALSRTMNITA
                     YDGKALVGCLRILSDGYYFGTITELLVLPAYQGRGIGSALLRLARQHAPTLLYFGAQP
                     GVEAFYEKNGCRKGLQSYVIEKESQ"
     gene            3380..3721
                     /locus_tag="ACZPFK_04970"
     CDS             3380..3721
                     /locus_tag="ACZPFK_04970"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR1405694.1"
                     /translation="MASAFTQRIYEVVKQIPRGRVASYGQVAMLAGNPRGARGVGFAL
                     HRNPEHGVIPCHRVVFRDGSLCSGFAFGGEGEQRRLLEAEGVSFLPDGRVDLERCGWL
                     TGDPEKHLTKG"
     gene            complement(3708..>4563)
                     /locus_tag="ACZPFK_04975"
     CDS             complement(3708..>4563)
                     /locus_tag="ACZPFK_04975"
                     /inference="COORDINATES: protein motif:HMM:NF020476.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HIR1405695.1"
                     /translation="EGFVLVPSPYDPGRISRVSLSPAAVDCIVFWSKDPAPMLPRLPE
                     LDAKGYLYYFSFTITGAGAELEPGAPDTAAAVDTFRALSGLLGPARVDWRFDPILLGG
                     ERTPERVAEQFARLCAQLAPLTTRCILSFADPYAHLDKSLPPPPGDAQIEATAALLAP
                     IAERCGLPLFTCAERADLSRFGIKKGACIDPQKIAHLLGSPLKAVKDPGQRKACDCIQ
                     SVDVGVYDTCPSGCRYCYATAHPGAALRRYAAHDPAAPMLTGWPRGGEKIADRPMPPL
                     SSGQLTLW"
BASE COUNT          853 a         1340 c         1544 g          816 t
ORIGIN      
        1 tcctcgatcg aagagatggg gcgcgacgcc aagcgcaaaa aacggctcat cttctggaac
       61 cgggtggcca agcgggcggc catgatcctg atcgtggtcg tcaccgccct cacggcggcg
      121 ctctcggtaa aaggggtgcg ggcccagttg tacgagtatt ttgtcaccca caacgacgaa
      181 tacacgcagg tgcaggtggc ggcggcggac gagggcttcc gcgggcgcat ggtgtccgag
      241 ggggactatc actatgagcc ctcgtttgta ccggcacggt atatcctgcg ggagaaaaaa
      301 gaggagtacg acttctttta ccatttggaa tatgtggaca aaacggccta tgcggagttt
      361 gaacagcggc aaaaggccct gatcgaaaaa tggggttcag aggacgcgat ccccgagcag
      421 gagcgcatct attatgacgg cccgttcctg ctgtttgaag agcagcggtc catcgagggc
      481 tcggtgatat tcgataccga ggatgcggtc tataaagagt tggagctgga cggaagcccc
      541 gcctatctcg ccctcaaaaa cggtcgtgcg accctgatct gggataacgg cgaccgcgct
      601 ttcatgctgt tcgggcagct cagtgaggag gagatcctgc gggtggcgcg cagcgtggcg
      661 cgcgtagagg aataagaggc tgttccgccg gtccgggcaa aacggctttc aagacccctg
      721 cgggggagag tagccgcggg caggggcggg agtgcaggcc gtttcaaaat gggcggcaaa
      781 gaaaaagagg acgcctggcc gagctctttc tgctgcaatc catcccaaaa cagcgacagc
      841 cctgtgggaa ttatttcccg cagggctgtt tttgatgatt tgctgtgagc cgcgtccggt
      901 ttgccttgcc ggggaagagc gagggggagg gcggccagaa agcggccgcg ctcgaaaagc
      961 agcgcagctt ttttgaccgc gctctcagat cggacaagcg tcttcaaaag ccgaggctgc
     1021 cttttgccgc tttgagtcga aagagagggc ttttcaaaac gcaaaaaatg gggtaagata
     1081 gaacgacagt aaacccgtgc ccggcccagc cgggcggcaa aggagcgtcc tatgctggaa
     1141 aatttgatag aactggttct gccgcccatc atcgcggcca ctgagctgat cggtatcttc
     1201 gtcgtcaccg tcgcggtgct gaaagcgttt tttcattatc tggtcaccct gctgggcatc
     1261 cgcggcaagc ggtatgcggt caagttcgag ctggcgaacg gtatggccac ggggctggaa
     1321 ttcaaaatgg cggcggagat cctgaagacc gtccaggtgc gcagctttca ggagctgctg
     1381 gtgctggggg cgatcatcct gctgcgcgca ttgctcagct ttatgatcca cgcggagatg
     1441 aaaaacgggc cggaggagac ggggacgccg ccggaggcct gaaagggctc aaagccgtcc
     1501 ggaaaacgga gggagcgccg gggcaccgcg ctgccgaaaa cagatttttt gcgcgcctgt
     1561 gggagcaatc ctcagacgcc taaaattttt agggctgaga gcaactcctc tacgcccttg
     1621 ccgcgccggt cgcggttgcg gcggggaaag ggtggccgcg accccgctgg aacgccccgc
     1681 cgcttttcct gagcggggga ggcgcgcggt ttggacgatt tgagcgaggc ggcgattttg
     1741 tctcctttgc ccaaagcctt tttctaccag tcggcccggc gggaacggag cgacggccga
     1801 ccgcttaaaa gtcgaagctg aggctttcaa atacctcacg gggcaggatg gcgccgggat
     1861 ttgaggtgac ggcggccgca aacacattgg cgttgcacgc cgcctgctga aaagagagcc
     1921 cccccgcgag cccggcgatc atcgccccga tatgggagtc cccggcgccg atggtgtcct
     1981 gcaccttggc gggccgtcca ggtacnnnnn nnnnncctcg acgagcgcat cgccctcggc
     2041 gcagagcacc ccgcgcgcgc ccagggtgac caccacccgg ccgcgcgtgg cggcacaaag
     2101 gcttttcacg gcgcggtgca gattgttgca gccggtgacc gtcagcgcct caccggcgtt
     2161 cagatggacc agcggccgca ggcggaacag ccgctccagc cgctcggcgg ggatgctgcc
     2221 gatgcggggg cccggggcaa agtagatggt gaactcaggg tgctgttcca gatagtcgat
     2281 caacaggtcg ccgctggacc gctccacctc cagcccgcag aggtagacgc cgctgatctc
     2341 ctcctgcggc agggcgtcga accactcccg ccggaagaga tattccgccc cgtaacggca
     2401 gacaaaagtg cgctctccgt cgggctccac aaagcagtag cagcagccgt tttcctccgc
     2461 gacccggggc gccgcgctgc ggatatccgc ggcggcgagg ctgtgaaaga caaagtcccc
     2521 gtaaatgccg gtgcccaccg gcgaaaagag catggcgggc gagccaaaca gctccagcgc
     2581 gtgatacacg ttgtaggcgc agccgcccag ggacagcgac tgtgcgtcga tatccaggtc
     2641 ttcgccggtg cggggcaggt ggtccacccg cacgataacg tcggcgacgg tggagccgat
     2701 gacaagcgtc tttttcattc ctgatccatc caatctgttt cattttactg tcggtcgttc
     2761 cgtttgcccg cgggcggacc gggaccttga tcactgactt tctttttcta tgacatagga
     2821 ttgcaagccc tttcggcatc cgtttttttc gtaaaacgct tccacccccg gctgcgcgcc
     2881 aaaatacagc agggtggggg cgtgctgccg ggcaagccgg agaagcgcgc tgccgatccc
     2941 ccggccctga taggcgggga gcacgagcag ctcggtaatg gtgccaaaat aatagccgtc
     3001 cgagaggatg cgcagacagc ccacaagcgc tttgccgtcg taagcggtga tgttcatcgt
     3061 ccttgacagc gcggcctgtg ttctgccctc gtcatattcc ccgggccaga cctggcccgc
     3121 aaaggggagg aaaagggcgg cgtcgagctg ccggtcgtcc accttgtatt ccatcgtttt
     3181 ttgtcctccg tatctataaa tcgatcccgt taagtcgatc tcgctgtccg ctcctctccc
     3241 ccgggcgctg caaaggaaaa ggcggggcga gaaaggtgcg ccctgccgcc ccgcaagacc
     3301 ggtctttcgg cctttggggc gagaggaaag ggataagccg ttttccgtgg gcggcaatca
     3361 aaaaaacagg aggtttttta tggcgtcggc ctttacacag cgcatttatg aggtggtgaa
     3421 gcagattccg cgcggccgtg tggcctctta cggccaggtg gcgatgctgg cgggcaatcc
     3481 gcgcggcgcg cgcggggtgg gctttgcgct gcatcgcaac ccggagcacg gcgtcatccc
     3541 ctgccaccgc gtcgtctttc gcgacgggtc gctctgcagc ggcttcgcct ttggcggcga
     3601 gggggagcag cggcgtctgt tggaggcgga gggcgtctcc tttttgcccg acgggcgggt
     3661 ggacctggag cggtgcggct ggctgaccgg cgacccggaa aaacatctca ccaaagggtg
     3721 agctgcccgc tgctgagcgg cggcatgggc cggtcggcga tcttttcacc cccgcgtggc
     3781 cagccggtca acatgggggc ggctgggtcg tgggccgcat aacggcgcag cgccgcgccg
     3841 ggatgcgcgg tggcgtagca gtaccggcag ccgctggggc aggtgtcgta gacgcccacg
     3901 tccacgcttt gaatgcagtc gcacgctttg cgctgccccg ggtccttgac ggccttgagc
     3961 gggctcccca gcagatgcgc gatcttttgc gggtcgatgc aggcgccttt ttttataccg
     4021 aagcgggaca ggtcggcgcg ctcggcacag gtgaaaaggg gcaggccgca gcgctccgca
     4081 attggggcca gcagagcggc ggtggcctcg atctgcgcgt cgcccggcgg cggtggcagg
     4141 ctcttgtcca gatgagcgta agggtcggca aagctgagga tgcagcgggt ggtcagcggc
     4201 gccagctgcg cgcagaggcg ggcaaactgt tcagccaccc gctccggcgt gcgctccccg
     4261 cccagcagga tggggtcaaa gcgccaatcg acccgtgcgg gccccaacag gccggagagg
     4321 gcgcggaacg tgtcgacggc ggcggccgtg tcgggcgcgc cgggctccaa ttccgccccg
     4381 gcgcccgtga tggtgaagct gaagtaatag agatacccct ttgcatccaa ttcggggagg
     4441 cggggcagca tcggcgcggg gtccttgctc caaaagacga tgcagtccac agcggcgggg
     4501 gagagggata ccctgctgat gcgcccgggg tcgtaagggc ttggtaccag aacgaagccc
     4561 tcc
//
LOCUS       DBMDYA010000151         4522 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4609_length_4522_cov_1.467767, whole genome shotgun sequence.
ACCESSION   DBMDYA010000151 DBMDYA010000000
VERSION     DBMDYA010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4609_length_4522_cov_1.467767"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="ACZPFK_04980"
     CDS             <1..244
                     /locus_tag="ACZPFK_04980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1405696.1"
                     /translation="SAGYAGRFGHGLGHSLGIEIHEDPRCSPKDGTVLRAGMTMTIEP
                     GIYLPGQFGVRIEDAVVVTENGCEILGSSDKNLIVL"
     gene            442..999
                     /gene="efp"
                     /locus_tag="ACZPFK_04985"
     CDS             442..999
                     /gene="efp"
                     /locus_tag="ACZPFK_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1405697.1"
                     /translation="MISAGDFRNGVTFEEDGNVLQVVEFQHVKPGKGAAFVRTKTKNV
                     ITGAVLEKTYNPTAKFPTAYIERREMQYLYEDSGLYYFMDTETYEQTPLNESELGDAF
                     KFVKENEMVKVLSYKGKVFGVEAPNFVVLEVTKTEPGVKGNTATNTLKPAIVETGAEV
                     KVPLFINEGEMIRIDTRTGEYMERA"
     gene            1101..1541
                     /locus_tag="ACZPFK_04990"
     CDS             1101..1541
                     /locus_tag="ACZPFK_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HIR1405698.1"
                     /translation="MNLTEKSAYIKGLMEGLDLDSDKKEVKVIKALVDLVEDLALTVT
                     DLDEDVDQVYDELDAIDEDLTEIEDAVFEDDEDDEDLDDDEDMYEITCPSCGETVCVD
                     EDTLLSDDLSCPACGEKFEIDFDGEGDACEGCAGCGDKDDKEEK"
     gene            1908..2252
                     /locus_tag="ACZPFK_04995"
     CDS             1908..2252
                     /locus_tag="ACZPFK_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405699.1"
                     /translation="MAAFFLVLGMVFYFSRPPLPGNINRSCGEPTTSTADFSFQAEAG
                     ERIRFSLASDLRAEELEIVLYDPTGNAVKRLDEAKALETFAVLDTGGVCLLRAELRDL
                     VGRFKAAVYRAP"
     gene            2334..3317
                     /locus_tag="ACZPFK_05000"
     CDS             2334..3317
                     /locus_tag="ACZPFK_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR1405700.1"
                     /translation="MKALIYGYRADEEAAFDEFTARLGIEPVYLRENLNRETAARAQG
                     IEAVAVNAMSLLDRENLEQLARQGVRYIATRSIGFDNLDVEACRALGLKFANAPYSPH
                     SVADYTVMLMLMVTRKLKTIQRRALAQDYGFEGFQGRELHNLTVGVVGTGRIGRTVLG
                     DLAGFGPHCIAYDLYPSDALRGQVEYVGLDELFARADIITLHAPLQASSRHMVNAESI
                     AKMKDGVVLVNTARGELVDTAALIDALESGKVGGAALDVLEGEQAYTFADRRSGRIVS
                     RERAILEAMPNVLLTGHYAFYTDQAVSDEVRIALEALKQFTETGRADSQII"
     gene            3416..4294
                     /locus_tag="ACZPFK_05005"
     CDS             3416..4294
                     /locus_tag="ACZPFK_05005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1405701.1"
                     /translation="MYELHQVGENTFYIDCPAKMGVYRVAPEEAVLIDSGNDKEAGKK
                     VLKIFDAQGWRLKAVYNPHSNADHIGGNRLLADRTGCAVYGTEIENAFTRDPLLEPSF
                     LFGGYPPRELRGKFLMAQPSSPRSLTPEALPAGMTVFPLPGHFFGMVGFATDDGVCFL
                     ADCLSGENILEKYHVSFIYDVAAYLETLDRVEQMQARLFVPAHAAATADIRPTVHANR
                     AKVQEVAARITELCSAPQTFEQILQQLFKSYGLAMDFAQYVLVGSTVKSYLAWLHDTG
                     RLEARFEDSRLLWQAV"
BASE COUNT          918 a         1289 c         1507 g          808 t
ORIGIN      
        1 cagcgccggc tatgcgggcc ggttcggcca cggcctcggc cactcgctgg gcatcgagat
       61 ccacgaggac ccgcgctgtt cgcccaagga cggcacggtg ctgcgggcgg gcatgaccat
      121 gaccatcgag cccggcattt acctgcccgg gcaattcggg gtgcgcatcg aggatgccgt
      181 cgttgtgacg gaaaacggct gtgaaatatt gggcagttcc gacaaaaatc tgatcgtgtt
      241 gtaaggccag gggaagccgg tgggcggaca tccctgccga cggcccgcaa accgggggga
      301 aatgacggaa aaatggccct tttcagctct gcgccccttg cattgcaggg cgttttagga
      361 taaaattgat aaggtaaatg tgtggccctg cgcaagggtt cgggccgcgc gggcagtctg
      421 aatattttgg aggatgtttt tatgatttct gctggcgatt ttcgcaatgg cgtgactttt
      481 gaagaggacg gcaacgtgct gcaggtggtc gagttccagc acgtaaaacc gggcaagggc
      541 gcagctttcg tgcgcaccaa gaccaaaaat gtcatcaccg gcgctgtgct ggaaaagacc
      601 tataacccca ccgccaagtt ccccaccgct tacatcgagc ggcgtgagat gcagtacctg
      661 tatgaggaca gcggcctgta ctattttatg gacaccgaga cctatgagca gaccccgctc
      721 aacgagagcg agctgggcga cgcgttcaag ttcgtcaaag agaacgagat ggtcaaggtg
      781 ctgtcctaca agggcaaggt ttttggcgtc gaggccccca acttcgttgt gctcgaggtt
      841 accaagaccg agccgggcgt caagggcaat acggccacca acaccctgaa gcccgccatt
      901 gtggagaccg gcgccgaggt caaagtgccc ctgttcatca acgagggcga gatgatccgc
      961 atcgacaccc gcaccggcga gtatatggag cgcgcgtaaa agcggcttca caatcacggc
     1021 ttgatcgccc gccagggcgt gagcataaaa acgacagggg cgcgcagcgc ccctgtctta
     1081 gccaaaagga gggctacccc atgaacctga ctgaaaaatc cgcttatatc aagggcctga
     1141 tggaagggct cgacctggac tccgacaaaa aggaggtcaa ggtcatcaag gcgctggtcg
     1201 atctggtgga ggatctggcc ctcaccgtga ccgatctgga tgaggacgtg gaccaggtct
     1261 acgatgagct ggacgccatc gacgaggacc tgaccgagat cgaggacgcg gtctttgagg
     1321 acgatgagga cgacgaggac ctggacgacg acgaggacat gtatgagatc acctgcccgt
     1381 cctgcggcga gaccgtctgc gtggatgagg atacgctgct ctcggacgat ctgtcctgcc
     1441 cggcgtgcgg cgaaaaattt gagatcgact ttgacggcga gggcgacgcc tgcgagggct
     1501 gtgccggctg cggcgacaag gacgacaagg aagaaaaata aacagggcct gccggcaggg
     1561 cggccaaagg gggcgcgggg gttccgcgcc ctttctcttt gctttgggcg gcggggcggg
     1621 ggacgcgcgc gctgaaaacc gcgcgaaaga aattgaaatc ccgctgcggc tgcgctataa
     1681 tggaaacaac cacagagagg tggcgggccc cggccgcgcc gcctctttat cgtttcaggg
     1741 ccccctgacc tgcggcaggg gggcacatcg ccgccggcct cggcccgttt tcctgcgtcg
     1801 gacacgggcg tcccccgtgg cgccccgctg ccacggcagc cgccgggctc tggaaagcaa
     1861 gggggaggga aaggagaaaa cacaaaaaga aaaaaggcgt cctcgtattg gccgcgtttt
     1921 tcctggtcct cggaatggtc ttttatttct cccgcccgcc tttgccgggc aatatcaacc
     1981 gcagctgtgg ggagcccacg accagcaccg ccgacttttc ttttcaggcc gaggcgggcg
     2041 agcggatcag gttttccctt gcctccgacc tccgagcaga ggagctggag atcgttttgt
     2101 acgatccgac cggaaacgcc gtaaagcgct tggatgaggc caaagcgctg gagacctttg
     2161 cggtgctgga taccgggggc gtctgtctgc tgcgcgccga actccgtgat ctggtgggga
     2221 ggttcaaggc ggcggtttac cgggcaccct gaaagaggcg cggtctgccg acgcaggagt
     2281 ttccccgggc ggcgcgcaag aaaaaagaac tgcacaaaag gagttgggat gagatgaaag
     2341 cattgatcta cggctatcgg gccgacgaag aggcggcctt tgacgagttt accgcccggc
     2401 tgggcattga gccggtctac ctgcgggaaa atctcaaccg ggagaccgcc gccagggcgc
     2461 aggggatcga ggccgtcgca gtgaatgcga tgagcctgct ggaccgcgag aacctggaac
     2521 agctggcccg gcagggggtg cgctacatcg ccacccgcag catcggcttt gacaacctgg
     2581 atgtggaggc ctgccgggcg ctcggcctca aatttgccaa cgccccctac agcccgcaca
     2641 gcgtggcgga ttataccgtg atgctcatgc tgatggtgac ccgcaagctc aagaccatcc
     2701 agcgccgcgc gctggcgcag gattacggct ttgagggctt tcagggcagg gagctgcaca
     2761 acctgacggt gggggtggtg ggcaccggcc gcatcggccg caccgtcctc ggcgacctgg
     2821 ccgggttcgg cccccattgc atcgcctatg atctttatcc cagcgacgcc ctgcgggggc
     2881 aggtggaata tgttggcctg gacgagctgt tcgcccgcgc ggatatcatc acgctgcacg
     2941 cgccgctgca ggcgtccagc cgccatatgg tgaacgcaga gagcatcgca aagatgaaag
     3001 acggcgtggt gctggtgaac acggcgcggg gcgagctggt ggacacggcg gccctcatcg
     3061 acgcgctgga gagcggcaag gtgggcggcg ccgccctgga cgtgttggag ggggaacagg
     3121 cctatacctt tgccgaccgc cgcagcgggc gcatcgtcag ccgtgaacgg gcgattttgg
     3181 aggccatgcc caacgtgctg ctcaccgggc actatgcgtt ttacaccgat caggcggtca
     3241 gcgacgaggt gcgcatcgcc ctggaggcgc tcaagcagtt caccgagacc ggccgagcgg
     3301 attcgcagat catctgagca gccggccccg tgaaagcgag gaggaaaggc ggccgcccca
     3361 agatcgcgcg gcgggatttt tggggcgagc ggcagaaaaa agggaggcaa agcggatgta
     3421 cgaactgcac caggtgggag aaaacacctt ttatatcgac tgtcccgcaa aaatgggggt
     3481 ctaccgcgtc gcgccggagg aagcggtgct gatcgacagc ggaaacgaca aagaggcggg
     3541 caaaaaggtg ctcaagatct ttgacgccca gggctggcgg ctgaaagccg tctacaaccc
     3601 ccattccaac gccgaccata tcgggggcaa ccgcctgctg gccgaccgca ccggctgcgc
     3661 cgtttacggc accgagatcg aaaacgcttt cacccgcgac cccctgctgg agccctcttt
     3721 cctgttcggg ggttatcccc ccagggaatt gcggggcaag ttcctgatgg cgcagcccag
     3781 cagcccacgg tccctcacgc ccgaggcgct gcccgcgggg atgaccgtgt tcccgctgcc
     3841 tggacatttt ttcgggatgg tgggctttgc caccgacgac ggggtctgtt ttctggcgga
     3901 ctgcctctcg ggcgaaaata tcctggaaaa ataccatgtc tctttcatct atgacgtggc
     3961 ggcctatctg gaaacgctgg accgggtgga gcagatgcag gcgcggctgt tcgtgcccgc
     4021 ccatgcggcc gcgacggcgg atatccgccc cacggtgcac gccaaccgcg caaaggtgca
     4081 ggaggtggcc gcccgcatca cggagctttg cagcgcgccg cagacctttg agcagatttt
     4141 gcagcagctg ttcaaaagct atgggctggc gatggatttt gcccaatatg tgctggtggg
     4201 cagcacggta aaaagctatc tggcctggct gcacgataca gggaggctgg aggcccgctt
     4261 tgaggacagc cgcctgttgt ggcaggcagt ctgaacagag gacaaaagag ggaggcgctg
     4321 atgcgcctcc ctcttttgtc gtgaaaggat cattttttcg ccgcgtcctt tttgggcgcg
     4381 ttcttttttg cgtccgccaa ctgccgctcc agcagaatgg tggcaaagtc cagggcgttg
     4441 tagagcccgt ttttgtcggt ctgcttgatg atgcggtttt tgggggagag ggtggtgtcg
     4501 gtggagagca gggtcaggtg aa
//
LOCUS       DBMDYA010000152         4519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4613_length_4519_cov_1.728837, whole genome shotgun sequence.
ACCESSION   DBMDYA010000152 DBMDYA010000000
VERSION     DBMDYA010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_4519_cov_1.728837"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="ACZPFK_05010"
     CDS             complement(<1..707)
                     /locus_tag="ACZPFK_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1405702.1"
                     /translation="MEYDYDVGIVGAGPAGLAAAVYAARGGLSAICFEKEVHGGQIIN
                     SPEVENYPALGKVSGVEFATALYEQATGFGATVEYEEIVSADLEGEIKVLHGASRDWR
                     VRAVILAGGVQRRKLGCPGEDRLLGRGVSYCATCDGAFFAGKTVAVVGGGNTAVEDAL
                     YLATLCEKVVLIHRRQGFRATEAELAKLRANPKVEFVLDSAVAEVRGAERVESILVKS
                     TAGAPDREIAVAAVFVAV"
     gene            1066..2424
                     /locus_tag="ACZPFK_05015"
     CDS             1066..2424
                     /locus_tag="ACZPFK_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405703.1"
                     /translation="MAQSQSELGTKPIGRLLFKLAVPTVIAQLINVLYNIVDRIYIGN
                     IPGTGALALTGLGVTFPILMIISAFAAFVGMGAAPLASMQMGAGKNDKAEKILGTSTL
                     LLTILAAVLTTVFMIFKRPLLYLFGASDNIIGYAEDYITIYLLGTVFVMFSLGLNAFI
                     SAQGFATTAMLSVLIGAVSNIVLDPIFIFVLNMGVRGAALATIISQAVSAVWIVWFLL
                     GKKTHLRIRKENLRLTPALVGSIAALGVSPFIMQSTESLVNIVLNRGLQSYGGDLYVG
                     TLTIMTSLLQFIMLPTQGIAQGAQPIISYNFGAGNAERAKETFRKLLTVTLSFTVVCC
                     LAVVLLPSVFARIFTPDAQLLGMTAKVMPIYFAGIWAFGIQIACQITFLSLGQAKISL
                     FLALLRKIILLIPLALLLPEFFGVMGIYWAEPIADIAASCTSLVIFLAKRKQIFTRAE
                     TA"
     gene            2535..2897
                     /locus_tag="ACZPFK_05020"
     CDS             2535..2897
                     /locus_tag="ACZPFK_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1405704.1"
                     /translation="MPEATFYYKNPAAPRPNRPPILGACAIIQYRNTYLMESRADSDR
                     WAFIGGAMEPEESLEDCILREMREETGLVVAPGLLQFVGVYSDPSRIAAYPDGNITRI
                     ISAVYRVVLEDEPVLHCS"
     gene            complement(3094..3717)
                     /locus_tag="ACZPFK_05025"
     CDS             complement(3094..3717)
                     /locus_tag="ACZPFK_05025"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1405705.1"
                     /translation="MKKFVITIARENGSGGRAVGEKLARRLGVPFYNRDLLRLASDES
                     GISEQLFARADEEVKRTHLFRVAQDAYKGEVIPPDRDDFVSNENLFRYQARVILELAR
                     TENCVIIGRCADYILKDYENVVRVFVHAPLAARISTIMERHNLSEEAARREIARVDKR
                     RANYYRTFTGTDWRDASHYDLCLNSADLGMDKCVDLVAKFLDIRLGE"
BASE COUNT          792 a         1548 c         1314 g          865 t
ORIGIN      
        1 acggccacaa agaccgccgc cacggcgatc tcccggtcgg gcgcgccggc cgtgctcttc
       61 accaaaatgc tctcgaccct ctccgcgccg cgcacctccg cgacggcgga atccagcaca
      121 aattccactt tcgggttggc gcgcagcttg gccagctcgg cctcggtggc ccggaagccc
      181 tgcctgcggt ggatgagcac cactttttcg cagagggtgg cgaggtagag cgcatcctcc
      241 acggcggtgt tgccgccgcc caccaccgcc accgttttgc ccgcaaaaaa cgcgccgtcg
      301 caggtggcgc aatagctgac gccccggccc agcagccggt cctcacccgg gcagcccagc
      361 ttgcggcgct gcacgccccc ggccaggatc accgcccgca ccctccagtc gcgggaggcg
      421 ccgtgcagca ccttgatctc gccctccagg tcggctgaga cgatctcctc atactccact
      481 gtagcaccaa agccggtcgc ctgctcatag agggcggtgg cgaactcgac gccgctgacc
      541 ttgcccaggg cggggtagtt ttccacctcg ggcgaattga tgatctggcc cccatggacc
      601 tccttttcaa agcagatcgc cgagaggccg cccctcgccg catagacggc cgccgccagg
      661 cccgccgggc ccgcgccgac gatgcccacg tcgtaatcgt attccatttc gcactctccc
      721 gtccgttttc cgtcccgctt cccctcgcgg cggctccggc cccgcgcggg aaaaacgctt
      781 ttgcgtctat tataggcgaa tccccctgcc ccgtcaaaca ccgccgcggg ggccgcgcac
      841 ctgtccgacc tttggcctcc cgcaaaaatt ttttcaattt cagatagttc acaaaagaac
      901 tatcttgtat tttttgtccg cccgtcctat aataggtatc tggcggcccc tgcggctgcc
      961 ccccgcgccg tgcgcgcgtc gggcgagggc gggcgggttt gtcccctttt gcatttctat
     1021 gagcccgcgc cgcggattat gttgtctttc aaggaggtac ccccattggc tcaatctcaa
     1081 agcgagctcg gcaccaagcc catcggcagg ctgctcttca agctggccgt gcccaccgtg
     1141 atcgcccagc tcatcaacgt gctctacaac atcgtcgacc gcatctatat cggcaacatc
     1201 cccggcaccg gcgcgctggc gctcaccggc ctcggggtca cgttcccgat cctgatgatc
     1261 atctccgcct ttgccgcctt tgtgggcatg ggcgccgcgc cgctggccag catgcagatg
     1321 ggcgcgggca aaaacgacaa ggcggaaaag atcctcggca ccagcaccct gctgctgacc
     1381 atcctggccg cggtgctgac caccgtcttc atgatcttca agcggccgct gctctacctg
     1441 ttcggcgcgt cggacaacat catcggctac gccgaggatt acatcaccat ctatctgctg
     1501 ggcaccgttt ttgtgatgtt ctcgctggga ctcaacgctt tcatcagcgc ccagggcttt
     1561 gccaccaccg ccatgctcag cgtgctgatc ggcgcggtga gcaatatcgt gctggacccc
     1621 atcttcatct ttgtgctgaa catgggcgtg cgcggcgcgg cgctggccac catcatctcg
     1681 caggccgtca gcgcggtgtg gatcgtctgg tttctgctgg gcaaaaagac ccatctgcgc
     1741 atccgcaaag agaacctgcg cctgaccccg gcgctggtgg gctccatcgc ggcgctgggc
     1801 gtttccccct ttatcatgca gtccaccgag agtttggtga acatcgtgct caaccgcggg
     1861 ctgcaaagct acggcggcga cctgtacgtg ggcaccctca ccatcatgac cagcctgctg
     1921 cagttcatca tgctgcccac ccagggcatc gcccagggcg cacagcccat catcagctac
     1981 aatttcggcg ccggcaacgc cgagcgcgcc aaggagacct ttcgcaagct gctcacggtc
     2041 accctctctt ttaccgtggt gtgctgcctc gcggtggtgc tgctcccctc ggtctttgcc
     2101 cgcatcttca cccccgacgc ccaactgctg ggcatgacgg ccaaggtgat gcccatctac
     2161 tttgccggca tctgggcttt cggcatccag atcgcctgcc agatcacgtt cctctcgctg
     2221 ggacaggcca agatcagcct cttcctggcg ctgctgcgca agatcatcct gctgatcccg
     2281 ctggcgctgc tgctgcccga atttttcggg gtcatgggca tctactgggc cgagcccatt
     2341 gcggacatcg cggcctcctg cacctcgctc gtcatcttcc tggccaagcg caaacagatc
     2401 ttcacgcgcg ccgagacggc gtaaaccact cctgggcccg gcggcgcttc cccagttatt
     2461 tccccgtctt ttggctttgc aacgcccgcg gcttgtgtta aaataataac aggagccgaa
     2521 aggaggcgtc ccagatgccg gaagcgacct tttactataa aaatcccgcc gccccccggc
     2581 ccaacaggcc gccgatcctg ggcgcctgcg ccatcatcca ataccgcaat acctatctga
     2641 tggagagccg ggccgacagc gaccgctggg cctttatcgg cggcgccatg gagccggagg
     2701 aaagcctgga ggactgcatc ctgcgcgaga tgcgcgagga gaccggcctg gtggtggccc
     2761 ccgggctgtt gcagttcgtc ggggtctact ccgaccccag ccgcatcgcc gcttatcccg
     2821 acggcaacat cacccgcatc atcagcgcgg tgtacagggt cgtgctggag gacgagccgg
     2881 tgctgcactg cagctaggaa tccaagcagc ttcaattttt caccatcgag cagctggcgc
     2941 cgctcacggt cgccgagacc caactggaca tcctcagcga ttttatcgag gcgctgctct
     3001 gagcgcgccg cccgccgaaa gcaaaaacag cgcccggccc cgggttttac ccaggggccg
     3061 ggcgctctct tgtctcgcgc gccgtggcgg agttcactcg ccgaggcgga tatccaaaaa
     3121 ttttgccacc aggtcgacac atttgtccat gcccaggtcg gcggagttga ggcagaggtc
     3181 gtaatggctg gcatcccgcc agtcggtgcc ggtgaacgtg cggtagtagt tggcgcggcg
     3241 cttgtcgacg cgggcgatct cgcggcgggc ggcctcctcg ctcaggttat ggcgctccat
     3301 aatggtggag atgcgggcgg ccagcggggc gtggacaaat acccgcacca cgttttcata
     3361 atccttgagg atatagtccg cgcagcggcc gatgatgacg cagttttcgg tgcgggccag
     3421 ctccaaaatg acccgggcct gatagcggaa caggttctcg ttggagacga agtcgtcccg
     3481 atcgggcggg atcacctcgc ccttgtaggc gtcctgggcc acccgaaaca ggtgggtgcg
     3541 cttgacctcc tcgtcggccc gggcaaaaag ctgctcgctg atgccgctct cgtccgaggc
     3601 cagccgcagc aggtcgcggt tgtaaaaggg cacgcccagc cgccgggcca gcttttcgcc
     3661 gaccgcgcgg ccgccgctgc cgttttcacg ggcaatggtg atgacgaact ttttcatgat
     3721 gatccctcct ctgggtcagg gctgcggggc gggacgccgc gcccggcgga caggcccgcc
     3781 ccttgagagc gcggcccgcc tttttgacga cacggccttt tccaatttca gtatagagaa
     3841 ttcggagaac aaaatcaatg cgccgggcaa gtttttattg cgcggccctc aaaaacagcc
     3901 tgaggccgtt gtgcggcccg ccggccggcc cctctgcctg aggccatccg gccaaaaaac
     3961 cggcacggca gagcaaaggc ggtcggcgtc gccgcaccag tctttatttc cctttcgatg
     4021 caagcgggtg caagtgaaca aataggcccc tcccactcct gcccgacagc tcccctttca
     4081 ggggagccgg atggcggctg tttttcggct gagtgcagaa agggctcaat ataagaaagg
     4141 ggaaatctcc tatcctgtaa aaattttgga gagcgctctt ccctgctttc cccggctagg
     4201 cgcaacggcg cggcaaaagg atcgggccgc tgcgccggag gaaaagagaa atgcgcgcta
     4261 aaaagcagct gcaggccgct tttgttttct cctttcggtt accgtgccga gggcggaaaa
     4321 tgaggttctc ccgataagtc gttttttatg gtgaaacgat aaaaacagcc gcagcaggac
     4381 acaggctgtc ttttccgccc ataccgcccg ccgcgccggc gcggggcggc agacccgccc
     4441 cctctcccgg cgcggctgcg accggcgccc ccgcggcaaa catgaccgcc gcgccccgcg
     4501 cataaactaa aacaaaggg
//
LOCUS       DBMDYA010000153         4519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4614_length_4519_cov_1.430285, whole genome shotgun sequence.
ACCESSION   DBMDYA010000153 DBMDYA010000000
VERSION     DBMDYA010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_4519_cov_1.430285"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="ACZPFK_05030"
     CDS             <1..224
                     /locus_tag="ACZPFK_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405706.1"
                     /translation="YFNGPVSRRCAVIETDGRKNSLRFAAAIFYCSAPLCNKKLITKR
                     PCLAPAGQPARMREKIACGYFLLLHNKND"
     gene            complement(421..1173)
                     /locus_tag="ACZPFK_05035"
     CDS             complement(421..1173)
                     /locus_tag="ACZPFK_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HIR1405707.1"
                     /translation="MSDHKKVLLVGESWHVHTTESKGFDVFSYDYYEEATGYIKAALE
                     SVGIEFVHIPSHMVEYSFPKTAAELGQYDVVMFSDVGANTLNLPMDVFMRLHPTTNKL
                     EILREYVEQGGAFVMIGGYLSFQGIQARGAYKNTPVEELLPVELLPGDDRVEKSQGVR
                     PQVCQPQHPAVQGLPAEWPLLLGYNKLIPKEGAEVPVTVNGDPLVVLGEYGKGRACAF
                     ATDCAPHWAPVEFCTWQGYAPLWKNLVDWLTR"
     gene            complement(1231..1422)
                     /locus_tag="ACZPFK_05040"
     CDS             complement(1231..1422)
                     /locus_tag="ACZPFK_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405708.1"
                     /translation="MGYKASERHEKSIKNTKYAKGFTGQKESAAEVFSPESGAPHPLW
                     ALGEKAERFFLAGEKAPML"
     gene            1524..1736
                     /locus_tag="ACZPFK_05045"
     CDS             1524..1736
                     /locus_tag="ACZPFK_05045"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1405709.1"
                     /translation="MKYQVITEECFQDGAAIRCYGIACEGCCVHAVSRDRGFVESLAG
                     RLQQSDLSPLHLKDYVEDALLRRCLP"
     gene            1809..1973
                     /locus_tag="ACZPFK_05050"
     CDS             1809..1973
                     /locus_tag="ACZPFK_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405710.1"
                     /translation="MIGMEKSTSHTALMAALFFFIGLTVGFLFAPVRQGIQVKVCNNG
                     NQAPREGKKA"
     gene            complement(2284..3906)
                     /locus_tag="ACZPFK_05055"
     CDS             complement(2284..3906)
                     /locus_tag="ACZPFK_05055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1405711.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLRIAAQKLDPYI
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDENLNQYSNLTTGKVYWNVLNKE
                     RSGEYLGETVQVIPHITGEIKSFIYSVGQKTAADVVITEIGGTTGDIESQPFLEAIRQ
                     VAHEVGRQNCLFIHVTLVPYLKGSNEHKSKPTQHSVKELQSFGIMPDIIVTRSDEPIE
                     ESIRRKIALFCNVKPDCVIENLTLPMLYEAPIMLEKNGLARVVCRELGIECSPCDLAD
                     WNAMLQRAAAAARPVRIALVGKYTRLHDAYLSVNEALAHAGYENGAAVQIEWVDSEQL
                     TPETTGARLAGCDGVLVPGGFGDRGIEGMIQAARYARVNDVPYLGICLGMQIAVIEYA
                     RDVLGLADANSSEFAPDSAHKVIDLMPDQHGNLPKGGTMRLGAYPCNIAPGTLMERAY
                     RSAQIGERHRHRYEFNNEYRRQLAEAGLVISGASPDGRLVEAVEEPANRFFVGVQYHP
                     EFKSRPHRAHPLFREFIAASLEKREEMQAVKN"
     gene            complement(3998..>4519)
                     /locus_tag="ACZPFK_05060"
     CDS             complement(3998..>4519)
                     /locus_tag="ACZPFK_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1405712.1"
                     /translation="QLAGLFTQGPRPSVATAASGGDARRALLGAEYDLVLINTPLRDE
                     FGHELAAQAAQRGAGVLMLVRAGLEEEVATRVEDWGVLVLPRPLNRTAFYKSIKLLQA
                     ARRRVWHLLEENRRLREKMEELRVVARAKCVLIERLGLTEEQAHHCIEKQAMDRRATR
                     RSVAEEILETCET"
BASE COUNT          771 a         1456 c         1331 g          961 t
ORIGIN      
        1 cttattttaa tggccctgtt tctcgccgct gcgcggtaat cgaaacagat ggccgaaaaa
       61 atagcctccg cttcgctgcg gctatttttt attgctccgc tccgctttgc aataaaaaac
      121 tgatcacgaa gcgcccgtgc ctcgcccctg cggggcaacc ggcccggatg cgcgaaaaaa
      181 tagcctgcgg ctatttttta ttgctgcaca ataaaaacga ctgatctcaa tggccgtggc
      241 ccgaggacgg gccacatggt cgaaaaaaac ggccgtaggg gccgttttaa tccacggtct
      301 taaactcttg agcggccgcc gagcgggcgg atgcgcaaaa aaagatgccg cgggcttccg
      361 cggcatcttt ttttatcggt ttctgcgtgt ccctatcacg cgggcgcgcc ctctcccggc
      421 tcagcgcgtc agccagtcca ccaggttttt ccacagcggg gcataaccct gccaggtgca
      481 gaactccacc ggggcccagt ggggagcgca gtcggtggca aaggcgcagg ccctgccctt
      541 gccgtactcg cccagcacca ccagcgggtc gccgttcacc gtcaccggca cctcggcccc
      601 ctctttgggg atcagcttgt tatagcccag cagcagcggc cactcggcgg gcagcccctg
      661 taccgccggg tgttgcggct ggcagacctg ggggcgcacg ccctggctct tttccacccg
      721 gtcgtccccg ggcagcagct ccacgggcag cagctcttct accggcgtgt ttttgtaagc
      781 gccccgcgcc tggatgccct gaaaagagag atagccgccg atcatgacaa acgcgccgcc
      841 ctgctccacg tactcccgca aaatctccag cttgttggtg gtggggtgca gcctcataaa
      901 gacgtccatg ggcaggttca gggtgtttgc gcccacgtcg ctgaacatca ccacgtcgta
      961 ctgccccagc tccgccgccg ttttggggaa gctgtactcc accatatggg aggggatgtg
     1021 gacgaactct atgcccacgc tctccagcgc cgccttgata tagccggtgg cctcctcgta
     1081 atagtcgtag ctgaacacgt cgaacccctt ggattctgtg gtgtgtacat gccagctttc
     1141 ccccaccagc aagacctttt tgtgatcgct cataaagacc tccttgtcct gttctgcgcg
     1201 cttctgcgct ctgctgtcgt tctaacggga ttatagcatg ggggctttct ctcccgcaag
     1261 aaaaaagcgc tctgcctttt cgcccagcgc ccataaggga tgcggggcgc cggattccgg
     1321 cgaaaaaacc tctgctgcgc tttctttttg tcccgtgaag ccttttgcgt attttgtatt
     1381 ttttatgctt ttttcatgtc tttctgacgc tttatatccc atttattgac atctcagcga
     1441 tttcatgata ctaaattact gttccctccc cgctcttcct gttccgagag ggggagggag
     1501 gaatctgggt ctggagggtc aggatgaaat accaagtcat aacggaagag tgttttcagg
     1561 acggcgccgc gatccgctgc tatgggatcg cctgtgaggg ctgctgcgtc cacgcggtct
     1621 cgcgggatcg gggatttgtg gagagcctgg cgggcaggct gcagcagagt gacctctccc
     1681 cgcttcacct caaagactat gtggaggacg ccctgctgcg gcgctgcctg ccctgacccg
     1741 cgggagcgcg cggccttttt taaaaagcgc tttctttccg tccgccggga tgctatactg
     1801 ggtgcgaggt gatcgggatg gaaaaaagta caagccacac cgccttgatg gcggcgctct
     1861 tcttttttat cggcctgacg gtgggttttc tgttcgcccc cgtgcggcag ggcatccaag
     1921 tcaaagtgtg caacaatggc aatcaagcgc cgcgcgaggg caaaaaagcc tagctttttt
     1981 ccggcaaagg cgccgctgca tactgcaaca aataaaaaag ctcccctgcg ggggagcttt
     2041 tttatttgtt gcacaaggca atatgcgcgc gggggacggg cttttcacgg cgcgtcgggc
     2101 cgcccatgaa aggcggcttc ctctcgccgc tctgcctcgc cgcatgcctc ctctggccga
     2161 gtaagcggtc gcggagcagt tggggtgagc cgtcctctgc gcaaaaaaat cttttgcggc
     2221 agcgctgttt ccgctgtatg gcctcgggca aaacagaaga tatccccggt aaaacagcct
     2281 atctcaattt tttaccgcct gcatctcttc tcttttctcc aggctggctg cgatgaactc
     2341 gcggaacagc ggatgcgccc ggtggggtcg gcttttgaac tccgggtgat attgcacgcc
     2401 gacaaaaaag cggttggcgg gctcttccac cgcctccacc agtctgccgt ccggcgaggc
     2461 gcccgagatc accagccccg cctccgcaag ctggcggcgg tactcgttgt tgaactcgta
     2521 gcggtggcga tggcgctcgc cgatctgggc ggagcggtag gcacgctcca tcagggtgcc
     2581 gggcgcgata ttgcagggat aagcccccag acgcatcgtg ccccccttgg gcaggttgcc
     2641 gtgttggtcc ggcatcaggt cgatgacctt gtgggcggaa tcgggtgcga actcgctgga
     2701 attggcgtct gcaaggccca gcacgtcgcg ggcgtattcg atcaccgcga tctgcatgcc
     2761 caggcagatg ccaaggtagg ggacgtcgtt gacgcgggcg tatcgtgcgg cctggatcat
     2821 gccctcaatg ccccggtccc caaagccccc gggcaccagc acaccgtcgc agccggccag
     2881 ccgcgcgcct gtggtctccg gcgtcagctg ctcgctgtcc acccactcga tctgcaccgc
     2941 cgccccattt tcataccccg cgtgggccag cgcctcgttc accgagaggt aggcgtcgtg
     3001 cagcctggtg tacttgccca ccagcgcgat gcgcaccggc cgtgcggccg cggcggcgcg
     3061 ctgcagcatg gcgttccagt ccgcgaggtc gcaggggctg cactcgatgc ccaactcgcg
     3121 gcagaccacc cgggcgaggc cgttcttttc cagcatgatg ggggcctcgt acagcatcgg
     3181 cagggtcagg ttttcgatga cgcagtccgg cttgacgttg cagaacagcg cgatcttgcg
     3241 gcggatggac tcctctatcg gctcgtcgct gcgggtgacg atgatgtccg gcatgatgcc
     3301 gaaagattgc agctctttta ccgagtgctg ggtgggtttt gacttgtgct cgttgctgcc
     3361 cttgagatag ggcaccagcg tgacatggat aaacaggcag ttttggcgcc ccacctcgtg
     3421 ggccacctgc cggatggcct ctaggaacgg ctggctctcg atgtcgccgg tggtgccccc
     3481 gatctcggta atgaccacgt cggcggcggt cttctgcccc acagagtaga tgaagctttt
     3541 gatctcgccg gtgatatgcg ggatgacctg caccgtctcg cccagatatt cgccgctgcg
     3601 ctccttgttg agcacgttcc aatagacctt gccggtggtg aggttggaat actggttcag
     3661 gttttcgtca atgaagcgct cgtaatggcc caggtccagg tcggtctccg cgccgtcctc
     3721 ggtgacgaac acctccccgt gctgataggg gctcatggtg cccgggtcca cattgatata
     3781 cgggtccagc ttctgcgccg cgatgcgcag gccgcgcgct ttgagcagcc tgccaaggct
     3841 cgccgcggtg atccccttgc ccagaccgga caccacgccg ccggtcacaa aaatatattt
     3901 tgtcatgccc tctctcctcc ctttttgctc aaagctcccg ctgagcgctc actgtgggca
     3961 gcgcgcccat ccgccctgaa aacagatccg tcgcggctca cgtctcacag gtctccaaaa
     4021 tctcttccgc cacgctgcgg cgggtggcgc gccggtccat cgcctgcttt tcgatgcagt
     4081 gatgggcctg ctcctcggtg aggcccaggc gctcgatcag cacgcatttt gcccgcgcca
     4141 cgacgcgcag ctcctccatc ttctcgcgca ggcggcggtt ctcctccagc agatgccaga
     4201 ccctgcgccg ggccgcctgc agcagcttga tgctcttata aaaagcagtg cggttcagcg
     4261 ggcggggcaa caccaaaacg ccccagtcct ccacccgcgt ggccacctct tcctccaacc
     4321 cggcacgcac cagcatcagt acccccgcgc cgcgctgcgc cgcctgcgca gcgagctcat
     4381 gcccgaactc atcccgcagg ggggtgttga tcagcaccag atcgtactcg gcgcccagca
     4441 gcgcccgccg cgcatcgccg ccgctggcgg ccgttgccac cgacggccgg ggcccctgcg
     4501 tgaagagccc ggccagttg
//
LOCUS       DBMDYA010000154         4506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4636_length_4506_cov_1.725085, whole genome shotgun sequence.
ACCESSION   DBMDYA010000154 DBMDYA010000000
VERSION     DBMDYA010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4636_length_4506_cov_1.725085"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..84)
                     /locus_tag="ACZPFK_05065"
                     /pseudo
     CDS             complement(1..84)
                     /locus_tag="ACZPFK_05065"
                     /inference="COORDINATES: protein motif:HMM:NF027782.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5048 domain-containing protein"
     gene            complement(100..1305)
                     /locus_tag="ACZPFK_05070"
     CDS             complement(100..1305)
                     /locus_tag="ACZPFK_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405713.1"
                     /translation="MAYIPTSEDIALLSQHSREIYSRIDILNKDFKTIDSVQGVTLDG
                     TVSIDAESDIRRTYNSTIHLADNNYITAENALIWMDKYVQIYLGVKGPGSQKPSWYSL
                     GLFKFCTNGFNYSQTDKTLSVSCVDLTATLDGTLSGELTGLSTEISEGETIREVLVRL
                     VHQLGGYDKYLISYADKKVPYDLKYGAASSIYSIVKEVRDLFYLYETFFDGRTFVCQE
                     VPTCEDDPVFLPAEVLDPLVVSESTNSDFSVIRNCTEVWGKVLTATYSAGSSTLSEGV
                     YALSVEQYKQRQKSRVSFIPPESNPASALIQINEEEACPLVDEEGRPLAAGTMVGGKL
                     CVVCYEDGKFFYLGQSQAHAMVMLVGREPTPEEIAEAKRAEGCDNLKFIVLPESPFTI
                     EKIGHKNQV"
     gene            complement(1311..2210)
                     /locus_tag="ACZPFK_05075"
     CDS             complement(1311..2210)
                     /locus_tag="ACZPFK_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405714.1"
                     /translation="MSFLGADFFGDDASFDAAVAAPAPFTAVTVQNGVYDELFITKNP
                     DQPTEPTAEKEWDYDTILYAKFDGTTTAGNIQYTVDQVSAMRIKYRESGTYDWITCYE
                     LPVRTDADFSFSKELKFLRSGVTYDVAIVPVLNGTEGNLNIASVKSEFDGLYLMEKEQ
                     SFHAPLNVSIYPISKNQSSSVITTLDGAYPFVLYNGKAKHRSGSASGLFVEPGQQDYD
                     FDNAENYRDRLLEFMTNGKPKLLKTYHGKEYIVNIIDSPQEERDGHDSNISLSFTWVE
                     TGDCSSSADLYQSALIDVLVEGG"
     gene            complement(2212..3291)
                     /locus_tag="ACZPFK_05080"
     CDS             complement(2212..3291)
                     /locus_tag="ACZPFK_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405715.1"
                     /translation="MALTKPSITQIAAFDASDEHVFVFSYAGDQFEKSKAVIKDNNTD
                     EVVFEGVTTSWQARFVLPAGTLSNSASPYSIAVCVIDKAGAEGPYSDPLLFFCRSAPQ
                     FSFLNLSDGQTIGASSYVAGLNYAQNEGELLDSFQFVLYSANKARIKESGLLYDTASM
                     QYTLLNLQDRETYYVEAVGETVNHMQLSTGLVRIVVSYITPAAFSSVVLTNRKQNGDV
                     LVSSNVISIDGRPTPAPPVYLDNKRIDLSAPGAKVDFSAGFSINEDFLLSLIVAGPVP
                     YETLLSLSGENRVSVRYMENHFSGMPAPVAYMAVQISSQEGGVYLVNSNYIPLPDYTA
                     KQLHFCLKRVGAFYTAELSAIEKEG"
     gene            complement(3431..>4506)
                     /locus_tag="ACZPFK_05085"
     CDS             complement(3431..>4506)
                     /locus_tag="ACZPFK_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025253.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR1405716.1"
                     /translation="WPDGGPIDPVAWPELHAYAARLGWQTDAAGHFLLPDLRGKYLVG
                     ADSRDVDFAAGTVGGEKTVALTAENNGPHTHLLFSSSGGTVWNGFGSAAKGDVNSPVP
                     TSVSGQGAPHNNLPPYRAVYIQLRAKADPVVAQEVAADRLATARKIGSADFDGSADIT
                     LAQMGAATAAQGEKADSAMPKTGGIFSGPIIASGITSKAAMAVNEQLFLSALTQNYGI
                     KAEAASGYTMVWGMAGAKITDTNGTLKPVLASNISGSSSRRYKHKIRDMDEDTAKRLL
                     QLRPVGFEYKKELNDPGPKYGLVAEELTEIDPTCVYKGGNGQVEGINYTMLVPQLIKL
                     CQIQQDQIDALERRLSALETKEG"
BASE COUNT          875 a         1272 c         1313 g         1046 t
ORIGIN      
        1 ggagaccgaa tccgtcaggc gggtcgattt ccagttctca tattccgccc gctgcatcgc
       61 cagcgcatcc gaggtgatgt gatcgtattc accgccctgt cagacctgat ttttgtggcc
      121 gatcttttca atggtgaaag ggctctccgg gaggacgatg aatttcaggt tatcgcagcc
      181 ctcggcgcgc ttcgcctcgg caatttcctc gggggtgggc tccctgccca ccagcatgac
      241 catcgcatgc gcctgcgact ggccaaggta aaagaatttg ccgtcctcat agcatacgac
      301 gcagagcttg ccccccacca tcgttcctgc cgccaggggc ctaccctcct catccaccag
      361 cgggcaggcc tcttcttcgt taatctggat caaggcggac gccgggttgc tctcaggggg
      421 gataaagctg acgcgcgact tttgacgctg cttgtattgc tcgacgctca gggcataaac
      481 cccctcggac agggtggacg agccggcgga gtaggttgcg gtgaggacct ttccccacac
      541 ttcggtacag tttcggatga cggaaaagtc gctgtttgtg gattccgaga cgacgagcgg
      601 gtccagcact tccgctggga gaaagacggg gtcgtcctcg caggtgggca cctcctggca
      661 gacaaaggtc cgcccgtcaa aaaacgtttc atagaggtaa aacagatcgc gtacttcctt
      721 gacgatgctg taaatggaag aggccgcgcc gtattttaga tcatacggca cctttttgtc
      781 ggcataggag atcagatatt tgtcgtaacc tcccagctga tggaccagcc tgaccagcac
      841 ctcgcggata gtttccccct cgctgatctc ggtggaaagc ccggtgagct ctcccgacag
      901 ggtgccgtcc agcgtcgccg tcaggtccac acaggagacc gagagggtct tgtccgtctg
      961 gctgtagttg aacccgttgg tgcaaaattt gaacaggccc aaggagtacc agctcggctt
     1021 ttgcgagccc gggcccttga cgcccagata gatctgcaca tacttgtcca tccatatcag
     1081 cgcgttttcg gccgtgatgt agttgttgtc cgccagatgg atcgtcgagt tgtaggtccg
     1141 gcggatgtcc gactccgcgt cgatggagac ggtaccgtcc agcgtgaccc cttgcacgga
     1201 atcgatggtt ttgaaatcct tgttcaggat atcgatgcgg ctgtagatct cccggctgtg
     1261 ctgcgagagc agggcgatat cttcagaggt ggggatgtaa gccatattcc ttatcccccc
     1321 tccaccaaca catcgatcag cgcgctttgg tagaggtcgg ccgagctgct gcaatcaccg
     1381 gtctcgaccc aggtgaaaga gagactgatg ttggaatcgt gaccgtcccg ttcctcctgc
     1441 gggctgtcga tgatgttgac gatatattct ttgccgtgat aggtcttgag cagcttgggc
     1501 ttcccgttag tcatgaattc cagcaggcgg tcccgataat tttcggcgtt gtcgaaatcg
     1561 tagtcctgct gacccggttc tacgaaaagg ccggaggcgg acccgctgcg gtgcttcgcc
     1621 ttgccgttat agagaacaaa aggataggcc ccatccagcg tggtgatgac gctggagctc
     1681 tgatttttcg agatggggta gatcgagacg ttgagcggcg cgtggaagct ctgttctttt
     1741 tccatcagat acaagccgtc aaactcactt ttcaccgaag cgatgttcag gttgccctcc
     1801 gtgccgttga ggacgggcac gatcgccaca tcgtaggtga cgccgctgcg caggaatttc
     1861 agctcttttg aaaaagaaaa atccgcatcc gtgcgcaccg gcagctcgta acaggtgatc
     1921 cagtcataag tgccgctctc acggtacttg atccgcatcg cggagacctg atcgacggtg
     1981 tactggatgt ttcccgccgt ggtcgtgccg tcgaattttg catagaggat ggtgtcgtag
     2041 tcccattcct tttctgcggt cggctctgtg ggctggtccg gattttttgt gatgaacaat
     2101 tcgtcataga ctccgttctg cacggtgacc gcggtgaaag gcgcgggtgc ggcgacggcg
     2161 gcgtcgaaag aggcgtcgtc accgaaaaaa tctgcgccga gaaaagacat tttacccctc
     2221 cttttctatt gcggataact ctgcggtata aaaggcgccg acccttttca ggcagaaatg
     2281 cagctgtttt gcggtgtaat ctgggagcgg gatgtagttg gagttcacaa gatagacccc
     2341 tccctcctga gaggagatct gtaccgccat ataggctacg ggcgccggca tcccgctgaa
     2401 atggttttcc atgtagcgga cggaaaccct gttttccccc gagagggaaa ggagcgtctc
     2461 ataggggact gggcccgcaa cgatcaggga caacaaaaaa tcttcgttta tggaaaaacc
     2521 ggcggaaaaa tcaacttttg cccccggggc ggagagatcg atcctcttgt tgtcaagata
     2581 gacgggcggc gcgggcgtgg gcctgccgtc gatggagatg acgttgctgg acaccagcac
     2641 gtcgccgttc tgcttgcggt tggtcaggac gactgaactg aatgcggccg gcgtgatata
     2701 ggagacaacg atccggacga ggccggtgga cagctgcata tggttgaccg tctcgcccac
     2761 ggcctccacg taataggtct cccggtcctg caggttgagc agggtatact gcatagaagc
     2821 cgtgtcgtag agcagcccgc tctctttgat cctcgccttg tttgcggaat acaggacaaa
     2881 ctggaatgag tcgagcagct cgccctcgtt ttgtgcatag ttcagccccg ccacataaga
     2941 ggacgcgccg atggtttgac cgtccgacag gttcaaaaaa gaaaattgcg gggctgacct
     3001 gcaaaaaaac aacaggggat cgctgtaggg gccctctgca ccggccttgt cgatgacgca
     3061 gaccgcaatg ctgtaggggc tggcggaatt ggacagcgtc cccgccggca gaacaaagcg
     3121 tgcctgccag ctggtggtga ccccctcgaa cacgacctcg tcggtattgt tgtctttgat
     3181 gacggctttt gatttttcaa attgatctcc ggcataagag aagacaaaaa cgtgttcgtc
     3241 ggaagcgtcg aacgcagcta tttgagtgat gctgggtttt gttaaagcca aggcttcacc
     3301 tccttgtaat aaaacgattc aatttgtttt tctttgtact tcggcacagc cgggcgctgt
     3361 cccgggccgt gagccgcggt ctgcgcctgg gcggcgcacg gaactgtacc ggcagggatg
     3421 cttttacgcg ctaaccctcc ttggtttcaa gagctgacag acgccgctcc agggcgtcga
     3481 tttgatcctg ttgtatctgg cacagcttta tgagctgcgg caccagcatc gtgtagttga
     3541 tgccctccac ctgtccattg ccgcccttat agacgcaggt gggatcgatc tctgtcagct
     3601 cctccgcgac gagaccatat tttggccccg ggtcgttcag ctcttttttg tattcaaagc
     3661 ctactggccg cagttgcagc aatcgctttg ccgtgtcttc gtccatgtcc cgtatcttgt
     3721 gcttatacct gcggctggac gagccggaga tgttggaggc taacacaggc tttaacgtgc
     3781 cgttcgtatc ggttattttt gcgcccgcca tgccccaaac catagtgtag ccggatgctg
     3841 cttctgcttt tatcccatag ttttgcgtga gggcactcaa aaagagttgt tcgttgactg
     3901 ccatcgctgc tttggatgtg atgccggatg cgataatggg gccgctgaat atccctcccg
     3961 ttttgggcat cgcgctgtct gctttctcac cttgtgccgc cgtggccgcc cccatctgcg
     4021 ccagcgtgat atccgcgctg ccgtcaaaat cggcgctgcc gatcttgcgc gctgtggcca
     4081 gcctgtccgc cgccacctcc tgcgccacca ctgggtcggc cttggcgcga agctggatgt
     4141 acaccgcgcg gtagggcggc aggttattgt ggggggcgcc ttgaccggaa accgatgttg
     4201 ggacagggga attgacgtct cctttcgcgg cggagccaaa accattccac acagttcccc
     4261 cgctgctgct gaaaaggaga tgagtatgcg gcccgttgtt ttccgccgtg agcgccaccg
     4321 tcttttctcc gcccaccgtc cccgctgcaa agtctacgtc gcggctgtcg gcgcccacca
     4381 gatatttccc ccgcaggtcc ggcagcagaa aatgccccgc cgcatcggtc tgccagccca
     4441 gccgcgcggc gtaggcgtgc agctccggcc aggccaccgg gtcgatgggg ccgccgtccg
     4501 gccaga
//
LOCUS       DBMDYA010000155         4488 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4655_length_4488_cov_1.551630, whole genome shotgun sequence.
ACCESSION   DBMDYA010000155 DBMDYA010000000
VERSION     DBMDYA010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_4488_cov_1.551630"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="ACZPFK_05090"
     CDS             complement(<1..628)
                     /locus_tag="ACZPFK_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1405717.1"
                     /translation="MAEPEHWVYHRTGLSYGRPEGLAEAVEEIFRLCPAAGRLGPGSR
                     VLLKPNLLAKHPPEHAVTTHPAVVEAVVAALRRRGVEHIVLADSCGGVYSEGVMRGIY
                     RASGLAAACERLGVEVWSKTGSGARASGGRLVREFNLITPVLESDFIIDLPKFKTHVM
                     TGLTAAVKNLFGCIPGLQKAEMHMRFPDRERFGEMLCDLCGAVRPALVI"
     gene            complement(621..1796)
                     /locus_tag="ACZPFK_05095"
     CDS             complement(621..1796)
                     /locus_tag="ACZPFK_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1405718.1"
                     /translation="MEYSKLLSARAQALKPSGIRKFFDVAGTMEGCISLGVGEPDFKT
                     PWMIRDAGIKSLERGKTWYTSNAGLAEMRAEIAAYLERRFELHYDPQSEMLVTVGGSE
                     AIDLALRAVLEPGDEVIIPEPCYVSYAPLTSLVGGVPVPVPLKAENGFRLTAEELRAK
                     ITPRTKILVLPFPCNPTGAVLRERHLAALAEVLQDTDILVLTDEIYAELTYGARRHVS
                     IASLPGMKERTIYINGFSKAYAMTGWRLGYVCAPAPLMKAMTKIHQYAIMCAPTTSQY
                     AGIVALREGDDAIEAMRDEYDMRRKFLVSALNRMGMTCFEPEGAFYVFPSIAVSGLRS
                     EEFCEKLLFSKKVAVVPGTAFGDSGEGFVRISYSYSVAHLSEALGRIEEFLKECGHG"
     gene            complement(1796..2275)
                     /locus_tag="ACZPFK_05100"
     CDS             complement(1796..2275)
                     /locus_tag="ACZPFK_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1405719.1"
                     /translation="MDRILKILEDNARLPLEDIAAMVDKTPEEVAAAIDEYAKSGIIK
                     GYKTLVDWDKTDSVRVRALIELKVSPKKDHGFDEIASTIALFPEVDSVMLMSGGYDLG
                     LILTGKSFQEIALFVALRLSPLDDVLSTATHFVLRTYKRDGVMYTDENRDERGYNFL"
     gene            complement(2336..2518)
                     /locus_tag="ACZPFK_05105"
     CDS             complement(2336..2518)
                     /locus_tag="ACZPFK_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405720.1"
                     /translation="MAEKQLILMAPFLAAVAATETDGRKNSLRLFFIIPDNARALNGQ
                     APGQPLSKAGFASIMS"
     gene            2586..2855
                     /locus_tag="ACZPFK_05110"
     CDS             2586..2855
                     /locus_tag="ACZPFK_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1405721.1"
                     /translation="MKAVVTVVGRDTVGILAKIAGVCAETNVNVEEVTQSILSGTFAM
                     IMVVDVTACTIDFAELQRRFAAAGEEKGVDVKVTRQELYDAMHHI"
     gene            2882..4243
                     /locus_tag="ACZPFK_05115"
     CDS             2882..4243
                     /locus_tag="ACZPFK_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1405722.1"
                     /translation="MLDTKDILETIRMISDENLDVRTVTMGISLLDCADEDPHRACEK
                     IYRKITDRARDLVRTAEEVSAAYGVPIVNKRISVTPIAMLLASCKDPDPVEFAMTLDA
                     AAKKVGVNFVGGFSALVQKGFSAGDRLLIEAIPEALAKTDLVCSSVNVGSTKAGINMD
                     AVALMGPIVKRAAEITAQRSCIGPAKLVVFCNAPEDNPFMAGAFHGPGEPDCEIHVGV
                     SGPGAVRAALAKQAEGMDLGQVAELVKRTAFKITRMGQLVGNLVSERLGVPFGIVDLS
                     LAPTPAAGDSVANVLEEMGLESCGCCGTTAALALLNDAVKKGGVMASNFVGGLSGAFI
                     PVSEDAGMIAAARCGSLSIEKLEAMTAVCSVGIDMVIIPGDTAPEVISALIADEAAIG
                     MVNSKTTAVRVIPAIGKQVGDTLDFGGLLGWGPVMEINRFSPQKFVSRGGRIPAPLQS
                     LKN"
BASE COUNT          804 a         1495 c         1379 g          810 t
ORIGIN      
        1 cgatgaccag cgccggacgc accgcgccgc aaaggtcgca cagcatctcg ccgaaccgct
       61 cccggtcggg gaaacgcatg tgcatctccg ccttttgcag gccggggatg cagccgaaca
      121 ggtttttcac cgcagccgtc aggccggtca tcacatgggt cttgaacttg ggcaggtcga
      181 tgatgaaatc gctctccagc accggggtga tgaggttgaa ctcgcgcacc agccgcccgc
      241 ccgaggcccg ggcgccgctg ccggttttac tccagacctc cacccccagg cgctcgcagg
      301 ccgcggcgag gccgctggcc ctatagatgc cgcgcatgac cccctcgctg tagaccccgc
      361 cgcaggagtc ggccagcacg atgtgctcca ccccgcggcg gcgcagcgcg gcgaccaccg
      421 cctccaccac cgccggatgg gtggtcacgg cgtgctccgg cgggtgcttt gccagcaggt
      481 tgggcttgag cagcacgcgg ctgcccggcc cgagccgccc ggcggccggg cagagccgaa
      541 agatctcctc caccgcctcg gccagcccct cgggcctgcc gtaggacagc cccgtcctgt
      601 ggtagaccca atgctccggc tcagccatgg ccgcactcct ttaaaaactc ctcgatacgg
      661 cccagcgcct ctgagaggtg agcgaccgaa taggagtaag agatgcgcac aaagccctcg
      721 ccggaatcgc cgaacgcggt gccgggcacc acggccacct tttttgagaa gagcagcttt
      781 tcgcaaaact cctccgacct caggccgctg accgcgatgc tggggaagac gtagaacgcg
      841 ccctccggct caaagcaggt catgcccatg cggttgaggg cgctcaccaa aaacttgcga
      901 cgcatgtcgt actcgtcccg catggcctcg atggcgtcgt ccccctcccg cagggccaca
      961 atgcccgcgt actggctggt ggtgggggcg cacatgatgg cgtactggtg gatcttggtc
     1021 atggctttca tcagcggggc gggcgcgcag acatagccca gccgccagcc ggtcatggca
     1081 taggccttgg agaagccgtt gatatagatg gtgcgctctt tcatgcccgg cagcgaggcg
     1141 atgctcacat ggcgccgggc gccgtaggtc agctcggcgt agatctcgtc ggtgagcacc
     1201 aaaatgtcgg tgtcctgcag cacctcggcc agggcggcca gatgccgctc ccgcagcacg
     1261 gcgccggtgg ggttgcaggg gaaaggcagc accaaaatct tggtgcgcgg ggtgatcttt
     1321 gcccgcagct cctccgccgt cagccgaaag ccgttttccg ctttcagcgg caccggcacc
     1381 ggcacgccgc ccacaagcga ggtcagcggc gcgtagctga catagcaggg ctcggggatg
     1441 atgacctcgt cccccggctc cagcaccgcg cgcagcgcca ggtcgatggc ctcgctgcct
     1501 cccaccgtga ccagcatctc gctctgcgga tcgtagtgca gctcaaagcg gcgctccaga
     1561 taagcggcga tctcggcccg catctccgcc aggccggcgt tgctggtgta ccaggtcttg
     1621 ccccgctcca ggctcttgat gcccgcgtcg cggatcatcc agggcgtctt aaaatccggc
     1681 tcgcccaccc ccagcgagat acagccctcc atggtgcccg ccacatcaaa aaacttgcgg
     1741 atgccggagg gtttcagggc ctgcgcccgg gcgctcaaca gtttgctata ctctatcaca
     1801 ggaagttgta ccctctttca tccctatttt catcggtgta catcaccccg tcccgcttat
     1861 aggtgcgcag cacgaaatgg gtggcggtgg acagcacgtc gtccagcggc gagagccgca
     1921 gcgccacgaa cagggcgatc tcctgaaagc ttttgccggt caggatgagc cccaggtcat
     1981 agccgccgct catcagcata acgctgtcca cctcggggaa gagcgcgatg gtggaggcga
     2041 tctcgtcaaa gccgtggtcc tttttggggc tcaccttgag ctcgatcagc gcccgcaccc
     2101 ggaccgaatc cgtcttgtcc cagtccacca gggtcttata gcccttgatg atgccggact
     2161 ttgcatattc atcgatcgcc gccgcgacct cctccggggt cttgtccacc atcgccgcga
     2221 tatcctccag cgggagacgc gcgttgtcct ctaaaatctt cagaatacgg tccatactgt
     2281 cccacccttt ccctcttgat ccttttttct ccctgccctt gggcgagagg agaatttaac
     2341 tcattatact tgcaaagccg gcctttgaca agggctgccc cggtgcttgc ccattgagcg
     2401 cgcgcgcgtt atctggtata ataaaaaata atcgcaggct attttttcgg ccatccgttt
     2461 cggttgccgc gacagcggcg agaaacgggg ccatcaagat cagttgtttt tcagccacgc
     2521 ggcccctctt tggggccctg gccataaaga taagtagttt tttatacaaa gagaggggta
     2581 cacatatgaa agctgtcgta accgtcgtcg gccgcgatac cgtgggcatt ctggccaaga
     2641 tcgccggtgt ctgcgccgag accaacgtca acgtggagga ggtcacccag agtattctgt
     2701 cgggcacctt tgccatgatc atggtggtgg acgtcaccgc ctgcaccatt gattttgccg
     2761 agctgcagcg ccgctttgcc gccgcgggcg aagaaaaggg cgtggacgtc aaggtgaccc
     2821 gccaggagct ctacgacgcc atgcaccaca tctgacgcgg aaagggagga taaaaccgcc
     2881 aatgctggat acaaaggata ttttggagac catccgcatg atcagcgacg agaacctgga
     2941 cgtgcgcacc gtcacgatgg gcatctcgct gctggactgc gcggatgagg acccgcacag
     3001 ggcctgtgaa aagatttacc gcaagatcac cgaccgcgcc cgggacctgg tgcgcaccgc
     3061 cgaggaggtc agcgccgcct atggggtgcc catcgtcaac aagcgcatct cggtgacccc
     3121 catcgccatg ctgctggcaa gctgcaagga cccggacccg gtggagttcg ccatgacgct
     3181 ggacgccgcc gccaaaaagg tgggggtcaa ctttgtgggc ggcttttccg ccctggtgca
     3241 aaagggcttt tccgccggcg accgcctgtt gatcgaagcg atccccgagg cgctggcaaa
     3301 gaccgacctt gtctgcagca gcgtcaacgt gggctccacc aaggcgggca tcaacatgga
     3361 cgccgtggcc ctgatgggcc ccatcgtcaa acgggccgcc gagatcacgg cgcagcgcag
     3421 ctgcatcggc ccggccaagc tggtggtctt ttgcaacgcg cccgaggaca acccctttat
     3481 ggccggcgct tttcacggcc ccggcgagcc ggactgcgag atccacgtag gcgtttccgg
     3541 ccccggcgcg gtgcgcgccg cgctggcaaa gcaggccgag ggcatggacc tgggccaggt
     3601 ggccgagctg gtcaaacgca ccgctttcaa gatcacccgc atggggcagc tggtgggcaa
     3661 cctggtgagc gagcggctgg gggtgccttt cggcatcgtc gacctgtccc tggcccccac
     3721 ccccgccgcg ggcgattcgg tggccaatgt gctggaggag atgggccttg agagctgcgg
     3781 ctgctgcggc accaccgccg cgctggccct gctcaacgac gcagtcaaaa agggcggcgt
     3841 gatggcctcc aactttgtcg gcggcctctc gggcgctttt atcccggtct cagaggacgc
     3901 cgggatgatc gccgccgccc gctgcggcag cctttccatc gaaaagctgg aggccatgac
     3961 cgccgtgtgc agcgtgggca tcgacatggt catcatcccc ggcgacaccg cgccggaggt
     4021 gatctccgcc ctgatcgccg acgaggcggc catcggcatg gtcaactcga agaccaccgc
     4081 cgtgcgggtc atcccggcca tcggcaagca ggtgggcgac acgctggact tcggcggcct
     4141 gctgggctgg ggcccggtga tggagatcaa ccgcttctcg ccgcaaaaat tcgtctcccg
     4201 cggcggccgc atccccgcgc cgctgcagag cctgaaaaac tgaatccccg tcgtatgtaa
     4261 aaaagcaggc ccgccgatac tttgtaccgg agggcctgct tttctctgtc tttctataat
     4321 cctcgcaagc cgtctcaaaa acggcttttt cacgcgcttc tcttttccgc cgccgcgcgg
     4381 catcgggcag gggtgaaaaa acagtcttgc ccctcagccc gtccgctcat acgcgccggg
     4441 ctcccttttg tcggggccgc ggcctgtcgg cctgtgcgcg caggggcc
//
LOCUS       DBMDYA010000156         4444 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4705_length_4444_cov_1.429196, whole genome shotgun sequence.
ACCESSION   DBMDYA010000156 DBMDYA010000000
VERSION     DBMDYA010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4705_length_4444_cov_1.429196"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..825
                     /locus_tag="ACZPFK_05120"
     CDS             28..825
                     /locus_tag="ACZPFK_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1405723.1"
                     /translation="MKNNRGAIQKRQMQLLHYLKEKGFADVTQLSELLRVSPITVRRD
                     LDELAGRGLVTRYFGGAKLAESAQEDDEPAYLETTTQHLAQKRAIARRAAEMLENGDT
                     VFINSSSTALLIYPYIQDRSVLVVTNNGRSLLSQRAAGVELVLTGGEVYGDKQSLVGQ
                     FALDALSRITATKCILGVSGISVQGGITSRVIQETAINQMMLRRCSGPKIVVADYTKI
                     GLEHNFFSGRLGDITHLITDTGADRQKLDSIRCAGITVDAVEPLEDS"
     gene            1649..2092
                     /locus_tag="ACZPFK_05125"
     CDS             1649..2092
                     /locus_tag="ACZPFK_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR1405724.1"
                     /translation="MKIRISEEPGCEETEVLVRCREMDEEVLRLLARLRVLDAKLTGE
                     RNGATCLLDAGQVLYADTADKRTFLYTAEGVYETALRLYELEEQLAARDFIRAGKSVL
                     LNFGHVTSLRPDFGGRMLVTLSNGEQIMASRQYVPAIKAKLGIKK"
     gene            2150..2623
                     /locus_tag="ACZPFK_05130"
     CDS             2150..2623
                     /locus_tag="ACZPFK_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405725.1"
                     /translation="MKKWLEFLIPVKTAACYSFTAALFIYTVICLFTGRASIPIGIVL
                     SFLLLSLAAAVLQIVAFTSLVFKKLRYGSRLLLFILPFFAVLSLIAKGFHWFPPELGS
                     AWMLFLLLFLLIFVVMTVGFEIYFRVTGHKYDGLLGQYRLAKRKGADCPPPPSQL"
     gene            complement(2864..>4444)
                     /locus_tag="ACZPFK_05135"
     CDS             complement(2864..>4444)
                     /locus_tag="ACZPFK_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1405726.1"
                     /translation="NAQNANAAKQNFLSRMSHEIRTPMNAIIGMTTIAGANIEDRQRV
                     ENCLEKIGYSSKHLMTLINDVLDMSKIDEGKMTIAHEPFDLERVAESVTSIFYPQTVE
                     KGVAFSMPLIDITETALIGDSLRLNQVLINLLSNALKFTPPGGAVRLKIEQLQKKNER
                     VRLRFAVSDTGIGMSEEFMTRLFLPFEQESGATSQKYGGTGLGLSITKNLVTLMGGVI
                     SVKSEVGKGSTFTVELDFDVDGAPATRPAHDPQALEALKVLIADDDQDCCTHTSLLLK
                     KMGIRSHWVLRGIECVKEVIAAHKTGEDYDVCLIDWRMPDMDGIEVTRRVREVVGPDT
                     TIIIITAYDWTSIEKSAREAGANAFLFKPVFASALYNALLSVTGAAEPAQKQEDNKEK
                     SALAGRRVLLAEDNDLNREIAVELLKMVGMEIDCARDGQEAVEQFLQEGDAYDLILMD
                     VQMPVMNGYQATRAIRSSEHPKAKSIPIIAMTADAFHEDIVKADEAGMNGHLSKPIDP
                     DRLYQMMAFFVVAPLPAS"
BASE COUNT          851 a         1350 c         1353 g          890 t
ORIGIN      
        1 gtcgcaacag ttgcgaggag aaaaagcatg aaaaacaaca gaggcgccat ccagaagcgg
       61 cagatgcagc tgctgcacta tctcaaggaa aagggctttg cggatgtgac ccagctctca
      121 gagctgctgc gggtctcccc catcacggtg cggcgggacc tggacgagct ggccgggcgc
      181 gggctggtga cccgctactt cggcggggcc aagctggctg agagcgcgca ggaggacgac
      241 gagcccgcct atctggaaac gacaacccag catctggccc aaaagcgcgc catcgcccgg
      301 cgggcggccg aaatgctgga aaacggcgac accgttttta tcaactccag ctccacagct
      361 ttgctgatct atccttacat acaggaccgc tcggtgctgg tggtcaccaa caacggccgc
      421 agcctgctgt cgcagcgcgc cgccggcgtc gagctggtgc tcacgggcgg cgaggtgtac
      481 ggcgacaagc aatcgctggt gggccagttc gcactggacg cgctgtcccg catcacggcg
      541 accaagtgca ttctgggcgt cagcggcatc agcgtacagg ggggcatcac cagccgggtg
      601 atccaggaga ccgcgatcaa ccagatgatg ctgcggcgct gcagcggccc caagatcgtg
      661 gtggcggact acaccaaaat cggcctggag cacaactttt tcagcggtcg gctgggggac
      721 atcacccatc tgatcaccga caccggggcc gaccggcaaa agctggacag catccgctgc
      781 gcgggcatca ccgtggacgc ggtggaaccg ctggaggaca gctgaaagcg gacggcccaa
      841 ggccgcattt ccacagtaaa gccctgcccc cgaagaggga aagccgcgat ggggagcggg
      901 aaatttttcg cgccgccccc ccgcggcgat gtccgcctgc ccgcgcaggc gttgcgcaag
      961 gcaaagccgc aggttccgct gcggccgcgg cgtgacgacg gctgcgatgc tgcggctgcg
     1021 gagagaattg gacggcgcac ggcgcacaaa gcgctctccc ggggcgcgcg gcgaaagcgg
     1081 cggtttcgtg agcgggcacc atgcaaaaaa ggtggacaat ccccgtggat tgtccacctt
     1141 ttttacatcc gcttttgcgt gtaccggggc gtcaaaacgt ccgtcccaaa ccactgtttc
     1201 ccctctacca tgcgcccaac tgaaaagcgt cgcaagaggg cctgtgacaa gccaagggcc
     1261 gcagaaaacg caggcgattt ttcggcggag gaaacaaaaa cacaggcaac tccttgtgac
     1321 ttaacgagta ttttggcgaa atatgccgca acaaagcgtc cctgttttcg agcagagctc
     1381 tcgtcaacgg ccccaaaggg cgtggagggc ggatctccgg caggggtttc attcacgacc
     1441 taaaacaggg ggcattgccc ggctggaaaa accggcgacg ccccctgccg tctgcctcct
     1501 ataaaaaagc ggcggcagct tctccctggc actcagctcc cctcggccgc aggttggaag
     1561 cgcttttgaa ccggtcgcgc caaaacgctt gccgcgccgc gccccggggg gtatgataaa
     1621 gccgtagcta cagaaaggcg ggatgcaggt gaagatccgc atcagtgaag agcccggctg
     1681 cgaagagacg gaggtgcttg tgcgctgccg ggagatggac gaggaggttc tgcggctgtt
     1741 ggcccggctg cgggtgctgg acgccaagct gaccggcgag cggaacgggg ccacctgtct
     1801 gctggacgcc gggcaggtgc tgtatgccga cactgcggac aagcgcacat tcctctatac
     1861 ggccgagggg gtctacgaga ccgctctgcg gctctacgag ctggaggagc agctcgccgc
     1921 ccgggatttc atccgggccg gcaagtcggt gctgctcaat tttgggcatg tgacctctct
     1981 gcgcccggac tttggcggca ggatgctggt caccctgtcc aacggggagc agatcatggc
     2041 ctcccggcaa tacgtgcccg ccatcaaggc caagctgggc atcaaaaaat aaaaacccat
     2101 cccatcgccg gggagcgcct ttgcgccccc ataaaaggag gtttttttcg tgaaaaaatg
     2161 gcttgaattt ctgatccccg tcaagacggc tgcctgttac agctttacgg cggccctctt
     2221 catctacacc gtgatctgtc tctttactgg ccgggcgagc atccccatcg gcatcgtgct
     2281 ctcctttctg ctgctctcgc tggcggcggc ggtgctgcag atcgtcgctt tcacctcttt
     2341 ggtcttcaaa aaactgcgct atgggtcccg gctgttgctg ttcattttgc cgttctttgc
     2401 cgtgctcagc ctgatcgcca aggggtttca ctggttcccg ccggagctgg gcagcgcctg
     2461 gatgctgttt ttgcttctct tcctgctcat ctttgtggtc atgaccgtcg gttttgagat
     2521 ctactttcgt gtcacagggc acaaatacga cggtctgctg ggccagtacc ggctcgccaa
     2581 gcggaaaggc gcggattgcc cccctccccc gtcgcagctg taaaccggcg acagcggccg
     2641 ggagcccaca gggctcggtt accggccgcc tgtcggcttc cgcgcacccg cggcggcgga
     2701 gagacatgtg caagcagcgg ccgagccggc cgacgcgctt tgaaacaaat ggagagcgga
     2761 ggaaccctat ttttaaaggt tcttccgctc tccgtttatt tggtgtcggg gcgttcctgg
     2821 ccggaacgac gccgccccca gggccgcccg ggcttttacc aactcacgag gccggcagcg
     2881 gagcgacgac aaaaaaagcc atcatctgat agagccggtc cgggtcgatg ggcttcgaga
     2941 gatgcccgtt catgcctgct tcgtccgctt ttacgatgtc ctcgtgaaaa gcgtcggcgg
     3001 tcatggcgat gatagggatg ctctttgcct tggggtgctc tgagctgcgg atcgccctgg
     3061 tcgcctgata gccgttcatg accggcatct ggacatccat caggatgaga tcgtaagcgt
     3121 ccccctcctg caggaactgc tccacggcct cctgcccgtc acgggcgcag tcgatctcca
     3181 tacccaccat ttttaagagc tccacggcga tttcccggtt caggtcattg tcctcggcca
     3241 gcagcacgcg gcggcccgcc agggcggatt tctccttatt gtcctcctgc ttttgggccg
     3301 gctctgcggc gccggtcaca gagagaagcg cattgtagag tgcagacgca aagacgggct
     3361 tgaacagaaa tgcgttggcg cccgcctccc gggcgctctt ctcgatcgag gtccagtcgt
     3421 aagccgtgat gatgatgatc gtggtgtcgg gcccgacgac ctcccgcacc ctcctggtga
     3481 cctcgatccc atccatatcc ggcattctcc agtcgatcag gcacacatcg tagtcttctc
     3541 ctgttttgtg ggccgctatg acctctttca cacactcgat ccctctcaag acccaatggg
     3601 accggatccc catttttttc aagagaagcg aggtgtgggt gcagcagtcc tggtcgtcgt
     3661 cagcgatcag caccttgagc gcttccaggg cctggggatc gtgcgcaggc cgggtcgcag
     3721 gcgcgccgtc cacatcaaaa tccagttcta cggtgaaagt gctccctttc cccacctcgc
     3781 tttttaccga aataacgcct cccatcaggg tgaccaggtt tttggtgatg gaaaggccca
     3841 gcccggtgcc gccgtatttt tggctggtgg cgccgctctc ctgttcaaag ggcaaaaaga
     3901 gccgcgtcat aaactcttcg ctcatgccga tgccggtgtc gctgaccgca aaccgcagcc
     3961 gcaccctttc attcttcttt tgcagctgct cgatctttag gcgcacggcg ccgccgggcg
     4021 gcgtgaattt gagcgcgttg gagagaagat tgatgagcac ttggttcagc cgcaaagaat
     4081 cgccgatcag agccgtctcg gtgatgtcga tcaagggcat ggaaaaggca acgcctttct
     4141 caacggtctg cggatagaaa atagaggtca ccgactcggc cacccgctcc agatcgaaag
     4201 gctcgtgggc gatggtcatt ttcccctcgt cgattttgga catgtcgagc acgtcgttga
     4261 tgagggtcat cagatgcttt gaggaataac cgatcttttc aaggcagttt tccacccgct
     4321 gccggtcttc tatatttgcg ccggcaatgg tcgtcatgcc gataatggcg ttcatggggg
     4381 tgcggatctc atggctcatg cgggagagga aattctgttt ggccgcattg gcgttttgcg
     4441 cgtt
//
LOCUS       DBMDYA010000157         4424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4732_length_4424_cov_1.847560, whole genome shotgun sequence.
ACCESSION   DBMDYA010000157 DBMDYA010000000
VERSION     DBMDYA010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_4424_cov_1.847560"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="ACZPFK_05140"
     CDS             complement(<1..539)
                     /locus_tag="ACZPFK_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405727.1"
                     /translation="MQPTEDILVLKKINKAFPGVKALTDMDFSLRRGEVHALVGENGA
                     GKSTMMKILSGAYKKDSGTITFEGKEVDITSPKFSEELGIAIIYQELNLIHRISVAEN
                     VFLGRPPRRAGVIQWGKMYRDAKALFDSFDIKMDVYEDVRKLSIAQQQIVEIIKAVSI
                     NAKVVIMDEPTSSLTENET"
     gene            complement(666..1688)
                     /locus_tag="ACZPFK_05145"
     CDS             complement(666..1688)
                     /locus_tag="ACZPFK_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1405728.1"
                     /translation="MKKLVSIILAAALAFSLAACGGGSDSSSSASQSASQAADSVASK
                     AEDGKALKIGFALPTLQFPFYVRMHDAFVEECEKRGWEVVFVDGNLDSLTEVNGCLDL
                     ISKNVDALVFATWWMDAMVDVFDLAAKSDIPVYMMDGVTTPDGVEYVSITGTDNYDAG
                     KVGGTWMAGQLKAEGKTSVNLINITMASESAVDRCNGFMDGMKENGIEVNLVNDYIGD
                     TRAKSMQAAEDALVTYGEGKVDLIFGYSAQSSLGAYDAVVAANRSEIKVMGFDGEQDE
                     LDLIDAGTQYVGTIIQYPDKMGVLTAELIEKTLIGGETVEQKQPMSAGVYCADGALSG
                     EEIMGK"
     gene            complement(1766..2161)
                     /gene="rbsD"
                     /locus_tag="ACZPFK_05150"
     CDS             complement(1766..2161)
                     /gene="rbsD"
                     /locus_tag="ACZPFK_05150"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIR1405729.1"
                     /translation="MKKDRILNPEITAAVAALGHTEYLCIGDCGLPVPAGVKTIDVSV
                     TAGLPRFLQVLEAVAGELVVESYICAGEIDEKNPDTLRRMEQILEGKPARKVPHEEFK
                     RLMEKAKCVIRTGETSPYANVILIGGVNF"
     gene            complement(2200..3189)
                     /locus_tag="ACZPFK_05155"
     CDS             complement(2200..3189)
                     /locus_tag="ACZPFK_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405730.1"
                     /translation="MTIKEVASIAGVSIATVSKVINGCDEHISEATREKVRAVVAKYN
                     YIPNSVARGLKTRKSKIIGILLPDITNPYFPQIVRGIEDAANLRGYGVVFCNTDDNPD
                     RELECYNFLRSKMVDGIIFTRSLKRSTMNKVLTEDLPAVVLDRVERDDRADKAYGKVY
                     VDVRRTMYDATMKLFQAGCGEIACLSAASDSKSDRFLGYLRALETLDLPYDKSHVFLD
                     KFTEDTGYRGVKAVLQAHPETDGLVCGNDLIAIGALQALSELGRAVPRQVKVIGIDNI
                     YFSNFTTPPLSTMGQPTYTMGESAASMLIDHLEYGKPLFECVFDHEYIERQTV"
     gene            complement(3329..4303)
                     /locus_tag="ACZPFK_05160"
     CDS             complement(3329..4303)
                     /locus_tag="ACZPFK_05160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR1405731.1"
                     /translation="MKADALWVVEPFKIEVRPFEVEEPKYDEVMFKTMACGLCCWDSS
                     LYQCITAPGPAPYVIGHEGVGIVEKVGEGVTNVKPGDKVFCASGSNVMMSEYTTVKSD
                     CVVKLPDDTTDWASAVIEPNCCVVNLLNTTNIQAGDHVVLVGAGYMGTMTLQGLTRGS
                     QAGRITVFELRQDRREMAKAYPGCEVYDPESEEGKKVIDEIVAKGGADVVIDFGASKS
                     GFELANKLFRQGGKLTLGSWHRGMMEFDGSRWHLGGVTVYNLSPMSNDHYTDMLPRTY
                     ELIKRGVYEPGKLVTHTVHFRDSEKLAEVFMHSIKKTDGYMKAVILFD"
BASE COUNT          764 a         1370 c         1327 g          963 t
ORIGIN      
        1 tgggtctcgt tttcggtcag cgaagaggtg ggctcgtcca tgatgacgac cttggcgttg
       61 atgctgaccg ccttgatgat ctcgacgatc tgctgctggg cgatgctcag cttgcgcaca
      121 tcctcataga cgtccatctt gatgtcgaag ctgtcgaaca gcgccttggc gtcccggtac
      181 atcttgcccc actggatcac ccccgcccgg cgcggcgggc ggccgagaaa aacgttttcc
      241 gccacgctga tccgatggat gaggttgagc tcctgataga tgatggcgat gcccagctcc
      301 tctgaaaatt tggggctggt gatgtccacc tctttgccct caaaggtgat ggtgccgctg
      361 tcttttttat aagcgccgga caggatcttc atcatagtgg atttgcccgc gccgttctcg
      421 cccaccaggg cgtggacctc gcccctgcgc agggaaaagt ccatatcggt cagcgccttg
      481 acgccgggga aagccttgtt tatctttttc agaaccaaaa tgtcttcggt cggctgcatg
      541 tctttcctcc attccgtcgc agcacgttgg gtttctcaaa agaagccctg cggagcacgg
      601 gcgggggatg cccccgcccg cgcccccgga cttccgctgt cgtgctgcgg tgcaaagacc
      661 ggtccttact tgcccatgat ctcctcgccg gaaagggcgc cgtccgcaca gtacacaccg
      721 gcggacatcg gctgcttctg ctccacggtc tcgccgccga tcagggtctt ctcgatgagc
      781 tcggcagtga gcacgcccat cttgtcgggg tactggatga tggtgcccac atactgggtg
      841 cccgcgtcga tcaggtccag ctcgtcctgc tcgccgtcaa agcccatcac cttgatctcg
      901 ctgcggttgg cggccacgac cgcgtcgtag gcccccaggc tgctctgtgc gctgtagccg
      961 aagatcaggt ccaccttgcc ctcgccgtag gtcaccaggg cgtcctcggc cgcctgcatg
     1021 gatttggctc gggtgtcgcc gatatagtcg ttgaccaggt tgacctcgat gccgttttct
     1081 ttcatgccgt ccataaagcc gttgcagcgg tccacggcgg actcgctggc catggtgatg
     1141 ttgatcaggt tgacgctggt cttgccctcg gccttgagct ggccggccat ccaggtgcca
     1201 cccaccttgc ccgcgtcgta gttgtcggtg ccggtgatcg agacatattc cacgccgtcg
     1261 ggggtggtga cgccgtccat catgtacacg gggatgtcgc tcttggcggc gaggtcgaac
     1321 acgtccacca tcgcgtccat ccaccaggtg gcgaacacca gcgcgtccac atttttgctg
     1381 attaggtcaa ggcagccgtt gacctcggtg agcgagtcca ggttgccgtc cacgaagacg
     1441 acctcccagc cgcgcttttc gcactcctcc acaaaggcgt cgtgcatgcg gacgtagaac
     1501 gggaactgca gggtgggcag ggcaaagccg atcttcagcg ccttgccgtc ctccgccttg
     1561 gaggcgaccg aatcggccgc ctgggacgcg gattgagagg cggaggagga gctgtcgctg
     1621 ccgccgccgc aggccgccag gctgaacgcc agggccgccg ccagaatgat gctgactaac
     1681 tttttcataa ttttcctcca aattcttttt tgggcgccgc cgcagccgcg gcggtccgcc
     1741 ctgatctcaa gacatcagat agagctcaaa aattcacgcc gccgatcagg atgacgttgg
     1801 cgtaggggga ggtctcgccg gtgcggatga cgcattttgc cttttccatc aggcgcttga
     1861 actcctcgtg gggcaccttg cgcgccggct tgccctccaa aatctgctcc atgcggcgca
     1921 gggtgtcggg gttcttctcg tcgatctcgc cggcgcagat atacgactcc accaccagct
     1981 cgccggccac ggcctccagc acctgcaaaa aacggggcag cccggcggtg accgacacgt
     2041 cgatcgtttt cacccccgcg ggcaccggca ggccgcagtc gccgatgcat aagtactcag
     2101 tgtgccccag cgccgccacg gcggcagtga tctccgggtt caaaattctg tcttttttca
     2161 taaagtgtgt tccatcctca aaaatttgat ctgcggcgct cagacggtct gccgctcgat
     2221 atactcatgg tcaaagacac attcaaagag cggcttgccg tactcgagat ggtcgatgag
     2281 catgctggcc gccgactcgc ccatggtgta ggtgggctgg cccatcgtgg acaggggcgg
     2341 cgtggtaaaa ttggaaaaat agatgttgtc gatgccgatc acctttacct gccgcggcac
     2401 cgcgcgcccc agctccgaga gggcctgcag ggcgccgatg gcgatcagat cgttgccgca
     2461 gacaaggccg tccgtctccg ggtgcgcctg cagcaccgct ttcaccccgc ggtagccggt
     2521 gtcctcggtg aacttgtcca aaaacacgtg gctcttgtca tagggcaggt ccagcgtctc
     2581 cagcgcccgc agatagccca gaaagcggtc gcttttgctg tccgaggcgg ccgacaggca
     2641 ggcgatctcg ccgcagcccg cctgaaacag cttcatggtg gcgtcgtaca tggtgcggcg
     2701 cacatccaca tagaccttgc cgtaggcctt gtcggcccgg tcgtcccgct ccacccggtc
     2761 cagcaccacg gcgggcaggt cctcggtgag caccttgttc atggtcgacc gcttgagcga
     2821 gcgggtgaag ataatgccgt ccaccatctt ggagcgcaga aaattgtagc actccagctc
     2881 gcggtcgggg ttgtcgtcgg tgttgcagaa gaccacgccg tacccgcgca ggttggcggc
     2941 gtcctcgatg ccgcgcacaa tctgcggaaa ataggggttg gtgatgtccg gcagcaggat
     3001 gccgatgatc ttgctcttgc gcgtcttcag cccccgcgcg acggagttgg ggatataatt
     3061 gtacttcgcg accacagccc gcaccttttc gcgggtggct tcgctgatgt gctcgtcaca
     3121 cccgttgatg acctttgaca ctgtggcgat ggaaacgccg gctatgctcg cgacctcctt
     3181 gatcgtcaaa cctgatccct cccaatgtgt tttttaggta aacgtttacc taatcttggt
     3241 tcccatccgg cccggggtgg gccggctgtc gccggctcac gtctaggcca tccggaaaac
     3301 acaatagagg aaccaaagag ggtaggggtc agtcgaacag gatgacggct ttcatgtagc
     3361 cgtcggtctt tttgatcgag tgcatgaaca cctcggccag cttttcactg tcgcggaaat
     3421 ggacggtgtg ggtgaccagc ttgcccggct cgtagacgcc gcgcttgatc agctcatagg
     3481 tgcggggcag catatcggtg tagtggtcgt tgctcatcgg cgagaggttg tagaccgtca
     3541 cgccgcccag gtgccagcgg ctgccgtcga actccatcat gccgcggtgc cagctgccca
     3601 gcgtcagctt gccgccctgg cggaaaagct tgttggccag ctcgaagccg gatttggagg
     3661 cgccgaaatc aatgaccacg tcggcgccgc cctttgcgac gatctcgtcg atgacctttt
     3721 tgccctcttc gctctcgggg tcatagacct cgcagccggg gtatgccttt gccatctcgc
     3781 ggcggtcctg ccgcagctcg aacacggtga tgcggcccgc ctgtgagccg cgggtcaggc
     3841 cctgcagggt catggtgccc atgtagcccg cgcccaccag cacaacatgg tcgcccgcct
     3901 ggatgttggt ggtgttgagc aggttgacca cacagcagtt gggctcgatg acggccgaag
     3961 cccagtcggt ggtgtcgtcc ggcagcttga ccacacagtc ggatttgacg gtggtgtatt
     4021 cgctcatcat cacattggag ccgctggcgc aaaaaacctt gtcgccgggc ttgacgttgg
     4081 tgacgccctc gcccaccttt tcgacaatac ccacaccctc atggccgatc acatagggcg
     4141 cggggccggg ggcggtgatg cactgataca ggctggaatc ccagcagcac aggccgcagg
     4201 ccatggtctt gaacatgacc tcatcgtatt ttggctcttc cacctcgaaa ggccggacct
     4261 cgatcttaaa gggttccact acccaaaggg catctgcttt catggttctc gtacctccat
     4321 ttgtgtgttg cgttgtgtgt tgggccgggc ccggtcccgt gagtgaggct aattatatcc
     4381 gagctttttt gggatgtcaa acgattgatt ttccttgttt caag
//
LOCUS       DBMDYA010000158         4417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4740_length_4417_cov_1.761353, whole genome shotgun sequence.
ACCESSION   DBMDYA010000158 DBMDYA010000000
VERSION     DBMDYA010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_4417_cov_1.761353"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(210..2432)
                     /gene="ppk1"
                     /locus_tag="ACZPFK_05165"
     CDS             complement(210..2432)
                     /gene="ppk1"
                     /locus_tag="ACZPFK_05165"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR1405732.1"
                     /translation="MSGAEGKNAIFINRELSWLEFDRRVLELAKDKGVPLAEQLNFAS
                     IYASNLDEFFMIRVGSLYDQTLLKEEKKENKTNMTPAAQLRAIMPVAGELQEHCDKIM
                     AKLFSHLADYGYEKVDFSKLTKDEEHFWKKYFLSELYPVLSPQIIDRRHPFPFLRNLE
                     IYIGARVKEKDDGTEAFGLIPVSSQFERLICMKINGVTKFALVEELVRHFAHLAFGRY
                     TVAEGCIFRVTRNADINLQEAMQDQDLDYRAVMSELLKKRRKLAAVRLQITPRASDEI
                     VDFLCRKLVLDRRQSFAQSAPLDMGFARQLTGRMSKEGLTQLFYTPVRPMLPVPEFSL
                     TKAVRKRDVLIAYPFQSIRPFINMLNEAAVDPTVISIKMTLYRMATDSKIVEALINAA
                     ENGKEVVTIVELRARFDEQNNIDWSKQLESAGCTVIYGFEDYKVHSKLTLITRKVGGQ
                     YQYISQIGTGNYNEKTAELYTDLCFVSADQALGEEVAEVFNDLAVERTTAQTNRLLVA
                     PCRFKSVILEEIQREIDAKRAGQEAAITLKCNSISDRDLILKLSEASCVGVPVKMIIR
                     GICCFRAGVPELTENIEVRSIVGRYLEHSRIYSFGGTQNRRVYIASGDFLTRNTERRV
                     EIGVKIEDPAIKNTLVNLLRYQLTDNVNARQMQPDGTYVKVKRAEDEATVDSQMEMYN
                     YFANTWTDYYTSYTAPRRLAGAAEQLRERRAAEAKEKRSSLLQRLRGALGGGKKGEKR
                     "
     gene            complement(2455..2805)
                     /locus_tag="ACZPFK_05170"
     CDS             complement(2455..2805)
                     /locus_tag="ACZPFK_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR1405733.1"
                     /translation="MSAEGRRGAAKTALLFAALTAVLAAAALAYYPPLWGGGLRPAAE
                     SGFLETVRGDRLDLNTATEAELRALPGIGEAKAAAIVAYREAHGGFSSVEELLQVKGI
                     GEKILAGLRDHVCI"
     gene            complement(2795..3616)
                     /locus_tag="ACZPFK_05175"
     CDS             complement(2795..3616)
                     /locus_tag="ACZPFK_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR1405734.1"
                     /translation="MTKSLGDLLVVSDLDGTLLTSSEGLPGCNAAMLRLFSALGGRFT
                     VATGRSPSSARRALDGLPLGAPAIVFNGGMLYDYEKGVVAAEHFLPRQTALDAIRALT
                     AQAPEVGVEVLADNGRMYLPACGAVVQRHAVREGLPYVAAQLEDIPGRWYKVLFGAPP
                     AVLSSLEQFAAGLEWEGAYFTATSPTYLELMPQGVDKGSALRELCALIGVAAENVIAV
                     GDYYNDIGMLRAAGHPVAVGNAAPQVKLLCEGEVLPCRDGGVAQLLYELIRRYER"
     gene            complement(3613..>4417)
                     /locus_tag="ACZPFK_05180"
     CDS             complement(3613..>4417)
                     /locus_tag="ACZPFK_05180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1405735.1"
                     /translation="PSPAGAAGCRLPRLRAGDEVVCTLPPPRETAVLPQDIPLEIVYE
                     DGDLLVVNKPKGMVVHPAPGNYENTLVNALLFHCRDSLSGVGGELRPGIVHRIDKDTS
                     GLLVVAKNDAAHQGLAAQLAVHSMARVYQAVVYGRLGQDAGAIDAPIGRSGKDRKKMA
                     VTEKNSKRAVTAYRVLGRYRGFTHIELKLQTGRTHQIRVHMASLGHPVAGDPVYGPKA
                     VIRELEGQCLHAGVLGFVHPRTGRYLEFTAPLPEYFTNFLHKLQREESI"
BASE COUNT          771 a         1460 c         1345 g          841 t
ORIGIN      
        1 aatcgtcgaa aaaagggcgc gcgccctaaa acctccctcc ggacggtacc cccggaaagc
       61 cggcaaaggc aaggcgcgca ggaaagctcc ccgcgcgcct tatctttatc tcccgtcttt
      121 ttgccgccgc ggccggagcg cggccgtact gcacgatgcc agaggcttat cggcgctgat
      181 ctccagctga ggcgcagcgg aaaaacgcct tatctctttt cgcctttctt cccgccgccc
      241 agcgcgccac gcagccgctg cagcagcgag gaccgctttt cttttgcctc ggcggcgcgc
      301 cgctcgcgca gctgctccgc cgcgccggcc aaccggcgcg gcgcggtgta agaggtataa
      361 taatcggtcc aggtgttggc aaagtagttg tacatctcca tctggctgtc caccgtggcc
      421 tcgtcctcgg cgcgtttcac ctttacatag gtgccgtccg gctgcatctg gcgggcattg
      481 acattgtccg tcagctgata gcgcagcagg ttgaccaggg tgtttttgat ggccgggtcc
      541 tctatcttga cgccgatctc cacccgccgc tcggtgttgc gggtcagaaa atcgccggat
      601 gcgatgtaga cgcggcggtt ttgcgtgccg ccgaaagaat agatgcggct gtgctccaga
      661 tagcggccga cgatgctgcg cacctcgatg ttctcggtca gctcgggcac cccggcccgg
      721 aagcagcaga tgccgcggat gatcatcttc accggcacgc ccacacagct ggcctccgac
      781 agctttagga tgaggtcgcg gtcgctgatg gagttgcact tcaaggtgat ggcggcctcc
      841 tgcccggcgc gctttgcgtc gatctcgcgc tggatctcct ccaggatcac cgatttaaag
      901 cggcagggcg ccaccaacag acggttggtc tgtgcggtgg tgcgctccac cgccagatcg
      961 ttgaacacct ccgccacctc ttcgcccagg gcctggtccg cgctgacgaa gcagaggtcg
     1021 gtgtagagct ccgccgtctt ttcgttgtag ttgccagtgc cgatctgaga gatatactgg
     1081 tactgcccgc ccaccttgcg ggtgatgagg gtgagctttg agtggacctt gtaatcctcg
     1141 aagccgtaga tgacggtaca gcccgcgctc tccagctgct tggaccagtc gatattgttc
     1201 tgctcgtcaa agcgggcccg cagctcgacg atggtgacca cctctttgcc gttctccgcg
     1261 gcattgatca gggcctccac gatcttcgaa tcggtggcca tgcgatagag ggtcatcttg
     1321 atcgagatga cggtgggatc caccgccgcc tcattgagca tgttgataaa ggggcggata
     1381 ctctggaaag gataggcgat cagcacgtcc cgcttgcgca ccgccttggt gaggctgaac
     1441 tccggcaccg gcagcatggg acgcaccggc gtgtaaaaga gctgggtcag cccctccttg
     1501 gacatccggc ctgtcagctg gcgcgcaaag cccatgtcca gcggcgcgga ctgcgcaaag
     1561 ctctggcgcc ggtccagcac cagcttgcgg cagagaaaat ccacgatctc atccgacgcg
     1621 cgcggggtga tctgcagccg caccgccgcc agcttgcgcc gctttttcag cagctcgctc
     1681 atcaccgccc ggtagtcgag gtcctggtcc tgcatggctt cctgcagatt aatgtcggca
     1741 ttgcgggtga cccggaagat gcatccctcg gccacggtat agcgcccgaa cgccaggtgg
     1801 gcgaaatggc gcaccaactc ctccaccagc gcaaatttgg tcacgccgtt tatcttcatg
     1861 cagatcagcc gctcgaactg gctgctcacc gggatgaggc cgaaagcctc ggtgccgtcg
     1921 tctttctcct ttacccgcgc gccgatatag atctccagat tgcgcaggaa cggaaaggga
     1981 tggcggcggt cgatgatctg gggcgagagc accgggtaga gctctgagag aaaatacttc
     2041 ttccaaaaat gctcctcgtc cttggtgagc ttggaaaaat ccactttttc atagccgtag
     2101 tccgccagat gggaaaacag ctttgccatg atcttgtcac agtgctcctg cagctcgccg
     2161 gcgaccggca tgatcgcgcg cagctgggcg gcgggggtca tgttggtctt gttctctttc
     2221 ttttcctctt tcaaaagggt ctggtcgtaa agggagccca cccggatcat gaaaaattca
     2281 tccaggttgc tggcgtagat ggaggcgaag ttgagctgtt ccgccaaggg cacccccttg
     2341 tcctttgcca gctccagcac tctgcggtca aattccagcc agctgagctc ccggttgata
     2401 aagatcgcat tttttccttc tgcgccgctc ataaaaacta ctccattcct tttttcagat
     2461 acagacgtga tctctcagac ccgcaaggat cttttctcca atacccttga cctgcagcag
     2521 ctcttccaca gaggaaaagc cgccgtgcgc ttcgcggtag gccacgatgg ccgcggcctt
     2581 tgcctcgccg atgccgggca gggcgcgcag ctccgcctcg gtggcggtgt tgaggtcgag
     2641 ccggtcgccc cgcaccgtct ccaaaaagcc gctttccgcc gcgggccgca ggccgccgcc
     2701 ccacagcggc gggtaatagg ccaaagccgc ggccgccaaa accgcagtca gcgccgcaaa
     2761 cagcagcgcc gtcttcgcgg cgccccgccg cccctcagcg ctcatagcgc cggatcagct
     2821 catagagcag ctgcgccacc ccgccgtccc ggcagggcag cacctcgccc tcgcacagca
     2881 gcttcacctg cggcgccgcg ttgccgaccg ccaccgggtg acccgccgcc cgcagcatgc
     2941 cgatgtcatt gtagtagtcc cccaccgcaa tcacgttttc agccgcgacc ccgatgaggg
     3001 cgcagagctc gcgcagggcc gagcccttgt cgaccccctg gggcatcagc tcaagatagg
     3061 tggggctggt ggcggtgaag taagcgccct cccactccag cccggcggca aactgctcca
     3121 ggctgctgag aacagcgggc ggcgccccga acagaacctt gtaccaccgg ccggggatat
     3181 cctccagctg ggccgccaca taggggaggc cctcgcggac ggcgtgccgc tgcacgaccg
     3241 cgccgcaggc cggcagatac atgcggccgt tgtccgcgag cacctctacc cccacctccg
     3301 gcgcctgtgc cgtcagggcg cggatcgcat ccagcgccgt ctgccggggc aaaaagtgct
     3361 cggcagccac aacgcccttt tcataatcat atagcatccc gccgttaaac acaatggccg
     3421 gcgcgcccag cggcaggccg tccagcgccc gccgggccga ggaggggctg cgcccggtgg
     3481 cgacggtaaa gcggccgccc agggcgctga acaggcggag catggccgca ttgcagcccg
     3541 gcaggccctc cgaggaggtg agcagggtgc cgtccagatc gctgacgacg agcaggtccc
     3601 caaggctttt ggtcatatgc tttcttccct ttgcagttta tgtaaaaaat tcgtaaaata
     3661 ctccggcagc ggggcggtga actcaaggta tcgcccggtg cggggatgca caaagcccag
     3721 taccccggca tggaggcatt gaccctccag ctcgcggatc acggctttgg gcccgtagac
     3781 ggggtcgcca gccaccggat ggcccaggct cgccatatgc accctgattt ggtgggtgcg
     3841 gccggtctgc agcttcagct cgatatgggt aaagcctcta tagcggccga ggacacgata
     3901 ggccgtgacc gcccgcttgg aatttttttc ggtgacggcc atctttttgc ggtctttccc
     3961 gctgcgcccg atgggggcgt cgatcgcgcc cgcgtcctgc cccagccgcc cgtagaccac
     4021 ggcctgatac acccgcgcca tgctgtgtac ggccagctgc gccgccagcc cctggtgggc
     4081 ggcatcgttt ttggccacca ccagcaggcc gctggtgtcc ttgtcgatgc ggtggacgat
     4141 gcccggccgc agctctccgc ccacgccgga aaggctgtcc cggcagtgaa agagcagcgc
     4201 gttgacgagg gtgttttcat agtttccggg cgcggggtgg accaccattc ccttgggctt
     4261 gttgacgacc agcaggtcgc cgtcctcata gacgatctcc agcgggatat cctgcggcag
     4321 gaccgccgtc tcccgcgggg gcggcagggt gcagactacc tcgtctccgg cgcgcagacg
     4381 aggtagtctg caccctgccg cccccgcggg agacggc
//
LOCUS       DBMDYA010000159         4413 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4745_length_4413_cov_1.404453, whole genome shotgun sequence.
ACCESSION   DBMDYA010000159 DBMDYA010000000
VERSION     DBMDYA010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4745_length_4413_cov_1.404453"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2290)
                     /locus_tag="ACZPFK_05185"
     CDS             complement(<1..2290)
                     /locus_tag="ACZPFK_05185"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF014763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1405736.1"
                     /translation="MQAEKKTILKTILATVLAAALVMVLGCGYLFSVLKEQEKQSDRY
                     LLEVARQSAAVIEKQIEGDFNSLEAVARAIGESEEDFESLLPRLFRENFNNHFRRMAM
                     IAPDGTVYAVSYDAVSAGERNVRDEPFFRHAMKGERYLDDTRPDPLIEEAYINTYAVP
                     IYRDSDPTGTPMGVLTAVFNTDIFRDALGGSSFEGAGFAHIVKADGTFLMRSTNKNAD
                     QTVLNAFDDASRFESAGEMRRVQEDMAAGQSGTFAHTTPTGLEALVTYTPIGVQNWYL
                     FSVVPRSIVEGQTRQFTRTTVELLLVLSALFALALAFIMRVRQKSRQEAQRLAYVDPV
                     TGLYNRNRFRLEATRRLERQAGPPMAVMILNLSGFRAVNELFGFTEGDRLLAAVGGML
                     KEELGGDDLYARGEADNFYLLTGRYSPEGLRGLGEALAARVEREAQRQGQQFVLSARC
                     GVYLLTEADAGVREISVLLDRAKTALDAGRGEKERISFYNVALHEREQLRRAVEANLD
                     AALATHEFVVYLQPQFSAAGGGVTGAEALVRWDDPRRGLLSPGDFIPVLEENGSITGL
                     DFYVLEEVCRFLREWLDAGFDPVPVSVNQSRLHLYRADYLQRLLGTVHRYGVPPQLIV
                     LEITESAALDNAGALIKVTRELHAAGFRVSMDDFGSGYSSLNLLDSIPFDELKLDKRF
                     FDDFAGRSRGRTIVASILSMAKGLGISTVAEGIERKEQLEWLRREGCDAIQGFYLARP
                     MPRAEFERQVFGAESGERVKRVR"
     gene            2549..3313
                     /locus_tag="ACZPFK_05190"
     CDS             2549..3313
                     /locus_tag="ACZPFK_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405737.1"
                     /translation="MDKERFDALCLQEQAADGGIGTLGEKRLHRVLKVYFEPRPACRE
                     QPLAGYVADILNEEGVTEVQTRNYGALRKKLAAFLPLMPVRVVCPLPARKWVSWVDPA
                     TGEVSRRHPSPKRGAPIDACYELVRIAPLLAHPNLTLHLLFFDADEYRVRDGWDKSGK
                     RGSTRAELQPLGLCAEQEVRGPAAYADLLPAGLPDPFTLPELRAAARRSETLCRRAVY
                     TLEKAGALSRAGKRGRALLYRRTLPPEGRTPAAGRP"
     gene            3407..4246
                     /locus_tag="ACZPFK_05195"
     CDS             3407..4246
                     /locus_tag="ACZPFK_05195"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR1405738.1"
                     /translation="MKELRAEQITSAVRELCIEANYQLPPDVTACIRSCRERETWPVA
                     REILGNILDNVQAASGGDYPLCQDTGLACVFIRLGQDVHISGDLTAAVDEGVRQGYRD
                     GYLRASAVRDPLHRVNTGDNTPALLTVDLVPGESCEITVAPKGFGSENMGALKMLRPS
                     DGAAGVRQFVLDTVKAAGPNPCPPIVVGVGVGGSFDKVALCAKRALLRPLGQHHPDPA
                     WAAMEEELLEAINALGIGPQGFGGRTTALSVAIETMPTHITALPVAVNINCHVTRHAR
                     RVL"
     gene            4261..>4413
                     /locus_tag="ACZPFK_05200"
     CDS             4261..>4413
                     /locus_tag="ACZPFK_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017494.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1405739.1"
                     /translation="MTEITTPFTQEQADRLRAGDSVLFTGTLFTARDAAHARLCQALE
                     AGDPLPF"
BASE COUNT          756 a         1506 c         1418 g          733 t
ORIGIN      
        1 cacgcacccg cttcacccgt tcgccgctct cggcgccaaa gacctggcgc tcaaactccg
       61 cgcggggcat gggccgtgcc agataaaagc cctggatggc gtcgcagccc tcgcggcgca
      121 gccattccag ctgctctttg cgctcgatgc cctcggccac ggtgctgatg cccagccctt
      181 tcgccataga gaggatgctg gcgacgatgg tgcgcccccg cgagcggccg gcgaagtcgt
      241 caaaaaagcg cttgtccagc ttgagctcgt caaaggggat gctgtccagc aggttcaggg
      301 aggaataccc ggagccgaag tcgtccatcg acacccggaa gcccgcggcg tgcagctccc
      361 gcgtcacctt gatcagggcc ccggcgttgt ccagcgccgc gctctcggtg atctccagca
      421 cgatcagctg gggcggcacg ccgtagcggt gcaccgtgcc caacagccgc tgcagataat
      481 cagcccggta caggtgcaga cgcgactggt tcaccgagac cggcacgggg tcgaagcccg
      541 cgtccagcca ctcgcgcaaa aagcggcaga cctcctccag cacataaaaa tccagcccag
      601 tgatcgagcc gttctcctcc agcaccggga taaaatcccc aggggacagc aggccgcgcc
      661 gggggtcgtc ccagcgcacc agcgcctcgg cgccggtcac cccgccgccc gcagccgaga
      721 attggggctg cagatagacc acaaactcat gggtggccag cgccgcgtcc aggttggcct
      781 ccaccgcgcg gcgcagctgt tcccgctcgt gcagggccac attgtaaaag ctgatccgct
      841 ctttttcgcc ccgcccggcg tccagcgccg tcttggcgcg gtccagcagc acgctgatct
      901 cccgcacccc ggcatccgcc tcggtcagca ggtagacccc gcagcgggcg gagagcacga
      961 actgctgccc ctgccgctgg gcctcccgct cgacgcgggc ggccagcgct tcccccaggc
     1021 cgcgcagccc ctcggggctg taccgcccgg tgagaaggta gaagttgtcc gcctcccccc
     1081 gcgcgtagag atcgtcgccg cccagctctt ctttcagcat gccccccacc gccgccagca
     1141 gccggtcgcc ctcggtgaag ccgaacagct cgttgacggc gcgaaagccg ctcaggttga
     1201 ggatcatcac cgccatcggg ggcccggcct gccgctccag ccggcgggta gcctccagcc
     1261 ggaaccggtt ccggttgtac aggccggtga ccgggtccac atatgccagc cgctgggcct
     1321 cctggcggct cttttggcgc acccgcatga taaaggccag cgccagcgcg aacagcgccg
     1381 agagcaccag cagcagttcc actgtggtgc gggtgaactg gcgggtctgg ccctccacga
     1441 tcgagcgggg caccacgctg aacagatacc aattctgcac cccgatgggg gtataggtga
     1501 ccagcgcttc caggccggtg ggggtggtgt gggcaaaggt gccgctctgc cccgcggcca
     1561 tatcttcctg tacccgccgc atctccccgg cactctcgaa cctggaggcg tcgtcaaagg
     1621 cgtttaaaac agtctgatcc gcgtttttgt tggtgctgcg catcaaaaaa gtgccgtccg
     1681 ccttgacgat atgggcgaag cccgcgccct caaaagacga cccgcccagc gcgtcgcgga
     1741 agatatcggt gttgaacacc gcggtcagca cgcccatggg ggtgccggtg gggtcggagt
     1801 cccggtagat gggcaccgca taggtgttga tatacgcttc ctctatcagg gggtcggggc
     1861 gggtgtcgtc cagataccgt tcccctttca tggcatggcg gaaaaagggc tcgtcccgca
     1921 cgttgcgctc gccggcggag acggcgtcat agctcaccgc atacaccgtc ccatccgggg
     1981 ctatcatggc catgcggcgg aaatggttgt tgaagttttc gcggaagagc cggggcagca
     2041 ggctctcaaa atcctcctcg ctctcgccga tggcccgcgc caccgcctcc agcgagttga
     2101 aatcgccctc aatctgcttt tcgatcaccg cggcgctctg gcgtgccacc tccagcagat
     2161 agcggtcaga ctgcttttcc tgttctttca agacagagaa caggtaccca cagcccaaca
     2221 ccatcaccag cgcagctgcg agcaccgtgg cgaggatcgt ttttaggatc gtcttttttt
     2281 cagcctgcat ggcattctcc ccatcccgga aaagcagtca ctaaatatga tacatactta
     2341 taaatataag agtttttgtg acacatgtca acaaattttg ccctgcggag atcaaagtta
     2401 tcatttttga tgaaatatta tcttcacctt gtctgtttta ggacttctta ctttttgacc
     2461 tcttgctccg gcagatcgcc ccgccggggc cggtttgatt tttcccctct ttttctatag
     2521 aataggacaa aaagggggcg gcgagaggat ggacaaggag cggttcgacg cgctttgcct
     2581 gcaggagcag gcggcggacg gcggcatcgg caccctgggt gaaaaacggc tgcaccgggt
     2641 gctgaaagtg tactttgagc cccggccggc ctgccgggaa cagccgctcg ccgggtacgt
     2701 ggccgacatc ctcaacgagg agggggtcac cgaggtgcag acccgcaatt acggggcgct
     2761 gcgcaaaaag ctggccgcct ttctgccctt gatgccggtg cgggtggtct gcccgctgcc
     2821 ggcgcggaaa tgggtcagct gggtcgaccc cgccaccggc gaggtctccc ggcgccaccc
     2881 ttcccccaag cgcggcgcgc ccatcgatgc atgttatgag ctggtccgca tcgcgccgct
     2941 gctggcccac cccaatctga cgctgcacct gctgtttttt gacgcggatg agtaccgggt
     3001 gcgggacggc tgggacaaga gcggcaagcg cggctcgacc cgggccgagc tgcagcccct
     3061 gggcctttgc gccgaacagg aggtgcgcgg gcctgcggct tacgccgacc tgctgcccgc
     3121 gggcctgccg gacccgttca ccctgccgga gctgcgcgcg gccgcgcggc gcagcgaaac
     3181 gctctgccgc cgcgccgtct acacgctgga aaaagccggc gccctcagcc gggcgggcaa
     3241 gcgggggcgc gcgctgctgt accgccgcac cctgccgccg gaggggcgga cgcccgccgc
     3301 cggccgccct tgagctttgg gcgcgaatgt gatagcatgt cgataaaagt aaaaaagagt
     3361 tccggcgggc gggcagccgc cgcaccggcg aagggaagcg atcgtcttga aagaactgcg
     3421 cgccgaacag atcaccagcg ccgtgcggga gctgtgcatc gaggccaact atcagctgcc
     3481 gccggatgtg acggcctgca tccgctcctg ccgggagcgg gagacctggc cggtggcccg
     3541 ggagattttg ggcaatatcc tggacaatgt ccaggcggcc tcgggcgggg attaccccct
     3601 ctgccaggac accgggctgg cctgcgtttt catccgcctg ggacaggacg tgcacatcag
     3661 cggcgacctg acggcggccg tggacgaggg ggtgcggcag ggctatcggg acggttatct
     3721 gcgcgccagc gccgtgcgcg acccgctgca tcgtgtgaac accggcgaca acacccccgc
     3781 cctgctcacc gtggacctgg tgcccggcga gagctgcgag atcacggtgg cgcccaaggg
     3841 cttcggcagc gagaacatgg gggcgctgaa aatgctgcgc cccagcgacg gagcggcggg
     3901 ggtgcggcag ttcgtcctcg acaccgtcaa ggccgccggg cccaacccct gcccgcccat
     3961 cgtggtgggc gtgggggtgg gcggcagctt tgacaaggtg gccctctgcg ccaaacgcgc
     4021 cctgctgcgt ccgctggggc agcatcatcc ggaccccgcc tgggcggcga tggaagagga
     4081 gctgctggag gcgatcaacg ccttgggcat cggcccgcag ggcttcggcg gccgcaccac
     4141 cgcgctgtcg gtggccatcg agaccatgcc cacccacatc accgccctgc ccgtggcggt
     4201 gaacatcaac tgtcacgtca cccgccatgc gcggcgggtg ctgtaaagga ggcgcgcgcc
     4261 atgactgaga tcaccacccc gtttacgcag gagcaggccg accgcctgcg ggccggggac
     4321 agcgtgctgt tcaccggcac ccttttcacc gcccgggacg ccgctcacgc ccgcctctgc
     4381 caggcgctgg aggcggggga ccccctcccc ttt
//
LOCUS       DBMDYA010000160         4401 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4760_length_4401_cov_1.688372, whole genome shotgun sequence.
ACCESSION   DBMDYA010000160 DBMDYA010000000
VERSION     DBMDYA010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4760_length_4401_cov_1.688372"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="ACZPFK_05205"
     CDS             <1..1406
                     /locus_tag="ACZPFK_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1405740.1"
                     /translation="EVLKDLKDSEKAALTGEIDRVFEDFIDGTTGFLLRISSSRYLAV
                     MEERHLAEVVAGKFAVLDRMREIGNENGVVTLSVGVGRGGATFAECEKMARAALDMAL
                     GRGGDQAAVRSADGGFEFYGGVSRSVEKRTKVKSRIIANALGDIIRQSDSVIIMGHRL
                     SDLDAIGAAIGVLRICKIFDKPAAIAVDEKHTLAGSLIDRFRRSGLGEDFITPAEALE
                     GLTRRTLVIIVDTHVPQLLESGEIYRAANNVVVIDHHRKMVGHIDNAVIFYHEPYASS
                     TCELVAELLQYVGEREDRPTALESEAMLAGIMLDTRDFVINAGVRTFEAAAYLRRMGA
                     QTQNVKRLFAGSLESYTSKAKLVSEAVLYKGCAIVASDDVPTDSGVIVPQAANDLLSI
                     EGVQASFVAVDMGGFISVSARSLGEVNVQVIMEKLGGGGHLTMAGAQMKDVSLEKTKV
                     YIMAAIDEYRRNQAEEE"
     gene            1449..1892
                     /gene="rplI"
                     /locus_tag="ACZPFK_05210"
     CDS             1449..1892
                     /gene="rplI"
                     /locus_tag="ACZPFK_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1405741.1"
                     /translation="MKVVLKTDVKGTGKKDEMVEVSDGYARNFLLPRGLAVAANAAAV
                     NEVRTKESAKRHHAEVELADAQATAKKIGGMTVTVKAKAGQGGRLFGAVTGKDIAEAV
                     NKTAGVSIDKRKVVLDADIKNYGQYEVEIKVYPSVTAKVTVVVEE"
     gene            2273..3655
                     /gene="dnaB"
                     /locus_tag="ACZPFK_05215"
     CDS             2273..3655
                     /gene="dnaB"
                     /locus_tag="ACZPFK_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1405742.1"
                     /translation="MDNLSLTFENMGVQLPYNMEAEQAVLGAALLQGDCIPTLVEKLR
                     PEHFYVRQNGEIFAELVRLFTGGAAIDFVTVLGAVLDAGVFAGEEEAKVYLTGLAETV
                     PSISNVEHYAGIVYEKYLIRQLMGAAKDILEQTSEESDADILLESAEQKIYEIRSGRD
                     QSAMQPISSTIIDTYNQLQKISGPDRDKYLGIPTGFTYLDKMLTGLGRSDLIILAARP
                     GMGKTSFALNIATNVAKKQKIPVALFSLEMTKDQLTSRILSSEAAIDSQKLRTGTLSG
                     QDWVDLAQASDILAQAPIYMDDTSAITVPEIKAKVRRLNQDPAKPDVGLVVIDYLQLM
                     NSGKRTDSRVQEISDITRNLKIMAKELNVPIICLSQLSRNTERSSNRDHRPMLSDLRD
                     SGSIEQDADSVLFLYREAYYDTEGQSDTSTAECIVAKNRHGEVGTVSLGWDGAHTRFM
                     NIEYSHNDGY"
     gene            3642..>4401
                     /gene="tilS"
                     /locus_tag="ACZPFK_05220"
     CDS             3642..>4401
                     /gene="tilS"
                     /locus_tag="ACZPFK_05220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1405743.1"
                     /translation="MTDIEKLEKKAARWIEEQALLQSGDTVLCALSGGPDSVALFHAL
                     RRLAPALGVELRAAHLNHGLRGAEADRDEAFVRRLCARWSVPLFVERAGMALRPRPAG
                     QSEESFARALRYDFFERAAAAAQAQGCGPVKVATAHTASDNAETLLFRLARGTSLTGA
                     GGIPPRRGRYIRPLLDCTRAEVLAYCAASGWGYMIDSTNLAPCCSRNRLRLQAVPALR
                     EVNPDADAALSAFAADARETAAFLEGLARELLASA"
BASE COUNT          888 a         1318 c         1485 g          710 t
ORIGIN      
        1 acgaggtgct caaggatctg aaagacagcg aaaaggcggc tctcaccggc gagatcgatc
       61 gggtcttcga agattttatc gacggcacca ccggctttct gctgcgcatc tcctcctccc
      121 gctatctggc ggtgatggag gagcggcatc tggcggaggt ggtggccggc aagttcgccg
      181 ttctggaccg gatgcgcgag atcggcaacg agaacggcgt ggtgaccttg tccgtgggcg
      241 tgggccgcgg cggtgccacc tttgcagagt gcgaaaaaat ggcccgcgcg gcgctggata
      301 tggcgctggg ccgcggcggc gaccaggcgg cggtgcgcag cgcggacggg ggcttcgagt
      361 tttacggcgg cgtctcccgc agcgtggaaa agcgcaccaa ggtcaaaagc cgcatcatcg
      421 ccaacgcgct gggggacatc atccgccaga gcgacagcgt catcatcatg ggtcaccggc
      481 tgtccgacct ggacgccatc ggcgcggcca tcggcgtgct gcgcatctgc aagatcttcg
      541 acaaaccggc cgccatcgcg gtggacgaaa agcacacgct ggcgggcagc ctgatcgacc
      601 ggttccgccg cagcggcctg ggggaggatt tcatcacccc ggccgaggcg ctggagggcc
      661 tcacccgtcg cacgctggtg atcattgtgg acacccatgt gccgcagctg ctggagtcgg
      721 gcgagatcta ccgggcggca aacaacgtgg tggtcatcga ccaccaccgc aagatggtgg
      781 ggcacatcga caatgcggtg atcttttatc acgagcccta cgcctcctcc acctgcgagc
      841 tggtggcgga gctgctgcaa tatgtgggcg agcgcgagga ccgacccacc gcccttgaaa
      901 gcgaggccat gctggcgggc attatgctgg atacccgcga ttttgtcatc aacgccgggg
      961 tgcgcacctt tgaggcggcg gcgtatctgc gccggatggg cgcgcagacc caaaacgtga
     1021 agcggctctt cgcgggcagc ctggagagct ataccagcaa ggccaagctg gtcagcgagg
     1081 cggtacttta taagggctgc gccatcgtgg cctccgacga cgtgcccacc gacagcggcg
     1141 tcatcgtgcc gcaggcggcc aacgacctgc tctccatcga gggggtgcag gcgagctttg
     1201 tggcggtgga tatgggcggc ttcatcagtg tgtcggcccg cagcctgggc gaggtgaacg
     1261 tgcaggtcat catggaaaag ctgggcggcg gcgggcacct gaccatggcg ggcgcccaga
     1321 tgaaagacgt cagcctcgag aaaaccaagg tctatatcat ggcggcaatc gacgaatacc
     1381 gcaggaatca ggcggaggag gagtgaatcc cgcccccgca agtttacagg ataaaggagc
     1441 gaagaaaaat gaaagtagtg ctgaagacag atgtgaaagg gaccggcaaa aaggacgaga
     1501 tggtggaggt ctcggacggg tatgcgcgca atttcctgct gccccgcggg ctggccgtgg
     1561 ccgccaacgc cgccgccgtc aatgaggtga ggaccaagga gagcgccaag cggcaccacg
     1621 ccgaggtgga gctggccgac gcgcaggcaa ctgcgaaaaa gatcggcggc atgaccgtca
     1681 cggtgaaagc caaggcgggc cagggcggcc gcctgttcgg cgccgtgacc ggcaaggaca
     1741 tcgcagaggc ggtcaacaag accgcgggcg tctcgatcga caagcgcaag gtcgtgctgg
     1801 acgcggacat caaaaactac ggccagtatg aggtggagat caaagtctac cccagcgtga
     1861 cagccaaggt gacggtcgtg gtagaggaat agtttaagaa ttaaaaaaga gcaaaagact
     1921 tttgctccgc tttgagctgt cacgcggaag cggaggaaaa gacctgggag aacggccccg
     1981 ccgttctcgt cgggccagcg cagagctgag aatcatcggt aaaacccgga ctggcccctg
     2041 tgacagccaa ggtgacagtc gtggtagagg aatagtttaa gaatcaaaaa agagcaaaag
     2101 acttttgctc cgctttgagc tgtcatgcgg aagcggagga aaagacctgg gagaacggcc
     2161 ccgccgttct cgccgggcta gcgtgaagct gaaagtcgcc ggtaaaaccc ggactggccc
     2221 ctgcggcccg tcgggcggaa agacggtcaa caaacggtaa aggaaccccc ttatggacaa
     2281 cctatccctg acatttgaaa atatgggcgt gcagctcccc tacaacatgg aggcggagca
     2341 ggccgtgctg ggcgccgcgt tgctgcaggg ggactgcatc ccgaccctgg tggagaagct
     2401 gcgccccgag cacttttatg tgcgccagaa cggggagatc ttcgccgagc tggtgcgcct
     2461 tttcaccggc ggcgccgcca ttgattttgt cacggttctg ggcgcggtgc tggacgcggg
     2521 cgtctttgcc ggcgaggagg aggccaaggt ctacctcacc ggcctcgccg agacggtgcc
     2581 ctccatctcc aacgtggagc attatgcggg catcgtttac gaaaaatatc tgatccgcca
     2641 gctcatgggg gccgcaaagg acattctgga gcagaccagc gaggagtccg acgcggacat
     2701 tttgctggag agcgcggagc aaaaaatcta tgagattcgc tcggggcgcg accagagcgc
     2761 catgcagccc atctccagca ccatcatcga cacctataac cagctgcaga agatctcggg
     2821 cccggaccgg gacaagtatc tgggcatccc caccggcttt acctatctgg acaagatgct
     2881 caccggcctg ggccgcagcg acctgattat cctggcggcc cgccccggca tgggcaagac
     2941 cagctttgcg ctgaacatcg ccaccaacgt ggcaaaaaaa cagaagatcc cggtggcgct
     3001 gttttcgttg gagatgacca aggaccagct gaccagccgc attttgtcca gcgaggcggc
     3061 catcgacagc cagaagctgc gcaccggcac cctttccggg caggactggg tggacctggc
     3121 ccaggccagc gatatcctgg cccaggcccc catttatatg gacgacacct cggccatcac
     3181 ggtgcccgag atcaaggcca aggtgcggcg gctcaaccag gacccggcca aacccgacgt
     3241 ggggctggtg gtcatcgact atctgcagct gatgaactcg ggcaagcgca ccgattcccg
     3301 cgtgcaggag atcagcgata tcacccgcaa cctgaagatc atggcaaaag agttgaacgt
     3361 gcccatcatc tgcctctcgc agctctcccg caacaccgag cgcagctcca accgcgatca
     3421 ccggcccatg ctatccgacc tgcgggattc gggctcgatc gagcaggatg ccgacagcgt
     3481 gctgtttttg taccgcgagg cgtattatga caccgagggg cagagcgaca cctccaccgc
     3541 cgagtgtatc gtggcaaaaa accgccacgg cgaggtgggc accgtcagcc tgggctggga
     3601 cggcgcccac acccggttca tgaacatcga gtacagccac aatgacggat attgagaagc
     3661 tggaaaaaaa ggcggcgcga tggatagaag agcaggcgct gctgcagagc ggagacacgg
     3721 tgttgtgcgc gctttcgggc gggcccgatt cggtggccct gttccatgcc ctgcgccgcc
     3781 ttgccccggc gttgggcgtg gaactgaggg ccgcgcatct caaccacggc ctgcgggggg
     3841 cggaggcgga ccgggacgaa gcgtttgtgc ggcggctctg cgcccgctgg tcggtgccgc
     3901 tttttgtgga gcgggccggg atggcgctgc gcccgcgccc ggccgggcag agcgaggagt
     3961 ctttcgcccg cgcgctgcgg tatgattttt ttgagcgcgc ggccgccgcc gcacaggcgc
     4021 agggctgcgg gccggtaaag gttgcgaccg cccacaccgc cagcgacaac gccgagacgc
     4081 ttctgttccg cttggcgcgc ggcacctcgc tcaccggcgc cggcggtatc ccgccccggc
     4141 gcgggaggta tatccgcccg ctgctggatt gtacgcgggc ggaggtgttg gcctattgcg
     4201 ccgcgagcgg ctggggttat atgatcgatt ccaccaatct ggcgccctgc tgcagccgca
     4261 atcggctgcg gctgcaggcc gtgcccgcgc tgcgggaggt caacccggat gcggacgcgg
     4321 ccctgtctgc ctttgccgcg gacgcgcggg agacggcggc ctttttggag gggctggccc
     4381 gggagctgct ggcctcggcg g
//
LOCUS       DBMDYA010000161         4398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4763_length_4398_cov_1.605073, whole genome shotgun sequence.
ACCESSION   DBMDYA010000161 DBMDYA010000000
VERSION     DBMDYA010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4763_length_4398_cov_1.605073"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="ACZPFK_05225"
     CDS             complement(<1..215)
                     /locus_tag="ACZPFK_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405744.1"
                     /translation="MNADLDAAARRGEHPLQMWAPLTVRVDGGYEYRLQSRAAKWARA
                     VLYRVIRAIFPPAFRLVYGLRVRGRKN"
     gene            complement(212..1357)
                     /locus_tag="ACZPFK_05230"
     CDS             complement(212..1357)
                     /locus_tag="ACZPFK_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1405745.1"
                     /translation="MNILLVMDQFDAANNGTTISARRFAAGLRQRGHEVRVVACGGPA
                     RDKYEVPAIHFTPIVDGIIRSQGMRLAMPKKKVLREALAWADVAHFMMPFALSRGGLR
                     LAEQMGVPHTAAFHVQPENITYTLGLGRSARLNDAIYRELRDDFYDRFTHIHCPSDFI
                     AGELKKHGYKAAVHVISNGISPRFCYRKRGKTPALQGKEVVMMIGRLSNEKRQDVLID
                     AVSRCRHRDSIQLVLAGQGPKKAALERQGRRLPNPPIFGFFPEERLRELLSMTDLYVH
                     ASDAEIEAISCIEAFASGLVPVIADSPKSATPQFALDRRSLFAAGDSVDLASRIDYWL
                     DHPAERRGMELRYAEAGRRYHLDVCVAQAEEMFRAAIAEAGTAVEKA"
     gene            complement(1708..2694)
                     /locus_tag="ACZPFK_05235"
     CDS             complement(1708..2694)
                     /locus_tag="ACZPFK_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405746.1"
                     /translation="MQIKDIARMAGVAPSTVSRVINNSGYVSAEVRGRVKEVIRRTGY
                     VPNSIAKSLKAKRSHTIGVIVPRIASEAMARQIQGIAGECEQRRYEMLLANAGMKTEN
                     ESACLEMLARRQIDGVVIMSSGVTADLRLRIDALEAPVVVTGQQAEGLCCVVHDEAGI
                     MREITTALLLKGRRDPAFINVELEDDAIRHKRFSGFSEALRQQRLALRPGRVAYGGFT
                     IEEGFAAMKQLWEQEGPHPDAVLAVTDRAAVGAMQYLKSRGVRLPQQCAVTGIGNNQL
                     AAVVEPALTTVEYFYEEVGAKAARLLFEAIDAGARPQGVHFVDYRLIERASA"
     gene            2965..4059
                     /locus_tag="ACZPFK_05240"
     CDS             2965..4059
                     /locus_tag="ACZPFK_05240"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HIR1405747.1"
                     /translation="MSAVDELQQARAEINEVDAGMAALFERRMAAVRRVLEYKRAHGL
                     PVLDAGREKEVLARGAARVEDPALREYYARVLERLMEVSREFQAKQLGGFTVGYPGVE
                     GAFSHIAASRIFPGKKQQAFVTFEEVFRAVQGGAVQAGVVPFENSYTGEVGEVLDLLW
                     RYDCSIVEMYDLDIRQNLLALPGARLEEIRQVYSHHQAISQCQTWLAGRDFEVVPYPN
                     TALAAKYVSECGDCAKAAIASADTAALYGLSILARDINTSAQNVTRFIVLSRTPQHRG
                     NRFNLVFTVRHDAGALARAMQIIGSRGFNLESIRSRSMRELPWQYFFYAEVVGDPASP
                     EAKEMLDELAAACSQLKLLGAWERKEAGGR"
BASE COUNT          771 a         1405 c         1421 g          801 t
ORIGIN      
        1 aaatttttac gaccgcgcac ccgcaggccg taaaccaggc ggaacgccgg cgggaagatc
       61 gcgcggatca cccggtacag cacggcgcgc gcccactttg ccgcccggct ctgcaaccgg
      121 tactcatatc cgccgtccac ccgcaccgtg aggggcgccc acatttgcag cggatgttcc
      181 cccctgcggg cagcggcatc cagatcggcg ttcacgcttt ttccaccgcc gtgcccgcct
      241 cggcgatggc cgcgcggaac atctcctccg cctgggcgac acagacatcc agatgatacc
      301 gccgccccgc ctcagcatag cgcagctcca tcccgcgccg ctctgccgga tgatccagcc
      361 aataatcgat gcggctggcc aggtccacgc tgtccccggc cgcaaagaga ctgcgccggt
      421 ccagggcgaa ctgcggcgtg gcggacttgg gggaatctgc gatcaccggc accaggccgc
      481 tggcaaaggc ttcgatgcag gagatggcct cgatctcagc gtccgaggcg tggacgtaca
      541 ggtcggtcat ggagagcagc tcgcgcaggc gctcctcggg gaaaaagccg aagatcggcg
      601 ggttgggcag ccgtctgccc tggcgctcca gcgccgcttt tttcggcccc tgccccgcca
      661 gcaccagctg gatgctgtcc cggtgccggc agcgggagac ggcgtcgatg agcacatcct
      721 gccgcttttc gttggacagg cggccgatca tcatcacgac ctccttgccc tgcagggcag
      781 gggtcttgcc gcgcttgcgg tagcaaaacc gggggctgat gccgttggag atcacgtgga
      841 ctgccgcctt gtagccgtgc tttttcagct cgccggcgat aaaatcactg gggcagtgga
      901 tgtgggtgaa ccggtcgtaa aaatcgtccc gcagctcgcg gtagatggcg tcgttgagcc
      961 gcgccgagcg ccccaggccc agcgtgtagg tgatattctc gggctgcaca tgaaaggccg
     1021 cggtgtgggg cacccccatc tgctcggcca gcctgagccc cccgcgggag agggcgaacg
     1081 gcatcatgaa gtgcgccacg tccgcccagg ccagggcctc gcgcagcact ttctttttcg
     1141 gcatcgccag ccgcatgccc tggctgcgga tgatgccgtc cacaatgggc gtgaaatgga
     1201 tggccggcac ctcatacttg tcccgggccg ggccgccgca ggccaccacg cgcacctcgt
     1261 ggccgcgctg gcgcagcccg gcggcaaagc gccgggcgct gatggtggtg ccgttgttgg
     1321 cggcgtcaaa ctggtccata accaacagga tattcatcaa atcccccaaa tctgtaaagc
     1381 ggcgacccgg tgggcgggcg gccgctccta gcctccgcgc agatcgggcc caagcccggc
     1441 gcggcggtat catctttatc acggcgcaat aaaaaacgac ttatttttat ggtccctgcc
     1501 agaggactgg ccgaatggac aaaaaatagc ccgacagcta ttttttatcg ccctgcaata
     1561 aagaacaact tatcttaatc ttgatggccc agtttctcgc cgctgacgcg gcaaccgaaa
     1621 cggatggccg aaaactagcc tggcggctat tttttcagta tagcatggga aaggggcgca
     1681 gtatccagtc aatcgccccg atctgtctca ggccgaggcg cgctcgatca gccggtaatc
     1741 gacaaagtgg acgccctgcg ggcgggcccc ggcgtcgatg gcctcaaaca gcaggcgggc
     1801 ggccttggcg cccacctcct cataaaaata ctccacggtg gtgagcgcgg gctccaccac
     1861 cgcggccagc tggttgttgc cgatgccggt caccgcacac tgctgcggca gccgcacccc
     1921 ccgcgattta aggtactgca tggcccccac cgccgcccgg tcggtgactg ccagcacggc
     1981 gtccggatgg gggccctcct gctcccagag ctgcttcatg gcggcaaagc cctcttcaat
     2041 ggtgaagccg ccgtaggcga cgcggccggg gcgcagggcg agacgctgct gccgcagcgc
     2101 ctcgctgaag ccggaaaacc gcttgtggcg gatggcgtcg tcctccagct ccacgttgat
     2161 aaaagccggg tcgcggcgcc ccttgagcag cagcgccgtg gtgatctcgc gcatgatgcc
     2221 cgcctcgtcg tgcaccacgc agcacagccc ctccgcctgc tgcccggtaa ccaccacagg
     2281 ggcctccagc gcgtcgatcc gcagccgcag gtccgccgtg acgcccgagg acataatgac
     2341 cacgccgtcg atctgccgcc gcgccagcat ctccaggcag gcggattcgt tctctgtctt
     2401 cattcccgcg ttggcgagca gcatctcata gcggcgctgc tcgcactcgc ccgcaatgcc
     2461 ctggatctgg cgggccatcg cctcggaggc gatgcgcggc acgatgaccc cgatggtgtg
     2521 gctgcgcttt gccttgaggc ttttggcaat gctgttgggc acatagccgg tgcgccggat
     2581 cacctccttg acccgcccgc gcacctctgc gctgacatag ccggagttat tgatgacgcg
     2641 ggaaaccgtc gagggagcca cccccgccat gcgcgcgatg tccttgatct gcaaagtgcc
     2701 gcacttccat tcgtcaaaaa tttcacatat tgtctcactg ggtagtatag cagagctgga
     2761 atcgtttttc tagtgtcatt ttgccgggaa atgggcggtt tgtttttacc tcgtttacaa
     2821 tatttgacaa aatagtaaga atttgtcgcc gtcaaggcgc gctggaaagc aaaaacagac
     2881 atttcccggg acagtgcggg cccagggctt gcgccgggcg gcgggtggtt gctataatag
     2941 cctcaaacgc aacaggcaag ggggatgagc gcggtggacg agctgcagca ggcgcgcgcc
     3001 gagatcaatg aggtggacgc ggggatggcg gcgctctttg agcggcgcat ggcggcggtg
     3061 cggcgggtgc tggagtacaa gcgcgcccac ggcctgccgg tgctggacgc ggggcgggaa
     3121 aaggaggtgc tggcgcgcgg ggccgcccgg gtggaggacc cagccctgcg ggagtattac
     3181 gcccgggtgt tggagcgcct gatggaggtc tcgcgggaat ttcaggccaa gcagctgggc
     3241 gggttcacgg tgggctaccc cggtgtggag ggggcgttct cccacattgc ggcttcccgc
     3301 atctttcccg gcaaaaagca gcaggccttt gtgacctttg aggaggtctt ccgcgccgtg
     3361 cagggcggcg cggtgcaggc gggcgtggtg ccctttgaaa actcttatac cggcgaggtg
     3421 ggcgaggtgc tggacctgct ctggcggtac gactgctcca ttgtggagat gtacgacctg
     3481 gacatccgtc agaacctgct ggccctgccg ggggcgcggc tggaggagat ccggcaggtc
     3541 tactcccatc accaggccat cagccagtgc cagacctggc tggccggccg ggactttgag
     3601 gtggtgccct accccaacac cgccctggcc gcaaaatacg tcagcgagtg tggggactgc
     3661 gccaaggcgg ccatcgccag cgcggacacc gcggcgctgt acgggctgtc gatcctggcg
     3721 cgggacatca ataccagcgc gcagaacgtg acccgtttta tcgtgctctc ccgcacccca
     3781 cagcacagag gcaaccgctt taacctggtg tttacggtgc ggcacgacgc gggggcgctg
     3841 gcgcgggcca tgcagatcat cggcagccgc ggcttcaacc tggagagcat tcgctcgcgc
     3901 tcgatgcgcg agctgccctg gcaatatttt ttttatgccg aggtggtggg cgaccccgcc
     3961 tcccccgagg caaaggagat gctggacgag cttgccgccg cctgcagcca gctgaagctg
     4021 ctcggtgcct gggagagaaa agaggcgggc ggccgttgac gttgccggcg gcgcaggagg
     4081 cgcaggaggc gtgggaggcg cgccggctgt cgcccatggc gcgaggcgcg atttctgcgg
     4141 ccgcccggct gtcttttgcc gccaaatctt ttcgttgaca agcggcttat tttcctctat
     4201 actgatacca atccgcgatt cagggagagt tatccatgag acagttcaac accaacgcag
     4261 cgcgctttta ctttagctac ggctttgctt attattttgg caaggcccgt tctgtgctgc
     4321 gcaaaaccgg tttgaacatg cggtgagctt tgacaccttt gtttcaatgc acggcggcct
     4381 gcacagcagg ccgccgtt
//
LOCUS       DBMDYA010000162         4395 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4767_length_4395_cov_1.467163, whole genome shotgun sequence.
ACCESSION   DBMDYA010000162 DBMDYA010000000
VERSION     DBMDYA010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_4395_cov_1.467163"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="ACZPFK_05245"
     CDS             <1..789
                     /locus_tag="ACZPFK_05245"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HIR1405748.1"
                     /translation="VPDPVLKKIQRSISANVLAYMPVSPWATAYRPGGGVRRNALPHC
                     GQEQLLKLDIRRFFDSVRYTQVKETVFPAAIYAEKLRVLLSILCYYKEGLPQGAPTSP
                     AIANILLRRFDETVGGWCGAHGVAYTRYCDDLAFSGSFDPAALAALVREALRREGFSL
                     NGQKTRLAGPGRRQLVTGAVVNLRPRAPAEDRRALRQELYYCQKFGVRGHLERTRDPR
                     PPQAYLAALLGRLDFALSLSPEPQLAQGRRWVLDRLRQERAFHP"
     gene            complement(1270..2088)
                     /locus_tag="ACZPFK_05250"
     CDS             complement(1270..2088)
                     /locus_tag="ACZPFK_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1405749.1"
                     /translation="MLKIGIFSKLSRISIRMLRYYDDAGLLSPKRVDPFTGYRYYGED
                     QLPDAERINALRQMGFGIAAIARTQGRADDREQLLLLLKAKEAELCREAGEAAERLRL
                     LRTAMSHLRKDETMTQYNVTLKELPERAVASVRKKIPSYDCEQQLWDILMRETAPLKL
                     EPGEPCLTLAIFHDGEYKESDVEVEVQKTVKGSYPDTEHVRFKTVPAVRIASATYQGG
                     YEQITEVNKAVAAWVQENGYEFDGLSFCIYHVSPYDTSDPGQYVTEVCYPVRRG"
     gene            complement(2517..3068)
                     /locus_tag="ACZPFK_05255"
     CDS             complement(2517..3068)
                     /locus_tag="ACZPFK_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405750.1"
                     /translation="MLTIMLVIFVAAVPGTAALIFFKRAKREEDELLRVRESRAVAVL
                     GGLLWAFALGMYLYCLLAPGRLYTTESGAVNVAVFLGMGLAFGAAALLTYFVKCTVAT
                     ERGVLAVGLLGGRELLAWRNIIKLQEANGRLMLLDAKGRQCSVGGNQEQLRQFVQLAK
                     ERLRPGVGAAVLKSMEGKLAKKK"
     gene            3349..3759
                     /locus_tag="ACZPFK_05260"
     CDS             3349..3759
                     /locus_tag="ACZPFK_05260"
                     /inference="COORDINATES: protein motif:HMM:NF023580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR1405751.1"
                     /translation="MGELFVVAAVLAGLGLLCFFAGALYWRAALRRDRRCTARVQGLV
                     TELAATGNGVRVTLCPVLRYAVDGRELTRRSGFASNRCPFTTGQRLEVRCDPQDPRVF
                     VLEGELAHRRGLALRLALVGGGFLLLALVALASG"
BASE COUNT          681 a         1556 c         1372 g          786 t
ORIGIN      
        1 gttcccgacc ctgtcctcaa aaagattcag cgcagcatca gcgcgaatgt tttggcctat
       61 atgcccgtct ccccctgggc caccgcctac cgccccggcg gcggcgtgcg gcgcaacgcc
      121 ctgccccact gcgggcagga gcagctgctg aagctggaca tccgccgctt ttttgacagc
      181 gtgcgctaca cccaggtcaa ggagacggtc ttccccgccg cgatctacgc cgagaagctg
      241 cgggtgctgc tgtccatcct ctgctattac aaggaggggc tgccccaggg cgcgcccacc
      301 tcgcccgcca tcgccaacat cctgctgcgg cggttcgacg agacggtggg cggctggtgc
      361 ggcgcgcacg gcgtcgccta cacccgctac tgcgacgatc tggccttttc gggcagcttt
      421 gacccggcgg cgctcgccgc cctggtgcgg gaggccctgc gccgcgaggg cttttcgctg
      481 aacgggcaga agacccggct ggcggggccg ggccgcaggc agctggtcac cggcgcggtg
      541 gtgaacctgc gcccccgcgc cccggccgag gatcgccgcg ccctgcggca ggagctgtac
      601 tactgtcaga aattcggggt tcggggccac ctggagcgca cccgcgaccc ccgcccgccg
      661 caggcgtatc tggcggcgct gctggggcgg ctggattttg cgctcagcct ctcgccggag
      721 ccgcagctcg cccagggccg ccgctgggtg ctggaccgcc tgcggcagga gagggccttt
      781 catccttgaa gccgggggcc ggcaggcgtt cacgcagttc cgacagggaa ccgccgccgg
      841 gttctgacgg gaaaggccgc ctgctgaggc ggcttcgcct gcaaacccca tcgacatgcg
      901 gcgccccatt cctttcgggg cgaacctctt tccgcgggcg gcccacgccg tgtggttttc
      961 cccaaccgcc ctgcctgcgc aaaggcggac ggcagccggg gggtgccgga tcagaacacg
     1021 ggctgcggtg cgaaccgacc ggcccctttt ttatgcccga acccggcctt gagcgcacga
     1081 gctttttgcg agaaaggccg gtatcagtgc ggctgttttt gcccgtcccc ggacggcggc
     1141 gaaagagaac tgcaacgccg gggctgccgg gccggccgca gatttttccg cggctccagg
     1201 cggcgacaac agagagggcg ggcatcccgg gatgcccgcc ctctcgtgtt tttccgtccg
     1261 cgcgccgggt tatccccgcc gcaccgggta gcagacctcg gtgacatact gccccgggtc
     1321 gctggtgtcg taggggctga catggtagat gcaaaaagac agcccgtcga attcatagcc
     1381 gttctcctgt acccaggccg ccaccgcctt gttcacctcg gtgatctgct catagccgcc
     1441 ctggtaggtg gccgaggcga tgcgcacggc gggcacggtc ttgaacctca catgctccgt
     1501 gtcgggatag ctgcccttca cagttttctg cacctccacc tccacatcgc tctctttata
     1561 ctccccgtcg tgaaaaatcg ccagcgtcag gcagggctcc ccaggctcca gcttcaaggg
     1621 cgccgtctcc cgcatcagga tatcccacag ctgctgctcg cagtcataag agggaatctt
     1681 ttttcgcacg ctggccaccg cccgctcggg cagttccttc aaagtcacat tgtactgggt
     1741 catggtctca tcctttctca gatggctcat cgccgtccgc agcagccgca gccgctcggc
     1801 ggcctccccg gcctcccggc acagctcggc ctcctttgct ttcaaaagca gcagcagctg
     1861 ttcccggtcg tccgcccgcc cctgtgtccg ggcgatggcg gcgataccga accccatctg
     1921 ccgcaaggcg ttgatccgct ctgcatcggg cagctgatcc tcgccgtaat agcggtaccc
     1981 ggtaaacggg tcgacccgct tgggcgacag caggcccgcg tcgtcgtaat agcgcagcat
     2041 acggatgctg atgcgggaca gctttgaaaa aataccgatc ttcagcatgg tggtctcctc
     2101 acatatatgt ggtcgctcct gagctcaaca cccagtataa aggatgtcac ggtgtcagag
     2161 tcaagggggg aaaacacaaa aattgcaggc gccctcaaaa gcgcctgcat cgagtgggtc
     2221 gaacaagaaa acgcgccctt gggcgctgtg cggcaaaaaa gcattggttt gggagcggcg
     2281 gttttgacgc tccccatctt taagccctgt gtctctttca aagccgccgc gcgcccccgg
     2341 gagggaaagc cgcgatgcgg cgctgggaaa gttcacgcgg ccggcctgcc ttgcgcgcgc
     2401 gggtcgcggg gtgaaaccgc agcccattct gcggccgccg gaggaaagga aaggtactcc
     2461 gcgcacaagc catcactctc agggaacaat tttttgcaca aacgcgcccc tgcccgctat
     2521 ttcttttttg ccagctttcc ctccatgctt ttcagcacgg ccgcccccac gcccgggcgc
     2581 aggcgctctt tcgccagctg cacaaactgc cgcagctgct cctggttgcc gcccaccgag
     2641 cactggcgcc cctttgcatc cagcagcatc aggcggccgt tcgcctcctg cagcttgatg
     2701 atgttccgcc aggcaagcag ctcgcggccg cccagcaggc ccaccgccag caccccgcgc
     2761 tcggtggcga cggtgcattt gacaaaatag gtgagcagcg ccgcagcgcc gaacgccagc
     2821 cccatgccga gaaagacggc cacgttcacc gccccgctct cagtggtgta aagcctgccc
     2881 ggcgccagca ggcagtagag atacatcccc agcgcaaatg cccagagcag cccgcccagc
     2941 acggcgacgg cgcggctctc ccgcacccgc agcagctcat cctcctcccg cttcgccctt
     3001 ttgaaaaaga tcagggcggc cgtgcccggc accgccgcca caaagatcac cagcataatg
     3061 gtcagcattg atcgaacgct ccctctgtcg cgccccggcc gcaggccgca gggcgcgttt
     3121 tggagtattg tagcatatcc cgccgaaaaa gcaaaatgcc cgccccgccg ttttgccgcg
     3181 cgcgatccct ccctgcggcg gccctccccc gccggcaagg caaaaaaacg ctctcaaaaa
     3241 agcgccgttt ccctcagccc gctcctccgg gcggcccggc cgtggttttg cagtgggcgc
     3301 ggccccgtgg tatactgggt gcatcacgct tttgggaggc tgtccgccat gggagaattg
     3361 tttgtcgtcg ccgccgtcct ggccggcctg ggcctgctct gcttttttgc cggcgcgctc
     3421 tactggcggg ccgcgctgcg ccgggaccgg cgctgcaccg cccgggtgca ggggctggtg
     3481 accgagctgg ccgccaccgg caacggcgtc cgggtcaccc tctgcccggt gctgcggtac
     3541 gcggtggacg ggcgcgagct gacccgccgt tccggcttcg cctccaaccg ctgcccgttc
     3601 accaccgggc agcggttgga ggtgcgctgc gacccgcagg acccgcgggt gttcgtgctg
     3661 gagggcgagc tggcccatcg ccgcggcctc gccctgcgcc ttgccctggt ggggggcggc
     3721 tttctgctgc tggcgctggt cgcgctggcc tcgggctgag cgcgccgctt cccggcccaa
     3781 tccaagttca atccaaactg ttctttgcgc atccggccag acgggacgca aagtgtcgcc
     3841 tgcggacggg tggaagcgcc gtttttgtcg tcttgccgga gaactcccgg cctctccggc
     3901 cgcggggcgc aaggtgccgc ttgcgaatta gaggagaaca ccgtttttgt tgtcccgacc
     3961 acaagccccc ggtctttccg gccgcggggc cgcactgcct gcaccgtccg ctgcgcgctg
     4021 gagcctaccc gtccggcttt ttccgcgccc ctcgcggctc acggagcaga cgccgcccgc
     4081 gatcggccca tggaggaaaa aacaaagaaa aacgattgcg gggggagggg cactggggag
     4141 gccgcgccgc ttttaacctc tacccatcct ggacagcggg gccgaaccgg cgctttttcc
     4201 ccggcttgat ttctctttcg gcagggcgct ttccgcgcgc ccgcccccgc cccggcacag
     4261 cacatcaaaa tcggcggcag gcctttcgcc tgccgccgat tttgttttgt cccttgctgt
     4321 gcgggggcac ggccgccccc gggcgggagg atcaatccgt ctttttggaa tccgtctgct
     4381 ggacggcgcg gtgat
//
LOCUS       DBMDYA010000163         4393 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4771_length_4393_cov_1.454567, whole genome shotgun sequence.
ACCESSION   DBMDYA010000163 DBMDYA010000000
VERSION     DBMDYA010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_4393_cov_1.454567"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="radA"
                     /locus_tag="ACZPFK_05265"
     CDS             complement(<1..1149)
                     /gene="radA"
                     /locus_tag="ACZPFK_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1405752.1"
                     /translation="MAKEKLKTIYVCTSCGEQSPRWMGRCPSCGEWNTMVEDVVRQPA
                     GGVKAVKAPVLAGEVRSARLYDIDTNDERSRIVTGISELDRVLGGGIVIGSVILLGGE
                     PGAGKSTLLLQICSSIPAGLQVLYVTGEESVRQIKLRANRLGVSGENVTVAAETEIES
                     ITELIRSLHPDLVVVDSIQTMHSGAVSSSAGSVSQVKECSGQLLNVAKTLEIPVFIVG
                     HVNKDGAIAGPKVMEHIVDTVLYFEGDRMLPYRILRAVKNRYGSTNEIGMFDMAGDGL
                     REVVNPSKLLLEGRPLGVSGNCVACTMEGTRPILSEIQALVTKSSFPSPRRTTSGFDF
                     NRANLLLAVIEKRGGYFLGTLDVYLNIVGGLRLDETACDLAVILAVVSS"
     gene            complement(1154..1312)
                     /locus_tag="ACZPFK_05270"
     CDS             complement(1154..1312)
                     /locus_tag="ACZPFK_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405753.1"
                     /translation="MKLREKFLIALIVLALGLLVYRVADGLMGRALLNAARPAAAYSV
                     DARLFDGG"
     gene            complement(1356..>1626)
                     /locus_tag="ACZPFK_05275"
     CDS             complement(1356..>1626)
                     /locus_tag="ACZPFK_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1405754.1"
                     /translation="ELLCDGKTPYEIADAVLEGFSPNVLDESEVQYHCDCSRDRVERA
                     YMSLGQEELRKIAAEQETVELKCQFCNKAYHFRAADYVKPEEAKG"
     assembly_gap    1627..1636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1637..2248)
                     /locus_tag="ACZPFK_05280"
     CDS             complement(<1637..2248)
                     /locus_tag="ACZPFK_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1405755.1"
                     /translation="MSKLIRAISENGGAVVSILDSTDIVAEVERLHHPSAVVSAALGR
                     LVTGAVLMAATLKNEEDALTLRVKGDGPAGLLLAVADGRGNVKGYVENPIVELPPRAD
                     GKLDVGGAVGREGTLTVIKDIGLKEPYVGQVPLVSGEIAEDLTSYYATSEQIPTVCAL
                     GVLVERDLTIKKAGGYLLQLLPGATEQEISMLEQNVSRMKSMTE"
     gene            complement(2236..2991)
                     /locus_tag="ACZPFK_05285"
     CDS             complement(2236..2991)
                     /locus_tag="ACZPFK_05285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1405756.1"
                     /translation="MAGYGDFAYFYDEFNGEADYDALYSFIQERFSSHGLPGPIVADL
                     GCGTGELTLRLARDGYDLIAVDGSPDMLAMLSEKLAEEGRQGVLLLEQDLTCLDLYGT
                     IHGAVCTFDTLNHLGPLEAMGRAIEKVGLFMEKGGLFIFDVNTPYKHRCILGNETFTL
                     EDEDAACIWTNELQEEQSRTRISIDIAYAGDPVHCRECFYEYYCAPQELEAVCVKHGF
                     RVLEVVDGERFCAPGPESQRLLFVAEKEIQCQS"
     gene            complement(3036..3623)
                     /gene="yihA"
                     /locus_tag="ACZPFK_05290"
     CDS             complement(3036..3623)
                     /gene="yihA"
                     /locus_tag="ACZPFK_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1405757.1"
                     /translation="MRYSQAAFKASYGLSSQLPDHDRPEIVFSGRSNVGKSSLINKLC
                     NRKSLARVSATPGKTATINYYDLGDAYLVDLPGYGYAKVSASERARWDDLINSYFAVS
                     ERCTLLIQLLDSRHAPSADDRMMLEYLRHHGIPFVAALTKADKLKKAEAERATGEFAA
                     LCAEYRCKGVVLTSAQDGRGVEELQRVIEAAAADF"
     gene            complement(3634..>4393)
                     /locus_tag="ACZPFK_05295"
     CDS             complement(3634..>4393)
                     /locus_tag="ACZPFK_05295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR1405758.1"
                     /translation="AITALIDGYTQEAGVRGLERVIGEVLRKCAKSIASGETEGELSV
                     TGKSIEPLIGPVRVKPNFSNKTNAVGIANGLAWTSVGGELLPIEVQIIENGSGGLELT
                     GSLGEVMKESAKLAITYAKVHASEYGFESTVFKTIDIHIHAPEGAIPKDGPSAGVTLA
                     TALISALSGRPVRSDVAMTGEITLHGLVLPIGGLKEKTMAAYREKMHTVLVPADNMSD
                     LYEVDEVVKEALRFEPVTDLKAVLKTALLPKKEE"
BASE COUNT          813 a         1467 c         1265 g          838 t
ORIGIN      
        1 gctcgatacc acagccagga tgaccgccag atcacaggcg gtctcgtcca gccgcaggcc
       61 gccgacgata ttcagataaa catccagcgt gccgagaaaa taaccgcccc gcttttcgat
      121 gacggccaac agcagattgg cgcggttgaa atcaaagccc gaggtcgtgc gccgcgggga
      181 gggaaaactg ctcttggtga ccagggcctg gatctcggaa aggatcggcc gggtgccctc
      241 catggtgcag gccacacagt tccccgagac gcccaggggc cgcccctcca gcagcagctt
      301 tgagggattg accacctcgc gcaggccgtc ccccgccata tcgaacatgc cgatctcatt
      361 ggtggagcca taccggtttt tgaccgcccg cagaatccgg tagggcagca tccggtcgcc
      421 ctcaaagtac aacaccgtgt ccacgatatg ctccatgacc ttggggcccg cgatggcgcc
      481 gtccttgttg acatgaccca cgataaacac cgggatctcc agcgttttgg ccacattcag
      541 cagttggccc gagcactctt tcacctgcga aacgctgccc gccgacgagg agaccgcgcc
      601 cgaatgcatg gtctggatgg aatccaccac caccagatcc ggatgcaggg agcggatgag
      661 ctctgtgatg gattcgatct cggtctcagc cgccaccgtc acgttttcac cgctgacgcc
      721 cagccggttg gcgcgcagct tgatctggcg caccgactcc tcgccggtga cgtacagcac
      781 ctgcagcccg gcggggatcg aggagcagat ctgcagcagc agggtggatt tgcccgcccc
      841 cggctcaccc ccaagcagaa tgaccgagcc gatgacaatg ccgcccccga gcacccgatc
      901 cagctcagag atgccggtga cgatgcgcga gcgctcgtcg ttggtgtcga tgtcgtacaa
      961 cctggcggag cgcacctccc cggccagcac cggggccttg acggccttga cgccgcccgc
     1021 tggctggcgc acaacatcct ccaccatggt gttccactcg ccgcagctgg gacagcggcc
     1081 catccagcgc gggctctgct ccccacagga ggtgcagacg tagatcgttt tgagtttttc
     1141 ttttgccaag ggattatcct ccgtcaaaca agcgcgcgtc caccgaataa gcggccgcag
     1201 ggcgggccgc gttcagcagc gccctgccca tcaggccgtc cgccacccgg tacacgagca
     1261 gccccagagc taaaacgatc agcgcgatca aaaatttttc tctcagcttc acggctttca
     1321 cctcacatct caatgatatg aggcgagcgc cctctttacc cctttgcctc ttccggcttg
     1381 acataatccg cggcgcggaa gtgataggcc ttgttgcaga actggcattt cagctccacc
     1441 gtttcctgct cggcggcgat cttgcgcagc tcctcctggc ccaggctcat ataggcgcgc
     1501 tccactctgt cccggctgca gtcgcagtgg tattgcacct cgctctcgtc cagcacattg
     1561 ggcgaaaagc cctccagcac cgcatccgcg atctcatagg gcgtttttcc gtcgcagagc
     1621 agctcgnnnn nnnnnnctcg gtcatgctct tcatccgact cacgttctgc tccagcatgc
     1681 tgatctcctg ctcggtcgcc cccggcagca gctgcagcag gtagcctccc gcctttttga
     1741 tggtcaggtc gcgctccacc agcacgccca gcgcgcagac ggtggggatc tgttcgctgg
     1801 tggcgtaata ggaggtcagg tcctcggcga tctcgccgga gaccagcggg acctggccca
     1861 catacggctc tttgaggccg atatccttga tcacggtgag ggtgccctcc ctgccgacgg
     1921 cgccgcccac gtccagcttg ccgtccgccc ggggcggaag ctccacgatg gggttttcca
     1981 catagccctt gacgttgcct cggccgtcgg caacggcgag cagcaggccc gcagggccgt
     2041 ctcccttgac ccgcagcgtg agcgcgtcct cctcattttt cagggtggcg gccatcagaa
     2101 cagcgccggt caccaggcgc cccagcgccg cagagaccac cgcgctgggg tgatgcagcc
     2161 gttccacctc cgcgacgatg tcggtggaat ccaaaatcga gaccaccgcg cccccatttt
     2221 cagaaatcgc ccttatcagc tttgacattg tatctccttt tccgccacaa aaagcaggcg
     2281 ctggctctcc ggcccgggcg cgcaaaagcg ctcgccgtcc acgacctcca gcacccggaa
     2341 gccgtgcttt acacaaacgg cttccagctc ttgcggcgca caataatatt catagaaaca
     2401 ctcgcggcag tggaccgggt cccccgcata cgcgatatcg atcgagatac gggtgcggct
     2461 ctgctcctcc tgcagctcgt tggtccagat gcaggccgcg tcctcgtcct ccaatgtaaa
     2521 agtctcgttg cccaaaatgc agcggtgctt gtagggggtg ttgacatcaa agataaaaag
     2581 cccgcccttt tccataaaaa ggcccacttt ttcaatggcg cgccccattg cctccagcgg
     2641 ccccagatga ttgagggtat cgaacgtgca gacggccccg tggatcgtgc cgtaaaggtc
     2701 caggcaggtc aagtcctgct ccagcagcaa cacgccctgc cggccctctt ccgccagttt
     2761 ttcgctgagc atcgccagca tatccggcga gccgtccacc gcgatcaggt catagccgtc
     2821 ccgggcgaga cgcagggtca gctcccctgt gccgcagccc aggtcggcca caatggggcc
     2881 gggcagccca tggctggaaa agcgctcttg aataaaggaa tagagggcgt cgtagtcggc
     2941 ttccccgttg aactcatcat aaaaataggc aaagtcgcca tatcccgcca aacagagcac
     3001 cgcctttcct tttttcgata aaaacggggg ctgctttaaa aatcagcggc ggccgcttcg
     3061 atgacccgct gcaattcttc gacgccgcgg ccatcctgcg cgctggtaag cacaacgcct
     3121 ttacatctat attctgcgca aagagcggcg aactcgcctg tcgcgcgctc agcctctgcc
     3181 tttttcagct tgtccgcctt ggtgagcgcg gccacaaagg ggatgccgtg gtggcgcaga
     3241 tactccagca tcatcctgtc gtccgcggag ggcgcgtggc ggctgtccag cagctggatc
     3301 agcagagtgc agcgctccga cacggcaaaa tagctgttga tgaggtcgtc ccagcgcgcc
     3361 cgctcggagg cggagacctt ggcgtagcca taaccgggca gatccaccag ataggcgtcc
     3421 cccaggtcgt aataattgat ggtggcggtt ttgcccggtg tcgcgctgac ccgggccagg
     3481 ctcttgcggt tgcagagctt gttgatcagc gaggacttgc ccacgttgga gcggccggaa
     3541 aagacgatct caggccggtc gtggtcgggc agctgggacg aaagcccata agaggccttg
     3601 aaagcggcct ggctgtatct cataaatgaa acctcactct tcctttttcg gcagcagggc
     3661 ggtcttcagc accgctttga ggtcggtcac cggctcaaag cgcagcgcct ccttgaccac
     3721 ctcgtccacc tcatagaggt cggacatatt gtctgccggc accagcacgg tgtgcatttt
     3781 ttcgcggtag gcggccatgg tcttttcttt caggccgccg atgggcagca ccagcccgtg
     3841 cagcgtgatc tcgccggtca tggccacgtc gctgcgcacc gggcggcccg agagcgcgga
     3901 gataagcgcc gtcgccaggg tgacgcccgc cgacgggccg tccttgggaa tggccccctc
     3961 cggcgcatgg atgtggatgt cgatggtctt gaagaccgtg ctctcaaaac cgtattccga
     4021 ggcgtgcacc ttggcgtaag tgatggccag cttggccgac tctttcatca cttcgcccaa
     4081 agagccggtc agctccaggc cgccgcttcc gttctcgatg atctgcacct caatgggcag
     4141 cagctcgccc ccgaccgagg tccaggcgag gccgttggca atgcccaccg cattggtctt
     4201 attggaaaag ttcggcttta cgcgcaccgg gccgatcagc ggctcgatgc tcttgccggt
     4261 gacggacagc tccccctccg tctccccgga ggcgatggat ttggcgcact tgcgcagcac
     4321 ctcgccgatc acccgctcca gcccgcgcac gccggcctcc tgggtgtaac cgtcgatgag
     4381 ggcggtaatg gcg
//
LOCUS       DBMDYA010000164         4378 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4792_length_4378_cov_1.580781, whole genome shotgun sequence.
ACCESSION   DBMDYA010000164 DBMDYA010000000
VERSION     DBMDYA010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_4378_cov_1.580781"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="ACZPFK_05300"
     CDS             complement(<1..385)
                     /locus_tag="ACZPFK_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024099.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HIR1405759.1"
                     /translation="MNSGSEAADQMVREGIQITEAAVKLSALGAKNLAAIALALARDN
                     PKVRGRTRLSRLLRDGKELKVFPIQAGDLAEFKRQAKHYGVLFSAIKDKSSEGELIDV
                     LARAEDVSKLNRIFERMGYPAPKRRV"
     gene            complement(382..1815)
                     /locus_tag="ACZPFK_05305"
     CDS             complement(382..1815)
                     /locus_tag="ACZPFK_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIR1405760.1"
                     /translation="MAYTKIKAVKNHLKRCLDYAANPAKTKTGQDLQDALTYAHNSDK
                     TEQQLFVTGFNCDPAHACEVMLRTETIWGKGGENHVLGYHVIQSFAPGEVTPKQAHEI
                     GCEFARRAFAERYEATVSTHLNTGTLHNHIVFNSVSFVDGKMFRNDFKGYFKGIRAVS
                     DQLCRERGLSIIDPKQKGKAYIERQAEKEDKPTARSMIRADVNRALARAVSWETFVAG
                     LRKMGYSVRYGPNIKYATLRHKTGSRNIRLKSLGEGYDEASIRNLLERRARGEEVKLP
                     RAAEDAARTRSIRDKPIPEVPARRVRRVRLRGALPVVPRRKYTGFMALYYRYVYLLGK
                     TRQRRTSRRCYFLLCEDFQKFDRYVAQFKFVWENRIQSQEELDAVKATAKSEQDALTA
                     QRRNLYRKRSVAKKDRDENQAAALSAEIAALSAQIRARRREVMLCGWISEDEKRLRAQ
                     LAEAERAERQEQERQQEKSNRQRGYAR"
     gene            complement(1821..2129)
                     /locus_tag="ACZPFK_05310"
     CDS             complement(1821..2129)
                     /locus_tag="ACZPFK_05310"
                     /inference="COORDINATES: protein motif:HMM:NF046164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIR1405761.1"
                     /translation="MRNRNHIVVVHLNDKEYDRLNQKCRQAGLRREQFARAAFEGINI
                     RPRPPDTYKELAREIAAVGNNLNQIAHRVNSTGYVTQAQFEQVMDIMSRIWKLVQERA
                     "
     gene            complement(2279..3028)
                     /locus_tag="ACZPFK_05315"
     CDS             complement(2279..3028)
                     /locus_tag="ACZPFK_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase domain-containing protein"
                     /protein_id="HIR1405762.1"
                     /translation="MIRSFARGVLIGTACICLLALGWLFLFRPSLAQYEASELKEAFT
                     ESDPGGNSGVLSLPWFNAENEKQLDLASLQAQYPDVQAWLSIPGTRVDYPVLQSSMEN
                     PEYYLRRNYKGEWRTAGSLFLQSDCRLSGKTLIVYAHNMNDGSMFGCLPRYLGSGYCS
                     KHSQIQLQTPDGTWQYTVVAALETDVSQVPFNRTLFSDDADFLAFAQSLLDGAAMKTG
                     VPITADSHLLVMVTCSYSWPDARYVIVAVKS"
     gene            complement(3025..>4378)
                     /locus_tag="ACZPFK_05320"
     CDS             complement(3025..>4378)
                     /locus_tag="ACZPFK_05320"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR1405763.1"
                     /translation="YAKGDVVAVVRTGDGTSDLAAFAPGRTQATYDFLTVTHSEIGEV
                     TLTLPLGSYRVEETGAPYGFVGTTQSYDVAFGWNSQLNETVLAKEITSHNADGTDSTS
                     KFTIVDAKAADTALIERQVLGFRNERERTEAKVTKLDSKTDTPLTGAVFALFASDDVY
                     SVDGKLLVRAGEQIAVSAPSDAQGLAAFDCGLPIRGELYGTEGIYVAENEFGTGTDAR
                     FNSGLYEIRELTPPIGYYLNDEPMEVSFLYDGQPVQTLEVTCKNDGTSVLISKRKLTG
                     SDELPGATLRIVDQDGKVIREWVSGDKATEIRGLELDKPYTLVEITAPQGFAVAESIS
                     FKLVQRVDEDGSPLHENDVYVCTGKDWLVLDHWTLMEDGTVVMRDAPAPEQPRKPSEP
                     EKPKPTPVPQPIPQTGDLPWLPAVLILGLLASVIGIAAYLWHETSEDEKVAAEQWEDE
                     "
BASE COUNT          828 a         1330 c         1182 g         1038 t
ORIGIN      
        1 ccaccctcct tttcggcgct ggatagccca tgcgctcaaa aatgcgattg agcttggaca
       61 catcctccgc gcgggccagc acgtcgatca actcaccctc gctgctctta tccttgatgg
      121 ccgaaaacag tacaccgtag tgcttggcct gccgcttaaa ctcggccaga tcgcccgcct
      181 ggatgggaaa caccttgagt tccttgccgt cccgcaagag gcggctcagc cgggtacggc
      241 cccgcacctt gggattatcc cttgccagcg ccagcgcgat ggccgcaagg tttttggcac
      301 cgagcgcgct caatttgacc gccgcctcgg tgatctggat gccttcgcgc accatctggt
      361 ctgccgcttc actgccactg ttcatcttgc ataccccctt tgtctgttcg atttttcctg
      421 ctgccgttcc tgctcctgcc gctcggcgcg ttcagcctcg gccaactgcg cgcgcagtcg
      481 cttctcgtcc tcgctgatcc agccgcacag catcacctcc ctgcggcggg cgcggatctg
      541 cgcggacagg gcggcgatct cggctgacag cgccgcggct tggttttcat ctctgtcctt
      601 tttggcaaca cttctttttc ggtaaagatt cctgcgctgg gcggtaagcg cgtcctgctc
      661 ggatttggcc gtcgctttca ccgcgtccaa ttcctcctgt gattggatgc ggttttccca
      721 cacaaatttg aattgggcaa cataccggtc aaacttttga aaatcctcgc acagcaaaaa
      781 gtagcagcgg cgcgaggtgc gccgctgccg tgttttgccc agcagataga catagcgata
      841 atagagagcc ataaagccgg tgtattttct acgaggcacg accggcaatg cgccgcgcag
      901 tctcaccctg cgaacccggc gtgccggcac ctctggaatt ggcttatcgc ggatggatct
      961 ggttcgggct gcgtcctctg ccgcgcgggg cagcttcacc tcctcgcccc gcgcacggcg
     1021 ctccaacaga tttcggatag atgcctcatc gtacccctcg cccagcgatt ttagccggat
     1081 gttccggctg ccggtcttgt ggcgcagggt ggcgtatttg atattggggc cataccttac
     1141 ggaatatccc attttccgca accctgccac aaaggtttcc cagcttacgg cccttgccag
     1201 cgccctgttc acatccgcgc ggatcataga gcgggcggtt ggtttgtcct ccttttctgc
     1261 ctgtcgttcg atgtacgctt tgcccttttg cttcggatca atgattgata gtccacgctc
     1321 ccggcacaac tgatcagata ccgcccggat gcccttgaaa tatcctttga aatcgttacg
     1381 gaacatcttg ccatccacaa acgagacgga gttgaacacg atgtgattgt ggagcgtccc
     1441 ggtgttcaga tgggtgctga ctgtggcttc ataccgttca gcaaatgcgc gccgggcaaa
     1501 ctcgcatccg atctcatgcg cctgcttcgg cgtgacctcg ccgggggcaa aggattggat
     1561 gacgtggtat cctaatacat gattctctcc acctttcccc caaatagttt ccgtgcgcag
     1621 cattacctcg caggcgtggg cagggtcaca gttaaagccg gtcacaaaga gctgttgttc
     1681 tgtcttatcg ctattgtgcg cataggtaag cgcgtcttgt aagtcttgcc ccgtttttgt
     1741 ttttgcggga ttggctgcat agtcaaggca ccgtttcaaa tggttcttga ccgctttgat
     1801 ttttgtgtag gccattgttg tcacgcacgc tcctgcacaa gtttccatat tcgggacatg
     1861 atgtccatca cctgttcaaa ctgcgcttgc gttacatatc ctgtgctgtt cactctgtgc
     1921 gcaatttgat tcaggttgtt tcccaccgcc gcaatctccc gcgcgagttc cttgtaggtg
     1981 tcgggtggac ggggccgtat gtttatcccc tcaaatgccg cccgcgcaaa ctgctcgcgg
     2041 cgaagccctg cctgtcggca tttttgattc agcctgtcat attctttatc gttcagatga
     2101 acgacaacaa tatggtttcg attgcgcatc cgtcctcctt ttcaatgtat ctgcacctct
     2161 tggcgcaggg ggtttcaggg gctctgcccc tgacaagttg acatttggta tatcaaatgt
     2221 cgtgcttgct tgtactgtgc gcagcacagt ctcattcggc tgaaaacagt tgctcccgct
     2281 atgatttcac agccacaatg acatatcggg cgtcgggcca ggaatacgag caggtgacca
     2341 tcacaagcaa atgactatct gcggtgatgg gcacccctgt tttcatggcc gccccatcca
     2401 gcaggctttg ggcaaaagcc agaaaatccg catcgtccga aaacaacgtg cggttgaaag
     2461 gcacctggct gacatctgtt tccagcgccg ccacaaccgt atactgccaa gtaccatccg
     2521 gcgtttgcag ttggatttgg ctgtgcttgc tgcaatagcc gctgccgaga taccggggca
     2581 ggcagccgaa catactgccg tcgttcatgt tgtgagcata cacgataagc gttttacctg
     2641 acaggcggca gtcgctttgc aggaacaaac tgcccgcggt gcgccactcc cccttgtaat
     2701 ttctgcgaag ataatactcc gggttttcca tgctgctctg tagcacgggg taatcaacgc
     2761 gggtgccggg gattgaaagc catgcctgca catccgggta ttgggcttgc agagaggcaa
     2821 ggtcaagctg cttctcattt tcagcattga accagggcag agaaaggacc ccggagttgc
     2881 ctccggggtc gctctctgta aatgcctctt tcagctcgga tgcctcatat tgcgcaaggc
     2941 tgggccggaa aagaaacagc caaccaagcg ccagcaaaca gatgcaggcc gtcccgatca
     3001 ggacgccccg tgcaaatgaa cggatcattc gtcctcccac tgctctgccg ccactttttc
     3061 gtcctccgag gtctcgtgcc agagataggc cgcgatgcca atgaccgatg ccaacaggcc
     3121 gaggatcagc acagcaggca gccaaggcag gtcgccggtc tgcgggattg gctgaggcac
     3181 gggggtgggc ttgggcttct ccggctcgga tggcttgcgg ggctgttccg gggcgggcgc
     3241 atcgcgcatg accaccgtcc cgtcctccat tagtgtccag tggtcgagga cgagccagtc
     3301 tttaccagtg cagacataca catcgttctc atgcagaggg gagccgtcct cgtccacgcg
     3361 ctgcaccagc ttgaagctga tgctctctgc aacggcgaat ccctgcggtg cggtgatctc
     3421 caccagcgtg tagggtttat ccaattccag cccacggatt tcggtggcct tgtcgccgct
     3481 cacccattcc cggataacct tgccatcctg atccacaatg cgcagggttg cgccgggcag
     3541 ctcatcgctg ccggtcagct tgcgcttgga gatcagcacc gaagtgccat cgtttttgca
     3601 ggtaacctcc agcgtttgaa ccggctggcc gtcatagagg aaagatacct ccatcggctc
     3661 atcgttcagg taatagccta tcggcggggt caattctcga atctcataca ggccactgtt
     3721 gaaacgggca tccgtcccgg tgccgaactc attttccgcc acatagatgc cctcagtgcc
     3781 atacagttca ccccggatgg gcaggccgca gtcaaaagcg gcaaggccct gcgcgtcgga
     3841 cggcgcagat accgcgatct gctcccccgc acggacaagc agtttgccat ccacgctgta
     3901 aacatcatcg ctggcaaaca gggcaaagac ggcaccggtt agcggggtat ctgtcttgct
     3961 gtccagcttg gttaccttgg cctcggtccg ctcccgctca ttgcggaagc caagtacctg
     4021 ccgctcgatc agagcggtgt cggcagcctt ggcatcgaca atcgtgaact tggaagtgct
     4081 gtccgtaccg tctgcgttat ggctggtgat ctcctttgcc agcacggttt cgttaagctg
     4141 gctgttccat ccaaaggcca catcatagct ttgggtcgta ccgacaaacc cgtatggggc
     4201 accagtttcc tcgacacgat agctgcccag cggcagggtc aatgtcacct cgccaatctc
     4261 gctatgggtt acggtcagga agtcataggt ggcctgcgtc cggcccggcg caaaggcggc
     4321 aagatcgctg gtgccatcgc cggtacgcac gactgccacc acatcgccct tggcatac
//
LOCUS       DBMDYA010000165         4375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4796_length_4375_cov_1.707768, whole genome shotgun sequence.
ACCESSION   DBMDYA010000165 DBMDYA010000000
VERSION     DBMDYA010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_4375_cov_1.707768"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /gene="rpoC"
                     /locus_tag="ACZPFK_05325"
     CDS             complement(<1..2151)
                     /gene="rpoC"
                     /locus_tag="ACZPFK_05325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1405764.1"
                     /translation="MEFNVFDSIKIGLASPDQIRAWSYGEVKKPETINYRTLKPERDG
                     LFCERIFGPTKDWECHCGKYKRIRYKGKICDRCGVEVTRSKVRRERMGHIELAAPVSH
                     IWYFKGIPSRIGLMLDLSPRLLEKVLYFASYIVTDPGYTPLEEKQLLSEKEYRDMRDR
                     YEDEFKASMGAEAIKELLSRIDLDKLSEELHADLEDASGQKRVRILKRLEVVEAFRLS
                     GNRPEWMIMDVVPVIPPDIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGAP
                     DIIVRNEKRMLQEAVDALIDNGRRGRPVTGPNNRPLKSLSDMLKGKQGRFRQNLLGKR
                     VDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKDLVENGVANNIKSARKMVERV
                     HPQVWDSLETVIKGHPVLLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLVCTAFNADFD
                     GDQMAVHLPLSAEAQKEAKTLMLASHNLLKPSDGKPVAVPTQDMVLGSYYLTMVNDGD
                     KGEGKVFRDEAEAMMAYQEGVITLQAKIKVRRTLEIDGELKSRIIDTTIGRIIFNNPI
                     PQDLGYVDRSSEENLFKLEVDFLVTKKTLGQIIDRCIHIHGTERTSVMLDAIKAQGYK
                     YSTKGAITVSVSDAVIPPKKAELLTAAEERINKIHKQYNKGLISDDERYNSIINIWNK
                     TTDDVSAAVTENLSDRNPIFMMADSGARGSMNQIR"
     gene            complement(2234..>4375)
                     /gene="rpoB"
                     /locus_tag="ACZPFK_05330"
     CDS             complement(2234..>4375)
                     /gene="rpoB"
                     /locus_tag="ACZPFK_05330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1405765.1"
                     /translation="YTHYGRMCPIETPEGPNIGLISYLATFAKINEYGFIEAPYRRID
                     KETHRVTDEVVYMTADVEDNYVVAQANEPLDENGWFVNERVNARCRDEILSVERERAD
                     FMDVSPKMVVSVATSMIPFLENDDANRALMGSNMQRQAVPLLVTEQPIVATGMEYKAA
                     CDSGVCVLAKHDGVVEHVDADKVVLRTGKNTTEEYDIIKFMRSNQGTCVNQRPIVEKG
                     DQIKAGQVLADGPATKNGEVSLGKNALIGFMTWEGYNYEDAVLINEKLVREDVYTSIH
                     IEEYELEARETKLGPEEITRDIPNVGDDALKDPDERGIIRVGAEVNAGDILVGKVTPK
                     GETELTAEERLLRAIFGEKAREVRDTSLRVPHGEYGIIVDVKVFTPDNCDELNPGVRE
                     VVRCYIAQKRKLSVGDKMAGRHGNKGVVSRILPQEDMPFLPDGTPLDICLNPLGVPSR
                     MNIGQVLEVHLGMAAKALGWKVATPVFDGAHEQDIREMFKQAGLREDGKTYVYDGRTG
                     ERFDNPVTVGYMYFLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGAAYTLQEILTVKSDDVVGRVKTYEAIVKGENVPRPGIPESFRVLIKELQSLG
                     LDVRVLDEEKNEIDLKQNFDDDDVGFDSSSLPLGDDVLVESELDNYTIGDVEEGEAPV
                     AEEDEDSDELIETDDLFSDLETEPESDESNE"
BASE COUNT          685 a         1357 c         1411 g          922 t
ORIGIN      
        1 acggatctgg ttcatcgagc cgcgggcgcc ggaatccgcc atcataaaga tggggttgcg
       61 gtcggacagg ttctcggtga cggccgccga cacgtcgtcg gtggtcttgt tccagatgtt
      121 gatgatgctg ttatagcgct cgtcgtcgga gatcaggccc ttgttgtact gcttgtgaat
      181 cttgttgatg cgctcctcgg cggcggtcag cagctcggct ttcttgggcg ggatgacggc
      241 gtcggagacc gagaccgtga tggcgccctt ggtggaatat ttgtaaccct gcgccttgat
      301 ggcgtccagc atcaccgagg tgcgctcggt gccgtggatg tggatgcagc ggtcgataat
      361 ctggcccagc gtcttcttgg tcaccaggaa gtcgacctcc agcttgaaga ggttctcctc
      421 gctgctgcgg tccacatagc caaggtcctg cgggatggga ttgttgaaga tgatgcggcc
      481 gatggtggtg tcgatgatgc ggctcttcag ctcgccgtcg atctccagcg tgcggcgcac
      541 cttgatcttg gcctgcaggg tgatgacgcc ctcctgatag gccatcatgg cctcggcctc
      601 gtcgcggaac accttgccct cgcccttgtc gccgtcgttg accatggtca ggtaataact
      661 gcccagcacc atgtcctggg tgggcaccgc caccggcttg ccgtcggagg gcttgagcag
      721 gttgtgggag gccagcatca gggtcttggc ctctttctgc gcctcggcgg acagcggcag
      781 atgcaccgcc atctggtcgc cgtcaaagtc agcgttgaac gcggtgcaga ccagcgggtg
      841 cagcttgatg gcacggccct cgaccagcac cggctcaaag gcctggatgc ccaggcggtg
      901 cagggtcggg gcgcggttca gaagcaccgg gtgccccttg atgaccgtct cgagggaatc
      961 ccacacctgg ggatggaccc gctccaccat tttgcgggca gatttgatgt tgttggcgac
     1021 cccgttttcc accaggtcct tcatcacaaa aggcttgaac agctccagcg ccatctcctt
     1081 gggcaggccg cactggtaca tcttcagctc cgggccgacg acgatgaccg aacggccgga
     1141 atagtccacg cgcttgccca gcaggttctg gcggaagcgg ccctgcttgc ccttgagcat
     1201 atcggacaag ctcttgaggg gacggttgtt ggggccggtg accggacggc cgcggcggcc
     1261 gttgtcgatc agggcgtcca ccgcctcctg cagcatccgc ttctcgttgc gcacgatgat
     1321 gtcgggcgcg cccagctcca gcaggcgctt gaggcggttg ttgcggttga tgacacggcg
     1381 gtacaggtcg ttcaggtcgg aggtggcgaa acggccgccg tccagctgca ccatggggcg
     1441 gatgtcgggc gggatgaccg gcaccacgtc catgatcatc cactcggggc ggttgccgga
     1501 aaggcggaat gcctccacga cctccagccg tttgaggatg cgcacccgct tctggcccga
     1561 ggcgtcctcc aggtcggcgt gcagctcttc cgagagctta tccaggtcga tgcggctcag
     1621 cagctccttg atggcctcgg cgcccatgga cgccttgaac tcatcctcgt agcggtcccg
     1681 catgtcgcga tactcttttt cgctgagcag ctgcttttcc tccagcgggg tgtagcccgg
     1741 gtcggtgacg atatagctgg caaagtacag cactttttcc agcaggcggg gcgagaggtc
     1801 cagcatcagg ccgatgcggg aggggatgcc cttgaaatac cagatatggc tgaccggcgc
     1861 ggccagctcg atgtggccca tccgctcgcg gcggaccttg gagcgggtga cctcgacgcc
     1921 gcagcggtcg cagattttgc ctttatagcg gatgcgcttg tacttgccgc agtggcactc
     1981 ccagtccttg gtcggtccga agatgcgctc gcagaacagg ccgtcgcgct ccggcttgag
     2041 ggtgcggtag ttgatcgttt cgggcttttt tacctcgccg tagctccagg cgcggatctg
     2101 gtcgggggag gcaagaccga ttttgatcga atcaaagacg ttaaactcca ttgagcgaat
     2161 ccttccttta ctctacaaat tgccggacgg ccggggagcg ggacgcaagg gcccgcgggc
     2221 cggcctcact tcattattcg ttcgattcgt cgctttccgg ctcggtctcc agatccgaga
     2281 agaggtcgtc ggtctcgatc agctcatccg agtcctcatc ctcttccgca acgggagctt
     2341 cgccctcttc gacgtcgcca atggtgtagt tgtccaactc gctctcgacc agcacatcgt
     2401 cgcccagcgg cagcgaggag gaatcgaacc cgacgtcgtc atcgtcgaag ttctgtttca
     2461 gatcgatctc gtttttctct tcgtccagca cgcgcacgtc cagccccagg gactgcagct
     2521 ctttgatgag cacgcggaac gattcgggga tgcccgggcg cggcacattc tcgcccttga
     2581 cgatggcctc ataggtcttg acacggccca ccacgtcgtc ggacttgacg gtcaggatct
     2641 cctgcagcgt gtaagccgcg ccgtaggcct ccagggccca gacctccatc tcgccgaagc
     2701 gctggccgcc gaactgggcc ttgccgccca ggggctgctg tgtgaccagg ctgtacgggc
     2761 cggtggagcg ggcgtggatc ttgtcgtcga ccaggtgatg cagcttgagg aagtacatat
     2821 agcccacggt gaccgggttg tcgaagcgct cgccggtgcg gccgtcatag acataggtct
     2881 tgccgtcctc tcgcaggccc gcctgcttaa acatctcgcg gatgtcctgc tcgtgcgcgc
     2941 cgtcaaaaac gggggtggcg actttccagc ccagggcctt ggcggccata cccaggtgga
     3001 cctccagcac ctgcccgatg ttcatacggg agggcacgcc cagcgggttc aggcagatgt
     3061 ccagcggcgt tccgtcgggc aggaacggca tatcctcctg cggcaggatg cgggacacga
     3121 cgcccttgtt gccgtggcgg cccgccatct tgtcgcccac gctcagcttg cgcttttggg
     3181 cgatatagca gcgcacgacc tcgcgcacgc cggggttcag ctcgtcgcag ttgtcgggcg
     3241 tgaagacctt tacgtccacg atgatgccgt actcgccgtg gggcacccgc agcgaggtgt
     3301 cgcgcacctc gcgcgctttt tcgccgaaga tggcgcgcag cagccgctcc tcggcggtca
     3361 gctcggtctc gcccttgggg gtcaccttgc ccaccaggat atcgccggcg ttgacctcgg
     3421 cgcccacgcg gatgatgccg cgctcgtccg ggtctttcag ggcgtcgtcg cccacgttgg
     3481 ggatgtcgcg ggtgatctcc tcggggccca gcttggtctc gcgggcctcc agctcatact
     3541 cctcgatgtg gatcgaggtg tacacgtcct cgcgcaccag tttttcgttg atgaggaccg
     3601 cgtcctcgta gttgtagccc tcccaggtca tgaaaccgat cagggcgttc ttgcccaggc
     3661 tgacctcgcc gtttttggtg gcggggccgt cggccagcac ctggcccgcc ttgatctggt
     3721 cgcctttttc cacaatgggc cgctggttga cgcaggtgcc ctggttggag cgcatgaact
     3781 tgatgatgtc gtattcctcg gtggtgttct tgccggtgcg cagcacaacc ttgtcggcgt
     3841 ccacgtgctc caccacgccg tcgtgcttgg ccagcacgca cacgccggag tcgcaggcag
     3901 ccttgtactc catgccggtg gcgacgatgg gctgctcggt gaccagcaga ggcaccgcct
     3961 gccgctgcat gttggagccc atcagcgcgc ggttggcgtc gtcgttctcg aggaacggga
     4021 tcatcgaggt cgccaccgag acgaccatct tcggggatac gtccatgaag tcggcgcgct
     4081 cgcgctccac cgacaaaatc tcatcgcggc agcgggcgtt gacgcgctcg ttgacgaacc
     4141 agccgttttc gtccagcggc tcgttggcct gggcgacgac atagttgtcc tccacatcgg
     4201 cggtcatgta gacgacctcg tcggtgaccc ggtgcgtctc cttgtcgatg cggcggtagg
     4261 gcgcctcgat aaagccgtac tcattgattt tggcaaaggt ggccagatac gagatcagac
     4321 cgatgttggg gccctcaggg gtctcaatgg ggcacatgcg gccgtagtgg gtgta
//
LOCUS       DBMDYA010000166         4365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4805_length_4365_cov_1.484756, whole genome shotgun sequence.
ACCESSION   DBMDYA010000166 DBMDYA010000000
VERSION     DBMDYA010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_4365_cov_1.484756"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            249..1292
                     /locus_tag="ACZPFK_05335"
     CDS             249..1292
                     /locus_tag="ACZPFK_05335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR1405766.1"
                     /translation="MAKTMRSIAAVGGGKVQIVRDVPMPVPGPYESLVKVRSCGFCSG
                     TDFHIIRGEMAVPVDPFPTLLGHEGIGDIVETGAKVRYHKVGDRFMNPVFKKMPGLQY
                     GVTWGAMSDYCIVQDQRAMREDGLPDSAFNPSQNACRQIPNDLDPIDMGVLLTLNECF
                     SAARNFTPEGKDILVYGAGPMGLATMKYLRYLGAKTITCVDCIDERLALAKSLSGADE
                     VINFATQDKDALLEGRLFDRVIDIVGLSSLLMEGSHRLRPYGIVGSMGVLRNTDAVLD
                     VTRLKNNTLLQMLNFPYGQYDITAENVALIRSGFVNPKDFYTHVRKVEQIDEVLELVR
                     SKQALKVVLDFDD"
     gene            complement(1494..2768)
                     /locus_tag="ACZPFK_05340"
     CDS             complement(1494..2768)
                     /locus_tag="ACZPFK_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405767.1"
                     /translation="MKGRGKLHRAWWMMVGCILLQAGATGVFSNCATIFYSSICGELG
                     FSNGAMAMFTTVRTVTMAFAMTGVTLLYKKVDLRVLLSVLSVLGAACFASQALFHNIW
                     QWYVMGAIYGVASGGLMTVPATIVINNWFHERTGFALGLALSASGVAGAVINPLCSAL
                     ISSLGWRPAAVAVAGLALALLLPATAFILRLTPEEVGCTPYGAKQQNGVAERTVLRAE
                     NSPLAAKGGVFALCFILVILPNCVVQITYHISLFVQQEGLSLALAATLTSLVMVGNTL
                     GKLLLGMLNDRKGPWFTSCCACAALALASLGFTGGRAAPWLLYLSAPLLGVSFSLAAM
                     LPSLIARQIYAGQYQKRYSVLMTTGTLLGAVANTAIGVASDVAGSYLPVYGVSAAACA
                     ACAALSVLLGAWERKAGPAALEKAVVPEGENG"
     gene            complement(2872..4164)
                     /locus_tag="ACZPFK_05345"
     CDS             complement(2872..4164)
                     /locus_tag="ACZPFK_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405768.1"
                     /translation="MKLDNKRTILVGLAFMSISAFWQVYDGIVPLILKNTFGIGDTAS
                     GVVMALDNVLALFLLPVFGSLSDRTHTRLGRRMPYIVCGTAAVAVAIILIPLGDHLYS
                     LPLFVVGLGLALLAMSSYRSPAVALMPDVTPKPLRSKGNAIINLMGAVGGILMLLTIK
                     LIVPKTGHPDYIPLFVILDVFMVACVAVLFFTVNEPKTVKKMEAESRAMGVSEQDDEP
                     QQGHAGEKLPRDVFRSLCFLLASIFLWFMGYNAITTAFSKYANHMWGLEGGDYAMVLM
                     VAQAAAIVAFIPVGIIASKIGRKRTILFGVAFLAVAFGSGIFFSDHFTWLMFVFFSLA
                     GVGWAFINVNSYPMVVEMSKGGDVGKYTGYYYTFSMAAQIVTPILSGAVMQFLGYKYL
                     FPYGTLFVVLSFVTMLFVRHGDNRPKAQVGIEAFDVDD"
BASE COUNT          833 a         1443 c         1377 g          712 t
ORIGIN      
        1 aggggctcgt ctgagctccg gcatcggcgc tcccccgggc gcgcgggagg ggggcggtcc
       61 tttcccgacg cgggaggcgt cttaaaagac ctctgcgccc tctcctcccc gctctctgca
      121 agagcccggc cttttcacgc cggaaagggc gcggcgtgcc gtcctgcggc cgccgtcccg
      181 gcgccttacc aaggggcgtg ttccctttcc gatctatttt cacaaaaaca cacggaggca
      241 atgtaaaaat ggccaagacc atgcgttcca tcgccgcagt gggcggcggc aaggtacaga
      301 tcgtgcggga cgtgcccatg cccgtgcccg gcccttacga gagcctggta aaggtgcgca
      361 gctgcggctt ttgcagcggc acggattttc atatcatccg cggcgagatg gccgtgccgg
      421 tggacccgtt ccccaccctg ctggggcacg aggggatcgg cgatatcgtg gagaccggcg
      481 ccaaggtgcg ctatcacaag gtgggcgacc gcttcatgaa cccggtcttc aaaaaaatgc
      541 cgggcctgca atacggggtg acctggggcg cgatgtcgga ctattgcatt gtgcaggatc
      601 agcgcgccat gcgggaggac ggcctgcccg acagcgcgtt caacccctcg cagaacgcct
      661 gccgccagat ccccaacgac cttgacccca ttgacatggg ggtgctgctg accctgaacg
      721 agtgcttctc cgcggcgcgg aatttcaccc ccgagggcaa ggacatcctc gtctacgggg
      781 cggggccgat ggggcttgcc accatgaagt acctgcgcta cctgggggcc aagaccatta
      841 cctgtgtgga ctgcatcgac gagcggctgg cgctggcaaa gtcgctctcc ggcgcggacg
      901 aggtcatcaa cttcgccacc caggacaagg acgccctgct ggaggggcgc ctcttcgacc
      961 gggtcattga tatcgtcggc ctctcctccc tgctgatgga gggctcgcac cggctgcggc
     1021 cctacggcat cgtgggctcg atgggggtgc tgcgcaatac cgacgcggtg ctggatgtca
     1081 cccggctcaa aaacaacacc ctgctgcaga tgctcaactt cccctacggc cagtacgaca
     1141 tcaccgcaga aaacgtggcc ctgatccgca gcggcttcgt caaccccaag gatttctaca
     1201 cccatgtgcg caaggtggag cagatcgacg aggtgttgga gctggtgcgc agcaagcagg
     1261 cgctgaaagt ggtgctggat ttcgacgact gagcgggcgc gaacgccggg cctctcgcct
     1321 cccgcacagg gagcgcggcg ctccgccggg cccggggcgg gcgcggtaag ccccgcgccg
     1381 cccggtttaa aacgagatca gcccagatcc tctcccctca aagaaaccca agggccggct
     1441 tgcgccggcc cttgggtttc ttattttctt tttccagcag tacagcgtcg gggtcagccg
     1501 ttttctccct ccggcacgac cgccttttcc agcgccgcgg ggcccgcttt gcgctcccag
     1561 gcgcccagca gcaccgagag cgccgcacag gccgcgcaag ccgcggcgct gaccccgtag
     1621 accggcaggt aactgcccgc cacgtccgag gccaccccga tggccgtatt ggccaccgcg
     1681 cccagcaggg tgccggtggt catcagcacg ctgtagcgct tttggtactg gcccgcatag
     1741 atctgccggg cgatcagcga gggcagcatg gccgccagcg aaaacgagac gcccagcagc
     1801 ggggcgctga gatagagcag ccagggcgcg gcccggccgc cggtgaagcc cagcgaggcc
     1861 agcgccagcg ccgcgcaggc gcagcagctg gtgaaccagg ggcccttgcg gtcgttgagc
     1921 atgcccagca gcagcttgcc cagcgtgttg cccaccatca ccagcgaggt gagggtggcg
     1981 gccagcgcca ggctgaggcc ctcctgctgc acgaacagcg agatgtgata ggtgatctgc
     2041 accacgcagt tgggcaggat gaccaggatg aagcagagcg cgaagacccc tcccttggcc
     2101 gccagggggc tgttctcagc ccgcagcacc gtgcgctcgg caacgccgtt ttgctgcttt
     2161 gcgccgtagg gggtgcagcc cacctcctcc ggcgtgagcc gcaggataaa cgccgtggcg
     2221 ggcagcagca gcgccagggc cagcccggcc acggcgaccg cggcgggccg ccagcccagc
     2281 gagctgatga gcgccgagca gagcgggttg atgaccgcgc ccgccacgcc ggaggcggag
     2341 agcgccaacc ccagcgcaaa gccggtgcgc tcgtggaacc agttgttgat gacgatggtg
     2401 gcgggcacgg tcatcaggcc gccgctggcc acgccgtaaa tcgcccccat cacataccac
     2461 tgccagatgt tgtgaaacag tgcctgcgag gcaaagcagg ccgcgcccaa caccgacagc
     2521 accgagagca gcacccgcag gtcgaccttt ttgtacagca gcgtgacgcc ggtcatggca
     2581 aaggccatgg tcacggtgcg cacggtggtg aacatcgcca tcgccccgtt gctgaagccc
     2641 agctccccgc agatggacga ataaaaaatc gtcgcgcagt tgctgaacac cccggtggcg
     2701 cccgcctgca gcaggatgca gcccaccatc atccaccacg cccggtgcag ttttcctctt
     2761 cccttcaaaa cgaacccgct cctttacatt ttacttccca tacttcctta cacgacgcgg
     2821 cgcgccgccc cagccgggct gcgcgccgct ctccgccctg ccgcggggcg gtcagtcgtc
     2881 cacgtcaaag gcctcgatgc ccacctgcgc cttgggccgg ttgtcgccgt gccggacgaa
     2941 cagcatcgtc acaaagctca gcaccacgaa cagcgtgccg taggggaaca ggtatttgta
     3001 acccaaaaac tgcatcaccg cgcccgagag gatgggggtg acgatctggg cggccatcga
     3061 gaaagtgtag tagtagccgg tgtatttgcc cacgtcgccg cccttgctca tctccaccac
     3121 catcgggtag ctgttgacgt tgataaaggc ccagccgacg ccggccagcg agaagaacac
     3181 aaacatcagc caggtgaaat ggtcgctgaa aaagatgccc gagccgaacg ccaccgccaa
     3241 aaaggcgacg ccgaacagga tggtgcgctt gcgcccgatc ttgctggcga tgatgcccac
     3301 cgggataaag gccacgatgg ccgccgcctg ggccaccatc agcaccatgg cgtagtcgcc
     3361 gccctccagg ccccacatgt ggttggcgta tttgctgaac gccgtggtga tggcgttata
     3421 gcccatgaac cacagaaaga tgctggccag caaaaagcag agggagcgga aaacgtctct
     3481 gggcagcttt tcgcccgcgt ggccctgctg gggctcgtcg tcctgctcgc tcacgcccat
     3541 ggcgcggctc tcggcctcca tctttttcac cgtcttgggc tcgttgacgg tgaagaacag
     3601 caccgccacg caggccacca taaacacgtc caggatgacg aagagcggga tgtaatccgg
     3661 atggccggtc ttggggacga tgagcttgat ggtgagcagc atcaggatgc cgcccaccgc
     3721 gcccatcagg ttgatgatgg cgttgccctt gctgcgcagg ggcttggggg tgacgtccgg
     3781 catcagggcc acggccgggc tgcggtagct ggacatggcc agcagcgcca ggcccagacc
     3841 caccacaaac agcggcaggc tgtacaggtg gtcgcccagc gggatcagga tgatggccac
     3901 cgccaccgcc gcggtgccgc agacgatata gggcatgcgc cggcccagcc gggtgtgggt
     3961 gcggtcgctg agcgagccga agaccggcag caaaaacagg gccagcacgt tgtccagcgc
     4021 catgacgacg ccggaggccg tgtccccgat gccgaaggtg tttttcaaaa tcagggggac
     4081 gatgccgtcg tacacctgcc agaacgcgct gatggacata aaggcaaggc cgaccaggat
     4141 ggtgcgcttg ttgtcgagtt tcatcaggat attcctccgc atgtttgatg cggggcagcc
     4201 cgccccgccc tctcgcgatc tatataaggt attgtaacat tttgcccgcc ccgattcaat
     4261 catgctgcga cattctattg catccgcaag gacctgcccc agctcccagt cggccagggt
     4321 gtcaaggaca taaacataaa ccattaacat aaaattcctc cgttt
//
LOCUS       DBMDYA010000167         4361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4811_length_4361_cov_1.468779, whole genome shotgun sequence.
ACCESSION   DBMDYA010000167 DBMDYA010000000
VERSION     DBMDYA010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_4361_cov_1.468779"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="ACZPFK_05350"
     CDS             <1..790
                     /locus_tag="ACZPFK_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405769.1"
                     /translation="ILLYVFYSRMVYCQTSELSAENDSLTRLLHDNSRRSLERAAEID
                     AASPKAEVSALAELNAVNHSYDPAVEENPSDYIDSTGMPLIDEMLQNYYLYACRRGID
                     LRVYVHDPLTLLFSDYTPYWKQISDTIGNLVANAITAVEKSHAERPEVFCAFGVVAGK
                     HYEIHIFDSGLDFPAKVLERLGEEGNTTGGSGIGMMSILEASRVAKFSLRIQEGEPCY
                     PYTKKIFVVFDGEGRIEIETWRAAQLHVHNPRITVKQLGGELHV"
     gene            774..1001
                     /locus_tag="ACZPFK_05355"
     CDS             774..1001
                     /locus_tag="ACZPFK_05355"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1405770.1"
                     /translation="MSSTFNLVEDYFEDENGHRILAYSICSAAHGFYLSCVSSDRAVV
                     ERLVQMLNSGNISPCHLHDIIEDYISALYIA"
     gene            complement(1050..2576)
                     /gene="guaA"
                     /locus_tag="ACZPFK_05360"
     CDS             complement(1050..2576)
                     /gene="guaA"
                     /locus_tag="ACZPFK_05360"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1405771.1"
                     /translation="MSETVIIVDFGGQYNQLIARRVRENNVYSEVVPYGKALAEIKAK
                     QPKGIIFTGGPASVYEEGAPTVDPEVFQLGIPVLGICYGAQLMAHLLGGRVVPPVARE
                     YGRVRIQSNGGLMAKVVREGTSWMSHGDQIETIPQGFAVTATSNTCPVAAMENAAAGL
                     YAVQFHPEVQHTAFGRELLHAFLYDVCGCKGDWTSAGFVERAVAEVRAEVGEERRVLC
                     ALSGGVDSTVAAVLVHRAIGDRLTCIFVDHGLLRKNEGDQVMDTLTREFDLNVIRVNA
                     RELFLSKLAGVTEPEAKRKIIGEEFIRVFDAEAAKLGKMDYLLQGTIYPDVIESGAGD
                     AAVIKSHHNVGGLPEDVQFKLLEPLRLLFKDEVRSAGRELGLPEHIVNRQPFPGPGLA
                     IRCLGEVTEEKLAIVRESDAILREEIARAKLDRAIWQYFTVLPNIRSVGVMGDGRTYS
                     HAVAIRAVTSVDGMTSDWARIPYEVLEKISSRIVNEVPGVNRVVYDVTTKPPATIEWE
                     "
     gene            2819..4117
                     /gene="guaA"
                     /locus_tag="ACZPFK_05365"
     CDS             2819..4117
                     /gene="guaA"
                     /locus_tag="ACZPFK_05365"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1405772.1"
                     /translation="MKQDMIAILDLGSTRNTDAARAVRALGVYSEIINYDITAEELAA
                     LPNLRGIVACGGPNNVVDGAKISLGDPIKKSGLPLFFFDCEDEAASAPMPAESANIEN
                     ALRSFVFDLCHAQANWNMENFIADQVELVRRQVGQKKVLLALSGGVDSSVVAALLLRA
                     IGDQLTCVHVNHGLLRKGEPEQVVQVFRHELGANLVYVDASDRFLDKLAGVADPEDKR
                     KIIGAEFIRVFEEEARKLDGIEFLAQGTIYPDIVESGTKTAKMVKSHHNVGGLPDDLQ
                     FSLVEPLKQLFKDEVRACGVALGLPDSMVYRQPFPGPGLGVRCLGAITRDRLELVRES
                     DAILREEFSRAGLDKTVWQYFTVVPDFKSVGVRDNARCFEYPVILRAVNTVDAMTAEV
                     PELEWKVLRRITRRIMDEVPGINRVCFDLSPKPNATIEWE"
BASE COUNT          910 a         1297 c         1227 g          927 t
ORIGIN      
        1 tatattactt tatgttttct attctaggat ggtatactgc caaacttcag aactttcagc
       61 agaaaacgat tcacttaccc gccttctaca cgataactcc cgccgttctc ttgagcgggc
      121 ggctgaaatt gacgcggcgt cgccaaaagc ggaggtctcc gcgctcgcag agttgaacgc
      181 cgtcaatcat tcttatgacc ctgccgtaga agaaaacccc agcgactata tcgattccac
      241 cggcatgccg ctgatcgatg aaatgcttca aaattattat ctttatgcct gccgccgtgg
      301 catcgatctg cgggtttatg tccacgaccc tctcacgctt ctattcagcg attacacccc
      361 atactggaag cagatcagcg acaccattgg caatttggtc gccaacgcga tcaccgccgt
      421 agaaaaatca catgcagagc gccccgaggt cttttgtgcc tttggcgttg tcgcgggcaa
      481 acactacgag attcatatct ttgattctgg tctggatttc ccggccaaag tgctggagcg
      541 gctcggcgag gagggcaaca ccaccggcgg cagcggcatc ggtatgatga gcatcctgga
      601 ggcatcccgt gtggccaagt tttctctgcg cattcaagag ggtgagccgt gttatcctta
      661 caccaaaaag atatttgtcg ttttcgacgg cgagggccgg atagaaattg agacctggcg
      721 tgcagctcag cttcacgtgc acaacccccg catcactgtc aaacagctag gaggtgagct
      781 ccacgtttaa tctggttgag gactattttg aagatgaaaa tgggcatcgg atccttgcct
      841 atagcatttg cagtgctgca catgggtttt atctgtcctg cgtcagttct gatcgtgctg
      901 tcgttgagcg cctagtgcaa atgctcaact caggaaatat atccccctgt catttgcacg
      961 acatcattga agactatatc tccgcccttt acatcgcttg aaaaaaagat gcgtgacctg
     1021 ttggtcacgc atcctttttt ctgtttaaat cattcccact cgatcgtcgc gggcggtttg
     1081 gtggtcacgt cgtagaccac gcggtttacc cccggcacct cgtttacgat gcggctgctg
     1141 attttttcca gcacctcata ggggatgcgc gcccagtcgc tggtcatgcc gtccacactg
     1201 gtaacagcac gaatggccac tgcgtggctg taagtgcggc cgtcgcccat cacgccgacg
     1261 ctgcggatat tgggcagcac ggtgaaatat tgccagatcg cgcggtccag cttcgcgcgc
     1321 gcgatttctt cccgcaaaat ggcgtccgac tcgcgcacaa tagccagctt ttcctccgtc
     1381 acctcgccca gacagcggat ggccagcccc ggcccgggga aaggttgccg gttgacgata
     1441 tgctcgggca ggcccagctc gcggcccgcg ctgcgcacct cgtctttgaa caacaggcgc
     1501 agaggctcca gcagcttgaa ctgcacgtcc tcgggcaggc cgcccacatt gtggtggctc
     1561 ttgatgaccg ccgcgtcgcc cgcgccgctc tcgatcacgt cggggtagat ggtgccctgc
     1621 agcaggtaat ccatcttgcc cagcttggcg gcctcggcgt caaagacgcg gatgaactct
     1681 tccccgatga tcttgcgctt tgcctccggc tccgtgacgc cggccagctt gctcaaaaac
     1741 agctcgcgcg cattgacccg gatgacgttc aggtcaaatt cccgggtcag cgtgtccatg
     1801 acctgatcgc cctcgttctt gcgcagcagc ccatgatcga caaaaatgca ggtcaggcgg
     1861 tcgccgatgg cccggtggac cagcaccgcc gccacagtcg aatccacgcc gccggacagc
     1921 gcgcacagaa cccgtcgctc ctcgccgacc tcggcccgca cctccgccac cgcccgctcc
     1981 acaaagccgg cgctggtcca atcaccctta caaccgcaaa cgtcatacaa aaaagcatgc
     2041 agcagctcgc ggccgaaagc ggtgtgttgt acctcgggat gaaactgcac cgcatacagg
     2101 cccgccgcgg cgttttccat cgccgccacc gggcaggtgt tgctggtggc agtcacagca
     2161 aacccctgag ggatggtctc tatctgatcc ccgtgactca tccaactggt gccctcgcgc
     2221 acgactttcg ccatcaggcc accgttggat tgaatgcgca cccggccgta ttcccgcgcc
     2281 accgggggca caacccgtcc ccccagcaga tgcgccatca gctgagcgcc gtagcagatg
     2341 cccagcaccg ggatgccgag ctgaaagacc tccgggtcca ccgtgggcgc gccctcttcg
     2401 taaacactgg ccgggccgcc ggtgaagatg atgcccttgg gctgctttgc cttgatttcc
     2461 gccagggctt tgccgtaggg cacgacctcg ctgtaaacgt tgttttcccg cacgcggcgg
     2521 gcgatgagct ggttgtactg gccgccgaaa tcgacgatga tgactgtctc cgacatggca
     2581 ttgactccgt ttcgttgatt cagtgtgaaa gctacttcgt tagtatacca tatttaccgc
     2641 aaaagcgcac aaaaaaatta cattttttgg gtctttttgc gaaaattcgg ccgtccctaa
     2701 aacagagctt ttttgttgct tgtcgagcta aaaccgctgt gctatactga aaaacagaaa
     2761 aaacgattcg cgcctttgcg gaaaaaaccg aaaagcgcag gcagaaagca ggagtggtat
     2821 gaaacaggat atgatcgcga tccttgattt gggcagcact cgcaacaccg acgcggcgcg
     2881 cgcggtgcgg gcgctcggcg tttacagtga gatcatcaac tacgacatca cggccgaaga
     2941 actggcggcg ctgcccaatc tgcgcggcat cgtggcctgc ggcgggccga acaatgtggt
     3001 ggacggcgca aaaatcagtc ttggtgaccc gatcaaaaag tccggcctgc cgctgttctt
     3061 ttttgactgc gaggacgaag ccgccagtgc accgatgccc gcggagagcg ccaacatcga
     3121 aaatgcgctg cgctcttttg tgtttgatct ctgccatgca caggcgaact ggaatatgga
     3181 aaactttatc gccgaccagg tggaactagt gcgccgccag gtggggcaaa aaaaagtgct
     3241 gctcgcgctc tcgggcggcg tggacagctc ggtagtggcg gcgctgctgc tgcgcgccat
     3301 cggcgaccag ctgacctgcg tgcatgtcaa ccacggcctg ctgcgcaagg gcgagccgga
     3361 gcaggtggtg caggtcttcc gccacgaact gggggccaac ctggtttatg tggatgccag
     3421 cgaccgcttt ttggacaagc tggcgggcgt tgccgaccct gaggacaagc gcaagatcat
     3481 cggcgcggag ttcatccgcg tctttgagga ggaagcccgc aagctggacg gcatcgaatt
     3541 tttggcgcag ggcaccatct acccggacat cgtcgaaagc ggcaccaaaa ccgccaagat
     3601 ggtcaagagc caccacaatg tgggcggtct gcccgacgat ctgcagttct cgctggtgga
     3661 gccgctcaag cagttgttca aggacgaggt gcgcgcctgc ggcgtggcgc tgggtctgcc
     3721 cgattcgatg gtctaccgcc agccgttccc cggccccggc ctgggggtgc gctgcctggg
     3781 ggcgatcacc cgcgaccggc tggagctggt gcgcgaatcc gatgccatcc tgcgggagga
     3841 attcagccgc gccgggctgg acaaaacggt gtggcagtac ttcacggtgg tgccagactt
     3901 caagtcggtg ggcgtacggg acaatgcgcg ctgctttgaa tacccggtca tcctgcgcgc
     3961 ggtcaacacc gtggacgcca tgacggccga ggtgccggaa ctggaatgga aagtgctgcg
     4021 ccgcatcacc cgccgcatca tggacgaggt gccgggcatc aaccgcgttt gctttgatct
     4081 gtcgccgaag ccgaatgcga cgatcgagtg ggaataatat cggaggcttg aaaaagccaa
     4141 taatacaggg cttttcagtg cctaactccc actgtggcaa cgattgggca acacttttca
     4201 ttactccatc aaaaatggac tatctgattt tgaaaatcag atagtccatt ttttgttatt
     4261 tatcagactt taggccctct agtagcatat cactcaaagc ctgggaggga accttggccc
     4321 accgctgtgt ggtgtcaaac gccgggtagt gataacgcca c
//
LOCUS       DBMDYA010000168         4336 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4838_length_4336_cov_1.204959, whole genome shotgun sequence.
ACCESSION   DBMDYA010000168 DBMDYA010000000
VERSION     DBMDYA010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_4336_cov_1.204959"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="ACZPFK_05370"
     CDS             <1..455
                     /locus_tag="ACZPFK_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1405773.1"
                     /translation="PKFEGNVKLFEEIAKACKAKGIQLLYHNHDFEFERQEDGRYLLD
                     RILAADDELLLEPDTFWAFFAGVDPAALLEQHRRRVRFIHIKDYLDLEQKPFPAFTAI
                     GTGRMDNAPVLSKAKEIGAEWIVVEQDNSPIDELESARLSLQAMRAWF"
     gene            512..982
                     /locus_tag="ACZPFK_05375"
     CDS             512..982
                     /locus_tag="ACZPFK_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025050.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1405774.1"
                     /translation="MDMKNHLDLDGFIQIAILVEDIEKAAREWAELLGLPVPEIIEQP
                     RPEGPIPGLTYRGGPASYGLRLAVFHAPAGFIIELHQCCDENDSTFHEFVQKHGYGVH
                     HLGFRVGDRRDAVVAELQQRGYELRTVGVSDDNSWTIVDSEGRLGVNLNIKPKL"
     gene            complement(1467..2864)
                     /locus_tag="ACZPFK_05380"
     CDS             complement(1467..2864)
                     /locus_tag="ACZPFK_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405775.1"
                     /translation="MNNDLTVGKPGTVLWRFCLPLFGSILFQQLYNIADSLVAGNFVG
                     ESALAAVGNSYEITLIFIAFAFGCNIGCSVVVSQLFGARDYRGLKTAVSTAWIASGAL
                     CAVLMAAGFAFCGPLLALIRTPADIFADSALYLRIYIAGLFFLMFYNIATGIFSALGD
                     SRTPFLFLAASSTANIFVDVLFVTAFHMGVAGVAWATFLCQGVSCALAMLAVRRRLAG
                     LPAEKAPRFSWAMLRRIGVIAVPSILQQSFISVGNIVIQAVVNGFGSAVIAGYSAAVK
                     LNNMVITSFTTLGNGISNYTAQNIGAGKPRRVRQGFAAGLRMVWLLCLPIIALYLAWG
                     GGLVSFFMEEGSRQAIDTGARFLSILAPFYLVVSAKLVSDGVLRGANAMLPFMAATFT
                     DLVLRVVLAVVFAGLFGVAGIWFAWPVGWAVATALSVFFYRRGAWRRAARDETALAVE
                     KAEAALADGAAEREE"
     gene            3039..3938
                     /locus_tag="ACZPFK_05385"
     CDS             3039..3938
                     /locus_tag="ACZPFK_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1405776.1"
                     /translation="MLVSYEYYRFFYEVARCGSFTRAANALHSSQPNVTRTVRRLEAA
                     LGCRLFERSNRGVQLTPEGERLCARVAVACEQLEAGEADLLSAARLQGGTVFLGATET
                     ALRGFLIEKLAGFHRLHPAVRLKIKSCTTPQALAALRRGEIELGVVTTPAETAAPLKR
                     IELRRFRDILIAAANLPPPQHPLTLAELQRQPLILMGKGTGTHELYSALYQRQGLSLQ
                     ADIEVETSDLLLPMVEQGLGVGFLPDLFARQALAQGRVRELPSAFSLPARSICAVYDG
                     GRSAGAAAAALLRFLRGDGPEGA"
BASE COUNT          783 a         1470 c         1429 g          654 t
ORIGIN      
        1 cccccaagtt cgagggcaac gtcaagctgt ttgaggagat tgccaaggcc tgcaaggcca
       61 agggcatcca gctgctgtac cacaaccacg actttgagtt tgagcggcaa gaggacggcc
      121 gctacctgct ggaccgcatc ctggcagccg acgacgagtt gctgctggag ccggacacct
      181 tttgggcatt cttcgccggg gtggacccgg cggccctgct ggagcagcac cgccggcgcg
      241 tgcgcttcat ccacatcaag gactatctgg acctggagca aaagcctttt ccggccttta
      301 cggccatcgg caccggcagg atggacaacg cccccgtgct gagcaaggcg aaagagatcg
      361 gcgccgaatg gatcgtggtg gagcaggaca acagccccat cgacgagctg gagagcgcgc
      421 ggctcagcct gcaggccatg cgggcctggt tctgacaggg cggggcaaaa cgctttgcaa
      481 acgcagtgaa aagagaaagg ggtgcaaggc catggacatg aaaaaccatc tggacctgga
      541 cggctttatc cagatcgcca ttttggtgga ggacatcgag aaagccgccc gggagtgggc
      601 cgagcttttg ggcctgcccg tgcccgagat catcgagcag ccccgcccgg aggggccgat
      661 ccccggcctc acctaccggg gcgggcccgc cagctatggg ctgcggctgg ccgtctttca
      721 cgcgcccgcg gggttcatca tcgagctgca ccagtgctgc gacgagaacg acagcacctt
      781 tcacgagttc gtgcaaaagc acggctacgg cgtgcaccac ctgggcttcc gggtggggga
      841 ccggcgggac gccgtggtcg cggagctgca gcagcgcggc tatgagctgc gcacggtggg
      901 cgtctcggac gacaacagct ggaccattgt ggacagcgag gggcgtctcg gcgtcaacct
      961 caacatcaag cccaagctct gagaaaggcc ctgcccgggc ttttgggcaa gcggccgcgg
     1021 gcaaagcaaa aaagcaagcg caaacagaaa aaggaggggc gccgtcgcgt tgacggcgct
     1081 cctccttttt agggcccggc ctccccgcgc cgcgggcgcg ggccggggcg tttcgcgcgc
     1141 tctttcgccc tgcggggcgc gccgtgcctt tccgctttgc cacaaatccg cggccgccct
     1201 ccgccgcgcg gggcgcgtgc cgggcgcgct taccgccgtc ggcggcgtcg ggagagccgc
     1261 ccaccggccc acggcaaaga gggcggcagg ccgctttttt cgctttccca ccgtcttttc
     1321 cccagccgct ccgcccggcc gggcgcgtgc cgggcgtgga ggacaattta atgattatgt
     1381 ccggaaatcg gtgttctcct ccgcgcgggc gcgcgcctct ttcagtggga accgttttct
     1441 gcaggaggcg cttttgcgcc ccggggctat tcctcccgtt ccgccgcccc gtccgcgaga
     1501 gcggcctccg ccttttccac cgccagcgcc gtctcgtccc ttgccgccct gcgccaggcg
     1561 ccgcggcggt agaagaacac cgacagcgcc gtggccaccg cccagcccac cggccaggca
     1621 aaccagatgc cggcgacgcc gaacaggccc gcgaagacca ccgccagcac caccctcagg
     1681 acgagatcgg tgaacgtcgc cgccataaag ggcagcatgg cgttggcgcc gcgcagcacg
     1741 ccgtccgaca ccagcttggc cgagaccacc agataaaacg gggccaaaat ggacaaaaag
     1801 cgcgcgccgg tgtcgatggc ctgccgcgac ccctcctcca tgaaaaagga gacaaggccc
     1861 ccgccccagg ccagatacag cgcgatgatg ggcaggcaga gcagccacac catccgcagc
     1921 cccgccgcga agccctgccg cacccgccgc ggcttgcccg cgccgatgtt ctgcgccgta
     1981 tagttggaga tgccgttgcc cagggtggtg aacgaggtga tgaccatgtt gttcagcttc
     2041 accgccgccg agtagcccgc gatcaccgcc gagccgaagc cgttgaccac cgcctgtatg
     2101 acaatgttgc ccaccgagat aaagctctgc tgcaaaatgc tgggcaccgc gatgacgccg
     2161 atgcgccgca gcatggccca ggagaagcgc ggcgcttttt cggccggcag gccggccagg
     2221 cggcggcgca ccgccagcat ggccagcgcg cagctcaccc cctggcagag aaaggtggcc
     2281 caggccaccc ccgccacccc catgtgaaac gcggtgacga acagcacatc cacaaagatg
     2341 ttcgccgtcg aggacgccgc cagaaagaga aacggcgtgc gggaatcgcc cagcgccgaa
     2401 aagatgccgg tggcgatatt gtaaaacatc aaaaagaaca gcccggcgat gtagatgcgc
     2461 agatagagcg cggagtcggc aaagatatcc gccggggtgc ggatcagggc cagcagcggc
     2521 ccgcagaaag caaagcccgc cgccatcagc accgcgcaca gcgcgccgct ggcgatccag
     2581 gcggtggaga ccgcggtctt cagcccccga tagtcccgcg cgccgaacag ctgtgagacc
     2641 accaccgagc agccgatgtt gcagccgaag gcaaaggcga taaaaatcag cgtgatttca
     2701 taggagttgc ccaccgccgc cagggccgac tcgcccacaa aattgcccgc caccaggctg
     2761 tcggcgatgt tatagagctg ttgaaaaagg atgctgccga acagcggcag gcaaaacctc
     2821 cacagcacgg tcccgggctt gcccaccgtc agatcgttgt tcattttttt gctcccctcc
     2881 tgttctgccg cccgcggcgg ggacggggcg aaacagagcc gcatctcccc gtcccgcgca
     2941 cgcagctttt tcattatagt cttgtggagc atcgctgtaa aagatataat ttatatggca
     3001 gtaatatcaa attgatatgg caaagggagg cgcgggccgt gctggtcagc tatgaatatt
     3061 accgtttctt ttatgaggtg gcccggtgcg gcagcttcac ccgggcggcc aacgccctgc
     3121 acagcagcca gcccaacgtc acccgcaccg tgcggcggct ggaggcggcg ctgggctgcc
     3181 gcctgttcga gcgctccaac cggggggtgc agttgacccc tgagggcgag cggctctgcg
     3241 cgcgggtggc ggtggcctgt gaacagctgg aggccggcga ggcggatctg ctgagcgcgg
     3301 cccgcttgca ggggggcacg gtttttctcg gggccaccga gacggccctg cgcggctttt
     3361 tgatcgaaaa gctggccggc tttcaccggc tgcacccggc cgtgcgcctc aagatcaaaa
     3421 gctgcaccac gccgcaggcc ctggccgccc tgcgccgcgg ggagatcgag ctgggggtgg
     3481 tgaccacccc cgccgagacg gccgccccgc tgaagaggat cgagctgcgg cgctttcgcg
     3541 acatcctcat cgccgccgcc aacctcccgc cgccgcagca tcccctgacg ctggcggagc
     3601 tgcagcgcca accgctcatc ctgatgggca agggcaccgg cacccacgag ctttacagcg
     3661 ccctctatca gcggcagggc ctcagcttgc aggcggatat cgaggtggag accagcgacc
     3721 tccttttgcc catggtggag cagggtctgg gggtgggctt tctgcccgac ctgtttgcgc
     3781 ggcaggccct ggcgcagggg cgggtgcggg agctgccgtc cgctttttcg ctgcccgccc
     3841 gctcgatctg cgcggtctat gacggcggcc gctccgccgg ggcggcggca gcggccctgc
     3901 tgcgctttct gcgcggcgac ggcccggagg gggcgtgacc cgccccctcc ggcgccgcac
     3961 ggcttggggc gcgggggcgg cccctctaaa acggaagccc gcaccacgcc gcagcgggcg
     4021 ggacggaaag gaaagacgcg cgccgcagcg gcgaggcgga aaggaaagac gcgcgtcgcc
     4081 cgccccgctt tggcaggagg agccgcgcgg ggggcgcggg cggggcgcgg agccggagcg
     4141 ccgcatttcc attccgtcac aagccttttt tcatcttcag cacccgcttt ccccggaacc
     4201 acgcgccaga cctgtggttt tcggggcgca aaaaaacagg gccgccatga ggacggtcct
     4261 gcttctcttt ttttctcttc gcgccaaacc ggcggcgccg cggctcaggc cgtgcgcccc
     4321 acctcgtcga tggtca
//
LOCUS       DBMDYA010000169         4303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4875_length_4303_cov_1.672061, whole genome shotgun sequence.
ACCESSION   DBMDYA010000169 DBMDYA010000000
VERSION     DBMDYA010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_4303_cov_1.672061"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="ACZPFK_05390"
     CDS             complement(<1..628)
                     /locus_tag="ACZPFK_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1405777.1"
                     /translation="MKQTFSLLLALMLTASLAACGPARPTSLSHSGPAASPIPALSPA
                     PTKTPSVGSASAQEGKVLVAYFSATGATARVAAEIAAYLNADLFEITPETPYTEADLD
                     YNGDCRANDEQQNDLVRPALAAGCTVEDWDSYEVVFLGHPIWWGIPPKIMRTFAEQYD
                     WSGKTIVPFCTSGGSEYSAEGLPSDARPPAEKRTPPAKMPAKNTEPSCP"
     gene            complement(727..1593)
                     /locus_tag="ACZPFK_05395"
     CDS             complement(727..1593)
                     /locus_tag="ACZPFK_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3737 family protein"
                     /protein_id="HIR1405778.1"
                     /translation="MREQILEHQTFDRERALYGSRGLTVRHCAFDGPADGESAFKESE
                     DITAEDCFFNLRYPFWHVHGLAIRRSQMTELCRAALWYSDHIEIRDSGLHGIKALREC
                     DHASFKNCDIVSPEFGWSARDVKMEDCTAESEYFFLRGERLDFRGVSFKGKYSFQYVK
                     GGVFDRCVFDTKDAFWHSENVTVTDSVVKGEYLAWYSQGLTLIRCKIIGTQPFCYCKG
                     LRLIDCEMVGADLAFEKSQVEATVTTPILSVKNPRAGTITAPAVGELILDDPEARGEV
                     LLCRSTPAEKGA"
     gene            complement(1705..2247)
                     /locus_tag="ACZPFK_05400"
     CDS             complement(1705..2247)
                     /locus_tag="ACZPFK_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1405779.1"
                     /translation="MDKKVLIISTSLRRESNSELLAQQFARGAKDAGHQVETISLREK
                     TIQFCTGCLACQSTRRCAIRDDANAIVEKMREAEVICFATPVYYYEMSGQMKTLLDRA
                     NALFSADYAFRDIYLLTASAEAEDSAADGTVNGLNGWISCYEKCRLAGVVRGGGAGEP
                     GEMEKLTDKLAEAYRMGSAV"
     gene            complement(2253..2489)
                     /locus_tag="ACZPFK_05405"
     CDS             complement(2253..2489)
                     /locus_tag="ACZPFK_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405780.1"
                     /translation="MGTMEDREIGGCLSACGLGAEQRERYLALARGGQTKEQMRLLWR
                     RRKALMDDLHLVQKQVDRIDFLLRSLERADEIKE"
     gene            2616..3506
                     /locus_tag="ACZPFK_05410"
     CDS             2616..3506
                     /locus_tag="ACZPFK_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1405781.1"
                     /translation="MIETRLLHYFLAVAREQNITRAAEALHVTQSTLSKQMMDLEQQL
                     GKQLLIRGNRSVTLTEEGAFLRKQAQEIMELVDKTESVFHAGDEIVGGDIYLGCAETP
                     AMEEITKVFQSIHADYPGIRFNIFSGDAASVVEKLDKGLLDVGILQEPAIYERFEYTK
                     LPYKDVFGLLMPRDCRLAAKQAVTLDDLEDLPLIFSRQTHHGASRTEWFGARYDKFRI
                     VATYNLLYNATHLVEQGVGYAFCLDGLADTKTRNLTFRPFDPPLEVDIVAVTKKYQPL
                     SPAAKVFIEQVTDLSHKERS"
     gene            3503..3940
                     /locus_tag="ACZPFK_05415"
     CDS             3503..3940
                     /locus_tag="ACZPFK_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405782.1"
                     /translation="MTAFCHGGQARDGSSAQFKRQSCAVPHTPAFSSYYALKLRLSCY
                     GVQNLFEGGSREPGVYLRPYGYVSHRGAVDGSAKSSTQKANFIFFFPLQKLQNEPSLC
                     FLSLRYQKQLILTPFLAAAAVIEMDGLQNNVRVFFILEWVTGL"
     gene            complement(4157..4231)
                     /locus_tag="ACZPFK_05420"
     tRNA            complement(4157..4231)
                     /locus_tag="ACZPFK_05420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4196..4198),aa:Gly,seq:tcc)
BASE COUNT          824 a         1307 c         1251 g          921 t
ORIGIN      
        1 tcgggcagga gggctcggtg ttcttcgcgg gcatcttcgc cgggggcgtc ctcttctcag
       61 ctggcggcct ggcgtcggac ggcagcccct cggcgctgta ttcgctgccc ccgctggtgc
      121 aaaaaggaac aatggtcttg ccgctccagt cgtactgctc cgcgaaagtg cgcatgatct
      181 tcggcgggat gccccaccag atcgggtggc ccaaaaagac cacctcatag ctgtcccagt
      241 cctccacggt acaaccggcg gcgagagcgg gccggaccaa atcgttttgc tgctcgtcgt
      301 tggcccgaca gtcgccgtta tagtcgaggt cggcctccgt atagggcgtt tctggtgtga
      361 tctcaaaaag atcggcattc agatatgccg cgatttccgc agctaccctt gcggtggcgc
      421 ctgtggcgga aaaataggcc accagaacct tgccctcctg cgcagacgcc gagccgacgg
      481 agggtgtctt tgtgggcgcg gggctgaggg cggggatagg actggcggcg gggccggagt
      541 gggagagcga cgtgggccgg gcggggccgc aggctgccag agacgccgtc agcatcagcg
      601 caagcaaaag actgaatgtt tgtttcatgc cgtcgcctct ctttttgccg tgaaagtgat
      661 cgtcccgcgc agccgaaccg cccgaacacc ctttgtcatg gtgcggcttt gttgtcggta
      721 gcatcctcag gccccctttt ccgccggggt tgaccggcat aaaaggacct cgccgcgggc
      781 ctccgggtcg tccaggatca gctccccgac cgcgggggcg gtgatggttc ccgcccgggg
      841 gttctttaca ctcaggatgg gggtggtgac agtggcctcc acctgggatt tttcaaaggc
      901 gaggtcggcg cccaccatct cgcagtcgat gagcctgaga cccttgcagt agcagaacgg
      961 ctgggtgccg atgatcttgc agcggatcag ggtcagaccc tgggaatacc aggccaggta
     1021 ctcccctttc accacgctgt cggtgacggt gacgttttcg ctgtgccaaa aagcgtcctt
     1081 ggtgtcaaag acacagcggt caaagacgcc gcccttgaca tactgaaaag aatatttgcc
     1141 cttaaagctc acgcccctaa aatcgagccg ttcgccgcgc agaaaaaaat attcgctctc
     1201 ggcggtgcag tcctccatct tcacgtccct ggcggaccag ccgaattccg gcgagacgat
     1261 gtcgcagttt ttgaaagagg cgtgatcgca ctcccgcagc gccttgatgc cgtgaagccc
     1321 gctatctctg atctcgatgt gatcggagta ccacagagcg gcccggcaca gctctgtcat
     1381 ctgcgagcgg cggatggcca gcccgtggac gtgccagaaa ggatagcgca ggttgaaaaa
     1441 gcaatcctcc gccgtgatgt cctcgctctc cttgaaagcg ctctccccgt ccgcggggcc
     1501 gtcgaaagcg cagtggcgca cggtgaggcc gcggctgccg tagagcgccc gctcccggtc
     1561 aaaggtctga tgttccaata tctgttctct cattgcaatt ctccttgtgc cgtgcctttg
     1621 agcttcaact gcgaggggga gacggaatgc tccgcgcgcc cggcccgtcc ggcggctgcc
     1681 ccgccttcgc tgtgcccttt gcctctacac ggcgctgccc attctgtagg cctccgccag
     1741 cttgtcggtc agcttttcca tctcgcccgg ctcgccggcc ccgccgcccc gcaccacgcc
     1801 cgcgagccgg catttttcat agcaagaaat ccagccgttc aatccgttta cggtgccgtc
     1861 cgcggcgctg tcctccgcct cggccgaggc ggtcagcaga tagatgtccc gaaaagcgta
     1921 gtcggcgctg aagagggcgt tggcacgatc cagcagcgtc ttcatctggc cggacatctc
     1981 gtaatagtag accggcgtgg caaagcagat cacctccgcc tcccgcatct tctccacgat
     2041 ggcgttggcg tcgtcccgta tcgcgcaccg tcgtgtgctc tggcaggcaa gacagcccgt
     2101 gcagaattgg atcgtctttt cacgcaggga aatggtttcc acctggtgcc ccgcgtcttt
     2161 cgcaccccgg gcgaactgct gggccagcag ctcggaattg ctttcccggc gcaggctggt
     2221 ggagatgata agtacctttt tatccattgt ggtcactcct ttatttcgtc ggcccgctcg
     2281 agagagcgga gcaaaaagtc gatgcggtcc acctgttttt gaaccaggtg cagatcgtcc
     2341 atcaaggcct tgcgccgccg ccaaagcagc cgcatctgct cttttgtctg cccgcccctc
     2401 gcaagggcca gataccgttc ccgctgctcc gccccaagcc cgcaggcgct cagacagccg
     2461 ccgatctccc ggtcctccat ggtccccata ggctccctct cttccgtttc tattctacat
     2521 cctcttttta aaattacaaa cagttgtttt tcacgtctag aaatcgttga caggaatagt
     2581 aatgggagtt aaactgaaaa aaaggaggtg agggcatgat cgagacaagg ctgctgcact
     2641 attttctcgc cgtcgccagg gaacagaaca tcaccagagc ggcggaggcc ctgcacgtca
     2701 cccaatccac gctctcaaag cagatgatgg acctggagca gcagctggga aagcagcttc
     2761 tgatccgcgg gaaccgaagc gtcaccctca ccgaggaggg ggcctttctg cgcaagcagg
     2821 cccaggagat catggaactg gtggacaaga cggaatccgt ctttcacgcc ggggatgaga
     2881 ttgtcggcgg ggacatttat ctgggctgtg cggagacccc cgccatggag gagatcacaa
     2941 aggtctttca gtccatccac gccgattacc cgggcatccg cttcaacatc ttcagcggcg
     3001 acgccgccag cgttgtggaa aagctggaca aagggctgct ggacgtgggc attttgcagg
     3061 agccggccat ttatgagcgg tttgaataca caaaactgcc ctacaaagac gtgttcggcc
     3121 ttttgatgcc gcgggactgc cggctggccg ccaaacaggc ggtgacactg gacgacctgg
     3181 aggacctgcc gctgatcttc tcccggcaga cccatcacgg cgcctcccgg acggagtggt
     3241 tcggcgcgcg ctatgacaaa ttccgcatcg tcgccaccta caatcttctc tacaacgcca
     3301 cccatctggt ggagcagggc gtcggctacg ctttttgtct ggacggcctg gcggatacga
     3361 aaacccgcaa tctgaccttt cgccctttcg acccgccgct ggaggtggac attgtggcgg
     3421 tgacgaaaaa gtatcaaccg ctctcgccgg cggcaaaggt gtttatagaa caggtgacgg
     3481 acctttctca caaggagcgc tcttgaccgc tttttgccac ggcggacagg cccgggacgg
     3541 aagctccgcg caattcaaaa ggcagagctg cgccgtgcct cacacccctg ctttttcttc
     3601 gtactatgcg ctcaagctga ggctgtcgtg ttatggcgtt cagaaccttt tcgagggcgg
     3661 ttcaagggaa ccaggcgtct atttgcgtcc ctatggatac gtttcacaca gaggagcagt
     3721 tgacggttct gcaaaaagca gcacgcaaaa agccaatttc attttctttt tcccgctgca
     3781 aaagctccaa aacgagccgt cactttgttt tttgtcgctt cgctatcaaa agcaacttat
     3841 cttgacgccc tttctcgccg ctgccgcggt aatcgaaatg gatggcctcc aaaataacgt
     3901 acgggtattt ttcattttgg agtgggttac tgggttataa agtgggttat ttcaaaaata
     3961 aaccccggtg aaatgctgat ttcaccgggg tttgggagag aacaacggaa aggagttcag
     4021 cgcgctgcgc ggcagtccgc cggactgccg ctggcccgct caagcttcgc cttacggctc
     4081 cctttcgcgg gagcgctttg ttcgactccc tccacctgaa ttaaaaaagc gccccctgcg
     4141 gggacgcttt ttcttttgga gcaggtgaag ggagtcgaac cctcgtccgc agcttggaag
     4201 gctgctgtac tagccgttgt acgacacctg cgtctctgtc ccccgcgtgc gggggacagt
     4261 gttaattata ccgttttcgg ggctgcgctg tcaagcggca tgc
//
LOCUS       DBMDYA010000170         4301 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4877_length_4301_cov_1.666905, whole genome shotgun sequence.
ACCESSION   DBMDYA010000170 DBMDYA010000000
VERSION     DBMDYA010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4877_length_4301_cov_1.666905"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="ACZPFK_05425"
     CDS             <1..361
                     /locus_tag="ACZPFK_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HIR1405783.1"
                     /translation="PPMRLYGLHPDRLYEVTVRREVINIKTFGSLINQILPFRVNADG
                     LLMHTASGVYMLPCEEERYTAWGDLLMRAGLRFKQSFTGTGYNDDVRLMPDFSGRLYA
                     LKAGAGTLPGEKQSKNE"
     gene            653..1591
                     /locus_tag="ACZPFK_05430"
     CDS             653..1591
                     /locus_tag="ACZPFK_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1405784.1"
                     /translation="MIDIYMTNPANGALAEQEKILPGCWVHLCDPTPEEIGRVSSCLS
                     IDRDFLAAALDEEERSRLEVEDGNTLVLVDTPTVETQGPNFVYNTLPFGIILTEENIV
                     TVCLKEVALPRAFTEGLVKGLNTRKKNRMTLQFLYRNATLFLFYLRQVDKNSTRVENE
                     LHISMKNKELIQMLGLEKSLVYFSTALKSNEIVLEKMLRLDFVKDYPEDTELLEDVII
                     ENKQAIEMSNIYRDILSGTMDAFASVISNNLNIVMKLLAAVTIVLTIPSIIFGLWGAN
                     VPVPFESNPLGFVYIVLIAAVFTIAAVAVMIRKKWL"
     gene            complement(1672..1899)
                     /locus_tag="ACZPFK_05435"
     CDS             complement(1672..1899)
                     /locus_tag="ACZPFK_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1405785.1"
                     /translation="MEKVVELLQEIKDGVDYNSCTTLIDDAIFDSFDIIQTINALNDE
                     FDLSIPATEIIPENFNSAAALWAMVQRLLDE"
     gene            complement(1900..3084)
                     /locus_tag="ACZPFK_05440"
     CDS             complement(1900..3084)
                     /locus_tag="ACZPFK_05440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1405786.1"
                     /translation="MRDTQWREIAERYGTPCYIFDADVLTRRIEKIQQVLGPSVSLCY
                     SIKANPFLVRSVQPLIRYLEVCSPGEFDICKALSVPMEKVLLSGVCKAPEDIADAVAC
                     SAALYTAESPRQARLLEKAAGRAGKRLPVLLRITAGSQFGMDEADFFSLLSSSDFPHL
                     EFIGIHYFSGTQHTKSAKLLAEIDYLDQLLGRAAIQCGWKAQKVEYGPGLAVPYFEGD
                     DFSFPMAPLEAIARPLRALAAKVDLTVEMGRFFAAECGYYLTRIVDLKTSGKTQYAFV
                     DGGINHVNYYGQMMGMKVPVIHTLSEGPAVPPSHDYTLCGSLCTLADVLVRKVSLPRL
                     EIGDILVLCNIGAYSVTEGLYLFLSRRMPRVILYDSAHGARLVRENIDTARLNMERGS
                     VF"
     gene            complement(3089..>4301)
                     /locus_tag="ACZPFK_05445"
     CDS             complement(3089..>4301)
                     /locus_tag="ACZPFK_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1405787.1"
                     /translation="LCTDEQHREQAQALPFRGSLVVLEDIRNTFPDHSALLRIRELAA
                     DTDPLYAIFTSGSTGVPKGVAVCHRSVLDFIPQFTALMGIEEEDVLGNQAPFDFDVSV
                     KDIYSALYSGATVHIIPRAYFSFPTKLMDYLCERRVTTLVWAVSAMCFVSTMRGFDYR
                     VPADVRKVIFSGEVMPVKHLNIWRTFLPRAKYFNVYGPTEITCNCTYYAIERSFSEEE
                     ILPIGRPFPNERVFLLDEEDHLVTAPGALGELCVSGTALALGYYNDPERTAQVFVQNP
                     LHHRYPETIYRTGDLARYDQDGNLVYVSRKDFQIKHMGHRIELGEIDAVLGRQRGVER
                     ACCLYYPEKQRLLAFYLGSAEKRELFAALRQILPGFMLPNALIPCTGWPLTKNGKTDR
                     ARLLADYLERG"
BASE COUNT          946 a         1258 c         1261 g          836 t
ORIGIN      
        1 gccgcccatg cgcctgtacg gcctgcaccc ggaccgcctg tatgaggtga cggtgcgccg
       61 cgaggtcatc aacatcaaga cgttcggcag cctcatcaac cagatactgc ccttccgggt
      121 gaacgcggac ggcctgctga tgcacaccgc cagcggggtt tatatgctgc cctgcgagga
      181 agagcgctac accgcctggg gcgacctgct gatgcgggcg gggctgcgct tcaagcagtc
      241 gttcaccggc accggctaca acgacgacgt gcgcctgatg cccgattttt ccggccgcct
      301 ctacgcgctc aaagcgggcg cgggcacgct gccgggagaa aagcagagca aaaacgagta
      361 aagggcaagc gggcctggca aaaaccgtaa aattggggca gatgcggccg tttttcttga
      421 cagatcgccc taaaatggca taaactaaga aaggactggg agacggcgca gcgccttggc
      481 ctgcgccgtt tttgcggcct gatcccggga gaaaaaggag tgtgaaccgc tcgtggaaag
      541 cgaacccaac cctcgaagac gcaatcataa cgccggcccc gccggcgtcc ccgcgggcgg
      601 cgtgcgcccc tgaggacgcc tgccccgggc cgggaaagca ggtgaccaga ctatgatcga
      661 catctatatg accaaccccg ccaacggcgc cctggccgag caggaaaaga tcctgcccgg
      721 ctgctgggtg cacctctgcg acccgacgcc ggaggagatc ggccgggtgt ccagctgcct
      781 gtccatcgac cgcgattttc tcgcggccgc cctggacgag gaggagcgca gccgcctgga
      841 ggtggaggac ggcaacacgc tggttctggt ggacaccccc accgtggaga cccagggccc
      901 gaacttcgtc tacaataccc tgccgttcgg catcatcctc accgaggaga atatcgtcac
      961 cgtctgcctg aaagaggtgg cgctgccccg cgcgtttacc gaggggctgg tcaaggggct
     1021 caacacccgc aaaaaaaacc gcatgaccct gcagttcctc taccgcaacg ccaccctgtt
     1081 tttgttctac ctgcgccagg tggacaaaaa cagtacccgg gtggaaaatg agctgcacat
     1141 cagcatgaag aacaaggagc tcatccagat gctggggctg gaaaaaagcc tggtctattt
     1201 ttccaccgcg ctcaagagca acgagatcgt gctggagaag atgctgcgcc tggattttgt
     1261 caaggattac ccagaggaca ccgagctgct ggaggacgtc atcattgaga acaagcaggc
     1321 catcgagatg agcaatatct accgggacat cctgagcggc accatggacg ctttcgcctc
     1381 ggtcatctcc aacaacctga acatcgtcat gaagctgctg gccgccgtga ccattgtgct
     1441 caccatcccc tccatcattt tcggcctgtg gggcgccaac gtgccggtac cctttgagag
     1501 caacccgctg ggctttgtct acatcgtgct catcgccgcg gttttcacca tcgcagccgt
     1561 ggcggtcatg atccgcaaaa agtggctgta ggggcggggg gcgaaaaaag agaaaaaggg
     1621 aggagacggc gaaagccgtc tcctcccttt tttattgatt caggcaagcc ctcattcatc
     1681 cagcaagcgc tgtaccatcg cccacagggc ggcggcactg ttgaaatttt cggggatgat
     1741 ctcggtggcg gggatcgaga gatcaaactc gtcgttgagc gcgttgatgg tctgaatgat
     1801 atcaaaagaa tcaaagatcg cgtcgtcgat cagggtggtg caggaattat aatccacccc
     1861 atctttgatc tcttgcagaa gttcgactac tttttccatt taaaaaacag accctctttc
     1921 catgttcaat cgtgcggtgt cgatgttttc gcgcaccagc cgtgcgccgt gcgccgagtc
     1981 gtataagatc acccggggca tcctgcggct caaaaaaaga tagaggccct cggtgacgga
     2041 ataggcgccg atattgcaca gcaccagtat atcaccaatt tcaaggcgtg gcaacgacac
     2101 tttcctcacc agcacatccg ccaaagtgca caggctgccg cacagggtat aatcgtgtga
     2161 tgggggcaca gcgggcccct ctgatagggt atggatgacc ggaactttca tccccatcat
     2221 ctgcccatag taattcacat ggttgatgcc gccgtccaca aaagcgtact gtgtttttcc
     2281 gcttgtcttc aaatcgacga tgcgggtgag atagtagccg cattccgccg caaaaaaacg
     2341 ccccatttcc accgtgaggt ccaccttggc cgccagcgcc cgcagcggcc gggcaatggc
     2401 ctcgaggggg gccatgggaa aagaaaagtc atccccctca aaatagggca cggccaagcc
     2461 gggcccatat tccacttttt gcgctttcca gccgcactgg atggccgccc ggcccagaag
     2521 ctggtccaga tagtcgatct ccgccagcag ctttgccgac ttggtgtgct gggtgccgct
     2581 gaaatagtgg atccctataa actccagatg aggaaagtcg gaagaggaga gcaatgaaaa
     2641 gaaatcggcc tcatccatcc cgaattggct gcccgccgtg atccgcagca gcaccggcag
     2701 ccttttgccg gctcggccgg cggccttttc cagcaggcgg gcctgccgag ggctttccgc
     2761 agtgtagagc gccgcgctgc aggcgaccgc atcggcaata tcctcgggtg ctttgcacac
     2821 gccggacaac aacacttttt ccatgggcac actcagcgct ttgcagatat cgaattctcc
     2881 ggggctgcac acctcaagat agcggatcaa aggctggacg ctgcgcacca aaaaagggtt
     2941 ggccttgatg gaatagcaaa ggcttaccga gggccccagc acctgctgga ttttttcaat
     3001 gcgccgggtc agcacatccg cgtcaaagat gtagcagggt gtcccatagc gctctgcaat
     3061 ctcgcgccac tgtgtatcgc gcatcccctc agcccctttc cagataatct gcgagcagcc
     3121 gggcccggtc ggtctttccg tttttggtca gcggccagcc ggtgcagggg atcagcgcgt
     3181 ttggcagcat aaagcccggc agtatctgcc gcagcgctgc aaatagctcc cttttctccg
     3241 cgctgcccag gtaaaaggcc agcagacgct gtttttccgg atagtaaagg cagcaggccc
     3301 gctccactcc acgctggcgt cccagcacgg cgtcgatctc ccccagttcg atgcggtggc
     3361 ccatgtgctt gatttgaaaa tctttgcggg aaacatagac gaggttgccg tcttggtcat
     3421 agcgggcaag atcgccggtg cggtagatgg tctcgggata gcggtgatgc agcggatttt
     3481 gcacaaagac ctgtgcggtg cgttcgggat cgttataata tcccagcgcc agggcggtgc
     3541 ccgacacgca caactcgccc agggcgccgg gggcggtgac gagatgatct tcttcatcca
     3601 gcaggaatac acgctcgttg gggaatggcc gaccgatggg gaggatctct tcttcggaaa
     3661 aggaacgctc gattgcgtaa taagtgcaat tacaggtgat ttcggtgggg ccgtaaacgt
     3721 tgaaatattt tgctctgggc agaaaggtgc gccagatgtt gaggtgcttc accggcatta
     3781 cctctccgct gaaaataact ttgcgcacgt ccgcggggac cctgtaatca aatccgcgca
     3841 tggtactgac aaagcacatg gcgctcaccg cccagaccag ggtggtcacc cgccgctcac
     3901 acagatagtc catcagcttt gtgggaaaac tgaaatacgc gcgggggata atgtgtaccg
     3961 tggcgccgct ataaagcgcg ctgtagatat ctttgaccga cacgtcaaaa tcaaaaggcg
     4021 cttggttgcc cagcacatct tcctcttcga tccccattag tgcggtgaac tgggggatga
     4081 agtccaatac gctgcgatgg cagacagcga cccctttggg caccccggtg ctgccgctgg
     4141 taaaaatggc atagaggggg tcggtgtcgg cggcgagctc tcggatgcgg agcaaggccg
     4201 aatggtcggg gaaagtgttg cggatgtcct ccagcacgac gagagagccg cgaaagggga
     4261 gggcctgtgc ctgttcgcga tgctgctcat ccgtgcacaa g
//
LOCUS       DBMDYA010000171         4295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4884_length_4295_cov_1.726276, whole genome shotgun sequence.
ACCESSION   DBMDYA010000171 DBMDYA010000000
VERSION     DBMDYA010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_4295_cov_1.726276"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="ACZPFK_05450"
     CDS             complement(<1..468)
                     /locus_tag="ACZPFK_05450"
                     /inference="COORDINATES: protein motif:HMM:NF042574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6709 family protein"
                     /protein_id="HIR1405788.1"
                     /translation="MFEKLKRQSLKKSLIFTVILVAAAVALLVSTGFGVFKAIGGPKD
                     LYSLSEDQLNGAYVEVDIYGMYAGYAETTESSSGKPKRTVSREYVIDANDTSYMGLVL
                     RSEDLSAAEDLLQESWDYLNGLTDGTSTLSNGAGKLADGTGDLADGTDKLISGI"
     gene            complement(680..967)
                     /locus_tag="ACZPFK_05455"
     CDS             complement(680..967)
                     /locus_tag="ACZPFK_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405789.1"
                     /translation="MSEILEAAMVICFGLSWPISIRKAYLARTNQGKSLAFLILITTG
                     YVCGIAAKLLSGNITYVFFFYVLNLCMLLTELGLYARNLRLDRQGAAEQAA"
     gene            complement(1036..3288)
                     /locus_tag="ACZPFK_05460"
     CDS             complement(1036..3288)
                     /locus_tag="ACZPFK_05460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1405790.1"
                     /translation="MTSGLTRQEQLLSRLIEEYSLDFSERLTLKTAQDFIYLSDIETY
                     ELYLISTSDEGELPPQWRGYAGRKCYEVLQNRTDPCPFCTNSRLVRDQYYIWQHYNPI
                     AGRNYILKDKLVDYKGRQVRMEVVIDVSTSSRVYKVLQNSIESQNVLAACLEPLVAEN
                     DPGTAFLKLLGHIGRFFGAEWGFIHDFTGDTRLTLWAQDGRAHRALVEQPSPAALADW
                     GARLAPGRQVLMRDGDGEKLSPSPAERRLLEQRGIRSLCITPIFIGARLAGFLVLDNI
                     RRHWPELSVLNTLCSYLSSLIQKEELLAENDRLQYYDPLTGRLNFEGFKRRARQLLEG
                     RGGRGYAVWYGDIKRFKYINDAFGYDAGDRLLRHWAGLLTAEEREGDEVFCRVSADNF
                     VALRRCDGEEDLYPRFERMARELGAFPELARRRFMPELAAGVYLVGDNAEGLSLEEMI
                     NRANMAQKSVKDGRGSRLALYDEALRSRVLREMELDGRLHAALQHREFELYLQPQVPA
                     SPEARRGPLRAEALVRWRDPDGRMIQPGEFIPLFERSGKIAQVDLYMLQEACRFLVKA
                     RQALGRPLCVAVNVSRISMLRPGFVEDYRRVRDAWGVPAGALELEFTESIAVENFEPF
                     REAVTALRQSGFLCAMDDFGTGQSSLNCLQNLPLDVLKLDQAFFLSASDPGRRDTVVA
                     SVLAMARRLSMRTVAEGVESAGEARLLREMGCDYIQGYVYSRPVPAEQYLQQVQEALD
                     GGAAPSPGQG"
     gene            3645..4130
                     /locus_tag="ACZPFK_05465"
     CDS             3645..4130
                     /locus_tag="ACZPFK_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405791.1"
                     /translation="MDPKIACGHFLLLRNKKQLILMATFLAAMRQPKRMAEKIACGYF
                     LLLRNKKQLILMATFLAAMRQPKRMAEKIACGYFLLLRNKKQLILMVPFLAAMRQPKR
                     MAEKIACGYFLLLRNKKQLISMASFLAAARQPKRMAEKIACGYFLLYPGRVQKSTKTR
                     A"
BASE COUNT          842 a         1433 c         1236 g          784 t
ORIGIN      
        1 gatgccgctg atgagcttgt cggtgccgtc tgccaggtcg ccggtgccgt cggccagctt
       61 gcctgcgccg ttgctgaggg tgctggtgcc gtcggtgagg ccgttcagat agtcccagga
      121 ttcctgcagc aggtcctcgg cggccgacag gtcctcactt ctcagcacca ggcccatata
      181 agacgtgtcg ttcgcgtcga tgacatactc ccggctgacg gtgcgcttcg gcttgccgga
      241 agagctctcg gtggtctcgg catagccggc atacatacca tagatgtcca cctctacata
      301 ggcgccgttg agctgatcct ccgacaggct gtacaggtct ttggggccgc cgatggcctt
      361 gaacacgccg aagccggtgc tcaccaacag cgccaccgcc gccgccacca aaatgacggt
      421 aaagatgagg gattttttga ggctctgcct cttcaatttt tcaaacatgg aattcctcct
      481 tttcgacttc acacacaagg ccattatatc ataccgctcc gccaaagaaa agcgaaaaac
      541 gcgctgccct tgccccgccg cggcgcggcg aaaaaagggt ccggctccgg cgcgccgggg
      601 gcaaaaaaac cgggcgggcg cagcgcgccc gtccggccaa agcaattctg tttttgaaca
      661 gcgccgtgaa cgccgccgct caggcggctt gctccgccgc cccctggcgg tcaaggcgca
      721 ggttgcgcgc atagagcccc agctcggtga gcagcatgca gaggttgagc acatagaaaa
      781 agaacacata ggtgatgttg cccgacagca gctttgccgc gatgccgcag acgtagccgg
      841 tggtgatgag gatcagaaac gccaggctct tgccctggtt ggtgcgggcg agatacgcct
      901 tgcggatcga aatcggccag gagaggccga aacagatgac cattgccgcc tcgagaattt
      961 cagacatttt tgatttcccc ctctttttta tcccgccgct gcgggaagca tccttttcac
     1021 accgcccggc ccctctcagc cctgccctgg cgagggtgcg gccccgccgt ccagggcctc
     1081 ttgcacctgc tgcagatatt gctccgccgg gaccggcctt gaatagacgt acccctgtat
     1141 gtaatcgcag cccatctccc gcaacagccg cgcctccccg gcgctttcga ccccctccgc
     1201 caccgtgcgc atcgagaggc gccgcgccat ggccagcacc gaggcgacca cggtgtcccg
     1261 ccgcccgggg tcgctagccg agagaaagaa agcctgatcc agcttgagca cgtccagagg
     1321 caggttctgc aggcagttca gcgaggactg cccggtgccg aaatcgtcca tggcacacag
     1381 aaagccgctt tgccgcagcg ccgtcaccgc ctcgcggaaa ggctcgaaat tttccaccgc
     1441 gatgctctcg gtaaactcca gctccagcgc gcccgcgggc acgccccagg cgtcccgcac
     1501 ccggcggtaa tcctccacaa agccggggcg cagcatgctg atgcgggaga cgttcaccgc
     1561 cacacagagc ggccgtccca gcgcctgccg cgctttgacc agaaagcggc aggcctcctg
     1621 cagcatgtac aggtccacct gggcgatctt gccgctgcgt tcaaacagcg ggatgaactc
     1681 gcccggctgg atcatccgcc catccgggtc gcgccagcgc accagcgcct cggcgcgcag
     1741 cgggccccgc cgggcctccg gcgacgcggg cacctgcggc tgcagataga gctcaaactc
     1801 gcggtgctgc agagcggcgt gcagcctgcc gtccagctcc atctcgcgca gcaccctgct
     1861 gcgcagcgcc tcgtcgtaga gcgccagacg gctgccgcgc ccgtccttga cgcttttttg
     1921 ggccatgttg gcgcggttga tcatctcctc cagcgagagc ccctccgcgt tgtcccccac
     1981 caggtagacc cctgccgcca gctcgggcat aaagcgcctg cgcgccagct cggggaaagc
     2041 ccccagctcc cgcgccatcc gctcaaagcg cgggtacagg tcctcttcgc cgtcgcagcg
     2101 gcgcagcgcg acaaaattgt ccgccgacac gcggcaaaag acctcgtcgc cctcccgctc
     2161 ctcggccgtc agcagcccgg cccagtggcg cagcagccgg tcgcccgcgt cgtagccgaa
     2221 cgcgtcgttg atgtatttga atcttttgat atccccgtac cagaccgcgt agccgcggcc
     2281 gccgcggccc tccagcagct ggcgcgcccg ccgcttgaac ccctcaaagt tcagccgtcc
     2341 cgtcagcggg tcgtagtact gcaggcggtc gttttccgcc agcagctcct ccttttggat
     2401 caggctggac agatagctgc acagggtgtt gagcaccgac agctccggcc agtggcgccg
     2461 gatgttgtcc agcaccaaaa aaccggccag ccgcgcgccg atgaagatcg gcgtgataca
     2521 gagcgaacgg atgccccgct gctccagcag gcggcgctcg gccggggagg gcgaaagctt
     2581 ctctccatcc ccatcccgca tcagcacctg gcggccgggg gccagccgcg cgccccaatc
     2641 cgccagggcg gcgggggagg gctgctccac cagcgcccgg tgggccctgc cgtcctgcgc
     2701 ccaaagggtg agccgggtgt cgccggtaaa gtcgtggata aacccccact cggcgccaaa
     2761 aaagcggccg atatgcccca gcagcttgag aaacgcggtg ccggggtcat tctccgccac
     2821 cagcggctcc agacaggcgg ccagcacgtt ctggctctcg atgctgtttt gcagcacctt
     2881 gtacacgcgg ctgctggtgg acacgtcgat cacgacctcc atgcgcacct gacggccctt
     2941 gtagtccacc agcttgtcct tgaggatata attgcgcccg gcgatggggt tgtagtgctg
     3001 ccagatatag tactggtcgc gcaccaggcg gctgttggtg cagaaagggc aggggtcggt
     3061 gcggttctgc agcacctcat agcatttgcg cccggcgtat cccctccact ggggcggcag
     3121 ctctccctcg tcggaggtgc tgatgagata gagctcgtag gtctcgatat cgctgagata
     3181 gataaaatcc tgcgccgtct tgagagtgag ccgctcgctg aaatccagtg agtactcctc
     3241 gatcaggcgg gagagcagct gctcctgccg cgtcagtccg ctggtcaacc gctctccccc
     3301 ctcatcggcc ccttcagggc gcgccgtttt tatcgctgat ctattggaaa caagcttccg
     3361 cccgccgtgg tcacggcgat cggaacgggt gactgaaaaa acagccctgt gggctgccgg
     3421 tccacggcct tgaacgccgg ggcgcccgcc cagaaagcag acgaatgcgc gcaaaaagca
     3481 gcggcgcggc tcttatttcc atgccagcgg cccaaggaga aaccggggcc gcaaaacaat
     3541 ctgcagttat ttgtatgccc cggccagaaa actgacccta tgtccataaa aaataattcc
     3601 tcttgacggc cccgcttttc gccgctgcgc ggcaaccgaa accgatggat ccaaaaatag
     3661 cttgcggcca ttttttattg ctccgcaata aaaaacaact gatcctaatg gccacgtttc
     3721 tcgccgctat gcggcaaccg aaacggatgg ccgaaaaaat agcctgcggc tattttttat
     3781 tgctccgcaa taaaaaacaa ctgatcctaa tggctacgtt tctcgccgct atgcggcaac
     3841 cgaaacggat ggccgaaaaa atagcctgcg gctatttttt attgctccgc aataaaaaac
     3901 aacttatctt gatggtcccg tttctcgccg ctatgcggca accgaaacga atggccgaaa
     3961 aaatagcctg cggctatttt ttattgctcc gcaataaaaa acaacttatc tctatggcct
     4021 cgtttctcgc cgctgcgcgg caaccgaaac gaatggccga aaaaatagcc tgcggctatt
     4081 ttttattata cccgggtagg gtgcaaaagt ccaccaaaac gcgcgcctga acggatgtcc
     4141 gatgtgggcc cggcggtcgt ttgccccgtt ttttcccccg ccccctgcgc cgcacaaaaa
     4201 agctgccggc ccaggcgcgt tgaaaggaga aagcattggt ttcaaaccgg aggtcttgac
     4261 gccctgcaaa gacaaaaaag gtccggcaac ccaca
//
LOCUS       DBMDYA010000172         4284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4904_length_4284_cov_1.386326, whole genome shotgun sequence.
ACCESSION   DBMDYA010000172 DBMDYA010000000
VERSION     DBMDYA010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_4284_cov_1.386326"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="ACZPFK_05470"
     CDS             complement(<1..211)
                     /locus_tag="ACZPFK_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1405792.1"
                     /translation="MFHAKNGVLALEKHTMDYISFGSGSETLIMLPGVGDGLRTVKGT
                     ALPFAWMYRAFAKQYTVYVFSRVNSL"
     gene            839..1360
                     /locus_tag="ACZPFK_05475"
     CDS             839..1360
                     /locus_tag="ACZPFK_05475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1405793.1"
                     /translation="MSLNPVRHFDLFGSVSLLLVGIGWAKPVPINARNFDHPRAGMAI
                     SAAAGPAANLVMALLTMILAKVALFAGAGSRVSAVLYVVLLNMCLINISLAIFNLMPF
                     PPFDGSRIYSAFLPQRLYFGVMRYEKYILIAVLALLWLGVLDGPLGFLNHALFSGLDA
                     VTGWVDLLFGLAR"
     gene            1378..2088
                     /locus_tag="ACZPFK_05480"
     CDS             1378..2088
                     /locus_tag="ACZPFK_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1405794.1"
                     /translation="MENLTFRLTDFEGPLDLLLYLISKNKMKIADIEIVSLIDQYLAV
                     VNGPQGATLDEASEFIEMAARLIYMKSVFLLPRSDEQDQLRQELTGMLVEYSACKQVA
                     ATLGRMAAGTYLAVRRPMEVELDATYRIRHDPAQLEQAYCSLLGGARRRERPTQERFE
                     PLVAAPFVSVASKVVYVLRNLVKGRVKYLSSLFRRSASRSENVATFLAVLELLKAGRV
                     TLDEDARLTLRKKRESRG"
     gene            2092..2694
                     /gene="scpB"
                     /locus_tag="ACZPFK_05485"
     CDS             2092..2694
                     /gene="scpB"
                     /locus_tag="ACZPFK_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1405795.1"
                     /translation="MDLKQYRAALEAVLFAVAEPVSTEKLAEILGIEHQVVERLCGHL
                     RDEYAEEEHGIQLLCLEGRWQFSTKGQFGEYVKAALDNRRNVPLSAAALEVLAVIAYN
                     QPVTRSFIEQVRGVDSSGVVQSLAQKGLIEEAGRLDLPGRPIAFRTTDVFLRSFGLSS
                     LAELPPLHGDEMSAEEVAARMDEAALEAEGAERDGAPEEA"
     gene            2694..3392
                     /locus_tag="ACZPFK_05490"
     CDS             2694..3392
                     /locus_tag="ACZPFK_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022615.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HIR1405796.1"
                     /translation="MRILGLVLLCLLAALLLLLLALLFLPAWAILELKYDKLTVRVRV
                     LFLTFTVFPMKGKEPEEPKPQQEAPAGQKKRSPGKKFELTFSRVMRWVADAAGILRMA
                     LAALRFRGIRLTLPVQGEDAADTALFYGKFSAWFYAGVATLQNFLDLRFDHIELIPDF
                     AGENKYRRSFYCKIGATPFIMLVVAFKAFGLLRRDGLLPGSGPGEKKAPPSQEGTAAA
                     PRAGGADIGGSENE"
     gene            3385..3777
                     /gene="ytfJ"
                     /locus_tag="ACZPFK_05495"
     CDS             3385..3777
                     /gene="ytfJ"
                     /locus_tag="ACZPFK_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR1405797.1"
                     /translation="MSEHPLQGLMDVALQRIKEMVDSNTIIGEPITMADGTLILPVSK
                     VSFGFASGGSDFPSKTPKELFGGGSGAGVSISPIAFLVVKDGNVKMLQLASQEANATD
                     RIINALPDVLDKLQDMFKKEKPGADGQG"
     gene            3890..>4284
                     /locus_tag="ACZPFK_05500"
     CDS             3890..>4284
                     /locus_tag="ACZPFK_05500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1405798.1"
                     /translation="MRDTRIQKVLSELGVCSRRAAEEMIRQGRVKLNGRPVQVGDKMD
                     TAKDLISVDGRNIRVPKKQEFVYEMLYKPRGYITTMSDERGRKTVMELVKDVPARIFP
                     IGRLDKDSEGLLLFTNDGEFANLMMHPSHG"
BASE COUNT          820 a         1241 c         1369 g          854 t
ORIGIN      
        1 caagactgtt gactctactg aacacataaa ccgtatattg cttcgcaaaa gctcgataca
       61 tccacgcaaa aggtaacgca gtgcccttta cagttctcaa gccatcgcca acgccgggga
      121 gcataatcag agtttcgctg ccactgccaa atgagatata atccatagtg tgcttttcaa
      181 gtgccagcac accatttttt gcatgaaaca tatgttttgc ctcctcgtaa attccaattt
      241 gccaagtatg ccttttccgc cgaagatgac ctgcgatttt ttattgcttc gcaataaaaa
      301 acaactgatc ttgatggccc cggccaaggg actggccgga tggccaaaaa atagcttcac
      361 ggctattttt tattgctccg caataaaaaa accttatctt catggccctg tttctcgctg
      421 ctgtcgcagc aaccgaaaca gatggccgaa aaaatagccg cgttgcggct attttttaca
      481 tactatcata ccgacttctt ctgttcaagg tcgtttggcg gaatcgacgg agttttgctc
      541 gtccacactt atctacaacg ggacatgcgg gccttttact tgtcaatccc ggccaaaccc
      601 gatataatat aaaggttcta tagtttacag caccgccggt gcggcggcac gcaggttctc
      661 tccgccccgc aaaagagggg cgggactgtg cgggatggaa ggatcttacg atgcgggacg
      721 agctttttga tatcctggtg cgcgcgatcg tgctggtgac ggcgatccct gtccacgagg
      781 cggcccacgc ctttgtggcg gaccggctgg gcgaccccac cgcgcgggcc atgggcgcat
      841 gtccctcaac ccggtgcgcc attttgacct gttcggcagc gtcagcctgc tgctggtcgg
      901 catcggctgg gccaagcccg tgccgatcaa tgcccgcaac ttcgaccacc cgcgcgccgg
      961 gatggcgatc tccgcggctg cgggcccggc ggcgaacctg gtgatggcgc tgctcacaat
     1021 gatcctggca aaggtggcgc tgtttgcggg cgcgggcagc cgcgtgtcgg ccgtgctcta
     1081 tgtggtgctg ctcaacatgt gcctgatcaa catctcgctg gccatcttca acctgatgcc
     1141 gttcccgccc tttgacggca gccgcatcta cagcgcgttt ttgcctcaaa ggctctattt
     1201 cggcgtcatg agatacgaaa aatacatcct catcgcggtg ctggcgctgc tctggcttgg
     1261 ggtgctggac ggcccgctgg ggtttttgaa ccatgcgctg ttcagcggcc tggacgccgt
     1321 caccggctgg gtcgatctgc tgtttggtct ggcgcgctga tatttcgggg gaaatgaatg
     1381 gaaaatctca cctttcggct gactgatttt gaggggccgc tggatctgct gctctatctc
     1441 atctccaaaa acaagatgaa gatcgccgac atcgagatcg tcagcctgat cgaccaatac
     1501 ctcgccgttg tcaacggccc gcagggggcg acgctggatg aggccagcga attcatcgag
     1561 atggccgcgc ggctgatcta tatgaaatcc gtgttcctgc tgccgcgcag cgacgagcag
     1621 gaccagctgc ggcaggagct gaccggcatg ctggtggagt attccgcctg caagcaggtg
     1681 gccgcgacgc tgggccggat ggccgccggt acctatctgg cggtgcgccg gcccatggag
     1741 gtggagctgg acgccaccta ccgcatccgg cacgacccgg cccagctgga gcaggcctat
     1801 tgcagccttt tgggcggcgc gcggcggcgg gagcgcccca cgcaggagcg gtttgagccg
     1861 ctggtggccg cgccctttgt ctcggtggcc agcaaggtcg tgtatgtgct gcgcaacctg
     1921 gtcaaggggc gggtcaaata tctttcgtcc ctgttccgcc gctcggcgag ccgcagcgag
     1981 aacgtggcca cctttctggc cgtgctagag ctgctcaagg cgggcagggt cacgctggac
     2041 gaggacgcgc ggctgacgct gcgcaaaaag cgggagagca gggggtaagc aatggacttg
     2101 aagcagtacc gggccgccct ggaggcggtg ctctttgcgg tggccgagcc ggtctccacc
     2161 gaaaagctgg ctgagatatt gggtatcgag caccaggtgg tggagcgcct gtgcggccat
     2221 ctgcgcgacg aatacgccga ggaggagcac ggcatccagc tgctctgcct ggaggggcgg
     2281 tggcagtttt ccaccaaggg gcagttcgga gaatatgtga aagcggcgct ggataaccgg
     2341 cgcaacgtgc ctctgtcggc ggcggcgctg gaggtgctgg cggtcatcgc ctacaaccag
     2401 ccggtgaccc gcagctttat cgagcaggtg cgcggcgtgg actccagcgg ggtggtgcag
     2461 tcgctggcgc aaaagggcct catcgaggag gcgggcaggc tggacctgcc gggcaggccc
     2521 atcgcctttc gcaccaccga cgtgtttttg cgcagcttcg gcctctcctc cctcgccgag
     2581 ctgccgccgc tgcacgggga cgagatgtct gccgaggagg tggccgcccg catggatgaa
     2641 gcggctctgg aggcggaggg cgccgagcgg gacggcgccc cggaggaggc gtgatgcgca
     2701 tcctcggcct ggtgctgctc tgcctgctgg cggcgctgct gcttttgctg cttgccctgc
     2761 tctttttgcc tgcctgggcg attctggagc tgaaatacga caagctgacc gtgcgggtgc
     2821 gggtgctgtt tttgacgttc accgtctttc cgatgaaagg aaaagagccc gaggagccaa
     2881 agccgcagca ggaggccccg gccgggcaga aaaagcgcag ccccggcaaa aagtttgagc
     2941 tgaccttttc cagggtcatg cgttgggtgg cggatgcggc gggcatcctg aggatggcgc
     3001 tggcggccct gcgctttcgc ggcatccggc tcaccctgcc tgtgcagggg gaggacgcgg
     3061 cggacaccgc gctgttttac ggcaaattca gcgcctggtt ttacgcgggt gtcgccacac
     3121 tgcaaaattt tctcgatctg cgcttcgacc acattgaatt gatccccgac tttgcgggcg
     3181 agaataaata ccgcagaagt ttctattgta aaataggagc cacaccgttt ataatgttag
     3241 tggtggcttt caaggcgttc ggcctgctgc ggcgcgacgg cctgttgccg ggctctggcc
     3301 ccggcgaaaa gaaagcgccg ccatcacaag agggaacggc tgccgcgccc cgcgcgggcg
     3361 gcgcagatat tggaggtagt gaaaatgagt gagcatcccc ttcagggcct gatggacgtc
     3421 gccctgcaga gaatcaagga gatggtggac agcaatacca tcatcggcga gccgatcacc
     3481 atggcggacg ggacgctgat cctgccggtg tccaaggtga gcttcggctt tgcctccggt
     3541 gggtctgatt ttccctcaaa gacgcccaag gagctgttcg gcggcggctc gggcgcgggc
     3601 gtttccatct cgcccatcgc ttttctcgtc gtcaaggacg gcaacgtcaa gatgctgcag
     3661 ctcgcctcac aggaggccaa cgccaccgac cgcatcatca acgcgctgcc ggacgtgctg
     3721 gacaagctgc aggacatgtt caaaaaagaa aaaccgggcg cggacggcca gggctgaggc
     3781 ccgggcttcc cgccggcaac gagccgtctg ccggtggcag gcggcttgtt atgtccggga
     3841 gggacggctg ccgcaacgca aatggttttc gaactaaagg aggaacctcg tgagagatac
     3901 caggattcaa aaagtactgt ccgagctggg cgtctgctcc cgccgcgcgg cggaggagat
     3961 gatccgccag ggcagggtga agctgaacgg caggcccgtt caggtggggg acaagatgga
     4021 caccgcaaag gacctgatct cggtggacgg caggaatatc cgtgtgccga aaaagcagga
     4081 gttcgtctac gaaatgctgt ataagccccg cggctatatc accaccatgt cggacgagcg
     4141 gggccgcaag accgtgatgg agctggtgaa agacgtgccg gcgcgcatct tccccatcgg
     4201 gcggctggac aaggactcgg aggggctgct gctcttcacc aacgacggtg agttcgcaaa
     4261 cctgatgatg cacccctccc acgg
//
LOCUS       DBMDYA010000173         4259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4935_length_4259_cov_1.554353, whole genome shotgun sequence.
ACCESSION   DBMDYA010000173 DBMDYA010000000
VERSION     DBMDYA010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_4259_cov_1.554353"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="ACZPFK_05505"
     CDS             complement(<1..298)
                     /locus_tag="ACZPFK_05505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1405799.1"
                     /translation="MAFIYDEPSHTFGEYLIIPGYSSCECVPSAVNLKTSVVKYRKGE
                     EECPLTMNIPLVSAIMQSVSDDKMAIALAKEGGLSFIYGSQSIEDEAKMVARVKS"
     gene            517..651
                     /locus_tag="ACZPFK_05510"
     CDS             517..651
                     /locus_tag="ACZPFK_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405800.1"
                     /translation="MKGKIFSIVGLVMGCISVSISVTALVFSAIGMVKSKKIKAPRSF
                     "
     gene            complement(960..2261)
                     /locus_tag="ACZPFK_05515"
     CDS             complement(960..2261)
                     /locus_tag="ACZPFK_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1405801.1"
                     /translation="MALLDRISNTNAQVILSLAAMLLAGFLATRVTKKLRLPNVTGYI
                     LAGVAIGPWALHVIPDSVMGGMDFVTDVALAFIAFGVGKYFKLSELRKNGLRIIVVTL
                     FESLAAGLLITLVMYFIFHYSLAFALLLGAIGCATAPASTIMTIRQYKAKGEFVNTIL
                     EVVALDDAVALIAFSIAAAYVGAVESSGAVDPKLVVLPILYNLGGILAGALVGFFLHL
                     IADPERRSAEHRLALVITLIFILTGLCTSLDISPLLSCMVMGTVYVNAGGNKKTFKMV
                     TRFTPPILLLFFVLSGMRLSLPALQSAGLVGVVYFFVRILGKYAGAWAGCAVTGAPKP
                     TRDWLGLALIPQAGVSIGLAVLGQRMLPGPTGVLLTTIILSSGVLYEMIGPACAKASL
                     FLSGTLQRQPRPQPHGGEAKPLPGKALKNTKPAKAVKKAAS"
     gene            2522..3409
                     /locus_tag="ACZPFK_05520"
     CDS             2522..3409
                     /locus_tag="ACZPFK_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1405802.1"
                     /translation="MIDPKIRTLIRVIEEGSFTRAAAALHLTQPAVSHHIRQLEAEYG
                     IPIFRPDKKELVPTPEGAVLIKYARRALALDRSARQAIEDSRRSLRHLAVGLTPTAGE
                     NLMPQVLALYCHEHPETHINIVTDTINNLYQRMELYELDLAVVEGALPGGRFHSVLLD
                     TDYLCLIVSPQHPFARRQSILPGELKGERLILRPAKAGTRALFDSYLQSQGESVQNFN
                     VMMELDNVAMIKDLVSQDLGVSIIAKSACREEERAGRLVAVPIENASMSRNIQLVYHS
                     DFSHPDVLEELRAIYNRIR"
     gene            complement(3953..>4259)
                     /locus_tag="ACZPFK_05525"
     CDS             complement(3953..>4259)
                     /locus_tag="ACZPFK_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR1405803.1"
                     /translation="ISGGATNSYLNLSLFFAFAAVYPNMQVLLFFLIPIKIKWLAIAD
                     GVLFAFQLIFGTWQTRAVIIASLINFLLFFGPDVLRKMRDNFKYRKQREEWRRQMRG"
BASE COUNT          908 a         1231 c         1348 g          772 t
ORIGIN      
        1 agctcttcac acgagccacc attttggcct catcctcgat tgactgcgaa ccatatataa
       61 aagaaagacc cccttctttt gcaagcgcaa tcgccatttt atcgtcggag accgactgca
      121 tgatggcgga gacaaggggg atattcatag taagggggca ttcctcttcg cctttgcggt
      181 acttcaccac cgaggtcttg aggttgacgg cggagggcac gcattcgcag gaggaatacc
      241 ccggtataat gagatattcg ccaaaggtat gagacggttc atcgtaaata aaagccatag
      301 tttcttctcc tttactttga tcgtttcctc ggttgttcca ctggattatt cataataata
      361 ttctttttgc atctggcttg tcaaggcaaa aatgtccgtg gatgcaaaac accgcaaaac
      421 aactaaaagc tgcttgccgc gggcggccgt gactggtata ataacggtag aaatcaaccc
      481 gggccatggc ccggctgagg aaaggagccg ttttgcatga aagggaagat cttcagcatc
      541 gtgggcctgg tgatgggctg catctcggtc tcgatctcgg tgaccgccct ggtcttcagc
      601 gccatcggca tggtcaagtc caaaaagatc aaagcgccgc gcagcttctg agcgcgggcg
      661 tttctccgca aagggagggg ggacggcgga tgccgtctcc ccccttttta tattataaag
      721 gtatcccggg gcgggcaggg aagccgggcg gcaagcgctg ccgcgcggga acgccaattt
      781 tgcccggcgg ccgcagcgcg actcgcggcg tgtgaaacat agaaagggag catggaaaaa
      841 agttggcggc ccgcagtgag ccgccaactt ttttgtaaac tgcatttttt cagaagccgc
      901 cgctccaaaa ccgccgcgcc tcccagggcg gaaaagcgct cttgcagaac cgccgcgggt
      961 caggaggcgg cctttttgac ggcctttgcc ggctttgtgt ttttcagcgc cttgccgggc
     1021 aggggctttg cctcgccgcc gtggggctgg ggacgaggct gccgctgcag tgtgccggac
     1081 aggaacaggg aggccttggc gcaggccggg ccgatcatct cgtacagcac gccggaggaa
     1141 aggatgatgg tggtgagcag cacgccggtg gggccgggca gcatgcgctg gcccaacacg
     1201 gccaaaccga tggagacgcc cgcctggggg atcagcgcca gccccagcca gtctcgggtc
     1261 ggcttggggg cgccggtgac ggcgcagccg gcccatgcac cggcatactt gcccaggatg
     1321 cgcacgaaaa agtagaccac acctaccaga ccggcgctct gcagggccgg cagggacagc
     1381 cgcatgccgg acagaacgaa aaagagcagc aggatcggcg gggtgaagcg ggtgaccatc
     1441 ttgaatgtct ttttgttgcc gcccgcgttg acataaacgg tgcccatcac catgcaggag
     1501 agcagcggcg agatgtccag gctggtgcaa aggccggtga ggatgaagat cagggtgatg
     1561 accagcgcca gccggtgctc ggcggagcgg cgctccgggt cggcgatgag gtgcagaaaa
     1621 aagcccacca gcgccccggc caggatgccg cccagattgt agaggatggg cagcaccacc
     1681 agcttggggt ccaccgcgcc ggagctctcc accgcgccca cataggcggc ggcgatgctg
     1741 aacgcgatca gggccaccgc gtcgtccagc gccaccacct ccagaatggt gttgacgaat
     1801 tcgcccttgg ctttgtattg gcggatcgtc atgatggtgg aagcgggcgc cgtggcgcag
     1861 ccgatggcgc ccagcagcag cgcaaaggcc agggagtagt ggaagatgaa atacatcacc
     1921 agcgtgatca gcagcccggc ggccagcgac tcgaacaggg tgaccacgat gatgcgtagc
     1981 ccgtttttgc gcagctcgga cagcttgaaa tatttgccca cgccgaaagc gataaaggcc
     2041 agcgccacat cagtgacaaa gtccatgccg cccatgacgc tgtccgggat gacgtgcagc
     2101 gcccagggcc cgatggcgac cccggccagg atatagccgg tgacgttggg caggcgcagt
     2161 tttttggtca cgcgggtggc gagaaaacct gccagcagca tggcggccag ggagaggatg
     2221 acctgcgcgt tggtgtttga gatgcggtcc agcagtgcca agacgcatcc ctcctttcaa
     2281 atagaggatg aaaaaagccg cgcggctcac cccgccgcca cgacaaaaag tgagaaaaag
     2341 acaaaaacca aaagaaattt tacaagaact ttacatttct tttgggtatg ccttgattat
     2401 atgagggtaa agtgctaata tcaaattatg attcgttaca agacgataaa aattatttat
     2461 ggataatgga atacttgcac aaaaaatgga ctttgctgga tgaaaaagga ggcgctttgc
     2521 tgtgatcgac cccaagatca gaaccctgat ccgcgtgatc gaggagggca gctttacacg
     2581 ggccgcagcc gccttgcacc tcacccagcc cgcggtcagc caccacatcc gccagctgga
     2641 ggcggaatac ggcatcccca tcttccgtcc ggacaaaaaa gagctggtcc ccacccctga
     2701 gggggcggtt ttaatcaaat atgcgcggcg ggcgctggcg ctggaccggt cggcccggca
     2761 ggcgatcgag gacagccgcc gcagcctgcg ccacctggcg gtgggcctga cccccaccgc
     2821 gggggaaaac ctgatgccgc aggtgctggc gctgtactgc cacgagcacc ccgagaccca
     2881 tataaatatt gttacggaca ccataaacaa tctttatcag cgcatggagc tgtacgagct
     2941 ggatctggcg gtggtggaag gggcgctgcc cggcggccgt ttccactcgg tgctgctgga
     3001 caccgattat ctctgcctga ttgtctcccc tcagcatccc tttgcccgtc gccagagcat
     3061 cctgcccggc gagctgaaag gcgagcggct catcctgcgc ccggccaagg cgggcacccg
     3121 cgccctgttc gacagctacc tgcagagcca gggcgagtcg gtgcagaact tcaacgtcat
     3181 gatggagctg gacaacgtgg ctatgatcaa ggatctggtc agccaggacc tgggggtgtc
     3241 gatcatcgcc aaaagcgcct gccgggagga ggagcgcgcc ggccggctgg tggcggtgcc
     3301 catcgaaaat gccagcatgt cccgcaacat ccagctggtc taccacagcg acttcagcca
     3361 cccggacgtg ctggaggagc tgcgggctat ctacaaccgc atccgctgag tcgcccgcgc
     3421 gggttttccc cgccggcggg gatggcgagc accggcccgg gccggcacgg taagcacagg
     3481 cttcggcggg tgccgcgcga cgcctgcgcg gggttcctcc tggcaggtgc cgttccatgg
     3541 ggcgaggggc gtcgcgggat tgcccgcccc tgcgctgttt ctccccgctg cagctgcgtc
     3601 gtctttcgcc tttttgaatg ctcctgggcg gggcgcacgc aggggaacgg gagagaagct
     3661 tcccaaaagg gcacagggaa accgcaaccc tttctgggtt ctgcggctgc gcggcggcct
     3721 ggaagcgttc cgtgtacaaa ttttttcgca gaaaagccgc tcctctgcgc caaagcgttg
     3781 cagcgccccg ccccgcggag acggaaaggc aaaaaaaggc cccatccggt atcagctcgg
     3841 atggggcctt tttgtttttg atttcttgac gctctgtacc cggccgatgc ggccgcggcg
     3901 gcaaacaggg gcgctttgcc gcgccgggac ggggccgggc gctttcgggc gctcacccgc
     3961 gcatctgccg ccgccattcc tcccgctgct tgcggtattt aaagttgtcc cgcatcttgc
     4021 gcagcacgtc ggggccgaaa aagagcagaa aattgatcag cgaggcgatg atgacggccc
     4081 gggtctgcca ggtgccgaag atcagctgaa aagcgaacaa taccccgtcc gcgatggcca
     4141 gccatttaat tttgatcggg atgaggaaga agagcagaac ctgcatgttg gggtaaaccg
     4201 cggcaaaggc gaaaaacagg gagaggttga gatagctgtt ggtcgcgccg ccggagatg
//
LOCUS       DBMDYA010000174         4257 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4938_length_4257_cov_1.737247, whole genome shotgun sequence.
ACCESSION   DBMDYA010000174 DBMDYA010000000
VERSION     DBMDYA010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4938_length_4257_cov_1.737247"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="ACZPFK_05530"
     CDS             <1..484
                     /locus_tag="ACZPFK_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405804.1"
                     /translation="AGIGEGAATNLANIFLFLFCLFFNRRRIRGATLLCALASGPCVD
                     AGAAALARLFPHPLSPAGRLGVGAASCVLLGLGVGLYLALDFGAAPLDGFVLWLQDVT
                     RLRYRTCTWAFNLVLFAVGIALGGTLGPVTLLGLLVPGAVADVLLTRLGPPRPGPAAP
                     "
     gene            complement(592..1533)
                     /locus_tag="ACZPFK_05535"
     CDS             complement(592..1533)
                     /locus_tag="ACZPFK_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405805.1"
                     /translation="MSIDWVAFIADAIRMATPLVFAALAALITRQAGMFNMGVEGMIL
                     CSALGAVVFAHFGGSVWIGLLGGILIGLVTGMIIAFSSLTGKTDLYLTCIAFNLMAAG
                     GTVFVMYLLTGDKSSTAGSLRSYQLPNFDIPLIKDIPVLGRIISGQSLLTYLAVLSVF
                     LVWFLLKKTRIGLRIRAVGENPSAAESVGISVPKIGYISFAFCGVFAGLSGAFLSMSW
                     VTFFMKNMVNGRGYIGLSAQNMAGGAPVGSALASLLFGFADSLSITLKNQTTFPTEFL
                     NMIPYLATILAIIVTSIIAINKKRRIEKGTAKVAAEK"
     gene            complement(1556..2653)
                     /locus_tag="ACZPFK_05540"
     CDS             complement(1556..2653)
                     /locus_tag="ACZPFK_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405806.1"
                     /translation="MNKTQKIESVMEVVRILVSLILAYGVALIILFAISDEPVFIVQQ
                     FIAGPFSSARRIGSIINLAVPFTICGLSMCFMYAVNKFNLIGEGIFMLSACMVTWVSL
                     SLQSLNLPAAVMVPIMYVVAILTGVVMAFIPAIMDRKFNANVVVVSLMLNSVIAYLAT
                     WILRYKVRDSGIAYMGSKEIPKSVQLPGIFGKFRIQSGLIVAVICVVIIAVLFYKTSF
                     GWKMRLVGANPRFAKAVGLSALGISFAAQLAGGAVAGIAGATEMMSNYTRFQWTTTTQ
                     HGFDGLLVAVLAKKNPVLVPIGALFLAYIRIGADVVNTSGDIPIEFVTVIQGIIILLV
                     AAESFLSGTKNKLIFKAAEKDEKEKQAKAAA"
     gene            complement(2650..>4257)
                     /locus_tag="ACZPFK_05545"
     CDS             complement(2650..>4257)
                     /locus_tag="ACZPFK_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405807.1"
                     /translation="STAGLLPALLKKMKKGVHCVADEVLRMQNITKIYPNGFVANKDI
                     TFGVGANEIHALVGENGAGKTTLMKILFGMEAPQEGKIFINGKEEKIANPLDAIDKGV
                     GMVHQHFMLVPSLSVAENVTLGVEPMKKGLFDFEGAVKATQEIADKYNFKVDARSKVQ
                     DLSVGQMQKVEILKALIKGAKILILDEPTAVLTPQETEELFEQLFTLRDSGVSIIFIS
                     HKLEEVMRICSKVTVLRHGQCMGTHDTEGLNEASISKLMVGRDVVLKIEKEDPKTGKP
                     VISIKNLVKINSIGKHVIDDISFDIHEGEVVGIAGVEGNGQTELSEVLAGLSDFASGD
                     VQLNGASIQGKSVREIRDLGMAYIAEDRMVEGVAGDMSIKMNIMADRFVKKEFKKGIF
                     MNAKKIDETVNQYIKDFEILCDGPDQPVRMLSGGNIQKVVVAREFTSGANFILANQPT
                     RGIDVGTAEMIRKTIVRKSREEGTATLLISADLNEVLECSDRLLVMHKGKIVAAFPKA
                     NEVSEETLGEYMLGIKEMTLEEMEALL"
BASE COUNT          763 a         1262 c         1365 g          867 t
ORIGIN      
        1 cgcgggcatc ggcgagggcg ccgcaactaa tttagcaaat atcttcttat tcctgttctg
       61 cctctttttc aaccgccgcc gcatccgcgg cgccaccctg ctctgtgccc tcgcctccgg
      121 cccctgtgtg gacgccgggg cggcggcact ggcccggctg ttcccccatc ccctctcccc
      181 ggccgggcgc ctgggggtgg gcgccgcgtc ctgcgtgcta ctggggcttg gggtggggct
      241 ctatctggcg ctggatttcg gcgcggcgcc gctggacggc tttgtgctgt ggctgcagga
      301 tgtcacccgc ctccgctacc gcacctgcac ctgggcgttc aatctggtcc tttttgcggt
      361 gggcatcgcc ctgggcggca cgctggggcc cgttaccctg ctggggctcc ttgtccccgg
      421 ggcggtggcc gatgttctcc tcacccgcct ggggccgccc cggcccgggc cggccgcccc
      481 ttgaagcggc gcgcgcgttg cagcacaaaa aggaccgcgc aggttttctg cgcggccctt
      541 ttctctttgt gacgatacac aatgccggaa ctgctcccgc ttggagggag attatttctc
      601 ggcggccacc ttggcagtgc ccttttcgat gcggcgcttc ttgttgatgg cgatgatgct
      661 ggtcacgatg atggccagga tggtggccag gtaggggatc atgttcagga actcggtggg
      721 gaacgtggtc tggttcttca gtgtgatgga cagcgagtcc gcaaagccga agagcaggga
      781 ggccagcgcg gagcccacag gggcgccgcc cgccatgttc tgggcggaaa ggccgatgta
      841 gccgcggccg ttgaccatgt tcttcatgaa gaaggtgacc cagctcatgg agaggaacgc
      901 gcccgacagg ccggcgaaaa cgccgcagaa cgcaaaggaa atgtagccga tcttcggcac
      961 cgagatgccc accgactcgg cggcgctggg gttctcgccc acggcgcgga tgcgcaggcc
     1021 gatgcgggtc tttttgagca ggaaccacac caggaacacc gagagcaccg ccagataggt
     1081 gagcaggctc tggcccgaga tgatgcggcc cagcaccggg atatccttga tgagggggat
     1141 atcgaagttc ggcagctgat agctgcgcag ggagccggcg gtggaggact tgtcgccggt
     1201 gagcaggtac ataacgaaca cggtgccgcc tgcggccatc aggttgaacg cgatacaggt
     1261 caggtagagg tcggtcttgc cggtcaggct ggagaacgcg atgatcatgc cggtgacaag
     1321 accgatcagg ataccgccca gcaggccgat ccagacgctg ccgccgaagt gggcaaagac
     1381 cactgcgccc agggcggaac ataggatcat accttcaacg cccatgttga acatgcccgc
     1441 ctggcgggtg atcagggcgg cgagggcggc gaagaccaga ggggttgcca tgcggatggc
     1501 gtctgcaata aatgcgaccc aatcaatact cataatgctg tcttctcctt tcgtttcagg
     1561 ctgctgcctt cgcctgtttc tctttttcgt ccttttcggc agccttgaag atcagcttgt
     1621 tcttggtgcc ggagaggaag gactcagccg cgaccaggag gatgatgatg ccctggatga
     1681 ccgtgacgaa ctcgatgggg atatcgccgc tggtgttgac cacgtccgcg ccgatgcgga
     1741 tgtaggccag gaacagcgcg ccgatgggca ccagcaccgg gttcttcttg gcgagcacgg
     1801 ccaccagcag gccgtcaaag ccgtgctggg tggtggtggt ccactggaag cgggtgtagt
     1861 tgctcatcat ctcggtggcg ccggcgatac cggcaacagc gccgccggcc agctgagccg
     1921 caaaggagat gccgagggcg gagaggccca cagccttggc gaaacggggg ttcgcgccca
     1981 cgaggcgcat cttccagcca aaggaggtct tgtagaacag caccgcgatg atcaccacgc
     2041 agatgaccgc cacgatcaag ccggactgga tgcggaactt gccgaagatg ccgggcagct
     2101 gcacgctttt cgggatctct ttggagccca tgtaggcgat gccggagtcg cggaccttat
     2161 agcgcaggat ccaggtggcc agataggcga tgacgctgtt gagcatcagc gagaccacga
     2221 ccacgttggc gttgaacttg cggtccatga tggcggggat aaaagccatc accacaccgg
     2281 tgaggatagc caccacgtac atgatgggca ccatcacggc ggcgggcagg ttcaggctct
     2341 gcaggctcag ggagacccag gtgaccatgc aggcggagag catgaaaatg ccctcgccga
     2401 tcaggttgaa cttgttgacc gcatacatga agcacatgga caggccgcag atggtgaacg
     2461 gcacggccag gttgatgata ctgccgatgc gccgggccga ggagaacggg cctgcgatga
     2521 actgctgcac gatgaaaacg ggctcgtcgc tgatggcgaa caggatgatc agggccacac
     2581 cgtaagcgag gatcaagctc accaggatgc ggacgacctc cattacggat tcaatttttt
     2641 gtgtcttatt cataacaggg cctccatctc ttccagagtc atttccttga tgcccagcat
     2701 gtactcgccc agcgtctcct ccgagacctc gttggccttg gggaaagccg cgacgatctt
     2761 gcccttgtgc atgacgagca gccggtcaga gcactccagc acctcgttca ggtcggcgga
     2821 gatcagcagg gtggcggtgc cctcctcgcg ggatttgcgc acgatggtct tgcggatcat
     2881 ctcggcggtg cccacgtcga tgccgcgggt gggctggttg gcgaggatga agttggcgcc
     2941 gctggtgaac tcgcgggcga cgaccacttt ctggatgttg ccgccggaga gcatgcgcac
     3001 cggctggtcc gggccgtcgc agaggatctc aaaatccttg atgtactggt tgaccgtctc
     3061 gtcgatcttc ttggcgttca tgaagatgcc tttcttgaac tcctttttga cgaagcggtc
     3121 ggccatgatg ttcatcttga tggacatgtc gcccgcaacg ccctcgacca tgcggtcctc
     3181 ggcgatgtag gccatgccca ggtcgcggat ctcgcgcacc gatttgccct ggatggaggc
     3241 cccgttcaac tggacgtcgc cgctggcgaa atcgctcagg cccgccaaaa cctcggagag
     3301 ctcggtctgg ccgttgccct cgacgccggc gataccgacg acctcgccct cgtggatgtc
     3361 aaagctgatg tcgtcgatga catgcttgcc gatggagttg atcttgacca ggttcttgat
     3421 ggagatgacc ggcttgccgg tcttggggtc ttctttttcg atcttgagca caacgtcgcg
     3481 gccgaccatc agcttggaga tggacgcctc gttgaggccc tcggtgtcat gggtgcccat
     3541 gcactggcca tggcgcagca cggtgacctt gctgcagatg cgcatgacct cctccagctt
     3601 gtgggagatg aagatgatgg acacgccgct gtcgcgcagg gtgaacagct gctcgaacag
     3661 ttcctcggtc tcctgcgggg tgagcacggc ggtgggctcg tcgaggatga ggatcttggc
     3721 gcccttgatc agggctttca ggatctccac tttctgcatc tgaccgacgc tcaggtcctg
     3781 caccttgctg cgggcgtcca ccttgaagtt gtacttgtcc gcgatctcct gggtggcctt
     3841 gacggcgccc tcaaaatcga acaggccttt cttcatcggc tcgacgccca gggtcacgtt
     3901 ctccgccacc gacagcgagg ggaccagcat gaagtgctga tgcaccatgc cgacgccctt
     3961 gtcgatcgcg tcgaggggat tggcgatctt ctcctccttg ccattgatga agatcttgcc
     4021 ctcctgcggc gcttccatgc cgaacaggat cttcatcaga gtggtcttgc ctgcaccgtt
     4081 ttcgccgacg agcgcgtgga tctcgttggc gccgaccccg aacgtgatgt ccttgttggc
     4141 gacaaaacca ttcgggtaga tcttggtgat gttttgcata cgcagtactt cgtctgccac
     4201 gcaatgtacc ccctttttca tctttttcag cagagcggga agcagccccg ctgttga
//
LOCUS       DBMDYA010000175         4218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4989_length_4218_cov_1.632256, whole genome shotgun sequence.
ACCESSION   DBMDYA010000175 DBMDYA010000000
VERSION     DBMDYA010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_4218_cov_1.632256"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1674)
                     /locus_tag="ACZPFK_05550"
     CDS             complement(1..1674)
                     /locus_tag="ACZPFK_05550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HIR1405808.1"
                     /translation="MGMYSNERVEGFLHTDGRSMRNGRGEEVRLRGWAAGNWTNPEGF
                     LCGDVRRPWAMDDPAQVPARFDRARSIEDSVRLLCGSAYAKGFWPRWYRSHLGEADLR
                     AMAAYGYNSVRLPLNARAFLPEEPGFAWNEDSFAMLDEVLGWCEKYRLYAILDLHTAP
                     GGQSGIKCDDGLKSMPLFFLEEESMERSIRLWEEIARRYKDRWIVGGYDLLNEPVAPC
                     EWFQLIPQLAAYYDRLIPRLRAIDKNHMLTIEGAAAATNMEIFDHNYDPKCNNWCIHV
                     HFYGLSPERRSLFRYLEPSLRLNVPVWLGEGGGPAQDIAALLEAADSLGMGFNLWSWK
                     TSMAGGGKLRRDPVQYALPRGWDRLLDFFNGAGPRPGYTECQAIFDEMLENMKFENCT
                     LVPEQHGVVLRRPGRRVPAAAYDSTRGAFAGGWEYGNPYDFRTEDATHLLLRDGVLPP
                     RRDMPFSRPMNFRWDPLDDLLLELDTGAYASYSLLDAQEPCRVRVTVRARERSILHIS
                     CGGMEKLVPLEPGAGFVPVEAFTLPAAEEHRVRVEAVEGRIQLESVEFL"
     gene            1910..2332
                     /locus_tag="ACZPFK_05555"
     CDS             1910..2332
                     /locus_tag="ACZPFK_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405809.1"
                     /translation="MQNRLSEQRGLRQVRGCFRNYLLGGLLLVLLYAAWHRGFTPGSP
                     AGAAWVWGDGFVMSGILLYLAVGASALGRHGYFDWVFYGAYLSKFLFTHGKDEEYTNF
                     FDFKKLRRRGTAPFWPGVLVATIWFLIGLILSMLYYLL"
     gene            2771..>4218
                     /locus_tag="ACZPFK_05560"
     CDS             2771..>4218
                     /locus_tag="ACZPFK_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1405810.1"
                     /translation="MSMKHSSRWHKIAAAALAASLAMGLLTGCGGGDAGGSSAAGGAS
                     GGTVAAGGAKNLTFVMSSDVGDLSPFGGDSGGRHHTYRMIYDCLCASEGLGDTVEELQ
                     GQMAKTVTVVDNSTVDVELYDYIHDCQGNPIKASDVVFSYEAAIASGTMEKLNGYLDK
                     VEETGDYSVRITLSSPSKGAMEWCLTTVPVISQSWFESASDADKTSHPATTGAYNLVE
                     TVASSHTTLQKNADYWQTDESLRAYYDVQTFDTINMNVVTESAMRTIALQNGEADLVQ
                     NVSANEISSFLNADGSNVEGWNVFSNENGRMNVLMFNNDNSVFADNKELRQAVLYGID
                     FEAVRQGYGNTATNGSVCSSFGPDIAGDFNPEWKNEDYYGYDVAKAKELMAKAGYPDG
                     GFTVKLLYQNSTTATAGLTVLQAYLAELGITVELTPADQALFNSYKYDDTKWDMIADS
                     KGVDYITAGWENVFDARAFENGSACFTHDDKL"
BASE COUNT          791 a         1394 c         1271 g          762 t
ORIGIN      
        1 tcatagaaat tcgacgctct ccagctggat gcgcccttcc acagcctcca cgcgcaccct
       61 gtgctcctcc gccgcgggca gcgtaaaggc ctccacgggc acaaacccgg cgcccggctc
      121 cagcgggacc agcttttcca tgccgccgca gctgatgtgc aggatgctgc gctcccgcgc
      181 gcgcaccgtc acccgcaccc ggcagggctc ctgcgcatcc agcagggaat aagaggcgta
      241 ggcccccgtg tccagctcca gcagcaggtc gtccagggga tcccagcgga agttcatcgg
      301 ccgggaaaag ggcatgtccc gccggggcgg cagcacgccg tcccgcagca gcagatgggt
      361 tgcgtcctcg gtgcggaaat cataggggtt gccgtattcc cacccgccgg caaaggcgcc
      421 ccgggtggag tcgtaggcgg cggccggcac ccgccgcccc ggccgccgca gcacgacgcc
      481 gtgctgctcg ggcaccagcg tgcagttttc aaatttcatg ttctccagca tctcgtcgaa
      541 gatggcctgg cactcggtat agccgggccg cggcccggcg ccgttgaaaa agtccagcag
      601 ccggtcccag ccccggggca gcgcgtactg caccgggtcc cggcgcagct tgccgccccc
      661 ggccatcgag gtcttccagc tccagaggtt gaagcccatc cccaggctgt ccgccgcctc
      721 cagcagcgcc gcgatgtcct gggcggggcc gccgccctcg cccagccaga ccggcacgtt
      781 cagccgcagg ctgggctcca gatagcggaa cagcgagcgc cgctccggcg agaggccgta
      841 aaagtggaca tggatgcacc agttgttgca cttggggtcg tagttgtggt caaaaatctc
      901 catgttggtg gcggcggccg ccccctcgat ggtgagcatg tggtttttgt cgatggcccg
      961 cagccgcggg atgagccggt cataataggc cgccagctgc gggatgagct ggaaccactc
     1021 gcagggggcc accggctcgt tgagcaggtc gtagccgccc acgatccagc ggtccttata
     1081 gcgccgggcg atctcctccc acaggcggat cgaccgctcc atgctctcct cctccaaaaa
     1141 gaaaaggggc atggatttca gcccgtcgtc gcacttgatg cccgactggc cgcccggcgc
     1201 ggtgtgcagg tccaggatgg cgtacagccg gtatttttca caccagccca gcacctcgtc
     1261 cagcatggca aagctgtcct cgttccaggc aaagcccggc tcctctggca aaaaggcccg
     1321 cgcgttcagc ggcagccgca ccgagttgta gccgtaggcc gccatggcgc ggagatccgc
     1381 ctcccccaga tggctgcggt accagcgggg ccaaaagcct ttcgcgtagg cgctgccgca
     1441 cagcagccgc acactgtcct cgatgctgcg ggcgcggtcg aagcgggccg gcacctgggc
     1501 cgggtcgtcc atcgcccagg ggcggcgcac gtccccgcac aaaaagccct ccgggttggt
     1561 ccagttgccc gcggcccagc cccgcaggcg cacctcttcc ccgcggccgt tgcgcatgct
     1621 gcggccgtcc gtatgtaaaa agccctccac gcgctcattg gaatacatgc ccatccccgc
     1681 acttcccttc catcccgtgt ctcttttgtg gaacttcact actattcagt ataattgacg
     1741 acatttttta ttcaattctc ttttcgtctt acccattatt cctttttaga atattgtgac
     1801 ctttcgcggc agcttccctt gacaggaaaa agcccgccgg gctataatgc tgaccagatc
     1861 agacggcggc gcgccctcgc tttgcccggc ggccgcaaaa ggagcaatca tgcaaaacag
     1921 attgtcggag cagcgcgggc tgcggcaggt ccggggctgc tttcgaaact atcttctcgg
     1981 ggggctgctg ctggtgctgc tgtacgcggc ctggcaccgg ggctttacgc ccggcagccc
     2041 ggccggggcg gcctgggtgt ggggggacgg ctttgtgatg tccggcatcc tcctttatct
     2101 cgcggtgggc gcctcggccc tggggcgtca cggctatttc gactgggtct tttacggggc
     2161 ttatctgtcc aagtttctgt tcacccacgg caaggatgag gagtacacca actttttcga
     2221 ctttaaaaag ctgcgccggc gcggcacggc gccgttctgg ccgggggtgc tggtggccac
     2281 catctggttt ttgatcggcc tgattttgag catgctgtac tatctgctct gaggcgcccg
     2341 cgcgcgccgc aaacatcgtt ttttcctttg gcccgggctt tgcccgggcc tttttgttgt
     2401 ctggcccagc tgtcaaaact tcacaaaaaa agttgatttt tttgacaacg tcgcccaagc
     2461 tcttggacat ttccgctaat ttcacggcgg caaagctgtg caaactctct attccttttg
     2521 ggattattcc ctattggaat tttgtgtctc cggaaaactg aattgaatgg gggcaatgca
     2581 gccgctacaa tgaaatcgca cagaagaatt cccgaaaacc gcttcaaaaa tggggattct
     2641 ccacaagccc gctcccttgc tttttgaccg ggaggggccg ggggcacagc gtaagtagcg
     2701 accgcccgca cctgtggcag agcgcggcgg cctatttata aaaccggcaa gaaaaggaag
     2761 gagaaacatc atgtcaatga aacattcttc acgctggcac aagatcgcgg ctgccgccct
     2821 cgcggccagc ctcgccatgg gcctgctcac cggctgcggc ggcggcgacg cgggcggcag
     2881 ctccgccgcg ggcggcgcgt cgggcggcac agtggcggcg ggaggggcca aaaacctcac
     2941 tttcgtcatg agcagcgacg tgggcgacct ctccccgttc ggcggcgaca gcggcggccg
     3001 ccatcacacc taccgcatga tctacgattg cctgtgcgcc agcgagggcc tgggcgacac
     3061 ggtggaggag ctgcagggcc agatggccaa aacggtcacc gtggtggaca attccaccgt
     3121 ggacgtggag ctctatgatt acatccacga ctgccagggc aaccccatca aggccagcga
     3181 cgtcgtcttc agctacgagg cggccatcgc cagcggcacc atggaaaagc tgaacggcta
     3241 cctggacaag gtggaggaga ccggcgacta cagcgtgcgc atcaccctct cctccccctc
     3301 caagggcgca atggagtggt gcctgaccac ggtgccggtc atcagccaga gctggtttga
     3361 gtccgcctcc gacgcggata agacctcgca ccccgccacc accggcgcct acaacctggt
     3421 ggagaccgtg gccagcagcc acaccaccct gcagaagaac gccgattact ggcagaccga
     3481 cgagtcgctg cgcgcctact acgacgtgca gacctttgac accatcaaca tgaacgtggt
     3541 caccgagtcc gccatgcgca ccatcgccct gcagaacggc gaggccgacc tggtgcagaa
     3601 cgtgtccgcc aacgagatct cgtcgttcct caatgcagac ggcagcaatg tggagggctg
     3661 gaacgtcttc tccaatgaga acggccgcat gaacgtgctg atgttcaaca acgacaacag
     3721 cgtgtttgcc gacaacaaag agctgcgcca ggcggtgctg tacggcatcg actttgaggc
     3781 ggtgcgccag ggctacggca acaccgccac caacggctcg gtctgctcct ctttcggccc
     3841 ggacatcgcc ggcgacttca accccgagtg gaaaaacgag gattactacg gctatgacgt
     3901 ggccaaggcg aaagagctga tggccaaggc cggttacccg gacggcggct tcaccgtcaa
     3961 gctgctgtac cagaacagca ccaccgccac cgcgggcctc accgtgctgc aggcctatct
     4021 ggccgagctg gggatcaccg tcgagctcac ccccgcggac caggccctgt tcaactctta
     4081 caagtacgac gacaccaagt gggacatgat cgccgactcg aagggcgtgg attacatcac
     4141 cgccggctgg gagaacgtgt ttgacgcccg cgcctttgaa aacggcagcg cctgcttcac
     4201 ccacgacgac aagctgca
//
LOCUS       DBMDYA010000176         4216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_4993_length_4216_cov_1.646659, whole genome shotgun sequence.
ACCESSION   DBMDYA010000176 DBMDYA010000000
VERSION     DBMDYA010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4993_length_4216_cov_1.646659"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(13..1239)
                     /locus_tag="ACZPFK_05565"
     CDS             complement(13..1239)
                     /locus_tag="ACZPFK_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1405811.1"
                     /translation="MDMAGAGGSARRKKRGNNFESMQQSNRALVLQLIKAAGVSTRKS
                     LAEQSGLQPATISIIVAELLERRLVEETGLVEGDCGRRLTGLRLRRDRYCTAAVRITP
                     SYYAVGLYDINSACLAVEKNVWPFFEDIAASLQRVLTAVRRFLALRPDLRPLGAALAV
                     QGDFWFDNGRCTMPGFGGEAGRDLCGWLSRALGIPVSADLAADYGAYHFSALPDYAYL
                     QQEVFLNVLVSDTVDYSILDHGHIFRGALGVPGSLGRLIVLDGAGRPTPLEELCGNTV
                     LLRRAAALAHTRSDSALAGREPLRSRDLINAFYAGDPIAVELYGGAAAALAQALAVLI
                     RLLHPHRIFLSDEIPQNEGFLSLVRRALAREIGPRLLEKVRLCAPSGERSTARDGTMI
                     GGSRYLTDRSIRELRL"
     gene            1351..2391
                     /locus_tag="ACZPFK_05570"
     CDS             1351..2391
                     /locus_tag="ACZPFK_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR1405812.1"
                     /translation="MKNRTAYLTAAQTIELRDTEMPRPADDEVLIEVKHVGICGSDVM
                     FYEDPSVGGRLDTPLPVVLGHESAGVVVQTGAAVTGLRPGDRVALEPGIPCGKCRWCL
                     SGKYNLCPQVNFMACSPWERAAFSRYITHPAAFSFKLPDKVSTLEGALVEPLAVGLHA
                     ASRAGAGLGQTAVILGSGCIGLMTLLACKAMGVSRVVMADLFDNRLEIAARTGADLTV
                     NSGREDLVRRVLDFTGGLGADLVFETAGSPVTTALTPELVMRGGRIVLVGNIHEKVQF
                     DFFALNSKEVDVLSIFRYRNIYPMAIQAIAAGAIDVAPIATDLFPFERVAEAFDVAKN
                     QKQTALKVVVEF"
     gene            2404..3231
                     /locus_tag="ACZPFK_05575"
     CDS             2404..3231
                     /locus_tag="ACZPFK_05575"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1405813.1"
                     /translation="MLANFRELLDAAQAGHYAVGAYDLIDMEMLYGILDAAEQTDTPV
                     ILQYAQVFEPTVDISRFARLLVAAAEAARVPVAVHLDHGQDLETVKKCVELGFGSVMI
                     DASSRPFEENVALTRQVVEYCRPLGVPVEAELGHVGEAAQYDLSNYHYTDPGLAAQFV
                     ERTGIDALAVAIGNAHGAYSSTPQINFEVLAAVRGAVSVPLVLHGASGIPDEDIRRAV
                     EGGITKINIFTELTQQAAVELSACAADGYNIFSVSKAIRTGTRDRCLQKIRLFGTRR"
     gene            3255..4202
                     /locus_tag="ACZPFK_05580"
     CDS             3255..4202
                     /locus_tag="ACZPFK_05580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405814.1"
                     /translation="MKELDVLCVGLCCIDVLIRGVDLSTPFDAESKHADSTTLSVGGD
                     ALNEARVLARLGDRVALQCAVGRDVGAQLIRSAVAESGADIRGITAEMDATPMNVVVI
                     QPDGQRNFINTGLLSYGDYDPDPALLSRARVVSLGSLMVPPLHRPERAERIARQAKEA
                     GALVCADCGGLSGGWRLQDFAGVLSQLDFIFPNLEEALSLTGASTAETAAEVLYGFGV
                     KNVVVKTGSRGCYLRSAEGGTAIPTYTHLPVVDTTGAGDNFAAGTIHALLEGKSPAEA
                     CRFGNGVASVAIQVIGASTGVKSLAQVRAAISRFDQGLV"
BASE COUNT          693 a         1425 c         1416 g          682 t
ORIGIN      
        1 gcccgcctcg cctcaaaggc gcagctcccg gatgctgcgg tcggtcaggt acctgctgcc
       61 gccgatcatg gtgccgtccc gggcggtgct ccgctccccg ctgggggcgc acagccgcac
      121 cttttccagc agccgggggc cgatctcccg cgccagcgcc cgccgcacca gcgagagaaa
      181 gccctcgttc tgcgggatct catcgctgag aaagatgcgg tgcgggtgca gcagccggat
      241 caacacggcc agcgcctgag ccagcgccgc cgcggccccg ccgtacagct ccaccgcgat
      301 cgggtcgccg gcataaaagg cattgatcaa atcacggctg cgcagcggtt cccggcccgc
      361 cagcgcgctg tccgaacggg tgtgcgccag cgccgccgcg cggcgcagca gcacggtgtt
      421 gccgcacaac tcctccagcg gcgtgggccg cccggcgccg tccaagacga tgagcctgcc
      481 cagcgagccc ggcaccccca gcgccccgcg aaagatgtgg ccgtgatcca ggatgctgta
      541 atccacggtg tcgctgacca gaacgttgag aaagacctcc tgctggaggt aggcgtaatc
      601 gggcagggcg ctgaaatggt aggccccgta atccgccgcc agatccgcgc ttaccggtat
      661 gcccagcgcg cgggagagcc agccgcagag gtcccggcca gcctcccccc caaagccggg
      721 catggtgcag cgcccgttgt cgaaccagaa gtcgccctgc accgcaaggg ccgcccccag
      781 ggggcgcagg tcgggccgca gggcgagaaa ccgccgcact gccgtcagga cccgctgcag
      841 ggaggccgca atgtcctcaa aaaagggcca gacatttttt tccaccgcca ggcaggcgga
      901 attgatatca tacagcccca cggcgtaata ggagggcgtg atgcgcaccg cggcggtgca
      961 atagcggtcc cgccgcagcc gcagccctgt gagccggcgc ccgcagtcgc cctccaccag
     1021 ccccgtctcc tccaccagcc ggcgctccag cagctcggcc acaatgatgc tgatggtggc
     1081 aggctgcagg ccgctctgct cggccaggct ctttcgggtg gagaccccgg cggccttgat
     1141 gagctgcaac accaaagccc ggttgctctg ctgcatgctc tcaaaattat tgccgcgctt
     1201 tttgcggcgg gccgaaccgc ctgcaccggc catgtccatc tcaagccctc ccctgccgtc
     1261 ccgctgtttt aattcaattt attttatttt agagaaaagc gccgcccctg tcaaacgccg
     1321 ggcggcgccc atggaaagga aggatcgcct gtgaaaaacc gcaccgctta tctgaccgcg
     1381 gcccagacca ttgagctgcg cgacaccgag atgccccgcc ccgccgacga cgaggtgctg
     1441 atagaggtca agcatgtggg catctgcggt tcggatgtga tgttctatga ggaccccagc
     1501 gtggggggcc gcctcgacac gccgctgccg gtggtgcttg gccatgagag cgccggggtg
     1561 gtggtgcaga ccggggctgc ggtgaccggc ctgcgacccg gcgacagggt ggcgctggag
     1621 ccgggcatcc cctgcggcaa atgccgctgg tgcctgtcgg ggaagtacaa cctttgtccc
     1681 caggtcaact ttatggcctg ctccccctgg gagcgcgcgg cgttcagccg ctatatcacc
     1741 caccccgccg ctttcagctt caagctgccc gacaaggtct ccacgctgga gggcgcgctg
     1801 gtggagcccc tggcggtggg cctgcacgcg gccagccgcg ccggggccgg gctggggcag
     1861 accgcggtca tcctgggcag cggctgcatc ggcctgatga ccctgctggc ctgcaaggcg
     1921 atgggggtgt cccgggtggt gatggccgat ctcttcgaca accggctgga aatcgccgcc
     1981 cgcaccggcg ccgacctgac ggtcaattcc ggccgggagg atctggtgcg gcgggtgctg
     2041 gatttcaccg gcggcctggg cgccgatctg gtcttcgaga cggcgggcag cccggtgacc
     2101 accgccctga cgcccgagct ggtgatgcgg ggcgggcgga tcgtgctggt gggcaatatc
     2161 catgaaaaag tacaattcga ctttttcgcg ctcaattcca aagaggtgga tgtgctctcg
     2221 atcttccgct accgcaacat ctaccccatg gcgatccagg ccatcgcggc gggcgccatc
     2281 gacgtggccc ccatcgccac cgacctcttt ccgttcgaac gggtggcaga ggcgttcgac
     2341 gtggcgaaaa atcaaaaaca gacggccttg aaagtcgtcg tggaattctg aggaggtttt
     2401 tttatgcttg caaatttcag agaactgctg gacgcggcgc aggccggcca ctacgcggtg
     2461 ggcgcctatg acctgatcga catggagatg ctctacggca tcctggacgc ggcggaacag
     2521 accgatacgc cggtcatcct gcaatacgcg caggtgtttg agcccacggt ggatatctcc
     2581 cgtttcgcca ggctgctggt ggcggcggcg gaggccgccc gggtgccggt ggcggtgcat
     2641 ctggaccacg gccaggatct ggagacggtc aaaaaatgcg tcgagctggg cttcggctcg
     2701 gtgatgatcg acgcctcctc ccgccccttt gaggagaatg tcgccctcac ccgccaggtg
     2761 gtggagtact gccggccgct gggcgtgccg gtggaggccg agctggggca cgtgggggag
     2821 gcggcgcagt acgacctctc caactaccac tacaccgacc ccgggctggc cgcccagttc
     2881 gtcgagcgca ccggcatcga cgcgctggcc gtggccatcg gcaatgccca cggcgcttat
     2941 tccagcacgc cgcagatcaa ctttgaggtg ctggccgcgg tgcgcggggc ggtgtcggtg
     3001 ccgctggtgc tgcacggggc atccggaatc ccggatgagg acatccgccg cgccgtcgag
     3061 ggcggcatca ccaagatcaa catctttacc gagctgaccc agcaggcggc cgtcgagctg
     3121 tcggcctgcg cggcggacgg ctacaacatc ttctccgtca gcaaggccat ccgcaccggt
     3181 acccgtgacc gctgcctgca aaagatccgc ctgttcggca cccgacgcta gagcggggga
     3241 aaggagcatt tgtgatgaaa gagctcgacg tgctctgtgt gggcctgtgc tgtatcgacg
     3301 tactgatccg cggcgtggac ctctccaccc cctttgacgc cgagagcaag catgcggatt
     3361 ccaccaccct gtcggtgggc ggcgacgcgc tcaacgaggc ccgtgtgctg gcccgcctgg
     3421 gggaccgggt ggccctgcaa tgcgccgtgg ggcgggatgt gggcgcacaa ctgatccgca
     3481 gcgcggtggc tgagagcggc gcggacatcc gcggcatcac cgccgagatg gacgccactc
     3541 ccatgaatgt ggtcgtcatc cagccggacg gccagcgcaa cttcatcaac accggtctgt
     3601 tgtcctacgg cgactacgac ccggaccccg cgctgctgag ccgcgccagg gtggtgtcgc
     3661 tgggcagcct gatggtgccg ccgctgcacc ggcccgagcg ggccgagcgc atcgcgcggc
     3721 aggccaaaga ggcaggcgcg ctggtctgtg cagactgcgg cggcctctcc ggcggctggc
     3781 ggctgcagga ttttgcaggc gtgctctccc agctggactt tatcttccct aacctggagg
     3841 aagccctcag cctcaccggg gcctccacgg ccgagacagc cgcggaggtg ctctacggct
     3901 tcggggtgaa gaacgtggtg gtgaagacgg gcagccgcgg ctgctatctg cgcagtgccg
     3961 agggcggcac ggcgatcccc acctacaccc atctgccggt ggtggacact accggcgccg
     4021 gggacaactt tgccgccggc accatccacg cgctgctgga gggcaaaagc cccgccgagg
     4081 cctgccgctt cggcaacggc gtggcctcgg tggccatcca ggtgatcggc gcctccaccg
     4141 gtgtcaagag ccttgcccag gtgcgcgcgg cgatctcgcg gttcgaccag gggctcgtct
     4201 gagctcctgc atcggc
//
LOCUS       DBMDYA010000177         4189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5024_length_4189_cov_1.273973, whole genome shotgun sequence.
ACCESSION   DBMDYA010000177 DBMDYA010000000
VERSION     DBMDYA010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_4189_cov_1.273973"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(352..438)
                     /locus_tag="ACZPFK_05585"
     tRNA            complement(352..438)
                     /locus_tag="ACZPFK_05585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(402..404),aa:Leu,seq:taa)
     gene            614..1099
                     /locus_tag="ACZPFK_05590"
     CDS             614..1099
                     /locus_tag="ACZPFK_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405815.1"
                     /translation="MCHSKCSPRACVCIIAVVAVLAAIAGGLLYAFLPLPYLRFAVIA
                     MLVLSAAALLCTGAMLTRVCTCGCSVPCLCPCGKAIPITAAGTVAVTLFLLAREFEQS
                     TPQATVAASVNILNAILAALATGFFAAMLLALVWYMVCAFKVVSCRPRPQPCYNDGSN
                     G"
     gene            1209..2159
                     /locus_tag="ACZPFK_05595"
     CDS             1209..2159
                     /locus_tag="ACZPFK_05595"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1405816.1"
                     /translation="MREHRVNAVRPQRLADYLPSAFPLLTPGKVYRFAKEKKIRCNGA
                     HCDGAHRVRQGDVLALYLPEELLRPQEGPAYLRAREALRVVYEDAQLLVADKPAGLLV
                     SDEEGGEADTLLNRALRHLAESGDWEPQDAFTPCLCHRLDAGTSGLVMIAKTPFCRDA
                     VLEAMRLRQFKKTYLGVTFGRPAPPSGTLRGYLSKDAARGLVRVTSAPRPRTREIVTG
                     YRTLAVSGRLALLEIDLVTGRTHQIRAHLASIGCPLLGDSKYGNQAANRELRAKYQLL
                     TAWRLQLPQFGPGPLQQLSGRVLTAEKPWYYAQVLDHTLK"
     gene            complement(2679..3530)
                     /locus_tag="ACZPFK_05600"
     CDS             complement(2679..3530)
                     /locus_tag="ACZPFK_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HIR1405817.1"
                     /translation="MSGEKELRESLFCCPVCGGPLRDGGRALRCPAGHSFDRAAQGYV
                     NLLPANKKHSAVPGDGKGMVAARRAFLEAGWYAPFSEALGRIASACLEEVQAPVVLDA
                     GCGEGYYTGRLAQVLGPRARIAAFDISKEAVRLAAQRYKGVEFAVAGVFAIPMADGAA
                     DCLVDIFAPMAQAEFLRVLRPGGFLLYAVPGPRHLFGLKRVLYEQPYENPVRQTDYPG
                     FCFCRRVPVSGSIALSGGAEIGALFAMTPYYWKTPRGGAEKLEQLNTLQTEIEFDFLI
                     YQKKKEP"
     gene            complement(3582..>4189)
                     /locus_tag="ACZPFK_05605"
     CDS             complement(3582..>4189)
                     /locus_tag="ACZPFK_05605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1405818.1"
                     /translation="LWEALPHDVCPEEALHRRLGHACLPWWTMPGRATPTGDAWWAYV
                     QDGFNTLLQECGFDCRTAARAVAALRPKLRDPSRHRLYPDAVPVLAELQRRGYRSFLL
                     SNNFPELWEVAQKLGLAPYFSGHIVSGEVGWDKPAREIFEAARQLAGLPQSAVMIGDS
                     VGDDIEGAKNAGLQAILVHSEPDGRADACCPQLTGLLELLP"
BASE COUNT          770 a         1436 c         1239 g          744 t
ORIGIN      
        1 acgcaaagct ataaaaacgg ccacgtgggc cgtcccggcc cacggcctta aaatctaagc
       61 gcccgcccac gggagtgggc ggatgcgcga aaaaatagcc tgcggctatt ttttataata
      121 tcatgccgcg cattccacca agagcaaggt gtaaaccggc ttttcatggg gtttctcaat
      181 atgaaaaaaa cctcgcagag ttcaccctgc gaaagcgctt ttggtagagg atagactcgc
      241 tttcctgcaa aaagccacgg cggttgagac ccatacccgg ctggccgccg atagccgtct
      301 ttcgaacccc atctcaaagc aaaaaaatag aagcgccaaa ggcgcttcta ttggtgccgc
      361 tgaccgggtt tgaaccggta cggtgtttcc accgagggat tttaagtccc ttgtgtctgc
      421 caattccacc acagcggccc gggtcacgga cgacggaacg tttattatat tacaatattg
      481 ccgccaaaaa gtcaatttgc ccctgcggcg gcccacacgg cagccggcgc cctctctgca
      541 cggttttgcg acccggcgca tatggtgagc tgacaagtgc tcttgtcaaa ttcatttttt
      601 tcaggaggaa gatatgtgcc attcaaaatg tagtccccgc gcctgcgtct gcatcatcgc
      661 cgtcgtggcg gtgctggcgg ccattgccgg cgggttgctg tacgcttttc tgccgttgcc
      721 ctatctgcgc ttcgcggtga tcgccatgct ggtcctgtcc gccgccgccc tgctctgcac
      781 cggcgcgatg ttgacccgtg tgtgcacctg cggctgctcg gtgccctgcc tttgcccctg
      841 cggcaaagcc atccccatca cggctgcggg cacggtcgcc gtcaccctgt tcctgctcgc
      901 gcgggaattt gagcagtcca ccccccaggc gacggtcgcc gcctcggtga acatcctcaa
      961 cgccatcctc gcggcgctgg ccaccggctt tttcgccgcc atgctgctgg ccctggtgtg
     1021 gtatatggtc tgcgctttta aggtggtctc ctgccgcccc cgcccgcagc cctgctacaa
     1081 cgacggctcc aacggctgaa caatcgcccg gcatggggcg gaccccggtc cgcccctttg
     1141 ctttgcccgt aggcctgtgt tatactgaag cagttccaag ccgccatgca aaaaaaggag
     1201 ggcgaaccat gcgcgaacac cgggtgaacg ccgtcaggcc acagcggctg gcagactatc
     1261 tgcccagcgc ttttccgctt ttgacccccg gcaaggtgta ccgcttcgca aaagaaaaaa
     1321 agatacgctg caacggcgcg cactgcgacg gcgcgcaccg ggtgcggcag ggcgacgtgc
     1381 tggccctcta tctgccggag gagcttctcc gtccacagga ggggccggct tatctgcgcg
     1441 cccgggaggc gctgcgcgtg gtctatgagg acgcgcagct gctggtcgcg gacaagcccg
     1501 ccggcctgct ggtgagcgac gaggagggcg gtgaagccga caccctgctc aaccgcgccc
     1561 tgcgccatct ggccgaaagc ggcgattggg agccgcagga cgcctttacc ccctgcctgt
     1621 gccaccggct ggacgcgggc accagcggtc tggtgatgat cgccaaaacg cctttttgcc
     1681 gggatgcggt gctggaggcg atgcggctgc gccaattcaa aaagacctat ctcggcgtca
     1741 cgttcggccg gcccgcgccg cccagcggca ccctgcgcgg ctacctgagc aaggacgcgg
     1801 cccggggcct ggtgcgggtc acaagcgccc ccaggccccg cactcgcgag atcgtcaccg
     1861 gctaccgcac ccttgcggtg agcgggcgtc tcgccctgtt ggagatcgac cttgtcaccg
     1921 ggcgcaccca tcagatccgc gcccatttgg ccagcatcgg ctgcccgctg ctaggggatt
     1981 cgaaatacgg caaccaggcc gccaaccggg agctgcgcgc caaataccag cttttgaccg
     2041 catggcggct gcagctgccc caattcggtc ccggccccct gcagcagctc tccggcaggg
     2101 tgttgacggc ggagaagccc tggtattacg cgcaggtcct ggaccacacc ctaaaataaa
     2161 aagctgtagt ggggaccccg gcaaaggcag atgccctgtc ctccgggccc cggacccggc
     2221 ggcgcgatct gcgcaaaagc gcgccgtaac gggagtgtcg ggccccagcg acgccgtgca
     2281 tttttgtctt tccagggctc tacaaccccc aaagggagtc ctcaagaaag ggaatcggca
     2341 atggcgcgtt tgcgcaagaa agcttgtcct caaaaagaag atttgcgcgc agagcgtcgt
     2401 cacattcctc ccacaaccac tgaaaggctt tcgcggtttc gacctgcgaa acgcccgcgc
     2461 cagcaggccc tcagcgtgga acctctccgc tctgcgccgc ggctttccct cccaggggcg
     2521 ccgagcggtt ttgaaccggt attttcacag tgagacacag tgcaaaaaag gttggcaatc
     2581 cgcagggatc gccaaccttt ttaaatgcag ggaaatactg aaaccgtcgc ttcaaaatga
     2641 ctattttctt atctcagcgc gtcgaagcgc ttgccctttt acggctcttt ctttttctgg
     2701 tagatgagaa aatcgaattc gatctccgtc tgcagcgtgt tcagctgttc cagcttttcc
     2761 gcaccgcctc gcggcgtttt ccagtaatag ggggtcatgg cgaaaagcgc cccgatctcc
     2821 gcgccgccgg agagggcgat gcttcccgac accggcacgc ggcggcaaaa gcaaaagccg
     2881 ggatagtccg tctgccgcac cgggttttca tagggctgct catagaggac gcgtttgagc
     2941 ccgaagaggt ggcgcggccc gggcacggcg tacagcaaaa agccgcccgg ccgcagaaca
     3001 cgcaaaaatt ccgcctgggc catgggggca aaaatatcga ccaggcagtc cgccgcgccg
     3061 tccgccatgg ggatggcaaa taccccggcc accgcgaact ccaccccctt ataccgctgc
     3121 gccgccaggc gcaccgcctc ttttgagatg tcgaaagcgg cgatgcgagc cctcggcccc
     3181 agcacctgcg ccaggcggcc ggtataatac ccctctccgc agcccgcgtc cagcaccacc
     3241 ggggcctgca cctcctctaa acaggccgag gcgatgcggc ccagggcctc gctgaaaggc
     3301 gcataccagc ccgcctccag aaaggcccgc cgcgccgcca ccatgccctt gccgtccccc
     3361 gggacggcgg aatgcttttt attggcgggc agcaggttga catatccctg cgccgccctg
     3421 tcgaagctgt ggccggcggg gcagcgcagc gccctgccgc cgtcccgcag aggaccgccg
     3481 cagaccggac agcaaaacag gctctcccgc agctctttct ccccactcat ccgccgcgcc
     3541 tcccctctca atcttttgtc tttgcagtct ttttgcccgc ctcagggcaa aagctccagc
     3601 agcccggtga gctgggggca gcaggcgtcc gcccgcccgt ccggctcgct gtggaccaaa
     3661 atcgcctgca aacccgcgtt tttggccccc tcgatgtcgt cgccgacgct gtccccgatc
     3721 atgacggcgc tctgcggcag ccccgcaagc tgccgcgcgg cctcgaaaat ctcgcgcgcg
     3781 ggcttgtccc agcccacctc gcccgagacg atatggccgc tgaaataggg ggccaggccc
     3841 agcttctgcg ccacctccca cagctctgga aaattgttgg acagcaaaaa gctgcgatag
     3901 ccccgccgct gcagctccgc cagcaccggc accgcgtccg ggtagaggcg gtgccgggag
     3961 ggatcgcgca gcttggggcg cagcgccgcc acggcgcgcg ccgcggtgcg gcagtcaaag
     4021 ccgcactcct gcagcagggt gttgaagccg tcctgcacat aggcccacca agcatccccc
     4081 gtgggggtgg cgcggcccgg catcgtccac cagggcaggc aggcgtggcc cagccgccga
     4141 tgcagcgcct cctccgggca aacgtcatgg ggcagcgcct cccacagcg
//
LOCUS       DBMDYA010000178         4182 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5040_length_4182_cov_1.888508, whole genome shotgun sequence.
ACCESSION   DBMDYA010000178 DBMDYA010000000
VERSION     DBMDYA010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5040_length_4182_cov_1.888508"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            77..715
                     /locus_tag="ACZPFK_05610"
     CDS             77..715
                     /locus_tag="ACZPFK_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR1405819.1"
                     /translation="MLMVYVYVLDTLADWELGQVLAELSSGRFFKKDAPRLSLKTVGC
                     SKEPVRTMGGLTILPDLSLDDIAAEGAAMLLLPGAETWSDPKHSAVLEKAGQFLSAGA
                     AVAAICGATAALADFGLLDRRPHTSNGPGFLDTVCARYAGQKYYVDAPAAADGGLITA
                     GSAGALPWAKEIIRYLDVFEPDTLEAWYAYFSTGAPEHFFALLQSLPGADRG"
     gene            complement(943..1494)
                     /locus_tag="ACZPFK_05615"
     CDS             complement(943..1494)
                     /locus_tag="ACZPFK_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405820.1"
                     /translation="MELGKIIALNLKELRTERNLTLGQLSKLSGISKAMLSDIEKGGS
                     NPTINTIWKIANGLNVPYTKLMEGIEKEATVIRRAQTAAQSGETEHYRVYCYFRNTPV
                     RNFELFYVELDPKSANASIGHSEKAQEYLFIMQGELKLHTETSDYTLNEGDSLMFDSS
                     IGHTYINEKDVLLRFICINYYPD"
     gene            1769..2344
                     /locus_tag="ACZPFK_05620"
     CDS             1769..2344
                     /locus_tag="ACZPFK_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR1405821.1"
                     /translation="MEKRLSREEAWALLTEYTHTPALRKHALAVEATMHHFARLYGED
                     EEVWGIAGLLHDLDYEKYPREHCRKAEEIMREREIDETYIRAMNCHAYGICTDVKPES
                     QMEKTLFTIDELTGLINALCLMRPSKSVLDLEVKSVKKKFKDRSFAAGVNRDTIRSGC
                     EMLGMELDTVICETIGGMREKAQELGLKGNL"
     gene            2624..>4182
                     /locus_tag="ACZPFK_05625"
     CDS             2624..>4182
                     /locus_tag="ACZPFK_05625"
                     /inference="COORDINATES: protein motif:HMM:NF028438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HIR1405822.1"
                     /translation="MNRATIRVETLVIPTYPEPEREPLPMFAENRVHQRSSGRPYPNC
                     VTLEVDRAHRQDRAYQAVHLENDYLDVWVLPEIGGRIYAARDKSTGYDFFYRQHVIKP
                     ALIGALGSWVSGGVEFNWPYHHRASGFMPCDFTTEEAADGSVICWLSEHDPVDRMKSL
                     VGVVLPPDAAYLETRVKLCNRTPTKKSFLWWENAAVPVNESYQIFFPRDVTYVNFHYL
                     DSRISYPVAGAGVFNGIDMSEPRDISWHKNTRDATSYFACASKYDFFGGYDHGRDCGV
                     VHIGNHHISPGKKMFTWAYNQLSRSWEKALTDSDGQYAELMAGSYTDNQPNFSWLEPY
                     ETKQFSQYWYPISRIGTPDYADLDCALKLDEGHFCLQATRPLGRCTVEVRAGAEALLT
                     REVELLPCAPVFLPWTRPAGLVSVTLLRADGKRVAFYQEEEPDRLKMPPVKDPMPLAA
                     EMQSAGRLYLAGLHVEQYRDPAVMPDAYWLEALKRDPRHVPSLLGLARYSYTMARFEE
                     ARSYAERAIREL"
BASE COUNT          894 a         1232 c         1178 g          878 t
ORIGIN      
        1 cgcactgttg tcaacaatcc tatcgtacaa taaaaaagcg aacaaagcaa tcgagcgatg
       61 aaaacggagg aattttatgt taatggttta tgtttatgtc cttgacaccc tggccgactg
      121 ggagctgggg caggtccttg cggagttgag ctccggccgg ttttttaaaa aggacgcgcc
      181 gcgcctctct ctcaaaaccg tcggctgctc caaggagccc gtccgcacga tgggcgggct
      241 gaccatcctg cccgacctct ccctggacga catcgcggcg gagggggcgg ccatgctgct
      301 tttgccgggg gcggaaacct ggagcgaccc caagcacagc gccgtcctcg aaaaggcggg
      361 ccagtttctc tccgccggcg cggcggtggc cgcgatctgc ggcgccaccg ctgcgctggc
      421 cgacttcggc ctgctggacc gccgccccca caccagcaac gggcccgggt ttctggacac
      481 ggtctgcgcc cgctatgcgg gacaaaaata ttacgtcgac gcgcccgcgg cggcggacgg
      541 cggcctgatc accgcgggca gcgccggggc tttgccgtgg gcaaaagaaa tcatccgcta
      601 tctggacgtt tttgagccgg acacgctgga ggcctggtac gcctatttca gcaccggcgc
      661 gccggaacac tttttcgccc tcctgcaaag cctgcccggg gccgacaggg gctgaaccgc
      721 cgtcggaggc aaaaccggga cgctgaacac aggaacagct ctgcgggcaa aagagggtcg
      781 ggtccctcca agcaaaccgg ccacagggcg gcaaaaacgg gctgcagcgt ttttcagcac
      841 tgtttcatct cacgtttaac ccatataaat tcagtctaaa aaagcagagg agcgcttttc
      901 acgccctctg cttttcattc gatctttctc tctgccgacc ttttaatcag gataataatt
      961 gatacagata aatcgcagca gcacatcttt ttcattgata taggtatgtc cgatggaaga
     1021 atcaaacatc agtgaatccc cctcgttcag ggtgtaatcg ctggtttcgg tgtgcagttt
     1081 taattcgccc tgcatgataa aaagatactc ctgagctttt tcagagtgtc caatcgaggc
     1141 gttggcgctt ttggggtcca gctccacata gaacagttca aagttcctca ccggcgtatt
     1201 tctaaaatag caatataccc ggtaatgttc tgtttccccg ctctgcgccg ccgtctgtgc
     1261 tctgcggata acagtcgcct ctttttcgat cccctccatc agctttgtat agggcacatt
     1321 caagccattg gcaatcttcc agatggtgtt gatggtcggg ttgctgccgc ctttttcaat
     1381 gtccgacaac atggctttgc tgatcccgga caactttgat aactgcccca gcgttaaatt
     1441 tcgttcagta cgcaattctt ttaaattgag tgcgatgatt tttccaagtt ccatagttgc
     1501 cctctttttg cttgacaaat aaattgaaca attgtaaaat atatcaaaca cttcgttcat
     1561 tgtgtcgtac gaatatcgat tatactgtaa acagtataaa aaaaaacgct tcaaatagca
     1621 agcgaggggg gctgccggcg tcgacgccgt tgttttgctg gttgcctcaa aagctgattt
     1681 tcgatcaatg cccttttcaa aaaatgatat cctgctgccg cgcagaacgg tagttatgat
     1741 aataaaattt gcaggaggtc ctcaaaaaat ggaaaaaagg ttgtcaaggg aagaggcgtg
     1801 ggcgctgttg acggaatata cccacacccc cgcgctgcga aagcacgcgc tggcggtcga
     1861 ggcaacgatg caccactttg cccggctgta tggggaagac gaagaggtct ggggcattgc
     1921 cggtttgctg catgacctgg actacgaaaa atacccgcgg gaacattgca gaaaagcgga
     1981 ggaaatcatg cgtgagcgcg aaattgatga aacatacatc cgcgccatga actgtcatgc
     2041 ctacggtatc tgtaccgacg tgaagccgga aagccagatg gaaaaaacgc tgttcacaat
     2101 cgacgagctc acaggtctga tcaatgcctt gtgcctgatg cgcccctcta aaagtgtact
     2161 tgatttggaa gtgaaatccg tcaaaaagaa attcaaggat cggtcgtttg cagccggcgt
     2221 caaccgagat acgatccgca gcggctgtga aatgctgggg atggagttgg atacggtgat
     2281 ttgcgaaacg atcgggggca tgagggaaaa ggcgcaggaa ctaggattga aagggaacct
     2341 ataatttcca gattagaaga atatgcaaaa aagggagagc ggagggcgat tatttgcccc
     2401 ccgttttttg gattagctca ccctgccgcc agtgcccaga cggttcgcgc cccttttctc
     2461 ccgcggatgc aagtccaact tatgtccggc gcgcagggcg gcatccccgt ctaaaacagc
     2521 tgtttttttt cacgcttttt ttgtctttcc ctcccggccc aaatccttta tgatgagagc
     2581 aacagaccgg ccccggcacg gggccgcaaa ggtggttgac aagatgaaca gagcgacgat
     2641 ccgggtggag accctcgtga tccccaccta ccctgagccg gaacgggagc ccctgcccat
     2701 gttcgcggaa aaccgcgtcc atcagcgctc ctccggcagg ccctacccca actgcgtcac
     2761 gctggaggtg gaccgcgccc accggcagga ccgggcctat caggccgtac acctggaaaa
     2821 cgattatctg gacgtctggg tgctgcccga gatcggcggg cgcatctatg cggcgcggga
     2881 caaatccacc gggtacgact ttttttaccg ccagcacgtg atcaagcccg ccctcatcgg
     2941 ggcgctgggc tcctgggtgt cggggggcgt cgagttcaac tggccctatc atcaccgcgc
     3001 ctccggcttt atgccctgcg acttcaccac cgaggaggcc gcggacggct cggtgatctg
     3061 ctggctctcc gagcacgacc cggtggaccg gatgaagagc ctggttggcg tcgttctgcc
     3121 ccccgacgcc gcctatctgg agacccgggt gaagctgtgc aaccgcaccc ccaccaaaaa
     3181 gtcttttctc tggtgggaaa atgccgccgt gccggtgaac gagagctatc agatcttttt
     3241 cccccgcgac gtgacctatg taaattttca ctatcttgac tcccgcatct cctacccggt
     3301 ggcgggggcg ggcgtcttca acggcatcga catgtccgag ccgcgggaca tctcctggca
     3361 caagaacacc cgggacgcca cctcctactt tgcctgtgcc tccaagtacg atttcttcgg
     3421 cggctacgac cacggccggg actgcggcgt ggtgcacatc ggcaatcacc atatctcgcc
     3481 gggcaaaaag atgtttacct gggcctataa ccaactctcc cgctcctggg aaaaagccct
     3541 caccgacagc gacggccagt acgccgagct gatggccggc tcttacaccg acaaccagcc
     3601 caacttttcc tggctggagc cctatgagac aaagcagttc tctcaatatt ggtaccccat
     3661 cagccgcatc ggcacgccgg actatgccga cctggactgc gcgctgaagc tggacgaggg
     3721 gcatttctgc ctgcaggcca cccgcccgct ggggcgctgc accgtcgagg tgcgggcggg
     3781 ggcggaggcc ctgctcaccc gggaggtgga gcttctgccc tgcgcgccgg tctttctgcc
     3841 ctggacacgg cccgcgggcc tggtgtcggt caccctcctg cgcgcggacg gcaagcgcgt
     3901 cgccttttat caggaggagg agccggaccg cctgaagatg ccgccggtca aggacccgat
     3961 gccgctggcc gcggagatgc agtccgccgg gcggctgtac ctcgccggcc tgcacgtgga
     4021 gcagtaccgc gacccggcgg tcatgccgga cgcctactgg ctggaggccc tcaagcgcga
     4081 cccccgccat gtgccctcgc tgctgggcct ggccaggtac agctatacca tggcccgctt
     4141 tgaggaggcc cggagctatg ccgagcgggc gatccgcgag ct
//
LOCUS       DBMDYA010000179         4178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5049_length_4178_cov_1.510179, whole genome shotgun sequence.
ACCESSION   DBMDYA010000179 DBMDYA010000000
VERSION     DBMDYA010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_4178_cov_1.510179"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="ACZPFK_05630"
     CDS             complement(<1..431)
                     /locus_tag="ACZPFK_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1405823.1"
                     /translation="MYYNKVEICGVNTAKLPVLKEQEKIELIKKAQQGDQEARQKMIA
                     GNLRLVLSVVQKFTGRKENMDDLFQVGCIGLIKAVDGFDTSLGVRFSTYGVPMIVGEI
                     RRFLRDNNSVRVSRSVRDLAYKAMQAREELQKRYGREPTME"
     gene            complement(587..1222)
                     /gene="sigE"
                     /locus_tag="ACZPFK_05635"
     CDS             complement(587..1222)
                     /gene="sigE"
                     /locus_tag="ACZPFK_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR1405824.1"
                     /translation="MNGPETLPPPLTPAEEKEVFAALSRGEQSARDTLITHNLRLVVY
                     IAKKFENTGISIEDIISIGTIGLIKSVNTFAPEKNIKLATYASRCIENEILMYLRKSS
                     NRRVETSIDEPLNTDGEGNELLLGDVLGTDPDIVGAEIEQDDEKTMLYRAVNRLSDRE
                     RLIMQMRFGLLDGTERTQKEVADMIGISQSYISRLEKRIIKRLRQDLERAM"
     assembly_gap    1242..1251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1495..2370)
                     /locus_tag="ACZPFK_05640"
     CDS             complement(1495..2370)
                     /locus_tag="ACZPFK_05640"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1405825.1"
                     /translation="MRSVVYVDMLVAVNFILDFFLLLGAKLLSGSVTSRWRCLLGAGL
                     ASLFSLVLLLPPLPLALQLLIKVAGSVLPVLAAFRYTGPRQMVKAAVWFLLLNFFLAG
                     AVFFVMYTFSAPGIEQNNLALYFNISPVILIMSVLAVYLALQLISLLLGRPRPEQPVQ
                     LELWLDGERIAASALLDTGFSLRDVLSGCPCLLLSYPEVKGQLSPRLREALERYYSGG
                     VPEVEKGPPVRMIPCRTVAGESLLPAFCCEKCVLRYRGQTVELPGVPAAFTRHPLADG
                     RSAGLVGPELLQPIL"
     gene            complement(2762..>4178)
                     /locus_tag="ACZPFK_05645"
     CDS             complement(2762..>4178)
                     /locus_tag="ACZPFK_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1405826.1"
                     /translation="SIGRDYDTMIITGPNTGGKTVSLKTAGLLLAMASCGFLIPASER
                     SQVCLFRDILVDIGDEQSIEQSLSTFSGHITNIVEILKEAGPDTLVLLDELGAGTDPA
                     EGASLAVSVIETLREKGSRIMATTHYGELKVFALETEGVQNAGCEFDLETLRPTYRLS
                     VGVPGRSNAFVIGEKLGLPAPVIDRAKRHLSAEDKRFETVLGQLEDLKLEIKEDKAEI
                     ERLRYVAENQLDSAREERDKLIRQGEEELAAARRKAKELEQKVQDTAYGLMDEMKALQ
                     KKENLSAAQKLQRARQIARKEALGITAGLGSEPEVKNYVPLTSVKVGQVVVMADIGRN
                     ATVKNLPDKNGMVEVLCGAIKTKVPLSSLMAAAEPKNKKAPGPKYRSSGANASVTRTP
                     AMEINLIGLTAEEAVMEAERFIDSAVLSGMTQVYLIHGKGAGILRKALHAMLKTNKSV
                     KSYRLGNYGEGEDGVSIVSLA"
BASE COUNT          744 a         1350 c         1262 g          812 t
ORIGIN      
        1 tcctccatgg tcggctcgcg gccgtacctc ttttgcagct cttcccgcgc ctgcatggcc
       61 ttgtaggcga ggtcgcgcac cgagcggctg acccgcaccg aattgttgtc ccgcaaaaag
      121 cgcctgatct cgcccacgat catcggcacc ccgtaggtgg aaaaccgcac ccccagcgag
      181 gtgtcgaagc cgtccaccgc cttgatcagg ccgatgcagc cgacctggaa caggtcgtcc
      241 atgttctctt tgcggccggt aaatttctgc actacgctga gcaccagccg caggttgccg
      301 gcgatcatct tctgccgggc ctcctgatct ccctgttggg cttttttaat gagttcgatc
      361 ttttcctgtt ctttcaaaac cggcagcttt gccgtattca cgccgcagat ctcgaccttg
      421 ttgtaataca aaagctcccg cactccattc catcgaggtt acgatggtta gtatgggtgc
      481 gggaggtgcc tttaatctgt tttgcggggc tggaaaaaga tgcatcccaa cgctgcaaag
      541 cgcattggct ttacagattt gaaaaatcat tttttgtccc aggcggtcac atcgcccgct
      601 ccaggtcctg ccgcaggcgt ttgatgatgc gtttttccag ccgggagata tagctctgcg
      661 agatgccgat catgtcggcc acctctttct gggtgcgctc ggtgccgtcc aaaagcccga
      721 accgcatctg catgatcagg cgttcccggt ccgacaggcg gttcaccgcc cggtagagca
      781 tggtcttttc atcgtcctgc tcgatctcgg cccccacgat atccgggtcg gtgcccagca
      841 catcgcccag cagcagctcg ttgccctccc cgtcggtgtt gagcggctcg tcgatgctgg
      901 tctccacccg ccggttgctg gatttgcgca ggtacatgag gatctcgttt tcaatgcagc
      961 gggaggcgta ggtggccagc ttgatgttct tttccggcgc aaaggtattg actgatttga
     1021 tcaggccgat ggtaccgatg gagatgatgt cctcgatgga gatgccggta ttctcaaact
     1081 ttttggcgat gtagaccacc aggcgcaggt tatgggtgat aagggtgtcc cgcgccgact
     1141 gctccccgcg ggaaagggcg gcaaaaacct ctttttcctc cgccggggtg aggggcgggg
     1201 gcaaggtctc cgggccgttt atgtaatagg cgtagtccgg annnnnnnnn ntccggaaag
     1261 cggagccgga ggagcagctg ggtaaaaaag cggcggagcg cgccgggcga caggcagttc
     1321 atcttcatca tccattccgc cgcttcgcat cggcacaaat taagtaaaac agcgcgcatc
     1381 tcgtcacgcg aagcagggag acgacaagat gatcctgcgc agggggcgtg tgcggccgcg
     1441 cggcaaagcg cccggtgaga ggcgggcgcc ttgctctttt gcgcggttcc cgcctcacag
     1501 gatcggctgc agcagttcgg ggccgaccag gcccgcactg cgtccgtccg ccagggggtg
     1561 gcgcgtgaaa gcggccggta ccccgggcag ctccaccgtc tgtccccggt accgcagcac
     1621 gcatttttca cagcagaatg cgggcagcag gctctccccc gccacggtcc ggcaggggat
     1681 catccgcacc ggcgggcctt tttccacctc cggcaccccg ccgctgtaat agcgctcgag
     1741 ggcttcgcgc agccgcgggg agagctggcc tttcacctcc ggatagctga gcagcaggca
     1801 gggacagccc gacagcacat cccgcaggga aaaaccggtg tccaaaagag cggaggcggc
     1861 gatgcgctcc ccgtccagcc acagctccaa ctgcaccggc tgctccggcc gcggcctgcc
     1921 cagcagcagc gagatcagct gcagcgccag atacacggcc agcaccgaca tgatgaggat
     1981 caccggggaa atattgaagt agagggccag gttgttctgc tcaatgcccg gcgcggaaaa
     2041 ggtgtacatc acaaagaaca ccgcgcccgc gagaaagaag ttgagcagca aaaaccagac
     2101 ggcggccttg accatctggc gcggccccgt atagcggaaa gccgccagca ccggcagcac
     2161 gctgccggcg accttgatga gcagctggag ggcgagcggc aggggcggca gcagcagcac
     2221 cagcgagaaa aggctggcga gcccggcccc cagcaggcag cgccaacggc tggtgacact
     2281 gcctgagagc agctttgccc ccaacagcag aaagaaatcg aggatgaagt tgaccgccac
     2341 cagcatatcc acatacacca cgctgcgcat cgcctctcac ccccctcatg cctttcagtc
     2401 tacgccccgg gccccgcaaa gggtgtcgaa gcgaggacgg gcagggcggc gcttttccgc
     2461 ttcggcccgc tgcccggcaa agattttttt ggagaccggg cattcggtcc gggcctgccg
     2521 tcccgccgga aagcgggcgt tccctccccg ccgccctcgc cgccgggcgg atgccgcccc
     2581 cggggccgtg ctttcaaaag cggggaggcg gaaagcccgt ctatctttct gtggggcaag
     2641 gcgctgaaag gcagccgggc aaaagcaaag cgcaggcccc ggcactctgc aatgccgggg
     2701 cctgcgccct ccaaaaaaga tctgctttta taacgaagat agggaaacag ggggaaagcc
     2761 ctcaggcaag tgagacaatt gaaaccccgt cttccccttc cccatagttg cccaggcgat
     2821 agcttttgac cgacttgttg gtcttcagca tggcgtgcag cgccttgcgc aggataccag
     2881 cgcccttgcc gtggatcaga tagacctggg tcatgccgct gagcaccgcg ctgtcgatga
     2941 agcgctcggc ctccatcacc gcctcctcgg ccgtcaggcc gatgaggttg atctccatgg
     3001 ccggggtgcg ggtgacgctg gcgttggcgc cgctgctgcg gtatttgggg cccggggcct
     3061 ttttgttttt cggctccgcg gcggccatca gcgaggacaa cggcaccttg gtcttgatgg
     3121 cgccgcagag cacctccacc atgccgtttt tgtcgggcag atttttcacg gtggcgttgc
     3181 ggccgatatc cgccatcacc accacctgcc ccaccttgac gctggtgaga ggcacatagt
     3241 ttttcacctc cggctcactg cccaggcccg cggtgatgcc cagcgcctcc ttgcgggcga
     3301 tctggcgcgc ccgctgcagc ttctgcgcgg cggagagatt ttcttttttc tgcagggctt
     3361 tcatctcatc catcaggccg taggcggtgt cctgcacttt ctgttccagc tctttggcct
     3421 tgcgccgcgc tgcggccagc tcctcctcgc cctgccggat gagcttatcc cgctcttccc
     3481 gggcggagtc gagctggttt tccgccacat atctcagccg ctcgatctcc gccttgtcct
     3541 ccttgatctc cagcttgaga tcctccagct ggcccagcac cgtctcaaag cgcttgtcct
     3601 cggcggacag atgccgcttt gcccggtcga tcaccggggc cggcaggccc agcttttcac
     3661 cgatcacgaa agcgttggag cgcccaggca ccccgacgct gaggcggtag gtggggcgca
     3721 gggtctcaag gtcaaactcg cagccggcgt tctgcacccc ctcggtctcc agcgcaaaga
     3781 ctttcagctc cccgtaatgg gtggtggcca tgatgcggct gcctttctcc cgcagcgtct
     3841 cgatgaccga caccgccagg gaggcgccct cggccgggtc ggtgcccgcg cccagctcgt
     3901 ccagcagcac cagcgtgtcc ggccccgcct ctttcaatat ctccacgata ttggtgatat
     3961 gccccgaaaa ggtggaaagg ctctgctcaa tgctctgctc atcgccgatg tccaccagga
     4021 tatcccggaa caggcagacc tgactgcgct cggaggccgg gatcaaaaag ccgcaggagg
     4081 ccatggccag cagcaggccc gccgttttca ggctgaccgt cttgccgccg gtgttggggc
     4141 cggtgatgat catggtgtca tagtccctgc cgatggag
//
LOCUS       DBMDYA010000180         4174 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5056_length_4174_cov_1.580162, whole genome shotgun sequence.
ACCESSION   DBMDYA010000180 DBMDYA010000000
VERSION     DBMDYA010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5056_length_4174_cov_1.580162"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="rplC"
                     /locus_tag="ACZPFK_05650"
     CDS             <1..585
                     /gene="rplC"
                     /locus_tag="ACZPFK_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR1405827.1"
                     /translation="DENGKVVPVTIIEAGPCVVVQKKTVESDGYQAVQLGYGDVSAKR
                     VTKPAKGHFDKADVAPKKTLREFRLDDISGLAVGDIIKADAFAAGDHIDVVGISKGKG
                     YAGTIKRWGGHRLRESHGTGPVARHAGSNGSVSSPSRVFKGKKLPGHLGAERVTIQNL
                     TVVKIDAENNLIAIKGAVPGAKGSVVTIHDSVKA"
     gene            601..1224
                     /gene="rplD"
                     /locus_tag="ACZPFK_05655"
     CDS             601..1224
                     /gene="rplD"
                     /locus_tag="ACZPFK_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR1405828.1"
                     /translation="MAKFDVLDMSGKKVSTIELSDAIFGIQPNERVMHAAVVNFLANQ
                     RQGTQSTKTRSEVSGGGRKPWRQKGTGRARQGSTRSPQWTHGGVALGPKPRDYSYTLN
                     KKVKRLAMFSALSSKAASGDVIVVEKFDCPEFKTKTMVNMLNAVGATGKSLVVAPEVD
                     KKVVKSAANIPGVKTTLATALNVYDILNAGKFVISVDAAKKLEEVFA"
     gene            1224..1520
                     /gene="rplW"
                     /locus_tag="ACZPFK_05660"
     CDS             1224..1520
                     /gene="rplW"
                     /locus_tag="ACZPFK_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR1405829.1"
                     /translation="MKVAAQDIILAPVITENSMAGMQNRKYTFKVATSATKVDVARAC
                     EELFGVKVAKVNTINVRGRYRRQGMHAGYTAASKKAIVTLKPDSKTIAFFDSML"
     gene            1655..2485
                     /gene="rplB"
                     /locus_tag="ACZPFK_05665"
     CDS             1655..2485
                     /gene="rplB"
                     /locus_tag="ACZPFK_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1405830.1"
                     /translation="MAIKKYKPTTPGRRGMTVTDYSVLSKVEPERSLLEPMKHNSGRN
                     NTGRITVRHHGGGNRTKYRVVDFKRNKFDVPATVKTLEYDPNRSAHIALIQYEDGVKS
                     YIIAAEGLKVGDTVMNGPNADIKPGNCLPFSAIPVGTVIHNIELYPGKGAQLVRSAGN
                     MAQLMAKENGYALVRLPSGEMRNVPLNCVASIGQVGNIDHENVNYGKAGRKRHMGWRP
                     AVRGSVMNPCDHPHGGGEGKSPVGRPSPVTPWGKPTMGYKTRKHHNRSDKFIVKRANG
                     "
     gene            2503..2775
                     /gene="rpsS"
                     /locus_tag="ACZPFK_05670"
     CDS             2503..2775
                     /gene="rpsS"
                     /locus_tag="ACZPFK_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1405831.1"
                     /translation="MGRSVKKGPFVQPALLKRVKEMNAAGEKRVLKTWSRSSTIFPQF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHAGAKTTR"
     gene            2792..3130
                     /gene="rplV"
                     /locus_tag="ACZPFK_05675"
     CDS             2792..3130
                     /gene="rplV"
                     /locus_tag="ACZPFK_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1405832.1"
                     /translation="MEARAKLSYARIAPRKVSIVLDLIRNQPLDKALAILQYTPKAAS
                     ELLYKLVKSAAANAENNFNMDKNSLYVAECYVTPGPTLKRVRPRAQGRAYRILKRTSH
                     ITVVLKEMEG"
     gene            3146..3808
                     /gene="rpsC"
                     /locus_tag="ACZPFK_05680"
     CDS             3146..3808
                     /gene="rpsC"
                     /locus_tag="ACZPFK_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1405833.1"
                     /translation="MGQKVNPHGFRVGVIKDWDSRWFTGKKEFGDTLVEDHKIRTVLK
                     KQLYAAGVPRIEIERDAQKVKVNIHCSKPGMVIGRGGSEIDKLRVQIEKMIGKPVVVN
                     IVEVRTPDLNAQLVAENVASQLERRIAFRRAMKQCIGRTMRMGAKGIKISVGGRLGGA
                     DIARTEHYHEGTIPLQTLRADIDYGFTEANTTYGKIGVKVWIYKGEVLKGAKPRKEGG
                     SK"
     gene            3808..>4174
                     /gene="rplP"
                     /locus_tag="ACZPFK_05685"
     CDS             3808..>4174
                     /gene="rplP"
                     /locus_tag="ACZPFK_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1405834.1"
                     /translation="MLLPKRVKYRRQQRGRMKGKAMRGNKVNQGEYGLQALEPAWITS
                     NQIEAARIALTRYIKRGGQVWIKIFPDKPVTEKPAETRMGSGKGSPEYWVAVVKPGRV
                     LFEIAGVSEENAREAMRLAA"
BASE COUNT          943 a         1334 c         1263 g          634 t
ORIGIN      
        1 gatgaaaacg gcaaggtcgt gccggtcacc atcatcgagg cgggcccctg tgtcgtggtg
       61 cagaagaaga ccgtcgagag cgacggctat caggctgtgc agctgggcta cggcgacgtt
      121 tccgccaaaa gagtcaccaa gcccgccaag ggccactttg acaaggcgga cgtcgccccc
      181 aagaagaccc tgcgcgagtt ccgtcttgac gacatctccg gccttgcggt cggcgacatc
      241 atcaaggccg acgcctttgc ggcgggcgac cacatcgacg tggtcggcat cagcaagggc
      301 aagggctacg cgggcaccat caagcggtgg ggcggccacc ggctgcgcga gagccacggc
      361 accggcccgg tcgcgcgcca cgccggttcc aacggttccg tcagctcccc ctcccgtgtg
      421 ttcaagggca aaaagctgcc cggccacctg ggcgcggagc gggtcaccat ccagaacctg
      481 accgttgtca agatcgatgc ggaaaataat cttattgcta tcaagggtgc ggtccccggt
      541 gccaagggca gcgtggtcac catccatgac agcgttaagg cgtaaaggag ggaaagcaaa
      601 atggcgaaat ttgatgtttt ggatatgtcc ggcaagaaag tttccacgat cgagctttcc
      661 gacgccatct ttggtatcca gcccaacgag cgggtcatgc acgcggctgt ggtgaatttc
      721 ctggccaacc agcgccaggg cacccagagc accaagaccc gcagcgaggt ttccggcggc
      781 ggccgcaagc cgtggcgcca gaagggcacc ggccgcgccc gtcagggctc gacccgctcc
      841 ccgcagtgga cccacggcgg cgtcgcgctc ggccccaagc cccgcgatta cagctacacc
      901 ctgaacaaaa aggtcaagcg ccttgcgatg ttcagcgccc tgtcgtccaa ggcggccagc
      961 ggcgacgtga tcgtggtgga aaagtttgac tgccccgagt tcaagaccaa gaccatggtc
     1021 aacatgctga acgcggtcgg cgccaccggc aagagcctgg tcgtcgcgcc cgaggtcgac
     1081 aagaaggtcg tcaagagcgc cgccaacatc cccggcgtca agaccaccct tgccaccgcg
     1141 ctgaatgtct atgacatcct gaacgcgggc aagttcgtca tcagcgtaga tgccgcgaaa
     1201 aagcttgagg aggtatttgc gtaatgaagg ttgcagcaca ggatatcatc cttgccccgg
     1261 tcatcaccga gaattcgatg gccggtatgc agaacaggaa gtacaccttt aaagtggcca
     1321 ccagcgccac caaggtcgac gtcgcgcggg cctgcgagga gctgttcggc gtgaaggtcg
     1381 ccaaggtcaa caccatcaac gtgcgcggcc gttatcgccg ccagggcatg cacgccggct
     1441 acaccgccgc ctccaaaaag gcgatcgtca ccctgaagcc ggactcgaag accatcgcgt
     1501 tcttcgacag catgctgtaa agagggcggg cgctttgccc ggcagcctct tccccgccgg
     1561 accggccttt tcttccggcg cgggcgtgat cccgggaggg gtcagctatg gagatatgac
     1621 tccgataaga atttcataag aaagagagat aacaatggcc attaaaaagt acaaaccgac
     1681 tactccgggc cgccgtggta tgacggtcac cgattactcg gtcctttcca aggtcgagcc
     1741 cgagcggagc ctgcttgagc cgatgaagca caacagcggc cgcaacaaca ccggccgcat
     1801 caccgtccgc catcacggcg gcggcaaccg caccaaatac cgcgtggtcg acttcaagcg
     1861 caacaagttc gacgtccccg cgacggtcaa gaccctcgag tacgatccca accgctcggc
     1921 ccacatcgcc ctgatccagt atgaggacgg cgtgaagagc tacatcatcg cggccgaggg
     1981 cctcaaggtg ggcgacacgg tcatgaacgg ccccaacgcc gacatcaagc cgggcaactg
     2041 cctgcctttc tcggccatcc ccgtgggcac cgtcatccac aacatcgagc tgtaccccgg
     2101 caagggcgcg cagctggtgc gctcggcggg caacatggcc cagctgatgg cgaaagagaa
     2161 cggctacgcg ctggtgcgcc tgccctccgg cgagatgcgc aacgtgccgc tgaactgcgt
     2221 ggccagcatc ggccaggtcg gcaacatcga ccatgagaac gtcaactacg gcaaggccgg
     2281 ccgcaagcgt cacatgggct ggcgtcccgc cgtccgcggc agcgtcatga acccctgcga
     2341 ccacccgcac ggcggcggcg agggcaagag ccccgtgggc cgtccgagcc cggtcacccc
     2401 gtggggcaag cccaccatgg gttacaagac ccgcaaacat cacaaccgtt ctgacaagtt
     2461 tattgtcaaa cgcgccaacg gttaaggaga ggagagagca acatgggtag aagtgttaaa
     2521 aagggacctt tcgtacagcc cgcactgctg aagcgcgtca aagagatgaa cgccgccggt
     2581 gagaagcgcg tgctcaagac ctggagccgc tcctctacga tcttcccgca gttcgtcggc
     2641 cacacctttg cggtgcacga cggccgcaag catgtgcccg tctacgtgac ggaggatatg
     2701 gtgggccaca agctgggcga gttcgccccc acccgcactt tcaagggcca cgcgggcgcc
     2761 aaaactacga ggtaagagga ggattcacaa aatggaagca agggcaaaac tgagctatgc
     2821 ccgcattgcg ccgcgcaaag tctcgatcgt gctggacctg atccgcaacc agccgctgga
     2881 caaggcgctc gccattctgc agtatacacc gaaggccgcc tctgagctcc tttacaaact
     2941 ggtgaaatct gccgcggcga atgcggagaa caatttcaac atggacaaga acagcctgta
     3001 tgtggcagag tgctacgtta cccccggccc caccctgaag agggtccggc cccgcgcaca
     3061 gggccgcgcg tacagaatac tcaagaggac cagccacatc accgtggttc tcaaagagat
     3121 ggaagggtaa ggagggtaac agagtatggg tcagaaagtc aatccgcacg gtttccgcgt
     3181 cggcgtcatc aaggattggg acagccgctg gttcaccggc aaaaaagaat tcggcgacac
     3241 cctcgttgag gaccacaaga tccgcaccgt gctgaaaaag cagctctatg ctgccggcgt
     3301 cccccgcatc gagatcgagc gcgacgccca gaaggtcaag gtcaacatcc actgctcgaa
     3361 gcccggcatg gtcatcggcc gcggcggcag tgaaatcgat aagctccgcg tccagatcga
     3421 gaagatgatc ggcaagccgg tcgtggtcaa catcgtcgag gtccgcaccc cggacctgaa
     3481 cgcgcagctc gtggcggaaa acgtcgcctc ccagctggag cgccgcatcg ctttccgccg
     3541 cgcgatgaag cagtgcatcg gccgcaccat gcgcatgggc gcaaagggca tcaagatcag
     3601 cgtcggcggc cgcctgggcg gcgcggacat cgcccgcacc gagcactatc acgagggcac
     3661 catccccctg cagaccctgc gcgccgacat cgactacggc ttcaccgagg cgaacaccac
     3721 ctacggcaag atcggcgtca aggtctggat ctacaagggc gaagtgctca agggcgcaaa
     3781 acctcgcaaa gaaggaggca gcaagtaatg cttttaccca agcgtgtcaa ataccgccgt
     3841 cagcagcgcg gccgcatgaa aggcaaagca atgcgcggca acaaggtcaa ccagggcgag
     3901 tatggcctgc aggctctcga accggcgtgg atcacctcca accagatcga ggccgcccgt
     3961 atcgcgctga cccgctacat caagcgtggc ggccaggtgt ggatcaagat tttcccggat
     4021 aagccggtca ccgaaaagcc cgccgagacc cgcatgggct ccggtaaagg cagccccgag
     4081 tactgggtgg ccgtggtcaa acccggccgc gtgctgttcg agattgccgg cgtctctgag
     4141 gagaacgccc gcgaggccat gcgtctcgcc gcgc
//
LOCUS       DBMDYA010000181         4170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5060_length_4170_cov_1.726960, whole genome shotgun sequence.
ACCESSION   DBMDYA010000181 DBMDYA010000000
VERSION     DBMDYA010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_4170_cov_1.726960"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="ACZPFK_05690"
     CDS             <1..480
                     /locus_tag="ACZPFK_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HIR1405835.1"
                     /translation="TVTDLTEDCGVNRQTFYYHFRDIYDLVEWIYTSEASAALGEKKT
                     YDTWQQGFLEIFRYAQENRAFVLRTYHSLSREHLERYLFSETYALLEGVVEEQAAGRS
                     IRSEDKAFIANFYKYGFVGLMLDWIGAGMKEDPHALVGRLELVIHGNIAAALGRFTH"
     gene            880..2655
                     /locus_tag="ACZPFK_05695"
     CDS             880..2655
                     /locus_tag="ACZPFK_05695"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1405836.1"
                     /translation="MYYSNGNYEAFARPEKPKDVDKKSAYLVGSGLASLAAACFLVRD
                     GQMKGERIHILEELALPGGSCDGVQDPTRGFVMRGGREMENHFECLWDLFRSIPSIET
                     EGVSVLDEYYRLNKHDPNYSLCRATEQRGRDAHTDGKFTLSDKAAMEIMKLFFTPDEQ
                     LYDKTLDDVFDEEFYSSNFWLYWQTMFAFERWHSALEMKLYIQRYIHHIGGLPDFSAL
                     KFTKYNQYESLILPMVKYLQAHGVRFEYGVRVTNVRFEITDGKKTARQIVCVRNGSEE
                     TIDLIEDDLVFVTNGCCTESSAYGDQDHAPVVTVKNGEGACWDLWRNIAAQDPSFGHP
                     EKFCSDIGRSNWESATVTTLDGRIPPYLEKICKRDPFSGRVVTGGIVTVKDSGWYMSW
                     TINRQPQFHSQGKDQLCVWVYGLYTDRPGDFVKKPMKECTGMEITEEWLYHLGVPEAE
                     IPDMAAHSAVCVPVMMPYITAFFMPRAAGDRPAVVPEGCVNFAFLGQFAETPRDTIFT
                     TEYSVRTAMEAVYTLLDVDRGVPEVWGSVYDVRALLDATVKMMDGKKLTDVKLPFAAQ
                     LVEKKALKAVEGTVVEELLRRYHAI"
     gene            complement(2925..3185)
                     /locus_tag="ACZPFK_05700"
     CDS             complement(2925..3185)
                     /locus_tag="ACZPFK_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR1405837.1"
                     /translation="MVEKLSDLPNIGPELERQLARAGVDTPEQLRALGSREAWLRIQA
                     DDPSACLHRLSALEGAVRGVKKAQLPPEVKAELKAFYRTHKG"
     gene            complement(3472..>4170)
                     /locus_tag="ACZPFK_05705"
     CDS             complement(3472..>4170)
                     /locus_tag="ACZPFK_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1405838.1"
                     /translation="LNADPRYLQRVVPALAELTGLPLRRADDLIDATQNLDCFVEVSG
                     LLKTCAVDLSKLANDLRLLSMGPRAGISEIALPARQNGSSIMPGKVNPVLPEVVNQVA
                     FRIMGNDATITLAAEAGQLELNAFEPVLLYSLFESAELLRNAVRTFVDHCVTGIRAER
                     ENCRRMVERSAGVAAALCPLIGYERSAALAKEAVREEVPVRLLAEREGVLPAEQLQEV
                     LEPLHMTGPARCGA"
BASE COUNT          781 a         1340 c         1240 g          809 t
ORIGIN      
        1 accgtcaccg acctcaccga ggactgcggt gtcaaccgac agacctttta ctatcatttc
       61 cgcgacatct acgacctggt ggaatggatc tataccagcg aggccagcgc tgcgctgggc
      121 gaaaaaaaga cctatgacac ctggcagcag ggctttttgg aaatctttcg ctacgcgcag
      181 gaaaaccgcg cctttgttct gcgcacctat cactccctga gccgggagca cctggagcgc
      241 tatctgttca gcgagaccta cgccctgctg gagggggtgg tggaggagca ggccgccggg
      301 cgttcgatcc gcagcgagga caaagctttc atcgcaaact tttacaaata cggctttgtg
      361 ggcctgatgc tcgactggat cggagccggc atgaaagagg acccccacgc cctggtgggg
      421 cggctggagc tggtgatcca cggcaatatc gccgctgcgc tgggccgctt cacacactga
      481 gcgtccggcc gtcgcgtcgc ctcccttttc ccggcgttat cgttcccgcc cacgttttgc
      541 cgtccatttt tttagacaaa acccttttcc tgaccaaatt ttatacagct ctgccgccgt
      601 gccgaaaatc ccgacacggc tttttttctg tccatggcgc cgccttgcac ggcgcgctat
      661 catagagtca tcgaaaggaa aggcggcgca cggaggacga tagaccccgc cgtaccactt
      721 ttgagaggcg ccgttcaagc tcccggcatc gtttcctccc gggggctccg ggcgcccggc
      781 cgatcttttg acggctcttt ctcgccgcgg tcgcggcggc tgcagcggat ggctcaaatc
      841 agcgcctctg ctgctattct ttagacggag ggatgacaaa tgtattattc aaacggaaac
      901 tatgaggcgt tcgcacgccc cgaaaagccg aaagacgtgg acaaaaaatc cgcttatctg
      961 gtgggctccg ggctggcctc gctggccgcc gcctgctttt tggtgcggga cggccagatg
     1021 aaaggcgaac gcatccacat cctggaagag ctcgctttgc ccggcggctc ctgcgacggc
     1081 gtgcaggacc ccacccgcgg ctttgtgatg cgcggcggcc gcgagatgga aaaccatttt
     1141 gaatgcctgt gggacctttt ccgctccatt ccctccatcg agacagaggg cgtctcggtg
     1201 ctggacgaat actatcggct gaacaagcac gaccccaact actccctctg ccgcgccacc
     1261 gaacagcgcg gccgggacgc ccacaccgac ggcaagttca ctttgtccga caaggcggca
     1321 atggagatca tgaagctgtt tttcaccccg gacgagcaat tatacgacaa aacgctggac
     1381 gatgtgttcg acgaggaatt ctactcctcc aacttctggc tctactggca gacgatgttc
     1441 gcttttgagc gctggcactc ggcactggag atgaagctct acatccagcg ctatatccat
     1501 cacatcggcg gcctgcccga tttttccgct ctcaaattca ccaaatacaa ccagtacgaa
     1561 tccttgattt tgccgatggt gaagtatctg caggcgcacg gcgtgcggtt tgaatacggc
     1621 gtgcgggtca ccaacgtgcg ctttgagatc acagacggca aaaagaccgc ccggcagatc
     1681 gtctgtgtgc ggaacggcag cgaggagacc attgacctga tcgaggacga tctggtcttc
     1741 gtcaccaacg gctgttgcac cgagagctcc gcctacggtg accaggatca cgcgccggtg
     1801 gtcaccgtga aaaacggcga gggagcctgc tgggacctgt ggcgcaatat cgccgcccag
     1861 gacccgtcct tcggtcatcc tgaaaaattc tgctccgaca tcggtcgcag caactgggaa
     1921 tcggccaccg tcaccacgct ggacggcagg ataccgcctt atctggaaaa gatctgcaag
     1981 cgcgacccgt tctccggccg agtggtcacc ggcggcattg tgaccgtcaa ggactccggc
     2041 tggtacatga gctggaccat caaccgccag cctcagttcc acagccaggg caaggatcag
     2101 ctctgcgtct gggtgtacgg cctgtacacc gaccgccccg gcgattttgt caaaaagccg
     2161 atgaaagagt gcaccggcat ggagatcacc gaggagtggc tctatcacct gggcgtgccc
     2221 gaggctgaaa tccccgacat ggcggcccac tccgccgtct gcgtgccggt catgatgccc
     2281 tatatcaccg ctttctttat gccccgcgcc gccggcgacc ggccggcagt ggtgcccgag
     2341 ggctgcgtca actttgcttt tctcggtcag tttgccgaga ccccgcggga caccatcttc
     2401 accaccgaat actcggtgcg caccgcgatg gaggccgttt ataccctgct ggacgtggat
     2461 cggggcgtgc cggaggtgtg ggggagcgtt tacgacgtgc gcgccctgct ggacgcgacc
     2521 gtgaagatga tggacggcaa aaagctcacc gacgtcaagc tgccctttgc agcgcagctg
     2581 gtggagaaaa aagcgctcaa ggccgtggag ggcacggtgg tggaagagct gctgcgccgc
     2641 tatcacgcca tctgacattc cgttttttgt tttttaaata gaaaggtcgg gctttcctca
     2701 tcggcaaggg aaagcccggc cttttttctc ttgcttcgca acaaacagga aaccgtctca
     2761 gcggccccgt tcccgccgct gtgcggtact ttccccggca attaaaaagg cagccgcaga
     2821 gccgcggctg tcttttgttg ctttgcaata aaaaacttat ttctatagcc ccggcccggg
     2881 gacgggccgg atggctaaaa aaatagccgc tgcgcggcca ttttttatcc cttatgcgtc
     2941 cggtaaaatg ctttcagctc cgctttgacc tccggcggca gctgtgcttt tttcacgccc
     3001 cgcaccgccc cctccagcgc gctgaggcgg tggaggcaag cggagggatc gtccgcctgg
     3061 atgcgcagcc acgcctcccg gctgcccagg gcccgcagct gttccggcgt atcgaccccg
     3121 gcacgggcca gctgacgctc caactccggg ccgatgttgg gcagatcgga aagtttttct
     3181 accatgcact cctccttttt gttgccgcgc cgcaaaagcg gacgcccttt tttctctttt
     3241 cgccgtgcgc tcagggcgtc tgctccgtct gcgtcttttt tgcggcggca gccgaagcgg
     3301 acagcctgcg ccacatgctc ctgctccgcc gccgcccgcc gtccggaggc ggctcgggcg
     3361 gacaggcccg tgacggctgc cgcgccgttt tttctatcat agcacagggg gcggcggaaa
     3421 gaaagaagag gaagcggcag acaaagaaaa actgcgtttc gcccccgccg ctcaggcgcc
     3481 gcagcgcgcc gggccggtca tgtgcagcgg ttccagcacc tcttgcagct gctcagcggg
     3541 caacacgccc tcccgctcgg ccagcagccg caccggcacc tcctcccgca ccgcctcttt
     3601 cgccagcgcc gcgctgcgct cgtacccgat gagggggcag agggcggccg cgaccccggc
     3661 gctgcgctcc accatgcggc ggcagttctc ccgctcggcg cggatgccgg tgacacagtg
     3721 atccacaaag gtgcgcaccg cgttgcgcag cagctcggcg gattcaaaaa ggctgtacag
     3781 cagcacaggc tcaaaggcgt tgagctccag ctggcccgcc tcggcggcca gggtgatggt
     3841 ggcgtcgttg cccatgatcc ggaacgccac ctggttcacc acctccggca gcaccgggtt
     3901 caccttgccc ggcatgatgg aggagccgtt ctgccgggcg ggcagcgcga tctcgctgat
     3961 gcccgcccgc ggccccattg agagcagccg caggtcgttg gccagcttgg agaggtccac
     4021 ggcgcaggtc ttgagcagcc ccgagacctc cacaaagcag tcgagattct gggtggcgtc
     4081 gatgaggtcg tccgcccgcc gcaggggcag accggtcagc tccgccaggg cgggcaccac
     4141 ccgctgcaga tagcgcggat cagcgttcag
//
LOCUS       DBMDYA010000182         4161 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5072_length_4161_cov_1.516010, whole genome shotgun sequence.
ACCESSION   DBMDYA010000182 DBMDYA010000000
VERSION     DBMDYA010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_4161_cov_1.516010"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(158..550)
                     /locus_tag="ACZPFK_05710"
     CDS             complement(158..550)
                     /locus_tag="ACZPFK_05710"
                     /inference="COORDINATES: protein motif:HMM:NF037170.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1405839.1"
                     /translation="MALNLVGWNVVLDSDHADELSAFYEKLLGWTRFKGEEYTVLVNL
                     EQTGTPTWITIQQVDEYVPPVWPASPEMQQQMAHLDFHVQDVEEGVKYALACGASLSA
                     YQYDDRWRVMIDPAGHPFCILPPIMPWA"
     gene            complement(1328..1888)
                     /locus_tag="ACZPFK_05715"
     CDS             complement(1328..1888)
                     /locus_tag="ACZPFK_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR1405840.1"
                     /translation="MSEAMQFEDREKFRQWLAGNCRSSAGVWLLFGKAGGPKTLKAGE
                     ALEEALCFGWIDGQMQSMGDKAYKKYFSLRRGNSRWSEKNKALAQSLEARGLMTELGR
                     EKMEEARKDGRWNAPPPAAVTEEQIARLAEQLEGYEPAHTNFQAMPASVRKTYTRAYF
                     DAKTEAGRQRRIAWMVDRLNRNLKPM"
     gene            2479..3522
                     /locus_tag="ACZPFK_05720"
     CDS             2479..3522
                     /locus_tag="ACZPFK_05720"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1405841.1"
                     /translation="MITVTTRPFGALPDGRAVTAYTIANGAGMRVTALDYGAILQSVC
                     VPAAAGPVDVVLGYPALEGYLVSGGYLGALVGRNANRLGGAFIEIDGKRWPVTANEGK
                     KQLHGGLHGFNEKLFSAREVEGGVAFSCLSPDGEEGFPGEVSLTATYTLDETGALTLD
                     YRAAATRDTVVNLTNHSYFNLNGGGDAMGHRLRIDADAITAMDADSLPTGALMPVAGT
                     PFDFRQFHTLAERIDEPHEQLGFGGGYDHNFALNKAPGQLAAVAVLEGDQSGIRLTCS
                     TTCPGLQLYSANFMDKNAAPGKAGRQYRDREAVCLETQYFPNANNCKNFPSTVLRAGA
                     VYHEVTVYTFSAR"
     gene            3598..3798
                     /locus_tag="ACZPFK_05725"
     CDS             3598..3798
                     /locus_tag="ACZPFK_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405842.1"
                     /translation="MKKGFKTLLCLLFFCGIAIAAAMRLLSPGGAAPVAAPLPCAFTP
                     LAAGLLCAAGYLFAKPHIQKNE"
BASE COUNT          750 a         1410 c         1146 g          855 t
ORIGIN      
        1 ccggccagtg gacgggccgg atggccgaaa aaaacaacct attcctatgg ccgtggcccg
       61 aggacgggcc acaggaccga aaaaacagcc tccgctttgc tgcggctctt tttgattgct
      121 tcgcaacaaa aacacttatt ttaatgggct ttcattctca cgcccaaggc atgatcgggg
      181 gcaggataca aaacggatgt cccgcagggt caatcatcac ccgccaacgg tcatcatatt
      241 ggtatgccga taggcttgcg ccgcacgcca aggcatattt cacgccctct tccacatcct
      301 ggacgtgaaa atccagatgc gccatctgct gctgcatctc cggcgacgca ggccacacgg
      361 gcggcacata ctcatccacc tgttgaatgg taatccaggt tggagttccc gtttgttcca
      421 aatttaccag aactgtatac tcttccccct tgaatcgcgt ccagccgagt aatttttcat
      481 aaaatgcgga caattcgtcc gcgtggtcgg aatccaaaac gacattccat cccacaagat
      541 taagtgccac tctaccatcc ctccaagttt tctgatccga ccggcacgcc ttattcgcct
      601 gaggcgtaaa aaccatcacc atcttttttc attttaatat ggccgcctga aaaatgtcaa
      661 acaactttac ctttcgccgt acggtttctc acatagcaaa aatgcgggtt tcataggcct
      721 ttccgagtgg aaaaaggggg ttataactgt caaaccacaa ttgatcttga cggccccggc
      781 caggggactg gccggatggc cgaaaaatag ccgcagagcg gctattttta ttgctccgca
      841 ataaaaaaac ttatttctat ggcctcgttt ctcgccgctc tcgcggcaac cgaaacggat
      901 ggcctaaaaa atagccgcag agcggctatt tttattgctt cgcaataaaa aacttatttc
      961 tatggcctcg tttctcgccg ctgcgcggca accgaaacgg atggccgcaa aaaatagcct
     1021 tacggctatt ttttgcgttt gcgttacgac cgcatgactt tgcgcgactg cgcgccctgc
     1081 ggccgatccc gacgccccgg gggctgtgcg aggatcgaaa gcccgcgccg cccctcttgc
     1141 cccgcaccct gcgggtgttc acgggcacgg ttcctgcccg gtaagagcgg cgggccccgg
     1201 cagcgctttg aatcgttttg cattgcggac aaaaaggcaa aaacggcggc tgcccgccgg
     1261 gcgccttgcg cgccgccgga aagccaaaga gagaacgcgc cttattttct ccgggcaggc
     1321 gcaatcgtca catgggcttc agatttctgt tgagccggtc caccatccag gcgatgcgcc
     1381 tctgccgccc cgcctccgtc ttggcgtcaa aatacgcccg cgtgtaggtt ttgcggacgg
     1441 atgcgggcat ggcctgaaaa tttgtgtggg cgggctcgta tccctccagc tgttcggcca
     1501 agcgggcgat ctgctcctcc gtgacagcgg cgggcggggg cgcgttccac cggccgtctt
     1561 ttcgggcctc ctccatcttc tctctgccga gctcggtcat gaggccccgc gcttccaggc
     1621 tttgggccaa cgccttattt ttttccgacc acctgctgtt tcccctgcgc aaagaaaagt
     1681 atttcttata tgccttgtcg cccatgctct gcatctgccc gtcgatccag ccaaagcaga
     1741 gagcctcttc cagcgcctcc cccgctttca gggtcttcgg accgcccgct ttgccgaaca
     1801 aaagccaaac cccggcgctt gaccggcaat tgccggcgag ccattgccga aatttttctc
     1861 tgtcctcaaa ctgcattgcc tcgctcacga tgcgctctcc tctctgccca ctccgtcaag
     1921 gccgtttttt cttttaattt tttcattata ccctccgggc acgggcctgt caaaaaagct
     1981 ccgcgcccct cgggcgccgc gccgcctccc cgggtctgga aaggcggaat ccgcgcgccc
     2041 tgcgcattga gcctcgtcga ggcgtcgctt tagccccccg tgctcttgcc cgcggcggtc
     2101 gctgcaaggc tcccctttcc agcagaccgg agcgcctctc tctgcggcgc gtcaaattcg
     2161 gtcaacggcc tctgcacgcg cgtctccctc cctcgcagct tcaccgcgcc gcttttttcc
     2221 ctgccggcag aggcgcggtc cgcgcgtctt cggcactgcg cccttgcccc ggacagcgcg
     2281 ccccatggcc gcctctcacg cccggggcgg caaaaaaccg cccgggcccc ggctttgcgg
     2341 ccccgcccgg cgcggcggtg cggacagggc aaaacgcgcg cttcgccccc tctcccgatt
     2401 gacattcccc cggcgggcgg gtacactagg attgatcggt tttgccgtat tcttcaaaat
     2461 ttgagggagg ttttttcctt gatcacagtc accacccgcc cctttggggc gctgccggac
     2521 ggaagagccg tcacggccta taccatcgcc aacggcgcgg gcatgcgggt caccgcactg
     2581 gattacggcg ccatcctgca gagcgtctgc gtccccgcgg ccgccggccc ggtggacgtg
     2641 gtgctgggct atccggccct ggagggctat cttgtcagcg gcggctatct cggcgcgctg
     2701 gtgggccgca acgccaaccg gctgggcggc gctttcatcg agatcgacgg caagcgctgg
     2761 cccgtcaccg ccaacgaggg caaaaagcag ctgcacggcg gcctgcacgg cttcaatgaa
     2821 aagctgtttt ccgcccgcga ggtggagggc ggcgtggcgt tctcctgcct gagcccggac
     2881 ggggaggagg gcttccccgg cgaggtctcc ctcaccgcca cctacacgct ggacgagacg
     2941 ggcgccctga cgctggacta ccgcgccgcc gccacccggg acacggtggt caacctcacc
     3001 aaccacagct atttcaacct caacggcggc ggcgacgcca tggggcaccg gctgcgcatc
     3061 gatgcggacg ccatcaccgc catggacgcc gactcgctgc ccaccggcgc gctgatgccg
     3121 gtggcgggca ccccctttga cttccgccag ttccacaccc tggccgagcg catcgatgaa
     3181 ccgcacgaac agttgggctt cgggggcggc tacgaccaca acttcgcgct gaacaaggcg
     3241 cccggtcagc tggccgccgt cgccgtgctg gagggggacc agagcggcat ccggctcacc
     3301 tgcagcacca cctgccccgg cctgcagctc tacagcgcca acttcatgga caaaaacgcc
     3361 gcccccggca aggcggggcg gcagtatcgc gaccgcgagg cggtctgcct ggaaacccag
     3421 tacttcccca acgccaataa ctgcaaaaac tttccctcca cggtgctgcg ggcgggcgcg
     3481 gtctatcacg aggtcaccgt ctacactttt tcagcccgct gaggcttttg gcaggccccg
     3541 gcgccgggca ggcaaaggcc tatgaaccgc cgcatcgttg aaagggggag gcttgccgtg
     3601 aaaaagggat tcaaaacgct gctctgcctg ctctttttct gcggcatcgc catcgccgcg
     3661 gccatgcgcc tgctctcccc cggcggcgct gccccggtgg ccgcgccctt gccctgcgcc
     3721 tttaccccgt tggcggcggg cctgctctgc gccgccggtt atctgtttgc caagccccac
     3781 atccaaaaaa acgaatagaa agatcttctc tcccgccgga tcgggagggg aagaccgcga
     3841 tgtcgcgcgg gcggaccctt tgcgcagtcc cttgtgcggg gccgcccctg cggtgctgcg
     3901 ctgctccgcg ccgggtctgt gaagatgcag acacggcagg gccgctggac gccaaaatca
     3961 aacgggcgca aaccggccca tcgctgtttt ctcctcttcc tccggccttt ttcttctttt
     4021 ctacaataca gaggagattt tttatgaaaa atattacaaa acgtatcggc tttgtcgccg
     4081 cttgcaccgg ccttgtcgcc tttgtggtct acaccgcctg ctttacggcc atcctctttg
     4141 tcaacgagcc ctatacctgg a
//
LOCUS       DBMDYA010000183         4142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5094_length_4142_cov_1.683247, whole genome shotgun sequence.
ACCESSION   DBMDYA010000183 DBMDYA010000000
VERSION     DBMDYA010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_4142_cov_1.683247"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="ACZPFK_05730"
     CDS             <1..776
                     /locus_tag="ACZPFK_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1405843.1"
                     /translation="GAIQNNSTAEFHQKYRAADVLLVDDIQFIGGKDSTQEEFFHTFN
                     TLYNAGKQIVLASDRPPKEIKSLEDRLRTRFEWGLTADIQPPDFETRVAIIRRKADLL
                     GLDIPNDVAEFIANHLKNNIRQLEGAVKKLNAYRLLEGIQPVIGAAQNAIKDILNENQ
                     PVPVTIEKIVSEVARTYNITPADIRSRKRSSNISAARKTAMYVVHEITGMTMQDIGKE
                     FGGRDHSTVVYSISEVEKKIDADPRFKEIVEDIVKNARV"
     gene            1148..2281
                     /gene="dnaN"
                     /locus_tag="ACZPFK_05735"
     CDS             1148..2281
                     /gene="dnaN"
                     /locus_tag="ACZPFK_05735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1405844.1"
                     /translation="MKFECEKSSLAEAIAGVSRAVTMRSSVPALEGILFKAEGNTLTL
                     TGYDLEMGITTKIDARIRDEGAVVLSAKLVGDMVRRLPAELVEIECSENLVTTVKGGI
                     TEYNITGISAADYPELPAVAFQQGGEENKNGVTPAIAMDGPILKEMIETTIYAVSQDD
                     KKPANNGEKFVLESDKLTLVALDGFRLAICERPVTAGREVSIVIPAKTLNEVARLIGD
                     SDEAVAISASKRYVVFTLGGYTVISRLLDDAFLDYKKTINPSHLTRVVVEVKEFSDAI
                     ERASLIITERLKNPLRITFDENLTVRCQTELGKVMDSIPCEFEGEKMEVGFNNRYLLD
                     ALRNSGSDKVAFELNGPLSPVKVLPVEGDDFIFLVMPVRFKND"
     gene            2292..2510
                     /locus_tag="ACZPFK_05740"
     CDS             2292..2510
                     /locus_tag="ACZPFK_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1405845.1"
                     /translation="MTQETIPIRTEFIKLDSLLKFANILPTGGEAKLAIQQGAVSVNG
                     EVCTQRGKKVRPGDRVCIEEFELQVVQA"
     gene            2507..3613
                     /gene="recF"
                     /locus_tag="ACZPFK_05745"
     CDS             2507..3613
                     /gene="recF"
                     /locus_tag="ACZPFK_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565049.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1405846.1"
                     /translation="MILKEHAVRDYRNIEAVRFCPSPRLTVICGKNGQGKTNLLESIW
                     LLSGAKSFRGGKDVQLVRRGQPFAVIESRAQSDDGEEKAYRLVVGGESCAEAGVRPGR
                     SAQLDGVDFGRATNLAGNFYAVVFAPTHLSLVKGSPEGRRRFVDAALCQLYPGYIAVY
                     RRYTRALAQKNALLRDARRFEGLEELLDTFDAALAEAGGEMIRRRREYLEGLFSIASE
                     TYDSLSAGSESCAFFYEPSAADGEDLLQRIRAARPADLRAGCCTVGPHREDVGIRIGG
                     EDARAFGSQGQQRSAALSMKLAEARQIALVTGSEPVMLLDDVLSELDGARRDFLLGRI
                     EGRQVLLTSCDGAVFHDTPGAIWEMEGGRLRQVQ"
     gene            3712..3978
                     /gene="remB"
                     /locus_tag="ACZPFK_05750"
     CDS             3712..3978
                     /gene="remB"
                     /locus_tag="ACZPFK_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix regulator RemB"
                     /protein_id="HIR1405847.1"
                     /translation="MYLHLGTDTIIRTKDILGIFDLDTSTISKRTRDTLRRAELEGRV
                     INVSSDLPKSFIVMAGKRPTIYISQISASTLKKRSGYIDDIKNI"
BASE COUNT          874 a         1310 c         1267 g          691 t
ORIGIN      
        1 tcggggctat ccagaacaac tccaccgccg agttccacca gaaataccgc gccgccgacg
       61 tgctgctggt ggacgatatc cagttcatcg gcggcaagga ctccacccag gaggagtttt
      121 tccacacttt caacaccctc tacaacgccg gcaagcagat cgtgctggcc agcgaccggc
      181 cgcccaagga gatcaaaagc ctggaggacc ggctgcgcac ccgttttgag tggggtctga
      241 ccgcggatat ccagccgccc gattttgaga cccgggtggc gatcatccgc cgcaaggccg
      301 atttgctggg tctcgatatc ccgaacgatg tggccgaatt cattgccaac cacctgaaaa
      361 acaacatccg gcaactggag ggcgcggtga aaaagctgaa cgcctaccgc ctgctggagg
      421 gcatccagcc ggtgatcggc gcagcccaaa acgccatcaa ggacatcctc aacgaaaacc
      481 agcccgtgcc ggtcaccatt gaaaagatcg tcagcgaggt agcccgcacc tacaacatca
      541 ccccggcgga catccgcagc cgcaagcgca gcagcaacat ctccgccgcc cgcaaaaccg
      601 cgatgtatgt ggtgcacgag atcaccggca tgacgatgca ggacatcggc aaggagttcg
      661 gcggccgcga ccactccacg gtggtctact ccatctcaga ggtggaaaaa aagatcgacg
      721 ccgatccccg cttcaaagag atcgtggagg acatcgtcaa aaacgcgcgg gtctgaggcc
      781 gagcgaagcg cccgaaaccc gaagaaagct cccttgtaca cggcgcttcc ttttcctctt
      841 ttttaaccct gccctttctt gaaaaccgcc tttttctttt ccacatccct ttcaacattc
      901 cacactcttt tttccacatc ccctttcaaa aacaggccgg atgtggaaaa ccacagattg
      961 tccacatgtt caccaacaga aaaagaagtc ggttttttcc cggtgcgtca aagcgccgcg
     1021 cgtttttttc cacaaattca cagcccctat tactgctact atttttaaag ctcaataaaa
     1081 gagttttttt gaaaaaataa gaagaaacgc ggccatccgg ccgcaaagac aggttgggag
     1141 gatccgtatg aaattcgaat gcgaaaaaag cagtctcgcc gaggccatcg ccggcgtctc
     1201 ccgcgcggtc acgatgcgca gcagcgtgcc ggcgctggag ggcatcctgt tcaaggcgga
     1261 gggcaatacc ctcaccctga ccggctatga cctggagatg ggcatcacca ccaagatcga
     1321 cgcccgcatc cgggacgagg gcgccgtggt gctcagcgcc aagctggttg gcgacatggt
     1381 gcgccgcctg cccgccgagc tggtggagat cgagtgcagc gaaaacctgg tcaccaccgt
     1441 caagggcggc atcaccgagt acaatatcac cggcatctcg gccgccgatt accccgagct
     1501 acccgcggtg gcctttcagc agggcggcga ggagaacaaa aacggcgtga cgcccgccat
     1561 cgccatggac ggccccatcc tgaaagagat gatcgagacc accatctatg cggtctctca
     1621 ggacgacaaa aaaccggcca acaacggcga aaagttcgtg ttggagagcg acaagctgac
     1681 cctggtggcg ctggacggct tccggctggc catctgcgag cgcccggtca ccgccggccg
     1741 cgaggtctcc atcgtcatcc ccgccaaaac gctgaatgag gtcgcccggc tgatcgggga
     1801 ttccgacgag gccgtggcca tcagcgccag caagcggtat gtggtcttca cgctgggcgg
     1861 ctacaccgtc atcagccgcc tgctggacga cgcctttctg gattataaaa agaccatcaa
     1921 tcccagccac ctcacccgtg tggtggtgga ggtcaaggaa ttctcagacg ccatcgagcg
     1981 cgccagcctg atcatcaccg agcggctgaa aaacccgctg cggatcacct ttgacgaaaa
     2041 cctcaccgtc cgctgccaga ccgagctggg caaggtgatg gactccatcc cctgcgaatt
     2101 tgaaggcgaa aagatggagg tcggcttcaa caaccgctat ctgctggacg cgctgcgcaa
     2161 ctcgggcagc gacaaggtgg cattcgagct caacggcccc ctctccccgg tcaaggtcct
     2221 gccggtggag ggcgacgatt tcatcttcct cgtcatgccg gtgcgcttca aaaacgattg
     2281 aggtgacgga tatgacgcag gagacgatcc ccatccggac agagttcatc aagctcgact
     2341 cccttttgaa atttgccaat atcctgccca ccggcggcga ggcaaagctc gccatccagc
     2401 agggcgccgt gtcggtcaac ggggaggtct gcacccagcg gggcaaaaag gtccgccccg
     2461 gcgaccgggt gtgcatcgag gagtttgagc tgcaggtcgt gcaggcgtga tcctcaaaga
     2521 gcacgcggtg cgcgactacc gcaacatcga ggccgtccgt ttttgcccca gcccgcgcct
     2581 gaccgtcatc tgcggcaaaa acgggcaggg caagaccaat ctgctggaga gcatctggct
     2641 gttgtccggc gccaagagct tccgcggcgg caaggacgtg cagctggtgc ggcgcggcca
     2701 gccctttgcg gtgatagagt cccgggcgca gagcgacgac ggcgaagaaa aagcctatcg
     2761 cctggtcgtg ggcggcgaga gctgcgcgga agcgggggtg cgtcccgggc gcagcgcgca
     2821 gctggacggg gtggactttg gccgcgcgac caaccttgcg ggcaattttt acgcggtggt
     2881 cttcgccccc acccacctca gcctggtcaa gggcagcccc gaggggcgcc gccggtttgt
     2941 ggacgcggcg ctctgccagc tctatcccgg ctatatcgcg gtctatcgcc gctacacccg
     3001 ggcgctcgcc caaaaaaacg ccctgctgcg ggacgccagg cgctttgagg ggctggaaga
     3061 gctgctggac acctttgacg cggcgctggc ggaggcgggc ggcgagatga tccgccgccg
     3121 gcgggagtat ctggaggggc tgttctccat cgcctccgag acctatgaca gcctgtccgc
     3181 gggcagtgag agctgcgcct ttttctatga gcccagcgcc gccgacggcg aggatctctt
     3241 acagcgcatc cgcgccgccc gcccggccga cctgcgcgcg gggtgctgta ccgtgggccc
     3301 ccaccgggag gacgtgggca tccgcatcgg cggcgaggac gcccgcgcgt tcggcagcca
     3361 gggccagcag cgcagcgcgg ccctctcgat gaagctggcg gaggcgcgcc agatcgccct
     3421 ggtcaccggc agcgagccgg tgatgctgct ggacgacgtg ctgtccgagc tggacggcgc
     3481 gcggcgggat tttttgctgg gccgcatcga gggccggcag gtgctgctca ccagctgcga
     3541 cggggcggtt tttcacgaca cgccgggcgc catctgggag atggagggcg gcaggctgcg
     3601 gcaggtgcag tgagaccggg caagaatatc cggcgagggc aaaaaaacgg ctgtttttgg
     3661 caacactagc tgcaggggcg gggcgacccg ccccgggaga gggagcgagc gatgtatctt
     3721 catcttggca ccgacaccat catccgcacc aaggatatcc tggggatttt tgacctggat
     3781 acctccacca tctcaaagcg cacgcgggac accctgcggc gcgcagagtt ggagggccgg
     3841 gtgatcaacg tctccagcga tctgcccaaa agctttatcg tcatggcggg caagcggccc
     3901 accatctata tctcacagat ctcggcctcg accctcaaaa agcggtccgg ctatatcgac
     3961 gacatcaaaa atatctgagc gcgccgctcg gcggcgggga tcggcttttc cgccgccgct
     4021 cgcgctcagg tctcacatag gcttacagga cgcgcggcgg ccccgccggg gacagatcag
     4081 gcaggccgcc cgccgaaacg ggaggaaagc agagagtatg gcagagaacg aaaacaggat
     4141 cg
//
LOCUS       DBMDYA010000184         4106 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5136_length_4106_cov_1.553308, whole genome shotgun sequence.
ACCESSION   DBMDYA010000184 DBMDYA010000000
VERSION     DBMDYA010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_4106_cov_1.553308"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="ACZPFK_05755"
     CDS             complement(<1..538)
                     /locus_tag="ACZPFK_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1405848.1"
                     /translation="MHTLLWFLYFWLYLLAVLPLYWRVKALARRGDTGRHDALTRRVV
                     GRWARRMLWAACAKVEVDGLENLPQGPAVYVANHQGYFDIPTVLGYLGDDTKPMVAKK
                     EIDRIPLIRAWMRQLHCVFLDRENPRSAVATLNEAAKWVGAGYSMVVFPEGTRSKTGE
                     VAEFKAGAFKIAQKNKAPV"
     gene            870..1226
                     /locus_tag="ACZPFK_05760"
     CDS             870..1226
                     /locus_tag="ACZPFK_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1405849.1"
                     /translation="MRFNHFNFNVLDLEKSLAFYKEALDLEPVRKKEGDGFTLAYLGD
                     GVSDFTLELTWMHGRTEKYDLGECEFHLALTTAEFAAAHKKHEEMGCICFENPAMGLY
                     FIEDPDGYWIEIVPER"
     gene            complement(1476..2129)
                     /locus_tag="ACZPFK_05765"
     CDS             complement(1476..2129)
                     /locus_tag="ACZPFK_05765"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1405850.1"
                     /translation="MALKRFSLRPTGLLTIGQMEHRLADFAARGWLLCGANSMFDLFE
                     RAQPAQMRYRLEPSQQPGPGDPPPYEKQQLCREAGWEYIATADKLHIYASPAQAQARE
                     LHTDPAELAAMMDSYRSYYTTQLGAAGLLAVLSTFFLLSRRARQGLWSPWTAAFFLLL
                     SVWMAFRLIFLIHSLWRLQKDLAEGRPMDHQPPYKRTLWGAWAITLILLAVGLLLLF"
     gene            complement(2153..3325)
                     /locus_tag="ACZPFK_05770"
     CDS             complement(2153..3325)
                     /locus_tag="ACZPFK_05770"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1405851.1"
                     /translation="MTEKTENRSPRRRWRMHPSLVLERVGGVENYYAQQARKGLLLQS
                     VGSFLDCFSVEAPREMRYRAEPCLDRREAKEGPDYDRRAFYAEAGWTYLGTGGELHFY
                     SSPADSGAQELHTDPQDFAPVLRRQRRNSFWSMLVLGLLLLVLLGMIAAGYPPRPSDL
                     FQLALLALCLGLLLPGLVRDGLWLAHKRWLRLPPLDHEAAVPRGLIWRNRLLPVLLVI
                     ALNLALPLVQDSIDRSLCKPLDEAAPGQLLLTLADTDGPGLVPVNGHLTARRPDNTFC
                     AFSTVLGIRFCETSETMEQSEGDLRRLSLEHADCRSEAQAAWVLGRMLDRYGWAGNFA
                     PVQAEGLDEAYLNTHIPYWYLARRGRQAVCVYYDGALSPGELFALLAQRMAAREAG"
     gene            complement(3322..3642)
                     /locus_tag="ACZPFK_05775"
     CDS             complement(3322..3642)
                     /locus_tag="ACZPFK_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1405852.1"
                     /translation="MGYTGGPMTEAMYYVLLALLEPSHGYRLMQAVGEVSNGRVKMGP
                     GTLYGVLSRLEEDGLIRRLPDRITRRKTYELTPDGREALFSEYHRLQEMIRDMQRMRG
                     GDPQ"
BASE COUNT          754 a         1329 c         1362 g          661 t
ORIGIN      
        1 ctaccggcgc tttgttcttc tgcgcgatct tgaaagcgcc ggccttgaac tccgccacct
       61 ctccggtctt gctgcgggtc ccctcgggga agaccaccat cgaatagccc gcgcccaccc
      121 atttggccgc ctcgttcagg gtggcgacgg cgctgcgcgg attttcccgg tcgaggaaca
      181 cgcagtgcag ctgccgcatc caggcgcgga tgagggggat cctgtcgatc tcctttttcg
      241 ccaccatggg cttggtgtcg tcgccaagat accccagcac ggtggggatg tcgaaatagc
      301 cctggtggtt ggccacatag acggccgggc cctggggcag attttccagg ccgtccacct
      361 ccactttggc gcaggcggcc cacagcatcc gccgcgccca gcggcccacc acccggcggg
      421 tgagggcgtc gtggcggccg gtgtccccgc gccgggccag ggccttgacc cgccagtaaa
      481 gcggcagtac ggccaacagg tagagccaga aatacaaaaa ccacagcagc gtatgcataa
      541 gcccatccat tccgccgctt cgcgccggca taaattgtaa gggaggcagg cgccttgccg
      601 cgcgaagcag aaaaccggca agacgctccc ctgcaggggg gcgcggatcg ctttccgcga
      661 agcctcccgc aggggatgcg ccgaacatta taatatcata ttttgcgctg gcgggaaacc
      721 gcttttcatg atataatggg gccgccgaca ggcaaagcat cacgaaaaca caagcggttt
      781 ttgcatcgga cggcgcggag gacaagcggg cgggtctcag cccgggccgc ggccccgccg
      841 ccgggcagag gagaggagcg ttggacccca tgagattcaa tcatttcaat ttcaatgtgc
      901 tggacctgga aaagagcctt gccttttaca aggaggcttt ggatctggag ccggtgcgaa
      961 aaaaagaggg ggacggcttt acgctggctt atctgggcga tggggtgagc gatttcacgc
     1021 tggagctgac ctggatgcac ggccggacgg aaaaatacga tctgggcgag tgcgaattcc
     1081 acctcgccct caccacggcg gagtttgccg ccgcccacaa aaagcatgag gagatgggct
     1141 gcatctgctt tgaaaacccc gccatgggcc tctattttat cgaggacccg gacggctatt
     1201 ggatcgagat cgtgcccgag cgttgagaga cggcccgggc cagacgggac aaaatcgaaa
     1261 gaaagcgcag ggccctgccg ccgtggcagg gccctgcgct ttcctttttt aactccgcgg
     1321 agaggtctgc tgggccggtg acggccgcct ctggggcgcg cgacgccgga ggcggtgccc
     1381 cggcagagcc tgtcccttgc ccgcgcgcag cccctgacgc gcatccccgc agggaaacag
     1441 cgtggccggg caaggccggc cggaaagggc ggggctcaga agagcagcag aagccccact
     1501 gccagcagga tgagggtgat cgcccaggcg ccccacagcg tccgtttgta gggcggctga
     1561 tgatccatgg gccgtccctc ggccaggtct ttttgcagcc gccataagct gtggatcaaa
     1621 aaaatgagac gaaaggccat ccacacgctc aacagcagaa aaaacgctgc tgtccagggg
     1681 ctccacagcc cctgccgcgc ccgacgggag agcaaaaaga aagtgctgag cacagccagc
     1741 agccccgccg cgccgagctg ggtggtgtaa taactgcggt agctgtccat catggccgcg
     1801 agctccgccg gatcggtgtg cagctcccgc gcctgtgcct gcgccgggct ggcgtagatg
     1861 tgcagcttgt ccgcggtggc gatgtactcc cagcccgcct cccggcacag ctgctgtttc
     1921 tcatagggcg gcgggtcgcc gggccccggc tgctgcgagg gctccagccg gtagcgcatt
     1981 tgggccggct gggcgcgctc aaagaggtcg aacatgctgt tcgccccgca caaaagccag
     2041 ccgcgggcgg caaagtcggc cagccgatgt tccatctgtc cgatggtgag caagccggtg
     2101 ggccgcaggg aaaaccgttt cagtgccatg ctttttcctc ctgttctgcc gattatccgg
     2161 cctcacgggc cgccattcgc tgcgccagca gggcgaacag ctcgccgggg gagagagcgc
     2221 cgtcgtaata gacgcacacc gcctgcctgc cccgccgggc gagataccag taggggatgt
     2281 gggtgttgag ataagcctcg tccaggccct cggcctgcac cggggcaaag ttaccggccc
     2341 agccgtagcg gtccagcatc ctgcccagca cccaggccgc ctgggcctcg ctgcgacagt
     2401 cggcgtgctc cagtgagagg cgccgcaggt cgccctcgct ttgctccatt gtctcgctgg
     2461 tctcgcagaa ccgaatgccc aaaacggtgg aaaaggcgca gaaagtgttg tccggccggc
     2521 gggcggtcag atggccgttg accggcacga gccccggccc gtcggtgtcg gccagggtga
     2581 gcagcagctg gccgggggcc gcttcgtcca agggcttgca gaggctgcgg tcaatgctgt
     2641 cctgtaccag cggcagggcg aggttgagcg cgatgaccag cagcacgggc agcagccggt
     2701 tgcgccagat gaggccgcgg ggcacggcgg cctcgtgatc caacggcggc aggcgcagcc
     2761 aacgcttgtg ggccagccac aagccgtccc gcaccagccc gggcagcagc agccccaggc
     2821 agagggccag cagcgccagc tgaaacaggt cgctggggcg gggaggatac ccagcggcga
     2881 tcatgccgag cagaacgagc agcaggaggc ccagcaccag catcgaccag aaactgttcc
     2941 ggcgctggcg gcgcagcacg ggggcaaagt cctgcgggtc ggtgtgcagc tcctgcgcgc
     3001 ccgaatccgc cggactgctg tagaaatgca gctccccgcc ggtgccgaga taggtccagc
     3061 ccgcctcggc gtaaaaagcg cgccggtcat agtcagggcc ctccttggcc tcccgccggt
     3121 ccaggcaggg ctcggcccgg tagcgcatct cccggggcgc ctccaccgaa aagcagtcca
     3181 aaaagctgcc cacgctctgc agcagcaggc ccttgcgcgc ctgctgggcg tagtagtttt
     3241 ccactccgcc cacccgctcg agcaccagac tggggtgcat gcgccagcgg cggcgcgggc
     3301 tgcggttttc tgttttctcc gtcattgcgg atcgcctccc ctcatgcgct gcatgtcccg
     3361 gatcatctcc tgcagccggt ggtattcgct gaacagcgcc tcccgcccgt ccggcgtcag
     3421 ctcataggtc tttcgccggg tgatgcggtc cggcaggcgg cggatgaggc cgtcctcctc
     3481 cagccgcgac agcaccccgt acagggtgcc ggggcccatc ttcacccggc cgttcgacac
     3541 ctcgcccacc gcctgcatca gccggtagcc gtggcttggc tccaacaggg ccagcagcac
     3601 atagtacatc gcctcggtca tggggccgcc ggtatagccc atgtcgcccg cccccctttg
     3661 ttatttatta cgtgatacga tagtatgcga tgcgatatat cgtctaacat aatagtagac
     3721 ggcgacaaag gggatgtcaa gccgcagcct gcataaagag aaagagaaaa tcctgtgcag
     3781 aacggcaaaa agcggcggag ggacctcgcg cggcctgagc ggcttttaca gcttttgcgg
     3841 cccttgcggc ctcctgcgaa tgccggggcg gcaacatttt ttggcagcgc gggcgcaggg
     3901 gcgccgttgc gggcaaaaaa atcagccctt acggcgtccg cgacgccggg tggggcaggg
     3961 ggcggcacgc ggggacgaga aaaaacagcg accgaagggg ccgcagcctt tcgccgcgac
     4021 cggagggctc cctgtccccg ctctttggcg gatgggcggc cggggcgtgg gccgccgatt
     4081 tgccgaaagg catggacagc tgccga
//
LOCUS       DBMDYA010000185         4101 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5146_length_4101_cov_1.155500, whole genome shotgun sequence.
ACCESSION   DBMDYA010000185 DBMDYA010000000
VERSION     DBMDYA010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5146_length_4101_cov_1.155500"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="ACZPFK_05780"
     CDS             <1..1779
                     /locus_tag="ACZPFK_05780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1405853.1"
                     /translation="LVGRAAHLIEVRGAPAAAITAVTFTNKAAAELRARLEQRLGKRR
                     CKGLTVGTFHAIALRQLAAAGAAPALLEEAEALRIAAEVLAAEGARGSAKKFLAWVSE
                     VKNGRAAAGDGAFPEACFRAYADRLAALGACDFDDLLLGALRLFEGGAAEAEPLRQGL
                     RHLLIDEFQDIDPLQYRLVQAWSAQSESVFIIGDPDQSIYRFRGAEPGCFDRFLADHG
                     GLVPLHLDRNYRSSPEVLACAAAAIARNPAPAGGRALSPQRPSGPAVRLLTAPDELAE
                     AIFVAKEVARMAGGVDMLGAHTRGRREQAAVEGFSDIAVLCRTHRQLETVEYALATDG
                     IPYLVTGREAYLADPAVRGALGFFRALSAPGDLAALRACLTGALGASPALADEVLAAL
                     AAAPRPAASDPAALLPDALRQTPPLQRFAALARQYASRLSQRPQALLESWITDAGQTL
                     SPALDRLLCAAADAVDLGAFLQNLQLGEEADLTRPGGKRYPTDAVRLMTLHAAKGLEF
                     PVVFLCGLSAGVLPYDAPGRPADLEEERRLFYVGVTRAQRELLLLAPGEPSPFLAELE
                     GLPLQRSSTLEGRRPATGKQLSLFEL"
     gene            complement(2125..3528)
                     /locus_tag="ACZPFK_05785"
     CDS             complement(2125..3528)
                     /locus_tag="ACZPFK_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1405854.1"
                     /translation="MGEEKQTAGLRAASQSDFSQGTVPANILRLALPMVVAQLINVLY
                     SVVDRMYIGHLPTAAGNALTGVGVTFPILTVVIAFANLFGAGGAPLFSMARGRGDDDE
                     ARRIMGNVFSMLAGTGLLLTALVLLFKRPLLYLFGASDVTYPYADAYITIYLCGSVFV
                     MLGLGMNNFINAQGFARKGMMTVLIGAALNIALDPLFIFVFDMGVRGAALATVLSQFV
                     SALWAVGFLCGRRTLLRLEPRWMRVQLPVVGRITALGFSSFIMAITNSAVQSVCNATL
                     QTFGGDLYVGVMTVLNSVREVVMTVVSGLTNAAQPVMSFNYGARKYRRVRDGIRFTTA
                     LCIGYTVLVWALLFFFPRVFIAVFNDDPALMEACVPAMHIYFFGFFMMSLQMAGQAVS
                     VALGRSKQAVFFSLLRKAVIVIPLTLLLPRLFGLGVDGVFLAEPISNFIGGAACYVTM
                     LLTIWPELKRRERSEAA"
     gene            complement(3535..>4101)
                     /locus_tag="ACZPFK_05790"
     CDS             complement(3535..>4101)
                     /locus_tag="ACZPFK_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405855.1"
                     /translation="EKSRGGLMPMILQFMDGRFSGGVRALRIEPEGEALVLYWEEDGV
                     TLRLPAGWRRWADGSVCVQGETFLTAARAEVKPDEDDQPVLKIEVCLPEHSSCRRLKL
                     SWEEGGVLLRLDESPSLALAINKVSGQAANTGAKDPVSDLLLGGGYPQYRVRQLIAPR
                     LRGVATHAGDPALAAEAQPAGREITERG"
BASE COUNT          629 a         1509 c         1368 g          595 t
ORIGIN      
        1 ctggtgggcc gggccgccca cctgatcgag gttcgcggcg cgcccgccgc cgccatcacc
       61 gcggtcactt tcaccaacaa ggcggcggcc gagctgcgtg cccggctgga acagcgcctt
      121 ggcaagcggc gctgcaaagg gctgacggtg ggcacctttc acgccattgc cctgcgccag
      181 ctggccgccg ccggggctgc ccccgccctg ctggaggagg ccgaggccct gcgcatcgcc
      241 gccgaggtac tggccgccga gggcgcgcgg ggcagtgcaa aaaagttttt agcctgggtc
      301 tcagaggtca aaaacggccg cgccgcggcc ggggacggcg cttttcccga ggcttgcttt
      361 cgcgcctacg ccgaccgtct ggcggcgctg ggggcctgcg acttcgacga cctgctgctg
      421 ggcgctttgc gcctgttcga gggcggggcc gccgaggccg agccgctgcg gcaggggctg
      481 cgccacctgc tgatcgacga gttccaggac atcgacccgc tgcaataccg gctggtgcag
      541 gcctggagcg cgcagagcga gagcgtcttc atcatcggcg acccggacca gtccatctac
      601 cgcttccggg gcgcggagcc cggctgcttc gaccgctttc tggcggatca cggcggcctt
      661 gtgccgctgc atctggaccg caactaccgc tccagccccg aggtgctggc ctgcgccgcg
      721 gcggccatcg cccgcaaccc cgcgcccgcg ggcggccgcg ccctgtcgcc gcagcggccc
      781 tccggccccg ccgtgcggct gctcaccgcg ccggacgagc tggccgaggc cattttcgtc
      841 gccaaggagg tcgcccgcat ggcgggcggc gtggacatgc tgggcgcgca cacccggggg
      901 cggcgggagc aggccgcggt ggagggcttc tccgacatcg cggtgctctg ccgcacccac
      961 cgccagctgg aaacggtgga atacgcgctg gccaccgacg gcattcccta tctggtgacg
     1021 gggcgggagg cctatctcgc cgacccggcg gtgcggggcg cgctgggctt tttccgcgcc
     1081 ctgtcggccc ccggcgacct ggccgcgctg cgcgcctgcc tcaccggcgc gctaggggcc
     1141 tcgcccgccc tggcggacga ggtgctggcc gcgctggcgg ccgccccccg gcccgcggca
     1201 tccgacccgg ccgcgctgct gcccgacgcg ctgcggcaga ccccgccgct gcaaaggttc
     1261 gccgccctgg cgcggcaata cgcctcccgc ctgtcacagc ggccccaggc cctgctggag
     1321 agctggatca ccgacgcggg ccagaccctc tcccccgctt tggaccggct gctctgcgcc
     1381 gccgccgatg cggtggacct gggcgctttt ctgcaaaacc tgcagctggg ggaggaggcc
     1441 gacctcaccc ggccgggcgg caagcgctac cccaccgacg cggtgcgcct gatgacgctg
     1501 cacgcggcca aggggctgga gttcccggtg gtctttctct gcggcctcag cgccggcgtg
     1561 ctgccctacg acgcgccggg caggccggcg gatctcgagg aggagcgccg cctgttttac
     1621 gtgggcgtca cccgcgccca gcgcgagctg ctgctgctcg ccccgggcga gccctccccc
     1681 tttctggccg agctggaggg cctgccgctg cagcggtcca gcaccctgga gggccgccgc
     1741 cccgccacag gcaaacagct cagcttgttt gagctgtaac ctctttgctc gccgcctttc
     1801 ccccggccag aggctcggcg gcgcgcccgg caagccgtcc gcacaaaaac gaccggtcgc
     1861 atctctgccg agagatgcgg ccggtcgcat tattttgtcg cggcgacgcc gtgtccgccg
     1921 tttttctatc tgaaaaaacg gagtgccgcc caatggcagt caaagcgggc gggccagcgg
     1981 ccctgcgcgg cgcacttttt gcgcctcatc gcagaaaagc cgggggcgaa aggcaggttg
     2041 ctctcgtctc catcaggggt ctcacagcgc accgccgtct ttccggccgt gtttggtgct
     2101 gcgcgcagac ccttccatct ggcctcaggc cgcctcgctc cgctcgcggc gcttcagctc
     2161 cggccagatg gtcagcagca tcgtcacata gcaggcggcg ccgccgataa agttggagat
     2221 gggctcggcc aggaacacgc cgtccacccc cagaccgaac aggcgcggca gcagcagcgt
     2281 cagcgggatc acgatgacgg ccttgcgcag cagcgagaag aagaccgcct gcttggagcg
     2341 gcccagggcc accgacaccg cctgcccggc catctgcagc gacatcatga aaaaaccgaa
     2401 gaaatagatg tgcatggcgg gcacgcaggc ctccatcagc gccgggtcgt cgttgaacac
     2461 cgcgatgaac acgcggggga agaagaacag cagcgcccac accagcaccg tgtagccgat
     2521 gcacagcgcg gtggtgaagc ggatgccgtc ccgcacgcgg cggtacttgc gcgcgccgta
     2581 gttgaagctc atcaccggct gggcggcgtt ggtcaggccg ctcaccaccg tcatcaccac
     2641 ctcgcgcacc gagttgagca ccgtcatgac ccccacgtac aggtcgccgc caaaggtctg
     2701 cagcgtggcg ttgcagacgc tctgcacggc gctgttggtg atcgccatga taaagctgga
     2761 aaagcccagc gccgtgatac gccccaccac cggcagctgc acccgcatcc aacgcggctc
     2821 cagccgcagc agcgtgcgcc tgccgcagag aaagcccacc gcccacaggg ccgagacgaa
     2881 ctgggacagc acggtggcca gcgccgcgcc gcgcaccccc atgtcaaaga caaagatgaa
     2941 cagcgggtcc agggcgatgt tgagggccgc gccgatgagc accgtcatca tgcccttgcg
     3001 cgcaaagccc tgcgcgttga taaagttgtt catgccgaga cccagcatca caaaaacact
     3061 gccgcacaga tagatggtga tataggcgtc ggcataggga taggtgacgt cgctggcccc
     3121 aaagaggtag agcagcggcc gcttgaacag cagcaccagc gcggtcagca gcaggccggt
     3181 gcccgcgagc atcgaaaaga cgttgcccat gatgcggcgg gcctcgtcgt cgtcgccgcg
     3241 cccccgcgcc atcgagaaca gcggcgcgcc gcccgccccg aacaggttgg caaaggcgat
     3301 gaccaccgtc aggatcggaa aggtcacccc caccccggtc agggcgttgc ccgccgcggt
     3361 gggcaggtgg ccgatgtaca tgcggtccac cacgctgtag agcacattga tgagctgggc
     3421 caccaccatg ggcagcgcca gccgcaggat gttggcgggc accgtgccct gcgaaaaatc
     3481 gctctgggac gcagcccgca ggcctgccgt ctgtttttct tcccccatct tcacctatcc
     3541 ccgctctgtg atctcccggc ccgcgggctg cgcctccgcc gccagggccg ggtcgccggc
     3601 gtgggtcgcc acgccccgca gccggggtgc gatcagctgc cgcacccggt attgcggata
     3661 gccgccgccc agcagcaggt cggacaccgg gtctttggcg ccggtgttcg ccgcctgccc
     3721 ggacaccttg ttgatggcga gagccaggct ggggctctcg tccagccgca gcagcacgcc
     3781 gccctcctcc cacgagagct tgaggcggcg gcaggagctg tgctcgggca ggcagacttc
     3841 gatcttcagc accggctggt cgtcctcgtc cggcttcacc tcggcccgcg ccgcggtgag
     3901 aaaggtctct ccctgcacgc agacgctgcc gtccgcccag cgcctccatc cggcgggcag
     3961 ccgcagcgtg acgccgtcct cctcccaata gaggaccagc gcctctccct cgggctcgat
     4021 gcgcagcgcc cgcaccccgc cggaaaagcg gccgtccatg aactgcagga tcatcggcat
     4081 caggccgccg cggctctttt c
//
LOCUS       DBMDYA010000186         4078 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5172_length_4078_cov_1.297209, whole genome shotgun sequence.
ACCESSION   DBMDYA010000186 DBMDYA010000000
VERSION     DBMDYA010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5172_length_4078_cov_1.297209"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="ACZPFK_05795"
     CDS             complement(<1..564)
                     /locus_tag="ACZPFK_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016159.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIR1405856.1"
                     /translation="MINLTKAILHILDGNAGVTVFSAAQLDLADDAAYGYVSKHLEKA
                     AGDPGLHSGEFIGVENEFKIKLSQYAREMLAFEPFSAWIAGQAWEILSNSNRIDSSDL
                     LVAEYFEGEKKMLAVLKCTGKLGYTHMVEVDEEGRQVCRIVNQYDILPGTGQKLDEAA
                     FIEVETGAIHFAEKPRTVNGEDCPLFTK"
     gene            complement(874..2415)
                     /locus_tag="ACZPFK_05800"
     CDS             complement(874..2415)
                     /locus_tag="ACZPFK_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1405857.1"
                     /translation="MKKLLFLKNAVILTGTALLLRAVGMFFRVYIAGQVGDEGMGLYQ
                     LIFTLYNLAITLATSGVTVATTRLAADWLARDRGAPRTLCRRLIFYAFCLGGAAGLLQ
                     FFLARPASTLWLGDARAALSLRILAPSLPFMAVGATLRGYFMARRNVSSSSRAQIFEQ
                     AVRIGVVALLIGKALPYGIETACAAIVLGNTVSEALSCLYMVISWRRDLKRFAPGEKQ
                     KPQHLLRDYGGIVAPITATRGIGSLLVTVENVLVPGCLATFLGARETALAQFGQLKGM
                     AMPVLFFPFSFLSTLSTLLLPEIAEAHSAGKKDRLQALICRTVLITVTLSVLMGGLFR
                     AFAAELGETLYHSSEIGFYIGVLGPVMPFMYLESMVDGILKGLGEQLSTFKYSVCDSV
                     LRILLVLALVPRLGMQGFLFVMLFSNIFTGFLNLHRLIRVTGVRVQWMRWVVKPLFSV
                     GAAWAAYYFAFAVWAAPLLPPLAAFLCGAALMALVYFLFLWLTGCVSPQDFSGMKKSP
                     KSGKA"
     gene            2695..3489
                     /locus_tag="ACZPFK_05805"
     CDS             2695..3489
                     /locus_tag="ACZPFK_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1405858.1"
                     /translation="MANKELLTVGQLAKKMGVTVRTLQYYDRMGLLKPSALSEGGRRL
                     YSSRDMVRLHQILSLKYLGFSLQEIQGQLLGLDTPQQVADMLDRQEQLVRRQISELNT
                     ALEALVAFRGEVLQMKTVDFDRYADIIALLRMHNESYWVLKLFDPALNDHVRRRFTDR
                     PELGAHILESYRSMLEEALALKHAGRTPQSAEAVDLARRWWQMITEFTGGDMSLLPKL
                     MAFNQDKGGWDAGMAEKQKEIDAYMGAMLGAYFQQEGIRIPELEEK"
     gene            3486..>4078
                     /locus_tag="ACZPFK_05810"
     CDS             3486..>4078
                     /locus_tag="ACZPFK_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405859.1"
                     /translation="MMEHAVTVRALRKSYGGREVLHGIDLCVRRGEIFALLGANGAGK
                     TTTLECLEGVRRCDAGEIRIEGGRGVQLQSASLPKNLRAGEALVLFSLWAGAAVDRDQ
                     LTRLGIDAFEKRQYRELSTGQKRRLHLALALVGNPEVLFLDEPTAGLDVEGRAALHAE
                     ILRLRDRGATILLASHDMAEVAQLCDRIAILKEGEIA"
BASE COUNT          810 a         1299 c         1281 g          688 t
ORIGIN      
        1 cttggtgaaa aggggacagt cctccccgtt gacggtgcgc ggcttttccg caaagtggat
       61 cgcccccgtc tccacctcga tgaaagccgc ctcgtccagt ttttgccccg tgccgggcag
      121 gatatcgtac tggttgacga tgcggcagac ctgccgcccc tcctcgtcca cctccaccat
      181 gtgggtatag cccagcttgc cggtgcattt gagcacggcg agcatctttt tttctccctc
      241 aaaatactcg gccaccagca gatcggagga atcgatgcgg tttgaattgg acaggatctc
      301 ccaggcctgc ccggcgatcc aggctgaaaa cggctcaaag gccagcatct cccgggcgta
      361 ttggctcagc ttgattttga actcgttctc caccccgata aattccccac tgtgcaggcc
      421 cgggtcgccg gccgcttttt ccaggtgctt gctgacatag ccataggccg cgtcgtccgc
      481 caggtcgagc tgcgcggcgg aaaacacggt caccccggcg ttgccgtcca aaatatgtaa
      541 aattgctttg gtgaggttga tcatcgtcct taactcccct ttttcacaga acaaaagacg
      601 aacacccgcc tgcggtaaaa gaacccgccc ccggccggat gcccgtcttt ttcttttttt
      661 cacaagacgc tcttgcagta tagcattttt cccctgcttt ttgcaagcgg gcgtcgccgt
      721 ctttccattc cgccccccgg cgcaggcgcc gtcgcgcccc acagtccgct tttgcgttgt
      781 tcccctccac acgcgcccgg cggcggggcg gcggaggtgt ggggcgggag tttttttctc
      841 ccgcacgtcc ctgcgtgacc ccgcgcgcac tgctcaggct ttcccgcttt tcgggctttt
      901 tttcatccct gaaaaatcct gtggggagac gcagccggtg agccagagaa agaggaaata
      961 caccagcgcc atcagcgccg cgccgcagag aaaggccgcc agcggcggca gcagcggcgc
     1021 cgcccagacc gcaaaggcaa aatagtaggc agcccaggcc gcccccaccg aaaacagcgg
     1081 cttcaccacc cagcgcatcc actgcacccg caccccggtc acccggatca gccggtgcag
     1141 attcaaaaaa ccggtaaaga tattggagaa cagcatcacg aacaaaaagc cctgcatgcc
     1201 gaggcgcggc accagcgcca gcaccagcag gatgcgcagc accgaatcgc agacgctgta
     1261 tttaaaggtg gagagctgct cccccagccc tttcaggatg ccgtccacca tgctttcgag
     1321 atacatgaaa ggcatcacgg ggcccaacac cccgatataa aagccgatct cagaggagtg
     1381 gtagagcgtt tcgcccagct cggcggcaaa ggcgcggaac agcccgccca tcagcaccga
     1441 aagggtcacc gtgatgagca ccgtgcggca gatgagcgcc tgcaggcggt cctttttgcc
     1501 cgcgctgtgc gcttcggcaa tctcgggcag cagcagggtg gatagggtgg acaaaaacga
     1561 gaacggaaaa aagagcaccg gcatcgccat gcccttgagc tgaccgaact gggccagggc
     1621 cgtctcccgc gccccgagaa aggtggcaag gcagccgggc accagcacgt tttccaccgt
     1681 caccagcagg ctgccgatgc cccgggtggc ggtgatgggg gccacgatgc ccccgtagtc
     1741 acgcagcaga tgctgcggct tttgtttttc gccgggcgca aagcgcttca ggtcccgccg
     1801 ccagctgatg accatgtaca ggcaggacag cgcctcggac acggtgttgc ccagtacgat
     1861 ggcggcgcag gcggtctcga tgccgtaggg caacgccttg ccgatcagca aagccactac
     1921 cccgatgcgc accgcctgtt caaaaatctg ggcgcgggag gagctgctca cgttgcgccg
     1981 ggccatgaaa tagccgcgca gcgtcgcccc taccgccata aaaggcaggc tgggcgccag
     2041 gatgcgcagc gagagcgcgg cccgcgcgtc gcccagccaa agggtgctgg ccggccgcgc
     2101 caaaaaaaac tgcagcagcc cggccgcccc gcccaggcaa aaggcgtaaa agatcagccg
     2161 ccggcagagc gtccttgggg cgcccctgtc ccgggccagc cagtcggcgg ccaaccgggt
     2221 ggtggccacc gtcacgcccg aggtcgcaag ggtgatggcc aggttgtaaa gggtgaagat
     2281 gagctggtag agccccatcc cctcgtctcc cacctgcccg gcgatataaa cgcggaaaaa
     2341 catccccacc gcccgcagca gcagcgccgt gccggtgagg atgaccgcgt tttttaaaaa
     2401 gagaagcttt ttcaaaccca ttcccccgcc ccgatttgcc gcagagggtg cggcgggggg
     2461 gcgccttgcc cgtcgcccgc cttggccgtg catccctccc gggccccgcc ctcgcgccgc
     2521 gcgcagggga gcggcattcc gcccgccccg cccggccggg taggtctatt cactttcaac
     2581 tatatggcgg aaaaagcgaa aataggcttg tttttccttt ttgataaaat tgaaaaaggg
     2641 cttgcatgtg acgctgcgtc acatgttata ctgcacaaca gggagggaga cgctatggca
     2701 aacaaagaat tgctcaccgt ggggcagctg gcaaaaaaga tgggggtcac ggtgcgcacc
     2761 ctgcaatact acgaccggat gggcctgctg aagccctcgg ccctcagcga ggggggccgc
     2821 aggctgtact cctcgcggga catggtgcgg ctgcaccaga tcctgtccct caaatatctg
     2881 ggcttttcgc tgcaggagat ccagggccag ctgctgggcc tggacacccc gcaacaggtg
     2941 gccgacatgc tggaccgtca agagcagctg gtgcgccgac agatcagcga gctgaacacc
     3001 gccctggagg cgctggtggc gtttcgcggc gaggtgctgc agatgaaaac ggtggatttt
     3061 gaccgctatg cggacatcat cgccctgctg aggatgcaca acgagagcta ttgggtgctg
     3121 aagctgttcg acccggccct gaacgatcat gtgcgccgcc gcttcaccga ccgccccgag
     3181 ctgggcgccc acatcctgga gagctaccgc agcatgctgg aggaggcgct ggcgctgaaa
     3241 cacgcgggcc gcaccccgca gagcgccgag gcggtcgacc tggcccggcg ctggtggcag
     3301 atgatcaccg aattcaccgg cggggacatg agccttttgc ccaagctgat ggcattcaac
     3361 caggacaagg gcggctggga tgccgggatg gcggaaaagc agaaagagat cgacgcgtat
     3421 atgggggcca tgctgggggc ctattttcaa caagagggca tccgcatccc ggaactggag
     3481 gaaaaatgat ggagcatgcg gtgacggtaa gggcactgcg caaaagctac ggcggccgcg
     3541 aggtgctgca cggcatcgac ctctgtgtgc ggcgcgggga gatctttgcg ctgctggggg
     3601 ccaacggcgc gggcaaaacc accaccctgg agtgcctgga gggggtgcgg cgttgcgatg
     3661 cgggcgagat ccgcatagag ggcggccggg gcgtgcagct gcagagcgct tcgctgccca
     3721 aaaacctccg cgccggggaa gcgctggtcc tcttctccct ctgggcgggg gccgctgtgg
     3781 accgggacca gctgacccgc ctggggatcg acgcttttga aaagaggcag taccgggagc
     3841 tttccaccgg ccaaaaacgc cggctacacc tggcgctggc tctggtcggc aaccccgagg
     3901 tgctgttttt agacgagccc accgcggggc tggacgtgga gggtcgggcg gcgctgcacg
     3961 ccgagatcct gcgcctgcgg gacaggggcg ccaccatcct gctggcgagc catgatatgg
     4021 ctgaggtggc acagctctgc gaccgcatcg ccatcctgaa agagggagag attgcctt
//
LOCUS       DBMDYA010000187         4073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5177_length_4073_cov_1.439829, whole genome shotgun sequence.
ACCESSION   DBMDYA010000187 DBMDYA010000000
VERSION     DBMDYA010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5177_length_4073_cov_1.439829"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="ACZPFK_05815"
     CDS             complement(<1..664)
                     /locus_tag="ACZPFK_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1405860.1"
                     /translation="MDKPVLYIVIPCYNEQEVLPITSGLFLQKIKDLAAAGKISDASR
                     VLFVNDGSRDDTWQIIRRLAEEDEHFEGVSLSRNRGHQNALLGGLMTARDRCDVTISI
                     DCDGQDDINAMDAMVDAYLDGCEVVYGVRSKRDTDTFFKRFTAEGFYHLMNWLGAGIV
                     FNHADYRLMSSRVLNEFANYKEVNIFLRGMVPLVGFKSTSVYYERAERMAGESHYPLK
                     KML"
     gene            997..1599
                     /locus_tag="ACZPFK_05820"
     CDS             997..1599
                     /locus_tag="ACZPFK_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405861.1"
                     /translation="MKNNIMDLRWNLVWEPEDTPPLKPRYHSGQVLIKERASLLFLLL
                     LFLLSDLFSALWLHASSSTLLAAAAWEAGALFLFWWGLGVGHDTLWRRGLFRLAKREQ
                     TVCSVFNLFPCLVFAICSAPALKSALIAAGSHTEKTFWCGLGLCAAFAYLFLCWMVYR
                     GHLFVPLFTPAGRKRNLAVFLLLLTLLVICVVELCWSLFR"
     gene            1795..2247
                     /locus_tag="ACZPFK_05825"
     CDS             1795..2247
                     /locus_tag="ACZPFK_05825"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1405862.1"
                     /translation="MGRETVRAYTNQYGKLRDIQYAAYEMYARKHGLTAKELFVLDII
                     WFAPDGCLQSEICERLSVTKQTVSAIIKKFLKQGYVFLTESEADRRNKIVRFTESGLA
                     YVQRIIPPAAEAEVDAMGELSGEEVAELVRLTTLFSQHMKKKFAAIHP"
     gene            2380..2979
                     /locus_tag="ACZPFK_05830"
     CDS             2380..2979
                     /locus_tag="ACZPFK_05830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1405863.1"
                     /translation="MEFYEVIEKRRSIRQFEDREIPKETLERILTAGLKAPSSNHQRR
                     WELVTLTDKALIRELAQFIKPYPCRITEPKTPQQEMFKIAYPRQRTMVEEAACVVLPY
                     FKCKYDLKSTTNDYGLMDYGAAWALIENLLLAATAEGLGSVVHIPVKKEPEQIKKWMS
                     APDGYILPALVLLGYAAQDAQVPAQVKATVENKVHWNKW"
     gene            2973..3893
                     /locus_tag="ACZPFK_05835"
     CDS             2973..3893
                     /locus_tag="ACZPFK_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM mobile pair protein B"
                     /protein_id="HIR1405864.1"
                     /translation="MVTEKRGGAPLIVKEIETKNVLTQSNLPVSDYSVNPYVGCAHAC
                     KYCYASFMKRFTGHSEPWGAFVDVKHWPPILRPERYAGKELFIGSVTDPYQPLEETCK
                     RTRALLEQMQGSGCKISIATKSDLVLRDLDLIKTFPDARVSWSINTLDEDFRKEMDEA
                     VSIERRLAAMKAFHDAGVRTTCFISPIFPGITDPPAIIRRAKAHCNLIWLENLNLRGS
                     YKAVILGYIREKHPDLLALYQAIYQRNDRAYWALLDAQLREFTRAEGLPYVRNDDSMR
                     RAFSAPPVVVNYFFHEQIKKSAKRTGGKNA"
BASE COUNT          808 a         1272 c         1183 g          810 t
ORIGIN      
        1 ccagcatctt tttcagcggg tagtggctct cgcccgccat ccgctcggcc cgctcgtagt
       61 agacgctggt gctcttgaag cccaccagcg gcaccatgcc ccgcaaaaag atgttgacct
      121 ctttgtaatt ggcaaactcg ttcaagaccc ggctgctcat cagccggtag tcggcgtggt
      181 tgaaaacgat gcccgcgccc agccagttca tcagatgata gaacccctcg gcggtgaacc
      241 gcttgaaaaa ggtgtcggtg tcccgcttgg agcgcacgcc gtagaccacc tcgcagccgt
      301 cgagataggc gtcgaccatg gcgtccatgg cgttgatgtc gtcctgtccg tcgcagtcga
      361 tggagatggt cacatcgcag cggtccctgg cggtcatcag gccgcccagc agcgcgttct
      421 ggtgcccgcg gttgcgggag aggctgaccc cctcgaagtg ctcgtcctcc tcggcgaggc
      481 gccggatgat ttgccaggtg tcgtccctgg agccgtcgtt gacgaacagc acccggctgg
      541 cgtcgctgat cttgcccgcc gcggccaaat ccttgatttt ttgcaaaaac agcccgctgg
      601 tgatgggcag cacctcctgc tcgttgtagc aggggatgac gatgtataag accggcttgt
      661 ccatctctgt attccatcct tttattttgt gatcgtcttt tgtcttcggg gccgggcgca
      721 tcgcggggcc gccgttcttt ttactatacc ccattcccgc cccgggcaca acccgttctc
      781 actgaaattt caccggctcc gggatatccg gccgccccgg ctgtcgcggt ccctcccccg
      841 ctgcggcgcg gcgctcaaaa tcggcattac gccaaaaaaa gaagccgtct tttgtggctt
      901 tggaagttta cattctccgt ccgggcctgt aaactgagaa caaaaggcag gtgaaaggca
      961 atgggtgctc cgccgctgcc gcccgggagc tgggcgatga aaaacaatat catggatctg
     1021 cgctggaacc ttgtctggga gccggaggac acgcccccgc tcaagccgcg ttatcacagc
     1081 ggccaggtct tgataaaaga acgcgcatct cttctttttc tgcttttgct ctttttgctc
     1141 tcggatcttt tttcagctct ttggttgcac gcttcgtcct cgaccctcct cgcagctgcc
     1201 gcctgggaag cgggggccct ttttctcttc tggtgggggc tgggggtggg gcacgacacc
     1261 ctttggaggc gcggcctctt ccgcctcgca aagcgggagc agacggtttg cagcgtgttc
     1321 aatctcttcc cctgcctcgt cttcgccatc tgctcggccc cggccctcaa aagcgctctg
     1381 atcgccgccg gctctcacac agaaaaaacg ttctggtgcg gccttgggct gtgcgccgct
     1441 tttgcctatc tgtttttatg ctggatggtc tatcggggcc atctttttgt accgcttttc
     1501 accccggcgg gcagaaagcg gaacctggcg gtttttcttc ttctgctgac gctgcttgtg
     1561 atctgcgtcg tggagctctg ctggtcgctg ttccgctgac ctttcatcgc cgcgctttcc
     1621 gccctccctc cggccatcct tgtggtgccg cccctccggg gcgggggagg ggcggcggcg
     1681 tctttccgcc gggggctttt ctcctcttgc tcgatacgct gttgacaaaa atgaatttct
     1741 ctgttaccat aaagtcaaat attagaccat caaataattt acggaggggt ttttatggga
     1801 cgcgagaccg tcagggcata taccaaccaa tatggcaagc tgcgggatat ccaatatgcc
     1861 gcctatgaga tgtatgcgcg aaagcacggc ctgaccgcaa aggagctgtt tgtgctggat
     1921 atcatctggt ttgcgccgga cggctgcctg cagtccgaga tctgcgagcg gttatcggtg
     1981 accaagcaga ccgtgagcgc catcatcaaa aaatttttga agcagggcta tgtctttctg
     2041 accgaatccg aggcggaccg ccgcaataaa atcgtccgct ttacagagtc ggggctcgca
     2101 tacgtgcagc gcatcatacc gcccgccgct gaggcggagg tagacgccat gggcgaactg
     2161 tcaggggagg aggtcgccga gctggtgcgt ctcacgacgc ttttttccca gcacatgaaa
     2221 aaaaagtttg ccgcgataca tccgtgaccg ggtccggcaa aaaggaagcg gcgcaagggg
     2281 gcccggcgag ggagccggca aaaaagcagc cggcagaaga tgggcgggcc gcacggcaaa
     2341 agagcgcgcg ccccgccgaa agacaagggg gatatgaaaa tggaatttta tgaagtgatc
     2401 gaaaagagaa gaagcatccg tcaatttgag gacagagaga tccccaaaga aacgctggaa
     2461 cggattttga ccgccggctt gaaagccccc tcctccaacc atcagcgccg gtgggagctg
     2521 gtgaccttga ccgacaaggc cctcatccgg gagctggccc agttcatcaa gccctatccc
     2581 tgccgcatca cagagccgaa aacgccccag caggagatgt tcaaaatcgc ctatccccgc
     2641 cagcggacga tggtggagga ggcggcctgc gtcgttctgc cctatttcaa gtgcaagtac
     2701 gacctgaaaa gcaccaccaa cgactacggc ctgatggatt acggcgctgc ctgggcgctc
     2761 atcgagaacc ttctgctggc cgccaccgcc gaggggctcg ggtctgtcgt ccacatcccc
     2821 gtgaagaaag agccggaaca gatcaaaaaa tggatgagcg ccccggacgg gtacattctc
     2881 ccggctctgg tactgctggg ctatgcggcc caggatgccc aggtccccgc ccaggtcaag
     2941 gccacggtag aaaacaaagt ccactggaac aaatggtaac ggagaaacgc ggaggcgccc
     3001 ccctgatcgt caaagagatc gagacgaaaa acgtcctcac ccaatcgaac ctgccggtca
     3061 gcgactactc ggtcaacccc tatgtgggct gcgcccacgc ctgcaagtac tgctacgcca
     3121 gctttatgaa gcggtttacg ggtcacagcg agccctgggg cgcgttcgtc gacgtcaagc
     3181 actggccccc catcctccgc ccggaacggt acgcgggcaa ggagctgttt atcggctccg
     3241 tcaccgaccc ctatcagccg ctggaggaga cctgcaaacg gacccgggcg cttctggagc
     3301 agatgcaggg gagcgggtgc aaaatcagca tcgccaccaa gtctgacctt gtcctgcgcg
     3361 atctcgatct gatcaagacc tttcccgacg cccgcgtgtc ctggtccatc aacaccctgg
     3421 atgaggactt tcgcaaggag atggatgagg cggtgagcat cgagcgccgc ctggccgcca
     3481 tgaaagcctt tcacgacgcc ggggtgcgta cgacctgctt catctcgccc atcttccccg
     3541 gcatcaccga cccgcccgcc atcatccgcc gggccaaagc ccattgcaac cttatctggc
     3601 tggaaaattt gaacctgcgg gggagctata aggcggtcat cctgggctat atccgggaaa
     3661 agcaccccga cctgctggcg ctctatcagg cgatctacca gcgcaacgac cgcgcctact
     3721 gggccctgtt ggatgcgcag ctgcgggaat tcacccgggc ggagggcctc ccctacgtcc
     3781 ggaacgacga ctccatgcgc cgcgcctttt ccgcgccgcc cgtcgtggtc aactattttt
     3841 tccatgagca gatcaaaaaa tcggcaaaga gaacgggcgg caaaaacgcc tgaaccgccg
     3901 aaagcgatcc ccctgcggcc cccccggccg ccccacggcc tgcacggcaa aacgtccaaa
     3961 gcggcggcat ccgcgggcgg cgtgcgccga cgccgtcaag gcggcgggcc gcaagccgcc
     4021 ggtgggggga aagcctcgtc tctacagcgc gcaaaagaac ggccgcggaa cac
//
LOCUS       DBMDYA010000188         4051 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5208_length_4051_cov_1.316709, whole genome shotgun sequence.
ACCESSION   DBMDYA010000188 DBMDYA010000000
VERSION     DBMDYA010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_4051_cov_1.316709"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="ACZPFK_05840"
     CDS             <1..1050
                     /locus_tag="ACZPFK_05840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1405865.1"
                     /translation="FRAWEIPRKGNTGLRIFLNYVSFPFFATFDLLRLHGRRYDAVFC
                     YETSPVLMAFPAIVYGKLHRAPTTIYVLDLWPENLYSVLPVKNGLLRSVASAVSLWHY
                     RAADRLIAMSPGLRQKLIERTGKRPGDVAVVPQYCEALYARDVEDEALRARFAGKFNV
                     VFAGNFSPAQSLETLVEVAALLRARGESGIRFILVGDGMSREALLRRIEDEGLGSYFA
                     YEGQHPAEDVPKYHTLADALVACLAGTEDLGLTIPAKITSYCAAGRPVLASMDGEGAR
                     VVREAGCGFASPAGDARALTENLLRLYRMTPAQRAELGRRGRAYNAAHFDRETLLRQL
                     TDFMLGQEIPPAGDC"
     gene            1073..1780
                     /locus_tag="ACZPFK_05845"
     CDS             1073..1780
                     /locus_tag="ACZPFK_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1405866.1"
                     /translation="MKILVLIPCYNEEENLERVVTRLKEACPEVDYLVINDCSTDRSR
                     EICDEKGYSYISLPVNLGIGGGVQSGYLYAVERGYDVTVQMDGDGQHDPAYLRALVQP
                     VLDGRLDMAIGSRFITKDGFQSSALRRLGINIISLFIRLLTGKRVRDTTSGFRACNRA
                     LTEFYARHYAQDYPEPEAIVAALAAGYRVGEVPVVMEERMGGTSSIRSFKSAYYMVKV
                     CLSLIVYRLSFSRRRRR"
     gene            1816..2226
                     /locus_tag="ACZPFK_05850"
     CDS             1816..2226
                     /locus_tag="ACZPFK_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1405867.1"
                     /translation="MDIMFPAFRLAMILGVLLYLGVIFYLLKKQKLGVQYAIIWLISG
                     FVLLIFALFPYIVLVMGDILHVLNPVNFVFMVILAFVLLILLSLSAVVSGFALKIKSL
                     TQNAALLERRIRELESGQKSAGGDADGPSGPGRL"
     gene            2296..3903
                     /locus_tag="ACZPFK_05855"
     CDS             2296..3903
                     /locus_tag="ACZPFK_05855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1405868.1"
                     /translation="MTETFTRQVQELWRFLQQEIFSQPFTLQSVWDWLGGLLQKGMSF
                     NSLAFLLFFPLCVLLYYVLPKAVRNLWLLVCSYFFYYFATVSGGRAHPAALAVLALLT
                     LATYLLALALDRRAARRPRRLLLAAGVLANIGMLVYVKYTSFFVSLLNGWFGWSLKAA
                     PGLSPVWMIGLSFYTLMAVGYLVDVYRGVSPAEKSPVKCALFLSFFPQVVSGPISRSG
                     NLMHQMNRVHRYDYALVNQGLRQMLWGYFKKLVVSENAAAILKLAFASPGSYNGFQLL
                     VSALLYAVQLYADFSGYSDIALGAARCLGFKLPDNFRRPYGARSIGEFWDRWHISLSS
                     WLRDYLYIPLGGSRRGVARTCANTLIVFWVSGLWHGAGLTFLVWGGLHGLYNVVSRLT
                     KPLRLAVNRLTRLNRCKKLHHFLQRAMTFCMVAFAWIFFRADSLDQALLFISRLPLRL
                     GQTLLSWTAINRALAGIGFYANNGVALLCAILLMFAAEALERRRPLAETVGRQPFLLR
                     WAGYYALLLGILFFGVFSATPFIYGQF"
BASE COUNT          584 a         1355 c         1273 g          839 t
ORIGIN      
        1 tttcgcgcct gggagatccc ccgcaagggg aacaccggcc tgcgcatctt tctcaactat
       61 gtctcttttc cctttttcgc cacgttcgac ctgctgcggc tgcacggccg gcgctacgac
      121 gcggtgttct gctatgagac cagcccggtg ctgatggctt ttccggccat cgtctacggc
      181 aagctgcacc gggcgcccac caccatctat gtgctggacc tctggcccga aaacctttat
      241 tccgtgctac cggtcaaaaa cgggctgctg cgctctgtgg cctccgccgt ctcgctttgg
      301 cattaccggg cggcggaccg gctgatcgcc atgtcccccg ggctgcggca gaagctgatc
      361 gagcgcaccg gcaagcgccc cggcgacgtt gccgtggtgc cccagtactg cgaggccctc
      421 tacgcccggg atgtggagga cgaagcgctg cgcgcccgct ttgccggcaa gttcaacgtg
      481 gtgttcgcgg gcaatttttc ccccgcccaa tcgctggaga ccctggtgga ggtggcggcg
      541 ctgctgcgcg cgcgcgggga aagcggcatc cgctttatcc tggtgggcga cggcatgagc
      601 cgcgaggccc tgctgcggcg gatcgaggac gaggggctgg gctcttattt tgcctacgag
      661 gggcagcacc cggccgagga cgtgcccaaa taccacaccc tggccgacgc gctggtcgcc
      721 tgccttgccg gcaccgagga cctggggctg accatccccg ccaagatcac cagctactgc
      781 gccgccgggc ggccggtgct ggccagcatg gacggcgagg gggcccgggt ggtgcgggag
      841 gcgggctgcg gctttgccag cccggccggg gacgcacggg cgctgaccga gaacctgctg
      901 cggctctacc gcatgacccc ggcccagcgc gccgagctgg gccgccgcgg gcgcgcctac
      961 aacgccgcgc atttcgaccg cgagacgctg ctgcgccagc tcactgattt catgctcggc
     1021 caggagatcc ccccggccgg ggactgctga cccaccggag gtgcatccgc ttttgaaaat
     1081 ccttgtgctc atcccctgct acaatgaaga ggaaaacctg gaacgggtcg tgacccgcct
     1141 gaaagaggcc tgtcccgagg tggactatct ggtcatcaac gactgctcca ccgaccgcag
     1201 ccgcgagatc tgcgacgaaa agggctacag ctacatctcg ctgccggtca acctcggcat
     1261 cggcggcggc gtgcagagcg gctatctcta cgcggtggag cggggctatg acgtgacggt
     1321 gcagatggac ggcgacggcc agcacgaccc cgcctacctg cgcgcgctgg tccagccggt
     1381 gctggacggg cggctggaca tggccatcgg cagccgcttc atcacaaagg acggctttca
     1441 gtccagcgcg ctgcggcggc tgggcatcaa catcatcagc ctgtttatcc ggctgctcac
     1501 cggcaagcgg gtgcgggata ccaccagcgg tttcagggcc tgcaaccggg cgctgaccga
     1561 gttttacgcc cgccactacg cccaggatta ccccgagccg gaggccattg tggccgcgct
     1621 ggcggcgggc taccgggtgg gagaggtgcc ggtggtgatg gaggagcgca tgggcggcac
     1681 ctccagcatc cgctctttca aatcggccta ctatatggtc aaggtctgtc tgtccctgat
     1741 cgtctaccgg ctgtcctttt cccgccgccg caggcggtga gccgcatttt ccccatcctt
     1801 ttgaggtgaa gtaaaatgga tattatgttt ccggctttcc ggctggcgat gatcctgggg
     1861 gtcctcctct atctgggcgt catcttttac ctgctcaaaa agcagaagct gggcgtgcag
     1921 tacgccatca tctggctgat ctccggcttt gtgctgctga tcttcgccct cttcccctac
     1981 atcgtgctgg tgatggggga tatcctgcac gtgctcaacc cggtcaactt cgtctttatg
     2041 gtgattttgg cctttgtgct gctgatcctg ctctccctct cggcggtggt cagcggcttc
     2101 gcgctgaaga tcaaatccct cacccagaac gccgccctgc tggagcggcg catccgcgag
     2161 ctggagagcg ggcagaaaag cgccggcggg gacgcggacg gcccctccgg ccccggccgg
     2221 ctctgaaccg ctttgttgac cccctgcttt acgccgtggc ggcgcggcga atccatgagt
     2281 tgaggttttg gtggtatgac ggagacattc acacggcagg tgcaggagct ctggcgcttt
     2341 ctgcagcagg agatcttcag ccagcctttc acgctgcaat cggtgtggga ctggctgggc
     2401 ggcctgctgc aaaaggggat gagcttcaac tctctggctt ttctgctctt tttcccgctc
     2461 tgcgtgctgc tgtactatgt gctgcccaag gctgtacgca acctctggct gttggtgtgc
     2521 agctattttt tctactattt cgccaccgtc tcgggcggcc gggcccaccc cgccgcgctg
     2581 gcggtgctgg cgctgctcac gctggccacc tatctgctgg cgctcgccct tgaccggcgc
     2641 gcggcgcgcc ggccgcgccg cctgctgctg gcggcggggg tgctcgccaa catcggcatg
     2701 ctggtgtacg tcaagtacac ctcctttttc gtctcgctgc tgaacggctg gttcggctgg
     2761 tccctcaagg ccgcccccgg cctctcgccg gtctggatga tcggcctgag cttttacacc
     2821 ctgatggcgg tgggctatct ggtggacgtg taccggggcg tctccccggc ggagaagagc
     2881 ccggtcaagt gcgcgctgtt cctctcgttt ttcccccagg tggtgtccgg ccccatcagc
     2941 cgcagcggca atctgatgca ccagatgaac cgggtgcacc gctacgatta cgccctggtc
     3001 aaccaggggc tgcggcagat gctgtggggc tatttcaaaa agctggtggt cagcgagaac
     3061 gccgccgcca tcctcaagct ggcctttgcc tcgcccggct cctacaacgg ctttcagctg
     3121 ctggtgtccg ccctgctcta tgccgtgcag ctctacgccg atttttccgg ctattcggac
     3181 atcgcgctgg gcgcggcccg ctgcctgggg ttcaagctgc ccgacaactt tcgccgccct
     3241 tacggcgccc gctccatcgg cgagttctgg gaccgctggc acatctcgct ctcgagctgg
     3301 ctgcgggatt atctctacat ccccctgggc ggcagccgca ggggcgtggc gcgcacctgc
     3361 gccaacaccc tgatcgtctt ttgggtcagc ggcctgtggc acggcgccgg gctgaccttt
     3421 ctcgtctggg gcggcctgca cggcctgtac aatgtggtct cacggctcac aaagcccctg
     3481 cgcctcgccg tcaaccgcct cacccggcta aaccgctgca aaaagctgca ccattttttg
     3541 cagcgggcga tgaccttttg catggtggcc tttgcctgga tctttttccg ggccgacagc
     3601 ctggatcagg cgctgctctt tatctcccgc ctgcccctgc ggctggggca gaccctgctc
     3661 agctggacgg cgataaaccg cgccctcgcc gggatcggct tttacgccaa caacggggtg
     3721 gctctgctct gcgccatcct gctgatgttc gcggccgagg cgctggagcg ccgccgcccg
     3781 ctggccgaga cggtggggcg ccagcccttt ctgctgcgct gggcgggcta ctacgcgctg
     3841 ctgctgggca tcctcttttt cggcgtgttc agcgccaccc cctttatcta cgggcagttt
     3901 taaagctgtc gaaaattgat gtttgtagaa caaaagcccc ccttttttca aaaagggggg
     3961 cttttttgcc gggttaagac gccttttgcc gcttttgcgc ccctcacgct cccccgcccg
     4021 gagcaggggt gtcctcacgc cctgcgcccg c
//
LOCUS       DBMDYA010000189         4031 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5236_length_4031_cov_1.585751, whole genome shotgun sequence.
ACCESSION   DBMDYA010000189 DBMDYA010000000
VERSION     DBMDYA010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_4031_cov_1.585751"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="ACZPFK_05860"
     CDS             <1..834
                     /locus_tag="ACZPFK_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013775.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR1405869.1"
                     /translation="FLRVHQMGGITNENFYLATAEEEIMLRLPGKCTETLDRPQEEKN
                     AAAAARAGLNPSTLYFSRETGVKITRYIRGAVTLDAALARKPEIMRKSAALLRRLHDT
                     LPPFENVFDPFALYDAYLARNADEFAGVFAGFEASRRVFAALRPALEALGLRSCPCHN
                     DTLADNLVMERDGGLYLIDWEYSGQNDPMWDVAGHMLESGFSDAQQEQFLTLYLGAPP
                     TRRDRQKVLLFQLCQDLLWSAQTMAKTLAGENQYEYALGRYERAQGLLREYRSRYGEL
                     G"
     gene            complement(1128..2216)
                     /gene="hemW"
                     /locus_tag="ACZPFK_05865"
     CDS             complement(1128..2216)
                     /gene="hemW"
                     /locus_tag="ACZPFK_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1405870.1"
                     /translation="MAFGLYLHIPYCEGKCFYCDFYSKGGSRSVPESYIEALLREMRR
                     FCNCECTSLRPDTFYFGGGTPSLLSPAQVARLISEAAPAPGAEITLEANPDTVTLDSL
                     RGYRRAGVNRLSIGVQSASDEQLRRLGRPHNAEAARRALELAREAGFGNISGDVMLAL
                     PHYSREEFDATLELLISGGATHISCYLLKIEPGTRFGLHPPQGLPGEDEAADFYLYAV
                     EQLARAGFAQYEISNFARPGYESRHNLIYWNCEDYLGLGPAAYSCINGERFHFPPDLN
                     AFLARIAPVAMDGRVDAEDYIMLQTRLAGGLSLPELRRRWGLELDGRQKSMLAACQQA
                     GLCHLTDDRLTLTPRGMLVQNSILTEVF"
     gene            2781..3179
                     /locus_tag="ACZPFK_05870"
     CDS             2781..3179
                     /locus_tag="ACZPFK_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405871.1"
                     /translation="MTIQEYIDKVAEQLELPEKVIQRIRKDLENELYEFVQGGADQAG
                     AIEKMGPVERTAAKLNEKYADTRGKEPLKKGEMPLLLPWCLIFSSALLLVKGAQVLFW
                     QGTGFDAALAVLGLALTAVSALAVLRYKKK"
     gene            complement(3722..>4031)
                     /locus_tag="ACZPFK_05875"
     CDS             complement(3722..>4031)
                     /locus_tag="ACZPFK_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase family"
                     /protein_id="HIR1405872.1"
                     /translation="GGEYLPYLRAVNGPITDTQISNMYFNRVFLGANAYSLDENSAYV
                     YDIREAYVKQLVKKRSEKVYFLAETSKCSQVDFYRAFSLDGCVLINEYTSEEEIADPV
                     "
BASE COUNT          794 a         1181 c         1301 g          755 t
ORIGIN      
        1 tttttgcggg tgcaccaaat ggggggcatc accaacgaga atttttatct cgccacagcg
       61 gaggaagaga tcatgctgcg cctgccgggc aagtgcaccg agacgctgga ccgcccgcag
      121 gaagaaaaga acgccgccgc tgcggcgcgg gccggtctca accccagtac cctctatttt
      181 tcccgcgaga cgggggtgaa gatcacccgt tacattcgcg gcgcggtgac gttggatgcg
      241 gcgttggcgc gcaagccgga gatcatgcgc aaatccgcgg ccctgctgcg ccgcctgcac
      301 gatacgctgc cgcctttcga aaacgtcttt gacccctttg cgctctacga cgcctatctt
      361 gcgcggaatg cggacgaatt tgccggggtc tttgccggat ttgaggcgtc gcggcgggtt
      421 tttgccgccc tgcgcccggc gctggaggcg ctgggtctgc gcagctgccc ctgccacaac
      481 gacaccttgg ccgacaatct ggtgatggag cgggacggcg gcctgtatct gattgattgg
      541 gagtacagcg gccagaacga tcctatgtgg gacgtggcgg ggcacatgct ggaatccggc
      601 ttcagcgacg cgcagcagga gcagttcctc accctctacc tcggcgcgcc gccgacgcgg
      661 cgcgaccggc aaaaagtgct gctgttccag ctctgccagg acctgctctg gtctgcgcag
      721 acgatggcga agaccttggc gggcgaaaac cagtacgagt acgcgctggg ccgctatgag
      781 cgtgcccagg gcctgctgcg ggagtatcgg agccgatacg gcgagctggg ataaccggag
      841 aaataaccgc tgtgcggcta ttttttcggt cgtgcagccg gtccaccccg ccggagccat
      901 caagataagt tgctttttat tgcggagcaa taaaaatggc cgaagcgcag cggagactgt
      961 tttttcggcc gtccgtctcg attgccgcgc agcggcgaaa agcggcgcat cttgagagaa
     1021 aagagttttg atgcggcggc aaccaaaagc ccgccggcgc gctccggcgg ccggcgggca
     1081 ggtttttatg ggggaatgat ccgtgccgcg cccatcgacg cgacggctca aaacacctcg
     1141 gtgagaatcg aattctgcac cagcatcccg cggggggtga gggtgagccg gtcatcggtg
     1201 agatggcata gccctgcctg ctggcaggcg gcaagcatgc ttttttggcg cccatccaac
     1261 tccaggcccc agcggcggcg cagctcgggc aaagagaggc cgccggcaag ccgggtctgc
     1321 agcatgatat agtcctctgc atctacgcgc ccgtccatcg ccaccggggc tatacgggcc
     1381 aaaaaggcgt tcaggtccgg cgggaagtgg aatctctcgc cgtttatgca ggagtaggcg
     1441 gcgggcccga gaccgagata gtcctcgcag ttccagtaga tcaggttatg gcggctctca
     1501 tagccggggc gggcaaagtt gctgatctcg tattgggcga atccggcccg ggccagctgt
     1561 tccacagcat agaggtaaaa gtctgcggcc tcgtcctcgc cgggcaggcc ctgcggcgga
     1621 tgcaggccga aacgggtccc aggctcgatt ttcagcagat agcagctgat atgggtggct
     1681 ccgccgctga taagcagctc cagcgtggcg tcgaattcct cccggctgta atggggcagg
     1741 gccagcatca cgtcgccgga gatgttcccg aaaccggctt cccgggccag ctccagcgcc
     1801 cggcgcgccg cctcggcgtt gtgaggccgg cccaggcggc gcagctgctc atccgacgcg
     1861 ctctgcaccc cgatggagag ccggttcaca ccggcccgcc gatacccgcg caggctgtcc
     1921 agcgtgaccg tgtcggggtt ggcctccagc gtgatctccg ccccaggggc aggggcggcc
     1981 tcgctgatga gccgggcgac ctgagccggg ctgagcaagc tgggagtgcc gccgccaaag
     2041 tagaaagtgt ccggccgcag gctggtgcat tcgcagttgc aaaagcggcg catctcccgc
     2101 agcagcgcct caatatagct ctccggcacc gagcggctgc cccccttgga gtagaagtcg
     2161 caataaaaac acttcccctc acagtagggg atgtggagat agagcccaaa cgccatgagg
     2221 aacctccttg tgtcctggaa ttctagtgta tagcgcgctg tgcggttttg cacgctccct
     2281 tttccggctg ggaaaacgcg gcctgggcgc gggccattct cttcaaagcg gaccggttgt
     2341 acccgttccg cctttttcgc tcggcgcaaa gtggccgcga ttttttcttc ccggccggga
     2401 gactggaaca aaacgagtga aaaaggtatc tgtgacggag aggagcggga gagcagaagc
     2461 tgtcccgccc gttgccgccc ctcgggcaaa gcgggactga aaaaacggcc ctcgccattg
     2521 ctccccggaa ctgcccgcgg ccgtgctgct gctttttgcg cgcatccgcc tgttgtgcgg
     2581 cagaagctca agctcgcagc gccgcggcca agaggcacac gggcgtgaaa ggtgcggcgt
     2641 ctttgtggaa cgatcggcgc agccgcgcaa aagcggatgt tgtatcgatt gccatttttt
     2701 atgacctgag agggagcggc ggtatttgag aaaagagatg aaaactgcta tactgtcttc
     2761 tgaagttgga gggatagggt atgacgatcc aggaatatat cgacaaagtg gccgagcagc
     2821 tggagctgcc agaaaaggtg atccagcgca tccgcaagga tctggaaaac gagctgtatg
     2881 aatttgtgca gggcggcgcg gaccaggcgg gcgccatcga aaaaatgggc ccggtggagc
     2941 ggaccgccgc caagctgaac gaaaaatacg cggatacccg cggcaaagag ccgctcaaga
     3001 aaggggagat gccgctgctg ctgccctggt gcctgatctt ttcttcggcg cttttgctgg
     3061 tgaaaggggc ccaggtgctg ttttggcagg ggacggggtt tgacgcggcg ctggcagtgc
     3121 tggggctggc cctgaccgct gtgtccgcgc tggcggtgct gaggtataaa aagaagtaat
     3181 aaaaatggat ggcggtcgcc atccattttt tattgcttcg caataaaaag cttatcccaa
     3241 tgcacctgtg cctcgcccct ggcggggcaa ccggcccaga ggtgccaaaa atagccttac
     3301 gactattttt ggcggctccg caacaaaaga caaattatcc tgatggagcc gttgctcgcc
     3361 gctggcgcgg caagcgaaac gaatagccgt cggctgtttt ctctcgcttc cgtcccggcc
     3421 gcagagattc gattcctctg ccgcaatgcc gccgcgccgc ctccctgggc caaaggggac
     3481 ggtgcgccag gggggaggct tttcgcgctt tttgctttcg caggatagag gacttcccct
     3541 tttttgcaga gctttctcac tgagtgcagc ccaaaacagt cgccccatgg ccccttttgc
     3601 ggggctgccg cgccgtacct gggaggttct ccgcagcgga gcgcggctgc cgccaaaggg
     3661 gcaaacagac cagagaagcg aaaagtgacg gaccgacccg ggaaccagag aaaaaagccg
     3721 gtcagaccgg gtcggcgatc tcctcctccg aggtgtactc gttgatcagg acacagccgt
     3781 ccaggctgaa agcgcggtaa aaatccacct ggctgcactt gctggtctcg gccaggaaat
     3841 agaccttttc cgagcgcttt ttgaccagct gcttgacgta agcctcgcgg atatcataga
     3901 cataggcgct gttttcatcc agagaatagg cgttcgcgcc gagaaagacg cggttaaaat
     3961 acatattgga gatctgggta tcggtgatgg gcccgttcac cgcgcgcagg tagggcagat
     4021 actccccgcc c
//
LOCUS       DBMDYA010000190         4027 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5244_length_4027_cov_1.415690, whole genome shotgun sequence.
ACCESSION   DBMDYA010000190 DBMDYA010000000
VERSION     DBMDYA010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_4027_cov_1.415690"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="ACZPFK_05880"
     CDS             <1..539
                     /locus_tag="ACZPFK_05880"
                     /inference="COORDINATES: protein motif:HMM:NF022635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HIR1405873.1"
                     /translation="RKVTAQQGYRSIAPRVRTLVPQSSVVGMLLEHEEDYTVVHSTQL
                     GLMQHDLYSWQVRRAGFVCLESVDEGSRIVDRALKEWIASMTPAQRETFVDVLFGLLD
                     ASGAQTVGQLSLGWLKNAGALARALKGIDEPTREAMSAILKALSHAARHSLAQAFPRG
                     EERRQSVCPAPRGPGAPN"
     gene            649..1962
                     /locus_tag="ACZPFK_05885"
     CDS             649..1962
                     /locus_tag="ACZPFK_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1405874.1"
                     /translation="MAWYEKSVFYQIYPLGFCGAPWQNDGVTEDRILKVMDWIPHLTA
                     LGADAVYFSPVFESDTHGYNTRDYARVDCRLGGNAAFAKVCAALHAAGVRVVLDGVFN
                     HVGRGFWAFQDVLQHRQASRYAGWFHLSFEGNSAYNDGLWYEGWEGNYDLVKLNLDNP
                     EVVEYLLDCVGGWISEFDIDGLRLDVAYLLPEHFLRRLRAFCDGQKAEFFLVGEMIHG
                     DYNRLVQPDMLHSATNYECYKGLHSSFNSGNLFEIAHSLARQFGPENWTLYRGKHLFN
                     FVDNHDVSRIASLLSDKKQLPCIYGLLFAMPGIPCLYYGSEWGAEGEKRPGGDENLRP
                     SFDAPQKNQLTDFLARLSAAHKGSAALTCGDYKNIQIAPKQLIFQRQAEGERVLVAVN
                     ADGAPVTMHFDAGAGRATELITGETHDFGGGSELPPYSVAYWRVE"
     gene            complement(2056..2898)
                     /locus_tag="ACZPFK_05890"
     CDS             complement(2056..2898)
                     /locus_tag="ACZPFK_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1405875.1"
                     /translation="MSEGCTHNCETCSEACASREGGAPQDLHEAANPNSNIKKVIGVV
                     SGKGGVGKSLVCSMLAVEMRRRDYSTAILDADITGPSISKMFGLKTRAVGSELGILPV
                     ETRTGVKVMSVNLLLEHETDPVVWRGPVIAGTVTQFWTDVVWGDVDYMFVDMPPGTGD
                     VALTVFQSLPVDGIIIVASPQDLVSMIVEKAVKMAGLMNVPILGLVENMSYFTCPDCG
                     KKISVFGESHIDEIAASYNVPVLGKLPIDPGLAKQCDMGVIEAFEGNGLADGVEALEN
                     LLKK"
     gene            3159..3308
                     /locus_tag="ACZPFK_05895"
     CDS             3159..3308
                     /locus_tag="ACZPFK_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405876.1"
                     /translation="MKQEKKTIAQILLRRPLRGCEAKEKMQRENPVYHDCYGTKIATK
                     QVAGR"
     gene            3354..3926
                     /locus_tag="ACZPFK_05900"
     CDS             3354..3926
                     /locus_tag="ACZPFK_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1405877.1"
                     /translation="MLPVMVFDTPEQKSKFEEAYLKYNKMGFYYAREILHDDALAEDA
                     LHDAFLSLARNLDKIGEIDCNKSFNYFVTIVKNTAISALRKHRRAQVVEIDDEDTLFQ
                     LEDSAPGPEETVLRQDSYEALLAAIRTLKPEYQAPLLLKYANGYTEAEIAKALGMTVS
                     NVSVRIHRAKQKLIPMLRERGGTGLAQTTE"
BASE COUNT          785 a         1198 c         1311 g          733 t
ORIGIN      
        1 agcggaaggt caccgcacag cagggctacc ggagcatcgc accccgggtg cgcaccctgg
       61 tgccgcagtc ctcggtggtg ggcatgctgt tagagcacga ggaggactac accgtggtgc
      121 acagcaccca gctggggctg atgcagcacg acctttactc ctggcaggtg cgccgcgccg
      181 gcttcgtctg cctggagagt gtggacgagg gcagccgcat cgtggaccgg gcgctgaaag
      241 agtggatcgc ctcgatgacc ccggcccagc gcgagacctt tgtggacgtg ctgttcggcc
      301 tgctggatgc cagcggggcc cagacggtgg gccagctcag cctgggctgg ctcaaaaacg
      361 cgggcgctct ggcgcgggcg ctcaagggga tcgacgagcc cacccgggag gccatgtccg
      421 ccattttgaa agcgctttcc cacgcggcgc gccacagcct tgcacaggct tttccccgcg
      481 gggaagagcg gcggcagagc gtctgtccgg ccccgcgcgg gcccggcgcg ccaaactgac
      541 attttgcccc gtctccgcct ggaggaatga gaatggggta aaatgtggta tactgaagaa
      601 caagaaaaca gcggcccgtc tctccgggcc ccggaaatga ggaatttcat ggcctggtac
      661 gaaaagagcg ttttttatca gatctatccg cttgggttct gcggcgcgcc ctggcagaac
      721 gacggcgtga ccgaggaccg catcctgaaa gtgatggact ggatccccca cctgaccgcg
      781 ctgggcgcgg acgcggtcta tttttctccg gtgttcgaga gcgacaccca cggctacaac
      841 acgcgggact atgcccgggt cgattgccgc ctgggaggca acgccgcctt tgccaaggtc
      901 tgcgcggcgc tgcacgcggc cggtgtgcgg gtggtgctgg acggcgtgtt caaccacgtg
      961 ggccggggct tttgggcctt tcaggatgtg ctgcagcacc ggcaggcctc ccgctacgcg
     1021 ggctggttcc atctcagctt tgagggcaac agcgcctata acgacggcct ctggtacgag
     1081 ggatgggagg gcaactacga tctggtcaag ctgaacctcg acaacccgga ggtggtggag
     1141 tatctgctgg actgtgtggg cggctggatc agcgaatttg atatcgacgg gctgcgcctg
     1201 gatgtggcct atctgctgcc ggagcatttt ctgcgccgcc tgcgggcgtt ctgcgacggc
     1261 caaaaggcgg agttctttct ggtgggggag atgatccatg gagattacaa ccggctggtg
     1321 cagccggata tgctgcactc ggccaccaat tatgagtgct ataaggggct gcattccagc
     1381 ttcaacagcg gcaacctgtt tgagatcgcc cattcgctgg cgcggcagtt cgggccggag
     1441 aattggaccc tctaccgcgg caagcatctg ttcaactttg tggacaatca cgacgtgtcc
     1501 cgcatcgcca gccttttgag cgataaaaag cagctcccct gcatctatgg cctgctcttt
     1561 gccatgcccg gcatcccctg cctttactac ggcagcgagt ggggggccga gggggaaaaa
     1621 cgcccgggtg gggacgaaaa cctgcgcccc tcttttgacg cgccgcaaaa aaaccagctc
     1681 accgattttc tggcccggct cagcgcggcc cacaagggca gcgccgcgct cacctgcggc
     1741 gactataaaa atatccagat cgcgccaaaa cagctcatct tccagcgcca ggcggagggg
     1801 gagcgggtgc tggtggccgt gaatgccgac ggcgcgccgg tgacgatgca ttttgacgcg
     1861 ggcgcaggcc gcgcgaccga gctgatcaca ggcgaaacgc acgattttgg cggcggcagc
     1921 gagctgccgc cctacagcgt ggcctattgg cgcgtggagt aaagcgccgt ccggctatgg
     1981 gcggcggggt ggcataaaca gaaagagacg gagaccggtt ttgcccggcc gccgtctctt
     2041 ttttatgcgg agcccttatt ttttcagcag gttttccagc gcctcgacgc cgtcggccag
     2101 cccgttgccc tcaaaggcct cgatcacgcc catgtcgcac tgtttggcaa ggcccgggtc
     2161 gatgggcagc ttgcccagca ccgggacgtt gtaggaggct gcgatctcgt cgatgtggga
     2221 ttcgccgaag acagaaatct ttttgccgca gtcggggcag gtgaaatagc tcatgttttc
     2281 caccaggccc aggatgggca cgttcatcag cccggccatc ttgaccgcct tttctacgat
     2341 catggacacc aggtcctgcg ggcttgccac gatgatgatg ccgtccaccg gcagggactg
     2401 aaaaacggtc agagccacgt cgccggtgcc cgggggcatg tccacaaaca tgtaatccac
     2461 gtcgccccag accacatcgg tccagaactg ggtcacagtg cccgcgatca ccgggccgcg
     2521 ccacaccacc gggtcggtct cgtgctccag cagcagattg accgacatca ctttcacgcc
     2581 ggtgcgggtc tcgacgggca gaatgcccag ctcgctgccg accgcccggg tcttcagccc
     2641 gaacatcttg gagatggagg ggccggtgat gtccgcgtcc aggatcgcag tgctgtagtc
     2701 gcggcggcgc atctccaccg ccagcatact gcagaccagg ctcttgccga cgccgccctt
     2761 gccgctgacg acgccgatca cctttttgat gttgctgttg ggatttgccg cctcgtgcag
     2821 gtcctgcggc gccccgccct cgcgggaggc gcaggcttcg ctgcaggtct cacagttgtg
     2881 ggtgcagcct tcgctcatga aaacgatacc tcttttctgt atagtgacag agctttacag
     2941 gcgtgtgccg aggctctgta taggtccgcc acgcggacgt tgtgccttgt cagtatacca
     3001 cgaacgggcg cgcatttcaa cgccgccgcg gttgcggcag gtcttgattt atgctataat
     3061 gacgcaaaat gccatggaaa tgcgggggat cgggccgctt gaaaagcagc cgcgggcgcg
     3121 gcccgaggcc ggcccccgcg ccgcggggag gcggatggat gaaacaggaa aaaaagacca
     3181 ttgcgcagat cctgttgcgc cggccgctgc gcggctgcga ggcgaaagag aagatgcaga
     3241 gggagaatcc cgtctatcac gactgttacg ggacaaagat tgccacgaaa caggtcgctg
     3301 gccgctgagg cgcggcagag ccgggtgaac aggcgcgaga ggaggggcgg gcagtgctgc
     3361 cagtcatggt attcgatacg ccggaacaga agagcaagtt tgaagaggcc tatctcaagt
     3421 ataacaagat gggtttttac tatgcccggg aaattttaca cgacgacgcc ctggcggagg
     3481 atgcgctgca tgacgccttt ctttcccttg cacgcaatct ggacaagatt ggagaaatag
     3541 attgtaacaa atcctttaat tatttcgtta ctatcgtgaa aaacaccgcg atctcggcgc
     3601 tgcgcaagca caggcgggcg caggtggtgg agattgatga cgaggacacg ctttttcagc
     3661 tggaggactc ggcccccggg ccggaggaga cggtactgcg ccaggacagc tacgaggcgc
     3721 tgctcgccgc catccgcacc ctcaagccgg agtaccaggc gccgctgctg ctgaaatacg
     3781 ccaacggcta cacggaggcg gagatcgcga aagcgctggg catgacggtg tccaatgtct
     3841 cggtgcgcat ccaccgggca aaacaaaagc tgatccccat gctgagagag agaggaggta
     3901 ccggccttgc acagaccaca gaatgacggc gcagccgatt ttgaggcgct gctcgcgcgg
     3961 gcggccagcc agatcgccgc cgagcaggcg gacgaggtgc gccaaaagta tgaaaacgcg
     4021 cccgagg
//
LOCUS       DBMDYA010000191         3998 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5286_length_3998_cov_1.454965, whole genome shotgun sequence.
ACCESSION   DBMDYA010000191 DBMDYA010000000
VERSION     DBMDYA010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5286_length_3998_cov_1.454965"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(403..1143)
                     /locus_tag="ACZPFK_05905"
     CDS             complement(403..1143)
                     /locus_tag="ACZPFK_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1405878.1"
                     /translation="MNVIVTESYEASAAWVAEQICALIVAKPDAKLGLATGGTPVPVY
                     RALIEACKEGRADFSAVHTVNLDEYWGLKPDNDQSYRYFMDQNLFDHINIKKENTYVA
                     SGLGDPEENCRALDAAVMEGGAPDLQLLGIGGNGHIAFNEANPDRLIAPSHLEDLTPG
                     TIQANSRFFDRPEDVPTKAITMGMAGIMAAKSVVLLATGASKVEAIRGLVMEDAVTTR
                     NPSTLLKLHPNATVVIDRALADAVGYQG"
     gene            complement(1527..1919)
                     /locus_tag="ACZPFK_05910"
     CDS             complement(1527..1919)
                     /locus_tag="ACZPFK_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017416.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1405879.1"
                     /translation="MYQHSLDQLSVYDVVSPLGDAVRPDNRWVRLAARIDWRALEEQY
                     AAHFKRGGKQAIPLRCAFGSLVIKRALGVSDRQTVELIRESPYLQYFIGLPVFTETPP
                     FSARSMAAFRARIPEREVARAARLLHKK"
     gene            2644..3024
                     /gene="gloA2"
                     /locus_tag="ACZPFK_05915"
     CDS             2644..3024
                     /gene="gloA2"
                     /locus_tag="ACZPFK_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIR1405880.1"
                     /translation="MSLSAVHHVAVLVKDYQKSRHFYVDLLGFPVVRENYRPQRGDWK
                     LDLAVGELELEIFSAPAAPDRPTRPEALGLRHLAFRVESVEREAAALNAKGIATEPVR
                     VDEFSGKPFTFFFDPDGLPLELHE"
     gene            complement(3558..>3998)
                     /locus_tag="ACZPFK_05920"
     CDS             complement(3558..>3998)
                     /locus_tag="ACZPFK_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1405881.1"
                     /translation="LCQALEAGDPLPFDPRDAVVYYVGPTPARPGHVIGSAGPTTSYR
                     MDAYSPALLGQGLRGMVGKGLRGPAVVEAMRRHHAVYFGAVGGTGALIGRSIKTAEIV
                     AYEDLGTEAIRRLTVEQLPLTVVIDTLGNNLYELGPRRYLESLG"
BASE COUNT          733 a         1201 c         1349 g          715 t
ORIGIN      
        1 ctttcccacc cctctataaa aaggaaaaac agccgccatt cccgggcggc tgtttttctt
       61 tgtttcaaac agatgccgcc gcgtggggga gaatcccggg atgtcggaga aaaaacgggg
      121 cacagcgctt tggacgttcc gctcccgctg tctgcggcgg ccggggccgg gcaccgcgaa
      181 aaagccgctc ttcccacggg gggctaaagg cgtctatggc aaagatggcg gggatgcgcc
      241 ccgggggcgc gcggccgttc aggcggcggg ctggtctctg gagccgctgc ttcccacgtc
      301 ctgggcggag ggggagaaac cgcgcaaaaa aggccgggag ggcgctggat cgcgcggtct
      361 cccggccttt taatacccat ctggggtgtc aggggctttc tctcagccct gatagcccac
      421 ggcgtcggcc agcgcccggt cgatgacgac ggtggcgttg gggtggagct tgagcagggt
      481 gctggggttg cgggtggtga ccgcgtcctc catcaccagg ccgcggatcg cctccacctt
      541 ggaagcgccg gtagccagca gcaccaccga tttcgcggcc atgatgcccg ccatgcccat
      601 ggtgatggcc ttggtgggca catcctcggg ccggtcgaaa aagcggctgt tggcctggat
      661 ggtgccgggg gtcaggtcct ccaggtggga gggggcgatg agccggtcgg ggttggcctc
      721 attgaaagcg atgtggccgt tgccgccgat gcccagcagc tgcaggtcgg gcgcgccgcc
      781 ctccatcacc gcggcgtcca gcgcccggca gttttcctcc gggtcgccca ggccgctggc
      841 gacatatgta ttttcttttt tgatgttgat gtggtcaaag aggttctgat ccataaaata
      901 gcgatagctt tggtcgttgt cgggcttcag gccccagtat tcgtccaggt tgacggtgtg
      961 cacagcggaa aaatccgccc ggccctcttt gcaggcctcg atcagggcgc ggtagaccgg
     1021 cacgggggtg cccccggtgg cgaggccaag ctttgcgtcg ggcttggcga cgatcagcgc
     1081 gcagatctgt tctgccaccc acgcggcgga ggcctcatag ctttcggtga cgatcacatt
     1141 catgctgttc ctacactcct ttgcaggccc ggggaaaggg ccttcttcac ttgaaaacat
     1201 tgtaccgcat ttgaaaaaaa gttcacaggg ggaaaaggga ttatttttgt cgcttggaca
     1261 ggcaggaggc acaaaaaaga gaaaacagcg cccggcttgt cctttttggg gagaaccgga
     1321 cgcgcaggga gaggggatcg tccgccccct gtttttgtgg ttttgcagtc agagagaggc
     1381 ggcgcggccc cgccgagggc gcaaaagacc gaaaaaagga accgcggatt gtaaaaaccg
     1441 ctgtatgccg ccccgggcca cgggcttaaa gtcttgcgag gccgcccaca aagcggcgga
     1501 catacctaaa aacagccgcc gcgcggctat tttttgtgca gcagccgggc ggcgcgggcg
     1561 acctcccgct cggggatgcg ggcgcgaaag gcagccatgc tgcgggcgga gaacgggggc
     1621 gtttcggtga agaccggcag cccgataaaa tattgcagat aggggctctc gcggatcagc
     1681 tccaccgtct ggcggtccga gacgcccaag gcccgcttga tcaccaggct cccgaaagcg
     1741 cagcgcagcg ggatggcctg cttgccgccc cgcttgaaat gggcggcata ctgctcttcc
     1801 agcgcgcgcc aatcgatgcg ggcggccaga cgcacccagc ggttgtcggg ccggacggcg
     1861 tcccccagcg gggagacgac gtcgtacact gaaagctggt ccagggaatg ctgatacatt
     1921 ttgcccacac cctccgcaat ggtcttttgg tgctgcgcac agttgaaccg gacggtccca
     1981 cctcctcgcc gccgtgcggc gaccgggccc ggaggcggaa aagtattgct ccgccgcaaa
     2041 aataattgcc cgtgacggct ccggccgggg aagctggacg gatgatctga aacacatttt
     2101 cattttttat tttttgccgg gggcaatcgc gcccaacacg gttcggccgc tgtgcggcgc
     2161 catggaaagg ggcctcgccg cacaagggcc gcagccgttt gccgcaaaaa gctatcgtcg
     2221 aattattatt tttattatag cacaaaaaga tggtgaaaac cacttgtccg cgggagaaaa
     2281 acaatatgac cgtccaaccc gacaccggtc aggcgcgcct gggtgcgcct gaaagaaaag
     2341 aagcccggca tgcgcgggat cgccgttcat gatataatgt ctgcgggaga cttgtgacgc
     2401 cggtccgccc ccccgacgag cgcgggccgg ccgctgccgt ccgtccggcc ccgctccgcg
     2461 gaaaggtcct gcttgcggag aaaagccgtg ttccggcttt gccgggggcc ggtgcggctg
     2521 cgcgcaggca cggcgaaaag tcggcgaccg gccccgcgca gaaggccctg cttgcgggag
     2581 aagccgtgtt ccggctctgc cgggtggccg gcgcggagaa aagcaaaaga ggaggagacc
     2641 gttatgagct tgagcgcggt gcaccatgtg gctgttttgg taaaagatta tcagaagagc
     2701 cgccatttct acgtggatct gctgggattc ccggtggtgc gggagaatta ccgcccccag
     2761 cgcggggact ggaagctgga ccttgccgtg ggggagctgg agctggaaat tttcagtgcc
     2821 cccgcggcgc ccgaccgccc cacgcggccg gaggcgctgg ggctgcggca tctcgctttc
     2881 cgggtggaga gtgtagagcg ggaggccgcg gcgctgaacg ccaaggggat cgccaccgaa
     2941 ccggtgcggg tggacgagtt ttcgggcaag ccgttcacct ttttctttga cccggacggg
     3001 ctgccgctgg aattgcacga gtgaggagaa gagaacaaaa aaggcggaga gccgggcaca
     3061 gctatacggc tgtgctcggc tctccgcctt ttgtcttttg agcccggcgc tcgccgcctt
     3121 ttgaaacggc tccgccccca aagccgcatt tccacaagaa aatattgccc tctccaaaga
     3181 gcgagatttg cgcgcagatc gccgtcaggc tcaccccgag accatagagg cttcgcggcc
     3241 tcaccctgtg aaacgtttgc cccgacaggc aggcaaacgc cgccgcgggc ggcaggcgcg
     3301 ggttttctca gctccgcatc gcagctttcc ctctcagagg tgtgcggcgg tttgaagaga
     3361 gcacaataca aaacaaggcc ggctgtctct gcagattgcc gacctttttg aacctgtttt
     3421 tgagtgtgcc ggggcgtcaa gggcgccgtt cccaaaccga tgttttctca ttttgaagtg
     3481 aggagaagag gcgggcgctt ttggggccgc ggcggccggg ttttccagat cggctgccaa
     3541 aaaggccgca ggccgcatta ccccaggctc tccagatagc gccgggggcc cagctcatac
     3601 aggttgttgc ccagcgtgtc gatgaccacg gtcagcggca actgctccac ggtcaggcgg
     3661 cgtatcgcct cggtgcccag gtcctcatag gcgacgatct cggcggtctt gatggagcgg
     3721 ccgatcaaag cgccggtgcc gcccaccgcg ccgaaataga cggcgtggtg gcggcgcatg
     3781 gcctccacga cggccgggcc gcgcagcccc ttgcccacca tgccgcgcag gccctgcccg
     3841 agcagcgccg gggagtaagc gtccatgcgg tagctggtgg tgggcccggc gctgccgatg
     3901 acatgtccgg ggcgggccgg ggtgggcccc acgtaataga cgacggcgtc ccgcgggtca
     3961 aaggggaggg ggtcccccgc ctccagcgcc tggcagag
//
LOCUS       DBMDYA010000192         3994 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5293_length_3994_cov_1.515284, whole genome shotgun sequence.
ACCESSION   DBMDYA010000192 DBMDYA010000000
VERSION     DBMDYA010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5293_length_3994_cov_1.515284"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(294..923)
                     /locus_tag="ACZPFK_05925"
     CDS             complement(294..923)
                     /locus_tag="ACZPFK_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1405882.1"
                     /translation="MKQLADFIKSAPQPVARGFASVRFGAGEHILWEGEENRWVYLLL
                     EGSAEGYLQSAQGGVSSVFYYQPVSLFGEFEVFAPGGNIINVAALSPCRLLRLSGELF
                     LEWIKSDFEFNCHVIRQIVEKTAQNAIALDCFSHSTAREKVLRCAAAWHSGGRLSRLT
                     KEALCAETNLPLRSLNRALAVCAEEGVLRYEKKHFRVLDEQRLLAYLPR"
     assembly_gap    975..984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1260..2219
                     /locus_tag="ACZPFK_05930"
     CDS             1260..2219
                     /locus_tag="ACZPFK_05930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405883.1"
                     /translation="MRHFLIDTDTAGDDAIALMMLLQEPQVRVEAVTTVAGNVPMEQA
                     TKNALATIEVTGSYQPPVYRGAARPLMRDLAQMIGVHGKDGMGDCDLIAPTLSPAPGH
                     AVDAICELAKRYPDELEILMIGPATNVALALMKEPEVMRRVKHIYSMGTGGFALGMET
                     AVAEFNVYIDAEAYAAVLDSGIPITIAGFDICMGETALNEEEAVLLARRGTPAGKFAA
                     ACNAKLLEFARRRLGVGMIPLPDPTAAAAAIWPEVVLETEDRYCYTCTKEEATYGQVI
                     PFVPDAEIKDQGFHRRAANARLIKKLDAARFKEKLLSLLLAAE"
     gene            complement(2305..3438)
                     /locus_tag="ACZPFK_05935"
     CDS             complement(2305..3438)
                     /locus_tag="ACZPFK_05935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405884.1"
                     /translation="MKRKFSDRFPSLARALAVLAGVLLVTGGLVYLVDEALNGFVADL
                     FYNTFIWTRQDPVSGALIREPYWPALKSFLIVAFLLAALVVALVVFLAAHFYGKRRAA
                     REVAFLSDCLRRYFAPGGSRLALPAAYAEFENQMRVVDEDIRLREEAMARQTAEKNDL
                     ITYLAHDLKTPLASVIGYLSLLDESPDLPAPQRRKYVRVTLDRANRLEQLVEEFFDIT
                     RFNLHAIALTRTRLDLPLLLRQLADEFYPLLAPAGKRVTVDAEEGLTLRADPDKLARV
                     FNNLLKNAAAYSDPGTEIALCARREQNRAVVTVTDTGAEIPPQKLEMIFEKFYRLDSS
                     RSTGTGGAGLGLSIARQIVEAHGGELAAESRGGVTVFTVRLPL"
     gene            complement(3428..>3994)
                     /locus_tag="ACZPFK_05940"
     CDS             complement(3428..>3994)
                     /locus_tag="ACZPFK_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1405885.1"
                     /translation="GPFDLAVLDVMLPDTSGFSLCQKIREQYNYPVLLLTAREGELDK
                     INGLALGADDYVTKPFRPLELIARVKAQLRRYTKYNPAEAARAEETLAFRGLTLDRAT
                     HECTLDGRIVPLTPTEFSILWILAENRGRVVSSEELFRQAWGEKYFRNENNTVMVHIR
                     HLREKLGEGAEKPRYIRTVWGVGYKIEA"
BASE COUNT          711 a         1271 c         1327 g          675 t
ORIGIN      
        1 cacccctggc acggttccgc tggtaggggc cgccgcccgc tcccagcgga agaagtcggt
       61 ccgccgcatc ggcgccgctt gtaggctgca aaggcctgcg gcgcgccgct tttttgcgat
      121 ggggcctttt ccgcgaacgg cccatgccgc gcagctttgt cacagttgcg caaactcgcc
      181 tttgccccgc agccccgctc ttgctgcgcg gcgtggtccg gcggggcaaa accaaatgtt
      241 ccctgccgtc agttcgcttt gaggatgcgc ttttctcccg cgccgcctct gtctcagcgg
      301 ggcagatagg cgagcaggcg ctgttcgtcc agcacccgaa aatgcttttt ttcatagcgc
      361 agcacgccct cttcggcaca gactgccaag gcccggttca ggctgcgcag cggcaggttg
      421 gtctcggcgc acagcgcctc cttggtgagc cgggacaggc ggccgccgct gtgccaggcc
      481 gcggcgcagc gcaggacctt ttcccgcgcg gtggagtggg aaaaacaatc cagcgcgatg
      541 gcgttttgag ccgtcttttc cacgatctgg cggatgacat ggcagttgaa ttcaaaatcg
      601 ctcttgatcc actccaaaaa gagctccccg gacagccgca gcaggcggca ggggctgagg
      661 gccgccacgt tgatgatatt cccccccggc gcgaacacct caaattcgcc gaagaggctg
      721 accggctggt aatagaagac cgagctgacg ccgccctggg cgctttgcag atatccctcg
      781 gcgctgccct ccagcagcag atagacccag cggttttcct ccccctccca caggatgtgc
      841 tccccggcgc caaagcgcac cgaggcaaag ccgcgggcga cgggctgcgg ggcgcttttt
      901 ataaagtcgg ccaactgttt cacgcgcggt ccctcccttt tgagataaaa agaatgcggc
      961 cggcggcccg cctgnnnnnn nnnngcggga aaaagccgtg cgtgggccgc ggcagcctgg
     1021 gggaaagccg tccgggctcc cgcccagggg gtgataaaaa atagccgcag gctatttttt
     1081 gcggccattc ggccagtcct ctggccgggg ccattaaaat aagccgtttt ttattgcaaa
     1141 gcaataaaaa acatattttg ggccaaccga cctggatttc agtatagcgt ttcccttata
     1201 ctgatgtcaa acaggtcccg gcggtccgcc gggagaacac gacgagggag ggctttttca
     1261 tgagacattt tctgatcgac accgacacgg ccggcgacga cgccattgcg ctgatgatgc
     1321 tgctgcagga gccgcaggtg cgggtggagg cggtgaccac cgtggcgggc aatgtgccga
     1381 tggagcaggc gacaaaaaac gcgctcgcca ccattgaggt caccggcagc taccagccgc
     1441 ccgtttatcg cggcgcggcg cggccgctga tgcgggacct cgcccaaatg atcggggttc
     1501 acggcaagga cggcatgggc gactgcgatc tcatcgcccc cactctgtcg cctgcgccgg
     1561 gccacgcggt ggacgcgatc tgcgagctgg caaagcgata cccggacgag ctggaaatcc
     1621 tcatgatcgg cccggcgacc aacgtggccc tggccctcat gaaagagccc gaggtcatga
     1681 ggcgagtaaa gcacatctac tcgatgggca ccggcggctt tgccctcggc atggagacgg
     1741 cggtggcgga gttcaacgtc tacatcgacg ccgaggccta cgctgcggtg ctggacagcg
     1801 gcatccccat caccatcgcc gggttcgaca tctgcatggg ggagaccgcc ctcaatgagg
     1861 aggaggcggt gctgctggcc cggcgcggca ccccggcggg caaattcgcg gcggcctgta
     1921 acgccaagct gctggagttt gcgcgtcggc ggctgggcgt gggcatgatc ccgctgccgg
     1981 accctacggc ggcggcggcc gccatctggc ccgaggtggt gttggagacc gaggaccgct
     2041 actgctatac ctgcaccaaa gaggaggcca cctatggaca ggtgatcccc tttgtgccgg
     2101 atgccgagat caaggaccag ggcttccacc gccgcgccgc caacgcccgg ctcatcaaaa
     2161 agctggacgc ggcccggttc aaagaaaagc tgctgtcact gctgctggcg gcggagtaaa
     2221 gaccggcccc acgcccgggg gacggagaaa aagacaaaaa aggaagcgcc gcttgcagcg
     2281 cttccttttt tgtcccgcgg ctgctcacag cggcagacgc accgtgaaaa cggtcacgcc
     2341 gcctcgactc tcggccgcca gctcgccgcc gtgggcctcc acgatctggc gggcgataga
     2401 gaggccgagc cccgccccgc cggtgccggt ggagcgggag ctgtccagcc ggtagaattt
     2461 ttcaaagatc atctccagct tttgcggcgg gatctcggcg ccggtgtcgg tgacggtgac
     2521 caccgcccgg ttctgctcgc ggcgcgcgca cagggcgatc tcggtgcccg ggtcgctgta
     2581 ggcggccgcg tttttgagca ggttgttgaa gacccgggcc agcttgtccg ggtcggcgcg
     2641 cagggtcagc ccctcctcgg cgtccaccgt cacccgcttg cccgcgggcg cgagcagcgg
     2701 gtagaattcg tccgccagct ggcgcagcag cagcggcagg tccagccggg tgcgggtgag
     2761 ggcgatggcg tgcaggttga agcgggtgat gtcgaagaat tcctccacca gctgttccag
     2821 ccggttggct cggtccagcg tgacgcgcac gtacttgcgc cgctggggcg cgggcaggtc
     2881 gggggattcg tccagcaggg acagatagcc gatcaccgag gcgagcggcg tcttcaggtc
     2941 gtgggccagg taggtgatca ggtcgttttt ctccgccgtc tgccgggcca tggcctcctc
     3001 ccgcaggcgg atgtcctcgt ccaccacccg catctggttt tcaaactcgg cataggcggc
     3061 gggcagagcc aggcgggagc cgccgggcgc aaaatagcgg cgcaggcagt cggagagaaa
     3121 ggccacctcc cgcgcggcgc ggcgcttgcc gtaaaaatgg gcggcgagaa acaccaccag
     3181 cgccaccacc agcgcggcca gcaaaaaggc gacgatcaaa aagcttttga gggcgggcca
     3241 gtagggctcg cggatcaggg cgccggagac cgggtcctgg cgcgtccaga taaaggtgtt
     3301 gtagaacagg tcggcgacaa agccgttcag cgcctcgtcc accaggtaca ccagcccgcc
     3361 ggtgaccagc aagaccccgg cgaggacggc cagggcgcgg gccagcgagg ggaaacggtc
     3421 tgaaaattta cgcttcaatt ttgtacccca ccccccagac tgtgcggatg tagcgcggtt
     3481 tttccgcccc ctcgcccagc ttttcacgca ggtggcggat gtgcaccatc acggtgttgt
     3541 tctcgttgcg gaaatatttt tctccccagg cctggcggaa cagctcctcc gagctgacca
     3601 cccggccgcg gttttcggcc aggatccaga ggatggaaaa ctcggtgggg gtcaggggca
     3661 cgatgcggcc gtccagcgtg cactcatggg tggcgcggtc cagcgtcagg ccgcgaaagg
     3721 cgagggtctc ctcggcgcgg gcggcctcgg cggggttgta ttttgtgtag cgccgcagct
     3781 gcgccttgac gcgggcgatc agctccagcg gacgaaaggg cttggtgaca tagtcgtccg
     3841 ccccaagggc caggccgttg atcttgtcca gctccccctc ccgcgcggtg agcagcagca
     3901 ccgggtagtt gtactgctcg cggatcttct ggcagaggga aaaaccgctg gtgtccggca
     3961 gcatcacgtc caaaaccgcc aggtcgaagg gccc
//
LOCUS       DBMDYA010000193         3978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5320_length_3978_cov_1.680165, whole genome shotgun sequence.
ACCESSION   DBMDYA010000193 DBMDYA010000000
VERSION     DBMDYA010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_3978_cov_1.680165"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="ACZPFK_05945"
     CDS             complement(<1..526)
                     /locus_tag="ACZPFK_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA ATPase domain-containing protein"
                     /protein_id="HIR1405886.1"
                     /translation="MDSFDEVFAAVKGYCRERLVDATYNLFIDGLEPVSFENGTVTIS
                     VRSDFIKGMVQERYADLLNEAFTAILGFGVDMNFQVPEPARPEPQTVVPTGSYAFTFE
                     NFIVGSTNKFAHAAAQAVAANPSGAYNPLFIYGDSGLGKTHLLNAIRLEIAKNHPDFN
                     IVYVDGEAFTNEIIG"
     gene            1149..1283
                     /gene="rpmH"
                     /locus_tag="ACZPFK_05950"
     CDS             1149..1283
                     /gene="rpmH"
                     /locus_tag="ACZPFK_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1405887.1"
                     /translation="MKRTFQPKKRQRTREHGFLSRMKSKSGRKVIAARRSKGRAKLAV
                     "
     gene            1422..1757
                     /gene="rnpA"
                     /locus_tag="ACZPFK_05955"
     CDS             1422..1757
                     /gene="rnpA"
                     /locus_tag="ACZPFK_05955"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1405888.1"
                     /translation="MRYESIVRNTEFSRAYRRGKSYVSPFLVTYVLRRKRGGVRLGIT
                     TSKKIGGAVQRVRARRVIREAYYAMGLDEGQSVDLVFVARSATTRVKMQKVEKAMRAH
                     LTAAGLLPK"
     gene            1754..2014
                     /gene="yidD"
                     /locus_tag="ACZPFK_05960"
     CDS             1754..2014
                     /gene="yidD"
                     /locus_tag="ACZPFK_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1405889.1"
                     /translation="MIRRALLALLRGYKKYLSPALGNNCRFLPTCSEYAMQAIEVHGV
                     LKGGLLSLWRVLRCNPLGRYGYDPVPEKGRWRSDERNLKKPR"
     gene            2076..3251
                     /locus_tag="ACZPFK_05965"
     CDS             2076..3251
                     /locus_tag="ACZPFK_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1405890.1"
                     /translation="MQFLGFLGGPLGYVMEFIYKFIVSDYGLSLVLFTIVLRVLMFPL
                     RIKQQKSTAKMSAYQPMILEIQKKYAKDKNKQQEELMKLQEEYGYSPTAGCLPMVLNF
                     VVIFGIIEVVYRPLTYILHLPAEVITAAADAGSIAAGYAQQSGIISAVVTGNSAVMGA
                     LGDSLSAVQGFNVFWGNLNLAAMPTISLAGWMTLIFPILSVVTMVASQIIIQKTSGQE
                     MQGSMKWMPWIMSAMFIFVGFTVPVGFSLYYTVSNVLMVVESLIAKKIYDPEKMKAQL
                     AAEIEEKRKAKKAKKKVTVKTDDGAEIKKEVTESELAAIRLQRAREIDAERYADERTD
                     PLTEEERAALEAEQNSKKKKKGRKDEAEKVSADSEAETERLLAEEKAESEKLHEDEK"
     gene            3275..>3978
                     /locus_tag="ACZPFK_05970"
     CDS             3275..>3978
                     /locus_tag="ACZPFK_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HIR1405891.1"
                     /translation="MSELIVTAKTVDQAIDKACAQLGLSRDEVSVEVLEMPTRRLFSS
                     TPAKVKVTYGSEEEQPAPVEPEREPPAEAPAPQEQEKAQPAPAAAEPAAPAAPAAPAE
                     QAAQAQPAAEPATGEKVEAAVAYFKAVAAQMGVEGFTVEPVRVNGAVILRVDGPNVGA
                     LIGRRGETMESLSYLVGLAGNRAGGDYEKFGLDVAGYRGKREKDLDALARRVGAKVQK
                     TGRSHMFEPMNPYERR"
BASE COUNT          889 a         1167 c         1251 g          671 t
ORIGIN      
        1 ccccgatgat ctcgttggta aaagcctcgc cgtccacgta gacgatgttg aaatccgggt
       61 ggttcttggc gatctcaagg cggatggcgt tgagcagatg ggtcttgccc aggccggaat
      121 cgccgtagat aaacagcggg ttataggcgc ccgaggggtt tgcggcaacc gcctgcgccg
      181 ccgcgtgggc gaatttgttg gtgctgccga cgataaaatt ttcaaaggtg aaagcatagc
      241 tgccggtggg caccacggtc tggggctcgg gacgggccgg ctccggcacc tgaaagttca
      301 tgtccacccc aaagcccagg atggcggtaa aggcctcgtt gagcaggtcg gcataccgct
      361 cctgcaccat gcccttgata aaatcgctgc gcaccgagat ggtgaccgtc ccgttctcga
      421 acgaaaccgg ctccagcccg tcgatgaaga gattgtaggt ggcgtccacc agccgttctc
      481 tgcaatagcc cttgacggct gcgaagacct cgtcaaacga atccataact gtacgacaca
      541 ctcccaatga atcagattgt ttcccgggga aaggaggcaa tgccaacggc cgttttgcgg
      601 ctgcgccgga aaagcggacc ggatcaaaca aaaaaaaaca ggcctttaag taagcccgcg
      661 ccgggagata ctacagcagc ggcgcgcccc cgcaaaaact taaaaaccgg ttttcagccg
      721 ccctccgcca aaggatgggg gccggcccgc ggccgcaaag cgttcagtga tttttcacca
      781 tagcggccgt tatgtttgta gcaattatac cataaaaaat gcgtccgtac aacacccggc
      841 aggctattta ttaatagtaa tatataagaa aattcattat ataggggtac ctgaaaaaag
      901 agcctcttta ttcattggca aaaaatgggc gctttaagcc gcgggcgcta aaaaagccgc
      961 gccgccgcag ggggaaacct tggaaaaaac tattgacagg gccgagagct ttacgctata
     1021 cttttaagtt gcaaggctgt gcgttttcag cggtgagctt gacacgcggc ctcaacgcga
     1081 tgtaacgccc tttgttcggc gtacaaataa cgcccgccag ggcagacctg aaaatggagg
     1141 aaataaaaat gaagagaacc tttcagccca aaaagcgtca gcgcacccgt gagcacggct
     1201 ttctgagccg catgaaatcc aaaagcggcc gcaaggtcat cgccgcgcgc cgcagcaagg
     1261 gccgcgccaa gctcgccgtc tgaccgcata agggggtcgg cggccgcccg cctcatccca
     1321 ctttgtaaaa gggccgccgc agcaggtggc cttttcctct tacatggggg gatgcgcggg
     1381 gcgcgccgca gccgcaaaaa ggggcagagg aggggagggc cgtgcgctat gaatccattg
     1441 tccgcaacac ggaattttcc cgcgcctacc gcaggggaaa gtcttatgtg agcccatttc
     1501 tcgtcaccta tgtgctgcgc cgcaagcgcg gcggggtgcg gctgggcatc accaccagca
     1561 aaaagatcgg cggcgcggtg cagcgggtgc gcgcgcgccg cgtgatccgc gaggcctatt
     1621 atgccatggg gctggacgag gggcagagcg tggatttggt ctttgtcgcc cgcagcgcca
     1681 ccacccgcgt caagatgcaa aaggtggaaa aggccatgcg cgcccatctg acggcggcgg
     1741 gcctgctgcc gaagtgatcc gccgggcgct gctggcgctg ctgcgcggct acaaaaaata
     1801 cctctccccg gccctgggca acaactgccg ctttctcccc acctgcagcg aatacgccat
     1861 gcaggccatc gaggtgcacg gcgtgctcaa gggcgggctg ctcagcttgt ggcgcgtcct
     1921 gcgctgtaat ccgctgggcc gctacggcta cgacccggtg cctgaaaagg ggcgctggcg
     1981 cagcgatgaa cgcaacctca aaaaaccccg ctgagaaaaa attttttcag gggaaaaaat
     2041 atcaggccgc cgcaggcggc agttaggatg tcactatgca gtttttagga tttttgggcg
     2101 ggcctctcgg gtacgtcatg gagtttatct acaagttcat cgtcagcgac tacggcctgt
     2161 cgctggtgtt gttcaccatc gtgctgcggg tgctgatgtt ccccctgcgc atcaagcagc
     2221 agaaatccac cgccaagatg tcggcctatc agccgatgat ccttgagatc cagaaaaaat
     2281 atgccaagga caagaacaag cagcaggagg agctgatgaa gcttcaggag gaatacggct
     2341 attcccccac ggccggctgc ctgccgatgg tgctgaactt tgtggtcatc ttcggcatca
     2401 tcgaggttgt ctaccgtccg ctgacctata tcctgcacct gccggcggag gtcatcaccg
     2461 ctgccgcaga cgccggctcc atcgccgcgg gctacgccca gcagagcggc atcatcagcg
     2521 ccgtcgtcac cggcaactcg gccgtcatgg gcgcgctggg tgattcgctg tccgcagtgc
     2581 agggcttcaa cgtgttctgg ggcaacctga atctggccgc catgcccacc atcagccttg
     2641 ccggctggat gaccctgatc ttccccatcc tgtcggtggt gacgatggtc gccagccaga
     2701 tcatcatcca gaagaccagc ggccaggaga tgcagggcag catgaagtgg atgccctgga
     2761 tcatgagcgc catgttcatc ttcgtcggct ttacggtgcc ggtcggcttc agcctttatt
     2821 acaccgtctc caacgtgctg atggtggtgg agagcctgat cgccaagaag atttatgacc
     2881 ccgaaaagat gaaagcgcag ctcgccgccg agatcgagga gaagcgaaag gccaaaaagg
     2941 ccaaaaagaa ggtcaccgtc aagactgacg acggcgctga gatcaaaaaa gaggtcaccg
     3001 agtctgagct ggccgccatc cgcctgcagc gcgcgcggga gatcgacgcc gagcggtatg
     3061 ccgacgagcg caccgatccc ctgaccgagg aggagcgcgc cgccctggaa gcggagcaga
     3121 actccaaaaa gaaaaagaaa ggccgcaagg acgaagcgga gaaagtctcg gcggattccg
     3181 aggcggagac cgagcgcctg ctggccgagg aaaaagcgga gagcgaaaag ctgcacgagg
     3241 acgagaaata aacgcgggag aggagtaaac ggcaatgagt gaattgatcg tgactgcaaa
     3301 aaccgtggat caggcgatcg acaaggcctg tgcacagctg ggcctgagcc gggacgaggt
     3361 gagcgtcgag gtgctcgaga tgcccacccg gcgtctgttt tcctccaccc ctgccaaggt
     3421 gaaagtcacc tacggcagcg aggaggagca gccggccccg gtggagccgg agcgggagcc
     3481 cccagcggaa gcccccgcgc cgcaggagca ggaaaaggcg cagcccgccc ccgcggcggc
     3541 agagcccgcc gcccctgcag cccctgcagc ccctgcggag caggccgcgc aggcacagcc
     3601 cgcggcggag cccgccaccg gcgaaaaggt ggaggccgcc gtcgcctatt ttaaggccgt
     3661 cgccgcccaa atgggcgtcg aggggttcac cgtggagccg gtgcgggtga acggcgcggt
     3721 catcctgcgg gtggacggcc ccaacgtggg ggccctgatc ggccgccgcg gcgagaccat
     3781 ggagtcgctg agctatctgg tgggcctggc cggcaatcgc gccggtgggg attatgagaa
     3841 gttcggcctg gatgtggccg gataccgcgg caagcgggaa aaggatctgg acgcgctggc
     3901 gcgccgcgtg ggcgccaagg tgcagaagac cggccgcagc cacatgtttg agcccatgaa
     3961 cccctatgag cgccgcat
//
LOCUS       DBMDYA010000194         3976 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5322_length_3976_cov_1.606710, whole genome shotgun sequence.
ACCESSION   DBMDYA010000194 DBMDYA010000000
VERSION     DBMDYA010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5322_length_3976_cov_1.606710"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="ACZPFK_05975"
     CDS             <1..456
                     /locus_tag="ACZPFK_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405892.1"
                     /translation="NGATRVLVCVDDLRDEEPGGRWYNPYQPQAVRFESVLQLLNGME
                     ELYDDLSFPQPAFCRRSWRQGKETRDRYRSRKKEVKRYMSDEVFQEKRGEKATFVVQV
                     QFRQNATWQGTVTWTEKNETRHFRSTLELIRLMDESLSMGQDEPAETAT"
     gene            544..>3976
                     /locus_tag="ACZPFK_05980"
     CDS             544..>3976
                     /locus_tag="ACZPFK_05980"
                     /inference="COORDINATES: protein motif:HMM:NF049299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7601 domain-containing protein"
                     /protein_id="HIR1405893.1"
                     /translation="MKKKFLSALLAIAILATSLPLNLFSAVTAGGDNEAAAPGVYNGE
                     SVTKDGVTLSKEAEQVGLDEYEITLKLTGGGTALERQPMELVFALDSSSSMVEKVKVT
                     KRGECGETIRRYENTTSSIKSGDSYWEWGSLKRPFTETETMTVTYRCDDGHVTSTYTA
                     TRTRRGTQTYWAFGGWQVSGNPSWSDWKWSSPDKNIEGQKCKTEVTGTGTESVQRIKR
                     ARSAMKELLRKLYDNKIDARIRMIHFAGNSEELTSDWIDLMKEQDGALVSDQAALEKL
                     LSMIDNGWRPLTGGDNDSKRAIYGKTGGTNIQQALSHADSLFSAGNEVPQNLLLVSDG
                     GPTTYNKGGTIGYWCGDHSSYCPDAAQAALNKATAVKNAHRGNPDFSLYAVAVDITGD
                     SAKKFFPQLASPGCSLEASSDELSGEFMKFVTKITQLVNDGVVTDPMGEDVDLKDVSG
                     VTVSPSVLYDDDGKALPADQQPSFKFENGVLEWYTGAYLKKGEEYTLTYRVKLNNIES
                     PGQYVPLNGATSLTYEDKDGKSHDVGFNVPQVAYATGTLLVTASGLPEDAVLHEQTDG
                     PKLVGTAEFSNEFDTVRHAEQIVDNLPAEYELQQVKVTRGEDANTQTLTPDEFEAWLK
                     ENSSRKGKGILDVVSGETKIEYVYGVKRGDFSFAKTLAAGLPQDTDSEFTFKVELENG
                     KYDLDGETISYTVAGTEHTETLKRSFFGKYSFEVKLKAGQTVQVTKVPVGTSYTVSET
                     GTGDYVELADTMTGTVRGGNGASGTFTNNKEFELTVQHVYTAFDGTKTTVTSKDKVPT
                     LFGAAILETKADKDGYVFKDVSMLESYDEIIDPATGTLTATMPARDATILFEYIPRSD
                     LSYTVEYRENSETGAELHAPKTETGKTFGQTYTEEAVEIQGYKVDSETKSVTMTTGTN
                     KIVFIYTRDDDQTQPTEYTVKHTIEGVEQTADTQTYEGKAWINEEHPTIVVEAGSLAH
                     KTYTGYKFEKMTPKTKEGDSVASGTTIILNYVRDDNQTQPTKYTVQHKVDGEIRDTKT
                     YTGTAWINETNPTIEIETGSLAKKTYTGYTFSSIDTTAQEGDKIASGTVITLTYVKDD
                     GQTQPTKYTVRHVVDGVEQTADTKTYTGTAWINEKNPTIKIEAGSLAKKTYTGYTF"
BASE COUNT          972 a         1207 c         1233 g          564 t
ORIGIN      
        1 aacggcgcga cccgtgtgct ggtctgtgtc gacgatttgc gtgatgagga gccgggcggc
       61 aggtggtaca atccctacca gccccaggcg gtgcggtttg aaagcgtgct gcagctgctc
      121 aacggcatgg aggagctcta cgacgacctg tctttcccgc agcccgcgtt ctgccgccgc
      181 agctggcggc agggcaaaga gacccgggac agataccgaa gccggaaaaa agaggtgaag
      241 agatacatga gcgacgaggt ctttcaagag aaacgaggcg aaaaggccac ctttgtggtg
      301 caggtacagt tccggcagaa cgccacctgg cagggcaccg tgacctggac cgaaaaaaac
      361 gagacgcggc atttccgcag cacgctggag ctcatccgcc tcatggacga gagcctcagt
      421 atgggacagg acgaacccgc cgaaaccgcg acgtagacag ccaggcactg gcatccccgg
      481 ccggacggcc ggttcaaata gattaacacc ctatacaagt taggtaaaga aagggttgaa
      541 gaaatgaaga aaaagttttt gagcgccctg ctggcgatcg ccatcctggc gaccagtctg
      601 ccgctgaacc tgttcagcgc ggtcacagcg ggcggcgaca acgaggcggc cgcccccggc
      661 gtctataacg gcgagagcgt caccaaggac ggcgtcacgc tcagcaagga ggccgagcag
      721 gtcggcctgg acgagtatga gatcaccctc aagctcaccg gcggcggcac cgcgctggag
      781 cgccagccca tggagctggt cttcgcgctg gactcctcct ccagtatggt ggagaaggtc
      841 aaggtcacca agcgcgggga gtgcggcgag acgatccgcc ggtatgagaa cacgacgtct
      901 tcgataaaat ccggcgacag ctactgggag tggggaagtc tcaagcggcc tttcaccgag
      961 accgaaacca tgacggtcac ctaccgctgc gacgacggcc acgtgacgag tacctatacc
     1021 gccacccgca cccgccgcgg cacccagacc tattgggctt ttgggggctg gcaggtcagc
     1081 ggcaacccca gctggagcga ctggaaatgg tccagccccg acaagaacat cgaggggcag
     1141 aagtgcaaaa ctgaggtcac gggcacgggc acggagagcg tccagcgcat caagcgcgcc
     1201 cgctcggcga tgaaagagct tctgcgcaag ctgtacgaca acaagatcga cgcgcgcatc
     1261 cgcatgatcc acttcgcggg caactcggaa gaattgacct ccgactggat cgatctgatg
     1321 aaagagcagg acggcgcgct ggtgtcggat caggcggcgc tggagaagct gctgagcatg
     1381 atcgacaacg gctggcggcc cctgaccggc ggcgacaacg acagcaaacg cgctatttac
     1441 ggtaaaaccg gcggcaccaa catccaacag gcgctgagcc atgccgacag cctgttcagc
     1501 gcgggcaacg aggtgcccca gaacctgctg ctggtcagcg acggcggccc caccacctat
     1561 aacaaaggcg gcaccatcgg ttactggtgt ggcgatcact ccagttattg ccccgacgct
     1621 gcgcaggcgg cgctgaataa ggcgacagcc gtcaaaaacg cccaccgcgg caacccggat
     1681 ttttcgctgt acgcggtggc ggtggacatc acgggagaca gcgcgaagaa attcttcccg
     1741 cagcttgcca gccccggctg ctcgctggag gccagcagcg acgagctcag cggcgagttt
     1801 atgaagttcg tcaccaagat cacccagctg gtcaacgacg gcgtggtcac cgacccgatg
     1861 ggcgaggacg tggatctcaa ggacgtgagc ggcgtcaccg tcagcccgtc cgtactctat
     1921 gacgacgacg gcaaagcgct gccggcggac cagcagccct cgttcaaatt cgagaacggt
     1981 gtgctggaat ggtataccgg cgcttacctg aaaaagggtg aggaatacac cctcacctat
     2041 cgggtgaagc tgaacaacat cgagagcccc ggccaatatg tgcccctgaa cggcgccacc
     2101 agcctgacct atgaggacaa ggacggcaaa agccacgacg tgggcttcaa tgtgccgcag
     2161 gtggcctatg ccaccggcac cctgctggtg accgccagcg gcctgccgga ggatgcggtg
     2221 ctgcacgagc agaccgacgg ccccaagctg gtgggcacgg cggagttcag caatgagttt
     2281 gacacggtgc ggcacgctga acagatcgtc gacaacctgc ccgccgagta cgagctgcag
     2341 caggtgaaag tgacccgcgg cgaggacgcg aacacccaga ccctcacccc cgacgagttt
     2401 gaggcatggc tgaaagaaaa ttcttcccgc aagggcaagg gcatcctgga cgtggtgtcc
     2461 ggcgagacca agatcgaata tgtctacggc gtcaagcgcg gcgatttcag ctttgccaag
     2521 acgctggccg ccggcctgcc ccaggacact gacagcgagt tcacgttcaa ggtggagctt
     2581 gagaacggca agtatgacct ggacggcgag accatcagct ataccgtcgc cggcacagag
     2641 cataccgaga cgctgaagcg gtctttcttt ggaaagtatt cctttgaggt gaagctcaag
     2701 gcgggccaga ccgtccaggt cacgaaagtg cccgtgggca ccagctacac cgtctccgag
     2761 accgggacgg gcgactacgt cgagctggcc gacaccatga ccggcacggt caggggcggc
     2821 aacggcgcca gcgggacgtt caccaacaac aaagagtttg agctgaccgt ccagcacgtc
     2881 tataccgctt ttgacggcac aaagacgacg gtcacctcca aggataaggt gccgaccctg
     2941 ttcggcgccg cgatcctcga gacaaaggcc gacaaggacg gatatgtgtt caaggacgtc
     3001 agcatgctgg agtcctacga cgaaataatc gaccctgcga ccggcaccct caccgcgacg
     3061 atgcccgccc gggatgcgac gattctcttc gagtatatcc cgcgcagcga tctgagctac
     3121 acggtggagt accgcgagaa cagcgagacc ggcgccgagc tgcatgcccc caagacggag
     3181 accggcaaaa ctttcggcca gacctatacc gaagaagccg tcgagatcca gggctacaag
     3241 gtggacagcg agaccaagtc ggtcacgatg acgaccggta ccaacaagat cgtcttcatc
     3301 tacacgaggg acgacgacca gacccagccc accgagtaca cggtgaagca cacgatcgag
     3361 ggtgtggagc agaccgcgga cacccagacc tacgagggca aggcgtggat caacgaggag
     3421 cacccgacca tcgtggtcga ggcgggttcg ttggcccata agacctacac gggctacaag
     3481 ttcgagaaga tgaccccgaa gaccaaagag ggcgattccg tcgccagcgg cacgaccatc
     3541 atcctgaact atgtgcggga cgacaaccag acccagccga cgaagtatac ggtgcagcac
     3601 aaggtggacg gcgagatccg cgataccaag acctacaccg gcacggcgtg gatcaatgaa
     3661 acgaacccga ccatcgaaat cgagacgggc tcgctggcca agaagaccta cacgggctac
     3721 acattcagca gcatcgacac gacggcccag gagggcgaca agatcgccag cggcacggtc
     3781 atcaccctga cctatgtgaa agacgatggc cagacccagc cgacaaagta cacggtgcgg
     3841 cacgtggtgg acggcgtgga gcaaactgcg gacaccaaga cctacaccgg cacggcgtgg
     3901 atcaacgaga agaacccgac catcaagatc gaagcgggct cgctggccaa gaagacctac
     3961 acgggctaca cattca
//
LOCUS       DBMDYA010000195         3962 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5334_length_3962_cov_1.694898, whole genome shotgun sequence.
ACCESSION   DBMDYA010000195 DBMDYA010000000
VERSION     DBMDYA010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5334_length_3962_cov_1.694898"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="ACZPFK_05985"
     CDS             <1..749
                     /locus_tag="ACZPFK_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1405894.1"
                     /translation="TGGINLADEYINRVVKHGHWKDTVVRLQGDAVYSLTMMFLSLWD
                     YCEGAVEDYDAYRPQVHAPAPAVGDCWVQPFGDSPFDDEPAGENVYLNLLGAATDRVW
                     INTPYLIIGNEMVSALAGAAKRGVDVRIVTPHVADKKFVHAMTRSYYLELIEAGVKIY
                     EYTPGFIHAKSFVVDGKTAVIGTINLDYRSLYLHFECGVWMYRTPCIRDMERDYLQTL
                     EKSRPVTLEECRGLPLRTRLRARCCASSPR"
     gene            complement(944..2155)
                     /locus_tag="ACZPFK_05990"
     CDS             complement(944..2155)
                     /locus_tag="ACZPFK_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405895.1"
                     /translation="MNATSVSQKKRLVIATAFTFVFMAGYALSAGMLGTLMPRIIEYY
                     GLTLSAASSINIAKEVGNTASMIFALLVVDRLDKNRLLAVMGLCFGGALLLFGTAPAF
                     ALLLAVQLVIGFSGGLLDNLCATYVSDLYGERRARFVSILHTLFALGNMAGPKFAAWC
                     YSLGGWTLSFLTSGIALAAAAVLFFVLTRLVGPPDAAVKKAPGQVRQNIPYGKILKGK
                     NLWYLSIASMFFSGLTYLTVWLPTYLDWRDPSVYTTDFCATVMTAYSLGMLISRTGLA
                     ALSEKISTATYVKWSSLLAALLFAVLLVVQQPLVWTIGIFLFGVISGANYTARFVLSC
                     QEYPEYSSTASAFTGAFAALGNILYSAGIGALADAGYYTQALFMIVGALGLGFVIFTF
                     LYRSPERRRQS"
     gene            complement(2934..3962)
                     /locus_tag="ACZPFK_05995"
                     /pseudo
     CDS             complement(2934..3962)
                     /locus_tag="ACZPFK_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519861.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
BASE COUNT          779 a         1250 c         1191 g          742 t
ORIGIN      
        1 ttaccggcgg catcaacctg gcggacgaat acatcaaccg cgtcgtcaag cacggccact
       61 ggaaagacac cgtcgtgcgc ctgcaggggg acgcggtcta cagcctgacc atgatgttcc
      121 tctcgctgtg ggattactgc gagggcgccg tggaggacta cgacgcctac cgcccccagg
      181 tccacgcccc cgcccccgcc gtgggggatt gttgggtgca gccctttggc gactcgccgt
      241 ttgacgacga gcccgccggc gaaaacgtct atctcaatct gttgggcgcg gcgaccgacc
      301 gggtctggat caatacgccc tatctcatca tcggcaacga gatggtgtcg gccctggcgg
      361 gcgcggctaa gcgcggggtg gatgtgcgca tcgtcacccc gcacgtggcg gacaaaaaat
      421 tcgtccatgc catgacccgt tcttactatc tggagctgat cgaggccggg gtgaaaatct
      481 acgaatacac ccccgggttc atccacgcaa aaagctttgt agtagacggc aagacagccg
      541 tgatcggcac catcaacctg gattaccgca gtctctatct ccactttgaa tgcggcgtgt
      601 ggatgtatcg caccccgtgt atccgggaca tggagcggga ttatctgcag accctggaga
      661 agagccggcc ggtcacgctg gaggagtgcc gcggcctgcc cctgcgcacc cgtctgcgcg
      721 cgcggtgctg cgcatcttcg ccccgctgat gtagggcgcc agggcgtgaa agcgcttcat
      781 gccgggaccc gctttccgcc gcaaacagcc ggataccgag aggcctcgtc tcttttgggt
      841 gactccagct tcaacagcaa aattcaaaaa gggcgtccag gcacagagcc ctggacgccc
      901 ttttggttct ccgcgcgggg cgcgcatttt tccaattttt cgctcagctc tgtctgcggc
      961 gctccggcga ccggtacagg aaagtgaaga tcacaaagcc cagccccagc gcgcccacga
     1021 tcataaagag ggcctgggtg taatagcccg cgtcggccag cgcgccgata ccggcgctgt
     1081 acaaaatatt gcccagcgcg gcaaacgccc cggtaaaggc ggaggcggtg gagctgtact
     1141 cggggtactc ctggcaggag agcacgaagc gggcggtgta atttgcgccg ctgatgaccc
     1201 cgaacagaaa gatgccgatc gtccagacaa ggggctgctg caccaccaga agcaccgcaa
     1261 aaagcagcgc agccaacagc gacgaccact ttacgtaggt ggcggtgctg attttttcag
     1321 acagcgccgc aaggccggtg cggctgatca gcatgcccag cgagtaggcg gtcatcacgg
     1381 tggcgcaaaa atccgtggtg tagaccgacg ggtcccgcca atcgagatag gtgggcagcc
     1441 agacggtgag ataggtcagc ccggagaaaa acatgctggc gatggagaga taccagagat
     1501 ttttgccttt caggatcttg ccgtagggga tgttctgccg cacctggccg ggcgcttttt
     1561 ttacggcggc gtccgggggg ccgaccagcc gggtgaggac aaaaaagagc accgcggccg
     1621 cggccagcgc gatgccgctg gtcaaaaagc tgagggtcca gccgcccagg gaatagcacc
     1681 aggcggcaaa cttcggcccg gccatattgc ccagcgcgaa cagggtgtgc aggatggaga
     1741 caaagcgtgc ccgccgctcg ccgtacaggt cggagacata ggtggcgcag aggttgtcca
     1801 gcaggccgcc ggaaaagccg atgaccagct gcaccgccaa cagcagcgca aaggcgggcg
     1861 cggtgccgaa cagcagcagc gcgccgccaa agcaaagccc catcaccgcc agcagccggt
     1921 ttttgtccag ccggtccacc accagcagcg caaagatcat agaggcggta ttgcccacct
     1981 ccttggcgat gttgatggag gaggccgcac tcagggtgag gccgtaatac tcgatgatgc
     2041 ggggcatcag ggtgcccagc atgcccgccg aaagggcgta gcccgccata aagacaaagg
     2101 tgaatgccgt ggcgatcacc agccgctttt tctggctcac ggaagttgcg ttcaactatt
     2161 gttcctcctc ttttacagtt caaggtcctc tgcgcttcgc cgcgcttttc agcctgcatc
     2221 ctgcccggca ggcgcagacc acaaaggcgc aagggtgggg gcgatgggac cgcttttgac
     2281 ctggggtgcg gtcccactcc cccgccctca aatccgcccc ggccagcggg cggacggggc
     2341 ggcaaaaaac aattttgggg gacggcccgt ttttcgccgc tgacgcgaca gcagaaacgg
     2401 atggccgaaa aaacaactta ttttaatggc cccggccaga ggactggccg gatggccgaa
     2461 aaaacagcct gcggctgttt ttcattgctc cgcaacaaaa aacaacttat cttaatggcc
     2521 ccggccagag gactggccgg atggccgaaa aaacagctgc ggctgttttt tattatacct
     2581 ccctaaaaaa gccgctgtat atgagatttc tcacatgttt tccaggaaaa tgttccatac
     2641 ggcccccgca tacagaaaat aacccttttc cctttcggcc tctggcttct tctcttataa
     2701 agcgcagctc tgcgcgagct tcttttgaaa agggcgcaag gctttccgct tttcgcggaa
     2761 gctcctcccc ctcccccaaa agcgaaaagc gccggggcgt tttctccgcg tcccggcgct
     2821 cttccatcct tttctcgttc tctctgtgct ccaggcggca tcaaaggcgc cgcggcgctt
     2881 tttctctggc atggcgtcct tttttggcta cgcgccaaac ggtttcggca gcctcagacg
     2941 ctcagctcgg cccgctcgcc caacagcgcg cggttggccg ccagcaccgg gcgcaccacg
     3001 ccgtccaaaa attcttccac ctgcttcggc gcacggccga taaaatcctc cggccgcagc
     3061 agctcggcca cctcggcctc gctgagctga aaagccgggt cggccagaac ccgttcgatc
     3121 aggtcgttgc tgccgccctc ctccttgacc accttgcctg cggcgatgga gtgttggcgc
     3181 agccgctcat gcagctcttg ccggtcgccg ccctttttca ccgcctgcat catgatattc
     3241 tccgtggcca taaagggcag ctcgttcagc acccgcgccc gcaccacctt ggggtagacc
     3301 accagcccgt cgcagacgtt gagcaggatg ttcaaaatgg cgtcggccgc caaaaagccc
     3361 tcggccaccg cgatgcgctt gttggccgag tcgtccaggg tgcgctcgaa ccactgggtg
     3421 cccgcggtaa agcggggttc agcacatcca ccatcagata gcgcgccagc gcacagatgc
     3481 gctcggagcg catgggattg cgcttgtagg gcatggccga ggagccgatc tggtgggcct
     3541 caaagggctc ttccatctcc ttgaagttct gcaggatgcg cagatcgttg gcaaatttca
     3601 tcgcgctttg ggcgatgccg gccagcgcgt tgagcaccat ggcgtccacc ttgcggctgt
     3661 aggtctggcc ggagaccggc accacctttt caaagcccat ctcggcggcg atgtcgtcct
     3721 caatggcctt gatcttggcg tcgtccccgt ccagcagctc cacaaagctt gcctgggtgc
     3781 cggtggtgcc cttgctgccc agcagcgcca gcgagccgat gcggtggtcc acctcctcca
     3841 ggtccatcat cagctcgttg agccagaggg tggcccgttt gcccaccgtg gtgagctggg
     3901 cgggctgcag gtgggtgtag gccagggcag gcatctcttt gtacttttcg gcaaaatcag
     3961 cc
//
LOCUS       DBMDYA010000196         3954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5347_length_3954_cov_1.825071, whole genome shotgun sequence.
ACCESSION   DBMDYA010000196 DBMDYA010000000
VERSION     DBMDYA010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_3954_cov_1.825071"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="ACZPFK_06000"
     CDS             <1..175
                     /locus_tag="ACZPFK_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WCX domain-containing protein"
                     /protein_id="HIR1405896.1"
                     /translation="FLPDGEEHYIVSFPFIENEYYYDILFSFGDKCECLEPWAVRAEM
                     GRRLRGMAALYES"
     gene            332..1408
                     /locus_tag="ACZPFK_06005"
     CDS             332..1408
                     /locus_tag="ACZPFK_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1405897.1"
                     /translation="MLYVRTEEEFKSYPWCRRILRGLGDEARKKRVPLQEIGDTAHIP
                     RQDQEAAVLLIGASEAWIGREALRANAHGAHPIALTNRQPAGRDGALSAVMMDIPDSM
                     RLAVEYLRGLGCERLALYGVNPNSTSDPWRAQVFERLTGGRGGVFVNEGSLQQTFERC
                     RSRLESCDGVICASDYAALSLVRRLGQVGGGRPGSPYVLGYGNLFLSRLSSPSITSIS
                     DDYEHFGRAALSIYHLVAREKTVCAVNILLHSRLHIRETTGNRPCAGGRRGQEAAPQR
                     PRNLFYEDGEISRMAKLETLLGQCDETDFAVLQCLMRGDTYAATAEKCFVSETAAKYR
                     VKKMEGMCGVDSREELAAFLREFF"
     gene            complement(1567..>3954)
                     /locus_tag="ACZPFK_06010"
     CDS             complement(1567..>3954)
                     /locus_tag="ACZPFK_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1405898.1"
                     /translation="KKMWTWGHGEFGEMWCSNLTDEDGPYIELMTGVYTDNQPDFTWL
                     APYETREFEQVWYPIRGIGDVKNATVDAAMNLEQRGGEFFFGFCVTGVFPGCRITVRD
                     GGELLYEERADLDPTAPWLKTLPLDGHKFEDLTVCLLSAQGKELVSYTPYRRGQKKPI
                     EVRQPVRRPAEIATVEELYINGYHLEQYKQHNYDPRDYYREGLRRDPGDIRCNTGLGR
                     LALKDGRFEQCVAHCDLAIQRLTSRNQHPTDTEALYLKGVALCYLGRHEEAYDTLFCA
                     GWNYAHRSAAYYSLAAIDCRFGRWSPALEKLDVSLGLNAGHARAANLKAAVLRRLGRD
                     GEALALARATAAADPLDLGARFELHFLTGEGLEEIREIFGGKAENLIDVVCDKLEAGL
                     AEEALRVLDTADGGYPLLRYYRAWCLHLLGRDGGAELAAAAALETGTCFPSRLEDIAV
                     LEYAAGANAADANACYYLGCLFYDRFRYGEAVSRWEEALRRDPGHAKALRCLSLAYFD
                     KCGDAASARACMERALDLLPDPRLLFEYQQLLKNSNVPPEERLAVYDRYPRLLEQRDD
                     CYLDRIVLLCMKGRYEEAIGLARAKHFHIYEGGEGNLTKQHAWMHLLYGNELAAAGRD
                     AEAERAYRDGAEMPRSYGEAKTFFNQEAHLYFYLGLLLEKRGDGAGAKAAFEEASVYK
                     AAVSELSLFRALALRRLLRFSEAQQVLQEMLDAGDSLIENKDLRGYYGVGSPCPMPFE
                     YDIEKQNLVNGHILRGYALLGLGRGQEADAEIAAAAALDPYNFRLYAYGQIRPTL"
BASE COUNT          634 a         1289 c         1384 g          647 t
ORIGIN      
        1 ctttttgccg gacggcgagg agcactatat cgtcagcttc ccttttatag aaaacgaata
       61 ttactacgat atccttttca gcttcgggga caaatgcgag tgcctggagc cgtgggctgt
      121 ccgcgcggag atggggcgca gattgcgcgg gatggcggcc ctgtacgaga gctgacggta
      181 caaaaaaggg acagatccgt ccggcgcggg ccgggggaac gcccgctgga gagggcctgc
      241 ggtaaggccc gccgctctgc gctttcgtcc gggcccgaaa ggccctgtta tccaccgcgc
      301 ggcgcgcccg cgcaggacca ttggtgaaaa aatgctgtat gtgagaacgg aagaggagtt
      361 caagagctat ccctggtgcc ggcgcatcct ccgggggctg ggggacgagg cgcgaaaaaa
      421 gcgggtgccc ctgcaggaga tcggcgacac ggcccacatc ccccggcagg accaggaggc
      481 cgccgtgctg ctgatcggcg catcggaggc ctggatcggc cgggaagcgc tgcgggcaaa
      541 cgcgcacggc gcgcacccca tcgccctcac caaccgccag cccgcgggca gggacggggc
      601 gctcagcgcg gtgatgatgg acatcccgga ttcgatgcgg ctggccgtgg agtatctgcg
      661 cggtctgggc tgcgagaggc tggccctgta tggggtcaac cccaattcca cctccgaccc
      721 ttggcgggcc caggtttttg aacgcctgac cgggggccgg ggcggcgtgt tcgtcaacga
      781 ggggtcgctg cagcagacgt tcgagcgctg ccgcagccgg ctcgaaagct gcgacggggt
      841 gatctgcgcc agcgactacg ccgcgctttc cctggtgcgg cggctggggc aggtgggggg
      901 cggccggccc ggcagcccct atgtgctggg gtacggcaac ctgtttttgt caaggctgtc
      961 ctccccctcc atcacctcca tctccgatga ttacgagcac ttcggccgcg ccgcgctgtc
     1021 gatctaccat ctggtggcgc gggaaaagac ggtctgtgcg gtgaacatcc tgctgcacag
     1081 ccggctgcat atccgcgaga ccaccggcaa ccggccctgt gcgggcgggc gccgggggca
     1141 ggaggcggcg ccgcagcgcc cgcggaatct gttttacgag gacggcgaga tctcgcgcat
     1201 ggcaaagctg gagacgctgc tgggccaatg cgacgagacc gatttcgccg tgctgcagtg
     1261 cctgatgcgc ggcgacacct acgccgcgac cgcggagaaa tgctttgtgt ccgagacggc
     1321 cgccaagtat cgggtcaaaa agatggaggg gatgtgcggg gtggacagcc gagaggagct
     1381 ggccgctttt ctgcgggagt ttttttgaca gtgcaaagca gcccgcaccc ggctgacagg
     1441 ccgggcgcgg gctgtttttt tgtagtggac gaaagaaaaa acgaaaaggc cgccccgggc
     1501 gccgcctgcg cgcggcggcc cgggacggcc cggagagagc gcccctgtcg ggggctgaga
     1561 ttgtgcttag agcgtgggac ggatctggcc gtaggcgtaa agccggaagt tgtaggggtc
     1621 cagcgcggcg gccgcggcga tctcggcgtc cgcctcctgc ccgcggccca ggcccagcag
     1681 cgcgtaaccc cgcaggatgt gcccgttcac cagattctgc ttttcaatgt cgtattcaaa
     1741 gggcatgggg cagggggaac cgaccccgta atagccgcgc aggtccttgt tctcgatcag
     1801 gctgtcgccg gcgtccagca tctcctgcag cacctgctgc gcctcggaga agcgcagcag
     1861 ccggcgcagc gccagcgccc ggaacaggga gagctccgag acggccgcct tgtagaccga
     1921 ggcctcctca aaggcggctt tcgccccggc cccatcgccc cgcttttcca gcagcaggcc
     1981 caggtagaaa tagagatggg cctcctggtt gaaaaaggtc ttggcctcgc cgtagctgcg
     2041 cggcatctcg gcgccgtccc ggtaggcccg ctccgcctcg gcgtcccggc ccgcggcggc
     2101 cagctcgttg ccgtagagca ggtgcatcca ggcgtgctgc ttggtcaggt tgccctcgcc
     2161 gccctcgtag atgtggaagt gcttggcgcg ggcaagcccg atggcctcct cgtagcgccc
     2221 tttcatgcac agcagcacga tgcggtccag ataacagtcg tcccgctgct ccagcagccg
     2281 gggatagcgg tcgtagacgg cgagccgctc ctccggcggg acattgctgt ttttcagcag
     2341 ctgctggtat tcaaacagca gccgcgggtc gggcagcagg tccagggcgc gctccatgca
     2401 ggcccgggcg gaggcggcgt ccccgcactt gtcgaagtag gcgaggctga ggcagcgcag
     2461 ggccttggcg tggccggggt cgcggcgcag cgcctcctcc cagcgggaga cggcctcccc
     2521 gtagcggaag cggtcgtaga acaggcagcc cagatagtag caggcgttgg cgtccgcggc
     2581 gttggcaccc gccgcgtatt ccagcacggc gatgtcctcc agccgggagg ggaagcaggt
     2641 gcccgtctcc agggcggccg cggccgccag ctcggcgccg ccgtcccggc ccaggagatg
     2701 caggcaccag gcgcggtagt agcgcagcag cggatagccg ccgtccgccg tgtccagcac
     2761 ccgaagcgcc tcctccgcca gacccgcctc cagcttgtcg cagaccacgt cgatcaagtt
     2821 ctccgccttg ccgccgaaga tctcgcggat ctcctccagc ccctcgccgg tgaggaagtg
     2881 cagctcaaag cgggccccca ggtccagcgg gtctgccgcc gccgtcgccc gcgccagggc
     2941 cagcgcctcg ccgtcgcgcc ccagacggcg cagcacggcg gctttcaggt tggctgcccg
     3001 ggcgtggccg gcgttcagcc ccagcgagac gtccagcttt tccagcgccg gggaccagcg
     3061 gccgaagcgg cagtcgatgg ccgccaggct gtaataggcg gcgctgcggt gggcatagtt
     3121 ccacccggcg caaaacagcg tgtcgtaggc ctcctcgtgg cggcccagat aacagagggc
     3181 gacgcctttc agatagaggg cctcggtatc ggtggggtgc tggttgcggc tggtgagccg
     3241 ctggatcgca aggtcacagt gggccacaca ctgctcgaag cggccgtctt tcagggccag
     3301 gcggcccagg ccggtgttgc agcggatgtc gcccgggtcg cggcgcagcc cctcgcggta
     3361 gtagtcgcgc gggtcatagt tgtgctgctt gtactgctcc agatggtagc cgttgatgta
     3421 cagctcctcc accgtggcga tctcggcggg gcggcgcacc ggctggcgca cctcgatggg
     3481 ctttttctgg ccgcgccggt agggcgtgta ggagaccagc tcctttccct gcgccgagag
     3541 caggcagacg gtcaaatcct caaacttgtg gccgtccagc ggcagggttt tcagccaggg
     3601 ggcggtgggg tccaggtcgg cgcgctcttc atacagcagc tcgccgccgt cccgcacggt
     3661 gatgcggcag ccggggaaga cgccggtgac gcagaagccg aaaaagaact ccccgccccg
     3721 ctgctccagg ttcatggccg cgtccaccgt ggcgtttttc acgtccccga tgccccggat
     3781 gggataccac acctgctcga actcccgcgt ctcgtagggg gccagccagg tgaagtccgg
     3841 ctggttgtcg gtgtaaacgc cggtcatcag ctcgatgtag gggccgtcct cgtcggtgag
     3901 gttcgagcac cacatctcgc cgaactcccc atggccccag gtccacattt tttt
//
LOCUS       DBMDYA010000197         3952 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5348_length_3952_cov_1.427681, whole genome shotgun sequence.
ACCESSION   DBMDYA010000197 DBMDYA010000000
VERSION     DBMDYA010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5348_length_3952_cov_1.427681"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            480..980
                     /locus_tag="ACZPFK_06015"
     CDS             480..980
                     /locus_tag="ACZPFK_06015"
                     /inference="COORDINATES: protein motif:HMM:NF047079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6933 domain-containing protein"
                     /protein_id="HIR1405899.1"
                     /translation="MQLGLTIPLQRYLRAPRPPAPPAAERLFCWDLHRILLRGEGSLL
                     AVHCASRFAVVVFGLSAVDWASLDGVVPAQICRGLLDADLPQAPVRAYLERCGPPCFT
                     RTHGRREVAFLNRAWEDVMACDFALDRALPGQPLLERAVNALPRRCAGQEGLASSRER
                     LLQLLV"
     gene            complement(1052..1846)
                     /locus_tag="ACZPFK_06020"
     CDS             complement(1052..1846)
                     /locus_tag="ACZPFK_06020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1405900.1"
                     /translation="METNPYLVDFYHHYDEDSRLTSKHGRVEFFTTMRYIEKYLKPGS
                     RILEIGAGTGRYSHALARQGYAVDAVELVEHNIEIFRKNTSAEEQVSVLQGNALDLSA
                     FSSNRYDVTLLLGPLYHLYDQESKRRAIREAIRVTKPGGVIFAAYVISDGCLLDEGFK
                     RGSVNVAEYIKNGLLDPETFAAESQPKDLFELVRKEDVDELMSAFFVKRLHYVAADGC
                     ALLIREAIDAMDDKTFDLYLNYHFATCERKDLLGVTSHALDVFQKQ"
     gene            complement(1848..2429)
                     /locus_tag="ACZPFK_06025"
     CDS             complement(1848..2429)
                     /locus_tag="ACZPFK_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1405901.1"
                     /translation="MPPKPKITKDMILNTVLKITREMGFEAVNARSVAGRLECSTRPI
                     FTCYKNMEELKKDFLDFAFEYYSRYAAEYRKAKTIKPYLFWPLSYIEFAKEETNLFKL
                     LFINDMELDMAKAEDFYKELENEEKANEFSEMIGVRPEGGKRIFLDLFLYTHGIAVLT
                     ATGKLSLGRRDSEKMLTEFLSAYVEQETEKERA"
     gene            complement(2798..3715)
                     /locus_tag="ACZPFK_06030"
     CDS             complement(2798..3715)
                     /locus_tag="ACZPFK_06030"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR1405902.1"
                     /translation="MAIPKYDEMYRAFMDCLADMQVHKSKEMKELLAAKFQISDEERH
                     ILLPSGHRPIFDNRVGWTRTYLKKAGLIDSPSRGAYQLTQTGKQVLVEDPPVLDNHYL
                     EQFDSFCQFIGSDSSVPAQSLPALSGDSPQDILDDAYQKINDALYDDLLAEIMKQTPA
                     FFEALVVKLLERMGYGGSLENAGMVVGQSGDEGIDGIIREDKLGFSLIYIQAKRWELL
                     QTIGRPEIQRFVGALAGQGASKGLFITTARFTREAREYADKQHTTKVVLVDGHMLAKL
                     MLEYNLGVSTEMTYEIKRSDTDFFSDDLD"
BASE COUNT          869 a         1147 c          984 g          952 t
ORIGIN      
        1 tggcccaagg atgggccaca tggccaaaaa aatagcctgc ggctattttt tattgctccg
       61 caacaaaaaa taatttatct taatggcccc ggccaaagga ttggccggat ggccgaaaaa
      121 acagcctccg cttcgctgcg gctatttttt attgcttcgc aataaaaaac aacttcattt
      181 ctatggccgt ggcccaagga tgggccacat ggccaaaaaa atagcctgcg gctatttttt
      241 attgctccgc aacaaaaaat aatttatctt aatggccccg gccaggggac tggccggatg
      301 gccgaaaaaa tagcctgcgg ctatttttta ttgctcttgc cgctccattc ccgtccgcct
      361 ccccggtggg cggacggttt tattataaca ggtctgcgat gccgcgaaaa gcgccctttc
      421 tctttttgca tctcgcgccg ccgaatcccc cactctataa agaaaagagg cgttttgccg
      481 tgcagctcgg cttgaccatc ccgctccagc gctatctgcg cgctccccgg ccgcccgcgc
      541 ccccagcggc agaacggctg ttctgctggg acctgcaccg cattctgctg cgcggcgagg
      601 gcagcctgct ggcggtccac tgcgccagcc gctttgccgt ggtggtcttt ggcctcagcg
      661 ccgtggactg ggcgtcgctg gacggcgtcg taccggcgca gatttgccgc ggcctgctgg
      721 atgcggattt gccgcaggcg ccggtgcgcg cctatcttga gcgctgcggg cccccctgct
      781 tcacccgcac ccacggccga cgggaggtgg cctttctcaa ccgcgcctgg gaggatgtga
      841 tggcctgcga cttcgccctc gaccgcgccc tgccggggca gcccctgctg gagcgggcgg
      901 tcaacgccct gccccgccgc tgtgccggac aagagggcct cgcgtcctcc cgcgagcgcc
      961 tgctgcagct gttggtctga gcgtgcacgc aaaaggcggc ggacagcacc ctgcccgccg
     1021 ccgcggcggc ccggcccctt tcaaggcgtt atcactgctt ttgaaaaaca tccagcgcat
     1081 gacttgtgac gcccaacagg tctttgcgct cgcaggtggc gaagtgatag ttcaaataca
     1141 gatcaaatgt tttgtcatcc atcgcatcga ttgcctctcg tatcaacagg gcgcagccgt
     1201 cggcggcgac atagtgcagc ctttttacaa aaaatgcgga catcaactcg tcaacgtcct
     1261 ctttccgcac aagctcgaac aaatctttcg gctgggattc tgcggcgaac gtctcggggt
     1321 ccagcaatcc gttctttata tattcagcga cattgacact gccgcgcttg aaaccctcat
     1381 cgagcaaaca gccgtctgag atcacatacg cggcaaaaat gacgccgccc ggctttgtca
     1441 cgcgaatcgc ttcccggata gcccggcgct tgctctcttg gtcgtacaaa tggtaaagcg
     1501 gccccaacaa aagggtgacg tcatatcgat tgcttgaaaa agcggacagg tccaaagcgt
     1561 tgccctgcag gacggagacc tgttcttccg ccgacgtgtt tttgcgaaaa atttcgatat
     1621 tgtgctccac cagttccacc gcgtcaaccg catatccctg acgcgctaaa gcgtgggaat
     1681 accgccccgt tccggcccca atctccagga tacggctgcc gggtttcaaa tatttctcta
     1741 tataacgcat agtggtgaaa aattcaaccc tcccatgctt tgaggtaagg cggctgtctt
     1801 catcataatg atggtaaaaa tcgactaaat agggatttgt ttccatgcta tgccctctcc
     1861 ttttccgttt cctgttctac ataagcggac agaaactccg tcagcatttt ttcagaatcc
     1921 ctcctgccca acgacagttt gccggtcgcc gtcaaaacgg cgatgccgtg cgtgtaaaga
     1981 aacaggtcta aaaatattct tttcccgccc tccggccgca cgccgatcat ctcggaaaat
     2041 tcgtttgctt tttcctcatt ttcaagctct ttataaaaat cctccgcctt tgccatatcc
     2101 agctccatat cattgataaa caacagcttg aacaggttcg tttcctcttt ggcgaactcg
     2161 atataggaga ggggccaaaa caaatagggc ttgatcgttt tcgcctttct gtactctgca
     2221 gcataccggc tgtagtattc aaaggcaaaa tccagaaaat cttttttgag ctcttccatg
     2281 tttttgtaac aggtaaaaat ggggcgtgtg gaacattcca acctgcccgc gacacttctt
     2341 gcgttgacag cttcaaaccc catttcccgt gtaattttca agacggtgtt taaaatcatg
     2401 tcttttgtta ttttgggttt cggcggcatc ggcggtttcc tctttcttgt tatatgcaac
     2461 taatgttgtg caacaattgt tacatataaa accgtttttg tcaatagaaa aacttccctg
     2521 cgcaggaact ttgtgcggaa ccgccggggc ggagcgtccc gggcggagtt ttgagaaaca
     2581 ggcgggagaa cggagggccc tggacttggc cggagctctg caaaagggga gagaaagaag
     2641 agagaaaggg gacggccggc cgcgggcgct tttgaaaccg cccgcaaagc agaaagccgg
     2701 gccgcacctg gcggcggagg aacgggggcg caaaaacaaa aacagcgaca tggagcttgg
     2761 ctccctgccg ctgcaaagcg tccggcgcaa tttgggctca atcaagatcg tcgctgaaaa
     2821 aatccgtgtc actgcgtttg atttcatagg tcatttcagt cgaaacgcca aggttatatt
     2881 ccagcatcaa tttcgcaagc atatgcccgt cgacaagcac gacctttgtg gtatgctgct
     2941 tgtccgcata ctccctcgct tctcttgtga agcgcgccgt tgtgataaac agccccttgc
     3001 tcgccccctg gccggcaagt gccccaacga atctttgaat ttcagggcgg ccgattgttt
     3061 ggagcagttc ccatctcttc gcctggatgt aaatcaagct gaagcccaat ttatcctcgc
     3121 ggataatgcc gtcaatcccc tcgtctccgc tttgcccgac gaccataccg gcattctcca
     3181 gagaaccgcc atagcccatg cgctctaaaa gtttgacgac cagcgcttca aagaatgccg
     3241 gcgtctgctt catgatttcc gccaacaggt catcataaag cgcatcgttg attttttggt
     3301 aggcatcgtc cagaatgtcc tgcgggctgt cgcctgatag ggcgggaagc gactgcgcgg
     3361 ggacggagct gtcgctccct ataaattgac aaaaagaatc gaattgttcc aaataatgat
     3421 tatccaaaac agggggatcc tccacaagca cctgttttcc tgtctgcgta agctgatatg
     3481 cgccgcggga cgggctgtca atcaagccgg cctttttgag gtaggtgcgc gtccaaccga
     3541 ctcggttatc aaaaatcgga cgatgcccgc tgggcagcag gatatgccgt tcctcatcag
     3601 aaatctgaaa ttttgctgca agaagctctt tcatttcctt tgacttatgc acttgcatgt
     3661 ccgccagaca gtccataaag gcccgataca tctcgtcata tttgggaatc gccatcccgt
     3721 ctcatcctgc tttcctgatg attttttctt cctctgtgaa acctgtttct ccaaaaaaat
     3781 tccatcgccg tgaatccgcc cggcgtccgc cccgctcttc ggcccaaggg gcggcccccg
     3841 catatgggat agggccaaag ctttcgaaac cgcccgtttt ttctctcttc tccctcaatg
     3901 atacgcagtt ccgcggtcaa atgtcaaccg cagaacaaaa atctcggaac gt
//
LOCUS       DBMDYA010000198         3946 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5357_length_3946_cov_1.281925, whole genome shotgun sequence.
ACCESSION   DBMDYA010000198 DBMDYA010000000
VERSION     DBMDYA010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5357_length_3946_cov_1.281925"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="ACZPFK_06035"
     CDS             complement(<1..530)
                     /locus_tag="ACZPFK_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1405903.1"
                     /translation="MTAVNYKPTLRACYLGYVTQAVVNNLAPLLFALFQTDFSIPLEK
                     IGQLILLNFGTQLLVDLASVKLVDRLGYRFSACAAHLTSAVGLALLGLLPRLLPDPYL
                     GLALAVMVCAVGGGLIEVLISPIVEALPGDAKASAMSLLHAFYCWGQVLVVLLSTLFL
                     RIWPAAWPLLTALWGLL"
     gene            complement(527..1579)
                     /locus_tag="ACZPFK_06040"
     CDS             complement(527..1579)
                     /locus_tag="ACZPFK_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1405904.1"
                     /translation="MNFHNTRQLKKLNTEQVRAALRACGTATKAWVAHLTSLSIATCG
                     TILDEMVASGEAFESAPAPSDGGRPARRFTYNPDFQLILALVVEHSGSQLRYLSRVVN
                     AVGEVKEERSGSPASAVDYALLRALAEQAVRRYPAIRAVGIGLPCVVQDGVVEAGVLP
                     ALENIPLGKRLAEDLGLEVLVGNDMLFTTYGFYARQEGDCGDTVAVLYFPKNACPGSG
                     AVVDGQLLRGASNFAGEISFLPVGLTCDEQAALFSGGDPAQQIQVIARMAECVIALLN
                     PSTLAVCGDAVQPGMLPEIRALCAGQIPEKHLPRMEYLEDVESCYLSGVVSITMESLR
                     YRLQSDPAKSEYRRFL"
     regulatory      1818..1951
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            2049..2657
                     /locus_tag="ACZPFK_06045"
     CDS             2049..2657
                     /locus_tag="ACZPFK_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1405905.1"
                     /translation="MSEKRSELVFLSPFSAAYWRSAAGVVKKVRMLTLTALFIALRVV
                     VGLFPIPLGDNLHIFFGFFINGLGSAIYGPLLGLVSGFCTDILGFIVHPTGAFFPGYV
                     LTSMLGSFFYGLFFYRARISGLRVLLAKLVVNLGINVGLGALWSAILYGKGYYYYLAK
                     SLVKNIGMWPLEAVLLFLFFKAILPVTAHAGLTPKQDGVKLW"
     gene            complement(3145..>3946)
                     /locus_tag="ACZPFK_06050"
     CDS             complement(3145..>3946)
                     /locus_tag="ACZPFK_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1405906.1"
                     /translation="LRKPRTDIIAVTMPCFGTTSRTKSNAELLCKALGVTFNTVDIGE
                     AVRVHFRDIGHSEAERDVVYENAQARERTQVLMDIANDMNGLVVGTGDLSELALGWAT
                     YNGDHMSMYGVNASVPKTLVRHMVAYAADTAREAGLREVLLDILDTPVSPELLPADGE
                     DIAQRTEDIVGPYDLHDFYLYYVVRFGFSPRKIYRLALLAFEEQFSADEILKWLKIFY
                     RRFFAQQFKRSCLPDGPKVGSVSLSPRGDWRMPSDACAALWMKELEEL"
BASE COUNT          745 a         1218 c         1273 g          710 t
ORIGIN      
        1 agcaggcccc acagcgccgt caacagcggc caggcggcgg gccatatccg caaaaacagg
       61 gtgctcagca gcaccaccag cacctgcccc cagcagtaaa aggcgtgcag caggctcatg
      121 gcgctggcct tggcgtcgcc gggcagggcc tccacgatcg ggctgataag cacctcaatc
      181 agcccgccgc ccaccgcgca gaccatcacc gcgagggcaa ggcccagata ggggtccggc
      241 agcagacggg gcagcagccc cagcagcgcc agcccgacgg cgctggtgag atgggccgcg
      301 caggcggaaa agcggtatcc cagccgatcc acaagcttga ccgaggcgag gtccaccagc
      361 agctgggtgc cgaaattgag caggatcaac tgcccgatct tttccagcgg gatggaaaaa
      421 tccgtctgga agagggcgaa cagcagcggc gcgaggttgt tgaccacggc ctgggtgaca
      481 tagcccagat agcaggcccg cagggtcggc ttgtaattca cggccgtcat aaaaacctcc
      541 ggtactctga ttttgccggg tcgctctgca gccggtaacg caggctttcc atggtgatgg
      601 agacgacgcc ggacagatag cagctctcca cgtcctccag atactccatc cggggcagat
      661 gcttttcggg gatctgccct gcgcagagcg cgcggatctc cggcagcatc ccgggctgca
      721 ccgcgtcccc gcagaccgcc agggtggagg ggttcagcag cgcgatgacg cactccgcca
      781 tgcgggcgat gacctgaatc tgctgtgccg ggtcgccgcc gctgaacagc gccgcttgtt
      841 cgtcacaggt gagccccacg ggcagaaagg agatttcgcc cgcaaagtta ctggccccgc
      901 gcagcagctg cccgtccacc accgccccgc tgccggggca ggcgttcttg gggaaataga
      961 gcaccgccac agtgtcgccg caatcgccct cctggcgggc gtaaaagccg taggtggtaa
     1021 agagcatgtc gttgcccacc agcacctcca gccccaggtc ctcagccagt cttttgccca
     1081 gcgggatatt ttccagcgcc ggcagcacgc ccgcctccac caccccgtcc tggacgacgc
     1141 agggcaggcc gatccccacc gcacggatcg ccggatagcg acgcacggcc tgctctgcca
     1201 gggcgcgcag cagcgcgtag tcgacggcgc tcgcaggcga gccggagcgc tcctccttga
     1261 cctcgcccac cgcgttgacc acccggctga gataacgcag ctgcgagccg ctgtgctcca
     1321 ccaccagggc caaaatcagc tggaagtcgg ggttgtaggt gaagcggcgc gccggccgcc
     1381 ctccgtcgga gggggcgggc gcggattcaa acgcctcgcc gctggccacc atctcatcca
     1441 ggatggtgcc gcaggtggcg atgctcagcg aggtgagatg ggcgacccag gccttggtgg
     1501 cggtgccgca ggcccgcagc gcggcccgca cctgctcggt gttgagcttt ttcagctgtc
     1561 gcgtgttgtg aaaattcatc ggtgacacaa aaacgaccta ctttcaataa gttttgtcaa
     1621 aagtaatgac gaaagtatac ctctttttgc tgttgctgtc aacagaaaaa acggcggcgg
     1681 gctttgtcaa aaagcgaggc gccgttcccg tcgcaaaagg agggaaaaaa cggcgcaaac
     1741 gggccgcgca gaaagcggtg gacaaatgtg tcggggcgtg ctataatcgt ggcattcggg
     1801 ggccgcaggc cccatcacac agagtagaga gctgcgcgtc aggccggggt cacccggccg
     1861 ccagtgccca ccggacgggg agttgccggc gggacgaaag gggaacggtg tttccgcatc
     1921 ccccgcggtg cagccctcgc atcccgctgt accaaaaaac gagggccgtc gccgggagcg
     1981 cgcggcctga gcagagcatc cgccgggtgc gttggtacaa accgatgcac agaggagggt
     2041 gtttttttat gtctgaaaag agaagcgaac tggtctttct gtccccgttt tcggcggcct
     2101 attggcgcag cgccgcaggg gtggtcaaaa aggtgcgcat gctgaccttg accgcgttgt
     2161 ttatcgcgct gcgggtggtg gtggggctgt tccccatccc gctgggggat aacctgcaca
     2221 tctttttcgg ctttttcatc aacggcctgg gcagtgccat ctacggcccg ctgctggggc
     2281 tggtgtccgg cttttgcacc gatatcctgg gctttatcgt ccatcccacg ggggcgtttt
     2341 ttcccggcta tgtgctcacc agtatgctgg ggtctttctt ttacggcctg tttttttacc
     2401 gggcgcgcat cagcggcctg cgggtgctgc tggcaaagct ggtggtcaac ctgggcatca
     2461 acgtggggct gggcgctttg tggagcgcta tcctctacgg caagggctac tattattacc
     2521 tggccaagag cctggtcaaa aacatcggca tgtggccgct ggaggcggtg ctgctgttcc
     2581 tctttttcaa ggccatcctg ccggtgaccg cccacgcggg cctgaccccg aaacaggacg
     2641 gcgtgaagct gtggtgagtt cttttcaccg ccgcggcctc tcttttggaa gggcagaaag
     2701 ggacgaaaaa aacaagaaaa ggcggcctgc atgggcgcgt ttttgcagga gaacatgggc
     2761 cgcgggaaga ggagagtgcg cgcggagcgc cgtcacatcg atccccgcag ccttagagga
     2821 accttgcgtt tccactccgt aaaatgcctg cacgggcggg caaatatcgc cccaggcgtt
     2881 cggtgcaaat tttcccgctg tacatcacag tttttccccg gtgtgtgcgg cggtttcgga
     2941 gcggcaattg ggaagaaagc gcagtgcaaa aaaggttgac gattccgctg gatcgccaac
     3001 cttttttcat tcatttgtaa gtgtgccggg gcgccacagc cgccggctta aaatcaatat
     3061 tttctacctc agcgcgccca gagggccgtc tcttttattt ttaaagggag agcgcgggcc
     3121 gaaaggcgga cggcgagggt cgcttcaaag ctcctccagc tctttcatcc acagggcggc
     3181 gcaggcgtcg ctgggcatcc gccagtcgcc gcgcggcgag agcgagacgc tgcccacctt
     3241 gggcccgtcg ggcaggcagc tgcgcttgaa ctgctgcgca aaaaagcggc ggtaaaagat
     3301 cttcagccat ttcaggatct cgtcggcgct gaactgctcc tcaaaggcga gcagggccag
     3361 ccgataaatt ttgcggggtg aaaagccgaa ccgcaccaca tagtagaggt aaaagtcgtg
     3421 caggtcgtag gggcccacga tatcctcggt gcgctgggcg atgtcctcgc cgtccgcggg
     3481 cagcagctcc gggctgacgg gggtgtcgag gatgtccagc agcacctccc gcagaccggc
     3541 ctcccgggcg gtgtcggccg cataggcgac catgtgccgc accagggttt tgggcaccga
     3601 ggcattgacg ccgtacatcg acatatggtc gccgttgtag gtcgcccacc ccagcgccag
     3661 ctcggacagg tcgccggtgc ccaccaccag gccgttcatg tcgttggcga tatccatcag
     3721 cacctgggtg cgctcgcgcg cctgggcgtt ctcgtagacc acatcccgct cggcctcgct
     3781 gtggccgata tcgcgaaagt gcacccgcac ggcctcgccg atatccaccg tgttaaaggt
     3841 gacccccagc gccttgcaga gcagctcggc gttggatttg gtgcgggagg tggtgccgaa
     3901 gcagggcatg gtgacggcga taatgtcggt gcggggcttg cgcagc
//
LOCUS       DBMDYA010000199         3938 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5364_length_3938_cov_1.758144, whole genome shotgun sequence.
ACCESSION   DBMDYA010000199 DBMDYA010000000
VERSION     DBMDYA010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5364_length_3938_cov_1.758144"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="ACZPFK_06055"
     CDS             <1..1024
                     /locus_tag="ACZPFK_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1405907.1"
                     /translation="YGDTQSNEEAAATEAERLITEVGVSALMGCYQSAVTASVQTVCE
                     KYKVPLAITCSTGDILTEGQKPYSVTIHEQNQDTTKTHAQFIQDMNEKEPGCIKTAAI
                     LYQNDDWGQSLSDQWKERLADIGIEIVVDEVFAQDVTDLTTVVTKLVEKQPDVVLNAC
                     YFQSMVLLTQTFIGMGYEPKAMLCSSSGETDNDFIPTVGNNADGFFTVSGWGEDVLLA
                     MPDKQWIGEEYKERYDGEHYTAESAAGWAAAMVICEGLEAAGTDDSDALNTAIRGLVI
                     PTDAWWNIYPYEIAFDPVTGKNMNAIPVMGQFQDTKIKLIYPESIQAEGTELVFPGYE
                     LSPLNK"
     gene            1110..1820
                     /locus_tag="ACZPFK_06060"
     CDS             1110..1820
                     /locus_tag="ACZPFK_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405908.1"
                     /translation="MHLLELNQVSVSYDAVLALQKCSIYIDEGEMISIVGSNGAGKTT
                     ICKAISGVLDIRGGEVFFDGKDITKVPGYDRVELGIIQCPEGRHLFPDMTVMENLEMG
                     AYCRRGRRDVEENLRYVLGLFPLLEERRGQAARTLSGGEQQMLAIGRSLMSDPKLLIL
                     DEPSLGLSPVNVQLIFSKLGEIRARGTTILLVEQNVEKSLRNSSRGYALENGRIVLTG
                     TGEELLANEDLKRVYLGM"
     gene            1935..2984
                     /locus_tag="ACZPFK_06065"
     CDS             1935..2984
                     /locus_tag="ACZPFK_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405909.1"
                     /translation="MNLKEIAQAAGVSQSTVTMVLHDRKGVGAATKERVSALLEANGY
                     AIKRQIPPSVVAEPKKVIFIKYKRHSLMVDGNPEYISRLMDEVEQCCNKAGYELVFKV
                     ANAETLPDVFEKVNNEPFEGSILLGTEFSNEDRPLLKNVRKPIVIIDNDLVKLSVTSV
                     STHTWNSMRGKVEYLLRRGHKQIGYIRNARPTSICTECFRGYLNVMESYGLEVHDEYV
                     YDVEPTFDGAYMSMKKLLGQGIDLPSALVSNSDCLALGAMKALQEYGVRVPENVSVIG
                     GDNIASCTMHSPKLTTCDAHPCEIGKWAVKLLQEQIENPAGPVIRLRVETTIIERESV
                     CDFSDCIPFKKVEDR"
     gene            3052..>3938
                     /locus_tag="ACZPFK_06070"
     CDS             3052..>3938
                     /locus_tag="ACZPFK_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1405910.1"
                     /translation="MKIRSKGAEGVLLTSLSALLYGTLPVFAGFAYAAGSNAETFNFY
                     KSVWALPVLAILLAARKQSLLLPWRMALWAAAAGVLGKGITSLLLYSSYNYVSSGVAT
                     TLHFMYPLFAALLGRIVFHTRLPGYKWATLLAATAAVCLFIDPADGRKGMIGVLCAVA
                     SGAVYAAYILVVDKSGASAVDPMVFAFYLAASGAIFSLIYGVGTGTLHFFPSAEAHLY
                     TAGAAVCTSVLGAAFFQQGIRLLGGASAAFFSLLEPVASCVFGAVFLSERVGWRAAAG
                     ILLILASVMVMIWLDARRAG"
BASE COUNT          859 a         1028 c         1181 g          870 t
ORIGIN      
        1 ttatggggat acccagtcga acgaggaggc ggcggccacc gaggcggagc gtctgatcac
       61 cgaggtcgga gtctccgcgc tgatgggctg ctatcagtcg gcagtcacgg cgagcgtgca
      121 gaccgtctgc gaaaagtata aggtgccgct cgcgattacc tgttctaccg gcgatatcct
      181 caccgagggg cagaagccct attcggtcac gatccacgag cagaaccagg atacgaccaa
      241 gacccacgcg cagttcatcc aggatatgaa tgaaaaagag cctggatgta tcaagaccgc
      301 cgccatcctg taccaaaacg acgattgggg ccagtccctg agcgaccagt ggaaagagcg
      361 cctggcggac atcgggatcg agatcgtggt ggacgaggtc tttgcacagg acgtcaccga
      421 tttgaccacc gtggtcacaa agctggtgga aaaacaaccc gacgtggtgc tgaacgcctg
      481 ctatttccag tccatggtgc tcttgaccca gacctttatc ggtatgggct atgagcccaa
      541 ggcgatgctc tgttcctcct ctggcgagac ggacaacgac tttatcccca cggtgggcaa
      601 caatgccgac ggtttcttta ccgtttccgg ctggggtgag gatgttttgc tggcaatgcc
      661 ggacaagcag tggatcggtg aagagtacaa agagcggtac gacggcgagc actataccgc
      721 agaatcggca gcgggctggg cggcggccat ggtgatttgc gagggcctcg aggctgcggg
      781 cacggatgat tccgacgcgt tgaacacagc gatccggggg cttgtcattc ccaccgacgc
      841 ctggtggaac atctatccgt atgagatcgc gtttgacccg gtcaccggaa agaacatgaa
      901 cgccatcccg gtcatgggac agttccaaga caccaagatc aagctgatct accctgaatc
      961 tattcaggcg gagggcaccg aactcgtttt tccgggttat gagctttccc cgctgaataa
     1021 atagaaaaag caccggactc ttttgtgggc tgcacccggc tgcagcccac aaaagggggt
     1081 gcagcgtggc aggaagagag gaaacgcata tgcatctgtt agagttgaac caggtctctg
     1141 tttcatatga tgccgttctg gcgctgcaaa aatgctccat ctatatcgac gagggggaga
     1201 tgatcagcat cgttggctcc aacggcgcgg gcaagacgac gatctgcaaa gctatttccg
     1261 gggttttgga tatccggggc ggcgaggtct tctttgatgg caaggacatc acaaaggtgc
     1321 ccggctacga ccgcgtcgag ctcgggatca tccaatgccc ggagggccgt catcttttcc
     1381 ccgacatgac agtgatggaa aatctggaga tgggggccta ttgcaggcgc gggcgcagag
     1441 acgtggagga gaatctcaga tatgtactgg ggttgttccc cctgctcgaa gagcgccggg
     1501 ggcaggcggc gaggaccctt tcgggcggtg aacagcagat gctcgccatc ggccgttccc
     1561 tgatgtccga ccccaagctt ctcatcctcg acgagcccag cctgggcctt tcccctgtca
     1621 atgtacaact gatcttttcc aagttggggg agatccgggc ccgcggtacg accatcctgc
     1681 tggtggagca gaacgtagaa aaatctctgc gcaattccag ccgcggatat gcacttgaaa
     1741 acggcaggat cgtactcacc ggcacaggcg aagaactgct cgcaaatgag gatctgaagc
     1801 gtgtgtatct gggaatgtag ccgctttgag aggagtgaaa aatttaataa attttagtgg
     1861 tttttaattg acaccccatt tttttcgtga tactctatcc ataatataaa atatagatta
     1921 tggcggtatg cgggatgaat ttaaaagaaa tagcgcaggc agcgggggta tcacaatcta
     1981 cggtgacaat ggttctgcat gaccgcaagg gggtcggcgc ggcgacaaaa gagcgcgtca
     2041 gcgccctgct ggaggcaaac ggatacgcga taaaacgaca gatccccccc tccgtggtgg
     2101 cagagcccaa aaaagtcatt tttatcaaat acaagcgcca ctcgctgatg gtggacggca
     2161 accctgaata catttcgcgt ttgatggatg aagtggaaca gtgctgcaac aaagcgggct
     2221 atgagcttgt gtttaaagtc gcaaatgccg aaacgctgcc tgacgttttt gaaaaagtaa
     2281 ataacgagcc ttttgaagga agtattcttt tgggcacgga gttcagcaac gaagaccggc
     2341 ctttactaaa aaacgtccgc aagccgatcg ttatcatcga caatgatctg gtaaagctct
     2401 ctgtcaccag tgtctccacc catacctgga actcgatgcg ggggaaagtg gagtatctgt
     2461 tgcgcagagg acacaagcag atcggctata tccgcaatgc gcgccccacc agcatctgta
     2521 ccgaatgttt ccgcggttat ctcaacgtga tggaatccta tgggctggag gtgcacgacg
     2581 agtatgtcta tgatgtcgaa ccgacctttg acggcgctta tatgagcatg aagaagctgc
     2641 tgggacaggg gattgatctg ccctctgcgc tggtgtcaaa ttctgattgc cttgcccttg
     2701 gggcaatgaa ggccctgcag gaatacggag tccgtgtacc ggaaaatgtc tctgtcatcg
     2761 gcggcgataa tatcgccagc tgtaccatgc attcccccaa gctgaccacc tgtgacgccc
     2821 acccttgcga gatcggaaaa tgggcggtca aactgctgca ggaacagatc gaaaacccgg
     2881 ccggccccgt cattcgcctg cgggtggaga cgacgatcat cgagagggaa agcgtgtgtg
     2941 atttttctga ctgcatcccg tttaaaaaag tggaagaccg ctgacgggac aggtcctttt
     3001 aaaaggcagt gtgcgttgcg cactgccttt tccttttaac ggaggtcata aatgaagatc
     3061 cgctccaaag gggcagaggg ggtgctgctc acttctctct ccgccttgct gtacggcaca
     3121 ttgccggtct tcgccggctt tgcctatgcg gcgggcagca atgcggagac gttcaacttt
     3181 tataaaagcg tatgggcgct tccggttttg gcaattttgc tggccgcccg caagcagtcg
     3241 cttttgctgc cctggcgcat ggctctgtgg gcggccgcgg cgggcgtgct gggcaagggg
     3301 atcacgtcgc tgctgctcta ctccagctat aattatgtga gcagcggggt cgccacgacg
     3361 ctgcacttta tgtatccgct ttttgccgcg ctgttgggac gaatcgtttt tcacacccgc
     3421 ctgcccggat acaagtgggc cactcttttg gccgccacag cggcggtctg ccttttcatc
     3481 gacccagcgg acgggaggaa agggatgatc ggcgttttat gtgcggtggc gtctggggcg
     3541 gtgtatgccg cctatattct ggtggtggat aaaagtgggg catccgctgt ggacccgatg
     3601 gtctttgcgt tttatctcgc agccagcggc gcgatatttt cactgattta tggtgtcggc
     3661 accgggacgc tccacttctt tccctctgcc gaggcacacc tctacacggc gggggccgcg
     3721 gtgtgcacct cggttcttgg ggcggcgttc tttcaacagg ggatccgcct gctgggaggc
     3781 gcttcagccg catttttcag cctgctggag cccgtggcga gctgtgtgtt cggcgcggtc
     3841 ttcctctctg aaagggtcgg ctggcgggcg gccgccggca ttctgctcat cctggcttcg
     3901 gtcatggtga tgatctggct ggatgcccgc agggcggg
//
LOCUS       DBMDYA010000200         3928 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5380_length_3928_cov_1.764829, whole genome shotgun sequence.
ACCESSION   DBMDYA010000200 DBMDYA010000000
VERSION     DBMDYA010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_3928_cov_1.764829"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="ACZPFK_06075"
     CDS             complement(<1..966)
                     /locus_tag="ACZPFK_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1405911.1"
                     /translation="MTQREREILQIIRDDPMISQYDIAKKLGITRSAISAYLNTMYKK
                     GILKGRGYIINEELKPLLIGPSHVDIRSVCVDRNAGQSTPIYTAAQTQITYGGPIKNI
                     AEYLMEFGISSRAMFAVASDSFGASFLSACRQKGIDSSGSVVVPNSTSPVYMEMVDQD
                     QQLIASSFATSDLTTAITPAHLAGQSPLLRGTNLIALHDSVPPESVTAIHTISPSANL
                     FLVSSSAEYSLRHVPLFPLFCCAMLHYDIAFEIVSQVGDLGRMLDYTPDQPLPIVRAL
                     AKLGLSNFYLLLDHSSVCHCDGKTLTVHIAQLPAGAGGSLAYLYSD"
     gene            1375..2316
                     /locus_tag="ACZPFK_06080"
     CDS             1375..2316
                     /locus_tag="ACZPFK_06080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405912.1"
                     /translation="MSTYLSCGNIMSDIIVQPSGEKSTRHMGGPAFFALSGIRLWEDD
                     CALVTHTGADWSEDYEKWLDAHHLSKAGVRVDAEHVTCYQITYDENGRGVPDPQKSFS
                     FYGDEAMGYLKTHPSDIKKALTPGVKGLYMAQGADRVVWRQLAALKEEFHFKMMWELE
                     YFNTGKDLDRVLEALPLADAWSLNHTEASGLFGIPIADDEKMLRRLQELPVPYTFYRV
                     GKRGAFLVSKDGAWFCESIDIGPYVDQTGCGNSSTGAMMYALFSGHTPQEALVMANLA
                     SGYNCLQYGPYPLYTDEVRRQALGLRGEYLKKVKQVL"
     gene            2424..3599
                     /locus_tag="ACZPFK_06085"
     CDS             2424..3599
                     /locus_tag="ACZPFK_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1405913.1"
                     /translation="MKKVLALILAAAMAFGLVACGGGGDSSSTPAAGSGNAASTASTA
                     GSGEKDGTLLYYCAFIGDFGLGDMGNRASLAAAEKYNLEYKLVEYGTDSSVAVNSLRD
                     ALETSTYDYVVASSWYISDVMKDIAPMYPDTTFILFDTSPTGDFEGLDNVYGITFGQN
                     EGGFLVAVYSALMTKTGKIGCSLRSDTPILNDFGTGWLAGYKYAAKEMGLDVDMVFSY
                     IGEDSVQSTYESCNVLFDNGADYVWSVAGTNVLGACQAAEEKGGLEAGYGVIGVDYDQ
                     WTYFKEVGDAVGYENLVTSMLKNIEPCVALIFESVIEGKGDIKPGNSFYGVATGGTGL
                     AVNDNYTENTPKEVQDKITELSEKIASGEIDVPSYFDFNSYEAYAAYRDNPDADFAA"
     gene            3683..>3928
                     /locus_tag="ACZPFK_06090"
     CDS             3683..>3928
                     /locus_tag="ACZPFK_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1405914.1"
                     /translation="MSKELLRMEGITKIYGNGFMANKDVTFSVSEGEIHALIGENGAG
                     KTTLMKILFGEEDCQNGKIYINGEEAHIANPLDAIAKG"
BASE COUNT          849 a         1148 c         1154 g          777 t
ORIGIN      
        1 atcgctgtag agataggcca ggctgccgcc ggcccccgcc gggagctggg caatgtgcac
       61 tgtcagggtc tttccgtcac agtggcacac cgaggagtgg tcgagcagca gatagaagtt
      121 ggagagcccc agctttgcca gcgcgcgcac gatgggcagg ggctggtccg gcgtgtagtc
      181 cagcatccgc cccaaatcgc cgacctggct gacgatctca aaggcgatat cgtagtggag
      241 catggcgcag cagaagagcg gaaaaagcgg gacatgccgc agcgaatact ccgctgagga
      301 ggagacgaga aacagattcg cgctgggcga gatggtgtgg atggcggtga ccgactccgg
      361 gggcacggaa tcgtggaggg cgatgaggtt cgtcccccgc agcagagggc tctgccccgc
      421 caggtgggcc ggcgtgatgg cggtggtcag gtccgaggtg gcaaaggagc tggcgatcag
      481 ctgctggtcc tggtctacca tctccatata gacgggggag gtggaattgg gcaccacgac
      541 cgagccgctg ctgtcgatcc ccttttgccg gcaggcgctg agaaaggagg cgccgaaaga
      601 gtcggaggcc accgcgaaca tggcgcggga ggagatcccg aactccatca ggtactcggc
      661 gatgttcttg atggggccgc cgtaggtgat ctgggtctgg gccgccgtgt agatcggggt
      721 gctctgcccc gcgttccggt ccacgcacac gcttcggata tccacatggc tggggccgat
      781 caacaggggc ttgagctcct cgttgatgat atagccgcgc cctttcagga tgcccttttt
      841 atacattgta ttcaaataag ctgagatcgc gctgcgggtg atccccagct ttttggcgat
      901 gtcgtactgg gagatcatcg gatcgtcccg gatgatctga aggatttccc tctcccgctg
      961 tgtcattgtc cacactccaa aattaacttt tatcctcccg gcgcgcggcc agaaccgccg
     1021 cgctctcccg gcagaccggg cgccgccttt attttaccat aaaaccggct tttgcgcact
     1081 tgtgcccgac tctttatttc ttttagggat ttgcccggtg aaactgatcc cccctccccg
     1141 cctttcggcc gcctccgccg cccaaaggca gccttttcag cgcatttttc tggtctttcc
     1201 aaagattctt tccgttttaa aaaataccac tttttacatt ttttagagaa aattgacaaa
     1261 aatataaaaa aattatgata taaaaagtca atattaataa ttaatttgat atgatattat
     1321 gattatgctt taaaaataca gtaaatcatt tattgcattt gagaggtcac ggttatgtcc
     1381 acttatcttt cctgcggcaa tatcatgtcg gatatcatcg tccagccgag cggggaaaaa
     1441 agcacccggc acatgggcgg gcccgctttt tttgcgctgt ccggcatccg cctgtgggag
     1501 gatgactgcg cgctcgtgac ccacaccggc gccgactggt ctgaggacta tgaaaaatgg
     1561 cttgacgccc atcacctctc aaaggccggc gtgcgggtgg acgccgaaca cgtcacctgc
     1621 taccagatca cctacgatga aaacggacgg ggcgtccccg acccccaaaa atccttttcc
     1681 ttttacggcg acgaggcgat gggatacctg aagacccatc cctccgacat caaaaaagcc
     1741 ctcacgccgg gggtgaaagg cctttatatg gcgcaggggg ccgaccgggt ggtgtggcgc
     1801 cagctggccg cgctgaaaga ggagttccac ttcaagatga tgtgggagct ggaatacttc
     1861 aacaccggca aggacctaga ccgtgttctg gaagcgcttc cccttgcgga cgcctggtct
     1921 ctcaaccaca ccgaggcaag cgggctgttc ggcatcccca tcgccgacga cgaaaaaatg
     1981 cttcgccggc tgcaggagct gccggtcccc tacacgtttt accgggtggg caagcgcggc
     2041 gctttcctcg tcagcaagga cggggcatgg ttctgcgaat ccatcgatat cggcccctat
     2101 gtggaccaga ccggctgcgg caacagctcc accggggcga tgatgtacgc gctcttttcc
     2161 ggacacaccc cgcaggaggc gctggtcatg gcgaatctcg cctccggcta caactgcctg
     2221 caatacggcc cctaccccct gtacaccgac gaggtgcgtc ggcaggccct ggggctgcgc
     2281 ggcgaatacc tgaaaaaagt gaagcaggtg ttgtgagccc catccattct tttgtacctt
     2341 atcccgtgcg cgtaggcggg tcataaaggt tgccgcgggc cctccgcggt aaagtagaaa
     2401 ggcaaatcaa aggaggagaa ctaatgaaaa aagtattggc attgatcctt gctgcagcga
     2461 tggctttcgg cctcgtcgcc tgcggcggcg gcggtgattc cagcagcacc cctgctgctg
     2521 gcagcggcaa cgccgccagc acggcgagca ccgcgggcag cggtgagaaa gacggcaccc
     2581 tgctgtacta ctgcgcgttt atcggcgact tcggtctggg cgacatgggc aaccgcgcgt
     2641 ccctggctgc ggccgaaaag tacaacctgg aatacaagct ggtcgagtac ggcaccgaca
     2701 gctcggtcgc cgtcaacagc ctgcgcgacg cgctcgagac ctcgacctac gactatgtcg
     2761 tggcctccag ctggtacatc tccgacgtca tgaaagacat cgcccccatg tacccggata
     2821 ccacctttat cctgttcgac acctccccga ccggcgactt tgagggcctg gacaatgtct
     2881 acggcatcac gttcggccag aacgagggcg gcttcctggt ggcggtctac tcggccctga
     2941 tgaccaagac cggcaagatc ggctgctcgc tgcgctccga caccccgatc ctgaacgact
     3001 tcggcaccgg ctggctggcc ggctacaagt acgccgccaa agagatgggc ctggacgttg
     3061 acatggtctt ctcctacatc ggcgaggatt ccgtgcagtc cacctatgag tcctgcaacg
     3121 tgctgttcga caacggcgcc gactatgtct ggtccgtcgc cggcaccaac gttctgggcg
     3181 cctgccaggc ggccgaggag aagggcggcc tggaagcggg ctacggcgtt atcggcgttg
     3241 actacgacca gtggacctac ttcaaagagg tcggcgacgc tgtcggttac gagaacctgg
     3301 tcacctccat gctgaagaac atcgagccct gcgtggccct gattttcgag agcgtcatcg
     3361 agggcaaggg cgacatcaag ccgggcaaca gcttctacgg cgtggccacc ggcggcaccg
     3421 gcctggctgt caacgacaac tacactgaga acaccccgaa agaggtccag gacaagatca
     3481 ccgagctgtc tgagaagatc gccagcggcg agatcgacgt tccctcttac ttcgacttca
     3541 acagctacga ggcgtatgcc gcttatcgcg acaacccgga cgctgatttc gcggcgtaat
     3601 cccactaaaa attaaatcag ttcaatcgct gagcgggcgg ggtaactccc gcccgctcag
     3661 tcattaaagg ggaggcaaac gaatgtctaa ggaacttctc agaatggagg gcatcaccaa
     3721 gatctacgga aacggtttca tggcgaacaa ggacgtcacc ttttccgtga gtgagggcga
     3781 gatccacgcg ctgatcggcg aaaacggcgc cggcaaaacg accctcatga agatcctttt
     3841 tggtgaagaa gactgtcaga acggcaagat ttatatcaac ggcgaggagg cgcatatcgc
     3901 aaacccgctg gacgccatcg caaagggc
//
LOCUS       DBMDYA010000201         3915 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5403_length_3915_cov_1.828789, whole genome shotgun sequence.
ACCESSION   DBMDYA010000201 DBMDYA010000000
VERSION     DBMDYA010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5403_length_3915_cov_1.828789"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="ACZPFK_06095"
     CDS             <1..959
                     /locus_tag="ACZPFK_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405915.1"
                     /translation="HLYTAAPRAAQAAPAAVPAGPELAPAPRRRRRWVLPVVLVLSVL
                     LLAGGGFAWYTWDAYTDLQDAWGYKNYAQVVLCYRRAPWVETWAGSEPKLYSEAMVHV
                     ENYELEEAIGLFQQLNGYADSEKQIQLAQNYLKAEGYPERSIVSKYTLFQELGNYLDS
                     QKKVAALQPQIYKKGVAKVENEEFYDAEGYFEVLPQDYEDTGFYTILLQNYEDVIRTG
                     DPDYCYAFAVGCQVAGMDVPYILTDTYLYEFIQGYWATSNGWWFEKTGEYYNTNIGLY
                     GTYYFEKSGMYTERGRVVEFRFLDADHMRLIYNGYYYDMTRY"
     gene            1408..2979
                     /locus_tag="ACZPFK_06100"
     CDS             1408..2979
                     /locus_tag="ACZPFK_06100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1405916.1"
                     /translation="MKRKKKRIAALLASLAFLLCASVAGAAGGTPSVVLRARDGVVRV
                     FSYQEDGFFTGTGFALSNNSSGAMILTNYHVVDGCSAYELYYDGNGPVALEIVAVSEV
                     QDLCVLRTAHKLKGLKPLPLANGVSSGEAVYTLGYPGGADDLSLQMALTKEKMTVSNG
                     IVSAIQDSNLVGNYMRPVKLVQTNADINHGNSGGPMLNEKGQVVGVNTLTMNDFSVSG
                     INGAVHVNEVRSFLDVQGIRYQTSSALVWEIAAIAAVLAALVLAAFLLRRKKQKALRR
                     QAALPAGAADSAGWSAAAGPARPAGPEGTGSPAAAAGQSKFFSAAQPVREIEEKEIDG
                     QISLFDDPAPEATLPPTAASAADDLSAEGKETARKKKGGRRGFLAAAALALMLLAVGG
                     FGFYTWDVYTDIESAWGYQNYEQVAACYRRAPWVEVLSGSEPRRYSEAMLKAQAHEME
                     EAVALLEELGDYGDAKEQIELAQGYQKVLSIPATNLVGRYSGIRDIGTWGITWIALRM
                     QNFFWRRFTTRLSSG"
     gene            2943..3404
                     /locus_tag="ACZPFK_06105"
     CDS             2943..3404
                     /locus_tag="ACZPFK_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405917.1"
                     /translation="MEEIYNKVVERLSTGNMGGNKAYLEVLPNDYRDIAVYKEFASAY
                     DRLLSHPSSDTCLSFCYGCDALGFQLPIAIPNDYLDYFLYGRWETSTGWYFELDEKYY
                     DTNIEEISGSIFIRPNVIYIDGKSVFATVNILDTDHITMICEGRSYSMTRK"
     gene            complement(3517..3765)
                     /locus_tag="ACZPFK_06110"
     CDS             complement(3517..3765)
                     /locus_tag="ACZPFK_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HIR1405918.1"
                     /translation="MDDKALMEDLLLVVKGNCGLYLDGTIESATKSVHKAFDDVLEDT
                     LMIQNELYTKMADKGWYPTETAEQKQINKVVKQFAPKN"
BASE COUNT          814 a         1043 c         1196 g          862 t
ORIGIN      
        1 tgcatttgta cacggccgcg ccccgggcgg cacaggctgc tcccgcggcg gtcccggccg
       61 gccccgagct ggcccccgcg ccccggcgca ggcgccgctg ggtcctgccc gtcgtgttgg
      121 tcctgtcggt gctcttgctg gcgggcggcg gcttcgcctg gtatacctgg gacgcctaca
      181 ccgacctgca ggacgcctgg ggctataaaa attacgccca ggtggtgctt tgctaccgtc
      241 gcgcgccctg ggtggagacc tgggcgggca gcgagccgaa gctgtacagc gaggcgatgg
      301 tgcatgtgga gaactatgag ctggaggagg cgatcgggct ttttcaacag ctgaacggct
      361 atgcggattc tgaaaagcag atccagctgg cgcagaacta tctcaaggcg gagggctatc
      421 ccgagaggag catcgtctca aaatacaccc tgtttcaaga gctggggaac tatctggaca
      481 gccagaaaaa ggtggcggcc ctgcagccgc agatctataa aaaaggggtc gcaaaggtag
      541 aaaatgaaga attttacgat gcggagggat attttgaggt cctgccccag gactatgagg
      601 ataccgggtt ctataccatc ctgctgcaaa attatgagga cgtcatccgc accggcgacc
      661 cggattattg ttatgcgttt gcagtgggat gccaggtggc gggcatggat gtgccgtata
      721 ttctgaccga cacctatctg tatgaattca tacaggggta ttgggccacc tccaacggct
      781 ggtggtttga aaagacaggg gagtattaca acaccaacat cggcctctac ggcacctact
      841 attttgaaaa aagcgggatg tataccgaga ggggaagagt ggtggaattc cgctttctcg
      901 acgccgacca catgaggctg atctataacg gctattatta cgacatgacc cgctattaaa
      961 aattcctctg ccgcagccag gcacccgccc gcctcgggcg gcataccata actcaggcgc
     1021 gcgccttgga atatgggcgt cccgccggtg ccctggcagc ggcagggccg tcaccagagc
     1081 ggggccggag gttttcctcc ggccccgctt tgcatcggcg gcgtttcatc aagggcggcc
     1141 gcaggctgtt tttcggccca ccgagggccg gtcgaggctg cttgagagaa tttatttttg
     1201 cgggtctttg cccattttga ggagctgttc aaaaggtttg gggccgtggc cccggtctct
     1261 acagccgctt tccggctttg caggcgaggc gggaaaaaca aaaacttacc ttgtccatcc
     1321 aatggcgtct ggcaaaaaag gcttcgacct gttgcaaatt tcgcaaaaat gctatactgg
     1381 aaacaaaaaa gggggaaaac agcacccatg aaaagaaaaa agaagcggat cgccgcattg
     1441 ctggcgtcgc tggccttttt gctgtgcgcc tctgtggcgg gcgcggcggg gggcacgccg
     1501 tccgtggtgc tgcgtgcccg ggacggcgtg gtgcgggtgt tttcttatca ggaggacggc
     1561 tttttcaccg gcacgggttt tgccctttcc aataacagct cgggggcgat gatcctcacc
     1621 aactatcatg tggtggatgg ctgctcggcg tatgagctct actacgacgg caacgggccg
     1681 gtggcgctgg agattgtggc cgtgtccgag gtgcaggacc tgtgcgtgct gcggacggcg
     1741 cacaagctca agggcctcaa gccgctgcct ttggcaaacg gcgtttccag cggcgaggcg
     1801 gtttacaccc tggggtaccc gggcggggcc gacgacctat cgcttcaaat ggccttgacc
     1861 aaagagaaga tgacggtgag caacggcatc gtcagcgcca tccaggattc caacctggtg
     1921 ggcaattaca tgcgcccggt caagctggtg cagaccaatg ccgacatcaa tcacggcaac
     1981 tccggcggcc ccatgcttaa cgaaaagggc caggtcgtgg gggtgaatac cctcaccatg
     2041 aatgattttt cggtcagcgg catcaacggc gcggtgcatg tgaacgaggt gcgcagcttt
     2101 ctcgacgtgc agggcatccg ctatcagacc agttctgcgc ttgtctggga gatcgccgcg
     2161 attgcggcgg tgttggcggc gctggtgctg gcggcctttt tgctgcggcg caaaaaacaa
     2221 aaggccctgc gccgccaagc cgccctcccg gcaggcgcgg cggattctgc cggctggagt
     2281 gccgcagccg gccctgcccg cccggcgggg ccggagggaa caggttctcc ggctgctgcg
     2341 gcagggcaga gtaaattttt ctctgcggcg cagccggtgc gggagatcga agaaaaagag
     2401 atcgacgggc agatcagctt gtttgacgat cctgcccccg aagcgaccct gccgcccacc
     2461 gctgcgtctg cggccgacga tttgagcgca gagggaaaag aaacggcgcg caaaaagaaa
     2521 ggcgggcgcc ggggcttttt ggcggcggcc gcgctggcct tgatgttgct ggccgtcggc
     2581 ggttttggtt tttatacctg ggatgtttac acggacatag aatccgcctg gggctatcaa
     2641 aactatgaac aggtggccgc ctgttatcgc agggcccctt gggtggaagt actctccggc
     2701 tcggagccca ggcgctacag cgaggcgatg ctgaaagctc aggcccatga gatggaagag
     2761 gccgtcgcct tgctggaaga gctgggagac tatggggacg caaaagagca gatcgagctg
     2821 gcacaggggt accaaaaggt tttgtcgatc ccggcaacca acctggtggg gcggtatagc
     2881 ggtataaggg atatcgggac ctggggaatt acctggattg ccctcagaat gcagaacttc
     2941 ttttggagga gatttacaac aaggttgtcg agcggctgag caccggaaat atgggcggga
     3001 acaaagctta tttagaagta cttcccaatg actatagaga tatcgccgtc tataaagaat
     3061 tcgcttctgc ctacgatcgg ctgttgagcc accccagttc ggatacctgc ctctcctttt
     3121 gttatggatg tgatgctttg ggttttcagt tgcctatagc aatcccgaat gactatttgg
     3181 attatttttt atatgggaga tgggagacct ccacagggtg gtattttgag ctggacgaaa
     3241 aatattatga caccaatatt gaagaaatca gcggttctat ttttatccgg ccgaatgtca
     3301 tctatataga tggtaaaagt gtttttgcaa ctgtaaacat cctagatacc gatcacatca
     3361 ccatgatttg cgagggccgc tcttacagca tgaccagaaa gtgatctacc gcaacaaaat
     3421 accgcccgcc gggttttcca ccggcgggcg gtattttgtt gaaaactttt taaaacgcac
     3481 ggcagccctc aggcaaaccg cgttttactg cggcgctcag tttttgggcg cgaactgctt
     3541 gaccaccttg ttgatctgct tttgctcagc ggtctcggtg gggtaccagc ccttgtcggc
     3601 catcttggtg tagagctcgt tctggatcat cagggtatcc tccagcacgt cgtcgaatgc
     3661 cttgtgcacc gacttggtgg cggattcgat ggtgccgtcc agatacaggc cgcagttgcc
     3721 cttgaccacc agcaacaggt cttccatcag cgctttgtca tccattttca ttctcctcct
     3781 tagcccttgt ccacagtgtc gtagaacacc tcaaaatgct cgcaaaagcg gttttcgagc
     3841 tttttgacgt agttctgcag gctcttatcg gtcagttggg ctgccggctc ctggcatttc
     3901 tgctgcatgt gctgc
//
LOCUS       DBMDYA010000202         3906 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5418_length_3906_cov_1.695926, whole genome shotgun sequence.
ACCESSION   DBMDYA010000202 DBMDYA010000000
VERSION     DBMDYA010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5418_length_3906_cov_1.695926"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="ACZPFK_06115"
     CDS             <1..320
                     /locus_tag="ACZPFK_06115"
                     /inference="COORDINATES: protein motif:HMM:NF023227.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DinB/UmuC family translesion DNA polymerase"
                     /protein_id="HIR1405919.1"
                     /translation="DLSRELAEAAVALVTANWDLRQPIRAITVTAMGLTDEATPRQTS
                     LFGEPGQDPRHRRRARLEQALDGLRDRFGPDAVAAAGVLGSDLGLMENKARPRAEEEE
                     PDP"
     gene            553..840
                     /locus_tag="ACZPFK_06120"
     CDS             553..840
                     /locus_tag="ACZPFK_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR1405920.1"
                     /translation="MMVKHIVFWKLREDCDKDAATREIAGKLEALVGVVPGLTRAEVR
                     RAYTGDYDTVLYSELESREAAEAYQTHPAHLEAKKTVHGYAVGRAACDFEV"
     gene            complement(1216..1458)
                     /locus_tag="ACZPFK_06125"
     CDS             complement(1216..1458)
                     /locus_tag="ACZPFK_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1405921.1"
                     /translation="MTVGAAVKERILDLCQEHNLSINKLCTTSGVPQSTINNLVNGRN
                     NSITVSTVKKLCDGLGITLEEFFHSEIFQMLEQEVK"
     gene            1728..2042
                     /locus_tag="ACZPFK_06130"
     CDS             1728..2042
                     /locus_tag="ACZPFK_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405922.1"
                     /translation="MAFPYHILLDRVDLQNIITHAQGFDLSGSDEREMQVRDKEAYDG
                     MQRQFLALVGEARLEDALDAAMGYLSVREDIMLALGMKMGARLQLQLLSGEDLDFFRA
                     PK"
     gene            2318..>3906
                     /locus_tag="ACZPFK_06135"
     CDS             2318..>3906
                     /locus_tag="ACZPFK_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405923.1"
                     /translation="MRTKMKLLVALLAAAVAGIALVLLAGVLDLRPAARPYTVEGPVR
                     ALRAPMAGPGGALATVTPEMTAQEATAALQTLVDGAVSTGANTLLFEVQSSAVAGVYY
                     DDKAFSPVNSALDPLKTLCELARQAGVQVIAVVEPFAAGPADKSTVKGTLASEFYSET
                     LRYGGGLWFNPLNVRCVDRLAAVYARLVKHYPVAGVVFSGLEFGAEGDALAQGLAVLG
                     EKMGKAAGEKALGLTFDGASAPLSEAQAAALTAGGFGLLLPRGIDEAALGQWRDACPR
                     AQVLPSAPANDPEGFSGLLRTLSLKNKLPGAVLGDLAAAVQNPADVGLMVSYFCEPVA
                     VASPGVSTALATSYPPDGSTVTSETVVILGTSDPTRPLAVSSGSTILRAQGGSFAVEV
                     PLQVGRNEFTLTQEGGGTAAVTVIRRDPASGGGGSTGPKPDTALDDSLAGRYVEVTAQ
                     LASTLNDPGNDDAIAMTVKQGARISIRGTVETLRSGKITTAYLLSSGDYLLAANAQLV
                     EDGAPAALSAPEQSVSARGDLS"
BASE COUNT          671 a         1218 c         1366 g          651 t
ORIGIN      
        1 ccgacctgtc gcgggagctg gcggaggcgg cggtggccct ggtgacggcc aactgggacc
       61 tgcgccagcc catccgcgcc atcacggtca cggccatggg cctgaccgac gaggcgaccc
      121 cgcgtcagac cagcctgttc ggcgagcccg ggcaggaccc gcgccaccgc cgccgggcca
      181 ggctggagca ggcgctggac ggcctgcggg accgcttcgg ccccgacgcg gtggcggcgg
      241 caggggtgct gggcagcgac ctgggcctga tggagaacaa ggcgcgcccc cgcgccgaag
      301 aggaggagcc agacccctaa aggcccctga ggcggcggcc cgcgccgcgc tcacctggcc
      361 gctctttctc tttgtcggga cgcggcttcg gccgcgccgg aaaaggggaa tccgccgcaa
      421 aagcggcgga ggctgtgcct cagcgcaggt ttgcgcggcc tgcgccgccg cccttgcccg
      481 gggcccggcg cggaaagcga aaaaacagac aaccacccgg cgcaaggatg ccgggatgaa
      541 aggagtaaaa gtatgatggt aaagcatatc gtgttctgga aactgcggga ggactgcgac
      601 aaggacgccg ccacccgcga gatcgcgggc aagctggagg cgctggtggg cgtggtgccg
      661 ggcttgacca gggcggaggt gcgccgcgcc tatacgggcg actatgacac cgtgctgtac
      721 agcgagctgg agagcaggga ggccgccgag gcctaccaga cccacccggc ccacctggag
      781 gctaaaaaga cggtgcacgg ctacgccgtg ggccgcgcgg cctgcgattt cgaggtctga
      841 gtcgccgcgc gggcaaaagg gcgcgggcat ggccgcacgc gccgcggcgc ggctgcgctg
      901 gacaagaaaa aggaaacgca ttttgggcgt cttgtccccc gcagcggcgg agcgcggagg
      961 cgcgggaggc gcaggagcgc gggaactctg aaaaagtgaa gacaaaaagc gcccgggcct
     1021 gtggaaaaga ggcccgggcg cttttcattt ttgggattcg gatggggcat cggcggggtg
     1081 ttccctctca ggcccgctcc aggggcaacg gggccgccac gcggccgcaa acagggcctc
     1141 acggcagact gtccccaaag tttcagggct gggggacaaa gactttcagc accgcctcga
     1201 tcgcggggaa gtcctctatt tcacttcctg ctccagcatc tggaatatct cggagtgaaa
     1261 aaattcctcc agagttatgc ccaggccgtc gcaaagcttt tttacagtgg aaaccgtaat
     1321 gctgttgttg cggccgttaa cgaggttgtt gatggtagac tgcggaacgc cacttgtcgt
     1381 gcacagcttg ttgatcgaaa ggttgtgctc ttggcataag tcaagtattc tctcttttac
     1441 tgcggctccg acagtcatat tcttcccccc ttttcaaaaa gagtactgtt ttcttcttga
     1501 aaaggttaac ccttttgagt taaaataact caaaagagtt aaaaatcgct tcttctgctt
     1561 ttagtttgca gttactttta agatggaatc cccaaaaatc gtaaaacact ttaatcacga
     1621 ggcctttttc gccaaggccc gttttttcta tgccctctct tttaaaatgg cggcccacaa
     1681 tttattttca aacagtactt ttacagatga aagggtaggt gagtgaaatg gcttttcctt
     1741 atcatatcct gctggaccgg gtggacctgc agaacatcat cacccacgcg cagggctttg
     1801 acctgtcggg cagcgacgag cgggagatgc aggtccggga caaggaggcc tatgacggca
     1861 tgcagcggca gttcctcgcc ctggtggggg aagcgcggct ggaggacgct ctcgacgccg
     1921 cgatggggta tctgtcggtg cgggaggaca tcatgctggc tttgggcatg aagatggggg
     1981 ccaggcttca gctgcagctg ctcagcgggg aggacctcga ctttttccgc gcccccaaat
     2041 aatctcgttt cggcgtaaaa agggcccgcc cggcaggatc gtctgacaag acgggggccg
     2101 ggcccggcaa aaagcggcgg gagagggccg cgccccgccg ctttttgtcg gtgcgctccg
     2161 ctgcgagccg gcgcctctct tcatttttcg gcgcttcttt catacatttt acagtgcgcc
     2221 gccccgtgcg gatatgctat actggtaaaa atgaaaatgt tgtcgaaacg gcgcggcggc
     2281 cgggcccctg ggcaagccgc cggagggagg gactttgatg aggacgaaga tgaagctgct
     2341 ggtggccctg ctggccgcgg cggtggcggg cattgcgctg gtgctgctgg cgggcgtgct
     2401 ggacctgcgg cccgcggccc gcccctatac ggtggagggg ccggtgcggg cgctgcgcgc
     2461 acccatggca gggcccggcg gcgcccttgc caccgtgacc cccgaaatga ccgcgcagga
     2521 ggccaccgcc gcgctgcaga ccctggtgga cggcgcggtc tccaccgggg ccaacacgct
     2581 gctgtttgag gtgcagtcct ccgctgtggc gggggtgtat tacgacgaca aggcgttttc
     2641 acccgtcaac agcgccctgg acccgctgaa gaccctctgt gagctggcgc ggcaggcggg
     2701 ggttcaggtg atcgcggtgg tggagccctt tgccgccggg cccgcggaca aaagcaccgt
     2761 caagggcacg ctggcgtctg agttttacag cgagacgctg cgctatggcg ggggcctctg
     2821 gttcaacccg ctcaacgtgc gctgtgtgga ccggctggcc gccgtctacg cgcggctcgt
     2881 caagcactat ccggtggcgg gggtggtctt ctccggcctg gagttcggcg cggagggcga
     2941 cgccctggcg caggggctgg cggtgctggg ggaaaagatg ggcaaggccg ccggagaaaa
     3001 ggccctcggc ctgacctttg acggcgcgtc ggccccgctg agcgaggcgc aggccgcggc
     3061 cctgacggcg ggcggcttcg gcctgctgct gccccggggc atcgacgagg cggcgctggg
     3121 gcaatggcgc gacgcctgcc cgcgggcgca ggtcctgccc agcgccccgg ccaacgaccc
     3181 ggagggcttt tcggggctgc tgcgcaccct ttcgctgaaa aacaagttgc ccggcgcggt
     3241 gctgggcgac ctggccgcgg cggtgcagaa ccccgcggac gtggggttga tggtcagcta
     3301 tttctgcgag ccggtggcgg tggccagccc gggggtctcc acggcgctgg cgaccagcta
     3361 tccgccggac ggctccaccg tgaccagcga gacggtggtc atcctgggca ccagcgaccc
     3421 cacccggccg ctggcggtct cctccggcag caccatcctg cgggcgcagg gcggcagctt
     3481 tgccgtcgag gtgccgctgc aggtggggcg caacgagttc accctcaccc aggagggcgg
     3541 cggcacggcg gcggtgaccg tcatccgcag ggacccggcg tccgggggcg gcggctccac
     3601 cgggccgaag cccgacaccg cgctggacga cagcttggct ggccgctatg tggaggtcac
     3661 cgcccagctc gcctccaccc tgaacgaccc gggcaacgac gacgccatcg ccatgacggt
     3721 caagcagggg gcccgcatct ccatccgggg cacggtggag acgctgcgca gcggcaagat
     3781 caccacggcg tatctgctct ccagcggcga ctatctgctt gccgccaacg cccagctggt
     3841 ggaggacggc gcccccgccg cgctgtcggc gcccgagcag agcgtgagcg cccgcggcga
     3901 cctgtc
//
LOCUS       DBMDYA010000203         3883 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5454_length_3883_cov_1.531465, whole genome shotgun sequence.
ACCESSION   DBMDYA010000203 DBMDYA010000000
VERSION     DBMDYA010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5454_length_3883_cov_1.531465"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(75..845)
                     /locus_tag="ACZPFK_06140"
     CDS             complement(75..845)
                     /locus_tag="ACZPFK_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1405924.1"
                     /translation="MPTWDAAQYLKFERERTQPAVDLAQRIGLQAPARLLDVGCGPGN
                     STAVLRRLYPDAELLGIDSSPEMITAARRAHPDLRFQLCDVREEITLPGPAFDVVFSN
                     ACLQWVPGHRALLPRLMALLRKGGVLAVQMPVNYDEPIHRILRGLAAASPWKEKLGAP
                     RGMFVLGEEDYFDLLSEIAADFALWRTTYFHRIASHAELMDWYRGSGLRPYLQALSPH
                     DGAVFEEEVLARLKKAYPLQKNGEILLRFPRLFFTAAR"
     gene            1404..3302
                     /locus_tag="ACZPFK_06145"
     CDS             1404..3302
                     /locus_tag="ACZPFK_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1405925.1"
                     /translation="MSRAIGEILRRYRRLFLVLFDVVAVAAAYIGTWMLTLQGSDLLG
                     DGMVGMAAMASPYKKYFSLMASSCFLFVSCYIIIYAFMGMYDSLWRYAEIIEFFRVCV
                     ASFLAVTAFVTLSQLIFTERRIPMAVYLMSAMFATILTLMARLTYRMYRNTKIKSNRK
                     QRRRVLLIGAGDAASMLLHEINRNPSDEMNIICAVDDSPQKVGRTILGVQIMGTTADI
                     PELVERCEIETILLCIPTIEENDKRRILSICSKTNCNLRIMPDLVKLIADGRDLSSRI
                     RDVKVEDLLGREVIEMDPATNTLISGKVVMVTGGGGSIGSELCRQIAAAGPSKLVIVD
                     IYENNAYSIQQELIRKYGDQLDMEVQIASVRDSKKVDRLFAQYRPALVFHAAAHKHVP
                     LMETSPEEAVKNNVFGTYNVALAAEKYGAERFVLISTDKAVNPTNVMGATKRICEMIV
                     QTIAETSKTDFVAVRFGNVLGSNGSVLPLFKEQIAEGGPLTVTDPDIVRYFMTIPEAV
                     SLVLQAASMSGKGNIFVLDMGEPVRILDLAENLIKLAGFIPYRDIDIVFTGLRPGEKL
                     FEELTMDEESVHKTANKKIFVGAPLKMDKANFFTHLARLKEIAYSNDSKRLLAFVSEM
                     VPTFDHEI"
     gene            3316..>3883
                     /locus_tag="ACZPFK_06150"
     CDS             3316..>3883
                     /locus_tag="ACZPFK_06150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1405926.1"
                     /translation="MYPVVKRALDFVCSLLGVVLLSPLLLVLALLVKCTSPGPVLFRQ
                     KRVGRNKTYFEILKFRSMRADTPRDVPTHLLQNAENAITPVGRFLRKTSLDELPQLFN
                     ILLGQMSVVGPRPALWNQDDLIAERDKYGVNALTPGLTGWAQVNGRDELPIPVKAELD
                     GYYAAHISFGLDVKIFFRTIANVLFGKGI"
BASE COUNT          758 a         1168 c         1213 g          744 t
ORIGIN      
        1 gatgccgtcc ctcccgtttt ttatagtgca aaatggagcg cctcccggct ttgcgccggg
       61 ggccggaaac aggctcagcg cgccgccgta aaaaagagcc gtggaaaccg caggaggatc
      121 tcgccgtttt tctgtagcgg atacgctttt ttgaggcgcg ccagcacctc ttcttcaaac
      181 accgcgccat catgtgggga gagagcctgc aaatagggcc gcagcccgct gccgcgatac
      241 cagtccatca gctccgcatg agaggcgatg cggtgaaaat aggtggtccg ccaaagcgcg
      301 aaatccgcgg cgatctctga gagaagatca aaataatcct cctcgcccaa cacgaacatc
      361 ccccgcggcg cgcccaattt ttctttccag gggctggcag cggcgagccc gcgcaggatg
      421 cgatggatcg gctcgtcata gttgaccggc atctgcaccg ccagtacgcc gcccttgcgc
      481 agcagcgcca tcagccgggg cagcagggcg cgatgcccgg gcacccattg gaggcaggcg
      541 tttgaaaaga caacgtcaaa ggccgggccg ggcagggtga tttcttcccg cacatcgcag
      601 agctgaaagc gtagatcggg gtgggcccgg cgggcggcgg tgatcatttc cggcgagctg
      661 tcgatgccca ggagctccgc gtccgggtag aggcggcgca gcactgcggt gctgttgccg
      721 ggcccgcagc ccacgtcgag cagccgggcc ggggcctgca gcccgatgcg ctgtgccaga
      781 tcgacggcag gctgggtgcg ctcccgctca aatttgagat attgcgccgc gtcccaggtc
      841 ggcatgttcc atctctcctc tccgattaaa cctcaaagga atgttccgct tcattgtacg
      901 gcgcccgcgc gggcttgtca acgacagagg agaagtgcaa atttcttttc cgcggcggct
      961 ggcattgccc ttgcaatggg ggcaaggatg tcttttcaga aagtggcgcc tgggccctgt
     1021 tatgacggat tttggccaag agcgtcgtgc ggggggaaaa gcagagaaaa agcgcggcgg
     1081 accggcccgg caggggagcg gctccgccgc gctttttcgg gacctgcttc cccggcggcc
     1141 gctgaaagtg tggccggacg cgccggcgct gcggcgctgt ataggcaggg ggcggggggc
     1201 caatactgaa ttgagccgca tgcagggccc cgtcgctctc aatcaggatt tgattattgt
     1261 cgcaagatgt tgtaaaatgg atgaaagata aaagcgcggg gaaaggcggc tgccgccgtc
     1321 cgcgcgtcaa gagtcgaaat tcaagtgatg gaagcgggcc tcacgggcga tcccgcttcc
     1381 atcatttaga gattggtgat acgatgtcaa gagctattgg cgagatcctg cgccgttacc
     1441 gcaggctgtt tctcgtcctg ttcgacgtgg tcgcggtcgc agccgcctat atcggtacct
     1501 ggatgctcac cctgcagggc agcgacctgc tgggggacgg catggtgggc atggcggcca
     1561 tggccagccc ctataaaaag tacttcagcc tcatggcctc cagctgtttt ctgttcgtca
     1621 gctgctacat catcatctat gcctttatgg gtatgtacga cagcctgtgg cgctatgccg
     1681 agatcatcga gttctttcgc gtctgcgtgg cgtcttttct ggcggtgacg gcatttgtca
     1741 ccctctctca gttgatcttt accgagcggc gcatccccat ggcggtctat ctgatgagcg
     1801 ccatgttcgc caccatcctc acgctgatgg cgcgcctgac ctatcgcatg taccgcaaca
     1861 ccaagatcaa gagcaaccgc aagcagcgcc gcagggtgct gctgatcggc gccggggatg
     1921 cggccagcat gctgctgcac gagatcaacc gcaaccccag cgacgagatg aacatcatct
     1981 gcgcggtgga cgactccccg caaaaggtgg ggcgcaccat tctcggcgtg cagatcatgg
     2041 gcaccaccgc ggatatcccc gagctggtgg agcgctgtga gatcgagacc atcctgctgt
     2101 gcatccccac catcgaggag aacgacaagc gccgcatttt gtcgatctgc tccaagacca
     2161 actgcaacct gcgcatcatg ccggatctcg tcaagctgat cgccgacggc cgcgacctgt
     2221 cctcccgcat ccgcgacgtc aaggtggagg acctgctcgg ccgcgaggtc atcgagatgg
     2281 atccggccac caacaccctg atcagcggca aggtggtcat ggtcaccggc ggcggcggtt
     2341 ccatcggcag cgagttgtgc cgccagatcg ccgccgcggg gccgagcaag ctggtcatcg
     2401 tggatatcta tgaaaacaac gcctactcca tccagcagga gctgatccgc aaatacggcg
     2461 accagctgga catggaggtg cagatcgcct cggtgcggga cagcaaaaag gtggaccggc
     2521 tttttgcaca gtaccggccg gcgctggtgt ttcacgccgc ggcgcacaag catgtgcccc
     2581 tgatggaaac aagcccggag gaggcggtca agaacaacgt ctttgggacc tataacgtgg
     2641 ccctggcggc ggaaaagtac ggcgccgagc ggtttgtgct catctccacc gataaggcgg
     2701 tcaaccccac caacgtgatg ggggccacca agcgcatctg cgagatgatc gtgcagacca
     2761 tcgccgagac cagcaagacg gattttgtgg ccgtgcgctt tggcaacgtg ctgggctcca
     2821 acggttcggt gctgccgctt ttcaaagagc agatcgcgga gggcgggccg ctgaccgtca
     2881 ccgacccgga tatcgtgcgc tatttcatga ccatccccga ggcggtcagc ctggtgctgc
     2941 aggccgccag catgagcggc aagggcaata tcttcgtgct ggacatgggc gagccggtgc
     3001 gcattttgga cctggccgaa aatttgatca agctggcggg ctttatcccc tacagggaca
     3061 tcgacatcgt ctttaccggc ctgcggcccg gtgagaagct gtttgaagag ctgaccatgg
     3121 atgaagaaag cgtgcacaag accgccaaca aaaagatttt tgtcggcgcg ccgctcaaga
     3181 tggacaaggc caacttcttc acccatctgg cccgcctcaa ggagatcgcc tattccaacg
     3241 acagcaagcg cctgctcgct ttcgtcagcg agatggtgcc cactttcgat cacgagattt
     3301 aggagcggtg cccatatgta tcccgtcgtc aaacgagccc ttgattttgt ctgttcgctg
     3361 ctcggcgtcg tcctgctctc cccgctgctg ctggtgctgg cgctgctggt caagtgcacc
     3421 agcccggggc cggtgctttt tcgccagaag cgggtgggcc ggaataaaac ctattttgag
     3481 atcctgaagt ttcgcagtat gcgggcggac accccgcggg atgtgcccac ccatctgctg
     3541 cagaatgccg agaacgccat caccccggtg gggcgctttc tgcgcaagac cagtttggat
     3601 gagctgccgc agctgttcaa tatcctgttg ggacagatga gcgtcgtggg gccgcggccc
     3661 gcgctctgga accaggacga cctgatcgcc gagcgggaca aatacggcgt caacgccctc
     3721 acgccgggcc tgaccggctg ggcgcaggtc aacggccgcg acgagctgcc catcccggtc
     3781 aaggcggagc tggacggcta ttatgccgcc cacatcagct tcgggctgga tgtgaagatc
     3841 tttttcagga ccatcgccaa cgttctcttc ggcaagggga ttg
//
LOCUS       DBMDYA010000204         3879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5464_length_3879_cov_1.781895, whole genome shotgun sequence.
ACCESSION   DBMDYA010000204 DBMDYA010000000
VERSION     DBMDYA010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5464_length_3879_cov_1.781895"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="ACZPFK_06155"
     CDS             <1..455
                     /locus_tag="ACZPFK_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR1405927.1"
                     /translation="KPRPDTLKLSIGYHAGYKVVGMLSFAWPDAYEKAQYAAEILMKK
                     MRRKGLRAEDVHVSYIGLNSLHLGVADMSPAQIEGLNEVVLRIAVRTREKAEAAKLIP
                     EIAPLQLNGPPGASFFGGRPHVQEVIGLWPTLIPRDALQLTTHILEVE"
     gene            457..792
                     /locus_tag="ACZPFK_06160"
     CDS             457..792
                     /locus_tag="ACZPFK_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtuA-related protein"
                     /protein_id="HIR1405928.1"
                     /translation="MPEIYLNQIAHGRSGDKGDTSNVCIFARKPEYYPILQRELTVEK
                     VRAHFGEMVKGEITRYEVPTLDGFNFVMKHALGGGATLSLRLDSLGKSMGSAFLRMKV
                     RVEEGELDV"
     gene            785..1993
                     /locus_tag="ACZPFK_06165"
     CDS             785..1993
                     /locus_tag="ACZPFK_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR1405929.1"
                     /translation="MSDHRALENLTVLDLTRVLAGPFCTMMLADMGADVIKIEVPGGG
                     DDTRGYPPFRENRDGERESLYFANINRNKRGVTLNLKTPEGKALFLRMAERADIVVEN
                     YRPGVMDRLGLGYEQLRRANPRIIYAAVSGFGSYGPYHLRPGYDILAQAMGGMMAITG
                     PAGGGPTRAGSALGDILGGLHLAIGILAAVNARTVTGRGQRVDVSLMDSVIAATENTA
                     VKYLESGRVPPRMGNRYAAVSPYDGFRCKDGVVIVACGNQKLFGKLCTEILGQPQLAD
                     DPRFADMTGRLEHQDELKVLIEGWLADKTMEEAADILLAHGIPAGPILDVSQILSDPH
                     VKEREMFVEMEHPTLGRITVNGCAVKLMGTPAAVRAPAPALGQDNRQVFTALGLSEQE
                     FQALEEKKVF"
     gene            2289..>3879
                     /locus_tag="ACZPFK_06170"
     CDS             2289..>3879
                     /locus_tag="ACZPFK_06170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR1405930.1"
                     /translation="MEEKEKLARLRREFSELDAPSHRARLPQEIWVAAPDGVRLRTVV
                     TLPEGEGPFPTILLRSCYPQAEPEYQLTAQGYAQRGFAFVYQFCRGTGGSEGAWEPNI
                     HEREDGLAAARWLAAQPFVGPCGYLGCSYLAFTGWIMADALPDKFKTLYLTHYGTDRF
                     ASAYQNGLFRHDILTGWAMDNAGFPVKADYLASCRHRPQLTVDEDLWGGRLGWYRDWI
                     THADPHDPYWNEGLWGQLRDIPSRLALPVYIGSGWYDHHLGSTVRTYLALPPHTRAKS
                     VLRIGAWNHNFEPCVDGKPQRHLENSDTASAFRWFRRLLIQGQVPKGGVELYNIGADR
                     WEHFDGLPGAGAKTRELFLSCPDRSLTPRAPQRQAPLRYRYDPDDPVPSHGGDSLFTT
                     RQEVGSLPQPAPGWRPDVLSFVSEPLAAPLHLCGEITAELEVASDCPDTAFTAKLMEA
                     APDGTARNIRGGATTLAYRDAGGAEQPYRPGQRVRATIRMWLVSWTVPAGCRLRLDVS
                     SSNFPEYAAHPNRAGAWARQTG"
BASE COUNT          647 a         1344 c         1302 g          586 t
ORIGIN      
        1 gaaagccccg ccccgacacc ctcaagctct ccatcggcta tcacgcgggc tacaaggtgg
       61 tgggcatgct cagcttcgcc tggccagacg cctacgaaaa ggcgcagtac gcggcggaga
      121 tcctgatgaa aaagatgcgc cgcaagggcc tgcgggccga ggacgtccat gtcagctaca
      181 tcggcctcaa cagcctgcac ctgggggtgg cggacatgag ccccgcccag atcgagggcc
      241 tcaacgaggt ggtgctgcgc atcgccgtgc gcacgcggga aaaggccgag gcggccaagc
      301 tgatccccga gatcgcgccg ctgcagctca acggcccccc gggcgccagc ttcttcggcg
      361 ggcggcccca tgtgcaggag gtcatcggcc tctggcccac cctgatcccc cgcgacgccc
      421 tgcagctgac cactcacatc ctggaggtag aatgagatgc cggaaattta tctgaaccag
      481 atcgcccacg gccgcagcgg cgacaagggc gataccagca acgtctgtat ctttgcccgc
      541 aaaccggaat actaccccat cctgcagagg gagctcacgg tggaaaaggt gcgcgcccac
      601 ttcggcgaga tggtgaaagg ggagatcacc cgctacgagg tgcccacgct ggacggcttc
      661 aactttgtga tgaagcacgc cctgggcggc ggggccaccc tctcgctgcg gttggacagc
      721 ctgggcaaat ccatgggctc cgcttttctg cgcatgaaag tgcgggtgga ggagggcgag
      781 ctggatgtct gaccaccggg cgctggaaaa cctgaccgtg ctggatctga cgcgggtgct
      841 ggccggcccc ttttgcacca tgatgctggc cgacatgggc gccgacgtca tcaagatcga
      901 ggtgccgggc ggcggcgacg acacccgcgg ctacccgcct ttccgcgaga accgggacgg
      961 cgagcgggag agcctctatt tcgccaacat caaccgcaac aagcggggcg tcaccctcaa
     1021 cctgaagacg ccggagggca aagcgctctt tctgcgcatg gcggagcgcg cggacatcgt
     1081 cgtcgaaaac taccgccccg gcgtgatgga ccggctgggc ctgggctatg agcagctgcg
     1141 gcgggcgaac ccgcgcatca tctacgcggc ggtgagcggc tttggcagct acggccccta
     1201 tcacctgcgg ccgggctacg acatccttgc ccaggccatg ggcggcatga tggccatcac
     1261 cggcccggcg ggcggcggcc ccacccgcgc cggcagcgcg ctgggggata tcctgggcgg
     1321 cctgcacctg gccatcggca tcctagcggc ggtgaacgcc cgcaccgtca ccggccgggg
     1381 ccagcgggtg gacgtatcgc tgatggattc ggtgatcgcg gccaccgaaa acaccgccgt
     1441 caaatatctg gagtcgggcc gggtcccgcc gcggatgggc aaccgctatg cggcggtctc
     1501 cccctacgac ggctttcgct gcaaggacgg cgtggtcatc gtggcctgcg gcaaccagaa
     1561 gctgttcggc aagctctgca ccgagatcct gggtcagccc cagctggccg acgacccccg
     1621 ctttgccgac atgaccggcc gtctcgagca ccaggacgag ctaaaagtgc tgatcgaggg
     1681 atggctggcc gacaagacga tggaggaggc cgcggatatc ctgcttgccc acggcatccc
     1741 cgccgggccg atcctggacg tcagccagat cctgtcggac ccccacgtca aagagcggga
     1801 gatgttcgtg gagatggagc accccaccct gggcaggatc acggtgaacg gctgcgccgt
     1861 caagctgatg ggcactcccg ccgccgtgcg cgccccggcg ccagccctgg ggcaggacaa
     1921 ccggcaggtc tttacggcac tggggctctc ggagcaggag tttcaggcgc tggaggaaaa
     1981 aaaggtcttt tgacggcgca cagggcgggg ttcacggccc agttctccct cccggcaggc
     2041 tgccggcctc acaaaagaga gggggcgtcc gcccccttac cggcgctgcc ttaggtcgat
     2101 ggaaagcccg ggtgcggtgg tttcggcctg cccgggcagg gcggccataa aaagaaagcc
     2161 cgccccgcgg cggcgcggcc tggggccgtg ctccccccgc tttcagcgcg gcctggaggc
     2221 gggtgctgcc gcgggcgcgc tccacatccc gccccggcct cgccgtcgat ctcaaagagg
     2281 tgatccgaat ggaagaaaaa gaaaagctgg cccgcctgcg gcgggagttc agcgagctgg
     2341 acgccccctc acaccgggca cggctgccgc aggagatctg ggtagcggcc ccggacgggg
     2401 ttcggctgcg caccgtggtc acgctgccgg agggggaggg gcctttcccc accatcctgc
     2461 tgcgcagctg ttatccccag gccgagccgg aatatcagct caccgcccag ggctatgcgc
     2521 agcgcgggtt cgcctttgtc tatcagttct gccggggcac cggcggctcc gagggggcgt
     2581 gggagcccaa tattcacgag cgggaggacg gcctcgccgc cgcccgctgg ctggcggccc
     2641 agccctttgt cgggccctgc ggttatctgg gctgctccta tctggctttt accggctgga
     2701 tcatggccga cgccctgccg gacaaattca agacgctcta tctcacccat tacggcaccg
     2761 accgctttgc ctccgcctac caaaacgggc tgttccgcca tgatatcctc accggctggg
     2821 ccatggacaa cgcgggcttt ccggtcaagg cggactatct ggcctcctgc cggcatcgcc
     2881 cccagctcac ggtggacgag gacctctggg gcggccggct gggctggtac cgcgattgga
     2941 tcacccacgc cgacccgcac gacccctatt ggaacgaggg gctctgggga cagctgcggg
     3001 acatccccag ccggctggcg ctgccggtct acatcggctc gggctggtac gaccaccatc
     3061 tgggcagcac cgtgcgcacc tatctggccc tgccgcccca tacccgggcc aaaagcgtgc
     3121 tgcgtatcgg ggcctggaac cacaactttg agccctgtgt ggacggcaag ccccagcgcc
     3181 atctggaaaa cagcgatacg gccagcgctt tccgctggtt ccgccgcctc ttgattcagg
     3241 gccaggtgcc aaaagggggc gtggagctgt acaatatcgg cgccgatcgc tgggagcatt
     3301 ttgacgggct ccccggcgcg ggggcgaaga cgcgggagct gtttctctct tgccccgacc
     3361 ggtcgctcac cccgcgcgcg ccccagcggc aggcccctct gcgctaccga tatgacccgg
     3421 acgatccggt gcccagccac ggcggcgact cgctgttcac cacccggcag gaggtgggca
     3481 gcctgcccca gcccgcgccg ggctggcggc cggacgtgct gagctttgtc tccgagccgt
     3541 tggccgcgcc gctgcacctg tgcggcgaga tcacagccga gctggaggtc gccagcgact
     3601 gcccggacac cgcattcacc gccaagctga tggaggcggc cccggacggc acggcgcgga
     3661 acattcgcgg cggcgccacc acgctggcct accgggacgc ggggggcgca gagcagccct
     3721 atcggccggg ccagcgggtg cgcgcgacca tccgcatgtg gctggtctcc tggaccgtgc
     3781 ccgcgggctg ccgtttgagg ctggacgtct cctcctccaa tttccccgaa tacgccgccc
     3841 accccaaccg cgcaggcgcc tgggcgcgcc agaccggcg
//
LOCUS       DBMDYA010000205         3855 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5499_length_3855_cov_1.604688, whole genome shotgun sequence.
ACCESSION   DBMDYA010000205 DBMDYA010000000
VERSION     DBMDYA010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5499_length_3855_cov_1.604688"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="ACZPFK_06175"
     CDS             <1..678
                     /locus_tag="ACZPFK_06175"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405931.1"
                     /translation="HIDFTARPGETTAFIGSTGSGKSTLINLIPRFYDVTGGSITLDG
                     VDIRQLSQKELRDNIGYVPQKGLLFSGTIASNLRYGDERASTDDLEQAAGIAQAKEFI
                     DQKPDGFDEPISQGGTNVSGGQKQRLSIARALVKKAPVYIFDDSFSALDFKTDAALRH
                     ALREKTADSTVLIVAQRISTIMNAEQIIVLDEGRMVGKGTHKQLLESCETYREIALSQ
                     LSKEELA"
     gene            675..2546
                     /locus_tag="ACZPFK_06180"
     CDS             675..2546
                     /locus_tag="ACZPFK_06180"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405932.1"
                     /translation="MSEQNKGARAPRRGPFGGHGPGGPRGMMPGEKAKDFKGTFRKLV
                     RYLSQYHVTILVVAAAAMLSCVFAIAGPKVMAKATNKLFEGVIAMLTGAGGIDFSYIG
                     WIILILVGLYALSCALSYLQGWLMTGVTQKVSYRLRRDISLKINRLPLSYFDGTSTGD
                     VLSRVTNDVDTLSQSLNQSLTQIVTSVTTVLGVLAMMLSISPLMTLVAVLTLPLSLLF
                     ITQIVKRSQPFFVRQQRYLGEANGHIEEMYGGHVVVRAFNGEDRSIETFNEINDKLYN
                     SAWKSQFLSGLMQPLMGLVGNIGYVAICVLGGWLTVKGTISVGDIQAFIQYVRSFNQP
                     INQLANISNVLQQTAAAAERVFEFLDEKEEIPDPEQPVSADKVEGQVLFAHVKFGYDP
                     EKIIIHDFAHIVQPGQKVAIVGPTGAGKTTMVKLLMRFYDVNEGAILVDGYDVRQFTR
                     NDLRGLFGMVLQDTWLYNGTILENIRYGRLDATDEEVYAAARMAGVEHFVHTLPEGYD
                     TVLNEEASNISQGQKQLLTIARAVLSDPKILILDEATSSVDTRTELHIQHAMDRLMKG
                     RTSFVIAHRLSTIKDSDVILVMRDGDIVEVGSHDELMERGGFYAELYQSQFEPEETA"
     gene            complement(2635..3153)
                     /locus_tag="ACZPFK_06185"
     CDS             complement(2635..3153)
                     /locus_tag="ACZPFK_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HIR1405933.1"
                     /translation="MQSVTEDIHFTAGYADPAPWPELDVSRPDRGWAALLAEDYAGAG
                     GETTASLQYIYHHTVTAGYPDVSEALEKISVVEMMHMEKLAKAILALGGDPQFSARGM
                     PWYAQNVAYGGGLGEYLHADLAGEYTAIRCYRAHIEAIEDESVRLLLERVIRDEELHV
                     EVFKRLIAKYGL"
BASE COUNT          691 a         1334 c         1179 g          651 t
ORIGIN      
        1 cacatcgact ttaccgcccg gcccggtgag accacggctt tcatcggctc gaccggcagc
       61 ggcaaatcca ccctcatcaa cctgatcccc cgcttttacg acgtcaccgg cggcagcatc
      121 acgctggacg gggtggatat ccggcagctc agccagaaag agctgcgcga caacatcggc
      181 tatgtgccgc agaaagggct gctcttctcg ggcaccatcg cctcgaacct gcgctacggc
      241 gacgagcggg caagcacgga cgacctggag caggcggccg ggatcgccca ggccaaggag
      301 tttatcgacc agaagccgga cggcttcgac gagcccatca gccagggcgg caccaacgtg
      361 tccggcgggc agaagcagcg cctgtccatc gcgcgggcgc tggtgaaaaa ggcgccggtc
      421 tacatcttcg acgactcgtt ctcggcgctg gacttcaaga ccgacgcggc cctgcgccac
      481 gccctgcgcg agaaaaccgc ggacagcacc gtgctgatcg tggcccagcg catctccacc
      541 atcatgaacg ccgagcagat catcgtgctg gacgaggggc gcatggtggg caagggcacc
      601 cataagcagc tgctggagag ctgcgagacc taccgcgaga tcgcgctgag ccagctgtca
      661 aaggaggaat tggcatgagc gaacagaaca agggcgcccg cgccccgcgc cgcgggcctt
      721 tcggcggcca cgggcccggc gggccccgcg gcatgatgcc gggcgaaaag gccaaggatt
      781 tcaagggcac tttccgcaag ctggtgcgct atctgagcca gtaccacgtg accatcctgg
      841 tggtggcggc cgcggccatg ctctcctgcg tgttcgccat cgcgggcccc aaggtgatgg
      901 ccaaggccac caacaagctg ttcgagggcg tcatcgccat gctcaccggc gccgggggca
      961 tcgatttcag ctacatcggc tggatcatcc tcatcctggt gggcctgtat gcgctgtcct
     1021 gcgcgctgtc ctatctgcag ggctggctga tgaccggcgt gacccaaaag gtctcgtacc
     1081 ggctgcgccg ggacatcagc ctcaagatca accgcctgcc cctgagctat tttgacggca
     1141 cctccaccgg cgacgtgctc agccgcgtca ccaacgacgt ggacaccctg agccagagcc
     1201 tcaaccagag cctgacccag atcgtcacca gcgtcaccac cgtgctgggc gtgctggcga
     1261 tgatgctctc gatcagcccg ctgatgacgc tggtggctgt gctgacgctg ccgctctcgc
     1321 tgctcttcat cacccagatc gtcaagcgca gccagccttt ctttgtgcgg cagcagcgct
     1381 acctgggcga ggccaacggc catatcgagg agatgtacgg cggccatgtg gtggtgcgcg
     1441 cgttcaacgg cgaggaccgc agcatcgaga cgttcaacga gatcaacgac aagctgtaca
     1501 actcggcctg gaaaagccag ttcctgtccg gcctgatgca gccgctgatg ggccttgtgg
     1561 ggaatatcgg ctatgtcgcc atctgcgtgc tgggcggctg gctgaccgtg aaaggcacca
     1621 tcagcgtggg cgacatccag gctttcatcc aatatgtgcg cagcttcaac cagcccatca
     1681 accagctggc aaacatcagc aacgtgctgc agcagaccgc ggcggccgcc gagcgggtct
     1741 tcgagttcct ggacgaaaaa gaggaaatcc ccgaccccga gcagcccgtg tccgcggaca
     1801 aggtggaggg ccaggtgctg ttcgcccacg tcaagttcgg ctacgacccg gaaaagatca
     1861 tcatccatga tttcgcccat atcgtgcagc ccgggcaaaa ggtggccatc gtcggcccca
     1921 ccggcgcggg caagaccacc atggtcaagc tgctgatgcg tttttacgac gtcaacgagg
     1981 gcgcgatttt ggtggacggc tacgacgtgc gccagttcac ccgcaacgac ctgcgcggcc
     2041 tgttcggcat ggtgctgcag gacacctggc tgtacaacgg caccattttg gagaatatcc
     2101 gctacgggcg gctggacgcc accgacgagg aggtctacgc cgcggcccgc atggccgggg
     2161 tggagcactt cgtccacacc ctgcccgagg gctacgatac cgtgctcaac gaggaggcca
     2221 gcaacatcag ccagggccag aagcagctgc tgaccatcgc ccgcgccgtg ctcagcgacc
     2281 cgaagatcct gattctggac gaggcgacca gcagcgtgga cacccgcacc gagctgcaca
     2341 tccagcacgc catggaccgg ctgatgaaag ggcgcaccag ctttgtcatc gcccaccgcc
     2401 tgtccaccat caaggacagc gacgtgattt tggtgatgcg ggacggcgac atcgtggagg
     2461 tgggcagcca cgacgagctg atggagagag gcggctttta cgccgagctc taccagagcc
     2521 agttcgaacc cgaggagacc gcctgagcgc ggtgcccccg cgggccaaaa gcaagacaaa
     2581 gaagaggacc ggatgctttg gcatccggtc ctcttcttta tttttgcccc cgcctcacag
     2641 gccgtacttg gcgatcagcc gcttgaagac ctccacatgc agctcctcgt cgcggatgac
     2701 gcgctccagc agcaggcgca cgctttcgtc ctcaatggcc tcgatgtggg cgcggtagca
     2761 gcggatggcc gtgtactcgc cggccaggtc ggcgtgcagg tattcgccca ggccgccccc
     2821 gtaggccaca ttctgggcgt accagggcat gccccgggcg ctgaactgcg ggtcgccccc
     2881 cagggccagg atggccttgg ccagcttttc catgtgcatc atctccacca ccgagatctt
     2941 ctccagcgcc tcggacacgt ccggataccc ggccgtcacg gtgtgatggt agatgtattg
     3001 cagcgaggcg gtcgtctcgc ccccggcccc ggcataatcc tccgccagca gggccgccca
     3061 gccgcggtcg ggcctgctca cgtccagctc cggccagggc gccgggtcgg cataaccggc
     3121 ggtgaaatgg atgtcctctg tcactgattg catcaaaatc ccctccttgc cccaatacct
     3181 atgcgcgccc cggccctccc tatgccctgc ccgggcctcc ggcgcatacg ggcgtctgcg
     3241 ccgccccctc ccgctcaccg tctccccggc gcggcttcgc tatgccgtct cccccttgtc
     3301 aggggacgca gaaagggcga ggtgcggggc tttctcctat atctgcggcc gcgcatttca
     3361 tggctcccct cccaaaaacg caaggcgtgc gcggcttgga gcttgctccc aatccgcggc
     3421 agcgcgctcc atggcccccc tccccccaaa accggcggcc gcaggccgtc cgagtttttg
     3481 tttttttagc cggcggggca aaactctcat ttttccgacg actgcggcgc cgcccgccct
     3541 ttccggcttc tgcaaggcgg gagtcccctc ccctcgcccg ggaaagcagg acccgcgcgc
     3601 ctttgggccc ccgcgcccgc ccgaagcgcg cctattttct acccatgccc cgtcccgtcg
     3661 tctctgcgcc gccccctcct cctcccgcgg agtgggagcg gtcccctctc gccaggggga
     3721 aagcagaccc gcgcgcgggc aaaagaacgc cgccgggaat cttcccgacg gcattctttc
     3781 tccagctgcg gcgcgatcag ggcttctttt tcagcgggcg gcgcacgccg tactcgtcga
     3841 tggtctcagc gctgt
//
LOCUS       DBMDYA010000206         3845 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5516_length_3845_cov_1.757479, whole genome shotgun sequence.
ACCESSION   DBMDYA010000206 DBMDYA010000000
VERSION     DBMDYA010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_3845_cov_1.757479"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(49..1011)
                     /locus_tag="ACZPFK_06190"
     CDS             complement(49..1011)
                     /locus_tag="ACZPFK_06190"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405934.1"
                     /translation="MKKIIIDTDTGSDDAVALLMALREPSVQVVAFTTVSGNIEVNQA
                     TYNCLQTIEYADTYRPPVYKGASHPLYCAYENADQVHGKDGMGDVGTFKKPTREAEPE
                     FAVDAMLRLLREGDGDIEIFAIGPLTNLAMAILQEPETMKKVPHITIMGGGLPYNNPH
                     TVWAEFNVMCDPEAADIVCSFGVPFTMVTLEACWGDMRFDEADIKGFRELNEAGRFCV
                     DCNCTAIDLARCVHGEGSFDLPDPAAFIPLVRPDLIRTSFESYTRVDLKGEHTRGATI
                     FETPGSSFMKEAFGQKHHEPNSLIVTELDGPGFKQYLWELLDRR"
     gene            complement(1028..1978)
                     /locus_tag="ACZPFK_06195"
     CDS             complement(1028..1978)
                     /locus_tag="ACZPFK_06195"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1405935.1"
                     /translation="MAKKVWIDTDTGSDDAVALVMMLRDPSVEVVGISTVAGNVGLEQ
                     ATKNALMTIGYAAAAQPPVYLGCERPLFHEHRSAVAVHGEDGMGDLGALQPGGQPASE
                     KAVTALLGAAGEGGLEILALGPLTNLALAMMRDPDTMRRVGHITLMGGAYKKGNTGHL
                     AEFNLWEDAEAADYVFTFGVPITMVTIDACRGEARFTEADCARLRAAGSRCADFCVDC
                     NRTLYEANAAYGERYFTLPDAVAAAVLLRPGLVRDAAPSYTRVELKSERAYGATVNDL
                     RPRDRLEFVSKRSVFPPYNCQLVSGIDAEGFKQLLCQLVI"
     gene            2082..2225
                     /locus_tag="ACZPFK_06200"
     CDS             2082..2225
                     /locus_tag="ACZPFK_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405936.1"
                     /translation="MLDYLNHLPKQLRPFLQKKEFCRGEPILRSGEENQKIYLVAQGD
                     AIG"
     gene            2226..2699
                     /locus_tag="ACZPFK_06205"
     CDS             2226..2699
                     /locus_tag="ACZPFK_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1405937.1"
                     /translation="MEGWNGSLIFVERYGPGDLFGELEVFCEGLRTTGITAQTACTVY
                     LLHKQYLLAWMEQDFSFTQALIRHLAEAMHRYADNVTQLQLMTVEERVKKCILTYEAM
                     GMLDKLDKKALMAGSMASLRSVNRAVSRLEEEGAVRVRKKKIAVLDRALLESGGA"
     gene            2884..>3845
                     /locus_tag="ACZPFK_06210"
     CDS             2884..>3845
                     /locus_tag="ACZPFK_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1405938.1"
                     /translation="MEKRICCEAVDRHAQELTELASKIWAEPEMGWNEVKASAWTAEY
                     LKEQGFEVELGAYGIPTAIRAVWGSGHPVIGFCGEYDCLPGLSQKVSARQDPVTPGGN
                     GQGCGHNLLGVGCVGACLGLKAELEAGGKEGTVVFYGCPAEEQLTGKGFMARGGAFQE
                     CDFTIAWHPASGNQNTLGNMTGVEGAFFRFKGRTSHAAMNPQDGRSALDAVQLMNMGV
                     EFLREHVTDDVRIHYNIVDGGLAPNIVPDTAGSKYFVRALSREAVLDAFERVVKCAEG
                     AAHMTDTTLEIERLGGIYPTLQNKVLAEVMQKAREEIPLPEYTA"
BASE COUNT          730 a         1127 c         1317 g          671 t
ORIGIN      
        1 ttgggggggg gggggggtgc ggcgggccgt ggcgcgtaca aacgcccctc agcgccggtc
       61 gagcagttcc cagaggtatt gcttgaagcc ggggccgtcc agctcggtga cgatcaggga
      121 gttgggctca tggtgctttt gtccaaacgc ctctttcata aaggaggagc ccggcgtctc
      181 gaaaatagtc gcgccgcggg tgtgctcgcc tttcaggtcc acgcgggtat agctctcaaa
      241 gctggtgcgg atcaaatcgg gacggaccag ggggatgaaa gcggcggggt cgggcaggtc
      301 gaagctgccc tcgccgtgta cgcagcgagc caggtcgatg gcggtgcaat tgcagtccac
      361 acagaagcgc ccagcctcat tcagctcgcg gaagcccttg atatccgcct cgtcgaaccg
      421 catgtccccc cagcaggcct ccagcgtcac catcgtgaaa ggcacgccga agctgcagac
      481 gatatcggcg gcctccgggt cgcacatgac gttgaattcc gcccagacgg tatggggatt
      541 gttgtagggc aggccgccgc ccatgatcgt gatgtggggg acctttttca tggtctcggg
      601 ctcctgcagt atcgccatgg ccaagttggt cagcgggccg atggcaaaga tctcgatgtc
      661 gccgtccccc tcccgcagaa gccgcagcat ggcgtccacc gcaaattccg gctccgcctc
      721 gcgcgtcggc tttttaaagg tgcccacatc ccccatgccg tccttgccgt gcacctggtc
      781 ggcgttttcg taggcgcagt aaaggggatg gctggcgccc ttgtagaccg gcgggcgata
      841 ggtatccgcg tattcgatcg tctgcaggca gttataggtg gcctggttca cctcgatgtt
      901 gccggataca gtggtgaaag cgacgacctg gacggagggc tcgcgcagcg ccatgagcag
      961 ggccacggcg tcgtcagaac cggtgtcggt gtcgataatg atctttttca tcctaaacct
     1021 ctctttctca gatgacgagc tggcacaaaa gctgcttgaa gccctcggcg tcgatgccgg
     1081 acaccagctg gcagttgtag ggcgggaaga cggagcgctt ggagacgaat tccaggcggt
     1141 cgcgggggcg caggtcattc accgtggcgc cgtaagcccg ctcgctcttc aactccacgc
     1201 gagtgtagct gggggcggcg tcccggacca gccccggccg cagcaggacc gccgccgcca
     1261 ccgcgtcggg gagggtgaag tagcgctctc cgtacgcggc gttggcctca taaagggtgc
     1321 ggttgcaatc gacgcagaaa tcggcgcagc gggagccggc cgcccgcagc cgggcacagt
     1381 ccgcttctgt gaagcgcgcc tcgccccggc aggcgtcgat cgtgaccatc gtaatgggca
     1441 cgccgaaggt aaagacgtaa tcggccgcct ccgcgtcctc ccacaggttg aattccgcca
     1501 ggtggccggt gttgcccttt ttataggcgc ctcccatgag ggtgatgtgg cccacccgcc
     1561 tcatcgtgtc cgggtcccgc atcatggcaa gggccaaatt ggtcaggggg ccgagggcga
     1621 ggatctccag gccgccctcc ccggcggcgc ccagcagggc ggtcaccgcc ttttccgacg
     1681 cgggctgccc gccgggctgc agggcgccca ggtcccccat gccgtcctcg ccgtgtacgg
     1741 ccacggcgga gcggtgttca tgaaagagcg ggcgctcgca gccgaggtag acggggggct
     1801 gggccgccgc agcataaccg atggtcatca gagcgttttt ggtggcctgc tccagcccta
     1861 cattgccggc cacagtggag atgcccacca cttccacgga gggatctctc agcatcatga
     1921 ccagggccac ggcgtcgtcc gaaccggtat cggtgtcgat ccaaaccttt tttgccattt
     1981 gaaattctcc tttcaggtcg tttcattgac accagcataa ccccggcact acaataaaaa
     2041 caggccaatt ggccaaaatc gaaaaaaatg agggatcgaa gatgctggac tacctaaacc
     2101 acctgccaaa acagctgcgt ccctttttgc agaaaaaaga attttgcagg ggggagccga
     2161 tcctgcgcag cggggaggaa aaccagaaga tttatctggt ggcacaaggc gatgccatcg
     2221 gttaggtgga gggctggaac gggtcgctga tctttgtgga gcggtacgga ccgggcgacc
     2281 tgttcgggga gttggaagtc ttttgtgagg ggctgcggac aaccggcatc accgcccaga
     2341 ccgcctgcac ggtctatctt ctgcacaaac aatacctgct ggcctggatg gagcaggact
     2401 tctcttttac ccaggcgctg atccgccacc tggcggaagc gatgcatcgc tatgcggaca
     2461 atgtcaccca gctgcagctg atgacggtgg aggaacgggt gaaaaaatgc atcctcacct
     2521 atgaggcgat ggggatgctg gacaagctgg acaaaaaggc cctgatggcg ggctccatgg
     2581 cctccctgcg cagcgtgaac cgggcggtga gccgcctgga ggaggagggg gccgtccggg
     2641 tcaggaaaaa gaagatcgcc gtcctggacc gggccttgct ggagagcggc ggggcgtgag
     2701 ggggagcgct tttccggccg gatggcgccc ggggacagga gagggccgga ggcagcgcga
     2761 gccggaccgg gcttgacgtt gccggacggc aggcagccgc agttgacaca cggacagagg
     2821 gggcttatac tgataacaat cgttttttat cacaggagca gggagtagga aaggggaagt
     2881 gaaatggaaa agaggatttg ttgtgaggcc gtggaccgcc acgcgcagga gctgaccgag
     2941 ctggcgtcca agatctgggc ggagccggag atgggctgga acgaggtaaa agccagcgcc
     3001 tggaccgccg aatatctcaa agaacagggg tttgaggtgg agctgggggc ctacggcatc
     3061 cccaccgcca tccgcgcggt ctggggcagc gggcatccgg tgatcggctt ttgcggcgag
     3121 tacgactgcc tgcccggcct gagccaaaag gtcagcgccc gtcaggaccc ggtgacgccg
     3181 ggcggcaacg ggcagggctg cgggcacaat ctgctgggcg tgggctgtgt gggcgcctgc
     3241 ctgggcctga aagctgagct ggaggccggc ggaaaagagg gcaccgtcgt cttttacggc
     3301 tgcccggccg aggagcagct caccggcaag ggctttatgg ccaggggcgg cgcgtttcag
     3361 gagtgcgatt tcaccatcgc ctggcatcct gcgtccggca accagaacac gctgggcaat
     3421 atgaccgggg tggagggcgc gttcttccgc ttcaagggcc gcacctctca tgccgccatg
     3481 aatccgcagg atggccgcag cgccctggac gcggtgcagc tgatgaacat gggggtggag
     3541 tttttgcgcg agcacgtcac cgacgacgtg cgcatccatt acaacatcgt ggacggcggc
     3601 cttgccccca acatcgtgcc ggacaccgcg ggcagcaagt attttgtgcg ggcactctcc
     3661 cgcgaggcgg tgctggacgc gtttgagcgg gtggtgaagt gcgccgaggg cgcggctcac
     3721 atgaccgaca ccaccctgga gatcgagcgg ctgggcggca tctatccgac cctgcagaac
     3781 aaggtgttgg ccgaggtgat gcagaaagcg cgggaggaga tccccctgcc cgagtacacc
     3841 gccga
//
LOCUS       DBMDYA010000207         3844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5518_length_3844_cov_1.185146, whole genome shotgun sequence.
ACCESSION   DBMDYA010000207 DBMDYA010000000
VERSION     DBMDYA010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5518_length_3844_cov_1.185146"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            471..1223
                     /locus_tag="ACZPFK_06215"
     CDS             471..1223
                     /locus_tag="ACZPFK_06215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1405939.1"
                     /translation="MEPEFVNLTAENLAREHLCCIIRSKKPHPGVEAKRQWLTERLKD
                     GHVFRKLNAKATVFIEYAPLETAWAPVLGDNYYYLYCLWVTGSCKGKGYGRALMEYCL
                     EDARKNGRSGVCMLGSQKQKAWLSDQSFAKKFGFETVDATENGYELLALSFDGTKPAF
                     TQNAKREKIEREELTVYYDAQCPYIYQSVGAVKEYCEANGIPASFILVDTLQKAKALP
                     CVFNNWAVFYKGRFETVNLLLDPAVLKRILKK"
     gene            1583..1771
                     /gene="rpmB"
                     /locus_tag="ACZPFK_06220"
     CDS             1583..1771
                     /gene="rpmB"
                     /locus_tag="ACZPFK_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR1405940.1"
                     /translation="MAKCDCCGKGMTFGIQVSHSHRRSNRTWKPNVKRVKAIVDGSPK
                     YIYACTRCLRSGKVTRAV"
     gene            complement(1849..2445)
                     /locus_tag="ACZPFK_06225"
     CDS             complement(1849..2445)
                     /locus_tag="ACZPFK_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405941.1"
                     /translation="MEDVLSAVENVDPDVVFLEARESVLEEYGVVDGPVDMAVVYSYC
                     TQNRIPAALVDWWVVDNEYRSNTTNEKRDDRIFANISEKLKKIRSGSKVLIVCGAGHF
                     YEQADRLLQHGARRLRLTDPGACFEADGGTFHYPESVEEVWEQRAYFYAYLYPKLVGA
                     DETLSEEIRADFTGGDHDAFYEEQLEYCELFAADLLYR"
     gene            complement(2923..>3844)
                     /locus_tag="ACZPFK_06230"
     CDS             complement(2923..>3844)
                     /locus_tag="ACZPFK_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1405942.1"
                     /translation="ITGGSTSSRLFQRLREELGLAYSVDSGTTAYLSGGLFEIQTAVN
                     PETAERTVEEILKTLEELKKGVSPTEFSRAREQLKAGANWIKVMATGGVMTKGVEPGS
                     PQLNEDELRAAIEEGHKAGVKSCTHSQGMTGIKNALRAGVDSIEHGFFMDDWCFDWMK
                     EHNVFYVPTLAAMYWIYVNGVEAGIADYVIRKVNSTFDAHRDTFLRAYKAGVKIALGT
                     DAGTPFNGHEKTAYEMVLMTQAGMSNWDALKTGTVNAAELCSVQDSLGSIEPGKRANF
                     AVFAGNPAEDIEAVMDCRMTVIGGEVKFQA"
BASE COUNT          797 a         1181 c         1005 g          861 t
ORIGIN      
        1 attgcgggac agatttctaa cagcgaatta catatatttg aggagtttgg tcacgctgca
       61 tacgaggaag caaaggactt taatcaacga atattgatgt tcttggataa ataatttttc
      121 ttatataact cccagtttga caggcggatt tactgcccct tattgacata atgtgattta
      181 aattggagaa cccccacacg gaaagagggt agaggttctc tttggtgatt cctccaaagc
      241 tggtttgtca gacctaatat caacacttct ctgacaggga gagccgcccc cacaaaatgc
      301 gcggccggaa aagcgcgtcg ggagtcggcc ccatcgaatc attttgagaa ccgcggactg
      361 atatggtatg ataaaggtga ccgccgcgcg ccgcttgctg cgcgcgggtc ccctccgcgc
      421 cggcacgccc tctttcaggg ccctggccgg gaacagaatc gaggtggaga atggaacctg
      481 aatttgtcaa cctgaccgcg gaaaaccttg cccgcgagca tttgtgctgc atcatccgca
      541 gcaaaaagcc ccacccgggg gtggaggcaa aacggcaatg gcttacagag cgactcaaag
      601 acggccacgt ctttcgcaag ctgaacgcga aagccacggt cttcatcgag tacgcccctc
      661 ttgaaacggc ctgggcgccc gtcctgggcg acaactacta ttatctgtat tgcctctggg
      721 tcaccggcag ctgcaagggc aagggttacg ggcgtgccct gatggagtat tgcctggaag
      781 acgcccgaaa aaacggcagg tcgggcgtct gcatgctggg ctcccaaaag caaaaggcct
      841 ggctctccga ccagtcgttt gcgaaaaaat tcggctttga gacggtcgac gccaccgaaa
      901 acggctacga gctgctcgcc ctctcttttg acggcacaaa gcccgctttc acacagaacg
      961 caaagcgtga aaagatcgag cgcgaagagc tgacggtcta ttatgacgcg cagtgcccct
     1021 atatctacca gagcgtcggg gcggtgaaag agtactgcga ggcaaacggc atccccgcct
     1081 cttttattct ggtggacacg ctgcaaaagg cgaaagcgct gccctgcgtt ttcaacaact
     1141 gggcggtatt ttataagggg cgctttgaga cggtgaacct gctgctggac cccgccgtcc
     1201 tgaagaggat cctcaaaaaa tgagacggcc cctccccccc cctgccacgg ggcggagaac
     1261 cgcaatcggg gccttggggc ccgcgcagga cgcggacgcg aaagacagtc tccgggcagg
     1321 gatgcagggg gctgctccgg aaaaagcctt tgcacttctt caccgcgccg gggacgcccc
     1381 tacttgtcgc cgggcgccgc agcgctcgcc cacggggcgc aagggcggcg gctttgccca
     1441 aaagcggccg gaccggggcg ggttatctgc cgttttttcc ctttcccctc ctcttttttg
     1501 cgttttttga gacaaacccc ttgcatttta gacttcggtt tgctattata ttatagtgac
     1561 ttgaaaagag gaggtgcaga agatggcaaa gtgtgattgc tgcggaaaag ggatgacttt
     1621 cggcatccag gtttcccatt cccatcggcg ttccaaccgt acctggaagc cgaacgtgaa
     1681 gagggtaaag gcgattgtag atggctcccc gaaatatatc tacgcctgca cccgttgcct
     1741 gcgttccggc aaggttaccc gggctgtctg agaatgacgt tcaaaagccg ctcaccgatg
     1801 gtgggcggct tttttcgtct tcctattctt aaggcagtgg tccgtacgct accggtacaa
     1861 cagatcggct gcaaacaact cgcagtactc cagctgttcc tcataaaacg cgtcgtgatc
     1921 tccccccgta aaatccgctc tgatctcctc gctcagcgtt tcatctgccc ccaccagctt
     1981 cgggtagaga taggcataaa aataggcgcg ctgttcccag acctcttcta cactttccgg
     2041 atagtggaac gtcccgccgt ctgcctcgaa acaggcgccc gggtctgtca gccgcagccg
     2101 ccgggctccg tgctgcaaca agcggtccgc ctgttcataa aaatgcccgg caccgcacac
     2161 gatcagcact ttcgaccccg accggatctt tttcagcttt tcgctgatgt tggcgaatat
     2221 cctgtcgtca cgcttctcat tcgttgtatt ggagcggtat tcattgtcca cgacccacca
     2281 atccaccagt gcggcaggaa tccgattttg agtacaatag ctgtatacga ccgccatgtc
     2341 caccggtcca tccaccacac cgtactcttc taatacggat tcccgcgcct cgagaaaaac
     2401 gacgtctggg tccacattct ccactgcgct caggacatct tccatcgaat agccgagcca
     2461 tcggtcaaaa tggttttcat gaaaggttcc cagcagataa acctgttgcc ctccctgcgt
     2521 ctcaaacccc ctgatgagac tgtttttgtt taagtatcgg ttccataaaa atgccgctac
     2581 ggccgcggca gcgagaagca agatccccac ggcgagcaga ctccgtcttt ttcttcttct
     2641 ttttccctct tgtcccgcca ttttgtcctc cccgtctatt cgctcccgct cttcgggcaa
     2701 aagcgacatc tttcgtcaaa gccgctccgt atgcaacgtc attttatcgt tgccggtcgg
     2761 tcctgtcaag caattttccc gtcccgacgc tccccacaaa aaattgcgga cggcaaagac
     2821 cttctctgcc gtccgcgatc tcatccttat ttcacgtatt gagtaatttg gtctgccgtt
     2881 ttctccacga atccgcatgc cggccgcagc ggcgcggcag tctcaggcct ggaacttcac
     2941 ctcgccgccg atcacggtca tgcggcagtc catgaccgcc tcgatgtcct cggcggggtt
     3001 gcccgcaaac accgcgaaat tcgcccgctt gcccggctcg atggacccca gagaatcctg
     3061 cacggagcag agctccgcgg cgttgacggt gccggtcttc agcgcgtccc agttggacat
     3121 gcccgcctgg gtcatcagca ccatctcata ggcggtcttt tcgtgcccgt tgaacggggt
     3181 gcccgcgtcg gtgcccagcg cgatcttgac cccggcctta taagcgcgca ggaaagtgtc
     3241 ccggtgcgcg tcgaaagtgc tgttcacctt gcggatcaca tagtcggcga tgcccgcctc
     3301 cacgccgttc acataaatcc agtacatggc cgccagggtg ggcacataga acacattgtg
     3361 ctctttcatc cagtcaaagc accagtcgtc cataaagaag ccgtgctcga tggaatccac
     3421 gcccgcccgc agcgcatttt tgatgccagt catgccctgg gaatgggtgc aggatttcac
     3481 gcccgccttg tgcccctctt cgatggcggc gcgcagctca tcctcgttca gctgcgggga
     3541 gcccggctcc acgcccttgg tcatcacgcc gccggtcgcc atcactttga tccagttggc
     3601 gcccgctttc agctgctctc tggcccggga aaattccgtg ggagacactc cctttttcag
     3661 ttcttccagt gtcttcagga tttcttccac agtccgctcc gcggtttccg gatttaccgc
     3721 cgtttgaatc tcaaacaggc cgccgcttag gtaagcggtg gtcccgctgt ccaccgaata
     3781 ggccagcccc agttcctccc gcagccgctg aaacagccgg gaagaagtgc tcccgcctgt
     3841 gatc
//
LOCUS       DBMDYA010000208         3837 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5528_length_3837_cov_1.457173, whole genome shotgun sequence.
ACCESSION   DBMDYA010000208 DBMDYA010000000
VERSION     DBMDYA010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_3837_cov_1.457173"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ACZPFK_06235"
     CDS             complement(<1..543)
                     /locus_tag="ACZPFK_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1405943.1"
                     /translation="MVMEEYGRWLKYATEDGDLIPELRSIEGKQDEIEDRFAVSLEFG
                     TAGLRGVIGAGTNRMNIYTVRQATHGLAVYLKKHNKHPKVAISYDSRNKSLEFARAAA
                     EVLAANGVKAYLYKTLMPTPMLSYAVRKLKCDAGIMVTASHNPAKYNGYKAYGPDGCQ
                     MTSESADAVYAEIQKTDVFKD"
     gene            843..2372
                     /locus_tag="ACZPFK_06240"
     CDS             843..2372
                     /locus_tag="ACZPFK_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1405944.1"
                     /translation="MYAKITSLGGLALEGFPVTVEADLSGGLPAFELVGLPDNAVKEA
                     RDRVRSALKNSGFTYPVSRITVNLAPADIKKTGPVYDLPVLLSILAASGQLPPPPPDT
                     AFLGELALDGAVRSVNGVLPMALAARAHGVKALFVPADNAPEAAVVEELQVYALHTVR
                     ELADHLRGGTPLLPTPPTRFSLSAGGPLPDFADVRGQPEARRALEIAAAGHHNLLLIG
                     PPGTGKSMLAQRLPSILPPMTREEAIETTEIYSVAGQLPRGSGLVTRRPFRSPHHSVS
                     SMGLAGGGSVPRPGEISLAHNGVLFLDELPEFRRDALELLRQPMEDGRVTVSRVAASA
                     TFPCGFMLVAAMNPCPCGYFGHPTRQCTCTPSAIDRYLQRISGPLLDRVDLHVEVPPV
                     DYGALSGEAQGEASSDILARVTAARARQQKRYEGAGISCNAQLTPALQRLHCPLTPAA
                     DRLLARAFQQMGLSARAYGRVLKVSRTIADLEGAQTIDAPHVSEAIQYRNLDRKYWHN
                     R"
     gene            complement(2758..3459)
                     /locus_tag="ACZPFK_06245"
     CDS             complement(2758..3459)
                     /locus_tag="ACZPFK_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405945.1"
                     /translation="MRERRQGRSPVANYLHNHALRLERQTAPWGTGLQLPVDEDGMIV
                     DQRRLAQYRMGVTDSAACGCGWIACYNALRLLGERPRPESVLKALERDLVLRGRMGTR
                     TVALPAFFLKRGYRVAVSFTLAGAEKRAPAADANIVYYLRGKHPSAHFVAFSGKVAEN
                     SEGEAVYRFYNSLTARLLPRRRPDGVETPCYCAGGHRGDRRTLAALLEPEAPLLKAVF
                     SISRPGGQAGRAGEN"
     gene            complement(3483..>3837)
                     /locus_tag="ACZPFK_06250"
     CDS             complement(3483..>3837)
                     /locus_tag="ACZPFK_06250"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0070038 - rRNA
                     (pseudouridine-N3-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1405946.1"
                     /translation="REKAAGEAEIRRALQKEGERILAAVPKNASLVALCIEGKQLGSE
                     ELAAFFQQKALSGRADVAFAIGSSHGLSDEVKARAALRLSMSRMTFPHQLARLMLLEQ
                     IYRAASINAGTRYHK"
BASE COUNT          567 a         1369 c         1233 g          668 t
ORIGIN      
        1 atccttgaac acgtcggtct tttgaatctc ggcgtagacc gcgtcggcgc tctcgctggt
       61 catctggcag ccgtcggggc cgtaggcctt gtagccgttg tattttgccg ggttgtggct
      121 ggccgtgacc atgatgccgg cgtcgcactt cagcttgcgc accgcgtaag agagcatcgg
      181 ggtgggcatc agcgtcttgt acagataggc cttgaccccg ttcgccgcca gcacctccgc
      241 cgcggcgcgg gcaaactcca ggctcttgtt gcggctgtcg taggagatgg ccaccttggg
      301 gtgcttgttg tgctttttca ggtagacggc caggccgtgg gtggcctggc ggacggtgta
      361 gatgttcatg cggttggtgc ccgcgccgat gacgccgcgc aggcccgccg tgccgaactc
      421 caggctgacc gcaaagcgat cctcgatctc gtcctgtttg ccctcgatcg agcgcagctc
      481 ggggatgagg tccccatcct ctgtggcgta cttcagccag cggccgtact cttccataac
      541 catttcatgc acctgcctga ttcaattttt tgcgggcggc cgcgcggggc gccgccgcgc
      601 cgcgggtggg ctttaccgtt cttttgcttg ctccctatta taatcctttc ctcaaaaaga
      661 gtaaagttgc agttgtatta agaaatcgat tcgcgcgcgg gggccgcgcc catttttttc
      721 aaaacagcgc ctttcccccc ctctccgccc cgtgggcggc aaaacagcgc ttgcaatcct
      781 ttcagggcgc gggtatcata aaaacagcga tgttgtcgga gcgcagagag ggggacgggc
      841 gcatgtacgc gaagatcacc agcctgggcg gactggcgct ggagggcttt cccgtgacgg
      901 tggaggccga tctgtccggc gggctgcccg ctttcgagct ggtgggcctg ccggacaatg
      961 cagtcaagga ggcgcgggac cgggtgcgca gcgccttgaa aaacagcggc ttcacctatc
     1021 cggtgagccg catcaccgta aacctggccc ccgccgacat caaaaagacc ggcccggtat
     1081 acgatctgcc ggtgctgctg tccatcctgg cggcgtccgg ccagctgccg ccacccccgc
     1141 cggacaccgc gtttctgggc gagttggctt tagacggcgc ggtgcgcagc gtcaacgggg
     1201 tgctgcccat ggcgctggcc gcccgcgctc acggcgtcaa agcgctgttc gtgcccgcgg
     1261 acaacgcgcc cgaggccgcg gtggtggagg agctgcaggt gtacgccctg cataccgtgc
     1321 gcgagctggc ggaccacctg cgcggcggca cgccgctttt gcccacgccg ccgacccgct
     1381 tttccctctc cgcgggcggc cccttacctg attttgcgga cgtgcgcggg cagccggagg
     1441 cgcgccgggc gctggagatc gccgcggcgg ggcaccacaa cctgctgctg atcggcccgc
     1501 cgggcaccgg caagtcgatg ctggcccagc gcctgccctc cattctgccc cccatgaccc
     1561 gggaggaggc catcgagacc accgagatct attcggtggc gggccagctg ccccggggca
     1621 gcggccttgt aacccgcagg ccctttcgca gcccgcacca ttcggtctct tccatggggc
     1681 tggccggcgg gggcagcgtg ccccgtcccg gcgagatcag cctcgcccac aacggggttc
     1741 tgtttttaga cgaattgccc gagtttcgcc gcgacgccct ggagctgctg cgccagccca
     1801 tggaggacgg ccgcgtcacg gtcagccggg tggcggccag cgccacgttc ccctgcgggt
     1861 ttatgctggt ggccgccatg aacccctgtc cctgcggcta tttcggccac cccacccgcc
     1921 agtgcacctg taccccctct gccatcgacc gctacctgca gcgcatctcc ggccccctgc
     1981 tggatcgggt cgacctgcac gtggaggtgc cgccggtgga ctacggagcg ctttccggcg
     2041 aggcgcaggg cgaggccagc agcgacattc tggcccgggt gaccgccgcc cgggcccgtc
     2101 agcaaaagcg gtatgagggc gccggcatca gctgcaacgc acagctcacg ccggctctgc
     2161 agcggcttca ctgcccgctc accccggcgg ccgaccggct gctggcccgc gcctttcaac
     2221 agatgggcct ctccgcccgc gcctacgggc gggtgctcaa ggtcagccgc accatcgccg
     2281 acctggaggg ggcgcaaacc atcgacgcgc cccatgtcag cgaggcgatt cagtaccgta
     2341 acctggaccg caaatactgg cacaaccgct gagatttatg ctccgcagcg tccggcggcc
     2401 gccgggcact gtcctcacgg agctgtgcag cgttgaaccc caaaaacaga tggaaaccgg
     2461 gtgctcgccg ccctcgcccg ccgcgccgca gtttgcgcaa ggtctgcgcc ggaggcgccc
     2521 gtttccatcc gcccgccccc tgtcgctgcg cagcaggcag cggcgggcgg agaaagtaca
     2581 aaacacggcg tcagccccaa caggctgacg ccgtgttttt gatgttggcc cgtggacagc
     2641 ttctgcagcc cggaggagag cgccgcccgg ctccgctttc ccctgtatgg agtcgccgcc
     2701 ctttccccgt cccacagagc gcagggcgcc gtgcggaacg cgcgccggcc gcgctgatca
     2761 gttctctccc gcgcgtcccg cctgcccgcc ggggcggctg atgctgaaca ccgctttcag
     2821 caacggcgct tccggctcga gcagcgccgc caaagtgcgg cggtcgcccc tgtggccgcc
     2881 cgcgcaatag cagggggtct ccacgccatc ggggcggcgg cgcggcagca gccgcgcggt
     2941 cagggagttg taaaagcgat acaccgcctc gccctcgctg ttttccgcca ccttgccgct
     3001 gaacgccacg aaatgggcgc tggggtgctt gccccgcaga tagtagacga tgttggcgtc
     3061 cgccgcgggg gcccgcttct ccgcccccgc cagcgtgaag ctgaccgcga cgcggtaacc
     3121 gcgttttaaa aagaacgcgg gcagggccac ggtgcgggtg cccatccgcc cgcgcagcac
     3181 caggtcgcgc tccagcgcct tgagcacgct ttcagggcgc ggccgctccc ccagcagccg
     3241 cagcgcgttg taacaggcga tccaaccgca gccgcaggcc gcggaatcgg tgacccccat
     3301 cctgtactgt gccagccgcc gctggtccac gatcatcccg tcctcgtcca ccggcagctg
     3361 cagccccgtc ccccaggggg cggtctgccg ctccagccgc agggcgtggt tgtgcagata
     3421 gtttgccacc gggcttctgc cctgccgcct ctcccgcatg gcgctcccct cctttgcccc
     3481 gctcatttgt gataccgcgt acccgcgttg atagaggccg cacggtaaat ctgctccagc
     3541 agcatcagcc gcgccagctg atgcggaaag gtcatccggc tcatggacag ccgcagcgca
     3601 gcgcgcgcct tgacctcgtc ggacaggccg tgggaggaac cgatggcaaa ggccacgtcg
     3661 gcgcggccgg agagggcttt ctgctgaaaa aaggccgcca gctcctcgct gcccagctgc
     3721 ttgccctcga tgcagagggc caccagcgag gcgttttttg gcaccgccgc caaaatgcgc
     3781 tctccctctt tttgcagcgc ccggcggatc tccgcctcgc ccgccgcttt ctcccgg
//
LOCUS       DBMDYA010000209         3826 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5550_length_3826_cov_1.588993, whole genome shotgun sequence.
ACCESSION   DBMDYA010000209 DBMDYA010000000
VERSION     DBMDYA010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_3826_cov_1.588993"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="ACZPFK_06255"
     CDS             complement(<1..410)
                     /locus_tag="ACZPFK_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405947.1"
                     /translation="MKKILLAAAALLLALLCAACSGPMGGSVEELLEAPKLSQSQTLV
                     MEGLNRYIGGSAKLQYPRRGEFLSPFVFKDINGDGVDEAVVFYGDEGSESKNKYVRVA
                     LLAQEDGKWAVLWDEEGLGTEVEAVSFSSMYGPDT"
     gene            complement(590..1372)
                     /locus_tag="ACZPFK_06260"
     CDS             complement(590..1372)
                     /locus_tag="ACZPFK_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014582.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1405948.1"
                     /translation="MLTAILMTTVAGLSTGLGGLIVLFCKRPTDQMMAFSLGFAGGVM
                     LTVSLSDMLPHAVEDYRAVMTNFRAVSAAISLFLLGMLIAWLLQHCLPEPKTERLAKP
                     GDGRDARQLALAAKSAIITTAVIVMHNLPEGVLTLFTGYANPAFGLTLTLAIALHNIP
                     EGIAVSVPVYYSTGSKGKGLAASLLSGLAEPAGALLAFFFFKDAITPLFLNGLVATIA
                     GIMSFVSVSELLPGGFSYGKPGWAVLGVSLGLFVMSLGVYLL"
     gene            complement(2487..3155)
                     /gene="deoC"
                     /locus_tag="ACZPFK_06265"
     CDS             complement(2487..3155)
                     /gene="deoC"
                     /locus_tag="ACZPFK_06265"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1405949.1"
                     /translation="MTREEVLARVDHTLLKPEARWQDIRTLCDEALKNHTASVCINSC
                     YVRRAAQYLQGRLPVCTVIGFPLGAMSTAAKVFEARQALADGASEFDMVINIGALKAG
                     EVDAVREEIRAVKAAVGDKVLKVIVETCLLTDAEKALMCDVVCEAGADFIKTSTGFST
                     GGATFEDIALFARCVKGRCRIKAAGGIRTVEDMERFLALGADRLGTSSAIKIFDAQNT
                     GGDY"
     gene            3286..3367
                     /locus_tag="ACZPFK_06270"
     tRNA            3286..3367
                     /locus_tag="ACZPFK_06270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3319..3321,aa:Leu,seq:tag)
     gene            complement(3618..>3826)
                     /locus_tag="ACZPFK_06275"
     CDS             complement(3618..>3826)
                     /locus_tag="ACZPFK_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405950.1"
                     /translation="LLQYIKKYRDICHGEDEMSVLKDFMLHLESPETRKLYGGEEENI
                     KMMQEFCLHIASKYFDAIVRDISK"
BASE COUNT          796 a         1245 c         1037 g          748 t
ORIGIN      
        1 gtgtccggcc cgtacatcga gctgaagctc accgcctcca cctcggtgcc caggccctcc
       61 tcgtcccaca gcacggccca cttgccgtcc tcctgggcca gcagggccac ccgcacatat
      121 ttgtttttcg actcgctgcc ctcgtccccg taaaagacga cggcctcgtc caccccgtcg
      181 ccattgatgt ccttgaagac gaaaggggag agaaactcgc cccggcgcgg atactgcaat
      241 tttgcactgc cgccgatata gcggttcagc ccctccatga ccagggtctg gctctggctg
      301 agcttgggcg cctccagcaa ttcttccaca ctgccgccca tggggccgga gcaagcggcg
      361 cacagcagcg ccagcagcag cgccgctgcc gccagaagga tttttttcat cccagcgccc
      421 ctttccggcc cggcccggac gcggcgcacg ctccgcccct ccgtcccatc cgatcccaaa
      481 cttcccgcgc cgggccgcaa aggcccggcc ctgatagatt attttaccac atgcgcgttc
      541 ttgaaacaag aagacatccc cgcgcacctg cccaggacct ccgccggttt cagagcagat
      601 agacccccag gctcatcacg aacagcccca gggacacccc cagcaccgcc catcccggct
      661 tgccgtaaga aaagccgccc ggcagcagct cgctgaccga gacaaagctc atgatgcccg
      721 cgatggtggc aaccagcccg ttgagaaaca gcggcgtgat cgcgtccttg aagaaaaaga
      781 aagccagcag cgccccggcg ggctccgcca ggccggacag cagggatgcc gccagaccct
      841 tgcccttgga gccggtagaa tagtagaccg gcaccgagac ggcgatgccc tcggggatat
      901 tgtgcagcgc gatggcgagg gtcagcgtca ggccaaaagc ggggtttgca tagccggtaa
      961 acagggtgag cacaccctcg ggcaggttat gcatcacaat gacggcagtg gtgatgatgg
     1021 cgctcttggc cgccagggcc agctggcgcg catcacggcc atcccccggc tttgccaggc
     1081 gttcggtctt gggctccggc aggcaatgct gcagcagcca ggcgatgagc atgcccagca
     1141 gaaaaaggct gatcgccgcg ctcacggcgc gaaaattggt catcacggcg cggtaatcct
     1201 ccaccgcgtg gggcagcata tccgagagcg aaacagtgag catcacaccc ccggcaaacc
     1261 ccagagaaaa agccatcatc tggtcggtgg ggcgcttgca gaacagcaca atcaggcccc
     1321 caaggcccgt ggaaaggccc gccaccgtcg tcatcaatat cgcagtcagc atacacaatc
     1381 ccccaaagtt taacatgcag ttttgtacag tatattaact ttttgcgaaa tcatgctgcc
     1441 gggcggaccg gccggggagc tgttcttcgc ccgcgggaag cgcggcatca aggcggcggg
     1501 aatgagggcg ggcgacggcg cggcgaaaga atgcgcggcg aagagagcga cgcggagacg
     1561 agagcgatgc agtggagagt gcgatgcgac ggcgcgggct gcccaacggc gagggcgaca
     1621 cgtcggggga acgacaggga caagccccgg ccctgttaaa aatgtgcggt ctgggttctt
     1681 tccacgctcc cccctttctt ctcttgtgtc ccattccctg gcaaaaatgc ccgggccgtg
     1741 cgggaaatcg ttttttctct tcgcactttt gcggtgtgct cccctctccc cgcccgtttt
     1801 ttgtctgttg agcgccttta agagcgcaaa aaagcactcg cctcgacgcg ttgggataaa
     1861 aaagcattaa ttttataccg ataattctag ggttcagcat atttaaaaag gttggcggtc
     1921 cacagagatc gccaaccttt ttgtgcggag cttctatcaa aaccgccggt ccaaaactgc
     1981 cgcgcgtccc tgagaggaaa agccgcgatg caaaacttgg gaaatccgca caggtacttt
     2041 ctgcggtcgc gggatgactt tgacgacgct ccgcgcgcaa acctctcccg gaagcactgt
     2101 tttcttgccc ccctgcatcg gcttttatcg cttcatctcc atataaaata aatgcattcg
     2161 gcccgtgcgc tcgctgttca ccgaacccac cttttatcgc cctgagctgc cttgcgaccc
     2221 tttctgcctc atcgtcttgc ccggcagggc tatcagagct gctttatgga acattccggc
     2281 ccggactcaa aaaacccggc cgcgcgggaa gacagggccc caaatatata aagagcagac
     2341 acagaaccaa aattctttca aaaacttatt acaaaacgag ccgaaagcgt tccgctgccg
     2401 tgctgtgccc agtcatcggc aaagcagcgg cagtccgcgc tgcttttgcc cctagggacg
     2461 ccgcccaaaa cgacggacag aggggttcaa tagtccccgc ccgtgttctg tgcgtcaaaa
     2521 attttgatcg cgctgctggt gcccaaacga tccgccccca gcgcaaggaa gcgctccata
     2581 tcctccaccg tgcggatgcc gcccgccgct ttgatgcggc agcgcccttt gacacagcgc
     2641 gcaaacagcg caatatcctc aaaggtcgcg cccccggtgg aaaagccggt gctggtcttg
     2701 ataaagtccg cgcccgcctc gcacaccacg tcgcacatca gcgccttttc cgcatcggtc
     2761 agcaggcagg tctcgacgat gactttcagc accttgtcgc ccaccgccgc cttgacggcg
     2821 cggatctcct cccgcacggc gtccacttcc ccggccttga gggcgccgat gttgatcacc
     2881 atgtcaaact ccgacgcgcc gtccgccagc gcctgccggg cctcaaacac ctttgccgcg
     2941 gtgctcatcg cccccagcgg gaagccgatc accgtgcaca cgggcagccg cccctgtaga
     3001 tactgggccg cgcgccgcac atagcaggag ttgatgcaga ccgacgcggt gtggtttttc
     3061 agcgcctcgt cgcaaagcgt gcggatatcc tgccagcgcg cctcgggctt cagcagcgtg
     3121 tgatccaccc ttgcaaggac ttcttccctg gtcataagtg ccgtcccctt tcctgatcgc
     3181 cgcacaagcc gcggcgtctc tgtcctcatt gtaatgccgc gcccctccgc gcgtcaagcg
     3241 gcccgttgac aagcgggggg caaactgata aactgaaaaa catctgcggg agtggcggaa
     3301 cggcagacgc gccggtctta ggagccggta cctcggtgtg tgggttcaaa tcccatctcc
     3361 cgcaccagtt taaaagcacc cctttcggca caggtccccg cctttggcga ggctttttcg
     3421 ccggacgggg tgcttcttcg cttttggggc gaatcccttt cactgggctt cgccctgaga
     3481 atgccgcctg cgtgcggcag gacgtgggac acgacgatcg attctgcacg gcagaggcag
     3541 cgcgaacgac cctcaaatgg tcataagatc aaaaagcaga tcagcttttt tgacctgctt
     3601 tttgctctta ccaatcgcta cttcgaaata tctctgacga tcgcatcaaa atattttgat
     3661 gcgatgtgca ggcaaaactc ctgcatcatt tttatatttt cttcctctcc cccataaagc
     3721 tttctcgttt caggcgattc taagtggagc ataaaatctt ttaatacgga catctcatcc
     3781 tcaccgtgac aaatgtcgcg gtattttttt atatattgca gcaata
//
LOCUS       DBMDYA010000210         3824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5552_length_3824_cov_1.214343, whole genome shotgun sequence.
ACCESSION   DBMDYA010000210 DBMDYA010000000
VERSION     DBMDYA010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5552_length_3824_cov_1.214343"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    337..346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<347..653)
                     /locus_tag="ACZPFK_06280"
     CDS             complement(<347..653)
                     /locus_tag="ACZPFK_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HIR1405951.1"
                     /translation="MLFTSENEIDLRERSPLALAFVGDGVYELLVRAREVERTRLKPN
                     RLHSGAVRFVSARGQFEALRYLEPMLTEAEKDLVRRGHNASKATVAKNASPEEYRAST
                     "
     gene            complement(686..>1320)
                     /gene="cgtA"
                     /locus_tag="ACZPFK_06285"
     CDS             complement(686..>1320)
                     /gene="cgtA"
                     /locus_tag="ACZPFK_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR1405952.1"
                     /translation="IPGLIEGAAEGVGLGHDFLRHVERCRLLLHVVDVSGCEGRDPAD
                     DFEKINGELERFSPALAQRPQIVLGNKCDIAAEEQVAAFRAFVERKGLVFLPVSAATG
                     KGLEALPGLVYNCLRELPPVEVFTPDYVKPAARSGREFTARREEDGSFYVDAPWLERI
                     LEGSNVEDYESLQYFQTQLADFGVLDKLVELGVKEGDTVRIGEYEFDYLF"
     assembly_gap    1321..1330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1331..1964)
                     /gene="cgtA"
                     /locus_tag="ACZPFK_06290"
     CDS             complement(<1331..1964)
                     /gene="cgtA"
                     /locus_tag="ACZPFK_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR1405953.1"
                     /translation="MFVDVARITVKAGRGGDGAVSFRREKYVAAGGPDGGDGGRGGDV
                     IFVADPNLSTLMDFRYKRKYAAQSGENGKSSNSYGKSGEDLVIRVPRGTLVKDAETGG
                     LIADISDDEPVVVAKGGRGGLGNRHFATPTRQIPRFAKPGLPGEEFTLQLELKLIADV
                     GLIGFPNVGKSTLLSRVSAAKPKIANYHFTTLSPVLGVVRVGEDASFVAAD"
     gene            complement(2122..2373)
                     /gene="rpmA"
                     /locus_tag="ACZPFK_06295"
     CDS             complement(2122..2373)
                     /gene="rpmA"
                     /locus_tag="ACZPFK_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1405954.1"
                     /translation="MAHKKGVGSTKNGRDSESKRLGVKRADGQFVLAGNILVRQRGTH
                     IHPGENVGQGSDDTLFALTDGVVRFERLGRDRKKVSVKQ"
     gene            complement(2392..2703)
                     /gene="rplU"
                     /locus_tag="ACZPFK_06300"
     CDS             complement(2392..2703)
                     /gene="rplU"
                     /locus_tag="ACZPFK_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1405955.1"
                     /translation="MYAVIVTGGKQYRVQKGDVVYVEKLPAEAGAEVVFDQVLAVGEE
                     GAVKFGAPVVEGASVKGTVEKQGKGKKIHVLRYRPKKGSMRKIGHRQPFTKVTITEIV
                     G"
     gene            complement(2916..3767)
                     /locus_tag="ACZPFK_06305"
     CDS             complement(2916..3767)
                     /locus_tag="ACZPFK_06305"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR1405956.1"
                     /translation="MERIDGKALAAKVKAEVAAEVEALRAQGVAPKLTVILVGEDPAS
                     QVYVGGKAKDCAQCGIDSEVLRLPDSTTEEQLLAEIAARNDDPTVSGLLVQLPLPRHI
                     HEKRVIEAISPEKDVDGFTPVNVGRLVIGEDCFLPCTPAGCIELLKATGEPLEGKRAV
                     VLGRSNIVGKPMALLLLRENATVTVCHSRTRDLPAVVRQADILVAAIGKKRFVTPDMV
                     KEGALVIDVGINRGEDGKLYGDVDEPGLAGKAGRITPVPGGVGLMTRAMLMVNTLKAA
                     KRQRGLG"
BASE COUNT          648 a         1351 c         1096 g          709 t
ORIGIN      
        1 ctcgaaccgc tccagcaaca attcgagcaa ccgttccagc aacaattcca gctcgcgcaa
       61 cagctccagc aatcgttcga acaacagctc cagcaacagc cgcaatgcga aaaactgccg
      121 ctaaaagcgg gcaggtgccg ctgatccagg cggcgcatcc gactgttttt caaacagccg
      181 ttttgaaacc ggactgccgg gttcaaagcg gctgtttgcc ttttttactc agcccacggc
      241 ccactgggcg gaaaaatgat cccacaccgc gtcgaacagc tcttggatgc gatcgttttt
      301 gccctccagg taaagatacc ccagcaggca ctcaaannnn nnnnnnccgt agaggcgcga
      361 tactcctccg gcgaggcgtt tttggccacc gtggccttgc tggcattgtg gccgcggcgc
      421 accagatcct tttccgcctc ggtgagcatg ggttccagat accgcagcgc ctcaaactgg
      481 cccctggcgc tgacaaagcg cacggcgccg ctgtgcaggc ggttgggctt caggcgggtg
      541 cgctccacct cgcgcgcgcg caccagcagc tcatagacgc cgtcccccac aaaagcgagg
      601 gcaagcgggc tgcgctcgcg cagatcgatc tcattttccg atgtgaacag caagcgccgt
      661 ccccctttcc cccatgacag ggttgtcaga acagatagtc aaattcatat tcaccgatgc
      721 ggacggtgtc gccctccttg acccccagtt ccaccagctt gtccagcacc ccaaaatcgg
      781 ccagctgggt ctgaaagtac tgcaggctct cgtagtcctc cacgttgctg ccctcgagga
      841 tgcgctccag ccagggcgcg tccacataaa agctgccgtc ctcttccctg cgggcggtga
      901 actcgcgccc actgcgggcc gcaggcttga catagtccgg cgtgaagacc tctacgggag
      961 gcagctcccg caggcagttg tacaccaggc ccggcagcgc ctccaggccc ttgccggtgg
     1021 cggcgctcac cggcagaaag accagccctt tccgctccac aaaggcccgg aaagcggcga
     1081 cctgctcctc agcggcgata tcgcacttgt tgcccaacac gatctgcggg cgctgcgcca
     1141 gggccgggga aaagcgctcc agctccccgt tgatcttctc aaaatcgtcg gcggggtcgc
     1201 gcccctcgca gcccgagaca tccaccacgt gcagcaacag gcggcagcgc tccacatggc
     1261 gcaaaaagtc gtgccccagg ccgacgccct ccgccgcgcc ctcgatcagg ccggggatat
     1321 nnnnnnnnnn tatccgccgc cacgaagctg gcatcctccc ccacccgcac gacgcccagc
     1381 accggcgaca gggtggtgaa gtgataattc gcgatcttcg gctttgcggc gctgacgcgc
     1441 gagagcagcg tgcttttgcc cacgttggga aagccgatca gccccacgtc cgcgatcagc
     1501 ttcagctcca gctgcagggt aaactcctcc ccgggcagcc ccggcttggc aaagcgcggg
     1561 atctgccggg tgggggtggc gaaatgccgg ttgcccaggc cgccccggcc ccccttggcc
     1621 accaccaccg gctcatcgtc cgagatatcg gcgatcaggc cgccggtctc ggcgtctttc
     1681 accagggtgc cgcggggcac ccggatcacc aggtcctccc cggacttgcc gtagctgttg
     1741 ctggacttgc cgttctcgcc gctctgggcg gcatacttcc gcttgtagcg aaagtccatc
     1801 agggtggaca aatttgggtc ggcgacgaag atcacgtccc cgccccggcc gccgtcgccg
     1861 ccgtccggcc cgccggccgc cacatatttt tcccggcgga acgagaccgc cccgtcgccg
     1921 cccctgccgg ccttgaccgt gatccttgcc acatccacaa acatgggctt tcacctccgt
     1981 tttttatcgt cgcgccgtgg gccgccgggc ccacaaaact agcttgcccg gccggcggcg
     2041 tcgcatacgc ggccgccccg cggacagaaa aagggggtaa aacgctgccg cgttttgccc
     2101 cctctgcatt tcagatgctg attactgctt gacgctgacc tttttgcggt cccggccgag
     2161 acgctcaaaa cgaaccacgc cgtcggtgag ggcaaacagg gtatcgtcgc tgccctggcc
     2221 gacattctca cccggatgga tgtgagtgcc gcgctggcgg acgaggatgt tgccagcgag
     2281 aacgaactgg ccgtcggcgc gcttgacgcc gagacgcttg gactcggaat cgcggccgtt
     2341 tttggtggag cctacgcctt ttttatgtgc catgctgttt acacctcccg attaaccgac
     2401 aatctcagtg atagtgactt tggtaaaggg ctggcgatgg ccgatcttgc gcatgctgcc
     2461 ctttttggga cgatagcgca ggacgtggat ctttttgccc ttgccctgtt tctcgaccgt
     2521 gcccttgacg ctggcgccct cgacgaccgg agcgccgaac ttgacggccc cctcctcgcc
     2581 cacggcgagg acctggtcga aaacgacctc tgcgccggcc tcggccggaa gcttctcgac
     2641 gtaaacgaca tcgccctttt ggacgcgata ctgcttaccg ccggtaacga taactgcgta
     2701 cattcggata acctcttttt ttaagtctcg ctgccccggg cgggtgacga caccacttga
     2761 aaagccctgc acatgcggct ttaatactat accatatccc cgccgccgct gtcaagcaaa
     2821 aacagcccgc gggcaaaacc cgagtcctta caaaagccct atgtaccggt cggcacctcc
     2881 gcacccacaa aaagccctcc gcgcgggccg cgccctcagc ccaggccgcg ctggcgcttt
     2941 gccgccttca gggtgttcac catcagcatc gcgcgggtca tcaggcccac gccccccggc
     3001 accggggtga tgcggcccgc cttgcccgca aggcccggtt cgtctacgtc gccgtacagc
     3061 ttgccgtcct cgccgcggtt gatgcccacg tcgatcacca gcgccccctc cttgaccatg
     3121 tcgggggtga cgaacctctt tttgccgatg gccgccacca ggatatcggc ctgccgcacc
     3181 accgcgggca ggtccctggt gcgggagtgg cagacggtga cggtggcgtt ctcgcgcagc
     3241 agcagcaggg ccatgggctt gcccacgatg ttgctgcggc ccagcactac cgcccgcttg
     3301 ccctccagcg gctcgccggt ggctttcagc agttcgatgc agcccgccgg ggtacagggc
     3361 aaaaagcagt cctccccgat caccagccgg cccacgttca ccggcgtgaa gccgtccacg
     3421 tccttttcgg ggctgatggc ctcgatgacc cgcttttcat ggatatgccg gggcagcggc
     3481 agctgcacca gcaggccgct gacggtgggg tcgtcgttgc gcgcggcgat ctccgccagc
     3541 agctgttcct cggtggtgga atccggcagg cgcagcacct cgctgtcgat gccgcactgg
     3601 gcgcagtcct tggccttgcc ccccacatac acctggctgg cggggtcctc gcccaccagg
     3661 atcacggtca gcttgggcgc cacgccctgc gcgcgcagcg cctccacctc tgccgcgacc
     3721 tctgccttga cctttgcggc caatgccttt ccgtcaattc tctccatgag gctcctcctg
     3781 ttcctgttgt gcctgcccgt tttcggtggg cggcgtgtcg ctct
//
LOCUS       DBMDYA010000211         3807 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5574_length_3807_cov_1.449002, whole genome shotgun sequence.
ACCESSION   DBMDYA010000211 DBMDYA010000000
VERSION     DBMDYA010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_3807_cov_1.449002"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /gene="gyrA"
                     /locus_tag="ACZPFK_06310"
     CDS             complement(<1..1839)
                     /gene="gyrA"
                     /locus_tag="ACZPFK_06310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1405957.1"
                     /translation="MIENSITEGTKLIVRDIKEEMETAFIDYSMSVITARALPDVRDG
                     LKPVHRRILYVMHERGNDPSHPYRKSADTVGAVLGAYHPHGDASVYDAMVRLAQDFSL
                     RYPLVDGQGNFGSVDGDPPAAYRYTEARMSKMAVDLMTDIEKETVDFVPNFDESKMEP
                     SVLPSRIPNLLVNGSTGIAVGMATNIPPHNLREVIDGVICLIDDPEAELPQLMEHIKG
                     PDFPTGGIIMGRSGIRAAYATGRGKITLRGRAEIEEKKNGRFQIIITEIPYMVNKARM
                     LEHIADLVKEKRIEGISDLNDESNREGMRVVVELKKDANPQVVLNQLYSFTQLQDTVG
                     VIMLALDGGVPKVMNLKTMLQRYIEFQDEVVRRRTQFDLRKAEDRAHILEGLKRATDI
                     VDEIIAAIRACKGGKAEAKAAIMEKFEFDDLQATAIVNFQLGQLAGLEILKIENELGE
                     LHAKIDNWREILADDAKVLAIVKEELLAMRQKYGDDRRTEIATVSGEVDIEDLIPVED
                     CVYLLTHYGYVKRQPTGVYHSQRRGGRGITGLTRRDEDFVEELFIGSTHDYILFVTDQ
                     GRVYRIKGYEIPEGSRTAKGVNIVNLLPLAPDEKVQTMLKVSAESDE"
     gene            complement(1996..>3807)
                     /gene="gyrB"
                     /locus_tag="ACZPFK_06315"
     CDS             complement(1996..>3807)
                     /gene="gyrB"
                     /locus_tag="ACZPFK_06315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR1405958.1"
                     /translation="RGLHHLVYEIVDNAIDEALAGYCNHIEVTIKKGNIIEVIDNGRG
                     IPVDIQPKLGIPAVTVVYTVLHAGGKFGGDNSGYKVAGGLHGVGGSVVNALSEWLTVR
                     VRRDGQEYEQTFRRGVAEAPLKVIGKTETTGTTVLFKPDPEMFEELVFNYDTLLTRLR
                     EEAFLNAGVRITIADERGDEPVRESMCYEGGIRSFVEHIHRRRQLEVLHPEVIYLKGQ
                     QDDIIAEVALQYNSSYNELILSFANNVNTPDGGTHEEGFKSAITRVFNDFGREKGYLK
                     DKDENLSGSDVREGLTAVISVKLTDAQFEGQTKAKLGNTEVRGLLSGMVYDKLKEFFE
                     ENPGVAKAVFEKATQAARAREAAKRARELVRRKSALETSRMPGKLADCREKDPSKTEI
                     YIVEGDSAGGSAKMGRDSNIQAILPLWGKMLNVEKARLDKVYGNEKMMPVVTALGCGI
                     GEDFDLSRLRYHKVFIMADADVDGSHICTLMLTFFFRFMRPLIEGGHVYIAQPPLFKL
                     AKGQQVRYAYNEAEMSALSAEMGQGVKVSRYKGLGEMNPEQLWETTMNPENRVIVQIS
                     IEDAERADETFTILMGDKVGPRRDFIEKNAQYATLDV"
BASE COUNT          618 a         1198 c         1179 g          812 t
ORIGIN      
        1 ctcgtcgctc tctgccgaga ctttcagcat ggtctgcacc ttttcatccg gcgccagggg
       61 cagcaggttg acgatgttca cgcccttggc ggtgcggctg ccctcgggga tctcataccc
      121 cttgatgcgg tagacccggc cctgatcggt gacaaagagg atataatcgt gggtggagcc
      181 gatgaacagc tcctccacaa aatcctcgtc ccgccgggtg aggccggtga tgccgcggcc
      241 gccgcgccgc tggctgtgat agacgccggt gggctggcgc ttgacgtagc cgtagtgggt
      301 gagcagatag acgcaatcct ccacagggat caggtcctcg atgtccacct cgccggagac
      361 ggtggcgatc tcggtgcggc ggtcgtcgcc gtacttctgg cgcatggcca gcagctcctc
      421 tttgacgatg gccagcacct tggcgtcgtc ggcgaggatc tcacgccaat tgtcgatctt
      481 ggcgtgcagc tcgcccagct cattttcgat cttcaaaatc tcaaggcccg ccaactggcc
      541 cagctgaaag ttgacgatgg cggttgcctg caggtcgtca aactcaaatt tttccatgat
      601 ggcggccttg gcctccgcct tgccgccctt acaggcgcgg atcgcggcga tgatctcgtc
      661 cacgatgtcg gtggcccgct tgaggccttc caggatatgg gcgcggtcct cggccttgcg
      721 caggtcaaac tgggttctgc ggcggacgac ctcgtcctga aactcgatat accgctgcag
      781 catggtcttc aagttcatga ccttgggcac gccgccgtcc agcgccagca tgatcacgcc
      841 cacggtgtcc tgcagctggg taaagctgta gagctggttg agcaccacct gggggttggc
      901 gtctttcttc agctccacca ccacccgcat gccctcgcgg ttggattcgt cgttcagatc
      961 gctgatgccc tcgatgcgct tctccttgac caggtcggcg atgtgctcca gcatacgggc
     1021 cttgttgacc atatagggga tctcggtgat gatgatctga aaacggccgt tctttttctc
     1081 ttcaatctcg gcgcggccgc gcagggtgat cttgccgcgg ccggtggcat aggcggcgcg
     1141 gatgccgctg cgccccatga tgatgccccc ggtggggaag tccggccctt tgatgtgctc
     1201 catcagctgg ggcagctcgg cctccgggtc gtcgatcagg cagatgacgc cgtcgatgac
     1261 ctcgcgcagg ttgtggggcg ggatgttggt cgccatgccc accgcgatac cggtggagcc
     1321 gttgaccagc aggttcggga tgcggctggg cagcaccgag ggctccattt tggactcgtc
     1381 aaagttgggg acgaaatcca ccgtctcctt ttcgatgtcg gtcatcaggt ccaccgccat
     1441 cttgctcatg cgggcctcgg tgtaacgata ggccgcaggg ggatcgccgt ccaccgagcc
     1501 aaagttgccc tggccgtcca ccagcgggta gcgcagcgaa aaatcctgcg ccagccgcac
     1561 catggcgtca tagaccgagg cgtctccatg ggggtgatag gcgcccagca cggcgcccac
     1621 cgtgtcggcg gatttgcggt aagggtggga ggggtcgttg ccccgctcgt gcatcacata
     1681 caggatgcgg cggtgcaccg gcttgaggcc gtcccgcacg tcgggcaggg cgcgggccgt
     1741 gatgaccgac atcgagtagt cgatgaaagc ggtctccatc tcctccttga tgtcacgcac
     1801 aatcagctta gtaccttcgg taatcgaatt ctctatcatg gctgccttcc tttttcaaaa
     1861 gataaaagag gggagaaaga aacgggcggg ccgcgttttc tgtttcagcg cggcgggagg
     1921 cttttcagcc ccggacgcgc cgcaagcggc aaagcccttg cttttctttc ttcttttttt
     1981 gccgttctgc gccttttaca cgtccagcgt ggcgtattgg gcgttcttct caatgaaatc
     2041 gcggcgcggg cccaccttgt cgcccatcag gatggtgaaa gtctcgtcgg cgcgctcggc
     2101 gtcctcgatg gagatctgca cgatcacccg gttttcgggg ttcatggtgg tctcccacag
     2161 ctgctccggg ttcatctcgc ccaggccctt gtagcggctg accttgaccc cctggcccat
     2221 ctcggccgag agcgcggaca tctccgcctc gttgtaggca tagcgcacct gctgcccctt
     2281 ggccagcttg aacagcggcg gctgagcgat atagacatgg ccgccctcga tcagcgggcg
     2341 catgaagcga aaaaagaaag tgagcatcag cgtgcagata tgcgagccgt ccacgtcggc
     2401 atccgccatg atgaagacct tgtggtagcg cagccgggac aaatcgaaat cctccccgat
     2461 gccgcagccc aaagccgtta ccaccggcat catcttctcg ttgccgtaga ccttgtcgag
     2521 ccgcgccttt tccacgttga gcatcttgcc ccacaggggc aggatggcct ggatgttgga
     2581 gtcgcgcccc atcttggcgc tgccgcctgc cgaatcgccc tccacgatgt agatttcggt
     2641 cttggagggg tctttttcac ggcagtcggc cagcttgccg ggcatacggc tggtctcgag
     2701 ggccgatttg cggcgcacca gctcgcgggc gcgcttggcc gcctcgcggg cacgggccgc
     2761 ctgggtagcc ttttcaaaaa ccgccttggc cacgcccggg ttctcctcaa aaaattcttt
     2821 cagcttgtcg tataccatgc cggacagcag cccgcgcacc tcggtgttgc ccagctttgc
     2881 cttggtctga ccctcaaact gggcgtctgt gagcttgacg ctgatgaccg cggtcaagcc
     2941 ctcgcgcacg tcgctgccgg agagattttc atccttgtcc ttgaggtagc ccttttcgcg
     3001 gccgaaatcg ttgaacacgc gggtgatggc gcttttgaag ccctcctcgt gggtgccgcc
     3061 gtcgggggtg ttcacattgt tggcaaacga gaggatcagc tcattgtagc tgctgttgta
     3121 ctgcagcgcc acctcggcga tgatgtcgtc ctgctggccc ttgagataga tgacctccgg
     3181 gtgcagcacc tccagctggc ggcggcggtg gatgtgctcg acaaagctgc ggataccgcc
     3241 ctcatagcac atgctctcgc gcaccggctc atcgccgcgc tcgtcggcga tggtgatgcg
     3301 caccccggcg ttgaggaacg cctcctcccg cagacgggtg agcaaggtat catagttgaa
     3361 gaccagctcc tcgaacatct ccgggtcggg cttgaagagc acggtggtgc cggtggtctc
     3421 ggttttgccg atcaccttga gaggcgcctc cgccacgccg cggcggaacg tctgctcgta
     3481 ctcctgcccg tcccggcgca cccgcacggt gagccactcg gacagcgcgt tgaccacgct
     3541 gccgccgacg ccgtgcaggc cgccggccac cttgtagccg gagttgtcgc cgccgaactt
     3601 gccgcccgcg tgcagcaccg tatagacgac ggtgaccgcc gggatgccca gcttgggctg
     3661 gatatccacg gggatgccgc ggccgttgtc gatcacctcg atgatgttgc cctttttgat
     3721 ggtcacctcg atgtggttgc agtaaccggc cagcgcctcg tcgatggcgt tgtccacgat
     3781 ctcatagacc agatggtgca ggccgcg
//
LOCUS       DBMDYA010000212         3771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5623_length_3771_cov_1.911172, whole genome shotgun sequence.
ACCESSION   DBMDYA010000212 DBMDYA010000000
VERSION     DBMDYA010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_3771_cov_1.911172"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="ACZPFK_06320"
     CDS             complement(<1..501)
                     /locus_tag="ACZPFK_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1405959.1"
                     /translation="MFQFPTLPTPRPLLFGAIHMPAFVYGTSQDLRKIETWLLRNVEN
                     YIEGGFDSVMLQDNTPRCPGLETKSLVYISALSYAVSSRFPDFPLGLIIESNDAQNAV
                     TIAAAAHFQYVRCKVFVGAMQKPGGMMDGNAAEAFHFRKMVDRDISLFADIFDRMGAP
                     LYDRDYP"
     gene            complement(546..1562)
                     /locus_tag="ACZPFK_06325"
     CDS             complement(546..1562)
                     /locus_tag="ACZPFK_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1405960.1"
                     /translation="MKATIKDVAQLSQVSATTVSMILNNKPIRATAETRERVLAAAKK
                     LGYQPNPVAVALVTKKSRTIGLILPNIGNYFFSELSKLIERELDKNQYSLLFGNSMGT
                     SEKDLNYLELFINKGVDGIIFVRSSKTSKNYNDRFLKIVESAPVPIVLADRVIPNPLT
                     GSVSVDHKRGGYLAAKHLIELGHRRIACITAESTLGNVVDRLDGYKSALAEAGISFDP
                     DLICSGNFSIATGLSLTPGLLDKNITAIFAFNDLIGIGAYQACHRCGLRVPKDVSIVS
                     YDDILLSQVLDRPLTTVHQPIELIAREAVNLLFQQIDSGEKYKADIVLQPQLVVREST
                     APPR"
     gene            complement(1653..2573)
                     /locus_tag="ACZPFK_06330"
     CDS             complement(1653..2573)
                     /locus_tag="ACZPFK_06330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1405961.1"
                     /translation="MKNRLLVVGHLNLDHVRTQELEEEEIPGGGALYTSFAAALYGDP
                     VDLLSTRCPDLPQSILDALARAGVNTDNVLPILGKQRRSSMAYDIDLVRTNFAHKSPL
                     WLQATVEQVPRHLPLPKCGYSCIYLAPLLAYNQLLYAQWAHEKGIPVAMDLSEYYALE
                     NTEAVKNCLGLIDIFMPSEVELDEIFPELAGDLPAIIEKVRGYGVSLLVIKRSTTGSL
                     ICCFNEERCYKVGIRPLSHVTDATGAGDSFNGAFLSCYLATKDTVLSARYASAVASCC
                     IQQISYLGLLHETREYLLRLAETVPVEIVR"
     gene            complement(2597..3703)
                     /locus_tag="ACZPFK_06335"
     CDS             complement(2597..3703)
                     /locus_tag="ACZPFK_06335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1405962.1"
                     /translation="MGNLHMMEEIKRQGHLKKTAEAVENALIQCGQDIKNHKIGRVII
                     TGSGDSYYIGLCARLFFEELCPTCSIEVLSSMEFSKYGLHKADGKTAVIAISISGNVL
                     RTIECVEQAQHNGAYVIGITNNRQSRLAQVAKCPVCLDLDTEPTWTCGTLTYTGSLYA
                     LARTALALSPLKSPVVQARLDQLNSVLTSLEDVIRESEPVCRAAARNMRIIGNQPPVL
                     VLGAGPNLGTAKYGAAKFAEICSTLAIGQECEEFCHGEFWVVERANLVFVIAPSGKGF
                     SRTVEVAGILRRFGCDLFILSDDASLCTLGKFSVLMPSCDEALSPLAYAIPFQLISYY
                     YSYEKGLNPDTRDHTDPFRKECSRLLTRGKNTVL"
BASE COUNT          969 a          900 c         1020 g          882 t
ORIGIN      
        1 tggatagtct cggtcgtata gaggcgcccc catacggtca aaaatatctg caaaaaggga
       61 gatgtcacga tcgaccatct ttcgaaaatg aaaagcctct gccgcgttac cgtccatcat
      121 cccccccggc ttttgcatgg cgccaacaaa aactttgcat cgcacgtatt ggaaatgggc
      181 ggcggcggcg atggtaacgg cattttgggc atcattgctc tcaatgataa gcccgagagg
      241 aaaatctgga aaacgagaag agactgcata gcttaaagct gagatataga ccagggattt
      301 cgtctccaag ccaggacaac gaggcgtgtt gtcttgcagc atgacgctgt caaaaccacc
      361 ttcgatataa ttttccacat tgcggagaag ccacgtctct atttttcgca gatcctgtga
      421 ggttccgtac acaaaagcgg gcatatggat ggccccaaaa agcaaggggc gcggcgtggg
      481 cagagtggga aattgaaaca ttgtgataca ctcctttgtc gggacccaaa gggagaactt
      541 ttgggctagc ggggaggagc agtgctttcc cgaacgacta gctgcggttg cagaacgata
      601 tctgccttat atttctctcc tgagtcaatt tgctgaaaga gcagattgac cgcttcgcgg
      661 gcaataagct caatgggctg atgtacagtg gtcaaaggcc gatccaacac ctgtgataga
      721 agaatgtcat cataagagac gatggagaca tccttcggaa cgcgtaggcc gcagcgatgg
      781 caagcctgat aggcaccgat gccgatcaag tcgttaaaag caaaaatagc ggtgatgttt
      841 ttgtcgagaa gaccgggcgt taaggaaagc ccggtggcaa tagaaaagtt gccggaacag
      901 ataagatcgg gatcaaagga aatgcccgct tcagccaggg cgcttttgta accgtctagc
      961 cgatcgacga cgttgccgag ggtggattct gcggtgatac aagcgatgcg ccggtgcccg
     1021 agctcaatga gatgctttgc ggccagatat cctccccgtt tgtgatccac actcacagaa
     1081 cctgtgagtg ggttgggaat gacccgatcc gccaaaacga tggggacagg cgcactctct
     1141 acaattttta aaaagcggtc attataattt ttgcttgtct tggaggaacg gacaaaaata
     1201 atgccgtcca cgcctttatt gatgaagagc tccagatagt tcagatcttt ttcagatgtg
     1261 cccatagagt tgccgaacaa cagggaatat tggtttttat ccagctcacg ctcgattagt
     1321 ttggagagct cggaaaaaaa atagtttcca atattgggca ggataagacc gatcgtgcgg
     1381 ctcttttttg tgaccagcgc tacagccacg ggattcggct gatacccgag cttttttgcg
     1441 gcggccaaaa cccgttctct cgtttcagcg gtggcgcgga tgggtttgtt gttcagtatc
     1501 atggaaaccg tcgtcgcgga gacctgggaa agctgtgcaa catctttgat cgtcgctttc
     1561 ataaaaaaat cgccccttcg tcaaacaatc atctcatagg caggttcctc tttataacta
     1621 taccttaatt tggtaaaaat gttaaatttt atttaacgca cgatttcaac aggtaccgtt
     1681 tctgcgaggc gcaacagata ctcgcgcgtc tcatgtagca gaccgagata actgatttgc
     1741 tgaatacagc agcttgccac tgcagaggca taacgggcgc tcaatacggt gtctttcgtg
     1801 gccaaatagc acgagagaaa agctccgtta aaactatctc ctgcaccggt tgcatccgtc
     1861 acatgagaca gggggcggat acccaccttg tagcagcgct cctcattgaa acagcagatt
     1921 aggctgcccg tagtagagcg cttgattacc aagagagaga ccccatagcc tcgcactttt
     1981 tcgatgatcg cgggcagatc accggcaagt tccggaaaga tctcgtctag ctcgacttcg
     2041 gacggcatga agatatcgat taggccaaga caatttttta ccgcttctgt attttccagg
     2101 gcgtaatact cggagagatc catggcaacg gggatccctt tttcgtgagc ccattgggcg
     2161 taaagcagct ggttgtaggc cagcagaggg gcgagataga tgcaactgta gccgcacttt
     2221 ggcagcggca gatggcgtgg gacctgctca acggtggcct gtagccaaag tggagattta
     2281 tgtgcaaaat tggtgcggac gagatcgata tcataagcca tggaagaacg gcgctgtttg
     2341 ccgaggatag gcaacacgtt gtcggtgttc actccggcgc gggccagcgc gtccagaatt
     2401 gattgcggca gatcaggaca acgggtggag agaagatcca ccggatcgcc ataaagggcg
     2461 gcggcgaacg aagtgtaaag tgctccgccg cccggaattt cttcttcttc cagctcctga
     2521 gtacggacat gatccaaatt taaatggccg acgaccaaaa gacgattttt catgagggta
     2581 tctgcctcct cccaaatcaa agtaccgtgt tttttccgcg ggtaagcagg cggctgcact
     2641 ctttgcggaa agggtcggta tgatcccgcg tgtcggggtt caaccctttt tcgtaagaat
     2701 agtaatatga tatcaactga aacgggatgg cgtaagcgag gggggataag gcctcgtcgc
     2761 aggagggcat gagaacagaa aactttccca gagtgcacag actggcatcg tcactgagaa
     2821 taaacaggtc gcagccaaat ctgcgtaaga taccggccac ttccaccgtg cgggaaaatc
     2881 ctttgccaga gggggcgatc acaaaaacaa gattggcgcg ctccacaacc caaaattcgc
     2941 cgtgacaaaa ttcttcacat tcctggccaa tcgccagcgt ggaacagatt tcggcaaatt
     3001 ttgcagcgcc gtacttcgct gtgcccaggt tggggccggc acctaataca agtactggcg
     3061 gctggtttcc gatgatacgc atattgcggg ccgcagcccg gcagacgggt tcgctttcgc
     3121 ggataacgtc ctccagtgag gtgaggacgc tgtttaactg atctaagcgg gcttgtacga
     3181 cagggctttt gagaggggaa agtgcaagcg ccgtgcgagc aagggcatat agactgcccg
     3241 tataggtcag ggttccgcag gtccaggtgg gctctgtgtc aaggtcaagg cagacagggc
     3301 atttggcgac ctgcgcgagc cggctttgcc ggttgttggt gatgccaatc acataagctc
     3361 cgttgtgctg ggcctgctcg acgcattcga tcgtgcgcag aacattgccg gagatagata
     3421 tggcgatgac cgctgttttg ccgtctgctt tatgaaggcc atacttagag aactccatgg
     3481 aacttaaaac ttcgatagaa caggtggggc aaagctcttc aaaaaaaaga cgcgcacaga
     3541 gcccgatata gtaggaatca ccgctgccgg tgatgatgac tctgccgatc ttgtggtttt
     3601 tgatgtcctg gccgcactgt atcagggcgt tttctacagc ttcagcagtc ttttttagat
     3661 gtccctgcct ttttatttcc tccatcatgt gcaaattgcc catatccgac ctcccattaa
     3721 aaaatgaaat aaataaaatg aaaaaatcgg ctgataatac gttttttcac a
//
LOCUS       DBMDYA010000213         3766 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5629_length_3766_cov_1.539154, whole genome shotgun sequence.
ACCESSION   DBMDYA010000213 DBMDYA010000000
VERSION     DBMDYA010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_3766_cov_1.539154"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="ACZPFK_06340"
     CDS             <1..989
                     /locus_tag="ACZPFK_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1405963.1"
                     /translation="IKNGYCTQFLVNKNDGASSNKLRAFLESNGDSVVVIEDDEVINC
                     HVHTTDPGKIVSYALNFGYLTNFKIENMHEQFLARQAQGKGLEKQAEAEKNPVKQKFQ
                     YSAVDPEMRFGFVAVAAGDGLRDIFTDIGVNAVVSGGQTMNPSTEDILEAVQSVPAKT
                     VFVLPNNKNIIMAAEQAVRLADRQIIVLPTRTIPQGITAMLNFDESLSGEDNAIAMMQ
                     AADRVATGLVTFAARNSDYEGHKIKQGEILALENGKLSFTDKDVAHAASKLTRSLLKK
                     DSSFVTVISGCDVPEEDAQRLTAQLRDKLPGSVEVTHLVGGQPVYYYIISVE"
     gene            complement(1789..2439)
                     /locus_tag="ACZPFK_06345"
     CDS             complement(1789..2439)
                     /locus_tag="ACZPFK_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1405964.1"
                     /translation="MQGRRFAQGLCWQKLFWVLVAGSVFGVAWEHCVNLLLHGRFYGG
                     RGLVWGPFNLVYGCGAVLLTLLLWPLRDGQAHKLLLAGMLAGSAYEVGCSLLQEAVFG
                     AVSWNYDRELLPLLGGRTTLPVAFAWGLLAVFYMKAVYPRLSRLIERIPPRWGRALTL
                     ALLIFLAVDALVSLLAVGRMAARRAALPAQGELDLLLDFCFPDHALRQIFPGMKFI"
     gene            2653..3321
                     /locus_tag="ACZPFK_06350"
     CDS             2653..3321
                     /locus_tag="ACZPFK_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019947.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1405965.1"
                     /translation="MRGEKGELPQALRGVAALLAGVLGPHAEVVVHDLQRSEILFIEN
                     GQLTGRAPGPTDDGDAIRLLAESADENGCLIGYLSSGKAARPLRSSNLFLRDQTGRLR
                     YTVCVNQDIGPFQQARRLLDELSGTAAPAPSAAPPQGEPGPSLRQLARSIILQEVERA
                     KPFSISTREGRLAVLARLEEKGVFDMRDAVPQVCELLQISQATLYNYQRELRGEQKKR
                     GAKT"
     gene            3318..>3766
                     /locus_tag="ACZPFK_06355"
     CDS             3318..>3766
                     /locus_tag="ACZPFK_06355"
                     /inference="COORDINATES: protein motif:HMM:NF028500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1405966.1"
                     /translation="MTLLSDSIQYLKGVGEKRAKQLGKLGLFTVGDLMRFYPRDYIDY
                     SLPYPLASAPYEAPCVVRATVWGKQAPVRVRGGRTLYRVNAGDDTAGLLITFFNSPYA
                     ADKLEPEKEYLFYGRVGGGLARREMVSPVFIAADSGTPLTAVYHLTEG"
BASE COUNT          738 a         1214 c         1157 g          657 t
ORIGIN      
        1 acatcaaaaa cggttactgc acccagtttc tggtgaacaa aaacgacggt gccagcagca
       61 ataagctgcg cgccttcctg gagagcaacg gcgacagcgt ggtggtgatc gaggacgacg
      121 aggtcatcaa ctgccacgtg cataccaccg acccgggcaa gatcgtcagc tatgcgctga
      181 acttcggcta cctgaccaac ttcaaaatcg aaaacatgca cgagcagttc ctggcccggc
      241 aggcccaggg caaggggctg gaaaagcagg ccgaggcgga gaaaaacccc gtcaagcaga
      301 agttccagta ctcggcggtg gaccccgaga tgcgcttcgg ctttgtggct gtggccgcgg
      361 gcgacggcct gcgggatatc ttcaccgaca tcggcgtcaa cgccgtggtc tcgggcgggc
      421 agaccatgaa ccccagcacc gaggacatcc tggaggcggt gcagagcgtg cccgccaaga
      481 ccgtcttcgt gctgcccaac aacaagaaca tcatcatggc cgccgagcag gcggtgcggc
      541 ttgcggaccg gcagatcatc gtgctgccca cccgcaccat cccccagggc atcaccgcca
      601 tgctcaactt cgacgagtcg ctctccggcg aggacaacgc catcgccatg atgcaggccg
      661 ccgaccgggt cgccaccggc cttgtcacct ttgcggcccg caacagcgac tacgagggac
      721 acaagatcaa gcagggcgag atcctggcac tggagaacgg caagctctcc tttaccgaca
      781 aggacgtggc ccacgcggcc tccaagttga cccgcagtct gctgaaaaag gactccagct
      841 ttgtcaccgt catctccggc tgcgacgtgc ccgaggagga tgcccagcgc ctgaccgccc
      901 agctgcgcga caagctgccc ggcagcgtgg aggtcaccca tctggtgggc ggccagccgg
      961 tttactacta tatcatcagc gtggaataag ccccgctttt cttctgcaaa gaggtcggcc
     1021 ttttgtgtgc cgccgtagat aagctcccct ggaaagagaa tcttcataga acgcggcgtc
     1081 aaatcggttg gataaccgca aagagttttg cggcttcgcc ccgcgctata tcggcggcgg
     1141 ttaaatcggc gctaccgcag gcgatgcgtc cgcacgtttt cagccccgca tcgcgcgctt
     1201 tcccttttta aaggcgcgca gcggcttcgg cgcggcggtc ttgaacgaac ggcaatataa
     1261 aaaatagccg taaggctgtt ctttcggcca tgtggcccat ccttgggcca cggccatcag
     1321 aatcagtcgt tttttattgc ggagcaataa aaaatagccg ctttgcggct atttttttcg
     1381 cgcatccgcc cactcccgtg ggcgggcgct caagatttta aggccgtggc ccgggacggc
     1441 ccacatggcc gtttttatag ctttgcgatg caaagctata aaaaatagcc gctttgcggc
     1501 tatttttttg gctatccgtt tcggttgccg cgcagcggcg agaaacgggg ccatagagat
     1561 aagttgtttt ttattgcgga gcaataaaaa aatagccgct ttgcggctat ttttttggcc
     1621 attcgtttcg gttgccgcgc agcgacgaga aacggggcca tcaagataag ttgtttttta
     1681 tgtgccggac gcggaaaacg tcgatccaaa cccagggcct cctcgccctt gcgcctttcg
     1741 gccgggcccc ttttttgccg ccgctctccc gcgcctttgc cgtgcccatc aaatgaattt
     1801 catgcccgga aagatctgcc gcagcgcgtg atcgggaaag cagaaatcca acagcaggtc
     1861 cagctcgccc tgggcgggca gggcggcccg ccgcgcggcc atgcggccca ccgccagcag
     1921 cgagaccagg gcgtccaccg ccaaaaagat cagcagcgcc agggtgaggg cccggcccca
     1981 gcgcggcggg atgcgctcga tcagccgcga gaggcggggg taaaccgctt tcatgtaaaa
     2041 gaccgccagc agcccccagg caaacgccac cggcagggtg gtgcgcccgc ccagcagcgg
     2101 cagcagctcg cggtcatagt tccagctcac cgcgccgaag accgcctcct gcagcaggct
     2161 gcagcccacc tcgtaggcgc tgcccgccag catcccggcc agcagcagct tgtgggcctg
     2221 cccgtcgcgc agcggccaga gcagcagggt gagcagcacc gcgccgcagc cgtacaccag
     2281 gttaaacggc ccccacacca ggccgcgccc gccgtaaaag cggccgtgca gcagcaggtt
     2341 gacgcagtgc tcccacgcca cgccgaacac gctgcccgcc accagcaccc agaacagctt
     2401 ctgccagcag agcccctggg caaatctccg gccttgcatc tcccgccacc ccctttattt
     2461 ataggacgaa tcaagcgaag ttttgtcgtc agcctttaca tttttatcgc tcgtgttata
     2521 atatttttac attaacctat aattttttta taatttgcgc tgtccctctg ctttatatcg
     2581 ccgtatccgc cccaagagac ggccggcggt gggggaaagg cgccggcaaa gggaaaaagg
     2641 ggaggcgtga ggatgcgcgg ggagaaaggt gaactgccgc aggcgctgcg gggcgtggcg
     2701 gcgctgctgg ccggggtact gggcccccat gccgaggtgg tggtccacga cctgcagcgg
     2761 tcggagatcc tcttcatcga aaacgggcag ctcaccgggc gcgcgcccgg ccccaccgac
     2821 gatggggacg ccatccgcct gttggcggag agcgcggacg aaaacggctg cctgatcggg
     2881 tatctctcct ccggcaaggc ggcccggccc ctgcgctcgt ccaatctttt tctgcgggat
     2941 cagacgggca ggctgcgcta taccgtctgc gtcaaccagg acatcggccc ctttcagcaa
     3001 gcgcgtcggc tgctggacga gctgtcgggc acggcggccc ccgcgccctc cgccgccccg
     3061 ccgcagggcg agccggggcc ctccctgcgc cagctggcgc gctccatcat cctgcaggag
     3121 gtggagcggg caaagccctt ttccatcagc acgcgggagg ggcggctcgc cgtgctggcc
     3181 cggctggagg aaaagggggt cttcgacatg cgggacgcgg tgccccaggt ctgcgagctg
     3241 ctgcagatct cccaggccac gctgtacaat tatcagaggg aactgcgcgg cgagcagaaa
     3301 aagagggggg ccaaaacgtg acgctgcttt cagacagcat ccaatacctg aaaggggtcg
     3361 gtgaaaagcg cgccaagcag ctgggaaagc tggggctctt caccgtcggc gacctgatgc
     3421 ggttttaccc ccgggactat atcgactact cgctgcccta cccgctggcc agcgccccct
     3481 atgaggcccc ctgcgtggtg cgggccaccg tttggggcaa gcaggccccg gtgcgggtgc
     3541 ggggcggccg caccctttac cgcgtgaacg cgggcgacga caccgccggg ctgctgatca
     3601 cctttttcaa cagcccctac gcggcggaca agctggagcc ggaaaaggag tatctctttt
     3661 acggccgggt gggcggcggc ctggcccgcc gggagatggt gagcccggtc ttcatcgccg
     3721 cggacagcgg cacgccgctc accgcggtct atcatttgac cgaggg
//
LOCUS       DBMDYA010000214         3750 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5651_length_3750_cov_1.567279, whole genome shotgun sequence.
ACCESSION   DBMDYA010000214 DBMDYA010000000
VERSION     DBMDYA010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5651_length_3750_cov_1.567279"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="ACZPFK_06360"
     CDS             <1..269
                     /locus_tag="ACZPFK_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405967.1"
                     /translation="RTKFVAAACGHAAAGTDGIALPNLTAAVSAKHTIHTPRRWFSRR
                     GPEGCGFEKQYNTKRRGGQPGWYFLRKKTEKFVQNGCNQKQLIL"
     gene            420..1769
                     /gene="gdhA"
                     /locus_tag="ACZPFK_06365"
     CDS             420..1769
                     /gene="gdhA"
                     /locus_tag="ACZPFK_06365"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR1405968.1"
                     /translation="MFKNAYLEKVYGEVAARSAGEPEFLQAVREVLESLETVVERRPE
                     LEKWGVLERLCEPERFVQFRVAWVDDAGRVQVNRGYRCQFNSAIGPYKGGLRLHPSVN
                     ASVIKFLGFEQTFKNSLTGLPMGGGKGGSDFDPKGKSDMEIMRFCQSFMTELYRHIGQ
                     FTDVPAGDIGVGAREIGYLFGQYKRITGEYSGVLTGKGLSYGGSLARTQATGYGLCYL
                     VQEMLVCMKGDSLGGKTAVISGSGNVAVYACEKAQRLGAKVVAMSDSNGYIYDRDGIR
                     LDVVKQIKEVRRGRIREYLDAVPGAEYHEGCRGIWTIPCDVALPCATQNELDEAAAKA
                     LIAGGVTAVGEGANMPCTLEATRALQAAGVLFAPAKAANAGGVATSGLEMSQNSLRLS
                     WTFEEVDARLKEIMIGIFHNAYDASRECGCEGNLVVGANVAGFLKVADAMLAQGVAY"
     gene            1876..2766
                     /locus_tag="ACZPFK_06370"
     CDS             1876..2766
                     /locus_tag="ACZPFK_06370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1405969.1"
                     /translation="MRLAFQVGEDGRGRRLSDFLRGRGLTAGLIKTVKYQPGGLRVDG
                     LPARTDLRLAAGQTVEVQLPPEPETAVPPQPVPLEIVYEDDFSAVLNKPAGVLVHPSA
                     PGCGGTLANGWRFLMEARGTPCPFRPVNRIDRNTSGLVLAAKNSFAAPLLARGFCKAY
                     WAVAEGELPLGAGAIDLPIRRAEGSIICRRAGGEGKPSRTEYAALAAAGGHTLLRAVP
                     VTGRTHQIRVHFSALGYPLAGDDLYGGGRSLIARQALHAGKIVFTPPLAEKEVVATAP
                     LPLDFLELLRRTGLPQPSLT"
     gene            3113..>3750
                     /locus_tag="ACZPFK_06375"
     CDS             3113..>3750
                     /locus_tag="ACZPFK_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405970.1"
                     /translation="MGVEAEYRAVLVGRGLRRAALFLGRCMARLLAPLAHKTARAARS
                     VKEDLSAPVRRARSGASHIRELVADEKKNGLWHALRLGLQYFWSGVRAYGHLAKNLVY
                     YLLPLGAGAVFVFTVVNVLDSQFALAVEYEGQVVGYVGQESVYEEAAQIVQNQLVYTG
                     HEQEWKLHPTFSIAAAKPDELSDSQTLVDAILRSSGEEITEAVGLYVNSRFYG"
BASE COUNT          697 a         1093 c         1351 g          609 t
ORIGIN      
        1 cgcggacaaa atttgtagcc gctgcctgcg ggcacgccgc agccggtaca gacggtattg
       61 ccctccccaa tctcacggcc gcagtttctg caaaacatac gatccacact ccccgtcgct
      121 ggttttcccg ccgcggccca gagggatgcg gctttgaaaa acagtataac acaaagcgtc
      181 ggggcgggca accgggatgg tattttttac gaaaaaaaac ggaaaaattt gtacagaatg
      241 gttgtaatca aaaacaattg attttataat aagtacattc aaaaccggct tgccgggcgg
      301 tgaaacggtg atgcgcagag gggcgcagcc gccgtcgcct gctgccggtg gagcgcgcgg
      361 ggcgtttccg ggaagggaag ctgaacgccc ggcgcggaat tttttaggag gggaaagcaa
      421 tgttcaaaaa tgcgtatctc gaaaaggtct atggcgaagt cgccgcccgc agcgccgggg
      481 aaccggagtt cttgcaggcg gtgcgcgagg tgctggagtc gctggaaaca gtggtggagc
      541 gccgcccgga gctggaaaaa tggggcgtcc tggagcggct ctgcgagccg gagcggtttg
      601 tgcagttccg ggtcgcctgg gtggacgacg cgggcagggt gcaggtgaac cggggctatc
      661 ggtgccagtt caactcggcc atcggcccct acaagggcgg cctgcggctg caccccagcg
      721 tgaacgcctc ggtcatcaag tttttgggct ttgagcagac ctttaaaaac agcctcaccg
      781 gcctgcccat gggcggcggc aagggcggca gcgactttga ccccaagggc aaatccgaca
      841 tggagatcat gcgcttttgc cagagcttta tgaccgagct ctaccgccat atcgggcagt
      901 tcaccgacgt gccggcgggg gatatcggtg tgggcgcgcg ggagatcggc tatctgttcg
      961 gccagtacaa gcggatcacc ggcgagtaca gcggggtgct gaccggcaag ggcctgtcct
     1021 acggcggcag cctggcgcgc acccaggcca ccggctacgg cctgtgctat ctggtgcagg
     1081 agatgctggt ctgcatgaaa ggcgacagcc tgggcggcaa gaccgccgtc atctccggca
     1141 gcggcaacgt ggccgtctac gcctgcgaaa aggcgcagag gctgggggcg aaagtagtgg
     1201 ccatgagcga ttccaacggt tatatttatg accgggacgg catccggctg gacgtggtca
     1261 agcagatcaa ggaggtgcgc cgcggccgca tccgcgaata cctggacgct gtgccgggcg
     1321 cggaatacca cgagggatgc cggggcatct ggaccatccc ctgcgatgtc gcgctgccct
     1381 gcgccaccca gaacgagctg gacgaggcgg ccgccaaggc actgatcgca ggcggtgtga
     1441 cggcggtggg cgagggcgcc aatatgccct gcacgctgga ggccacccgg gctttgcagg
     1501 cggcaggggt gctgttcgcg cccgccaagg cggccaacgc gggcggcgtc gccaccagcg
     1561 gcctggagat gagccagaac agcctgcgcc tctcctggac ctttgaggag gtggacgcgc
     1621 gcctgaaaga gatcatgatc ggcatcttcc acaacgctta cgacgccagc cgtgagtgcg
     1681 gctgcgaggg caacctggtg gtgggggcga acgtggccgg cttcctcaag gtggcggatg
     1741 cgatgctggc ccagggcgtg gcttactgac cgcctgggtg gcggcggcgt gaaaaatttg
     1801 gtataataaa atccgtgtgc cgggcggcgt cgccgcgggc gcacggattt ttgtctgtaa
     1861 aggggggcgg cgcggatgcg gctggccttt caggtggggg aggacgggcg ggggcggcgg
     1921 ctgtcggatt ttttgcgcgg ccgcggcctg acggccgggc tgatcaagac ggtaaaatac
     1981 cagcccgggg ggctgcgggt ggacggcctg cccgcccgca ccgacctgcg ccttgccgcc
     2041 ggacagaccg tggaggtaca gctgccgccg gagccggaga ccgcggtgcc gccgcagccg
     2101 gtgccgctgg aaatcgtgta tgaggatgat ttctccgcgg tgctgaacaa gcccgcgggc
     2161 gtcctggtcc acccctcggc cccgggctgt gggggaaccc ttgccaacgg ctggcgcttt
     2221 ttgatggagg cgcggggcac gccctgccct tttcgccccg tgaaccgcat cgaccgcaac
     2281 acctccggcc tggtgctggc ggcaaagaac agctttgccg cgccgctgct ggcccgcggc
     2341 ttttgcaagg cctactgggc ggtggccgag ggtgagcttc cccttggggc gggcgcgatc
     2401 gacctgccca tccggcgggc ggagggcagc atcatctgcc gccgcgcggg cggggagggc
     2461 aagcccagcc gcaccgagta cgcggcgctg gcggccgccg ggggacacac cctgctgcgg
     2521 gcggtgccgg tcaccgggcg cacccaccag atccgggtgc atttttccgc cctggggtat
     2581 ccgctggccg gggacgacct gtacgggggc ggccgcagcc tgatcgccag gcaggcgctc
     2641 catgccggga agatcgtctt caccccgccg ctggcagaaa aagaggtggt ggcgaccgcg
     2701 ccgctgccgc tggattttct cgagcttttg cggcggacag gcctgccgca gccctctttg
     2761 acctgagaaa agagagcgca aaaaattgcc aaaagcagga aaacatcgtg aaaaaagcgt
     2821 gaaaagggtt acaaaacgat tgcagaaccg gccccggcgc gtataatgtg aagtgtcggc
     2881 gcggccatcg ctgcgcgcaa aaggatggat gcgggccgcg tgaggcccgg gatagagagc
     2941 ggacgagcag accgcgcaaa gcgggatagg gaggtgcggc aagctggaga acacggaaaa
     3001 tacggcgcgc gcgccgcaga gcataagaaa gcggagaacg ggcagcccct ggtcgcaggc
     3061 caggcggcgg ctcttccgcc tgaggacatt tttaggagat ttattctacc gcgtgggagt
     3121 cgaggcggaa tatcgcgcgg tgctggtcgg acgcgggctg aggagggcgg cgctgtttct
     3181 cggccgctgc atggcccgtc tgctggcccc gctggcgcac aaaacggccc gggcggcccg
     3241 aagcgtgaaa gaggacctgt cggcccccgt gcgccgcgca agaagcggcg cgtcccatat
     3301 ccgcgagctg gtcgcggacg aaaaaaagaa cggcctctgg catgccctga ggctggggct
     3361 gcaatatttc tggtcgggtg tgcgggccta cggccatctg gcaaaaaacc tggtctacta
     3421 tctgctgccg ctgggggccg gggcggtctt tgtcttcacc gtggtcaacg tgctggacag
     3481 ccagtttgcc ctggcggtgg aatatgaggg ccaggtcgtg ggctatgtgg gccaggaatc
     3541 ggtctatgag gaggcggccc aaatcgtcca gaaccagctg gtctataccg gccacgagca
     3601 ggagtggaag ctgcatccca cgttcagcat cgccgcggcg aagccggacg agctgtccga
     3661 cagccagacg ctggtggacg cgatcctgcg cagctcgggc gaggagatca ccgaggcggt
     3721 ggggctgtat gtgaacagcc gcttttatgg
//
LOCUS       DBMDYA010000215         3731 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5684_length_3731_cov_1.567218, whole genome shotgun sequence.
ACCESSION   DBMDYA010000215 DBMDYA010000000
VERSION     DBMDYA010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5684_length_3731_cov_1.567218"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="ACZPFK_06380"
     CDS             <1..126
                     /locus_tag="ACZPFK_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1405971.1"
                     /translation="YPQVPPKVEYRLSETGQSLMPVLSALCRWGGAHMPQGWQLP"
     gene            complement(220..1347)
                     /locus_tag="ACZPFK_06385"
     CDS             complement(220..1347)
                     /locus_tag="ACZPFK_06385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1405972.1"
                     /translation="MQQLEQGDAARAVVERGVDVSKYQGRIDWRRVAAAGYRFAFVRT
                     GWAGYAGGIEEGYDPRFVENAVGAREAGLSVGAYVYSYMNTPAAAHAAAESVMRLVEP
                     YRLTYPLVLDFEDAKTYAALGRARNTAIARTFLEDLEAGGYYAMLYTYTNFANNYLDM
                     GALAAYDLWIADYRASVGYRGSYGIWQYTSSGRVNGITGAVDLDRSYKNYGSIIRGAG
                     LNHLTAPQPAMQPLTGAELEVFGRKNCEFFTAPDVYRVAGSLANGRYKAIARSTDVFG
                     GFSWVMLLMGGSVWWTALLEDRCRLIGGNGRSRSTLWEEERHLEELVDKYREENDFLR
                     CRLDAAEEKLSAARLLLDLPAPACAEQEAADRERVQNAESL"
     gene            1540..2760
                     /locus_tag="ACZPFK_06390"
     CDS             1540..2760
                     /locus_tag="ACZPFK_06390"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR1405973.1"
                     /translation="MKRLLSLTLALALALTVFAACGKDDSSSAASSGGGSSAPLATAT
                     PQPTPAPFDGQPLTGEEKGAGYVGGRPVAVMINNRSDSRPTRGLSQADVLYEIMVEGG
                     ITRFMALFPNANFSEIGPVRSGRDQFFRLIRPYQALYLFVGRSEITQQYIDDTEYGEL
                     AVNGMKVSFAYRDPVREAQGWTTDATAYTSGEKIQSYIQSNNIDMSRELSSPIFDFVD
                     YRESPRALGGESATSVNIVHSSSYRTAFDYDAASGKYLTSLYAPKSGYTPMVDEATGG
                     RMAFENVIVLFTDITQYPYPGGNPHGNPDYKKVDYDFGGLGYYISNGRIEKIRWTKGP
                     TDYPLMLQDEAGNSLKVNCGKSYVAVVSLNEYDNFSYEGDGGDATANSVAVDPNADSA
                     AQQAEQAADAADAS"
     gene            3021..>3731
                     /locus_tag="ACZPFK_06395"
     CDS             3021..>3731
                     /locus_tag="ACZPFK_06395"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 N-terminal domain-containing protein"
                     /protein_id="HIR1405974.1"
                     /translation="MTKRLFALLLCLALSLTALAGCGGGGNDEGGSQPAAATPAPTAT
                     PEPYDPNPLTGEAKGSDYVAVRPVAIMINNIAQARPQRGINDADVLYEIMVEGGITRF
                     MGLFNNYEALGDVGPVRSARDQFFRLVMPFQPLYVHIGRSGITQQYIDDVEYGDLNLD
                     GNGFDDLIYRDQGRLNQGYSYEHTAFTTGERLKSYVDWKDVDMSRDLSSPIFDFVDYR
                     EPARSLSGEDALSVGIVHS"
BASE COUNT          646 a         1342 c         1136 g          607 t
ORIGIN      
        1 tacccccagg tgccccccaa ggtggaatac cgcctgtctg agaccggcca aagcctgatg
       61 ccggtgctga gcgccctctg ccgctggggc ggcgcgcata tgccgcaggg ctggcagctg
      121 ccctgaccgc aaaggcaggc cccggccgtg ggacggccgg ggcctgcctt ttgtgaaaat
      181 tcgcccgcgg cgtaactttt tccgccgccc tctcgttaat tataaggatt cggcgttttg
      241 gacccgttcc cggtccgccg cttcctgctc cgcgcaggcg ggggcgggca ggtccagcag
      301 gaggcgggcg gcggagagct tctcctccgc cgcgtccagc cggcagcgca gaaagtcgtt
      361 ttcctcacgg tatttgtcca ccagctcctc caggtggcgc tcctcctccc acagggtgct
      421 gcgcgagcgc ccgttgccgc cgatcaggcg gcagcggtcc tccagcaggg ccgtccacca
      481 gacgctgccg cccatcagca gcatcaccca ggaaaagccg ccgaacacgt cggtgctgcg
      541 ggcgatggcc ttgtagcgcc cgttggccag gctgcccgcc acccggtaga cgtcgggcgc
      601 ggtgaaaaac tcgcagttct tccgcccaaa cacctccagc tcggcgcccg tcagcggctg
      661 catggcgggc tgcggcgcgg tcaggtggtt gagccccgcg ccgcggatga tggagccgta
      721 atttttgtaa gagcggtcca gatccaccgc gccggtgatg ccgttcaccc ggccgctgct
      781 ggtgtactgc cagatgccgt agctgccccg gtagcccacc gaggcgcggt aatcggcgat
      841 ccacagatcg taggcggcca gcgcgcccat gtcgagatag ttgtttgcaa agttggtgta
      901 ggtgtagagc atggcgtaat agccgcccgc ctccaggtcc tcgagaaagg tgcgggcgat
      961 ggcggtgttg cgcgccctgc ccagcgccgc ataggtcttg gcgtcctcaa aatccagcac
     1021 cagcgggtag gtcagccggt agggctccac caggcgcatc acgctctcgg ccgccgcgtg
     1081 ggcggcggcc ggggtgttca tatagctgta gacataggcc cccaccgaca ggcccgcctc
     1141 ccgcgcgccc acggcgttct ccacaaagcg ggggtcgtag ccctcctcaa tgccgcccgc
     1201 ataaccggcc cagccggtgc gcacaaaggc gaaccggtag cccgccgcgg cgacgcggcg
     1261 ccagtcgatg cggccctggt atttgctcac gtcgacgccg cgctccacca ccgcgcgcgc
     1321 ggcgtcgccc tgttccaatt gctgcatatc cttgcctcct tccgggggcc gcgccgggcg
     1381 cagctcccct gcctcagtct atccaccagg gcagaaaatg tttcccctgc ggcccggcat
     1441 tcactttaca tttgtcgtct ttttgggtat agtaaaaaag aaatcaaggc gcgggatgcg
     1501 taccccatcg tcaccgcgaa aagaaatgga gattttgcca tgaaacgtct gctctctctc
     1561 accctcgccc tggcgctggc cctgaccgtc tttgcggcct gcggcaagga cgattcctcc
     1621 tccgccgcct cctcgggcgg cggctcctcc gctccgctgg ccaccgccac gccgcagccc
     1681 acccctgccc cctttgacgg ccagcccctc accggcgagg aaaaaggcgc cggctatgtg
     1741 ggcggccggc cggtggcggt gatgatcaac aaccgctccg acagccgccc cacccgcggg
     1801 ctgtcgcagg ccgacgtgct gtacgagatc atggtcgagg gcggcatcac ccgcttcatg
     1861 gcgctgttcc ccaacgccaa cttctccgag atcggcccgg tgcgctcggg gcgcgaccag
     1921 ttcttccgcc tcatccggcc ctatcaggcg ctgtatctgt ttgtgggccg cagcgagatc
     1981 acccagcagt acatcgacga caccgagtac ggcgagctgg cggtcaacgg catgaaggtc
     2041 agctttgcct accgcgaccc cgtccgcgag gcccagggct ggacgaccga cgccaccgcc
     2101 tatacgagcg gggaaaagat ccagtcttac atccagagca acaacatcga catgagccgc
     2161 gagctgtcga gccccatctt tgattttgtg gactaccgcg agtccccccg cgccctcggc
     2221 ggcgagagcg ccaccagcgt caacatcgtc cactccagca gctaccgcac cgcgttcgat
     2281 tacgacgcgg cctcgggcaa atacctcacc agcctctacg cccccaagag cggctacacc
     2341 cccatggtgg acgaggccac cggcgggcgc atggcgtttg aaaacgtgat cgtgctgttc
     2401 accgacatca cccagtaccc ctatccgggc ggcaaccccc acggcaaccc ggactacaaa
     2461 aaggtggact atgactttgg cggcctgggc tactacatca gcaacggacg catcgaaaag
     2521 atccgctgga ccaaaggccc caccgactac ccgctgatgc tgcaggacga ggccggcaac
     2581 agcctgaagg tcaactgcgg caaatcctat gtggccgtgg tcagcctgaa cgaatacgac
     2641 aacttcagct atgagggcga cggcggcgac gccaccgcca actcggtggc cgtggacccg
     2701 aacgcggatt ccgccgcaca gcaggccgag caggccgccg acgccgcgga cgcctcctga
     2761 accgttttct ccgtatccgg cggcaagggg cccgatacct ctctggtatc gggccctttc
     2821 tttgcccgcc ggcgcggccc gcccccttgc tttgcggttt acaaagcggc ggcaaggttt
     2881 tataataggg atatcccatc ccctggcggc gggcgcgccg ccgcaaaaat acagccgggc
     2941 ccaaagccgg gccggacagg gcgctgctcc cgcgcccggc cccgccccga cacccgcccg
     3001 gcggaatcca ggagaaaaaa atgacaaagc gtctttttgc cctgctgctc tgcctcgcgc
     3061 tgtccctgac ggccctcgcg ggctgcggcg gcggcggtaa tgacgagggc ggctcgcagc
     3121 ccgcggccgc cacccccgcg cccaccgcca cgccggagcc ctacgacccc aacccgctca
     3181 ccggcgaggc caagggcagc gattatgtgg cggtgcgccc ggtggccatc atgatcaaca
     3241 acatcgccca ggcgcggccc cagcggggca tcaacgatgc ggacgtgctg tacgagatca
     3301 tggtcgaggg cggcatcacc cgctttatgg gcctgttcaa caactatgag gcgctgggcg
     3361 acgtgggccc ggtgcgctcg gcccgcgacc agttcttccg gctggtgatg ccctttcagc
     3421 cgctctacgt ccacatcggc cgcagcggca tcacccagca gtacatcgac gacgtggagt
     3481 acggcgacct caacctggac ggcaacggct tcgacgacct gatctaccgc gaccagggcc
     3541 gcctcaacca gggctactcc tacgagcaca ccgcctttac caccggcgag cggctcaaga
     3601 gctatgtgga ctggaaagac gtggacatga gccgtgatct gtccagcccc atcttcgact
     3661 ttgtggacta ccgcgagccc gcccgcagcc tctccggcga ggacgcgctc agcgtgggca
     3721 tcgtgcactc c
//
LOCUS       DBMDYA010000216         3723 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5695_length_3723_cov_1.749586, whole genome shotgun sequence.
ACCESSION   DBMDYA010000216 DBMDYA010000000
VERSION     DBMDYA010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_3723_cov_1.749586"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="ACZPFK_06400"
     CDS             complement(<1..601)
                     /locus_tag="ACZPFK_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1405975.1"
                     /translation="MKKITAMVLALAMALSLAACGGSDSSAAAGPASSGSSAAGPQGA
                     ASSTVAPAAGDWSDSERIAEIKAAGKIVLGTSADYPPFEFHTQVDGVDTIVGFDVALA
                     QKVADALGVELEVVDMSFDNLLISLDKGDFDFVMASLSNTPERAKAVDFSSSYFHGAQ
                     VVLVRAEDADKYTTKESLAGCQVAAQKGAIQVPLANEIAG"
     gene            complement(662..1702)
                     /locus_tag="ACZPFK_06405"
     CDS             complement(662..1702)
                     /locus_tag="ACZPFK_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014476.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR1405976.1"
                     /translation="MIHSIDPGILFLQEEDVVKAGALDMKMTIAAVEKTYALLGQGQV
                     INPPKTHIGMPIGSGANWNSFFNSMPCYIGGDINVGGTKWAAESKKNSAIPGIPYGID
                     ITVLSDPETVLPFCILDGTLITAMRTSAVAGVFAKHTAPADTKAVTLVGAGVIGRTMV
                     MAVCESLPQVKTIYLSDLDLAKAQGLAEDYKGVYDVQIVPTADAKAAALASQVIITET
                     TARKPFLDQSWVKPGVTVINMGSMEAELDVVTASDIIAVDYWDQIITYETKAAAQLYQ
                     AGKLTKDTVVELSDMVLGKWGGRTDPAQVINCSSLGLGALDIMIGYQLFLNAQKMGVG
                     TPLKLWEKPLWE"
     gene            complement(1770..2501)
                     /locus_tag="ACZPFK_06410"
     CDS             complement(1770..2501)
                     /locus_tag="ACZPFK_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1405977.1"
                     /translation="MIEVKGLRKSFGGNEVLQGLDAQIHRGEVVVVIGPSGSGKSTFL
                     RCLNRLEEPSGGQIIFEGSDICAKGADINLLRQKMGMVFQQFNLFNHLTVLENIVIGP
                     KLVKKCGPQQAEQEAMKLLERIGLADKAREYPSRLSGGQKQRIAIVRALAMNPDVMLF
                     DEPTSALDPEMVGEVLGVMKDLAGEGMTMVVVTHEMGFAQKVGTRVLFMDEGQILEEA
                     TPQKLFTAPRHPRTQDFLSKVLNCI"
     gene            complement(2506..3186)
                     /locus_tag="ACZPFK_06415"
     CDS             complement(2506..3186)
                     /locus_tag="ACZPFK_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1405978.1"
                     /translation="MDIIEIVTNYYQYFLQGTRTTILISLITVFCGFLLGCVISLLRL
                     SKNRLLNGFAKIYITVLRGTPLLVQLYIVYYQLDFIPYPKVSVWGIALERALPCVIAL
                     SMNSAAYVAEVIRSGIQAVDVGQGEAARSIGMTGAQTMLHIIMPQAIKNILPAIGNEF
                     VTMIKETSVIQYLGISDLMYNNGIVVTATYNPLPCYYISAVIYLILNLVAGKGVSIFE
                     RRLKRSDR"
BASE COUNT          685 a         1186 c         1122 g          730 t
ORIGIN      
        1 cgcccgcgat ctcgttggcc agcggcacct ggatcgcccc tttctgggcc gccacctggc
       61 agccggccag gctctctttg gtggtgtatt tgtccgcgtc ctcggcgcgc accagcacca
      121 cctgtgcgcc gtggaaatag gaggaggaaa aatccaccgc cttggcgcgc tccggcgtgt
      181 tggacagcga ggccatgaca aagtcgaaat cccccttgtc caggctgatg agcaggttgt
      241 cgaaggacat gtccacgacc tccagctcca cccccagcgc gtcggcgact ttctgcgcca
      301 aagccacgtc aaagcccacg atggtgtcga cgccgtccac ctgggtgtgg aactcaaacg
      361 gcgggtaatc cgccgaggtc cccagcacga tcttgccggc cgccttgatc tcggcgatgc
      421 gctcgctgtc ggaccaatcg ccggcggccg gggcaaccgt tgaggacgcc gccccctgcg
      481 ggccagccgc ggaggagccg gacgaagccg ggcccgccgc cgcgctggaa tcagagccgc
      541 cgcaggcggc cagggaaagc gccatcgcca gcgccaaaac cattgcagtg atctttttca
      601 tcttttctcc ctctctcttc tctccatctc cccgggcaat ctcccggggc gcaccggccc
      661 gtcactccca aaggggcttc tcccacagct tgagcggggt gccgacgccc atcttctgcg
      721 cgttcaggaa caactggtag ccgatcatga tatccagcgc gcccagcccc agcgaggagc
      781 agttgatcac ctgcgcaggg tcggtgcggc cgccccattt gcccagcacc atgtcggaca
      841 gctccaccac cgtgtccttg gtcagcttgc cggcctggta cagctgggcc gccgccttgg
      901 tctcataggt gatgatctga tcccagtagt ccacggcgat gatgtcgctg gcggtcacca
      961 cgtccagctc cgcctccatg ctgcccatgt tgatgaccgt gacgccgggc ttgacccacg
     1021 actggtccaa aaagggcttg cgggccgtgg tctcggtgat gatgacctgc gaggccagcg
     1081 cggccgcctt ggcgtcggcg gtgggcacga tctgcacgtc gtagacgccc ttgtaatcct
     1141 ccgccagccc ctgcgccttg gccaggtcta ggtcggacag gtagatggtc ttcacctggg
     1201 gcaggctctc gcagaccgcc atcaccatgg tgcgcccgat gacccccgcg cccaccagcg
     1261 tgacggcctt ggtgtccgcc ggggccgtgt gcttggcaaa gacgccagcc accgccgagg
     1321 tgcgcatggc ggtgatcagg gtgccgtcca ggatgcagaa gggcagtacg gtctcggggt
     1381 cgctgaggac ggtgatgtcg atgccgtagg ggatgccggg gatggcgctg ttctttttgg
     1441 actccgccgc ccacttagtg ccgcccacgt tgatgtcgcc cccgatatag cagggcatcg
     1501 agttgaaaaa cgagttccag ttggcgccgg agccgatggg catgccgatg tgggtcttgg
     1561 gcgggttgat gacctggccc tggcccagca gcgcataggt cttctccaca gccgcgatgg
     1621 tcatcttcat gtccagcgcg cctgccttga cgacgtcttc ctcttgtaaa aacagaatac
     1681 ccgggtcgat ggaatggatc atgattctct cctcctgtaa attgttgtcg ttttatcgta
     1741 tcgttttagg cggttatcgc gtccgcgcct cagatgcagt tgagcacctt ggacagaaag
     1801 tcctgtgtgc gcgggtgccg cggcgcggta aagagctttt gcggggtggc ctcttccaat
     1861 atctgtccct cgtccataaa gagcacccgg gtgcccacct tttgggcaaa gcccatctcg
     1921 tgggtgacca ccaccatggt catgccctcc ccggccaggt ctttcatcac ccccagcacc
     1981 tcgcccacca tctccgggtc cagggcgctg gtgggctcat cgaagagcat cacgtccggg
     2041 ttcatggcca gggcgcgcac gatggcgatg cgctgcttct gcccgcccga cagccgcgag
     2101 ggatattccc gcgccttgtc cgccagcccg atccgctcca gcagcttcat ggcctcctgc
     2161 tccgcctgct gcgggccgca ctttttcacc agcttggggc cgatgacgat attctccagc
     2221 accgtgagat ggttgaagag gttgaactgc tggaacacca tgcccatctt ctgccgcagc
     2281 agattgatgt cggcgccctt ggcgcagatg tcgctgccct caaagatgat ctggccgccg
     2341 ctgggctcct ccagccggtt gaggcagcgc aggaaagtgg acttgccgga tccggagggg
     2401 ccgatgacga ccaccacctc gccccggtga atctgcgcgt cgagcccctg cagcacctcg
     2461 ttgccgccaa agctcttgcg caggcccttg acctcgatca acacttcatc ggtcactgcg
     2521 cttcagcctc ctctcaaaaa tgctcacgcc cttgccggcc accaggttca aaatcaggta
     2581 gatcacggcc gagatgtaat agcagggcag cgggttgtag gtggcggtga ccacgatgcc
     2641 gttgttgtac atcaggtcgc tgatgcccag gtactggatg accgaggtct ccttgatcat
     2701 ggtgacgaac tcgttgccga tggcgggcag gatgtttttg atcgcctgcg gcatgatgat
     2761 gtgcagcatg gtctgggccc ccgtcatgcc gatggagcgg gccgcctcgc cctggcccac
     2821 gtccaccgcc tggatgcccg agcggatgac ctcggccaca taggccgccg agttcatcga
     2881 caaagcgatg acacagggca gggcccgctc cagcgcgatg ccccagacgc tcaccttggg
     2941 ataggggatg aaatccagct ggtagtagac gatgtacagc tgcaccagca gcggggtgcc
     3001 gcgcagcacc gtgatgtaga tcttggcaaa gccgttcagc agccgattct tcgacagccg
     3061 cagcagcgag atcacacacc ccagcagaaa gccgcaaaac accgtgatca gagaaataag
     3121 aatcgtcgtg cgggtgccct gtaaaaaata ttggtaataa tttgtaacga tctctatgat
     3181 gtccaagagg acgtcacctc ccaagcttgc tctcccgcgc ctttgcgttg ggtttattat
     3241 agcgatgttt gaataaatat gcaatatatt tttgctaaat tgtatatata ttcatataaa
     3301 tgcgtatttt actcgtgcta aaagtattaa tattcgtcaa agcgcacaag tattttcatc
     3361 cagcgcggcg catagcgcgc ccggcggagt ctttcagccc gcggatgaaa ggcttttctg
     3421 cgatgcggct ccgcgctgtg ccgtcactct gtggtataat cggtaccgtc gggacgcgcg
     3481 ccgcacagag ccgccgggga aaacgtgtat cgccgtgccc ggcggcgtga tcgccaccgc
     3541 tgcgggctgc gcagcagcca ccccggcaaa tgaccgcgga gggacaaaaa gcgagagcgt
     3601 gggcgggatt ggcgaaaacg gcctgcccaa gaaaacccgt caggccgcac aagcgcgctg
     3661 gggcaaggaa gcgcctccgt gaaaacgacg tgccgggcat agcgcgggcg gtcggaaacc
     3721 ggg
//
LOCUS       DBMDYA010000217         3686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5770_length_3686_cov_1.456346, whole genome shotgun sequence.
ACCESSION   DBMDYA010000217 DBMDYA010000000
VERSION     DBMDYA010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_3686_cov_1.456346"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="ACZPFK_06420"
     CDS             <1..2305
                     /locus_tag="ACZPFK_06420"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1405979.1"
                     /translation="CTLRLFRLACAGLGRRAPRLLQAPGLAGPLTAGLLRPVLLLPEK
                     AVGGEDLPLILAHELTHLRRHDLAKKALLLAANTLHWPNPAAWLLLRRAQRDIELACD
                     EAVTAGRGADYRDRYCGALLNSLAGARERALLPGARFARGKKEVRARLAHLFDRQPKR
                     RGRAAAALLLAAVVTCSALVACTAPESEEQLYYAPAPTAHGETQALVQWPLREAVFGI
                     CDLSSGDTGLYTGLMESEFLCKAPEGVTLASLPADALAGSYVRERGLLDLVYWSGDPS
                     SRLLRFYRSGDLGAAWESWELPCPSLGEREQISSLLSYERLNENSAFLSLLVTDEKGN
                     IRAPVFHSADGGKTWSPNGSAGPPHASQVRALRFVNEEVGFSVVEDVGQAQTLYLTLD
                     GGVTWEAPDLQPLLQLLPDPATEACSIWVEGARATIGYRTRSILNDGIQICTDDYGQS
                     WSWRARPEPLTGEWFSDQEIPAWMPLTKEFLDQYAPKSEPQEPQWAQRMWAYGARNGP
                     ETLLYACLSKPEIEQKAPPGLDEVIASGRGGLFPVTGSYIPEAGRWDLACLDPQAPDN
                     SILFYRSLDNAESWSSFSVKISDLPQGRSLSRVVYYEPLSEEIAFLSLTESVPGVENS
                     AVLCLYQTFNGGKTWSPRGELPLGADTVRHVRFLNDQVGFVTLLPGDSDSPRQLMRTL
                     DGGRSWEELSIDFSSLPEQRLFSCCLWVESDKVVVGFSSSRGFPDGYQLLSTDFGESW
                     SWREKPASPRPDGSPFTDLDDPAFLPI"
     gene            complement(2442..2855)
                     /gene="atpC"
                     /locus_tag="ACZPFK_06425"
     CDS             complement(2442..2855)
                     /gene="atpC"
                     /locus_tag="ACZPFK_06425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1405980.1"
                     /translation="MEQFSLQIVTPDGVSFSGEAERLIVRTIEGDVCILANHVQYLTA
                     LGTGTAKVTVGGETRRAACAGGMLAVTRSAEGASQVRLVATTFEWAEDIDAARAQRAK
                     ARAEELLQHAHDRHEQSVAQARLNRALTRLGAAGK"
     gene            complement(2898..>3686)
                     /gene="atpD"
                     /locus_tag="ACZPFK_06430"
     CDS             complement(2898..>3686)
                     /gene="atpD"
                     /locus_tag="ACZPFK_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1405981.1"
                     /translation="KTALVYGQMNEPPGARMRVGLSGLTMAEYFRDREHQDVLLFIDN
                     IFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGALQERITSTKHGSITSVQAVYVPAD
                     DLTDPAPATTFAHLDATTVLSRAISSLGIYPAVDPLESTSRILSPDVVGEEHYEVARE
                     VQRILQRYKELQDIIAIMGMEELSEDDKRTVSRARRIQRFLSQPFTVAEQFTGMQGKY
                     VPLKETIRGFKEIIEGRHDELPESAFLFAGTIDEVVEKAAAQPQ"
BASE COUNT          582 a         1266 c         1177 g          661 t
ORIGIN      
        1 ttgcactctg cggctgttcc gccttgcctg tgcgggcctg gggcggcgcg cgccgcggct
       61 gctgcaggcc ccgggccttg ccggacccct cacggccggg ctgctgcgcc cggtgctgct
      121 cctgccggaa aaagcggtgg gcggcgagga cctgcctctt attctcgccc atgagctgac
      181 ccatctgcgg cggcatgatc tcgccaaaaa agcgctgctg ctggccgcga acaccctgca
      241 ctggcccaac cccgccgcct ggctgctgct gcgccgggcc cagcgggaca ttgaactcgc
      301 ctgcgacgag gcggtgaccg ccgggcgggg cgcggactat cgcgaccgct actgcggcgc
      361 gctgctgaac tcgctggcgg gcgcgcgcga gcgcgcgctt ttgccgggcg cccgctttgc
      421 ccgcggcaaa aaagaggtcc gggcgcggct ggcccatctc tttgaccgcc agcccaagcg
      481 ccgcggcagg gccgctgcgg ccctgctgct ggcggcggtc gtaacctgca gcgccctggt
      541 ggcctgcacc gcgccggaga gcgaagaaca gctctattac gcccctgccc ccacggccca
      601 cggcgagaca caggccctgg tccaatggcc gctgcgggag gcggtttttg gtatttgcga
      661 tctttccagc ggggacaccg gcctgtatac cggcctgatg gaaagcgaat tcctctgcaa
      721 agcgccggag ggcgtcaccc tggccagcct ccccgccgac gctttggcgg gcagctatgt
      781 gcgagaacgg ggcctgctgg atctcgtcta ttggagcggc gacccctcct cccgcctgct
      841 gcgcttctat cggtccgggg atctgggcgc ggcctgggag agctgggagc tcccctgccc
      901 gtctttgggc gaaagggaac aaatctcctc cctcctgagc tatgaacgcc tcaatgagaa
      961 cagtgctttt ctctccctgc tagtgaccga tgaaaaaggg aatatccgcg ccccggtctt
     1021 tcacagcgcc gacggcggca aaacctggtc cccaaacggc tctgccggtc ccccgcacgc
     1081 ctctcaggtg cgcgcgctcc gcttcgtcaa cgaggaagtg ggattttcgg tggtggaaga
     1141 cgtcggccag gcacagacgc tttacctcac gctggacggc ggggtcacct gggaagcgcc
     1201 ggatctgcag ccgctgctgc agttgctgcc ggaccccgcg accgaagcct gctctatctg
     1261 ggtggaggga gccagggcca ccatcggcta ccgcacccgc agcatcctga acgacgggat
     1321 tcagatctgc accgacgact acgggcaaag ctggagctgg cgcgcacggc ccgaacccct
     1381 gacgggggaa tggttctccg accaggagat cccggcctgg atgcccctca caaaagagtt
     1441 tcttgaccag tacgccccaa aaagtgaacc gcaagaaccg cagtgggccc agcggatgtg
     1501 ggcctacggc gcccgcaacg ggccggagac cctgctttac gcctgcctgt caaaacccga
     1561 aatcgaacaa aaggcgccgc cgggacttga cgaggtgatc gccagcggcc gcggcgggtt
     1621 attcccggtc accggcagct atatccccga ggccggccgt tgggacctgg cttgcctgga
     1681 ccctcaagcc ccggacaaca gcatcctttt ttaccgctct ctcgacaatg ccgaaagctg
     1741 gagttctttt tcagtgaaaa tctccgacct gccgcaggga cggagcctct cccgcgtggt
     1801 ctattatgag ccgctcagcg aagagatcgc ttttttgagt ttgacggagt ccgtcccggg
     1861 tgtggaaaat tcagccgtcc tttgtctcta tcagactttc aacgggggaa aaacatggtc
     1921 gccgcgcggc gagctcccgc ttggcgcgga caccgtgcgc cacgtccgtt ttctcaacga
     1981 tcaggtaggg ttcgtcacct tgctccccgg cgacagcgac agcccgcgcc aactgatgcg
     2041 caccctcgac ggcgggcgca gctgggagga gctctcgatc gacttttcct ctctgccgga
     2101 acaaaggctt ttctcctgct gcctctgggt ggaaagcgac aaggtggtgg tgggcttcag
     2161 ctcctctcga ggattcccgg acggctatca gctgctgagc accgatttcg gcgagagctg
     2221 gagctggcgc gaaaagcccg cgtctccccg gcccgatggg tcgcctttca ccgatcttga
     2281 cgacccggcc tttctcccga tctgactcgg ggggcgcttt cctcgccctt ttgcagaagt
     2341 ccgaccggca caacctaaaa agatgggacc tgtcccagcc ctcacgcggc aaagacaggt
     2401 cccatctttt tccttttttt atgcagctat cgcgcggctc tctacttccc cgccgcgccc
     2461 agacgggtga gggcgcggtt cagccgggcc tgcgccaccg actgctcgtg gcggtcgtgg
     2521 gcgtgctgca gcagctcctc cgcgcgcgct ttggcgcgct gggcgcgggc ggcgtcgatg
     2581 tcctccgccc actcaaaagt ggtggccacc agccgcacct gcgaggcgcc ctccgcgctg
     2641 cgggtgaccg ccagcatgcc ccctgcgcag gcggcgcggc gggtctcgcc gcccaccgtc
     2701 accttggccg tgccggtgcc cagggcggtc aggtactgca catggtttgc cagaatacac
     2761 acgtcgccct cgatggtgcg gacgatcaac cgctcggcct cgccggaaaa gctgaccccg
     2821 tccggcgtca cgatctgcaa tgaaaactgt tccacagggg ccctcctccc gttttgtgcg
     2881 ccgcttggga cgccgcctta ctgcggctgc gccgccgcct tttccaccac ctcgtcgatg
     2941 gtgcccgcga acaggaacgc cgactccggc agctcgtcgt gcctgccctc gatgatctcc
     3001 ttgaagccgc ggatggtctc tttcagcggc acatacttgc cctgcatgcc ggtgaactgc
     3061 tcggccaccg tgaacggctg ggacaaaaag cgctggatgc gccgcgcccg ggacacggtg
     3121 cgcttgtcgt cctccgacag ctcttccatg cccatgatgg cgatgatgtc ctgcagctct
     3181 ttataccgct gcaggatgcg ctgcacctcg cgggccacct cgtagtgctc ctctcccacc
     3241 acgtcgggcg agaggatgcg gctggtggac tccagcgggt ccaccgccgg gtagatgccc
     3301 agcgaggaga tggcgcggga gagcaccgtc gtggcgtcca gatgggcgaa cgtggtggca
     3361 ggggccgggt cggtcaggtc gtcggcgggc acgtacaccg cctgcacgct ggtgatggag
     3421 ccgtgcttgg tggaggtgat gcgctcctgc agcgcgccca tctcggtggc cagggttggc
     3481 tggtagccca cagcgctggg catgcggccc agcagcgccg acacctcgct gcccgcctgg
     3541 gtgaagcgga agatgttgtc gataaaaagc aacacgtcct ggtgctcccg gtcgcggaaa
     3601 tactccgcca tggtgaggcc ggacaggccc acccgcatgc gcgcccccgg cggctcgttc
     3661 atctggccgt agaccagggc cgtctt
//
LOCUS       DBMDYA010000218         3678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5785_length_3678_cov_1.410400, whole genome shotgun sequence.
ACCESSION   DBMDYA010000218 DBMDYA010000000
VERSION     DBMDYA010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_3678_cov_1.410400"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(170..1327)
                     /locus_tag="ACZPFK_06435"
     CDS             complement(170..1327)
                     /locus_tag="ACZPFK_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405982.1"
                     /translation="MRKNKRIYDQNRIDSFFGALKIFCSLLIAIALIVVIVLIVSKQP
                     GQALRSFFVGPVDSTRHLANIVENMMPLLFTGVATCLMFAGGDVVLAGEGAFFLGAFL
                     SGAITTATPFSGPVKLALSLFAAMVGGAFVASVSIVAKNRFNAQPFVFSLLFNYVVMY
                     GCNFFLNYFLRDPDYIGIATYAFPADARFGAVVPKTSVTWGIFLAILAVVAGWFIMRK
                     TKLGYKASIVRDNPRYAINMGINAGRISLYMAILGGAVAGLGGCVEMLSKFTRYYWTT
                     QPGFGWDGFLLVTLAGNNPALIPFASLFLGYIRTGASVMNVFADVPMELISAIQAVMV
                     LLVASKALFGRTQQKVTRRNSELKSRMEAQGEKVIKAPEEQALTDSGGDRA"
     gene            complement(1314..2909)
                     /locus_tag="ACZPFK_06440"
     CDS             complement(1314..2909)
                     /locus_tag="ACZPFK_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405983.1"
                     /translation="MYALELKNITKVYGNGVLANDKVNFSVEKGEIHALMGENGAGKT
                     TLMKVLFGLEKPDEGEIVINGQKTEIQNPIDALRHGVGMVHQHFMLTPSLTVAENMIL
                     GSEPTRWKGLFIDRKKAVKSTREIAEKYKFNIDPTARVMDLPVGAKQQIEILKALYRG
                     AEILILDEPTAVLAPQETEKLFEQLKLLRQQGHTIVFISHKINEVIELCDRLTVMRQG
                     RSMGTYDIHGNGADGDQDITVDEISRLMVGRDAVLTVDKKNARPSQTVLCVDHVTKLN
                     QENRIAVDDVAFSVRAGEILAVAGVEGNGQRELVDMITGMTPIDIGDVRICGESIAGK
                     PLKYLHEERIAYIPQDRMASGVASGSSIMNNIIPYLIDKSDFEAFGVLKRKPLENISN
                     SVVAEFDIRCKDAQQKVGMLSGGNIQKVVVARELSDKCKQSVPLIVADQPSRGIDIGA
                     TKFIHRKLIELRDAGAAVLLVSADLNEVFELADSAIVMYEGKLAAYLPDMKQVDEFEL
                     GQYMLGVQTQGPEEIREVLYAEE"
     gene            complement(2994..3650)
                     /locus_tag="ACZPFK_06445"
     CDS             complement(2994..3650)
                     /locus_tag="ACZPFK_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1405984.1"
                     /translation="MFAGIRGLAARGKGLYGRIGFVGGQEVSSILDFCWGYVEVAKSV
                     NPDGKIVITFTNDWYDSAKAKEIAISEVNMGADVIFPAAGPSSEGVMEAASEKGIRSI
                     GVDEDREALYAKTNPDWVSSILTSEIKDVNAAVKRAIRMTLDGTLKYGESEALGLESG
                     CVGIIETGNYAQEVPDEVKALVEEAKKGALEGTLKITSAYGKSTEEVQKMKDWANGTD
                     "
BASE COUNT          766 a         1127 c          949 g          836 t
ORIGIN      
        1 aggttgccca tcagcgcact catcagcatg gaaccgtcta tgatgatatt taccgtgccc
       61 gattttgtag agatgagggc agccatcgag gcaaaaatca gcggggtggc catgcgaatg
      121 atcgaataaa aaaagttcgg cgtacagatc agttcaaata cttttccaat catgcccgat
      181 cacccccact atccgtcaag gcttgttctt caggggcctt tatcaccttt tccccctgcg
      241 cctccatgcg gctcttgagc tctgaattgc gccgggtgac cttttgctgt gtgcggccaa
      301 acagcgcttt ggaagcgacc agcagtacca tcaccgcctg aatcgcactg atcagctcca
      361 tgggcacatc cgcaaagacg ttcatgaccg atgcgccggt gcggatataa cccaggaaaa
      421 gggaagcgaa cgggatcagc gccgggttgt tccccgccaa ggtgacaagc aaaaagccgt
      481 cccatccaaa gccgggctgc gtcgtccagt aatagcgggt gaatttggac agcatctcca
      541 cgcagccgcc caggcctgca accgctccgc ccaggatcgc catgtacagg ctgatcctgc
      601 cggcattgat gcccatgttg atcgcatagc ggggattgtc gcggacgatc gaggccttat
      661 accccaattt tgttttgcgc atgatgaacc agccggccac caccgccaaa atcgccagaa
      721 atatgcccca ggtgacgctg gtcttgggca ccaccgcgcc aaagcgcgca tccgccggga
      781 acgcataggt ggcaatgccg atatagtccg ggtcgcgcag aaaatagttc aagaaaaagt
      841 tgcagccgta catgaccaca tagttgaaca acagagaaaa cacgaacggt tgggcgttga
      901 atctgttttt cgcgacgatc gagaccgagg cgacgaatgc tccgcccacc atggcggcaa
      961 aaagagagag cgccagcttg accgggccgc tgaacggggt ggccgtggtg atggccccgc
     1021 tcaaaaacgc ccccagaaaa aacgccccct cgcctgccag gaccacgtcg ccccccgcaa
     1081 acatcaggca ggtggcaacg cctgtgaaca gcaatggcat catattttcc acgatattgg
     1141 cgaggtgccg ggtggaatcg accggcccga caaaaaacga cctcagcgcc tggcccggct
     1201 gcttgctgac gatcaggacg atcaccacga tcagcgcgat cgcgatcaac agcgaacaaa
     1261 agattttcag cgccccgaaa aagctgtcga tccggttttg atcgtagatc cgcttattct
     1321 tccgcataaa gcacctctct tatctcctcc ggcccctgcg tctgtacccc cagcatgtac
     1381 tgtcccagct cgaactcgtc cacctgtttc atgtcgggca ggtatgccgc cagctttccc
     1441 tcatacatga cgatggcgga atccgccagc tcaaatacct cgttgagatc cgccgagacc
     1501 agcagcaccg cagcccctgc gtcgcgcagc tcgatcagct tgcggtggat aaattttgtc
     1561 gcgccgatat caatgccccg ggacggctgg tcggccacga tcagcggcac gctctgtttg
     1621 catttgtccg agagctcgcg cgccaccacg actttttgaa tattgcctcc ggagagcatc
     1681 cccacctttt gctgcgcgtc tttgcagcgg atatcaaatt ccgccaccac agagttggaa
     1741 atgttctcca agggtttcct tttcaacact ccaaaggctt caaaatctga tttatcaatc
     1801 aaatagggga taatattgtt catgatggaa gagccgctgg ccaccccgga cgccatgcga
     1861 tcctgcggaa tataggcgat gcgttcttcg tgcaggtatt tgaggggttt gcccgcaatg
     1921 ctctccccgc agatgcgcac gtcgccgata tcgatggggg tcattcccgt aatcatatcc
     1981 accagctcgc gctgtccgtt tccctcaacg cccgccaccg ccaatatctc gcccgcccgg
     2041 acagaaaagg ccacgtcgtc aaccgcgatg cggttttcct ggttgagctt ggtgacatgg
     2101 tccacacaga gcaccgtttg gctgggccgg gcatttttct tgtccaccgt gagcacggcg
     2161 tcgcgcccca ccatcaggcg ggaaatttca tccaccgtga tgtcctggtc gccgtcggcc
     2221 ccgtttccgt gtatgtcata ggtccccatc gagcgcccct gccgcatgac ggtgagccgg
     2281 tcacagagct cgatgacctc attgatctta tgcgaaataa agacgatcgt gtgtccctgc
     2341 tgccgcagaa gcttgagctg ttcaaacagc ttttccgtct cctgaggggc gaggacggcg
     2401 gtgggctcgt ccaggatcaa aatctcggcg ccccggtaaa gcgccttgag gatttcgatc
     2461 tgttgtttgg cacctacagg caggtccatc acccgcgcgg tggggtcgat gttgaatttg
     2521 tatttctccg caatctcacg ggtgctcttg accgcttttt ttcggtcgat aaaaaggcct
     2581 ttccagcggg tcggctcaga gcccaggatc atgttctccg ccacagtcag ggagggggtg
     2641 agcatgaaat gctggtgcac catgccgacg ccatgccgca gggcgtcgat ggggttttgg
     2701 atctccgtct tttgtccatt gatcacgatc tcgccctcgt ccggcttctc cagcccaaag
     2761 aggactttca tcagtgtcgt cttccctgcc ccattctccc ccatcagggc atggatctct
     2821 cccttttcga cgctgaagtt gaccttgtcg tttgcgagga cgccgttgcc gtaaactttc
     2881 gttatatttt tcagttccag agcatacatg ggcttttcac ctctgttttt aaacggagac
     2941 cggtttttta accggtctcc gtcccctttc tatttctggg cagcgcttcg cgctcagtcc
     3001 gtgccgttgg cccagtcttt catcttctgc acttcttccg tgctcttacc ataggcggag
     3061 gtgatcttta aagtcccttc cagggcacct ttctttgcct cttctaccag ggctttcacc
     3121 tcgtcgggga cctcttgggc ataatttccg gtctcgatga taccgacgca gccgctttcc
     3181 agacccaggg cttccgattc gccatacttc agcgtcccat ccagcgtcat acggatcgcg
     3241 cgcttgactg cggcgttgac gtccttgatc tctgaggtaa ggatggaact gacccaatca
     3301 gggttggtct ttgcatacag cgcctcgcgg tcctcatcca cgccgataga gcggattccc
     3361 ttttcggacg cagcctccat aacgccctca gaggaggggc ctgccgcggg gaaaatgaca
     3421 tctgcgccca tattgacctc tgaaatcgca atttccttgg cctttgcgga atcataccag
     3481 tcgttggtga aagtgatgac gatcttgccg tccgggttta cagattttgc gacctccaca
     3541 tagccccagc agaaatcgag gatagaggaa acctcctgtc cacccacaaa accgatcctt
     3601 ccgtagaggc ccttcccccg ggcagcgagc cccctgatgc cggcaaatat atcacgctca
     3661 cagacgggcg tctgctca
//
LOCUS       DBMDYA010000219         3675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5789_length_3675_cov_1.568551, whole genome shotgun sequence.
ACCESSION   DBMDYA010000219 DBMDYA010000000
VERSION     DBMDYA010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_3675_cov_1.568551"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="ACZPFK_06450"
     CDS             complement(<1..714)
                     /locus_tag="ACZPFK_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019845.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR1405985.1"
                     /translation="MKALVLNGVGKLDVEELQVGEPTGHEVVVDVKACGVCATDLHMY
                     HGDKGAFPNPIPLVMGHEFSGVVSQVGPEVTSLKVGDRVCVDPNMYCGHCRACRKGQV
                     HFCENMIGYGTTLYGGFAEQCRVLEHAAYKIPDSLSFEHAALVEPVACSLHGIDRSNI
                     HTGDTVAIIGLGSIGQLMLQLALAAGAARVVAIEPIASKRQTALKLGACLAIDPTAQD
                     VKKAVEEAGIESLDTVIECV"
     gene            complement(751..1731)
                     /locus_tag="ACZPFK_06455"
     CDS             complement(751..1731)
                     /locus_tag="ACZPFK_06455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1405986.1"
                     /translation="MIVTYRKYLEPAYGDPLPGTRIQAPEVCDMDDFGKWRKMHHLQT
                     IADLTNDIKSCYCYRPIMEIETTDTALERDGARIPLRVYHPEGEGPFPIMLFIHGGGW
                     RMNNLDIYDYVPRYFAKYGHVAVVSVDYRLAPEHKFPTGLEDAYAALEWAVANADSYR
                     GDIGSVSVCGDSAGGNFSAALCLMSRDRRGPAIHKQILLYPATTFLLNERTESEKHYG
                     NGDHFLIINSELGMVNDYFADQKDAEHPYASPLNAESLKGLPPACFISAECDPLLDQA
                     LMYAARLEDEGVEVEYHLYTGVLHAFINQTYQKTFEAMDDIIAAVPGSYR"
     gene            complement(1728..2723)
                     /locus_tag="ACZPFK_06460"
     CDS             complement(1728..2723)
                     /locus_tag="ACZPFK_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405987.1"
                     /translation="MKKQNVLNFIKEYTVVIVMVLLAAIFTIGNPAFLTASNFITILR
                     QSSMMGVAGIGVMFVMIAGGINLAVGSIISIVTVLVAMCCVNWGIHWVFGMVLSVIAA
                     TICGWFTGFAIVKGKIAPMIGSLAVKTILGGVAYIICGGLPVYGIPAESKVLGQGYIG
                     FVPVPVLIFVVIAAIAAFVLNKTYLGRYFYAAGSNDEAARLSGINTSKIRILAYTVSG
                     VLAGVAGIMMYGRVGSGQPIAGNEVEMNVLTAVVIGGVSMAGGEGKVFKACCGCVLIS
                     MLTNGLTLMNISEYTQMVIRGAIFLGAVILDSFQHRAKQQKAIKAVKQDKKEVKA"
     gene            complement(2739..>3675)
                     /locus_tag="ACZPFK_06465"
     CDS             complement(2739..>3675)
                     /locus_tag="ACZPFK_06465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1405988.1"
                     /translation="ELKAEGISIIFISHRLDELYRVADRVTVMRDGEVIITKEVSALS
                     KADLIYYMVGRELKESFPARSITYGDVALEVKGLTGNGVAPISFSVRRGEVVGLAGLV
                     GAGRTELSRLIFGADPKDGGEIFVDGKKVEIRSPRDAVDHGIGYVSEDRKQSGVLLRM
                     PISKNITLPILKRISHLGVVDTKKEAEVVKKQIGDMSIRTPSPEQLAGNLSGGNQQKV
                     VLGKWLASNSKILILDEPTRGIDVGAKQEIYKLINKLAEEGLAVIVISSEMEEVLGLT
                     DRMLILYEGQFMAELKKEDYSQERVLQFASGEQSV"
BASE COUNT          640 a         1107 c         1216 g          712 t
ORIGIN      
        1 cacgcactcg atgacggtgt ccagagactc gatgcccgcc tcttccaccg cttttttcac
       61 gtcctgggcg gtggggtcga tggccaggca ggcgcccagc ttgagggcgg tctgacgctt
      121 tgaggcaatg ggctcgatgg cgaccacccg ggcagcccct gcggcgaggg ccagctgcag
      181 catcagctgg ccgatgctgc ccagaccgat gatggccacc gtgtcgccgg tgtggatgtt
      241 gctgcggtcg atgccgtgca ggctgcaggc caccggctcc accagggcgg cgtgctcaaa
      301 ggacaggctg tcggggatct tgtaggccgc gtgctccagc acccggcact gctcggcaaa
      361 gccgccgtac agggtggtgc cgtagccgat catgttctca cagaagtgga cctggccctt
      421 gcggcaggcg cggcagtggc cgcagtacat gttggggtcg acgcagaccc ggtcgcccac
      481 cttgaggctg gtgacctcgg ggcccacctg ggagaccacg cccgaaaact cgtggcccat
      541 caccaggggg atggggttgg ggaaagcgcc cttgtcgccg tggtacatgt gcaggtcggt
      601 ggcacagacg ccgcaggcct tgacatcgac gacgacctca tggccggtgg gctcgcccac
      661 ctgcagctcc tccacgtcca gcttgcccac gccgttcaga actagtgctt tcatagtatc
      721 ttcctcgatt ctttgcgaaa ggttggcggc ctagcggtaa gacccgggca cggcggcgat
      781 gatgtcgtcc atagcctcaa aggttttctg ataggtctgg ttgataaagg cgtgcagcac
      841 gccggtgtag aggtggtact ccacctcgac gccctcgtcc tccagccggg cggcgtacat
      901 cagcgcctgg tccagcagcg ggtcgcactc ggccgagatg aagcaggcgg ggggcagccc
      961 tttcaggctc tctgcgttca gcggggaggc atagggatgc tccgcgtctt tctggtcggc
     1021 aaaatagtcg ttgaccatgc ccagctcgct gttgataatc agaaagtggt cgccgttgcc
     1081 gtaatgcttc tccgactcgg tgcgctcgtt gagcaaaaag gtggtggccg ggtagagcag
     1141 gatctgcttg tggatggcgg ggcctcggcg gtcccggctc atcaggcaga gagcggcgga
     1201 aaaattgccg cccgccgagt cgccgcagac cgagacgctg ccgatgtccc cgcggtagct
     1261 gtcggcgttg gccaccgccc attccagggc ggcgtaggcg tcctccagcc cggtggggaa
     1321 cttgtgctcc ggcgccagcc ggtaatccac cgagactacc gccacgtgtc cgtatttggc
     1381 aaagtagcgg ggcacatagt cgtagatgtc aaggttgttc atccgccagc cgccgccgtg
     1441 gatgaagagc atgatgggga aaggcccctc cccctcgggg tggtagacgc gcagcgggat
     1501 gcgcgcgccg tcccgttcca gcgctgtgtc ggtggtctcg atctccatga tggggcgata
     1561 gcagtagcag ctcttgatgt cgttcgtcag gtcggcgatg gtctgcagat gatgcatttt
     1621 gcgccacttg ccgaaatcgt ccatgtcaca gacctcgggg gcctggatgc gggtgccggg
     1681 cagggggtcg ccgtaggcgg gctccagata cttgcggtag gtgacgatca tgccttgacc
     1741 tcctttttgt cctgcttgac ggctttgatt gccttttgct gtttggcgcg atgctggaag
     1801 ctgtcgagga tgacggcgcc caggaagatg gcgccgcgga tgaccatctg ggtgtattcg
     1861 gaaatgttca tcagggtcag gccgttggtg agcatcgaga tcagcacgca gccgcagcag
     1921 gccttgaaga ccttgccctc gccgccggcc atcgagacgc cgccgatgac gacggcggtc
     1981 agcacgttca tctccacctc attgcccgcg atgggctggc cggagccgac acgcccgtac
     2041 atcatgatgc ccgcgacacc cgccagcacg ccgctgacgg tgtaggcgag gatgcggatt
     2101 ttgctggtgt tgatgcccga gaggcgggcg gcctcatcgt tgctgcccgc ggcgtaaaag
     2161 tagcggccca gataggtctt gttgagcacg aaagccgcga tggcggcgat gaccacgaag
     2221 atgagcacgg gcacggggac aaagccgata tagccctggc ccagcacctt ggactcggcc
     2281 gggatgccgt agacgggcag gccgccgcag atgatgtagg ccacgccgcc caggatggtc
     2341 ttgaccgcca gcgagccgat catcggggcg atcttgccct tgacgatggc aaagccggtg
     2401 aaccagccgc agatggtggc ggcgatgacg gagaggacca tgccgaagac ccagtggatg
     2461 ccccagttga cgcagcacat ggcgaccagc acggtgacga tggagatgat cgagcccacg
     2521 gcgaggttga tgccgccggc gatcatgaca aacatgacgc cgatgcccgc cacacccatc
     2581 atggaggact ggcgcaggat ggtgataaag ttgctggcgg tgagaaacgc cgggttgccg
     2641 atggtgaaga tggcggcgag cagcaccatc acgatgacga cggtgtactc tttgataaag
     2701 ttcaatacat tctgcttttt catttgtgct ctccttctct aaacagactg ctcgccggag
     2761 gcgaactgga ggacccgctc ctgggagtaa tcctcttttt tgagctctgc catgaactgc
     2821 ccctcataga ggatcagcat gcggtcggtc agacccagca cttcttccat ctcggaggag
     2881 atgacgatga cggcgaggcc ctcctccgcc agcttattga tgagcttgta gatctcctgt
     2941 ttggcgccca cgtcgatgcc gcgggtgggc tcgtcgagaa tgaggatctt ggagttgctg
     3001 gccagccact tgcccagcac caccttttgc tggttgccgc cggacaggtt gcccgccagc
     3061 tgctcggggc tgggggtgcg gatgctcatg tcgccgatct gttttttgac gacctcggcc
     3121 tcttttttgg tgtcgaccac gcccaggtgg gagatgcgct tgaggatggg caacgtgata
     3181 tttttgctga tgggcatgcg cagcagcacg ccgctctgct tgcggtcctc agacacatag
     3241 ccgatgccgt ggtccaccgc gtcgcggggg gagcggatct cgaccttttt gccgtccaca
     3301 aaaatctcgc cgccgtcctt ggggtcggcg ccgaagatca gccgcgacag ctcggtgcgg
     3361 cccgcgccca ccaggccggc cagccccacc acctcgccgc ggcgcaccga gaaagagatg
     3421 ggggcgaccc cgttgccggt gaggcctttc acctccagcg ccacgtcgcc gtatgtgatg
     3481 ctgcgcgcgg ggaaagactc tttcagctcg cggcccacca tgtaatagat caggtcggcc
     3541 ttggacagcg cgcttacctc tttggtgatg atgacctcgc cgtcgcgcat gacggtgacc
     3601 cggtcggcca cccggtacag ctcgtcgagc cggtgggaga tgaagatgat cgagatgccc
     3661 tcggctttca gctcg
//
LOCUS       DBMDYA010000220         3671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5797_length_3671_cov_1.530532, whole genome shotgun sequence.
ACCESSION   DBMDYA010000220 DBMDYA010000000
VERSION     DBMDYA010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5797_length_3671_cov_1.530532"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="ACZPFK_06470"
     CDS             <1..1002
                     /locus_tag="ACZPFK_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405989.1"
                     /translation="IFLFNYLVDPANVFYERYEETVADILLSGNNAEGVENMDDRRFL
                     RLYASSRTQPIDTLVLGSSRAMQITRAVTGDPNTFVAGVTGSDLRDCISAYFLFENEG
                     FRPKTVVLSLEFWYLSQGNMDTRALTEEYEIFCRKIGSTPVVTTSASLNKLKNFFSFT
                     YFQSSVEYLVKGKRKYLPFATTETNAVSAVKRVDGSYGYEESLREQSQAVIDSSARDK
                     QVVDNIAAGFSGVSPSLSEQLDAFVGYLQQQGIQVRLLLSPVHPLYYDYMLENPDVYS
                     IVFDSESFYRSVGGHYGVSVYGSFSPALVGANNYDFYDEVHPRESAVARYYNLEIKK"
     gene            1061..2749
                     /locus_tag="ACZPFK_06475"
     CDS             1061..2749
                     /locus_tag="ACZPFK_06475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR1405990.1"
                     /translation="MDDLPRYRLALLGDSATQHLATALKGDAQAQGIALELFEADYDQ
                     IQSQILDSDSSFYHFSPNAVLLYLCTEKLYERFVQYPLQQRDHFSHAVMEEILGYWNR
                     ISNTGNMKILQYNFVELDDRVFGDYAAKNEAAFISQLRRLNELLRAACVSHPEVFLLD
                     LQRIQNQLGRLNFYDPKLYYIAKMPISLMALPAAARCVTDVIMVLMGRVRKCIVLDLD
                     NTLWGGVIGDDGLNGIQLGELGLGRAYTDLQRWLKELRRRGVLLTVCSKNERETALEP
                     FEKHPEMVLRLEDIALFVANWEDKAANIRFIQQTLNIGMDSMVFLDDNPFEREAVRAL
                     IPDICVPELPEDPAQYLEYLRGLNLFNTLSYSDVDQIRTQQYQAESGRIQAQQQFATY
                     EDYLDSLEMTAVAAPFDSFHLPRAAQLTQRSNQFNLRTVRYTAADLQQLTQDPDYLTL
                     YFTLEDRFGDYGLIGVVILKRIDAQTAFVDTWLMSCRVLKRGMEEFIADKLVRTAQEH
                     GYRQVIGEYLPTVKNKMVSQLYEQMGFVPLGKGRYLADTEQYQPHHTKIKEKAL"
     gene            2746..2991
                     /locus_tag="ACZPFK_06480"
     CDS             2746..2991
                     /locus_tag="ACZPFK_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1405991.1"
                     /translation="MNRKEILKRLQPIFAEVFEDENIQLTEATTAADVDKWDSLSHLT
                     LIYEVESEFEMKFKMKEILEMKNVGEMVDILLREIKK"
     gene            complement(3116..>3671)
                     /locus_tag="ACZPFK_06485"
     CDS             complement(3116..>3671)
                     /locus_tag="ACZPFK_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014517.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HIR1405992.1"
                     /translation="LGDLIYSEESEGLENVVVPLLAGKRQRLATAESCTGGLLAQRIT
                     SVGGASEMFDFGAVTYASAIKRRILGVKPSTLKKYGAVSSVTAAEMAFGIQREAKADF
                     GVGITGIAGPGGGTADKPVGTVYVAVSTGHKVWVKRLSVEHSERSRVRRMSSQHALDM
                     VRRLALGLAIPDARCFGRHEAEEF"
BASE COUNT          891 a          915 c          889 g          976 t
ORIGIN      
        1 atctttcttt tcaactatct cgttgacccc gccaacgtct tttatgaacg ctatgaagaa
       61 acggtggcag acattctact ctctggcaac aatgcagagg gagtagaaaa tatggatgat
      121 cgccgttttt tgcgtctata cgcttcttcc cgcactcaac ccatcgacac tttggtgctg
      181 gggtcctctc gggccatgca gatcacccgt gctgttaccg gtgatcccaa tacctttgtc
      241 gcgggtgtga cgggttctga tttaagggat tgtatcagtg cttattttct ttttgagaac
      301 gagggattcc gtcccaagac ggtcgtgctc tcccttgaat tttggtacct ctctcaagga
      361 aatatggaca ctagggcgct cacagaggaa tatgaaattt tctgcagaaa aatcgggtcc
      421 actccagttg tgaccacttc ggcttcgctc aataaattga aaaacttctt ttcttttacc
      481 tattttcagt ccagtgtgga atatttagtc aaaggaaaac ggaaatatct gccctttgcc
      541 acaacagaaa cgaacgctgt ttccgctgtt aagcgtgtgg acggaagtta cggatacgaa
      601 gagagtctgc gtgaacaaag ccaggccgtg attgactcct ctgcccgtga caaacaggta
      661 gtggataata tcgccgccgg ttttagtggc gtctccccct ctctcagcga acaattagac
      721 gcatttgtcg gctatttaca gcagcagggc attcaagtgc gcttattgct ttctccagtc
      781 catcctcttt attatgatta catgctcgaa aatccggatg tttactccat cgtctttgac
      841 tccgaatctt tttatcgttc tgtgggaggg cactacgggg tatctgttta cggtagtttc
      901 tcccctgctc ttgtaggggc gaacaattat gatttttatg atgaagttca tccccgtgag
      961 agtgcggtag ctcgatatta taatcttgaa atcaaaaaat gaggtgagac caacggccgg
     1021 attgacattt tctcaattaa agaagaactg cgccgcccct atggacgact tgccccgcta
     1081 ccgtttggca ctgctgggag acagtgctac ccaacatttg gcgaccgctt taaaaggcga
     1141 tgcgcaggcg caggggattg ccctggagct ttttgaggca gactacgacc agattcaatc
     1201 tcaaatttta gacagcgatt ccagttttta tcatttttca ccaaatgctg tgcttcttta
     1261 tctctgcact gaaaagctgt acgagcgttt cgtccaatac ccgctgcagc agcgcgatca
     1321 tttttcccac gctgtcatgg aagagatttt aggctactgg aatcgtatca gcaacaccgg
     1381 aaatatgaag attctgcaat acaattttgt agagttggat gaccgggttt ttggagatta
     1441 tgccgccaaa aatgaagctg cctttattag tcagttgcgg cgccttaacg agttattgcg
     1501 ggccgcctgc gttagccatc cagaagtttt tttgcttgat ttgcaacgga ttcaaaatca
     1561 gcttggacgt ctcaacttct atgatcctaa actttactat atagcaaaaa tgccaatttc
     1621 cttgatggcg cttcctgccg ccgctcgctg cgtgacagat gtaatcatgg ttctgatggg
     1681 gcgcgttcgc aaatgtattg tgttggatct ggacaatacg ctttggggtg gagtcatcgg
     1741 agatgatgga ctgaacggca ttcagttggg agaattgggg ctgggacgtg cttatactga
     1801 cctccagcga tggctgaaag agctgcgccg tcgcggcgta ctgctgactg tgtgcagcaa
     1861 aaatgagcga gaaaccgcac tggagccctt tgaaaaacat cccgaaatgg tactacgcct
     1921 tgaagatatc gctctttttg ttgccaactg ggaggataaa gctgccaata tccgttttat
     1981 tcagcagacc ttgaacatcg gcatggatag tatggttttt ctagacgata atccgttcga
     2041 acgcgaagcg gtgcgggctc ttattcctga tatttgtgta ccagaattac ccgaggatcc
     2101 agcgcaatat cttgaatatc tgcgcggttt aaatcttttc aacactcttt cctattctga
     2161 cgtcgaccag atacgcactc aacaatatca ggctgaaagc ggtcgcattc aagctcagca
     2221 acagttcgct acctacgagg actatctgga cagcctcgaa atgacggctg tggctgcccc
     2281 tttcgactct tttcatctgc ctcgtgcagc acaacttacc cagaggtcta atcagttcaa
     2341 tctgcgcacc gtgcgctaca ctgcagccga cttgcaacag ttgacgcagg atcccgacta
     2401 tctgaccctt tattttaccc ttgaagaccg atttggcgat tacgggctga ttggggtggt
     2461 gattttaaaa agaatagatg ctcaaacagc ttttgtggat acctggttga tgagttgccg
     2521 tgtgctgaaa cggggaatgg aagaattcat cgccgacaag ttggttcgta ccgcgcagga
     2581 acatggttac cgtcaggtaa tcggcgaata tctgcccact gtaaaaaata aaatggtgtc
     2641 acagctttat gaacagatgg gttttgtccc tctgggaaaa ggtcgctatc tggctgatac
     2701 ggaacagtac cagccccatc atactaaaat aaaggagaaa gcgttatgaa ccgaaaagaa
     2761 attttaaagc gtttacagcc gatctttgca gaggtcttcg aagatgaaaa tattcaactt
     2821 acagaagcta ccacagcagc cgatgtcgac aaatgggatt ccctatccca cttgacactg
     2881 atttacgaag tggaatccga atttgaaatg aaatttaaaa tgaaagaaat cctggaaatg
     2941 aaaaatgtgg gggaaatggt ggatattctt ttgcgggaaa tcaaaaaata aacttctctt
     3001 tttagaggaa taaaaaaacg acgtcctgac cttaggtcac aaagggacgt cgttttacat
     3061 gtttacgttt tgttttttgt gagttcatac agataaaact atctttttat agtattcaaa
     3121 actcttccgc ctcatgcctg ccgaagcagc gggcgtcggg gattgccagg cccagtgcca
     3181 gacggcgcac catgtccagc gcgtgttggc tgctcatgcg ccgcacccgg ctgcgctccg
     3241 agtgctccac cgacaggcgt ttcacccaca ccttgtggcc ggtgctgacc gccacataaa
     3301 cggtgcccac cggcttgtcg gccgtgccgc cgcccgggcc cgcgatgccc gtgatcccca
     3361 ccccgaaatc ggcctttgcc tcccgctgga tgccgaaagc catctccgcg gctgtcacag
     3421 agctcacagc gccgtatttt ttcagcgtgg agggtttgac ccccaggatg cgtcgcttga
     3481 tggcgctggc ataagtcacc gcgccgaagt cgaacatctc ggaggcgccg cccacgctgg
     3541 tgatccgctg ggccagcagg ccgcccgtac agctctcggc cgtggccagc ctctggcgtt
     3601 tgcccgccag cagcggcacc accacgttct ccagcccctc cgactcctcg ctgtagatca
     3661 gatcccccag c
//
LOCUS       DBMDYA010000221         3671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5798_length_3671_cov_1.426050, whole genome shotgun sequence.
ACCESSION   DBMDYA010000221 DBMDYA010000000
VERSION     DBMDYA010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_3671_cov_1.426050"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="ACZPFK_06490"
     CDS             <1..676
                     /locus_tag="ACZPFK_06490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1405993.1"
                     /translation="AFQADRRATEMQRYYTLWAHQIKTPLAAMRLMISSADPAAFGPL
                     EDQLFSVERYVELVLQYQRLESISGDLDFVRCSLDDLARQAVRQYAPVFIRKGLSVRV
                     EGLQVEVLTDEKWAVFVIGQLLSNAVKYTQKGGVTLTLQPGSAATLVIRDTGVGIRPE
                     DLPRLGERGFTGENGRRDKLASGLGLFLCREVLAKLGHGFAIESRPGEGTAVKVDFAR
                     AALEIE"
     gene            831..1601
                     /locus_tag="ACZPFK_06495"
     CDS             831..1601
                     /locus_tag="ACZPFK_06495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1405994.1"
                     /translation="MPLLDVRNLKKIYATRFGGVQVQALTDVSFSVEQGEYVAIMGES
                     GAGKTTLLNILAALDKPTSGEVLLRSKNIVRIPDKQLSAFRREHLGFVFQDFNLLDTF
                     SVKDNILLPLVLSRKAVPEMEQRLAPIAAKLGITDILPKFPYEISGGQKQRAAVARAL
                     ITQPELVLADEPTGALDSKASGQLLRLLDEINAAGQTILLVTHSTRAASHAGRVLFIK
                     DGEVFHQLYRGGASNEVMFQRISDTLTALATGGDHFEQ"
     gene            1661..3646
                     /locus_tag="ACZPFK_06500"
     CDS             1661..3646
                     /locus_tag="ACZPFK_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1405995.1"
                     /translation="MRNLPFYPRLALTNLKNNRQTYYPYLLTCILCVMTFYTVLSISA
                     NSSFENLPSAAWVQMVMIQGVFIIAIFSAILIFYTNGFLIKRRKRELGLYNILGMEKR
                     HVGRMMLFETLFTALFAIAVGLLLGIVFSRVMFLILLRMLHLGSSIAFQLSPAMLIVT
                     AVLFAVIFLFTLLSNLRQVRLANPVELLHGGQMGEREPKTSWPLAIGGLLCLGGGYAI
                     AVTVKSPAAAILLFFLAVMLVIVGTYALFTAGSIALLKALRKNKKFYYRPRPFIAVSG
                     LIYRMKQNAKGLASICILSTMVILTIATTLSLYAGQEGMLKREFGREVTVSARPSEGG
                     LSEEEVNALVDETLAEAGVQAIHRNTYRYTAFHAQKEGGLLSPKQDLGPTAPLLSLTV
                     LPLEDYNRMAGQNRTLAPSQALVFTDGTPYGSGTVTLGESTWYVAEELSSFPLAERTR
                     ASLVSSYTGECYIVLPTADEVLTLSHLYGGNAGDQLRRACTFDLVGDDAAKTAFASRL
                     QEKLLPLPGTSMNSVHLVREYWYSLYGGFFFLGLFLGALFMMATALIIYYKQLSEGFE
                     DRGRFLILQQVGMSQKEVKSTIRSQILMVFFLPLAMALLHTAFAFPVMRTILTAFGLA
                     DLMLFFLCTAGTALVFSLLYFFTYSLTARTYYKLVRE"
BASE COUNT          622 a         1224 c         1128 g          697 t
ORIGIN      
        1 tgcctttcag gcggaccgcc gggccaccga gatgcagcgc tattacacgc tgtgggcgca
       61 ccagatcaag accccgctgg cggccatgcg gctgatgatc tcctccgccg acccggccgc
      121 tttcggcccg ctggaggacc agctcttttc cgtagagcgg tatgtggagc tggtgctgca
      181 ataccagagg ctggagagca tctccggcga tctggacttt gtgcgctgca gcctggacga
      241 cctggcccgg caggcggtgc gccagtacgc gcccgtcttt atccgcaagg ggctctcggt
      301 gcgggtggag gggctgcagg tggaggtgct caccgacgaa aagtgggcgg tcttcgtgat
      361 cggccagctg ctctcgaacg ccgtgaaata cacccaaaag ggcggcgtga ccctgacgct
      421 gcagcctggc agcgcggcga cgctggtgat ccgggacacg ggggtgggca tccgccccga
      481 ggacctgccc cgtctgggcg agcggggctt caccggcgaa aacgggcggc gggacaagct
      541 ggccagcggg ctggggctgt ttctctgccg cgaggtgctg gcaaagctgg gccacggctt
      601 tgccatcgag agccgcccgg gcgagggcac ggcggtcaag gtggattttg cccgcgcggc
      661 gctggagatc gagtgagcgg cccgcaccgg gcgcggccgg gcgcagcctt acaaaactgt
      721 aagaggcagg ggcggaactg taagccggaa cgatggctgc ggggccgctc tttttttata
      781 atagaatcac agggcgcagc cctcacatct tttacaagga ggcagttttc atgccgcttt
      841 tggacgtacg caacctcaaa aagatctatg ccacccgctt tggcggcgta caggtacagg
      901 ccctcaccga cgtgagcttt tcggtggagc agggggaata tgtggccatc atgggcgagt
      961 cgggcgcggg caagaccacg ctgctcaaca tcctggccgc gctggacaag cccaccagcg
     1021 gcgaggtgct gctgcgcagc aaaaacatcg tgcgcatccc cgacaagcag ctctccgcgt
     1081 tccggcgcga gcatctgggc tttgtgtttc aggatttcaa cctgctggac accttttcgg
     1141 tgaaagacaa tatcctgctg ccgctggtgc tctcccgcaa ggcggtgccg gagatggagc
     1201 agcggctcgc ccccatcgcg gccaagctgg gcatcaccga catcctgccc aagttcccct
     1261 acgagatctc gggcgggcag aagcagcgcg ccgccgtggc ccgcgccctg atcacccagc
     1321 cggagctggt gctggccgac gagcccaccg gcgcgctgga ctcgaaagcc agcgggcagc
     1381 tgctgcgtct gctggacgag atcaacgccg ccgggcagac cattttgctg gtcacccaca
     1441 gcacccgggc cgccagccac gccgggcggg tgctgttcat caaggacggc gaggtctttc
     1501 atcagctcta tcggggcggc gcctccaacg aggtgatgtt ccagcgcatc tcggacacgc
     1561 tgaccgcgct ggcgacggga ggggatcact ttgaacagta aagctttcac gcccctattc
     1621 gacgccgctc cccaccgccc tgcgagaggg ggtgccggcc gtgcgtaacc tgccctttta
     1681 cccgcgcctc gccctcacca acctgaaaaa caaccggcag acctattacc cttatctgct
     1741 cacctgcatc ctctgcgtga tgacctttta cacggtgctc tcgatctcgg ccaactccag
     1801 cttcgagaac ctgcccagcg ccgcctgggt gcagatggtg atgatccagg gggtcttcat
     1861 catcgccatc ttttccgcca tcctcatctt ttacaccaac ggctttctca tcaaacgccg
     1921 caaacgggag ctggggctgt acaacatcct gggcatggaa aagcgccatg tgggccgcat
     1981 gatgctgttc gagacgctgt tcacggcgct cttcgccatc gccgtcggcc tgctgctggg
     2041 catcgttttc agccgcgtga tgttcctcat cctgctgcgc atgctgcatc tgggcagctc
     2101 catcgccttt cagctctcgc ccgccatgct catcgtcacc gccgtgctct ttgccgtcat
     2161 ctttttgttc acgctgctgt ccaacctgcg gcaggtgcgt ctggcaaacc ccgtggagct
     2221 gctgcacggc ggccagatgg gcgagcggga gcccaaaacc tcctggccgc tggccatcgg
     2281 cgggctgctc tgcctgggcg gcggctacgc catcgcggtg acggtgaaga gccccgccgc
     2341 ggcgatcctg ctgttttttc tggccgtgat gctggtcatc gtcggcacct atgccctctt
     2401 caccgccggc agcatcgcgc tgctcaaggc gctgcgcaaa aataaaaagt tctattaccg
     2461 tccgaggccc tttatcgccg tgtccggcct catctaccgc atgaagcaga acgccaaggg
     2521 cctggccagc atctgcatcc tctccaccat ggtcatcctc accatcgcca ccaccctgtc
     2581 cctctacgcc gggcaggagg gcatgctgaa acgcgagttc gggcgcgagg tcaccgtgtc
     2641 ggcccgcccc tccgagggcg gcctgtcgga ggaggaggtc aacgcgctgg tggacgagac
     2701 gctggccgag gcgggcgtgc aggccatcca ccgcaacacc taccgctata ccgcttttca
     2761 cgcccaaaaa gagggcggcc tgctctcgcc caaacaggac ctgggcccca ctgcgccgct
     2821 gctctcgctc accgtgctgc cgctggagga ttacaaccgc atggccgggc aaaaccgcac
     2881 cctggccccc agccaggccc tggtcttcac cgacggcacg ccctacggca gcggcaccgt
     2941 gaccctgggg gagagcacct ggtacgtggc ggaggagctt agcagctttc cgctggccga
     3001 gcgcacccgc gcctccctgg tcagctccta caccggcgag tgctacatcg tgctgcccac
     3061 cgccgacgag gtgctcaccc tctcgcacct ctacggcggc aacgcgggcg accagctgcg
     3121 ccgcgcctgc actttcgatc tggtggggga cgacgcggcc aaaaccgctt tcgccagccg
     3181 cctgcaggaa aagctgctgc ccctgccggg cacctcgatg aactcggtcc atctggtgcg
     3241 cgagtactgg tattcgctgt acggcggctt tttctttctg gggttgttcc tgggggcgct
     3301 gttcatgatg gcgacggccc tcatcatcta ctacaagcag ctgtccgagg gctttgagga
     3361 ccggggccgc tttttgatcc tgcagcaggt gggcatgagc caaaaggagg tcaaatccac
     3421 catccgcagc cagattttga tggtgttctt tttgcccctg gcaatggcgc tgctgcacac
     3481 ggcgttcgct ttcccggtga tgcgcaccat cctcaccgct ttcggcctcg cggacctgat
     3541 gctctttttc ctgtgcaccg cgggcacggc gctggtcttt tcgctgctct atttcttcac
     3601 ctattccctc accgcccgca cctattacaa gctggtgagg gaataggtga agaaatagag
     3661 cagcgaaaag a
//
LOCUS       DBMDYA010000222         3670 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5801_length_3670_cov_1.234519, whole genome shotgun sequence.
ACCESSION   DBMDYA010000222 DBMDYA010000000
VERSION     DBMDYA010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5801_length_3670_cov_1.234519"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="ACZPFK_06505"
     CDS             complement(<1..190)
                     /locus_tag="ACZPFK_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405996.1"
                     /translation="MKRQKLIAITLAVLLLAVSLVSCFASAGDGAASGAPSSAPAPTA
                     APTEDQSADIVVIGGGGAG"
     gene            complement(553..1830)
                     /locus_tag="ACZPFK_06510"
     CDS             complement(553..1830)
                     /locus_tag="ACZPFK_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR1405997.1"
                     /translation="MKKHRILEPAEILLPRADTDMEKWACLACDQFTSQPDYWRRAEA
                     LVGDAPSCLKLVLPEVYLEQPGVERRIGEIHRAMRHALAHTLTRAVQGFVYVRRTTGS
                     GVREGLVAAVDLEQYSYAKGAQPLIRPTENTVESRIPPRLAVRRGAALEAPHILMLVD
                     DPQNTLLGPLAAARLELPLLYDTPLMLGGGRIEGRAVTDPALVEGAIAAVEAFAARPG
                     PGGRSPIAIAVGDGNHSLATAKAWWEEVKAGLSPAEAESHPARYCLVELENIHDPAIL
                     IEPIHRVVFGARPDGFVAEMERFFAAHGGALVRADDAEAAGQRFTLATAGGDRRYAVK
                     HPAWPLCVGSLEAFLSGYLPAHPEARVDYIHGEAAVRELAAGGALGVLLPPFEKADLF
                     RGVYLGGVLPKKTFSMGHAEEKRYYLECRRITR"
     gene            2132..3004
                     /locus_tag="ACZPFK_06515"
     CDS             2132..3004
                     /locus_tag="ACZPFK_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1405998.1"
                     /translation="MEQCGGQAGRGRLKVLAAALMFSISGVCVKLIPWSPLAINGARG
                     AVGALVMGLWFVGAKHRFRLNGAVLTGALALCLTNILYVFSTKLTTAANAILLQYTSP
                     IFVVLLLWLGFGKRPRKLDLVTCFFVFCGILFFFFDSLTADSLLGNFLGLASGLTYAG
                     VFLVNLMPGADSMSSFLLAQLVGAALGAPFLLREHSFSPAALAAVALMGVFQIGLGFL
                     LLSQGLVHTPAVTANLICAVEPILNPTLVALLYGERPGRFALVGAVIVLAGVIGYNVM
                     NGRQSGRAARREMK"
     gene            complement(3381..>3670)
                     /locus_tag="ACZPFK_06520"
     CDS             complement(3381..>3670)
                     /locus_tag="ACZPFK_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1405999.1"
                     /translation="LRVADPDHGRIAPLLRSGAADGKGLPVEQPGAKFHAGQLGFCIL
                     YSGAGCCSGGRALALADYTRRDAVTGRYALETLPLTEFFAAAGQAPGPRDP"
BASE COUNT          547 a         1199 c         1282 g          642 t
ORIGIN      
        1 tgcccgcgcc gccgccgccg atcacgacga tatcggcgct ctggtcctct gtgggcgcgg
       61 ccgtgggggc gggggccgac gacggcgcgc ccgaggccgc gccgtcgccc gcgctggcaa
      121 agcagctgac caggctgacg gccagcagca gaacggcgag agtgatcgca atgagttttt
      181 gtctcttcat gcttttctcc tcttgtgtga aaaatgtgaa agaatgcgat tcccattgta
      241 cgggaaaaac ggcttcaatt caaccacaaa gccctgacaa ttttttatca gctggaattt
      301 tcagaaacag gcacaaaaaa taaaaaaatt ccccgctttt gcctgtcgaa aaagggaagc
      361 cctgccggtc ggccaggggg ctggtcggca cgggagaggg agaaaaatgg gagagcggcc
      421 ttggggctgc tctcccgcag ggagtgtcgg cgaagaacat gcggtggccc ggtcgcccag
      481 ggcgttctcc cgacagctgc gggacgcgcg ccggtctgtg agaggccaaa atctctctgc
      541 gcctgcgacg cctcagcgcg tgatgcggcg gcactcgaga tagtagcgtt tttcctccgc
      601 gtgccccatg ctgaacgtct ttttcggcag cacgccgccc agatagacgc cgcggaagag
      661 gtccgccttt tcaaacggag gcagcagcac ccccagcgcg ccgccggccg ccagctcccg
      721 caccgccgct tcgccgtgga tatagtccac ccgcgcctcg ggatgggcgg gcagatagcc
      781 ggataaaaag gcctccaggc tgcccacaca caggggccat gcgggatgct tgacggcata
      841 gcgccggtcg ccgcccgcgg tcgccagcgt aaagcgctgg cccgccgctt cggcgtcgtc
      901 ggcgcgcacc agcgccccgc cgtgagccgc aaaaaagcgc tccatctcgg cgacgaaccc
      961 gtccggccgg gcgccgaaca ccacccggtg gatgggctct atcaggatgg cggggtcgtg
     1021 gatgttctcc agctccacca gacagtagcg ggcggggtga ctctcagcct cggcggggga
     1081 gagcccggcc ttgacctcct cccaccaggc cttggcggtg gccagcgagt ggttgccgtc
     1141 tcccaccgcg atggcgatgg ggctgcggcc cccggggccc ggccgcgcgg caaaggcctc
     1201 cacggcggcg atcgcgccct ccaccagcgc cgggtcggtc accgcgcggc cctctatgcg
     1261 ccctcctccc agcatcaggg gggtgtcgta gagcaggggc agctccagcc gcgctgcggc
     1321 cagggggccg agcagggtgt tttgggggtc gtccaccagc atcaggatgt ggggcgcttc
     1381 cagcgcggcg ccccggcgca cggccaggcg gggcgggatg cggctctcca ccgtgttttc
     1441 cgtggggcgg atcaggggct gcgccccttt ggcgtagctg tactgctcca ggtcgacggc
     1501 cgcgaccagc ccctcccgca cgccgctgcc ggtggtgcgg cgcacgtaca caaacccctg
     1561 taccgcccgg gtcagggtgt gggccagcgc atggcgcatg gcgcggtgga tctccccgat
     1621 gcggcgctcc acccccggct gctccagata gacctccggc agaaccagct tcaggcagct
     1681 gggggcgtcc cccaccaggg cttcggcccg ccgccagtag tccggttggg aggtgaactg
     1741 gtcacaggcg aggcaggccc atttttccat gtccgtgtcc gcgcggggca gcaggatctc
     1801 ggcgggctca aggatgcggt gttttttcat ggtttccctc tctcttttgc ggtttgcggc
     1861 ggccgtgttt tttcaaaacg gggaaagggc ctccctttcc ggaggccgcc ggcgggcgcc
     1921 tgtgggcagg gcgctgtccg ccgtgtcctg tcgagacgcg gctgttgccg cgttcggtgt
     1981 tacggcggcg gcctggccgt cggggggacg ggggcgcggc gtccggcaag gacagctctt
     2041 ctagttttcg gcgcggccta atatctttaa attataaggc cggggctgcg gttttgcaag
     2101 aaagcggcgg cgggcgaaag gggcgggcgg catggagcag tgcgggggac aggcggggcg
     2161 cggcagatta aaggtattgg cggcggcgct gatgttcagc atcagcgggg tctgcgtcaa
     2221 gctcatcccc tggtcgcccc tggccatcaa cggcgcgcgc ggggcggtgg gggcgctggt
     2281 gatgggactc tggtttgtgg gcgcaaagca ccgcttccgg ctcaacgggg cggtgctcac
     2341 cggcgcgctg gccctctgcc tgaccaatat cctctatgtc ttttccacca agctcaccac
     2401 ggctgccaac gccattctgc tgcagtacac ctcgcccatc tttgtggtgc tgctgctctg
     2461 gctgggcttt ggcaagcggc cgcgcaagct ggatctggtc acctgctttt tcgtcttctg
     2521 cgggattttg ttcttttttt tcgactccct cactgccgac agcctgctgg gcaattttct
     2581 gggcctcgcc agcggcctca cctatgccgg cgtctttctg gtcaacctga tgcccggcgc
     2641 ggacagcatg tcctcttttt tgctggccca gctggtgggg gcggcgctgg gggcgccgtt
     2701 tctgctgcgg gagcacagct tttcgcccgc ggccctggcg gcggtggcgc tgatgggggt
     2761 gttccagatc gggctgggct ttctgctgtt gtcccagggg ctggtccaca ccccggcggt
     2821 caccgccaac ctgatctgcg cggtcgagcc catcctcaac cccaccttgg tggccctgct
     2881 ctacggcgag cgcccgggcc gcttcgcgct ggtgggggcg gtgatcgtgc tggcgggagt
     2941 gatcggctac aacgtgatga acggcaggca gagcggccgg gccgcccgca gagaaatgaa
     3001 ataaacggcg cttaagcgca tggcgataaa aagaggccgc cgcccgggcg gggagccata
     3061 tgcagaacgc cgcggggccc gcttcacggc ggctgaacgg ccgatcttcc gcgcgacgcc
     3121 gcagagggag gggcgaggga ggcgcgccaa ggggcggaga caaacacgcg gttttctccg
     3181 ccgccgccca gggcccctct tttaggaggc gggcagcggt ttttgcccgc cccgcggcgc
     3241 tggagaggca cacggtgaaa aaaagatcgg cgatccgcgg gggtcgccga tcttttcaaa
     3301 tgcgcccggg ggcggttctg cggggcagac cgccgtttgt tttgcggcag cgcgcctttc
     3361 catcccctgc cccaggcggt tcaagggtcg cgcgggccgg gggcctgtcc tgcggccgcg
     3421 aagaattctg tcaagggcag cgtttccagc gcatagcggc cggtcactgc gtcgcggcgg
     3481 gtgtagtcgg caagggccag cgcccggccg ccgctgcagc agcccgcccc gctgtaaagg
     3541 atgcaaaagc ccaattgccc ggcgtggaat ttcgcccccg gctgctccac cggcagcccc
     3601 ttgccgtccg ccgcgccgga gcgcagcagc ggcgcgatgc ggccgtgatc tgggtccgcc
     3661 acccgcaaaa
//
LOCUS       DBMDYA010000223         3667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5808_length_3667_cov_1.720135, whole genome shotgun sequence.
ACCESSION   DBMDYA010000223 DBMDYA010000000
VERSION     DBMDYA010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_3667_cov_1.720135"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="ACZPFK_06525"
     CDS             <1..1099
                     /locus_tag="ACZPFK_06525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HIR1406000.1"
                     /translation="EYRAVWLTYLEYQGMDMSSEASFRTAVATAFDQIAALGANTVVA
                     HVRPFSDALYRSALFPWSHLLTGAQGADPGYDPLAVMVEEAHGRGLRLEALVNPYRIR
                     GAGGAPETLAEGNPAALYAADPEKSGWVLQQDGGTYYNPAVPEVRQLIVDGVREICQN
                     YQVDGVQFDDYFYPEGADDSFDQAAYERLANGADRGDWRRENVNALVRDAYAAVKAVN
                     PSLTFGISPQGNNDNNYNIQYSDVALWMAESGYVDYVMPQIYWGFDYRTGSGRDDFAF
                     ENCLAGWLALPRAQGVKLYVGLGAYRIGAGDGGSNDQAEWSSGSNLARQVRALRQAGA
                     DGYGLYSYRWLFQNDQPTVAAEVEALTGANG"
     gene            1218..1877
                     /gene="trmB"
                     /locus_tag="ACZPFK_06530"
     CDS             1218..1877
                     /gene="trmB"
                     /locus_tag="ACZPFK_06530"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1406001.1"
                     /translation="MRMRHKAYARPELEACPFFIGDPERWKGRWQKAFDQPRPLVLEL
                     GCGKGAYAAAMAAAHPEINYLAVDYLSDVLVLAKRRVEEAYAAAGRPVDNCRLTALNI
                     QWIDADLGPEDVVERIHINFCNPWPKFGHKKRRLTHPRQLQHYRAFLRDGGEVWFKTD
                     DDGLYRESLEYFAQCGFSLRYQTLDLHAQEPSWNLRTEYEERFSAQGVPIKALIAVKE
                     P"
     gene            2176..2622
                     /locus_tag="ACZPFK_06535"
     CDS             2176..2622
                     /locus_tag="ACZPFK_06535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1406002.1"
                     /translation="MLEVKFYEEGEALGPKLRFAVVAALCGGRWVLCKHKTRATWEFP
                     GGHREPGEPIAETARRELYEETGALDYRLRPVCAYGVRDPQGAGEETFGMLYLAEISR
                     FGPLPAEFEMERIQLFDRPPQAPAQWTYPEIQPPLLERLGRELARE"
     gene            3313..>3667
                     /locus_tag="ACZPFK_06540"
     CDS             3313..>3667
                     /locus_tag="ACZPFK_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406003.1"
                     /translation="MDRKILVDQSIDFIMQHLNEPLSLDTVADQVHLSKYHFSRIFKE
                     ETGESVYAFIKRCKVDQSAIDMKLNPTKPIIEIGLDYGYSASNYSSLFRKHHDTSPTM
                     FRQSIPTDGMAVPFAP"
BASE COUNT          752 a         1032 c         1220 g          663 t
ORIGIN      
        1 ggaataccgc gccgtctggc tgacctacct ggaatatcag ggcatggata tgagcagcga
       61 ggcctctttc cgcaccgcgg tggccacggc gttcgatcag atcgcggccc tgggggcgaa
      121 tacggtggtg gcccacgtgc ggcctttcag cgacgcgctc taccgcagcg cgctcttccc
      181 ctggagccat ctgctcaccg gcgcccaggg cgcggacccg ggctacgacc cgctggcggt
      241 gatggtggag gaggcgcacg gccgagggct gcggctggag gcgctggtca acccctaccg
      301 catccgcggc gccggtggcg cgccggagac gctggcggag ggcaaccccg ccgccctcta
      361 cgccgccgac ccggaaaaaa gcggctgggt gctgcagcag gacggcggca cctattacaa
      421 ccccgccgtc cctgaggtgc gccagctgat cgtggacggc gtgcgggaga tctgccagaa
      481 ctatcaggtg gacggcgtgc agttcgacga ctatttttat cccgagggcg cggacgacag
      541 ctttgaccag gcggcctatg agcggctggc gaacggcgcg gaccggggcg attggcggcg
      601 ggaaaacgtc aacgccctgg tgcgggacgc ctatgcggcg gtaaaagcgg tcaacccgtc
      661 cctcacgttc ggcatcagcc cgcaggggaa taacgacaac aattacaaca tccagtatag
      721 cgacgtggct ctctggatgg cggaaagcgg ctatgtggat tatgtgatgc cgcagatcta
      781 ttgggggttc gactaccgca ccggcagcgg gcgggacgat tttgcctttg aaaactgcct
      841 tgccgggtgg ctggccctgc cgcgggcgca gggggtcaag ctctacgtgg ggctgggggc
      901 ctaccgcatc ggcgccgggg acgggggcag caacgaccag gcggaatggt ccagcggctc
      961 caatctggcc cggcaggtga gggcgctgcg gcaggcgggc gcggatggct atgggctgta
     1021 cagctatcgc tggctgttcc agaacgatca gcccaccgtg gcggccgagg tggaagcgct
     1081 caccggcgcg aacgggtgag cgcagggcct gaaacggcct taccggaaca acaaaaagtg
     1141 taattgagac ataaaataat acaaaaagga taaacgcggg gcggagttcg ccccgcgttt
     1201 tcggagggga cgaccggatg aggatgagac acaaggccta cgccagaccg gagctggagg
     1261 cctgcccgtt ttttatcgga gacccggagc ggtggaaagg gcgctggcag aaagcgtttg
     1321 accagccccg gcccctggtg ctggagcttg gctgcggcaa gggggcctat gcggcggcga
     1381 tggcggccgc ccacccggag atcaactatc tggcggtgga ttatctcagc gacgtgctgg
     1441 tgctggccaa gcgcagggtg gaagaggcct atgccgccgc agggcgaccg gtggacaatt
     1501 gccgcctgac ggcgctgaac atccagtgga tcgacgccga cctggggccg gaggatgtgg
     1561 tggagcgcat ccacatcaat ttttgtaacc cctggcccaa gtttggccac aaaaaacggc
     1621 ggctcaccca tccccgccag ctgcagcact accgcgcctt tctgcgggac ggcggcgagg
     1681 tgtggttcaa gaccgacgac gacgggctgt accgggagag cctggagtat tttgcccagt
     1741 gcgggttttc gctgcgctac caaacccttg acctgcacgc gcaggagccg tcgtggaacc
     1801 tgcgcaccga gtatgaggag cggttttccg cgcagggcgt gcccatcaag gccctgatcg
     1861 cggtgaaaga gccgtgaatc cgttggcgcc gttccgccgg gggacagccg cctgcgcgcc
     1921 acgggccgct gggcggcttg ctcttgtcct ccgctgcggc gcgacgctta aactgtccgc
     1981 tatttcccca aaaatatccc tgccccggag aacggcgcct tttcgcgata ttgagccttc
     2041 ccgcaaaaag attcgccgcc cccgcaccgg ttggctggga aattccctaa aggagcgcgg
     2101 gcggagggcc gccggtccct gcgtagggga aaaagggccg ctcggcgcgg gagcggagcg
     2161 gggaggagga cgaaaatgtt ggaagtgaag ttttatgagg agggggaagc gctgggcccg
     2221 aagctgcgct ttgccgtcgt cgccgcgctc tgcggcgggc gctgggtcct ttgcaaacac
     2281 aaaacccgcg ccacctggga gtttccgggc ggccaccgcg agccgggcga gccgatcgcg
     2341 gagacggcgc ggcgggagct gtatgaggag accggcgcgc tggactatcg gctgcgcccg
     2401 gtctgtgcct atggggtgcg ggacccgcag ggcgcggggg aggaaacctt cggcatgctc
     2461 tatctggccg aaatcagccg ttttggcccc ctgccggcgg agtttgagat ggagcggatc
     2521 cagctttttg accggccgcc gcaggcgccg gcccagtgga cctacccgga gatacagccc
     2581 ccgctgctgg aacggttggg gcgggagctg gcgcgggaat gacagagagg gaaacggttt
     2641 tcaccgtttc cctctttcgc tgtggggcgg ccgccgcgcc ccgggcggcg cttgcggcgg
     2701 gatgcggcgt cggagcgtcg attctttcgc cccgcccggc agagcgcccg tggcgctcag
     2761 cagcagcgct ccgctttctt gcaggcaagc cttttccccg aacatccgat gccgcatggt
     2821 tttgtcccat aggacaagac cggcatcccc tgccgtcaac gcgttttgtc ggcaacgaag
     2881 taaatgaggg aatgaagcac ctaacattat tccgagcaat acggataagg ctgtgatcga
     2941 ggttcagagt gatgatccct agaattgatt ttgaggaaag aatccttttg aacaaaatag
     3001 ggctgtggat caaccaaggg ccgcagaaaa cgcaggcaag cctttgtggc ttaacgagta
     3061 tttttgtgag atgtgcagaa taaagcgtct atgttttcaa ggggtgattt tgtcgacagc
     3121 cccaaactgt ccacaagtgc gatggggcaa aagcccgaaa aaaggctgcg caaggaacgc
     3181 gcggcacaga gctttgatga ttcttgaggc cgagcggcca ggatgctata ctgaatttac
     3241 ccatcgccat ttatgaaaaa agggcaagtg agggatttga ggcaggggat tttaagagag
     3301 gaatccgaga atatggatag aaaaatactg gtcgaccaaa gcattgactt tattatgcag
     3361 catttgaatg aacctctgtc gcttgatact gtggcagatc aggttcactt gtcaaagtac
     3421 cattttagcc gaatcttcaa agaagaaacc ggcgagagcg tctatgcgtt tatcaaacgc
     3481 tgtaaggtcg atcaaagtgc aatcgacatg aaactaaacc caacaaaacc aatcatagaa
     3541 attggtttgg attacggata cagcgcttcc aattacagtt ctttatttag aaagcaccat
     3601 gatacttccc ccacgatgtt taggcagtca atcccaactg atggtatggc ggttcccttt
     3661 gcacctg
//
LOCUS       DBMDYA010000224         3666 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5812_length_3666_cov_1.342496, whole genome shotgun sequence.
ACCESSION   DBMDYA010000224 DBMDYA010000000
VERSION     DBMDYA010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_3666_cov_1.342496"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="ACZPFK_06545"
     CDS             complement(<1..515)
                     /locus_tag="ACZPFK_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1406004.1"
                     /translation="MKRTKKIGAALAGLLVLAVLALGSACVVTQQNEYTVVRQFGEVV
                     DIRSTAGVSLKIPLIQTASTLPNTVLLYDLPISDVITQDKKTMVADSFVLWKIEDPKL
                     FIQTLNGSISNAESRLSTIVYNSMKNIISSRTQSDVISGRDGELAADIRDGVGDTLTQ
                     YGIELIAVETK"
     gene            complement(515..1558)
                     /gene="hflK"
                     /locus_tag="ACZPFK_06550"
     CDS             complement(515..1558)
                     /gene="hflK"
                     /locus_tag="ACZPFK_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR1406005.1"
                     /translation="MQMRDVGGWFGRKAPGADAPGGGDGNRPREGQKGLFQRFAGRVG
                     LVLAAAALVVAGLNAFYMINEQENAVVVTFGVPSPVTASGLHFKVPFVQQVRKVDMTI
                     HGFAIGYDSATKEPIPDESLMITSDYNFVNVDFFVEYRVTDPVKYLYASQDPEFILKT
                     LAQSYIRDTIGIYPVDDVITTGKNQIQGEIKDKIMARLEKEDIGLQLVNITIQDAEPP
                     TAEVLTAFKEVETAKQSADTAVNNANKYRSEQIPAAEAQADQILQNAQAQKEARINEA
                     NGQVARFNSMYQEYARYPLITKQRMYYETMEEVLPGLRVIIQDGSGSTLNILDGDGAL
                     KTQPVQPAEEEEK"
     gene            complement(2785..>3666)
                     /locus_tag="ACZPFK_06555"
     CDS             complement(2785..>3666)
                     /locus_tag="ACZPFK_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1406006.1"
                     /translation="GMRKNYVRVIAATLLFGSIGLFVRAIPLASSVIALVRAVIGALV
                     LLLAALAGRQKLDGAAMRRNLPLLALSGAFIGFNWILLFEAYRYTTVGIATLAYYAAP
                     VLVVLLAPLILRERFTAPRLAGVLAAAAGTLLLTGNGGGAGSEPLKGILLGLGAAVLY
                     AGVILTNKFITGLSGLERTFTQLVVAATVLLPYVLATRPAAPGPMTGAGLACLLAVGV
                     LHTGFGYYLYFSAMGELPGQTIALLGYIDPVSALLFSALFLGERLSPLQLAGAVLILG
                     GAAFGELYKGRQKQMEE"
BASE COUNT          568 a         1291 c         1129 g          678 t
ORIGIN      
        1 tgcttggtct ccaccgcgat cagctcgatg ccgtactggg tcagggtgtc ccccaccccg
       61 tcgcggatat ccgcggccag ctcgccgtcc cgcccgctga tgacgtcgct ctgggtgcgc
      121 gagctgatga tgtttttcat gctgttgtag acgatggtgg aaaggcggct ctcggcgttg
      181 gagatgctgc cgttcagggt ctggatgaag agcttggggt cctcgatctt ccacagcaca
      241 aagctgtcgg ccaccatggt ctttttgtcc tgggtgatca cgtccgagat gggcaggtcg
      301 tacagcagca ccgtgttggg cagggtgctg gcggtctgga tgagggggat cttcaggctg
      361 acgcccgccg tgctgcggat gtccaccacc tcgccgaact gccgcaccac ggtgtactcg
      421 ttctgctggg tcaccacgca ggcgctgccc agcgccagca ccgccagcac cagcagcccg
      481 gccagggcgg ccccgatctt tttcgtccgc ttcatcactt ctcctcctcc tctgccggct
      541 ggacgggctg ggtcttgagg gccccgtcgc cgtccaggat gttcagggtg ctgccgctgc
      601 cgtcctggat gatgacccgc agccccggca gcacctcctc catcgtctca tagtacatcc
      661 gctgcttggt gatcagcggg tagcgggcgt actcctggta catggagttg aagcgggcca
      721 cctggccgtt ggcctcgttg atccgggcct ccttttgggc ctgggcgttc tgcaggatct
      781 ggtcggcctg ggcctccgcc gccgggatct gctccgagcg gtacttgttg gcgttgttca
      841 ccgcggtgtc ggcgctctgc tttgcggtct ccacctcttt aaaggcggtc agcacctcgg
      901 cggtgggcgg ctcggcgtcc tggatggtga tgttcaccag ctgcaggccg atgtcctcct
      961 tttccagccg ggccatgatc ttgtccttga tctcgccctg gatctggttt ttgccggtgg
     1021 tgatgacgtc gtccaccggg tagatgccga tggtgtcccg gatgtagctc tgggccaggg
     1081 tcttgaggat gaactcgggg tcctggctgg catagagata cttcaccggg tcggtcaccc
     1141 ggtactcgac gaagaaatcc acattgacga agttgtagtc cgaggtgatc atcaggctct
     1201 cgtcggggat gggctccttg gtggccgaat catacccgat ggcaaagccg tggatggtca
     1261 tgtccacctt gcgcacctgc tgcacaaagg gcactttaaa atgcaggccg ctggcggtga
     1321 ccgggctggg caccccaaag gtgaccacca ccgcgttctc ctgctcgttg atcatgtaaa
     1381 aggcgttcag gcccgcgacc accagcgccg cggccgccag caccaggccg acgcggccgg
     1441 caaagcgctg gaacaggcct ttctgcccct cgcgcgggcg gttgccgtcc ccgccgccgg
     1501 gggcgtccgc cccgggggcc ttgcggccga accagccgcc gacatctctc atctgcatcg
     1561 ctctctcctt atcttttcct gtctggccgg gccccgcttc gaagacgggg cgccgggcgg
     1621 tgggtgaggg gctcgagcct ctccgcttga tgaatagttt aaaggatttg ggccgcaaag
     1681 gcaaagacgg aattgttatt tttttgacat tccccgttcc gccccgcccc ctgggggcgg
     1741 agcagaattt ccgctcgccg ccgcccgccc tctctctcgc tgtatctcgt gttttctccc
     1801 tttttgccga aagagaggtg aaaacgccgg gccgcgcccg ctgcccgcag gcaaaagggc
     1861 ggacgctgcc tctctccgcg ccgcctaggt tttccgcgcc gtctgcgggg gcagcgtctc
     1921 cgcgcggctc atttgccgct ctcccggccc ctgccgccgc ccacctccgg cccccgcaaa
     1981 gcgggaaccg tttctccctg ccggggggga ggtagaaagc gcgcgctttg aggcctgccg
     2041 cgggccccgg cgcacagtgc ttttctcccg cccgcaccgc ccgcagcggc cactccagag
     2101 ctccccggcg aggggaaccg gccgcccaag gcggtctggg gggcaatttt caagaggcaa
     2161 attagattct ccaacccggc actatttttt ccattttgaa aatggaaacc ctcaatgccc
     2221 tttggggctc gctccacttc gttccgcccg ccgcttccga gcccctccaa gcgccctcaa
     2281 aatcgccctg aaaggtctct aaaagcttct tcaccgccct agtttgaaac gcgcagaaac
     2341 ggcggtttat agccatcccc gaggggttgc aggtggggtg catgtgtcca gttgcgccca
     2401 gaagctttcc gtttctcgtt ttgcctcaaa atcccccttg ttggagggct tgagatggag
     2461 cgagaagcaa aaagagcccc ggcatctttc gatgcctggg gctccttttt tagaaaatcc
     2521 aatttgcccc accttagcgg tgccgccggg cgctttcgcc gcgccccgcc ctcagcggcc
     2581 acttcaaagc cctccccggc aaagggaacc ggccgcccaa gggggccgcc ccgccgctcc
     2641 cccccagccc cggcccgcgt ccgcgcttcc ctctgggcgc ttcccgcctc agaagcccct
     2701 tccccggccc gccccacaaa aaaagccccg gtctgcaccg gggccgcgcg ctccggggcg
     2761 gcccgccgcc gcccctcgcc gccgctattc ctccatctgc ttttgccgtc ccttgtacag
     2821 ctcgccgaac gccgcgccgc ccagaatcag caccgcgccc gccagctgca gcggcgagag
     2881 ccgctcgccg agaaacagcg ccgaaaagag cagcgcgctc accgggtcga tgtagcccag
     2941 cagggcgatg gtctggccgg gcagctcgcc catggccgaa aagtacagat aatacccgaa
     3001 gccggtgtgc agcaccccca ccgccagcag gcaggcgagg cccgcgccgg tcatcggccc
     3061 cggggccgcg ggccgggtgg ccagcacata gggcagcagc accgtggccg ccaccaccag
     3121 ctgggtgaac gtgcgctcca gccccgagag gccggtgatg aacttgttgg tcaggatgac
     3181 cccggcatag agcaccgccg cgccgaggcc cagcaaaatc cctttcagcg gctcgctgcc
     3241 cgcgccgccc ccgttgccgg tgagcagcag cgtgcccgcc gccgcggcca gcacccccgc
     3301 cagcctgggg gcggtgaacc gctcgcgcag gatcagcggg gccagcagca ccaccagcac
     3361 cggggccgcg taataggcca aagtggcgat gcccaccgtg gtatagcggt aggcctcgaa
     3421 cagcaaaatc cagttgaagc cgatgaacgc gccggacagc gccagcagcg gcaggttgcg
     3481 ccgcatggcc gcgccgtcca gcttttgccg ccccgccagg gctgccagca gcagcaccag
     3541 cgcgccgatc accgcccgca ccagcgcgat gacgctgctg gcgagcggga tggcccgcac
     3601 gaacaggccg atgctgccga acagcagcgt cgccgcgatc acccttacat agtttttcct
     3661 catacc
//
LOCUS       DBMDYA010000225         3666 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5814_length_3666_cov_1.050771, whole genome shotgun sequence.
ACCESSION   DBMDYA010000225 DBMDYA010000000
VERSION     DBMDYA010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5814_length_3666_cov_1.050771"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(12..956)
                     /locus_tag="ACZPFK_06560"
     CDS             complement(12..956)
                     /locus_tag="ACZPFK_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014594.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR1406007.1"
                     /translation="MDWQQRYRQKLATPQQAVRRIRSHSRIAVGHAAGEPLSLIEAMI
                     QGRGRYRDLEILQMETPGPAVYARPGMEAHFRHNAVFVGEGTRDIVMAGKGDFTPIHF
                     SQLAALFGGPLPIDVALIQVSPPDEHGYCSLGPTVDYIKEAAESAGLVLAEVNDRMPR
                     TLGDSFLHMGRIDCAVPVSRPLPGLTRERAGPQELEAGRRCARLIRDGDTLQAGLGAA
                     CDGVLCALGQKRDLGLHSELFTEAMLDLVEQGAVNGSRKTLHPGKIVAAALAGGERLY
                     RFADNNPSLELYPASYVDAPGVILRNRNMVSITSCMEA"
     gene            complement(991..2103)
                     /locus_tag="ACZPFK_06565"
     CDS             complement(991..2103)
                     /locus_tag="ACZPFK_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HIR1406008.1"
                     /translation="MKQFELHPALYRYDSCEQFCRALEIGPGDLILTNEFLYRPFFGG
                     RELGAQVIFQEKYGGGEPSDEMAEAIWRDVQGDPRRVIGIGGGTVLDLSKLFALETVT
                     PILDLYDKKTEPRKKRRLVLVPTTCGTGSEVTDISILELKSRSTKLGLAHEALYADEA
                     ALVPQLLSTLPYRFFATSSIDALIHAVESSVSPKATEYSKLFGHQAIRMILAGYRRIQ
                     REGRQVYGELLGDFLTASNFAGCAFSNAGCGAVHAMSYPLSGKYHVAHGEANYALFTG
                     IFRNYMEISAEGRIDELNGLLAAQLDCPKSEVYEKLEELLSAVLTRKPLREYGVTEAD
                     LDDFTANVMEKQGRITANNFVPLDAVRVRKIYGELY"
     gene            complement(2198..2470)
                     /locus_tag="ACZPFK_06570"
     CDS             complement(2198..2470)
                     /locus_tag="ACZPFK_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406009.1"
                     /translation="MEIAKMQQVAFLHHEKRTRKAAKCFYFLSENRISRIKRRPPVAK
                     LQRVGAFLRSGGAARYKNGAPYAKEEKVNWQCLQFLEYRIVKTHKK"
     gene            complement(2537..3484)
                     /locus_tag="ACZPFK_06575"
     CDS             complement(2537..3484)
                     /locus_tag="ACZPFK_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR1406010.1"
                     /translation="MGNEDLIVADRNMQNVLRMVSRVARLDTTVLLLGETGVGKEEVA
                     KHLYRTSSRSDKQFIKVNCGAIPAELIESELFGYEKGAFTGANREGKVGLFEVADRGT
                     IFLDEVGELPLEMQVKLLRVLQEQEIERIGSSRTIKIDVRVLAATNRDLEEMVARKLF
                     REDLYYRLNVFPITIPPLRERPDDIVPFARSFLAELSRKYGMKKEFLPSALASLRGYD
                     WPGNVRELKNIVERAAILSNGPTICAEDLSLRPAASVAEPAERRALGETVDLKRLVEE
                     FELEYIEAAYAEYGNVREAAASLGMDASTFVRKRKRYRR"
     gene            complement(3484..>3666)
                     /locus_tag="ACZPFK_06580"
     CDS             complement(3484..>3666)
                     /locus_tag="ACZPFK_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406011.1"
                     /translation="RNGNRAMITSSPILAENGRVAMVVTNVRDITEICRLQEELERNE
                     EKSGAPPARRRRCGGS"
BASE COUNT          588 a         1242 c         1093 g          743 t
ORIGIN      
        1 catccggatg actacgcctc catgcaggag gtgatcgaca ccatattgcg gttgcgcagg
       61 atcacgccgg gcgcgtccac atagctggcg gggtacagct caaggcttgg gttgttgtcc
      121 gcaaagcggt agagccgctc gcccccggcc agggcggccg ccacgatctt gcccgggtgc
      181 agcgtcttgc ggctgccgtt gacggccccc tgctccacca ggtccagcat ggcctcggta
      241 aagagctcgc tgtgcaggcc gaggtcccgc ttttggccca gcgcgcagag cacgccgtcg
      301 cacgccgccc ccaggcccgc ctgcagggtg tcgccgtcgc ggatgagcct cgcgcaccgg
      361 cggcccgcct ccagctcctg cgggcccgcc cgctcccttg tgaggcccgg cagcgggcgg
      421 ctgaccggca cggcgcagtc gatgcgcccc atgtgcagaa agctgtcgcc cagggtgcgg
      481 ggcatgcggt cgttcacctc cgccagcacc aggccggcgc tctccgccgc ctcttttatg
      541 taatccaccg tgggccccag gctgcagtag ccgtgttcat ccggcggcga tacctggatc
      601 agcgccacat cgatgggcag cgggccgccg aacagggccg cgagctgcga gaagtggatg
      661 ggggtgaaat cccccttgcc cgccatgacg atatcccgag tgccctcgcc cacaaagacg
      721 gcgttgtgcc gaaaatgtgc ctccatgccc ggcctggcgt agaccgccgg ccccggcgtc
      781 tccatctgca gaatctccaa atcccggtag cggccccgcc cctggatcat cgcctcgatc
      841 agcgacagcg gctcgcccgc cgcgtggccg acggcgatgc ggctgtggga tcggatgcgg
      901 cgcacggcct gctgcggggt ggcgagcttt tggcgatagc gctgctgcca gtccataccg
      961 gcctcctttc cgccctgcgc tgcgccggcc tcagtagagc tcgccgtaga tcttgcgcac
     1021 ccggacagcg tccagcggca cgaagttgtt ggccgtgatg cggccctgtt tttccatcac
     1081 gtttgcggtg aagtcgtcca ggtccgcctc ggtgaccccg tactcgcgca ggggcttgcg
     1141 ggtgagcacg gcggagagca gctcttccag cttttcgtac acctcgcttt tggggcaatc
     1201 cagctgcgcg gccaacaggc cgttcagctc gtcgatgcgc ccctctgcgc tgatctccat
     1261 ataattgcgg aaaatgccgg tgaacagggc gtagttcgcc tccccgtggg ccacatggta
     1321 tttgccgctg agtggatagc tcatggcgtg gaccgccccg cagcccgcgt tgctgaacgc
     1381 gcagcccgca aagttgctgg cggtgagaaa atcgcccagc agctcgccgt acacctgccg
     1441 gccctcccgc tggatgcgtc gatagcccgc cagaatcatg cggatggcct ggtggccgaa
     1501 cagtttgctg tactcggtgg ctttggggga aacgctggat tccaccgcgt ggatgagggc
     1561 gtcgatggag ctggtggcga aaaaccggta gggcagcgtg ctgagcagct gcggcaccag
     1621 cgccgcctcg tccgcataaa gcgcctcatg ggccagcccc agcttggtgc tgcggctctt
     1681 gagctccaaa atcgagatgt cggtcacctc gctgccggtg ccgcaggtgg tgggcaccag
     1741 caccaggcgg cgctttttgc gcggctcggt ctttttgtcg tagaggtcca ggatgggggt
     1801 gaccgtctcg agggcgaaca gcttggaaag gtccaaaacc gtccctcccc cgatgccgat
     1861 gacccgccgc gggtccccct gcacatcccg ccagattgcc tccgccatct cgtcggacgg
     1921 ctcgccgccg ccgtactttt cctgaaaaat cacctgagcc cccagctcgc gcccgccgaa
     1981 gaacggacgg taaaggaact cgttggtgag gatcaggtca cccgggccga tctcaagcgc
     2041 gcggcagaac tgttcgcagc tgtcgtagcg gtaaagcgcc ggatgcaatt caaactgttt
     2101 cataccggtc tcctttctct tccatcgtcc aatctgcttt cacccctcta caaggaagca
     2161 tattccgtgc cagctttgat tatttttgct atatttttta ttttttgtgt gttttaacta
     2221 ttctatattc caaaaattgt aggcattgcc aattgacctt ttcttctttt gcgtatggag
     2281 ctccgttttt gtaccgggcc gcgccgccgg accgcaaaaa agcgcccacc cgttgcaatt
     2341 tcgcaacagg tgggcgcctt tttattcttg aaattcgatt ttcagacaaa aagtaaaaac
     2401 attttgcagc ttttcgtgtt cttttttcgt gatgcaaaaa cgcaacttgt tgcatttttg
     2461 caatttccac gtctctgcgg gcactccctc agggcgggcc ggccgccaca gccgcccggc
     2521 ggctctcgcc gttttttcat cgccggtagc gcttgcgctt gcgcacaaag gtggaggcgt
     2581 ccatgcccag gctggccgcc gcctcccgca cattgccgta ctccgcgtaa gccgcctcga
     2641 tatactccag ctcaaactct tccaccaggc gcttcaggtc caccgtctcg cccagggcgc
     2701 gccgttccgc aggctcggcc accgaggcgg cggggcgcag ggacaggtcc tcggcgcaga
     2761 tggtgggccc gttgctgagg atggcggccc gctcgacgat atttttcagc tcccgcacgt
     2821 ttccgggcca gtcgtacccc cgcaggctgg cgagggccga gggcaaaaac tcctttttca
     2881 tgccgtattt gcggctcagc tcggccaaaa agcttctggc aaaggggacg atatcatccg
     2941 gccgctcccg cagcggcggg atcgtgatgg gaaagacgtt gagccggtag tagaggtcct
     3001 cgcgaaacag cttgcgggcc accatctcct ccaggtcgcg gttggtggcc gccagcaccc
     3061 gcacgtcgat cttgatggtg cggctcgagc cgatgcgctc gatctcctgc tcctgcagca
     3121 cccgcagcag cttgacctgc atctccagcg gcagctcgcc cacctcgtcc aaaaagatgg
     3181 tgccccggtc cgccacctcg aacaagccca ccttgccctc ccggttggcg ccggtaaaag
     3241 cgcctttttc atagccgaac agctcgctct cgatcagctc ggccgggatg gccccgcagt
     3301 tcaccttgat gaactgcttg tcgctgcggc tgctggtgcg gtagagatgc ttcgccacct
     3361 cctccttgcc cacacccgtc tcgcccagca gcagcacggt ggtgtccagc cgcgccacgc
     3421 ggctcaccat gcgcagcacg ttctgcatgt tgcggtcggc cacgatcagg tcctcattgc
     3481 ccatcagctg ccgccgcagc gcctccgcct cgctggtgga gcgccgcttt tttcctcgtt
     3541 ccgctccagc tcctcctgca ggcggcagat ctcggtgatg tcccgcacat tggtgaccac
     3601 catcgccacg cggccgttct ccgccaggat cggcgaactt gtaatcatgg cccggttccc
     3661 gttgcg
//
LOCUS       DBMDYA010000226         3664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5819_length_3664_cov_1.448779, whole genome shotgun sequence.
ACCESSION   DBMDYA010000226 DBMDYA010000000
VERSION     DBMDYA010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5819_length_3664_cov_1.448779"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="ACZPFK_06585"
     CDS             <1..250
                     /locus_tag="ACZPFK_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406012.1"
                     /translation="GLNAQFDRLSGRDSEPAYGMGRLDKTDFEALLAGLKTPMLADAT
                     LKEKIGAGAEAYCRGELTLEEALGQIKSETALYLAERG"
     gene            complement(229..1575)
                     /locus_tag="ACZPFK_06590"
     CDS             complement(229..1575)
                     /locus_tag="ACZPFK_06590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1406013.1"
                     /translation="MIKKLRRRLTLHYILLSGLVLLATCVSGFLLSARQRQVSDELLF
                     QTHLSSLTGRLQFDRRIGGDWLASFEAENRLAVFLWDSGRRMKFEGGVWLPSARRGAL
                     AAAALEEAAAEGFYPADPPASYTASGFELERDGLRFRGAAAAFPLEGGSWIHLLLLRD
                     LAPQRAALGALALRWTLLFLAAGTALAFAGRWLAGRAAAPVQDSLRRQAEFVAAASHE
                     LRGPLAVVATSADLLAEGMEGPYLANIRGEARRMAGLVDDLLTLAGAEAGLAPPAVPV
                     EPDTLLIEAYDRFAPVCAGRGLRLRAELPQAALPPVAGDPERLMQVICALLSNACAYT
                     PPGGTITLCGKVRRGQVALQVADTGPGVPEALREKIFERFYRADPSRTDKAHYGLGLP
                     VARRLAALYGGRLTAGEAAGGGALFTLSLPILSAPAPHRRGEKPNPGGRPHPRSAR"
     gene            complement(1572..2252)
                     /locus_tag="ACZPFK_06595"
     CDS             complement(1572..2252)
                     /locus_tag="ACZPFK_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1406014.1"
                     /translation="MRILLIEDDAALREAVAAKLRAEGFEVTGCADGDEGGWQLGQDA
                     WDLVLLDRMLPGRDGLALLRGLRRSGSQTPVLLLTARGAVSDRVEGLDAGADDYLTKP
                     FDLRELSARVRALIRRPVLARRDPPEAGGLSLDEAGCVLTGPGGSCTLSRRECALLSL
                     LLQNAGRTLSRETLLARVWGPDAEVESTVLDTYIHFVRRRLAAVGARAEIRNRRGVGY
                     LLCAEDAP"
     gene            2456..>3664
                     /locus_tag="ACZPFK_06600"
     CDS             2456..>3664
                     /locus_tag="ACZPFK_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406015.1"
                     /translation="MKRFLSLLLCCALALCAAGCGEAPAPAQATGRYVEHEITPKGLS
                     AGEARGLFQLADGALCYLGGMLDFFISIDGGDSWQRQDTGWYEALAQMAGTQAITLGV
                     TREGAVWAAAPSEDGFALFCVEDGAPRRTADVAAGEELHPVQLQWDGEGRLFVMAFPD
                     EGDALLLQVDADGGVERRFAVDYGVNFEVADGRAELFTYDGEQITYDLAAGRETARRT
                     LSGEAVGALAPDGAAWAVSADGIFCVTAGGSVAEALFAGTGNRFQAPGYSPRAVLPLQ
                     NGSLCVAYDSARGAAVCRYDYDPDALVRGENALVVWALHGGLTLNAAVTAFKEAHPEV
                     DVVLELGHTSEDDGLDDAEIIRTLNTALLAGDAPDLLILDGLPADSLIRKGMLEDLSG
                     LVDGTEYFENL"
BASE COUNT          582 a         1258 c         1304 g          520 t
ORIGIN      
        1 ggggctgaac gcccagttcg accgcctctc cggcagggat agtgagcccg catacggcat
       61 ggggcggctg gacaagacgg attttgaggc gctgcttgcc gggctgaaaa cccccatgct
      121 ggccgacgcg accctcaagg agaagatcgg cgccggggcc gaggcctact gccggggcga
      181 gctgaccctg gaggaggcgc tgggccagat caagagcgag accgcgcttt atctggctga
      241 gcgcggatga ggccggccgc cggggttcgg tttttctccc cgccggtgtg gggcgggggc
      301 ggacaggatg ggcagggaca gggtgaacag cgccccgccg cccgccgcct cccctgcggt
      361 gagccgcccg ccgtagaggg cggccagccg ccgggccacc ggcaggccca gcccatagtg
      421 ggccttgtcg gtgcgggagg ggtcggcgcg gtaaaagcgc tcgaagattt tttcccgcag
      481 cgcctcgggc acgccgggcc cggtgtccgc cacctgcagc gccacctgcc cgcgccgcac
      541 tttgccgcaa agggtgatcg tcccgcccgg cggggtgtaa gcacaggcgt tggagagcag
      601 ggcgcagatc acctgcatca gccgttccgg gtccccggcc accgggggca gggcggcctg
      661 cggcagctct gcgcgcagcc gcaggccgcg ccccgcacag acgggggcga agcggtcgta
      721 ggcctcgatc agcagggtgt cgggctccac cggcacagcg ggtggggcga ggcccgcctc
      781 ggcccccgcc agggtgagaa gatcgtcgac gaggcctgcc atgcggcggg cctcgccgcg
      841 gatattggca aggtacggcc cctccatccc ctcggccagc aggtcggcgc tggtggcgac
      901 gacggcgagc gggccccgca gctcatggct tgcggcggcg acgaattccg cctgccggcg
      961 caggctgtcc tgcaccggcg cggcggcccg tccggccagc cagcgcccgg caaaagccag
     1021 cgccgtcccc gcggcgagaa aaagcagcgt ccagcgcagg gccaacgccc ccagggcggc
     1081 gcgctgtggg gcgaggtcac gcagcaaaag caggtggatc cagctgccgc cctccagcgg
     1141 aaacgccgcc gccgcaccgc gaaagcgcag gccgtccctc tccagctcaa agccggaggc
     1201 ggtatagctt gcgggcgggt ccgccgggta aaagccctcc gctgcggcct cttccagcgc
     1261 cgcggcggcg agcgcgccgc gccgggccga cggcagccac accccgccct cgaatttcat
     1321 ccgccggccc gagtcccaca gaaaaaccgc caggcggttt tccgcctcaa aagaggcgag
     1381 ccagtcgccg ccgatgcgcc ggtcgaattg caggcgtccg gtcaggctgg acagatgggt
     1441 ctgaaagaga agctcgtcgc tgacctgccg ctgccgcgcc gagagaagaa agccggacac
     1501 acaggtcgcc agcagcacca ggcccgatag gaggatgtag tgcagcgtca ggcggcggcg
     1561 caattttttg atcatggcgc atcctccgcg cacagcagat agcccacgcc gcgccggttg
     1621 cggatctccg cccgcgcccc cacggcggcg agacggcgac gcacaaagtg gatataggtg
     1681 tccagcaccg tgctctccac ctcggcgtcc gggccccaca cccgcgccag cagggtctcg
     1741 cgggacaagg tgcgccccgc gttttgcagc agcagcgaga gcagcgcgca ctcgcgccgc
     1801 gacagggtgc agctcccgcc ggggccggtg agtacgcacc cggcctcgtc cagcgagagg
     1861 ccgcctgcct cgggcgggtc ccgccgcgcc agaacggggc gccggatcag ggcccgcacc
     1921 cgggcggaga gctcgcgcag gtcaaagggc ttggtcagat agtcgtccgc gcccgcgtcc
     1981 aacccctcca cccggtcgct caccgcgccc cgcgcggtga gcagcagtac cggcgtctgg
     2041 ctgccgctgc ggcgcagccc gcgcagcagg gcaaggccgt cgcgcccggg cagcatgcgg
     2101 tccagcagca ccaggtccca ggcgtcctgg cccagctgcc agccgccctc gtccccgtcg
     2161 gcgcagccgg tcacctcaaa gccctcggcg cgcagcttgg cggccaccgc ctcccgcagg
     2221 gccgcgtcgt cctcgatcag caagatacgc attggcatgg ctccttttcg cagtttcagt
     2281 cgttcctttc aaaaagacgg tcgcctttct acccagggga cgcgccagct ccctttaagg
     2341 taacagaaaa gtcttaactt ttccttaaac ctgccgcgaa cggcgcggat taaagcttaa
     2401 tttaagaaac ggctggtagc atgcagatat cccaaaaaat gaggagcgat gcgcgatgaa
     2461 acgttttcta tcccttttgc tctgctgtgc gctggcgctt tgcgccgcgg gctgcggcga
     2521 ggcgcccgcc ccggcgcagg ccaccgggcg ctacgtggag cacgagatca ccccgaaggg
     2581 gctttccgca ggcgaggcca ggggcctgtt ccagctggcg gacggcgcct tgtgctatct
     2641 gggcggcatg ctggacttct ttatcagcat cgacggcggc gacagctggc agcggcagga
     2701 caccggctgg tacgaggcgc ttgcccagat ggcgggcacc caggcgatca cgctgggggt
     2761 aacgcgggag ggcgcggtct gggccgcggc cccgtcggag gacggctttg ccctgttttg
     2821 tgtggaagac ggcgccccgc gccgaacggc cgacgtcgct gccggggagg aactgcaccc
     2881 cgtgcagctt caatgggacg gggagggcag gctgtttgtg atggcgtttc ccgacgaggg
     2941 ggacgccctg ctgctgcagg tcgatgccga cggcggggtg gaacggcgct ttgccgtgga
     3001 ctatggcgtc aattttgagg tggcggacgg cagggcggag ctgttcacct acgatggaga
     3061 gcagatcacc tacgatctcg ccgccgggcg cgagaccgcc cggcgcaccc tgagcgggga
     3121 ggcggtcggc gccttggccc cggacggtgc ggcctgggcc gtttccgcag acggcatctt
     3181 ctgcgtcacg gcgggcggca gtgtggccga agctcttttt gcgggcaccg gcaaccgctt
     3241 tcaggcaccc ggttactccc cgcgcgcggt gcttccgctg caaaacggca gcctctgcgt
     3301 ggcctacgac agcgcccggg gcgcggcggt ctgccgctac gattatgacc cggacgctct
     3361 ggtgcggggc gagaatgcgc tggtcgtgtg ggccctgcac ggcggcttga ccctgaacgc
     3421 cgccgtcacc gcctttaaag aggcacaccc cgaggtggat gtggtgctgg agctggggca
     3481 cacctctgag gacgacggcc tggacgacgc ggagatcatt cgcaccctca ataccgctct
     3541 gctggcgggg gatgccccgg acctgctgat cctggacggc ctgcccgccg acagcctgat
     3601 ccgaaaaggc atgctggagg acctgagcgg cctggtggac gggacagagt attttgaaaa
     3661 cctg
//
LOCUS       DBMDYA010000227         3655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5840_length_3655_cov_1.174451, whole genome shotgun sequence.
ACCESSION   DBMDYA010000227 DBMDYA010000000
VERSION     DBMDYA010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5840_length_3655_cov_1.174451"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="ACZPFK_06605"
     CDS             <1..196
                     /locus_tag="ACZPFK_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406016.1"
                     /translation="GRVLFVGDAAYEELVGNDWVDHRDRLELFRRELEQMDFTHCFVG
                     HGAPMPREQLFAWFGERLGS"
     gene            complement(437..1237)
                     /locus_tag="ACZPFK_06610"
     CDS             complement(437..1237)
                     /locus_tag="ACZPFK_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406017.1"
                     /translation="MINKTLFAREVRSSYKLLLIFLAVLTLYGSMIVAMFDPKLGDSL
                     NLMAESMPEIFAAFGMLDVGATLTQFIVNYLYGFLLIAFPLVFIVLLSNRLVARYVDR
                     GSMAYLLATPNRRGRLARTQALVLLLSSLCIVLWMTGLCWGVSAALFPGKLEVGAFLS
                     VNAALYALLIFLSGLCFCASCCCNDTRWSCGIGAGAGIAFLLIQMVSQVGEKFEGLKY
                     INPLTLFAPKALLAGESWPVWGAAALAAAGLALIGAGIGAFSRRDLPV"
     gene            complement(1227..2114)
                     /locus_tag="ACZPFK_06615"
     CDS             complement(1227..2114)
                     /locus_tag="ACZPFK_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406018.1"
                     /translation="MIEAERLTKDYGGGRGVFGLSFAVEKGEVMGFLGPNGAGKTTTI
                     RQLMGFIRPDEGSARIGGRDCFAHAAEIERDLGYLPGEPAFMEDMTGEAFLRFLARMK
                     GLAGFDRAQELMRLFELDGSARIKRMSKGTKQKVAIVAAFMGSPALLILDEPTSGLDP
                     LMQNKFIDLILSEKRRGATILMSSHIFEEVERTCDRTVIIREGRIAAVEQLEKLKKTK
                     RKLFLVQFERPQDASAFAVAWGDGAELQGDTVRAAVTGSPDAFLKAISAYPVRDIDLH
                     RETLEELFLHFYGGGRNDQ"
     gene            complement(2120..2737)
                     /locus_tag="ACZPFK_06620"
     CDS             complement(2120..2737)
                     /locus_tag="ACZPFK_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1406019.1"
                     /translation="MNEKFFALPEEKRRRIVNAAMEAFGKNEYKRASTDDIAARAGIS
                     KGLLFHYFGSKKELYFYLYRHVERMVAEQVADEKFAQISDFFELMAHGAEKKLKLVAE
                     NPYLMEFTVRVYSSRDERLAPELDARLQQTLDGAVGYFKNIDFGKFRPGVDPMELLRM
                     LTWMTLGYLEELRRRGLPVDVEKVMRDYRRWSEMFRSMAYKEEYL"
     gene            3008..>3655
                     /locus_tag="ACZPFK_06625"
     CDS             3008..>3655
                     /locus_tag="ACZPFK_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1406020.1"
                     /translation="MNKTLFENGLVITVDDQDTVYRKGYLLVEGDRIAAVGEGDYAGD
                     RAGVTVVDADGKAVLPGIVDAHTHVAGSLFKGLLEDDPNGFYGFALPMEGQLTPEDVY
                     HLSRLGAWECMQAGVTTVNEIYHYVLSVAKAVDEVGLRAVISQNIVDVDIGALRYNDY
                     TRYPAKGEAFVEDNIRLIEEYHGKDNGRITCRFGPHATDTVSMELAKKCVELGKKY"
BASE COUNT          737 a         1179 c         1038 g          701 t
ORIGIN      
        1 ggggcgtgtg ctctttgtgg gcgacgcggc ctacgaagag ctggtgggca acgattgggt
       61 agaccaccgg gatcggctgg agcttttccg gcgggagctg gagcagatgg atttcaccca
      121 ctgctttgtg gggcacggcg cgccgatgcc acgcgagcag ctgtttgcct ggtttgggga
      181 gcggctgggc agttgaccgg cggcattttt gctttgacca aaaagacagg gcagggaacg
      241 gtttttccgt gcgaaaaacc gttccctgcc ctgtcttttt cttgaaaacc gacgccttga
      301 ggcaaaccga gatgagacga gagcgcaccg tttcacaagg ccgtgctgca aaagaggtgg
      361 gctttcttcc ctgtctcatt ctctcatttc cgctctgcga cctagttttt tgctgcagtg
      421 cggggcgcag cgctcctcac accggcaaat cgcgcctgga aaaggccccg atccccgcgc
      481 ctatcaacgc caggccggcc gccgccagcg ccgccgcccc ccagacgggc cagctctccc
      541 ccgccagcag tgctttgggc gcgaacaggg tcagcgggtt gatatacttg aggccctcga
      601 acttctcccc cacctgcgag accatctgga tcagcaaaaa ggcgatgcct gcgcccgcgc
      661 cgataccgca ggaccagcgg gtgtcgttgc agcagcagga ggcgcaaaag cagaggccgg
      721 acaaaaagat cagcagcgcg tagagagcgg cgttgactga caaaaacgcc cccacctcca
      781 gcttgccggg gaaaagggcc gccgacaccc cccagcagag gccggtcatc cagagcacga
      841 tgcacagcga cgacagcagc agcaccagcg cctgggtgcg ggccagcctg ccgcgccggt
      901 tcggggtcgc cagcagatag gccatcgagc cccggtccac atagcgggcc accagccggt
      961 ttgacagcag cacgatgaac accagcggga aagcgatgag caaaaaaccg tacaggtaat
     1021 tgacgatgaa ttgagtgagg gtcgccccca cgtccagcat gccgaaagcg gcaaagattt
     1081 cgggcatgct ctccgccatc aggttcaggc tgtcccccag cttggggtcg aacatggcga
     1141 cgatcatcga gccgtacagg gtgagcacgg ccaaaaaaat gagcagcagc ttataggacg
     1201 accgcacttc tcttgcaaac agcgttttat tgatcattcc gcccaccccc ataaaaatgc
     1261 aggaacagtt cctccagcgt ctcgcggtgc aggtcaatat cccgcaccgg gtaggcgctt
     1321 atcgccttga gaaaagcgtc cgggctgccg gtcaccgccg ctctcaccgt gtccccctgc
     1381 agctctgcgc cgtcccccca ggccacggca aaggccgatg cgtcctgcgg gcgctcaaac
     1441 tgcactaaaa acagcttgcg cttggttttt ttcagctttt ccagctgctc gacggcggcg
     1501 atgcgcccct cgcggatgat gacggtgcgg tcgcaggtgc gctccacctc ctcaaaaata
     1561 tgggaggaca ttaggatcgt cgcgccccgc cgcttttcgc tcaggatcag gtcgataaat
     1621 ttgttctgca tcagcgggtc cagtccgctg gtgggttcgt ccaggatcag cagggccggg
     1681 ctgcccataa aggcggccac aatcgccacc ttttgcttgg tgcccttgga catccgcttg
     1741 atgcgcgccg agccgtccag ctcgaaaagc cgcatcaact cctgcgcgcg gtcaaacccg
     1801 gcaagtcctt tcatcctcgc caaaaaacgc aaaaaggcct cgccggtcat atcctccata
     1861 aaggcgggct ccccgggcag ataccccaga tcccgctcga tctccgccgc atgggcaaag
     1921 cagtcccgtc cgccgatccg cgccgatccc tcatccgggc gaatgaaacc catcagctga
     1981 cggatggtgg tggtcttgcc cgcgccgttg ggccccaaaa agcccatcac ctctcctttt
     2041 tccaccgcaa agctgaggcc gaagaccccg cgcccgccgc cgtaatcctt ggtcagccgt
     2101 tcagcctcga tcatcctgct cacaaatact cctccttata ggccatgctg cggaacatct
     2161 ccgaccagcg ccggtaatcc cgcatcacct tttccacatc caccggcagg ccccgccggc
     2221 gcagctcttc cagatagccc agggtcatcc aggtgagcat ccgcagcagc tccatcgggt
     2281 ccacgccggg gcggaatttg ccgaaatcaa tatttttgaa atatcccacc gcgccgtcca
     2341 gcgtctgctg cagccttgcg tccagctcag gggccagcct ctcgtcccgg gaggagtaga
     2401 cgcgcacggt gaactccatc agataggggt tttccgccac cagcttcagc tttttttccg
     2461 cgccgtgggc catcagctca aaaaaatcgc tgatctgggc aaacttttcg tccgccacct
     2521 gctccgccac catccgctcc acatgccggt acagataaaa atagagctct tttttgctgc
     2581 cgaaatagtg gaacaacagc ccttttgaga tgcccgcccg ggccgcgata tcgtcggtgg
     2641 aggcccgctt gtactcgttt ttgccgaacg cctccatggc tgcgttcacg atgcgccgcc
     2701 gcttttcttc cggcagggca aaaaactttt cattcaccgc cgcctccccc tttctgattg
     2761 acccgttcgg tcgaccactg tttccatcat agtgcattga ctcagattta caaggccccg
     2821 ggcaaaaatt ttttcacccc tttcccggct ctgtccccca tccatacgga aagagaacgc
     2881 cggttttggc ctgagccgaa ctcagccttg cattgagaac ccttttatga catactggtg
     2941 gtgtaaacga cagctttccg cgctgtgtgc tcagcgcgga aagcccgctt tttcaggagg
     3001 tgcttttttg aataagacat tgtttgaaaa cggcctggtc atcacagtgg atgaccagga
     3061 caccgtctac cgcaagggct atctgctggt ggagggcgac aggatcgccg ccgtgggtga
     3121 gggggattat gccggtgacc gcgccggggt cacggtggtg gacgcggacg gcaaggccgt
     3181 gctgcccggc atcgtggacg cccacaccca tgtggcgggc agcctgttca aaggcctgct
     3241 ggaggacgac cccaacggct tctacggctt tgccctgccc atggagggcc agctgacgcc
     3301 ggaggacgtc taccatctct ctcgcctggg cgcctgggag tgcatgcagg cgggcgtcac
     3361 caccgtcaac gagatctatc actatgtgct ctcggtggca aaggcggtgg acgaggtggg
     3421 cctgcgggcc gtcatcagcc agaacatcgt ggatgtggac atcggcgcgc tgcgctataa
     3481 cgactacacc cgctaccccg ccaagggcga agcctttgtc gaggacaata tccgcctgat
     3541 tgaggagtac cacggcaagg acaacggccg catcacctgc cgttttggcc ctcacgccac
     3601 cgacacggtc tcgatggagc tggcgaaaaa atgcgtggag ctgggcaaaa aatac
//
LOCUS       DBMDYA010000228         3652 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5844_length_3652_cov_1.648831, whole genome shotgun sequence.
ACCESSION   DBMDYA010000228 DBMDYA010000000
VERSION     DBMDYA010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5844_length_3652_cov_1.648831"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="ACZPFK_06630"
     CDS             <1..224
                     /locus_tag="ACZPFK_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR1406021.1"
                     /translation="GQRIHDYAQQVAKMKARNMDPADFESFLTAHKYGLPPHGGLGIG
                     LERFTSKLCDLANIRQASLFPRDLNHLAP"
     gene            499..747
                     /locus_tag="ACZPFK_06635"
     CDS             499..747
                     /locus_tag="ACZPFK_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406022.1"
                     /translation="MICLISYEPLRQLLKERGLSSYYLRNKCGNMNLDSKTIKRLMND
                     ESVSINTINALCNILKCDIKDIMIFQPDDCFISKDDRE"
     gene            complement(1175..1825)
                     /locus_tag="ACZPFK_06640"
     CDS             complement(1175..1825)
                     /locus_tag="ACZPFK_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406023.1"
                     /translation="MLQDIPYSKLQKNERSYKMMLLRDQHRATFAQIAEKYGVSLVRA
                     QQIYRQTKIMQIHLYLNHLAVTLKQSDCLQMQKVYDKAYKSYQDWGCVCAYLEKRYRR
                     ILESYRAGEPGMPAAFLKALPPLRPHLTAKEVTHIVKLREEEKMSYRAIGEELRITPA
                     KAKYTYDWFYHQLVLALLRERQKDAPDKQEERALWNHYFSSYKTPKQRYDALVNDS"
     gene            complement(2134..3387)
                     /locus_tag="ACZPFK_06645"
     CDS             complement(2134..3387)
                     /locus_tag="ACZPFK_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406024.1"
                     /translation="MNPFELKPAKVEDGLMDWKTLYPQPYDKNEADPYTKVRIILMTG
                     TEFEANWFSHQFHRHCADNDLRRELALSRRVEQQQQKQLACLKPIDETQLETTIAYEQ
                     LAVDLTAILAQRDPDPYVVKTMDLALLEDFDHLYRYADLLEMEQGVHAERLVGRYTEI
                     MPGRPTISEHRHPYDSVRRYCDFKTAAPITKLDVSIITAAEQQTMNYYMNLGAFYGSD
                     RGRKLYQEIGMIEEQHVTQYGALIDPNCTWLESLLMHEYIECYLYYSCFEDETDRRIK
                     KLWEMNFEKEVAQLHAAAKLLQKYEKKSWQQVLPDGAFPELLNFSEQKEYVRRVLAQT
                     ATQTARREDFVEVDSLPDDAPFFRYQDTVNPNAREVPSHRVIRDAISELGQDYRFEES
                     PSPIPALRSRKKDNTTLGRTKGEGK"
     gene            complement(3405..3569)
                     /locus_tag="ACZPFK_06650"
     CDS             complement(3405..3569)
                     /locus_tag="ACZPFK_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406025.1"
                     /translation="MEGKNEKRPPDPGHGSGAVDSILNMPPSHTDPNGSYTGRPQDPD
                     EVPVQDADDL"
BASE COUNT          761 a          955 c          990 g          946 t
ORIGIN      
        1 gcggccagcg catccacgac tatgcgcagc aggtggcaaa gatgaaagcg cgcaatatgg
       61 acccggcgga ttttgagagc tttctcaccg cgcacaaata cggtctgccg ccccacggcg
      121 gcttgggcat cgggctggag cggttcacct caaagctctg cgatttggcc aacatccgcc
      181 aggccagcct cttcccccgt gacctcaacc acctggcgcc gtaaagctgc cggaatcgcc
      241 gccccccgta tggggggctt ttttgttgtg cagagtgatg gttacccttg tagggatgga
      301 agcgccgtga tgtgagcctc gcttcggaga tttttgcctg gaggaaaagc tttaggaggt
      361 atgcaaagat cacgctgtcc tttttgctct ctgtcggcac ccccgcagat tcaaggctct
      421 tttatgatct ttctatatga ggagtattcc tcataaccgt gcatgctaat tacatgggat
      481 aattaaaaaa gcaaaggggt gatttgtctg atttcatacg aacctttaag acagctgtta
      541 aaagaaagag gacttagctc ctactatcta agaaataaat gcggaaatat gaatttggat
      601 agtaaaacaa ttaaaagact gatgaatgat gagtccgtat ctataaatac aataaacgcc
      661 ttatgtaaca ttcttaaatg cgatattaag gatattatga tatttcagcc tgacgactgt
      721 ttcatatcaa aagacgatag agaataagca agggatattc cgataccaga gaggaatatc
      781 ccttgcttat tttttagagc atctggtcag tccccctgcc gggaccattg agataggccg
      841 ttttttggac agccgtttcg attgccgccc tacggcggct ggggcattgg gccagaatgg
      901 ttcacttcaa agctctgcga cctggccaac atccgtcagg ccagtctctt cccccgcgac
      961 ctcaaccact tggagccgta aagctgccga aatcgccgcc cccccgtatg gggggctttt
     1021 ttgttgtgcg gagtgatgtt tacccttgta gagatggaag cgccgtgatg tgagcctcat
     1081 ttcggagacc ggcacctggg gaaaaagctt taggacgcat gcaaaggtca cgctgaaaag
     1141 aaacctgaaa aaagcctgtt tgcaggtgga cgttctagct gtcgtttacc agagcgtcat
     1201 aacgttgctt gggggtctta tatgaggaaa agtaatggtt ccacagagct ctttcttctt
     1261 gtttatccgg cgcatccttt tgccgctccc tgagtaaggc caataccagc tgatgataaa
     1321 accaatcata agtatatttt gcttttgcgg gggtaatgcg tagttcttct ccgatagcac
     1381 ggtaagacat tttttcttct tctcgcagtt tgacgatatg ggtcacctcc tttgcggtca
     1441 aatgcggccg cagcggaggc aaggccttta gaaaggcggc gggcatcccg ggctccccgg
     1501 cccgatagct ctccaaaata cggcggtatc tcttttccag gtaggcgcag acgcagcccc
     1561 agtcctgata acttttatag gccttatcgt aaactttttg catctgcaag caatcggatt
     1621 gtttcagcgt caccgcaagg tggttgaggt aaaggtgtat ttgcatgatc tttgtctggc
     1681 gatagatctg ctgggcccgc acaagggata ccccatactt ttcggcgatt tgtgcaaagg
     1741 tggcacggtg ctggtcgcgc agcagcatca ttttatagga gcgctcgttt ttttgcaatt
     1801 tgctgtaggg gatatcctgt aacatatgga cttcaccttt cctcacaaaa gtttttctcc
     1861 gaactgcaca gcaagatttt aaaataaatt ctcactttga aatgtggata agaactgggt
     1921 gccgcattga tttaaacctt atattataat ggaacagtgg aaaagatgac atttagaaga
     1981 tgtcaaataa aaatattttt cgatacatcg accggtttat ggactattgt caataaaaaa
     2041 gcagccgcta agcggctgct tttacgacct cgattttaga aatacactct gtgctgtgtg
     2101 gctccatttt ctagggctgc gccacaacgc attctatttc ccctcgccct ttgtgcggcc
     2161 cagggtggtg ttgtcctttt tgcggctgcg cagcgcaggg atggggctgg ggctctcctc
     2221 aaagcgatag tcctgcccca gctcgctgat ggcgtcgcgg atgacccggt ggctgggcac
     2281 ctcgcgggcg ttggggttca ccgtgtcctg atagcggaag aacggcgcgt cgtccggcag
     2341 gctgtccacc tcgacaaaat cctcccgccg cgcggtctgg gtcgccgtct gcgccagcac
     2401 ccggcgcaca tactcctttt gctcgctgaa gttcagcagc tcggggaaag cgccgtccgg
     2461 gagcacctgc tgccagctct ttttctcata tttctgcagc agctttgccg ccgcgtgcag
     2521 ctgggccacc tctttttcaa agttcatctc ccacagcttt ttgatgcgcc ggtcggtctc
     2581 gtcctcaaag caggaataat agaggtagca ctcgatatat tcatgcatca gcaggctttc
     2641 cagccaggtg cagttggggt cgatcagcgc cccgtactgg gtgacgtgct gctcctcgat
     2701 catgccgatc tcctggtaca gcttgcggcc ccggtcgctg ccgtaaaacg cgcccaggtt
     2761 catataatag ttcattgtct gctgctccgc ggcggtgatg atgctcacgt ccagcttggt
     2821 gatgggcgcg gccgtcttga aatcgcagta ccggcgcacg ctgtcatagg ggtggcggtg
     2881 ctccgagatg gtcgggcggc cgggcatgat ctcggtatag cggcccacca gccgctctgc
     2941 gtgtacgccc tgctccatct ccagcaaatc ggcatagcgg tacagatggt caaaatcctc
     3001 cagcagggcc aggtccatgg tcttgaccac ataggggtcc ggatcccgct gggccaggat
     3061 ggcggtcagg tctacggcca gctgttcgta ggcgatggtg gtctccagct gggtctcgtc
     3121 gatgggcttc aggcaggcca gctgcttttg ctgctgctgc tccacccggc ggctcagcgc
     3181 cagctcgcgc cgcaggtcgt tgtcggcgca gtgtcggtgg aactgatggc tgaaccagtt
     3241 cgcctcaaat tcggtgccgg tcatcaggat gatgcgcacc ttggtatagg ggtcggcctc
     3301 gtttttgtca tagggttggg gataaagggt tttccagtcc atcaggccgt cttccacttt
     3361 tgccggtttc agttcaaaag ggttcatctg tttgttcctc cttgtcaaag gtcgtccgcg
     3421 tcctgcaccg gcacctcgtc cgggtcctgc gggcggccgg tgtaactgcc gttggggtcg
     3481 gtgtggctcg gcggcatgtt gaggatacta tccaccgcgc cgctcccgtg tccggggtcc
     3541 gggggccgct tttcattttt gccctccatc ctttcacctc cccgcactgc tctgatgtat
     3601 atatttttct ctgagcggtg agaatatata ctgaagaggt gaacccattg ac
//
LOCUS       DBMDYA010000229         3646 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5857_length_3646_cov_1.321862, whole genome shotgun sequence.
ACCESSION   DBMDYA010000229 DBMDYA010000000
VERSION     DBMDYA010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_3646_cov_1.321862"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="ACZPFK_06655"
     CDS             complement(<1..330)
                     /locus_tag="ACZPFK_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1406026.1"
                     /translation="MQDNGKVIALLIDAENVSPKYIKAIVDEVAVYGTPAYKRIYGDW
                     TSNDMANWKRVLLEHNLTPIQQFSYTQGKNASDSAMIIDAMDILYANNVDGFCLVSSD
                     SDFTKLAS"
     gene            596..2017
                     /locus_tag="ACZPFK_06660"
     CDS             596..2017
                     /locus_tag="ACZPFK_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HIR1406027.1"
                     /translation="MIPYPAHVEIDLSKFAPGLPEEQLEARYGLPRQVRFCRKCVMSN
                     QKPNSCHEFEHTPHSVKKSMHIAADGVCDACVACADKVGKIDWQDRERQLRELCRCYR
                     RSDGGYDCLVPGSGGKDSFYAAHLLKYKYGMHPLTVTWAPHLYTPWGWRNFQAWIHAG
                     FDNYLCTPNGRTHRLLTRLATENLFHPFQPFILGQKQLAPKMAAKFGIPLVFYGENEA
                     EFGNPLADNESALRDERFFATNDRDHIYLGGVSLQQLEEEFKVDPADLAIYLPSDTSD
                     IEKNHIQVHYLGYYVKWHPQGAYYYAVEHGGFQPSPERTVGTYSKYNSIDDKIDDFFY
                     YTTYIKYGVGRATYDAAQEIRNDEITREEGVALVKKFDGEFPARFADEIFRYLSLDEK
                     SFPEARRCFEHPQMDLGYFMELADRFRSPHLWMWQDGGWKLRHTPYEGESACGYGAPA
                     KALCRAKNTPARRGEADTDGEIG"
     gene            2001..2768
                     /gene="hisF"
                     /locus_tag="ACZPFK_06665"
     CDS             2001..2768
                     /gene="hisF"
                     /locus_tag="ACZPFK_06665"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1406028.1"
                     /translation="MAKSDKIRLVARLEVKGDHLVKGIQLEGLRKLGDPHAFARGYYE
                     AGIDEILYLDIVASLYERNNLSHIVRRTTGDVFIPITVGGGLRSVEDVRGILQQGADK
                     AAINTAAVKDPSLITRVAEAYGSQCMVLSIQAKRRRDGPGWEAYYDNGREHSGLDVVE
                     WAKRGAELGAGEILLTSVDREGLGRGMDHELIRAVTAAVSVPVIAAGGAAGPDDVALA
                     AGEGASAVALSSLLHYKRMEVGTLKAALAARGVGVRA"
     gene            2765..3409
                     /gene="hisH"
                     /locus_tag="ACZPFK_06670"
     CDS             2765..3409
                     /gene="hisH"
                     /locus_tag="ACZPFK_06670"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1406029.1"
                     /translation="MRHVVLIDYGLSNLLSVQRAFEHVGAEVEIARGPAAVLGAQALV
                     LPGVGAFRDGMAGLKKLGLIGPIRQKAAAGTPLLGICLGMQMLFDEGEEFGLRPGLGL
                     IPGRVVKLPARDAAGAPLRVPQVGWNGLLPPPGRADFAQSALRAVQPGGEVYFVHSYE
                     AKPACPADRLADTLYGGRRVCAAARRGSVTGCQFHPEKSGEVGLSVLRQFLAEG"
BASE COUNT          645 a         1104 c         1273 g          624 t
ORIGIN      
        1 cgaggccagc ttggtgaagt cgctgtccga cgacaccagg caaaagccgt ccacgttatt
       61 ggcataaagg atgtccatgg cgtcgatgat catggcgctg tcgctggcgt ttttgccctg
      121 ggtatagctg aactgctgga tgggggtgag gttgtgctcc agcagcaccc gcttccagtt
      181 ggccatgtcg ttgctggtcc aatccccgta aatgcgctta taggccgggg tgccgtacac
      241 cgccacctcg tccacgatgg ctttgatata tttcggggag acgttctccg cgtcgatgag
      301 cagcgcgatg accttgccgt tgtcctgcac aggcttcgcc ctcctttttc tctgttttct
      361 ttattgtacc acaaagcggg cggcgcagtg cggcggagcg aaaaaaatat tgaaatcggc
      421 gcggcgctgt gctataatgc cggatatcaa caacggcgtt gatcgcgggg gcggggcccc
      481 ggcggtcacg ccgttgtttt ttgttcggtc ccacccgcag ccgggcgggg cgcaaaagcc
      541 gctccccggc ccgcaccagg ggccgcgcgg catgtcagtt caggaggtaa tgttgatgat
      601 accctatccc gcccatgtgg agatcgatct ctcaaaattt gcgcccggcc tgccggagga
      661 gcagctcgag gcccggtacg gcctgccgcg gcaggtgcgg ttttgcagga aatgcgtcat
      721 gagcaatcag aagcccaact cctgccacga gtttgaacac accccccaca gcgtcaaaaa
      781 gagcatgcac atcgcggcgg acggcgtctg cgacgcctgc gtggcctgcg cggacaaggt
      841 ggggaagatc gactggcagg accgggagcg ccagctgcgg gagctctgcc gctgctaccg
      901 caggtctgac ggcggctacg actgcctggt gcccggctcg ggcggcaagg acagctttta
      961 cgccgcccat ctgctaaagt acaaatacgg catgcacccg ctcaccgtca cctgggcgcc
     1021 gcacctctac accccctggg gctggcggaa ctttcaggcc tggatccacg cggggttcga
     1081 caactatctc tgcacgccca acgggcgcac ccaccggctg ctcacccggc tcgccaccga
     1141 gaacctgttc catccgtttc agccctttat cctggggcag aagcagctgg cgcccaagat
     1201 ggcggcaaag ttcggcatcc cgctggtttt ttacggcgag aacgaggccg agttcggcaa
     1261 tccgctggcg gacaacgagt cggccctgcg ggacgagcgc tttttcgcca ccaacgaccg
     1321 ggaccacatc tacctgggcg gcgtctcgct gcagcagctg gaagaggagt tcaaggtcga
     1381 cccggcggac ctggcgatct atctgcccag cgacaccagc gacatcgaaa aaaaccatat
     1441 ccaggtgcac tatctgggct actatgtcaa atggcacccg cagggggcct attactatgc
     1501 ggtggagcac ggcggcttcc agcccagccc ggagcgcacg gtgggcacct attccaaata
     1561 caactccatc gacgacaaga tcgacgactt tttctattac accacctata tcaaatacgg
     1621 cgtcggccgc gccacctacg acgcggcgca ggagatccgc aacgatgaga tcacccggga
     1681 ggagggcgtg gcactggtga aaaagtttga cggcgagttt ccggcccggt ttgcggacga
     1741 gatcttccgc tatctgtcgc tggacgagaa aagctttccc gaggcgcggc gctgctttga
     1801 gcacccgcag atggacctcg gctatttcat ggagctggcg gaccgcttcc gctcgccgca
     1861 tctctggatg tggcaggacg gcgggtggaa gctgcgccat accccctatg agggggagtc
     1921 ggcatgcggt tacggcgccc cggcaaaggc gctgtgccgg gcaaaaaata ccccggcgcg
     1981 gagaggggag gcggacacgg atggcgaaat cggataagat acgcctggtg gcccggctgg
     2041 aggtcaaggg cgaccatctg gtgaaaggca tccagctgga ggggctgcgc aagctgggcg
     2101 accctcacgc ctttgcccgg ggctattatg aggcgggcat cgacgagatc ctgtatctgg
     2161 acatcgtggc cagcctttac gagcgcaaca acctgtcgca tatcgtgcgg cgcaccaccg
     2221 gcgatgtttt catccccatc acggtgggcg gcggcctgcg cagcgtggag gatgtgcgcg
     2281 gcatcctgca gcagggggcc gacaaggcgg ccatcaacac cgccgccgtc aaggacccct
     2341 ctctgatcac acgggtggcc gaggcctacg gcagccagtg catggtgctg tcgatccagg
     2401 ccaagcgccg ccgggacggc cccggctggg aggcctacta tgacaacggg cgcgagcatt
     2461 cggggctgga tgtggtggaa tgggcaaagc ggggcgcaga gctgggcgcg ggcgagatcc
     2521 tgttgaccag cgtggacagg gagggcctgg gccggggcat ggaccatgag ctgatccggg
     2581 ccgtgacggc cgccgtgagc gtgccggtga tcgccgcggg cggggccgcg gggccggacg
     2641 atgtggccct ggccgccggg gagggcgcgt ctgcggtggc gctttccagc ctgctgcact
     2701 ataaaaggat ggaggtcggc acgctgaaag ccgccctggc cgcccgcggc gtgggggtga
     2761 gggcatgagg catgtcgttc tgatcgatta cggcctgtcc aacctgctca gcgtacagcg
     2821 ggcctttgag catgtggggg ccgaggtgga gatcgcccgc gggcccgccg cggtgctggg
     2881 ggcccaggcg ttggtgctgc ccggggtggg ggcgttccgc gacggcatgg ccgggctgaa
     2941 gaagctgggc ctcatcgggc cgatccggca gaaagccgcc gccggcacgc cgctgctggg
     3001 catctgcctg ggcatgcaga tgctgtttga cgagggcgag gagttcggcc tccggccggg
     3061 cctggggctg atcccgggac gggtggtgaa gctgcccgcc agggacgccg cgggcgcgcc
     3121 cctgcgggtg ccccaggtgg gctggaacgg gctgctgccg ccgccgggac gggcggattt
     3181 tgcccaaagc gcgctgcggg cggtgcagcc gggcggcgag gtctatttcg tccacagcta
     3241 tgaggcaaag cccgcctgcc cggcggatcg gctggcagat accctgtatg gggggcggcg
     3301 cgtctgcgcc gcggcccgcc gcggcagcgt caccggctgc cagtttcacc cggaaaaaag
     3361 cggcgaggtg gggctgtccg tcctgcggca gtttctcgca gagggatagg acgccaaaac
     3421 ggcaaagcgg ggggcagact ccctttccag ataaaaacag ggctttgcgt ttggccggct
     3481 ttgttgaggg caggcatccg gggccgtctc tcttgacgga aaattgcggg gtggaaaaag
     3541 cgcgtttccc acaaggggag gcagtgcccg gtgccggggc ggcggataag gcggagctga
     3601 aagaggtttc tcagcagggg cgcgccccct ggcgggaaga ggcttt
//
LOCUS       DBMDYA010000230         3642 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5863_length_3642_cov_1.542502, whole genome shotgun sequence.
ACCESSION   DBMDYA010000230 DBMDYA010000000
VERSION     DBMDYA010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5863_length_3642_cov_1.542502"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /gene="atpE"
                     /locus_tag="ACZPFK_06675"
     CDS             complement(<1..181)
                     /gene="atpE"
                     /locus_tag="ACZPFK_06675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1406030.1"
                     /translation="MLEKAIILAGCAVGAGLALIAGIGPGIGEGYAVGKACEAIGRQP
                     ECKGDVTSTMLLGCAI"
     gene            complement(275..1024)
                     /locus_tag="ACZPFK_06680"
     CDS             complement(275..1024)
                     /locus_tag="ACZPFK_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1406031.1"
                     /translation="MELDITGARIYFTIPVLGGIPITETIVNAWIVMAVILGACIFLT
                     RNLKVRATSRRQIIAEFLVQKAQSFVTENMGRRYLYYTPFIAALFSMSLLSSLLSLFG
                     LYPPTSDLSTTMAWALLVFVLITYTKIKTGGVGGYLKGFTQPIFVLTPFNILSELATP
                     ISMAFRHFGNVVAGSVITALVYGALAAASHALLGLLPGAVGETLSAIPILQVGVPAVL
                     SVYFDLFSSFMQAFIFCMLTTLYIANAADDS"
     gene            complement(1014..1436)
                     /locus_tag="ACZPFK_06685"
     CDS             complement(1014..1436)
                     /locus_tag="ACZPFK_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR1406032.1"
                     /translation="MKVQPAVKKETLHIAAGTGLLTAVMLLVFLLLGRLTLPVVLGAL
                     LGACFAVLNFFLLGLTIQGALKTGSESRGKLKMQLSYTLRMLLTLAVLLVGFSVPWFH
                     WLATVLPLLFPRLTIFAMQLLGLYQPEKAEGGDGPDGA"
     gene            1747..2127
                     /locus_tag="ACZPFK_06690"
     CDS             1747..2127
                     /locus_tag="ACZPFK_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HIR1406033.1"
                     /translation="MGFLGNVLWFLLGGWAVGLGWVIAGALWCLTIVGIPIGVQCFKF
                     AGLAFWPFGKEVAYGGGAVSLLANLLWLIFSGIPMALADAALGLVFCATIVGIPFGLQ
                     CFKLAKLALMPFGASVLTQVRILT"
     gene            2566..3198
                     /locus_tag="ACZPFK_06695"
     CDS             2566..3198
                     /locus_tag="ACZPFK_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1406034.1"
                     /translation="MLYGIGESPVCCGAAPQAVAAPRKGLAVLLPGFGYGPDKPLLHY
                     ARKAALAAGFEVLAVEYGPLPREKSDLARSVRDAFGPALAAAAAQLDRVLAGPPFRSV
                     FFVSKSFGTLVAGELTARRPELAAKNFYLTPLPASLPYLAQPGGQRLAAAGTADPFVT
                     AADRARMRAVLGDRLLVFEGADHSLEVPGDPLAGVAILEQVVAALCGMMR"
     gene            3368..>3642
                     /locus_tag="ACZPFK_06700"
     CDS             3368..>3642
                     /locus_tag="ACZPFK_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1406035.1"
                     /translation="MYKIFIVEDDPAIASALAAHLGRWGYEAVAARDFTRITEEFAAA
                     APQLVLMDITLPYYDGYHWCRELRRISKVPIVFLSSASDNLNQIMAL"
BASE COUNT          632 a         1160 c         1200 g          650 t
ORIGIN      
        1 caatggcgca gccaagcagc atggtggatg tcacgtcgcc cttgcactcg ggctggcggc
       61 cgatggcctc gcaggccttg ccgacggcgt agccttcgcc gatgccgggg ccgatgcccg
      121 cgatgagggc gaggccggcg cccacagcgc agcctgccag gatgattgct ttttccaaca
      181 tagaggtttc ctcctacata aaaatagttt gggttatttt cagcgccctt gcacgcagcg
      241 ggcggcggaa aaacaaacgg gagcttcgcg tccgtcagga atcgtcggcc gcgttggcga
      301 tatacagcgt tgtcagcatg cagaaaatga acgcctgcat aaagctggag aacaaatcga
      361 aatacacgct gagcaccgcc ggcacgccca cctgcaggat ggggatggcc gagagcgtct
      421 cccccaccgc accgggcagc agcccgagca gcgcgtggga ggcggcggcc agcgccccgt
      481 agaccagcgc cgtgatgacg ctgcccgcca ccacgttgcc gaaatggcgg aacgccatcg
      541 agatcggggt ggccagctcg ctgaggatgt tgaaaggggt gagcacgaag atgggctggg
      601 taaagccctt gagatagccg cccacgcccc ccgttttgat cttggtgtag gtgatgagca
      661 caaagaccag cagcgcccag gccatggtgg tggacaggtc gctggtgggg gggtagagcc
      721 cgaacaggct gagcaggctg gagagcagcg acattgagaa cagagccgcg atgaacgggg
      781 tgtaatagag atagcgcctg cccatgttct cggtgacaaa gctctgcgcc ttctgcacca
      841 gaaactcggc gatgatctgc cgccgggagg tggcccgcac tttcaggttg cgggtgagaa
      901 aaatgcacgc ccccaggatg accgccatca caatccaggc gttgacgatg gtctcggtga
      961 tggggatgcc ccccaaaacg gggatggtga aatagattct ggcgccggtg atatcaagct
     1021 ccatccggcc catccccccc ttccgccttt tcgggctgat agaggcccag cagctgcatg
     1081 gcaaagatgg tcagccgcgg gaagagcagc ggcagcacgg tggcgagcca gtggaaccag
     1141 gggaccgaaa agcccaccag cagcaccgcc agcgtcagca gcatgcgcag cgtgtaggac
     1201 agctgcatct tcagcttgcc gcggctctcg ctgccggtct tcagcgcgcc ctggatggtg
     1261 aggcccagca aaaagaagtt gagcaccgca aagcaggcgc ccagcagcgc ccccagcacc
     1321 accggcagcg tcagccggcc cagcagcaga aagaccagca gcatcaccgc cgtcagcagc
     1381 ccggtgccgg ccgcgatatg cagcgtctcc tttttgaccg ccggctgcac tttcatccca
     1441 cacgccccct ctcctgctct cgtcccgttt tcagtatgcc ccgccgccgc gcccgcaagc
     1501 cgggcgattc gggcaaattg cgaaaaagag aaccatggat ttgatgatat cacacaaaaa
     1561 aggcatcgtc aaacaaaaaa ctttgttcgt tatttgacaa acaaagtttt ttgtcgtcca
     1621 cccgtcccct gattcgtatt atatccgtat gagatgctca aatcgtcatt ttttgatgca
     1681 atttatcatt gttctttcca tcttttatcg cgacggaacc gccgcgatcc cacggaggtg
     1741 ctgaatatgg gatttttggg aaatgtgttg tggtttttgc tggggggctg ggccgtgggc
     1801 ctgggctggg tgatcgccgg ggcgctctgg tgcctgacca tcgtgggcat ccccatcggg
     1861 gtacagtgct tcaagttcgc cgggcttgca ttctggcctt tcggcaagga ggtggcctac
     1921 ggcggcggcg cggtgtcgct gttggccaat ctgctctggc tgattttcag cggcatcccc
     1981 atggcattgg ccgacgcggc gctggggctg gtgttctgcg ccaccatcgt gggcatcccc
     2041 tttggcctgc agtgcttcaa gctggcgaaa ttggccctga tgcctttcgg cgccagcgtg
     2101 ctgacgcagg tgcgcatcct gacctagccg caggggagac agacggcccg ttgccggcgt
     2161 cgcgtgccgg tttgcggcgc gggccgtttg gggcgcgtgc cggcttgtcg cgcttgtcga
     2221 cgtaggggcg cataccggcc cgggaggggc gggccgacgc gggtgcgccc cctgttctcg
     2281 ggctgcagct ccggcaaaag cacggcagcg gccgtctttt ctgcgccgcc cgacgccgga
     2341 gggcgaaaga gcccgggggc cggcgaaaga gccgacaggc cgagcgccgc actggcgccc
     2401 cgttttccac cgcggcggcc ctcctaaaaa aagcttccct gtggtataat agccgcaaga
     2461 ggttttgggc cgcagcccgt ttggcagcgc ggggccgtcg ccgtccctgc gcgcacggcg
     2521 gccgcagccc aaaagcggga acaggcagag gaggcgctga gcaggatgct ttatgggatc
     2581 ggcgaaagcc ccgtttgctg cggcgctgcg ccacaggccg tagccgcccc gcgcaagggg
     2641 ctggcggtgc tgctgcccgg cttcggctac gggccggaca agccgctgct gcactatgcc
     2701 cgcaaggccg cccttgccgc gggcttcgag gtgctggcgg tggaatacgg ccccctgccg
     2761 cgggagaaat cggacctggc ccgctcggtg cgggacgctt tcggcccggc gctggccgcg
     2821 gccgcggcgc agctggaccg ggtcctggcc ggccccccgt tccgcagcgt cttttttgtc
     2881 agcaaaagct tcggcaccct ggtggcgggc gagctgacgg cgcgccgccc cgagctggcg
     2941 gcaaaaaatt tttacctcac cccgctgccc gcctccctgc cctatcttgc gcagcccggc
     3001 gggcagcgcc tcgccgccgc gggcacggcc gacccgttcg tgacggcggc cgaccgcgcg
     3061 cggatgcggg ccgtgctggg ggatcgcctg ctggtctttg agggggcgga ccactcgctg
     3121 gaggtgcccg gcgacccgct ggcgggcgtg gccattctgg aacaggtggt cgcggcgctc
     3181 tgcgggatga tgcggtagcc ccgtccgcgc aaaagcccgt cggcggccac cgccgccgtt
     3241 cgcaaggggc aaagccgcgg cggcctccca tcttttccaa aacgcccgca gggcggcacg
     3301 gcaaaggggc ggtacgctct ttttctgttt gcgctcttcc atttcatctg taaaaaagga
     3361 gcctgccttg tataagattt ttattgtgga ggacgacccg gccatcgcct cggcgctggc
     3421 cgcgcacctg ggccgctggg gctatgaggc ggtggccgcc cgggacttta cccgcatcac
     3481 cgaggagttt gccgccgccg ccccgcagct ggtgctgatg gacatcaccc tgccctatta
     3541 cgacggctat cactggtgcc gcgagctgcg ccgcatctcc aaggtgccga tcgtcttttt
     3601 gtcctcggcc agcgacaacc tgaaccagat catggccctc ag
//
LOCUS       DBMDYA010000231         3637 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5868_length_3637_cov_1.350962, whole genome shotgun sequence.
ACCESSION   DBMDYA010000231 DBMDYA010000000
VERSION     DBMDYA010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5868_length_3637_cov_1.350962"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="ACZPFK_06705"
     CDS             complement(<1..1154)
                     /locus_tag="ACZPFK_06705"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1406036.1"
                     /translation="MDVKHPRNLTMLTDFYEITMAAGYFEEGYEDKTCVFDMFFRRVP
                     DGGGFAICAGLEQLIESLDNLSFTEEDLSYLRACGCFGEPFLDYLRNFRFCCSVWAIP
                     EGTPIFPGEPIVTVEGPAIQAQLVETMLLVNMNHQSLIATKANRIVRAAAGRPVAEFG
                     ARRAQSYDAAVLGARAAYIGGVASTSCVMAARDFGIPASGTMAHSWVQMFPSEYEAFK
                     KYAELYPQGCTLLVDTYNVIKSGVPNAIRVFDEVLKPRGIRPAGVRIDSGDIAYLSKR
                     TRRLLDEAGYQDVGIVASNSLDEYIVRDLILQDAKVSSFGIGENLITSKSDPVFGGVY
                     KLAAIKEGDVYLPKIKVSESAEKLTTPHRKAVWRIYDNETGNAMADYITI"
     gene            complement(1239..>2155)
                     /locus_tag="ACZPFK_06710"
     CDS             complement(1239..>2155)
                     /locus_tag="ACZPFK_06710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1406037.1"
                     /translation="FFYLMNNRAKELGCTNTNFTCAHGLYGMDQNHYSSARDMYLIAK
                     ACYDKGALFMDVATSLSYTIPASNKHPTSYNIRNTNAMMRQGTEVYRSYIQGIKTGST
                     PEAGFNLITTAKKDGQTYMLVVMGTPYEKDEYGYGMAFSVTAQIYDWVFDSLSVRPAL
                     DTTQDITEIKVKYCSEQDTLKLRPASDLMTLLPIESDETTINKEFHLPEAVTAPIEAG
                     DVVGSVTLSLAGEEIGTVDLIASQSLERNTALYVIAKIGEFFSSLYFKVLLVLILITV
                     AVYVGYILWVTHKNKKMGKVNRRRSRKL"
     assembly_gap    2156..2165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2166..2557)
                     /locus_tag="ACZPFK_06715"
     CDS             complement(<2166..2557)
                     /locus_tag="ACZPFK_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1406038.1"
                     /translation="MKKIFCSLLALLTLSLTLLAAPAGAVTFTPPFEPNADAVYLVNL
                     DTGIVVYEKNADKKRPPASLTKLMTTLLLLENVPDLDGTRITAPGYIFDELFGVASST
                     ADIRPYEEVSARDLLYAMMLPSANEAAST"
     gene            2972..>3637
                     /locus_tag="ACZPFK_06720"
     CDS             2972..>3637
                     /locus_tag="ACZPFK_06720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1406039.1"
                     /translation="MIYVTADLHGDPDRLREAAAKRLKRQDTLIVLGDFGFLWRGDKA
                     EQKLLKWLGKRPYTLLFLDGAHENYDLLREYAVESYAGGRARRISGRLYHLLRGEIYT
                     IEGKKLLCFGGGESEDRLDREEGETWWRSELPTADELDNCMARLAENGGRVDYILTHT
                     PPYRLRRFLLGESAETNRLETFLDQVADQADYGCWYFGRYHLDRAVSPKAVAVYRKIV
                     PLWT"
BASE COUNT          659 a         1034 c         1240 g          694 t
ORIGIN      
        1 aagatggtga tatagtcggc catggcgttg cccgtctcgt tgtcgtagat gcgccagacc
       61 gccttgcggt gcggcgtggt cagcttttcg gcggattcgc tcaccttgat cttgggcaga
      121 tagacgtccc cctctttgat cgcggccagc ttgtacacgc cgccgaagac cgggtcgctc
      181 ttgctggtga tcaggttttc gccgatgcca aagctgctca ccttcgcgtc ctgcaaaatc
      241 aggtcccgca cgatgtattc gtccagggag ttgctggcca cgatgcccac gtcctggtag
      301 cccgcctcgt ccagcaaacg gcgggtgcgc ttggacagat aggcgatgtc gcccgagtcg
      361 atgcgcacgc ccgcgggccg gatgccccgg ggcttcagca cctcgtcaaa gacccggatg
      421 gcgttgggca cgccgctctt gatgacgttg taggtgtcca ccagcagcgt gcagccctga
      481 gggtacagct cggcgtattt tttgaaagcc tcgtactcgg aggggaacat ctgcacccag
      541 gagtgagcca tggtgcccga ggcggggatg ccgaaatccc gcgccgccat cacgcagctg
      601 gtgctggcca cgccgccgat ataggccgcc cgcgcgccca gcacggccgc gtcgtagctc
      661 tgggcccgcc gtgcgccgaa ctcggctacc ggccggccgg ccgccgcccg cacgatgcgg
      721 ttggccttgg tggcgatcag gctttgatgg ttcatgttga ccagcagcat ggtctccacc
      781 agctgggcct ggatggccgg gccctccacc gtgacgatgg gctcgccggg gaagatgggc
      841 gtcccctcgg ggatggccca gacgctgcag caaaagcgga agttgcgcag atagtcgagg
      901 aacggctcgc caaagcagcc gcaggcgcgc agatagctga ggtcctcctc ggtgaagctg
      961 aggttgtcca gcgattcgat gagctgctcc agcccggcac agatggcgaa gccgcccccg
     1021 tccggcaccc ggcggaagaa catgtcgaag acgcaggtct tgtcctcata gccctcttca
     1081 aaatagccgg cggccatggt gatctcataa aaatcggtga gcatcgtgag gttgcgcggg
     1141 tgtttgacat ccatgttcaa aactcctttt ctcccggcag ggggccgggt ccggcgcgct
     1201 tgcgcgcgtg cgggacgggc gggcgacggc cgtggggctc agagctttct tgaccggcgg
     1261 cggttcacct tgcccatctt tttgtttttg tgggtgaccc agaggatgta gcccacatag
     1321 acggccacgg tgatcaggat cagcaccaag agcaccttaa agtagaggga ggagaaaaat
     1381 tcgccgatct tggcgatcac atacagggcg gtgttgcgct ccaggctctg gctggcgatc
     1441 aggtccacgg tgccgatctc ctcccccgcc aaagagaggg tgacgctgcc caccacgtcc
     1501 cccgcctcaa tgggggcggt caccgcctcg ggtagatgaa actccttgtt gatggtggtc
     1561 tcgtcgctct cgatgggcag cagcgtcatc aggtcgctgg ccgggcgcag cttgagggtg
     1621 tcctgctcag agcaatattt caccttgatc tcggtgatgt cctgggtggt gtccagcgca
     1681 gggcgcaccg acaggctgtc aaagacccag tcgtagatct gggccgtcac tgaaaaagcc
     1741 atgccgtagc cgtattcgtc cttttcatag ggggtgccca tcaccaccag catataggtc
     1801 tggccgtcct ttttggcggt ggtgatcagg ttgaagcccg cctcgggggt ggagccggtc
     1861 ttgatgccct ggatatagct gcggtagacc tcggtcccct ggcgcatcat ggcgttggtg
     1921 ttgcggatgt tataggaggt ggggtgcttg ttgctggccg gtatggtata ggacaggctg
     1981 gtggcaacgt ccatgaacag cgcgcccttg tcgtagcagg ccttggcgat caaatacatg
     2041 tcccgcgccg aggaatagtg gttctgatcc atgccgtaca ggccgtgggc gcaggtgaaa
     2101 ttggtgttgg tgcagcccag ctctttggcc cggttattca tcagataaaa aaagtnnnnn
     2161 nnnnngtgct ggccgcctcg ttggcgctgg gcagcatcat ggcgtagagc aggtcccgcg
     2221 ccgagacctc ctcgtagggg cggatgtcgg cggtggagct ggccacccca aacagctcgt
     2281 cgaagatata gcccggcgcg gtgatgcggg tgccgtccag gtccgggacg ttctccagca
     2341 gcagcagggt ggtcatcagc ttggtgagac tggccggggg gcgctttttg tcggcgtttt
     2401 tctcgtatac cacgatgccg gtgtccaggt tcaccagata cacggcgtcg gcgttgggct
     2461 caaagggggg ggtaaaggtg accgcgcccg caggggccgc cagcagggtg agagagaggg
     2521 tcagcagggc caaaaggctg cagaaaattt ttttcatgaa cgctacctcg caggatatgg
     2581 aatgatggaa aggggcgtgc ggtttgtgtt ataatgggcg caagcgccca ttgaaaccgg
     2641 caagaccgca aagacccgcg gtcttgtggt ggccgccttg cggcgggatg gttataatgg
     2701 gcgcaagcgc ccattgaaac cggcaggacc gcaaaagacc ccgcggtttt gtggtggccg
     2761 cggcgcgggc ccggctgaaa gggcggcggg ctgcgtttcg gggcgcaggg gagaatcgcg
     2821 gccggccgcg cggaccgccg tccccctgag ggaaatcata atataatagt atagcattat
     2881 tttgtggaaa taaaaagtat ctgccggcgg cgcagtggga aagaaacggc tttcccggcc
     2941 ctgtgcgggg cggaaaggga ggggaacggg agtgatctac gtgacggcgg acctgcacgg
     3001 ggacccggac cggctgcggg aagcggccgc aaagcggctc aagcggcagg acaccctgat
     3061 cgtgctgggg gatttcggct ttttgtggcg cggggacaag gcggagcaaa agctgctcaa
     3121 atggctgggc aaaaggcctt ataccctgct tttcctggac ggcgcgcatg aaaattacga
     3181 tctgctgcgg gagtatgcgg tggagagcta tgcgggcggg cgcgcccgcc gcatcagcgg
     3241 caggctgtat cacctgctgc ggggcgagat ctacaccatt gagggcaaaa agctgctctg
     3301 ctttggcggc ggcgagagcg aggatcggct ggaccgggag gagggcgaga cctggtggcg
     3361 cagcgagctg cccaccgccg acgagctgga caactgcatg gcaaggctgg cggaaaacgg
     3421 cggccgggtg gattatatcc tcacccacac cccgccctac cgcctgcggc gctttttgct
     3481 gggggaatct gccgagacca accgtctgga aacctttctc gaccaggtgg cggaccaggc
     3541 ggactacgga tgctggtatt tcggccgcta tcacctggac cgggccgtca gccccaaggc
     3601 ggtggcggtc taccgcaaga tcgtcccgct ctggaca
//
LOCUS       DBMDYA010000232         3634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5872_length_3634_cov_1.633456, whole genome shotgun sequence.
ACCESSION   DBMDYA010000232 DBMDYA010000000
VERSION     DBMDYA010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_3634_cov_1.633456"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /gene="tsf"
                     /locus_tag="ACZPFK_06725"
     CDS             <1..664
                     /gene="tsf"
                     /locus_tag="ACZPFK_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1406040.1"
                     /translation="AKNDKFKSLVYGIARAVIDENPADVDALLKCKMEGGTVESALQD
                     RILVIGENMKIRRFTRYEGDCVAYIHGGGTHAVLVAFDTDLAGKPEFEACGKDVAMQI
                     AAVNPSYVGEADVPAEVIENEKKILLAQIENDPKLAGKPEQVKAKMVDGKIGKFYKEN
                     CLLDQPFVKDDKITVAAYLADCAKEQGGKISVAKFVRYEKGEGLEKKQEDFAAEIASM
                     VK"
     gene            1416..2186
                     /locus_tag="ACZPFK_06730"
     CDS             1416..2186
                     /locus_tag="ACZPFK_06730"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HIR1406041.1"
                     /translation="MEDREVYSLYFTGTGTSRTYADAVAGELGEAKSVDLTPKDLPME
                     LGMGAVAVFAAPVFGGRIPAAAVRRIRALAGNGAAAVALAVYGNRAYEDALLELKDLL
                     EEQGFRVVAAAALVAQHSMAPTLAAGRPDEADLKAAREFGREVAAKLAAMPQREKNAA
                     LQVPGDRPYRLYNGVPFHPSAGKNCVRCGLCAQECPVGAIPAAAPERTDEKRCISCMR
                     CVTVCPQRARALGTLQEKAVTFKLAKACAERKAPELFL"
     gene            complement(2222..2623)
                     /locus_tag="ACZPFK_06735"
     CDS             complement(2222..2623)
                     /locus_tag="ACZPFK_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406042.1"
                     /translation="MHKSAPVRQKNGCGEAKKERKPANMQEDGERLVLPDKIKAARHR
                     RLAKDTATGKRVIAGAQDRQKAADAADLPFPPESSESQLLPKSHPGREPFTRTIKKTK
                     NKKCGEPPTKLFSVFFVFPASNPLKIRCINA"
     gene            2729..3082
                     /locus_tag="ACZPFK_06740"
     CDS             2729..3082
                     /locus_tag="ACZPFK_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1406043.1"
                     /translation="MDSAQELVNQLDQELRRGTLVLSVLSRLSSPQYGYSLVQELGER
                     GVGVDASTLYPLLRRLEKQGLCEASWETGGAKPRKYYLRTALGDEVYGVLSQHWRSVV
                     ESMDGLLGEGSEDER"
     gene            3072..>3634
                     /locus_tag="ACZPFK_06745"
     CDS             3072..>3634
                     /locus_tag="ACZPFK_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406044.1"
                     /translation="MRDDQSYIEEMTRRYIWAVVKHLPAKGRSDIETELKGLIEDMLA
                     ERTGGGRPTPKDLDVVLAELGAPYELAQKYIAQDRYLIGPELFPKYWLVLRIVLAAEV
                     FGLTLAAALTAALGGAAGIGAFVRWLANLCVGAAVAFGFVTLFFAVAERQGGRLRQQL
                     MHQDWKLSELPPVPPGKSRIPRGEPIAG"
BASE COUNT          717 a          988 c         1214 g          715 t
ORIGIN      
        1 cgccaagaac gacaaattca aatcccttgt ttacggcatc gcgcgcgccg tcattgacga
       61 gaatcccgcc gatgtggacg cgctgctgaa gtgcaagatg gagggcggca ccgttgagag
      121 cgccctgcag gaccgcattc tggtcatcgg cgagaacatg aagatccgcc gcttcacccg
      181 ctatgagggc gattgcgtcg cctacatcca cggcggcggc acccatgcgg tgctggtggc
      241 gttcgacacc gacctggccg gcaagcccga gtttgaggcc tgcggcaagg acgtggcgat
      301 gcagatcgcc gcggtcaacc ccagctatgt cggcgaggcc gacgtccccg ccgaggtgat
      361 cgagaacgag aagaaaatcc tgctcgccca gatcgagaac gacccgaagc tggccggcaa
      421 gcccgagcag gtcaaggcga agatggtcga cggcaagatc ggcaagttct acaaagagaa
      481 ctgcctgctg gaccagcctt tcgtgaaaga cgataagatc accgtggccg cttatctggc
      541 cgactgcgcc aaggagcagg gcggcaagat cagcgtcgcc aagtttgtcc gctacgagaa
      601 aggcgagggc ctggagaaaa agcaggaaga tttcgcggct gagatcgcca gcatggtgaa
      661 ataagcgcgt ttctgttctg gtccgttcca aaatttccat aaatccgtct gaaattaccg
      721 tgggcgggta ggggaccgag agacgcgagg gagctacatt gacgcatgaa agtgcgttga
      781 tgtagctctt tttttgtttg gatttggggg agagaagaag cttcctttgg ggcaacggag
      841 gcttcttttg gggtaaacga aagtgtcctt tggggcgctg agaagagccg gaacagagca
      901 gtcctacgcc atttctgcac ttcccgctac tttcttttgc cagcctcaac ccgccgccag
      961 cgtccgctcc tcccgtgttc tgcccaccaa agtccagggc ctctccgggc cgttcaggac
     1021 gcgcccctaa aaggcctcat atgcacccca aaagaggctt tttccccttg acctccctca
     1081 gaaatacggt aattatgggc ggatttgcgg aaaaatggaa cgcactttta cagtttccgc
     1141 cttataaaac gatgtttttg agagcaccgg gtcttttggc ccggtgctct ttgttgcctg
     1201 tgcgctgggc ctcttgttgc atttgcgccg cggcgtgtta gaataggggc aggcgcggca
     1261 ccgcggcggc gagggcggcg ggcaaaaggc ggcccctgcc gtggcgcggc agcgctttcg
     1321 tcccctgggc cgctgcagcg gacggggaga ggaagaattt aggggcagcg cctcgtcaca
     1381 ggggccacag ccctgttttg aaaggggaaa gtcagatgga ggatcgggaa gtttacagct
     1441 tgtacttcac cggcaccggc accagccgca cctatgcgga cgctgtggcc ggggaattgg
     1501 gggaggcgaa gagcgttgac ctgacgccga aagacctgcc catggagctg gggatggggg
     1561 cggtcgccgt tttcgccgcg ccggtctttg gcggccgcat cccggccgcg gccgtgcgcc
     1621 ggatacgcgc gctggcgggc aacggcgcgg ctgcggtggc gctggcggtc tacggcaacc
     1681 gcgcctacga ggacgcgctg ctggagctga aagacctgtt ggaagagcag ggctttcggg
     1741 tggtggcggc cgcggccttg gttgcccagc actccatggc gccgacgctg gccgccggca
     1801 ggccggacga ggccgatcta aaagcggccc gtgaatttgg acgcgaagtg gcggccaagc
     1861 ttgcggcaat gccgcagcgg gagaaaaacg ccgcgctcca ggtgccggga gaccggcctt
     1921 atcgcctgta caacggcgtc ccgttccatc cctcggctgg gaagaattgt gtccgatgcg
     1981 gcctttgcgc acaggagtgc ccggtggggg cgatccccgc cgccgcgccg gagaggaccg
     2041 acgagaagcg ctgcatttcc tgtatgcgct gcgtgacggt ctgtccgcag cgggcgaggg
     2101 cgctggggac gcttcaggaa aaagcggtca cctttaaact ggcaaaggcc tgcgcggagc
     2161 gaaaagcgcc ggaactgttt ctgtgatggg acggataacg gacaggagag cgcagcggcc
     2221 gctaagcgtt tatacagcgg atttttaatg gattcgaagc gggaaaaaca aaaaatacgg
     2281 agaacagctt tgtgggcggt tctccgcatt ttttgttttt tgtcttttta atggttcgcg
     2341 tgaatggctc gcgccctggg tgcgattttg gcaaaagctg gctctcgctt gattccggcg
     2401 gaaaaggcag gtccgccgcg tcagcagctt tctgtcggtc ctgcgcgcct gcgatgaccc
     2461 gcttccccgt cgccgtatct tttgccaacc gccgatgccg agcagctttt atcttatccg
     2521 gaagaaccaa gcgttctccg tcctcttgca tgtttgccgg ttttctctct tttttggctt
     2581 cgccgcagcc atttttttgc cgcactggcg cacttttgtg caaacactta ataatcacat
     2641 attaaaaata aaatttaaat ataaataata atatattgac aatagtgtgt gatatacagt
     2701 ataatgtgaa tacggaggtg agatgcggat ggattcggca caagagctgg tgaaccagct
     2761 tgatcaagag ctgcgccgcg gcacattggt gctcagtgta ctcagccgcc tctccagccc
     2821 acaatacggc tatagcctgg tgcaggagct gggggagcgg ggggtcggtg tggacgccag
     2881 caccctgtac ccgctgctgc ggcggctgga aaaacagggc ctgtgtgagg cgagctggga
     2941 gacgggcggg gcgaaaccgc gcaagtatta tctgcgcacg gccctgggcg acgaggtata
     3001 cggtgttttg agccagcact ggaggtcagt ggtggaaagt atggacggat tgttggggga
     3061 gggatcggaa gatgagagat gatcagagtt atatcgagga gatgacgagg cgatacatct
     3121 gggccgtggt caagcatctg cccgccaagg ggcggtcgga catcgagacg gagctgaaag
     3181 gcctgatcga ggatatgctg gccgaacgga caggaggcgg gcggcccacc cccaaggatc
     3241 tggacgtggt gctggcggag ctgggcgcgc cctatgagct ggcgcagaaa tacatcgcgc
     3301 aggaccgcta tctgatcggg ccggagctgt tccccaaata ctggctggtg ctgcggatcg
     3361 tgctggcggc cgaggttttt gggctgacgc tggccgccgc cctcaccgcc gcgctgggcg
     3421 gcgcggcggg gatcggcgct tttgtgagat ggctggcaaa cctctgtgtc ggggccgcgg
     3481 tggcctttgg cttcgtcacc ctgtttttcg ccgtggccga gcggcagggg ggcaggctgc
     3541 gccaacagct gatgcatcag gattggaagc tgagcgagct gccgccggtg cccccgggca
     3601 aatcccgcat cccgcggggg gagcctattg cggg
//
LOCUS       DBMDYA010000233         3633 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5875_length_3633_cov_2.046716, whole genome shotgun sequence.
ACCESSION   DBMDYA010000233 DBMDYA010000000
VERSION     DBMDYA010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5875_length_3633_cov_2.046716"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="ACZPFK_06750"
     CDS             <1..102
                     /locus_tag="ACZPFK_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1406045.1"
                     /translation="RKKIEAAPSSPRYIQTHVGVGYRMLRVEAAAEE"
     gene            complement(244..1284)
                     /locus_tag="ACZPFK_06755"
     CDS             complement(244..1284)
                     /locus_tag="ACZPFK_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1406046.1"
                     /translation="MRWKKNMEPIDHSCLHDILIEDEEGNIQPMDEPYFKAKEIAPGT
                     WQVLSDGDYTYVLEGDDELICIDGGSGAGNIRAFCQTLSDKPLYRLFNTHNHFDHTAN
                     NYLFDVVYMSEKCYAGRCQPFGEHAALDFPDDYPVVFLKDGDVVNLAGRELEVYNIEE
                     HCAGSLQFLDRKNRILFCGDELNGNFFDSRISVEHSFRNVQRWMSFRDAYDVLCAGNG
                     IHDASYVDKYYETLKYILEGHENEGVEFYKPYEDRRASISSKDGKPVYARRSPHMDSV
                     ADAMRAAGFEKHLELTEGRGCFCFMRKLTPDGLFDRQLERNGCRVCYYLNKIWDKQAE
                     ENSCTATLSAMK"
     gene            complement(1334..1771)
                     /locus_tag="ACZPFK_06760"
     CDS             complement(1334..1771)
                     /locus_tag="ACZPFK_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR1406047.1"
                     /translation="MLKGIPPVLSPDLLKTLAEMGHGDRIVLGDAYFASATMAKKNRL
                     LRADGVPSLALVDAILQLMPLDTWVDSCVFALGEAQGDGFAPAEMTRNYQEIIRRYEP
                     QAADNITLMDRFAFYDIAREAFAVVATGETEPYGCIILQKGVR"
     gene            complement(1802..3214)
                     /locus_tag="ACZPFK_06765"
     CDS             complement(1802..3214)
                     /locus_tag="ACZPFK_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR1406048.1"
                     /translation="MEKQTFGVIVTTRSFFPSHLVKTAREQITRKLEELGYGWVMVSE
                     QDTQHGAVLTFDEAKTCAALFKAHRDEICGIICVLPNFGEELGVAEAIDLAGLDVPVL
                     VQACDDDFDKLDMANRRDAFCGKISLCNNLYQRGIPFTDTTLHTCAIDSPEFTADLKK
                     FAAVCRVVGGLRGARIAMLGARPVAFNTVRYSEKILQAHKIAVQTVDMSEIIFAAQNY
                     PDGEKVRAKEAEIRAYGKIPAHIEDFKVTKQAKLCLAIEEYVDALQCQASTVQCWDSV
                     ENNYGCATCLAMSMMGEKGKPSACESDVLGAVSMLAARLAAGTAPALMDWNNNIREDR
                     DSCISLHCSNFPKSFFQNPEMEIECLDVLGSTLGQDKCFGACKAQVAPGPMTFVRLTT
                     DDTRGVMKMYVGEGEFGAEPIPTKGGVANCRVPHLQQLMKYICKNGYEHHVCFVRGHV
                     ADVLAEALSTYLGVEVYRHA"
     gene            complement(3273..>3633)
                     /locus_tag="ACZPFK_06770"
     CDS             complement(3273..>3633)
                     /locus_tag="ACZPFK_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406049.1"
                     /translation="PEAARLSGVKVNLYRALAFMLTAVFAGIGGILIVSRAGTVAAAA
                     GSSFLMPAIAAVNIGSAVGGRGKPNAIGTFVGAALIGVVENGLYALSFPYYAINVVKG
                     LILLLALVMSNYANKEN"
BASE COUNT          614 a         1152 c         1155 g          712 t
ORIGIN      
        1 cgcaaaaaga tcgaagccgc cccctcctcg ccccgctaca tccagaccca cgtgggggtc
       61 ggctaccgca tgctgcgggt agaggccgca gccgaggagt gagcggcgcg accgaggcaa
      121 gcgccgggga cgaacggtga agcggccccg cggcattatc tgtgcggcgg ggccgggttc
      181 caacaaaaga aaaagcgccg ggggcaaagc ccccggcgct ctcttttttt atctattttc
      241 aggtcatttc atggcggaca gggtggcggt gcagctgttc tcctccgcct gcttatccca
      301 gatcttgttc aggtagtagc agacgcggca gccgttccgc tccagctggc ggtcgaacag
      361 gccgtccggg gtcagcttgc gcatgaagca gaagcagccc ctgccctcgg tgagctccag
      421 gtgcttctca aaaccggcgg cacgcatcgc gtcggccacg ctgtccatgt gggggctgcg
      481 gcgggcgtag acgggcttgc cgtccttgct gctgatcgag gcgcggcggt cctcataggg
      541 cttgtagaac tccacgccct cgttctcgtg tccctccagg atgtacttca gcgtctcata
      601 gtatttgtcc acatagctgg cgtcgtggat gccgttgccc gcgcacagca cgtcgtaggc
      661 gtcccgaaac gacatccagc gctgcacgtt gcggaaagag tgctccaccg agatgcggct
      721 gtcgaagaag ttgccgttca gctcatcgcc gcagaacagg atgcggtttt tgcggtccag
      781 gaattgcagc gagcccgcgc agtgctcctc gatgttgtac acctccagct cgcggccggc
      841 caggttgacc acgtcgccgt ccttcaaaaa gaccaccggg taatcgtcgg gaaaatccag
      901 cgcggcgtgc tcgccaaagg gctggcagcg gccggcatag cacttttcag acatgtacac
      961 cacgtcgaac agatagttgt tggcagtgtg gtcgaagtgg ttgtgagtgt tgaacaggcg
     1021 gtagagcggc ttgtcggaca gggtctggca aaaagcgcgg atgttgcccg cgcccgagcc
     1081 gccgtcgatg cagatcagct cgtcgtcgcc ctccagcaca taggtgtagt cgccgtcgct
     1141 gagcacctgc caggtgcccg gggcgatctc ctttgccttg aaatagggtt cgtccatcgg
     1201 ctggatattg ccctcctcgt cctcgatgag gatgtcgtgc aggcaggagt ggtcgatggg
     1261 ctccatgttc tttttccagc gcataagtat aagcctccca gcttttattg gttgtcgttc
     1321 gaaaaggcgg gccttagcgc acgccctttt gcaggatgat gcagccgtag ggctcggtct
     1381 cgccggtggc caccaccgcg aaagcctccc gcgcgatgtc gtagaacgca aagcggtcca
     1441 tcagggtgat gttgtccgcg gcctgcggct catatctgcg gatgatctcc tgatagttgc
     1501 gggtcatctc agcgggcgca aagccatcgc cctgggcctc gcccagcgcg aacacgcagc
     1561 tgtccaccca ggtgtccagc ggcatcagct gcaaaatggc gtccaccagc gccagcgagg
     1621 gcacgccgtc cgcccgcagc agccggttct tttttgccat ggtggccgag gcgaaatagg
     1681 cgtcgcccag cacgatcctg tctccgtgtc ccatctcggc cagcgttttc agcaggtcgg
     1741 gggagagcac cggaggtatc cctttcagca tatcgttccc tttccgcctt tgcggctgtg
     1801 ttcaggcatg acggtagacc tccacgccca gataggtgga gagcgcctcg gccagcacgt
     1861 cggccacatg gccgcgcaca aagcagacgt ggtgctcata gccgtttttg cagatgtact
     1921 tcatcaactg ctgcagatgg ggcacgcggc agttggccac cccccccttg gtggggatgg
     1981 gctcggcgcc gaactcgccc tcgcccacgt acatcttcat cacgccgcgg gtgtcgtcgg
     2041 tggtcagccg cacaaaggtc atcgggccgg gggccacctg ggccttgcag gcgccgaagc
     2101 acttgtcctg ccccagcgtg ctgcccagca cgtccaggca ctcgatctcc atctccgggt
     2161 tctggaaaaa gctcttgggg aagttggagc agtgcagcga gatgcagctg tcccggtcct
     2221 cgcggatgtt gttgttccag tccatcaggg ccggggccgt gccggcggcc agccgggcgg
     2281 ccagcatgct cacggcgccc agcacgtcgc tctcgcaggc gctgggcttg cccttttcgc
     2341 ccatcatgct catggcaagg caggtggcgc agccgtagtt gttctccacg ctgtcccagc
     2401 actgcacggt gctcgcctgg cactgcagcg cgtccacata ttcctcaatg gcgaggcaga
     2461 gcttggcctg cttggtgacc ttgaagtcct cgatgtgggc cggaatcttg ccgtaggcgc
     2521 ggatctccgc ctccttggcc cgcacctttt cgccgtccgg gtagttctgg gccgcgaaga
     2581 tgatctcgga catgtccacg gtctgcaccg caatcttgtg ggcctgcagg attttttccg
     2641 agtagcgcac cgtgttgaac gccaccggcc gggcgcccag catggcgatg cgggccccgc
     2701 gcagcccgcc cacaacccgg cagacggccg caaacttttt gaggtccgcg gtgaactcgg
     2761 ggctgtcgat ggcgcaggtg tgcagggtgg tgtcggtgaa agggatgccc cgctggtaga
     2821 ggttgttgca caggctgatc ttgccgcaga aagcgtcgcg gcggttggcc atgtccagct
     2881 tgtcaaagtc gtcgtcgcag gcctgcacca gcaccggcac gtccagcccg gccaggtcga
     2941 tggcctcggc cacccccagc tcctcgccga agttgggcag cacgcagatg atgccgcaga
     3001 tctcgtcccg gtgcgccttg aacagcgcgg cgcaggtctt ggcctcgtca aaggtgagca
     3061 cggcgccgtg ctgggtgtcc tgttcgctta ccatcaccca gccgtagccc agctcctcca
     3121 gcttgcgggt gatctgctcc cgcgccgttt tcaccagatg agagggaaaa aagctgcggg
     3181 tggtgacgat cacgccaaag gtctgttttt ccattgtagt tccgctcctt tatcagtgtt
     3241 gccgccgtcc ggcggcgcct ctctttgagg ctttagttct ctttgttggc gtagttgctc
     3301 atcaccagcg ccagcagcag gataaggccc ttgaccacgt tgatggcgta gtaggggaac
     3361 gagagcgcat acaggccgtt ttccaccacg ccgatcaggg ccgcgcccac aaaggtgccg
     3421 atggcgttcg gcttgccgcg gccgcccacg gccgagccga tgttcaccgc cgcgatggcg
     3481 ggcatcagaa aagaggagcc cgccgcagcg gccacggtgc cggcgcggga gacgatcagg
     3541 atgccgccga tgcccgcgaa gaccgcagtg agcataaaag ccagggcccg gtacagattt
     3601 accttgacgc cggacagccg cgcggcctcg ggg
//
LOCUS       DBMDYA010000234         3627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5886_length_3627_cov_1.288429, whole genome shotgun sequence.
ACCESSION   DBMDYA010000234 DBMDYA010000000
VERSION     DBMDYA010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5886_length_3627_cov_1.288429"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(307..383)
                     /locus_tag="ACZPFK_06775"
     tRNA            complement(307..383)
                     /locus_tag="ACZPFK_06775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(347..349),aa:Val,seq:cac)
     gene            704..2188
                     /locus_tag="ACZPFK_06780"
     CDS             704..2188
                     /locus_tag="ACZPFK_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1406050.1"
                     /translation="MGKIGFDNELYMKTQKEHILERIKGAQGKLYLEFGGKLFDDYHA
                     ARVLPGFDVNAKIKLLYELRDMAEIIFVVSAWDIEKTKIRADLGITYDMDVLRLIDDI
                     THLGIEVNSVVITRYSGQPAADAFLAKLTMRGVKNYVHKPIKGYPTDIDYICSENGFG
                     ANPYIETGKPLVVVTGPGPGSGKLATCLSQVYHETVRGVPAGYAKFETFPIWNLPLKH
                     PVNLAYEAATADLADLNMIDPFHLEAYGETTVNYNRDVEAFPIVRNTLSRISGGAFDY
                     KSPTDMGVNMIGAAIVDDQAVREASCQEIIRRYFAAKCDHRQGKGSEEAVEKIRMVMQ
                     QVGVTPNDRAVVNPALEKSRRTGMPAAAIQLGDGRVVTGKASELMSASSSVVLNAIKA
                     LTNIDDDLTLISPIILEPIVKLKTEILGGRSTLLKLDDVLTALSISAATNTLTARAME
                     ALPQLRDCEMHSTQMLHSGDEGTLRRLGLRLTCEAIYPGKDLFF"
     gene            2561..>3627
                     /locus_tag="ACZPFK_06785"
     CDS             2561..>3627
                     /locus_tag="ACZPFK_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR1406051.1"
                     /translation="MKIGLDIGSTTIKCVVLDKNDAIVYQSYERHFSHIVGKAAEVLR
                     RIESEVTGRHAVLAISGSAGMGLADACKLPFVQEVFATRVAAQKLSPSTDVIIELGGE
                     DAKILFLTGGMDVRMNGSCAGGTGAFIDQMATLLKLTPEQMNEAALRSEKLYTIASRC
                     GVFAKSDVQPLLNQGARKEDVAASIFAAVVNQTIGGLAQGRPIEGNVMYLGGPITFLS
                     KLKASFDDALGVAGVLPENSLYYVALGAAFYSDEAFDLEDVIASLENYSATADYATSD
                     PLFRSQAEYDAFAARHARATVPRGELAGARGPVHIGIDAGSTTVKAAVIDGQGRLLYS
                     SYQPNSGNPVPIIRSVLLDLY"
BASE COUNT          712 a         1152 c         1138 g          625 t
ORIGIN      
        1 taaaaaaaga ccggcccgcc cggcggccgc cgggcgggtg gattgtctgc cgatttgggc
       61 ccgcaacccc tgcccccctc ggcaggacag gggcggcggg ccacggggcg acgggccttg
      121 aggcggggca acccggccgc ctgcgggacc ccgggccctc gcggcccggt gggggagaag
      181 cggtagggct gcgtcccgcc gttggccggg gcgcggcgtc cgggccgcga tgcgcgcggc
      241 cgaaaaacgg caaacaaaga atccgggcct ctctcccgca ggaaaaaggc ccggatccta
      301 ttgatttggt ggagacggag ggactcgaac ccgcgacctc tcggatgtga accgaacgct
      361 ctaaccagct gagctacgcc tccataagat gctttaatat aataccacag gtttcaaaaa
      421 aagggaagcc ctttttgaaa aaaatttgag tcttttttgc ggcggcggtt ttgtcctttt
      481 tcacttgaaa aaagcaggac aaaatgatac tcttatagag ttatagcgac tgatattgcg
      541 ccgggccgca cgggcccggg ccgacggccc gccgtctgtg cgggggcggc ggacagagga
      601 cagggccttg gcacgcatgc cgcgcgcggg ccctgttttg tgggtctggg gacgggcagg
      661 ctgcggcggg acgggaaaaa tacaggacgg atggggtgcg cgtatgggca aaatcgggtt
      721 tgacaatgag ctgtatatga agacacagaa agagcatatc ctggagcgga tcaagggcgc
      781 ccagggcaag ctctacctcg agttcggcgg caagctgttt gacgactacc acgccgcgcg
      841 ggtgctgccc ggctttgatg tgaacgccaa gatcaagctg ctgtacgagc tgcgggacat
      901 ggcggagatc atttttgtgg tgtcggcctg ggacatcgaa aagaccaaga tccgcgccga
      961 cctgggcatc acctacgata tggacgtgct gcggctcatc gacgacatca cccacctggg
     1021 catcgaggtg aacagcgtgg tcatcacccg ctattccggc cagccggcgg cggacgcctt
     1081 tctcgccaag ctgaccatgc gcggggtcaa aaattacgtc cacaagccca tcaagggcta
     1141 tcccaccgac atcgactaca tctgcagcga aaacggcttt ggcgccaacc cctatatcga
     1201 gaccggcaag ccgctggtgg tcgtcaccgg ccccggcccg ggcagcggca agctggccac
     1261 ctgcctctcg caggtctacc acgagacggt gcgcggcgtg cccgccggct atgccaaatt
     1321 tgagaccttt cccatctgga acctgcccct caagcacccg gtgaacctgg cctacgaggc
     1381 ggccaccgcc gacctggccg acctgaacat gatcgacccg ttccatcttg aggcctacgg
     1441 cgagaccacc gtcaactaca accgggacgt ggaggccttt cccatcgtgc gcaacaccct
     1501 gtcccgcatc agcggcgggg cgttcgatta caagagcccc accgacatgg gggtcaacat
     1561 gatcggcgcc gccatcgtgg acgaccaggc ggtgcgggag gccagctgcc aggagatcat
     1621 ccggcgctat ttcgccgcca agtgcgacca ccgccagggc aagggcagcg aggaggcggt
     1681 ggagaagatc cgcatggtca tgcagcaggt gggggtcacc cccaacgacc gcgccgtggt
     1741 caaccccgcg ctggaaaaat cccgccgcac cggcatgccc gcggcggcca tccaattggg
     1801 ggacggccgc gtcgtcaccg gcaaggccag cgagctgatg agcgccagct cgtcggtggt
     1861 gctcaacgcc atcaaggcgc tgaccaacat cgacgacgac ctcaccctga tcagccccat
     1921 catcctggag cccatcgtca agctcaaaac cgagatcctg ggcggccgca gcaccctgct
     1981 caagctggac gacgtgctga ccgcgctctc gatctcggcg gccaccaaca ccctgaccgc
     2041 ccgcgccatg gaggcgctgc cccagctgcg ggactgcgag atgcactcca cccagatgct
     2101 gcactccggc gacgagggca ccctgcgccg gctggggctg cggctgacct gcgaggccat
     2161 ctatcccggc aaggacctgt ttttctgagc ccgccgcacc gccctgcggc cgccgcaaaa
     2221 aagtgatgaa aaaaccagct gcgcagcggc tggtttttta gctttctgcg ccgccccctc
     2281 tgcgccgcag ggggcggttt cttttgccgc cccgcgggga gaagacgttt cacagcacag
     2341 gaactgcact tttcatacct tatataagat gaaatgccgg ccaaacggat atttttggga
     2401 caaaatgcaa aaattgaggc gccggcagcc ggatttttta cactctacag gttgtttttc
     2461 ccggcccgga ggtgtataat agtacacatt aatgaatctt atttccgaca aaatttcact
     2521 ttattttttg tcgaattttc acaattgcga gggtttccat ttgaaaatag gtctcgatat
     2581 cggctccacc accatcaagt gtgtggtgct ggacaaaaac gacgcgatcg tctatcagtc
     2641 ttatgaacgg catttttccc acatcgtggg caaggcggcc gaggtgctgc gccgcatcga
     2701 gagcgaggtc accggccgcc acgcggtgct ggccatctcc ggctcggcgg gcatggggct
     2761 ggccgacgcc tgcaagctgc cctttgtgca ggaggttttc gccacccggg tggccgcgca
     2821 aaagctgtcg cccagcaccg acgtcatcat cgagctgggc ggtgaggacg ccaagatcct
     2881 gtttctcacc gggggcatgg acgtgcgcat gaacggcagc tgcgcgggcg gcaccggcgc
     2941 ctttatcgac cagatggcga cgctgctcaa gctgacgccc gagcagatga acgaggcggc
     3001 gctgcgcagc gaaaagctgt acaccatcgc ctcccgctgc ggcgtgttcg ccaaaagcga
     3061 cgtgcagccg ctgctgaacc agggcgcccg caaggaggac gtggccgcct ccatcttcgc
     3121 ggccgtggtc aaccagacca tcggcggcct ggcccagggg cggcccatcg agggcaacgt
     3181 gatgtacctg ggcgggccga tcaccttttt gtccaagctg aaagccagct ttgacgacgc
     3241 gctgggggtg gccggcgtct tgccggaaaa ttcgctctat tacgtggcgc tgggcgcggc
     3301 cttttactcc gacgaggcct ttgacctgga ggacgtgatc gccagcctgg aaaactattc
     3361 ggccaccgcc gactacgcca ccagtgaccc gctgttccgc tcccaggcgg agtacgacgc
     3421 ctttgcggcc cggcacgcgc gggcgacggt gccccgcggc gagctggccg gggcgcgggg
     3481 cccggtacat atcggcatcg acgcgggctc caccaccgtc aaggcggcgg tcatcgacgg
     3541 gcaggggcgg ctgctctatt ccagctacca gcccaacagc ggcaacccgg tgcccatcat
     3601 ccgctcggtg ctgctggatt tgtatga
//
LOCUS       DBMDYA010000235         3619 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5899_length_3619_cov_1.748437, whole genome shotgun sequence.
ACCESSION   DBMDYA010000235 DBMDYA010000000
VERSION     DBMDYA010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_3619_cov_1.748437"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            26..101
                     /locus_tag="ACZPFK_06790"
     tRNA            26..101
                     /locus_tag="ACZPFK_06790"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Lys,seq:ctt)
     gene            1371..1943
                     /locus_tag="ACZPFK_06795"
     CDS             1371..1943
                     /locus_tag="ACZPFK_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406052.1"
                     /translation="MGERMHKPGYMLFLESRGIRPGGYFRAQGAFGESCFRVTRDCRL
                     LDLDGAPAALDENSLWEQLESLAPISRREFLSTREALWEVRRAADGRLTDAQLTPLVR
                     QFTQEYTEAHAEGRWKGFATWDEETLRRILMRAAALLPGIKSSREARKKAAELFREVV
                     AAGLESGIVRRPASARDKREETEGKNPPKP"
     gene            2327..2737
                     /locus_tag="ACZPFK_06800"
     CDS             2327..2737
                     /locus_tag="ACZPFK_06800"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR1406053.1"
                     /translation="MNDEEKIKYFYEVVTTHGLVNEIEEYVSADCTTRVGEKTFYAGV
                     EGMREHMVGVRHTYPDLKMVIIRQYRDGDYIISEFIAEGTHKGEWLGMKPTGKKLTFT
                     GVDIDRVVDGKIVEHGGAMNTFETLFEEGIIKAV"
     gene            3116..>3619
                     /locus_tag="ACZPFK_06805"
     CDS             3116..>3619
                     /locus_tag="ACZPFK_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1406054.1"
                     /translation="MQYRQLGRTGLQVSEIGMGCEGFIGKSAAEVAALVDEMETAGVN
                     CIDLYAPDPSMREALGKALRGRREKFVLQGHLCTIWKDGQYKRTREIGEVKAGFADLL
                     ARLGTDHVEIGMIHYVDAMEDWEAVKNGPVMAYARQLKAEGKIGCIGLSSHNPAPARA
                     AAESGLVD"
BASE COUNT          896 a          812 c         1105 g          806 t
ORIGIN      
        1 gctgcgccgc ccgcgcagct tccatgcacc tctagctcag ttggtagagc agctgactct
       61 taatcagcgg gcccagggtt cgagtccctg gaggtgcacc aaaaatcgac aagcctttac
      121 ggggcttgtc gatttttata tttggagata tccatgaatt ttctctagcc cgccagtaaa
      181 gcctcttaaa aagggacaca agtactttaa gcagggcagg agtactggaa ctggaaccgc
      241 ttggtgagat gcgattttag cgaactgttt cgggtagctg taaaaatctc tgcacggccg
      301 taatcggccc gcagcatccg cggcgctcct ctgccactgt ccccacaccg cggggagaca
      361 gagtgtttgc aagcacatgg tggcggcttt ctttgcggct tttctggcag aggcggaaaa
      421 gtgttatgtc agggtgctgt tctaagaaga ggaaaacagg cgatatcaaa aaagttggcc
      481 gccaagttgg taaaatatgc atggcttaaa ggaaaacgct caaaacaagc ttttaaaagc
      541 attggccggt ggcccgcaat ggctgtggga gcaatgactc cgagataata tcgagcttga
      601 gtgaaaaacg gccgatcttg ttaccgcaga ggtgaagacc ggtatttttt gaaagacgaa
      661 agattggcag cgacgacaaa atcatccgca gcaagctcct ctgcggatga tttttataca
      721 ataaaatttt tatagagcct cttggcccct ttttatggcc ctcctgtttg caaagtgttt
      781 cggtgtcacg gcattctgca attaaggggc ggagaggaaa cgtgacgaaa agcgagcgcg
      841 ggcagtgcgg agagcgctgc ggcatgtttt atctgaatca ggtattatcg cgagacggca
      901 aatactttcc cgcgtatgat ccgggcttct ctcgcttttg acgatagcgc cgcggtatat
      961 tacaaaaagc tttgtctgcg tttaaggggc cgtgtgattg acagagtaaa gagacgatag
     1021 cgccctgcag ggaggacaaa gaatcaaaca acggcgtggg agctcctcgc accggccgcg
     1081 ccaatgtgag ctcagcgtcc aggcggtaaa aaacaccctt tcgcctgtac ggagagcggc
     1141 ggctgtggta gaataataac cagggtaaag agtattttgc ggtgctttgc ggttttttca
     1201 gctccatttt ttcgaagaag acggtttttt gcccgtgctc cacgttatcc cgcacagccc
     1261 tgctgcactt tctgcactta acagcgtctt ggatgccgca aaaaagagag ggcatagtgt
     1321 attcgtggac tcgtttttaa aaagcgatgc gaaaagagga gtgcggggaa atgggagagc
     1381 ggatgcataa gccagggtat atgctctttt tagagagccg cgggatccgt ccgggaggat
     1441 atttccgcgc gcagggcgcg tttggggaaa gctgctttcg ggtcacccgg gactgccgcc
     1501 tgctggacct ggacggcgcc ccggcggcgc tggacgagaa cagcctttgg gagcagctgg
     1561 agagccttgc gccgatttcg cgccgggagt ttctctccac gcgggaagct ttgtgggagg
     1621 tgcgccgcgc cgccgacggc cggctcaccg atgcgcagct gacgccgctg gtgcggcagt
     1681 tcacgcagga gtatacagag gcccatgctg agggccgctg gaaagggttc gcaacctggg
     1741 atgaggagac gctgcgccgc atcctgatgc gggccgcggc gcttttgcca gggataaaaa
     1801 gcagcaggga agcgcgcaaa aaggcggcgg agcttttccg ggaggtggtt gccgcagggc
     1861 tggaaagcgg tattgtaaga cggccggcct cggcaagaga taaaagagag gaaacggagg
     1921 ggaaaaatcc ccccaagccc tgacggggat gcctctcaaa agcgtctcca aagggacggc
     1981 gggcgaagtg ctgtttcgcg ggaaacgctg tcaagcagcg gcttgctgcc gcgccggcaa
     2041 tttttcggcc gggcaaacag gacgaaagaa gagccttgtt ttccatcggc gtcggatttc
     2101 cggctcacgg gaagtggatc gggatacggg gctttctcaa ttaggcgtga acgagaaagc
     2161 gttggaacag cacctgagag gtatccccgc aaaagaggcg tgttcgcata attttaaaag
     2221 attatttcgt gtttcatgaa gttgttgaaa gttggttatg agtctgtcgc cttggatcaa
     2281 taaagcacac tcactaaact gggatttgtt tgagaggaag agttttatga atgacgaaga
     2341 aaaaatcaaa tatttttatg aggtagttac cacccacggt cttgtaaatg aaatagaaga
     2401 atatgtgtcg gcagactgca caacaagggt tggagaaaaa actttttatg caggtgtcga
     2461 aggcatgcga gagcatatgg tgggtgtaag acatacttat ccggatttga aaatggtaat
     2521 tatccgacaa tatcgcgatg gagattatat catctctgaa tttattgcag aaggaactca
     2581 taaaggtgaa tggttaggca tgaagcctac tggaaagaaa ttaacgttta ctggtgtgga
     2641 tatcgacaga gtcgttgatg ggaagatcgt tgaacatggt ggtgctatga atacctttga
     2701 aacgcttttt gaagaaggga taatcaaagc cgtataacgg ttagctgtcc gtctgtgggg
     2761 tggattgaat tccccttgtc agaataagat gtacctcttc tttggccgtt tttaatagtg
     2821 tcaagaagcg ttttgcaatg cgcacaaaac gatcttccga ttttttgtct ccacgaaaac
     2881 ctgaagagcc tgcttgcagg gagcttccat ggattccctg acaagggtgt gccgctgaat
     2941 tgccgaacgc ctcgtggggg gaactaaaaa atagccgcag gctatttttc cggccatccg
     3001 gccagtcctc tggccggggc catcaagata agttttttat tgcggagcaa tgccatcaag
     3061 ataagttttt ttacggcaat caaaaaataa gaaagataaa atgggaggca gtgagatgca
     3121 ataccggcaa ttgggccgca cgggcctgca ggtcagcgag atcggcatgg gctgtgaggg
     3181 atttatcggc aagagcgcgg cggaggtcgc cgcactggtg gacgagatgg agacggcagg
     3241 ggtcaactgt atcgacctgt acgcgcccga tccctccatg cgggaggcgc tgggcaaggc
     3301 gctgcggggc cgcagggaaa aatttgtttt gcaggggcat ctgtgcacga tctggaaaga
     3361 cggccagtac aagcgtacac gggagatcgg ggaggtgaaa gccggctttg cggatctgct
     3421 ggcccggctc ggcaccgacc atgtggagat cggcatgatc cattatgtgg atgcaatgga
     3481 ggactgggaa gcggtaaaaa acgggccggt catggcctat gcccggcagc tgaaagcgga
     3541 gggaaagatc ggctgcatcg gcctctccag ccacaatccc gccccggcgc gggcggcggc
     3601 ggagagcggc cttgtggat
//
LOCUS       DBMDYA010000236         3618 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5902_length_3618_cov_1.390105, whole genome shotgun sequence.
ACCESSION   DBMDYA010000236 DBMDYA010000000
VERSION     DBMDYA010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_3618_cov_1.390105"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            504..782
                     /locus_tag="ACZPFK_06810"
     CDS             504..782
                     /locus_tag="ACZPFK_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406055.1"
                     /translation="MAEKTACNYFLLQEKVSILKKTGAQQKLCACFLACWSENVGSIV
                     PCRKDIVICRAPHGGKTVRSLPACSRSKGGLTAQDSTLSPASAAWRGR"
     gene            complement(793..3603)
                     /locus_tag="ACZPFK_06815"
     CDS             complement(793..3603)
                     /locus_tag="ACZPFK_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406056.1"
                     /translation="MTVTGGVFGSGASCDAGSTRLVTLRSYGRPIETGGTVTGATRTL
                     AAVQRADRVTLIDTHVQLSGQSDAANANQTAKYALNRIGDKGDIDGNGNGVVLQGGST
                     LILDSAVIETANFKSADADGNPVPKEGLQTTPNTVRFNAGVVFQVACTDDAGKTVYGA
                     VSGYAYMLAGDTAEAYAYARVNTGANSGDGGFQAPGESEELAYHTAGEEYRYWQVKGA
                     NATADRYTVLTAQTLKAGDNGYGSDYSVASGSIELPPVDKETAYSIESVVLPGGITLV
                     EAAKNGLDAAWVTAAEVNGALTTTAVELAEEQKKISDSPLDTFGLFMRIGTGFQTQDV
                     SGKVISDESVQSGGKNTVIGQKTPQTQNNGTPRIDFYLTYANNISVSKDLGTIKLVLK
                     REEDGARTTVNVAIVTKGSSLVDQTVELYATQSGSYTGRLIIPSGDSRNLKLSNISQT
                     ANLVSAAAEPTEEQFSLTLRPIKFPGWSSENLMTDAYDLHAGAPTAVPLGTTDSRYEA
                     VIELVLKNAPGFQAKEHPDEVTFQLSDGENNATVTLKIYWKDSIVSDVAVMSGRQYNG
                     FSEQSAAATVSQKSAVTAAFTMGGGETAESLWLELRRKDSAEKLTLPAGTQLTLLGPS
                     GFYSYQAKGEETTLPLSRFSKMWEESALSGNLLSGQIWTVIVDFGVADTLPNGDYTLR
                     LRNETGADSMGAEFTVNNSEMTVSLSDGKSFSHGKCALVLSVSAGSDTRVAGGATAVL
                     SLPEGQSFPVGTVFLCRDKTYFPQGGRVYLPLAGAADYNIEIDTTGTAGMREGSYQLT
                     ASVHPAGYGAGMAKVVTGTGAHTIQADPVYAISAAPADGEDRVVTVGTTTEVKFNVEA
                     SVQNADGEQQKIQVAVQKKGLGGRYENEDASLWSVSGNDSFQGAAAQQITVTIPASAA
                     AGTYRLLFTLGGKTASCNLIFQ"
BASE COUNT          688 a         1124 c          974 g          832 t
ORIGIN      
        1 ttggatgaca ttcgccatcg taaaggtctt gccgctgccg gtgaccccca gcatggtctg
       61 ctcccgatag cccttgagca ccccctcggt cagcttggcg atcgcctggg gctgatcccc
      121 cgtcggctga tagggcgcga ccagtttgaa agacttgtac tcctcttcca tcgctccacc
      181 tccctgccct ccccgcaggc agcgccgccg cccagagaga gatcaataaa attcgtcccg
      241 atcagacttt aaaagcgctc aaagacgtga taccattagg atggtacgaa atgaagcgct
      301 aaattcgtgt tttttaaagc aaacgcccga tccggcagac aagaactcaa tattatatta
      361 tagcacaaac catctaattt tgaaaggcct gtccctagca aaataattgc gccgcaccga
      421 cacagcccct ctgcggcgta ttttttattg ctgcgcaata aaaaacgatt tattttaatg
      481 gccacggccc ggggacgggc cggatggccg aaaaaacagc ctgcaattat tttttattgc
      541 aagaaaaagt ctctatttta aagaaaacag gcgcacagca aaagctgtgc gcctgttttc
      601 ttgcttgttg gtctgaaaat gtgggcagca ttgtgccctg ccgaaaagac atcgttattt
      661 gccgcgcacc tcatggaggt aaaacggtga ggtctttgcc cgcctgcagc cggtcaaaag
      721 gcgggcttac cgcacaggac agcaccctct cacctgcctc ggccgcgtgg agagggcgat
      781 aaagcgatgg gttcactgaa agatgagatt gcaggatgcg gtcttgccgc ccagggtaaa
      841 cagcagccga taggtgcccg ccgccgcact tgccggtatc gtcacggtaa tctgttgcgc
      901 agcagctccc tggaatgaat cgttgccgct cacgctccac agggaggcgt cctcgttttc
      961 gtatcgtcct ccaaggcctt ttttctgtac ggcaacctgg atcttctgct gttccccatc
     1021 ggcgttttgt accgacgcct ctacattaaa cttcacttca gttgtggtcc ccactgtgac
     1081 gacacggtcc tcgccgtccg ccggagccgc gctgatcgca tacaccgggt ctgcctggat
     1141 cgtgtgcgca ccggttcctg tgaccacttt tgccataccg gcgccgtagc cggcgggatg
     1201 aaccgaggct gtgagctgat agctgccctc cctcataccg gcggtgccgg tggtatctat
     1261 ttcaatattg tagtcggcgg cgcctgctaa tggcagataa acccggccgc cctgcgggaa
     1321 atacgttttg tcccggcaga gaaacaccgt ccccaccggg aagctttgtc cctcgggcaa
     1381 cgacaacacc gctgtcgcac cgcccgcaac acgggtatcc gagcccgcgg aaacgctcag
     1441 gacaagtgcg catttcccgt gggaaaagct tttgccgtcg gacagcgaaa ccgtcatctc
     1501 actgttgttt accgtaaact cggcgcccat cgaatcagcg cccgtctcgt ttctcaggcg
     1561 cagggtataa tcgccgttcg gcagcgtatc cgccacgcca aagtcgacga tgaccgtcca
     1621 tatctgaccc gacaaaagat tgccggaaag cgcgctctcc tcccacattt tcgaaaaccg
     1681 ggacaagggc agcgtcgtct cttccccttt cgcttgataa gagtaaaagc cgcttggtcc
     1741 cagcagcgtc agctgtgtcc ctgccggcag ggtgagcttt tcggcagagt ctttgcggcg
     1801 cagttccagc cataaggact ccgcagtctc cccgccgccc atggtgaacg ccgccgtgac
     1861 ggcgcttttc tgcgagaccg tcgccgccgc gctttgttca ctgaaaccgt tatattgccg
     1921 cccggacatc accgccacat cggagacgat cgagtctttc caataaatct tgagcgtgac
     1981 cgtggcattg ttttccccgt ccgaaagctg gaacgtcacc tcgtccggat gttccttggc
     2041 ctgaaaaccc ggtgcatttt tcagcacgag ctctattacg gcctcgtagc ggctgtccgt
     2101 ggtccccagc gggacggcgg ttggcgcgcc ggcatgcaga tcataggcat cggtcatgag
     2161 gttttccgag ctccagcccg gaaactttat tggccgcagc gtcagagaga actgctcttc
     2221 cgtcggttcc gccgcggcgg atacgagatt ggccgtttga gaaatattcg acagcttcag
     2281 gttgcggctg tcgccggatg gaatgatcag cctgcccgta tagctgccgc tttgggtggc
     2341 gtaaagctct accgtttgat ccaccaaaga gcttcctttg gtgacgatcg ccacgttcac
     2401 cgtggttctc gccccgtctt cctcccgctt gagcacaagc tttatggtcc ccaagtcctt
     2461 gctgacggag atgttgtttg cataggtcaa ataaaagtca atgcgcggcg tgccgttgtt
     2521 ctgcgtctgc ggcgtttttt gcccaatgac ggtatttttt ccgccgcttt gcaccgactc
     2581 gtcggaaatc accttgccgg atacgtcctg cgtctgaaat ccggtgccaa tcctcataaa
     2641 aaggccaaag gtgtccagcg gggaatcgct gatctttttt tgttcctccg ccagttcaac
     2701 cgccgttgtg gtcaaagcgc cattgacttc ggcggcggtc acccacgcgg catcaaggcc
     2761 gtttttggct gcctccacca gggtgatccc gccggggaga acgacgcttt cgatgctgta
     2821 agcggtctcc ttgtccaccg gcggcagctc gatgcttccc gaggccacgg aataatcgct
     2881 tccatagccg ttatcgcccg cttttaaggt ctgggcggtc agcaccgtgt atcgatccgc
     2941 ggtggcgttc gcgcctttga cctgccagta gcggtactcc tcgcccgccg tgtgatacgc
     3001 cagctcttcg ctttcccccg gcgcttgaaa accgccgtct ccactgtttg ccccggtgtt
     3061 tacccgggca taggcatagg cttccgccgt gtcccctgcc agcatatacg catagcccga
     3121 caccgccccg tagactgttt tgcccgcgtc gtcggtgcag gcgacctgaa acaccacacc
     3181 ggcgttaaac cgtacggtgt tgggtgtggt ttggaggccc tcttttggta ccgggttgcc
     3241 gtctgcatcc gcacttttaa aatttgcggt ctcaatgacg gccgagtcca ggatcagcgt
     3301 gctgccgccc tgcaggacca cgccgtttcc attgccgtca atatcgcctt tgtccccgat
     3361 gcggttcagc gcgtatttcg cggtctggtt ggcgttggca gcgtcggatt gtccgctcaa
     3421 ctgaacatgc gtatcgatca aagtcacgcg gtcggccctc tggacggccg ccaaagtccg
     3481 cgtggcgccg gtcaccgttc cacctgtttc aatcggcctg ccgtagctgc gcaacgtcac
     3541 cagccgggtc gaacccgcgt cgcaggaagc gccgctgccg aacacgccgc cggtgacggt
     3601 catgcccggc ttgccggc
//
LOCUS       DBMDYA010000237         3595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5939_length_3595_cov_1.423583, whole genome shotgun sequence.
ACCESSION   DBMDYA010000237 DBMDYA010000000
VERSION     DBMDYA010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5939_length_3595_cov_1.423583"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="leuS"
                     /locus_tag="ACZPFK_06820"
     CDS             complement(<1..1269)
                     /gene="leuS"
                     /locus_tag="ACZPFK_06820"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1406057.1"
                     /translation="MKYDFKAIEAKWQKKWEDEHTFAAKQDFSLPKFYALVEFPYPSG
                     QGLHVGHPRSYTALDIVARKKRLDGYNVLYPMGWDAFGLPTENFAMKNHIHPEIVTKN
                     NVARFKSQLKSLGLSFDWDREVNTTDPSYYKWTQWIFLQLYKKGLAYKKEMSVNWCTG
                     CKVVLANEEVVNGTCERCHSPVVHKVKSQWMLRITAYADRLIDDLDTVNFIDRVTTQQ
                     KNWIGRSHGAEVTFGTTEGDDMVVYTTRPDTLFGVTYMVIAPEHKFVDKWADKITNIE
                     AVRAYREAAARKSDFERTELAKDKTGVQLQGVMAINPVNGQQIPIFISDYVLATYGTG
                     AIMAVPGHDQRDYDFAKAFGLPIVEVVAGGDISKEAFADCATGVMVNSGFLTGMTVDE
                     AKAAITEWLEKEGKGVKKTNFKLRDWVFSRP"
     gene            complement(1351..1695)
                     /gene="rsfS"
                     /locus_tag="ACZPFK_06825"
     CDS             complement(1351..1695)
                     /gene="rsfS"
                     /locus_tag="ACZPFK_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1406058.1"
                     /translation="MTSLELATVAAKAMDSKKAKDIRVLKVEDLTVLTDYFVIGTGSS
                     ATQVKALADEVEYQLGQAGVKPLRREGMDARNWILLDYGTVIVHVFYPETRDFYDLEH
                     LWADATPVEIEF"
     gene            complement(1744..2973)
                     /locus_tag="ACZPFK_06830"
     CDS             complement(1744..2973)
                     /locus_tag="ACZPFK_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1406059.1"
                     /translation="MAKKPTNPYYHPEEQRRSAPQRAASPYSDHRAASPAQQRAAAAR
                     RKKAARAKRRKRRLAVFAAVLLASAVLCVAVYNLLNNLVTGGGGVLPDEIGTEDALKG
                     DVVNILCAGIDFEEGREYPNGMGFTDVILYVSFDMKANKISMLQIPRDTYIGETTTTG
                     KINAVALQGKSDPPIAALATLVNQQFKLPVDYYVTIDMDAFKAIVDAIGGIEVTVPFD
                     ITDDYGNTLHAGTQVVDGATAEFMVRQRHNYGNADIGRLEMQRYMYSALLKTFKSFPV
                     SDILKVMPAYIQYVKTDINLAKMGSLATKVVKVPSENIVMFKVPGEACTVGSQSVFTV
                     HLEATANILNSYFRPYSDPVPAAELGLIELQNTVPDVDGGTGQTMDSIDSGTAQWGQG
                     NVDPNAAAGDGSSAPAA"
     gene            complement(3008..>3595)
                     /gene="yqeK"
                     /locus_tag="ACZPFK_06835"
     CDS             complement(3008..>3595)
                     /gene="yqeK"
                     /locus_tag="ACZPFK_06835"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR1406060.1"
                     /translation="GSAKMVTAEFCKKLAKRNLSAKRYQHTLNVRKLAVKLAKRYGAD
                     EDKAEIAALLHDICKERPKGELLQMVRDNAIMTQNAAEKPQGVWHAAAAMVVARQELG
                     VEDGEILNAIRYHTSGRAGMSLLEKVIYMADMCSEERGYPEAEELRKLLKKDMDKALL
                     RALQYSLQWLREEHKPIDEDSLFALHWLEEKYHRA"
BASE COUNT          568 a         1113 c         1095 g          819 t
ORIGIN      
        1 cgggcggctg aacacccagt cgcgcagctt gaagttggtt tttttgaccc ccttgccctc
       61 tttttccagc cattcggtga tggccgcctt ggcctcgtcc accgtcatgc cggtcaaaaa
      121 gccgctgttc accatcacgc cggtggcgca gtcggcaaag gcctccttgg agatatcgcc
      181 gcctgcgacc acctccacga tgggcaggcc gaacgccttg gcaaagtcgt agtcgcgctg
      241 gtcatggccg ggcacggcca tgatggcgcc ggtgccgtag gtggccagca cgtaatcgga
      301 gatgaaaatg gggatctgct gcccgttcac cgggttgatc gccatgaccc cctgcagctg
      361 cacgccggtc ttgtcctttg ccagctcggt gcgctcaaaa tcgctcttgc gggcagctgc
      421 ctcgcgatag gcgcgcaccg cctcgatgtt ggtgatcttg tcggcccact tgtccacaaa
      481 cttgtgctcg ggcgcgatga ccatgtaggt gacgccgaac agggtgtcgg ggcgggtggt
      541 atagacgacc atatcgtcgc cctcggtggt gccgaaagtg acctcggcgc cgtggctgcg
      601 gccgatccag ttcttctgct gggtggtgac gcggtcgatg aaattgactg tgtccaggtc
      661 gtcgatcagc ctgtcggcat aggcggtgat gcgcagcatc cactggcttt tcaccttgtg
      721 caccaccggg ctgtggcagc gctcgcaggt gccgttgacg acctcctcgt tggccagcac
      781 caccttgcag ccggtgcacc agttgacgct catctctttt ttgtaagcca gtcccttttt
      841 gtacagctgt aaaaagatcc actgggtcca cttgtagtag ctggggtcgg tggtgttgac
      901 ctcgcggtcc cagtcaaagg agaggcccag gcttttgagc tggcttttga aacgggccac
      961 gttgtttttg gtgacgatct cggggtggat gtggtttttc atcgcaaagt tctcggtggg
     1021 caggccaaaa gcgtcccatc ccatgggata gaggacgttg tacccgtcca gccgcttttt
     1081 gcgggccacg atgtccagcg cggtgtagct gcgcggatgg cccacatgca atccctggcc
     1141 cgagggatag ggaaactcca ccagggcgta gaacttgggc aggctgaaat cctgcttggc
     1201 cgcaaaggtg tgctcgtcct cccacttttt ttgccatttc gcttcaatgg ccttgaaatc
     1261 gtacttcaca actcattcct cccacactgt ttgccgcccg ggccgggcgg ctcctctcaa
     1321 aaacgcccgc cgcggggtga aaagaaagcc ttaaaactcg atctccaccg gggtcgcgtc
     1381 ggcccacagg tgttccagat cataaaaatc ccgcgtctcc gggtagaaga cgtgtacgat
     1441 gacggtgccg tagtccagca ggatccagtt gcgggcgtcc atgccctcgc ggcgcagcgg
     1501 tttcacgccc gcctggccca actggtattc cacctcgtcc gccagcgcct tgacctgggt
     1561 ggcgctggag ccggtgccga tgacaaaata atccgtgagg acggtcaggt cctccacttt
     1621 cagcacgcgg atgtcttttg cttttttcga atccatcgcc tttgcggcga cggttgcaag
     1681 ctctagggat gtcattgctt tcccgccttt cttttttctc atgatcggcc tgccggcggc
     1741 tgtttatgcc gccggtgcgg aggagccgtc ccccgccgcc gcattcgggt ccacattgcc
     1801 ctggccccac tgggcggtgc cgctgtcgat gctgtccatg gtctggccgg tgccgccgtc
     1861 cacgtccggc accgtgttct gcagctcgat gaggcccagc tctgcggcgg gcaccggatc
     1921 ggaatagggc ctgaaatagc tgttcaggat gttggccgtc gcctccagat gcacggtaaa
     1981 tacgctctgg ctgcccacgg tgcaggcctc gcccggcacc ttgaacatga cgatgttttc
     2041 gctgggcacc ttgaccacct tggtggcgag cgagcccatc tttgccaggt tgatgtcggt
     2101 cttcacgtac tggatatagg cgggcatcac cttgaggatg tccgagacgg ggaagctttt
     2161 aaaggtcttg agcagcgccg aatacatata gcgctgcatc tccagccgac cgatgtctgc
     2221 gttgccgtag ttgtgccgct ggcgcaccat gaactcggcg gtggccccgt cgaccacctg
     2281 ggtgccggcg tgcagcgtgt tgccgtaatc gtcggtgata tcgaaaggca ccgtcacctc
     2341 gatgccgccg atggcgtcga cgatggcctt gaacgcgtcc atgtcgatgg tgacatagta
     2401 gtcgaccggc agcttgaact gctggttgac cagggtggcc agggccgcga tgggcgggtc
     2461 gctcttcccc tgcagggcca cggcgttgat cttgccggtg gtggtcgtct cgccgatata
     2521 ggtgtcccgc gggatctgca gcatcgagat cttgttcgct ttcatgtcga agctgacata
     2581 gaggatcacg tcggtgaagc ccatgccgtt cgggtattcc cgtccctcct caaaatcgat
     2641 gcccgcgcag aggatgttga ccacatcccc cttgagggcg tcctcggtgc cgatctcgtc
     2701 gggcagcacg ccgccgcccc cggtgaccag gttgttcagc agattgtaga ccgccacgca
     2761 gagcaccgcc gaggccagca gcacggcggc gaaaacggcc agccgccttt tgcggcgctt
     2821 tgccctcgcc gcctttttgc ggcgggcggc cgcagcgcgc tgctgggccg ggcttgccgc
     2881 ccggtggtcg ctgtaggggg aggccgcccg ctgcggggcg ctgcggcgct gctcctcggg
     2941 atgataatag gggttcgtag gtttttttgc catagtttca ggttgccttt cctgggtctc
     3001 aaacgcgtca ggcgcggtga tatttttctt ccagccagtg cagggcgaac agcgagtcct
     3061 cgtcgatggg cttgtgctcc tcccgcagcc actgcaggct gtactgcagc gcccgcagca
     3121 gcgctttgtc catatccttt ttcagcagct tgcgcagctc ttccgcctcc gggtagccgc
     3181 gctcctcgct gcacatatcc gccatataga tcactttttc cagcagggac atacccgccc
     3241 ggccggaggt gtggtagcgg atggcgttta agatctcgcc gtcctcgacg cccagctcct
     3301 gccgggcgac caccatcgcg gcggccgcat gccagacgcc ctggggcttt tcggccgcat
     3361 tttgtgtcat tatagcatta tcgcgcacca tctgcaacaa ttcgcccttg gggcgctctt
     3421 tgcagatatc gtgcagcagg gccgcgatct ccgccttatc ctcgtctgcg ccatagcgtt
     3481 ttgccagctt gaccgcgagc ttgcgcacgt tcagcgtgtg ctggtagcgc ttggcgctga
     3541 ggttgcgctt ggcaagcttt ttgcagaact ccgcagtgac catttttgca cttcc
//
LOCUS       DBMDYA010000238         3593 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5942_length_3593_cov_1.555842, whole genome shotgun sequence.
ACCESSION   DBMDYA010000238 DBMDYA010000000
VERSION     DBMDYA010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_3593_cov_1.555842"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..1675)
                     /locus_tag="ACZPFK_06840"
     CDS             complement(74..1675)
                     /locus_tag="ACZPFK_06840"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1406061.1"
                     /translation="MEKDILIQNIREGNTVLGLELGSTRIKAVLIGPDHAPLVSGSHD
                     WENRLEEGYWTYHLEDVWAGIQDAYANLAAAVKKEYGIPLCTVKAMGFSAMMHGYLPF
                     DRDGRQLAAFRTWRNTTTGGAAAELTEIFGFNIPQRWSVAHLYQAILNGEEHVKELSF
                     FTTLAGYVHWRLTGEKVLGVGEASGMFPIDSKTCDFDAGMKAKFNTLLKERGLPFCLD
                     DILSRVLPAGVPAGRLTPEGARLLDPSGCLQPGISLCPPEGDAGTGMTATNSVAQRTG
                     NVSAGTSVFAMVVLERPLSRVYPEIDMVTTPTGRPVAMVHCNTCTSDLDAWVKMLGEM
                     AGASGAVLSKPQLYDLFYSKALEGEPDCGGLVNINYYSGEPVTGFEEGRPLFLRRPGA
                     RLTLANFARAQLYSTIATLKLGMGLLLEQEHVQLETLLGHGGLFKTAEVGQRLLAGAL
                     RVPVAVMETAGEGGPWGMALLASYMAEREQAETLESYLASKVFAKTSCNVQQPHDDDT
                     AGFARFMENYKSALAVERAAVAALK"
     gene            complement(1955..3094)
                     /locus_tag="ACZPFK_06845"
     CDS             complement(1955..3094)
                     /locus_tag="ACZPFK_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1406062.1"
                     /translation="MFEFFTLYDTIIINFKQNWRNSKTFMEKYKKYALLAEKLSEEIC
                     MGKYAVGERLLSEKELAASAGLSRQTVRQALSLLEQQRLIDRRQGSGSRVLGPSSFQP
                     RQERSYNIAVITTYIGEYIFPDILRGIEKVLMSHQYSPLISATHNRVDNERKILLDCL
                     DRPIDGLIVEGTKTALPNPNIDLYQKLSDRSIPVVFVHGFYPDLSHPVFVVSDDRKGG
                     QQATELLIKKGHTRIGGIFKSDDIQGHRRYAGYTQALRDAGLELADNRVLWYTTENRS
                     SLLSSYALTTLADCSAVVCYNDEVALQMIDLLRTAHRRIPEDVALISFDNSHYCSLSP
                     VPITSFSCQKRQIGVLAAQKLLRLIDGVQQQSTVLPWKLIERASC"
     gene            3402..>3593
                     /locus_tag="ACZPFK_06850"
     CDS             3402..>3593
                     /locus_tag="ACZPFK_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406063.1"
                     /translation="MGQHKYALIATRIREEIQTGEYHPGDRLPTEFDLTKRFQVSRQT
                     VRQAMAQLQKEGYLIQRQGS"
BASE COUNT          935 a          905 c          925 g          828 t
ORIGIN      
        1 aaaacaattt atctctaagg ccccggccag aggacgggcc ggatagccac aaaaaatagc
       61 ctgcggctat tttttatttc agtgccgcca ccgcggctct ctccacggca agagcgctct
      121 tgtaattttc cataaagcgg gcaaagccgg cggtatcgtc gtcatggggc tgttgcacat
      181 tacagcttgt cttggcaaag accttggacg caagataaga ttccagagtt tccgcttgct
      241 cccgctcagc catgtaggaa gccaacagcg ccatccccca gggccctccc tcgcccgcgg
      301 tctccatgac tgccacgggc actctgagcg caccggccaa aagccgctgt cccacctccg
      361 cagtcttgaa caatccgccg tgccccagca aggtttccag ctgcacatgt tcctgttcaa
      421 gcagcagacc catgcccaat ttgagggtgg ctatagtgga gtaaagctgc gcgcgggcaa
      481 agttcgccag cgtgagccgg gcgcccgggc gtcgcagaaa gagcggtctt ccctcttcaa
      541 atccggtgac cggctcgcct gagtaatagt tgatgttgac aagcccgccg cagtccggct
      601 ccccctccag cgccttgctg taaaagaggt cgtagagctg aggctttgag agcacagcgc
      661 cggatgcgcc cgccatctcc cccagcatct ttacccaggc gtccagatcg gaagtacagg
      721 tattgcagtg aaccatcgcc accggcctgc cggtgggagt ggtcaccata tcaatctccg
      781 ggtagacccg ggaaaggggg cgctccagta cgaccatcgc aaagacggag gtaccggccg
      841 atacgttgcc ggtgcgctgt gccaccgagt tggtggcggt catgccggtg ccggcgtccc
      901 cctcgggcgg gcagagggaa atgccgggct gcaggcagcc gctggggtcc agcaggcgcg
      961 cgccctccgg ggtcaaccgc ccagccggca ccccggccgg cagcacccgc gacaatatgt
     1021 catccagaca aaaaggcagc ccccgctcct ttaacagagt gttgaatttt gctttcatcc
     1081 ccgcgtcaaa gtcacaggtt ttgctgtcga tggggaacat cccggaggct tctcccacgc
     1141 caagcacctt ttcgccggtc agccgccagt gaacatagcc tgccagggtg gtgaaaaaag
     1201 acagctcttt tacatgctcc tcgccgttca ggatcgcctg atacagatgt gcgacgctcc
     1261 agcgctgggg aatgttgaag ccgaatatct cggtcagctc ggcagccgcc ccgccggtgg
     1321 tggtgttgcg ccaggtacgg aatgccgcca gttgacgccc atcgcggtca aaggggagat
     1381 aaccgtgcat catcgccgag aagcccattg cttttacagt gcaaagcggg atgccgtatt
     1441 ctttttttac agccgcagcg aggttggcgt aggcgtcttg aataccggcc cagacatcct
     1501 ccagatgata ggtccagtag ccctcttcca ggcggttttc ccagtcatgg ctgccagaga
     1561 ccagcggtgc gtggtccgga ccgatcaaaa cagccttgat gcgggtggag cccagctcta
     1621 aaccaaggac tgtgttcccc tctctgatgt tctgaataag aatatctttc tccatcttct
     1681 cactgccttt ccagggccag cccaaaccag catggagttc aaaatagttc aacaagataa
     1741 aaattatgct gtatttgtat ggtctgacgt caaaaatagt acaaaaataa aaaaataaaa
     1801 attatttttt aaaaattgta cgtacaactt ttattaaagt ttaccgcact tctcccgatt
     1861 tggcaatatc aactattact aaaaaaataa aacccttatt tagcaatatt gccagagaag
     1921 tgcggcggag aaaagaaaat acactcagaa aggatcagca gctggcacgt tcgatgagct
     1981 tccagggcag cacagtggac tgctgctgca ccccgtcgat caggcgcagc aatttttggg
     2041 cggcgagtac cccgatttgt cgtttttgac aagaaaaaga agtgatgggc acaggggata
     2101 aagaacaata atggctatta tcaaagctga tcagtgcaac atcttctgga attctgcgat
     2161 gagctgtgcg caaaaggtcg atcatctgca aggccacctc gtcattataa cagactactg
     2221 cgctgcaatc ggctagagtc gtcagcgcat aagaggagag cagagaagaa cgattttcgg
     2281 tggtgtacca cagcacacga ttatcggcta gttcgagtcc cgcatcacgc agggcttggg
     2341 tatagcccgc gtaacggcga tgcccttgaa tgtcgtcgct tttaaatatg ccgccgatgc
     2401 gtgtgtggcc ttttttaatg agcagctcgg tcgcctgctg accacccttg cggtcgtcgc
     2461 tgaccacaaa gacgggatga gaaaggtcgg gatagaaacc gtggacaaaa acaacgggaa
     2521 tgctgcggtc gcttagtttt tgataaaggt caatattggg gttaggtaaa gcggttttag
     2581 tgccttctac gatcaaaccg tcgatagggc ggtccaggca gtccaagagt atctttcgtt
     2641 cattgtcgac gcgattgtgg gtggcggaga taagcggaga atattgatgg gacatcaaaa
     2701 ccttttcaat tccacgcagg atatctggaa agatatattc tccgatgtag gtggtgatga
     2761 ccgcaatgtt atagctgcgc tcttggcggg gttgaaaaga agaggggccc aatacacgag
     2821 aaccgctgcc ctgcctgcgg tcgatgagcc tttgctgctc taaaagagaa agtgcctgac
     2881 gcaccgtttg cctgctaagc cctgcagaag ctgcaagctc cttttccgaa aggagccgtt
     2941 ctcctaccgc gtattttccc atacaaattt cttcggaaag tttttcagct aaaagagcat
     3001 attttttata tttttccata aacgtcttgc tatttctcca attttgttta aaatttataa
     3061 taattgtatc atacaaagtg aagaattcaa acaacttttc tttctcatga agcagagcag
     3121 aaaaaaggaa aaatcaggaa ataaaatcca taattttaga agattgttgt tgaaataaaa
     3181 agggccttaa gattttttga catataggat agtatcgggt aaaaatttgt gaaaagataa
     3241 ttcaataaaa tgggattttc ataaaaaaag aaatcttgaa ttgtgaggtc aatcggcaat
     3301 acttgtattt taatgggaaa aattgtaaaa tgtaaaatga tatgacttat taataagctc
     3361 ttctataaaa attccattgt acagaaatgc aggtgaacga gatgggacag cataaatacg
     3421 cgttgattgc gacccggatc cgggaagaaa tccagacggg agaatatcac ccgggagacc
     3481 gtctccccac cgagtttgat ttgacaaagc gttttcaagt cagccggcag accgtccggc
     3541 aggctatggc ccagttgcaa aaagagggat accttatcca gaggcagggc agt
//
LOCUS       DBMDYA010000239         3586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5956_length_3586_cov_1.612626, whole genome shotgun sequence.
ACCESSION   DBMDYA010000239 DBMDYA010000000
VERSION     DBMDYA010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5956_length_3586_cov_1.612626"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(64..1413)
                     /locus_tag="ACZPFK_06855"
     CDS             complement(64..1413)
                     /locus_tag="ACZPFK_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1406064.1"
                     /translation="MSDKLHSLEYLCEEYLKNNYIDPEMSEKLGVKRGLRNPDGTGVL
                     AGLTNVCDVVGYDMIDGVRTPRDGQLIYRGIDVQDIVKAAYAEDRFIFEEVIWLLLFG
                     SLPTAAELETFKDTMEESRGLPENFVEDMIIKAPSPNIMNKMQRSVLALYSYDDDPES
                     QTLKNILRQSVVLISSLPTIMVNAYQVKRRVYDKKSMYFHMPKAGQSTAEHILRTYRA
                     DRKFTHEEAKLLDLCMVLHADHGGGNNSTFACRVLSSTGTDTYSAIAAGIGSLKGPKH
                     GGANIKVMEMLDDIKANVGDYDDDTEIKEYLRRLIRKQAGDRSGLIYGIGHAVYTKSD
                     PRAVILRTNAEHLAYEQGFGDDFKLLCAVERLAPQVFAEEKGNKNVCANVDLYSGLIY
                     RMLGLSVDLYTPLFAISRIPGWCAHRIEEVVFANRIMRPAYKYLGVRQHYISLEDRR"
     gene            1579..2634
                     /gene="spoIID"
                     /locus_tag="ACZPFK_06860"
     CDS             1579..2634
                     /gene="spoIID"
                     /locus_tag="ACZPFK_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HIR1406065.1"
                     /translation="MRKLFSSLLLFAALTLLLPIAAAALSGGGLPQVDAFTAMLSKPG
                     AGAGASSAASQAGQGDADAAWASQALPILNSATGEVETVSLRDFLIGAAASEMPISYQ
                     KEAFKAQIVAAHSYALANRDAQLSAPDPALNGAWFKANPAGYEGYIKPESLKTLWGDK
                     YDANYAYLSALVDEVGAQVLTYDGAAALTTYYAISNGKTESSEAVWGTALPYLSTVDS
                     PLDLTSPDYEQTVTLSSQDVYDRLNLSFLALDLSGAPNTWFGEAVRDEAGYIATMPVG
                     GQTLKGTELRAALGLRSADFDVSVDAENQFTFTTRGYGHGAGLSQYGANAMAITGKTY
                     AEILAYYYPGTALGAAG"
     gene            complement(2905..3426)
                     /gene="dcd"
                     /locus_tag="ACZPFK_06865"
     CDS             complement(2905..3426)
                     /gene="dcd"
                     /locus_tag="ACZPFK_06865"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR1406066.1"
                     /translation="MILSGKEIHKHIGGKIVIEPFDEKKLNPNSYNLTLADELLVYDQ
                     PVLDMKRPNPTSRLTIPEEGMVLQPHRLYLGRTEEYTRTEGYVPMLEGRSSTGRLGLF
                     IHVTAGFGDVGFAGYWTLEIFCVQPIRIYPHVEICQIYYHDIDGEYEPYRSGKYQHNT
                     GIQPSLMYRDFEK"
BASE COUNT          620 a         1174 c         1117 g          675 t
ORIGIN      
        1 cgtaatcagc aaaaaagacg ccgccggacg caaggcagag cagcgcgcag aaaacacaca
       61 ggctcatcgg cgatcctcca ggctgatata gtgctggcgc acgcccaggt atttgtaagc
      121 gggccgcatg atgcggttgg caaagacgac ctcctcgatg cggtgggcac accagccggg
      181 gatgcggctg atggcgaaca gcggggtgta cagatccacc gacaggccca gcatgcggta
      241 gatcaggccg ctgtacaggt ccacgttggc gcagacgttc ttgttgccct tttcctccgc
      301 aaagacctgg ggcgccagcc gctccaccgc gcacagcagc ttgaaatcgt ccccaaagcc
      361 ctgctcgtag gccagatgct cggcattggt gcgcaggatg accgcgcgcg ggtcgctctt
      421 tgtatacacc gcgtgcccga tgccgtagat caggccgctg cggtcgcccg cctgcttgcg
      481 gatcaagcgg cgcaggtact ccttgatttc ggtgtcgtcg tcatagtcgc ccacgttcgc
      541 cttgatgtcg tccagcatct ccatgacctt gatgttggcg ccgccgtgtt tggggccttt
      601 caggctgccg atgcccgcgg cgatggccga ataggtgtcg gtgccggtgg aggacagcac
      661 gcggcaggca aaggtggagt tgttgccgcc gccgtggtcg gcgtgcagca ccatgcacag
      721 gtccagcagc tttgcctcct cgtgggtgaa cttgcggtcc gcccggtagg tgcgcaggat
      781 gtgctcggca gtgctctggc cggccttggg catgtggaaa tacatgctct ttttgtcgta
      841 gacgcggcgc ttgacctgat aggcgttgac catgatggtg ggcagagacg agatgagcac
      901 caccgactgg cgcagaatgt ttttcagcgt ctggctctca gggtcgtcgt catagctgta
      961 cagcgccagc acactgcgct gcatcttgtt catgatgttg ggcgagggcg ccttgatgat
     1021 catgtcctcg acgaaatttt ccggcaggcc gcggctctcc tccatcgtat ccttgaaagt
     1081 ttccagctcc gcggcggtgg gcaggctgcc gaacagcagc agccagatga cctcctcaaa
     1141 gatgaagcgg tcctcggcgt aggccgcctt gacgatgtcc tgcacgtcga tgccgcggta
     1201 gatcagctgg ccgtcgcgcg gggtgcgcac cccgtcgatc atgtcgtagc ccacgacgtc
     1261 gcagacgttg gtcaggcccg ccagcacacc cgtcccgtcc gggttgcgca ggccgcgctt
     1321 gacgccgagc ttttcagaca tctcggggtc gatatagtta ttttttaagt actcctcaca
     1381 tagatattcc aaggaatgca gtttgtccga cacattacca ccctctctgt cggcgcgtcg
     1441 tttttaccgc gcccgttata cctccctatc ataatccaaa cggcggcgat gtgcaagcat
     1501 tccctgtcgg cggccacggt gcaaaacgcg gctgcagcgg cctcccgaaa gcccgtcaaa
     1561 agaaaggact gctgaaaaat gagaaagctt ttttcttccc tgctgctctt tgcggcgctc
     1621 acgctgctgc tgcccatcgc cgcggcggcg ctctccggcg gcggactgcc ccaggtcgac
     1681 gctttcaccg ccatgctctc aaagcccggc gcgggcgcag gggcctcctc cgccgcctca
     1741 caggccgggc agggggatgc ggacgcggct tgggcgtccc aggccctgcc catcctcaac
     1801 agcgccaccg gggaggtgga gacggtttcg ctgcgtgact tcctcatcgg cgcggcggcc
     1861 agcgagatgc ccatcagcta tcagaaagag gccttcaagg cgcagatcgt cgccgcccac
     1921 agctacgccc tggccaaccg cgacgcgcag ctgtccgccc cggacccggc gctgaacggc
     1981 gcctggttca aggcgaaccc cgcgggctat gagggctaca tcaagccgga gagcctcaag
     2041 accctgtggg gggacaaata cgacgccaac tacgcctatc tctccgctct ggtggacgag
     2101 gtgggcgcgc aggtcctcac ctacgacggc gcggcggcgc tgaccaccta ttacgccatc
     2161 tccaacggca aaaccgagag cagcgaggcg gtgtggggca ccgccctgcc ctatctgtcc
     2221 accgtcgact ccccgctgga cctgaccagc ccggactatg aacagacggt caccctgtcc
     2281 tcacaggacg tctacgaccg gctcaacctc agctttctgg cactggacct ctcgggcgcg
     2341 ccgaacacct ggtttgggga ggccgtgcgg gacgaggcgg gctatatcgc caccatgccg
     2401 gtgggcggcc agacgctgaa gggcaccgag ctgcgggccg ccctggggct gcgcagcgcg
     2461 gacttcgacg tttcggtgga cgcggagaac cagttcacct ttaccacccg cggctacggc
     2521 cacggcgccg ggctctccca gtatggggcc aacgccatgg ccatcaccgg caagacctac
     2581 gccgagatcc tggcctacta ctacccgggc acggcactgg gggccgcggg ctgacgccat
     2641 catcaaactg cgggcaggga gacaatcccc tgcccgcagt tttttatctt gacggccacg
     2701 cctgccgccg cacggccccc ggccgctctt ccggccgggc gcagggacca aagcgcccgc
     2761 agaaagcggc ggcaaaagag ggcggcggac ccccggctta ttttcttttt gccgcaagct
     2821 gctgccaaca aaccggcgcg gattaaactt tccgccgctt cctggcggtt cccttggccg
     2881 ggggagccgt gcggctccca ccgctcactt ttcaaaatcc cgatacatca gactgggctg
     2941 gatgccggtg ttgtgctggt acttgccgct gcggtagggc tcgtactcgc cgtcgatatc
     3001 gtgataatag atctggcaga tctccacatg cgggtagatg cggatgggct ggacgcagaa
     3061 gatctccagc gtccagtagc cggcgaagcc cacatcccca aagccggccg tcacatggat
     3121 gaacaagccc aggcggccgg tggacgagcg cccctccagc atcggcacat acccctcggt
     3181 tcttgtatat tcctcggtgc ggcccagata cagccggtgg ggctgcagca ccatgccctc
     3241 ctcggggatg gtcaggcggc tggtgggatt cggccgcttc atgtccagta ccggctggtc
     3301 gtagaccagc agctcgtccg ccagcgtcag gttgtagctg ttggggttga gttttttctc
     3361 gtcaaagggc tcgatgacga tcttcccgcc gatgtgctta tggatctctt tgcccgacag
     3421 gatcatggca tttccactcc tctttttcgt attggcgcgg cgggaccgcc ccgcgccgtc
     3481 tcatccgcct ccaccgccgc ccgcgcgtcc gccacgaggc cggccgggaa gcggtaatgg
     3541 tccagcagcc ggatggcgtt gcgggtgcgg caggggccgt ccttga
//
LOCUS       DBMDYA010000240         3583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5965_length_3583_cov_1.599368, whole genome shotgun sequence.
ACCESSION   DBMDYA010000240 DBMDYA010000000
VERSION     DBMDYA010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5965_length_3583_cov_1.599368"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="ACZPFK_06870"
     CDS             complement(<1..871)
                     /locus_tag="ACZPFK_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1406067.1"
                     /translation="MKKLVALFLAAALSLSLAACGGGASSSTPAGSGSGAASTSGTAS
                     TPKEPLKYVFLYGGSLGGQAFADVVDAGLKKAAADFGAEYSVLEKVEGSAAADTFRTV
                     IESGVDLIIIADVKWNEALAEVADDYPDFPFVHMDADGTGALSAEHPNIYEVSYKEHE
                     SAFLNGVFCALMSKTGKVAQIQGGDSGTMIRFNSGFRAGVQYVLDTDPSTSVVGFSDV
                     NKGYETAMLYYDQGYDWLAAVAAGSNLGVFQASQEKGGDNWCCGAADGQFHLMPDRIV
                     CSQVKTIDHVGYNY"
     gene            complement(928..1947)
                     /locus_tag="ACZPFK_06875"
     CDS             complement(928..1947)
                     /locus_tag="ACZPFK_06875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406068.1"
                     /translation="MEKKIPVIIDCDTGVDDAGALMMACASDRLDILAVTTVSGNVGL
                     EHTHPNTLNVLKLFGRGEIPVAKGADAPLVRPPFKASAVHGFNGLRNYQFAENTTAAQ
                     VDLPAAAYMAKLLEERAEKITIIALGPLTNVAILLLARPDLADKIDKIIFMGASYQDG
                     NPTPIGTFNVLVDPEAFRICTTRGVDFYACPLDTLRNACVTQKDVERVRGFKSAAADF
                     IYRTAFAPRTYTDEEIARINASGNEELVTRERLERMNRSEKFALLDQATMAFAIAPEL
                     FTYKKYYCTVECAGTFTAGYTYVDKRNYYKKSEEEKNLFFVETVDGEKFASLFMSLLE
                     SYETK"
     gene            2302..3300
                     /locus_tag="ACZPFK_06880"
     CDS             2302..3300
                     /locus_tag="ACZPFK_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406069.1"
                     /translation="MTLKEIAALAGVSVSTVSRVINNQNTKCASQEVQNKIWAAVRSL
                     GYTPNPHAQSLKRNDLEKSSRSFHSSIAYACSEQFLQGSHHDWEMLSLIEKEILASHY
                     SVSAPRADLPAAEEKVRDLGLVLVGAESERRIAQYIEKYKNVVCISEHHTGQPCDLVL
                     LDMRKSLLRAWEYLSELGHRRIAFAGAEKGDFFRTYTDVIPADAGAQFIPCGLSMSDG
                     MLLAGELCDCLMVPSALVCENNSIACGAIQALREHRLAVPGDVSVVSLGGGLDLSRAA
                     LPVSVTVFSYNKAEISRLAVDLLIDRIHRRHLLAVTYEPGCEMADNGSCAPAREVT"
     gene            3302..>3583
                     /locus_tag="ACZPFK_06885"
     CDS             3302..>3583
                     /locus_tag="ACZPFK_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012576.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406070.1"
                     /translation="MAFGIREIARLAGVSPATVSRILNDPAYRCSSEERRARVLEIAR
                     ANNYQPNEDAKRLRSGGSFSAPQKKICCFLARTLTSAPNGDQFFSSLARA"
BASE COUNT          650 a         1122 c         1097 g          714 t
ORIGIN      
        1 tgtagttgta gcccacgtga tcgatggtct tgacctgcga gcagacgatg cggtccggca
       61 tcagatggaa ctggccgtcg gcagcgccgc agcaccagtt gtcgccgccc ttttcctgcg
      121 aggcctggaa gacgcccagg ttggagccgg cagccaccgc agccagccag tcgtagccct
      181 ggtcatagta cagcatggcg gtctcgtagc ccttgttgac gtccgagaaa ccgacaacgg
      241 aggtggaggg atcggtgtcg agcacgtact ggacgccggc gcggaagccg ctgttgaagc
      301 ggatcatggt gccgctgtcg ccgccctgga tctgggcgac cttgccggtc ttggacatca
      361 gcgcgcagaa cacgccgttc aggaaagcgg actcatgctc tttataggac acctcgtaga
      421 tgttggggtg ctcggcggag agggcgccgg tgccgtccgc atccatgtgc acaaacggga
      481 aatcggggta atcgtctgcc acctcggcca gcgcctcgtt ccacttgacg tcggcaatga
      541 tgatcagatc cacaccgctc tcgatcaccg tgcggaaagt gtcggccgcg gccgagccct
      601 cgaccttttc cagcaccgag tactcggcgc cgaagtcagc cgccgccttt ttgaggccgg
      661 cgtccacaac gtcggcaaac gcctggccgc ccagggaacc gccgtacagg aaaacgtact
      721 tcagcggctc cttaggggtg ctggcggtgc cggaggtcga cgccgcgccg gagccgctgc
      781 ccgccggggt gctgctgctg gctccgccgc cgcaggcggc cagagagagg gaaagtgctg
      841 ctgccaggaa aagtgcaact aattttttca ttttttcctc cttggaactt ctcttttact
      901 ttattttagt ccgctcagcg gaccggatca cttggtctcg tagctctcga gcagggacat
      961 aaagagggat gcaaacttct cgccgtccac cgtctccacg aagaagaggt ttttctcctc
     1021 ctcgctcttt ttgtagtagt tccgcttgtc cacataggtg tagcccgcgg taaaggtgcc
     1081 cgcgcactcc acggtgcagt aatacttttt ataggtaaac agctccgggg cgatggcgaa
     1141 cgccatggtg gcctggtcca gcagcgcgaa cttttcgctg cggttcatcc gctccagccg
     1201 ctcgcgggtg accagctcct cgttgccgct ggcgttgatg cgcgcgatct cctcgtcggt
     1261 ataggtgcgg ggcgcaaagg cggtgcggta gatgaagtca gccgccgcgc tcttgaagcc
     1321 gcgcacccgc tccacatcct tttgggtcac gcaggcattg cgcagcgtgt ccagcgggca
     1381 ggcgtaaaaa tcgacgcccc gggtggtgca gatgcggaac gcctccgggt ccaccagcac
     1441 gttgaacgtg ccgatcggcg tggggttgcc gtcctgatag gatgccccca taaagatgat
     1501 cttatcgatt ttgtccgcca ggtccgggcg ggccagcagc aggatcgcca cattggtcag
     1561 ggggcccagg gcgatgatgg tgatcttttc ggcgcgctct tccagcagct tggccatata
     1621 agccgcggcg ggcaggtcca cctgcgcggc ggtggtgttc tcggcaaact gatagttgcg
     1681 cagcccgttg aagccgtgca ccgcgctcgc cttgaaaggc ggtcgcacca ggggagcgtc
     1741 tgcgcccttt gccaccggga tctccccgcg gccaaacagc tttaatacat ttaaggtatt
     1801 gggatgcgtg tgctccagcc ccacgttgcc ggaaaccgtg gtcaccgcca ggatgtccag
     1861 cctgtcgctg gcgcaagcca tcatcagcgc ccccgcgtcg tccacgccgg tatcgcagtc
     1921 gatgatgacc gggatcttct tttccatcgc atttcctcct ttttgaacgt ctttcctccc
     1981 gaggcgccgc cggagggagg gggaggcgtc cgctgcagcc ctccggtcga tctacttaaa
     2041 ttatataaca gctttcccca aagtcacagc catttttagc aatcgttttc aatccaaatt
     2101 tttgtatttc ttgtacaatg taacaacaat ttttctgtaa gattcgctgt tccaatgtgg
     2161 gttattgcca aaaaactcct gccgtaaaat tgatttttat ggaatatttg atttcgcgtt
     2221 ttttaaatgg tataatttag tcaatcgatt tcacatccat tttatccgcg ccgctacccg
     2281 gcggggaggg gagggactga aatgacgctc aaagagatcg cggcgctggc gggggtctcc
     2341 gtctccacgg tgtcccgggt catcaacaac cagaacacca aatgcgcctc gcaggaggtg
     2401 cagaacaaga tctgggccgc ggtgcgcagc ctgggctaca ccccgaaccc ccacgcccag
     2461 agcctgaagc ggaacgacct ggaaaaatcc tcccgctcgt ttcacagctc catcgcctat
     2521 gcctgctcag agcaattcct gcagggcagc caccacgact gggagatgct ctcgctgatc
     2581 gaaaaggaga tcctcgccag ccactactcg gtgagcgcgc cgcgggccga tctgcccgcg
     2641 gcggaggaga aagtgcgcga cctggggctg gtgctggtgg gggcggaatc ggagcggcgc
     2701 atcgcccagt acatagaaaa atacaaaaac gtggtctgca tctccgagca ccacaccggc
     2761 cagccctgcg acctggtgct gctggacatg cgcaagtctc tgctgcgcgc ctgggagtac
     2821 ctgagcgagc tgggccaccg ccgcatcgct ttcgccggcg cggaaaaggg ggatttcttc
     2881 cgcacctata ccgacgtcat ccccgccgac gccggggccc agttcatccc ctgcgggctg
     2941 tcgatgagcg acggcatgct gctggccggg gagctctgcg actgtctgat ggtgccctcg
     3001 gcgctggtgt gtgagaataa ctccatcgcc tgcggggcca tccaggcgct gcgcgagcac
     3061 cgactggccg tgcccgggga cgtatcggtg gtcagcctgg gcggcgggct ggacctgtcc
     3121 cgcgcggcgc tgccggtgag cgtgacggtg ttctcttaca acaaggcgga gatcagccgc
     3181 ctggcggtgg acctgctgat cgaccgcatc caccggcggc acctgctcgc cgtgacctat
     3241 gagcccgggt gcgagatggc cgacaacggc agctgcgcgc ccgccaggga ggtgacctga
     3301 aatggcattc ggcatccgcg agatcgcccg tttggccggc gtgtcgccgg cgacggtctc
     3361 ccgcatcctg aacgaccccg cctaccgctg ttcctccgag gagcggcgcg cccgggtgct
     3421 ggagatcgcg cgggccaaca actatcagcc caacgaggac gccaagcggc tgcgcagcgg
     3481 cggcagcttt tcggccccgc aaaaaaagat ctgctgcttt ttggcccgca ccctgaccag
     3541 cgcccccaac ggggaccaat tcttctcctc cctggcccgc gcg
//
LOCUS       DBMDYA010000241         3575 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5972_length_3575_cov_2.158607, whole genome shotgun sequence.
ACCESSION   DBMDYA010000241 DBMDYA010000000
VERSION     DBMDYA010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5972_length_3575_cov_2.158607"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="ACZPFK_06890"
     CDS             <1..370
                     /locus_tag="ACZPFK_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406071.1"
                     /translation="AYMRTLSGGNMQKVITARECSSHPRLLIASQPTRGIDVGATEFI
                     RKRLVELRDQGAAILLFSADLAEIMQVSDRILVFHGGRIVAHIPRVSEIDENILGEYM
                     LGLRTQSEEEIGGAVFENKN"
     gene            354..1472
                     /locus_tag="ACZPFK_06895"
     CDS             354..1472
                     /locus_tag="ACZPFK_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406072.1"
                     /translation="MRTKTKNVEAIEKRFTVIRTLVGVGIALVIALALIAAVSDNPFE
                     ALMGFLTGPLTSVNRLGNVVEKAIPLLFTGTAVCIVFSCGQISLCIEGVFYITGIACA
                     AVATQSGIAAGLHPLLCILVAGVLGALICAIPALMYIRFDTMTVVSSLMINFICLYIG
                     KYLMHYVMRDPAAGFDASAKYEKSARLFKLFSKTNIHFGLVIAVIVVVVGFYALYKSP
                     WGYGVRMTGLSAGFAKFQGIGVAGMILTASMAGGFLAGMGGAVEQLGMYKRFSYDGLS
                     GHGWDGIMIAVIAQNNPKFVPLAALFVAYISTGADVVNRTCDVPIEIINIVQAVIIMF
                     VAAERFLAGWKQRKIVQASQQAQMEKLAGEQTEGAQKA"
     gene            1469..2296
                     /locus_tag="ACZPFK_06900"
     CDS             1469..2296
                     /locus_tag="ACZPFK_06900"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1406073.1"
                     /translation="MNKAYEKLQNCTRIVREKIGDFTPKIALVLGTGLGGFGEKVEHP
                     VVVDYHEIEGFPVSTVVGHAGRFVFGTVAGVPVVVMQGRVHYYEGYSMEDVVLPIRLM
                     GLLGAQVLFLTNGVGAVNRSYRPGDLMLMRDHLSLFIPSPLLGENMDELGPRFPDMTH
                     VYREDLCDAIRRAAAELRIRLQEGVYVQLTGPQYETPEEIRVLEKLGGDVVGMSTVCE
                     AIAANHMGLRVCGVSAIGNMGAGIRDEVLTHEPKDEVDQNFADLVTASIRNISQLLS"
     gene            2321..3304
                     /locus_tag="ACZPFK_06905"
     CDS             2321..3304
                     /locus_tag="ACZPFK_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406074.1"
                     /translation="MNDLMTFVLSPAFVNAIIRMSTPLIFVSMAAVLGAKANILCIAY
                     EGMMLFAALGGVIGSALSQNLLVGMLCGVLAGSLIAGLFAYFVLVLKTKDMLVGLALN
                     TLGSGGTIFALYIMSGQKASSISLNSLQFPVVNIPIIKDIPVLGDIVSGHNLLTYLAY
                     LSVILVYLLLYKTPLGLRIRSVGESPDAAESVGTDVVRTRVIALMIGGVLASFGGMFM
                     SMGYTPYFTRDMMNGRGFNAIAAQNLGAGHPLLTLLCSILFGAADAFGIALQTSRLPP
                     QLASMVPYITTLVGLIIIGQARRKQEKKRALALGARELAAEQEAAPRGVKG"
     gene            3301..>3575
                     /locus_tag="ACZPFK_06910"
     CDS             3301..>3575
                     /locus_tag="ACZPFK_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406075.1"
                     /translation="MRRLLIDTDTGGDDAVALIMALRDPQVRVEAVTTVMGNVEVAQA
                     TKNALLAIERAGTYAPPVYQGLENAILNEPLVNKNFTIHGRDGMGDLG"
BASE COUNT          628 a         1065 c         1194 g          688 t
ORIGIN      
        1 cgcctacatg cgcaccctct cagggggcaa catgcaaaag gtgatcaccg ccagggagtg
       61 ctcgtcccac ccgcggctgc tcatcgccag ccagcccacc cgcggcatcg acgtgggcgc
      121 caccgagttc atccgcaagc gtttggtgga gctgcgggac cagggggcgg cgattctgct
      181 gttttcggcc gacctggccg agatcatgca ggtcagcgac cggatattgg tgttccacgg
      241 cgggcggatc gtggcccata tcccccgggt gagcgagatc gacgagaaca tcctgggcga
      301 atatatgctg ggcctgcgca cccagagtga agaagagata gggggtgccg tgtttgagaa
      361 caaaaactaa aaacgtggag gccatcgaaa agcgcttcac cgtcatccgt accttggtgg
      421 gggtgggcat cgcgctggtg atcgcgctgg cgctgatcgc ggcggtgagc gacaacccgt
      481 ttgaggcgct gatgggcttt ctcaccgggc cgctcacctc ggtcaaccgg ctgggcaacg
      541 tggtggaaaa ggccatcccg ctgctgttca caggcacggc ggtctgcatc gtgttctcct
      601 gcggacagat cagcctgtgc atcgagggcg tgttttacat caccggcatc gcctgcgcgg
      661 cggttgccac ccagtcgggc atcgcggccg ggctgcaccc gctgctctgc atcctggtcg
      721 ccggggtctt gggggcgctg atctgcgcca tcccggcgct gatgtacatc cgctttgaca
      781 ccatgacggt ggtctcgtcg ctgatgatca actttatctg tctgtacatc ggcaaatacc
      841 tgatgcacta tgtcatgcgg gacccggcgg cgggcttcga cgcatcggca aaatatgaaa
      901 aaagcgcccg gctgttcaag ctcttttcca agacgaacat tcatttcggc cttgtcatcg
      961 ccgtgatcgt ggtggttgtg ggcttctatg cgctgtacaa aagcccctgg ggctacgggg
     1021 tgcgcatgac cggcctcagc gcgggttttg ccaaattcca gggcatcggc gtggcgggca
     1081 tgatcctcac cgcctcaatg gcgggcggtt ttctggccgg catgggcggc gccgtggagc
     1141 agctgggcat gtacaagcgc ttttcttacg acggcctgtc gggccacggg tgggacggca
     1201 tcatgatcgc ggtcatcgcc cagaacaacc ccaagttcgt gccgctggcg gccctgttcg
     1261 tggcctacat cagcaccggg gccgatgtgg tgaaccgcac ctgcgacgtg cccatcgaga
     1321 tcatcaacat cgtgcaggct gtcatcatca tgtttgtggc ggcagagcgc ttcctcgcgg
     1381 ggtggaaaca gcgcaagatc gtgcaggcgt cgcagcaggc gcagatggaa aaattggccg
     1441 gagagcagac ggaaggagcg cagaaagcat gaacaaggca tatgaaaagc tgcagaactg
     1501 cacccgcatc gtgcgggaga agatcgggga tttcaccccc aagatcgccc tggtgctcgg
     1561 caccgggctg gggggcttcg gagaaaaggt cgagcatccc gtggtggtgg attatcacga
     1621 gatcgagggc tttcccgtct cgacggtggt gggccatgcg gggcggttcg tcttcggcac
     1681 cgtcgcgggg gtgccggtgg tggtgatgca gggccgggta cattattacg agggctattc
     1741 catggaggat gtggtactgc ccatccggct gatgggcctg ttgggcgcgc aggtgctgtt
     1801 tctcaccaac ggggtgggcg cggtcaaccg cagctaccgc cccggggacc tgatgctgat
     1861 gcgggatcat ctctccctct tcatcccctc gccgctgctg ggcgaaaata tggacgagct
     1921 gggcccgcgg ttccccgata tgacccatgt ctaccgcgag gacctgtgtg acgcgatccg
     1981 ccgcgccgcg gcggagctgc gcatccgcct gcaggaaggc gtttatgtgc agctgaccgg
     2041 cccccagtat gagacgccgg aggagatccg ggtgctggaa aagctgggcg gcgacgtggt
     2101 gggcatgagc accgtgtgcg aggccatcgc ggctaaccac atgggcctgc gggtctgcgg
     2161 cgtctccgcc atcggcaata tgggcgccgg catccgggac gaggtgctca cccacgagcc
     2221 caaggacgag gtggaccaga acttcgccga cctggtgacg gcctcgatcc ggaatatttc
     2281 ccagctgctg tcttagcggc ggacgaccgg gaggataaca atgaatgatt tgatgacctt
     2341 tgttctgtcg cccgctttcg tcaacgccat catccgcatg agcacgccgc tgatctttgt
     2401 gtccatggcg gcggtgctgg gcgcaaaggc gaacatcctc tgtatcgcct acgagggcat
     2461 gatgctgttt gcggcgctgg gcggcgtcat cggcagcgcc ctgtcccaaa acctgctggt
     2521 gggtatgctc tgcggcgttt tggccggcag cctcattgcc gggctgttcg cctacttcgt
     2581 gttggtgctc aagaccaagg acatgctggt gggcctggcg ctgaacacgt tgggcagcgg
     2641 cggcacgatc tttgcgctgt acattatgtc ggggcaaaag gccagctcca tctcgctcaa
     2701 cagcctgcag ttccctgtgg tgaacatccc catcatcaag gatatcccgg tgctggggga
     2761 catcgtctct gggcacaacc tgctgaccta tctcgcctat ctgtcggtga tcctggtcta
     2821 cctgctgctc tacaagaccc cgctgggcct gcgcatccgc tcggtgggcg agagcccgga
     2881 cgcggccgaa tcggtgggca cggacgtggt taggacccgt gtgatcgccc tgatgatcgg
     2941 gggcgtgctg gcatcctttg gcggcatgtt catgtccatg ggctataccc cctatttcac
     3001 ccgggatatg atgaacggcc gcggcttcaa cgccatcgcc gcgcagaacc tgggcgcggg
     3061 acatcccctt ttgaccctgc tgtgctcaat tctgttcggc gcggcggacg cctttggcat
     3121 cgcgctgcag acctcccgcc tgccgccgca gttggcctcg atggtgccct acatcaccac
     3181 gctggtcggc ctgatcatca ttgggcaggc gcgccgcaag caggagaaaa agcgggccct
     3241 ggcactgggc gcacgggagc tggccgctga acaggaagcc gctcccaggg gggtgaaagg
     3301 atgagacggc tgctgatcga caccgacacc ggcggcgacg acgcggtggc gctgatcatg
     3361 gcgctgcggg acccgcaggt gcgggtggag gctgtcacca ccgtgatggg caatgtggag
     3421 gtggcccagg ccaccaaaaa tgcgctgctg gccatcgagc gggcgggcac ttatgcgccg
     3481 ccggtctacc aggggctgga aaacgcgatc ctcaacgagc cgctggtgaa taaaaacttc
     3541 accatccacg ggcgggacgg gatgggcgac ctggg
//
LOCUS       DBMDYA010000242         3566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5986_length_3566_cov_1.269264, whole genome shotgun sequence.
ACCESSION   DBMDYA010000242 DBMDYA010000000
VERSION     DBMDYA010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5986_length_3566_cov_1.269264"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="ACZPFK_06915"
     CDS             <1..874
                     /locus_tag="ACZPFK_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016959.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR1406076.1"
                     /translation="LSAQEEAARRLEQDVAAAKKEGETFKVKLFSSEKENYALRQEAA
                     ELRERAGRMEQALKEAEETGRALERARQEAAERAAQLEQLHTENERLSGRIGELELSL
                     DGWKAREAEIGRAEARAREDAARLQQEAGEEAARTEAEARRDADKILADANRAAVHLQ
                     EDAEAEALRRREAAVLGAREMDQNVQLLRGQLEAVERQMDEAYASLKAATERIQKAVA
                     DVGAQTEQVGGAAARLGGPEQAAESAQPSVAEPRSQEAPGPHRARPIPPHIAEPKARS
                     LSDLVLEKLAHILS"
     gene            1115..1426
                     /gene="trxA"
                     /locus_tag="ACZPFK_06920"
     CDS             1115..1426
                     /gene="trxA"
                     /locus_tag="ACZPFK_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1406077.1"
                     /translation="MAMEITAENFEEVVLKSTKPVVVDFWATWCGPCKMMGPVIDQLA
                     DELAATAVVGKVNVDEQQDLAARYGVMSIPTVVLFKDGKEAQRSVGFVPKAKLRTNLG
                     L"
     gene            complement(1488..2072)
                     /locus_tag="ACZPFK_06925"
     CDS             complement(1488..2072)
                     /locus_tag="ACZPFK_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1406078.1"
                     /translation="MGYKEEFISAFSEHIHRDGAEALLAWMQTTDFFTAPASTRFHGA
                     CESGLVMHSLNVYNVLCSRFFAEGENPESYAICGLLHDLCKANFYKPGFRNVKNDETN
                     TWERVPTYSVADAFPYGHGEKSVYLIERFLRLKPAEAMAIRWHMGGFDDSARGGSFAI
                     SEAYRAYPLAVKLHLADLEATYLVEKGTSAVNRK"
     gene            complement(2075..2296)
                     /locus_tag="ACZPFK_06930"
     CDS             complement(2075..2296)
                     /locus_tag="ACZPFK_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIR1406079.1"
                     /translation="MAEFKYEIVERIAVLSQSPNGWERQLNLVSWNEREPKYDIRDWA
                     PDGSKMGKGISLSHDELAILKGILAEMEI"
     gene            2640..>3566
                     /locus_tag="ACZPFK_06935"
     CDS             2640..>3566
                     /locus_tag="ACZPFK_06935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR1406080.1"
                     /translation="MSGETIYTVTLNPAWDRTAEVERLQPGGTHLLGAVRGDAGGKGI
                     NVSRVLCALGQPTCALGLLGGGAGDELDQALRQEGVPAAFVRLAAACRVNTKIVDRAA
                     GVTTEFNEMGPRVDDGALEQLEARLKGLPQGAVTVLAGSLPPGAPARWYARAAELCHD
                     RGGRVVLDASREALRLGLEGEPEIVKPNRDELACLCGGASLREEQLPAAARALLRGRT
                     RLAAVSLGADGALFVTDQFVVRVKAPRVRAVSTVGAGDTMTPMWISIITTVVLRVPIA
                     YGIAFFTRSAEYPTGRPESTFISLLVSWSLGAL"
BASE COUNT          667 a         1054 c         1260 g          585 t
ORIGIN      
        1 gctgagcgcg caggaggagg cggcccgcag gctggagcag gacgtggcgg ctgcgaaaaa
       61 agagggggaa acgttcaaag taaagctgtt ctcctccgaa aaggaaaatt atgccctgcg
      121 ccaggaggcc gccgagctgc gcgagcgggc gggccgcatg gaacaggcgc tgaaagaggc
      181 cgaggagacc gggcgcgccc tggagcgggc acggcaggag gccgccgagc gcgctgccca
      241 gttggagcag ttacatacgg aaaatgagcg cctcagcggg cgcattgggg agctggagct
      301 ctccctggac gggtggaaag cccgggaggc ggagatcggg cgggcagagg cccgcgcgcg
      361 ggaggatgcg gcccggctgc agcaggaggc gggcgaagaa gcggcgcgga cagaggccga
      421 ggcccgcagg gacgcggaca agatcctcgc cgacgccaac cgcgccgccg tccatctgca
      481 ggaggatgca gaggcggagg cactgaggcg gcgcgaggcc gcggtgctgg gcgcccggga
      541 gatggatcag aacgtccagc tcctgcgcgg ccagctggag gcggtggagc gccagatgga
      601 cgaggcgtat gcctccctca aggcggccac cgagcgcatt caaaaggcgg tggcggacgt
      661 gggggcgcag acagagcagg tgggcggcgc tgcggcccgc ctcggcggcc cggaacaggc
      721 ggcggaatcc gcacagccgt cggtggctga accccggtct caagaagcac ccggcccgca
      781 cagagcccgg cccattccgc cccacatagc cgagcccaag gcgcggagct tgtccgacct
      841 ggtgctggag aagctggccc atatcctcag ttgaaccgca ggagccgtga aagccgtttt
      901 cacggctcct gttgtttttc gccgcatacc cgggtgcggc tggtgatata ataaaagaaa
      961 gaaaagcttt ttgccgcaaa atatgtttag ctgggaagcg gggccccgac aggcggctgc
     1021 tgccgcaggt gaaaacaaga cttgcaataa ctatactgta atggtataat attcctgcaa
     1081 gaaaatttat tattttttga ggaggctgat ccgtatggca atggagatta ccgcggaaaa
     1141 tttcgaagag gtcgttttaa agagcacgaa gcctgtggtc gtggatttct gggccacctg
     1201 gtgcggcccc tgcaaaatga tgggcccggt catcgaccag ctggcggacg agctggcggc
     1261 cactgcggtg gtgggcaagg tcaacgtgga cgagcagcag gatctggcgg cccgctacgg
     1321 cgtcatgagc atccccacgg tggtgctgtt caaagacggc aaagaggcgc agcgcagtgt
     1381 gggctttgtg cccaaggcaa aactgcgcac caacctgggc ctgtaacgcc ggagaagcaa
     1441 caataaaaaa gggcggaaat ccaaggattt ccgccctttt ttattgctta tttccggttg
     1501 accgcgctgg tgcccttttc gaccagatag gtggcctcca gatcggccag atgcagcttg
     1561 accgccagcg ggtaggcgcg ataggcctcg ctgatggcaa agctgccgcc ccgcgccgag
     1621 tcgtcaaagc cgcccatgtg ccagcggatc gccatcgcct ccgccggctt gaggcgcaga
     1681 aagcgctcga tcagatacac gcttttttcg ccgtgcccgt aggggaacgc atcggccacc
     1741 gagtaggtgg gcacccgctc ccaggtgttg gtctcgtcgt ttttgacgtt gcggaacccg
     1801 ggcttataaa agttggcctt gcacaggtcg tgcagcagcc cgcagatggc atagctctcc
     1861 gggttttccc cctcggcaaa aaagcggctg cagagcacgt tatagacgtt gaggctgtgc
     1921 atgaccaggc cgctctcgca ggcgccatga aagcgtgtgc tcgcgggcgc ggtgaaaaag
     1981 tcggtggtct gcatccacgc cagcagggcc tcggccccgt cgcggtggat gtgttcgctg
     2041 aaagcgctga tgaactcttc tttatacccc atgctcagat ttccatctcg gcgaggatgc
     2101 ccttgaggat cgccagctcg tcgtgggaca gggagatgcc cttgcccatt ttgctgccgt
     2161 ccggcgccca gtcgcggata tcgtatttcg gctcccgctc gttccagctc accaggttca
     2221 gctggcgctc ccagccgttg gggctctggg agagcaccgc gatcctctcc acgatctcat
     2281 atttaaattc tgccatcgta tccaccatcc taagtccttg atttgcagcg gctggcctct
     2341 gcaacaggcg gccgccgtac tgattttcta ttgtaaaggt ttggcccctc aaatgcaagc
     2401 cctaaataaa gagggagaaa aggggaagcg atacttgcac ggggccggca ggacgggtag
     2461 aatggaaaaa gagagggcgc aaagcggggc tttctccttt gcttttttgc cgtccggcgc
     2521 cggtgggcgt ccggggcgga aaaggggata ggagagcggc tttcccgccg ggaggcggga
     2581 gccgggccga cggcggcgca gcgggaaaaa agagaaaaga caaggatagg ggtggagaga
     2641 tgagcggaga aacgatctat accgtcacgc tgaacccggc gtgggaccgc acggccgagg
     2701 tggagcggct gcagccgggc ggcacccatc tgctgggcgc cgtgcgcggc gatgcgggcg
     2761 gcaaggggat caacgtcagc cgcgtgctgt gcgcattggg gcagcccacc tgcgcgctgg
     2821 gcctgctggg cggcggcgcg ggggacgagc tggaccaggc cctgcggcag gagggggtac
     2881 cggccgcgtt tgtgcgcttg gccgccgcct gccgcgtcaa caccaagatc gtcgaccggg
     2941 cggcgggcgt cacgaccgag ttcaacgaga tgggcccccg ggtggacgac ggagcgctgg
     3001 agcagttgga ggcgcggctc aaagggctgc cacagggcgc ggtgaccgtg ctggcgggca
     3061 gcctgccgcc gggagcgccg gcccgctggt acgcccgggc cgccgagctc tgccacgacc
     3121 gaggcggccg ggtggtgctg gacgcctcgc gggaggcgct gcgcctgggc cttgaggggg
     3181 agccggagat cgtcaagccg aaccgcgatg agctggcctg cctctgcggc ggcgcgtccc
     3241 tgcgggaaga acagctgccc gccgcggcgc gggcgctgct gcggggccgc acccgcctgg
     3301 ccgcggtcag cttgggggct gatggggcgc ttttcgtcac cgaccagttt gtggtgcggg
     3361 tgaaagcgcc ccgggtgcgc gccgtcagca ccgtgggcgc gggcgacacc atgaccccca
     3421 tgtggatctc catcatcacc actgttgtgc tccgcgttcc cattgcctac ggcatcgcat
     3481 tctttacacg cagcgccgag tatcccaccg ggcgcccgga atccaccttt atctccctgc
     3541 tggtctcctg gtcgctgggc gcactg
//
LOCUS       DBMDYA010000243         3561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_5995_length_3561_cov_1.320520, whole genome shotgun sequence.
ACCESSION   DBMDYA010000243 DBMDYA010000000
VERSION     DBMDYA010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_3561_cov_1.320520"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            332..1312
                     /locus_tag="ACZPFK_06940"
     CDS             332..1312
                     /locus_tag="ACZPFK_06940"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1406081.1"
                     /translation="MEVSWLLVQQIASMVLMGLAGYGVGKLKLITGEQSRVLSCMCIY
                     LAVPCSLMTSFNTAMEADKLTGLLLALPLLDGDSLLRIEPPFESRPYVELTLDVLARF
                     GIAARWRNSLSLSVPGGQRYRPVPRFAVEGDYSQAAFFAALGALAGRVECTGLRPGSA
                     QGDRAIFEFLARMGAPAARAASALVCRRAPLRPGVFDLADCPDLGPILTVLCCFCDGV
                     SRIEHVGRLRYKESDRIAAMQAELSKLGLTVRCEGDALVIPGIGERACRAPAEPLSGW
                     NDHRVVMALAVAASRADGPVAIEGAEAVEKSYPHFFEDLQSAGVRVELSV"
     gene            1410..2261
                     /locus_tag="ACZPFK_06945"
     CDS             1410..2261
                     /locus_tag="ACZPFK_06945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR1406082.1"
                     /translation="MIAEDTRFLIVGLGLIGGSYARGLSKKGFWVDAIDTDPDSIAYA
                     LENGLIRRGSTWADPALARGADIVVFALYPGVMIEWVRQNQGLFRPGALLTDVSGVKC
                     HVVDAIQGFLRQDLEFIGSHPMAGKEVYGVRNSDEGIFKIANFIVTPTEKNTSGAVET
                     AREIGRLLEFHTITELAPAAHDEIVGFVSQLTHAIAVSLMTCNDDEDLQNYTGDSFRD
                     LTRIARINEKMWSELFLLNRQELISQIDSFTAELLRMRGMLERQDRAALEEMFIRSTQ
                     RRALFDR"
     gene            2303..3184
                     /locus_tag="ACZPFK_06950"
     CDS             2303..3184
                     /locus_tag="ACZPFK_06950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1406083.1"
                     /translation="MPEARLVKNVRENDALRSSFDALAQRVFGISFEDWYLRGYWGEA
                     YRPYVLAEGETVLAGAAANLMDLRWDGEVHRCIQIGTVMTAPEARGRGLSRRLLEELL
                     CDWAEDAELVYLFANATALGFYPKFGFQRQAEQRCFVPAPASRPEAVRRLNMDDPADR
                     QLLWACALRGNPFSALSLWGGYELLMFHCTGPHRENVFYLPQAGAAVVAAQENGRLLC
                     LDIFGGEGKRLEEVLAPLVRATDREVGLGFTPLPDAVPRRRFEPVGEDDALFLLKGGL
                     SPFGGGEPLLFPLLSHA"
BASE COUNT          596 a         1135 c         1187 g          643 t
ORIGIN      
        1 tcctcctgga tcaagtgata agaccggccg gcacgccccg gcggcaagaa gaccttagca
       61 aaaagaggcg gacagggcaa ccaaaagagg catacaattt gacaactaac agttgcatcc
      121 gggatttttt caactttttg gtccaaaaac gggaaaagag acaggcgtcc gccggggctt
      181 atcttaccac agcacaccgg gattgacaag cgcggtgggc ggacagagca cggggaaagg
      241 attgacaaac acgatgcgga ccgctagact ggggaagatg aagtcccggg tgctggcggc
      301 cccgtggcgt gagattggga ggagagcgcg gatggaagtg agctggctgc tggtgcaaca
      361 gatcgccagc atggtgctga tgggtctggc cggctatggt gtgggaaaac tgaagctgat
      421 cacaggggag cagagccggg tgctctcctg catgtgtatc tatctggcgg tgccgtgcag
      481 cctgatgacc tccttcaaca cggccatgga ggcggacaag ctgaccggcc tgctgctggc
      541 cctgccgctg ctggacgggg actcgctgct gcggatcgag ccgcccttcg agtcccgccc
      601 ctatgtggag ctgaccctgg atgtgctggc ccggttcggc atcgccgccc gctggcggaa
      661 cagcctttcc ctctctgtgc ccggggggca gcgctaccgc cccgtccccc gctttgcggt
      721 ggagggagac tactcccagg ccgccttttt tgcggcgctg ggggcgctgg cggggcgggt
      781 ggaatgcacc ggcctgcggc cgggctcggc ccagggcgac cgggcgatct ttgaatttct
      841 cgcccgcatg ggcgcgccgg cggcaagggc ggcgtccgcc ctcgtctgtc gccgcgcgcc
      901 gctgcgcccg ggcgtttttg atctggccga ctgccccgat ctcggcccca tcctcaccgt
      961 gctctgttgc ttctgcgacg gcgtctcccg catcgagcat gtggggcggc tgcgctacaa
     1021 agaatccgac cgcatcgccg ccatgcaggc cgagctgtca aagctggggc tcaccgtgcg
     1081 ctgcgagggg gacgcgctgg tcatcccggg catcggtgaa agggcctgcc gcgccccggc
     1141 agagcccctc agcggctgga acgaccaccg cgtggtgatg gcgctggccg tggcggcctc
     1201 gcgcgccgac ggcccggtgg cgattgaggg cgccgaggcg gtggaaaaat cttaccctca
     1261 cttttttgag gacctgcaaa gcgccggcgt gcgggtggag ctgtccgtct gatcggcggg
     1321 cggcgcggcc cccaaggcgc gccgcccttt tttcagcgcc cgcgtcgcgg ccaggcgggc
     1381 ccaaaaacaa cagaaacgag gattgcgaca tgattgcgga ggacacccga tttctcatcg
     1441 tcggcctggg gctgatcggc ggcagctacg cccgcggact ctccaaaaaa ggcttttggg
     1501 tcgacgccat cgacaccgac cccgacagca tcgcctacgc gctggaaaac ggcctcatcc
     1561 gccggggctc cacctgggcc gaccccgcgc tggcgcgcgg cgcggacatc gtggtgttcg
     1621 ccctctaccc cggcgtgatg atcgagtggg tgcgacagaa ccagggcctt tttcggccgg
     1681 gcgcgctgct caccgacgtg tccggcgtaa aatgccatgt ggtggacgcc atccagggct
     1741 ttttgcgaca ggatctggag ttcatcggca gccaccccat ggcgggcaag gaggtctacg
     1801 gggtgcgcaa cagcgacgag ggcattttta aaatcgccaa cttcatcgtc acccccactg
     1861 aaaagaatac ttccggggcg gtggagaccg cgcgggagat cggccgcctg ctggagtttc
     1921 acaccatcac cgagctggcc cccgccgcac acgatgagat cgtggggttc gtctcccagc
     1981 tcacccacgc cattgcggtg agtctgatga cctgcaacga cgacgaagac ctgcagaatt
     2041 ataccgggga ctcctttcgc gatttgaccc gcattgcccg catcaacgaa aagatgtgga
     2101 gcgagctgtt cctgctcaac cggcaggagc tgatcagcca gatcgacagc tttaccgccg
     2161 agctgctgcg gatgcgcggc atgctggagc ggcaggaccg ggcggcgctg gaggagatgt
     2221 tcatccgctc cacgcagcgc cgggcgctgt tcgaccgctg aaacgccgcg tcgcaagggg
     2281 cgcagaaaag ggagggcagc ccatgccgga agcaagactg gtcaaaaacg tccgggagaa
     2341 cgacgcgctg cgcagcagct tcgacgcgct ggcacagcgc gtcttcggca tctcgtttga
     2401 ggactggtac ctgcgcggat actgggggga ggcctatcgg ccctatgtcc tcgccgaggg
     2461 ggagacggtg ctggcaggcg ccgccgcaaa tctgatggac ctgcgctggg acggcgaggt
     2521 ccaccgctgc atccagatcg gaaccgtgat gaccgccccc gaagcgcggg ggcgcggcct
     2581 ctcccgccgc cttttggaag agctgctgtg cgactgggcg gaggacgccg aactcgtgta
     2641 tctgtttgcc aacgccaccg cgctgggctt ttatcccaaa ttcggctttc agcgccaggc
     2701 cgagcagcgc tgctttgtgc ccgccccggc gtcgcggcct gaggcggttc ggcgcttgaa
     2761 tatggacgac cccgccgacc ggcagctgct gtgggcctgc gcgctgcggg gcaatccgtt
     2821 ttccgccctt tcgctgtggg gcggctacga gctgttgatg ttccactgca ccggccctca
     2881 tcgggaaaac gtcttttacc tgccgcaggc gggggcggcc gtcgtcgccg cgcaggaaaa
     2941 cggtcgcctg ctctgcctgg acatcttcgg cggagagggc aagcgtctgg aggaggtgct
     3001 cgccccgctc gttcgggcaa ccgaccggga ggtcgggttg gggttcactc ctctccccga
     3061 cgccgtcccc cggcgccgct ttgagccggt gggagaggac gacgccctgt ttctgctgaa
     3121 aggcggcctc tcgcctttcg gcggcgggga gccgctgctt tttccgctgc tgtcccacgc
     3181 ctgagcgcgg atgtccgccc tgttttagcg cattgaaaag tggcatgtga aaccatctgt
     3241 cacagacaaa aacggagaca ggctgtgccc gtctccgttt ttttgtttta agcggtatcc
     3301 ccgcacgcgc ttgtcgccgg agcttcatcc tgttatcagc gaaatttgtc tgtgacaaat
     3361 ttccgttccg cgttgtttca agacatcccg tcgccctttt gcacaatttt cttttgagcc
     3421 cgcacaaagc ggcaaaaccg ccgcctgggg caaggcggcc ccagcgtcgc cttgtcccgg
     3481 acggcggttt tgcggggcag ccccagcccg cggccctact ccacctcgat ggcgttgacc
     3541 gggcagccgg cctgtgcgtc c
//
LOCUS       DBMDYA010000244         3556 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6007_length_3556_cov_1.723878, whole genome shotgun sequence.
ACCESSION   DBMDYA010000244 DBMDYA010000000
VERSION     DBMDYA010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_3556_cov_1.723878"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="ACZPFK_06955"
     CDS             <1..322
                     /locus_tag="ACZPFK_06955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1406084.1"
                     /translation="GVVHCYSGSAEDVKWLAAQGLYLGFGGVVTFKNARKTLTAAAAV
                     PADKLLIETDCPYMAPTPYRGKRNDSSLLCYVAETLAALRGVEPEALLAQTMANGRAL
                     FGIR"
     gene            1504..2478
                     /locus_tag="ACZPFK_06960"
     CDS             1504..2478
                     /locus_tag="ACZPFK_06960"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1406085.1"
                     /translation="MKRNPVKEALLRVLRAAPGQWQSGEALAEAAGVTRSAVWKAAGE
                     LRAEGFPLESAPRRGYRLEAGGDLLTREELLAHLGAGWQVECRPALPSTNRRARELAL
                     AGAPAPLLVAAAEQTEGKGRRGRSFFSPAGSGLYFSLLLRPALAASDAPLVTAAAAVA
                     ARRALAGFGVEVGVKWVNDLWLQGRKVCGILTEAATGFESGLVEHLIVGFGLNLTEPE
                     GGFPPALAAIAGPVFASGTAPGRAALAAAVTREFLPLLGALPEKGFLPEYRAASILPG
                     RCVTVLPALGEPYEATAGEIDDDGRLAVFLPDGERRLLHAGEVRLRPH"
     gene            2842..3369
                     /locus_tag="ACZPFK_06965"
     CDS             2842..3369
                     /locus_tag="ACZPFK_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1406086.1"
                     /translation="MEHTVKKTRELVLASLMTAATAVLAQIMLPIGPVPFNLAVLGAY
                     LAGCLLEPAWALASMAAYLLLGAFGLPVFAGLSGGPAVLAGPTGGYIVGYLLIALFTA
                     LAAKRGGTLLISAAMAAGLLACYALGTGWFMVVTGRGLAESLALCVLPFVIPDACKAA
                     AALLLSRAVRARLRG"
BASE COUNT          499 a         1185 c         1158 g          714 t
ORIGIN      
        1 cggtgtggta cactgctatt ccggcagtgc ggaggatgta aaatggctcg ccgcccaggg
       61 gctgtacctc ggcttcggcg gggtggtgac ctttaaaaac gcccgcaaaa cgctgactgc
      121 cgcggccgcc gtgcccgcgg acaagctgct gatcgagacc gactgcccct atatggcccc
      181 cacgccctat cggggcaagc gcaacgattc ctctctgctc tgttatgtgg ccgagacgct
      241 ggccgccctg cggggcgtgg agccggaggc gctgctggcc caaaccatgg caaacggccg
      301 cgcgctgttc ggcatccgct gacgtccttt tctgcgccgc agccgccatg cccggtctct
      361 ctttcgccgt ggaaagctgt ggtgaaagaa ataacaggat ggcgccatcg cgctctctgc
      421 gcaccgccgt tttccctttt cgaaaaaagc tccggagcag ccgataaagc gaccgacgga
      481 gggcggcacc aaccaaaagc gccgccctgc tgctccgccg tctcgccgtt ctaccctgac
      541 gccgccgctt ggcggcgttt tcagttggtg gcgtcctctt tctccgtttt ggctgaggcc
      601 caaccgggag atgtttttcc gttttttcgc cgtcgccggt atcagcgcgc gccgcagctt
      661 ggcgcgcttt caggtggtgc gttcctcctc gctgtagctg aaacccgggg gagttgtttt
      721 ttgctgtttt tcgctgtcgt ctgcgtcggc acacgccgct gctgggcgcg ctttcaattg
      781 gttcgtctcc cctgttttag atgaaatcca accggggctt ttgctgtttt tcgctgccgc
      841 cggcgccggc gcgcgtcacc gcttggctgc gctttcggtc tgtgtacctc tccctccact
      901 ttggctgaga cccagctggg gggtgttttg ccctctttcg ctgtcgccgg tatcggcgcg
      961 catcgcagct tgccgcactt ttggttggtg cttttctctt ccccacttcg gctgaggccc
     1021 aaccggagga tatttttccg tcttccgctg cccttggcgc cggcgcgcgt cgccgcttgg
     1081 cggcgttttc catcggcggt gcccctcttc tcttcggttc aaaccctgca aagcgtggcg
     1141 tcaaaagggt ccagggcgcc gggcgggcag gggtgcgcac ggcaaagaga agagcaaagc
     1201 taagtcgccc ccgcgacctc ttgtgcccgg cggcacgccc cgtccgctta ccgctcccgg
     1261 cctcctttca actcccgctc agctgctcgt cgccccaagg ccgcgcccct ctcaggcatt
     1321 caacggcgtc ttaaggcatt tttgtacttg cacttccatt ttggctttat tgtcaactaa
     1381 aaatattttt tagttgacaa taaagccgtt ttcttttaca ctgaaaggcg accaaggtca
     1441 attacatctg cgcctctttg tctgccgcct ggccgataaa agaggggccg gcaaggaggc
     1501 cccatgaaaa ggaaccccgt gaaagaagcg ctgctgcggg tgctgcgcgc cgcgccgggc
     1561 caatggcaga gcggcgaggc gctggccgag gcggccgggg tgacccgcag cgccgtgtgg
     1621 aaagccgcag gcgagctgcg ggcggagggc tttccgctgg aatccgcgcc ccggcgcggc
     1681 taccggctgg aggccggcgg cgacctgctc acccgcgaag agctgttggc ccatctgggg
     1741 gccggttggc aggtggagtg ccgccccgca ctgccctcta ccaaccgccg cgcgcgggag
     1801 ctggccctcg ctggcgcgcc tgcgccgctg ctggtggctg ccgcggagca gactgagggc
     1861 aagggccgcc ggggacgcag cttcttttcc cctgccggca gcggccttta tttcagcctg
     1921 ctcctgcgcc ccgcactggc ggcgtcggac gcgccgctgg tgaccgcggc ggcagcggtg
     1981 gccgcgcggc gggcgctggc cggctttggc gtcgaggtgg gggtcaaatg ggtcaacgac
     2041 ctctggctgc agggccgcaa ggtgtgcggc attctcaccg aggcggcgac cgggttcgag
     2101 agcggccttg tggagcactt gatcgtcggc ttcgggctca acctcacgga gccggagggc
     2161 ggctttcctc ccgcattggc ggccatcgcc gggccggtct ttgcgtcggg tacggcgccg
     2221 gggcgcgccg ccctggccgc cgccgtcacc cgggagtttc tgccgctgct gggggccttg
     2281 cccgaaaagg ggtttcttcc agagtaccgg gccgccagca tcctgcccgg ccgctgcgtc
     2341 accgtactgc cggccctggg ggaaccctat gaggcgaccg ccggggagat cgacgacgac
     2401 ggccgcctgg cggtgttttt gccggacggg gagcgccgtt tgctccacgc cggagaggtg
     2461 cggctgcgcc cccattgaaa aagcgccgcc ctcacgcctc accctgcgga aggaataaaa
     2521 cggcttttcc gccgggtggg gtgccgccgc ccgcgcccca cagcgccggg gcgcggcggt
     2581 ttttaccgca aagcgataaa gctccgcccg gcaacacctc tccccatcgg gctgctcccc
     2641 gcagcgcttc ccaggcgctc ttcggtaagc gcccggcttc caggggtcgg cggggccgca
     2701 caatggcccc ttgcgccctg tcggctgctc cacgggccgg gacgcttttc tgtccatcgg
     2761 agaggagctt gtctgtcttc gtccccggag gacagagaaa aaatttttcc gttttacctt
     2821 gcggaagtat tggaggaatt tatggaacac actgtcaaaa aaaccaggga actggtgctc
     2881 gcctcgctga tgaccgccgc caccgccgtg ctggcgcaga tcatgctgcc catcgggccg
     2941 gtgcccttta acctggccgt gctgggcgcc tatctcgccg gctgcctgct ggagcccgcc
     3001 tgggcgctgg ccagcatggc cgcctacctg ctgttggggg cgtttgggct gcccgtcttt
     3061 gccggcctct ccggcggccc agccgttctg gcaggcccca ccggcggcta catcgtcggc
     3121 tatctgctga tcgccctgtt caccgcgctg gcggcaaagc gcggcggcac gcttcttatt
     3181 tcggcggcca tggcggcggg cctgctggcc tgctatgccc tgggcaccgg ttggttcatg
     3241 gtcgtcaccg ggcgcggcct ggcggagagc ctggcgctct gcgtgctgcc tttcgtcatc
     3301 cctgacgcct gtaaggccgc ggcggcgctg ctgctctccc gggcagtgcg cgcccgcctg
     3361 cggggctgag acgtcaaaaa atccccggga gctgagaaga ggttttcagc ggcgccgacg
     3421 ggcgcttggc ccgcaaaaag ccaaagacgg gccggtgggt tgaactgcac ccaaaaagtt
     3481 agacaaagat atgatcaagc agccaaaagg gcttgctgtc tgtgaactgc aggcggtaag
     3541 ccctttagtt ttgcct
//
LOCUS       DBMDYA010000245         3546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6023_length_3546_cov_1.582874, whole genome shotgun sequence.
ACCESSION   DBMDYA010000245 DBMDYA010000000
VERSION     DBMDYA010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_3546_cov_1.582874"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="ACZPFK_06970"
     CDS             complement(<1..468)
                     /locus_tag="ACZPFK_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406087.1"
                     /translation="MKLSAREVAVFGMLGAAMYASKLLMEFLPNVHLLGVFVVAITVV
                     YRKKALYPIYLYVLVNGLFAGFALWWVPYLYLWTVLWGAVMLLPRRMPPRVRPLVYMA
                     VCAGHGFLFGTLYAPAQALLFGLDFKGMLAWIVAGLPWDFVHGVSNFFCGVLIV"
     gene            complement(465..971)
                     /locus_tag="ACZPFK_06975"
     CDS             complement(465..971)
                     /locus_tag="ACZPFK_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIR1406088.1"
                     /translation="MTKIKRSLSLFLCTVLIAATALAVTGCSGKDDAAGGSGSSAAAG
                     SSTAQVSSSAAQPGSSTAQTGGKVLGEGKTVFTFVVTDGDGNETAFEIHTDKATVGEA
                     LTELGLIAGEEGQYGLYVTEVNGIAADYDKDGVYWAFYVDGEYASTGVDATEVTAGAA
                     YALKLEKA"
     regulatory      complement(1029..1236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2170..2811)
                     /locus_tag="ACZPFK_06980"
     CDS             complement(2170..2811)
                     /locus_tag="ACZPFK_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1406089.1"
                     /translation="MKEILCFGDSNTHGYMPGVGTRYPMDVRWSGRLAGLLGEGYHVV
                     EEGLDGRTTAFEDELQPWRSGLGYIGGCVKSHAPLDLIIIMLGTNDAKTRYGVSADEI
                     GFGLRELIAKIETFFRYNGAHYGPCPKLLVVSPVPMPATGGDPEFNEESLKKQAGLKA
                     VYRPIAEEMGCAFAAAEDWIGPDLLGEDYCHYLPEAHRIFAERMAGLVKELLG"
     gene            complement(2925..3236)
                     /locus_tag="ACZPFK_06985"
     CDS             complement(2925..3236)
                     /locus_tag="ACZPFK_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406090.1"
                     /translation="MYELQIFFTKIPPSQNFPAPAGRAAGGQGTVMLPQNCPLKNDTR
                     NSNPYYTAEKMQSQCSRGHEKNYFCAESTGQGLMKNEMLSFVHNKTRIWQKNKEKRKR
                     R"
     gene            3211..>3546
                     /locus_tag="ACZPFK_06990"
     CDS             3211..>3546
                     /locus_tag="ACZPFK_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIR1406091.1"
                     /translation="MKKIWSSYKSSFILLGCMVLGGIVGAFWGPGASVLTPWANIFLN
                     LLYCVVVPMIFVSLVSAIAGMQNMRKLGKLLAVLLVLFLITGIVCSAYMVGFSALFDP
                     AKNTDMTQFT"
BASE COUNT          643 a         1161 c         1021 g          721 t
ORIGIN      
        1 cacgatcagg acgccgcaaa aaaagttgct gaccccgtgc acaaaatccc agggcaggcc
       61 ggcgacgatc caggcgagca tccccttgaa atccagcccg aacagcagcg cctgggccgg
      121 ggcgtacagg gtgccgaaca gaaagccgtg cccggcgcag acggccatat acaccagcgg
      181 ccgcaccctg ggcggcatcc gccgcggcaa aagcatcacc gccccccaca gcacggtcca
      241 cagatagaga tagggcaccc accagagggc aaagcccgca aacaggccgt tgaccagcac
      301 gtagaggtag atgggataca gcgccttttt gcggtaaacg actgtgatcg ccaccacaaa
      361 cacgccgagc agatgcacgt tgggcaaaaa ctccatcaac aatttggagg cgtacatggc
      421 ggcccccagc atcccgaaga ccgctacctc cctggcgctc agcttcacgc tttttcgagc
      481 ttgagcgcat aggccgcgcc cgccgtgacc tcggtggcgt ccacgccggt ggaggcgtac
      541 tccccgtcca catagaacgc ccagtagacg ccgtccttgt catagtcggc ggcaatcccg
      601 ttcacctcgg tgacatagag gccgtactgg ccctcctcgc cggcaatcag gcccagctcc
      661 gtcagcgcct cgcccacggt ggctttgtcg gtgtggatct caaacgcggt ctcattgccg
      721 tccccgtcgg tcaccacaaa ggtgaagacg gtcttgccct cgcccagcac cttgccgccc
      781 gtctgcgctg tggaggagcc gggctgcgcc gcgctggagc tcacctgcgc cgtggaggaa
      841 ccggccgccg cgctggagcc ggaaccgccg gccgcgtcgt ctttgccgct gcagcccgtg
      901 accgccagtg ccgtcgccgc gatcagcacg gtgcacagga agagcgacag tgatcttttg
      961 atctttgtca tctgcatcat cataatctcc tggattttta tttcctccca gccggcgctc
     1021 gcggctctat acggaggttt tgggccgaaa tattccggct tggcgccccc ggcggcccgc
     1081 cttctcggat tgctccaatg gcttgtcctc caaacgcggc tttggagggg accgtcggtc
     1141 ttgctgcgcg ccttacggcg acgggctcgc gcaggtcttg cacctgcttc cttcgacggc
     1201 gcttattata acacatcggc ccaccggatt caaaccgtcc cggggggccg ggggaaagcc
     1261 ttgcgggaaa accgaccgcc cccggtttta tgcctccctc ctatgggggc gcagagggcg
     1321 aaagtcggag gcttctcctc cgcaagagtc ccacgtcccc tgcctcacgt ggggctggcg
     1381 cgggactggc ccagggcggc tttcctccac aggtcccggc cgcatgcctg ctctcttata
     1441 aagcgcaggg gggccgaccg ctccccttgc ccgcttttcc ccgccgaaaa cgcgctgcgc
     1501 ccaggtggcc gcagcagcgc aacgcggcgc ggcccggcgg cgtttccaat ctggcggcag
     1561 tgttttcggc ctgacgacgg ggcgactggc cggagaaaat cgcaagaggc ggcggggccg
     1621 tgcttggcgc cttttcaaaa acggctttcc acagcgccgt cgcaacaaaa aaggcgcgga
     1681 gagcagcccc catgtctttg aagcggcccc ccaaagctgg ataaaagcag ggattgagca
     1741 gccgcaagag cccacagtct gtgagaagcg ggcgacacac ccctaagctc tgctttaatg
     1801 cgttaggagt gaaacgccgt gagctctgtc tcccacttcc ggtttggcct ctttgcttca
     1861 aagctccgtt ccaacaaatg gggggcgatt ttgcccacct cgcctcttgg tatcctgttg
     1921 cgtccccttg gtttcactat aatcatccta cctaataata tagagtgcgg ttcgcttttc
     1981 tgaaagcaat tctcgcagcg ctgtcaaaaa taaaaaagga accggagagc tccagcgcct
     2041 tttccagggc attccccgcc ctgccgcaca aaaaacaggg gcgcggtgcc cggctcttgc
     2101 ggccgtcccc ccgcgcccct gtttcgcgct tttccgatcc gcttccctgc ggcccgccgt
     2161 ccccccggct cagcccagaa gctcttttac caggcccgcc atccgctcgg caaagatgcg
     2221 gtgcgcctcg ggcagataat ggcagtagtc ctcgcccagc aggtcggggc cgatccagtc
     2281 ctccgccgcc gcgaacgcac agcccatctc ctcggcgatg gggcggtaca ccgctttcag
     2341 gcccgcctgc tttttcagcg actcctcatt gaactccggg tcgccgccgg tcgcgggcat
     2401 cggcaccggg ctgacgacga gcagcttggg gcagggaccg taatgggccc cgttgtagcg
     2461 gaaaaaggtc tcgattttgg cgatgagctc gcgcagcccg aagccgatct cgtccgccga
     2521 gacgccgtag cgggtcttgg cgtcgttggt gcccagcatg atgatgatca ggtccagcgg
     2581 ggcatggctt ttgacgcagc cgccgatata acccaggcca ctgcgccagg gctgcagttc
     2641 atcctcaaag gcggtggtgc ggccgtccag cccctcctcc accacgtgat agccctcgcc
     2701 cagcaggccc gcgaggcggc cgctccagcg cacgtccatc gggtaacggg tgcccacgcc
     2761 cggcatatag ccgtgggtgt tggaatcgcc aaaacacagg atctctttca caatgatcgc
     2821 tccttttgct gcgccccgcc ccgggcaaga cgcggattct gcctttaagc atagcgctcc
     2881 ccgccgcaaa aatcaaggga attgtctgca tatcaaggac aatttcaacg tctttttctc
     2941 ttttccttgt tcttttgcca aattcttgtt ttgttgtgaa caaaagacaa catttcattt
     3001 ttcatgagac cttgtcctgt actttctgca caaaaataat ttttttcgtg ccccctgctg
     3061 cattgacttt gcattttttc cgcggtatag tagggattgg aattgcgggt gtcgtttttg
     3121 agaggacagt tttgcggcaa catcactgtc ccctgccccc cggcagcccg gcccgcaggc
     3181 gccggaaaat tttgtgatgg aggaattttt gtgaagaaga tctggagctc gtacaaatcc
     3241 tcgttcatcc tgttgggctg catggtgctc ggcggcatcg tgggcgcgtt ctggggcccg
     3301 ggcgccagcg tgctgacccc ctgggcgaac atctttttga acctgctgta ctgcgtggtg
     3361 gtgccgatga tctttgtctc gctggtctcg gccattgccg gtatgcagaa catgcgcaag
     3421 ctgggcaagc tgctggcggt gctgctggtg ctgtttttga tcaccggcat cgtgtgcagc
     3481 gcctacatgg tgggcttttc ggccctgttt gacccggcca agaacaccga tatgacccag
     3541 ttcacc
//
LOCUS       DBMDYA010000246         3542 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6028_length_3542_cov_1.446963, whole genome shotgun sequence.
ACCESSION   DBMDYA010000246 DBMDYA010000000
VERSION     DBMDYA010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_3542_cov_1.446963"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="ACZPFK_06995"
     CDS             complement(<1..1092)
                     /locus_tag="ACZPFK_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015662.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR1406092.1"
                     /translation="MKQQGHFKKRAVILLALVALVFGGFAVKLFQLQIVDGAEYLDQA
                     TSTTMITVPLPAARGEIVDRYGRAIATNRAAYNLWLIRALLPDDQLNDTLMRMVDTLG
                     ENGEEWNDDAPLTHSAPYEFVEGRDADVARMKTALGLAQYATAQNVYDKMLEAYDLTG
                     LPEKYQRILAGIRYQMQYEEYSNVTPFVLATDVSLKTVSTVKENNLDLPGVYVAEGTV
                     RVYTDPTLMPHIIGTIGKIYAEEWESTYKNQKNYKMNDLVGKSGIELAYESTLKGTDG
                     QMQIELTRTGSVVATTVVEPVPGDTVVLTVDKNLQADCQKILENQVAALKAAAAANRE
                     GDPEGASLVVLDVKTGGVLAAATYPSYDLN"
     gene            complement(1138..1659)
                     /locus_tag="ACZPFK_07000"
     CDS             complement(1138..1659)
                     /locus_tag="ACZPFK_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1406093.1"
                     /translation="MDARKRMLLQVARHLCYALSLLAAYVLQTTPGFLTFGGVKPVLL
                     VPLALCIGVFEGAFTGALYGAAAGLMWDIASGRTGGFFAIMLMIFCFVCAVSVALYLR
                     GTILNVSLLTGGVMLLMCSADFLFGYLLHGYSGLGRLYLTRILPVVVFSAAVTFAPWL
                     LVRKISEKFVPGE"
     gene            complement(1670..2503)
                     /gene="mreC"
                     /locus_tag="ACZPFK_07005"
     CDS             complement(1670..2503)
                     /gene="mreC"
                     /locus_tag="ACZPFK_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1406094.1"
                     /translation="MKDFFSSLKFKILVGVLVLLAGVMTYAAANGRLSAAPQELLGAI
                     AVPFQRISAAISGAAEGFVDKYIRIDSIIEENEQLQQENAQLREQLVDYDIMKRENEQ
                     LREAWEMKEEYPDRQIVNANVIGRDPLEKYYSFTIDKGERHGVKVKDCVITAKGVVGR
                     VIEVGPNYAKVATILDPNVFISCMTSRTGDIGLVGGDVTLSQGKQCAMTLLPRDTMAA
                     AGDEVITTNEGGAFPKGLAVGRVAEVLPEASGKSMVAVVDPYEDIESVKMVMVITEFS
                     E"
     gene            complement(2628..>3542)
                     /gene="mreB"
                     /locus_tag="ACZPFK_07010"
     CDS             complement(2628..>3542)
                     /gene="mreB"
                     /locus_tag="ACZPFK_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1406095.1"
                     /translation="ETKTDTVRYVGHEAKEVIGRTPGSIVAVRPLKEGVIADFDIASS
                     MLQIFIKKACGSSMFSRPRVVICIPSGVTEVERRAVREASLKAGARQVMVIEEPMAAA
                     IGAGLPVSEATGSMVVDIGGGTSEVAVISLGGIVASRSVRVGGDAFDAAIIAYIKRKY
                     NLLIGERTAELIKIEIGSAYKLEEEGSLDIKGRNLVDGLPKNITVHSEEIREALDEPL
                     HRIIDAIKETLERTPPELAADIIDHGITLTGGGALLRGLDQLVGSYTGIDVHVAETPL
                     DCVATGAGLVLDNMDLLAEVVADAERKF"
BASE COUNT          570 a         1201 c         1115 g          656 t
ORIGIN      
        1 gttcaggtcg tagctggggt aggtggccgc ggccagcacg ccgccggtct tcacgtccag
       61 caccaccagc gaggcgccct ccgggtcgcc ctcgcggttg gcggccgcgg ccgctttcag
      121 ggccgccacc tggttttcca aaattttctg gcaatcggcc tgcaggtttt tgtccaccgt
      181 gagcaccacc gtgtcgccgg gcaccggctc caccacggtg gtggccacca cgctgccggt
      241 gcgggtgagc tcgatctgca tctggccgtc ggtgcctttg agggtgctct cgtaggccag
      301 ctcaatgccg ctcttgccca ccaggtcgtt catcttgtag ttcttctggt ttttgtaggt
      361 gctctcccac tcctcggcat agatcttgcc gatggtgccg atgatgtggg gcatcagggt
      421 ggggtcggtg tagacccgca ccgtgccctc ggccacatag acgccgggca ggtccaggtt
      481 gttctccttt acggtggaga cggtcttcag cgagacgtcg gtggccagca caaagggggt
      541 gacgttgctg tactcctcgt actgcatctg gtagcggatg cccgccagga tgcgctgata
      601 cttttccggc aagccggtca ggtcgtaggc ctccagcatc ttgtcataga cgttctgagc
      661 ggtggcgtac tgggccaggc ccagggcggt cttcatgcgg gccacatccg cgtcccgccc
      721 ctcgacgaac tcgtagggcg ccgagtgggt cagcggggcg tcgtcgttcc actcctcacc
      781 gttctccccc agggtgtcca ccatgcgcat cagcgtgtcg ttgagctgat cgtccggcag
      841 cagcgcgcgg atcagccaca ggttgtaggc cgcccggttg gtggcgatgg cccggccgta
      901 gcggtcgacg atctcgcccc gcgcggcggg cagcggcacg gtgatcatgg tggtggaggt
      961 cgcctggtcc aggtactcgg ccccgtccac gatctgcagc tgaaaaagct tgaccgcaaa
     1021 gccgccaaag accagggcca ccagcgccaa aaggatgacc gcccgctttt taaaatgtcc
     1081 ctgctgtttc atctcgggcc gcgccctcct ctcttctgtt gtgtccggcc gggcgggcta
     1141 ttccccgggg acgaattttt cggatatctt gcgcaccagc agccagggcg caaaggtgac
     1201 ggcggcggag aacaccacca ccggcaaaat gcgggtgagg tagagcctgc ccagcccgct
     1261 gtacccgtgc agcagatagc cgaacagaaa atccgcgctg cacatcagca gcatcacgcc
     1321 cccggtgagc agcgagacgt tgaggatggt gccgcgcaga taaagcgcca ccgagacggc
     1381 gcacacaaag caaaaaatca tcagcatgat ggcaaaaaag ccgccggtgc ggccggaggc
     1441 gatgtcccac atcagccccg ccgccgcgcc gtagagcgcg ccggtgaaag ccccctcaaa
     1501 caccccgatg cacagggcca gcggcaccag cagcaccggc ttgaccccgc cgaaagtgag
     1561 aaagcccggc gtggtctgca gcacataggc cgccagcagg ctcagggcat agcagagatg
     1621 ccgcgccacc tgcaaaagca tccgttttct cgcgtccatg ccccgccccc tactcgctga
     1681 actcggtgat gaccatcacc atcttgacgc tctcgatgtc ctcgtagggg tcgaccaccg
     1741 cgaccatcga cttgccgctg gcctcgggca gcacctcggc cacccggccc accgccagcc
     1801 ctttggggaa cgccccgccc tcgttggtgg tgatcacctc gtcgccggcg gcggccatgg
     1861 tgtcccgggg cagcagggtc atggcgcact gcttgccctg cgagagggtc acgtcgccgc
     1921 ccaccaggcc gatgtccccg gtgcggctgg tcatgcagct gatgaagacg ttggggtcca
     1981 ggatggtggc cacctttgcg tagttggggc ccacctcgat cacccggccc accacgccct
     2041 tggccgtgat gacgcagtcc ttgaccttta ccccgtgccg ctcgcccttg tcgatggtga
     2101 acgagtagta tttttccagc gggtcgcggc cgatgacgtt ggcgttgacg atctggcggt
     2161 cggggtactc ctctttcatc tcccaggcct cgcgcagctg ctcgttctcc cgtttcatga
     2221 tgtcgtagtc caccagctgc tcccgcagct gggcgttctc ctgctgcagc tgctcgttct
     2281 cctcgatgat cgagtcgatg cggatatatt tgtccacaaa gccctcggcc gcccctgaga
     2341 tggcggccga gatgcgctga aagggcaccg cgatggcgcc cagcagctcc tgcggggcgg
     2401 ccgagaggcg gccgttggcc gcggcatagg tcatcacgcc ggccagcagc accagcaccc
     2461 cgaccagaat cttgaatttg agggaagaga aaaaatcttt cacgcggtct cggcctcatc
     2521 tcgttgaaaa taggaaaaaa cagctgcccg ccgccgcgga caagtaccac aaaagcggca
     2581 gagggcgggt ggccccgccc cttctgccgc tttttgcatt tcccggatca gaacttgcgc
     2641 tcggcgtcgg ccacgacctc cgccagcagg tccatattgt ccagcaccag cccggcgccg
     2701 gtggccacgc agtccagcgg cgtctcggcc acatgcacgt cgatgcccgt atagctgccc
     2761 accagctggt ccaggccgcg cagcagcgcg ccgccgccgg tgagggtgat gccgtggtcg
     2821 atgatatcgg ctgccagctc gggcggggtg cgctccagcg tctccttgat ggcgtcgatg
     2881 atgcggtgca gcggctcgtc cagcgcctcg cggatctcct cgctgtgcac ggtgatgttc
     2941 ttgggcaggc cgtccaccag gttgcgcccc ttgatgtcca ggctgccctc ctcctccagc
     3001 ttgtaggccg agccgatctc gatcttgatc agctctgcgg tgcgctcgcc gatgagcagg
     3061 ttgtacttgc gcttgatata ggcgatgatg gcggcgtcga acgcgtcgcc gcccacccgc
     3121 acgctgcggg aggccacgat gccgcccagg ctgatgacgg ccacctcgct ggtgccgccg
     3181 ccgatgtcca ccaccatgct gccggtggcc tccgacacgg gcaggcccgc gccgatggcg
     3241 gccgccatcg gctcctcgat gaccatgacc tggcgggcgc ccgccttgag gctggcctcg
     3301 cgcacggcgc ggcgctccac ctcggtgacg ccggagggga tgcagatgac cacccgcggg
     3361 cggctgaaca tgctgctgcc gcaggccttt ttgatgaaga tctgcagcat gctggacgcg
     3421 atgtcgaaat cggcgataac gccctccttc agcgggcgca ccgcgacgat gctgcccggg
     3481 gtgcggccga tgacctcttt ggcctcatga cccacatacc gcaccgtgtc ggtcttggtt
     3541 tc
//
LOCUS       DBMDYA010000247         3541 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6030_length_3541_cov_1.555814, whole genome shotgun sequence.
ACCESSION   DBMDYA010000247 DBMDYA010000000
VERSION     DBMDYA010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_3541_cov_1.555814"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="ACZPFK_07015"
     CDS             <1..518
                     /locus_tag="ACZPFK_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1406096.1"
                     /translation="RKIKNRQIPYMRRSLGVVFQDFRLIPTMTAYENVAFAMRVTNIP
                     EKQIRSRVPYVLNLVGLKDKARSYPPELSGGEQQRVALARAMVHSPPIIIADEPTGNI
                     DPELSVEIMELLTAINKVGTTVVVVTHEQELAAQFHQRTIYIEKGEVVADTSAGLRDK
                     LVAPSEVEVAR"
     gene            515..1438
                     /gene="ftsX"
                     /locus_tag="ACZPFK_07020"
     CDS             515..1438
                     /gene="ftsX"
                     /locus_tag="ACZPFK_07020"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1406097.1"
                     /translation="MSLSGFKYLTKQGLHNLTRNKLMSIASIGVLTACLVITGVAALL
                     SVNVGNFVDYLGAQNKVIVYMQPDADEAAVQAADAAIKAMPNILSYTYVDKDEAIEEM
                     QGWMSDGDGESYGELLEPYKGDGNPLLASFRVTVDDLTQMDATVAQLKALPGLYTVDA
                     PSDLAGTLVSLKNTVNIAGWGLVAVLVVVSLVVISNTIRITVFARRKEINIMKFVGAT
                     NGFIRLPFLVEGTAIGLISAVLAFGLVSGAYLGVLEAVSRSGAGLLSNLYFTLLSYRS
                     VWAPLLIGFVGSGAVLGGFGSAASVRKHLKV"
     gene            1479..2630
                     /locus_tag="ACZPFK_07025"
     CDS             1479..2630
                     /locus_tag="ACZPFK_07025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1406098.1"
                     /translation="MKKKTARIICLAMAILMALMLALPYVARATAADDVQRLQQELDE
                     VKKKGRELEEKEEQQKSYKANLQKQNDIIQKQIEALGVSIDQTKEALAQKQAELDQKR
                     RDIAETDALFQERLRAMQVNRTSGLLSTLLAVNSFDELLTASTTLSRISAADTDLLKK
                     LAAEKAEMERQEAEINAQLEQLQADLEAQNAKKQELAGNIKAVDASLTDLEAEQQANA
                     ADQARLTKEYNAARAAAQAEMGKPSDNPDFVGGSYAWPVPGFYNISSPYGWRILYGQR
                     DFHTGIDISGGGRVIYGAAVTASLPGTVQTVVYGSRGYGYYVIVDHGGNNKTLYGHMS
                     AIYVTPGQAVGQGDAVGAVGSTGNSTGPHLHFEIRQNGQQVDPAPFVGR"
     gene            2866..>3541
                     /locus_tag="ACZPFK_07030"
     CDS             2866..>3541
                     /locus_tag="ACZPFK_07030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1406099.1"
                     /translation="MKKKISVSLAIAIAIIAMTVTFSVTMILAMKLFDRTVSDVNEKQ
                     VMYNKLAEIDKVARDNFYTEINDQTLYDMLATGYVAGLGDRNSRYYTAKQVTELNDTA
                     SGKLMGIGVEVVKDASGYFRIVKIYPGSPAEAAQLSQNLIVTKLDETDLKTLTLDAVN
                     SLLKGESGTTIKVTYLLDNAETPVEIQRSPFDMPTVEFQLVDTVGYIKIRTFANETVS
                     KLDYAVN"
BASE COUNT          715 a         1113 c         1157 g          556 t
ORIGIN      
        1 tccgcaagat caaaaaccgg cagatcccct acatgcgccg ctcgctgggc gtggtctttc
       61 aggatttccg gctcatcccc accatgaccg cctacgagaa cgtggccttt gccatgcggg
      121 tgaccaacat ccccgaaaag cagatccgca gccgggtgcc ctatgtgctc aacctggtgg
      181 gcctcaagga caaggcgcgc agctacccgc ccgagctctc cggcggcgag cagcagcggg
      241 tggcgctggc ccgggccatg gtgcactcgc cgcccatcat catcgcggac gagcccaccg
      301 gcaacatcga ccccgagctg tcggtggaga tcatggagct gctcaccgcc atcaacaagg
      361 tgggcaccac ggtggtggtg gtcacccacg agcaggagct ggccgcccag ttccaccagc
      421 gcaccatcta catagaaaag ggcgaggtcg tggccgacac cagcgccggg ctgcgggaca
      481 agctggtggc ccccagcgaa gtggaggtgg cgcgatgagc ctttccggtt tcaaatatct
      541 gacaaagcag ggcctgcaca acctgacccg caacaagctg atgagcatcg cctccatcgg
      601 cgtgctcacc gcctgcctgg tcatcaccgg cgtggcggcg ctgctgtcgg tgaacgtggg
      661 caattttgtg gattatctgg gcgcccagaa caaggtcatc gtctacatgc agcccgacgc
      721 cgacgaggcg gccgtgcagg ccgcggacgc ggcgatcaag gccatgccca acattctgag
      781 ctatacctat gtggacaagg acgaggccat cgaggagatg cagggctgga tgtccgacgg
      841 cgacggcgag tcgtacggcg agctgctgga gccctataag ggcgacggca acccgctgct
      901 ggccagcttc cgggtgacgg tggacgacct gacccagatg gacgccacgg tggcccagct
      961 caaggcgctg ccgggccttt acacggtgga cgcgcccagc gacctggccg gcacgctggt
     1021 ctcgctcaaa aacacggtca atatcgctgg ctgggggctg gtggcggtgc tggtggtggt
     1081 ctcgctggtg gtgatctcca acaccatccg catcaccgtt ttcgcccgcc gcaaggagat
     1141 caacatcatg aagttcgtgg gcgcgaccaa cggcttcatc cggctgcctt ttctggtgga
     1201 gggcacggcc atcggcctga tctcggccgt gctggccttt gggctggtgt ccggggccta
     1261 tctgggcgtg ctggaggcgg tctcccgctc cggcgccggg ctgctgtcca acctctactt
     1321 caccctttta tcctaccgct cggtctgggc cccgctgctg atcggctttg tgggcagcgg
     1381 cgcggtgctg ggcggcttcg gcagcgcggc cagcgtgcgc aagcatttaa aggtctgagg
     1441 ggcgggcagg caaaagacca ctgcattcgg aggcgcgtat gaaaaaaaag actgcacgca
     1501 ttatctgcct tgccatggcc atcctcatgg ccctgatgct ggccctgccc tatgtggcgc
     1561 gggcgacggc ggccgacgac gtacagcggc tgcagcagga gctggacgag gtcaagaaaa
     1621 agggccggga gctggaggag aaagaggagc agcagaagtc gtacaaggca aacctgcaaa
     1681 agcagaacga catcatccaa aagcagatcg aggcgctggg cgtctccatc gaccagacca
     1741 aggaggcgtt ggcccaaaag caggccgagc tggaccagaa gcggcgcgac atcgccgaga
     1801 ccgacgccct gtttcaggag cggctgcggg ccatgcaggt gaaccgcacc tccggcctgc
     1861 tgtcgaccct gctggcggtc aacagctttg acgagctgct caccgcctcc accaccctct
     1921 cgcgcatctc ggcggccgac accgacctgc tgaaaaagct ggcggcggaa aaggcggaaa
     1981 tggagcgcca ggaggcggag atcaacgccc agctggagca gctgcaggcc gatctggagg
     2041 cccagaacgc caaaaagcag gagcttgccg gcaatatcaa ggcggtggac gccagcctga
     2101 ccgacctgga ggcggagcag caggccaacg ccgccgatca ggcccggctg accaaggagt
     2161 ataacgccgc ccgcgcggcg gcgcaggcgg agatgggcaa gccctcggat aatccggatt
     2221 ttgtgggcgg cagttacgcc tggccggtgc ccggctttta caatatctcc tcgccctatg
     2281 gctggcgcat cctctacggg caacgggatt tccacaccgg catcgacatt tccggcggcg
     2341 gccgggtgat ctacggcgcg gcggtgaccg ccagcctgcc gggcacggtg cagacggtgg
     2401 tctacggctc gcgcgggtac ggctattacg tcatcgtgga ccacggcggc aacaacaaga
     2461 ccctttacgg ccacatgagc gccatctatg tcacgccggg gcaggcggtg ggccagggcg
     2521 acgcggtggg cgcggtgggc agcaccggca actccaccgg cccgcacctg cactttgaga
     2581 tccgccagaa cggccagcag gtggacccgg ccccgtttgt ggggcgctag gcgttcataa
     2641 aaccaacaca ttccggcaat ataatgaggc ggaatcggcg gcgcggcccc gttgcgccgc
     2701 accgcaaaaa aacggacaag gccgccgcgc accccggggg cctgcgcggc ggctgcgctg
     2761 tgcgggcccc ggggcgcgtt ttgccggctt gtccgaaccc ggctttgggg gcaagggccc
     2821 ctttgcccct gaaaaaagtg ccgagatatc cgggaggttc gcaagatgaa gaaaaagata
     2881 tcggtcagcc tcgccatcgc catcgcgatc atcgccatga ccgtgacctt ttcggtgacc
     2941 atgatcctgg ccatgaagct gtttgaccgg accgtcagcg acgtgaatga aaaacaggtg
     3001 atgtacaaca agctggcgga gatcgacaag gtggcgcggg acaactttta caccgagatc
     3061 aacgaccaga ccctgtacga catgctggcc accggctatg tggccggcct gggcgaccgc
     3121 aacagcagat attacaccgc caagcaggtg accgagctca acgacacggc cagcggcaag
     3181 ctgatgggca tcggcgtcga ggtggtcaag gacgccagcg gctatttccg catcgtcaag
     3241 atctatccgg gcagccccgc cgaggcggcg cagctctcgc agaacctgat cgtcaccaag
     3301 ctggacgaga cggacctgaa gaccctcacg ctggacgcgg tgaacagcct gctgaaaggg
     3361 gagagcggca ccaccatcaa ggtgacctat ctgctggaca acgccgagac gccggtggag
     3421 atccagcgca gcccctttga catgcccacg gtggagtttc agttggtgga cacggtgggg
     3481 tatatcaaga tacgcacctt tgccaacgag acggtgtcca agctggacta tgccgtcaac
     3541 g
//
LOCUS       DBMDYA010000248         3526 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6060_length_3526_cov_0.862190, whole genome shotgun sequence.
ACCESSION   DBMDYA010000248 DBMDYA010000000
VERSION     DBMDYA010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6060_length_3526_cov_0.862190"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /locus_tag="ACZPFK_07035"
     CDS             <1..2193
                     /locus_tag="ACZPFK_07035"
                     /inference="COORDINATES: protein motif:HMM:NF039380.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1406100.1"
                     /translation="GTTLFGNLAPLGVALVAGVPQGGLLAATAGAVVGSAISLPFSMS
                     GKYLAGLLAAGAVRLLLTRVKARLDPAAASSLLGAAALLAAQAVYTLRLGEGPLAILN
                     TVSEGLLAIGMAVMFSIALPPLAAGRHPLSQPGAVQGAAVICAGAALLGLGRIQLLGL
                     NLGCVTLGVLLLALALSGAGRAAVMALAGTTMLYLGAPADAWMGLGLGAAGLAAAAFR
                     DRGRPAMAGCFFAASCLGLFGAPEPLPGLWYLVGAGCSALIFLALPVSVLEPVLEPGE
                     NAVSEAVLTSHVSARLYALSEALCQVGATVEEVCRHLPGPQITLNDLYDRVAEGPCAH
                     CPARTNCWVAHCSDTMDGLTSVKEALSAGREITRETLPGPLAALCVRPAVLAGAISQY
                     YADYLTRRTVAARTGVLRSALTQQYTAVADALGQVAGELFEETVLDNRRAGKVSDLFY
                     AVGLPPLDVAAVLTRDGRCRVTVKTGRASLSDEALGELCTEVSACCGRRFSCTRHTAR
                     GAASQFEFYEAAPYEVQFATAGRSAAGGVSADAVKTFCDVRGRAHMILCDGMGTGKAA
                     AVDGVLAATLSRQLIDAGFEGASAARLVNVALSLKGEDAGATLDLLTVDLYTGRCDLF
                     KAGAAPTYVVKRGRAVALATESLPVGILGGVVGRSAHTALGDGSMAVMVSDGAIAAGD
                     EWLKAELERLAAEEAKTVADALLEGAVRRETGHRDDITVSVLRLDRRE"
     gene            2995..3378
                     /locus_tag="ACZPFK_07040"
     CDS             2995..3378
                     /locus_tag="ACZPFK_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1406101.1"
                     /translation="MNAKLSAAELEVMRHVWAAEGPVTCEALRASLAGEREWKTTTVL
                     TFLSRLADKGMLTVTKRGRANLYAPAVSRRQYEGSETAQFLEEMHGGNVKRMVASLCE
                     AGRLAPGELEELRAWLEAQGGGERG"
BASE COUNT          486 a         1117 c         1337 g          586 t
ORIGIN      
        1 ggcaccacgc tgttcggcaa cctggccccg ctgggggtgg cgctggtggc gggcgtgccg
       61 cagggcggcc tgctggccgc cacggcgggg gcggtggtgg gcagcgccat cagcctgccc
      121 ttttcgatga gcggcaaata cctggccggc ctgctggcgg cgggcgccgt acgactgctg
      181 ctgacccggg tcaaggcccg gctggacccc gcggccgcct cgtcgctgct gggggcggcg
      241 gcgctgctgg cggcgcaggc ggtctatacg ctgcggctgg gcgaggggcc cctggccatc
      301 ctcaacaccg tctcagaggg actgctcgcc atcggcatgg cggtgatgtt ctccatcgcc
      361 ctgcccccgc tggccgcggg tcggcatccg ctctcgcagc cgggggcggt gcagggtgcg
      421 gcggtgatct gcgccggggc ggcgctgttg gggctggggc gcatccagct gctggggctg
      481 aacctgggct gtgtgacgct gggagtgctg ctgctggcgc tggccctctc cggcgccggc
      541 cgggcggcgg tgatggccct ggccgggacc accatgctct atctgggcgc cccggctgac
      601 gcctggatgg gcttagggct gggggcggcc gggctggcgg cggccgcttt tcgggaccgg
      661 ggccgcccgg cgatggcggg ctgttttttc gccgcctcct gcctgggcct gttcggcgcg
      721 cccgagccgc tgcccggcct ctggtatctg gtgggggcgg gatgctctgc cctaatcttt
      781 ctggcgctgc cggtctcggt gctggagccg gtgctggagc cgggggaaaa cgccgtcagc
      841 gaggccgtgc tgaccagcca cgtctcggcc cggctctacg cgctcagcga ggcgctgtgc
      901 caggtgggcg ccacggtgga ggaggtatgc cgccatttgc ccggcccgca gatcaccctc
      961 aacgacctgt acgaccgggt ggccgagggg ccctgcgccc actgcccggc ccgcaccaac
     1021 tgctgggtgg cccactgcag cgacaccatg gacggcctca cgtcggtcaa ggaggcgctg
     1081 agcgccgggc gggagatcac ccgcgagacc ctgcccggcc cgctggcggc cctctgcgtg
     1141 cggccggcgg tgctggccgg ggcgatcagc caatactatg ccgattacct cacccggcgc
     1201 accgtggccg cccgcaccgg cgtgctgcgc agcgccctga cccagcagta cacggcggtg
     1261 gccgacgcgc tggggcaggt ggcgggcgag ctgttcgagg agacggtgct ggacaaccgg
     1321 cgcgcgggca aggtgagcga cctgttttac gcggtgggcc tgccgccgct ggatgtggcg
     1381 gcggtgctca cgcgggacgg gcgctgccgc gtgacagtca agacggggcg ggccagcctg
     1441 tccgacgagg cgctcgggga gctctgcact gaggtctcgg cctgctgcgg acgcaggttt
     1501 tcctgcacgc ggcacaccgc ccgcggcgca gccagccagt tcgaatttta tgaggcggca
     1561 ccctatgagg tgcagttcgc caccgccggg cgcagcgcgg cgggcggcgt ttccgccgac
     1621 gcggtcaaaa ccttttgcga cgtgcgcggc cgcgcgcaca tgatcctctg cgacggcatg
     1681 ggcactggca aggccgcggc ggtggacggc gtgctggccg ccaccctcag ccgccagctc
     1741 attgacgcgg gctttgaggg cgcgtcggcc gcccgtctgg tcaacgtggc cctctccttg
     1801 aaaggggagg acgcgggcgc aacgctggac ctgctcacag tggatctcta caccgggcgc
     1861 tgcgacctgt tcaaggccgg ggccgcgcct acctatgtgg taaagcgggg ccgggcagtg
     1921 gcgctggcca ccgagagcct gccggtgggc atcctgggcg gggtggtggg ccgcagcgcc
     1981 cacaccgcgc tgggggacgg ctcgatggcg gtcatggtca gcgacggcgc catcgccgcc
     2041 ggggacgagt ggctcaaggc ggagctggag cgcctggcgg cggaggaggc gaaaacggtg
     2101 gccgacgcgc tgctggaggg cgcggtgcgc cgggagaccg gccaccggga cgacatcacg
     2161 gtctcggtgc tgcggctgga ccgccgcgag tgagcggctg cgggcaagaa aagacgactg
     2221 ttttgacgcc gcgccgcagg gacgtgtgtg cgcaggggga cgttgccccg gcagataggg
     2281 gaaagggatc tgcgcgcggt gctttgtccg gcccgtcccc cggccagaga gggctctgcg
     2341 atttcgcccc gcgaaatgcc gacaccggcg ggcaatccgc ccaggttttt ccggcgctgc
     2401 cccgagccct tttctcccgg cggcgcgcag cggtttcaaa gcggctgtgg tgacgccgcg
     2461 agagctttaa aaaatagccg ctgtgcggct atttttttgg ccatccggcc agtcctctgg
     2521 ccggggccat taggattagt tgttttttat tgcgaagcaa taaaaaatag ccgctgtgcg
     2581 gctatttttt ttggccatcc ggccaatccc ttggccgggg ccattaggat cagttgtttt
     2641 ttattgcaaa gcaataaaaa ccagattcaa aagggctggc gatccgcagg ggagcgccgg
     2701 ccctttttgc acggtacttt tccagggccg ccgtccccaa gccgatgctt tccctccgca
     2761 gcgcgcactt gaacgcagtg ccgctccggc gcggggcgat gggggagaaa gggggaggga
     2821 cagcgcgcgt cctcctctcc atccagcttg atggggacag gtccggccgc cggtcgtcgg
     2881 ccgggaggaa agtttcacat ccgggtgctt gacagaaagt tctacatgat gtagtatata
     2941 attactacaa aatgtagaag caagaggttg ctcttgaaca aagaggtgaa agccatgaat
     3001 gcgaagctga gcgctgcgga gctggaggtc atgcggcatg tctgggcggc agaggggccg
     3061 gtcacctgcg aggcgctgcg ggcgtccctg gcgggcgagc gggagtggaa gaccaccact
     3121 gtgctgacct ttctctcccg tctggccgac aaggggatgc tcaccgtgac caagcggggg
     3181 cgggccaatc tctacgcccc ggccgtgagc cggcggcagt atgagggcag cgagacggcg
     3241 cagtttttag aggagatgca cggcggcaac gtcaagcgga tggtggcctc gctgtgcgag
     3301 gccgggcggc tcgcacccgg cgagctggaa gaattgcggg cctggctgga ggcccagggg
     3361 ggaggggagc gcggatgatg cggtttttct tcgattggat gctgcccgcc tcgctggcgg
     3421 gcggcgcctg tgcgctgctg ctctttgcgc tgcgcccctg gctcacttcg gcgccgaaac
     3481 gggcggctgc tgccatcgcg gtgtgcaccg tgctggcggc cctgga
//
LOCUS       DBMDYA010000249         3521 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6064_length_3521_cov_1.776316, whole genome shotgun sequence.
ACCESSION   DBMDYA010000249 DBMDYA010000000
VERSION     DBMDYA010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_3521_cov_1.776316"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="ACZPFK_07045"
     CDS             <1..687
                     /locus_tag="ACZPFK_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1406102.1"
                     /translation="GSVLNQVLLHQSIIGLEAKQAMDLLGEYPDVIIGCAGGGSNLGG
                     LIAPYMRDKLTGAADPQIVAVEPASCPSLTRGRYAYDFCDTGRITPLAKMYTLGSGFI
                     PSPGHAGGLRYHGMSPILSKLYHDGYLSAVSVEQTRVFEAATFFARQETILPAPESAH
                     AIRAAMDEALRCRESGEAKTILFGLTGTGYFDMTAYTAFLEGKMADTVPTDAELQRGF
                     DSLPQVPGQN"
     gene            complement(1008..1817)
                     /locus_tag="ACZPFK_07050"
     CDS             complement(1008..1817)
                     /locus_tag="ACZPFK_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406103.1"
                     /translation="MLGKLLKYELRATGRQVLPLCGAMVLYAALCRLLGALRITRPWP
                     RLDEALASIGTFFFILLLFACCVASNIVLVVRYYRNLYGDEGYLMNTLPVTPAQNIWA
                     KLISAFLWEMAVALSVFVSLILLLAGTPLFPTIGRELGWVLSLVSRAFALHPGSIFLG
                     VAEVLLLVPVSALSALLMLYAAISLGQFIRRHRVLGAVAFYFVLDFAFGVVTTLLFLF
                     LGLLGTGWLQTVSPYSFLLLMWGGILLMIAAQGVVCFGISRWCLSKKLELE"
     gene            complement(1811..2509)
                     /locus_tag="ACZPFK_07055"
     CDS             complement(1811..2509)
                     /locus_tag="ACZPFK_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406104.1"
                     /translation="MEPILECRQLFKDYTSCRALAGVDLTLYRGRIVGLLGPNGSGKT
                     TLIKLAAGLLAPTAGEILVAGHTPGPETKALVSYLPERTYLPDWMTARQVIGFFADFY
                     TDFDSAAAFDMLQRLGVLADAKLKTLSKGTKEKVQLILVMSRRAQLYLLDEPIGGVDP
                     AAREYILNTILTNYNANATVLISTHLISDIEKVLDQVVFLHAGQVDLCATVDDIRAER
                     GCSVDTFFREVFKC"
     gene            complement(2559..2957)
                     /locus_tag="ACZPFK_07060"
     CDS             complement(2559..2957)
                     /locus_tag="ACZPFK_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406105.1"
                     /translation="MNWDLKSDRPIYIQLVEHLQLAIISGLLPPGSRVPPVRELAAEA
                     AVNPNTMQKALQELEQGGLVQTQRTSGRTVTQDTGRIAAVKAEFAGGEVSAFLEKMGR
                     LGYAPQQAVALVEEAARQAGTDGAGGTRQA"
BASE COUNT          710 a         1121 c         1064 g          626 t
ORIGIN      
        1 ggctcggtgc tcaaccaggt actgctgcat caatccatca tcggcctgga ggccaaacag
       61 gcgatggatc tgctggggga ataccctgac gtaatcatcg gctgcgcggg cggcggctcc
      121 aacctgggcg gcctgatcgc cccctatatg cgggataagc tcaccggcgc ggcggacccg
      181 cagatcgtgg cggtggaacc ggcctcctgc ccctccctca cccgcggccg ctatgcctac
      241 gacttttgcg acaccggccg catcaccccg ctcgccaaga tgtacacgct gggcagcggc
      301 ttcatcccct cccccggtca tgcgggcggg ctgcgctacc acggcatgtc ccccatcctc
      361 tccaagctgt accacgacgg ctatctgagc gctgtctcgg tagagcagac ccgcgtcttt
      421 gaggcggcca ccttttttgc ccgccaggag accattctgc ccgcgcccga gtccgcccac
      481 gccatccgcg ccgccatgga cgaggcgctg cgctgccgcg agagcggcga ggcaaagacc
      541 atcctgttcg gcctgaccgg taccggctat ttcgacatga ccgcttacac cgcatttctg
      601 gaggggaaga tggccgacac cgtgcccacc gacgcggagc tgcagcgcgg cttcgacagc
      661 ctgccccagg tgcccggcca gaactgagcc aataggccta ccggcgcccc cacaaggcgg
      721 gggcgggctg ggccctgctc tttgtgcaaa gtcttctctt ttgcccggcg ccaggcggcc
      781 gctgtccggc tcctctttca atggagccgt cacgcaggcg ccggggggta tctgcggcag
      841 tttatttata tccgaaagaa aaaagcttgg ttcaccaagc cggcgcctct gctctccgcg
      901 gccgctttcg cccgatggcc ctgtttcccg ccgctgcgcg gcaactgaaa caggtggcaa
      961 aaaaaatagc cgcaaagcgg ctattttttt gcttgcgtcg ttcactgcta ttccagctcc
     1021 agctttttcg acaggcacca gcggctgatg ccaaagcaga ccacgccctg ggccgcgatc
     1081 atcagcagga tgccgcccca catcaacagc agaaaagaat agggggacac ggtctgcagc
     1141 cagcctgtgc ccagcaggcc gagaaagaga aagagcaggg ttgtgacgac gccgaacgca
     1201 aaatccagca caaagtagaa cgccaccgca cccagcacac gatgccggcg gatgaattgc
     1261 cccagcgaga tggcggcata aagcatcagc agtgcggaaa gggcggacac cgggaccagc
     1321 agcagtactt cggcaacacc cagaaagata ctgcccgggt ggagggcaaa tgcccggctc
     1381 accagggaca atacccagcc caactcgcgc ccgatggtgg ggaacagggg ggtgccggcc
     1441 agcagcagga tcaacgagac gaacaccgag agcgccaccg ccatctccca caaaaaggcg
     1501 gagatcagct tggcccagat gttttgggcc ggggtcaccg gcagggtatt catcagataa
     1561 ccctcgtcgc cgtagagatt gcggtaatac cgcaccacaa ggacgatgtt gctggcgacg
     1621 cagcaggcga agagcagcag gatgaaaaag aaagtgccga tgcttgccag cgcctcgtcc
     1681 agccggggcc acggacgggt gatgcgcagc gcgcccagca ggcggcagag cgcggcgtaa
     1741 aggaccatgg ctccgcaaag cggcagaacc tgccgcccgg tggcccgcag ttcatacttc
     1801 agcagtttgc ctaacatttg aatacctccc ggaaaaacgt gtccacgctg catccgcgct
     1861 cggctcggat gtcatccacc gtggcgcaga ggtccacctg accggcgtgc agaaagacca
     1921 cctggtccag cactttttca atgtcgctga tcaggtgggt ggaaatcagc accgtggcgt
     1981 tggcgttgta gttggtgagg atcgtgttca ggatgtattc ccgtgccgcc gggtccaccc
     2041 cgccgatcgg ctcgtccaga agatagagct gggcgcgccg gctcatcacc aaaatcagct
     2101 gcaccttttc tttggtgccc ttggagaggg ttttcagctt ggcatccgcc agtacgccga
     2161 gccgctgcag catgtcaaag gcagcggcgg agtcaaaatc agtgtaaaaa tcggcgaaaa
     2221 agccgatgac ctgccgggcg gtcatccagt cgggcaggta ggtgcgctcc ggcagatagg
     2281 acaccagcgc ttttgtttca ggccccggcg tgtggcccgc gactaagatc tcgccggccg
     2341 tgggcgccag caggcctgcc gccagcttga tcagggtggt cttgccgctg ccgttggggc
     2401 cgagcagccc cacgatcctg ccacggtaga gggtcaggtc cacgcccgcc agcgcgcggc
     2461 aggacgtata gtctttgaac agctgcctgc actctaaaat aggttccata aaaacaatcc
     2521 atcctttcac tgtagggccg cgggaggaga gcgacggctc aggcttgccg cgtcccgccc
     2581 gccccgtccg tcccggcctg ccgggcggct tcttcgacca aggcgacggc ctgctggggc
     2641 gcatacccca ggcggcccat cttttcaaga aaagcgctca cctcgccccc ggcaaattcc
     2701 gccttgaccg cggcgatgcg ccccgtgtcc tgcgtgacgg tgcggccgct ggtgcgctgg
     2761 gtctgcacca ggccgccctg ctccagctcc tgcagggcct tttgcatcgt attggggttg
     2821 acggctgcct ccgccgccag ctcgcgcacc ggcggcaccc tgctgccggg cggcagcagg
     2881 ccggagatga tcgccagctg cagatgctcc accagctgga tatagatcgg ccggtccgat
     2941 ttgagatccc agttcatggc cgtcgtcctt tctttgtttg tactaatgga ttaatacaat
     3001 aatacaaaac ttttttaaaa aagtcaaggg ggaaagcaaa actaaaaaag agcagccgag
     3061 agtctgtttc tgtaagaaaa ccctgcccac gaggaggagt tgtgcgcgga gcacccttaa
     3121 atccaccctg cagctatcaa ggggagaggt ttcgccctgc gaaccgcctg cgcaagcggg
     3181 cagtttgggt ggattttctc agctttacat cagcttttcc ctcccggggg cgcggcagtt
     3241 tttaaccgcc ggttttgaca ctccgcaggg ctaaaaaggc cggcaatcca cagagattgc
     3301 cagccttttt gtactgtgtt tctttcaaga ccgttgagcc caaaccaatg ttttattctc
     3361 tcgaggcgcc taggccgccg ctcttttttg tgcaggtgtg cgctgcccgg cctggcaaaa
     3421 gggcgggggc agcgtctgga tgagccggcg cagggaacgc aggcccggga cggcggagaa
     3481 aaaagctgtc ttcgcccaca gaaacgcagg gcagagagca g
//
LOCUS       DBMDYA010000250         3516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6067_length_3516_cov_1.702489, whole genome shotgun sequence.
ACCESSION   DBMDYA010000250 DBMDYA010000000
VERSION     DBMDYA010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6067_length_3516_cov_1.702489"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="ACZPFK_07065"
     CDS             <1..442
                     /locus_tag="ACZPFK_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406106.1"
                     /translation="GRYLNAAVQMRTVLEENLEAAQYIECVNEHPLLSILDRTQEALN
                     YGGAENVLQSIEYYSRQIVEKYGRGNMLIRSYQVILDKVSDILKNLIWAKKYQRPSYR
                     ESAYKQFNSLITELQIPTENPDVEALRLEAVIEEGIAKARLFLG"
     gene            526..1092
                     /locus_tag="ACZPFK_07070"
     CDS             526..1092
                     /locus_tag="ACZPFK_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406107.1"
                     /translation="MKNSKKAARNWQTLLLFFALLSVPVCFMLVTFSRLSTVSLSLWL
                     LEISYIALAISLLLSAKGTRISSVILSFACGLLTAVRFVTSHKGIILEAIPRIFNDEY
                     FTIVYTFVGVLVFIVLALVFLYPQLGSLGAVMAAAYFLSNIIWRVDYFARGLREFAPA
                     MDISLLCRLFLAVALFFYFFDARKRPQK"
     gene            complement(1343..1738)
                     /locus_tag="ACZPFK_07075"
     CDS             complement(1343..1738)
                     /locus_tag="ACZPFK_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406108.1"
                     /translation="MLKLKFADNTEKEVLQDTVIYPSGSPSIRSRMEVHMSADAMPLQ
                     DFEVLMSDPAKTGTLRLIETGADEAGGEMIVRDTAYSAYTLVASVGKQRVDKLDYTSG
                     QAAGETHLVAVLEQLTYIEQQLAALGLRV"
     gene            complement(1758..2330)
                     /locus_tag="ACZPFK_07080"
     CDS             complement(1758..2330)
                     /locus_tag="ACZPFK_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406109.1"
                     /translation="MALYYNNTSIPQGNHVYINNQDCQKVIYNNVEVWRRSIYISNLI
                     SDSHEFSKSFSDIGYVKDSEWRYSTATISGHYYYFRFNVGCWGYSNGAGFGGWTKARH
                     DGATEIIGKPMSEGLDNGVYTGHILVQSSGQTWGLNVEYYRNNATQGRNWTKESMMVD
                     VTGLYQAGYTTPDAIYGKIGYFRGTKEFII"
     gene            complement(2342..3196)
                     /locus_tag="ACZPFK_07085"
     CDS             complement(2342..3196)
                     /locus_tag="ACZPFK_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406110.1"
                     /translation="MSTTYPQFPYSNFPEQIDNFPDAQDVSAELLPLVKQYKALFNSG
                     EYAACQKLLADHPQLKAAQSSALSANQVYDAIGALQTLFKTDIEQYLDGRVPQRIVLS
                     ETEPSGLAENDVWLQVVGSGAAATTVNFNIRNAEASFDTIHFDLTPEEIGAMKQQTYD
                     PENTGKVLYAVKADVLGDKGAESFATAAQGKTADELVSGAIPAAKAAQLATVRKIGSA
                     DFDGSADVTLAQMGAEPAFVKNDAFNKAFGTAAGTVCQGNDSRLSNARRASNISMSYN
                     GNLYITYS"
     gene            complement(3212..>3516)
                     /locus_tag="ACZPFK_07090"
     CDS             complement(3212..>3516)
                     /locus_tag="ACZPFK_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027782.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5048 domain-containing protein"
                     /protein_id="HIR1406111.1"
                     /translation="KNQVLQGGEYDHITSDALAMQRAEYENWKSTRLTDSVSIEALLI
                     PWLDVNKKVSYTPRYDPALGTLQYIVKHIEFSLKSGTMTVSMCRFFPYYPYIIDKL"
BASE COUNT          783 a          840 c          910 g          983 t
ORIGIN      
        1 cggcagatac ctgaacgcgg cggtacagat gcggacggta ttggaagaaa acctggaggc
       61 ggcacagtat attgaatgtg tgaatgaaca tcctctttta agcattttag atagaacaca
      121 ggaagcactc aactatggag gcgcagagaa tgttttacag agtattgaat actactctcg
      181 acagattgta gaaaaatatg gaagaggaaa tatgttgata aggtcctatc aggtcatatt
      241 agataaagtt tctgatattt tgaaaaattt gatttgggct aaaaagtatc agaggccttc
      301 ttatagagag tccgcatata agcagtttaa ttctcttatt actgagcttc aaattccaac
      361 tgagaatccg gacgtagagg ccttaaggct ggaagctgtt attgaagagg ggattgcaaa
      421 ggcaaggctg tttttgggct gagcttccaa agttgagata caaagctgag cgatacccaa
      481 tcggataata gcaaagataa atcaataaaa aggtggaatg aaagcttgaa aaattctaaa
      541 aaggcagcac ggaattggca gaccttgctt ttgttttttg cgctgttgtc ggtgccggtg
      601 tgttttatgc tggttacttt ttccaggctt agtacggtgt ccctttcttt atggctttta
      661 gaaatttctt atattgccct tgcgatatcc ttgctgctgt ccgcaaaagg gacacgtatc
      721 tccagcgtga tcttatcttt tgcctgcggc ctgttaacgg cggtcagatt tgtgacgtcg
      781 cataagggca tcatattaga agcgattccc cgcattttta atgacgaata ttttacgatt
      841 gtttatacat ttgtgggcgt gcttgttttt atcgtcttgg cgttggtgtt tttatatccg
      901 cagctcggta gcttgggggc ggtgatggcg gccgcttact ttttatccaa tattatatgg
      961 cgggtggatt actttgcgag aggattgaga gaatttgccc cggcgatgga catcagcctg
     1021 ttgtgccgcc tctttttggc tgttgccctg ttcttttatt ttttcgatgc gcgaaagcgc
     1081 ccccaaaagt gacataaccg ctggggggaa tcggacacgt tgttctgatc caaagcagaa
     1141 gctgtaaaaa aggcggatag agagattgtt ctctctatcc gcttttttgg tctttccgat
     1201 cgcaatggac taaaatttta tggcccgcat tcctttttga gagaagattc gcacttgctg
     1261 aaaaatgaag ttattttttc cggcagacga aagcggcacg acacctcctt tagagacctc
     1321 taaagaaatt cgggcttttt cctcacaccc gcagccccag cgccgccagc tgctgctcga
     1381 tataggtgag ctgttccagc accgccacca gatgggtctc gccggcagcc tggccgctgg
     1441 tgtaatccaa cttgtccacc cgctgtttgc ccaccgaggc gaccagagta taggcgctat
     1501 aggcggtgtc gcggacgatc atttcgccgc ccgcctcatc ggcacctgtc tcgatgaggc
     1561 gcagcgtccc ggttttggcc gggtcggaca tcaggacctc aaaatcctgc agcggcattg
     1621 cgtccgcgct catatgtacc tccatgcggc tgcgaatgga ggggctgccg ctggggtaaa
     1681 taacggtgtc ctgaagaacc tctttttcgg tgttgtctgc aaattttagt tttagcattt
     1741 ttattttcct ccctatttta tataataaat tcctttgttc cacgaaagta tcctatttta
     1801 ccgtaaatag cgtcgggagt cgtgtagcct gcttgatata gtcccgtgac atcgaccatc
     1861 atagactctt ttgtccagtt acgtccttga gtggcgttat tacgatagta ttctacgttt
     1921 agtccccagg tttgtccgga actttgtaca agtatgtgtc ccgtataaac accattatct
     1981 agaccttctg acataggttt accaataatt tctgtcgccc catcatgacg agctttagtc
     2041 caccctccaa agcctgcccc attggaatat ccccaacagc cgacattaaa tctaaaataa
     2101 taataatgac cactaatagt ggctgtagaa tatctccatt cggagtcttt tacataccca
     2161 atgtcggaaa atgatttact aaattcgtga ctatctgata ttaagtttga aatataaatg
     2221 gaacgtctcc acacctccac attgttataa atcactttct ggcagtcttg gttattgata
     2281 tatacatggt tgccttgcgg tatgctggta ttattgtaat acaacgccat tgcttctcct
     2341 cctacgaata ggtgatatag agattgccgt tgtagctcat cgaaatattg ctcgcccgtc
     2401 tcgcattgga caggcggctg tcgttgccct ggcagaccgt gccggccgcc gtgccgaacg
     2461 ccttgttgaa cgcgtcgttt ttgacaaagg ccggttcggc gcccatctgt gccagggtga
     2521 cgtcggcgct gccgtcaaag tcggcgctgc cgatcttgcg gaccgtagcc agctgagcgg
     2581 cttttgcggc cgggattgcg ccagagacaa gttcatccgc ggttttcccc tgggcggcgg
     2641 tggcaaagct ttccgccccc ttgtccccca aaacgtcggc tttgaccgcg tacaggacct
     2701 tgccggtgtt ttcggggtca taggtttgct gcttcatggc gccgatctct tccggcgtga
     2761 gatcgaagtg gatcgtgtcg aaggaggcct ccgcgttgcg gatgttgaag tttacggtgg
     2821 tggccgccgc cccgctgccg acgacctgca gccagacgtc attttccgcc agaccgctgg
     2881 gctctgtttc ggagaggacg atgcgctgcg gcacgcgccc gtcgagatac tgctcgatgt
     2941 ccgttttgaa cagggtctgc agggcgccga tggcgtcgta gacctggttg gcggacagcg
     3001 cgctggactg cgctgctttc aactgcgggt ggtccgccag cagcttttgg caagcggcgt
     3061 actccccgct gttaaacaaa gccttgtact gcttcaccag cggcagaagc tctgcgctga
     3121 cgtcctgcgc gtcggggaaa ttgtcgatct gttccggaaa gttggaatac gggaactgag
     3181 gataggttgt actcatttga tcaccctttc gttacaattt gtcgatgatg tagggatagt
     3241 aggggaagaa gcggcacatc gagacggtca tcgtcccgct tttcagcgaa aactcgatgt
     3301 gcttgacgat atattgcagc gtccccagcg cggggtcata gcgcggggtg taggagacct
     3361 ttttgttcac atccagccag gggatcagca gggcctcgat ggagaccgaa tccgtcaggc
     3421 gggtcgattt ccagttctca tattccgccc gctgcatcgc cagcgcatcc gaggtgatgt
     3481 gatcgtattc accgccctgt aagacctgat ttttgt
//
LOCUS       DBMDYA010000251         3503 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6094_length_3503_cov_1.454733, whole genome shotgun sequence.
ACCESSION   DBMDYA010000251 DBMDYA010000000
VERSION     DBMDYA010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_3503_cov_1.454733"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="tsaE"
                     /locus_tag="ACZPFK_07095"
     CDS             <1..507
                     /gene="tsaE"
                     /locus_tag="ACZPFK_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1406112.1"
                     /translation="NVVYFLKNIIPKIPPLRGGRGEKGEKCMGVYLSHCKEDTEALGA
                     RLAQELRGGGLVAFTGGLGAGKTAFCGGIARGLGCADPVSSPTFSIVNVYRGPVPFAH
                     FDMYRIHSEEDLYACSFYDYLDAGAAVAVEWSENIEDFIEEPCIRVAIENLGGDERKI
                     TVTGADSL"
     gene            504..1217
                     /gene="tsaB"
                     /locus_tag="ACZPFK_07100"
     CDS             504..1217
                     /gene="tsaB"
                     /locus_tag="ACZPFK_07100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1406113.1"
                     /translation="MITLALDSSGRAAGTAVLRDGELIYESYLNNGLTHSETLLPMVE
                     AALSATGIAIGDLGLLAVGAGPGSFTGLRIGMALAKGLALPTGLPCAGVSSLEALARG
                     VFLPDGAAVVAASDARRGEVYYAAFLREGEALRRLCPDTAGRPGPLEEVLAGCKKPVF
                     VVGDGAQVCYNSLREKLPLEPVPAHWRLGRAAGVALAGLALWQAGGAVPAGDLHPAYH
                     RLSQAQRERQQKLKESERS"
     gene            1214..1660
                     /gene="rpiB"
                     /locus_tag="ACZPFK_07105"
     CDS             1214..1660
                     /gene="rpiB"
                     /locus_tag="ACZPFK_07105"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1406114.1"
                     /translation="MSLIALGADHGGYALKEAVKAHLDELGVAYKDFGCFSEESVDYP
                     DMAAPPCEAVVSGECDKALLFCGTGVGISIAANKIKGIRACCCSDIFSAKYTRLHNDA
                     NALCLGGRVVGAGLAIELVDVFLNTAFQGGRHATRIAKIAALEEKR"
     gene            1755..2384
                     /gene="upp"
                     /locus_tag="ACZPFK_07110"
     CDS             1755..2384
                     /gene="upp"
                     /locus_tag="ACZPFK_07110"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1406115.1"
                     /translation="MKEPMILDHPLLQHKVSHLRDINTGTKEFKELVSEIAMLMCYEA
                     TRDLPTQEVEITTPITTDKFKVLAGRKLAVVPILRAGLGMVDGVLALIPAAKVGHIGL
                     YRDPASLEPVEYYCKLPADISEREVMVVDPMLATGGSASDAITQIKKRGAKSIKFLCI
                     IAAPEGIKRLHDAHPDVEIYAAALDKCLNEHGYIVPGLGDAGDRIFGTK"
     gene            2406..2642
                     /locus_tag="ACZPFK_07115"
     CDS             2406..2642
                     /locus_tag="ACZPFK_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406116.1"
                     /translation="MLKKLVVPLLAHLLLLAAAVWMLLVLLQQSVFTGVLKWVMVGCA
                     ALLILCIAAAYLLRVLDYLQQRKKAEKAQPPPQG"
     gene            complement(2998..>3503)
                     /locus_tag="ACZPFK_07120"
     CDS             complement(2998..>3503)
                     /locus_tag="ACZPFK_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR1406117.1"
                     /translation="SVMPTSKDETIAWHLWHIARIEDLVGNLLIADQSQVLNDAWLEK
                     LQINIRDTGNAMDDEQIIGFSKQVNKRELINYRNKVGCQTRSILKSLTKNELKRKPSA
                     EALARLVGEGGLLEVQESLWLLNFWGRHTVAGLVLLPLTRHHMMHLPDSFAIKQFRRE
                     GGTPFMS"
BASE COUNT          661 a         1115 c         1073 g          654 t
ORIGIN      
        1 aatgtggtat actttttgaa gaatatcatt ccaaaaatcc ctccgctgcg gggcggccgg
       61 ggagaaaaag gagaaaagtg tatgggcgtt tacctctccc attgcaagga ggacaccgag
      121 gcgctgggcg cgcggctggc acaggagctg cggggcggcg gcctggtggc gttcaccggc
      181 ggcctgggcg cgggcaagac cgcgttctgc ggcggcatcg cccgcgggct gggctgcgcc
      241 gacccggtga gcagccccac tttcagcatc gtcaacgtct accgcgggcc ggtgccgttc
      301 gcccacttcg atatgtaccg catccactcg gaggaggacc tgtacgcctg ctctttttac
      361 gattatctgg acgccggggc cgcggtggcg gtggagtgga gcgagaacat cgaggacttt
      421 atcgaggagc cctgcatccg cgtcgccatc gagaacctgg gcggcgatga gagaaaaatc
      481 actgtgacgg gagctgacag cctttgatca cccttgcgct cgattcctcc ggcagggcgg
      541 ccgggaccgc cgtgctgcgg gacggcgagc ttatctatga atcctatctc aacaacggcc
      601 tcacccacag cgaaacgctg ctgcccatgg tggaggcggc gctgtccgcc accggcatcg
      661 ccatcggcga tctgggcctg ctggccgtcg gggccggacc gggcagcttt accggcctgc
      721 gcatcggcat ggccctcgcc aaggggctgg ccctgccgac agggctcccc tgtgcgggcg
      781 tctcctcgct ggaggccctg gcccgggggg tctttctgcc cgacggcgcc gccgtggtcg
      841 ccgcgtccga cgcgcggcgg ggcgaggttt actacgccgc attcctccgc gagggagagg
      901 ccctgcgccg gctgtgcccc gacacggcgg gcaggcccgg ccccctggag gaagttctcg
      961 ccgggtgcaa aaaacctgtt tttgtagttg gtgacggggc gcaggtgtgc tataatagcc
     1021 tcagagaaaa actgccgctg gagcctgtcc ccgcccactg gcgtctcggc cgggccgccg
     1081 gggtcgccct ggcgggcctt gccctgtggc aagcgggcgg ggcggtcccc gccggcgacc
     1141 tgcaccccgc ctatcaccgg ctgagccagg cccagcggga gcggcagcaa aaattgaagg
     1201 agagtgaaag atcatgagtt tgatcgcact gggcgccgat cacggcggtt atgccctcaa
     1261 agaagccgtc aaggcacatc tggacgagtt gggcgtggcc tataaggact tcggctgttt
     1321 ttctgaggag agcgtggact atcccgacat ggccgcgccc ccctgcgagg ccgtggtctc
     1381 gggcgaatgc gacaaggcgc tgctgttctg cggcaccggc gtgggcatca gcatcgcggc
     1441 gaacaaaatc aagggcatcc gcgcctgctg ctgcagcgac atcttcagcg ccaaatacac
     1501 ccgcctgcac aacgacgcca acgcgctgtg cctgggcggc cgcgtggtcg gcgccgggct
     1561 tgccatcgag ctggtggacg tctttttgaa caccgctttc cagggcggcc gacacgccac
     1621 ccgcatcgcc aagatcgccg cgctggaaga aaagcggtaa agtttgtagt tcccgccctg
     1681 ccgcgcccgg cggcaatgac aggtgcggct gtccctttcc gtcccatcac tgccgccgga
     1741 gaatggagat acagatgaaa gagcctatga tcctggacca ccctctgctg cagcacaagg
     1801 tcagccatct gcgggacatc aacaccggca ccaaggagtt caaggagctg gtcagcgaga
     1861 tcgcgatgct gatgtgctat gaggccaccc gcgacctgcc cacccaggag gtggagatca
     1921 ccacccccat caccaccgat aagttcaagg tgctggccgg gcgcaagctg gctgtggtgc
     1981 ccatcctgcg cgcgggcctg ggcatggtgg acggcgtgct tgccctcatc cccgccgcaa
     2041 aggtcggcca catcggcctt taccgcgacc ccgccagcct ggagccggtc gagtactact
     2101 gcaagctgcc cgccgatatc tccgagcggg aggtcatggt ggtggacccg atgctggcca
     2161 ccggcggcag cgccagcgac gccatcaccc agatcaaaaa gcgcggcgcc aagagcatca
     2221 agtttttgtg catcatcgcc gcgcccgagg gcatcaagcg gctgcacgac gcccacccgg
     2281 acgtggagat ctatgccgcc gcgctggaca agtgcctcaa cgagcacggc tatatcgtgc
     2341 cgggcctggg cgacgcgggc gaccgcatct tcggcacaaa ataaacacag ggggagcgga
     2401 cccacatgtt gaaaaaactg gtcgtgccgc tgctcgccca tcttttgctg ctcgccgcag
     2461 cggtctggat gctgctggtg ctgctgcagc agagcgtttt taccggcgtg ctgaaatggg
     2521 tgatggtggg ctgcgctgcg ctgctgatcc tctgcatcgc cgcagcctac ctgctgcggg
     2581 tgctggatta cctgcagcag cgcaaaaaag cggagaaagc gcagcctccc ccgcagggct
     2641 gagccagagc cccgcctcca acgcgttgac gcgggaaagc atcggccctg gtccggcggc
     2701 ttttgagcct agccctttca aaaaggttgg caatccgtag ggattgccaa ccttttttac
     2761 gcaatttctt attcagggcc gtcaggccaa aattgccgcc ctttaccacg ggaaagttgc
     2821 ggggtaaaat ttgaaaaagg accgcccgcc gcctgcgacc cgcccgcggc attgaaaagg
     2881 attcaacgcg ttttatctct atcccctaaa cgcccggcgg gtcagcctgt aaacccgcaa
     2941 tttatcgagc gcgccctgtt taccggaggt acaaaccaca gccaaagcat ctttctctca
     3001 gctcataaaa ggcgtgcccc cctcacgtct gaattgcttt attgcgaagc tgtccggcaa
     3061 gtgcatcata tggtggcgcg tcaagggcaa cagtacgagc ccggccacgg tatgcctgcc
     3121 ccaaaaattc aaaagccaga gggactcctg cacctccagc aggccgccct cgccgaccaa
     3181 tcgtgccagg gcttccgcgc tgggcttccg cttcagctcg tttttggtca aggactttaa
     3241 gatgcttcgg gtctgacacc ccactttatt gcggtaattg atgagttccc gcttgtttac
     3301 ctgtttgctg aagccgatga tctgctcatc gtccatcgcg ttgccggtat ccctaatatt
     3361 gatctgcagt ttttccaacc acgcgtcatt caaaacctga gattgatccg ctatcagcaa
     3421 attacccacc aaatcttcaa tcctggctat atgccataaa tgccacgcta tggtctcatc
     3481 ttttgaggta ggcatcacac taa
//
LOCUS       DBMDYA010000252         3488 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6130_length_3488_cov_1.399469, whole genome shotgun sequence.
ACCESSION   DBMDYA010000252 DBMDYA010000000
VERSION     DBMDYA010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_3488_cov_1.399469"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="ACZPFK_07125"
     CDS             complement(<1..346)
                     /locus_tag="ACZPFK_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406118.1"
                     /translation="MPVFSFTFFGFFAAVFALYWLAPHRWRTALLFAASAGFYVLFGL
                     PYTLLLLACMAGCWAGGLWLGRGRSRLRLALCVALALAPLLLFKYLDFLAGGLAALSG
                     LLGAPFQAPYLKW"
     gene            698..2152
                     /gene="gltX"
                     /locus_tag="ACZPFK_07130"
     CDS             698..2152
                     /gene="gltX"
                     /locus_tag="ACZPFK_07130"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1406119.1"
                     /translation="MESNVRTRFAPSPTGYMHVGNLRTALYAYLKAKSCGGTFVLRIE
                     DTDQERYVEGAVDIIYNTLRETGLLWDEGPDKDGGYGPYVQSERMGLYLDYAKQLVQS
                     GHAYYCFCDKQRLDEMRTIQKASGQAPHYDGHCRNLSKEEIEENLAAGKPFVIRQKMP
                     REGVTGFDDVVYGHIEVENAELDDQILIKTDGMPTYNFANVVDDHLMAISHVIRGSEY
                     LSSTPKYNLLYEAFGWEKPVYIHCPPVMKDAQNKLSKRNGDASYQDLVAKGYLTEAIL
                     NYIALCGWSPKGENEIFTLPEMVKAFSEEGISRSPAIFDPEKLRFMNGEYIRRLSPEA
                     FYEKAQPWIDQAVRRACDKQLLAHVLQQRCEVLGDIPAQLPFIDEVQPHGLELYTSKK
                     MKTTPETAKAALEALIPALEALGEEDWTVEKIHAACFDLIAKMEVKNGWMLWPMRIAL
                     SGMQFTPGGGIEIAAILGREESLARLRAGLAELS"
     gene            2435..2980
                     /locus_tag="ACZPFK_07135"
     CDS             2435..2980
                     /locus_tag="ACZPFK_07135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1406120.1"
                     /translation="MAVQTFLTDYESPLGPLVLASDGERLTGLWMQDQKYFGSTLPEE
                     KALQWTEAPVFAQARRWLDLYFAGKKPDFTPPLAPAGSPFRQAVWAALRRIPYGGVTT
                     YGQIARELEQGDGRRVSAQAVGGAVGHNPISILIPCHRVVGADGSLTGYAGGVKKKLF
                     LLQLEGAPMEGLFAPKKGTAL"
     gene            complement(3235..>3488)
                     /locus_tag="ACZPFK_07140"
     CDS             complement(3235..>3488)
                     /locus_tag="ACZPFK_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406121.1"
                     /translation="RTQNVKKQALKTRRRVFFTLPHLKRGVCCEIFLSGEDRQTGKER
                     PLLLCHKRGFVSGPPFLAAAQQLKWRTAKWPAAIFIASQ"
BASE COUNT          723 a         1120 c         1062 g          583 t
ORIGIN      
        1 cccatttgag gtaaggggcc tggaacggcg cgcccagcag cccggacagc gcggcgaggc
       61 cgccggccag gaagtcaagg tatttgaaaa gcagcagcgg cgccagggcc agcgccacgc
      121 agagcgccag ccgcaggcgg gagcggcccc gtcccagcca gagccccccg gcccagcagc
      181 ccgccatgca ggccagcaac agcagcgtat agggcaggcc gaacagcacg taaaagcccg
      241 cgctggccgc aaacagcagc gccgtgcgcc agcggtgcgg cgccagccag taaagggcga
      301 acaccgcggc aaaaaaaccg aaaaaagtaa aagaaaaaac gggcaccggg ccgccccctt
      361 tccgtttttt gtcgataact ctgtttatta tacactgacc gaccgccccg cacaagaaaa
      421 gagacccgcc ggcgccctct tttcactttt gtgcgcccgg ccggtcctgc cgcccaccgc
      481 ctgcggcccg cgttttgcgc cgatgcgccc cgttttcagg ccgccgggac gggccttttg
      541 cgcggcggcg tttttctcgg cctttcccgc gtggaagaaa gccccaaaac ggcggctgca
      601 agcgcttttt cattgacaaa ccggtccgcg cggggtagac tttaagagta tgaccctgtg
      661 tcaaaagccc gataccttta tagaggagga agtatagatg gagagcaacg tgagaacgcg
      721 tttcgccccc agccccaccg gctacatgca tgtgggcaac ctgcgcaccg ccctgtacgc
      781 atacctgaaa gccaagagct gcggcggcac ctttgtgctg cgcatcgagg acaccgacca
      841 ggagcggtat gtggagggcg cggtggacat catctacaac accctgcgcg agaccggcct
      901 gctctgggac gagggcccgg acaaggacgg cggctacggc ccctatgtgc agagcgagcg
      961 catgggcctg tatctggact acgccaagca gctggtgcag agcgggcacg cctattactg
     1021 cttctgcgac aagcagcggc tggacgagat gcgcaccatc cagaaagcca gcggccaggc
     1081 cccccactac gacggccact gccgcaacct ctcaaaagag gagatcgaag agaacctcgc
     1141 cgccggaaag cctttcgtca tccgtcagaa aatgccgcgg gagggcgtga ccggctttga
     1201 cgacgtggtg tacggccaca tcgaggtgga aaacgccgag ctggacgatc agattctcat
     1261 caagaccgac ggcatgccca cctataattt cgccaacgtg gtggacgacc atttgatggc
     1321 catcagccat gtcatccgcg gcagcgaata cctctcctcc acccccaagt acaatctgct
     1381 ctacgaggcg ttcgggtggg agaagccggt gtatatccac tgcccgccgg tgatgaagga
     1441 cgcgcagaac aagctgtcca agcgcaacgg cgacgccagc taccaggacc tggtggccaa
     1501 gggctatctc accgaggcca tcctcaacta tatcgccctg tgcggctgga gccccaaggg
     1561 cgaaaatgag atcttcaccc tgcccgagat ggtaaaggcg ttcagcgagg agggcatcag
     1621 ccgctcgccg gccatcttcg acccggaaaa gctgcgcttc atgaacgggg agtatatccg
     1681 ccgcctgtcg cccgaggcgt tctatgaaaa agcccagccc tggatcgacc aggcggtgcg
     1741 ccgcgcctgc gacaagcagc tgctggccca cgtgctgcag cagcgctgcg aggtgctggg
     1801 cgacatcccc gcccagctgc cctttatcga cgaggtgcag ccccatgggt tggagctcta
     1861 caccagcaaa aagatgaaga ccacccccga gaccgccaag gcggcgctgg aggccctgat
     1921 cccggcgctg gaggccctgg gcgaggagga ttggacggtg gaaaagatcc acgccgcctg
     1981 ctttgacctg atcgccaaaa tggaggtcaa gaacggctgg atgctctggc cgatgcgcat
     2041 cgcgctgtcc ggcatgcagt tcaccccggg cggcggcatc gagatcgccg ccatcctggg
     2101 ccgcgaggag tctcttgccc gcctgcgggc cggcctggcg gagctgtctt aaaaccgggc
     2161 ggcgggcccg gcccccagcg acccgcccgg cggacaaaag cacgcgccct aaaggacgcc
     2221 gaaacgggca gcctgtcccc ccacggacgg ctgcccgttt ttttgaatca tgagccgctg
     2281 cctgggagaa gcctttctcc gccgccggac cctgcccgga aagatgcggc ccggccggca
     2341 gcaaaaaggc ttccggcggc ggacggcggc gaacgaaccc tgcgccgcag cctctgggga
     2401 acgcccggaa cttgaaaaaa ggagcggatg cgacatggcg gtacagacct ttttgacgga
     2461 ctatgaatcc cccctcggcc cgctggtgct ggcctccgac ggcgagcgcc tcaccgggct
     2521 gtggatgcag gatcaaaaat atttcggcag cactttgccc gaggagaaag cgctgcagtg
     2581 gacggaggcg cccgtctttg cacaggcacg ccgctggctc gacctttatt ttgcgggcaa
     2641 aaagccggac ttcaccccgc ccctcgcccc ggcgggcagc ccgttccgcc aggcggtgtg
     2701 ggctgccctg cgccgcatcc cctacggcgg cgtcaccacc tacgggcaga tcgcccgcga
     2761 gctggaacag ggcgacgggc gccgcgtctc cgcccaggcg gtgggcggtg cggtggggca
     2821 caaccccatc tcgattttga tcccctgcca tcgggtggtg ggcgccgacg gcagcctgac
     2881 cggctacgcg ggcggggtga aaaaaaagct tttcctcctc caattggagg gcgcgcccat
     2941 ggagggcctg ttcgccccca aaaaggggac ggcactctaa aaatagccgt gctacggcta
     3001 tttttcggtc ctgcggccag tccctctggc cggagccatt ggaataagtt gtttttattg
     3061 cgaagcaata aaaatagccg cggcgcggct atttttcggc catccggcca gtcctctggc
     3121 cggggccatt gaaataagtt gtttttattg cgaagcaata aaaatagccg cagcgcggct
     3181 atttttcggc catccggcca gtcctctggc cggggccatt ggaataagtt gtttttattg
     3241 cgaagcaata aaaatagccg caggccattt tgcagttctc catttcagtt gctgcgcagc
     3301 ggcgagaaac gggggtcctg aaacaaatcc tcttttatgg caaagcagca aaggcctctc
     3361 tttcccggtc tgccggtcct ccccagacaa aaatatctca cagcaaacgc cgcgcttcaa
     3421 gtgtgggagg gtaaaaaata ctcttcgccg cgtctttaag gcctgttttt ttacattttg
     3481 cgtcctcc
//
LOCUS       DBMDYA010000253         3472 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6168_length_3472_cov_1.471077, whole genome shotgun sequence.
ACCESSION   DBMDYA010000253 DBMDYA010000000
VERSION     DBMDYA010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6168_length_3472_cov_1.471077"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="ACZPFK_07145"
     CDS             complement(<1..747)
                     /locus_tag="ACZPFK_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR1406122.1"
                     /translation="MGFTVEAGIPAVTVFLQGILSFFSPCVLPLVPLYIGYLAGGAKT
                     VAADGSIRYGRKKVLLNTLFFVLGVSFAFFALGLGFTAAGRFFSGHRTLFARVGGILV
                     MLFGLYQLGLFGGSGALSREHRLPFRLDRFAMNPLVALVLGFTFSFAWTPCVGPALAS
                     VLLMASSAQSTALGFVLIGVYTLGFVLPFLAVGLFTGTLLDFFRKHQKVVRYTVKAGG
                     ALLVLMGVMMFTGWMNGVTGYLSGLGGGAAP"
     gene            complement(850..2220)
                     /locus_tag="ACZPFK_07150"
     CDS             complement(850..2220)
                     /locus_tag="ACZPFK_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1406123.1"
                     /translation="MKKFDAVIIGFGKGGKTLAGALAAAGKTVALAERSDKMYGGTCI
                     NVACIPTKALNHSAALSAAMGGDFDEKAARYRAAIAEKRALTAKLRQKNYDKLASNPK
                     ITVLQGAARFTGPHTVSVSGPQGIQELWGENLFINTGARPFVPPIPGLAGNPRVYLSE
                     TLMELEQLPRRLVIIGGGYIGMEFASIWANFGSQVTVVQDGETFLPREDAEIAAAVLE
                     SLNARGVAVLRGAKIEAVREGEGTALVDLTMGGEARTLEAEAVLVATGRRPELEGLHP
                     EAAGVELTPRGAVKVDAQLRTTAPHIFAMGDVAGGLQFTYISLDDSRIVRSALLGDGG
                     RTTENRGAVPYSVFLDPPFSRVGLTEQEARDAGYQVKIARLPAAAIPKAQILRQPTGL
                     LKAVVDADSGRVLGAHLFCAESHEMINLLKLAVDQKIPYTALRDAVYTHPTMSEALND
                     LFSAVG"
     gene            complement(2235..2663)
                     /locus_tag="ACZPFK_07155"
     CDS             complement(2235..2663)
                     /locus_tag="ACZPFK_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1406124.1"
                     /translation="MTSEFSVAVHALVFLNHKGCCVPSELLADNICTNPARVRKIMAQ
                     LKKAGLVETREGAEGGYRFTGDPAATTLSQIGEALDARYVCANWHSGDVDKECLISSG
                     MGAILDGIYDDLNEVCRAHLAAITIDAIDKKIFAHTAEQP"
     gene            2958..>3472
                     /locus_tag="ACZPFK_07160"
     CDS             2958..>3472
                     /locus_tag="ACZPFK_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1406125.1"
                     /translation="MRIEPFKVEEWMNAYETQAVYNIAETCVESISLEELLELCGEQR
                     SPFLDALAARRLTYGDIVGAPAYLKGICGLYRTIGPGQVVSTHGAAGANHLLLYSLVE
                     PGDRVISVLPTYQQLYSIPASFGAEVRLLRLRRENGYLPDPDELRRLAAGGAKVICIN
                     NPNNPTGALMP"
BASE COUNT          633 a         1131 c         1147 g          561 t
ORIGIN      
        1 gggcgccgcc ccgccgccca gcccggacag atagccggtg acgccgttca tccagccggt
       61 gaacatcatc acgcccatca gcaccagcag cgcgcccccg gccttgacgg tgtagcgcac
      121 caccttttgg tgttttctga aaaagtcgag cagcgtgccg gtgaacagcc ccaccgccag
      181 aaagggcagc acaaagccca gcgtgtacac gccgatgagc acaaagccca gcgcggtgct
      241 ctgcgccgag gaggccatca gcagcacgct tgccagagcg ggccccacgc agggggtcca
      301 ggcaaagctg aaagtgaatc ccagcaccag cgccaccagc gggttcatgg cgaagcggtc
      361 cagccggaaa ggcagcctgt gctcgcggga gagggcgccc gagccgccga acaggcccag
      421 ctggtagagg ccgaacagca tcaccaggat gccgcccacc cgggcaaaca aggtgcggtg
      481 gccgctgaaa aaccgcccgg cggcggtgaa gcccagcccc agcgcgaaaa aggcaaagct
      541 caccccgagc acaaaaaaca gggtgttgag caacaccttt ttgcgcccgt agcggatgga
      601 gccgtccgcg gcgacggtct tggcgccgcc cgccagatag ccgatataca acggcaccag
      661 cggcagcaca cagggcgaga aaaagctcag gatgccctgc agaaaaacgg tgacggccgg
      721 gatgccggcc tcgacggtaa agcccataag aacctcctaa gggggatggt agtcagcgaa
      781 gatcaggcgg ccgggcccgc acagccgcgc ggggcggatg cgggggcggc gccctttgcg
      841 gcagggcgct cagcccaccg cagagaagag gtcgttcagc gcctcggaca tggtgggatg
      901 ggtgtagacg gcgtcccgca gcgccgtgta ggggattttc tgatccacgg ccagcttgag
      961 cagattaatc atctcatgag actcggcgca gaacaggtgg gcgcccagca cgcggccgct
     1021 gtcggcgtcg acgacggctt tcaagagccc tgtgggctgc cgcaggatct gggctttggg
     1081 gatggcggcg gccggcagcc gcgcgatttt cacctggtaa cccgcgtccc gggcctcctg
     1141 ctcggtcagg cccacgcggg agaaaggcgg gtcaagaaag acgctgtagg gcaccgcgcc
     1201 ccggttttcg gtggtgcggc cgccgtcccc cagcaaagcc gagcgcacga tgcgcgagtc
     1261 atccagcgag atataggtga actgcagccc gccggccaca tcccccatgg cgaagatgtg
     1321 gggcgcggtg gtgcgcagtt gcgcgtccac tttcaccgcg ccgcgcgggg tcagctccac
     1381 cccggcggcc tccgggtgca ggccctccag ctccgggcgg cggccggtgg ccaccagcac
     1441 ggcctcggcc tccagcgtcc gggcctcgcc gcccatcgtc aggtcgacca gggcggttcc
     1501 ctcgccctcg cgcacggcct cgatctttgc gccgcgcagc accgccacgc cgcgcgcgtt
     1561 caggctctcc agcacggcgg cggcgatctc tgcatcctcc cgcggcaaaa aggtctcacc
     1621 gtcctgcacg acggtcacct gcgagccgaa gttggcccag atggaggcga actccatgcc
     1681 gatatagccg ccgccgatga tgaccagccg ccggggcagc tgctccagct ccatcagggt
     1741 ctcgctgaga tagacgcggg ggttgcccgc aaggccgggg atgggcggca caaagggccg
     1801 cgcgccggtg ttgataaaga ggttttcgcc ccacagctcc tggatgccct gcgggccgga
     1861 caccgacacg gtgtgggggc cggtgaagcg cgcggccccc tgcagcacgg tgatctttgg
     1921 gttggaggcc agcttgtcgt aatttttttg ccgcagcttg gcggtgaggg cacgtttttc
     1981 tgcgatcgca gcccggtagc gggcggcctt ttcgtcaaaa tcgccgccca tggccgcgga
     2041 cagggccgcg ctgtggttga gggccttggt ggggatgcag gccacgttga tgcaggtgcc
     2101 gccgtacatt ttatccgagc gctcggccag cgccaccgtc ttgccggccg ccgccagcgc
     2161 ccccgccagc gttttgccgc ccttgccgaa accgatgatg accgcgtcga attttttcat
     2221 gtgattgcct cttttcaggg ttgctccgcc gtgtgggcga agatcttttt gtcgatggcg
     2281 tcgatggtga ttgcggccag atgcgcgcgg cagacctcgt tcagatcgtc gtagatgccg
     2341 tccaaaatcg cccccatgcc ggaggagatc agacattcct tgtccacgtc cccgctgtgc
     2401 cagtttgcgc agacatagcg ggcgtccagc gcctcgccga tctgggacag agtcgttgcg
     2461 gcggggtctc cggtgaagcg gtagccgccc tccgccccct cgcgggtctc caccaggccc
     2521 gcctttttca gctgggccat gatcttgcgc acccgcgccg ggttggtgca gatgttgtcc
     2581 gccagcagct cgctgggcac acagcatccc ttgtggttga gaaagacgag cgcgtggacc
     2641 gccacgctga attcactggt catgagaggg tccctccaaa actgtaactg aaataagctc
     2701 atttctaact gtaattatac caattacaat ttgaatgtca agagattttg cccgcttttg
     2761 caaaattttt cggcacaggg cgcggggagc ggcaaaaatt tgcgccgcgc cctccccgcc
     2821 ggcgggcaag tgtggtaaaa tatagtctat ctatacaaga tggacaaagc ggcgcccccc
     2881 tgcggcgcgg ctgaggggcg agggccgcac ccggcgcagg gccccgggac aaacggaaag
     2941 acaaaggagc tgtgagcatg aggatcgaac cctttaaagt ggaagagtgg atgaacgctt
     3001 acgagacgca ggcggtttac aacatcgccg agacctgcgt ggagtcgatc tcgctggagg
     3061 aattgctgga gctgtgcggc gagcagagaa gcccgttttt ggacgcgctg gcggcccgcc
     3121 ggctgaccta cggcgatatt gtgggcgcgc cggcctacct gaagggcatc tgcgggctct
     3181 accgcaccat cgggccgggg caggtggtct ccacccacgg cgcggcggga gccaaccatc
     3241 tgctgctcta ctccctggtg gagccgggcg accgggtgat ctcggtgctg cccacctatc
     3301 agcagctcta ctcgatcccc gcgtcctttg gggccgaggt ccggctgttg cggctgcgcc
     3361 gtgaaaacgg ctatctgccc gacccggacg agctgcgccg cctggcggcc ggcggcgcca
     3421 aggtgatctg catcaacaac cccaacaacc ccaccggcgc gctgatgccg aa
//
LOCUS       DBMDYA010000254         3469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6170_length_3469_cov_1.607779, whole genome shotgun sequence.
ACCESSION   DBMDYA010000254 DBMDYA010000000
VERSION     DBMDYA010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6170_length_3469_cov_1.607779"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            338..1354
                     /gene="add"
                     /locus_tag="ACZPFK_07165"
     CDS             338..1354
                     /gene="add"
                     /locus_tag="ACZPFK_07165"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR1406126.1"
                     /translation="MSHYDLPKIDLHLHLDGSVLPETAWELARQQGVPLPADTLEEFR
                     HFIVVTADCRSVNEYLKRFELPLQVLQDKASLARVTRELIGLLASQGLAYAEIRFAPQ
                     LHLRGGLTQRDAVEAVLAGRAEGLAANPGIGAGIILCAMSVGPESANMAENRETVRLA
                     AEYLGRGVAAVDLAGAEGIVPLKNFAPVFDLAKELGVPFTCHAGDSQGPDTVRDALDF
                     GARRIGHGHHIFEDKALCERAVRDGVTLEICPTSNVQCQSRPGYAEHPAKQLFDMGMR
                     ITINTDNMILSGIDLDTEYDHCLNEMGFCRADLLQMLVYSAEAAFLPQPEKEKLLARI
                     RACA"
     gene            complement(1664..1924)
                     /locus_tag="ACZPFK_07170"
     CDS             complement(1664..1924)
                     /locus_tag="ACZPFK_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406127.1"
                     /translation="MKGNGCRSGWRYLIPALGVALATISFFLFLVNESYIEPWLYMMF
                     IITGGGGLFFLLPSVALAPRSDALRDAFCCCGSMAASVAQAP"
     gene            2154..2861
                     /locus_tag="ACZPFK_07175"
     CDS             2154..2861
                     /locus_tag="ACZPFK_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1406128.1"
                     /translation="MKECVLVVDDDREIVGAIALLLEDEGYRVLRAYDGLEALDLVAR
                     ESVQLILMDVMMPRLDGLSAVLRLREQRNLPIIVLSAKSEDTDKVLGLSMGADDYIAK
                     PYNPLELVARVRSQLRRYTRLGGIDTLSADEIVNGRLRYNKAERVLRADGEPVRLTAT
                     ETGIVDLLMRNLGRIFPAEEIYRRVWQEDAFAPENTVMVHIRRIREKIELNPKEPEYL
                     KVVWGIGYKIEKVESHS"
     gene            2824..>3469
                     /locus_tag="ACZPFK_07180"
     CDS             2824..>3469
                     /locus_tag="ACZPFK_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406129.1"
                     /translation="MDTKSKKWKVILSFVCFFLGVSLLLCAIGPAVQLARRGNEAWGV
                     RDVFADDYQNTQAFRNQISNRLEQFLTMAAGGPVDGWYGWYDGYDGYDGVTISQGLGY
                     GGEGWNWGSAAAEGTAEALSEEEKEEYAQARKEWAQRARELAVELANDKNLLYTVSRD
                     GSVKYTNAEGLPLDGAAGKLPEGYNFLLYFDGEKVTVQKDGQTLDVYGDGYYDEE"
BASE COUNT          628 a         1098 c         1191 g          552 t
ORIGIN      
        1 tcggaacaaa gctttttgcg ccgcccgggc ggcaagagcg ccgcggcggc ccccggggcg
       61 acggttttcc aggcgtcccc ctgcgcggcg tcccatacgg cggttccccg tggggcggcg
      121 tctttcggcg ctctttgagt acccgcattg cgcccgcccc gccagacagt ctttcccgcg
      181 cggcggcgtc tttcggtcgc gccatcggcg cggggcgcgg gccccgcgtt tcgcccaccg
      241 gccgccagcc ttgcggccgc tcgtccgcgg tcacgcgtcc cgcagcggcg ccgctttttc
      301 atgcaaccaa acttttcctt ttgaggaggg accgaacatg tctcattacg acctgcccaa
      361 gatcgacctg cacctgcatc tggatggctc cgtgctgccg gagacggcct gggagctggc
      421 gcggcagcag ggtgtgccgc tgcccgccga cacgctggag gagttccgcc atttcatcgt
      481 cgtgaccgcg gactgccgca gcgtcaacga atacctcaag cgctttgagc tgccgctgca
      541 ggtattgcag gacaaagcct cgctggcccg ggtcacccgg gagctgatcg gcctgctggc
      601 ctcccagggg ctggcctatg ccgagatccg ctttgcgccc cagctgcacc tgcgcggcgg
      661 cctgacccag cgggacgcgg tggaggcggt gctggcgggc cgggccgagg ggctggccgc
      721 caacccgggc atcggcgcgg gcattatcct gtgcgccatg agcgtcgggc ccgagagcgc
      781 caacatggct gaaaaccgtg agacggtgcg gctggcggca gaatatctgg gccggggcgt
      841 cgcggccgtg gacctggcgg gcgcggaggg catcgtgccg ctgaaaaatt ttgccccggt
      901 ctttgacctg gccaaggagc tgggcgtgcc gttcacctgc cacgccgggg acagtcaggg
      961 gccggacacg gtgcgggacg cgctggactt cggcgcccgc cgcatcgggc acggccacca
     1021 catttttgag gacaaggccc tctgcgagag ggcggtgcgc gacggggtga cgctggagat
     1081 ctgccccacc agcaacgtgc agtgccagag ccgccccggc tatgccgagc acccggccaa
     1141 acagctgttc gacatgggga tgcggatcac catcaacacc gacaacatga tcctgtccgg
     1201 catcgacctg gacacggaat acgaccactg cctgaacgag atgggctttt gccgcgccga
     1261 cctgctgcag atgctggtct attccgccga ggccgccttt ctgccgcagc cggaaaagga
     1321 aaagcttctg gcccgcatcc gggcctgtgc gtgaatttta tttgaaaaag ggactcggcc
     1381 ccggcgggcc tgagtccctt ttgttcggcc ggggtcagcc ggcgctgtgg ccgtcccggc
     1441 cgccgggcgg gtagggttcg gccgctgtag tcataagcct gataggcggg cctgccgccg
     1501 cagtccgggg cctcgcgtcg gctgccgcag ccggcatcgg cgcgcaggaa gcgccagatg
     1561 ccgccgcaca gcagcgtgag gaagaaaaag cagaacgcca ccccgaagac cgacagcgga
     1621 ttgcagctgc agcagtcgtt cagcgagagg aagaacgccg tgatcagggc gcctgcgcca
     1681 ccgaggccgc catgctgccg cagcagcaga acgcgtcgcg cagcgcgtcg ctgcggggcg
     1741 ccagcgccac cgacggcagc agaaagaaaa ggccgccccc gcccgtgatg atgaacatca
     1801 tgtagagcca gggctcgata taggactcgt tcaccagaaa aagaaaaaag gagatcgtgg
     1861 caagggcgac gcccaacgcc gggatcaggt aacgccagcc gcttctgcag ccgtttcctt
     1921 tcaaaaagat cactcctttg ctgtgatggg ggcccgccct gcgccccttt ccgcacgggc
     1981 ggaccgttcc cgcccccagt gtaggagcag ctgccggctt tattctcgtc gtgccgcccc
     2041 ggcaaaccgt aagacttccg taagaaatcc gtcggccgtt ttgtaagaac cggcccctac
     2101 aataaagaca ggctcaaggg ggacgcggcc gctcccccgg aaagggaatg cagatgaaag
     2161 aatgtgtgct ggtggtcgac gacgaccgcg agatcgtggg ggcgatcgcc ctgctgctgg
     2221 aggacgaggg ctaccgggtg ctgcgggcct acgacgggct ggaggcgctg gacctggtgg
     2281 cgcgggaatc ggtgcagctg atcctgatgg atgtgatgat gcccaggctg gacggccttt
     2341 ccgccgtgct gcggctgcgc gagcagcgca acctgcccat catcgtgctc agcgccaaaa
     2401 gcgaggacac cgacaaggtg ctggggctct cgatgggggc ggacgactat atcgccaagc
     2461 cctataaccc gctggagctg gtggcccggg tgagaagcca gctgcgccgg tacacgcggc
     2521 tggggggcat cgacaccctt tcggcggacg agatcgtcaa cgggcggctg cggtacaaca
     2581 aggccgagcg ggtgctgcgc gccgacggcg agccggtgcg gctcaccgcc accgagaccg
     2641 gcatcgtcga cctgctgatg cgcaacctgg ggcggatctt ccccgccgag gagatctatc
     2701 gccgcgtctg gcaggaggac gctttcgcgc ccgaaaacac cgtgatggta cacattcgcc
     2761 gcatccgtga aaagatcgag ctgaacccga aagagccaga atatttaaag gtggtatggg
     2821 gcattggata caaaatcgaa aaagtggaaa gtcattctta gcttcgtctg tttctttctc
     2881 ggcgtctcgc tgctgctgtg cgccatcggg ccggcggtgc agctggcccg ccggggcaat
     2941 gaggcctggg gggtacggga cgtttttgcc gacgattacc aaaacacaca ggcctttcga
     3001 aaccagatct ccaaccggct ggagcagttt ctcaccatgg ccgcgggcgg cccggtggac
     3061 ggctggtacg gctggtacga cggctatgac ggctacgacg gcgtcaccat ctcgcagggg
     3121 ctgggctacg gcggcgaggg ctggaactgg ggctccgccg cggccgaggg cacggcggag
     3181 gccctgtctg aggaagaaaa agaagaatat gcacaggccc gcaaggagtg ggcccagcgc
     3241 gcccgcgagc tggccgtcga gctggcgaac gacaagaacc tgctctacac ggtcagccgg
     3301 gacggcagtg tgaaatacac caacgccgag ggcttgccgc tggacggcgc ggcgggtaag
     3361 ctgccggagg ggtataactt tttgctctac tttgacggcg aaaaggtcac cgtgcaaaag
     3421 gacggccaga cgctggacgt ctacggcgac ggctattacg acgaggagg
//
LOCUS       DBMDYA010000255         3467 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6174_length_3467_cov_1.597742, whole genome shotgun sequence.
ACCESSION   DBMDYA010000255 DBMDYA010000000
VERSION     DBMDYA010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_3467_cov_1.597742"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="ACZPFK_07185"
     CDS             <1..222
                     /locus_tag="ACZPFK_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406130.1"
                     /translation="NKKQPQRSGGYFFGHVAHPWATAIKISCFLLRSNKKQLSSHLWD
                     KSCCFCGTTRIGAASLRRPLARAQKCAPL"
     gene            complement(638..714)
                     /locus_tag="ACZPFK_07190"
     tRNA            complement(638..714)
                     /locus_tag="ACZPFK_07190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Ile,seq:cat)
     gene            908..2683
                     /locus_tag="ACZPFK_07195"
     CDS             908..2683
                     /locus_tag="ACZPFK_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1406131.1"
                     /translation="MEMNEESTAKKDLRGVPAALRSGLGKIFGPVGRWVGRHKKTAVA
                     LVLLAVAGGLFSWRFLAAGQSAGGGAAPSFIRTTTLQKTSLENSISATGTVGSAEVSN
                     VTTQLKYTVKEVNVQVGDTVQKGDVICTLDPSEIEKQIQKAKEALTENTEKAQASYDR
                     ALNSYNSAYDKASAAETAYNNAAAAYNSASSAFESAKAAVAALQSAYDAAAASAQAAA
                     ADVAAKLDAYTAAVTAAGGDLSATAVTDAARALNDACSAYNGTSFTAGGTVDTASQGG
                     GLAAAAAAALQALNSGKELCNYTALEQALNAAASAKEQARTQLDTLESQVASAKEQLS
                     TAESTLKDSKTSDTLEDLQDQLEECTLKAASSGTVTALNATVGSAVSDSTVATIQDTG
                     KLKISFSVEEYDISNVKVGLPVNITSDATGDTVIHGTVTQVSKTASSGQSGSGGFACE
                     ATVDDRDTGLLIGMNAKLSIILSTTDNVFVVPIDAVGTDENGGSVVYVKTGGSGVQAA
                     FEAVSVTTGEENDYYIEISGDGLEEGMEVRSSANAEEAEVGGGSSGADGAAGGMGGFA
                     VMGGAMAMPDGGGAPGGGGGGPMGG"
     gene            2687..3442
                     /locus_tag="ACZPFK_07200"
     CDS             2687..3442
                     /locus_tag="ACZPFK_07200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406132.1"
                     /translation="MTQDIFETRAGGAAPGEPLIAIRDLVKTYYIGKPNELEILHGIS
                     LTVWPGEFVAIVGESGSGKSTMMNIIGALDRPTSGTYHLDGVDVEKATDPQLSAIRNR
                     KIGFVFQTFNLIGRTTAQKNVELPMLYAGLPARQRSARAKALLEMVGMADRARHQPSE
                     LSGGQKQRVAIARAMACDPALLLADEPTGALDSQTSRTVMDIFHDLHEQQGKTIVLIT
                     HNPELAEECGRILTLKDGRIISERKGSGARGTA"
BASE COUNT          667 a         1163 c         1036 g          601 t
ORIGIN      
        1 aataaaaaac agccgcagcg aagcggaggc tatttttttg gccatgtggc tcatccttgg
       61 gccacggcca ttaaaatcag ttgtttttta ttgcgcagca ataaaaaaca gctttcatcc
      121 catctctggg acaaaagctg ttgcttctgc ggtaccaccc ggattggcgc cgcctctctg
      181 cggcgccctc tcgcacgcgc acaaaaatgc gcgccccttt gataacggac ggggtccccg
      241 gcgggcgcta ctgggcgatt gccgttcgcg ctgccctcgc aaggccattc ggcacggcgc
      301 tcaccgctgc ggttccaccc tccgcggctc tctggcaggc ttgctctccg tgcgtacttc
      361 ctcttgctca tcggttttgc tcttttgttt tgcttattat atgcgtttct ccccgctttg
      421 tcaagccgcg tccgcagctg cgagagggat gcgcaccaac gagtgtcttc ttcacaaatc
      481 gcgctccggg tgcgctcctc cgctctttct ccccagcgcg cctctgctag accggctctg
      541 cctttctcgg tctgctttct tctccgcttt ctttcgaggc cttgtcgata ccgccaaacg
      601 aaagataccc gccatttggt ggcgggtatc tttcgtttgg tgggccttca gggattcgaa
      661 cccgggaccg atcggttatg agccgagtgc tctgaccagc tgagctaaag gcccgcgcgc
      721 tcgtcgagac gcttatttat tatacacgaa tttttctttt cctgcaactg ggccctcctg
      781 ccgccctatc gcaaattcca cacattcctt ttggattttt cacacacttc gtcccgcgtt
      841 ttcacacatt cccggcctag aatgaagccc ggacagccac aaaaccattt tttacaagaa
      901 gaggagcatg gaaatgaacg aagagagcac cgctaaaaaa gatctgcgcg gggtcccggc
      961 cgccctgcgc agcggccttg gcaaaatttt cgggcccgtg ggccgctggg tggggcggca
     1021 caaaaagacc gctgtcgccc tggtcctgct ggccgtcgcc ggcggccttt tcagctggcg
     1081 ctttctcgcc gccggacaga gcgctggcgg cggcgcggcc ccctctttca tccgcaccac
     1141 caccctgcag aaaaccagcc tcgaaaattc catctccgcc accggcacgg tgggcagcgc
     1201 cgaggtcagc aatgtgacca cccagctcaa atacacggtc aaggaggtca acgtccaggt
     1261 gggcgacacc gtgcagaagg gggacgtcat ctgcacgctt gacccctctg agatcgaaaa
     1321 gcagatccaa aaggccaaag aggcgctcac ggaaaacacc gaaaaggcac aggccagcta
     1381 cgacagggct ctcaacagct ataacagcgc ctatgacaag gccagcgcgg ccgaaaccgc
     1441 ctacaacaat gccgcagccg cctacaacag cgcctccagc gcttttgaga gcgccaaggc
     1501 cgccgtcgct gcgctccagt ccgcctatga cgcggcggct gccagcgccc aggcggccgc
     1561 cgccgacgtg gccgccaagc tggacgccta caccgcggcg gtgacggccg cggggggcga
     1621 cctctccgcg accgctgtca ccgatgccgc gcgcgccttg aacgacgcct gcagcgccta
     1681 taacggcacc tctttcactg cgggcggcac ggtggacacc gcttcccagg gaggcggcct
     1741 ggccgctgcc gcggcggcgg cactgcaagc gctcaacagc ggcaaggagc tgtgcaacta
     1801 caccgctttg gagcaggctc tcaacgccgc cgcctccgcc aaagagcagg cccgcaccca
     1861 gctggacacc ctggagagcc aggtcgccag cgccaaggag cagctgtcca ccgctgaaag
     1921 caccctcaag gacagcaaga ccagcgacac gctggaggac ctgcaggacc agctggagga
     1981 atgcactctt aaggccgcct cctcgggcac cgtcaccgcc ctcaacgcca cggtgggcag
     2041 cgcggtctcc gacagcacgg tcgccaccat ccaggacacc ggcaagctga agatctcttt
     2101 ttcagtggag gagtacgata tctccaacgt caaggtgggc ctgccggtga acatcaccag
     2161 cgacgccacc ggcgacacgg tcatccacgg caccgtcacc caggtctcca aaaccgcctc
     2221 cagcgggcaa agcggctcgg gcggctttgc ctgtgaggcc acggtggacg accgggacac
     2281 cgggcttctc atcggcatga acgccaagct gagcatcatc ctctccacca ccgacaacgt
     2341 ttttgtggtg cccatcgatg cggtgggcac tgacgaaaac ggtggcagcg tcgtctacgt
     2401 caagaccggc ggcagcggcg tgcaggctgc ctttgaggcc gtgtccgtga ccacgggtga
     2461 ggagaacgac tactatatcg agatctccgg cgacgggctg gaggagggca tggaggtgcg
     2521 cagcagcgcc aacgccgagg aggccgaggt gggcggcggc agcagcggcg ccgacggggc
     2581 tgccggcggc atgggcggct ttgccgtcat gggcggcgcc atggcaatgc ccgacggcgg
     2641 cggtgcgccc ggcggtgggg gcggcggccc gatggggggc tgaccgatga cacaggatat
     2701 ttttgaaacc cgcgcgggcg gcgctgcgcc cggcgagccg ctgatcgcca tccgcgacct
     2761 ggtcaagacc tattatatcg gcaagcccaa cgagttggaa attttgcacg gcatcagcct
     2821 gaccgtctgg ccgggcgagt ttgtggccat cgtgggcgaa tcgggttccg gcaaatccac
     2881 catgatgaac atcatcggcg cgctggaccg ccccaccagc ggcacttatc atctggacgg
     2941 cgtggatgtg gaaaaggcca ccgatcccca gctgtccgcc atccgcaacc gcaagatcgg
     3001 ctttgtcttt cagaccttta acctcatcgg ccgcaccacc gcgcaaaaaa acgtcgagct
     3061 gcccatgctg tacgcgggcc tgcccgctcg ccagcgctcg gcccgggcca aagcgctgct
     3121 ggagatggtg ggcatggccg accgcgccag gcaccagccc agcgagctct ccggcgggca
     3181 gaagcagcgg gtggccatcg cccgcgccat ggcctgcgac ccggcgctgc tgctggccga
     3241 cgagcccacc ggggcgctgg actcccagac cagccgcacc gtgatggata tttttcacga
     3301 cctgcatgag cagcagggca agacaatcgt gctcatcacc cataaccctg agctcgccga
     3361 ggagtgcggg cgcatcctga ccctcaagga cggcaggatc atcagcgagc ggaaaggaag
     3421 tggcgcccgt gggactgctt gacaacatcc gcatggcctt tgccagc
//
LOCUS       DBMDYA010000256         3465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6178_length_3465_cov_1.509810, whole genome shotgun sequence.
ACCESSION   DBMDYA010000256 DBMDYA010000000
VERSION     DBMDYA010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_3465_cov_1.509810"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            715..957
                     /locus_tag="ACZPFK_07205"
     CDS             715..957
                     /locus_tag="ACZPFK_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406133.1"
                     /translation="MDLKEAIAGRIKELCAEKQLNASSLAYLCGMDRSTVYSILGVKS
                     KSPEVATIKKICDGLEITLGQFFSSPLFDALEQEIR"
     gene            complement(1548..2357)
                     /locus_tag="ACZPFK_07210"
     CDS             complement(1548..2357)
                     /locus_tag="ACZPFK_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR1406134.1"
                     /translation="MSLQYHLKIQEGDVAPYVLLPGDPKRVPIVASFWDEAHFVADNR
                     EHVTYTGVYKGVPISCTSTGMGCPSTAIAMEELARCGVKNFIRIGTCGTFQDYVKKGD
                     IQIFDSACRYDGTSYHYAPGPFPAVADHEVIEACIAAAKGMNKPYHVGTTRTQDTFYA
                     NYPDPGSSFNGFWQSRWKEFYPDLKRLNVMGGEMETSIVLVLTRIWGLRGGAMAVTLD
                     NIIESQEDEQGGREYEPEKALDHSEDNIKTLSLLGCEAIKKLYELDQASKG"
     gene            complement(2555..3091)
                     /locus_tag="ACZPFK_07215"
     CDS             complement(2555..3091)
                     /locus_tag="ACZPFK_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIR1406135.1"
                     /translation="MKKIKIAALVCCLALLTGLFAACGNDSSLSEPGSSSAASSLAAT
                     PAPSEEPKEETKTAFFDAHIVSAELLDEYDGKTPTEGGAFLKVEISVTNTMKKEIYLF
                     DNDFLLTWHEGVEAEPLPKFNDEMAPEEAKLEPGAATSYIYLYEVPAGTEEFTVLFQE
                     LFEGDVEGEIFKLPIELT"
     gene            complement(3248..>3465)
                     /locus_tag="ACZPFK_07220"
     CDS             complement(3248..>3465)
                     /locus_tag="ACZPFK_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1406136.1"
                     /translation="AGKIRLPFLSMKGIGESAAIAMEEACRQNESFLSVDEFQSISGV
                     SSSVIESLDAAGVFRDLPKSSQISLFG"
BASE COUNT          712 a         1036 c          962 g          755 t
ORIGIN      
        1 cacggtcttt gaggtgccgg tgtccgaggc gggcttgctc gccggcgccg ccgaggagct
       61 gtccccgccc ccgcaggcgg ccaggctgac cgccattgcc cgtgccaaga tcagtgataa
      121 aactttcttc atgtttagtg cctctctttc ctttctttat tcgatggagc ccccgcgcag
      181 agcgcaggga gttcccgcgt accacatcac agcgtgcccg gccgccgcct ttttccccgc
      241 ggcggagcgg acgccgaaaa tttgtgtcga cttgtcccct ttttaggccg caaatcacag
      301 gctttcgact tttatcgggt gaaagccgcg cctgtcggcg tcttcctcaa aaactgcgtc
      361 tactccacca gacctattcg ggattttctt cactacaaga tatatgtacc gcgaggggcg
      421 gtaagaacta tatccgcacc cgtcttgtcc attttttcag tttcggcctt gcgcctgcaa
      481 gaaagcgcaa caggcgcagg gggaagcatc ggtcgcccgg gcggcagtct tttttccctc
      541 tctttcgcgc cgtcccggat ttactataaa acagatcttt tgtctgtggg atatatccca
      601 caatatcaca ttttacattg tgggacaagt cccacaaagc aaatttgtac acttgttaca
      661 atatgttgga catatcgtac aaaacatgca caaagcagga aagggagaga tcagatggat
      721 ttgaaagaag cgattgccgg ccgcatcaaa gagctctgcg cggaaaagca gttaaatgcg
      781 agcagccttg cctatctctg cggcatggac cgctccacgg tctacagtat cctgggggtg
      841 aagagcaaaa gcccggaggt ggcgaccatc aaaaagatat gcgacggcct ggagatcacg
      901 ctgggacaat ttttcagcag tcccctgttc gacgcgctgg agcaggagat ccgatagacg
      961 cgcccctccc gcagctgctc cacacgcaaa aaggccccct ccctgcgaca cacttgagct
     1021 gtcgcaggga gggggccttg agcttgatac cgggcgcagc agggagccgc agccctgcgg
     1081 gcggcggatg ctctgctttc ggcgaacccg ccgcgcagaa agcagaaaat ggtttttgaa
     1141 aatcagcgat atcttagaat aaagaacagg cgccgcccgt ttttttgggc acgccctccc
     1201 gcccttttgg tcagaaagca ttcctctcgc catccggccc aaggccgccg ctatagggtt
     1261 cttcgcctca agaagagctg cggcaaagcg gctgaggggg ccgggcccta caaaaaagcg
     1321 gcgcttttcc gccgcccgcc atcgagcgcc atgccttatc cctccgccgc gtcttcatcg
     1381 cgttttctcc ccgcgcgcag aggagacggg atgcgcgcgc ccgggcgctt ttgccgtaaa
     1441 aaagccctgc cccctctgcc aaagaaattt ctttctcccc gacaaaaaaa catccggccc
     1501 gcaggccgga tgcaaaagag ctattaaaat gtaaaaacgc gcgccgatca gcccttgctc
     1561 gcctgatcca gctcgtacag ctttttgatg gcctcgcagc ccagcagaga cagggtcttg
     1621 atgttgtcct cgctgtggtc cagcgccttt tcaggctcgt actcgcggcc gccctgctcg
     1681 tcctcctgcg actcgatgat gttgtcgagg gtgacggcca tcgcgccgcc gcgcaggccc
     1741 cagatgcggg tgagcaccag tacgatgctg gtctccatct cgccgcccat gacgttcagg
     1801 cgcttcaggt cggggtagaa ctctttccag cgggactgcc agaagccgtt gaaagagctg
     1861 cccggatcgg ggtagttggc gtagaacgtg tcctgggtgc gggtggtgcc cacatgatag
     1921 ggcttgttca tcccctttgc cgccgcgatg caggcctcga tcacctcgtg gtccgccacc
     1981 gccgggaaag gcccgggcgc atagtggtaa gaggtgccat cataccggca ggcggagtcg
     2041 aagatctgga tatcgccctt tttgacgtaa tcctggaacg tgccgcaggt gccgatgcgg
     2101 ataaagtttt tgacgccgca gcgggccagc tcttccatgg cgatggcagt ggacgggcag
     2161 cccatgccgg tgctggtgca ggagatgggc acacccttgt acaccccggt ataggtgaca
     2221 tgctcgcggt tgtcggccac aaaatgggcc tcatcccaga agctggcgac gatgggcacc
     2281 cgcttggggt cgcccggcag cagaacatag ggcgcgacgt caccctcctg aattttcaaa
     2341 tgatactgca aagacattgg ttttcctcct tgaaggatgt tattccaggc ggtctcccgc
     2401 ccgttttgca cgagcggcgg cgcagcgcgc cgcacggtcg tccctctgct ctaatcatag
     2461 cctttgggac aagatttgta aagcgatatt ctctgtttgc agaagaaaag ggacaaaaac
     2521 acgccccggc tttgccgagg cgtgtttttg aggtttaggt cagttctatc gggagtttga
     2581 aaatttcgcc ttccacatcg ccctcgaaga gttcctggaa caaaacagta aactcttccg
     2641 ttcctgcagg gacctcatac agataaatat agctcgtcgc cgcgccgggc tccaacttcg
     2701 cttcttcagg agccatctca tcattgaact tcggcagagg ttccgcttcc acaccttcat
     2761 gccaggtcaa taaaaagtca ttgtcaaaaa gataaatttc ctttttcatg gtgttggtga
     2821 cgctgatctc cactttcagg aaagcgcccc cctcggtggg cgtcttgccg tcgtactcgt
     2881 ccagcagctc ggcgctgacg atatgggcgt cgaagaaagc ggttttggtc tcctctttcg
     2941 gctcctcgct gggcgccggg gtggccgcca gggacgacgc cgcgctggag gaacccggct
     3001 cagacaggga cgaatcgttg ccgcaggccg caaagaggcc ggtcagcagt gccagacagc
     3061 agaccagggc ggcgatcttg atcttcttca tgtcgtatcc tctctttctc attctcacct
     3121 cgcggcaggg ccgcgacgct gcttttctga taccgctatc atatcacaga aacagcaaag
     3181 tgtgcaaatc aggaaaatca gtctgatgtg actgattttg ttactttttt gatctcaaac
     3241 cgccctctca gccgaaaagg ctgatctggc tgctcttggg caggtcccga aagacgcccg
     3301 ccgcatccag cgactcgatg accgacgagg acacgcccga gatggactgg aactcgtcca
     3361 ccgacaaaaa gctctcgttc tgccggcagg cctcctccat ggcgatggcc gcggattcgc
     3421 cgatgccttt catcgagagg aacggcaggc ggattttgcc ggcct
//
LOCUS       DBMDYA010000257         3465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6181_length_3465_cov_1.392687, whole genome shotgun sequence.
ACCESSION   DBMDYA010000257 DBMDYA010000000
VERSION     DBMDYA010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6181_length_3465_cov_1.392687"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(286..1725)
                     /locus_tag="ACZPFK_07225"
     CDS             complement(286..1725)
                     /locus_tag="ACZPFK_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406137.1"
                     /translation="MKKELSNGRMAGLCLGEFARAVLGGLIVTYTLKFFNVTPASGLP
                     LLLPAGMMGLLRGVGVIFDAITDPWVASLSDRRPPEKGRRIPFMRWSALPYALTCLLI
                     FFPPSGSVSWVNVVWVVAMLLCYYMSSTLYCVPFMALQQEVVTDTRRRVFFYTVNSLM
                     FVLGSAVIYALPVMVGAFEGGMAPVAAWRAALAIFAVVGAVCALVPCFVIREQDYVES
                     KSCYMPLLQSLKTTLRYKNFAVMTVGYLIMQAGFAFFNGALLFYIDTLLGLKESFATI
                     VLAISIVVGICTYPLVNLLVRRFGKKPLLLAACCAYVLIYLGIFFYAPISAAIGSGPI
                     GPGLLASLAGQGAAVGDVAVALLIGVLVAFPIACTNILPPAAFADMAQYDRIRSGEDR
                     ADMFVAARQFVTKLSQAAVTAIVSYTMYLGSTDSYPTHGGVRATALIAACCLVFAVAV
                     YAFYDDRSVVAAIDRFNAEKKARDASGAK"
     gene            complement(1722..2696)
                     /locus_tag="ACZPFK_07230"
     CDS             complement(1722..2696)
                     /locus_tag="ACZPFK_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1406138.1"
                     /translation="MAYEIDEIRKVTLGGVKQKIHIRGERLRNPVLLFLHGGPGISNR
                     DSVINREKDLCDAFTIVAWDQRGTGGSYWGVKKETLNVDQLVSDAAELVEYLCRELGK
                     EKLFVWGGSWGTELGTYLCYRHPQHIAGYLGSGQMVNGVLNEERSYDFALAEAKKAGD
                     AESVRILEEIGRPVGGCYREIFKGMMAQRRIMKKYGGHTMKKGTYWTDTAKPLLKSKE
                     FSFTDKIGLALGYKKCLTYMWPTTVLCDFMTQCTRFEMPYYIFQGVHDNNTPSSLVQD
                     YYDAIEAPDKDLIWFEHSAHGPLGEEPERYKALLREKFLKRVPEVAAR"
     gene            complement(2901..3434)
                     /locus_tag="ACZPFK_07235"
     CDS             complement(2901..3434)
                     /locus_tag="ACZPFK_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1406139.1"
                     /translation="MNFWGHLSTINHHKRLVLRYCFRLGLYRQGLCHDLSKYSPVEFW
                     AGVRYFQGDHSPNEAEREDKGYSAAWLHHKGRNKHHLEYWIDYDPGAGHAMTGMRMPE
                     RYVAEMVCDRIAASRTYLKEAYTDRAPWEYYERSRDHYLLHPETRALLERLLAMLRDE
                     GEEKTFAFLRHEVLQKR"
BASE COUNT          647 a         1102 c         1124 g          592 t
ORIGIN      
        1 ccggcctccg ccccggcccc cgccgtgccg ccgggcggtt ttcggcgcgc gcccatggag
       61 gccggtgggg gcatggattt gggccggcgg gccaaaaagg gcggctcccg cttcgtgcgc
      121 tcatcgcccc gtctttgcgg ggacggacct cctttcccac tcctttcccg ttcagcgcaa
      181 agagggggga catccagaat acaaaaagaa cgccccgggc ctgacaaaag gccggggcgt
      241 tctttttagt tgggtggaaa aagcgccgaa aagtgcggca cggcgtcatt tggcgccgga
      301 ggcgtcccgc gccttttttt cggcgttgaa gcggtcgatg gccgccacca ccgagcggtc
      361 gtcgtaaaag gcgtagaccg ccaccgcaaa aaccaggcag caggccgcga tcagcgccgt
      421 ggcccgcacg cccccgtggg tggggtaact gtcggtggag cccaggtaca tggtatagga
      481 gacgatggcc gtcaccgcgg cctgggagag cttggtcacg aactggcggg ccgccacgaa
      541 catgtccgcc cggtcctctc cggagcggat gcggtcgtac tgggccatat ccgcaaacgc
      601 ggcgggcggc aggatgttgg tgcaggcgat gggaaaagcc accagcaccc cgatgagcag
      661 cgcgacggcc acgtccccca ccgccgcgcc ctggccggcc agcgaggcca gcagccccgg
      721 gccgatgggg ccgctgccga tagcggcgga gatgggcgca tagaaaaaga tgcccagata
      781 gatgagcaca taggcgcagc aggcggccag cagcagcggc tttttgccga agcggcgcac
      841 cagcaggttg accagcggat aggtgcagat gcccaccacg atgctgatgg ccagcacgat
      901 ggtggcaaag ctctctttca ggcccagcag cgtgtcgatg tagaacagca gcgccccgtt
      961 gaaaaaggca aagccggcct gcatgatcag gtagcccacg gtcatgaccg caaagttttt
     1021 gtagcgcagg gtggtcttca ggctttgaag cagcggcatg tagcagcttt tggactccac
     1081 atagtcctgc tcgcggatga cgaagcaggg gacgagggcg cagaccgcgc ccacgaccgc
     1141 aaagatggcg agcgcagcgc gccaggcggc aacgggggcc atgccgccct caaaagcgcc
     1201 caccatcacc ggcagggcgt agatgacggc gctgcccagc acgaacatca gggagttgac
     1261 ggtgtagaaa aagacccggc gcctggtgtc ggtgacgacc tcctgctgca gggccataaa
     1321 gggcacgcag tagagggttg aggacatgta gtagcacagc agcatcgcca ccacccagac
     1381 cacgttgacc cagctcaccg accccgaggg agggaagaag atcagcaggc aggtgagcgc
     1441 atagggcagg gcggaccagc gcataaaggg gatgcggcgc cccttttccg gcgggcggcg
     1501 gtcgctgagc gaggccaccc aggggtcggt gatggcgtcg aagatcaccc ccaccccccg
     1561 cagcagcccc atcatgcccg cgggcagcag caacggcaga ccggaggctg gggtgacgtt
     1621 gaaaaacttc agcgtgtagg tgacgatgag gccgcccagc acggcgcggg cgaactcgcc
     1681 caggcacagg ccggccatgc gtccgttaga gagctctttt ttcatcgcgc agccacctcc
     1741 ggcacccgtt tgagaaactt ttcccgcagc agggccttgt accgctctgg ctcctcgccc
     1801 agcggcccgt gggccgagtg ctcaaaccag atcaaatcct tgtccggcgc ctcgatggcg
     1861 tcgtaataat cctgcaccag gctgctgggg gtgttgttgt cgtgcacccc ctgaaagatg
     1921 taatagggca tctcgaagcg ggtgcactga gtcataaagt cgcagagcac ggtggtgggc
     1981 cacatgtagg tcagacactt tttgtacccc agcgccagcc cgatcttgtc ggtaaaagaa
     2041 aattccttgc ttttcagcag cggctttgcc gtgtcggtcc agtaggtgcc ctttttcatg
     2101 gtgtggccgc cgtatttttt catgatgcgc cgctgggcca tcatgccctt gaagatctcg
     2161 cggtagcagc cgcccaccgg ccggccgatc tcctccagaa tgcgcaccga ttcggcgtcg
     2221 cccgcctttt tggcctcggc cagggcaaag tcgtagctgc gctcctcgtt gagcacgccg
     2281 ttgaccatct ggccgctgcc cagatacccc gcgatgtgct gcgggtggcg atagcacaga
     2341 taggtgccca gctcggtgcc ccagctgcct ccccagacga agagcttctc cttacccagc
     2401 tcgcggcaga gatactccac cagctcggcg gcgtccgaga ccagctggtc cacgttgagg
     2461 gtctcctttt tgacccccca gtacgaaccg ccggtgccgc gctggtccca ggccacgatg
     2521 gtgaaagcgt cgcacaggtc tttttcccgg ttgatgacgc tgtcccgatt gctgatgccg
     2581 gggccgccgt gcaaaaacag cagcaccggg ttgcgcagcc gttcgccgcg gatgtgaatc
     2641 ttctgcttga cgccgcccag cgtcaccttg cggatctcgt cgatctcata ggccatgagg
     2701 gacccctcct tttgtcgcgc cgcgcgccgc cgcacccggc cgggcaaagc ggacccgggc
     2761 ggtcgcccgc ccgcgccgcg cgctgttgtt ggccttattg taaactgttt tgagggcccg
     2821 cgcaagcgca gaggtgaaac agacaaaaac agcgccgtcc ccctgacgga ggggaacggc
     2881 gccggggaac agggcggcgg tcagcgcttt tgcagcacct catgacggag aaaggcaaag
     2941 gtcttttcct ccccctcgtc ccgcagcata gccagcaggc gctccagcag ggcgcgggtc
     3001 tcggggtgca gcagatagtg gtcgcgagag cgctcatagt actcccaggg ggcgcggtcg
     3061 gtataggcct ctttcagata ggtgcggctg gccgcgatgc ggtcgcagac catctccgcc
     3121 acatagcgct ccggcatccg catgccggtc atggcgtggc ccgcgccggg gtcataatcg
     3181 atccagtatt ccagatggtg cttgttgcgc cccttgtggt gcagccaggc ggcgctgtag
     3241 cccttgtcct cccgctccgc ctcgttgggg ctgtggtcgc cctgaaaata tctcactccg
     3301 gcccagaact ccaccgggct gtatttcgag agatcgtggc agaggccctg gcggtagagg
     3361 cccagccgga agcagtagcg cagcaccagc cgcttgtgat ggttgatggt ggacagatgg
     3421 ccccaaaaat tcatgtgcgg catcctttct gaaagcattt tcaag
//
LOCUS       DBMDYA010000258         3464 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6184_length_3464_cov_2.089801, whole genome shotgun sequence.
ACCESSION   DBMDYA010000258 DBMDYA010000000
VERSION     DBMDYA010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6184_length_3464_cov_2.089801"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1107
                     /locus_tag="ACZPFK_07240"
     CDS             136..1107
                     /locus_tag="ACZPFK_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406140.1"
                     /translation="MEKTKKFAVSSLKAITLPVIVYLIFKIIRPATFGSPEALYIIAQ
                     QAMMVTIMSLGMCCNMTIGIWDFSPGSIVTVVGLICGHAYGEYGFAGMVIAGILSGML
                     IGLLNATVYTVLKVPSVVVTVGMLLIYESFGSVYKGGLGVTIDREGAILGQAPWIFLI
                     TALMFVILYVIYNKTKIGFNVKAVGNNELIAKTAGINPRKVKFLAFFIAGCFFGIAGL
                     INLSYGTALSPAKNMGTMSVTFDAMMAGFIAIYLSGVVNRVVGIVIGTFTMKLVSAGL
                     VAIGVEGTWQKVIIGVFLLFFIGFTQIKAQVSEWLGRIKRAKKTAAG"
     gene            1152..>1910
                     /locus_tag="ACZPFK_07245"
     CDS             1152..>1910
                     /locus_tag="ACZPFK_07245"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1406141.1"
                     /translation="MYFGADYYPEHWPKERWAVDAKLMKEAGFNLIRVAEFAWAKLEP
                     AEGHWDFGWLDEALDVFYKEGIQVVMGTPTATPPKWLMDKHPEIYQVDENGVVQGFGS
                     RRHYCYNSEIYREYSEKIVEKMARHYAGHPAVAAWQIDNEFTCGDGLYCYCEECRQKF
                     IVWLKKKYGSLDALNEAWGTVFWSQTYTDWDQIIVPRKSQAAMFRNNGHNPGLNLDYS
                     RFQSDAIVDYCALQSRILRRWTKLPITHNVVSELY"
     assembly_gap    1911..1920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1921..3168
                     /locus_tag="ACZPFK_07250"
     CDS             <1921..3168
                     /locus_tag="ACZPFK_07250"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1406142.1"
                     /translation="ELYDYYKMGEQIDVATYDNYPASPWKSFLGGAVYDPAFSIDIER
                     GAKNKNVWVMEQQSGPCGWNVLGSTPRPGQLKKWTYQALAHGAEAIVYFRWRACLFGT
                     EEYWYGILDHDGVPRRRYEEIKAIGKTFQKLSAYNAESKVSAEILMIRSFEQQWSHAF
                     QEHNWNFNYRGYLKNLYYGLYANNYNLDISSEATDFSGYKVVMAPAFNLMSDELKAKF
                     EKYVAEGGNLVVTFRSGTKNMDNSMTERTLPGYFKEVAGIELEEFDSLNDCAVEVEGA
                     FGRGRASIWADIIKPVTAKTVATYAGEYYAGKPAVTVNEYGKGKCYYVGCDLDEAATV
                     ALMDYIASQCGVSPAIKNAPQGVEVVKKVASDGSEFYFVMNFAGKEETVEFPCAVKDI
                     TTDEVLGNKVTLAVYGSAAVARA"
     gene            3235..>3464
                     /locus_tag="ACZPFK_07255"
     CDS             3235..>3464
                     /locus_tag="ACZPFK_07255"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1406143.1"
                     /translation="MYFGADYYPEHWPKERWAVDAKLMKEAGFNLIRVAEFAWAKLEP
                     AEGHWDFGWLDEALDVFYKEGIQVVMGTPTATP"
BASE COUNT          798 a          796 c         1027 g          833 t
ORIGIN      
        1 ttgaaagtaa agcggggctg ccttttctga aatgataagg acctgccgcg ggcaggccgc
       61 ccgcggcagg ttttctcttt cgctccatta tttctcgtcc gttcacttat tttacttgga
      121 tatggaagtg atatcgtgga aaagacaaag aaattcgcag tttcctcgct gaaagccatc
      181 acactaccgg tcattgttta tctgatcttc aagatcatcc ggcctgctac ttttggctct
      241 cccgaggcac tttatatcat cgcccagcag gcgatgatgg tcacaatcat gtctttgggt
      301 atgtgctgca acatgaccat cggtatctgg gactttagcc cgggttcgat tgtaactgtg
      361 gtgggcctga tttgtggcca tgcttatggc gagtacggct ttgccgggat ggtgattgcg
      421 gggattctct ccggcatgct gattggtttg ctgaatgcta ctgtttacac cgtgttgaaa
      481 gtcccctccg tagtggtcac ggttggtatg ttgttgatct acgaatcttt tgggtcggtg
      541 tataagggcg gcctgggggt cacgattgac cgggaagggg cgattttagg ccaagccccc
      601 tggatatttt tgatcaccgc gctgatgttt gtgatcctct atgtgattta caataaaaca
      661 aagattggct ttaacgtcaa ggcggtgggc aataacgagc tgattgctaa aacggcaggg
      721 atcaatcccc gcaaggtaaa atttttggct ttctttatcg ctggctgctt ttttggaatc
      781 gcggggctga tcaatctctc ctacggcact gcgctctcac cggctaaaaa tatgggcacg
      841 atgagcgtca cttttgacgc gatgatggcg ggctttattg cgatctatct gtcgggggtc
      901 gtcaatcggg ttgtgggcat cgtaatcggc actttcacta tgaaactggt ctccgccggg
      961 ctcgtcgcta ttggcgtgga ggggacctgg cagaaagtca ttatcggcgt atttttgctc
     1021 tttttcatcg gctttactca aattaaggca caggtcagtg aatggctggg gcgtattaaa
     1081 agggcaaaaa agacagctgc aggctaaaaa gcttgtcaag cgggaagatg acaacagaaa
     1141 gaggtgtatt tatgtatttt ggcgcagatt attatccgga gcattggccc aaggagcggt
     1201 gggcggtgga cgcaaagctg atgaaagagg cagggttcaa cctgatccgg gtggcggagt
     1261 ttgcctgggc gaaattagag cctgccgagg gccactggga ttttggctgg ctggatgaag
     1321 cgctggatgt tttctataaa gaggggatcc aggtggtgat gggcaccccc acggccacgc
     1381 cgcccaaatg gctgatggat aagcacccgg aaatctatca ggtggacgaa aacggcgtgg
     1441 tgcagggctt tggctcccgc cggcattact gctacaacag cgagatatac cgggagtact
     1501 cggaaaagat cgtggaaaag atggcgcggc actatgcggg tcatccggcg gtggccgcct
     1561 ggcagatcga caacgaattc acctgtggcg acggactgta ctgctattgc gaggagtgca
     1621 ggcagaagtt tatcgtctgg ctgaagaaaa aatacggcag cctggacgcg ttgaacgagg
     1681 cttgggggac tgtgttctgg agccagacct ataccgattg ggaccagatc attgtgccgc
     1741 gcaaatccca agcggcaatg ttccgcaaca atggccataa ccccgggctg aatctggatt
     1801 attcccgctt ccaatccgat gccatcgtgg actattgcgc tcttcagagc cgcattttgc
     1861 gccgttggac gaagctgccc atcacccaca atgtggtaag tgaattgtac nnnnnnnnnn
     1921 gaattgtacg attattataa gatgggcgag cagatcgacg tggccaccta tgataattac
     1981 cccgccagcc cctggaagag ctttttgggt ggtgcggtgt atgatcccgc tttttccatt
     2041 gacatcgagc gcggcgctaa aaacaaaaat gtttgggtta tggaacagca aagcggcccg
     2101 tgcggctgga atgtgctggg cagcacaccg cgccccggcc agctcaaaaa gtggacctat
     2161 caggctctgg cgcacggcgc cgaagctatt gtctatttcc gctggcgtgc ctgtctgttc
     2221 ggcaccgaag agtactggta cggtattctg gaccatgacg gtgtgccccg ccgccgctat
     2281 gaagagatca aggcgatcgg aaagaccttt caaaagcttt ccgcctacaa tgcagagtcc
     2341 aaggtaagcg cagagatctt gatgatccgc tcgttcgagc agcagtggag ccacgcattt
     2401 caggagcata actggaactt caactataga ggatatctga aaaacctcta ttatggcctt
     2461 tatgccaaca actacaacct ggacatctcc agcgaggcca ctgacttttc cggttataag
     2521 gtggtgatgg ctccggcgtt taatctgatg agcgacgaac tgaaagccaa gtttgagaag
     2581 tacgtagccg agggcggcaa tttggtagtc actttccggt ctggcaccaa aaatatggac
     2641 aattctatga ccgagcgcac cttgccgggt tactttaaag aggtggccgg cattgaattg
     2701 gaagagtttg attccctcaa tgattgcgcg gtagaagtcg agggcgcgtt tggcagaggc
     2761 agggcctcga tctgggcgga cattatcaag ccggtcaccg ccaagacggt cgcgacctat
     2821 gctggcgaat actatgcggg caagccggcc gtcactgtca acgagtacgg caagggcaaa
     2881 tgctattatg tgggctgcga cctagacgag gcggctaccg tggcattgat ggattatatc
     2941 gcgagccagt gtggagtgag cccggcaatc aagaatgcgc ctcaaggtgt tgaagtggtc
     3001 aaaaaagtcg cttccgacgg cagcgagttc tattttgtca tgaattttgc cggcaaggag
     3061 gaaactgtcg aattcccgtg cgcggtgaaa gacatcacta ccgatgaggt actgggtaac
     3121 aaggtgacct tggcggttta tggcagcgcg gcggtcgcga gagcttgatt acaaaaatca
     3181 gagaaagagg gtccccgacg gacccccttt ctttctaaaa ggagggtatt gaaaatgtat
     3241 tttggcgcag attattatcc ggagcattgg cccaaggagc ggtgggcggt ggacgcaaag
     3301 ctgatgaaag aggcggggtt caacctgatc cgggtggcgg agtttgcctg ggcgaaatta
     3361 gagcctgccg agggtcactg ggattttggc tggctggatg aagcgctgga tgttttctat
     3421 aaagagggga tccaggtggt gatgggcacc cccacggcca cgcc
//
LOCUS       DBMDYA010000259         3448 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6225_length_3448_cov_1.504332, whole genome shotgun sequence.
ACCESSION   DBMDYA010000259 DBMDYA010000000
VERSION     DBMDYA010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6225_length_3448_cov_1.504332"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(386..1105)
                     /locus_tag="ACZPFK_07260"
     CDS             complement(386..1105)
                     /locus_tag="ACZPFK_07260"
                     /inference="COORDINATES: protein motif:HMM:NF046770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR1406144.1"
                     /translation="MRNRIGSILWGLAFVVAGVGFAGNAFGVWDFNLFFDGWWTLFII
                     VPAVISMIQSGPHTGNIICALIGVVLLLGAQDILDRHLMGKLFLPVLLVVIGISILLG
                     GRRRVATGTAQAGMGGADAAGYTAAQSDRGGVPNYSGVFSGCDVRWPAEPFHGANITA
                     VFGGVDLDLRDAVFEGDVVVNATTIFGGADLKVPPHVRVKVTGTPVFGGVDNRADGPA
                     SEGTPVLYLNASCVFGGIDIK"
     gene            complement(1343..1531)
                     /locus_tag="ACZPFK_07265"
     CDS             complement(1343..1531)
                     /locus_tag="ACZPFK_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR1406145.1"
                     /translation="MEQKRLYKDRQHQMLCGVCAGFAEYLNIDVTLVRLLMVLLGLTS
                     IGVIFYIIAAVVMPEKPI"
     gene            complement(1535..2458)
                     /locus_tag="ACZPFK_07270"
     CDS             complement(1535..2458)
                     /locus_tag="ACZPFK_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406146.1"
                     /translation="MKSFYKVMAIIAACLLVLGVLLSGVAFACGVSAEATGGVIRLGR
                     YIQIPVGWGHHDWYWDWDDGPDDGYDGYHYDSGSSGASVSGTLPASSAAAQGDKTMED
                     KSGIRKIELELGGGSFKVVSGDSFKVDITGLPADKVYSGFDGDEWKVEVPHGKTSLNM
                     YHDVRGTVTIPADCVPEVLEIEIGAGELIVEGLTATRSAELSVGAGTMTVTDCTLANA
                     EIDCGMGELVFDGVLTGRGSIDCGMGNVTVTTIGRADDYGAKVECGMGNVEFNGLRWA
                     AVGSGTANAGAANYFDIECGMGNVEFNIREE"
     gene            complement(2478..3104)
                     /locus_tag="ACZPFK_07275"
     CDS             complement(2478..3104)
                     /locus_tag="ACZPFK_07275"
                     /inference="COORDINATES: protein motif:HMM:NF046861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1406147.1"
                     /translation="MTKREFLMALNDELYPLSEDERSAAIKYYSDYIDDAGEENLDEV
                     LAELGSPAQVAASIKADGELGRSVPPAGSYGAASQPQQGPEVRRSGLPVWAVVLLVVL
                     LSPVWMPLLGAAVGLVVGVIGIFIGLIVAGAALVLSGVFLAVMGLFSIFGSPLVGLLV
                     LGVGFILAAVGLLLVMASVFLCATVIPAMVRGIVKICRMPLRGRQSNG"
     gene            complement(3101..3418)
                     /locus_tag="ACZPFK_07280"
     CDS             complement(3101..3418)
                     /locus_tag="ACZPFK_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1406148.1"
                     /translation="MAFITGSTLLDAIVLSVAAREGTYGYKITQDVRGVMEVSESTLY
                     PVLRRLQKDGCLTTYDQAFAGRNRRYYKITPAGSQRLNETLAEWEEYKQKIDRVFYGG
                     NET"
BASE COUNT          608 a         1220 c         1048 g          572 t
ORIGIN      
        1 ccctgcggga gccgccctcc cggggaggga gacagcgggg cggcaagggt gcgcccgagg
       61 agcgccgggc gcggcgcagt gcccaaaaag gaaaatcgct gcgcgccctg gggcaagccc
      121 ctctcacata gcctggggcc gcccgctccc ctgcaaggtg agtcatggag tgcgtttttg
      181 gggataggag cgcgcagccc aagacatggc ccaaagcgcg ctgttttgaa gaagagcgtg
      241 cagcccgcgg cgcgctgttc ctcctccccc tgcaaagtgg ctgcatggcc cgcgccctcc
      301 gccctgcggg cccgccccgc ccgcacagag ataaaagaac agcccccgct aggcgggggc
      361 tgttctttta tgggccggtc gccctttatt tgatgtcgat accgccaaag acgcagctgg
      421 cgttcaggta gagcaccggg gtgccctccg acgccgggcc gtcggcccgg ttgtccacgc
      481 cgccgaagac cggtgtgccg gtgactttca cccgcacatg gggcggcact ttcaggtccg
      541 cgccgccgaa gatggtggtg gcgttgacca ccacgtcgcc ctcgaacacc gcgtcccgca
      601 ggtccaggtc caccccgccg aagacggcgg tgatgttcgc gccgtggaac ggctccgccg
      661 gccagcgcac gtcgcagccg ctgaagacgc ccgaatagtt cggcacgccg ccgcggtcgc
      721 tctgagccgc ggtatagccc gccgcgtccg cgccgcccat gcccgcctgg gccgtgccgg
      781 tggccacgcg gcggcggccg cccagcagaa tcgagatgcc gatcaccacc agcagcacgg
      841 gcaggaagag cttgcccatc aggtggcggt ccaggatatc ctgcgccccc agcagcagca
      901 ccacgccgat cagcgcgcag atgatgttgc cggtgtgcgg cccgctctgg atcatactga
      961 taacggccgg tacgatgatg aacagggtcc accagccgtc gaaaaagaga ttgaagtccc
     1021 agacgccgaa cgcgttgccc gcaaagccca cgcccgcgac cacaaacgcc aggccccaca
     1081 aaatactgcc gatccggttt ctcatagtgt tctcctttgt tcaaaagctc tttttgtgaa
     1141 aagcgcagtg atggcgggga atggctggtt ttgcccggca gggcaaaacc gcacggcatc
     1201 ggctgtccgc ggaaaaagtt cggctgcaag ggcggcggag cgctgcgagc cctgttggct
     1261 tgcaaacggc gccgacgcgg cagacgtgct ttttccgttg ggagcaggcg acaatcgtcc
     1321 cctatcggaa ctgcgcgaaa ggtcagatgg gtttttccgg catcacgacc gccgcgatga
     1381 tgtaaaagat cacgccgatg gaggtcagcc ccagcagcac catcagcagg cgcaccagcg
     1441 tgacgtcgat gttgagatac tcggcaaagc ccgcgcagac gccgcagagc atctggtgct
     1501 gccggtcctt ataaaggcgt ttttgttcca caggtcattc ctccctgata ttaaattcga
     1561 cattgcccat gccgcactcg atgtcgaaat agttggcggc cccggcgttg gcggtgccgc
     1621 tgcccacggc ggcccagcgc aggccgttga actccacgtt gcccatgccg cactccacct
     1681 tggcgccgta gtcgtcggcc cggccgatgg tggtgacggt cacgttgccc atgccgcagt
     1741 cgatggagcc gcggccggtg aggacgccgt caaagaccaa ctcgcccatg ccgcagtcga
     1801 tctcggcgtt ggccagcgtg caatcggtca cggtcatggt gcccgcgccc accgacagct
     1861 ccgccgagcg ggtggccgtg aggccctcca cgatcagctc gcccgcgccg atctcgatct
     1921 ccagcacctc gggcacgcag tccgccggga tggtcaccgt gccgcgcacg tcgtggtaca
     1981 tgttcagcga ggtcttgccg tggggcacct ccactttcca ctcgtcgccg tcaaagccgc
     2041 tgtagacctt gtcggcgggc aggccggtga tgtccacctt gaagctgtcg ccgctcacca
     2101 ccttgaagct gccgccgccc agttccagtt cgatcttgcg gatcccgctc ttgtcctcca
     2161 tcgtcttgtc cccctgtgcg gccgcggagc tggcgggcaa ggttcccgag accgatgcgc
     2221 cgctgctgcc gctgtcgtaa tgatagccgt cgtacccgtc gtcgggaccg tcgtcccaat
     2281 cccagtacca gtcgtggtgg ccccagccca ccgggatctg gatgtagcgc cccaaccgga
     2341 tcaccccgcc ggtcgcctcg gcggacacgc cgcaggcgaa cgccaccccg gagagcagca
     2401 cccccagcac cagcaggcag gcggcgatga tcgccatcac cttataaaaa gatttcatgc
     2461 cctgtccctc cttttcttca cccgttgctc tgccggccgc gcagcggcat gcggcagatc
     2521 ttcacgatcc cgcgcaccat ggccgggatc accgtcgcgc acaggaacac cgaggccatc
     2581 accagcagca ggcccacggc ggccaggata aagccgacgc ccaataccag cagccccacc
     2641 agcgggctgc cgaagatgga gaagagaccc atcacggcca gaaacacgcc cgacagcacc
     2701 agtgccgcgc cggcgacgat gaggccgatg aagatcccga tgacccccac caccagcccg
     2761 accgccgcgc ccagcagggg catccagacc ggcgagagca gcaccaccag caacaccacg
     2821 gcccacaccg gcaggccgct gcgccgcacc tccggcccct gctggggctg cgaggccgcg
     2881 ccgtaggagc ccgcgggagg cacgctgcgg cccagctcgc cgtccgcctt gatgctggcc
     2941 gccacctgcg ccgggctgcc cagctcagcc agcacctcgt ccaggttctc ctcgccggcg
     3001 tcgtcgatgt agtcgctgta atatttgatc gcggcgctgc gctcatcctc cgacaggggg
     3061 tacagctcat cgttcagcgc catcaagaac tccctcttgg tcatgtctcg tttcctccat
     3121 aaaacacacg gtcgattttt tgtttatatt cttcccactc ggcgagcgtc tcgttcagcc
     3181 gctggctgcc cgccggggtg atcttgtaat agcgccggtt cctgccggca aacgcctggt
     3241 cgtaggtggt caggcagccg tctttctgca gccgccgcag caccgggtaa agggtggact
     3301 cggacacctc catcacgccg cgcacgtcct gggtgatttt gtacccgtag gtcccctccc
     3361 gcgcagccac cgacaacacg atggcgtcca gcagggtgga gccggttata aacgccatgc
     3421 cattcacctc tgtcctatgt tatatcaa
//
LOCUS       DBMDYA010000260         3446 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6232_length_3446_cov_1.387444, whole genome shotgun sequence.
ACCESSION   DBMDYA010000260 DBMDYA010000000
VERSION     DBMDYA010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6232_length_3446_cov_1.387444"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="eno"
                     /locus_tag="ACZPFK_07285"
     CDS             <1..911
                     /gene="eno"
                     /locus_tag="ACZPFK_07285"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1406149.1"
                     /translation="LPLYRYLGGVNGNRLPVPMMNILNGGAHAANTVDVQEFMIMPAG
                     AESFREGLRWCTEVFHALAALLKANGLATSVGDEGGFAPDLAGDEEAIEYILEAVRRA
                     GYEPGKDFVLAMDAASSEWKSGIQGEYLLPKSGKRFTSAQLVEHWKGLCDKYPIYSIE
                     DGLDEEDWEGWQLMTRGLGAKIQLVGDDLFVTNTERLAKGIRLGCGNSILIKLNQIGS
                     VSETLEAIKMAHRAGYTAIASHRSGETEDTTIADLAVALNTCQIKTGAPSRSERVAKY
                     NQLLRIEQALGSNAVYPGFEAFGRRG"
     gene            1117..2247
                     /gene="tgt"
                     /locus_tag="ACZPFK_07290"
     CDS             1117..2247
                     /gene="tgt"
                     /locus_tag="ACZPFK_07290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1406150.1"
                     /translation="MYELIKTEHTARRGRFKTVHGTVETPAFMNVATAAAIKGGLSAF
                     DLREIKCQVMLCNTYHLHLRPGDELVRDMGGLHRFTRWDGPILTDSGGFQVFSLASLR
                     KIREEGVTFNSHIDGRKIFMGPEESMRIQANLGSTIAMAFDECIKNPAEHGYAKASCA
                     RTVRWLKRCKQEMARLKREGLAVNPDQLLFGINQGCCFDDLRVEQMKEIAELDLDGYS
                     IGGLAVGEPTEEMYRVITVVEPHMPKDKIRYLMGVGTPGNILESIARGVDLFDCVMPA
                     RNARHGHFNTWGGIINIRNAKYERDESPIDPQCDCPVCRNFSRSYIRHLFKADEILGL
                     RLGVMHNLYFYNTLLERCREALDAGNFEDFRKKYSKILDSRI"
     gene            2337..2648
                     /gene="yajC"
                     /locus_tag="ACZPFK_07295"
     CDS             2337..2648
                     /gene="yajC"
                     /locus_tag="ACZPFK_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1406151.1"
                     /translation="MFPIFLTTGSDATFGIIGTILPFALVLVVMYFLMIRPQKKKDKQ
                     LQDLRNSLEIGDNVTTIGGIVGRVVAIKDETIVLETGTERNKVRFKRWAVQDVEKLVV
                     E"
     gene            2711..3127
                     /locus_tag="ACZPFK_07300"
     CDS             2711..3127
                     /locus_tag="ACZPFK_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR1406152.1"
                     /translation="MVKKAGAVKKSERPLAPALIFSFFTAVALTLVQFALVSSVMCRV
                     DVSPNLLGPLSTIMVCISALAAGTALAFSIGSKGMLLGFAEGAVFYLILMAAAFLQGN
                     FDFTNMAFVKLVAMCAAGAVGGYLGVGLAERKRRKH"
     assembly_gap    3141..3150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          722 a          984 c         1058 g          672 t
ORIGIN      
        1 ggctcccgct ttaccgctat ctggggggcg tcaacggcaa caggctgccg gtgcccatga
       61 tgaacatcct gaacggcggc gcacacgccg caaacacggt ggatgtacag gaatttatga
      121 tcatgcccgc cggggccgaa agctttcgcg aggggctgcg ctggtgcacc gaggtattcc
      181 acgcgctggc cgccctgctg aaagccaacg ggctcgccac ctcagtgggg gatgagggcg
      241 gctttgcgcc ggaccttgcc ggcgatgagg aggcgattga atatattctt gaggccgtcc
      301 gacgggctgg gtatgaaccg ggaaaagact ttgtactggc aatggacgcg gcctccagcg
      361 agtggaaaag cggaatccag ggcgaatacc tgctgcccaa aagcggcaag cgcttcacct
      421 ccgcccagct ggtcgagcac tggaaaggcc tctgcgacaa atacccgatc tattccattg
      481 aggacggcct tgacgaagag gattgggagg gctggcagct gatgacccgg gggctgggcg
      541 caaagatcca gctggtggga gacgatctgt ttgtcaccaa tacagaacgc ttggccaagg
      601 ggattcgtct gggctgcggc aactcgatcc tgatcaagct gaatcagatc ggctcggtgt
      661 ccgagacgct ggaggccatc aaaatggccc acagggccgg ctacaccgcc atcgcctccc
      721 atcgctccgg cgagacggag gacacgacga tcgccgatct ggcggtggcg ctgaacacct
      781 gccagatcaa gaccggcgcg cccagccgga gcgagcgggt ggcaaagtac aatcagctgc
      841 tccgcatcga gcaggctctt gggtcaaacg ccgtctatcc cggttttgag gcctttggcc
      901 gcagaggcta aagggctccg gcacaaaaaa gagcctcagg gctgccggcg cttttggggg
      961 cgtgcccggg ccgcccacgt tgactttcgg cggcaaagcg gctataataa acaaaaagat
     1021 caaaaggcgg tgctgaggag cggttccccg gcgccgccca ttttttggta tccgggcagg
     1081 gcccccacgc tctgcataaa agagagggta agagatatgt acgaactgat caaaacggag
     1141 cacacggcgc gccgaggccg ttttaagacc gtacacggca cggtggagac gccggccttt
     1201 atgaacgtcg ccaccgccgc cgccatcaag ggcgggctat ccgcgttcga cctcagggag
     1261 atcaaatgcc aggtgatgct ctgcaatacc tatcaccttc acctgcgccc gggcgacgag
     1321 ctggtgcggg atatgggcgg cctgcaccgc tttacgcgct gggatgggcc gatcctgact
     1381 gattcaggcg ggtttcaggt cttcagcctc gcctcgctgc gcaaaatccg cgaggagggc
     1441 gtcacgttca actcccatat agacgggcgc aaaattttta tgggccccga ggagagcatg
     1501 cgcatccagg cgaacctggg gtcgaccatc gccatggcgt ttgacgagtg catcaaaaac
     1561 cccgctgagc acgggtatgc caaggcctcc tgcgcccgca cggtgcgctg gctgaagcgc
     1621 tgcaaacagg agatggcgcg cctcaagcgg gaggggcttg cggtcaaccc cgaccagctg
     1681 ctgttcggca tcaaccaggg ctgctgcttt gacgacctgc gcgtggagca gatgaaagag
     1741 atcgcggagc tggacctgga cggctacagc atcggcggtc tggcggtggg ggagcccacg
     1801 gaggagatgt accgggtgat caccgtggtg gagccgcaca tgcccaaaga caagatccgc
     1861 tatctgatgg gggtcggtac ccctggcaat atcctggagt ccatcgcacg cggcgtggat
     1921 ctgttcgact gcgtcatgcc cgcgcgcaac gcccgccacg gccacttcaa cacctggggc
     1981 ggcatcatca acatccgcaa cgctaaatac gagagggacg agtcgcccat tgacccgcag
     2041 tgcgactgcc cggtctgccg caatttcagc cgcagctaca tccggcacct cttcaaggca
     2101 gacgagattt tggggctgcg gctcggggtc atgcacaacc tctacttcta caacacgctg
     2161 ctcgaacgct gccgagaggc gcttgacgcg ggcaattttg aggattttcg caaaaaatac
     2221 agcaaaattt tggacagccg tatttgaaag ggctgaaaaa gcttgcaagg ggcccgaaat
     2281 ttggctataa tgaatattgt attgcaatcg accaaacttt aggaggaaga tttttgatgt
     2341 ttccaatttt tctgaccacc ggcagcgacg ctacgtttgg aattatcggc actatcctgc
     2401 cgtttgccct cgttctggtt gtgatgtatt ttctcatgat ccgcccccag aagaaaaagg
     2461 acaagcagct gcaagacctg cgcaactcac tggagatcgg cgacaacgtc accaccatcg
     2521 gcggcatcgt gggccgtgtc gtggcgatca aagacgagac cattgtgctg gagaccggca
     2581 ccgagcgcaa caaggtccgc ttcaagcgct gggcagtgca ggatgtggag aagctcgtcg
     2641 tcgagtaaac aacataaacg cggatacccc tgaatacact tgtagcagtg tatttggggg
     2701 tgttttttgt atggttaaaa aagctggtgc agtaaaaaag tcggagcggc ccttagcccc
     2761 ggcgctcatc ttttcatttt tcacggcggt ggcgctgacc ctggtgcagt tcgctctggt
     2821 gagctcggta atgtgccggg tggatgtgtc cccaaacctg ctgggcccgc tctccaccat
     2881 catggtgtgc atctccgcgc tggcggcggg cacggcgctc gccttttcca tcggcagcaa
     2941 ggggatgctg ctggggtttg cggagggcgc cgttttttat ctgatcctca tggccgcagc
     3001 attcctccag ggaaatttcg attttaccaa tatggcgttt gtcaagctgg tcgcaatgtg
     3061 cgccgcgggg gctgtgggcg gctatctcgg cgtcggcctg gcggagcgaa aacggcgcaa
     3121 gcactaaggg cttttgcccc nnnnnnnnnn tgtcctgaac taaaaaattc catctcaaaa
     3181 ggctgttttg atggcgtaca gacccgtata aagcgcgatt tctgcgcctg tggtctcgcg
     3241 tctcgcggac ggctctccga gctccgcccc tggcggcctc ggtgcagcgc ccgccgttta
     3301 atctgccagg gcgcccccca ggggggcgct tttatatttt aaaagttccc gcgatccgcc
     3361 gcgccccacg tctgcaaaaa gcataaaccc cgctgtgcgc gaataggctt gtactgcgat
     3421 catcgtaaaa agtacaggct gttggc
//
LOCUS       DBMDYA010000261         3433 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6258_length_3433_cov_1.711285, whole genome shotgun sequence.
ACCESSION   DBMDYA010000261 DBMDYA010000000
VERSION     DBMDYA010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_3433_cov_1.711285"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="ACZPFK_07305"
     CDS             <1..195
                     /locus_tag="ACZPFK_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR1406153.1"
                     /translation="GEHCLLHSVESKFAKPVFIGDVLTVTGVVEEVNEVFGEVTIKGT
                     ITNQDGKKVCRGVIKAGLAK"
     gene            1024..1545
                     /locus_tag="ACZPFK_07310"
     CDS             1024..1545
                     /locus_tag="ACZPFK_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1406154.1"
                     /translation="MPYTYLITGATSSVGTALIRRVWKPGDIVIAQGAGDLALLEELR
                     PLCGDALRCYDVDLTGEDSLSAFLAEIKERFGCPTHIVHLPALRVVNKKFKNFDEQRF
                     LLDFNVQVMSALRICKAFLPGMMKARFGRVLFMLTDYIIGVPPKNTTPISWPKTRCAA
                     SPAALPPRRRPPG"
     gene            1479..1754
                     /locus_tag="ACZPFK_07315"
     CDS             1479..1754
                     /locus_tag="ACZPFK_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1406155.1"
                     /translation="MAKNALRGLARSLATEAAPAGVTVNCVAPYMMETRFLTDTPDLI
                     VEASAAANPMGRNARVEDVVPAMAFLLSDEAGFISGAELPVTGGARY"
     gene            1751..2746
                     /locus_tag="ACZPFK_07320"
     CDS             1751..2746
                     /locus_tag="ACZPFK_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406156.1"
                     /translation="MSGYSFRRAVSSDRAAIVQFMNLHWGSRHPLINLPDYFTYYYQN
                     GDPTAELLNFALCLEDEKIAAVCGFIPSSQNGREIWISIWCADKKAKGSGLELMAQMP
                     ALTGAKRMSCNNIRPNTIPFYEFLGYTGARMGHFYRLGRRGEYRVARIADRAILPVSG
                     DGVLRRFKTFEDLKKHFVPPMTAHPYKDLWYLERRYFHYPRQDYLVYGGFAERGCPLL
                     FCLRQVPVNGTCVLRLVDLVGDFALLPRFGRALDGLLAEMDAEYMDCYCWGIPAPTMA
                     AAGLCERNENSVNIIPHYLTPPLIQNVEYYLFTSDPQGFVMFRADGDQDRPNIEC"
     repeat_region   2855..3420
                     /inference="COORDINATES: alignment:CRISPRCasFinder:4.3.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2855..2887
                     /rpt_unit_seq="gtttcaatccacgccgctcttgcgagcggcgac"
BASE COUNT          682 a         1017 c         1007 g          727 t
ORIGIN      
        1 ggagagcact gtttgctgca cagcgtggag agcaagtttg caaaaccggt cttcatcggg
       61 gacgtcctca ccgtcaccgg cgtggtggaa gaggtgaacg aggttttcgg cgaggtgacc
      121 atcaagggca cgatcaccaa tcaggacggc aaaaaggtct gccggggcgt catcaaggcc
      181 ggtctggcaa aatagtgctc aaaaattcgg cagagccgcg ccgccggggc cggagcggct
      241 cggaacacat cgaggcccgg ctgggcgggg cgcgtcccta cgatgcggcc tgaaagaaca
      301 gcagggaggg gcaataaatc tcgctgtccg aaaggttccg tccgcttttg ccgccgtccg
      361 cgcgagggcg cgggttgaaa tagtccctgg gaagtctgtg cgccacggtt cagagccgct
      421 gtcccaagag tcggtgcgga ctttgcaaag cgccgctttg gcggcaatcc gtcgccgttt
      481 gcaaaaagcg aggatcggaa cgggggcggt gccgcaaaac accggctcgg tcagaattgc
      541 cgctcttgca cacagcgtga gttgaaatga agtggaggtc gtgggctggg gcagcgacaa
      601 agaaaggccg ccgctcgcga gggcggcgcg gattgaaaca tcctcaaccc catcgggtat
      661 tttatggact acgtcgccgc tcgcaagagt ggcgtggatt ggaactcctc cacggccgtc
      721 tggatgtcct cgttgcgcag gtcgccgctc gtaagagcgg cgtgaattga aaccggttat
      781 agggtttggt gtcatcttgc ccaaaaggtc gccgctcgcg agaacggcgt ggattgcagc
      841 ttcgatctcc gcaaaaagct ccgcctgccg caggccgcca ttcggaagag gacgacgatc
      901 gaaatcggca aatgctccgc catctgcacg ccaaagacgc ctccgggccg gatgggcggc
      961 tccgaccgaa tgcgcttcgg tcttctgttc gttcactttt caacaagcat agggggaaga
     1021 ttcatgcctt atacctatct tatcaccggc gccaccagca gcgtcggcac cgcgctgatc
     1081 cgcagggttt ggaagcccgg tgacatcgtg atcgcccagg gcgccgggga ccttgcactt
     1141 ttggaagaat tgcggccgct ctgcggcgat gccctgcgct gctatgacgt ggacctgacg
     1201 ggagaggatt ctctgtccgc attcctggcc gaaataaaag agcgtttcgg ctgccccacc
     1261 catatcgtcc atctgccggc actgcgggtg gtgaacaaaa aattcaaaaa ttttgacgaa
     1321 cagcgttttc tgctggattt caatgtgcag gtcatgagcg cgctgcgcat ctgcaaggca
     1381 tttttgcccg gcatgatgaa agccaggttc ggccgcgtcc tctttatgct cactgattac
     1441 atcatcggtg tgccgcccaa gaacaccacg cctatatcgt ggccaaaaac gcgctgcgcg
     1501 gcctcgcccg cagccttgcc accgaggcgg cgcccgccgg ggtgaccgtc aactgcgtgg
     1561 ccccttatat gatggagacc cgttttctca ccgacacgcc ggacctgatc gtggaggcct
     1621 cggccgccgc caaccccatg ggccgcaacg cccgggtgga ggacgtggtg cccgccatgg
     1681 cgtttcttct ctccgacgag gcgggcttta tcagcggcgc agagctgccg gtgacggggg
     1741 gcgcgcgcta ttgagcgggt acagcttccg ccgtgccgtc tcctccgacc gcgcggccat
     1801 cgtccaattt atgaatctcc actggggctc ccggcacccg ctgatcaacc tgccggacta
     1861 tttcacctat tactatcaaa acggcgaccc aacggcggag cttctcaact ttgctctttg
     1921 tctggaagac gaaaaaattg ctgcggtctg cggttttatc ccctcttctc aaaacgggcg
     1981 ggagatctgg atttccatct ggtgcgcgga taaaaaggcc aagggcagcg gtctggagct
     2041 gatggcgcaa atgcccgccc tcaccggtgc aaaacggatg agctgcaaca atatccggcc
     2101 caacaccatc cccttttacg aatttttggg ctataccggc gcgcggatgg ggcattttta
     2161 tcgtctgggc cgccgtggcg agtaccgagt cgcgcgcatt gccgacagag ccatcctgcc
     2221 tgtctcaggt gacggcgttt tgagacgctt taagaccttt gaggacctga aaaagcactt
     2281 tgtcccgccc atgactgcac atccctataa agatctctgg tatcttgagc gccgctattt
     2341 tcattatccc cggcaggact atctggtcta cggcggtttt gccgagaggg ggtgtccgct
     2401 tttgttctgt ctgcgccagg ttcccgtcaa cggcacctgc gtgctgcggc tggtggacct
     2461 agtaggcgat ttcgcccttt tgccccgttt tggcagagcg ctggacggcc tgctcgcgga
     2521 gatggacgcc gaatatatgg actgttattg ctggggcatt cccgccccga caatggctgc
     2581 cgcaggtctg tgcgagcgca acgaaaacag cgtgaatatc attcctcatt atctcacccc
     2641 gcccttgatt cagaatgtgg aatattattt attcaccagt gacccgcaag gatttgtgat
     2701 gttccgcgcc gacggagatc aggaccgccc caacatcgaa tgttgacacg ccctgcctta
     2761 aaagtgaaaa aagtgagcaa aaaagagcag tctttctaag gagaccgctc ttttttgtct
     2821 ggagggctac ggaaacgaca atccgggcgc atttcaatcc atccacgccg ctcttgcgag
     2881 cggcgacgac ctcgatgcgg tcagtggtat attcgtcagt gtttcaatct acgccgctct
     2941 tgtgagcggc gaccttacca cgcaaagcaa gagcaagagg ctttaaagtt tcaatccacg
     3001 ccgctcttgc gagcggcgac ctgccacatg gccatgagct ccggcaggtt atagtttcaa
     3061 tccacgccgc tcttgcgagc ggcgacgcgc aacaacattg tctttgttgc cgaaaacctg
     3121 tttcaatcca cgccgctctt gcgagcggcg accaaaaaga cgccgaggcc aaggtcgcgg
     3181 cgctggtttc aatccacgcc gctcttgcga gcggcgacct tcctgtgttt ttgcattcaa
     3241 accctcggtc tggtttcaat ccacgccgct cttgcgagcg gcgaccgctg tttccccttg
     3301 aacacggtat tatcctggat gtttcaatcc acgccgctct tgcgagcggc gacccgcgat
     3361 gcggcggtcc gcggaggctg ccacctggtt tcaatccacg ccgctcttgc gagcggcgac
     3421 gtgacccggt atg
//
LOCUS       DBMDYA010000262         3430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6263_length_3430_cov_1.467708, whole genome shotgun sequence.
ACCESSION   DBMDYA010000262 DBMDYA010000000
VERSION     DBMDYA010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_3430_cov_1.467708"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="ACZPFK_07325"
     CDS             complement(<1..1857)
                     /locus_tag="ACZPFK_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1406157.1"
                     /translation="MKLLAIDGNSIVNRAFYGIKLLTTKDGRYTNGLVGFMNILLKLL
                     ADEKPDGVAVAFDLPKPTFRHKRYDNYKGTRKGMPEELAQQMPLLKELLTDLGYQIVT
                     AEGFEADDILGTLAADCAADGDECVIATGDRDSLQLVAPATRVLLASTQMGRSVTVNM
                     DEAAVREKYGVEPKQLIEVKALMGDASDNIPGVAGVGEKTALALIAEFGSLAGVYEHL
                     DSPSIKPGVRAKLERDREQAEMSRFLAEIVTDAPVPHDVADYRPGPGNPAAAARLLAS
                     LEMYGLLDKLGLEQSAAAAAAPPQPDAPAPERVKAQPLPEGLKGPVAVAAAADGYSIV
                     RGGALYAAGADDAAFLALLEDEKTEKRVFDAKPLYTLMLSHGRRAAGIVFDGKLAAYL
                     LNPSASGYDPVRLAAEYGVRPAFACDDDAAGVLEPLFDVLAAACEAQGMTRLLREIEL
                     PLSEVLSSMEVTGILVDRQGIEQFGVELKQVLEAELAEIYEQVGYEFNLNSTKQLGDA
                     LFGKLGLPAKKKTKSGWSTNAETLESLRGMHPVVDHILLYRTYQKLNSTYVEGLLKVI
                     GADGRIHSAFSQTETRTGRISSSEPNLQNIPVRTELGSRLRKYFVAPQGRVLL"
     gene            2105..3073
                     /gene="dusB"
                     /locus_tag="ACZPFK_07330"
     CDS             2105..3073
                     /gene="dusB"
                     /locus_tag="ACZPFK_07330"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1406158.1"
                     /translation="MDFKTLFIGPGAAFAPMAGLSDASARRLMASYGAAYTVSEMVSA
                     KALCYGDKKTAALLRGGGGEAPFGIQLFGSEPQIMAEGAKLALRYRPDFIDINMGCPA
                     PKITGGGAGSALMRDPALCGRIVEAVVKAVDLPVTVKMRKGWDEGAVTCVEVARRCEA
                     AGAAAIAVHGRTREQMYTPPIDPGCIAAVKAAVSVPVIGNGDVTSAAAALALLRQTGC
                     DAVMIGRGALGSPWLFAEVKAALAGEPLPPRPGLRQRMAVMCAQLYSMCEEKGEEAAM
                     RQARTQAPYYMKGLRGAAQLRRAACGLTVYRDAEELARLALQLNDG"
     gene            complement(3060..>3430)
                     /locus_tag="ACZPFK_07335"
     CDS             complement(3060..>3430)
                     /locus_tag="ACZPFK_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024319.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HIR1406159.1"
                     /translation="YNNEELKTAYKALEREAEERLAALAPPEVRDGLRGALCGDGLTE
                     REKRILKAADKLSALIKCVEERQGGNLEFESAARAQREYLAAMGLPEADYFMERMLPA
                     YTRTLDELTARESLPDITRR"
BASE COUNT          511 a         1168 c         1162 g          589 t
ORIGIN      
        1 cagcagcacg cggccctgcg gcgcgacgaa gtatttgcgc aggcggctgc ccagctcggt
       61 gcgcaccggg atgttctgca aattcggctc gctggacgag atgcggccgg tgcgggtctc
      121 ggtctggctg aacgccgagt ggatgcgccc gtccgcgccg atgaccttca gcagcccctc
      181 cacataggtg gaattgagct tttgataggt gcggtagagc aggatatggt ccaccaccgg
      241 gtgcatgccc cgcagggatt ccagcgtctc ggcgttggtg gaccagccgc tctttgtctt
      301 tttcttggcg ggcaggccca gcttgccgaa cagcgcgtcg cccagctgct ttgtggagtt
      361 gagattgaac tcgtacccca cctgctcgta gatctcggcc agctcggcct ccagcacctg
      421 tttcagctct acaccgaact gctcgatgcc ctgccggtcc accaaaatgc cggtgacctc
      481 catcgaggag agcacctcgg agaggggcaa ctcgatctct ctcagcagcc gggtcatgcc
      541 ctgcgcctca caagccgccg ccaggacgtc gaacagcggc tccagcacac ccgcagcgtc
      601 gtcgtcacag gcaaaggcgg gccggacgcc gtattcggct gccagccgca ccgggtcgta
      661 gccgctggcc gaggggttga gcagataggc ggccagcttg ccgtcgaaga caatccccgc
      721 ggcgcgcctg ccgtgggaga gcattaacgt gtagagcggc tttgcgtcaa agacccgctt
      781 ttccgtcttt tcatcctcca gcagcgccag aaaggccgca tcgtcggccc ccgcggcata
      841 cagcgcgccg ccgcggacaa tactgtagcc gtccgccgct gcggcgacgg ccacggggcc
      901 tttcagcccc tcgggcagag gctgcgcctt gactcgctcc ggcgccgggg catccggctg
      961 cggcggcgcg gcggcagcgg ccgcgctctg ctccagcccc agcttgtcca gcaggccgta
     1021 catctccagc gaggccagca gccgcgcggc ggcggccggg ttgccggggc cgggccggta
     1081 gtcggccacg tcgtggggca ccggggcgtc ggtgacgatc tcggccaaaa aacggctcat
     1141 ctcggcctgt tccctgtccc gctccagctt ggcccgcacc cccggcttga tggaggggct
     1201 gtccagatgc tcgtagaccc cggccagcga gccgaactcg gcgatcaggg caagggcagt
     1261 cttttcgccc accccggcca cgccggggat gttgtcgctg gcatcgccca tcagggcctt
     1321 gacctcgatg agctgttttg gctcgacgcc gtatttctcc cgcaccgcgg cctcgtccat
     1381 gttgaccgtg acgctgcggc ccatctgggt ggaggccagc agcacccggg tggcgggcgc
     1441 caccagctgc agcgaatccc ggtcgccggt ggcgatgacg cactcgtccc cgtcggccgc
     1501 gcagtcggcg gccagggtgc ccaaaatgtc gtcggcctca aagccctccg ccgtgacgat
     1561 ctggtagccc aggtcggtca gcagctcttt gagcagcggc atctgctggg ccagctcctc
     1621 cggcatgccc ttgcgggtgc ccttgtagtt gtcgtaccgc ttgtggcgaa aggtgggctt
     1681 gggcaggtcg aacgccaccg ccacgccgtc cggcttttca tcggccagca gcttgagcag
     1741 aatgttcata aagcccacca ggccgttggt gtaacgcccg tccttggtgg tgagcagttt
     1801 gatgccgtaa aaggcgcggt tgacgatgct gttgccgtcg atcgcgagaa gtttcaatgg
     1861 gctgcgcctc cctgtctgtg caatttttgt gggtctgcaa gctttgtgca gcgcgccgcg
     1921 ccgccgcccg cgcacgggcg tacaccgccg cggggcgcgc gcttttcagc gaaacatatc
     1981 atgggtcatt ataccacagg acgaaatttt atggtacaat gatttcgtcg ccccgccccg
     2041 ccgcgtccct ctgtctagta tgcggcggcg gcgggcgcaa aaatcaagct cagggagaac
     2101 ttctttggat tttaaaactc ttttcatcgg cccgggagcg gccttcgcgc cgatggcggg
     2161 cctctcggac gccagcgccc ggcggctgat ggcgtcctac ggcgcggcgt acaccgtcag
     2221 cgagatggtc agcgccaagg cgctgtgcta tggcgacaaa aagacggccg cgctgctgcg
     2281 cggcggcggc ggcgaagccc cgttcggcat tcagctcttc ggcagcgagc cgcagatcat
     2341 ggccgagggc gcaaagttgg cgctgcggta tcgccccgac ttcatcgaca tcaacatggg
     2401 ctgccccgcc cccaagatca ccggcggcgg ggcgggcagc gcgctgatgc gcgacccggc
     2461 cctctgcggg cgcatcgtgg aggccgtggt aaaagccgtg gacctgccgg tcacggtgaa
     2521 gatgcgcaag ggctgggacg agggagccgt cacctgcgtg gaggtggcca ggcgctgcga
     2581 ggctgcgggg gccgcggcca tcgccgtgca cgggcgcacc cgcgagcaga tgtacacccc
     2641 gcccatcgac ccgggctgca tcgcggcggt aaaggccgcc gtctcggtgc cggtgatcgg
     2701 caacggcgac gtgaccagcg cggcggcggc cctggcgctg ctgcggcaga ccggctgcga
     2761 cgcagtgatg atcgggcgcg gcgcgctggg cagcccctgg ctgttcgccg aggtgaaagc
     2821 cgcccttgcc ggcgagccgc tgccgccccg gcccggcctg cgccagcgca tggcggtgat
     2881 gtgcgcccag ctttacagta tgtgcgagga aaagggcgag gaggccgcca tgcggcaggc
     2941 ccgcacccag gccccctatt atatgaaagg gctgcggggc gcggcgcagc tgcgccgcgc
     3001 ggcctgcggg ctcaccgtct accgggacgc cgaggagctg gcgcgtttgg cgctgcagct
     3061 caacgacggg tgatgtcggg caggctttct ctcgcggtca gctcgtccag cgtgcgggta
     3121 taggcgggca gcatccgctc cataaaatag tcggcctcgg gcaggcccat ggcggcgaga
     3181 tactcccgct gggcccgggc ggcgctctca aattccaggt tgccgccctg ccgctcctcc
     3241 acacatttga tgagggccga cagcttgtcc gccgccttga ggatgcgctt ttcccgctcg
     3301 gtcagcccgt cgccgcagag cgcgccccgc aggccgtccc gcacctccgg gggcgcgagg
     3361 gcggccagcc gctcctcggc ctcccgctcc agcgccttgt aggcggtttt cagctcctcg
     3421 ttgttgtact
//
LOCUS       DBMDYA010000263         3426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6271_length_3426_cov_1.331128, whole genome shotgun sequence.
ACCESSION   DBMDYA010000263 DBMDYA010000000
VERSION     DBMDYA010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_3426_cov_1.331128"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="ACZPFK_07340"
     CDS             complement(<1..302)
                     /locus_tag="ACZPFK_07340"
                     /inference="COORDINATES: protein motif:HMM:NF048942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrrC family ATP-dependent DNA helicase"
                     /protein_id="HIR1406160.1"
                     /translation="MEHLSGSVEHITFANADTGFAVVELDADGELVTAVGTMPGVAEG
                     EEVELEGEFVTHPSFGPQFKVGSFTCRLPQDTAAVLRYLASGVLPGIGPISARRIV"
     gene            complement(358..693)
                     /locus_tag="ACZPFK_07345"
     CDS             complement(358..693)
                     /locus_tag="ACZPFK_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406161.1"
                     /translation="MNSILVILITGMAVLGAYYLAELFSEGMEKPKNDEAVLVLPGPV
                     TPAQVLEITAMVRQKLPRCQVIAGLGDRVPDAPLPAAGLRGVDFVACRDLEAAVMREL
                     HLQSPGEDL"
     gene            1098..1769
                     /locus_tag="ACZPFK_07350"
     CDS             1098..1769
                     /locus_tag="ACZPFK_07350"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1406162.1"
                     /translation="MTNYQKELEKILAHLQPQGARPRLLLHCCCAPCSSYVLEYLSRY
                     FSITADFYNPNIAPREEHDHRAAELRRLIGEMPLSSPVLFEAGPYEPQLFAEIARGLE
                     DCPEGGERCYRCYRLRLEQAARRAGEGGFDWFTTTLTISPLKNAARLNEIGAELAERY
                     GVRFLPSDFKKREGYKRSIELSRKYGLYRQDYCGCAFSRAAREKELAARSGENGQALR
                     EKPGE"
     gene            2213..3052
                     /locus_tag="ACZPFK_07355"
     CDS             2213..3052
                     /locus_tag="ACZPFK_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1406163.1"
                     /translation="MDRLVTESLLDRFARELLQQEKSPSTVAKYLRDVRAFQRFAAGG
                     RVDKDLVLRYKRQLQQGYATASANSMLVALGCFFHFLGWEDCRVRLFKCQRRVFRDRA
                     RELSREEYLRLVAAARGRGDARLALLLQALCSTGIRVSEHRFITAEAARTGRARVCNK
                     GKERLVLLPRELCRALRDYCRAQGIRSGAVFVTRGGNPLDRSNIWAMMKALCRAAGVE
                     PGKVFPHNLRHLFAVSYYALDKDVVRLADLLGHASIDTTRIYIATSGDEQARRLSRLR
                     LIA"
BASE COUNT          665 a         1086 c          981 g          694 t
ORIGIN      
        1 tccacgatgc gccgcgcgga gatggggccg atgccgggca gcacgccgct ggcgagatag
       61 cgcagcaccg ccgccgtgtc ctgcggcaaa cggcaggtga aagagccgac cttgaactgg
      121 gggccgaagc tggggtgggt gacgaactcg ccctccaact cgacctcctc gccctcggcc
      181 accccgggca tggtgcccac ggcggtcacc aactcgccgt ccgcgtccag ttccaccacg
      241 gcaaacccgg tgtcggcgtt ggcaaaggtg atgtgctcca ccgagccgga cagatgctcc
      301 atgcgcccgc cccctttcca tccttgtctt ttgcggcccc gcggccatac tctgctatta
      361 taaatcctcg cccgggcttt gtaaatgcag ttcccgcatc accgcagcct ccagatcgcg
      421 gcaggcgaca aagtcgacgc cgcgcaggcc cgcggccggc agaggcgcgt cgggcaccct
      481 gtcccccagc cctgcgatca cctggcagcg gggcagcttt tgccgcacca tggcggtgat
      541 ctccaacacc tgggcgggcg tcaccgggcc cggcagcacc agcaccgcct cgtcgttttt
      601 tggcttttcc atcccctcgg aaaacagttc cgccagataa taggccccca gcaccgccat
      661 gccggtgatc aggatcacaa gtatactgtt catcaaacca gccccctccc gtccgcctcg
      721 cagcggccgt ttttttatct gggccatctt atgcgcaggg ggctgatgct gtgaaaaaaa
      781 ggtttttgtt tgacttttca gccttatttc ttatacttat cttgtacttg gctgaaaaat
      841 agccacaggc cattttttcg ggccttcctt tcggttgccg cgactgcggc gagaaacggg
      901 gccattaaga taagtttttt agtgcgcagc aataaaaaat agcctgcggc tattttttac
      961 gcgcatccgc ccactcccgt gggcgggcgc tcaagatttt aaggccgtgg gccgggacgg
     1021 cccacatggc catttttata gctttgcgtt gcaaagctat aaaaaattgc aacaaatttt
     1081 gctttgggag gcgcaccgtg accaattacc aaaaagagct ggaaaaaatc ctcgcccacc
     1141 tgcagccgca gggcgcccgc ccccggctgc tgctgcactg ctgctgtgcg ccctgcagca
     1201 gctatgtact ggaatatctg agccggtatt tttccatcac cgcggacttt tataacccca
     1261 atatcgcccc gcgggaagag catgaccacc gtgccgccga gctgcgccgc ctcattggcg
     1321 agatgccgct ctcctcacct gtgctttttg aggcgggccc ctatgagccc cagctgtttg
     1381 cggagatcgc gcgcggattg gaggactgcc ccgagggcgg cgagcgctgc tatcgctgct
     1441 atcgcctgcg gctggaacag gcggcccggc gcgcagggga gggcggtttc gactggttca
     1501 ccaccaccct caccatcagc ccgctcaaaa acgccgcccg cctcaatgag atcggggcgg
     1561 aactggccga gcgctacggc gtgcgctttc tcccctcaga cttcaaaaaa cgcgagggct
     1621 ataagcgcag catcgagctt tcgaggaaat acggccttta ccggcaggac tactgcggct
     1681 gcgcgttcag ccgggcggcg cgggaaaagg agcttgcggc gcgatcgggt gaaaatggtc
     1741 aagccttgcg ggaaaagccg ggcgaatagg ccctccgcct ccggcgctct gccgcacaaa
     1801 gcgcagagca gtgcgtctct ctttaggaca tgcccggcgc agggcctgcc tccatttcgc
     1861 tgttcctgcc gcaacgcgcc cttgtcgggc gcgttttttc tatcgcaaaa atgaccggtt
     1921 ctctctgcgg atgatgccgg caatcgcaga ggtcggcgcg atgcagcaaa aaacagctgt
     1981 gcgggccgcg catttccccc gttgtccccc ggcgcagagc gagcggacgg ccgccccctg
     2041 cgcgcccttt tcaaccagcc ttgtccaaga gactgatccg gcaaaggcct gtttttttca
     2101 attttaacaa tattttaacg tctcttgcct ccatcttcct ttttctgcct tttaacctcc
     2161 cacaaaaatg gcaatacttt taattatatt gcctttttga ggaggagtaa aaatggaccg
     2221 tctcgtcacc gaatctctgc tcgaccgctt tgcccgggag ctgctgcaac aggaaaaaag
     2281 cccctccacc gtcgccaaat acctgcgcga tgtgcgggct tttcagcggt ttgccgccgg
     2341 cggcagggtg gacaaggacc tggtgctgcg ctacaagcgg cagctgcagc agggctatgc
     2401 caccgccagc gccaactcga tgctggtggc gctgggctgt ttttttcact ttctcggctg
     2461 ggaggactgc cgggtgcgcc tgttcaaatg ccagcgccgc gtgttccgcg accgggcaag
     2521 ggagttgagc cgggaggaat acctgcgcct ggtcgcggcc gcccgcgggc gcggcgacgc
     2581 gcgcctggcc ctgctgctgc aagccctgtg cagcacgggc atccgggtga gcgagcatcg
     2641 cttcatcacc gctgaagccg cgcgcaccgg gcgcgcaaga gtgtgcaaca agggcaagga
     2701 gcgtctggtg ctgttgccgc gggagctgtg ccgcgccctg cgggactatt gccgggcgca
     2761 gggcatccgc agcggcgccg tatttgtgac ccggggcggc aacccgctgg accgcagcaa
     2821 catctgggcc atgatgaaag ccctgtgccg cgcggccggg gtggagccgg gaaaggtctt
     2881 tccccacaac ctgcgccatc tgtttgccgt gagctattac gcactggaca aggatgtggt
     2941 acggctggcc gatctgctgg gccacgccag catcgacacc acccgcatct acatcgccac
     3001 cagcggcgac gagcaagccc gccgtctctc gcgtctgcgg ctgatcgcct gacaaaaaaa
     3061 ttcaccacat aatacggatt atgtggtgtt ttgcatcttg aaaaaagaag aaacaaagcg
     3121 atgagagccg gagtttctct cacggagaaa aaacagcgac gcaccggcac ggcaaagcag
     3181 accctccaaa aagggcctgt ggctttcagc tctcgaggcg cgtccaaagc ggcgacagaa
     3241 atcgcgcttc ttcttgattt tttacagttc atagtgcata ataatatgta attataattc
     3301 cattctaaag aaatagtctc ctgttccacc acataatccg tattctgtcg taaggatggt
     3361 gaccaccaat gtcttgcgca tccccctgcc cggacgccgg cagcgaccgc ttgttggacg
     3421 atctcg
//
LOCUS       DBMDYA010000264         3424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6274_length_3424_cov_1.610894, whole genome shotgun sequence.
ACCESSION   DBMDYA010000264 DBMDYA010000000
VERSION     DBMDYA010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6274_length_3424_cov_1.610894"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="ACZPFK_07360"
     CDS             complement(<1..812)
                     /locus_tag="ACZPFK_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR1406164.1"
                     /translation="MSAVRLHPFHFLKYIKYGLILCVIPILRAMLAFDIAAAWLAFTQ
                     DLLILLACGGILATLWQASCAGLADGVLTVRQGVLLRRPALYRACMVSAVEVCRPLWY
                     RVFGAARLKIYFKAGSGQRRVELVLPGRRARQLARALVPATAAANFFEPVGMDRLIFV
                     LLSANILATSAFSVLAVRRIAKLVGQDLGALALGGITQVEHLLAAFLPAGLSLLAAVL
                     LFFLALSLLASLLRTYRFTATRSGGVLLCRGGLLTHTERRVLVSAITASTLR"
     gene            complement(809..1228)
                     /locus_tag="ACZPFK_07365"
     CDS             complement(809..1228)
                     /locus_tag="ACZPFK_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR1406165.1"
                     /translation="MNYTVTRKGALLPALWLTAVSAAAGLLVWLFFSLAAGLAAFFLL
                     AALSFALPFLHRLHYQVRVAPRELVADRGFFIRTLRRLPLRCVTGVICLSTPLGRLLD
                     ASVVAVYTSGTTLLLVGLPLADAEQLRVALTVGGGPA"
     gene            complement(1259..2689)
                     /locus_tag="ACZPFK_07370"
     CDS             complement(1259..2689)
                     /locus_tag="ACZPFK_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR1406166.1"
                     /translation="MKINKQFLTALLVFLVLGVCTLLLFFLLPSAAHGAAAEPVTLSD
                     RAQNAFASAEVTNAGGSYTVTAEDGVFACDLLVGLPVSQAAFEQLAQQCTSLTAEGPV
                     APPSGSGTVPDYGFDHPLAQVKAAYSDGGGIFIEVGAQIAGTDQYYIRVDHSASVYRI
                     GKEAIRCLLADISAYLDLSLTPDGADGDTLPSRISVTTGAQSLQLEKLPLPETDGAGF
                     SYRYRLAGDRAAYVDPDAYETYFGELGELEADGVVTMRPTSVDLKEYGLADEEACDSI
                     SFTMLGESVRLRLGNRSGDFYYVLREGVPAIYRLSRLAVTWEDADEYALMSRYLMAPA
                     LDKVQEILIEGGGVRYHFTLQNGSASLDGTPLTASTFDSFYALLCSLRAEYKLGAPAQ
                     NIPPDLTVTFVYTDRAAPTASPAPEDVEASPYRTDVIRFIPYGPKRHAIEINGAALYA
                     VRSTYATKVLGVLATLQDGQQVSASW"
     gene            complement(2915..>3424)
                     /locus_tag="ACZPFK_07375"
     CDS             complement(2915..>3424)
                     /locus_tag="ACZPFK_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1406167.1"
                     /translation="QLPILELAKIGNCQALIGVTGRDESNLIACQLAKKVFGVKKTVA
                     RVNNPKNARVLKQLGVDIAVSSTDNIAHLIEREVETDAIHHLLSIAGGDAALTEVLLP
                     DDFKYAGRTLGELDIPHDVVIVFLTRGQELIIPRGNTTLQGGDKVVCVARNDAFHDLA
                     KKWDLQGHQ"
BASE COUNT          672 a         1085 c         1165 g          502 t
ORIGIN      
        1 atgcgcaggg tactggcggt gatggcactc accagcactc tgcgctcggt gtgggtcagc
       61 agcccgcccc gacagagcag cactccaccc gagcgggtgg cagtgaagcg ataggtgcgc
      121 agcaggctgg cgagcagcga gagggccaga aaaaacaaca gcaccgccgc cagcagcgag
      181 aggccggcgg gcagaaaggc agccagcaga tgttccacct gggtgatgcc ccccagcgcc
      241 agcgcgccca ggtcctgccc caccagcttg gcgatccgcc gcaccgccag caccgaaaac
      301 gcgctggtgg cgaggatgtt ggccgagagc aggacaaaga tcagccggtc catgcccacc
      361 ggctcaaaaa agtttgcagc ggcggtggcg ggcaccagcg cccgggccag ctgccgggcc
      421 cggcgcccgg gcaaaaccag ctcgacgcgc cgctgcccgc tgcccgcttt aaaatagatt
      481 ttcagccgcg ccgccccgaa aacccggtac cagagcggcc ggcagacctc cacggcgctg
      541 accatacagg cgcggtagag ggcggggcgg cgcagcagga ccccctggcg cacggtgagc
      601 actccgtccg ccagcccggc gcaggacgcc tgccagagag tggccagaat gcctccgcag
      661 gccagcagga tcaacaggtc ctgggtaaag gccagccagg cggccgcaat gtcgaacgcc
      721 agcatcgccc gcaggatggg tattacgcaa agtattaatc cgtacttgat atactttaaa
      781 aagtgaaaag gatgcagccg caccgcgctc atgccgggcc acctcccacc gtcagggcca
      841 cccgcagctg ctcggcgtcc gccagcggaa gccccaccag cagcagcgtg gtgcccgagg
      901 tgtacaccgc caccaccgag gcgtccagca aacgccccag cggggtggaa agacagataa
      961 ccccggtgac gcagcgcagc ggcaggcggc gcagggtgcg gatgaaaaag ccgcggtccg
     1021 ccaccagctc gcgcggggcg acccgcacct ggtagtgcag ccggtgcaaa aagggcagcg
     1081 cgaacgagag ggccgccagc aggaaaaagg ccgcaaggcc cgccgccagc gagaaaaaca
     1141 gccacacgag aagaccggcc gcggcggaaa ccgccgtcag ccagagggcc ggcaacaaag
     1201 cgccctttcg cgtgactgta taattcattt gaaatactcc tttttgtaaa acgctaaatt
     1261 accaggaggc ggagacctgc tgcccgtcct gcagggtggc cagcaccccc agcacctttg
     1321 tggcataggt gctgcgcacc gcgtagaggg cagcgccgtt gatctcgatg gcgtgccgtt
     1381 tggggccata ggggatgaag cggatcacgt cggtgcggta gggggaggcc tccacgtcct
     1441 ccggcgcagg ggaggccgtc ggcgcggcgc gatcggtgta gacaaaggtg acggtgagat
     1501 cgggggggat gttctgcgcc ggggcgccca gcttgtattc cgcccgcagc gagcagagaa
     1561 gcgcataaaa gctgtcaaag gtggaggcgg tcagcggcgt cccgtccaaa gaggcggaac
     1621 cgttttgcag ggtgaaatga tagcggacgc cgccgccctc gatcaggatc tcctgtacct
     1681 tgtccagggc gggagccatc aggtagcggc tcatgagcgc gtattcgtcc gcgtcctccc
     1741 aggtgacggc caaccgcgag aggcggtaga tcgcgggcac gccctcgcgc agcacataat
     1801 aaaaatcacc gctgcggttg cccagccgca gccgcaccga ttcgcccagc atggtgaaag
     1861 agatgctgtc gcaggcctct tcatcggcca ggccgtattc tttcaggtcc accgaggtgg
     1921 ggcgcatcgt gaccacgccg tcggcctcca gctcgcccag ttcgccgaaa taggtctcgt
     1981 aggcgtcggg gtccacataa gcggcgcggt cgccggccag acggtagcgg taagagaacc
     2041 ccgcgccgtc ggtctcgggc agaggcagct tttccagctg aagcgactgc gcgccggtgg
     2101 tgaccgagat gcggctgggc agggtgtccc cgtccgcccc gtccggggtg agggagaggt
     2161 ccagataggc gctgatatcc gccagcaggc agcggatcgc ctccttgccg atgcggtaga
     2221 cagaggcgga gtggtccacg cggatgtagt attggtcggt gccggcgatc tgagcgccca
     2281 cctcgataaa gatgccgccg ccgtccgagt aggctgcctt tacctgggcc agcggatggt
     2341 cgaagccgta gtccggcact gtgccgctgc cggagggggg agccaccggc ccctcggcgg
     2401 tcaggctggt gcactgctgc gccagctgct cgaacgccgc ctgggacacc ggcaggccga
     2461 cgagcaggtc gcaggcaaag acgccgtcct ccgccgtgac ggtatagctg ccgccggcgt
     2521 tggtgacctc tgccgaggca aaggcgttct gcgcccggtc ggagagggtc accggctcgg
     2581 cggcggcgcc gtgcgcggct gaggggagaa gaaaaaagag cagcagggta cagacaccca
     2641 gcaccagaaa gaccagcagg gccgtgagga actgcttgtt gattttcatg agagcctccg
     2701 cagaaaacgg gattccccgc atcacttatc gtacctttta ttatacagaa tatgccgccg
     2761 gaagaaaagg tcacggaacg ggaaaaatcg acccgccctg tcgggccacg gccgggaaaa
     2821 gcggcaaaaa agggccgcgg agcggctgct ccgcggcccg ggccggcgga tggatcgtca
     2881 aaaggggccg tgtcggcccg ccgggagagg ggcgttactg gtggccctgc aggtcccact
     2941 tttttgccag atcgtgaaag gcgtcgttcc gcgccacgca gacgaccttg tccccgccct
     3001 gcagcgtggt attgccgcgg gggatgatca gttcctgccc gcgggtgaga aagacgatga
     3061 ccacatcgtg ggggatgtcc agctcgccca gggtgcggcc cgcgtacttg aaatcgtccg
     3121 gcagcagcac ctcagtgagg gccgcgtcgc ccccggcgat gctgagcaga tgatggatgg
     3181 cgtcggtctc cacctcgcgc tcgatcaggt gggcgatgtt gtcggtgctg ctgaccgcga
     3241 tatccacgcc gagctgcttg agcacccgtg catttttggg gttgttgacc cgggccaccg
     3301 tctttttgac gccgaacacc ttttttgcca gctggcaggc gatcagattg ctctcatccc
     3361 tgccggtgac cccgatcagg gcctggcagt tgccgatctt ggcgagctcc aggatcggca
     3421 actg
//
LOCUS       DBMDYA010000265         3419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6281_length_3419_cov_1.181435, whole genome shotgun sequence.
ACCESSION   DBMDYA010000265 DBMDYA010000000
VERSION     DBMDYA010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_3419_cov_1.181435"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="ACZPFK_07380"
     CDS             complement(<1..143)
                     /locus_tag="ACZPFK_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013308.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR1406168.1"
                     /translation="MLDGKKVTAVVVAAGASRRMGFDKLFYILQGKPVLAHTLDAFQA
                     HPLV"
     gene            complement(158..>343)
                     /locus_tag="ACZPFK_07385"
     CDS             complement(158..>343)
                     /locus_tag="ACZPFK_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406169.1"
                     /translation="RAANVTPEQKNWGILLFFSRWAVAVAVIALSLLIPAVDSVAVIL
                     PLTVPVLAAAVLMGLEK"
     assembly_gap    344..353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(826..1590)
                     /gene="spo0A"
                     /locus_tag="ACZPFK_07390"
     CDS             complement(826..1590)
                     /gene="spo0A"
                     /locus_tag="ACZPFK_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HIR1406170.1"
                     /translation="MEARAKVLMTDSKDGQSRLCAEALEKAGAQVILCEKNGARVLQS
                     VLDEQPDACLLDAFMPGLDAISVKARYEEERPNGKKIMFFAMGSAENDTFQNELMESG
                     IAYYFLKPFDVNAMARMVNKAAGGARPQLPSLGDDEFKVTEILHQIGVPAHIKGYQFL
                     RCAILMNVEDPTLINSVTKRLYPEIAKRNDTTASRVERAIRHAIEVAWDRGDVDVLNS
                     YFGYTIHNLRGKPTNSEFIAMISDRMRLEKKVGRVG"
     gene            complement(1712..3004)
                     /gene="spoIVB"
                     /locus_tag="ACZPFK_07395"
     CDS             complement(1712..3004)
                     /gene="spoIVB"
                     /locus_tag="ACZPFK_07395"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HIR1406171.1"
                     /translation="MSKTIKKTAAFLLLAALVLLAALRVTVSSMLPDTFYLAGEEQLR
                     IASLPFVSARQPQGEVAAASTEPGSSYNVDLRLFGLLPLKTVRAEMVQRRRVEVCGTG
                     FGIKMFSSGVMVVGFSDILSTGGYENPAKDAGLSLGDYVITVDGTKVKTNEQMGELVG
                     KSNGAPLEVVYTHDGERRTTSLQPVKDRATGGWRAGMWVRDSSAGIGTLTFIDTNASV
                     FAGLGHSITDVDTGENVSLGSGEIVPVDISGAVAGTPGLPGELKGSFASFVPMGTIRV
                     NGDTGVYGLIYDTAGKGAMMETAQQQEVVPGPAQILTTIDGEGPKAYDVEIEELSFNA
                     ADPNKNMMLHVTDKKLLAQTGGIVQGMSGSPIIQNGRLVGAVTHVLVRDPTRGFGIFV
                     ENMLKTADQVTAGKNGEAARNGDAKKVAGLSTTYTKKA"
     gene            complement(3193..3399)
                     /locus_tag="ACZPFK_07400"
     CDS             complement(3193..3399)
                     /locus_tag="ACZPFK_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406172.1"
                     /translation="MSLREMHEQQKQSNSQNQNNQQNQSNRQNQNSQQNQNNQQNQNN
                     RQNQNSQQNQNSRQSQNNSQQNNR"
BASE COUNT          587 a         1093 c          969 g          760 t
ORIGIN      
        1 acgaggggat gcgcctgaaa ggcgtccagg gtgtgagcga gcaccggctt gccctgcaat
       61 atatagaaca gcttgtcgaa ccccatccgc cgggaggccc ccgccgccac gacgacggcc
      121 gtcacttttt tcccgtccag caccgcgctt ctccccctta cttttcaagc cccatcagca
      181 ccgcggccgc gagcaccggc accgtgaggg gcaggatgac cgccacgctg tcgacggcgg
      241 ggatcagcag cgagagcgcg atcacagcca ccgccaccgc ccagcgggaa aagaacagca
      301 ggatccccca gtttttttgc tccggcgtca cgtttgcggc gcgnnnnnnn nnnaaacaag
      361 aacaggcaga ggcaggccgc cccaaacccc accgcgaggc ccgtcagcac cggcagccgg
      421 aagctgccga gcagcgcgaa aacgcccccg aacagcgccg tggcggccag agtcaccgcc
      481 ccggccaaaa gcgcggtctt ctttttcatc tgtttcaacc tgatttctct gtgtaaaatg
      541 gtctgtctga atcgcatcat aagacagcat aattatagta aaatcggccg aaaaaatcta
      601 ccgcgcgcgg agaaaaagtt ttgccgccct gctccgtgat cttcaccggg gctcggggcg
      661 gcttttttgc tgataaaaac ggacgtattt tcccccgctc tcagggctct gcccgggccg
      721 ggggatgcac cccgctttac gggccgctgc agagcctgcg cgggcagaca aaaagcgtgc
      781 gccgcagcgc acgcttttga tcgcatccct gttccagccg tgggttcacc ccacccggcc
      841 gacctttttt tccagccgca tgcggtcgga gatcatggcg atgaactccg agttcgtggg
      901 cttgccgcgc aggttgtgga tggtgtagcc gaaatagctg ttgagcacat ccacatcccc
      961 cctgtcccag gccacctcga tggcgtggcg gatggcccgc tccacccggc tggcggtggt
     1021 gtcgttgcgc ttggcgatct cggggtaaag gcgtttggtg accgagttga tcagcgtggg
     1081 gtcctccacg ttcatcagga tcgcgcagcg caaaaactgg tatcccttga tgtgcgcggg
     1141 caccccgatc tggtgcagaa tctcggtgac cttgaactca tcgtcgccca gggacggaag
     1201 ctggggccgc gcgccgcccg ccgccttgtt caccatgcgg gccatggcgt tcacatcgaa
     1261 aggctttaaa aaataatagg cgatgccgct ctccatgagc tcgttttgaa aggtgtcgtt
     1321 ttccgcactg cccattgcaa agaacatgat cttttttcca ttggggcgtt cttcctcata
     1381 ccgcgctttg acgctgatgg cgtcaaggcc gggcataaac gcgtcgagaa gacaggcgtc
     1441 cggctgctcg tccagcaccg attgcagcac gcgcgcgccg tttttctcac acaggatgac
     1501 ctgggcgccg gctttctcca gcgcctccgc gcacaggcgc gactgcccgt cctttgaatc
     1561 ggtcatcaac acttttgccc tggcttccat tcaaattcct ccaaatattc gtatattctt
     1621 tgcaactctc ttcactggca atagattacc atatttttcc atttattgca acagatttcg
     1681 aataggagtt tttgcacttt ccacactact tttaggcttt ctttgtgtaa gttgtcgata
     1741 ggccggcaac tttttttgcg tctccgtttc gggccgcttc cccattcttc ccggcggtga
     1801 cctgatctgc ggttttgagc atgttttcga caaagatgcc aaagcccctg gtggggtccc
     1861 gcaccagcac atgggtgacg gcccccacca ggcgtccgtt ctggatgatc gggctgccgc
     1921 tcatgccctg cacgatgccg ccggtctggg ccagcagctt tttgtcggtg acgtgcagca
     1981 tcatattctt gttggggtcg gcggcgttga acgagagctc ctcgatctcc acatcatagg
     2041 ccttgggtcc ctcgccgtcg atggtggtga gaatctgggc cgggccgggc acgacctcct
     2101 gctgctgcgc cgtctccatc atggcgccct tgcccgcggt gtcgtagatc agcccgtaca
     2161 ccccggtgtc gccgttgacc cggatggtgc ccatgggcac aaagctggca aagctgccct
     2221 tgagctcgcc cggcaggccg ggcgtaccgg cgacggcccc cgagatgtcc accggcacga
     2281 tctcgccgct gcccagggac acgttttcgc cggtgtccac atcggtgatg ctgtgcccca
     2341 gaccggcaaa gaccgaggcg ttggtgtcga tgaacgtcag ggtgccgata cccgccgatg
     2401 aatcccgcac ccacatgccc gcgcgccagc cgccggtggc cctgtctttc accggctgca
     2461 ggctggtggt gcggcgctcg ccgtcgtggg tgtagaccac ctccagcggc gcgccgttgc
     2521 ttttgcccac cagctccccc atctgctcgt tggtctttac cttggtgccg tccaccgtga
     2581 tcacataatc ccccagcgaa aggcccgcgt ccttggccgg gttttcatat ccgccggtgc
     2641 tcaggatgtc gctgaacccc accaccatga ccccgctgga aaacatcttg atgccaaagc
     2701 cggtgccgca gacctccacc cggcgccgct gcaccatctc cgcccgcacg gttttgagcg
     2761 gcaaaaggcc gaacaggcgc aggtccacat tgtaagagct gcccggttcg gtgctggccg
     2821 ccgcgacctc cccctgcggc tggcgggcgc tcacaaaggg cagggaggcg atgcgcagct
     2881 gttcctctcc ggcgagataa aaagtgtccg gcagcatgga tgaaacggtc acccgcaagg
     2941 ctgccagcag caccaacgcc gccagcaaca gaaacgccgc cgtttttttg atcgttttgg
     3001 acaaaaaaac acccccaaat aggataggcc cgacgaaagc gcttctctcg cctgcctgtc
     3061 cgggggtgtc ttttgcgccc cctggccgtt agtatttcct gccggggcgc gaatatttga
     3121 gctgctgtga aaaagaggac ggatgggcgg ctttcccact tttgtgggcc ggccgccctc
     3181 gccgcacaaa ggtcagcggt tgttctgctg gctgttgttc tggctctggc ggctgttctg
     3241 attctgctgg ctgttctggt tctgtcggtt gttttgattc tgctgattgt tctggttctg
     3301 ctggctattc tgattctgcc ggttgctctg attctgctga ttgttctggt tctggctgtt
     3361 gctctgcttc tgctgttcgt gcatttctct caaactcatg ctccatgctc tcctttccg
//
LOCUS       DBMDYA010000266         3415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6288_length_3415_cov_1.249547, whole genome shotgun sequence.
ACCESSION   DBMDYA010000266 DBMDYA010000000
VERSION     DBMDYA010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_3415_cov_1.249547"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="ACZPFK_07405"
     CDS             <1..1123
                     /locus_tag="ACZPFK_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1406173.1"
                     /translation="SRKMKKLVAMVLAMALAFSLAACGGSDGSSAAAGGSSAAAGGSS
                     AGGAAAGSSGKIAVIRQLANSDHTTQFFAGCIAEGEALGYTVDTFTADGDDVKMQDLM
                     EQALQKDYDIWIVSHANEGYQYDLVSRAVEKGVKVVGFDCGGEHVEGVTYTSQDDEAL
                     ASISLDGLIAGAKEKGASEPVKFLEINVLGFIVPFDTRHASIEQYVADGKLEVAQIIS
                     PKTSGGDTYSEIYTAVSTALAQDTNKEIHGIWAASSGYLDGAVAALQDSGRDDVVLSA
                     VDISDTEIKRLVDVPDYVSCAAVDPYVIGMVDVRLAVLKTLGVETPETYALDAVLVTG
                     KDLTAEDTMQTLNKYFADFGSTDAFDTDEIKALREKFAK"
     gene            1196..2713
                     /locus_tag="ACZPFK_07410"
     CDS             1196..2713
                     /locus_tag="ACZPFK_07410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1406174.1"
                     /translation="MSQIAMKDISVEFPGVKALQHVGCDFEGGRVKALVGANGAGKST
                     LMKVLSGVNSNYTGQITFDGRPVEIRSPGDAKKLGIEIVYQEVDTALVPSLSVGENIM
                     TEYLVYGLKKGGVVNWKHLHSEAARVLERLHVPLDTHVLVSSLTLAQKQMVVIARAAL
                     GNCSFLILDEPTAPLSQNETEKLFDLVRQLKAEGVGIIFISHRLDELFEICDSITVLR
                     DGQLIKTFDLDETTTIPSIVELMLGRAYNEAIDRSGREIGGTLLETRGLADREHRVTG
                     VDMTVRRGEIVGLSGLVGAGKTELCKTLFGAFGKQEGELLLNGRPVQASSPAQAVKSG
                     MAFIPEERRKEGILVGEPVVSNLSLATLSKHTRALSVIDRKSESQAAEGKVEDLKIKT
                     PSIRQRVGLLSGGNQQKVTIGKWLDSGADLYIFDEPTKGIDVGAKAEVYRLIVELARQ
                     GKGIIYASSEQSEIMSLTDRVYVMYSGKIQKELVTAETDEAELLFYSTGGTKDEN"
     gene            2703..>3415
                     /locus_tag="ACZPFK_07415"
     CDS             2703..>3415
                     /locus_tag="ACZPFK_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406175.1"
                     /translation="MKTKTNNFQIGRFLVNWGAIVAMVMAFVIFSVTAPQAFFSWANM
                     VTILRSISITTVIALGATFAFSTGVMDLSIGSTATMAAALSVTFIAWYGIPMPIAILL
                     TLIICMAIGGVNAFFVIKTHVPAFLATLAMQFVLDGAELTYSGGALINPKVAPAGRDI
                     TLTAVPDLFNSLGKAPLIILIMLVCIVLVELFQGQTKHGRFLYMVGSNPEAARLSGVK
                     VNLYRALAFMLTAVFAGIGG"
BASE COUNT          663 a         1063 c         1075 g          614 t
ORIGIN      
        1 gagcagaaag atgaaaaaac tagtagcaat ggtactggca atggcattgg cgttcagcct
       61 ggccgcctgc ggcggctcag acggttccag cgcggcggcg gggggcagct ccgcggcggc
      121 gggcggttct tccgccggcg gcgcggcagc cggctcctcc ggcaagatcg cggtcatccg
      181 gcagcttgcc aactccgacc acaccaccca gttctttgcc ggctgcatcg ccgagggcga
      241 ggctttgggc tacacggtgg acaccttcac cgcagacggc gacgacgtga agatgcagga
      301 cctgatggag caggcgctgc aaaaggatta tgacatctgg atcgtctccc acgccaacga
      361 gggctaccag tacgatctgg tctcccgcgc ggtggaaaaa ggcgtcaagg tggtgggctt
      421 tgactgcggc ggcgagcatg tggagggcgt cacctacacc tcgcaggacg acgaagcgtt
      481 ggccagcatc tcgctggacg gcctgatcgc cggcgcaaaa gagaagggcg ccagcgagcc
      541 ggtcaagttc cttgagatca acgtgctggg cttcatcgtg cctttcgaca cccgtcacgc
      601 ctctattgaa cagtatgtgg ccgacggcaa gctggaggtc gcccagatca tctcccccaa
      661 gaccagcggc ggcgacacct acagcgaaat ctacaccgca gtttccaccg ccctggcaca
      721 ggataccaac aaagagatcc atggtatttg ggctgcttcc tccggctatc tggacggcgc
      781 cgtcgccgct ttgcaggatt ccggccgtga cgacgtggtg ctctcagccg tcgatatctc
      841 cgacaccgag atcaagcgcc tggtggacgt gcccgactat gtgagctgcg ccgcggtcga
      901 cccgtatgtg atcggcatgg tggacgtgcg cctggccgtg ctgaagaccc tgggcgtcga
      961 gacccccgag acctatgcgc tggacgccgt tctggtcacc ggcaaagacc tgaccgccga
     1021 agacaccatg cagaccctga acaagtactt tgcggacttc ggctccaccg acgctttcga
     1081 caccgacgag atcaaggcgc tgcgcgaaaa atttgcgaaa taactttttc ggcggatcgg
     1141 gccgacgtcg gtcgttggcc cgatcttgtt gtagaacatg gaaaggagtg tcggaatgag
     1201 ccagatcgcc atgaaagaca tctcggtgga gttccccggc gtcaaggccc tgcagcatgt
     1261 gggctgcgac ttcgagggcg gccgggtaaa ggcgctggtg ggcgccaacg gcgcgggcaa
     1321 gagcaccctg atgaaagtgc tctccggcgt caactcgaac tacaccggcc agatcacctt
     1381 tgacgggcgg ccggtggaga tccgctcccc cggggacgcg aaaaagctgg gcatcgagat
     1441 cgtctatcag gaggtggaca ccgccctggt gcccagcctg tcggtgggcg agaacatcat
     1501 gaccgaatac ctggtatacg gcctgaaaaa gggcggcgtc gtgaactgga agcatctgca
     1561 cagcgaggcg gccagggtgc tggagcggct gcatgtgccg ctggacaccc acgtgctggt
     1621 ctcctcgctg acgctggcac aaaaacagat ggtggtcatc gcccgcgccg cgctggggaa
     1681 ctgcagcttt ctgatcctgg acgaacccac cgcgcccctg agccaaaacg agaccgaaaa
     1741 gctgttcgac ctggtgcgcc agctgaaagc cgagggcgtg ggcatcatct ttatctccca
     1801 ccggctggat gagctgttcg agatctgtga cagcatcacg gtgctgcggg acggccagct
     1861 catcaagacc tttgacctgg acgagaccac caccatcccc tccattgtgg agctgatgct
     1921 gggccgtgcc tacaacgagg ccatcgacag aagcggccgg gagatcggcg ggacgctgct
     1981 ggagacccgc ggcctggccg acagggagca tagggtcacc ggcgtcgaca tgacggtgcg
     2041 ccggggcgag atcgtgggcc tctccggcct ggtgggcgcg ggcaagaccg agctgtgcaa
     2101 gaccctgttc ggcgcttttg gcaaacagga gggcgagctg ctgctgaacg gcaggccggt
     2161 acaagcgtcc tctcccgccc aggcggtgaa aagcgggatg gcttttatcc ccgaggagcg
     2221 ccgcaaggag gggatattgg tgggcgagcc ggttgtttca aacctgtcgc tggccaccct
     2281 gtccaagcat acccgcgcgc tttcggtgat cgaccgcaaa agcgagtccc aggccgccga
     2341 gggcaaggtg gaggatctga agatcaagac cccctccatc cgccagcggg tggggctgct
     2401 ctcgggcggc aaccagcaga aagtgaccat cggcaaatgg ctggactccg gcgccgacct
     2461 ttacatcttc gacgagccca ccaagggcat cgacgtgggg gccaaggcgg aggtctaccg
     2521 cctgatcgtg gagctggccc gccagggcaa gggcatcatc tacgcctcca gcgagcagtc
     2581 ggagatcatg agcctgaccg accgggtcta tgtgatgtac agcggaaaaa ttcagaaaga
     2641 gctcgtgacc gccgagacgg acgaggcgga gctgctgttc tattcaacgg gaggaaccaa
     2701 ggatgaaaac taaaacaaac aattttcaga tcgggcgctt tctggtaaac tggggcgcca
     2761 tcgtggccat ggtgatggcg tttgtgatct tctccgtcac cgcgccccag gcgtttttct
     2821 cctgggccaa catggtgacc atcctgcgct ctatctccat caccaccgtc atcgccctgg
     2881 gcgccacctt tgctttctcc accggcgtga tggatctgtc catcggttct accgccacta
     2941 tggccgcggc tctcagcgtc actttcatcg cctggtatgg catccccatg ccgatcgcca
     3001 tcctgctgac cctgatcatt tgcatggcca tcggcggcgt caacgccttt ttcgtcatca
     3061 agacccacgt gcccgccttt ctcgccaccc tggccatgca atttgtgctg gacggcgccg
     3121 agctgaccta ctccggcggc gcgctcatca accccaaggt ggcccccgca ggccgcgaca
     3181 tcaccctgac cgccgtgccc gacctgttca acagcctggg caaggcgccg ctgatcatcc
     3241 tcatcatgct ggtctgcatc gtgctcgtgg agctgttcca gggccagacc aagcacgggc
     3301 gctttttgta catggtgggc tccaaccccg aggccgcgcg gctgtccggc gtcaaggtaa
     3361 atctgtaccg ggccctggct tttatgctca ctgcggtctt cgcgggcatc ggcgg
//
LOCUS       DBMDYA010000267         3414 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6290_length_3414_cov_1.338968, whole genome shotgun sequence.
ACCESSION   DBMDYA010000267 DBMDYA010000000
VERSION     DBMDYA010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6290_length_3414_cov_1.338968"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="ACZPFK_07420"
     CDS             <1..673
                     /locus_tag="ACZPFK_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406176.1"
                     /translation="IRIPHQPRAGGGGVFACVRGDLAESMRFVRREKPVFLKVIGIVA
                     VFNLLLSSMMVVGVPVLVVKLLGRSDGELGLTQGALALGGLAGGLLAGVLGARLKMAR
                     AHRLLFLCALSVAGMGAALGLGAPAFAGYLIVTLLSFFAMAVSTMFSVEMITVVQAGT
                     PPQLVGKVMACLMTLAMCAQPVGQALYGVLFERFEAAAWAILLAAALAAALLALCSKR
                     VFRRL"
     gene            complement(2041..2994)
                     /locus_tag="ACZPFK_07425"
     CDS             complement(2041..2994)
                     /locus_tag="ACZPFK_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1406177.1"
                     /translation="MNIVLLESLAVSDAKIAGLAAPLIAAGHRFTAYTDGNYDPAVLI
                     ERAKDADILMVASTRLPAEVVAACPRLKLISVAFTGVDHLPMDLCRERGILVSNCAGY
                     SNQSVAELVFGLVLSLLRRIPACNEACRSGGTKAGLVGFELAGKTFGIVGTGAIGLRT
                     AQLAKAFGCELVAYSRSRRPEAEALGIRYLPLEQLMAEADVVSLHVPATAETRQLISA
                     EMIARMKPTALLINTARGPVVDNAALAEALKAGKIAGAGIDVFEMEPPLPADHPLCGA
                     PRAILTPHVAFATAESMVVRAGMVFENVSLYLQGKPRNLMK"
     gene            3199..>3414
                     /locus_tag="ACZPFK_07430"
     CDS             3199..>3414
                     /locus_tag="ACZPFK_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406178.1"
                     /translation="MAETQLAAKPLFSRPALRRLIWPLVGEQFLAVMTGMADTVMVAN
                     AGETAVSAVSLVDSINILMLNIFAALAA"
BASE COUNT          515 a         1017 c         1292 g          590 t
ORIGIN      
        1 catccgcatc ccgcatcagc cgcgcgcggg gggcgggggc gttttcgcct gcgtgcgggg
       61 agatctggcc gagagcatgc gctttgtgcg ccgcgaaaag ccggtctttt tgaaagtgat
      121 cggcatcgtg gccgtcttca acctgctgct cagctcgatg atggtggtgg gggtgccggt
      181 gctggtggtg aagctgctgg gccgcagcga cggcgagctg gggctgaccc agggcgcgct
      241 ggcgctgggc ggcctggcgg gcggcctgct ggcgggggtg ctgggcgcgc ggctgaagat
      301 ggcccgggcg caccggctgc tgtttctctg cgcgctgtcg gtggcgggca tgggggcggc
      361 gctggggctg ggcgcgcccg cctttgccgg ctatctgatc gtcaccctgc tcagcttttt
      421 cgctatggcg gtctccacca tgttcagtgt ggagatgatt acggtggtgc aggcgggcac
      481 cccgccccag ctggtgggca aggtgatggc ctgcctgatg acgctggcca tgtgcgccca
      541 gccggtgggc caggcgcttt acggcgtgct gtttgagcgc tttgaagccg cggcctgggc
      601 aattctgctg gctgcggcgc tggcggcggc gctgctggcc ctgtgctcaa agcgggtctt
      661 tcgccggctg tgagggagag ggcggaggct cttttgcggc gcggagtttt tgcggccgcg
      721 cgctgccctg agcgtggcgc ggaatatgag gcgcgcggcg gcgacgcggc cttgccggcg
      781 gtgcggcgca gggcgcgggc gggcctttgc ttgggaggtg gccgcaagcc cccctcgggc
      841 ggcctttggc agtcggcgcc tcggagcggg gcgacgcgga gcggggcgtc gtgggagtaa
      901 cgggcggagg ggaagccgtg ggcgggcgcg gccccggcaa gacacaagac ctgggcacgt
      961 gacttttgcc tccgcctgcg ggcgcagccg ggagtgagag acacccccgg caaggggctg
     1021 ctccgcgctt gtggacggct ctatcttgtc gcgggaaatg cgcgccgcgt tcggcaagga
     1081 gcggcctcgt gctcagggac agcgctatcc tgtcgccggg agtgcgcgcc gcgactggca
     1141 aggtgcaggc ctcccaactt cactttctgc tgcttttgcg gtttccggcg gagggaggcg
     1201 gtttctgctc cgcgggaacc gcagggggag cagagagagc gcagggggcc gcagagagcc
     1261 gcagagagcc gcagagggag cggggcgcgg gacagggagc cgaggccacg gagagtgggg
     1321 cgcgcggcgg cgacgcggcc tttccggcgg cgcgggagat gcgctgtggc gggcgcggtg
     1381 gggcgcgcgt gaaaaaaggg ccgtggcccc gggcgtcttg aggcggggaa acgttgcttt
     1441 taaacgggcg gttccgacgt tccatatccc tctattaaaa atccgcagat taaaaaggtt
     1501 ggcgatccgc agggatcgtc aacctttttg tagggtacct tttttaaaac cgccgcttca
     1561 gagccgccgc ccgtccccca gggggaaaag ccgcggcgcg ggaaaagctc gcgccgcgcg
     1621 cagatccccc ctctcgggga gaccgccttt ttgcggagac aggccgtcgc cgcggcgttt
     1681 tttattccat cgtccgccca gccgtcataa ccccccgcga cggccgcggg gcggatggga
     1741 cggcgcggcg cgcgcaaagc ccccctctcg ggggaagacc gcctttttgc ggacacgggc
     1801 ctgggacgcg gcgctttttc attggttgga ggaaccagcg gctaagccgg tgaaagtacc
     1861 ctctaaaaaa gctttaggtt ccagcaacaa gagacgcaag ccgcttgata accggccccg
     1921 aggcgaaaga aagctttcgc cagacgagcg accgcccgtt tacgggctgt agggcaagcg
     1981 atacaagtgg aaaattcagt gcgtcttgag aggcttgcgg accggaccgg cccgcgccgc
     2041 ttatttcatc aggttgcggg gcttgccctg caggtagagg gagacgtttt cgaacaccat
     2101 gcccgcgcgc accaccatgc tctcggcggt ggcaaaggcc acgtgggggg tgaggatggc
     2161 gcgcggcgcg ccgcagagcg ggtgatccgc cgggaggggc ggctccatct cgaacacgtc
     2221 gatgcccgcg ccggcgattt tgcccgcttt gagggcctcg gccagggcgg cgttgtccac
     2281 caccgggccg cgtgcggtgt tgatgagcag ggcggtgggc ttcatgcggg cgatcatctc
     2341 ggcgctgatg agctgccggg tctcggcggt ggcgggcacg tgcaggctca ccacgtccgc
     2401 ctcggccatc agctgctcca gcggcagata gcggatgccc agggcctcgg cctccgggcg
     2461 acgcgagcgg ctgtaggcga ccagctcgca gccgaacgcc ttggccagct gggcggtgcg
     2521 caggccgatg gcgccggtgc ccacgatgcc gaacgtcttg cccgccagct caaagcccac
     2581 cagcccggcc ttggtgccgc cgctgcggca ggcctcgttg caggcgggga tgcggcgcag
     2641 cagcgagagc accagcccga agaccagctc ggccacgctc tggttcgagt agcccgcgca
     2701 gttggagacc aggatacccc gctcgcggca caggtccatg ggcaggtggt ccacgccggt
     2761 aaaggccacc gagatcagct tgaggcgggg gcaggcggcc accacctcgg cgggcagcct
     2821 ggtgctggca accatcagga tgtcggcgtc cttggcacgc tcgatgagca cggcggggtc
     2881 gtaattgccg tcggtgtagg cggtgaagcg gtgcccggcg gcgatgagag gggccgccag
     2941 cccggcgatc ttggcgtcgc tgacggcgag gctttccagc agaacgatat tcatacggtt
     3001 gacttccttt ccggtgttgg gcgtttggcg ccggggcgcc cggccttttc cgcttttcat
     3061 tataccaaat cggggcggcg gcgcaaccgg gaaaatttat tctttgcccg gcgcggtcaa
     3121 acgattgact ttgcccgtca aaaggcatag aatttcactg taaaatttgt gtaaaacaga
     3181 cgagaaaaga ggatggagat ggcagaaacg caactggctg caaagccgct gttttcccgc
     3241 ccggcgctgc ggcggctgat ctggccgctg gtgggcgagc agtttttggc ggtgatgacc
     3301 ggcatggccg acacggtgat ggtggccaac gcgggcgaga cggcggtgtc ggcggtgtcg
     3361 ctggtggatt cgatcaacat tttgatgctg aacatctttg cggccctggc ggcg
//
LOCUS       DBMDYA010000268         3408 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6305_length_3408_cov_1.622619, whole genome shotgun sequence.
ACCESSION   DBMDYA010000268 DBMDYA010000000
VERSION     DBMDYA010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_3408_cov_1.622619"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..1039
                     /locus_tag="ACZPFK_07435"
     CDS             20..1039
                     /locus_tag="ACZPFK_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1406179.1"
                     /translation="MKKLLAALLAAAMMAAALAGCGGSDSSSAAGGASGGAASGASQT
                     AAGDPLEFYFIALMTGGAAWSRAEKGFNDACAEFGVNGHYLAPVERNNAVEMAELLDK
                     AIVAKADAVFGVFLSTDMFGPGLLKAREQGAVTGSVQLTLPEEYIDFQIGTDQQQIGV
                     EMANALIELAGDRELNVLFLCGSAGEIVNLQYEGVEKALEGHDNIKLLDIAFDEGSAA
                     TANQVLTDQAKTHPELNAVICFDSSAATIGTASFIQENKLEEEWITVGIDASADILNY
                     VKAGALDATVNQDFYAMGYNSVKMAYEKIVNGVEPEFINDSGCYLIYPDDVDQYAADN
                     GIDLG"
     gene            1146..2120
                     /locus_tag="ACZPFK_07440"
     CDS             1146..2120
                     /locus_tag="ACZPFK_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406180.1"
                     /translation="MKKKQNSLMDVAMTRPEVGVIAVVVILVAIIGVVNPAFFGGANL
                     MDVLRNTSYAFIIAAPMTLLMMSGGMDLTIGAVTSLGGVACATCLMAGLPGWLSILVA
                     ILFGALVGVLKAVVVVSAGLMPFIATLGLRYVIDGVILVYTRGDPTLIGGASDAFKAL
                     GQGQVLGIYWSFIIAVVVAVLFQIILRNTRYGREVCAVGGNEETARLAGINVKKTRYI
                     INILVSAFAAFVGVIYCARFNSAQPTIGSGTEMTIMASTIIGGASVGGGTGSLFGTFL
                     GTLLLAIIKNGLVLMGVSSYWQNLVFGAILVLSLYVDKVRRSKSGGGL"
     gene            2163..>3408
                     /locus_tag="ACZPFK_07445"
     CDS             2163..>3408
                     /locus_tag="ACZPFK_07445"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1406181.1"
                     /translation="MSELLLEMKDVTKRFPGVTALKDVNIGLQRGEILGICGENGAGK
                     STLMKVLSGSYVAGNYEGEILLDGRPAVFNCVADAQRAGIEMIYQEINVMLDASVAEN
                     IFVGNLPMKHGFVDYKTLYAETQRLLELARMDLSPKMQVRPLNSGQMQMISLLRAYVK
                     KPRILVLDEPTSALTDRETDTLMEILDGLRQNGTSSIYISHKLDEIYRICDRVLVMRD
                     GQTISCRPIAEVQENTLVEEMVGRKVENLYPKVAAEIGEEVLRVEHLTVPHPTIPGKN
                     IINDISFTLNKGEILGIGGLVGAGRSEAVGAVFGQYTHGVSKEVYIHGKKVEIHKPQD
                     AIRAGIGFLTEERKRNGMVWMLSIRENMSLASLPKLSGRIFMNKKKEKEEVSKIFDSL
                     RVKAPSMETRMLALSGGNQQKAI"
BASE COUNT          676 a         1077 c         1083 g          572 t
ORIGIN      
        1 gtgaaaaaca atgaagaaca tgaaaaagct tcttgccgct ctgctcgccg ccgccatgat
       61 ggccgccgcc ctcgcgggct gcggcggttc cgacagctcc agcgctgcgg gcggggcctc
      121 gggcggcgcc gcctccggcg cctcgcagac cgcggccggc gacccgctgg agttctattt
      181 tatcgccctg atgaccggcg gcgccgcgtg gagccgcgcg gaaaaaggct tcaacgacgc
      241 ctgcgccgag ttcggcgtca acggccacta cctggccccc gtcgagcgca acaacgccgt
      301 tgagatggcc gagctgctgg acaaggcgat cgtcgccaag gcggacgcgg tcttcggcgt
      361 cttcctcagc accgatatgt tcggcccggg cctgctgaaa gcgcgggagc agggcgcggt
      421 caccggctcg gtgcagctca ccctgccgga ggagtacatc gacttccaga tcggcaccga
      481 ccagcagcag atcggcgtcg agatggccaa tgccctgatc gagttggcgg gcgacaggga
      541 gctgaacgtg ctgtttctct gcggcagcgc cggagagatc gtcaacctgc agtacgaggg
      601 cgtggaaaaa gcgctggagg ggcacgacaa catcaaactt cttgatatcg ctttcgacga
      661 gggcagcgcc gccaccgcca accaggtgct caccgaccag gcgaaaaccc atcccgagct
      721 gaacgccgtc atctgcttcg acagctccgc cgccactatc ggcaccgcca gctttatcca
      781 ggagaacaag ctggaggagg agtggatcac cgtgggcatc gacgcctcgg ccgacatcct
      841 caactatgtc aaagcgggcg cgctggacgc caccgtcaac caggacttct acgccatggg
      901 ctacaacagc gtcaagatgg cctatgaaaa gatcgtcaac ggcgtcgagc ccgagttcat
      961 caacgactcc ggctgctacc tgatctatcc cgacgacgtg gaccagtacg ccgcggacaa
     1021 cggcatcgac ctcggctgag cctccctccc aaaactgaac ggctgagtat ccccgcgggg
     1081 gccggtggaa agagcgttcc gctccgaccc gccggccccc gcgcaagata aaaggagaga
     1141 aaaccatgaa gaaaaagcaa aacagcctga tggatgtcgc catgacgcgg ccggaggtcg
     1201 gcgtgatcgc ggtggtggtc atcctggtgg cgatcatcgg cgtggtcaac cctgctttct
     1261 ttggcggcgc aaacctgatg gacgtgctgc gcaacaccag ctatgccttt atcatcgccg
     1321 cccccatgac cctgctcatg atgtccggcg gcatggacct gaccatcggc gcggtcacca
     1381 gccttggcgg cgtggcctgc gccacctgcc tgatggccgg gctgcccggc tggctgtcca
     1441 tcttggtggc gatcctcttc ggggcgctgg tgggcgtgct caaggcggtg gtggtcgttt
     1501 cggccggcct gatgccgttt atcgcgaccc tgggcctgcg gtatgtcatc gacggcgtga
     1561 tcctggtcta cacccgtggc gaccccaccc tgatcggcgg cgccagcgac gcgttcaagg
     1621 cgctgggcca ggggcaggtg ctgggcatct actggtcttt catcatcgcg gtggtggtgg
     1681 cggtgctctt tcaaatcatc ctgcgcaaca cccgttacgg ccgcgaggtc tgcgcggtgg
     1741 gcggcaatga ggagacggcc cgcctggccg gcatcaacgt gaaaaagacc cgctacatca
     1801 tcaacatcct cgtctccgct ttcgcggcct ttgtcggcgt catctactgc gcccgcttca
     1861 acagcgccca gccgaccatc ggcagcggca ccgagatgac tatcatggcc tccaccatca
     1921 tcggcggcgc cagcgtgggc ggcggcaccg gctcgctgtt cggcaccttt ttgggcaccc
     1981 tgctgctggc catcatcaaa aacggcctgg tgctgatggg cgtgtccagc tactggcaga
     2041 acctcgtgtt cggcgcgatc ctggtgctct cgctgtacgt ggacaaggtg cgccggtcca
     2101 agagcggcgg cgggctctga gttgccctcc gcggcacaag cggaagaaag cgaggaaaag
     2161 aaatgagcga gctgcttctt gaaatgaaag acgtcaccaa gcggtttccc ggcgtgaccg
     2221 cgctcaagga tgtgaacatc ggcctgcaac ggggcgagat actgggcatc tgcggcgaga
     2281 acggcgcggg caaatccacc ctgatgaaag tgctctccgg cagttatgtg gcgggcaact
     2341 atgaggggga gatcctgctg gacggcaggc ccgccgtctt caactgtgtg gcggacgccc
     2401 agcgcgccgg tatcgagatg atctatcagg agatcaacgt gatgctggac gcctccgtgg
     2461 cggaaaatat cttcgtcggc aacctgccca tgaagcatgg cttcgtggac tacaagaccc
     2521 tctatgcgga gacgcagcgg ctgctggagc tggccaggat ggacctgtcc cccaagatgc
     2581 aggtgcggcc gctgaacagc ggccagatgc agatgatctc gctgctgcgc gcctatgtca
     2641 aaaagccgcg catcctcgtg ctcgacgagc ccaccagcgc gctgaccgac agggagaccg
     2701 ataccctgat ggagattctg gacgggctgc gccaaaacgg cacctccagc atctatatct
     2761 cccacaagct ggacgagatc taccgcatct gcgaccgggt gctggtcatg cgggacggcc
     2821 agaccatctc ctgccgcccc atcgcggagg tgcaggagaa caccctcgtg gaggagatgg
     2881 tgggccgcaa ggtcgaaaac ctctacccca aggtggcggc ggagatcggt gaggaggtgc
     2941 tgcgggtcga gcatctgacg gtgccgcacc ccaccatccc gggcaaaaac atcatcaacg
     3001 acatctcttt cactctgaac aagggcgaaa tcctgggcat cggcggcctg gtgggcgccg
     3061 ggcgcagcga ggccgtcggg gccgtcttcg gccagtacac ccacggggtc agcaaggagg
     3121 tctatatcca cggcaaaaag gtggagatac acaagccgca ggacgccatc cgcgcgggca
     3181 tcggcttttt gaccgaggag cgcaagcgca acggcatggt ctggatgctc agcatccgcg
     3241 agaatatgtc gctggccagc ctgcccaagc tgtcgggcag aattttcatg aacaagaaaa
     3301 aggaaaaaga ggaggtcagc aagatcttcg actccctgcg ggtcaaggcg ccctcgatgg
     3361 agacccggat gctggccctc tccggcggca accagcaaaa ggccatcc
//
LOCUS       DBMDYA010000269         3407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6309_length_3407_cov_1.557471, whole genome shotgun sequence.
ACCESSION   DBMDYA010000269 DBMDYA010000000
VERSION     DBMDYA010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_3407_cov_1.557471"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(238..561)
                     /locus_tag="ACZPFK_07450"
     CDS             complement(238..561)
                     /locus_tag="ACZPFK_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406182.1"
                     /translation="MNFSSAASQLSLGGQSGFEATVLRKAQGEKGRQSTEIAGLFERC
                     EVLKPSLQSQWSLATPKAEAVSGRKELPRTRHKNSRRLFLGHPASPLAGAIEISCFLL
                     QSNKK"
     gene            complement(1223..2062)
                     /locus_tag="ACZPFK_07455"
     CDS             complement(1223..2062)
                     /locus_tag="ACZPFK_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR1406183.1"
                     /translation="MRKTNYHTHTARCFHASGSDEAYVESALKNGYQVLGFSDHSPWK
                     YDSDYVATMRMTPAELPGYLDSIRSLREKYAGRIEILAGLECEYYPRYQDWLRRMVSE
                     NGLDYVIFGNHFYRSDEDGPYFGRATKDRAMLRRYVDSSVEGMESGLYAYFAHPDLFM
                     RCYPGFDADARAASRELSRAAKRLGVPLEYNLMGALYNDNYGVDGYPHEGFWRIAAAE
                     GCTAVIGVDAHAPEQLETDRYRDQGLRLLRGLGMEILDEIPLRTDLRKTEERGPQRDA
                     RRR"
     gene            2248..>3407
                     /locus_tag="ACZPFK_07460"
     CDS             2248..>3407
                     /locus_tag="ACZPFK_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406184.1"
                     /translation="MQMRKNEPFFTHDRSFFSVFLPLTLTVALQNLITHGVNLADNIM
                     LGGYSEAALSGASLANQIQFLLLMLTGGIAEGVVVIGAQYWGRRDTASIKRILRLGLW
                     LSFGVGLIFFLAVSLAPRACLSLFTSDAAVLAEGIRYVRIVCFTYLLFSVTNLLVGAL
                     KCVETVRIGLALSVSTLCINVCLNYCLIFGRFGLPRLGSAGAAVATLVSRAVELIILL
                     VYLLHRDQKLRFRLRELLTPDFSFLPDLTRAGGPIFISNGIWGLAMAVQTAILGHLGA
                     SAIAANSIATTVFQIVSVISYGAAAAAGVTVGKAIGAGRQGELRQYCRTLQVLFLAVG
                     VLEGLVLFFLRDPVLRLYAISGPSLELARQFMLVLCVTVVGTSYQMACLTGIV"
BASE COUNT          619 a         1097 c          973 g          718 t
ORIGIN      
        1 ttttcgctcc ctcgcacccg ataccgccaa aacaagctgc agcgaggcca aaaataaaac
       61 agcggggaca atttttatag tttttcgatg caaaactata tgtccgggat cggtctcggc
      121 ccactgcctt gaactcttaa gcgtccgtcc atcgggtgga cggatgcgtg taaaaaacag
      181 ttgatcttga tggccccggc cagaggaccg gccggatgac ctaaaaatag cctgcggcta
      241 ttttttattg ctctgcaata aaaaacaact tatttctatg gccccggcca gaggactggc
      301 cggatggcct aaaaatagcc tgcggctatt tttatggcgt gtccttggaa gctctttacg
      361 gcctgaaaca gcctccgctt tgggcgtcgc aagactccat tggctttgga gcgacggttt
      421 taacacttcg catctttcaa aaaggccggc aatctctgta gattgccggc ctttttcacc
      481 ctgtgctttc ctcaaaaccg tcgcttcaaa tccgctttgc cccccgaggg agagctgcga
      541 tgcagcactg gaaaaattca cgccgctcgc ctgcttgcgc aggcagctct cagggcgaag
      601 ccgcaagccc ctcagggact ggggggtgaa ttggacggcg ctctgcacgc cgatccttct
      661 ttcgcgggca atcctcttac aggaacgcgc ccttgccccc gtcatccctt gaactcgcgt
      721 agggggcgcg cccggcggcg gagaaacacc cgcttttttc agcacatttt caatggaaaa
      781 aacagtttcc ccgaaaacag gccgttcttc ccgcctcgct ttcaccgcgg cctttcagtg
      841 cctcttgcac cggcaaacct atttctctcg cccgccctaa accgctgggc ccacagcttc
      901 cggcacacta ttttttgcgc tttccaaaat ttcataaaaa atgaatattc aaaaatccaa
      961 aaaagcaaaa tttcgcggcc acacctcccc tgtccacacc aaaagcaggg gggcaaaaaa
     1021 ggggcttgcg gcgctggaac ccagtgacga caaggaacgt ttggtcctgc ccggggaggg
     1081 aatctgcgcg gcctccgctg cgcgcggagc gcggaaaagg ttcggccgca cgaaagcgga
     1141 gcgccgcaag ccggatcggt tcccctacga cgccgacgcg gcgggcgggc cgttgccgtc
     1201 aaaagcaggc ggcacgcgcc ctttatcggc gccgcgcgtc gcgctgcggg cccctttctt
     1261 ccgtttttct caggtcggtg cgcaggggga tctcgtcgag gatctccatg cccaggcccc
     1321 gcagcaggcg caggccctgg tcgcggtagc ggtcggtctc cagctgctcg ggcgcatggg
     1381 cgtccacccc gatgacggcc gtacagccct ccgcagccgc gatgcgccag aagccctcgt
     1441 ggggatagcc gtccacgccg tagttgtcgt tgtagagcgc gcccatcaga ttgtactcca
     1501 gcggcacgcc caaccgcttt gccgcccggc tgagctcacg gctggcggcc cgggcgtccg
     1561 cgtcgaagcc cgggtagcag cgcataaaca ggtcggggtg cgcaaaatag gcgtagaggc
     1621 cgctctccat cccctccacc gagctgtcga cgtaacgccg cagcatggcc ctgtccttcg
     1681 tggcgcgccc aaagtaaggg ccgtcctcgt ccgaccggta aaagtggttg ccgaaaatca
     1741 cataatccag cccgttttcg ctcaccatcc gccgcagcca atcctgatag cgtgggtaat
     1801 actcgcattc caacccggcc aggatctcga tacggccggc atatttttcg cgcagcgagc
     1861 ggatactgtc cagatagccc ggcagctccg cgggggtcat gcgcatggtg gcgacatagt
     1921 ctgaatcata tttccaaggg ctgtggtcag aaaatcccag cacctgatag ccgtttttca
     1981 gcgcgctctc cacataggcc tcgtcgctgc ccgaggcgtg gaagcagcgg gccgtatggg
     2041 tgtgataatt ggtctttctc ataggggctc ctttttaaaa cttctaaaaa acacactgcc
     2101 gcggctcgtc cgggcgcggc cgccgtcttt tattatagcc agggcgaaaa aaaagcaaag
     2161 ggctgtacaa ccgcctcttt ttgcggtata gtcaaaaaaa tgccccgtcc gacggggcgg
     2221 caatttcaac aggctggagg cgcccccatg cagatgcgga aaaacgagcc ctttttcacc
     2281 catgacagat ctttcttttc cgtttttctc cccctcaccc tgaccgtcgc cctgcagaac
     2341 ctgatcaccc acggcgtcaa cctggccgac aacatcatgc tgggcggcta cagcgaagcc
     2401 gcgctctcag gcgcctccct ggccaaccag atccaatttc tgctgctcat gctcaccggc
     2461 ggcattgcag agggggtggt ggtcatcggc gcccagtact ggggacgccg ggacaccgcc
     2521 agcatcaagc gcatcctgcg gctgggactg tggctcagct tcggggtggg gcttattttc
     2581 tttctcgcgg tctcgctggc cccgcgcgcc tgtctctcgc ttttcacctc ggacgccgcg
     2641 gtgttggcgg aggggatacg ctatgtgcgc atcgtgtgtt tcacctatct gctgttcagc
     2701 gtgaccaatc tgttggtggg tgctctgaag tgcgtggaaa cggtgcgcat cggcctggcg
     2761 ctctcggtct cgactctctg catcaacgtc tgcctcaact actgcctcat cttcgggcgg
     2821 ttcgggctgc cgcgtttggg cagcgccggc gcggccgtgg ccacgctggt ctcccgcgca
     2881 gtggaactga tcatcctttt ggtttacctg ctgcacaggg accaaaaact gcgcttccgc
     2941 ctgcgggagc tgttgacgcc ggacttttcc tttttgcccg atctcacccg cgccggcggc
     3001 cccatcttta tctccaacgg catctggggg ctggccatgg cggtgcagac ggcgatcctg
     3061 ggccacctcg gcgccagcgc catcgccgcc aacagcatcg ccaccactgt ctttcagatc
     3121 gtcagcgtca tcagctacgg cgcggcggcg gccgcgggcg tgacggtggg caaggccatc
     3181 ggcgcggggc ggcagggcga gctgcgccaa tactgccgca ccctgcaggt gctttttctg
     3241 gcggtggggg tccttgaggg gctggtcctg tttttcctgc gcgaccccgt gctgcgcctg
     3301 tacgctatct ccggcccctc gctggagctg gcgcgccagt tcatgctggt gctctgcgtc
     3361 accgtggtcg gcacctccta ccagatggcc tgcctcaccg gcatcgt
//
LOCUS       DBMDYA010000270         3377 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6372_length_3377_cov_1.548230, whole genome shotgun sequence.
ACCESSION   DBMDYA010000270 DBMDYA010000000
VERSION     DBMDYA010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6372_length_3377_cov_1.548230"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /gene="pknB"
                     /locus_tag="ACZPFK_07465"
     CDS             complement(<1..2090)
                     /gene="pknB"
                     /locus_tag="ACZPFK_07465"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1406185.1"
                     /translation="MDMYIGKRLDGRYEIETLIGVGGMANVYRAKDLLEDRAVAVKIL
                     RDEFLSNEDLVRRFKNESKAISLLSHPNIVRVFDVSVSDKVQYIAMEYIDGVTLKDYI
                     EQRKVLTWKETLHFITQTLEALQHAHNKGIVHRDIKPQNIMVLADGSIKVMDFGIARF
                     SRGESHTTTDKAIGSVHYISPEQAKGEAIDLRADLYSTGVMMYEMLTGKLPFESDSAV
                     SVAIKQISDEAVPPRKLNPEIPEALEAITMKAMAKDVRHRYQTAGEMLGAIEEFKKNP
                     SVRFEYEYLKEDSPTRYIDKVVNKSKRSQPAPRGKKPAQAQGPKKSKLRFTVPILAGM
                     AVAFAVGAGILCFLIFKTSGLFSTKPDVEVSTFTGMTVNEAKQNGNFKLVTVEVYNAN
                     VEAGVIFDQSPKPPKIVKQGSKITVKVSLGKQEVKVPELSGLTHGEAEKALTELGLMV
                     SIVPEQNDNVQEGKVIRTSPEKGTKLESGQTVVLYVSRAARSEKVKVPDLTGLTSEAD
                     ASKVLEPLRLRLGTRTTVENTAPAGTIIGQDPMGGTEVAVGSKVNIQVSSGVVEPTER
                     HIAVTITFDQYVPDGHWSADFPGGHSEFDVTKDNRVWTFTAVGTRGPSTITLSNGMQI
                     GIDFDTETAKNIEVNGTPPTPTPPPTPTPTPTATPTPKPTATPTPKPTATPTPKPTAT
                     PTPKPTATPTPKPTA"
     gene            complement(2093..2836)
                     /locus_tag="ACZPFK_07470"
     CDS             complement(2093..2836)
                     /locus_tag="ACZPFK_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1406186.1"
                     /translation="MKIVGKTDIGKRRTENQDNYRAGRQSDDTIWAVVCDGMGGARGG
                     AVASSIAVSVLEEEFVRGLLPGLHSAAIRELMERAAQHANREIYDLACADPSKRGMGT
                     TMVAMVLKNGLAHIVHAGDSRAYLYRHGRLERLTRDHSMVQEMVENGSLTPEEAEVHP
                     NKNLITRAVGVNPDLAVEYGERQALPGDILLLCSDGLTNFTSEEELCGILESSEFFGA
                     ADAMIDAALAAGGQDNITAVLIKVEATED"
     gene            complement(2908..>3377)
                     /locus_tag="ACZPFK_07475"
     CDS             complement(2908..>3377)
                     /locus_tag="ACZPFK_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046812.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1406187.1"
                     /translation="STCGLVPQIGRLAEEKLGLTLSISLHAPTDALRSSMMPVNDAYP
                     LAQLIPACRAYQKITGRRISFEYSMVKGVNDSPECARQLAQLLKGMGAHVNLIPINPV
                     DGSPYTASDAANVERFKDLLGRLGLNATVRRRLGTDISAACGQLRQEEARRPG"
BASE COUNT          501 a         1090 c         1077 g          709 t
ORIGIN      
        1 gccgtcggct tcggggtcgg ggtcgccgtc ggcttcggcg tcggggtcgc cgtcggcttc
       61 ggcgtcgggg ttgccgttgg cttcggcgtt ggcgttgctg tcggcttcgg cgtcggcgtc
      121 gccgtcggcg tcggggtcgg ggtcggcggc ggggtgggcg tgggaggcgt gccgttgacc
      181 tcgatgttct ttgcggtctc ggtgtcaaaa tcgatgccga tctgcatgcc gttggacaag
      241 gtgatcgtgg acggacccct ggtgcccact gcggtgaaag tccacacgcg gttgtccttg
      301 gtcacgtcga actcgctgtg gccgccgggg aaatccgctg accagtgccc gtccggcaca
      361 tactggtcaa aggtgatggt gaccgcgata tggcgctcgg tgggctccac cacgcccgag
      421 ctcacctgga tattgacctt gctgcccacc gcgacctcgg tgccgcccat cgggtcctgg
      481 ccgatgatgg tacccgctgg ggccgtgttc tcgacggtgg tgcgggtgcc caaccgcagg
      541 cgcagcggct ccaagacctt ggaggcgtcc gcctcgctgg tcaggccggt cagatcgggc
      601 accttgacct tttcactgcg cgccgcacgg ctgacataca gcaccacggt ctggccgctc
      661 tcgagcttgg tccccttttc cgggctggtg cggatgacct tgccctcctg cacattgtcg
      721 ttctgctcgg gcacgatgct gaccatcagc cccagctccg tcagcgcctt ttccgcctcg
      781 ccgtgggtca ggccggagag ctccggcacc ttgacctcct gcttgcccag gctcaccttg
      841 acggtgatct tggagccctg cttgacgatc ttcggcggct tggggctctg gtcaaaaatg
      901 acccccgcct ccacgttggc gttgtagacc tccacggtca caagtttaaa attgccgttc
      961 tgcttggcct cgttcaccgt catgccggtg aacgtgctca cctccacgtc cggctttgtg
     1021 ctgaacaggc cgctggtctt gaagatcaaa aagcagagga tgcctgcccc caccgcaaat
     1081 gccaccgcca tgcccgccag aatcggcacg gtgaagcgga gcttggattt ttttgggccc
     1141 tgcgcctggg cgggcttttt gccgcggggg gcgggctgcg atcttttcga cttgttaacc
     1201 actttatcaa tatacctcgt cggagaatcc tctttcagat attcgtactc aaaccggacg
     1261 ctgggattct ttttgaactc ctcgatggcg cccagcatct cgcccgccgt ctgatagcgg
     1321 tgccgcacgt ccttggccat ggctttcatg gtgatggcct ccagcgcctc cggaatctcg
     1381 gggttgagct tgcggggcgg cacggcctca tcggagatct gcttgatggc gaccgagacg
     1441 gcgctgtcgc tctcgaacgg cagcttgccg gtgagcatct catacatcat cacgccggtg
     1501 gagtagagat cggcccgcag gtcgatggct tcgccctttg cctgctcagg gctgatgtaa
     1561 tgcaccgagc cgatcgcctt gtcggtggtg gtgtgggact ccccgcggga aaagcgcgcg
     1621 atgccgaaat ccatgacctt gatcgacccg tccgccagca ccatgatgtt ctggggcttg
     1681 atgtcccggt gcacgatgcc cttgttgtgg gcgtgctgca gggcctccag cgtctgggtg
     1741 atgaagtgga gggtctcttt ccaggtcagc accttgcgct gctcgatata atctttcagg
     1801 gtgacgccgt cgatatactc catggcgatg tactgcacct tgtcggacac gctgacgtcg
     1861 aacacccgca caatattggg gtgcgagagc agcgagatgg ccttggactc gttcttgaac
     1921 cggcgcacca aatcctcgtt ggagaggaac tcgtcccgca ggatcttgac cgccacggcg
     1981 cggtcctcca gcaggtcttt cgccctgtag acgttggcca tgccgccgac gccgatcagc
     2041 gtctcgatct catagcggcc gtccagccgc ttgccgatat acatatccat gctcaatcct
     2101 ccgtcgcctc gaccttgatc agcaccgcgg tgatgttgtc ctgcccgccg gcggcaagcg
     2161 ccgcgtcgat catcgcatcc gccgcgccga agaattcact gctttccagg atgccgcaaa
     2221 gctcttcttc actggtgaaa ttggtcagcc cgtcgctgca cagcagcagg atgtctcccg
     2281 gcagcgcctg gcgctcgccg tactccaccg caaggtccgg gttgaccccc accgcacggg
     2341 tgatcaggtt cttattgggg tgtacctccg cttcctccgg ggtcagcgag ccgttctcca
     2401 ccatctcctg caccatcgag tggtcgcggg tgagccgctc taggcggccg tgccggtaga
     2461 gataggcgcg ggaatcgccc gcgtggacga tgtgcgccag cccgtttttg agcaccatgg
     2521 ccaccatggt ggtccccatc cctcgcttgg agggatcggc acaggccagg tcgtagatct
     2581 cgcggttggc gtgctgcgcc gcccgctcca tcagctcgcg gatggccgcc gagtgcagcc
     2641 ccggcagcaa gccgcggacg aactcttcct ccagcacgct cacggcgatg ctgctggcca
     2701 ccgccccgcc gcgcgcgccg cccatgccgt cgcagacaac ggcccagatg gtatcgtcgc
     2761 tctggcggcc ggcgcggtaa ttgtcctggt tttcggtgcg gcgcttgccg atatcggtct
     2821 tgcccacgat cttcatggtg gtcactccct ccttagggct gttctcccgc ctcgctgcgg
     2881 gaacaggggt ctgatattga aaaggattca gccgggccgg cgggcctcct cctgccgcag
     2941 ctggccgcag gccgcgctga tatcggtgcc caggcggcgg cggacggtgg cgttgaggcc
     3001 gagacggccc agcagatcct tgaaccgctc cacattggcg gcgtcgctgg ccgtataggg
     3061 cgacccgtcc accgggttga tggggatcag gttgacatgg gcccccatgc ctttcagcag
     3121 ctgcgccagc tgccgcgcgc actcggggct gtcgttgacc cctttgacca tcgaatactc
     3181 aaagctgatg cgccggccgg tgatcttttg gtaagcgcgg caggcgggga tcagctgcgc
     3241 cagggggtag gcgtcgttca ccggcatcat ggaagagcgc agggcgtcgg tgggcgcgtg
     3301 cagcgagatg gaaagggtca ggcccagctt ttcctccgca aggcggccaa tctgcggcac
     3361 cagaccacag gtggaca
//
LOCUS       DBMDYA010000271         3376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6374_length_3376_cov_1.497099, whole genome shotgun sequence.
ACCESSION   DBMDYA010000271 DBMDYA010000000
VERSION     DBMDYA010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_3376_cov_1.497099"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="ACZPFK_07480"
     CDS             complement(<1..1270)
                     /locus_tag="ACZPFK_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR1406188.1"
                     /translation="MFIADLHIHSHYSRATSRDCVPEQLDLWARRKGIHLVGTGDFTH
                     AAWREELAQRLEPSGEGFYRLRPEHRLRDRAAAAAPDPRFVVTGEISSIYKKNGRVRK
                     VHNVILLPSLEAAAALSARLEGLGCNLHSDGRPILGLDSRDLLELTLDACPEAVFIPA
                     HIWTPHFSLFGAFSGFDTIEECFEDLTPHIHALETGLSSDPPMNWRLSALDRYFLVSN
                     SDAHSPAKLGREANLFDAELSWGGLSGALSGGGRGPRSTIEFFPEEGKYHFDGHRNCG
                     LRLTPAEAEAAGGVCPVCGRKLTTGVLHRVEQLADREEDFRRPDAAPFERIVPLPEII
                     GASVGRSAAGAAVERAYEAMLAELGPEFSILCELPLEDIRRVAGPCVAEGVRRVRAGE
                     LHWEPGFDGEYGRVEILSRDEIGAFSGQLSF"
     gene            1523..1867
                     /locus_tag="ACZPFK_07485"
     CDS             1523..1867
                     /locus_tag="ACZPFK_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406189.1"
                     /translation="MTEKEDAKLRGMMLWMAECPDRYSAICGEDYEPDAEKFLQLLEE
                     LEERGYYELMLIWTMKNNINNMVDRALSRMIADEIARAWQQEDAARLSSRFKSLLREE
                     IRRAGEKENERG"
     gene            complement(2022..>3376)
                     /locus_tag="ACZPFK_07490"
     CDS             complement(2022..>3376)
                     /locus_tag="ACZPFK_07490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1406190.1"
                     /translation="APAGGAACTRKKGGPTMNKNPPRRARALTVGLVALLLAALAAAL
                     VLWRQWAAAGEGSVPAVSVTLDGEELLQHGGSWETPLLGGLLTRRPTLSYPALGKVLA
                     LDSDQPLLAAAPADAALSRVRVSRGGDALFDGSPEAFAAFRFPGDGDYFVEVWGSLDR
                     GGDGRGDFYFDASVALLLPVPEPEFSLSSADAQQGDLLTVTARHIPEGVTPSGESGLG
                     YVRFVPAGEPGVYTALVPVGYAREPGAYTIEVTAGQASASLPVTVAESDFEVQQMTIS
                     EEIADETVNSQAANWEFHVTVVPLYDTANDERYWSVPFLQPVEGRISTPYGVKRYVNG
                     GATPERHGGVDIAAALGTPVQCPADGLVEYAGFLQLSGNTIVIEHGGGLKSYFYHMDS
                     LDVATGDRVEKGRLLGAVGTTGYSTGPHLHYEVKIGRESVSPWPLFDGSSAIFAADAG
                     "
BASE COUNT          603 a         1091 c         1177 g          505 t
ORIGIN      
        1 cgaacgagag ctggcctgaa aaggcgccga tctcgtctcg gctgaggatc tccacccggc
       61 cgtattcgcc gtcaaagccg ggctcccagt gcagctcgcc ggcccgcacc cggcgcacgc
      121 cctcggcgac gcaggggccg gccacccggc ggatgtcctc cagcggcagc tcgcacagga
      181 tggagaactc cggccccagc tcggccagca tggcctcgta ggcccgctcc acggcggccc
      241 cggcggccga gcggcccacc gaggccccga tgatctcggg cagcggcacg atgcgctcaa
      301 agggggcggc gtccgggcgg cggaaatcct cctcccggtc ggccaactgc tccacccggt
      361 gcagcacgcc ggtggtgagc ttgcggccgc agaccgggca gaccccgccc gccgcctcgg
      421 cctcggccgg ggtcagccgc aggccgcagt tgcggtgccc gtcaaagtgg tatttgccct
      481 cctcggggaa gaactcgatg gtggagcggg ggccccggcc cccgcctgag agcgcgcccg
      541 agaggccgcc ccaggacagc tcggcgtcga agaggtttgc ctcccggccc agcttggccg
      601 gggagtgggc gtccgagttg gagaccaaaa agtatcggtc cagcgccgag agccgccagt
      661 tcatgggcgg gtcggaggag agccccgtct ccagcgcgtg gatgtggggc gtcaggtcct
      721 caaagcactc ctcgatggtg tcgaagcccg aaaaggcgcc gaagagcgaa aagtggggcg
      781 tccagatgtg ggccgggatg aacaccgcct cggggcaggc gtccagcgtc agctccagca
      841 ggtcgcggct gtccaggccc agaatgggcc gcccgtccga gtgcaggttg caccccaggc
      901 cctccagccg ggccgagagg gcggccgcgg cctcgaggga gggcagcagg atgacgttgt
      961 gcaccttgcg cacccggccg ttctttttgt agatggagct gatctcgccg gtgaccacaa
     1021 agcgcggatc cggcgcggcg gccgcggcgc ggtcccgcag gcggtgctcg ggccgcaggc
     1081 ggtaaaagcc ctcgcccgac ggctccagcc gctgggccag ctcctcccgc caggcggcgt
     1141 gggtgaagtc gccggtgccc accaggtgga tgcccttgcg gcgggcccag aggtccagct
     1201 gctcgggcac gcagtcgcgg ctggtggcgc gggagtaatg ggagtggatg tgcaggtcgg
     1261 cgatgaacat aggggcacct cgcttgcggg ccgcggcggg cgcgccgcgt gccggaatct
     1321 gtcgatttgt ccctatcata tcgtttttga cggccccgcg caagcgggga aaccaggcaa
     1381 aagggagacg ccctgcggcg tagagagatt cagaaaattt taaaatggat actgcccggt
     1441 atccctttgc ccgctttttc agtgctattc tgggaattgg gcggccggac cgccgagaat
     1501 aggctgaaag gagagcggga ggatgacgga gaaagaggac gcaaagctga gagggatgat
     1561 gctgtggatg gcggaatgcc ccgaccgata cagtgcgatt tgcggcgagg actatgagcc
     1621 ggatgcggaa aaatttttgc agttgctgga agagctcgaa gagcggggct attatgagct
     1681 gatgctgatc tggacgatga aaaataacat caacaacatg gtagaccggg cgctgtcccg
     1741 aatgattgcg gacgaaatag cgcgggcttg gcagcaagag gacgcggccc ggcttagcag
     1801 tcggttcaaa tcgcttttgc gggaggaaat acggcgggca ggtgaaaaag aaaatgaaag
     1861 gggctgagaa aggaacggtc aatttctgcc tttgttctca gcatgcaaca tgaaacggtt
     1921 ttaccttttt gcaaaatgaa aatgggcggc aagaatttct gccgcccatt tttatcagta
     1981 aaaaaagatc gaaaaatcag aaaaatggat ggcgcccgcg ctcagcctgc gtccgccgcg
     2041 aagatggccg agctgccgtc gaaaaggggc cagggggaga cgctctcgcg cccgatcttc
     2101 acctcgtagt gcagatgcgg gccggtggaa tagccggtgg tgcccaccgc gcccagcagc
     2161 cgcccttttt ccacccggtc gccggtggcc acgtccaggc tgtccatgtg gtaaaaatag
     2221 cttttgaggc cgccgccgtg ctcgatgacg atggtgttgc ccgacagctg caaaaagccg
     2281 gcgtattcca ccaagccgtc ggccgggcac tgcaccggcg tgcccagggc cgccgcgatg
     2341 tccacgccgc cgtggcgctc cggcgtggcg ccgccgttca cgtaccgctt caccccgtag
     2401 ggggtggaga tgcgcccctc taccggttgt agaaatggga cgctccaata ccgttcgtcg
     2461 ttggcggtgt cgtagagcgg caccaccgtg acgtgaaatt cccagttggc ggcctgcgag
     2521 ttcaccgtct cgtcggcgat ctcctcgctg atggtcatct gctgcacctc aaagtcgctc
     2581 tccgccaccg tcaccggcag cgaggcggag gcctgcccgg ccgtgacctc tatagtgtag
     2641 gcgcccggct cgcgcgcata gcccaccggc accagcgccg tgtaaacgcc cggctcgccg
     2701 gcgggcacaa agcgcacgta tccgagaccg ctctcaccgc tgggcgtcac cccctcgggg
     2761 atgtggcggg ccgtcacggt gagcaggtcg ccctgctgcg cgtcggcgga ggagagcgaa
     2821 aactccggct cgggcacggg cagcagcagg gccaccgagg cgtcgaagta aaagtcgccg
     2881 cgcccgtccc cgccccggtc caggctgccc cagacctcga caaagtagtc cccgtcaccg
     2941 ggaaagcgga acgccgcgaa cgcctcgggg ctgccgtcga aaagggcgtc gccgccccgc
     3001 gacacccgca cccggctgag cgccgcgtcc gcgggcgcgg cggccaaaag aggctggtcg
     3061 ctgtccagcg ccagcacctt gccgagggcg gggtaggaga gagtcggccg ccgggtcagc
     3121 agaccgccca gcagcggcgt ctcccagctg ccgccgtgct gcagcagctc ctcgccgtcc
     3181 agcgtcaccg aaacggcggg caccgagccc tcgccggcgg ccgcccactg gcgccagagc
     3241 accagggcgg cggccagcgc ggccagcagc agcgccacca gcccgacggt gagcgcacgg
     3301 gcgcggcgcg ggggattttt gttcatggtg ggacctcctt ttttgcgggt gcaggcggcg
     3361 ccccctgccg gggcag
//
LOCUS       DBMDYA010000272         3375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6376_length_3375_cov_1.818265, whole genome shotgun sequence.
ACCESSION   DBMDYA010000272 DBMDYA010000000
VERSION     DBMDYA010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_3375_cov_1.818265"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="ACZPFK_07495"
     CDS             complement(<1..358)
                     /locus_tag="ACZPFK_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406191.1"
                     /translation="MAIGQRDDGIIIGVSLDTARVKKATEQMNRDFASFGSSSAKAIG
                     QVDKSLDEARKNAESWKIEPTTDGIQAAAKELDHLNAVIVNQQAELANCEREHERVAE
                     RYGETSNQALKLEKRML"
     gene            complement(336..896)
                     /locus_tag="ACZPFK_07500"
     CDS             complement(336..896)
                     /locus_tag="ACZPFK_07500"
                     /inference="COORDINATES: protein motif:HMM:NF018553.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HIR1406192.1"
                     /translation="MLDLPREILGQAVETDWMWWMRRVGYVLDSSLLWAEKADILLLN
                     VYRFIPDDPKQAIRRLQGIFDFYLCGDDGEPDEEPPREKLLDWQKDGARIWGDFRVYA
                     GIDLNTARMHWWEFMAIFKSLPPDSQIKYAIALRALDLSEIEDTKQREHYARAKRAVA
                     LEPMDYEREYDAAMERRTGLGNRTEG"
     gene            complement(781..1335)
                     /locus_tag="ACZPFK_07505"
     CDS             complement(781..1335)
                     /locus_tag="ACZPFK_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406193.1"
                     /translation="MGFEFSPKHMIEICGKQYPCDIGDCRMLEGVARDFPRILRAAQE
                     LLQVDAKLKTAVKEKQPTAELADEVIRCNGELLQACRDFIEGTLGESEYQEIFADRPA
                     NTEEHIELCAYIYSDIMGGRQEIVNEYLDPAVREEGQTTNGAGSDAGSPPGDIGAGGG
                     DGLDVVDAPRGIRARLLIALGGKS"
     gene            complement(1350..1856)
                     /locus_tag="ACZPFK_07510"
     CDS             complement(1350..1856)
                     /locus_tag="ACZPFK_07510"
                     /inference="COORDINATES: protein motif:HMM:NF047353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098026 - virus tail, tube [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR1406194.1"
                     /translation="MPGTYVKPKAYNRRWWIDISANDTPQWAELAIGITSRGNSISES
                     KDDYYDMAGRGVAESEISGITVQRTFSGFRVMGDEAQDNIIERLYDLDHRAVKFLECY
                     DNPAAAGALKGRQGMATLSITDDGSGDANKRENIAFALNIEGTPEKGTIVIAADGVPT
                     FTADAAGQ"
     gene            complement(1861..2274)
                     /locus_tag="ACZPFK_07515"
     CDS             complement(1861..2274)
                     /locus_tag="ACZPFK_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406195.1"
                     /translation="MSILSDLRKLFEGCPLLAELTQRVDALGTRVEEYGIYPAGSNVV
                     SEDMAGAKTWQYNFVLQAERMTVDDLTRLENNDFIEQLQDWVEAQNRDGVPLTDEGVD
                     FISISAGNGAIVDWDDREQYGTYKVQCTLIIEKEG"
     gene            complement(2279..2686)
                     /locus_tag="ACZPFK_07520"
     CDS             complement(2279..2686)
                     /locus_tag="ACZPFK_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406196.1"
                     /translation="MSMLQIDIRMPELQTLVQDLGLAPGGPVQQDLVLNVARRITKYV
                     PKRTYSSVEHAIAQGAEPRNGRIVIRGPHIRYLYFGKVMAGRKPKKVTDKDLVYTKTY
                     NRLAGPRWMERLLAAEYDQIIADMENFIRAVGR"
     gene            complement(2683..2994)
                     /locus_tag="ACZPFK_07525"
     CDS             complement(2683..2994)
                     /locus_tag="ACZPFK_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406197.1"
                     /translation="MLTPNASCTLYLQTGPGAYTRVYVPVCFWQDREDGASIIIPGEL
                     PEQYKGEKREQDYVVRGKRTGEVTDTQSKRELIADKPLTVEHLARHLYGGLPHCEVTA
                     K"
     gene            complement(2988..>3375)
                     /locus_tag="ACZPFK_07530"
     CDS             complement(2988..>3375)
                     /locus_tag="ACZPFK_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR1406198.1"
                     /translation="RMVADKEFYYATYHGKLPQADVEGCLSRAEYMLHNLTLDRLQDG
                     AWEQDEKLAKCVRMAHCALADAQHAQDTAVLAGGKVTSESVGNWSRSVQQDDAQSGSF
                     ERRCLRIAAQYIPIRSGLLYRGTSRC"
BASE COUNT          583 a         1099 c          873 g          820 t
ORIGIN      
        1 tgagcatccg cttttccagc ttcagcgcct ggttgctggt ctcaccgtac cgttctgcca
       61 cacgctcatg ctcccgctcg cagttggcaa gctccgcctg ctggttcacg atgacagcgt
      121 tcagatggtc cagctcttta gccgcagcct ggatgccgtc cgtggtgggc tcgatcttcc
      181 agctctctgc gttcttacgg gcttcatcca gggatttgtc cacctggcca atggcttttg
      241 ccgacgacga cccaaacgat gcaaagtccc gattcatctg ctccgtcgct tttttgaccc
      301 gggccgtgtc caggctcaca ccaatgatga tgccgtcatc cctctgtccg attgccaaga
      361 ccggtcctcc tctccatagc tgcgtcgtac tctctttcgt aatccatcgg ctccagagct
      421 actgcccgtt tcgcacgggc gtagtgctcc cgctgctttg tatcctcaat ctcggataga
      481 tccaaagcgc gcagcgctat agcatacttg atctggctgt caggcggtaa gctcttgaag
      541 atcgccataa actcccacca gtgcattcgt gcggtattga ggtcaatacc ggcgtacacc
      601 ctgaaatccc cccatattcg tgccccgtcc ttttgccagt ccagcagttt ttcacgaggc
      661 ggctcctcgt ccggctcacc gtcgtcgccg cacaaataaa aatcgaatat tccttgcaga
      721 cgtctaattg cttgctttgg gtcatctggg atgaaccggt acacattgag cagcaagata
      781 tcagcttttt ccgcccaaag caatgaggag tcgagcacgt atcccacgcg gcgcatccac
      841 cacatccagt ccgtctccac cgcctgcccc aatatctccc gggggagatc cagcatcaga
      901 ccctgctcca tttgttgtct gcccctcctc ccggactgcc gggtcgagat actcgttgac
      961 gatctcctgg cggcccccca tgatatccga gtagatatac gcgcacagct caatatgctc
     1021 ctctgtgttg gctggccggt ccgcaaaaat ctcctggtat tccgactcgc ccagcgtgcc
     1081 ctcgataaaa tcccgacaag cctgcagcag ctcgccattg cagcggataa cctcatccgc
     1141 cagctccgct gtgggctgct tttcttttac ggccgtcttt agtttggcgt caacctgcag
     1201 cagttcctgg gcggcgcgca ggatgcgcgg aaaatcccgc gccaccccct ccagcatccg
     1261 gcagtccccg atatcgcagg gatattgctt gccgcagatc tcgatcatgt gctttggaga
     1321 aaattcaaac cccatactgc cgtctccttt tactgcccgg cggcgtctgc cgtaaaggtc
     1381 gggacgccgt cagccgcaat gacaatggta cctttttccg gcgtcccctc gatgttgagg
     1441 gcaaaggcga tgttttctcg cttgttggcg tcgccgctgc cgtcgtccgt aatggagagg
     1501 gtcgccatac cctgacggcc cttgagcgct cctgcggccg cggggttgtc gtaacactcc
     1561 aaaaacttga cggcccgatg gtccaggtca tacaggcgct cgatgatatt gtcctgcgct
     1621 tcgtcgccca tcacccggaa ccccgaaaag gtccgctgta ccgtgatgcc ggagatctcc
     1681 gactccgcca cgccgcggcc tgccatgtca tagtagtcat ctttgctctc gctgatgctg
     1741 ttgccgcggg aagtgatccc gatggcgagc tctgcccatt ggggcgtgtc atttgcgctg
     1801 atgtcgatcc accagcggcg gttatatgct tttggtttta cataagttcc aggcatattc
     1861 ttaaccctct ttctcaatga ttaaggtgca ttgtaccttg taagtaccgt attgctctct
     1921 gtcatcccag tccacgatgg ccccgttgcc ggcggaaatg gatataaaat ccactccctc
     1981 atcagtgaga ggcacgccgt cccggttttg cgcttctacc cagtcctgta actgttcgat
     2041 gaaatcgttg ttctctaggc gcgttaggtc atccactgtc attcgctccg cctgcagcac
     2101 aaagttatac tgccaggtct tcgccccggc catatcctct gagacgacat tactgccggc
     2161 tggatagatg ccgtattctt ccaccctggt tcccaatgcg tccactcgct gcgtcagctc
     2221 tgccaacagg gggcagccct caaacagctt gcgcagatcg cttaaaatcg acatatcctt
     2281 acctccctac ggcgcggata aagttctcca tgtccgcgat gatctggtcg tattcggcag
     2341 ccagaagccg ttccatccag cgcggcccgg cgagtcggtt atacgttttg gtatagacca
     2401 ggtccttgtc agtcaccttt tttggcttgc ggcccgccat gaccttgccg aagtacaggt
     2461 acctgatgtg cggaccgcgg atcacaatgc gcccgttacg gggctctgcg ccctgggcga
     2521 tggcgtgctc cacactgctg taagtgcgtt tgggcacata tttcgtgatg cgccgtgcca
     2581 cgttgagcac aaggtcctgc tgcaccgggc cgccgggcgc aaggcccagg tcctgcacca
     2641 acgtctgcag ctccggcatc cgtatgtcga tctgcagcat gctcattttg ccgtcacctc
     2701 gcaatggggc aggccgccgt acaggtggcg cgccagatgt tctacggtca aaggcttgtc
     2761 tgcgatcagt tcccgcttgc tctgtgtatc cgtgacctca cccgtgcgct tgcctcgcac
     2821 cacgtagtcc tgttcccgtt tttcaccttt gtactgctcc ggcagctcgc cggggatgat
     2881 aatgctcgcg ccgtcctctc tgtcctgcca gaagcagacg gggacataca cccgtgtgta
     2941 tgcccccggc cccgtctgca gatacagcgt gcagcttgcg ttcggcgtca gcatctgctc
     3001 gtcccccggt acagcagccc gctgcggatg gggatatatt gggcggcgat acgcaggcag
     3061 cggcgctcga agctgccgga ctgtgcgtca tcctgctgta cgctgcggct ccagtttccc
     3121 acgctctcgc ttgtcacttt gccgcccgca agcaccgccg tgtcctgggc gtgctgggcg
     3181 tccgccagtg cgcagtgcgc catgcgcacg catttcgcca gcttttcgtc ctgctcccag
     3241 gccccgtcct gcaggcggtc cagtgtcaga ttgtgcagca tgtactcagc gcgggacaga
     3301 cagccttcta catccgcctg tggcagcttg ccgtgatatg tggcataata gaactccttg
     3361 tccgctacca tgcgt
//
LOCUS       DBMDYA010000273         3372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6386_length_3372_cov_1.340569, whole genome shotgun sequence.
ACCESSION   DBMDYA010000273 DBMDYA010000000
VERSION     DBMDYA010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_3372_cov_1.340569"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="ACZPFK_07535"
     CDS             complement(<1..277)
                     /locus_tag="ACZPFK_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1406199.1"
                     /translation="MHCTRKLTDTVLWVGGCDRRLALFENLFPVPQGITYNSYLILDE
                     KAALIDTVDASISRQFLENILYALAGRPLDYLVIDHMEPDHCANIGEL"
     gene            870..>3372
                     /locus_tag="ACZPFK_07540"
     CDS             870..>3372
                     /locus_tag="ACZPFK_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR1406200.1"
                     /translation="MQIKKRSGALEEYNFAKITGAIKKAFLSVGQQVGEAVLADLLAG
                     VESRLPGGEISVEQIQDLVERVMMEQGHYDAAKSYILYRQKRAELRSARQALARQIGV
                     EGLEGCLAEIQTEFDSALYPLTALSTQFAGFAKPGMTQAELLAVLTKAAVELTSPEAP
                     QWELIAARFLNFSFRLQLEEELLRRGIGSFYEKLVYLTREGLYGEYILQNYTKAELEE
                     AAGFIRQKRDGRFTWSGLDLLLKRYVIRTRQHVALETPQEMFLGIALHLAMNEKQGRM
                     AWVRRFYDMLSLMQVTMATPTLSNARKPYHQLSSCFIDTVPDSLEGIYRSIDSFAKVS
                     KFGGGMGLYFGKVRATGSSIRGFEGAAGGVIRWIRLVNDTAVAVDQLGMRQGAVAVYL
                     DAWHRDLPEFLQLRTNNGDDRMKAHDVFPAVCWPDLFWKRASQDLDSPWHLMCPHDIL
                     TVKGYALEDYWGEEWERRYLSCVEDPRIPKRTVTVKEIVRLVLKSAVETGTPFTFNRD
                     AVNRANPNGHKGMIYCSNLCTEIAQNMAPVESVSTEVTTREGDTVVVTTTRPGEFVVC
                     NLASLSLGNLPVEDEAAMGEIVRTAVRALDNVIDLNFYPLPYARLTNQKYRSIGLGVS
                     GYHHMLAKHGVRWESEEHLAFADRVFETINRAAIIASCELAEEKGAYSLFPGSDWATG
                     AYFEKRGYTGPAWRALAERVRRSGMRNAYLLAVAPTSSTSILAGTTAGIDPVMNKFFL
                     EEKKGSMLPRVAPELSQATYWYYKNAHLIDQRWSVRACGVRQRHIDQAQSMNLYITNE
                     YTLRQVLDLYLLAWKQGVKTIYYIRSKSLEVEECES"
BASE COUNT          732 a          953 c         1054 g          633 t
ORIGIN      
        1 gcagttcacc gatattggcg cagtggtcgg gctccatgtg gtcgatcacc agatagtcca
       61 gcggccggcc tgccagggcg tataaaatat tctccagaaa ttgccgggag atggaggcgt
      121 ccaccgtgtc gatgagggct gctttttcat ccagaatcag atacgaattg taggtgatcc
      181 cctgcggaac aggaaaaagg ttctcaaata aagctaaacg gcggtcacag ccgcccaccc
      241 agaggacggt atcggtcagt tttcttgtac agtgcatggc aatgcccctt tcgacagtgt
      301 gaaagacccg cttgagaacc cgcggcaaag ctcccggcag ggtcctcttt tcactcagta
      361 gtatgcccgc ctgagccgtc tcttttcacc gtcagccgaa agagacgccg cgctttggcc
      421 ggggccgcct cagaaaaaga aaactcagca gtcctatttt tacgcatgac ggcttccaac
      481 cggtggggcg ctccggctta ggccccggct ttggccgggc aagagacatt tttgtatctg
      541 aacgtttttc ccggcgccgt ccctttctga acgctgcggc gcgatacctt gcctccaaaa
      601 taaaatgcag gaataaaaag cggcggcggg gatacccgcc gggctgaccg atgccaaaga
      661 caaatttcca ctgcataaaa gggcatcggc gggagaggct ttttaaataa aaaagaaaaa
      721 agcccccgct ttgccgagaa taaaaaatgc cggataattt tatactattt ttgtatattt
      781 taatataaaa tgtgttttgc tcttgcaaag gcacaagata cagtatatta ttgagataat
      841 ttcctgtttg tggcaggagg aggagcaaaa tgcagatcaa aaaaagaagc ggtgcgctgg
      901 aggagtacaa cttcgccaag attacggggg ctataaaaaa ggcttttctc agcgtggggc
      961 aacaggtggg ggaagccgta ttggccgacc tgctcgccgg cgtggaaagc cgtctgcccg
     1021 ggggcgagat ctcggtggaa cagatccagg acctagtcga acgggtgatg atggagcagg
     1081 ggcactacga tgcggccaaa agctatatcc tctaccggca gaagagggcg gagctgcgca
     1141 gtgcgcgcca ggccttggcg cgtcagatcg gggtcgaggg cctggaggga tgtctggcgg
     1201 agatacagac cgaatttgac tcggcccttt atcctctcac cgccctgtca acgcagttcg
     1261 cgggctttgc aaagcccggg atgacccagg cggaactatt ggcggtgctc accaaggcgg
     1321 cggtggagtt gaccagcccc gaggcgcccc agtgggagct gatcgccgcc cggtttttga
     1381 atttcagctt tcggctgcag ctggaggaag agctgctgcg ccgcggcatc ggcagctttt
     1441 atgaaaagct ggtctatctc acccgcgagg gcctgtacgg cgaatatatc ctgcaaaact
     1501 acaccaaagc ggaactggag gaggcggccg ggtttatccg gcagaagagg gacggccgct
     1561 tcacctggtc gggccttgat ctgctgctca agcgctatgt gatccgcacc cgccagcatg
     1621 tggcgctgga gacgccccag gagatgtttc tcggcatcgc gctgcatctg gccatgaatg
     1681 agaagcaggg gcgcatggcc tgggtgcggc gcttttacga catgctgagc ctgatgcagg
     1741 tcacaatggc gacgcccacc ctctccaacg cgcgcaagcc ctatcaccag ctctccagct
     1801 gctttatcga caccgtgcca gacagcctgg agggcatcta ccgcagcatc gacagctttg
     1861 ccaaggtgag caaattcggc ggcggcatgg ggctttattt cggcaaggtg cgcgccaccg
     1921 gcagcagcat ccgcggcttt gagggtgcgg cggggggcgt gatccgctgg atcaggctgg
     1981 tcaatgacac ggcggtggcg gtggaccagc tggggatgcg ccagggggcg gtggcggtct
     2041 acctggacgc ctggcacagg gacctgcctg agtttttaca gcttcgcacc aacaacggcg
     2101 acgaccgcat gaaagcgcac gacgtctttc cggccgtgtg ctggcccgac ctgttctgga
     2161 agcgggcgtc ccaagacctg gattccccct ggcatctgat gtgcccgcac gatattttga
     2221 ccgtcaaagg gtatgcgctg gaggattatt ggggcgagga atgggagcgg cgctacctca
     2281 gctgtgtcga ggacccccgc atccccaagc gcaccgtgac cgtcaaggag atcgtgcgcc
     2341 tggtgctgaa gagcgcagtg gagaccggca ccccgttcac gttcaaccgg gacgctgtaa
     2401 accgcgccaa tcccaacggc cacaagggca tgatctactg ctccaacctc tgcactgaga
     2461 tcgcccagaa catggccccg gtggagtcgg tgagcaccga ggtgacgacc cgcgagggcg
     2521 ataccgtggt ggtgaccacc acccggccgg gtgaatttgt ggtgtgcaat ctggcgagcc
     2581 tctccctggg caacctgccg gtagaggacg aagcggcgat gggcgaaatc gtgcgcacgg
     2641 cggtgcgggc gctggacaac gtgatcgatc tcaactttta cccgctgccc tatgccaggc
     2701 tcaccaacca gaaataccgc agcatcggcc tgggggtgag cggctaccac cacatgctgg
     2761 caaagcacgg cgttcgctgg gagagcgaag agcatctggc ctttgccgac cgggtgtttg
     2821 agaccatcaa ccgcgccgcc atcatcgcaa gctgcgagct ggcggaggaa aaaggcgctt
     2881 acagcctgtt tcccggcagc gattgggcca ccggcgccta ctttgaaaag cgggggtata
     2941 ccggccccgc gtggcgggcg ctggcagagc gggtgcggcg aagcgggatg cgcaacgcct
     3001 atctgctggc cgtggcgccc accagcagca ccagcatcct ggcgggcacc accgccggta
     3061 tagacccggt gatgaacaag ttctttctcg aggagaaaaa gggcagcatg ctgccccggg
     3121 tggcgccgga gctctcgcag gccacctatt ggtactacaa aaacgcccat ctcatcgacc
     3181 agcgctggag cgtgcgggcc tgcggggtgc gccagcgcca catcgaccaa gcccagagca
     3241 tgaacctgta tatcaccaac gagtacacgc tgcgccaggt gctggacctc tatctgctgg
     3301 cctggaagca gggcgtgaag acgatttatt atatccgcag caaatcactg gaggtggaag
     3361 agtgcgagag ct
//
LOCUS       DBMDYA010000274         3369 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6393_length_3369_cov_1.349755, whole genome shotgun sequence.
ACCESSION   DBMDYA010000274 DBMDYA010000000
VERSION     DBMDYA010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6393_length_3369_cov_1.349755"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="ACZPFK_07545"
     CDS             <1..479
                     /locus_tag="ACZPFK_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1406201.1"
                     /translation="VDLARQEGQAAARVRNVSARALDIPPEELVERFVRGDASRTDGG
                     SGLGLSIAKTFAEACGGVFELRFDADLVTAAVRLPLAPEDGEKRRGPTADAGASADAA
                     APAEAVRPALGGEPGASAAESGGTTKTPPPQGGDGAAETAGQPSAPQAAGAGELQP"
     gene            complement(470..652)
                     /locus_tag="ACZPFK_07550"
     CDS             complement(470..652)
                     /locus_tag="ACZPFK_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406202.1"
                     /translation="MFQVKKTEYSNKTFRLPCDLIADLEQLAQDKGVSLNQLVIQCCQ
                     YALDNLDRGEQPPHQG"
     gene            1034..1363
                     /locus_tag="ACZPFK_07555"
     CDS             1034..1363
                     /locus_tag="ACZPFK_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406203.1"
                     /translation="MRDGIERFVATARRIAVLLVILCAFLLLLASAPAAGAASAPVPA
                     SAPAQLVRWDGRWLENVSGGRCRAQVLMEGPQGPRSRVYLLAPGERKNVAGREGERAV
                     SVRAWAL"
     gene            2128..2610
                     /locus_tag="ACZPFK_07560"
     CDS             2128..2610
                     /locus_tag="ACZPFK_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1406204.1"
                     /translation="MAKGKGGNTVAVVERIARPVVEALGYRLWDVRYEKEGPDHFLRV
                     FIDKDGDMDTADCEKVSRALDPLIDEADPIEESYYFEVGSPGLGRRLTRPEHFAAVCG
                     EKVLVKLYRADASGQKEFTGVLTGAGEGQIALDTGEGERRFALSDVALAKLCDDEDLF
                     "
     gene            2794..>3369
                     /locus_tag="ACZPFK_07565"
     CDS             2794..>3369
                     /locus_tag="ACZPFK_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HIR1406205.1"
                     /translation="MNSEFFEALALLEKERGISEDYLVEKIQAAIVIAVKKSYDVEEN
                     VNVVIEPQTGKFSVAIVKDVVDEVEDPRTQMTLEEAQTVKKSARVGTQVEIPLKPKEF
                     GRIAAQTAKHVIRQALREAERNQMYAEMQSKAHEIVSAVVLKVDPVRGIVVVDLGKNE
                     AILPHNEQVPGEVLREGERVKVYVVDVAVTDK"
BASE COUNT          699 a          929 c         1143 g          598 t
ORIGIN      
        1 atgtggacct ggcccggcag gagggccagg cggcggcgcg ggtgcgcaac gtcagcgccc
       61 gcgcgctgga tatcccgccg gaagagctgg tggaacggtt cgtgcgcggg gacgccagcc
      121 gcaccgacgg cggcagcggg ctgggcctct ccatcgccaa gacctttgcg gaggcctgcg
      181 gcggcgtgtt cgagctgcgc tttgacgccg acctggtgac cgcggcggtg cggctgcccc
      241 tagccccgga ggacggggaa aaaaggcgcg ggcccaccgc ggacgccggc gcttctgcgg
      301 acgccgcggc cccggcggag gccgtgcggc ccgccctggg cggagagccg ggggcctctg
      361 ccgcggagag cggcggcaca acaaaaacgc cgcccccgca gggcggggac ggcgcggcgg
      421 aaacggcggg acagccctct gcgccgcagg cggcaggggc gggtgagctt caaccctgat
      481 ggggcggctg ctccccccgg tccagattgt ccagcgcgta ctgacagcac tggatcacga
      541 gctggttgag ggagacgccc ttgtcctggg cgagctgctc gagatcggca atgaggtcgc
      601 agggaagccg aaacgtcttg ttgctgtact cggtcttttt tacctgaaac atgacactca
      661 cctcctgact ctcattttag gtgtaaaata aaatattata tacacgctat atttacactt
      721 ataaatatat tgtaaaattc gatgcaatat gataggatgg agttaaaagc gaaaaagatc
      781 gacggcgcag tcgggtacgg tcaccggcgc tcggcaaaaa aagcttgatg tatcattgta
      841 ttaatgatat aatacaaaag ctttcaggtt tttttcaggt ttttctgtta catttaaaaa
      901 agaaaaccgt ctttataaaa aagagcggca tttttaaagc gctgggaaaa aggcaaactt
      961 gacagcaaag tgcgaaaaag cgtagaataa gagaaaactt acgattgtaa gctgtattgg
     1021 ggagggacgc gggatgagag acgggataga gcggtttgtg gcaacggcgc gccgcatcgc
     1081 cgtgctgctg gtgatcctgt gcgcctttct tcttctgctg gcttcggcgc cggccgcggg
     1141 ggcggcttcc gccccagtcc ctgcttccgc cccggcgcag ctggtccgct gggacggccg
     1201 ctggcttgag aacgtgagcg gcggccgctg ccgggcgcag gtgctgatgg agggcccgca
     1261 gggcccgcgc agccgcgtct atctgttggc gcccggggag cggaaaaacg tggcgggcag
     1321 agagggcgag cgcgccgtga gcgtccgcgc ctgggcgctc tgagccgctc gcggcgcggg
     1381 ccccgcgcag acggccctag acgcggaaga gccgggccgg gaagttctgt gcggatgcgc
     1441 acaggcctca agccccctgt tgccgacaag atgccgcttg cagcaagttt tttgcgcgag
     1501 cggctttata gacgataggt tagaataaaa ctctatgccg gttccgtgga agttttgcac
     1561 ggacccccac aaaaacagca gattgcgtgt aaagctggat gccgctttca gtggaagctc
     1621 cgcaaggcga gaacggcaag gccgttctcg tatggacggc cacaaagata taggttatgg
     1681 gtaaaaccat atgccgtttc cagtggaagt ttcgcttggc gagaacggca aggccgttct
     1741 cgtatggacg gccacaaaga ctataggtta tgggtaaaac tatatgccgc ttccagtgga
     1801 agtttcgcaa ggcgagaacg gcaaggccgt tctcgtatgg acggccacaa agatataggt
     1861 tatgggtaaa actatatgcc gcttccagtg gaagtttcgc ttggcgagaa cggcaaggcc
     1921 gttctcgtat ggacggccgc aaagacgata ggttatgggt aaaactgaaa gccgtcctaa
     1981 tgcttgcata tttccgccgc ttgtggtata gtatgctagt aagaagaata tcaaccgtga
     2041 agcgtgggcc gcgaggcccg cgcttcatgt ttgtaacggc ggttttgggc ggggcatccc
     2101 cgcgggccgc ctgctttgga ggtgcgtatg gcaaagggca agggcggcaa cacggtggcc
     2161 gtcgtcgagc gcattgcccg cccggtggtc gaagcgctcg gctaccggct gtgggatgtg
     2221 cgctacgaaa aagaggggcc ggatcatttt ttgcgggtct tcatcgacaa ggatggggat
     2281 atggacaccg cggactgcga aaaggtctcc cgggcgctgg acccgctgat cgacgaggcg
     2341 gaccccattg aggagagcta ttacttcgag gtcggcagcc cgggcctggg gcgcaggctg
     2401 acccggccgg agcacttcgc cgcggtctgc ggcgagaagg tgctggtaaa gctctaccgc
     2461 gccgacgcct ccgggcaaaa agaattcacc ggcgtcctca cgggcgcggg ggaggggcag
     2521 atcgccctgg acactgggga gggggagcgc cgcttcgccc tctccgacgt cgcgctggca
     2581 aagctctgcg acgatgagga ccttttttaa gccggcgcgg cccgtgcggc ccgccgttct
     2641 gccgtaaaag aggcttgggg ccgctcttgc gcgccctgtg tcccgccttt tgccgcgggg
     2701 ccgtcttggg ggccgccagc cccaggccgt cttttaccgc aaagccataa aaaacgcccg
     2761 cagcgccgcg ggtcagggag gaaaacagaa aacatgaaca gcgaattttt cgaggctctt
     2821 gcccttctgg agaaagagcg cggcatcagc gaggattacc tggtggagaa gatccaggcc
     2881 gccatcgtca tcgcggtcaa aaagagctat gatgtagagg agaacgtcaa cgtcgtcatc
     2941 gagccccaga ccggcaagtt ctcggtggcc atcgtcaagg acgtggtgga cgaggtggag
     3001 gatccgcgca ctcagatgac gctggaggaa gcgcagaccg tcaaaaagag cgcccgcgtg
     3061 ggcacccagg tggagatccc cctcaagccg aaagagtttg gccgcatcgc ggcgcagacg
     3121 gcaaagcacg tcatccgcca ggccctgcgc gaggccgagc gcaaccagat gtacgccgag
     3181 atgcagagca aggcgcacga gatcgtctcg gccgtggtgc tcaaggtcga cccggtgcgc
     3241 ggcatcgtgg tggtggacct gggcaaaaac gaggccatcc tgccccacaa cgagcaggtg
     3301 cccggcgagg tgctgcgcga gggcgagcgg gtcaaggtct atgttgtgga tgtcgcggtc
     3361 accgacaag
//
LOCUS       DBMDYA010000275         3363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6405_length_3363_cov_1.550582, whole genome shotgun sequence.
ACCESSION   DBMDYA010000275 DBMDYA010000000
VERSION     DBMDYA010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_3363_cov_1.550582"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(80..763)
                     /locus_tag="ACZPFK_07570"
     CDS             complement(80..763)
                     /locus_tag="ACZPFK_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015820.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HIR1406206.1"
                     /translation="MIAVISPAKNMRRAPSPLPPSEPQFLPQARRLLAELRGYSPWRL
                     AHEMRINDGLAQRAFTDFADMDLSQPGTPALLSYHGLQYLNLGPETLSAQELLFLQEH
                     LRIVSGFYGLLRPLDGIWPYRLELGGKLRFFGQTLYGYWGDAIYRALFASAETVVNLS
                     SGEYTKAFTPYLCAEDAFITVDFLEPKPGGKLVMRATAAKMARGRMARFIARRRVDDP
                     QELKEFCWA"
     gene            complement(760..1224)
                     /gene="bcp"
                     /locus_tag="ACZPFK_07575"
     CDS             complement(760..1224)
                     /gene="bcp"
                     /locus_tag="ACZPFK_07575"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR1406207.1"
                     /translation="MLKAGEKAPDFTLPDKDGNPVSLAAFRGKKVVVYFYPKDNTPGC
                     TRQACAFRDAYEGFRSADVAVIGISKDSEGSHQKFAEKFDLPFLLVSDPERTVIEAFG
                     VWQEKKLYGKVSMGVVRATFIIDESGVIEKVFPRAKPDTNAAEILAYLGKQP"
     gene            1728..2096
                     /locus_tag="ACZPFK_07580"
     CDS             1728..2096
                     /locus_tag="ACZPFK_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1406208.1"
                     /translation="MLDKNGIERCDYIHAHPGIIQAAQAAMPDEELLYDLAELYKVFG
                     DSTRIKILYVLFEAEMCVCDIAQLLGMTQSAISHQLRVLKGSKLVKNRRDGKTVFYSL
                     ADDHVRTILGQGMDHITEED"
     gene            2111..2329
                     /locus_tag="ACZPFK_07585"
     CDS             2111..2329
                     /locus_tag="ACZPFK_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1406209.1"
                     /translation="MKKTFKLQDLDCANCAAKMETAIKKLDGVTDASVSFMTQKLVLE
                     ADDARFDAILKEVVKACKKVEPDCTILL"
     gene            2483..>3363
                     /locus_tag="ACZPFK_07590"
     CDS             2483..>3363
                     /locus_tag="ACZPFK_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1406210.1"
                     /translation="MTRKQKKALIRILISAALFLAGELVPLEGWWKLVFFLPAYLIVG
                     GDVLLKAARNIAHGQIFDENFLMCIATVGAFGLALYDQITGAESMVGYDEAVAVMLFY
                     QVGELFQSYAVGKSRASIASLMDIRPDYANIRRDGTLVQVDPEEVAIGDVIVVKAGEK
                     IPLDGTVLSGETTVDTAALTGESVPRDLGPGGEAVSGCINLSGLIEVRVEKPFGESTV
                     AKILDLVENASSKKAKAENFITKFARYYTPAVVIAAVLLAVAPPLFLGGWGEWIHRAL
                     IFLVVSCPCALVISIPL"
BASE COUNT          672 a          958 c         1079 g          654 t
ORIGIN      
        1 agccgcttga tgtggttctg cagcggctgc gcgttcttat tgtagtcgtc cttcaggctg
       61 tcgatccggc gatccagttc tacgcccagc agaactcttt cagctcttgc gggtcatcga
      121 cgcgccgccg ggcgataaag cgggccatcc gcccacgggc cattttggcc gcggtggccc
      181 gcatcaccag cttgccgccg ggcttcggct ccaaaaaatc caccgtgatg aaagcgtcct
      241 ccgcgcacag atagggggtg aacgccttgg tgtattcccc ggagctcagg ttgaccaccg
      301 tctcggcgga ggcaaacagc gcccggtaga tggcgtctcc ccaatagccg taaagggtct
      361 ggccgaaaaa gcgcagcttt ccccccagct ccaggcggta gggccagatg ccgtccagcg
      421 gccgcagcag gccgtaaaag ccgctcacaa tgcgcagatg ctcctgcaaa aacaacagct
      481 cctgcgccga cagggtctcc ggccccaggt tcaggtattg cagcccatgg taggacagca
      541 gggcaggggt gcccggctgc gagagatcca tatccgcaaa gtcggtgaaa gcgcgctggg
      601 ccagcccgtc gttgatgcgc atctcatgcg ccaaccgcca ggggctgtag ccgcgcagct
      661 cggccagcag acgccgcgcc tggggcagaa actgcggctc gctggggggc agcgggctgg
      721 gggcgcggcg catgttcttt gcgggggaga tgaccgcgat cacggctgct tgccgagata
      781 ggccaggatc tcggcggcgt tggtgtcggg cttggcgcgc gggaacacct tttcgatcac
      841 gccgctctca tcgatgataa aggtggcgcg caccacaccc atcgagacct tgccgtaaag
      901 ctttttctcc tgccagacgc cgaacgcctc gatgacggtg cgctccgggt cgctcaccag
      961 caaaaacggc agatcgaact tttcggcaaa tttctgatgg ctgccctcgc tgtccttgct
     1021 gataccgatg acggccacgt cggcagagcg gaagccctcg taggcgtccc gaaaggcgca
     1081 ggcctggcga gtgcagcccg gggtgttgtc cttgggataa aagtagacga ccactttttt
     1141 gccccgaaac gcggccaggc tgaccgggtt gccgtccttg tcgggcagag tgaaatcggg
     1201 cgctttttct ccggccttga gcatgttgaa aacctccgtt gtcacaattt ttttctgatc
     1261 cttttgtttc aggataacac aaaaaacaca aaaagtctac taaaaaagta ttgtttcaaa
     1321 acgccgcttt gagccggtca gagggagggc ggagtgggga gcgccttgcc cgacagggcg
     1381 aaaccgcgcg gtggcgtcga ggtgcggaaa gggggttctt tggcagggaa gcggagcgcc
     1441 cgcgggcgga tagggcttgt aagcggcgtt gcggcgctgg gcggcctgtt tcgacgcgat
     1501 aagaccctta tctgtccgtt taaaattgcg tgaaaggcgc gctttccccc cttttgaaac
     1561 ggaaaacaag agctcccggg cgtttggcga gggcggcggg ccgcttttgc cgctttttat
     1621 tttgccggtt tttgaaaatt tcatccgcgc ccacttgaca acggcggata gcttgctata
     1681 atgaatacat gaacagttgt tcatatgttt taaaaggagg cgccgtcatg ctggacaaaa
     1741 acgggattga acgctgcgac tacatccacg cccacccggg catcattcag gccgcgcagg
     1801 ccgccatgcc ggatgaagag ctgctctatg accttgccga gctgtacaag gtcttcggcg
     1861 actccacccg aatcaagatc ctctatgtgc tgttcgaggc ggagatgtgc gtgtgcgata
     1921 tcgcgcagct gctggggatg acccaatccg ccatttccca tcagctgagg gtgctcaagg
     1981 gctcaaagct ggtcaaaaac cgacgggacg gcaagacggt gttttattcc ctggcggacg
     2041 accacgtgcg caccatcctc gggcagggca tggaccatat cacagaagaa gattgaagga
     2101 gtgttgtagt atgaaaaaga cctttaagct gcaggacctg gactgcgcca actgcgcggc
     2161 caagatggag accgccatca aaaagctgga cggcgtgacg gacgccagcg tgagcttcat
     2221 gacccaaaag ctggtgctgg aggcggacga cgcccgcttt gacgccatcc tgaaagaggt
     2281 cgtcaaggcc tgcaaaaagg tggagccgga ctgcaccatt ttgctgtaaa gagcctttgc
     2341 cgctgtcctg agccgaggtt ttgaaaagag gtttttaaaa aatgaaaaaa cgcggcggcc
     2401 ccctgggcgg ccgcgcggag aaagaaagcc ggtgcgggca ggcaggtctt gcgcccgcag
     2461 tggagagagg ggaaatagtc cgatgaccag aaagcagaaa aaggcattga tccgcatcct
     2521 gatcagcgcg gcgctttttc tggcggggga gctggtgccg ctggagggct ggtggaagct
     2581 ggtcttcttt ctgcccgcgt acctcattgt gggcggcgac gtgctgctga aagccgcaag
     2641 gaacattgcc cacggccaga ttttcgatga aaattttctc atgtgcatcg ccacggtggg
     2701 ggcgttcggg ctggcgctgt acgatcagat caccggcgcc gagtcgatgg tgggctatga
     2761 cgaagcggtc gccgtcatgc tgttttatca ggtgggcgag ctgtttcaga gctacgcggt
     2821 gggcaagtcc cgcgcctcca tcgccagcct catggatatc cggccggatt acgccaacat
     2881 ccgccgggac ggcacgctgg tgcaggtgga ccccgaggag gtcgccatcg gcgacgtgat
     2941 cgtggtcaag gcgggggaga agatcccgct ggacggcacc gtgctgtcgg gtgagaccac
     3001 agtggatacc gcggccctca ccggcgagtc ggtgccccgc gacctggggc cgggcggcga
     3061 ggcggtcagc ggctgcatca acctcagcgg cctcatcgag gtgcgggtcg aaaagccgtt
     3121 tggtgagtcg acggtggcaa agatcctcga cctggtcgag aacgccagca gcaaaaaggc
     3181 gaaagcggaa aatttcatca caaaattcgc ccgctactac acccccgccg tggtcatcgc
     3241 ggctgtgctg ctggccgtgg cgccgcccct gtttttgggc ggctggggcg agtggattca
     3301 ccgggcgctg atctttttgg tggtgtcctg cccctgcgcg ctggtcatct cgatccccct
     3361 cag
//
LOCUS       DBMDYA010000276         3356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6424_length_3356_cov_1.285100, whole genome shotgun sequence.
ACCESSION   DBMDYA010000276 DBMDYA010000000
VERSION     DBMDYA010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6424_length_3356_cov_1.285100"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="ACZPFK_07595"
     CDS             <1..320
                     /locus_tag="ACZPFK_07595"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1406211.1"
                     /translation="DFGVGYSGEGILVDLAPQFVKIDISLVQGIERDEDRQALLSNLV
                     LYCKKRRIRLIGEGVETFAEMEYLIRAGVDYLQGYYLCRPAHTPPERLPCKKEILAIR
                     SKM"
     gene            999..2054
                     /locus_tag="ACZPFK_07600"
     CDS             999..2054
                     /locus_tag="ACZPFK_07600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406212.1"
                     /translation="MDNNTIVALKDIVVDFDGEAVLTGLSLDIHDKEFVTLLGPSGCG
                     KTTTLRVIGGFLEPKSGSVFFNGKDITRLPPYKRQVNTVFQKYALFPHLNVYENVAFG
                     LRLRKVEEKELKARVLDMLELVGLRGFERRDPTTLSGGQQQRVAIARALVNHPKVLLL
                     DEPLGALDLKLRKEMQTELKRIQQSLDITFIYVTHDQEEALTMSDTVVVMNGGYIQQI
                     GSPVDIYNEPQNAFVADFIGESNILSGVMERDFSVEFAGCQFTCVDKGFAPRALVDVV
                     VRPEDIQVVPAIQGKLSGVVKSVIFKGVHYEILVEQAGTEWKIHSTQAQQPGEIIGMN
                     IGPDEIHIMHKMEEAAR"
     gene            2051..2869
                     /locus_tag="ACZPFK_07605"
     CDS             2051..2869
                     /locus_tag="ACZPFK_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406213.1"
                     /translation="MKLKSKACAAPYLVWMVAFIVAPLLMVIYFAFTDKSGKFTLENL
                     SGLSQYAPVFMRSILLAAIATVICLILAYPLSFMLSRLRANKQRIMLMLVMLPMWMNF
                     LLRTYAWMTLLERNGLINKFLGLFGLGPFNMINTQGAVVLGMVYNYLPYMILPLYTIM
                     VKIDDSLIEAAEDLGAGLFHKVTKVLIPLSLPGITTGITMVFVPSISTFIISRMLGGG
                     SNLLIGDLIELQFLGNSYNYNLGSAMSLVLMVLVLLCMSLTNMMDSESKEGGVL"
     gene            2869..>3356
                     /locus_tag="ACZPFK_07610"
     CDS             2869..>3356
                     /locus_tag="ACZPFK_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406214.1"
                     /translation="MKKMGAFSKFYMILIFSFMYVPIAVMAVFSFNQSKSRTLFTGFT
                     LDWYRNLFRNEMILSALGISLVIALVASVVATVLGTMASIGIHSMGRTARGLIMNVSY
                     MPVINPEIITGVSMMLLFVVFKGWMGGKVFGMPTLLIAHITFCVPYVIFNVSPKLRQM
                     DVR"
BASE COUNT          691 a          916 c          989 g          760 t
ORIGIN      
        1 acgatttcgg cgtgggatac agcggcgagg gcatcctggt cgatctggcc ccgcagttcg
       61 tcaagatcga tatttccctg gtacagggca tcgagcggga tgaagaccgt caggctctgc
      121 tctccaatct ggtgctctac tgcaagaagc gccgcatccg cctgatcggg gagggcgtgg
      181 agacctttgc cgagatggag tacctgatcc gcgcgggcgt ggactatctg cagggctatt
      241 atctctgcag gcccgcccac acgccgccgg agcgcctgcc ctgcaaaaag gagattctgg
      301 ccattcggag caagatgtaa agggcgcggg gatgaataaa aagtagccgc tttgcggcta
      361 tttttttgac catccgtttt gattgccgcg gcagcggcga gaaacggggc cgtcgagata
      421 agttgttttt tattgcgaag caataaaaaa tagccgcagg ctgtttttcg gccatccggt
      481 cagtcctctg gccggggccg tcgagataaa tcgttgttta ttgcagcgcg ataaacaaca
      541 gaaaaaagcg gggttgtccc cgctttttct tttgggaaag gcgcttgctt ttggcctttc
      601 ccatgccgca agaaaaaacg tgccggacaa agaaaaaatg aggttttggg agaaaatcgg
      661 aggatttccg gcatcctcta caaaaaatct tgattctttg cggtcttttc caaaaaatcg
      721 agaaatttgc gtaaaactat tgacaaaccc tgcgcccgcc tttatagtac atattgtgtg
      781 tttgcatcta aggtgcatgg tggggcggtc tgttccattc cgtgcgccgg gggcacggcg
      841 ctttccaccc gccggcccat cgcaacatgg gcgttgtatg tactgctgta gccgccgctg
      901 actgcggggg tgtaacaaac cttttgcggg gtagtataat tggcgcctgc ctggcaggcc
      961 ggggcctcta ggaccaatac cgcaaagggg gatgcaggat ggataataat acgatcgttg
     1021 cactgaaaga cattgttgta gattttgacg gggaagcggt ccttaccggg ttgtcgctgg
     1081 acattcacga taaggaattc gtcaccttgc tgggtccttc gggctgtggc aagacgacca
     1141 ctctgagggt gatcggcggc ttccttgagc caaagtcggg cagtgtcttt tttaatggca
     1201 aggacatcac gaggctgccg ccgtacaagc ggcaggtaaa cacggtcttt caaaaatacg
     1261 cgctgtttcc gcacctgaac gtctacgaaa acgtggcgtt cgggctgcgg ctgcgcaagg
     1321 tggaagaaaa agagctgaaa gcccgggtac tcgacatgct ggagctggtg ggcctgcgcg
     1381 gttttgagcg ccgcgacccg accactctct cgggcgggca gcagcagcgg gtggccatcg
     1441 cgcgggcgct ggtcaaccat cccaaggtgc tgctgctgga cgagcctctg ggcgcgctgg
     1501 atctcaagct gcgcaaggag atgcagaccg agctcaagcg catccagcag agcctggaca
     1561 tcacctttat ctatgtcacc catgaccagg aggaagccct gaccatgagc gataccgtcg
     1621 tggtcatgaa cggcggctat atccagcaga tcggcagccc ggtggatatc tataacgagc
     1681 cgcagaatgc attcgtagcg gattttatcg gcgagtcgaa catcctgtcc ggcgtgatgg
     1741 agcgcgattt cagcgtggag ttcgccggct gccagttcac ctgcgtggac aagggctttg
     1801 cgccgcgcgc cctggtggat gtggtggtgc ggcccgagga catccaggtc gtgcccgcca
     1861 tccagggaaa gctgtcgggc gtggtcaaaa gcgttatctt caagggcgta cattatgaaa
     1921 ttctcgtcga gcaggcgggc accgagtgga agatccattc cacccaggct cagcagccgg
     1981 gcgagatcat cggcatgaac atcgggccgg acgagatcca catcatgcac aagatggagg
     2041 aagcggcgcg atgaaattaa aatccaaagc ttgcgccgcg ccgtatctcg tctggatggt
     2101 ggcgtttatc gtcgcgcctc tgttgatggt catctatttt gcctttaccg acaaaagcgg
     2161 aaaattcacc ctggagaacc tctccgggct cagccagtac gcgccggtct ttatgcgcag
     2221 catcctgctg gcagccatcg ctacggtgat ctgcctcatc ctggcctatc cgctcagctt
     2281 catgctctcc cgcctgcggg ccaacaagca gcgcatcatg ctgatgctgg tgatgctgcc
     2341 catgtggatg aactttttgc tgcgcaccta tgcctggatg actctgctgg agcgcaacgg
     2401 cctgatcaac aaatttttgg gcctgttcgg cctggggcct ttcaatatga tcaacaccca
     2461 gggcgccgtg gtgctgggca tggtctataa ctatctgccc tatatgatcc tgccgctcta
     2521 caccatcatg gtcaaaatcg acgacagcct gatcgaggcg gcggaggacc tgggcgccgg
     2581 cctgttccac aaggtgacca aggtgctgat cccgctctca ctgccgggca tcaccaccgg
     2641 catcaccatg gtctttgtgc ccagcatctc cacttttatc atcagccgta tgctgggcgg
     2701 cggctccaat ctgctcatcg gcgacctgat cgagctgcag ttcctcggca acagctacaa
     2761 ctataacctg ggcagcgcaa tgagcctggt gttgatggtg ctggtgctgc tgtgcatgag
     2821 cctgaccaat atgatggaca gcgagagcaa ggaagggggc gttctctgat gaaaaaaatg
     2881 ggggctttct ccaagtttta catgatcctc atcttctctt ttatgtatgt gccgattgcg
     2941 gtgatggcgg tgttcagctt caaccagtcc aagagccgca cccttttcac aggctttacg
     3001 ctggactggt accggaatct gttccgcaat gagatgatcc tcagcgcgct gggcatctcg
     3061 ctggtcattg cgctggtggc ttcggtggtc gccaccgtgc tgggcaccat ggccagcatc
     3121 ggtatccatt ccatgggccg caccgcgcgc gggctcatca tgaacgtcag ctacatgccg
     3181 gtcatcaacc ccgagatcat caccggcgtc tcgatgatgc tgttgttcgt ggtcttcaag
     3241 ggctggatgg gcggcaaggt gttcggcatg ccgacgctgc tcatcgccca catcaccttt
     3301 tgcgtgccct atgtcatctt caatgtgtcg cccaagctgc gccagatgga cgtgcg
//
LOCUS       DBMDYA010000277         3350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6436_length_3350_cov_1.563558, whole genome shotgun sequence.
ACCESSION   DBMDYA010000277 DBMDYA010000000
VERSION     DBMDYA010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6436_length_3350_cov_1.563558"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="ACZPFK_07615"
     CDS             complement(<1..478)
                     /locus_tag="ACZPFK_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1406215.1"
                     /translation="MSWDSGVLAPEERASLALRGLFEQYGYRKYRMGQFEEYSLYMDN
                     KSFLPSENVIAFTDLDGRLMALKPDVTLSIVRHAAERPATAEKLYYIESVYRPSAESH
                     TFKEISQMGVEYIGRVDRYAMAEVAALAAKSLAEVGDDYLLEIGHMRFTAALLDALG"
     gene            complement(941..2119)
                     /locus_tag="ACZPFK_07620"
     CDS             complement(941..2119)
                     /locus_tag="ACZPFK_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1406216.1"
                     /translation="MKLDKKTLQRLGLGLAGLILFAWCLDNNKVVFRLLGTLVGMLAP
                     FLIGLVIAFILNVPMRALERGLFRGARARGGRAAGAARMASFALTLVLLLGGAGLISF
                     IVIPEMGNTFALLGRQMPAFLQRVQQQAKALEAWLPQVEDLVQSLELDWKSIAESAMG
                     FLQSGATSILGSTFSIATSVFNGVFNFFMGFIFAVYILFRKEHLAVQARRMLYAWMPV
                     PRADRIIYVASMTERTFSKFLTGQCLEAMILGLMFFVSMTLLRFPYAMLVAVLVAVTA
                     LSPMFGAFIGCFVGAFLILVVSPIQALWFVLLFMVLQQIEGNLIYPRVVGSSIGLPAM
                     WVMVAVTLGGSTMGVLGMLIMVPAASVIYALARENTEHRLLRRQVPADRYAESSRKEK
                     "
     gene            2106..2444
                     /locus_tag="ACZPFK_07625"
     CDS             2106..2444
                     /locus_tag="ACZPFK_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406217.1"
                     /translation="MSNFIFTSKASGPTLHPGLFRGPRAVLLSILTHFPLWHNPRGKT
                     TYLERPVSRRCAATETDGHKNSRAAAIFLLLRNKKLLILMAPFLAAARQPKRMAQKIA
                     ALRLFFTEPP"
     gene            2496..3269
                     /locus_tag="ACZPFK_07630"
     CDS             2496..3269
                     /locus_tag="ACZPFK_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1406218.1"
                     /translation="MKDNGEYTVKALAQLAGVSSRTLRYYDRIGLLHPARTSGSGYRL
                     YGAAEVDRLQQILFYRELELPLAQIRAILSSPDFDRRAALGQHLAALESRRQRLDRLI
                     ETARATLAADKGGTKMTDREKFEGFKQEQLAHNEAAHGKETRQRWGDEAVDASNRRFA
                     GLSQEEYDAMQALAEEILTRLEAAVAAGADPAGEEGRAIAQLHRQWLGYTWPSYSAPA
                     HLGLAEMYVADERFTAYYDARRPGCAAFLRDAIAALVHG"
BASE COUNT          685 a         1107 c          979 g          579 t
ORIGIN      
        1 gccccagcgc gtccagcagg gcggcggtaa agcgcatatg gccgatctcc agcagatagt
       61 cgtcccccac ctccgccagg cttttggccg ccagcgccgc cacctcggcc atggcatagc
      121 ggtccaccct gccgatgtac tccaccccca tctggctgat ctctttaaag gtgtggctct
      181 ccgcgctggg gcggtagacg ctttcgatgt aatagagctt ttccgccgtg gccggccgct
      241 cggccgcatg gcgcacgatg ctcagcgtca catcgggctt gagggccatc agccgcccgt
      301 ccaggtcggt gaaagcgatg acgttctcgc tgggcaaaaa gctcttgttg tccatataaa
      361 ggctgtattc ctcaaactgg cccatccggt atttgcggta cccgtactgc tcgaacaggc
      421 cccgaagcgc cagactggca cgttcctcag gggcaagcac gccgctgtcc caggacatga
      481 aacccctcct ctccattttg ctttattgtg ctatagtgat aacgtgataa attaataaag
      541 caattgtacc acagaggcgg gcagatttcc actgtttttt tgccggttta caggcgcggg
      601 gacaggcctt ttttcgtgag gtgccacaaa acgcgcctct aacggctgct cttttgcagt
      661 gtgccagagc ccggcctcat ccatttgcgg gcgcaggtta aaagggcgtc aggccggggc
      721 cgaaaaggcc gcccgggctc tcctccgctc gtgggcgctg gacaaaggaa cgcttttgcc
      781 ctgcgtccga cggcggggcg gacgtgcttt ccgcggggca ataaaaagcc ggggacaggc
      841 cggaggtctc cggcccgtcc ccgggcaatt gatctgaaag cggttccccc tcccgcctgc
      901 acgagctact ccggcgggag aacctgctga agcgggcgcg ctacttctct ttgcggctgc
      961 tttcagcata gcggtcggcg ggcacctggc gccgcagcag ccggtgctcg gtattctccc
     1021 tcgccagcgc gtagatcacc gaggccgccg gcaccatgat cagcatcccc agcaccccca
     1081 tggtgctgcc gcccagcgtg accgccacca tgacccacat ggcgggcagc ccgatggagc
     1141 tgccgaccac gcgcgggtag atgagattgc cctcgatctg ctgcagcacc atgaaaagca
     1201 gcacgaacca cagcgcctgg atggggctga ccaccaggat caggaacgcg cccacaaagc
     1261 agccgatgaa agcgccgaac atggggctca gcgccgtgac ggccaccagc accgccacca
     1321 gcatggcata ggggaaacgc agcagcgtca tgctgacaaa gaacatcagc cccaggatca
     1381 tggcctccag gcactgcccg gtcaaaaact tggaaaaggt gcgctcggtc atcgaggcca
     1441 cataaattat ccggtcggca cgcggcaccg gcatccaggc gtacagcatg cgccgggcct
     1501 gcaccgccag atgctctttg cggaacagga tatagactgc gaagatgaaa cccatgaaaa
     1561 agttgaacac accgttgaaa acagaggttg caatactgaa tgtagacccc aggatagagg
     1621 tcgcgccgct ctgcaaaaag cccatcgccg actcggcgat gcttttccag tccagctcca
     1681 gcgactgcac caggtcctcc acctggggca gccaggcctc cagcgccttt gcctgctgct
     1741 gtacccgctg caaaaaggcg ggcatctgcc tgcccagcag cgcaaaggtg ttgcccatct
     1801 cggggatgac gataaacgag atcaggcccg caccgcccag cagcagcacc agcgtcagcg
     1861 cgaagctcgc catgcgggcc gcccccgcgg cccggcctcc ccgcgcccgc gccccgcgaa
     1921 acagcccgcg ctccagcgcg cgcatgggca cattcaaaat aaaggcgatc accaagccga
     1981 tcaaaaacgg ggccagcatg cccaccagcg ttcccagcag ccggaacacg accttgttgt
     2041 tgtccaggca ccaggcaaac aggatcagcc ccgccagccc aaggcccaag cgctgcagcg
     2101 tctttttgtc caatttcatt tttacctcca aggcgtccgg gcccacactg catccaggtc
     2161 tctttcgcgg gccgcgcgcc gtcctgctct ctattctaac gcatttcccg ctgtggcaca
     2221 atccgcgcgg caaaacaact tatcttgagc gccccgtttc ccgccgctgc gcggcaaccg
     2281 aaaccgacgg ccacaaaaat agccgcgctg cggctatttt tttattgctt cgcaataaaa
     2341 aacttcttat cttgatggcc ccgtttctcg ccgctgcgcg gcaaccgaaa cggatggccc
     2401 aaaaaatagc cgcactgcgg ctatttttta ccgaaccccc ttgaccttga cgcagcgtca
     2461 tcccttatgc taaggtcatg gagggaggga cagccatgaa agacaacgga gaatacacgg
     2521 taaaagcgct ggcgcagctc gccggcgtga gcagccgcac gctgcgttac tacgaccgca
     2581 tcggtctgct gcatccggcc cggaccagcg gatccggcta ccggctctac ggcgccgccg
     2641 aggtggaccg gctgcagcag atcctgttct atcgcgagct ggagctgccg ctcgcacaga
     2701 tcagggccat cctctcctct ccggattttg accgccgcgc ggcgctgggg cagcatctgg
     2761 cggcgctcga gagccgccgg cagcgcctgg accggctgat cgagacagcc cgcgcaacac
     2821 tggcggcaga caagggaggc acaaaaatga cagatcgaga aaagttcgag ggcttcaagc
     2881 aggagcagct tgcccacaat gaggcggctc acggcaaaga aaccagacag cgctggggag
     2941 atgaggctgt ggacgcttca aaccgcaggt tcgcaggcct ttcgcaagag gaatacgacg
     3001 cgatgcaggc cttggcagag gagattctca cgcggctgga ggcggcggtc gcggcggggg
     3061 cggaccccgc tggagaagag ggacgcgcca tcgcccagct gcaccggcaa tggctgggct
     3121 acacctggcc gtcctattcc gcccccgcgc atttggggct cgcggagatg tatgttgccg
     3181 atgagcgctt caccgcctat tacgacgccc gccgccccgg ctgcgcggcc tttttgcggg
     3241 acgcaatcgc ggccctggtg catggctgag actttctcgc gccgggccgc tctcgggcat
     3301 cggggttgaa cagctaaaaa atagccgcaa cgcggctatt ttttgaccat
//
LOCUS       DBMDYA010000278         3342 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6444_length_3342_cov_1.528541, whole genome shotgun sequence.
ACCESSION   DBMDYA010000278 DBMDYA010000000
VERSION     DBMDYA010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6444_length_3342_cov_1.528541"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="ACZPFK_07635"
     CDS             complement(<1..589)
                     /locus_tag="ACZPFK_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1406219.1"
                     /translation="MKTKKEVLEIIGRLKGAYPLAECTLDYDHAWQLLVSVRLAAQCT
                     DARVNIVTEELFAKYPSVAALAEADPADIEAIVKPCGLGHSKARDISLAMRKLMSDFG
                     GRVPDDFDTLLTLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLCNKMGIVKDLKEPKK
                     VEMALWKVIPPEEGNDFCHRLVLHGRAVCSARSPRC"
     gene            901..2151
                     /locus_tag="ACZPFK_07640"
     CDS             901..2151
                     /locus_tag="ACZPFK_07640"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1406220.1"
                     /translation="MFGDVLDTIGFVKERDPEVGAAMEREMGRLRGNIELIASENVVS
                     PAVMAAMGSILTNKYAEGYPGHRYYGGCQFVDEVEELARNRACELFGADHANVQPHSG
                     AQANLAVYFALLQPGDTVLGMNLSHGGHLTHGSPVNMSGKYFRFVPYGVDADGFLDYD
                     ALRELALAEKPKLIVAGASAYARTIDFARISEIAKEAGCLFMVDMAHIAGLVAAGLHP
                     SPVPYADVVTTTTHKTLRGPRGGMILCKEQYAKAIDKAIFPGTQGGPLMHVIAGKAVC
                     FGEALTPEFKEYQRRIVENAKALSEALLAKGVHLVSGGTDNHLMTVDLRGTGVTGKQL
                     EARLDEVNITANKNTVPNDPEKPTVTSGVRIGTPAVTTRGFGVAEMEEIAGFIAGCVF
                     DFEGKKEEIKAGVAALTARFPLYE"
     gene            2363..3199
                     /locus_tag="ACZPFK_07645"
     CDS             2363..3199
                     /locus_tag="ACZPFK_07645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1406221.1"
                     /translation="MAYINAKLRSQVLLRTVDVALYYPNDYPEEVVPGIRGVFTLLHG
                     FNGCGQDWLQLSAATRYAADNGLILVAPSCENSFYCDMVYGDAWYTFLTDELPLLLEK
                     FFRVPTAREQNFIAGLSMGGYGAMMLAMTHPERYAGAASFSGAVDLSLMLQQGAQLPE
                     LRKSFVPIFGEQLALPQDRDLRVLAGRLSGLPKERQPKLLFTCGLEDNEPYYIHLQNR
                     SLYDSIHTLPFAAYRYMEWPGNHEWKFWDRSLVYAIDWFLSPGYAEAKHGDWRSEAQI
                     IP"
BASE COUNT          609 a         1061 c         1086 g          586 t
ORIGIN      
        1 cgcagcgggg gctgcgggcg ctgcacacgg cgcgcccgtg caggaccagc cggtggcaaa
       61 agtcattgcc ctcctcgggc ggaatgactt tccacagcgc catctccacc tttttcggct
      121 ctttcaggtc cttgacgatc cccatcttgt tgcacaggcg gatgcagtgg gtatcggtga
      181 cgatggcggg cttgccgaac acgtccccca tgatcaggtt ggcgcttttg cgtcccacgc
      241 ccggcagggt gagcagggtg tcgaagtcgt ccggcaccct gccgccgaaa tcgctcatca
      301 gcttgcgcat ggcaaggctg atgtcccggg ccttgctgtg gcccaggccg cagggcttga
      361 cgatggcctc gatgtcggcc gggtccgcct cggccagggc cgccaccgac gggtattttg
      421 caaacagctc ttcggtgacg atgttcaccc gcgcgtcggt gcactgggcg gccagccgca
      481 cgctcaccag cagctgccag gcgtggtcat agtccagggt gcactcggcc aggggatatg
      541 ctcctttcaa ccgaccaatg atctcgagca cctctttttt tgtcttcaaa ccggactcct
      601 cctccgaggc gccgccctgt gccggcgcgc cttatcgtgt caccgacatt ataacacagg
      661 cgaaaaagat tgcaaacggg cgaaaaagca gagagacggc cgcctgagag ggccggagcg
      721 ggacgcagag aaaatccgcc ggtgcccgcg ggtgaaagac agaggcaaag cggcgcttgc
      781 ccggcccaaa accaccttgt gttttgcgcc tgcggcgagt ataattttgt tgtatcggtc
      841 ttgcggctgc cacggcttgg cggtgtgccg gggccggaaa aaagcggagg agttgacgaa
      901 atgtttggag atgtactgga cacgatcggc tttgtcaagg agcgcgaccc cgaggttggg
      961 gccgcgatgg agcgtgagat gggccgcctg cgcggaaaca tcgaactgat cgcctcggaa
     1021 aacgtggtct ccccggccgt gatggcggcg atgggcagca tcctgaccaa caaatacgcc
     1081 gagggctatc ccggccatcg gtattacggc ggctgccagt tcgtcgacga ggtcgaagag
     1141 ctggcccgga accgcgcctg cgagctgttc ggcgccgatc atgccaacgt ccagccccac
     1201 tccggcgcgc aggccaacct ggcggtctat tttgcgctgc tgcagcccgg cgacaccgtg
     1261 ctgggcatga acctgtccca cggcggccat ctcacccacg gcagcccggt caacatgtcc
     1321 ggcaagtatt tccgctttgt gccctacggt gtggatgccg acggcttttt ggattatgac
     1381 gcgctgcgcg agctggccct ggccgaaaag cccaagctga ttgtggccgg cgcctccgct
     1441 tatgcccgca ccatcgactt tgcccgcatc tccgagatcg ccaaagaggc gggctgcctg
     1501 ttcatggtgg atatggccca catcgccggc ctggtcgccg cgggcctgca cccgtcgccc
     1561 gtgccctacg cggacgtggt caccaccacc acccacaaga ccctgcgcgg gccccgcggc
     1621 ggcatgatcc tctgcaagga gcagtacgcc aaggccatcg acaaggccat cttcccgggt
     1681 acccagggcg gcccgctgat gcacgtcatc gccggcaagg cggtctgctt cggcgaggcg
     1741 ctcacccccg agttcaaaga gtatcagcgc cgcatcgtgg aaaacgccaa ggccctcagc
     1801 gaggccctgc tggcaaaggg cgtgcacctg gtctccgggg gcaccgacaa ccatctgatg
     1861 accgtggacc tgcgcggcac cggtgtcacc ggcaagcagc tggaggcgcg cttggacgag
     1921 gtgaacatca ccgccaacaa gaacaccgtg cccaacgacc ccgaaaagcc caccgtcacc
     1981 agcggcgtgc gcatcggcac cccggcggtc accacccgcg gcttcggcgt tgcagagatg
     2041 gaggagatcg cgggcttcat cgcgggctgt gtgttcgact ttgagggcaa aaaagaggag
     2101 atcaaggcgg gcgtcgcggc gctgacggcc cggttcccgc tgtacgagtg agttttcagg
     2161 gcgcggggct ccgcctctgc ggcggcccgc gccggaacgc ggctgttttt tgctttgccg
     2221 aaagcggggc aaggggggcg cgcaccggcg tgtgaggcgg caagaccgca ttccgcgcgg
     2281 gtacccggca cggtccttca tgagggccgc acgccgacgc gaggcgataa gatcacaaat
     2341 aaaaaacttt gggtgaaaca ggatggcata tatcaatgca aagctgcgca gtcaggtgct
     2401 gctgcgcacg gtggacgtgg cgctctatta tcccaacgac taccccgagg aggtcgtgcc
     2461 gggcattcgc ggcgtcttca cgctgctgca cggcttcaac ggctgtgggc aggactggct
     2521 gcagctctcg gccgcgacgc gttacgccgc ggacaacggg ctgatcctgg tggcgcccag
     2581 ctgtgagaat agcttttact gcgacatggt atacggcgac gcctggtata cctttctcac
     2641 cgacgagctg ccgctgctgc tggaaaaatt cttccgggtg cccaccgcgc gggagcagaa
     2701 ttttatcgca ggcctttcga tgggcggtta cggggcaatg atgctggcga tgacccaccc
     2761 ggagcgatac gcgggggcgg ccagcttttc gggcgcggtg gacctttcgc tgatgttaca
     2821 gcagggggcg cagcttcccg agctgcgcaa atcctttgtg cccatcttcg gcgagcagct
     2881 ggccctgccg caggacagag acctgcgggt gctggccggg cggctcagcg ggctgccgaa
     2941 agagcggcag cccaagctgc tgttcacctg cggcctcgag gacaacgagc cctattacat
     3001 ccatttacaa aaccgctcgc tctacgacag catccacacg ctgccctttg cggcctaccg
     3061 ctatatggaa tggccgggca accatgagtg gaagttctgg gaccgcagcc tcgtgtacgc
     3121 catcgactgg ttcctttcgc ccggctacgc cgaggcaaag cacggcgact ggcgcagcga
     3181 ggcgcagatc attccctgac aggagagaag tgttatgaac agccccctgg ccgaccgtct
     3241 tcggcccaaa acgctggacg aggtgttcgg ccagaagcag ctgctggcgc cgggcacggt
     3301 gttccggcgc aacatcgagg cgggtagggt gccgaacatg gt
//
LOCUS       DBMDYA010000279         3341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6445_length_3341_cov_1.462963, whole genome shotgun sequence.
ACCESSION   DBMDYA010000279 DBMDYA010000000
VERSION     DBMDYA010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_3341_cov_1.462963"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            242..1246
                     /locus_tag="ACZPFK_07650"
     CDS             242..1246
                     /locus_tag="ACZPFK_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1406222.1"
                     /translation="MKYRKRFLCAALAAMLLAGCSGRPADLGEGGGASPAPSSQASAP
                     AASSAGDVVFTDALGYQVELHSWQRVVSLYGSFAETWVLAGGALAGTTSDAVEERGLQ
                     LGEEVAVVGSIKEPNLEEILAADPDFVILSADTAAQVDLHAALVEAGVPHAYYRVDAF
                     DEYLAMLRQFCDMTGREDLYEENGLRVQQQVEAVLAAVEGRAHPTALLIRAFSTGAKA
                     KGDDNLTGVMLRDLGAENLVSRHESLLEDVSMEEVIAADPDFIFVITMGSSEEKAMEY
                     MRQNFEENPAWAGLTAVKNGRYVMLPKELFHYKPNARWGESYETLAKILYPDLAAQIG
                     "
     gene            1263..2234
                     /locus_tag="ACZPFK_07655"
     CDS             1263..2234
                     /locus_tag="ACZPFK_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1406223.1"
                     /translation="MAALGALLAGAVLLSLRCGSQDIPFAQLLAALRGGQQEGTVWRI
                     LVHVRVPRTLAALAAGCALAVAGALIQAVLNNVMASPNVIGVNAGAGFFALLAATLLP
                     AAGGLVPAASFAGALGTALFIYLLAVKAGLSRTTLILAGVAVSSILTAGVNALTLLYP
                     DQTVGAAGFMLGGFSGVTLAAVRGAAGYLLAGLLLAALLAMDLNVLQLGEESAAGLGL
                     HVGRVRFFAILAAALLAGGAVSFAGLLGFVGLLVPHMARRLVGGDNRWLLPASALLGG
                     GFVLLCDVLARVLFAPFELPVGIVMSLLGGPFFLSLLLRHKRSRVYD"
     gene            2227..2997
                     /locus_tag="ACZPFK_07660"
     CDS             2227..2997
                     /locus_tag="ACZPFK_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406224.1"
                     /translation="MIEMRAVSAGYEDTPVLQEVSFAVPRGSITALIGPNGCGKTTLL
                     RAAARQLPLRGGQILLSGRPVTAYGRKEFARTAAFLPQVRSVPAITVQALVTHGRFPY
                     LGLSRKLRPQDRAAVRCAMEETGVAAWAQRDLRELSGGERQRVYVAMALAQGTEVIFL
                     DEPTTYLDLHHQFELLELMRRLNARGKTILVVLHDLAHALRYSDQVVLMQAGRVVQAG
                     APGRVFESGAIDRVFGVRAHAAPDAYYFTPAPAAEAEG"
BASE COUNT          450 a         1061 c         1258 g          572 t
ORIGIN      
        1 tgggccgcgc cctgctgctg cggccgggca gcgacgtgac cgccgccgcc accgcccgcc
       61 ggtatccatc gcgctggacg ccgccgaggc cggcgcagtg ccttttcggg cctgcgcttc
      121 gggataaaaa agacaaactc cgcattggag cggcttgccg cgccggtgcg gctttttatg
      181 cgcgctgcgg cggcagggcc gcgccccctg aaaagagaaa aggcaaaaaa ggagaggtaa
      241 gatgaaatat agaaagaggt tcctctgcgc cgcgctggcg gcgatgctgt tggcgggatg
      301 ctccgggcgg cccgcggacc tgggcgaggg gggcggggcc tcccccgcac cgtcctccca
      361 ggcctctgcc cccgcggcct cctccgccgg ggacgtcgtt ttcaccgacg ccctgggcta
      421 tcaggtggag ctgcacagct ggcagagggt ggtctcgctc tacggcagct ttgcggagac
      481 ctgggtgctg gccggcggcg ctcttgccgg caccaccagc gacgcggtgg aggagcgggg
      541 tctgcagctg ggagaggagg tcgcggtggt gggcagcatc aaggagccca acctggagga
      601 gatcctggcg gcagacccgg actttgtcat cctctcggcc gacacggcgg cgcaggtgga
      661 cctgcatgcc gccctggtcg aggcgggggt tccacacgcc tactaccggg tggacgcctt
      721 tgacgagtac ctggccatgc tgcgccaatt ctgcgacatg accggccgcg aggacctgta
      781 tgaggagaac ggcctgcggg tgcagcagca ggtggaggcg gtgctggcgg ccgtggaggg
      841 ccgggcccac cccaccgccc tgctcatccg cgccttttcc accggcgcaa aggccaaggg
      901 ggacgacaac ctgaccgggg tgatgctcag ggacctgggg gcggaaaatc tggtctcccg
      961 gcacgagagc ctgttggagg acgtgagcat ggaagaggtg atcgccgccg acccggattt
     1021 tatctttgtc atcaccatgg gctccagcga ggagaaagca atggagtaca tgagacagaa
     1081 ttttgaggag aatcccgcct gggcggggct gaccgcggtg aagaacggcc gctatgtgat
     1141 gctgcccaag gagctgttcc actataagcc gaacgcgcgc tggggggaaa gttatgagac
     1201 gctggcaaag atcctctatc ccgatcttgc cgcccagatc ggttaagggc agggccgccc
     1261 tgttggcggc cctgggggcg ctgctggccg gggcggtgct gctgagcctg cgctgcggca
     1321 gccaggacat cccctttgcg cagctgctgg ccgccctgcg tggcggacag caggagggca
     1381 cggtatggcg catcctggtg catgtgcggg tgccccgcac gctggcggcg ctggcggcgg
     1441 gctgcgcgct ggcggtggcg ggggcgctga tccaggcggt gctcaacaac gtcatggcga
     1501 gccccaacgt gatcggggtc aacgcgggcg cgggcttttt cgccctgctc gccgccacgc
     1561 tgctgcccgc ggcagggggc ctggtgcccg ccgcgtcctt tgcgggggcg ctgggcacgg
     1621 cgctgttcat ctatctgctg gcggtgaaag ccgggctctc ccgtaccacc ctgatcctgg
     1681 cgggggtggc ggtcagcagc atcctcaccg ccggggtcaa cgccctgacg ctgctctacc
     1741 cggaccagac ggtgggggcc gccggcttta tgctgggcgg tttttccggg gtgacgctgg
     1801 cggcggtgcg cggcgcggcg ggctatctgc tggccgggct gctgctggcg gcgctgctgg
     1861 cgatggattt gaacgtgctg cagttgggcg aggagagcgc agcgggcctg ggcctgcacg
     1921 tgggcagggt gcgctttttc gccatcctgg cggcggcgct gctggccggc ggcgcggtca
     1981 gctttgcggg cctgctgggc tttgtgggcc tgctggtgcc ccacatggcg cgtcggctgg
     2041 tgggcggcga caaccgctgg ctgctgcccg cctcggcgct gctgggcggc ggctttgtgc
     2101 tgctgtgcga cgtgctggcg cgggtgctgt tcgccccatt cgaactgccg gtgggcatcg
     2161 tcatgtcgct gctgggcggc ccgtttttcc tcagcctgct gctgcgccac aaaaggagcc
     2221 gtgtttatga ttgagatgcg cgcggtgagc gccggatatg aagatacccc ggtgctgcag
     2281 gaggtctctt ttgcggtgcc gcggggcagc atcaccgccc tcatcggccc caacggctgc
     2341 ggcaagacca cgctgctgcg ggcggcggcg cgtcagctgc ccctgcgcgg gggacagatc
     2401 ttgttgtccg gccggccggt gacggcctat ggccgcaagg agtttgcgcg gacggcggcc
     2461 tttttgcccc aggtgcgcag cgtgcccgcc atcaccgtgc aggcgctggt cacccatggc
     2521 cgttttccct atctgggcct ctcgcgcaag ctgcgcccac aggaccgggc cgcggtgcgg
     2581 tgcgccatgg aggagaccgg ggtggcggcc tgggcgcagc gggacctgcg cgagctgtcg
     2641 ggcggggagc ggcagagggt ctatgtggcc atggcgctgg cgcagggcac cgaggtgatt
     2701 tttttggacg agcccaccac ctacctggac ctgcaccatc aatttgagct gctggagctg
     2761 atgcggcggc tcaacgcccg cggcaagacc attttggtgg tgctgcacga cctggcccac
     2821 gccctgcgct acagcgacca ggtggtgctg atgcaggcgg ggcgggtggt gcaggcgggc
     2881 gcgccgggcc gggtgtttga gagcggcgcg atcgaccgcg tgttcggggt gcgcgcccac
     2941 gccgcccccg acgcctatta tttcacccct gcccctgcgg cggaggcgga gggctgagcg
     3001 gaaaagtgtg cccttggact ccggggccca agggctgtaa aaacaagcgg gagggagggg
     3061 cgccggctct tgaaaccgga ggccctccct cccctttctc tgtggcgcac ctttcaggga
     3121 ccggggcgcg cagggatttt ccgttcgacg cgggggacag agctctgcgc ggcttccgac
     3181 ggagccgccg ccggggggcg cttctcccct gcgccgcagg ggcggaaaac ggcggcgcgc
     3241 cgccgctgct ttttgcccgc ggaggctgcc gggtcagacg gccgcacacc gacgcgggcg
     3301 gcctttttgc tctgcttttc ctcggtcccc ctgttttcgc a
//
LOCUS       DBMDYA010000280         3339 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6454_length_3339_cov_1.384497, whole genome shotgun sequence.
ACCESSION   DBMDYA010000280 DBMDYA010000000
VERSION     DBMDYA010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6454_length_3339_cov_1.384497"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(19..588)
                     /locus_tag="ACZPFK_07665"
     CDS             complement(19..588)
                     /locus_tag="ACZPFK_07665"
                     /inference="COORDINATES: protein motif:HMM:NF025635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1406225.1"
                     /translation="MSNQAFTGGVRPGGLTTSTEIRILLCYLLKSIGIPLTRQEIENA
                     LLSEELVNYFELSDALDKLCALGHLTLEGDAYRVTASGSDLADTLEGEVPRSVRESAV
                     NAVLTAQQYKRKQAQHKAQVSRAADGWRVRCTIEDLGEPVFSFELYLPDELTANAVKE
                     KFIADGDDVFRCMLGLLTDNRQLLSGLLG"
     gene            complement(733..1662)
                     /locus_tag="ACZPFK_07670"
     CDS             complement(733..1662)
                     /locus_tag="ACZPFK_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR1406226.1"
                     /translation="MIYAAEDRLPCCTDFTLRELMEAAEKHTVLQSTALLYDTDKSLH
                     FCLGGYPALMPHEECAEGIRTGQVRDIAVITRVGRAACFVITEAVGGPRPRFTLSRAE
                     AQRRCRQEYLDRLQPGDIIPCTATHIEPFGVFCDVGCGISALLPIDCLSVSRIASPAD
                     RVRPGQPLTCVVRQRDERGRLVLSMKELLGSWQDNADLFKVGMSVMGIVRSVEDYGVF
                     IELTPNLAGLAEYTDTLSPGDAVSVYIKNILPQKMKIKLVVLSHLPAPAAPAPLHLFI
                     TEGHLDEWVYSCPGARRLVRTVFSEEMPARGGS"
     gene            1936..2124
                     /locus_tag="ACZPFK_07675"
     CDS             1936..2124
                     /locus_tag="ACZPFK_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1406227.1"
                     /translation="MDVQVGDILQMKKPHPCGGGEFAVLRVGMDFRVRCVKCGREVMV
                     PRAKIERHIKKVIREAER"
     gene            2180..3256
                     /gene="prfA"
                     /locus_tag="ACZPFK_07680"
     CDS             2180..3256
                     /gene="prfA"
                     /locus_tag="ACZPFK_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1406228.1"
                     /translation="MLDKLNEVKRHYEEIGNQLTDPAVIRDNGLYRSLMKEHKNLTPL
                     IEKYDEYCRAKQAFEEARALLDEGGLEPDFKEMVQTEYAESRAQCEQAVQELKVLMLP
                     RDENDDKNIIMEIRGGTGGEEAALFAHSLYRMYTMYAEARRWNIEVNSVSETELGGIK
                     EISFTVNGEGAYSRLKFEGGTHRVQRVPETETQGRVHTSAATVAVMPEAEEVELELDP
                     KDLKIDTFRSSGAGGQHINKTSSAIRVTHLPTGMVVECQDERSQYKNKDKALRVLRSR
                     LLQQKQAAQHAEISAERKSQVGTGDRSGRIRTYNYPQGRVTDHRIGLTLYKLDAVLDG
                     DLDEVIDALVTADQAEKLKSSAEG"
BASE COUNT          678 a         1004 c         1131 g          526 t
ORIGIN      
        1 tggcggacag ccggggaatc agcccagcag accggacagc agctgccggt tgtcggtcag
       61 caggcccagc atgcagcgga acacatcgtc gccgtccgcg atgaactttt cttttaccgc
      121 gttggcggtc agctcgtcgg gcagatagag ctcaaaggaa aagaccggct cgcccaggtc
      181 ctcgatggtg cagcgcaccc gccagccgtc tgccgccctg gagacctgcg ccttgtgctg
      241 cgcctgtttg cgcttgtact gctgggcggt gagcaccgcg ttcaccgcgc tctcgcgcac
      301 cgagcggggc acctcgccct ccagggtgtc cgccaggtca gagccgctgg cagtcacgcg
      361 gtaagcgtcc ccctccagcg tcaggtggcc cagtgcgcag agcttgtcga gcgcatccga
      421 gagctcaaaa tagttgacca gctcctcgct cagcagcgcg ttttcgatct cctgccgggt
      481 gagagggatg ccgatgctct tgagcagata gcacagcagg atgcggatct cggtcgaggt
      541 cgtcagcccg cccggccgca caccgccggt aaatgcctgg ttggacatcg gttggcctcc
      601 ttcgtttgac aaagccggtt gtagggccgc ccacaggcct gcccccaggg gcggggccgc
      661 gggccgcacc aattacaact cagagaaaaa gtgatgagaa tagaataagt atagcacagt
      721 ttttcagctt actcaactgc caccgcgggc cggcatttct tctgaaaaga cggtgcgcac
      781 cagccgccgc gccccagggc aggaatagac ccattcgtcc aggtgtccct cggtgatgaa
      841 aaggtgcagc ggggccgggg ccgcgggggc aggcaggtgg ctgagcacca ccagcttgat
      901 cttcattttt tggggcagga tatttttgat gtacacgctg accgcatccc cgggagaaag
      961 cgtgtcggtg tactcggcaa gaccggccag gttgggggtc agctcgatga agacgccgta
     1021 gtcctccacg ctgcgcacaa tgcccatcac gctcatgccg accttgaaca ggtccgcgtt
     1081 gtcctgccag ctgcccagca gctctttcat gctcagcacc agccgtccgc gctcgtcccg
     1141 ctggcgcacc acgcaggtga gcggttggcc gggacgcacc cggtcggcgg gcgaggcgat
     1201 gcgggacacc gagaggcagt cgatgggcag cagcgcgctg atgccgcagc ccacgtcgca
     1261 aaagacgccg aaaggttcga tgtgggtggc ggtgcagggg ataatatccc ccggctgcag
     1321 ccggtcaaga tactcctgcc ggcagcggcg ctgggcctcg gcgcgggaga gggtaaagcg
     1381 gggccggggc ccgcctacgg cctcggtgat gacaaaacag gcggcgcggc ccacccgcgt
     1441 gatcaccgcg atgtcccgca cctgcccggt gcgaatcccc tcggcgcact cctcgtgggg
     1501 catcagggcg gggtagccgc ccaggcagaa gtgcaggctt ttatcggtgt cgtacagcag
     1561 ggcggtgctc tgcaggaccg tgtgcttttc cgcagcttcc atcagttcgc gcagggtaaa
     1621 gtcggtgcag cagggcagcc ggtcctccgc agcgtagatc attttggttt cctccttaaa
     1681 agcgcgcggt ttgcagggtg aaaagaggcc tcacgcccgg gcgcggcgcc ggtgataagg
     1741 ggcgggaaga cggatgccct cacacgaccc cccggcccgt gtgagggcat cctcgtccca
     1801 gccctgtcat ctaaactata tgccgcgtcc acagtgaata tttgcacaag cggggtgaat
     1861 gtactataat atataagact gccgggcggc cgaaatgtgc cgccggcaca gaaaaaaacg
     1921 gccgaggtgg agcgcgtgga cgtgcaggta ggggatattc tgcagatgaa aaagccgcat
     1981 ccctgcggcg gcggcgagtt cgcggtgctg cgggtgggga tggatttccg cgtccgctgc
     2041 gtcaagtgcg ggcgggaggt catggtgccc cgcgcaaaga tcgagcgcca tatcaaaaag
     2101 gtcatccggg aggcggagcg atagcgcggc cccttttgcg gcgtttttgg aaaacagagc
     2161 aaaaaaggag cgcatggaga tgctggacaa actgaatgag gtcaaacgcc attacgagga
     2221 gatcggcaat cagctcaccg accccgccgt catccgcgac aacggccttt accgcagcct
     2281 gatgaaagag cataaaaacc tgacccctct catcgaaaag tacgacgaat actgcagggc
     2341 gaagcaggcg tttgaggagg cgcgggccct gctggacgag ggcgggctgg agccggattt
     2401 caaagagatg gtgcagacgg agtacgcgga aagccgcgcc cagtgcgagc aggcggtgca
     2461 ggagctgaaa gtgctgatgc tgccgcggga tgaaaacgac gacaagaaca tcatcatgga
     2521 gatccggggc ggcaccggcg gcgaggaggc ggcgctgttc gcccacagcc tttaccgcat
     2581 gtataccatg tatgccgagg cccgccggtg gaacatcgag gtcaacagcg tcagcgagac
     2641 ggagctgggc ggcatcaagg aaatcagctt tacagtgaac ggcgagggcg cctacagccg
     2701 tctcaagttc gagggcggca cccaccgggt gcagcgggta ccggagaccg agacccaggg
     2761 ccgggtacac acctcggccg ccaccgtggc ggtgatgccg gaggccgaag aggtggagtt
     2821 ggagctggac cccaaggact tgaagatcga cacgttccgc tcctccggcg cgggcggcca
     2881 gcacatcaac aagacctcct ccgccatccg ggtgacccat ctgcccaccg gcatggtggt
     2941 ggagtgtcag gacgagcgca gccagtacaa gaacaaggac aaggccctgc gggtgctgcg
     3001 cagccgcctg ctgcagcaaa agcaggcggc ccagcacgcg gagatctccg ccgagcgcaa
     3061 gagccaggtg ggcacgggcg accgcagcgg ccgcatccgc acctacaact acccgcaggg
     3121 gcgggtgacc gaccatcgca tcggcctgac gctgtacaag ctggacgcag tgctggacgg
     3181 cgatttggat gaggtcatcg atgcgctggt gaccgcggat caggcggaaa aactcaagtc
     3241 ctctgcggag ggataggaga cgaaagctat ggagacaaaa ctgcttgccg ccgggccgga
     3301 ggcagtggcc gaggcggcgc ggctgctgcg cgcggggga
//
LOCUS       DBMDYA010000281         3337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6461_length_3337_cov_1.209209, whole genome shotgun sequence.
ACCESSION   DBMDYA010000281 DBMDYA010000000
VERSION     DBMDYA010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_3337_cov_1.209209"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            545..1729
                     /locus_tag="ACZPFK_07685"
     CDS             545..1729
                     /locus_tag="ACZPFK_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1406229.1"
                     /translation="MDIKALAEKYESYIIEQRRWFHAHPELSWEEYKTTDAIAAQLEG
                     MGLEVQRFTDGLAGCTAVIRGGKAKPGCKTVALRADIDALPVEEKTGLPFASENAGCM
                     HACGHDNHIAMLLGAAKILTEMQDELEGNVKLFFQPAEESCHGAEPCIAQGVLEGVDA
                     IYGQHIWDGLEAPYLNVQPGVRMASCDNFSITVEGSSSHGSAPNLGVDAIVAASAIIM
                     NLQTIVSRNNPPLNGLVVSVGEIHGGQRWNVVAGKVVMEGTCRTHSREMRGQIEPLIR
                     RVAESTAAAYNARATLKYEAFPGPVINDQDDLNQIAHDAAVKLYGEEGIKELPKMMGS
                     EDFAYFMEKVPGVFGFLGSHDAQHHASNHNDHYDVPEEVLKRGAAMHAQFAADYLAAK
                     AE"
     gene            1768..2940
                     /locus_tag="ACZPFK_07690"
     CDS             1768..2940
                     /locus_tag="ACZPFK_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1406230.1"
                     /translation="MNVRELAEKYESYIIERRRYYHTCPELSGQEVETAKSLKADLEA
                     MGVEVEMCKNCNGLVGTIRGGKPGKTVALRADIDALSVVEETGLPFASKNEGAMHACG
                     HDNHMAMLLGGAKILTEIKDQMPGTVKLLFQPAEEVATGAKSMIAEGVLEGVDAIYGA
                     HIWGDFDAPLFDASAGNRMACCDGFTIEVEGVSTHGSSPHLGTDGIVAAAAIIQELQS
                     YVSRNNNPLNPCVVTVGTINGGQRFNIIANKVVLEGTVRTFSRETLATIQGDMQRIVS
                     NTAAAFGATANVTKYSHMTPPLINDNEELNKIAQDAVVKLYGEDALGHLPTMMGSEDY
                     AFFMEKVPGIYCFLGSRDAEHHYINHHEKYDVPEDVLKRGAAMYAQFAFDFLEANA"
     gene            3070..>3337
                     /locus_tag="ACZPFK_07695"
     CDS             3070..>3337
                     /locus_tag="ACZPFK_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406231.1"
                     /translation="MAICKDCQPFLHPAFFKTAGLKLPGHPNEEKLGCRTRKIHADRL
                     RRCLFACLRRRFAGRNRKPLWGHGANVTALCAQTSLAGQCFPAET"
BASE COUNT          705 a         1067 c         1047 g          518 t
ORIGIN      
        1 ctttcaagcc tgatgttgag gcgactcacc agcccggaga ttgggaagcg ccccccgccg
       61 caccgcaccc gggcgaccgg agagagggaa aaattcccgg gcacacaaaa acaggaggcg
      121 ggaagcggct ttcaagcctg atgttgaggc gactcaccag cccggagatt gggaagcgcc
      181 ccccgccgcg ccgcactcgg gcgcccgcag agagggaaga gtcccccggc tcacaaaggc
      241 aggaggcggg aagcagggcg gcggaccttt tgtcagcgcg ggtctgggcg ctttcggcgt
      301 cggtttgaaa cagttttctt cgccggtcaa gcgtttttca agcgggcaaa aacgcacttt
      361 ttcgccgcca gtttcaaaat atagccggaa agttgcgcca aatcccacaa aacttctgtt
      421 caagccctag ggacctgtgt tacaatgaaa ccatcaaagt acctgccgcg cgcccggcga
      481 cccgccgcag cagtgccacc ggccggctgc ggcgcaggcg cggcacaaaa ggaggagaac
      541 gacaatggat atcaaagctt tggcggaaaa gtatgagagc tatatcatcg aacagcgccg
      601 ctggttccac gctcatcccg agctgtcctg ggaggagtat aagaccaccg acgccatcgc
      661 cgcgcagctg gagggcatgg gcctggaggt ccagcgcttc accgacggcc tggcgggctg
      721 caccgcggtg atccgcggcg gcaaggcaaa gcccggctgc aagaccgtgg ccctgcgcgc
      781 cgacatcgac gcgctgcccg tggaggaaaa gaccggcctg ccctttgcca gcgagaacgc
      841 cggctgcatg cacgcctgcg gccacgacaa ccacatcgcc atgctgctgg gcgccgcgaa
      901 gatcctgacc gagatgcagg acgagctgga gggcaacgtg aagctctttt tccagcccgc
      961 ggaggagagc tgccacggcg ccgagccctg catcgcacag ggcgtgctgg agggcgtgga
     1021 cgccatctac ggccagcaca tctgggacgg cctggaggcc ccctacctga acgtgcagcc
     1081 cggcgtgcgc atggccagct gcgacaattt ctcgatcacc gtcgagggct cctcttccca
     1141 cggcagcgcc ccgaacctgg gcgtggacgc catcgtggcg gcctccgcca tcatcatgaa
     1201 cctgcagacc atcgtctcgc gcaacaaccc ccccctcaac ggcctggtgg tcagcgtggg
     1261 cgagatccac ggcggccagc gctggaacgt ggtggcgggc aaggtggtca tggagggtac
     1321 ctgccgcacc cattcccgcg agatgcgcgg ccagatcgag ccgctcatcc gccgcgtggc
     1381 cgagtccacc gccgccgcct acaacgccag ggcgaccctg aagtacgagg cgttccccgg
     1441 cccggtcatc aacgaccagg acgacctgaa ccagatcgcc cacgacgccg cggtcaagct
     1501 gtacggcgag gagggcatca aagagctgcc caagatgatg ggcagcgagg actttgccta
     1561 tttcatggaa aaagtgcccg gcgtttttgg gttcctgggc agccatgacg cccagcacca
     1621 cgccagcaac cacaacgacc actacgacgt gccggaggaa gtgctcaagc gcggggcagc
     1681 catgcacgcc cagtttgcag ccgattatct ggcggccaag gcggaatgac cgtcgccgaa
     1741 aaaatggaaa acagaggagt gtatcccatg aacgttcgtg aactcgctga aaaatacgaa
     1801 tcctatatca ttgagcggcg gcgctattac cacacctgcc cggagctgtc cgggcaagag
     1861 gtggagaccg ccaaaagcct gaaagccgac ctggaggcca tgggcgtcga ggtcgagatg
     1921 tgcaaaaact gcaacggcct ggtgggcacc atcaggggcg gcaagcccgg caagaccgtg
     1981 gccctgcggg ccgatatcga cgcgctgtcg gtggtggagg aaaccggcct gccctttgcc
     2041 agcaaaaacg agggcgccat gcacgcctgt ggccatgaca accacatggc catgctgctg
     2101 ggtggcgcaa agatcctcac cgagatcaag gaccagatgc ccggcaccgt gaagctcttg
     2161 ttccagcccg cggaggaggt cgccaccggg gccaagtcga tgatcgccga gggcgtgctg
     2221 gagggcgtgg acgccattta cggcgcgcac atctggggcg attttgacgc gccgctgttc
     2281 gacgcctccg ccggcaaccg catggcctgc tgcgacggct tcacgatcga ggtggagggc
     2341 gtttccaccc acggcagcag cccgcatctc ggcaccgacg gcatcgtggc cgcggcggcg
     2401 atcatccagg agctgcagtc ctatgtctcc cgcaacaaca acccgctcaa cccctgcgtg
     2461 gtcaccgtgg gcaccatcaa cggcggccag cgcttcaaca tcatcgccaa caaggtggtg
     2521 ctggagggca cggtgcgcac gttctcacgc gagaccctgg cgaccatcca gggcgatatg
     2581 cagcgcatcg tcagcaacac tgcggccgct tttggggcca cggccaacgt gaccaagtac
     2641 agccacatga ccccgccgct gatcaacgac aacgaggaac tgaacaagat cgcgcaggac
     2701 gcggtggtca agctgtacgg cgaggatgcc ctgggccacc tgcccaccat gatgggcagc
     2761 gaggactatg cgttcttcat ggagaaagtc cccggcatct actgcttcct gggcagccgc
     2821 gacgcggagc accactacat caaccaccac gagaaatacg acgtccccga ggacgtgctg
     2881 aaacgcggcg cggcgatgta cgcccagttc gctttcgatt ttctcgaggc gaacgcctga
     2941 gcagccgcac ccgcgcgagt cggtttgccg ggccggttta aaaaggtaag gcgcgcgccc
     3001 cttggacgcg ttgaggtgag aaaacattgg ttttgaaccg acggctttga tgctgcgtat
     3061 tgtgaaaggt tggcaatctg caaagattgc caaccttttt tgcatcccgc tttcttcaaa
     3121 accgccggtt taaaactgcc cggccatccc aacgaggaaa agctgggatg cagaaccaga
     3181 aaaatccatg cggatcgcct gcggcgctgt ctgtttgcct gcctgcgcag gcggtttgca
     3241 gggcgaaatc gcaaacccct ctgggggcac ggggcgaatg tgacggcgct ctgcgcgcaa
     3301 acctccctcg cggggcagtg ctttcctgct gaaacgc
//
LOCUS       DBMDYA010000282         3335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6462_length_3335_cov_1.827149, whole genome shotgun sequence.
ACCESSION   DBMDYA010000282 DBMDYA010000000
VERSION     DBMDYA010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_3335_cov_1.827149"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            611..1246
                     /locus_tag="ACZPFK_07700"
     CDS             611..1246
                     /locus_tag="ACZPFK_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1406232.1"
                     /translation="MRLKAKFGQMCKDTLLICAGLLVMALGSALVYQAALGTGSIGTM
                     VDGVHQALGISRGLADITVSLVLLAVVCLTARDLINIGTVISLFFTGFSIDFWKWVLT
                     FLPAPSAAGVYAMYAAGLMLGSMGTGFYIAVNAGISAYDAFIMTVYRLTRWQYRYAKI
                     LADFLLMLAGILLGGTIGVGTVISTVAGGFLTQFFIQRSTRLLGRRAVLTN"
     gene            1336..2487
                     /locus_tag="ACZPFK_07705"
     CDS             1336..2487
                     /locus_tag="ACZPFK_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR1406233.1"
                     /translation="MKKFLATLMSITLAAALLLTGCGGGGGSSAAGSGSAPQAGGTAG
                     KDAKNIVYVTSSSLGDDPLVDLVWDSVKSAGEDFGMNTKCVELNNDTSLYASSVMDLC
                     ESGEWDLIITGFFQMIDPVCEAVAEYPDQHFLVFDSSLDYSSGEFENCVSVEGLQNEG
                     SFLAGVLAGCLTTSGQDGFNDDKVIGCVLANPGMLDDFLAGYIDGAKWVDPAIEVLYS
                     YNGSFSDTATASEHALAQFNRGADIVYSVNGAAGLGVADAALQAGHYMIGVDTDLAAE
                     VSGNNAAMAERIVTSVCKDFGTILYDQLEKYAAGTLEFGKHTRYGLADHGMFLIDNEY
                     FDAVATDEIKAKLKEAEEKIASGEIKVSTTIGVSDEEYAEILSRASAAE"
     gene            2618..>3335
                     /locus_tag="ACZPFK_07710"
     CDS             2618..>3335
                     /locus_tag="ACZPFK_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1406234.1"
                     /translation="MGSTTQTAADVLSMQHITKIYGNGFMANKDICFSVRKGEIHGLV
                     GENGAGKSTLMKVLFGQEVPEEGQIFIGGEEVKISNPLVALDYGIGMVHQHFMLVPSL
                     TVAENMVLGVEPRKGKLFFDYKKAARMTREVAEKYNLPINPDKRVMDLSVGYKQRVEI
                     LKILLRGAKILLLDEPTAVLTPQETAELFVQLKNLKKEGFTIVFISHKLNEIKEICDR
                     ITVLRDGQVVGTAEIGEVSEQ"
BASE COUNT          711 a          895 c         1050 g          679 t
ORIGIN      
        1 ctcacaggag agaggcgcag cctgaaaatg tcaaaaattc ttattttatg aatttttatt
       61 ctttttgttt ttgcactttt gtgctttggg acggctgttt gcagccgccg tttcccgccg
      121 ggcaatcagg cgcggcgagc aggaatcgtt caccgcgatg gtgatgtgcg gggataaaat
      181 gataatttat tgctgtcatg gggaaagaaa cctgattttc agcctctgcc ggcctcgaaa
      241 aatcctctcg gctttttcga gacggcgaag agaaattcca ggattatagt gccaaaaaga
      301 ggaaaaaaca ataaaaaaaa gtggaatgag agcaaaaaat aaatcaatct acccaaaaaa
      361 tgggggttga catccgctgg gattcttgct atgatgacgt tgcagatgtg aattcacact
      421 ctcgcaggag ggaatgttgc aaagcaatat atttatatta ttttatataa tatataaggc
      481 tttccccacg cgaaacaaaa attgaataca gattttctga cgaggtaata gctgactgaa
      541 tcaaaaagtt gtagcatact gacttggcct ttgggttgag aagtatgcta ctttttttta
      601 gggagggagt atgcggctca aagcaaagtt cggccaaatg tgcaaagata cgcttttgat
      661 ctgcgcgggg ctccttgtga tggccctggg ctccgcgctg gtttaccagg cggcgctggg
      721 caccggttcc atcggcacca tggtggacgg ggtgcatcag gccctgggca tctccagggg
      781 tcttgcggat atcacggtga gcctggtttt gctggcggtc gtctgcctga cggccaggga
      841 tctcatcaac atcggcaccg tgatcagctt gttcttcacc ggcttttcca tcgatttctg
      901 gaagtgggtg ctcacctttc tgccggcgcc ctccgcggcg ggggtctacg ctatgtacgc
      961 ggcggggctg atgctggggt cgatggggac gggcttttac atcgcggtca acgcgggtat
     1021 cagcgcctac gacgctttta tcatgacggt ctaccggctc acccgctggc agtaccgcta
     1081 cgccaagatc ctggcggatt ttttgctgat gctggcgggc atcctgctgg gcggcaccat
     1141 cggggtgggc acggtgatca gcacggtggc gggcggcttt ctgacccagt tttttatcca
     1201 gcgcagcacc cggctgctgg gccgccgcgc ggtgctgacc aactgacctg tttctgtttt
     1261 tgtgagaaga ccccggatgc ggttctgggc cacggcgggg aaaagtttca gaaaagattt
     1321 taggaggaaa gaaccatgaa aaagttttta gccactctca tgagcatcac tctggcggcc
     1381 gctttgctgc tgaccggctg cggcggtggc ggcggttcgt ccgccgcggg gtccggctct
     1441 gcgccccagg cgggcggcac ggcgggcaag gacgccaaaa atatcgtcta cgtcaccagc
     1501 agctcgctgg gcgacgaccc gctggtggat ctggtctggg attcggtgaa gagcgccggc
     1561 gaggatttcg gcatgaacac caagtgcgtc gagctcaaca acgacacctc actgtatgcc
     1621 tccagcgtca tggacctgtg cgagtccggc gagtgggacc tgatcatcac cggcttcttc
     1681 cagatgatcg acccggtctg cgaggcggtg gccgagtacc cggatcagca ctttttggtc
     1741 tttgacagct cgctggatta cagcagcggc gagtttgaaa actgcgtgtc ggtggagggc
     1801 ctgcagaacg agggctcttt tctggccggc gtgctggcgg gctgcctgac caccagcggc
     1861 caggacggct tcaacgacga caaggtcatc ggctgcgtgc tggccaaccc cggcatgctg
     1921 gacgatttcc tggccgggta catcgacggc gcgaagtggg tggaccccgc cattgaggtc
     1981 ctctactcct acaacggcag cttcagcgat accgccaccg cctccgagca cgccctggcc
     2041 cagttcaacc gcggcgcgga catcgtctac tcggtcaacg gggccgccgg cctgggcgtg
     2101 gcggacgcgg ccctgcaggc gggccattac atgatcggcg tggacaccga cctggcggcc
     2161 gaggtctcgg gcaacaacgc ggccatggcg gagcggatcg tcacctccgt gtgcaaggat
     2221 ttcggcacca tcctttacga ccagctggaa aaatacgcgg ccggtacgct ggagttcggc
     2281 aagcacaccc ggtacggcct ggccgaccac ggcatgttcc tgatcgacaa cgagtacttt
     2341 gacgcggtcg ccaccgacga gatcaaagcc aagctgaaag aggccgagga gaagatcgct
     2401 tccggcgaga tcaaggtgag caccaccatc ggggtcagcg acgaggagta cgccgagatc
     2461 ctcagccgcg ccagcgccgc cgagtaaggc gtcgggacgc gcggcggcgc aggcgccccc
     2521 agaggcgttt gcgtcacccg accggtaaag ggagcccggc ccgagcgggc cgggcttttt
     2581 attccggcac aatgaaaaca ggcaaggagg aatgaatgtg ggcagcacga cgcagacggc
     2641 ggccgacgtc ctttcgatgc agcatatcac caagatctac ggcaacggct ttatggcaaa
     2701 caaggacatc tgcttcagcg tgcgcaaggg cgagatccac gggctggtgg gcgaaaacgg
     2761 cgccggcaag tccaccctga tgaaagtgct ctttgggcag gaggtgccgg aggaggggca
     2821 gatcttcatc ggcggggaag aggtcaaaat cagcaaccct ctggtggcgc tggattacgg
     2881 catcggcatg gtgcatcagc attttatgct cgtgccctcg ctgacggtgg ccgaaaatat
     2941 ggtgctgggt gtggagccgc gcaagggcaa gctgtttttt gactacaaaa aggcggcccg
     3001 catgacccgg gaggtggcgg aaaagtacaa tctgcccatc aacccggaca agcgggtgat
     3061 ggacctgtcg gtgggctata agcagcgggt ggaaatcctc aagatcctgc tgcgcggcgc
     3121 aaagattttg ctgctggacg agcccacggc ggtgctcacc ccgcaggaga cggccgagct
     3181 ctttgtacag ctgaaaaatt tgaaaaaaga gggcttcacc atcgtcttca tctcccataa
     3241 gctgaacgag atcaaagaga tctgcgaccg catcaccgtg ctgcgggacg gccaggtggt
     3301 gggcacggcc gagatcggcg aggtcagcga gcagg
//
LOCUS       DBMDYA010000283         3324 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6484_length_3324_cov_1.317096, whole genome shotgun sequence.
ACCESSION   DBMDYA010000283 DBMDYA010000000
VERSION     DBMDYA010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6484_length_3324_cov_1.317096"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="ACZPFK_07715"
     CDS             <1..936
                     /locus_tag="ACZPFK_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1406235.1"
                     /translation="TYVMCVEELSKVCATTGVIVSAHTSLGTDPIKKFGTPLQQEKYL
                     KKLASGEYLGAFGLTEPGAGTDASGQQTKAVLDGDHYVLNGSKIFITNGGQADTYIIF
                     AMTDKSKGTKGISAFIVEKDFPGFSIGKKEDKLGIRASSTTELIFENCIVPKENLLGA
                     EGKGFGIAMQTLDGGRIGIAAQALGIAEGALDEAKKYVNERKQFGRPISKFQNTQFVF
                     ADLATKVEAAKLLVYKAAMAKDTQKRFSVEAAMAKLFAAETAMEVTTKVVQMFGGYGY
                     TRDYPVERMMRDAKITEIYEGTSEVQRMVIAGSLL"
     gene            961..1743
                     /locus_tag="ACZPFK_07720"
     CDS             961..1743
                     /locus_tag="ACZPFK_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HIR1406236.1"
                     /translation="MNIVVCIKQVPDTNEVKIDPVTGTLIRDGIPSIMNPDDKAGLEA
                     ALRIKEQNGAHVAVVSMGPPQADAVLREALAMGADEGYLITDRAFGGADTWATSSTIA
                     SAIEKLPYDLIITGRQAIDGDTAQVGPQIAEHLGLANISYAEDIKVEGDSVVVKRQFE
                     DRYHVLKAKMPCLITALSELNEPRYMTPGGIFDAYREGDVKTFTRPDLAVSDDNIGLK
                     GSPTRVFKSFTKALKAAGTKVQLDPQESADYIVEKLKEKFII"
     gene            1838..2851
                     /locus_tag="ACZPFK_07725"
     CDS             1838..2851
                     /locus_tag="ACZPFK_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR1406237.1"
                     /translation="MNLQDYKGVFVFVQQVDGKITNVSYELIGKAKELAADLGTEVTA
                     VLLGYNVDGLCKPLAEYGADRIIKIDDKLLETYMTEPYAHCMAETINKYKPEAVLVGA
                     TAIGRDVGPRISARVHTGLTADCTGLAVDPESKNLQMTRPAFGGNIMATIVCPEHRPQ
                     MATVRPGVMKKLPREEGRTCPVEEFVPADLAQHINVEILEVVKTAAAKMDIQDAKILV
                     SGGRGMCSAENFHLLQELADVLGGTVSCSRAAVDAGWAEKDMQVGQTGKTVRPNLYIA
                     CGISGAIQHLAGMEDSDVIVAINKDETAPIFEVADYGIVGDALQIVPLLTEALKREIV
                     AKG"
BASE COUNT          749 a         1042 c          988 g          545 t
ORIGIN      
        1 acctacgtca tgtgcgtgga ggagctgtcc aaggtctgcg ccaccaccgg cgtcatcgtg
       61 tccgcccaca cctcgctggg caccgacccc atcaaaaagt tcggcacccc gctgcagcag
      121 gagaaatacc tgaagaagct ggcctccggc gagtatctgg gcgctttcgg tctcaccgag
      181 ccgggcgccg gcaccgacgc ctctggccag cagaccaagg cggtgctgga cggcgaccac
      241 tatgtgctca acggcagcaa gatctttatc accaacggcg gtcaggccga cacctatatc
      301 atcttcgcga tgaccgacaa gtccaagggt accaagggca tctcggcttt catcgtagaa
      361 aaggacttcc ccggcttctc aatcggcaaa aaggaggaca aactgggcat ccgcgcctcc
      421 tccaccaccg agctgatctt tgaaaactgc atcgtgccca aggagaacct gctgggcgcc
      481 gagggcaagg gcttcggcat cgccatgcag accctggacg gcggccgcat cggcatcgcc
      541 gcgcaggcgc tgggcatcgc cgagggcgct ctggacgagg cgaaaaaata cgtcaacgag
      601 cgcaagcagt tcggccggcc catctccaag ttccagaaca cccagttcgt ctttgcggac
      661 ctggccacca aggtggaggc cgcgaaactg ctggtctaca aggctgcgat ggcgaaagac
      721 acccagaagc gtttcagcgt cgaggccgcg atggcgaagc tgtttgccgc cgagaccgcg
      781 atggaggtca ccaccaaggt ggtgcagatg ttcggcggtt acggctacac ccgcgactac
      841 ccggtggagc gcatgatgcg cgacgccaag atcaccgaga tctacgaggg caccagcgaa
      901 gtgcagcgca tggtcattgc cggcagtctg ctgtaacgag agggaaggag agtctatacc
      961 atgaacatcg tagtttgcat caagcaagtt cccgatacca acgaggtcaa gatcgacccc
     1021 gtgaccggca ccctgatccg cgacggcatc cccagcatta tgaacccgga cgacaaggcc
     1081 ggcctggagg ccgcgctgcg catcaaagag cagaacggcg cccacgttgc cgtcgtctcg
     1141 atgggcccgc cccaggcgga cgccgtgctg cgcgaggccc tggccatggg cgcggacgag
     1201 ggctacctca tcaccgaccg cgcctttggc ggcgcggata cctgggccac ctccagcacc
     1261 atcgcctcgg ccatcgaaaa gctgccctat gacctgatca tcaccggccg gcaggccatc
     1321 gacggcgaca ccgcacaggt tggcccgcag atcgccgagc acctgggcct ggcgaacatc
     1381 agctatgccg aggacatcaa ggtcgagggg gattctgtgg tcgtcaagcg ccagtttgaa
     1441 gaccgctatc atgtcctcaa ggcgaaaatg ccttgcctga tcaccgctct gtccgagctc
     1501 aacgagccgc gctacatgac cccgggcggc atcttcgacg cctaccgcga gggggacgtc
     1561 aagacgttca cccgccccga tctggccgtc tccgacgaca atatcggcct caagggcagc
     1621 cccacccgcg tcttcaagtc ctttaccaag gcgctcaagg ccgccggcac caaggtgcag
     1681 ctggacccgc aggagtccgc tgattacatc gtcgagaagc tcaaagaaaa attcattatc
     1741 tgacagaacg cggtgacgcg ccgcgcgccc ggcgggccct ggcccccgtt gcgcccggcg
     1801 tgaccggcac aattccgaga aaggaatgat cattcaaatg aatcttcagg attataaagg
     1861 tgtattcgtc tttgtgcagc aggtcgacgg caagatcacg aacgtctcct acgagctgat
     1921 cggcaaggcg aaagagctgg ccgcggacct cggcaccgag gtcaccgccg tgctgctggg
     1981 ctacaacgtg gacggcctgt gcaagcccct ggccgagtat ggcgccgacc gcatcatcaa
     2041 gatcgacgac aagctgctgg agacctacat gaccgagccc tatgcgcact gcatggccga
     2101 gaccatcaac aaatacaagc ccgaggccgt tctggtcggc gcgaccgcca tcggccgcga
     2161 cgtgggcccc cgcatctccg cccgtgtgca caccggcctg accgccgact gcaccggcct
     2221 tgccgtggac ccggagagca agaacctgca gatgacccgc ccggcctttg gcggcaacat
     2281 catggcgacc atcgtctgcc ccgagcaccg cccccagatg gccaccgtgc gccccggcgt
     2341 gatgaaaaag ctgccccgtg aggaggggcg cacctgcccc gtagaggagt tcgtgcccgc
     2401 cgacctggcg cagcatatca acgtggagat cctcgaggtg gtcaagaccg ccgccgcgaa
     2461 gatggatatc caggacgcaa agatcctggt ctccggcggc cgcggcatgt gcagcgccga
     2521 gaactttcat ctgctgcagg agctggccga cgtgctcggc ggcaccgtga gctgcagccg
     2581 cgcggccgtg gacgcgggct gggctgaaaa ggatatgcag gtcggccaga ccggcaagac
     2641 cgtgcgcccg aacctgtaca tcgcctgcgg catctccggc gccatccagc atctggccgg
     2701 catggaggat tccgacgtga tcgtcgccat caacaaggac gagaccgccc cgatctttga
     2761 ggtcgcggac tacggcatcg tgggcgacgc gctgcagatt gtgccgctgc tcaccgaggc
     2821 cctcaagcgg gagatcgtcg ccaagggctg agtgtgctga gcgcatccgc agtgaaaaga
     2881 gtgcctcatc gcccgcagcg cagcaaagac ctgggagaat gcgcagcatt ctcgcatgga
     2941 tgcgcacaaa aaacatagcg aagagcaaag catcttcgca tcctaatgcc tgtctctcgg
     3001 acagtgttcc gttccatttt aagcacccac cccgccgcgc gaagcaccgc gcggcggggt
     3061 gggtgcgttc ttttttgagg agaaaaaacc ttctgtgagg ggagaaaaac tgccgcccgc
     3121 atcggggtaa ccaacaaatt ttttagccca ccggattcaa aacaagaggg tggcagaaaa
     3181 aggtctacaa aaataaaatt taaatttgtg agaatcgaaa aaaatcataa aatgtggttt
     3241 tcaaaaaaga aaataaagag tattatgaca attgggatgg tatgcttttt gcgcatttga
     3301 aaaattggaa tttagatgga taag
//
LOCUS       DBMDYA010000284         3318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6500_length_3318_cov_1.478085, whole genome shotgun sequence.
ACCESSION   DBMDYA010000284 DBMDYA010000000
VERSION     DBMDYA010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6500_length_3318_cov_1.478085"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(121..1047)
                     /locus_tag="ACZPFK_07730"
     CDS             complement(121..1047)
                     /locus_tag="ACZPFK_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1406238.1"
                     /translation="MACVIALDIGGTSVKAGLVSESGTLFERARFEVQEDGLTGAVRT
                     AARVMALRARQTGAAPCGVAAAATGQIDSRCGQVVGTNGAIAGWEGVHLTSLLQQETG
                     LARVCVANDANCAAYLEATRGAGRGRDEVLLVTLGTGIGGGIVQGGKLLGGARGLAGE
                     IGHICVDPRGRRCTCGRRGCWERYASVGALVRDAAAAGGAAVPRNGKEVFALEQAGNA
                     AVHAVMERYFDFLALGLANLIHIFNPDAVILGGGVSAEGERLLGRVRPRLEKSVMPQF
                     LQGLALCTAAFQNDAGLLGAAALFFGGGMGEK"
     gene            complement(1048..1740)
                     /locus_tag="ACZPFK_07735"
     CDS             complement(1048..1740)
                     /locus_tag="ACZPFK_07735"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIR1406239.1"
                     /translation="MKKQQALDSLKGGLIVSCQALRGEPLYLEQGGVMPRIAAAMQGV
                     GAAGIRCNSERDIREIRAAVELPLIGLIKQRYEGYFPYITPTLKEVEAVVRAGADIVA
                     VDATASMHPGGLRADKFIRQIKEKYDILVMADISTEEEAQAALRAGADLVGTTMAGYT
                     PYTVAGDGPDFELLQRLVRCLACPVVAEGRVHTPAQAKRALQLGAFAVVVGGAITRPQ
                     EIAQRFIKEVFA"
     gene            complement(1749..3266)
                     /locus_tag="ACZPFK_07740"
     CDS             complement(1749..3266)
                     /locus_tag="ACZPFK_07740"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1406240.1"
                     /translation="MNVMQIEHVTKTFPGVRALDDVTFAIRKGEILGIVGENGAGKST
                     LMQILSGVHTHGTYAGRVLVEGKELQCRTTRDAEACGIAMIYQELNFHLDTSVAENLF
                     MGDWLTGPLGEVRWKEIYRQSAALLAEVGLEEIDAQMPMRSLSKAQQQMVAIAHALRK
                     NPKVLIMDEPTSTLHKKEVGRLFETIHRLNGEGITVVLITHKMEEVFGNCTRLVVMRN
                     GRFVAEHDIGAVTVDEVISEMVGHSFHTYTEEASPMRAPGEEVLRVEQFTVAHPLNKN
                     KNILEGISFSLHRGEVLALEGLVGAGKTELLSAIFGKGGLRGGKLYLDRRAVRITHQA
                     DAIANGIALVTEDRREDGFVDILSILRNIALPNLHRLSRFGVLNRRREQQEAQRYAAR
                     LAVKAPSLEVPVGNLSGGNQQKVVLAKWLMSQPKVLLLDDPTRGIDVGAKEEIYKIIH
                     ELAESGMAILITSSERSELLRLSDRMIILCDGRISGQLEKGEYDEDLIVNLSVKQ"
BASE COUNT          560 a         1191 c          939 g          628 t
ORIGIN      
        1 gaccacatcc gccaccagct tttctgaatt tgtcagcgtc ttgtagcggc tgctgatggt
       61 ggagagcaca ttgcccacct ccacgatgtc tttcatccct ttattccccg caatctcttt
      121 ttatttttcg cccataccac cgccgaaaaa cagggccgca gcgcccaata gcccggcatc
      181 gttttgaaat gccgcagtgc aaagcgccag gccctgcaaa aactggggca tgaccgattt
      241 ttccagccga gggcgcaccc ggcccagcag gcgctcgccc tcagccgaga cgccgccacc
      301 caggatcacc gcatccgggt tgaagatgtg gatgaggttt gcaagcccca gggcaagaaa
      361 gtcgaaatag cgctccatca ccgcatggac ggcggcgttg cccgcctgct ccagcgcaaa
      421 gacctccttg ccgtttcggg ggacggccgc gcccccggcc gccgcggcgt cgcgcaccag
      481 cgcgccgacg ctggcgtacc gctcccaaca tcccctgcgg ccgcaggtgc agcgcctgcc
      541 ccgcgggtcg acgcagatgt ggccgatctc gccggccagc ccccgcgcgc cgcccagcag
      601 cttgccgccc tgcacgatgc cgccgccgat gccggtgccc aaggtgacca gcagcacctc
      661 gtcccgcccg cggcccgcgc cccgggtggc ctcaaggtaa gcggcgcagt tggcgtcgtt
      721 ggccacgcag acccgcgcca aaccggtctc ctgctgcagc aggctcgtca gatggacgcc
      781 ctcccagccg gcgatggcgc cgttggtccc caccacctgg ccgcaacggc tgtcgatctg
      841 gccggtggcc gcggcggcga cgccgcaggg cgctgcgccc gtctgccgcg cgcgcagcgc
      901 catcacccgc gccgctgtcc gcaccgcgcc ggtcagcccg tcctcttgca cctcgaaccg
      961 ggcgcgttca aaaagagtcc cgctctcaga gaccaggccc gcctttacgc ttgtgccgcc
     1021 gatatccagt gcgatcacac aggccattta tgcaaacacc tctttgataa agcgctgggc
     1081 gatctcctgc ggacgggtga ttgccccgcc gacgaccacc gcaaacgccc ccaactgcag
     1141 cgcccgcttt gcctgcgcgg gggtgtgcac ccgcccctcg gccacgaccg ggcaggccag
     1201 acaccgcacc agcctttgca aaagctcaaa atccgggccg tcgcccgcca ccgtataggg
     1261 cgtataaccc gccatcgtgg tgcccaccag atcagcgcct gcccgcaggg ctgcctgcgc
     1321 ctcctcctcc gtggaaatat ccgccatcac cagtatatca tatttttctt tgatctgccg
     1381 gataaacttg tccgcccgca gcccgcccgg gtgcattgaa gcagtggcgt ccaccgccac
     1441 gatatccgcc cccgcccgca ccacggcctc cacctctttc agggtggggg tgatataagg
     1501 gaaatagccc tcatatctct gcttgatgag gccgatgagc ggcagctcca ccgccgcccg
     1561 gatctcgcgg atatcgcgct cggaattgca gcggatgccc gccgccccga caccctgcat
     1621 cgcggccgct atccggggca tgacgccgcc ctgctccagg tagaggggtt ctccccgaag
     1681 agcctggcag gacacgatca gcccgccttt gaggctgtcg agcgcttgct gttttttcat
     1741 atttctcctc actgctttac agaaagattg acgatcaggt cctcgtcgta ttcgcctttt
     1801 tccagctgcc cgctgatccg cccgtcgcac aggataatca tgcggtcgga aaggcgcaaa
     1861 agctccgacc gctccgagga ggtgatcagg atcgccatcc cgctttccgc cagctcatgg
     1921 atgatcttgt agatctcctc tttggcgccc acgtcgatac cgcgggtggg gtcgtccagc
     1981 agcagcacct ttggctggga catcagccat tttgcgagca ccaccttttg ctgattgccg
     2041 ccggacaggt tccccaccgg cacctccagc gagggagcct tgacggcgag gcgggcggca
     2101 tagcgctgcg cctcctgctg ctccctgcgc cggttgagca cgccgaagcg gctgagccgg
     2161 tgcaggttgg gcaaggcgat gttgcgcagg atgctcagga tatccacaaa gccgtcctcg
     2221 cgccggtcct ccgtgaccag ggcgatgccg ttggcaatcg cgtccgcctg atgggtgatc
     2281 ctgaccgctc tcctgtccag atacagcttg ccgccgcgca gcccgccctt gccgaaaata
     2341 gcgctcaaca gctccgtctt gcccgcgccc accagcccct ctaaggccag cacctcgccg
     2401 cggtgcagag aaaaactgat cccctccaaa atatttttgt ttttgttgag cggatgggcg
     2461 acggtgaact gctccacccg cagcacctcc tcgcccggcg cgcgcattgg agacgcttct
     2521 tccgtataag tgtgaaagga gtgccccacc atctcggaga tcacctcgtc caccgtgacc
     2581 gccccgatgt cgtgctcggc gacgaagcgg ccgttgcgca tcaccaccag acgggtgcag
     2641 ttgccgaaca cctcctccat cttgtgggtg atgagcacca cggtgatccc ctccccgttc
     2701 aggcggtgga tggtctcaaa cagccgcccc acctcttttt tgtgaagggt ggaggtgggc
     2761 tcgtccatga tcagcacttt tgggtttttt ctcagggcat gggcgatagc caccatctgc
     2821 tgctgggctt tggataggct gcgcatgggc atctgcgcgt cgatctcctc cagccccacc
     2881 tcggccagca gcgccgcgga ctggcggtag atctctttcc agcgcacctc ccccagcggg
     2941 ccggtgagcc aatctcccat gaacaggttt tcggcgacgc tggtgtcgag atggaagttg
     3001 agctcctgat agatcatggc gataccgcac gcctccgcat cacgggtggt gcggcactgc
     3061 agctctttgc cctccaccag cacccggccc gcataggtac cgtgggtgtg cacgccgctc
     3121 aggatctgca tcagcgtcga ttttccggcc ccgttctccc ccacgatgcc cagaatctcc
     3181 cccttgcgga tggcaaaggt gacgtcgtcc agcgcgcgca caccgggaaa ggtcttggtc
     3241 acatgctcga tctgcatcac attcatgctt tgacacctcc cggagcctca ggcctgtttt
     3301 ttccttttaa agcggtcc
//
LOCUS       DBMDYA010000285         3312 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6515_length_3312_cov_1.317347, whole genome shotgun sequence.
ACCESSION   DBMDYA010000285 DBMDYA010000000
VERSION     DBMDYA010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6515_length_3312_cov_1.317347"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="ACZPFK_07745"
     CDS             complement(<1..366)
                     /locus_tag="ACZPFK_07745"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR1406241.1"
                     /translation="MNERDDDYFMGLALEQARRAAALGEVPVGAVVVRDGRVVGAGHN
                     RRETGRNALAHAELAAIDAACRELGGWRLWQCDLYVTLEPCPMCTGAIINSRLVRVVY
                     GAGDPRAGCCGSLIDLTALP"
     gene            complement(363..1319)
                     /locus_tag="ACZPFK_07750"
     CDS             complement(363..1319)
                     /locus_tag="ACZPFK_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1406242.1"
                     /translation="MAQDRPRRDNLYYSANLIGMAVGLYLLLGQLLQAALSAALGANL
                     PGAGAANPAGIPVWAAMLLNCLATLVNLGAPILCLRAWAKPLGLPRRPLRPPARPVVL
                     LGIPLFLCYTTLCSALSNLVRILLGFGGYTPPAATHLPDSTGGLALAFVAICVLPAVL
                     EELFFRGAIQGMLAPYGDWFSIVVTSALFALLHSDLSQLPAIFALSLFLGYVAAATKN
                     VVNSILLHFVNNVSSFLLLWSQQQMDGTNAVGLSAILFTLYFGAGLLALAALIRRGGA
                     KKLPRYPTRKNRMGRTERILSAPVFVFMLLILILIAILEYFR"
     gene            1978..3225
                     /locus_tag="ACZPFK_07755"
     CDS             1978..3225
                     /locus_tag="ACZPFK_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1406243.1"
                     /translation="MSPNLLIALLAVPGLFLAAYGLYFAGIALFGLRRRAHYEPAAPK
                     TRFAVVIAARNEEAVIGNLVDSLLRQDYPRELYDVIVAPNNCTDGTEAVARAHGARIF
                     RHEGRVKSKGEVLRQVVEKVVLAEGYDAMCVFDADNLVDAAFLSRMNDARQAGAGAAQ
                     GFRDSKNPHQSAVSGCYSICYWMLSHFYNNARAVLGLSALVNGSGFMVTAALLRRLGG
                     WNTRTMTEDYEFSAQCVLAGERVFFVPDAVIYDEQPLTFLQSWKQRRRWTTGSLQGLE
                     LYGGRLFAHAVSEHDMVSFDMYLTFLSPIIQVVSAVAGALAAGLMIWQGRLEPVHLLI
                     AVAAAAGSVLASAVGCAVVALAAVLMKKTPLPGMAKSILSFWLFMGSWMVITFVSMVR
                     RQKNWDPIAHTSALTIDEVGRTA"
BASE COUNT          589 a         1066 c         1187 g          470 t
ORIGIN      
        1 aggcagggcg gtcaggtcga tcaggctgcc gcagcagccc gcgcgggggt cgccggcgcc
       61 gtagaccacc cgcaccaggc ggctgttgat gatggcgccc gtgcacatgg ggcagggctc
      121 cagcgtcaca tagaggtcgc actgccacag ccgccagccg cccagctctc tgcaggcggc
      181 gtcgatggcg gccagctcgg cgtgggccag ggcgttgcgg ccggtctcgc gccggttgtg
      241 gcccgcgccc accacccggc cgtcgcgcac caccaccgcg cccaccggca cctcgcccag
      301 ggcggcggcg cggcgggcct gctccagcgc aaggcccata aaatagtcgt cgtctctctc
      361 gttcatctga aatactccaa tatcgcgatc aggatcagga tcagcagcat aaagacaaag
      421 accggggccg agaggatgcg ctcggtgcgg cccatgcggt ttttgcgggt ggggtagcgg
      481 ggcagctttt tcgcgccgcc gcggcggatc agcgccgcca gcgccagcag cccggcccca
      541 aagtaaaggg tgaacaaaat ggccgacagg cccacggcgt tggtgccgtc catctgctgc
      601 tgcgaccaga gcagcaaaaa gctggacacg ttgttcacaa agtgcagcag gatggagttg
      661 accacgtttt tggtggccgc cgccacatag cccagaaaga gcgagagggc aaagatggcg
      721 ggcagctggg acaggtcgga gtgcagcagc gcaaagaggg ccgaggtgac gacgatcgaa
      781 aaccagtcgc cgtagggggc cagcatgccc tggattgcgc cgcggaaaaa cagctcctcc
      841 agcacggcgg gcagcacgca gatggccaca aaggccagcg ccaggccgcc cgtgctgtcc
      901 ggcaggtggg tggccgcggg cggggtgtag ccgccgaagc ccagcaggat gcgcacgagg
      961 ttggacaggg cgctgcacag ggtggtgtag cagagaaaaa gcgggatgcc cagcagcacc
     1021 accggccgcg cgggcggccg cagcggccgc cggggcaggc ccagcggctt tgcccaggcg
     1081 cgcaggcaga ggatgggcgc ccccaggttg acgagggtgg ccaggcagtt cagcagcatc
     1141 gccgcccaga ccgggatgcc ggccgggttg gccgcgcccg cgccgggcag gttggcgccc
     1201 agggccgccg agagggcggc ctgtaaaagc tggcccagca gcaggtacag ccccaccgcc
     1261 atgccgatca ggttggccga atagtacagg ttgtccctgc gcggtctgtc ctgtgccatc
     1321 ctgatcccct ccctttttcg ccgcgggcgt ttcgtcctgc cctctatagt aatagaaacg
     1381 gaaaaaaatt acaatatgcg ccgcccggcc gcgcccggtc cggcggtggc cggcagcccc
     1441 ggggaaaggg ggagcggccc ggggaaaggg cggccaacgc gggagaaaga cggaaaacgg
     1501 ccccacgggg agatgtgcgg ccgcaaggag agggaggaac gaccccggaa agagggaagt
     1561 ggccccgggg aggggacggc ggccctgagg agaggagcga acaccgctgg agagagggcg
     1621 gcggcctcga agaaagagaa gcggcggcca ccgccctctg ccgcgaaaaa acgggcggag
     1681 gaaaggggct ccgggccgcc gcagccacgg tcccaaagcg ggcagaaagg cccgcgcggc
     1741 ggggcgtttt gcccctctgt cacggcccgg gcggggcggc cggtgaaaat ttcctgtaaa
     1801 aaaatcgccg ggatttgaag agttttgcac ttgcgggtcg tttcaatgct aggatgacaa
     1861 aacggggacg agccccggcg gcgcgcggcc ggtgtctcgg acgcggcgcc cgccccgggc
     1921 ccgccccgac aaaataaccc cctctcccca gcggagagaa aaaaggagct gactgctatg
     1981 tcgccgaatc tgttgatcgc actgctggcc gtgcccggcc tgtttttggc agcgtacggc
     2041 ctgtattttg cgggcatcgc cctgttcggg ctgcgccgcc gcgcccacta cgagcccgcc
     2101 gccccgaaga cccgcttcgc ggtggtgatc gccgcccgca acgaggaggc cgtcatcggc
     2161 aacctggtgg acagcctgct gcggcaggac tacccgcgcg agctgtacga cgtgatcgtc
     2221 gcgcccaaca actgcaccga cggcaccgag gccgtggccc gggcccacgg ggcccgcatc
     2281 ttccgccacg aggggcgggt gaagagcaag ggcgaggtgc tgcgccaggt ggtggaaaaa
     2341 gtggtgctgg ccgaggggta cgacgccatg tgcgtgtttg acgccgacaa cctggtggac
     2401 gccgctttcc tctcccgcat gaacgatgcc cgtcaggcgg gcgcgggcgc ggcgcagggc
     2461 ttccgggaca gcaaaaaccc gcatcagagc gcggtgtccg gctgctactc gatctgctat
     2521 tggatgctga gccattttta caacaacgcc cgcgccgtgc tgggcctgtc ggccctggtg
     2581 aacggcagcg gctttatggt cacggccgcg ctgctgcgcc gcctgggcgg ctggaacacc
     2641 cgcaccatga ccgaggacta tgagttcagc gcccagtgcg tgctggcggg cgagcgggtc
     2701 ttttttgtgc cggacgccgt catctacgac gagcagccgc tgacctttct ccagtcgtgg
     2761 aagcagcgcc gccgctggac caccggcagc ctgcaggggc ttgagctcta cggcggccgc
     2821 ctgttcgccc acgcggtgtc cgagcacgac atggtgagct ttgacatgta cctgaccttc
     2881 ctctcgccga tcatccaggt ggtgtcggcc gtggccggcg cgctggccgc ggggctgatg
     2941 atctggcagg gccggctgga gccggtgcac ctgctgatcg cggtggccgc ggcggcgggc
     3001 tcggtgctgg ccagcgccgt gggctgcgcg gtggtggccc tggccgccgt tctgatgaaa
     3061 aagacgccgc tgcccggcat ggccaagagc atcctctcgt tttggctgtt catgggcagc
     3121 tggatggtaa tcacctttgt cagcatggtg cgccgccaga agaattggga ccccatcgcc
     3181 cacaccagcg ccctgaccat cgacgaggtg gggcgcacgg cctgagccgc ggcgccgccg
     3241 gtttggcgcg aagagaaaaa aagagaagca ggaccgtcct catggcggcc ctgctttttt
     3301 gcgccccgaa aa
//
LOCUS       DBMDYA010000286         3303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6533_length_3303_cov_1.350718, whole genome shotgun sequence.
ACCESSION   DBMDYA010000286 DBMDYA010000000
VERSION     DBMDYA010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6533_length_3303_cov_1.350718"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(3..1835)
                     /locus_tag="ACZPFK_07760"
     CDS             complement(3..1835)
                     /locus_tag="ACZPFK_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR1406244.1"
                     /translation="MKVQKPGRYTGGEPGSIYKDPAEMECRFAFCFPDTYEVGMSFLG
                     MKVLYEILNARQNIWCERAFMPWVDMLEQMRQREIPLFSIESKTPLSQFDIVGFTLQY
                     ELSYSNILAMLDLSGIPFYAKDRGQSCPLVVAGGPCTCNAEPVADFFDAIMLGEGEEQ
                     LPVLCDAVIDAKRRGLSRAELLLNLSKIKGVYVPSLYSVQYNGDGTVKHVTAHKGAPR
                     VVEKAILPNFRHQPLPTHFTVPMVGAVHDRAMVEVLRGCIRGCRFCQAGFIYRPMRER
                     DADMLNNAARDLCRNTGYEEVSLTSLSTSDHSQLEPLLDKLLTWTEGEHVNLALPSLR
                     IDNFSESLVEKTTRVRKSGLTFAPEAGTQRLRDVINKNVTMDEVLKSCTLAFQNGYTA
                     VKLYFMMGLPTETLEDIKGIADTAQAVVDLYYSLPQRPKGKGVTVSISVACFVPKPFT
                     PFQFAAQDTMEQLREKQQYLLSCVRTKKISVSYHDARVSFLEAVFAKGNRRLSRVLVE
                     AYKSGCCFDGWAEHFKFDTWVECFKKCGIDMAFFANRPIPLEEITPWEHFSYGVDKRF
                     LIEEYQRAQRAQTTMPCNRKCSGCGANKLMGGGKCVANDQSVVL"
     gene            complement(2701..3033)
                     /locus_tag="ACZPFK_07765"
     CDS             complement(2701..3033)
                     /locus_tag="ACZPFK_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019498.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1406245.1"
                     /translation="MCRSSRGGSVPIANVTFEPGCRNNWHIHRAERQGGQILLCTMGR
                     GWYQEWGKAARELHPGDVVEIPAGVKHWHGAAKDSWFAHLAVEVPGENAANEWLEPVD
                     KAQYDALE"
     gene            complement(2981..3118)
                     /locus_tag="ACZPFK_07770"
     CDS             complement(2981..3118)
                     /locus_tag="ACZPFK_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406246.1"
                     /translation="MKKKPESGLLFPLGGPNEAYARFFTGQSYLQKLTGGKRPHRQRH
                     L"
BASE COUNT          595 a         1064 c          988 g          656 t
ORIGIN      
        1 gcttagagaa ccacactctg atcgtttgca acgcatttcc ctccccccat cagcttgttg
       61 gcgccgcagc cgctgcactt gcggttgcag ggcatcgtcg tctgggcgcg ctgggcgcgc
      121 tggtactcct cgatgagaaa acgcttgtcc acgccgtagc tgaagtgctc ccagggggtg
      181 atctcctcaa gagggatggg ccggttggca aaaaaagcca tgtcgatgcc gcattttttg
      241 aagcactcca cccaggtgtc gaacttgaag tgctccgccc agccgtcaaa gcagcagccg
      301 cttttgtagg cctcgaccag cacccgggag agcctgcggt tgcccttggc aaacacagcc
      361 tccaaaaagc tgacgcgggc gtcgtggtag ctgaccgata tcttttttgt gcgcacacag
      421 ctgagcaggt attgctgctt ttcccgcagc tgttccatgg tgtcctgggc ggcgaactgg
      481 aacggcgtaa agggcttcgg cacaaaacag gccacgctga tggagactgt gacccccttg
      541 cccttgggcc gctggggcag gctgtaatac aggtccacca ccgcctgggc cgtgtccgcg
      601 atgcccttga tgtcctccaa cgtctcggtg ggcaggccca tcatgaaata gagcttgacg
      661 gcggtgtagc cgttttgaaa ggcgagcgtg cagcttttga gcacctcgtc catggtgacg
      721 ttcttgttga tgacgtcgcg cagccgctgg gtgcccgcct ccggcgcaaa ggtcaggccg
      781 ctcttgcgca cccgggtggt cttttccacc aggctctcgg agaaattgtc gatgcgcagc
      841 gagggcagcg ccaggttgac gtgctcgccc tccgtccagg tgagcagctt gtccagcagc
      901 ggctccagct ggctgtggtc gctggtggac agcgaggtca gcgagacctc ctcgtagccg
      961 gtgttgcggc acagatcgcg cgccgcgttg ttgagcatgt cggcgtcccg ctcgcgcatg
     1021 gggcgataga taaagcccgc ctggcagaac cggcagccgc ggatgcagcc gcgcagcacc
     1081 tccaccatgg ccctgtcgtg caccgccccc accatcggca ccgtgaagtg ggtgggcagc
     1141 ggctgatgac ggaaattggg caaaatcgcc ttttccacca cccgcggcgc gcccttgtgc
     1201 gcagtgacgt gctttaccgt gccgtcgccg ttgtactgca cagaatagag cgagggcacg
     1261 taaacgccct tgatcttgga caggttgagc agcagctccg ccctggagag gccgcgccgc
     1321 ttggcgtcga tgacggcgtc gcagagcacc ggcagctgct cttccccctc ccccagcatg
     1381 atggcgtcaa aaaaatccgc caccggctcg gcgttgcagg tgcaggggcc cccggcaacg
     1441 acgagcgggc agctttgccc gcggtccttt gcatagaacg ggatgcccga gagatccagc
     1501 atggccagga tgttggagta ggacagctca tactgcagcg tgaagccgac gatatcaaat
     1561 tgggacagcg gtgtcttgct ctcgatggaa aacagcggga tctcccgctg ccgcatctgc
     1621 tccagcatat ccacccaggg catgaacgcc cgctcgcacc agatgttttg ccgcgcgttc
     1681 aaaatctcat agagcacttt catgcccaaa aaagacatgc ccacctcata ggtgtcgggg
     1741 aagcaaaaag caaaccggca ctccatctcg gccggatcct tgtaaatact gccgggttcc
     1801 cctccggtat accggcccgg tttttggact ttcatcaaaa cctgttcaag ctgctttggg
     1861 atagccacca gatcacctct gccctcgtat aataaaccgc acggctgtgc gccgtcgtac
     1921 cagtccgccg ctctccgggg aatctccccc gccaaaggtt ggccgcgccg ccgcgcttcc
     1981 ccggtgggaa gcagcgcggg gcgttttcgt ccctcgcacc cgggctccga tcaccgccga
     2041 cgggcccttg ccggtaacct ttcgtgatgg tacggcaaaa gattgcctgt cgtgagcggc
     2101 gctgggggaa cggccaaacg cccggcaaaa acagcgcagg gctgtgccgc ggctgcttta
     2161 ttatagcata cctccccggt tccgacaaat ggcggcgcaa aaaaatgcag ggcccggccc
     2221 tttgtcgctg cccctctgca gcccgctttc ttcgtctgcg cgctttcctc ccccgagctc
     2281 cgtgcggcgg gcagcgggga gcgcgcccgt cgagaggcgc ggcccgctct tgcggacagg
     2341 aaaaagcacc caaagtgagt cccggatcag aatggtggta aaagtaatat tctccccatt
     2401 gtgcaataca gacagaggcc ggggccgctc ttttcgggac ggccccggtc tctgtgcaaa
     2461 aaagacaatg cgcttgcttt tattaaaata tccgcccccg gccactgggc cccgttgcct
     2521 tgcgtccctg gccgcagcat acagtttcgg cttttcgccg ttgaaagggc gctctcggcg
     2581 gcggccgcag cagccccgtg tccgccgagg cgggccgcgg gctggggaca ttttggagtg
     2641 aaaagatcaa gatcaccggg gggaagtgtc gttcgggccg caggggccgc gcgccccctt
     2701 tcactccaaa gcgtcgtatt gcgccttgtc caccggctcc agccactcgt tggcggcgtt
     2761 ttctcccggc acctcgaccg cgaggtgggc aaaccagctg tcctttgcag cgccgtgcca
     2821 gtgcttgacc ccggcgggga tctccaccac gtcgcccgga tgcagctccc gcgccgcttt
     2881 gccccactcc tgataccagc cccggcccat cgtgcacagc aaaatctgac caccctgccg
     2941 ctctgcgcgg tggatatgcc agttgttgcg gcacccaggc tcaaaggtga cgttggcgat
     3001 ggggacgctt cccccccgtg agcttctgca gatagctctg cccggtaaaa aaccgggcgt
     3061 atgcctcgtt ggggccgccg agcggaaaaa gcaagccgct ttcaggtttt ttcttcatct
     3121 gtctccgcct ctcttttctt tagatgacgc gcgcggcgtc gggattaccc agttgttttc
     3181 tccgccgccc tgtcgatgca gctgatggcg ttcaggctgc ggggataacc gatatagggc
     3241 aggcactggt aaaccaccgc gaccaaaaag tctctgtcgt tgcccaggtt catattgccg
     3301 cac
//
LOCUS       DBMDYA010000287         3290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6571_length_3290_cov_1.518031, whole genome shotgun sequence.
ACCESSION   DBMDYA010000287 DBMDYA010000000
VERSION     DBMDYA010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_3290_cov_1.518031"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            24..1334
                     /locus_tag="ACZPFK_07775"
     CDS             24..1334
                     /locus_tag="ACZPFK_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1406247.1"
                     /translation="MKSESPIWDAKKLGIPKMLLLGLQHTFAMFGATVLVPIITGLNV
                     STTLLFAGLGTLLFHLITKGKVPAFLGSSFAFLGGYAMVAPKLADGSPNAELLPYACG
                     GVVAAGLVYVALSALIKAFGVKKVMRFFPPVVTGPIIISIGLILAPTAVTSASEDWLL
                     AFVALAIIVVCNIWGKGMVKIIPILLGIVGSYLLAVCLGRVDFTAVKDAQAFALAPIT
                     MAKFSISAVFTIMPIALATMMEHIGDIAAIGATTGKNYISNPGLHRTLLGDGLATSLA
                     GLFGGPANTTYGENTGVLALTRVYDPRVMELAAVFAVALSFIPKFGELIHSIPNAIIG
                     GVSFILYGMISAIGVRNVVENKVDLTKSRNLIIAAVILVSALGFNSIGGLSFTITSDI
                     TISLSGLAIAAILGITLNAVLPGNDYSFDDEKPDDGKTSVSFKV"
     gene            complement(1771..3096)
                     /gene="clpX"
                     /locus_tag="ACZPFK_07780"
     CDS             complement(1771..3096)
                     /gene="clpX"
                     /locus_tag="ACZPFK_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1406248.1"
                     /translation="MANKEKDITCSFCGKSQDAVERMIIGPGVNICSECIELCGTLLS
                     EEGVRPKPSQHAPQRIARPGAKPAPAAPQNDFKILTPQEIKEGLDQYVIGQDDAKMVL
                     AVSVYNHYKRILAKDYSNDVELQKSNVLLVGPSGVGKTYLAQNLARMLKVPFAIADAT
                     TLTEAGYVGEDVENILLKLIQAADFDVERAEKGIIYIDEIDKITRKSENPSITRDVGG
                     EGVQQALLKILEGTVANVPPQGGRKHPQQEFIQINTKNILFICGGAFDGLAKHIQKRT
                     DSSGLGFGSQLKEPKKNTDDAKMLRLVEPHDLVKFGLIPELVGRLPIVTVLDALDEGA
                     LVRILKEPKNSIVKQYKALFALDNVELDITDEALHEMAKKAIKLKSGARGLRSIVEQT
                     LMETMYEVPSDPTIIQVTVDADAVLEGKPKLEYGAVRKRYKNTVNTLIH"
     gene            complement(3114..>3290)
                     /locus_tag="ACZPFK_07785"
     CDS             complement(3114..>3290)
                     /locus_tag="ACZPFK_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR1406249.1"
                     /translation="IKIHADHIIRIRRRMNEMYAQYTGQPLERIEADTERDNFLTAQQ
                     AADYGLIDAVTTKR"
BASE COUNT          589 a         1027 c          910 g          764 t
ORIGIN      
        1 taaactgcga ggaggaatgt atcatgaaat ctgaaagccc gatctgggac gccaaaaagc
       61 tcggcatccc aaaaatgctg ctgctcggcc tgcagcacac ctttgccatg ttcggcgcca
      121 cggtgctggt acccatcatc accggcctga atgtctccac cacccttttg ttcgcaggcc
      181 tcggcaccct gctgttccac ctgatcacca agggcaaggt gcccgccttt ttgggctcct
      241 cctttgcgtt tttgggcggc tacgccatgg tggcgcccaa gctggccgac ggctcgccca
      301 acgccgagct gctgccctac gcctgcggcg gcgtggtggc cgcgggcctg gtctatgtgg
      361 ccctctcggc gctgatcaag gctttcggcg tcaaaaaggt catgcgcttc tttccgcccg
      421 tggtgaccgg tcccatcatc atctccatcg gcctcatcct cgcccctacc gcggtcacca
      481 gcgccagcga ggactggctg ctggcctttg ttgcgctggc cattatcgtg gtctgcaaca
      541 tctggggcaa ggggatggtc aagatcatcc ccatcctgct gggcatcgtc ggctcttatc
      601 tgctggccgt ctgcctgggc cgcgttgact ttaccgcggt caaagacgcg caggcctttg
      661 ccctggcacc tatcaccatg gcgaaattca gcatcagcgc cgtctttacc atcatgccca
      721 tcgccctcgc gaccatgatg gagcacatcg gcgacatcgc cgccatcggg gcgaccaccg
      781 gcaaaaatta tatcagcaac cccggcctgc accgcaccct gctgggtgac ggtctggcca
      841 ccagcctggc gggcctgttc ggcggccccg ccaacaccac ctacggcgag aataccggcg
      901 tcttggccct gacccgcgtc tatgatccgc gcgtgatgga gcttgccgcc gtctttgccg
      961 tggccctctc tttcatcccc aaattcggcg agctcatcca ctccatcccc aacgccatca
     1021 tcggcggcgt cagctttatc ctttacggca tgatcagcgc catcggtgtg cgcaatgtgg
     1081 tggagaacaa ggtcgacctg accaaatccc gcaacctgat cattgcggcg gtgatcctgg
     1141 taagcgccct gggcttcaac agtatcggcg gcctctcctt taccatcacc tccgatatca
     1201 ccatctcgct ctcaggcctc gccattgcgg cgatcctggg catcacgctc aacgcggtgc
     1261 tgcccggcaa tgattacagc tttgacgacg agaagccgga cgacgggaaa acgtccgtct
     1321 cgttcaaggt ctaagctgta tgtcctaccc tctcatcttt tcaaaagcgc aattgaaaat
     1381 gagtcggcgt ggacggcgct catgtggatc tcacgacaaa cgcagccgca gccgtcccca
     1441 tggttagaag caagttcaat ttttaaaagc gacctctcat gtgagccgat tttggcttgg
     1501 atgagaggtc ttcttttggt tctggaggct cttcaaagcc actatcgccg cctcaccccg
     1561 gggtgaaaaa attggattgg gccctaaaaa aagaacggct ccttcaggct gatttgggcg
     1621 ggggaagaaa aaatcttttt tgccccagaa gcggatcaga acctttttcg tgtccccttg
     1681 ccccagggtt aaagatggaa aaggcgcaca aaaaactgcg gcggagccag cgctccgccg
     1741 cagtttgcta tgtctaaaag aaaaaagtgc tcaatggatc agggtgttga cggtgttttt
     1801 ataccgcttg cgcaccgcgc cgtattccag cttgggcttg ccctccagca ccgcgtccgc
     1861 atccacagtc acctggatga tggtggggtc gctgggcacc tcatacatgg tctccatcag
     1921 cgtctgctcc acgatgctgc gcaacccgcg cgcgccgctc ttgagcttga tggccttttt
     1981 cgccatctca tgcagcgcct cgtcggtgat gtccagctcc acattgtcca gcgcaaaaag
     2041 agccttgtac tgctttacga tggagttctt cggctctttg aggatgcgca ccaacgcccc
     2101 ctcatccagc gcgtccagca ccgtgacgat gggcagacgg cccaccagtt cggggatgag
     2161 gccgaacttg accagatcgt ggggttccac cagacgcagc attttcgcgt cgtcagtatt
     2221 ctttttcggc tctttcagct ggctgccgaa ccctaggcca gagctgtcgg tgcgcttttg
     2281 gatgtgtttt gccagcccgt cgaaagcgcc gccgcagatg aaaaggatgt ttttggtgtt
     2341 gatctgaatg aattcctgct gggggtgctt gcggccgccc tggggaggca cattggcgac
     2401 cgtgccctcc aaaatcttca gcagcgcctg ctgtacgccc tcgccgccca catcgcgggt
     2461 gatggagggg ttttcgctct tgcgggtgat tttgtcgatc tcatcgatat agatgatgcc
     2521 tttttccgcc cgctccacat caaaatcggc cgcctggatg agcttgagca ggatgttctc
     2581 cacgtcctcg cccacatagc ccgcctcggt gagggtggtg gcgtccgcaa tcgcaaaggg
     2641 tactttcagc atacgggcca ggttttgagc cagataggtc ttacccacgc ccgagggccc
     2701 caccagcagc acattgctct tttgcagctc tacatcgttg ctgtagtcct tggcgaggat
     2761 gcgcttatag tggttgtaga ccgagacggc cagcaccatt tttgcgtcgt cctggccgat
     2821 gacgtattgg tccagcccct ctttgatctc ctgcggggtc aggatcttga aatcgttctg
     2881 cggcgccgcc ggcgcgggct ttgccccggg ccgggcgatg cgctgcggtg catgctgcga
     2941 cggcttgggc cgcaccccct cctctgaaag aagggtgccg cacagctcga tgcactcgct
     3001 gcagatattg acgccggggc cgatgatcat gcgctcgacc gcgtcctggc ttttgccgca
     3061 aaagctacag gtgatgtcct tctctttatt tgccatgttt caccaactta cttttatcgc
     3121 tttgtggtca cggcgtcgat caggccataa tccgccgcct gctgtgccgt cagaaaattg
     3181 tcgcgctcgg tgtccgcctc gatgcgctcc agcggctgtc cggtgtactg ggcgtacatc
     3241 tcgttcatcc tgcggcggat gcggatgatg tggtcggcgt ggattttgat
//
LOCUS       DBMDYA010000288         3261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6635_length_3261_cov_1.129430, whole genome shotgun sequence.
ACCESSION   DBMDYA010000288 DBMDYA010000000
VERSION     DBMDYA010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_3261_cov_1.129430"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /gene="pyrG"
                     /locus_tag="ACZPFK_07790"
     CDS             <1..292
                     /gene="pyrG"
                     /locus_tag="ACZPFK_07790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852166.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HIR1406250.1"
                     /translation="ADSGEFAENCPHKVIDFMPDQYGDIPKGGTMRLGAYPCTIAEGT
                     IMEKAYGTTSISERHRHRYEFNNEYRDALTQAGLELAGLSRQLAGRQFVRGQ"
     gene            423..2513
                     /gene="topA"
                     /locus_tag="ACZPFK_07795"
     CDS             423..2513
                     /gene="topA"
                     /locus_tag="ACZPFK_07795"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1406251.1"
                     /translation="MSKLVIVESPAKAKTIRKYLGDGYEVMASMGHIRDLPASQLGID
                     VERDYNPQYINIKGKEKLIKELKGAAKQSEGVLLATDPDREGEAISWHLAHLLGLDVE
                     AQDRVTFGEITKKGVTEGMANPRSIDLDLVNAQQARRVLDRLVGYKLSPFLWRKVRRG
                     LSAGRVQSVAVRLIVDRQKEIDAFIPEEYWNLDATLSAPGSAKKFTARLASTAAGEKL
                     TVTDKEQADRILAALEGKAYTVSKITKGTRKKVPAPPFITSTLQQEASRRLGFTATRT
                     MRAAQTLYEGVDIAGVGTTGLITYMRTDSLRLSDEAVAGAKAFISERWGDKYVNKAKR
                     TYKSKSATAAQDAHEAIRPSNPALTPDEVEKSISGDTAKLYRLIWSRFTASQMSDAIH
                     DTMNVEVAAGDYLFKASGYTVAFDGFTVLYEEATDEKEKKETALPPLEEGMALKLKNL
                     DAEQKFTQPPSLYTEATLIKALEENGIGRPSTYAPIITTIIDRGYVEREAKKLKPTLL
                     GLTVNELMMDEFSNIVDVKFSAQMEKNLDKVEAGKAEWVKTVDDFYTEFDKTLKKAEQ
                     DLEGKKIKVPDEETDEVCELCGRKMVIKTGRYGKFLACPGFPECKNTRRLVQAAPGEC
                     PLCGSRILVRKSKKGRVYYGCEKNPACGFMSWDEPTDEKCPECGKTLFKHGGKSGELV
                     CLAEGCGFRKPLKP"
     gene            2510..>3261
                     /gene="trmFO"
                     /locus_tag="ACZPFK_07800"
     CDS             2510..>3261
                     /gene="trmFO"
                     /locus_tag="ACZPFK_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR1406252.1"
                     /translation="MTPAVRVVGAGLAGCEAALLLADAGVPVTLYEQKPLHFSPAHKK
                     EGFAELVCSNSLKADRLDSASGLLKAEMRLLGSHLLDAAETARVAAGGALAVDRDVFS
                     EEVTRMVERHPGITVRRQEVTGLDFEDGGLAVVATGPLTEGALAEAIRQKTGRDSLSF
                     FDAAAPIVTAESIDRSKVFAASRYGRGSDDYLNCPFDKAGYEAFYAELVNAGRAELHD
                     ADRVNVYEGCMPVEIMARRGADTLRYGPLRPVG"
BASE COUNT          671 a         1026 c         1087 g          477 t
ORIGIN      
        1 cgccgattcc ggggagtttg cagaaaactg cccgcacaag gtcatcgact tcatgccgga
       61 tcagtacgga gatattccga agggcggcac tatgagactg ggggcttatc catgtacgat
      121 cgcagaagga actattatgg aaaaggctta cgggacgacg tccatttcgg agcgtcacag
      181 acatcggtat gagttcaaca acgaataccg ggatgccctg acccaggcgg ggctggagct
      241 ggccggcctg tcgcggcagc tggccgggcg gcagttcgtg cgcggccagt gagggcccgc
      301 cgaccggtgt gtaatccagc ggcgggcggc aaatgataaa gacgatcccg cgccgccggc
      361 gcgcggtaaa aagcgctttt tcggcggcgc tgacgatatc cgaaccgaca acgaggtgta
      421 atatgtctaa attggtcatt gtggagtccc ccgcaaaagc gaagacgatc cgcaaatatc
      481 tgggcgacgg ctatgaggtg atggcctcca tgggccacat ccgcgacctg ccggcaagcc
      541 agctcggcat cgacgtggag cgcgactata acccccagta catcaacatc aagggcaagg
      601 agaagctcat caaagagctc aagggcgcgg cgaagcagag cgagggcgtg ctgctggcca
      661 ccgaccccga ccgggagggc gaggccatca gctggcatct ggcccatctg ctggggctgg
      721 acgtggaggc gcaggaccgg gtgacctttg gcgagatcac caaaaaaggg gtcaccgagg
      781 gcatggccaa cccccgcagc atcgacctgg acctggtcaa cgcccagcag gcgcgccggg
      841 tgctggaccg cctggtgggc tacaagctgt cgccgttcct ctggcgcaag gtgcgccggg
      901 gcctctcggc gggccgggtg cagtcggtgg ccgtgcggct gatcgtcgac cgccaaaagg
      961 agatcgacgc ctttatcccc gaggagtact ggaacctgga cgccaccctc tcggcgcccg
     1021 gcagcgccaa aaagttcacc gcgcggctgg cctccaccgc ggcgggcgaa aagctcaccg
     1081 tcaccgacaa ggagcaggcc gaccgcatcc tggcggcgtt ggagggcaag gcctacacgg
     1141 tgtccaagat caccaagggc acccgcaaaa aggtgcctgc ccccccgttc atcacctcca
     1201 ccctgcagca ggaggccagc cgcaggctgg gcttcaccgc cacccgcacc atgcgggcgg
     1261 cccagacgct gtacgagggc gtggacatcg ccggggtggg caccaccggc ctgatcacct
     1321 acatgcgcac cgacagcctg cgcctgtccg acgaggcggt ggccggggcg aaagccttta
     1381 tcagcgagcg ctggggcgac aagtacgtca acaaggcaaa gcgcacctac aagagcaaaa
     1441 gcgccacggc ggcgcaggat gcccacgagg cgatccgccc ctccaacccg gccctcacgc
     1501 cggacgaggt cgaaaagagc atcagcggcg acacggccaa gctctaccgc ctgatctgga
     1561 gccgctttac cgccagccag atgtccgacg ccatccacga caccatgaat gtggaggtgg
     1621 ccgccgggga ctatctgttc aaggccagcg gctacaccgt ggcgttcgac ggcttcacgg
     1681 tgctttacga ggaggccacc gacgaaaagg aaaagaaaga gaccgccctg ccccctctgg
     1741 aagagggcat ggccctgaag ctgaaaaacc tcgacgccga gcagaaattc acccagccgc
     1801 cctccctcta caccgaggcg accctcatca aggcgctgga ggagaacggc atcggccgcc
     1861 cctccaccta cgcgcccatc atcaccacca tcatcgaccg cggctatgtg gagcgggagg
     1921 cgaaaaagct caaacccacc ctgctggggc tgacggtgaa cgagctgatg atggatgagt
     1981 tctccaacat cgtggacgtc aagttttcag cccagatgga aaagaacctg gacaaggtgg
     2041 aggcgggcaa ggcggaatgg gtcaagacgg tggatgattt ttacaccgaa tttgacaaga
     2101 ccctgaaaaa ggccgagcag gacctggagg gcaaaaagat caaggtgccg gacgaggaga
     2161 ccgacgaggt ctgcgagctc tgcggccgca agatggttat caagaccggc cgctacggca
     2221 agtttttggc ctgcccgggt ttccctgagt gcaaaaacac ccggcggctg gtgcaggccg
     2281 cgccgggaga gtgcccgctc tgcggcagcc gtatcctggt gcgcaagtcc aaaaagggac
     2341 gcgtctatta cggctgcgaa aagaaccccg cctgcgggtt catgagctgg gacgaaccca
     2401 ccgacgagaa gtgccccgag tgcggcaaaa ccctcttcaa gcacggcggc aaaagcggcg
     2461 agctggtctg tctggcagag ggctgcggct ttcgaaagcc gctcaagcct tgacgcccgc
     2521 ggtgcgggtg gtgggggcgg gcctggcggg gtgcgaggcg gcgctgctgc tggcggacgc
     2581 cggggtgccg gtcaccctct atgagcaaaa gcccctccac ttttcgcctg cacacaaaaa
     2641 agagggcttt gccgagctgg tctgctccaa ctcgctcaag gccgaccggc tggattcggc
     2701 cagcggcctg ctgaaagcgg agatgcgcct tttgggcagc catctgctgg acgccgccga
     2761 gacggcccgg gtggccgcgg gcggcgccct ggcggtggac cgggacgtct tcagcgagga
     2821 ggtcacccgc atggtggagc gccatccggg catcaccgtg cggcggcagg aggtcaccgg
     2881 tctggatttt gaggacggcg gccttgcagt cgtcgccacc ggcccgctca ccgagggggc
     2941 tctggccgag gccatccggc agaagaccgg ccgggacagc ctctcctttt tcgacgcggc
     3001 ggcccccatc gtcaccgccg agagcatcga ccggtcaaag gtctttgccg cgtcccgcta
     3061 cggccggggc agcgacgact atctgaactg tccgttcgac aaggcgggct atgaggcgtt
     3121 ttatgccgag ctggtaaacg ccgggcgcgc cgagctgcac gacgctgacc gggtgaacgt
     3181 ctacgagggc tgcatgccgg tggagatcat ggcccggcgc ggcgcggaca ccctgcgcta
     3241 tggcccgctg cggccggtgg g
//
LOCUS       DBMDYA010000289         3259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6641_length_3259_cov_1.483217, whole genome shotgun sequence.
ACCESSION   DBMDYA010000289 DBMDYA010000000
VERSION     DBMDYA010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_3259_cov_1.483217"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2726)
                     /locus_tag="ACZPFK_07805"
     CDS             complement(<1..2726)
                     /locus_tag="ACZPFK_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1406253.1"
                     /translation="MVTLKKTYRKNILRTIGGTMSRFLAIFAIVALGVGFLAGLLATT
                     PDMRYSGDKYFDDTHLFDIRTLGTLALSRGDVAAIRAVEGVGEVMPAYSADLLVNTPG
                     GDTLVTRIHSLPMAEIEEPEPQGWLNRFQVVEGRVPVKENECVIEAGSQYGGASVKIG
                     DLLTVSEENKDPDATLARREFKVVGVVQSSYYFSIEREPASVGSGTIGLIMYVGEDSF
                     ALDAYTDVYLTVQGAAELGCLGAEYEDLVDAVADRIEEISDARSAIRYREVKAEAEEK
                     LADARSEYEDAKAEADEKLADAEQQLADGRAEVERGEAKLKDAKAQISSGEKELAENR
                     ETLPGALTDKQQQLAAAQAALIDAKAQLEENEALLAEKQQQLADAKAQLETAKQLVST
                     LEPVLTQAEAQLPVLEAAIPPLQQTARQAQASYDAAVSSTDYRAKKEAYEQAEATLAA
                     SGGYASYEEWEAADPTAAQAAKAARDAAKAEFDAADAVVSPLKTSLDTANAQLAAAQR
                     AYETAKQSAEAARKQIDDAKEQIAVGEPQLAEGEKQLAEGQRQLADARKQVTEVEKQI
                     ASGQTALSLAPGLAQLQLDLAQYKLDSAKSEVAGGEAELSDAREQLEKGEAEYRTQKA
                     DAERQLADAEQQLADAQKQIDELEEPEWYVLTRDTNVSYASFKANVEKVEAVAKVFPI
                     FFFLVAALVVLTTMTRMVEEERLQIGTMKALGYSKGAIMMKYVWYALIASVAGSAAGL
                     LIGLNLLPVVIWNAYTMMYRLPRLYCLFNVPFAVFSSGTAIFCALAATLYACWTTLHE
                     CPASLMLPRAPKAGKRILLERVGFVWKRMKFTHKVTARNLFRYKKRFFMTVIGIPGCT
                     ALLVTGFGLHDSISDIVNRQFGEIFTYNLSITLKEEGDQKDDALQKLLNDGE"
     gene            complement(2720..>3259)
                     /locus_tag="ACZPFK_07810"
     CDS             complement(2720..>3259)
                     /locus_tag="ACZPFK_07810"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406254.1"
                     /translation="GILTPTQGRILLDGDLVSEYSQKQLTAYRRHDVGFVFQFYNLVQ
                     NLTALENVELASEICRDPLPADEMLRQVGLAQRMDNFPAQLSGGEQQRVSIARALAKN
                     PKILLCDEPTGALDYNTGKQVLALLQDTCRNTGRTVIVITHNSALTAMADRVIRIKSG
                     KALSNQVNAHPIPVEEIEW"
BASE COUNT          451 a         1097 c         1055 g          656 t
ORIGIN      
        1 ttttcgccgt cgttcagcag cttttgcagc gcgtcgtctt tctgatcccc ctcctccttg
       61 agcgtgatgg agaggttata ggtgaagatc tcgccgaact gccggttcac gatgtcgctg
      121 atggaatcgt gcagcccgaa gccggtcacc agcagcgccg tgcagcccgg gatgccgatg
      181 acggtcatga aaaagcgctt tttgtagcgg aacaggttgc gggccgtcac cttgtgggtg
      241 aacttcatcc gcttccagac aaagccgacc cgctccagca aaatgcgctt gcccgccttg
      301 ggcgcgcggg gcagcatcag gctggccggg cactcgtgca gggtggtcca gcaggcgtac
      361 agggtggccg ccagcgcgca gaagatggcc gtgccggagg agaacaccgc aaaggggacg
      421 ttgaacaggc aatagagccg gggcagccgg tacatcatgg tataggcgtt ccagatcacc
      481 acgggcagca ggttgaggcc gatcagcagc ccggccgcgc tgcccgccac cgaggcgatc
      541 agcgcgtacc agacatactt catcatgatg gcgcccttgc tgtagcccag cgctttcatg
      601 gtgccgatct gcagccgctc ctcctccacc atgcgggtca tggtggtcag caccaccagc
      661 gccgccacca ggaagaagaa gatgggaaag accttggcca ccgcctccac cttttccaca
      721 tttgccttga aagaggcgta gctcacattg gtgtcgcggg tgagcacgta ccactcgggc
      781 tcctccagct cgtcgatctg cttttgggcg tcggccagct gctgctcggc gtcggccagc
      841 tggcgctcgg cgtcggcttt ctgggtgcgg tattccgctt cgcctttctc cagctgctct
      901 ctggcgtcgc tcagctcggc ctcgccgccc gccacctccg attttgcgct gtccagcttg
      961 tactgtgcca ggtccagctg cagctgcgcc agcccggggg ccagcgagag ggccgtctgg
     1021 ccggaggcga tctgcttttc cacctcggtg acctgcttgc gggcgtcggc cagctgccgc
     1081 tgcccctcgg ccagctgctt ttcgccctcg gcaagctggg gctcgcccac ggcgatctgc
     1141 tctttggcgt cgtcgatctg tttgcgcgcc gcctcggcgg actgctttgc cgtctcgtag
     1201 gcgcgctggg cggcggccag ttgagcgttg gcggtgtcca gcgaggtttt cagcggcgac
     1261 accaccgcgt cggcggcgtc aaattccgcc ttggccgcgt cccgcgcggc ctttgccgcc
     1321 tgggcggcgg tggggtccgc cgcttcccat tcctcatagc tggcatagcc gccgctcgcg
     1381 gcaagggttg cctcggcctg ttcataggcc tctttttttg ccctgtagtc ggtggaggac
     1441 acagccgcgt cgtaggacgc ctgcgcctgc cgcgccgtct gctgcagcgg ggggatggca
     1501 gcctccagca ccggcagctg cgcctccgcc tgggtcagta ccggctcgag ggtcgagacc
     1561 agctgttttg cagtctccag ctgggccttg gcgtccgcca gctgctgctg cttttccgcc
     1621 agcagggcct cgttctcctc cagctgggct ttggcgtcga tcagcgccgc ctgggcggcg
     1681 gcgagctgct gctgcttgtc ggtcagggcg ccgggcaggg tctcccggtt ttcagccagc
     1741 tccttttcgc cgctgctgat ctgggccttg gcgtctttca gcttggcctc gccccgctcc
     1801 acctcggcgc gcccgtcggc cagctgctgt tcggcgtcgg ccaatttttc gtcggcctcg
     1861 gccttggcgt cctcatattc gctgcgggca tcggccagct tttcctccgc ctcggccttg
     1921 acctcgcggt agcggatggc gctgcgcgcg tcgctgatct cctcgatgcg gtccgccacg
     1981 gcgtcgacca ggtcctcgta ctccgcgccg agacagccca gctccgccgc gccctgcacg
     2041 gtcaggtaga cgtcggtgta ggcgtccagc gcaaagctgt cctcccccac gtacataatc
     2101 aggccgatgg tgccgctgcc cacgctggcg ggctcgcgct cgatggaaaa gtagtagctg
     2161 ctctgcacca cgcccaccac tttaaactcc cgccgggcca gcgtggcgtc cgggtccttg
     2221 ttctcctctg acaccgtcag caggtcgccg atcttcaccg aggcgccgcc gtactggctg
     2281 cccgcctcga tgacgcactc gttttccttg accggcaccc ggccctccac cacctgaaag
     2341 cggttcagcc agccctgggg ctccggctcc tcgatctcgg ccatgggcag gctgtggatg
     2401 cgggtgacca gcgtgtcgcc ccccggggtg ttgaccagca ggtcggcgga ataggcgggc
     2461 atcacctcgc ccaccccctc caccgcgcgg atggccgcca catcccccct gctcagggcc
     2521 agcgtgccca gtgtgcggat gtcgaacagg tgggtgtcgt caaagtattt gtcgccggaa
     2581 tagcgcatgt ccggcgtggt ggcgagcagc cccgcgagaa agccgacccc cagcgccacg
     2641 atggcgaaga tggccagaaa gcggctcatg gtgccgccga tagtgcgcag gatgtttttg
     2701 cgataggtct ttttcaaggt taccactcga tttcttccac agggatggga tgggcgttga
     2761 cctggttgga gagcgccttg ccgcttttga tgcggatgac ccggtcggcc atggccgtca
     2821 gggcgctgtt gtgggtgatg acgatgacgg tgcggccggt attgcggcag gtgtcctgca
     2881 gcagggccag cacctgcttg ccggtattgt agtccagcgc gccggtgggc tcgtcacaga
     2941 gcaggatctt tgggtttttg gccagcgccc gggcgatgga gacccgctgc tgctcgccgc
     3001 cggacagctg cgccggaaaa ttgtccatcc gctgcgccag gcccacctgc cgcagcatct
     3061 cgtcggcggg cagcgggtcg cggcagatct cgctggccag ctccacgttt tccagcgccg
     3121 tcaggttctg caccaggttg taaaactgaa agacgaaccc cacatcgtgg cggcggtagg
     3181 cggtgagctg cttctggctg tactcgctca ccaggtcgcc gtccagcagg atgcgcccct
     3241 gagtgggggt caggatgcc
//
LOCUS       DBMDYA010000290         3243 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6676_length_3243_cov_2.145767, whole genome shotgun sequence.
ACCESSION   DBMDYA010000290 DBMDYA010000000
VERSION     DBMDYA010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_3243_cov_2.145767"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="ACZPFK_07815"
     CDS             <1..580
                     /locus_tag="ACZPFK_07815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1406255.1"
                     /translation="ACHGAMPDTGVDAVVAGSALVMNLQTMVSREFSPVDPLVVTVGS
                     FHSGTRFNIVSGEAVLEGTIRSFSREVHEKLPAVVERIAKETAATFRAEAEVEYNSIT
                     EVLINDPAMTDLAFNAARKVADNALVMRTDRLQMGGEDFAEYTAHAKAAFCMLGGGGE
                     APQHSDHFVIDESAFEIGVALYAQVAVDALAE"
     gene            1031..1867
                     /locus_tag="ACZPFK_07820"
     CDS             1031..1867
                     /locus_tag="ACZPFK_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR1406256.1"
                     /translation="MNRILEKRELNEATVLLKLEAREIAAKALPGQFVIVRTGELGER
                     IPLTIEDYDREAGTIQIVFQKVGAGTLLLGALQEEDFVTDVAGPLGVATQLDGYKKVA
                     VVGGGVGCAIAYPQAKYLHAQGIPVDLIAGFRSREIVILEEEMRAACDRLLLATDDGS
                     YGQKGVVTNLLKEQIEAGAGYDRVIAIGPVPMMRAVCGVTKEYGIPTTVSLNPIMIDG
                     TGMCGGCRVTVGGKVRFACVDGPDFDGHEVDFEELMKRNGSYRDKEAEKKGHVCRLLG
                     VE"
     gene            1868..>3243
                     /gene="gltA"
                     /locus_tag="ACZPFK_07825"
     CDS             1868..>3243
                     /gene="gltA"
                     /locus_tag="ACZPFK_07825"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR1406257.1"
                     /translation="MPDLSMKKNAMPVQAPEKRRHNFEEVALGYTAELAAAEAERCLN
                     CKTKPCVSGCPVNVDIPDFIAKLRAGQLEAAFESISQHSSLPAVCGRVCPQETQCEKN
                     CVRGIKGEPVAIGRLERYVADRHNAGVQAAAKRPQPSGRRVAVIGAGPAGLTCAGELA
                     RRGHEVTVFESLHLAGGVLSYGIPEFRLPKSIVAREIEGLKALGVEIRTNEVIGKLHS
                     VDELFAAGYEAAFVSTGAGLPKFMGIPGEMLLGVYSANEYLTRINLMKAYRPEAETPL
                     ADVHKVAVVGGGNVAMDAARCALRMGAQEVTIVYRRSRAEMPARREEVEHAEEEGVVL
                     RTLTNPVEILADEAGHARALRCVRMELGEPDASGRRRPAPVAGSEFELEADAVIMAIG
                     TSPNPLVKNTTPDLETDRRGCFVVDESMHTSKQGVWAGGDAVTGAATVISAMGAGKKA
                     AAAISAYLA"
BASE COUNT          559 a          917 c         1274 g          493 t
ORIGIN      
        1 ggcctgccac ggcgccatgc cggacaccgg cgtggacgcg gtggtggcgg gcagcgccct
       61 ggtcatgaac ctgcaaacca tggtcagccg tgagttctcg ccggtggacc cgctggtggt
      121 cacggtcggc tcgttccatt ccggcacccg attcaacatc gtgtccggcg aggccgtgct
      181 ggagggcacc atccgcagtt tcagccgcga agtgcacgaa aagctgcccg cagtggtcga
      241 gcgcatcgcc aaggagacgg cggccacgtt ccgtgccgag gcggaggtgg aatacaacag
      301 catcaccgag gtgctcatca acgaccccgc catgaccgat ctcgctttca acgccgcgcg
      361 caaggtcgcc gacaacgccc tggtcatgcg caccgaccgg ctgcagatgg gcggggagga
      421 ctttgccgag tacaccgccc acgccaaggc cgcgttctgc atgctgggcg gcggtgggga
      481 agcgcctcag cacagcgacc attttgtgat cgacgagagc gcctttgaga tcggcgtcgc
      541 cctttatgcc caggtggcgg tggacgcgct ggccgagtaa ggcccgctgc agtgcagaca
      601 ggataaaacg gccgggccac ccggcaggcc cgccggcgcg ttcagccgcg ttcgagcaag
      661 ggggatcccc ttgttcgggc ccggcccttg cgccagcggg tttttgctgc aaatttttgg
      721 ggttgccccg cttgaggttg cgacggggca gggcattgat atataataat aaacagcggt
      781 ttgtcggatg cgggaaagcc tgcggcggga aagtggcccc caagcgccct ttcagcgcgg
      841 tgagacagcc ccccttgtgg agaggctcgg ccgccgcgcg gggcgggggc cgctgcgcag
      901 gcgctgtccg gtcggccgcc cacggggtgc agggcgaagc tcattttcaa aaagcgcatg
      961 ccctgcgccg tccgggcaaa aacgcagggg gccggggtcg gcttttctgc cagaggggag
     1021 aagaaagagc atgaacagga tactggagaa acgggaattg aacgaggcga cggtgctgct
     1081 gaagctggag gcgagggaga tcgcagccaa ggcgctgccg gggcagttcg tgatcgtgcg
     1141 cacgggggag ctgggcgagc gcatcccgct gaccattgag gactatgacc gcgaggcggg
     1201 gacgatccag atcgtgttcc agaaagtggg cgcgggcacg ctgctgctgg gggcgctgca
     1261 ggaggaggac ttcgtcaccg acgtggcggg gccgctgggg gtggcgaccc agctggacgg
     1321 gtacaaaaag gtggccgtgg tgggcggcgg cgtgggctgc gccatcgcct acccgcaggc
     1381 gaaatacctg catgcccagg ggataccggt ggacctgatc gcggggttcc gcagcaggga
     1441 gatcgtgatt ttggaagagg agatgcgggc ggcctgcgac cggctgctgc tggccaccga
     1501 cgatggcagc tacgggcaaa agggggttgt gaccaacctc ctgaaagagc agattgaggc
     1561 aggggccggg tacgaccggg tgatcgcgat cgggccggtg ccgatgatgc gggcggtgtg
     1621 cggggtgacg aaagagtacg gcatccccac cacggtgagc ctgaacccga tcatgatcga
     1681 cgggacgggg atgtgcggcg gctgccgtgt gacggtgggg ggcaaggtgc ggttcgcctg
     1741 cgtggacggg ccggacttcg acggtcacga ggtggacttt gaggagctga tgaagcgcaa
     1801 cgggagctac cgtgacaagg aagcggagaa aaagggccat gtgtgccgtc ttttgggggt
     1861 ggagtgaatg ccggatctgt cgatgaagaa gaacgccatg ccggtgcagg cgccggagaa
     1921 gcggcggcac aactttgaag aggtggcgct ggggtacacg gcggagctgg cggccgcgga
     1981 ggcggagcgg tgcctgaact gcaagaccaa gccctgcgtg tcgggctgcc ctgtgaacgt
     2041 ggatatccct gattttatcg ccaagctgcg ggcaggtcag ctggaggcgg cttttgagtc
     2101 gatctcgcag cactcctcgc tgcctgcggt gtgcgggcgg gtctgcccgc aggagaccca
     2161 gtgcgagaag aactgcgtgc ggggcatcaa gggggagccg gtggccatcg gccgtctgga
     2221 gcggtatgtg gcggaccggc acaacgccgg ggtgcaggcg gcggcgaagc ggccgcagcc
     2281 cagcgggcgc cgggtggcgg tgatcggcgc gggtccggcg gggctgacct gcgcggggga
     2341 gctggcccgg cgtgggcatg aggtgacggt gttcgagtcg ctgcacctgg cgggcggggt
     2401 gctgagctac ggcatcccgg agttccgtct gcccaagagc atcgtggcgc gggagatcga
     2461 gggtctgaaa gccctggggg tggagatccg caccaacgag gtgatcggca agctgcactc
     2521 ggtggatgag ctgtttgccg ccggttatga ggcggccttt gtctccacgg gtgcggggct
     2581 gccgaagttt atgggcatcc ccggggagat gctgctgggg gtgtattcgg caaacgagta
     2641 cctgacccgc atcaacctga tgaaagctta ccggccggag gccgagaccc cactggcgga
     2701 cgtacacaag gtcgcggtgg tgggcggggg caacgtggcg atggacgccg cgcgctgcgc
     2761 gctgcggatg ggcgcgcagg aggtcacgat cgtctaccgg cgcagcaggg cggagatgcc
     2821 cgcgcggcgg gaagaggtgg agcacgccga ggaggagggg gtcgttttgc gcaccctgac
     2881 caacccggtg gagatattgg cggacgaggc ggggcacgcg cgcgcgctgc gctgtgtgcg
     2941 catggagctg ggggagccgg acgcctcggg gcggcggcgg ccggcgccgg tggcgggctc
     3001 ggagtttgag ctggaggcgg atgcggtgat tatggccatc ggcaccagcc cgaacccgct
     3061 ggtcaaaaac acgacgccgg acctggagac cgaccggcgc ggctgctttg tggtggatga
     3121 gtcgatgcac acctccaagc agggcgtctg ggcgggcggc gacgcggtga ccggcgccgc
     3181 caccgtcatc tccgccatgg gcgcgggcaa aaaagccgcc gccgccatct ccgcgtatct
     3241 ggc
//
LOCUS       DBMDYA010000291         3241 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6679_length_3241_cov_1.548408, whole genome shotgun sequence.
ACCESSION   DBMDYA010000291 DBMDYA010000000
VERSION     DBMDYA010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6679_length_3241_cov_1.548408"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="ACZPFK_07830"
     CDS             complement(<1..247)
                     /locus_tag="ACZPFK_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406258.1"
                     /translation="MSGYLPPSTPAEQIFCRRVADAAGRCEATGRPQYLGFLDERQQV
                     LAAAQLSKIKWPEHLFFGGYPLAERKVLAVYGDEPPAE"
     gene            complement(248..721)
                     /locus_tag="ACZPFK_07835"
     CDS             complement(248..721)
                     /locus_tag="ACZPFK_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR1406259.1"
                     /translation="MGLFKKLGNLMGVPEEEYDDEYGDDIDFINNQSAYAGGEQEERY
                     EAPEAAPAAQQKRNKVVNINATTQLQVVLVKPERFDDASAIADHLNAKRTVVLNLEST
                     NKDISRRLVDFLSGVAYANNGQIKRVANSTFIITPYNVDIMGDLLDELESNGVFF"
     gene            complement(933..2192)
                     /locus_tag="ACZPFK_07840"
     CDS             complement(933..2192)
                     /locus_tag="ACZPFK_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR1406260.1"
                     /translation="MDKRRAVRWAVFLVLLIPVVYLAMQLVPLLFRGYETQTAVEATL
                     ADAVPARGVAVRSEHPLEQSGGVLGYLVEDGKRVAPGAAVGEVFAGEAQAQSSAKAGR
                     LGTELDMLEQSQSQGLASGTDVDQILKQQQQGLFDLLEVIDSGSYEGLVQAKSAVTLA
                     VNRLQAATGGAVDFSARIAALTQQRDAALAAAGAVEYLYAPSPGGYFSSMTDGLEQVL
                     TPQALDAMSASDLAALAENPGGEMAASSGKLIDSYEWYYYCLVDDAVADRFVDEDDAV
                     EVEIDFDYTGAKQIPATVISVDRDPSGGPAKVKLMCDYINAETVNLRAGTAQISFRRY
                     AGVRIEQSAVHIVTDDEGVQRRGVFVKYGNVVQFKRIDPTFENDQYVIVPAKIQLGSD
                     DESLKNEVLLYDEIITSGKDLYDGKLL"
     gene            complement(2185..2421)
                     /gene="hfq"
                     /locus_tag="ACZPFK_07845"
     CDS             complement(2185..2421)
                     /gene="hfq"
                     /locus_tag="ACZPFK_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR1406261.1"
                     /translation="MKATNLQDLFLNTVRRDRTPVTVYLLSGYQLRGTVRGFDNFVIV
                     LDCDGKQNMVYKHAVSTIIPARSVNLAADEGQNG"
     gene            complement(2482..>3241)
                     /gene="miaA"
                     /locus_tag="ACZPFK_07850"
     CDS             complement(2482..>3241)
                     /gene="miaA"
                     /locus_tag="ACZPFK_07850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1406262.1"
                     /translation="IDIRDPEELFSVAEFTALARQAVAEIAARGNLPILTGGTGLYIS
                     SLLEGLAFSDEPPDNGTRARLAREAAELGAAAMHTRLAGVDPETAAAIHPNNVKRVLR
                     ALELYEKTGRTMSGQRAASRPPEPPYDALVLGLDFADRETLYARIGLRIGRMLDEGLL
                     EEARRVYEHRQSWRTAAQAIGYKELFPFFEGEASLEGCADRLRQATRNYAKRQLTWFK
                     RMPGVCWLDAARPCLPPEAEAFLRAAGREASSKF"
BASE COUNT          543 a         1108 c          982 g          608 t
ORIGIN      
        1 cctccgccgg gggctcgtcc ccatacacag caagcacctt tcgctcggcg agaggatagc
       61 cgccgaaaaa aaggtgctcc ggccatttta ttttggacag ctgcgcggcg gcaagcacct
      121 gctggcgctc atccaaaaag cccagatatt ggggacggcc cgtcgcctcg cagcgcccgg
      181 ccgcgtccgc cacccggcga caaaaaatct gttccgccgg cgtgctgggg ggcagataac
      241 cgctcaatta aaagaacacc ccgttgctct cgagctcatc cagcaaatcg cccatgatgt
      301 ccacgttata gggcgtgatg atgaaagtgc tgttcgccac ccgcttgatc tggccgttgt
      361 tggcataggc cacgccggac aaaaagtcga cgagccggcg ggagatgtcc ttgttggtgg
      421 actcgaggtt gagcaccacg gtgcgcttgg cgttcaggtg atccgcgatg gcgctggcgt
      481 cgtcaaagcg ctccggcttg accagcacga cctgcagctg ggtggtggca ttgatgttga
      541 ccaccttgtt gcgcttttgc tgtgcggccg gggcggcctc cggcgcctcg tagcgctctt
      601 cctgctcgcc gcccgcgtag gcggactggt tgttgataaa gtcgatatcg tccccgtact
      661 cgtcgtcgta ttcctcttcg ggtacgccca tcaggtttcc aagtttttta aacagtccca
      721 tagctttgcc ctgcctttat catatttgtc ctgccgccgc cgcagcaatt atcctgtgcg
      781 gcgcttttca agcattgtta ctagtattat cacttttttt tgcggcaatg tcaacaaaag
      841 cctgaaaaat agttgcacag gcccgctttc cggccgcaga agccctttgc ccggcgcggg
      901 gaggaatttt ttccatcccc cgccctctgc gctcacagca gcttgccgtc gtataaatcc
      961 ttgcccgagg tgatgatctc atcgtacagc agcacctcgt ttttcaggct ctcgtcatcc
     1021 gacccgagct ggatctttgc cggcacaatg acgtactggt cgttttcaaa ggtggggtcg
     1081 atgcgcttga actgcaccac attgccgtac ttgacaaaaa ccccgcgccg ctgcacgccc
     1141 tcgtcatcgg tcacgatgtg caccgccgac tgctcgatgc gcacccccgc gtaccgccgg
     1201 aacgagatct gcgccgtgcc cgcccgcaga ttgaccgtct cggcgttgat gtaatcgcac
     1261 atcagcttga ccttggccgg gccgccggag gggtcgcggt cgacgctgat gaccgtcgcc
     1321 ggtatctgct ttgcgccggt gtagtcaaag tcgatctcca cctccacggc gtcgtcctcg
     1381 tccacaaagc gatccgccac cgcatcgtcc acaaggcagt aataatacca ctcatagctg
     1441 tcgatgagct tgccggatga ggccgccatc tcgccgccgg ggttctccgc cagcgcggcg
     1501 aggtccgatg cgctcatggc atccagcgcc tgcggcgtca acacctgctc cagcccgtcg
     1561 gtcatggagg aaaaatatcc ccccggcgag ggcgcgtaca ggtactccac cgcgccggcc
     1621 gccgccagcg ccgcgtcccg ctgctgggtg agggcggcga tgcgggccga aaaatccacc
     1681 gcgccgccgg tcgctgcctg cagccggttc acagccagcg tgacggcgct ctttgcctgg
     1741 acaagcccct catagctgcc gctgtcgatg acctccaaca gatcgaacag cccctgctgc
     1801 tgctgtttca agatctgatc cacatccgtg ccgctggcca gcccctgcga ctgggattgc
     1861 tccagcatgt ccagctcggt gccaagacga ccggccttgg cgctgctttg ggcctgcgcc
     1921 tcgccggcga agacctcccc caccgcggcg ccgggagcaa cgcgcttgcc gtcctccacc
     1981 agatagccga gcacgccgcc gctttgctcc agcggatgct cgctgcgcac cgccacgccc
     2041 cgggccggca cggcgtcggc cagggtggcc tccactgcgg tctgggtctc atagccgcgg
     2101 aacagcaacg gcaccagctg catggccaga tagacgaccg ggatcagcag gacgagaaag
     2161 acggcccagc ggacggcgcg ccgcttatcc attctgtccc tcgtccgcgg cgaggttcac
     2221 gctgcgggcc gggatgatgg tcgacacggc gtgcttgtag accatgttct gcttgccgtc
     2281 gcagtccagc acaatgacaa aattgtcaaa cccccgcacc gtgccgcgca gctgatagcc
     2341 gctgagcaga tagacggtca ccggcgtgcg gtcccgccgc accgtgttga gaaacaggtc
     2401 ctgcagattt gtggctttca tggcaatctt cctttcccac cgcgcgcgga gcgcggctgt
     2461 gggcggcaag ggccgccctt gttaaaattt tgatgaggcc tcgcggccgg ccgcgcgcaa
     2521 aaacgcttcc gcctcgggcg gcaggcaggg ccgggccgcg tccagccagc acacccccgg
     2581 catgcgctta aaccaggtca gctggcgctt ggcgtaattg cgggtcgcct gccgcagccg
     2641 gtccgcgcag ccctccaaag aggcctctcc ctcaaaaaag gggaaaagct ccttgtagcc
     2701 gatggcctgg gcggcggtgc gccagctctg ccggtgctca tacacccggc gcgcttcctc
     2761 cagcagcccc tcgtccagca tgcggccgat gcgcaggccg atgcgcgcgt aaagggtttc
     2821 cctgtccgca aagtccagcc ccagcaccag cgcgtcatag gggggctccg gcggccggga
     2881 ggccgcgcgc tggccggaca tggtgcggcc ggtcttctcg tacagctcca gcgcgcgcag
     2941 cacccgcttg acgttgttgg ggtgaatggc cgccgcggtc tcggggtcga caccggccag
     3001 acgggtgtgc atggcggcgg cgccgagctc ggccgcctcc cgcgccagcc gggcgcgggt
     3061 gccgttgtcc ggtggctcgt ctgaaaaggc cagcccctcg agcagcgagg agatgtaaag
     3121 ccccgtcccc cccgtcaaga tgggcaggtt gccccgcgcg gcgatctcag cgaccgcctg
     3181 ccgggccagt gcggtgaact ccgccacgct gaacagctct tccgggtcgc ggatgtcgat
     3241 c
//
LOCUS       DBMDYA010000292         3230 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6713_length_3230_cov_1.211250, whole genome shotgun sequence.
ACCESSION   DBMDYA010000292 DBMDYA010000000
VERSION     DBMDYA010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6713_length_3230_cov_1.211250"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            331..756
                     /locus_tag="ACZPFK_07855"
     CDS             331..756
                     /locus_tag="ACZPFK_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016535.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1406263.1"
                     /translation="MIYFLVFVGGLMLSAMQSINGLLSGYLGLFGTSLAVHVVGGVLL
                     VAFILLVLRQKIRLGPMPWYLYAAGIFGIALVTCNSFCIARLGAALTSCLSIAGQLIF
                     SILMDHFGLLGMRKVRFDKRRLPALALIAVGLVIVTAAG"
     gene            773..1246
                     /locus_tag="ACZPFK_07860"
     CDS             773..1246
                     /locus_tag="ACZPFK_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016535.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1406264.1"
                     /translation="MFYLLLVLGFVCGGINVVSKTINYQATNHLGTASGTLINYIVAS
                     LLSALVLLFTGPQQLAPSRFASVPWWLYLGGVCGLLAMLINVTSLQKISLFQSTTMLL
                     VGQLAGAVALDALLFHSMSLLKAAGVAVLAVGVVWDKRLCAAPEEAGAGGGKDGG"
     gene            complement(1706..3079)
                     /locus_tag="ACZPFK_07865"
     CDS             complement(1706..3079)
                     /locus_tag="ACZPFK_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406265.1"
                     /translation="MKWRELFSKKKEQEKSPEAAPQPKPEPPLVFGTLDFFPLESCED
                     VVVRGTVQGTVKKGDAVYVCNPGEDEGAIFLTTVTGIETAAGGTVERASGVKAALRLE
                     RGKKYLIKKGTVVFHRNASTAAVHQAYVSALGDAFVASQKLELSERDREGMSLTDCAE
                     VWRLFSWFRSTISETESQKQKSEENRDKLGALMCKKLLAAKEIYCVVNRKTGEPHLFS
                     HTIPQQGSYECTPPDICILTKAYKADLEKQYPEDRIEIRKIENGENGDGIKNFLADAF
                     YLNGACGVGILFEQASIGAEALVPKPDYSGVDPRKAPVTNPAVMRWMLLLNQMGQPET
                     ESERVVCGVYHRFLALALREARFLVPVRRSGPPPNPAQNESRTLQKGDCIEVFTGKGK
                     GERDAVRLYTDWKRLREGCGPEWNAVVQPIDELIGRMDCAINVTAHPESGYYLDEELY
                     RQIKAMQ"
BASE COUNT          514 a         1022 c          991 g          703 t
ORIGIN      
        1 cagcaggaaa aacagatagg agaactcgtc ctcctggcga cagaaagcct cccctttttc
       61 aaacacgcac agccgcagct ggggcagcgc ctgtggcggc aggatgctgc ggatgccgga
      121 gccgtcgatc caggcggccc gcgccgccac ctctttgatc tcgcgcaacg cgatcccctc
      181 tctccttttt tctttaacta tgacatatga catagttgat tgcaacccat atttgctaca
      241 ctgggggcga aaagaaaaaa gcgcgggccg ggcggcccgc ggacgccccg ggccatcccg
      301 ccggggcggc ggagaaaggg gaggctgcgg atgatctatt ttcttgtttt tgtcgggggc
      361 ctgatgctct cggccatgca gagcatcaac gggctgctca gcgggtacct ggggctgttc
      421 ggcaccagcc tggcggtgca tgtggtgggc ggggtgctgc tggtggcctt tattttgctg
      481 gtgctgcggc agaagatccg tctcggtccc atgccctggt atctgtacgc ggcgggcatt
      541 ttcggcatcg ccctggtgac ctgcaacagc ttctgcatcg cccgcctggg cgcggcgctc
      601 accagctgcc tgtccatcgc cgggcagctg atcttttcca tcctgatgga ccacttcggc
      661 ctgctgggca tgcgcaaagt gcgatttgac aagcgcaggc tgcccgccct ggccctgatc
      721 gccgtgggcc tggtcatcgt cacggccgcg ggctgaggga ggaggagaaa gaatgtttta
      781 tctgcttttg gtgttgggct ttgtctgcgg gggcatcaac gtggtgtcca agaccatcaa
      841 ctatcaggcg accaatcatc tgggcaccgc cagcggcacc cttatcaact atatcgtggc
      901 ctcgctgctc tcagcgctgg tgctgctgtt caccggcccc cagcagctgg cgccgtcccg
      961 ctttgcctcg gtgccctggt ggctgtacct gggcggcgtg tgcggcctgc tggccatgct
     1021 gatcaacgtc acctcgctgc agaagatcag cctgttccag tccaccacca tgctgctggt
     1081 gggccagctg gcgggggcgg tggccctgga cgcgctgctg ttccacagca tgtccctgct
     1141 caaggcggcc ggggtggcgg tgctggccgt gggcgtggtg tgggacaagc gcctgtgcgc
     1201 ggccccggag gaggccggcg ccgggggcgg gaaagacggc ggttgaaaga gggaccaaaa
     1261 aaagaatcaa gagacccgcg gggcggcaag tgaagggcgc gcgcggactg cccccccggc
     1321 cgccgacggc tttctctccg cctgcggacg actgtcccgg accgctgaaa gagagaaatt
     1381 ggaggcgcgg cccccccaaa gccgtgctgt gtgcggggcc atttttttca tccgggaaaa
     1441 agggcgcgcc cgaaaaggag aaagcggcgc ggggaggcgc ggagaggggg agccccccgc
     1501 ctgcttttca gcgcgtcggg ccgcggcagc ccaaacctcc ggtccgtttt gccgcggcgc
     1561 ccctcccgga tctttttatc aaaagaaaag cacagccgct ctcttttaag agagcggctg
     1621 tgctgcagat cggggtttgc ggtaaacggc gcgccgccgc ccggccgatc ccggagcggg
     1681 cttttctgcc gggcggctgt gccgctcact gcatcgcttt tatctgccgg tacagttctt
     1741 cgtccaggta gtatccgctt tccggatgcg ccgtcacatt gatcgcgcaa tccatccggc
     1801 cgatcagttc gtcgatcggc tgaaccacgg cgttccattc cgggccgcag ccctctcgca
     1861 ggcgcttcca atccgtgtac aagcgcaccg cgtcccgttc ccctttgcct tttccggtaa
     1921 agacctcgat gcaatcgccc ttttgcagcg tcctgctctc attctgggcg gggtttgggg
     1981 gagggccgct tctgcgcacg ggcaccaaaa acctggcctc cctcagcgcc agggccagaa
     2041 agcgatgata cacgccgcag accacccttt cgctctccgt ctcgggctgc cccatctgat
     2101 tgagcagaag catccagcgc atcacggcag ggttggtcac cggcgccttg cgcgggtcca
     2161 cgccgctgta atccggcttt ggcaccagcg cctcggcgcc gatggaggcc tgctcgaaaa
     2221 ggatgccgac gccgcaggcg ccgttcaggt agaatgcgtc ggccaaaaag tttttgatgc
     2281 cgtccccgtt ttcaccgttt tcgatttttc tgatctcgat ccggtcctcc ggatactgct
     2341 tttccaggtc ggccttatag gccttggtca ggatgcagat gtccggcggg gtgcactcat
     2401 agctcccctg ctgtggaatg gtgtgggaaa agagatgggg ctcgcccgtc tttctgttga
     2461 cgacgcagta gatctctttt gcggccaaga gctttttgca catcagcgcc cccagcttgt
     2521 cgcggttttc ctcgcttttt tgcttctggc tttccgtctc tgaaattgtg gagcggaacc
     2581 acgaaaagag acgccatacc tctgcgcaat cggtgaggct catgccctcc cggtcccgct
     2641 cgctcaactc cagcttctgc gacgccacaa atgcgtcccc cagcgcggag acataggcct
     2701 gatggaccgc cgccgtcgag gcgtttcgat gaaagacgac ggtgcccttt ttgatcagat
     2761 attttttgcc ccgttccagc cgcagcgccg ctttcacgcc gctggctcgt tccaccgttc
     2821 cgcccgccgc cgtttcgatg ccggtcaccg tggtgagaaa gatcgcgccc tcgtcctccc
     2881 cagggttgca gacgtagacg gcgtcccctt tttttacggt cccctgtacc gtcccgcgca
     2941 cgacgacgtc ctcacagctc tccagcggaa aaaagtccag cgtcccgaaa acgagcgggg
     3001 gctccggctt cggctgcggg gccgcctcag ggcttttttc ctgctccttt tttttagaaa
     3061 agagctctct ccatttcatc tgctgcctcc ttttgttctt tgtgatttgc ggcctttttg
     3121 gccgttttag atttggagcc gggataaaaa acttttttat gcccgttttg agagcgcggc
     3181 agggggcttt gtccgggccg acgccgttgt ggccgcgctc tcgcatcctg
//
LOCUS       DBMDYA010000293         3218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6735_length_3218_cov_1.456850, whole genome shotgun sequence.
ACCESSION   DBMDYA010000293 DBMDYA010000000
VERSION     DBMDYA010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_3218_cov_1.456850"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(442..1605)
                     /locus_tag="ACZPFK_07870"
     CDS             complement(442..1605)
                     /locus_tag="ACZPFK_07870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1406266.1"
                     /translation="MTAREMSEKVEQYVLDMRREFHMHPELSLKEERTRTRIAEELEK
                     MGIPYEIVEGHNVVGLVDSGKPGKSIAIRADFDALPLVEEVDVPYKSQTPGCMHACGH
                     DAHAACLLGIGKALLEMKDSFSGKVYLCFQAAEEAGRCADEIVAYLKSKGGVDNAVGM
                     HVAGGIEPGYVELREGARLSGAEIFYIDIKGVGGHGSRPDLTVDPIKAAVDVYQRLIT
                     IPTNHHSMFNTCVVSPCCLKAGERFNIVPETAHIEGTIRYYKMGDGEVVLEKIRQAAE
                     SICASHGCTVSVTNASGAKFPLINDAAAIAMAREIAVEIGLKLEDSLDPSAGSDNFAE
                     FLEAFPGFYMNLGTHSSRPGTSGNHHNPTFDVEESTFKKDVEFFLTYTQRFLA"
     gene            complement(1852..2097)
                     /locus_tag="ACZPFK_07875"
     CDS             complement(1852..2097)
                     /locus_tag="ACZPFK_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1406267.1"
                     /translation="MSEPTTIFSIYEDKDREIRQTLTEVYDALKEKGYNPINQLVGYI
                     LSEDPTYITTYGGARSKIRKIDRDDLLAALLKNFLGE"
     gene            complement(2229..>3218)
                     /locus_tag="ACZPFK_07880"
     CDS             complement(2229..>3218)
                     /locus_tag="ACZPFK_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR1406268.1"
                     /translation="IAEEICRYVGYNKVPSTIMKGVATARPSELQTFRSRMIEALVGY
                     GLYECETFSFYGKKAFDNINLPADDPLRRGVVISNPLGEDTSIMRTTAVPSLLEVVAR
                     NYNARAAEVAVFENATEYLPSDDPEKLPTENEKLILAVYGPGHDYLSLKGIVEQLLET
                     ARITGVTVAPNTEGTTYHPGRAAWLMLGDEKLAVIGELHPKVLANYGIKPRVCVADVS
                     LDLLFAHRGATPEFQPLPKHPAITRDLALVAAEDVPAAEIAARIEQGAGKLLESLSLF
                     DIYRGENIDAGKKSLAYNLVLRTADRTLTDEEADKAVAKVLKNLEQLGVVLRS"
BASE COUNT          597 a          931 c         1024 g          666 t
ORIGIN      
        1 ccctcttttt tggtggtcag gatctgcaga aagaccacaa agggctcttc caggctgccg
       61 taatagatct cgttcttaca gcggaccagg ggccgtccct tatatgtcaa aggcgcagta
      121 tatgccatgc ttgaatccct ccgtaaagat aaaaaataga ataaatttct taaatagtat
      181 accataaagc ggatggcaat acaacgggag cagcagaaag gacggtcgat ttctcagatc
      241 gctcattttc tcttgcgctt ctttcgcttc tttgcaccgg cggcttcaac cggacggcat
      301 gcgagagggg gaaagtgaag cctggagacg ccggcaggac agggatgtct gcccaaaaat
      361 ccgggccgag tcacagcgat gcctggatca aaaagggccc ccgggtgacc gggagccctt
      421 tgagaagacc ggaagccaag ctcaggcgag gaagcgctgg gtgtaggtga ggaagaactc
      481 tacatccttt ttgaacgtcg attcctccac gtcaaaggtg gggttgtggt ggttgccgga
      541 ggtgccgggg cggctggaat gggtgcccag gttcatgtag aagccgggga acgcctccag
      601 gaactcggcg aagttgtccg agccggcgct ggggtccagg ctgtcctcca gcttcaggcc
      661 gatttcgacc gcgatctcgc gcgccatggc gatggcggcc gcgtcgttga tcagcgggaa
      721 cttagcgccg gaggcgttgg tcaccgacac ggtgcagccg tgggaggcgc agatggattc
      781 ggcggcctgg cggatctttt ccagcacgac ctcgccgtcg cccatcttgt aatagcggat
      841 ggtgccctcg atgtgcgcgg tctcgggaac gatgttgaag cgctcgccgg ctttcaggca
      901 gcaggggctg accacacagg tgttgaacat gctgtgatgg ttggtgggga tggtgatcag
      961 gcgctgatag acgtccactg cggccttgat cgggtccacg gtcaggtcgg ggcgggaccc
     1021 gtggcccccc acgcccttga tgtcgatgta gaagatctcg gcgccggaca gccgggcgcc
     1081 ctcgcgcagc tccacatagc ccggctcgat gccgcccgcc acgtgcatgc cgacggcatt
     1141 gtccacgccg cccttggact tcaggtacgc cacgatctcg tcggcgcagc ggcccgcctc
     1201 ctccgcggcc tggaagcaga gatacacctt gccggaaaag ctgtctttca tctccagcag
     1261 cgccttgccg atgcccagca ggcaagccgc gtgggcgtcg tggccgcagg cgtgcatgca
     1321 gccgggcgtc tgggacttat agggcacgtc cacctcttcc accaggggca gagcgtcgaa
     1381 atcggcgcgg atggcgatgg atttgccggg cttgccgctg tcgacgaggc ccaccacgtt
     1441 gtggccctcg acgatctcat aggggatgcc catcttctcc agctcctcgg cgatgcgggt
     1501 gcgggtgcgc tcctccttga ggctcagctc tgggtgcatg tggaactcgc ggcgcatgtc
     1561 gaggacatac tgttccacct tttcggacat ttctcttgct gtcatattct ccactcctta
     1621 accaataggg atttacccct ttgaagcatt aaagctatta taaaaaattg ctcggatgat
     1681 gtcaataaaa acaagagcgc gcgggcaaat aaataccggg ccggcgatgg ccccaaagcg
     1741 gtgttgtgcg gcctcttgga tcgcccgccg cggggcagag gcagcgcggg acaaaacaaa
     1801 acacggcaca gcgaatgccg ctgtgccgtg tgctgcccga tcttaaaagg actactcgcc
     1861 gaggaagttc ttcagcaggg cggcaagcag atcgtcgcgg tcgatcttgc ggatcttgct
     1921 gcgcgcgccg ccgtaggtgg tgatataggt ggggtcctcg gaaagaatat atccgaccag
     1981 ctgattgatg gggttgtacc ccttttcttt cagcgcgtcg tagacctcgg tcagggtctg
     2041 cctgatctcg cggtccttgt cctcatagat cgaaaagatg gtcgtaggct cgctcatgtt
     2101 ggaaccccct ttgttgtttt tcattatacc caaaaaagga ggcggtttca agtgtcagtt
     2161 tgtgactgtt ttgccaaaaa tgttgagaca aaactgtgag ggaggaaaat atccccagtc
     2221 cgcagcgctc agctgcgcag caccacgccc agctgttcca gatttttcag caccttggca
     2281 acggccttgt ccgcctcctc gtcggtgaga gtgcggtcgg cggtgcgcag caccaggttg
     2341 taagcaaggc tctttttgcc agcgtcgatg ttctcaccgc ggtagatgtc aaacagggag
     2401 aggctctcca gcagcttgcc cgcgccctgc tcgatgcggg cggcgatctc cgccgcgggc
     2461 acgtcctctg cggccaccag cgccaggtcg cgggtgatgg ccgggtgctt gggcagcggc
     2521 tggaactccg gcgtggcgcc gcggtgggcg aacagcaggt cgagactgac gtccgccacg
     2581 cagacccgcg gcttgatgcc gtagttcgcc agcaccttgg ggtgcagctc accgatcacg
     2641 gccaattttt catcgcccag catcagccag gcggcgcggc cggggtgata ggtggtgccc
     2701 tcggtgttgg gcgccacggt cacgccggtg atgcgcgcgg tttccagcag ctgctccacg
     2761 atgcctttca ggctcaggta atcatggccc gggccgtaga cggccaggat cagcttttca
     2821 ttctcagtgg gcagcttttc cggatcatcg ctggggagat actcggtggc gttctcaaag
     2881 accgccacct cggcggcgcg ggcgttgtag ttgcgggcca ccacctccag caggctgggc
     2941 acggcggtgg tgcgcatgat gctggtgtcc tcgcccaggg ggttggagat gaccacgccc
     3001 cggcgcagtg gatcgtccgc gggcaggttg atgttgtcga acgccttttt gccgtaaaag
     3061 ctgaacgtct cgcactcgta caggccgtag ccgaccagcg cctcaatcat gcggctgcgg
     3121 aaagtctgca gctcgctggg ccgcgcggtg gcaacgcctt tcatgatggt gctgggcacc
     3181 ttgttgtagc ccacgtagcg gcagatctcc tcggcgat
//
LOCUS       DBMDYA010000294         3218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6736_length_3218_cov_1.335900, whole genome shotgun sequence.
ACCESSION   DBMDYA010000294 DBMDYA010000000
VERSION     DBMDYA010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_3218_cov_1.335900"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..851)
                     /locus_tag="ACZPFK_07885"
     CDS             complement(111..851)
                     /locus_tag="ACZPFK_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406269.1"
                     /translation="MSMNPNMANREVMDLILLDYQDKTPFLNLDFANVTTTDLAATRV
                     FAKGGRGAPNRVGFDGERTGTLKIDTQITPMKLYAMLSGSEVVKTASWMKRVVKTASN
                     NDSTFTITLDEEPAAGSVSVFEAGDDCGAKVECTVSGSTVTLTGGAAKEYIVYYFVKK
                     TAGVETVKFTSKSFPKAFIMHGTTPWKTEDDEIAMMHLVYYKAQPQANFSLAFSSTGD
                     PTTLSITCDLLANEDGDIYDMQLETGEN"
     gene            complement(1007..1642)
                     /locus_tag="ACZPFK_07890"
     CDS             complement(1007..1642)
                     /locus_tag="ACZPFK_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406270.1"
                     /translation="MPKIDRFDLISCHPVEVAGVGPMRSPTLREIARMGNAEYQACLS
                     LFLTTAKDLICASLHLVSGEDFDKLPQELRRQASVFRLLTASKKTRELLQRALSLFIA
                     GELQWQESVGFLVNPGPAAEGGQACEGVVNAENFDAVADLCLQFAAIESEKPGGKRFA
                     NEHSKKLYEKFLQKKEAYRRARSQKPNPDYQLGNLISALAAAHPSLNILNI"
     gene            complement(1648..2139)
                     /locus_tag="ACZPFK_07895"
     CDS             complement(1648..2139)
                     /locus_tag="ACZPFK_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406271.1"
                     /translation="MNLYELGRWKQQIAEKLAADPNIREILLPAPQPDTGSETPPSAN
                     PAPDLSEYLFNYEYVPGAQDEPKALISLEAAAPKVTGRGAFQVVLSVYALCHKSLLST
                     YSRAGVQGTRVDVLCADIDRLMSGLGGLGIGAAALTAVERYAPAPDYYGRVLTYSIPA
                     FNR"
BASE COUNT          625 a          932 c          972 g          689 t
ORIGIN      
        1 aaaagctttt gaatgtctta aagggctttg gcgtcgccgg ccgcccttat gccggggcgc
       61 cgcggggcgc cctcgcataa gggcggcctt tgccgcccct atgtaaccgg tcagttctcg
      121 ccggtctcca gctgcatgtc gtagatatcg ccgtcctcgt tggccagcag gtcgcaggtg
      181 atcgacaggg tggtcgggtc gccggtgctg ctgaacgcca gcgaaaagtt cgcctgcggc
      241 tgcgccttgt aatagacaag gtgcatcatg gcgatctcgt cgtcctcggt cttccagggg
      301 gtggtgccgt gcatgatgaa cgctttcggg aaagacttcg aggtgaactt cacggtctcg
      361 acgcccgccg tctttttcac gaaatagtag acgatgtact ccttggccgc gccgccggtg
      421 agggtcacgg tgctgcccga gacggtgcat tccacctttg cgccgcagtc gtcgcccgcc
      481 tcgaacaccg agacggaccc cgccgccggc tcttcatcca gggtgatggt aaaagtgctg
      541 tcattgttgg acgcggtctt gaccacccgc ttcatccagc tggcggtttt gaccacctcg
      601 ctgccggaca gcatcgcgta cagcttcatc ggggtgatct gggtgtcgat cttcagggtg
      661 ccggtgcgct cgccgtcaaa gccgacgcgg ttgggcgcgc cccggccgcc tttcgcaaaa
      721 acgcgggtgg cggcaaggtc ggtggtggtg acgttggcga agtccaggtt caaaaacggg
      781 gtcttgtcct ggtagtccag caggataagg tccatgacct cgcggttcgc catgttggga
      841 ttcatactca tatctaacct ccaaaaattt atttatcgag tgatctgaac caggccgccg
      901 ggtcggattc tccgccccag gcggcatagt tcatctcacg gataacgatt tgtcgcatat
      961 aggtctgacg ggtgatctgg tcgtgaagct gatagaccgt caggtcctat atgttcagga
     1021 tgttcaagct gggatgtgcc gcggccaggg ccgagatcag gttgcccagc tgatagtccg
     1081 ggtttggttt ttgcgagcgc gcccgccggt aagcctcttt tttttgaaga aacttttcat
     1141 aaagcttttt cgagtgctcg ttggcaaagc gcttgccgcc gggtttttcg ctttcgatcg
     1201 ccgcaaattg caggcagaga tcggccacgg cgtcaaagtt ttccgcattg accacgccct
     1261 cgcaggcctg tccgccctcg gcggcggggc cggggttcac caaaaagccc acgctttctt
     1321 gccattgcag ctccccggcg ataaaaaggg agagcgcccg ctgcaaaagc tcgcgcgttt
     1381 ttttcgacgc ggtcagcagg cgaaagacgg aggcctgccg gcgcagctcc tgcggcagct
     1441 tgtcgaagtc ctcaccggac acaagatgca gcgaggcgca gatcaggtcc tttgccgtgg
     1501 tcaaaaacag cgacaggcag gcctggtatt cggcgttgcc catccgggcg atctcccgca
     1561 gggtggggga gcgcatcggg ccgacgcccg ccacctccac cgggtggcag ctgatcaggt
     1621 cgaagcgatc aatcttcggc atggccctca cctgttgaac gcggggatgg aataggtgag
     1681 cacgcggccg tagtagtccg gcgcgggcgc atagcgctcg acggcggtga gggccgcagc
     1741 gccgatgcca agccccccga ggccgctcat cagccggtcg atgtcggcgc agagcacgtc
     1801 cacacgggtg ccctgcacac cggcccggct gtaggtggaa agcaggcttt tgtggcagag
     1861 cgcgtacacg gagagcacta cctgaaaggc gccgcggccg gtcaccttgg gcgccgcggc
     1921 ctccaggctg atcagggcct ttggctcgtc ctgcgcgccg gggacgtatt cgtaattgaa
     1981 gagatattcc gacaaatcgg gcgccgggtt tgcggagggc ggcgtttcgc tcccggtatc
     2041 cggctgtggt gcgggcaaaa ggatctcccg tatgttcgga tcggccgcca gcttttccgc
     2101 gatctgctgt ttccagcggc cgagctcgta taggttcatg acattacctc caattccatc
     2161 gttgcggccc gcccacgacg ggcctcaagc agctgtggtc ctcggccttt gcaagcgcgg
     2221 ggccgactct gtgcgcccgc ccagtgggct tgccggtgcg gccgactttc ccgtcccgcc
     2281 aggctctgca aagcgcgcag ggccgcacgg caaaacggtt ttggagcctt aaaaggcagg
     2341 cggcgaacaa agacaaaaac aagaagaatt gtaaaaagct gtaaacgggc ggctttcagg
     2401 ggggcgcaca aggccccctt tttttattgc cgggccgagg ggcacagcct gataggccaa
     2461 gctctccccg gggccgtccc gggcccggcg cagatgattt ttctttaaaa gccgcggctc
     2521 ctccccgcgg ttcataacaa attcctctcg ctattcgccg cggcgcagcc ggtacagcgt
     2581 gttatatttt tcttccagaa aaacgcgtct gcgatttcta agcggagggc cgtcggcggt
     2641 attttttgat ttttgatatt cggtacacgc ggggcggacc ccggcccggt ggatgcccgc
     2701 gcaggtgtgc gagaacttgc aggtgctgca gatataaagc cggtagaact cgtcctcgag
     2761 gccgcggtcc cggtaccact gccgcgtctt ctctataaat gaggtcgttt tggagccctc
     2821 ctttcgcatt ttttcctgcc cagagcggca gtccctatgg accttttact gtgtgaatga
     2881 atttcataaa aatattactt ttacaaattc aataaacaaa aaataaaagt tcccatgtct
     2941 cgggaaaaac aaattttggg gtgaaaagat cgtccctgta tccccattat atcctaagaa
     3001 tgaaacaagt caatataaat tttacttttt aaaattcatt aagaatacaa aacagcgaaa
     3061 aaagcgcatt ccgcgctgaa aaaagccgca caagagcggg ccgagaggtc aggcctgcag
     3121 ctcggacatc aggcggtcgg cggtcttcca gatatgggtg cgccccgtca gccagtcaat
     3181 cgaaacgccc atgatgtccg ccagcgagat gagcgcgt
//
LOCUS       DBMDYA010000295         3214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6749_length_3214_cov_1.557340, whole genome shotgun sequence.
ACCESSION   DBMDYA010000295 DBMDYA010000000
VERSION     DBMDYA010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6749_length_3214_cov_1.557340"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="ACZPFK_07900"
     CDS             complement(<1..1429)
                     /locus_tag="ACZPFK_07900"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1406272.1"
                     /translation="MKKKLLSLLVAVTMIFSCLPFASLSAAAAEVDGNLKRTVQTAQQ
                     VVKGFNACTTDAERRTYLGISNNNISNDSFRAVYGRLTGDAALQLQDAGLLESEEVQT
                     ALQTARATALYLQPYFLKDENGKRTFVLYASLTSSISKRDWNAYLLYLPAGQDGLSGS
                     WYVTNKAHRKAVNVANFNGKSFADVQNKEAQNAEHWILIADQSAAREPKVADPYEAIR
                     FLAGQLATEGSVAEGYFSTRGQGASLDGRGKNFAPDINAALAAAGVTDEDSTYAWRIY
                     KCKESVGEHGYNIFWTNSNLDDGGRFPCLKYNTATSEWVEGTATARQTTVEGITFLII
                     DGGSFVPNETQPDTAVKYTLSYDANGGDEKTLPASVQAEEYEVLPVDFSVIPMREGYR
                     FLGWDTIPDPTSPKYHQGFMGAAELMMPDHDLTLFAIWAAEATPTPTATPTPTPTPTA
                     TPTPKPTATPTPKPTATPTPKPTATP"
     gene            complement(1708..>3214)
                     /locus_tag="ACZPFK_07905"
     CDS             complement(1708..>3214)
                     /locus_tag="ACZPFK_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1406273.1"
                     /translation="GSLYQQTYNGVGYGVTGYKVYRTQKELVEGDLTEAQLQANLAQA
                     QEAYDQAVKNYEAAVAAAGVVFDADGNIESSAAQTDKEIAEKDVETLTQEREALGERP
                     ADTAELEALKDAVAKAEAAYNEAKAKYDTAKAEYDAYQALADEKAANDARLAELPGLI
                     AAEEGKKVDEAALQAQVQSAEQAYQDALTAQTNLPDTATDEDRSAAQAAVDTAAAAKA
                     AADQALTDAQSANAAAAEAIAALESEKTALEARNAELQAQMADPAPAEPSDAKMKEAE
                     LAKLKAEAAFQADPTYQAQLAWDQADVQLNTKTADAVARVNAAQLLLDQCSTALIARD
                     EAETALEVAKAAVGGDKAARAKVAVLLSAGFKAAEEEVPVTNGAFVYEEGYSYRVAIS
                     YYAESRAYSPVVNPPVVVIPDDRVPVGPGPSGGGTPVTIIDDDVPMGALPGKSRHRRS
                     LYTILDDDVPLGALPKTSGSRGAAVGGLGAFLLAAGAALGLTKKRKKEDEE"
BASE COUNT          486 a         1027 c          985 g          716 t
ORIGIN      
        1 tcggggtcgc cgtcggcttc ggtgtcgggg ttgccgtcgg cttcggggtc ggggtcgccg
       61 tcggcttggg cgtcggggtc gccgtcggcg tcggcgtcgg cgtcggcgtt gccgtcgggg
      121 tcggggtggc ctccgccgcc cagatggcaa aaagggtcag gtcatggtcg ggcatcatca
      181 gctccgcagc gcccatgaac ccctggtgat atttggggct ggtggggtcg gggatggtgt
      241 cccagccgag gaagcggtac ccctcgcgca tggggatgac gctgaaatcc accggcagga
      301 cctcatactc ctccgcctgc acggaggcgg gcaaggtttt ctcgtcgccg ccgttggcat
      361 cgtaagagag ggtatacttc accgccgtat cgggctgggt ctcgttgggc acaaaggagc
      421 cgccgtcgat gatgagaaaa gtaattccct ccaccgtggt ctgccgggcc gtcgcggtgc
      481 cctcgaccca ctcgctggtc gcggtgttgt atttcaggca ggggaacctg ccgccgtcgt
      541 ccaggtttga attggtccaa aaaatgttgt agccgtgctc gcccacactc tctttacatt
      601 tataaatgcg ccaggcgtag gtggaatcct cgtctgtgac cccggcggcc gcgagagcgg
      661 cattgatgtc cggcgcaaag ttcttgcccc gcccatccag cgaggcgccc tggccgcggg
      721 tgctgaaata accctcggcc acactgccct cggtcgccag ctgcccggcc aaaaagcgga
      781 tggcctcata gggatcagcc actttcggct cgcgcgcggc actctgatcg gcgatcagga
      841 tccagtgctc cgcgttctgt gcttccttat tctgcacgtc cgcgaacgac ttgccgttga
      901 agttcgccac attcacggct ttgcggtggg ccttattggt gacataccag ctgccggaca
      961 ggccgtcctg ccccgcgggg agatagagca gataggcgtt ccagtcccgt ttggaaatgc
     1021 tgctggtcag cgaggcataa agcacaaagg tgcgcttgcc gttttcatcc tttaaaaagt
     1081 agggctgcag atacagggca gtcgcccggg cggtctgcag ggcggtctgc acctcttcgc
     1141 tctccaacag cccggcatcc tgcagctgca gcgcggcgtc gcccgtcagg cgcccgtata
     1201 ccgcgcgaaa gctgtcgttg gagatattgt tgttgctgat gcccaggtaa gtacggcgct
     1261 cggcgtcggt ggtgcaggcg ttgaagcctt tcacgacctg ctgggccgtc tgcaccgtcc
     1321 gtttcaagtt gccgtcgacc tccgccgcgg ccgcgctcag gctggcaaac ggcaggcagg
     1381 aaaaaatcat tgtcacggcc accagcaggc tgagcaattt ctttttcata gttttttctc
     1441 ctgttcttga cgcgtctcac ccgcgttttt tctatgggta cgtcccttta aaaagcgacg
     1501 ctgctttccg attcttatta tcgcacaaac gggtcaccag ttggtaacaa aatcgtgcca
     1561 aattttttta tttttttgac tattattact ttttggtgac cttggacttt tgccgggatt
     1621 tcccgcgcca aaaaagcaaa aaagaaagac acccgagggt gtctttcttt tttcatgctt
     1681 tgggatggag ggctgcggtg ttaccgctta ctcctcatcc tcctttttgc gctttttggt
     1741 caggccgagg gccgcgcccg cagccagcag gaaagcgccc aacccgccca cggccgcgcc
     1801 gcgggaacca ctggtcttgg gcagcgcgcc cagaggcacg tcgtcgtcca ggatggtgta
     1861 caggcttctt ctgtgacggc tcttgccggg cagagcgccc atgggcacat cgtcgtcaat
     1921 aatggtaacg ggggtgccgc cgccgctcgg tcccgggcca acgggcaccc ggtcatccgg
     1981 aatcacgacc accggaggat tgacaaccgg gctatacgct ctggactcgg catagtagct
     2041 gatcgctaca cggtagctgt agccctcctc gtagacaaag gcaccgttgg tcacaggaac
     2101 ctcttcttcc gccgccttga agccggcgct cagcagaacg gcaaccttgg cgcgggccgc
     2161 cttgtcgccg cccaccgccg ccttggccac ctcgagggcg gtctcggcct cgtcgcgggc
     2221 aataagggcg gtgctgcact gatccagcag cagctgggcc gcatttacac gggccaccgc
     2281 atccgcggtc ttggtattga gctgcacatc cgcctgatcc catgccaatt gagcctggta
     2341 ggtcgggtca gcctggaaag cagcctccgc tttcagcttc gcgagctctg cctctttcat
     2401 ttttgcatcg gacggctccg ccggggcggg gtcggccatc tgcgcctgca gttctgcatt
     2461 gcgcgcttcc aaagccgtct tttcgctctc gagcgccgcg atcgcctctg cggcggccgc
     2521 attcgcgctc tgcgcgtccg tcagcgcctg atcggccgcg gccttggccg ccgcagcggt
     2581 atccaccgcc gcctgcgccg cgctccgatc ctcatcggtc gcggtatcgg gcagattggt
     2641 ctgcgcggtc agggcgtcct gataggcctg ctctgcgctc tgtacctggg cctgcagggc
     2701 ggcctcatcg acctttttgc cctcctcggc cgcgatcagg ccgggcagct ccgccaaacg
     2761 ggcgtcgttg gccgctttct cgtctgccag cgcctggtat gcgtcgtact ccgccttggc
     2821 cgtgtcgtac tttgccttgg cctcgttgta cgccgcctcc gctttcgcca cagcgtcttt
     2881 caacgcttcc agctccgcgg tatcggccgg gcgctcgccc agggcctcgc gctcttgggt
     2941 cagggtttcg acatcctttt cggcgatctc cttgtcggtc tgcgccgcgc tggattcgat
     3001 gttcccgtcg gcgtcaaaga cgacgccggc ggcggccacg gcggcctcat agttcttgac
     3061 cgcctgatcg taagcttcct gcgcctgggc caggttggcc tgcagctgcg cttcggtgag
     3121 gtcgccctca acgagttctt tctgggtgcg atagaccttg tagccggtca caccgtagcc
     3181 gacgccgtta taggtctgct ggtaaaggct gccc
//
LOCUS       DBMDYA010000296         3213 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6752_length_3213_cov_1.624357, whole genome shotgun sequence.
ACCESSION   DBMDYA010000296 DBMDYA010000000
VERSION     DBMDYA010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6752_length_3213_cov_1.624357"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="ACZPFK_07910"
     CDS             <1..288
                     /locus_tag="ACZPFK_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024687.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1406274.1"
                     /translation="DESSPRWVNAYWADGVRESFKSTLEIIAVDRPSLIADVSAMIAN
                     MKVPIYAFTARQNADGRSSILVTVGINNIDHLGSVITKLKKVRDVVSVTRT"
     gene            339..797
                     /gene="dtd"
                     /locus_tag="ACZPFK_07915"
     CDS             339..797
                     /gene="dtd"
                     /locus_tag="ACZPFK_07915"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1406275.1"
                     /translation="MRAVVQRVRSAQVTIDGQETRAIGRGLLILLGVAAEDTMEDARL
                     LARKCAELRVFEDGQGKLNLSAPELGYEAMVVSNFTLQADTKKGRRPSFIRAAKPPLS
                     VEAYHLFVECVRGYGLARVETGEFGADMQIALVNDGPVTLVLDTEEWKRG"
     gene            846..1418
                     /locus_tag="ACZPFK_07920"
     CDS             846..1418
                     /locus_tag="ACZPFK_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1406276.1"
                     /translation="MKFYHLNAPGPYYTNSFLLISEAGHGVVIDACAAPEKYLALLEQ
                     EHASLAAILQTHGHFDHVGSIPALKRATGARVYLNKNDAALFSLAADESLEDGQVVQI
                     DELAFQVITTPGHTPGSVCLQLGERLFSGDTLFAGDIGRTDLEGGSYTEIQKSLHKLL
                     SRVQGDPQVLPGHEEFSTFSAEKAYNRYLR"
     gene            1442..2938
                     /gene="hemZ"
                     /locus_tag="ACZPFK_07925"
     CDS             1442..2938
                     /gene="hemZ"
                     /locus_tag="ACZPFK_07925"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR1406277.1"
                     /translation="MLLSVSGLPVYEIEHIARMFYRDLLVREGRARPLPGDVLEPASR
                     HRAAALCDGLIAVRAGSRRILAALRTSQGTFVKMGTRPGEEELEFALCRLLYELLCQR
                     TGTRPPWGVLTGVRPVRLVHELWRQGADDEAVKTYFTGRCLVSPQKARLALATAHAQQ
                     PVVSRLPKRSCSLYIGIPFCPSRCSYCSFVSRTIGREQALVQPYLDKLCLEVERTVEI
                     IRELGLRLCTVYIGGGTPTAITADQLRQLMGTVRRCVDLSSLEEYTVEAGRPDCTDEE
                     KLSVLLEYGATRISINPQTFSDEVLAAIGRRHTAQAILDCYAAARRVGHKNINMDLIA
                     GLPRDTVEGFERSLRQTIDLGPENITVHTLTLKRASNLVIEHARDEYEDVAAMVEKNR
                     LLLGAGYAPYYLYRQKGTLQNLENTGFTKPGFAGLYNIYIMEELHTIFSTGAGGSTKL
                     KAPGGTRIERIFNYKYPAEYIAGFDTILERKKRIGEFYGRYLDPEASC"
     gene            2904..>3213
                     /locus_tag="ACZPFK_07930"
     CDS             2904..>3213
                     /locus_tag="ACZPFK_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012662.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR1406278.1"
                     /translation="MAAIWIPKRLVEIGLVNTAAENPESFISHCEIEYQSRVEAAARE
                     VLAQRRSIVMLTGPSASGKTTTAHKLADCIRKNGSYSCVISLDNFFKNLEDYPRLPDG
                     S"
BASE COUNT          621 a         1024 c         1035 g          533 t
ORIGIN      
        1 gatgagtcga gcccccgctg ggtgaacgcc tactgggccg acggcgtgcg ggagagcttc
       61 aagtccacgc tggagatcat cgccgtcgac cggccctcgc tgatcgcgga tgtcagcgcc
      121 atgatcgcca acatgaaagt gcccatctac gctttcacgg cccggcagaa cgccgacggc
      181 cgcagcagca ttttggtcac ggtgggcatc aacaacatcg accatttggg ctcggtcatc
      241 accaagctga aaaaggtcag ggacgtggtc tcggtcaccc gcacctgagc gggccgagac
      301 agcggaacaa gcggaagaaa gagaaaaaag gagcagccat gagagcagtt gtacagcggg
      361 tgcgttccgc gcaggtgacc atcgacgggc aggagacccg cgccatcggg cgcgggctgc
      421 tgatcctgtt gggggtggcc gcagaggata ccatggagga cgcgcggctg ctggcccgca
      481 aatgcgccga gctgcgcgtg tttgaggatg gacagggcaa gctgaacctg tcggcccccg
      541 agctgggcta tgaggccatg gtggtcagca atttcaccct gcaggcagac acgaaaaaag
      601 ggcggcggcc cagctttatc cgggcggcga agccgcccct ttcggtggag gcctaccacc
      661 tgtttgtgga atgcgtgcgc ggctacggcc tggcgcgggt cgagacgggg gagttcggcg
      721 cggatatgca gatcgccctg gtgaatgacg gcccggtgac gctggtgctg gacactgagg
      781 agtggaagcg gggctgatcc ccggcgcctc tgccgcgcaa aagtttaaag aaaaaggtga
      841 tcttcatgaa attttaccat ctcaacgcgc ccggccccta ttacaccaac agctttttgc
      901 tcatcagcga ggccgggcac ggcgtggtga tcgacgcctg cgccgcgccg gaaaaatatc
      961 tcgccctgct ggaacaggag cacgcgtccc tggccgccat cctgcagacc cacggccatt
     1021 ttgaccatgt gggctcgatc ccggcgctga aaagggccac gggcgcacgg gtctacctca
     1081 acaaaaacga cgccgcgctg ttcagcctcg cggcggacga gagcctggag gacgggcagg
     1141 tggtacagat cgacgagctg gcgttccagg tcatcaccac cccggggcac acccccggca
     1201 gcgtctgcct gcagctgggg gagcgcctgt tctccggcga tacgctcttt gcgggggaca
     1261 tcggccgcac cgacctcgag ggcggcagct acaccgagat ccaaaaatcg ctgcacaagc
     1321 tgctgagccg ggttcagggc gacccgcagg tgctgcccgg ccacgaggag ttctccacct
     1381 tttcggcgga gaaagcctat aaccgctatc tgcgctgaga gagggaaccc acaccgtgtc
     1441 gatgctttta tccgtcagcg ggctgccggt ctatgagatc gagcacatcg cccgcatgtt
     1501 ctaccgcgac ctgttggtgc gcgagggccg cgcccggccc ctgcccggcg atgtgctgga
     1561 gcccgcgtcc cgccaccggg cggcggcgct ctgcgacggc ctgatcgcgg tgcgggcggg
     1621 cagccgccgc atcctggccg ccctgcgcac ctctcagggc acttttgtca aaatgggaac
     1681 ccgccccggg gaagaagagc tggaattcgc cctctgcagg ctgctctacg agctgctctg
     1741 ccagcgcacc gggacccggc ccccttgggg cgtgctcacc ggtgtgcggc cggtgcggct
     1801 ggtgcacgag ctgtggcggc agggggcgga cgatgaggcg gtgaaaacct atttcaccgg
     1861 ccgctgcctg gtctcgccgc aaaaggcccg cctcgccctg gccacagccc acgcccagca
     1921 gccggtcgtc agccgtctgc caaagcgcag ctgcagcctt tatatcggca tccctttctg
     1981 cccctcccgg tgcagctatt gctcctttgt ctcccgcacc atcgggcggg agcaggcgct
     2041 ggtgcagccc tatctcgaca agctctgcct ggaggtggag cgcacggtgg agatcatccg
     2101 cgagctgggg ctgcggcttt gcacggtcta tatcggcggg ggcaccccca cggcgatcac
     2161 ggcggaccag ctgcgccagc tgatgggcac ggtgcgccgc tgcgtcgatc tctcctccct
     2221 ggaggagtac accgtcgagg cggggcggcc cgactgcacc gatgaggaaa agctttccgt
     2281 cctgctggaa tacggcgcca cccgcatctc catcaacccg cagaccttca gcgacgaggt
     2341 gctcgccgcc atcgggcgca ggcacaccgc gcaggccatt ctggattgct acgccgccgc
     2401 gcggcgggtg ggtcacaaaa acatcaacat ggatctgatc gccgggctgc cccgggacac
     2461 ggtggagggc tttgagcgca gtttgcgcca gaccatcgac ctgggaccgg aaaacatcac
     2521 cgtgcatacc ctgaccttga agcgtgcctc caatctggtc atcgagcacg cccgggacga
     2581 gtatgaggac gtggccgcca tggtggagaa aaaccggctg ctgctcgggg ccgggtacgc
     2641 cccctattat ctctaccgcc agaaagggac gctgcagaac ctggagaata cgggcttcac
     2701 caagccgggc tttgccgggc tgtacaatat ctatatcatg gaagagctgc acaccatctt
     2761 ctccaccggc gcgggcggct ccaccaagct gaaagcgccc ggaggcaccc gcatcgagcg
     2821 catcttcaat tataaatacc cggcggaata tatcgccggc tttgatacga tactggagag
     2881 aaagaaaagg ataggtgaat tctatggccg ctatctggat cccgaagcgt cttgttgaga
     2941 tcggcctggt gaacacggcg gctgaaaacc ccgaaagctt catcagccat tgtgagatcg
     3001 agtatcagag cagggtggag gcggccgcgc gggaggtgct ggctcagcgg cgcagcatcg
     3061 tcatgctcac cgggccctcg gcgtccggca aaaccaccac agcccataag ctggccgact
     3121 gcatccgcaa aaacggaagc tattcctgcg tgatctcgct ggacaacttt ttcaaaaacc
     3181 tggaggatta cccccgcctg ccggacggca gca
//
LOCUS       DBMDYA010000297         3200 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6780_length_3200_cov_1.188448, whole genome shotgun sequence.
ACCESSION   DBMDYA010000297 DBMDYA010000000
VERSION     DBMDYA010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_3200_cov_1.188448"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="ACZPFK_07935"
     CDS             <1..463
                     /locus_tag="ACZPFK_07935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1406279.1"
                     /translation="YVNGWERYMQHTHTAFFNEHSLELFEEGGSEEALLHRLFDMGVQ
                     IVVETLGKEGCAVVTRQERFAVPAYDVPVVDTTGAGDTFNSSFVFGLSRGWALGESAR
                     FATAAANMAVMAQGPRSGITSEEKVRAFMRQNAHLLGGKTPHIDTKEVGSK"
     gene            460..1482
                     /locus_tag="ACZPFK_07940"
     CDS             460..1482
                     /locus_tag="ACZPFK_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1406280.1"
                     /translation="MIAYDKILGCLVGAAAGDAMGAATEIRTRAQIHELFGGDVRTFE
                     KPPKDTFARGSEPGQVTDDFSVAYVTARAVADNGGAVTEEVAKQALLEWAAVEKWFAR
                     FAGPTTRAHVAKLKGEAQPAADGFVPVNENGQSTNGAAMKSSPIALFSGGDLDKAIAD
                     ALTVCKLTHSNQIALSGAAAVAAATAAALREGANLFDVVQAGLYGAREGERLGGAVHT
                     NAGASVEKRMKMAIAIGLFARDMDEAIDQIGAYIGSGLMAAEAVPAVFGLMVAAKGNT
                     LDAICGGVNIGDDTDTVATMIGGILGALNGAGSLPAEYLGYLERQNGIELERLARDID
                     TLANRG"
     gene            1543..1992
                     /locus_tag="ACZPFK_07945"
     CDS             1543..1992
                     /locus_tag="ACZPFK_07945"
                     /inference="COORDINATES: protein motif:HMM:NF018184.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR1406281.1"
                     /translation="MEYEIVCRDAFAVAGVRRVTSEAGGVWRQLRAEGGLEALRRLAG
                     PGSPLLGLCFGFDEKGENDNMVGFLLRGEAPEGCEVCRYPACRWFVVTAEGKISDLTL
                     RRAWRWIHRELIDDGVICPRDAPTIEEYELWDEAADRCRVKLRVPVY"
     gene            complement(2378..>3200)
                     /locus_tag="ACZPFK_07950"
     CDS             complement(2378..>3200)
                     /locus_tag="ACZPFK_07950"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1406282.1"
                     /translation="LREGERVELYLNDEFFPSGAQKKAPPKKPSPLKIIYEDENIALL
                     YKPVHILCHSDRTGDANLVDWFVDALKEKGEYQPCGGSPFRPALCNRLDRGTEGLVLA
                     AKTYAALRDGTALVREDLLRKQYLCIAAGRPPEGTHTAYLVKDEAKNKVRIFRRPAEG
                     RKEIVTGVRVLEERQGLSLCEVTLFTGRTHQIRAHLTFLGCPLLGDKKYGDAQLNARF
                     PTLHDQALCAFRLGFAEDIPQGNSLYGLAGKVFELEDCRVLQLFGQIKTDGGCSG"
BASE COUNT          551 a          967 c         1143 g          539 t
ORIGIN      
        1 ctatgtgaat gggtgggagc ggtatatgca gcacacccac accgcatttt tcaacgagca
       61 cagcctggag ctgtttgaag agggcggcag tgaagaggcg ctgctgcata ggctgttcga
      121 tatgggggtg cagattgtgg tggagaccct gggcaaggag ggctgcgccg tcgtcacgcg
      181 gcaggagcgg ttcgccgtgc ccgcttacga tgtgccggtg gtggacacga ccggcgctgg
      241 ggacactttc aacagcagct ttgtctttgg cctgagccgg ggctgggccc tgggggagag
      301 cgcccgcttc gccaccgcgg cggccaatat ggccgtgatg gcccaggggc cgcgcagcgg
      361 catcaccagc gaggaaaaag tccgcgcatt tatgcggcag aacgcccatc tgctgggcgg
      421 caaaacgccg catatcgata caaaggaggt tggatcaaaa tgatcgcata cgacaagatt
      481 ctgggctgcc tggtgggggc ggccgccggg gacgccatgg gggcggccac cgagatccgc
      541 acccgggcac agatccacga gctgttcggt ggggacgtga ggacgtttga aaaaccgccc
      601 aaggacacct ttgcgcgcgg aagcgagccg ggccaggtga ccgacgactt ctcggtcgcc
      661 tatgtgaccg cccgcgccgt cgccgacaat ggcggcgcgg tgaccgaaga ggtggcgaag
      721 caggccctgc tggagtgggc ggccgtggaa aaatggtttg cccgcttcgc cggccccacc
      781 acccgcgccc atgtggccaa gctgaaaggg gaggcgcagc ccgccgccga cggctttgtg
      841 ccggtaaatg agaacggcca gtccaccaac ggggcggcca tgaaatcctc ccccatcgcc
      901 ctgttcagcg gcggggatct ggacaaagcc atcgcggacg cgctcaccgt ctgcaagctc
      961 acccactcca accagatcgc cctgtcgggc gcggcggctg tggccgccgc aaccgccgcg
     1021 gccctgcgcg agggcgcgaa cctctttgac gtggtgcagg cgggcctgta cggcgcgcgg
     1081 gagggggaac gcctgggcgg cgcggtgcac accaatgccg gggccagtgt ggaaaagcgc
     1141 atgaagatgg cgattgccat cggcctgttc gcccgcgata tggatgaggc catcgaccag
     1201 atcggcgctt acatcggctc cggcctgatg gccgccgagg cggtgcctgc ggtgtttggc
     1261 ctgatggtgg ccgccaaggg gaataccctg gacgccatct gcgggggcgt caacatcggc
     1321 gacgacaccg acacggtggc gaccatgatc ggcggcatcc tgggcgcgct gaatggcgcg
     1381 ggctcgctgc ccgcggagta cctgggctat ctggagcggc agaacggcat tgagctggaa
     1441 cggctggcgc gggacatcga caccctggca aaccggggct agagcgcggg atgcggcggc
     1501 ccgccgcaaa aaagtatatg tggcgcaaca ggagggcggc ggatggaata cgagatcgtt
     1561 tgcagggacg cctttgccgt ggccggcgtg cgcagggtga cgtctgaggc gggcggcgtc
     1621 tggaggcagc tgcgggctga gggaggcctt gaggctctgc ggcgtctggc cgggccgggc
     1681 agccctctgc tggggctctg cttcggcttt gacgaaaagg gtgaaaacga caatatggtg
     1741 ggttttctcc tgcgaggcga ggcgccggag ggctgcgagg tctgccggta tccggcctgc
     1801 cgctggtttg tggtcactgc ggaggggaag atctccgacc tgacgctgcg cagggcatgg
     1861 cggtggatcc accgtgagct gatcgacgac ggggtgatct gtccccgcga cgcgcccacc
     1921 atagaggaat acgagctctg ggacgaggcg gcggaccgct gcagggtaaa gctgcgggtc
     1981 cccgtctatt gaggccagct tcccacccca caacccagcc actctgcgcg gggcgaatgc
     2041 cggcgcaaag cgccggtctc cgcggggtcg ggttcgggcc gggggacatt tggcataatt
     2101 tcgaacggga tgcaaagcac cggcgacggt gcggtcaggt actgcgccct gcaggcgggc
     2161 ctattttggg ccgcggagcg gttttgaccg gacatctgcg catggctttg ggaaacgtcg
     2221 tttgaaaatc cgaaaaaagg ttggcggccc gaaagggtcg ccaacctttt ttgaataatg
     2281 gaatgcgctt ttgcgaccgc ggcacatggg agccgtgagg cggggggccg cgaccgcgtt
     2341 ctgccccaaa gcgccccgag acggcccctg ttctcagtca gccgctgcag cccccgtccg
     2401 ttttgatctg cccaaagagc tgcagcacac ggcagtcctc cagctcaaaa acctttccgg
     2461 ccagcccgta caggctgttc ccctgaggga tgtcctcggc aaagccgagc cgaaaggcgc
     2521 agagcgcctg gtcgtgcagg gtggggaagc gggcgttcag ctgcgcgtcg ccgtactttt
     2581 tatcccccag cagcgggcag ccgagaaatg tcaggtgcgc cctgatctgg tgggtgcggc
     2641 cggtgaacag ggtgacctcg cacagcgaga ggccctgccg ttcctccaac accctcacgc
     2701 cggtgacgat ctccttgcgg ccctcggccg ggcgacggaa gatacgcacc ttgtttttgg
     2761 cctcgtcttt gacgagatag gcggtgtgcg tcccctccgg cgggcggccg gcggcgatgc
     2821 agaggtattg cttgcgcagc aaatcttccc gcaccagggc cgtgccgtcg cgcagggccg
     2881 cataggtctt tgccgccagc accagcccct cggtgccccg gtccagccgg ttgcagagcg
     2941 ccggacggaa cgggctgccc ccgcagggct ggtattcccc cttttccttg agggcgtcca
     3001 caaaccagtc caccaggttg gcgtcgccgg tgcggtcgga atggcagagg atgtgcacgg
     3061 gcttgtagag gagcgcgatg ttttcgtcct catagataat cttgagcggg gagggctttt
     3121 ttgggggcgc ttttttctgc gcgccgctgg ggaagaactc gtcgtttaga tagagctcca
     3181 cccgctcgcc ctcgcgcagc
//
LOCUS       DBMDYA010000298         3189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6809_length_3189_cov_1.147345, whole genome shotgun sequence.
ACCESSION   DBMDYA010000298 DBMDYA010000000
VERSION     DBMDYA010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6809_length_3189_cov_1.147345"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(274..999)
                     /locus_tag="ACZPFK_07955"
     CDS             complement(274..999)
                     /locus_tag="ACZPFK_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR1406283.1"
                     /translation="MERPEKKRRFTPRGLLSLGLILAGAALLLYYVGVNWVPKWYREW
                     TYRENLDMSLPTVTDLSLAEDGGFVEDNYSRYTYPTDSFFITSTRKAYESGQMVLRVP
                     RLAYEGEVLDGTTEGVLKHGVGLYNYSALPSYGNPNVCIAGHRGVYGAEFYNIDKFEE
                     GDLLYLEYDGYLFTYQYIETVTVPVDDWGMMYCTDYSAITLSSCQMEGDTERICVRGK
                     LLKAEPLEQQGEGASSSAASSGG"
     assembly_gap    1229..1238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1303..2508
                     /locus_tag="ACZPFK_07960"
     CDS             1303..2508
                     /locus_tag="ACZPFK_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1406284.1"
                     /translation="MQQPCIRIAMLGEFRVTVGDAVVSGGGARANQIWNLLQYLVAFR
                     HKTLSQEEIIDAMWEEGTGDPASALKNRVYRIRTAFAKAGAPYAKDVIRLSGGVYAWN
                     NDLPCQVDTEIFEDCCARAAEPSLPQAQRIALYRQAVDLYKGDFLPAAYFMSWVVPLS
                     RHYHTLYFKSVQSLLELYEATQDYEAMYEVADSASGIDKFEETPHKYILLSLMGQGKQ
                     AQALNYYNYVSDLFYREMGVGLSEELRALYQEIAKTAKTARTDLDVLREDMREDPSSI
                     NGAFYCEYEVFKNLYRIEARAAGRHGSSTYLGLLGLSAPEGGEPSREALERGMEALYR
                     CIMDSLRRGDVFSRCSASQYVLLLSPITYEDGQVVLTRIEKNFKRIYHSRKVALVRNL
                     QPLIPVDEW"
     gene            complement(2879..>3189)
                     /locus_tag="ACZPFK_07965"
     CDS             complement(2879..>3189)
                     /locus_tag="ACZPFK_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406285.1"
                     /translation="PLGMMTALPSMLATHIPPFLCRMAGPGGTTTPFAGFIIVIISRC
                     SHRPVTVFTFAVTTFSKFSRARDQSHRILFDCFIFLKLDPCKTLYLRLFSIFGENHNF
                     "
BASE COUNT          629 a          978 c          961 g          611 t
ORIGIN      
        1 ttttgtgggg tcccgcccgg cgcagcccgc ccgggcgggc gaaaagccga agagcgccgg
       61 cgcagagcgc ctttttgccg gggcggggcg ggaggtcggg cggcccgcac cgccccggcg
      121 gtttgctttt agtgtacatc aattttgaaa aatggcaaca aaaatttatc gaccgccgcc
      181 gcggcgcgcc gttttgccct gcggccgctc aaccgccgcc gccgtgaccc ggcggcaggg
      241 gcaccctttc cccgggtttc gctcttgggc cgctcagccc ccgctgctgg cggcagagga
      301 ggaagctccc tcgccctgct gctccagggg ctcggctttc agcagcttgc cgcgcacgca
      361 gatgcgctcg gtgtcgccct ccatctggca gctggaaagg gtgatggcgc tgtagtcggt
      421 gcagtacatc atgccccagt cgtccaccgg cacggtcacc gtctctatat attgataggt
      481 gaacaggtag ccgtcgtatt ccagatacag caggtcgccc tcttcaaact tgtcgatgtt
      541 gtagaactcg gccccgtaga cgccccggtg cccggcgatg cagacgttgg ggttgccgta
      601 gctgggcagg gcgctgtagt tgtacagccc caccccgtgc ttgagcaccc cctcggtggt
      661 gccgtccagc acctcgccct cataggccag ccgcggcacc cgcagcacca tctgcccgct
      721 ctcgtaggcc ttgcgcgtgc tggtgatgaa aaagctgtcg gtggggtagg tgtagcggct
      781 gtagttgtcc tccacaaagc cgccgtcctc cgccagcgac aggtccgtca ccgtgggcag
      841 cgacatatcc aggttttcgc ggtaggtcca ctcgcggtac cacttgggca cccagttgac
      901 ccccacatag tacagcagca gcgcggcgcc cgccaggatg aggcccagcg acagcaggcc
      961 gcgcggggtg aagcgacgtt ttttttcggg gcgttccatg atctgtcctc ctgacctccg
     1021 tccgttttta aaatttcacc tttcttgaaa actgaaaaag gtaaaagtca taaaatatca
     1081 gaggagtttt acaaaataac gaaaagatga tgaaaaaaca gaagcaatcg gataaaatgc
     1141 acaaaagaat gtttttattt tcatccaaac tttgaaatag ttagcgcttt ctattttacc
     1201 gcccatttgg tatacttgta aatagcgann nnnnnnnnaa tttttttgag accgattttc
     1261 acagaccgat acgagaccga tagaaaaggg gggtggggcg gtttgcagca gccttgtatc
     1321 cgtatcgcca tgttgggcga gttccgggtg acggtggggg acgcggtcgt cagcggcggc
     1381 ggcgcccgtg caaaccagat ctggaacctg ctgcaatacc tggtcgcttt ccggcacaag
     1441 accctctcgc aggaggagat catcgacgcc atgtgggagg agggcaccgg cgacccggcc
     1501 agcgcgttga aaaaccgggt ctaccgcatc cgcaccgcct ttgcaaaggc gggcgcgccc
     1561 tatgcaaaag acgtcatccg cctgtcgggc ggcgtctacg cctggaacaa cgacctgccc
     1621 tgccaggtgg ataccgagat ttttgaggac tgctgcgccc gcgccgccga gccctcgctg
     1681 ccgcaggcgc agcgcatcgc cctttaccgc caggcggtgg acctgtacaa gggcgatttt
     1741 ctgcccgcgg cttatttcat gtcctgggtc gtcccgctga gcaggcatta ccacaccctt
     1801 tatttcaaaa gcgtgcagag cctgctggag ctgtacgagg ccacgcagga ctatgaggcc
     1861 atgtacgagg tggccgacag cgcctcgggc atcgacaagt ttgaagagac gccccacaag
     1921 tacatactgc tcagcctgat ggggcagggc aagcaggcgc aggcgctcaa ctattacaat
     1981 tacgtcagcg acctgttcta ccgcgagatg ggggtgggcc tgtcggagga gctgcgcgcc
     2041 ctgtaccagg agatcgccaa gaccgcaaag accgcccgca ccgacctgga cgtgctgcgc
     2101 gaggacatgc gggaggaccc ctccagcatc aacggcgctt tttactgcga gtacgaggtc
     2161 ttcaaaaacc tttaccgcat cgaggcgcgg gcggcggggc ggcacggctc ctccacctat
     2221 ctgggcctgc tgggcctgag cgcgccggag ggcggcgagc cctcccgcga ggcgctggag
     2281 cggggcatgg aggcgctcta ccgctgtatc atggacagcc tgcgccgcgg cgacgtgttc
     2341 tcccgctgca gcgccagcca gtatgtgctg ctgctctcgc ccatcaccta tgaggacggc
     2401 caggtcgtgc tcacccgcat cgagaagaat ttcaagcgca tctaccactc gcgcaaggtg
     2461 gcgctggtgc gcaacctgca gcccctcatc ccggtggacg agtggtgagc gcgggcgagc
     2521 gcgggcgcaa aagcccgccg ctttctgcag accctttctg cgtagatttt ttggaagagc
     2581 gcggccccgc ggggccgcgc tcttttgagg gggcggggca aaaggccccg gcgcgggcgg
     2641 cccgggcagc cccctacggc ggccggttcc tccccggcga ccgttcctcc ccggcggccg
     2701 tccctctccg acagccgtcc ctctccggcg tcgcccctcc tccccggtga cggatttcct
     2761 ctccggcggc cgcagaagcc gcgtcttgct gccgcccccc gcgccgcggg ttttcccgca
     2821 ggtttccctc aggaagccgg ccacagaaaa gggatcgtca ttttgccatt ttttcaattt
     2881 aaaaattgtg attttcccca aaaatagaaa aaagtctcaa ataaagcgtt ttacaggggt
     2941 ctaatttcaa aaaaataaag caatcaaata aaattctgtg actttggtcg cgggcacggg
     3001 aaaattttga aaaagttgtg accgcaaaag taaaaacggt gactggacgg tgactgcacc
     3061 gtgagataat gacaatgata aaaccggcga aaggggtcgt tgtcccccca gggccggcca
     3121 tccgacacag gaaaggcggg atgtgtgtgg cgagcatcga cggcaaagcc gtcatcatac
     3181 ccaacggcg
//
LOCUS       DBMDYA010000299         3172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6855_length_3172_cov_2.098991, whole genome shotgun sequence.
ACCESSION   DBMDYA010000299 DBMDYA010000000
VERSION     DBMDYA010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6855_length_3172_cov_2.098991"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..3001
                     /locus_tag="ACZPFK_07970"
     CDS             20..3001
                     /locus_tag="ACZPFK_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR1406286.1"
                     /translation="MINQQKNGDKYKRQYWDKVGPCIHTRNDQLASQNTIHPSDDRVF
                     SIRELMLMMTVPHSFKWAETDFSDLNGLPPDGKRAFLKKEEIKIRQCLGEAVPTVIFQ
                     SIAKKIKGALSCPPLNTAAIRRLVACQGLTDITALKKFVSDNPLGLSSAALSKIAESA
                     NTRRTDNAAFFTSKTLITEMIKALPGTDKETVRILEPSVGVGNFIPLILRKFEGKNIV
                     LDVVDIDKHSLDLAKLILERDKIPDRCTINFIHTDFLLCDPKEKYDYVIGNPPFYKMK
                     ASDPLLSLYRKSAVNTATTNICSFFLDKALQMGNYIALVFPKFLLNTPEFAQTRKHLS
                     HKAVECIIDFGEKGFPGVLVETVAIFIRNGERPSSTRVLSIPHGKSMTQPQNYIFDEK
                     LPYWIIYRDGEFDAVCKKLDFNVFKVFRDRQITNELLSPKGEIRVLKSRNISDDGKKV
                     LDIEGYDAYISRADAQGLSVYSYLDCDGVYLTPNMTYKPRVMKKPKGCLVNGSLAVLI
                     PQKGITPTEKQMAFFSTQEYRRFYQVARNFQTRSLNVDACSVFFYGLLREAPKKLPIT
                     EKAEEKAQVAVFEGRKGWRNMLTQQDIINFCAQHNYDIRESRNGRWIDQKCAADVVTV
                     IADCIYNYALAHPQAIFTTLDIWHSAYAVENVESIFKKPGVKSETAKNEYDKFFQQPM
                     ELLAYAQVLKKIKRGNRNFYQINNIEVLEYIALRERNALFFLKTYIEKVLLDSGLYRA
                     FEAFFAAPTKGSYQEVKQTFSSFLLCNTKINGVVECNRIFIKVLNPLAYFKNSRGTEK
                     GRLSAQPITYDMLMYNRNNFRDIYADKPKGIARKEYALTHPVKVNKAYYHYQSAKAKK
                     YLRMFNDQNRRGRTEHPEAAHSKDRAIHMHHIFPEADYPEIGSYLENIIALTPTQHLN
                     YAHPNGHTQEIDEQYQHLLLLAKADRIRENLTGTAADKIYEFSNFLFVLHVGFDSDEV
                     LEIEEMDFDSVVNAINVHYAA"
BASE COUNT          892 a          823 c          765 g          692 t
ORIGIN      
        1 ggtcatcggg ggtaaaattg tgatcaacca gcaaaaaaac ggcgacaaat acaaaaggca
       61 atactgggat aaggtcggcc cctgcatcca caccagaaac gatcaattgg caagccaaaa
      121 cacgatccac cccagcgatg accgggtgtt cagcatccga gagctgatgc tgatgatgac
      181 ggtcccccac tcctttaaat gggccgaaac ggatttttca gatctaaacg gcctgccgcc
      241 ggacgggaaa cgggcatttt taaaaaaaga agaaataaaa atccggcaat gcctggggga
      301 agccgtcccc accgtcattt ttcaatccat agcaaaaaaa ataaagggcg cgctgagctg
      361 tcccccgctc aacacggcgg cgatccgcag attagtggcc tgccaagggc tgaccgacat
      421 cacagcttta aaaaaatttg tctcggataa tcctctgggc ctgtcgtcgg ccgcattgag
      481 caaaattgcc gaatcggcaa atacgcgccg gacggataac gcggcgtttt tcaccagcaa
      541 aaccttgatc actgagatga tcaaggctct gccgggcacc gacaaagaga cggtgcggat
      601 cctggagccc tccgtcggag tggggaattt tatcccgctt atcctcagaa aatttgaggg
      661 caaaaatatt gttcttgacg tggtggatat tgataagcac agcttggacc ttgccaaatt
      721 gatactggag agggataaga tccccgatcg ctgcacgatc aactttatcc acacggattt
      781 tttgttgtgc gatcccaaag aaaaatacga ctatgtcatc gggaacccgc ccttttataa
      841 aatgaaagca agcgaccccc tgctgagcct ctatcgaaaa agcgcggtaa ataccgcgac
      901 caccaatatc tgctctttct ttttggacaa ggcgcttcag atggggaatt atatcgcctt
      961 ggtgttccct aaatttctgc tcaacacgcc tgagtttgcg caaacgagaa aacatttgtc
     1021 ccacaaggcg gtggagtgca ttattgactt tggcgaaaag ggcttccccg gtgtgttggt
     1081 agagacagtg gcaattttca tccgcaacgg ggagcgtccc tccagcaccc gcgtcctctc
     1141 aattccccac ggaaaatcca tgacacaacc gcagaactat atttttgatg aaaaactgcc
     1201 ctattggatc atttatcggg acggcgaatt cgatgccgtc tgcaaaaagc tcgatttcaa
     1261 cgtcttcaag gtattccgcg acaggcagat aacgaatgag ctgctttccc caaaaggaga
     1321 gatccgcgtc ctcaaatccc gcaatatcag cgacgacgga aaaaaagtgc tggatataga
     1381 gggctatgat gcgtatatct cccgtgcaga cgcccaaggc ctgtcggttt acagctacct
     1441 tgactgcgac ggcgtgtatc tcacccccaa tatgacttat aagccccggg tgatgaaaaa
     1501 gcccaaagga tgcctggtaa acggctctct ggcggttctg atccctcaaa aaggaatcac
     1561 gcccaccgaa aaacaaatgg cgtttttctc gacgcaggag tacaggcgct tttatcaggt
     1621 cgcaaggaac tttcagacca ggtcgttaaa tgtggacgcg tgttcggtgt tcttttacgg
     1681 gctgttgaga gaggcgccta aaaagcttcc catcaccgaa aaagcggagg aaaaggctca
     1741 agtcgccgtc tttgagggga ggaaagggtg gagaaacatg ttgacacagc aggacatcat
     1801 caatttttgc gcccagcaca attacgatat aagggaaagc cgcaacggca ggtggatcga
     1861 ccaaaagtgc gcggcggatg tggtcaccgt gatcgccgac tgcatttata actatgcgct
     1921 tgcgcatccg caggcgatct ttacgacgct ggatatctgg cattccgctt atgccgttga
     1981 aaacgtcgaa tccattttta aaaagcccgg cgtcaaaagt gaaaccgcca aaaacgaata
     2041 cgacaaattt tttcagcagc ccatggagct gttggcctat gcgcaggtgc tgaaaaaaat
     2101 caagcgcggc aatcgaaact tttaccagat caacaatata gaggtattgg aatatattgc
     2161 cctgcgcgag aggaacgcct tgttttttct gaaaacctat attgaaaagg tcctcttaga
     2221 cagcgggctc taccgggctt ttgaggcgtt ttttgcagcg ccgacgaaag ggtcttacca
     2281 agaggtgaag cagacctttt caagctttct cctctgcaat acaaaaatca acggggtggt
     2341 ggagtgcaac cgcatcttca tcaaggtttt gaaccccctg gcgtatttca aaaattcccg
     2401 cggcactgaa aagggcaggc tgtccgcgca gcccatcacc tatgacatgc tgatgtacaa
     2461 tcgcaataac ttcagggata tttatgcaga caagcccaag gggatcgcca gaaaagagta
     2521 tgccctgacc catcccgtca aagtgaataa agcctattac cactatcaat ccgccaaagc
     2581 aaaaaaatat ctcaggatgt ttaacgacca aaaccgcagg ggccgcaccg agcatcccga
     2641 ggccgcgcac agcaaagacc gggcaatcca catgcatcat atctttcccg aagcggacta
     2701 ccctgaaatt ggttcctatc tggaaaatat catcgccctc acgccgacac agcatttgaa
     2761 ttatgcccat cccaacgggc acactcaaga gatcgacgaa caatatcagc acctgctgtt
     2821 gctggccaag gcggacagga tccgcgaaaa cttaacgggt accgccgcgg ataagattta
     2881 tgaattctcc aatttccttt ttgtcctcca tgtcggtttt gacagcgacg aggtgctgga
     2941 aatcgaagag atggatttcg attcggtcgt caacgccatc aacgtccact acgccgcata
     3001 gcgcaaacgg gaagcgccgc cggtctccgg cgcccattcc tcctgcaccg cagcggcaaa
     3061 agcaattaca agttttttgt gaaaagccct gcccgatggc cgttaaaaga gaaaacactg
     3121 taaacccaaa agaagcgcct ctacccgagg cgcctctttt ggtttattta ca
//
LOCUS       DBMDYA010000300         3171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6857_length_3171_cov_1.300326, whole genome shotgun sequence.
ACCESSION   DBMDYA010000300 DBMDYA010000000
VERSION     DBMDYA010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6857_length_3171_cov_1.300326"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="ACZPFK_07975"
     CDS             <1..319
                     /locus_tag="ACZPFK_07975"
                     /inference="COORDINATES: protein motif:HMM:NF037182.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1406287.1"
                     /translation="YPPFCGLCMVQFSGAEERETADAARAFGAYLKEGAAGRGDLPLR
                     VLGPAPAGVAMLHGRYRYKLTIKCRNDGAFRELLRQVQRRYSDSPYVGKISVSIDFNS
                     DSD"
     gene            371..910
                     /gene="def"
                     /locus_tag="ACZPFK_07980"
     CDS             371..910
                     /gene="def"
                     /locus_tag="ACZPFK_07980"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1406288.1"
                     /translation="MALRTIVQDGDPILKKVCRPVTEFNARLAQLIDDMKETLVDACG
                     LGLAAPQVGVMRRVCIVVDLPPEDDGAAPEEGEEPEYRFIELVNPEIIDRQGSVGLYE
                     GCLSFPGHNGYIERPEQVTVRAFDRDGNEFTATRTGMTARAICHECDHLDGVTIMELA
                     EYFYEDQEPEEDAEEDAGE"
     gene            936..1862
                     /gene="fmt"
                     /locus_tag="ACZPFK_07985"
     CDS             936..1862
                     /gene="fmt"
                     /locus_tag="ACZPFK_07985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1406289.1"
                     /translation="MRLVFMGTPDIAAAALEALIGAGHEIAGVFTREDKPVGRKQVLT
                     PPPVKVLAQRHGIPVFQPKTLRDGEAERILRGLAPELIAVVAYGRILPKEILALPRFG
                     CVNLHVSLLPKYRGAAPIQWAVLNGETETGVTIIQMDEGMDTGDILAVAPVEIGPEET
                     SADVFEKVTALGAETLARTVAAIGAGRVRAVPQEHEKATSAPPLKKEMAAFDFSQDAK
                     KLHNLIRGLYPWPVAQFTAGGVKVKVNRARAAAGVGAPGEVLSLKPLTVACGAGALEL
                     LEVVPQGKRPMTGQEWAAGRRLKKGDFLRPEV"
     gene            1937..2635
                     /locus_tag="ACZPFK_07990"
     CDS             1937..2635
                     /locus_tag="ACZPFK_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR1406290.1"
                     /translation="MYFYGIDVYYIVLVVPALVFAMWAQMRVSSAFNRYSQLRTGGGL
                     TGADAARLILDRNGLTDVAVELTEGRLSDHYDPRAGVIRLSREVYYNATVAAVGVAAH
                     ECGHAVQHAEGYFPLKVRSAIIPVTQIGSNLAFPLFLLGMLFSYPPLMDAGILLFALV
                     AVFQLVTLPVEYNASSRAVATLSGSGMVSAEEERGVRKVLSAAALTYVAALATALANL
                     LRLILIAGRRNNRN"
     gene            2646..>3171
                     /locus_tag="ACZPFK_07995"
     CDS             2646..>3171
                     /locus_tag="ACZPFK_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013216.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1406291.1"
                     /translation="MQYPRRLAAEALIRIERGGYSNLVLAGLLRDSGFSPRDRAFCSR
                     LVYGVLERRRLLDARLAPFLKKPVEKLDAPVRAALRLGLYQQLYMDAVPSAAAVNESV
                     KLVRALGKSSAAGLVNAVLRRAAGPWQPIFHSEVERLGVTCSVSDQIAALFLDAFGPQ
                     AEPLLAAAFEPPALA"
BASE COUNT          518 a          980 c         1153 g          520 t
ORIGIN      
        1 ctacccgccg ttctgcgggc tgtgcatggt gcaattttcc ggcgcggagg agcgggagac
       61 cgcggacgcc gcccgcgcct ttggggcata cttgaaagag ggggcggcgg gccgggggga
      121 cctgccgctg cgggtgctgg gccccgcgcc cgccggtgtg gccatgctcc acggccgcta
      181 ccgctataag ctgaccatca agtgccgcaa cgacggcgct ttccgcgagc tgctgcgcca
      241 ggtgcagcgg cgctacagcg acagccctta tgtgggcaag atatcggtga gcatcgattt
      301 caacagcgac agcgactgag cggcgctgtc ccacgttttt tgacagcttg agtgaaggag
      361 acgagaagag atggctttga ggacgatcgt acaggatggg gaccccatcc tgaaaaaggt
      421 ctgccgcccg gtgacggagt tcaacgcccg gctggcgcag ctgatcgacg atatgaaaga
      481 gacgctggtg gacgcctgcg ggctgggctt ggccgcgccc caggtggggg tgatgcgccg
      541 ggtgtgcatc gtggtggacc tgccgccgga ggacgacggc gccgcgccgg aggaggggga
      601 ggagcccgag tatcggttta tcgagctggt caaccccgag atcatcgacc gccagggctc
      661 ggtggggctg tacgagggct gcctgtcttt ccccggccac aacggctata tcgagcggcc
      721 cgagcaggtc acggtgcggg cgtttgaccg ggacggcaac gagttcaccg ccacccgcac
      781 cggcatgacc gcgcgggcca tctgccatga gtgcgaccat ctggacggcg tgacgatcat
      841 ggagctggcg gagtatttct atgaggatca ggagccggag gaagacgcgg aagaagacgc
      901 gggggaatga ccccgggaga ggggcgcagc aggatatgag actggtattt atggggacgc
      961 cggacatcgc cgccgccgca ctggaagccc tgatcggcgc agggcacgag atcgcgggcg
     1021 tcttcacccg tgaggacaag ccggtggggc gcaaacaggt gctcacgccg ccgccggtga
     1081 aagtgctggc gcagcggcac ggcatcccgg tctttcagcc caaaacgctg cgggacggcg
     1141 aggcggagcg catcctgaga ggactggccc ccgagctgat cgccgtggtg gcgtacgggc
     1201 gcatcctgcc gaaagaaatt ttggccctgc cgcgctttgg ctgtgtcaac ctgcacgtgt
     1261 cgctgctgcc gaaataccgg ggcgcggccc cgatccagtg ggcggtgctg aacggcgaga
     1321 ccgagacggg ggtcaccatc atccaaatgg acgagggcat ggacaccggg gatatcctgg
     1381 cggtggcccc ggtggagatc gggccggagg agaccagcgc cgacgtgttt gaaaaggtaa
     1441 cggccctcgg ggccgagact ctggcgcgga ccgtggcggc catcggcgcg gggcgggtgc
     1501 gggccgtccc gcaggagcat gaaaaggcga cgtcggcccc gccgctgaaa aaagagatgg
     1561 cggcctttga tttttcacag gacgcaaaaa agctgcacaa cctgatccgt ggcctttatc
     1621 cctggccggt ggcccagttc acggccggcg gcgtcaaggt caaggtgaac cgggcgcggg
     1681 cggcggcggg cgttggcgcg ccgggcgagg tgctgtcctt aaagcccctc accgtggcct
     1741 gcggcgcggg cgcgctggag ctgctggagg tggtgcccca gggcaaacgc ccgatgaccg
     1801 ggcaggagtg ggcggccggc cgccgcctga aaaagggtga ttttctgcgg ccggaagtgt
     1861 gaagcgcacc ccggcgggcg cagcccccac agggacgatc ccgggccgcg cagctcttga
     1921 ataggaggca aactgtatgt atttctacgg catcgacgtt tattatattg tgctggtggt
     1981 cccggcgctg gttttcgcca tgtgggcgca gatgcgagtc agctcggcgt tcaaccgcta
     2041 ttcccagctg cgcaccgggg gcggcctcac cggcgccgac gcggcacggc tcatcctgga
     2101 ccgcaacggt ctgaccgacg tggcggtgga gctgaccgag gggcggctca gcgaccacta
     2161 cgacccgcgg gcgggggtca tccggctctc ccgcgaggtc tattacaacg ccacggtggc
     2221 cgcggtcggc gtggccgccc acgagtgcgg gcatgcggtg cagcacgccg agggctattt
     2281 cccgctcaag gtgcgcagcg ccatcatccc ggtgacccag atcggctcca acctcgcctt
     2341 tccgcttttt ctgctgggta tgctgttttc ttatcctccg ctcatggacg caggcatcct
     2401 gctgttcgcc ctggtggcgg tgttccagct ggtgaccctg ccggtggagt acaatgccag
     2461 cagcagggcc gtggcaaccc tgtcgggcag cggcatggtc tcagcggagg aggagcgcgg
     2521 cgtgcgcaag gtgctctcgg cggcggcgct cacctatgtg gcggcactgg ccaccgccct
     2581 ggcgaacctg ctgcgcctga tcctgatcgc gggccgccgc aacaaccgga actaggggga
     2641 tgcagatgca atatccacgc cgcctggcgg ctgaggcgct gatccgcatc gagcgcgggg
     2701 gttattccaa tctggtgctg gcgggcctgc tgcgggacag cggcttttcc ccccgcgacc
     2761 gtgccttttg ctcccgtctg gtatacggcg tgctggaacg acggcgccta ctcgacgcga
     2821 ggctcgcgcc ctttctcaaa aaaccggtgg aaaagctgga cgccccggtg cgggcggcgc
     2881 tgcggctggg gctgtatcag cagctctata tggacgcagt gccaagcgcc gctgcggtga
     2941 acgagtcggt aaagctggtg cgggcgctgg gcaaaagctc ggccgcgggg ctggtgaacg
     3001 cggtactgcg ccgcgccgcc gggccctggc agccgatctt tcacagcgag gtcgagcgcc
     3061 tcggcgtcac ctgctcggtc tccgaccaga tcgcggccct ctttttggac gctttcggcc
     3121 cgcaggcgga accgctgctt gcggccgctt ttgagccgcc tgcgctcgcg g
//
LOCUS       DBMDYA010000301         3167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6868_length_3167_cov_1.522505, whole genome shotgun sequence.
ACCESSION   DBMDYA010000301 DBMDYA010000000
VERSION     DBMDYA010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_3167_cov_1.522505"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(19..777)
                     /locus_tag="ACZPFK_08000"
     CDS             complement(19..777)
                     /locus_tag="ACZPFK_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1406292.1"
                     /translation="MNNNRIEQIYKLLQIHEQISVQDLADLLQVTKTTIRRDLLLMEE
                     KGLVRRRRGFALLPTRDLLTRFNPSDLFQEEKMRIAKKAARYVTSHTSLALDSGSSVS
                     ALVKCLKDDPTIQGLDIVTSSLAIAIATCEQYRVAIPGGFVLAGEHCVGGVDVTDFFH
                     KIHADPVFLGSTGVSNSNGLTVSYPLMRSVKEALVGCATTRIALLDSSKFHSRGLYTF
                     CDFDALDILITVENEENAPLLEEIARHHVEVVLA"
     gene            1563..1844
                     /locus_tag="ACZPFK_08005"
     CDS             1563..1844
                     /locus_tag="ACZPFK_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406293.1"
                     /translation="MGLSVLGAYPHSMGRLQGFKTVGRDGPHCRFYRFAVQSCKNSGS
                     TDYRLAVFLPQRKAFGSTTGLPFKPVLFCLKADEKGDVALLCSGILPKE"
     gene            1863..3152
                     /locus_tag="ACZPFK_08010"
     CDS             1863..3152
                     /locus_tag="ACZPFK_08010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406294.1"
                     /translation="MKKAKTGKLHYAWWIMFACICMKIGSSAAVAAAMSNFITPIVRD
                     LGCEVSALTTFVSIEAVAMALMYTTASKILTTKKIGRVMGIASIVEIGGLALMSTYHS
                     VPMFYFSGLLIGVAQAFTGFVAIPIIINMWFRKKAGTVLGIVVAVSGAAGILYNLLSA
                     QLITAFGWRTAYLVLAAMAAVLTLPAVFLIIKSPAEVGCLPYGAEDADSVEPEKRADW
                     AASEWGLTRKQAFSSLFLYIAWLACIMYSYGSGVSGYTATYATMELGQSINFGAFAGI
                     ALSVGTIVSSLILGWINDRLGVRAGLVWGAATTALGYAVMLASKANPVLVLPAAFTVG
                     LGASMYTVQCPLLARSIVGVKHYSEIWSLMMMANSLIGGGLYSSIGLFYDKLGSYTGA
                     FVMAIVLYVGAFLVGALAISLARRHRKEEQEALRQKV"
BASE COUNT          719 a          794 c          969 g          685 t
ORIGIN      
        1 aggagaaagc aaaaaagact aggccaggac gacctccaca tggtgccggg caatctcttc
       61 cagcaggggg gcgttctcct cattttccac ggtgatgagg atgtccagcg cgtcaaaatc
      121 acaaaaggta tagagcccgc gggagtggaa ttttgaggaa tccagcaggg cgatcctggt
      181 ggtggcacag cccaccagcg cttcctttac cgagcgcatg aggggataag acacggtgag
      241 cccattgctg ttggagacgc cggtggaacc taaaaagacc gggtcggcat ggattttatg
      301 aaagaaatca gtcacgtcga caccgcccac acagtgttcg ccggccagca cgaagccgcc
      361 ggggattgcc acccgatatt gctcgcaggt ggcgatggcg atggccagcg aggaggtgac
      421 gatgtccagc ccctggatgg tgggatcgtc tttcaggcat ttgacgagcg cggagacgct
      481 ggagccggag tccagcgcca gcgaggtgtg ggaggtgaca tagcgggccg cctttttggc
      541 gatgcgcatt ttttcttcct gaaagagatc gctggggttg aaccgggtca acagatccct
      601 ggtgggcagc agcgcaaagc ccctgcggcg gcgcaccagc cccttttcct ccatcaaaag
      661 gaggtcgcgc cggatcgtcg tcttggtgac ctgcagcaga tcggccaggt cctgcacgga
      721 aatctgttca tggatctgca gcagcttgta gatctgttcg atcctgttgt tgttcatagc
      781 ggcctccccg agagagaaaa gagtgaaaaa tgtcttatcc cttttactat cctagtggcg
      841 tctgttcttt ttgtcaataa aaagagaaaa acgaacacga aacgataaaa aagaacaaaa
      901 agaaaaacag tttaacaaag cggttgagca cgacaggggg aaagctgttt tagagctttg
      961 ctgtgtcggg gaaaatcgga aaggtgagag acaagttttt attgttgcgc aataaaaaac
     1021 ggcttatctc gatagctctg ttttcccgcg cggccggaca accggaacgg agggcacaaa
     1081 gcagccagcg gccgcttgga ttgcctgatg gagagggcgg gtataacggg gcgtcggtgg
     1141 gataagactt ttgcaaaaag caggacaaga cgtttttggg cggcagctcc gcagaacttt
     1201 gaaaatgctt tttggcacgt gaaacaagta gaaaaaggct tcggcaaaat agggaaaaac
     1261 aagttgctct tttgtacaag tttatgtaga aacgaacact caaagcgaac aaatgcgacg
     1321 acttttatat tttaaagcga aaaagattga aaaaagggga aagtgtaata tgataaaaat
     1381 agccgcaggc tatttttcgg ccatccgttt cggttgccgc gacagcggcg agaaacgggc
     1441 catcaagatc agttgttttt tattgcgaag caataaaaaa cagccgcagc gaagcgtagg
     1501 ctattttttc ggctatcgat tttggctgtc tgctttggcg cagcagggcg gagcgcggcg
     1561 caatgggttt gtctgttttg ggtgcatatc ctcactccat gggccgactc cagggtttta
     1621 agaccgtggg cagggacggc ccgcactgcc gcttttatcg ctttgccgtg caaagctgca
     1681 aaaacagcgg cagtaccgac tacaggctcg cggttttttt accgcagcgc aaagcattcg
     1741 gctctacgac aggtttgcct ttcaaacctg ttttattttg tttgaaagcg gacgaaaaag
     1801 gggatgtcgc ccttttgtgt tccgggatat tgccaaaaga ataagccata agggtgagga
     1861 caatgaaaaa agcgaaaaca ggcaagctgc attatgcctg gtggatcatg tttgcctgta
     1921 tctgcatgaa gatcggctcc agcgcggcgg tggcggcggc gatgagcaac ttcatcaccc
     1981 ccattgtgcg ggacctggga tgtgaggtga gcgcgctgac cactttcgtc agcatcgagg
     2041 cggtggccat ggcgctcatg tacaccacgg cctccaagat actgacgacc aaaaaaatag
     2101 gcagggtgat gggcatcgcc tctatcgtgg agatcggcgg gttggcgttg atgtccacct
     2161 atcactctgt tcccatgttt tacttttccg gcctgctgat cggcgtggcg caggcgttca
     2221 ccgggtttgt cgccatcccc atcatcatca acatgtggtt ccgcaaaaag gcgggcacgg
     2281 tgctgggcat tgtggtggcg gtcagcggcg cggcgggcat cctttacaat ctgctgtcgg
     2341 cacagctgat cacggctttc ggctggagaa cggcttatct ggtgctcgcc gccatggccg
     2401 ccgtgctcac gctgccggcg gtattcctga ttatcaagag cccggccgag gtcgggtgcc
     2461 tgccctacgg cgcggaggat gcagacagcg ttgagcccga aaagcgcgcc gattgggccg
     2521 cctccgagtg gggcctcacg cgcaaacagg cattcagctc cctgttcctc tacatcgcct
     2581 ggctggcctg catcatgtac agctatggca gcggcgtgtc gggctatacc gccacctatg
     2641 ccacgatgga gctggggcag tcgatcaact tcggcgcttt tgcgggcatc gccctcagcg
     2701 tcggcaccat cgtcagcagc ttgatcctgg gctggatcaa cgaccgcctg ggggtgcggg
     2761 cgggtttggt ctggggggcg gcgaccacgg cgctggggta tgcggtcatg ctggccagca
     2821 aggcaaaccc cgtgttggtc ctgcctgcgg ctttcacggt cggcctgggg gccagcatgt
     2881 atacggtgca gtgcccgctg ctggcccgca gcatcgtggg cgtaaagcat tactctgaga
     2941 tctggtcgct gatgatgatg gcaaacagcc tgatcggcgg cgggctctac tcttccatcg
     3001 gcctgttcta cgacaagctg ggcagctata ccggggcttt tgtcatggcc attgtgctct
     3061 atgtgggggc gttcctcgtg ggtgccctgg cgatctcgct cgcgcgcagg cacaggaaag
     3121 aggaacaaga agcgctgaga caaaaagtat aaaaaacgga ggcggaa
//
LOCUS       DBMDYA010000302         3165 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6876_length_3165_cov_1.557441, whole genome shotgun sequence.
ACCESSION   DBMDYA010000302 DBMDYA010000000
VERSION     DBMDYA010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_3165_cov_1.557441"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="ACZPFK_08015"
     CDS             complement(<1..407)
                     /locus_tag="ACZPFK_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406295.1"
                     /translation="MQVTGRDNNMKIGFESMPGIAEALLETLSEKKLSEAVPDELVQG
                     LERVVVTGCGDSYAATLATKELNERMFAGCDYHALRTIDVSRHFVFPQRAPEKTLVMV
                     ISVSGGGARVTEAMMRAAKAADIPVFIVGHVNK"
     gene            complement(398..1357)
                     /locus_tag="ACZPFK_08020"
     CDS             complement(398..1357)
                     /locus_tag="ACZPFK_08020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1406296.1"
                     /translation="MIEYLACGNVMSDEVVQPDGFHSERHMGGPAFFALSGIRLWTKS
                     CKLVSSVGADFDDTYGAWMRNNGVSQESVRVEAEHTSCYELSYNEDGSGRGARSYYGE
                     ENLGYLKTHPDRIDDAATRDVKGIYMAQGADRVIWKKLEGLKIKYGFQIMWELEYFNS
                     GRDLERVKEVMPIADMWSLNYNEASRMFDIPRENHAEIINELMKLPIEFTFFRVGKAG
                     AYAVTRSNAWFCPAVDIAPYVDQTGCGNCSTGAAMYARVAGYEPAMVVTMANIASGYN
                     CAQYGPYPLYTDEVMAQARALSTELVKKVKACAPDRKERMTCR"
     gene            complement(1410..2627)
                     /locus_tag="ACZPFK_08025"
     CDS             complement(1410..2627)
                     /locus_tag="ACZPFK_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406297.1"
                     /translation="MNKKRAIWDSVLISGIHMNYWAGSTIQMTFLVSFMTMSGYTASQ
                     VGLAVAVFSVVNFTCQPVWGYLSDRMKNIRRTIIICLVCATPLLLLLPGAVESRLSYL
                     MIVYTLLACFQGPVAGLLDSLTYGGAETNPYIVYGIARGSGSLFSAVAMLVSGELLYR
                     IGVKYAFVVEAALFVLAAVCAMLYSGTGFESAASPKSERTALGGTIREILQNRACLFL
                     FLSVILLNIGVKAGYTFVPMMVLEMGGTTASNGYVMAINTVGMMPCMLLYNYLHDKRK
                     VSNKILYLLACLFTIARILSLAMVGSMPAMYGIQLISSLQYGFLQPAMLTAITEIVPQ
                     RIRTTAITLAAALQLGVSSLAGDYAAGLLAERIGMRPMFLLLAGVAVLGLASFWHSAR
                     LLERKDNVKSVSG"
     gene            complement(2641..>3165)
                     /locus_tag="ACZPFK_08030"
     CDS             complement(2641..>3165)
                     /locus_tag="ACZPFK_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1406298.1"
                     /translation="VITYLALITIVLVWLFIYKTPLGLRIRTVGENPDAASSVGENPN
                     RIKVIALLIAGFVSSWGGMFLSMGYVSWFTRNMTNARGFIGVAANAVGQGNPLWVALT
                     TFVFAISDALTVVLQSLMVPTEFTSMLPYVLTLTIVVLMALKYQNKQKSLRKKAIEAI
                     LQERKAVAERVGKA"
BASE COUNT          668 a         1014 c          819 g          664 t
ORIGIN      
        1 tccttgttga cgtgtcccac gatgaaaacc gggatatcgg ccgcttttgc cgcccgcatc
       61 atcgcctctg tgacgcgcgc cccaccaccg gaaacgctga tgaccatgac gagcgttttt
      121 tccggcgccc gctgaggaaa tacaaagtgg cggctcacgt cgatcgtgcg cagggcgtga
      181 taatcacagc cggcaaacat ccgttcattc agttcctttg tcgccagggt cgccgcgtag
      241 gaatcgccgc agccggtgac caccacccgt tccagccctt gcaccagctc gtccggcacg
      301 gcctctgaaa gctttttttc gctgagagtt tccagcagcg cctcggcgat cccgggcatg
      361 ctttcaaacc ctattttcat gttgttgtcg cgccctgtca cctgcatgtc atcctctcct
      421 ttctgtccgg ggcacaggcc ttcactttct tcaccaattc ggtggaaagt gcccgggcct
      481 gcgccatcac ctcatccgtg taaagcggat aggggccgta ttgcgcacaa ttatagccgg
      541 aggcgatgtt ggccatcgtc acgaccattg cgggctcata acccgccact ctggcataca
      601 tcgccgcgcc tgtggaacag ttgccgcacc ccgtctggtc gacatagggc gcaatgtcca
      661 cagccgggca gaaccaggcg ttgcttcttg taaccgcata agctcccgct ttgcccaccc
      721 tgaaaaaggt gaattcgatg ggcagcttca tcagctcgtt gatgatctcg gcgtggttct
      781 cccgcgggat atcgaacatc cgggaggcct cattgtagtt caaggaccac atgtcggcaa
      841 tgggcattac ctccttgacc cgttcgaggt cgcgcccgga gttaaaatac tccagctccc
      901 acatgatctg gaacccatat tttattttca ggccctccaa ctttttccag atcacccggt
      961 ccgccccctg cgccatatag atgcccttga cgtcccgggt ggccgcgtcg tcgatcctgt
     1021 cgggatgggt tttcagatac cccagattct cttctccata ataggagcgg gccccccggc
     1081 ctgagccgtc ttcattgtag gacagctcgt agcaggaggt gtgctccgct tccacccgca
     1141 ccgattcctg cgaaaccccg ttgttcctca tccaggcccc ataagtatcg tcgaaatcgg
     1201 cgcccacact gctgaccagc ttgcagctct tggtccacaa gcggatcccc gaaagggcaa
     1261 aaaacgccgg cccccccatg tgcctctcgc tgtggaagcc gtccggctgg accacttcgt
     1321 ccgacatcac gttgccgcat gcaagatatt cgatcattgt ctcatctcct gttctgccgg
     1381 ccgtcccggc ttgttctcgt tcttgtccct taacccgaga cagattttac gttgtctttt
     1441 ctctccagca gacgggctga atgccaaaag ctggccagcc ctagcaccgc cacccccgca
     1501 aggagaagaa acatcggcct catgccgatc cgctcggcca aaaggccggc tgcgtagtcc
     1561 cccgccaggc tgctgacccc cagctgaagc gcggccgcca gcgtgatcgc cgtggtgcgg
     1621 atccgctgcg ggacgatctc ggtgatcgca gtcagcatcg ccggctgtaa aaaaccatac
     1681 tgcagggagc tgatcagctg aatgccgtac atcgccggca tcgaccccac cattgccagg
     1741 ctcaaaatcc gggcaatggt aaacaggcaa gccaacagat ataaaatttt gttggacact
     1801 ttccttttgt catgcaggta attgtatagt agcatacacg gcatcatgcc caccgtattg
     1861 atcgccatga catatccgtt gctggcggtc gtaccgccca tctccagcac catcatcggc
     1921 acaaaggtat atccggcctt gacgccgata ttcagcagga tcacactgag aaagaggaac
     1981 aggcaggccc gattctgcag gatttcccgg atcgtgcccc ccagtgccgt cctctcactt
     2041 ttgggagaag ccgcgctctc aaagccggtt ccgctgtaaa gcatggcgca caccgccgcc
     2101 agaacgaaaa gcgcggcttc caccacaaag gcgtatttta cgccgatccg atagagcagc
     2161 tcgccggaca ccagcattgc cactgcgctg aaaagcgaac cgctgccgcg ggcgatgccg
     2221 tagacgatat agggatttgt ctctgcgcct ccataggtca gcgaatccag caggccggcg
     2281 accggcccct gaaaacaggc cagcagcgta tagacgatca tgagatagct caggcggctc
     2341 tccaccgccc cgggcagcag cagcaacagc ggcgtcgcac acacaaggca gatgataatc
     2401 gtcctgcgga tgtttttcat ccgatctgaa aggtatcccc acaccggctg gcaggtaaaa
     2461 ttgaccaccg aaaacaccgc caccgccagc cccacctgcg aggcggtgta tccgctcatt
     2521 gtcataaacg agacgagaaa ggtcatttgg atcgtggaac ccgcccagta attcatatgg
     2581 atgccggaga tcagcacgct gtcccatatc gctctttttt tgttcatgca gccctcctgc
     2641 tcacgctttg ccaacccgct ctgccacagc ctttcgctcc tgcaggatgg cctcgatggc
     2701 tttcttgcgc aggctctttt gcttgttctg atacttgagg gccatcagga ccacaatggt
     2761 cagcgtcagc acgtagggca gcatagaggt gaactccgtc ggcaccatca aagactgcag
     2821 cactacggtg agagcgtcgg agatcgcaaa aacgaacgtc gtcagagcca cccacagcgg
     2881 atttccctgt ccaaccgcat ttgccgccac gccgataaag cctctggcat tggtcatatt
     2941 gcgtgtgaac caggacacat atcccatcga gaggaacatg ccgccccaag acgagacaaa
     3001 gcccgcgatc agaagggcga tgaccttgat gcggttgggg ttttcgccca ccgagctggc
     3061 ggcgtccggg ttttctccca cggtgcggat gcgcagcccc aagggcgtct tgtaaatgaa
     3121 cagccatacc aggacgatgg tgatcagcgc cagataggtg atgac
//
LOCUS       DBMDYA010000303         3157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6888_length_3157_cov_1.459097, whole genome shotgun sequence.
ACCESSION   DBMDYA010000303 DBMDYA010000000
VERSION     DBMDYA010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6888_length_3157_cov_1.459097"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="ACZPFK_08035"
     CDS             <1..1524
                     /locus_tag="ACZPFK_08035"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1406299.1"
                     /translation="GPEQEYFLVDRRLYEQRRDLIYCGRTLFGAKPPKGQELDDHYFG
                     NLKPRVAQFMHDLDQELWRLGVFAKTEHNEVAPAQHELSPIFTTTNIATDHNQLTMEL
                     MQKVAARHGLVCLLHEKPFAGVNGSGKHNNWSLSTDRGVNLLEPGDSPRENAQFLLFL
                     AAVIKGVDEYQDLLRISVASAGNDHRLGANEAPPAIVSMFVGDDLAAVLHAIETGADC
                     GRQDRVEMRIGVHTLPRFTRDNTDRNRTSPFAFTGNKFEFRMVGSAQSIAGCNTVLNT
                     VVAEELSGFADQLEAAGDFEAALNDLVRRTVSAHKRILFNGNNYADAWVEEARERGLL
                     NLKTTADALPYLIKEKNVALFAKNKIFTEAELRSRYEVLLESYCKTLHIEALTMLEMA
                     KKDILPAALRYAGAVADAALKKKQLCPALGCAPEEALVGRLTALADRLCEGAAGLEAK
                     LPAAEACGDSRAQADYYKEMVLPSMQELRAAADELEGLVAKEFWPYPTYGELLFSVR"
     gene            1690..1935
                     /locus_tag="ACZPFK_08040"
     CDS             1690..1935
                     /locus_tag="ACZPFK_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR1406300.1"
                     /translation="MNIYICVGSSCHLKGSYNIINLMKEALEKNGLTDKVNVSAAFCL
                     GRCTDGVTIKVDDEIITGVSPDNFNEIFDKYVLKGNL"
     gene            1936..>3157
                     /locus_tag="ACZPFK_08045"
     CDS             1936..>3157
                     /locus_tag="ACZPFK_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1406301.1"
                     /translation="MSDFLGLKKSNCKNCYKCIRHCPVKSIKFADDQANIVSNECILC
                     GECFVNCPQNAKVIRDDLPQVKELIASGRPVIASVAPSFISEFGATNFSQVKRVLQKL
                     GFTSAEETAIGAGIVKTRYQEMIADGRHPLIISSCCHTINTLIQKYYPGALPYLADVL
                     SPMLAHCQLIKEQNPGAATVFIGPCISKKEEASIYGICDVVLTFEDLESWMAEEHLVI
                     EPDATDESGKRPRFFPIPGGIIASMDTADTGYSYIAIDGVENCKAALKEIEAGTLTNC
                     FVEMNACEGGCINGPATTTRHQRSIAGAVKVRSFAGREEFDVQLKAPLEKKMDYIGVR
                     AQIPGEAAIKEILAKMGKTSPEKELNCGSCGYNTCREKAIAVYQGKADLSMCLPFLKE
                     KAESFSDKIISNTPN"
BASE COUNT          683 a          995 c          935 g          544 t
ORIGIN      
        1 ggcccggaac aggaatattt tcttgtggac cggcggctgt acgagcagcg gcgcgacctg
       61 atctactgcg gccgcaccct gttcggcgca aagcccccca aggggcagga gctggacgac
      121 cactacttcg gcaacctgaa gccgcgggtt gcacagttta tgcatgacct ggaccaggag
      181 ctatggcgtc tgggggtatt tgccaagacc gagcacaacg aggtggcgcc ggcccagcac
      241 gagctgtccc ccatcttcac caccaccaac atcgccaccg accacaacca gctcaccatg
      301 gagctgatgc agaaagtcgc cgcgcgccat ggcctggtgt gcctgctgca cgaaaagcct
      361 ttcgccgggg tcaacggcag cggcaagcac aacaactggt ccctttcaac cgaccgcggc
      421 gtcaacctgc tggagcccgg ggacagcccg cgtgaaaacg ctcagtttct gctgtttctc
      481 gccgcggtga tcaagggggt ggacgaatat caggacctgc tgcgcatctc ggtggcctcg
      541 gcgggcaacg accatcgcct gggcgccaac gaggccccgc ccgccattgt ctccatgttt
      601 gtgggggacg atctggccgc ggtgctgcac gccatcgaga ccggcgccga ctgcggccgg
      661 caggaccggg tggagatgcg catcggggta cacaccctgc cccgcttcac ccgggacaac
      721 accgaccgca accgcacctc cccctttgcc tttaccggca ataaattcga atttcgcatg
      781 gtgggctcgg cgcagtccat cgcgggctgc aacaccgtgc tcaacaccgt tgtcgcggag
      841 gagctgtccg gttttgccga ccagctggag gccgccgggg attttgaggc ggcgctcaac
      901 gacctggtgc gccgcaccgt ctcggcccac aaacggatcc tcttcaacgg caacaattac
      961 gccgacgcat gggtggagga ggcgcgggag cgcggcctgc tgaacctcaa gaccaccgcc
     1021 gacgccctgc cctacctgat caaagaaaaa aacgtcgccc tgtttgccaa aaacaagatc
     1081 ttcaccgagg cggagctgcg ctcgcgctac gaggtgttgc tggagagcta ctgcaaaacc
     1141 ctgcacatcg aggcgctgac catgctggaa atggccaaaa aagacatcct gcccgccgcc
     1201 ctgcgctatg cgggggcggt ggccgacgcg gcgctcaaaa aaaagcagct ctgccccgcc
     1261 ctcggctgtg cccccgagga agcgctggtg ggcaggctca ccgccctggc ggaccgcctt
     1321 tgtgagggcg cggccgggct ggaggccaag ctgccggcag ccgaagcgtg cggcgacagc
     1381 agggcacagg ccgattacta caaggagatg gtcctcccct ccatgcagga gctgcgggcc
     1441 gctgcggacg agctggaggg gcttgtggcg aaagaatttt ggccctatcc cacctacggc
     1501 gagctgctgt tcagcgtgcg ctgagccccg cagcgaaaat gtcttaaatt tcacgcttgc
     1561 agaacgtttt gcgacttgac cctggcattg gttctattat ataataggta tgttaaatag
     1621 ttaacatacc tatttttttt actctttttt gtctaaagtt cccaaaaatc acgaaagaag
     1681 gggtcagtta tgaacattta catctgtgtg gggagctcgt gccatctcaa gggctcttac
     1741 aacatcatca acctgatgaa agaggctctg gaaaaaaacg gcctgaccga caaggtcaac
     1801 gtctcggcgg cgttctgcct gggccgctgc accgacgggg tgaccatcaa ggtggacgac
     1861 gagatcatca ccggcgtttc gcccgacaac ttcaacgaga tctttgacaa atatgtcctg
     1921 aaggggaatc tgtaaatgag cgacttcctg gggctgaaaa agtccaactg caagaactgc
     1981 tataagtgca tccgccactg cccggtcaag agcatcaaat ttgccgacga ccaggcgaac
     2041 atcgtgtcga atgagtgcat tctgtgcggc gaatgctttg tcaactgccc acagaacgcc
     2101 aaggtcatcc gggacgacct gcctcaggtg aaagagctga tcgcctccgg ccggccggtc
     2161 atcgccagcg tggccccctc gttcatctcc gagtttgggg cgaccaactt cagccaggtc
     2221 aagcgggtgc tgcaaaagct gggcttcacc tcggcggagg agaccgccat cggcgccggc
     2281 atcgtcaaga cccgctatca ggagatgatc gccgacgggc ggcatccgct gatcatctcc
     2341 tcctgctgcc acaccatcaa caccctgatc cagaaatact atcccggcgc gctgccctat
     2401 ctcgccgacg tgctctcgcc catgctggcc cactgccagc tgatcaaaga gcagaacccc
     2461 ggcgcggcga cggtctttat cggcccctgc atctccaaaa aagaggaggc cagcatttac
     2521 ggcatctgcg acgtggtgct cacctttgag gacctggaga gctggatggc cgaggagcat
     2581 ctggtcatcg agccggacgc caccgacgag agcggcaagc ggccgcgctt cttccccatc
     2641 ccgggcggca tcatcgccag catggacacg gcggacaccg gctacagcta catcgccatc
     2701 gacggggtcg aaaactgcaa ggcggccctc aaggagatcg aggccggcac gctcaccaac
     2761 tgctttgtgg agatgaacgc ctgcgagggc ggctgcatca acggcccggc caccaccacg
     2821 cggcatcagc gcagcatcgc gggcgcggtg aaagtgcgct cgttcgcggg cagggaggaa
     2881 ttcgacgtcc agctgaaagc cccgctggaa aagaagatgg actacatagg cgtgcgcgcc
     2941 cagatcccgg gcgaggcggc catcaaggag attctggcca agatgggcaa gaccagcccg
     3001 gaaaaagagc tcaactgcgg cagctgcggc tacaacacct gccgcgaaaa ggccattgcc
     3061 gtctaccagg gcaaggccga cctgtcgatg tgcctgccct ttttgaaaga aaaggcggag
     3121 agcttttccg acaagatcat cagcaacacc cccaacg
//
LOCUS       DBMDYA010000304         3151 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6898_length_3151_cov_1.698033, whole genome shotgun sequence.
ACCESSION   DBMDYA010000304 DBMDYA010000000
VERSION     DBMDYA010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6898_length_3151_cov_1.698033"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(48..485)
                     /locus_tag="ACZPFK_08050"
     CDS             complement(48..485)
                     /locus_tag="ACZPFK_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR1406302.1"
                     /translation="MKLKIEQSPACQEVEITVRCAQMDARLAALLEQIRLYGFSVAGL
                     RDGRSYQVPLDEVLYIEAVEEKTFLYRSGDVLECRSRLYELEEQLRGTSFVRVSRNCL
                     LNLRRLESVSPQFDGRFEARMENGETVIVNRHYVKSFKQRFGL"
     gene            complement(688..1380)
                     /locus_tag="ACZPFK_08055"
     CDS             complement(688..1380)
                     /locus_tag="ACZPFK_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1406303.1"
                     /translation="MAEVLSNPVFGIALSVMAYAVGVWLNKKLKTPLANPLLIAVVLL
                     IAFFLLTGIPMEYYDEGGDIINFLIFPATASIGLSIYANFDILKKNLVPVLGGCLVGA
                     VTSVASVWGLCRLFSLDATLTASLLPKSVTTAIAVPISEQNGGIPQITIVALMIAGTL
                     GAVLAPSLSKWFHLEDNEVATGLATGCASHAAGTSTAVKMSETVGAMSGLAIGVCGVF
                     TVILAIFFKYLL"
     gene            complement(1373..1756)
                     /locus_tag="ACZPFK_08060"
     CDS             complement(1373..1756)
                     /locus_tag="ACZPFK_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015729.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1406304.1"
                     /translation="MNILWQVGILFLVCLAAQGLVTLLPFAFPASVLAMILVFLLLLA
                     GIIKVRQIKELSNWFAQNMAFLFVPACAGVLNYFDIIAANLFPILFISTVTTFLVFGA
                     TGWTVKGLMRLQARLREGKGERDDG"
     gene            complement(2020..>3151)
                     /locus_tag="ACZPFK_08065"
     CDS             complement(2020..>3151)
                     /locus_tag="ACZPFK_08065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1406305.1"
                     /translation="GGFSDPLMDCKDCKTRHRADKLIEDAGGDANGMTFEQMSAYIKE
                     HGITCPNCGSANFTDIRKFNLMFKTFQGVTEDAKSELYLRPETAQGIFVNFANVQRST
                     RRKLPFGICQIGKTFRNEITPGNFTFRTREFEQMEMEFFCKPDTDLDWFYYWKDYCKK
                     FLLGLGMREENLRLRDHEKEELSFYSKATTDIEYLFPFGWGELWGIADRTDYDLGRHN
                     EVSGKVQDYFDPETNEHYVPYVVEPSLGADRVALAFLVEAYDEEVVDAQKNDTRVVLR
                     LHPALAPYKACVLPLSKKLNDKAGEVYAALQKHFMVDYDDAGSIGKRYRRQDEIGTPI
                     CVTYDFDSESDGCVTVRDRDTMQQERIKIEELVSYIEGKIAY"
BASE COUNT          576 a         1035 c          938 g          602 t
ORIGIN      
        1 caggtggtca ccgcgtcccg gattctctct ttcatctcaa gccctcctca aagcccgaag
       61 cgctgtttaa agctcttcac atagtggcgg ttgacgatca ccgtctcgcc gttttccatc
      121 ctcgcctcaa acctgccgtc aaactgaggc gaaacgctct ccagccggcg caggttcaac
      181 aggcagttgc ggctgacccg cacaaaactg gtgccgcgca gctgctcctc cagctcgtaa
      241 aggcggctgc ggcattccag cacatcgccg ctgcggtaga gaaaggtctt ctcctccacc
      301 gcctcgatat agagcacctc gtccagcggc acctgatagc tgcggccgtc ccgcaggccc
      361 gccaccgaaa acccgtacag ccgtatctgc tccagcagag cggccagccg cgcgtccatc
      421 tgcgcgcagc gcaccgtgat ctccacctcc tggcaggcgg gcgactgttc gatcttgagc
      481 ttcaacgccg ttccccctct cccctgtatc cgcccgtgcg ggctgccggc ccgcgccggt
      541 ccatatctcc attgtacccg tttcccccgc cctgcaaaag gaagcccgcc cccctttttc
      601 tgcactttgc cgcccccggc ggcaggcggc ataaaaagag caggcccggc tggttccagc
      661 cgggcccgcg ctcacatttt ttgagggtca gagcagatat ttgaaaaaga tggccaggat
      721 cacggtgaaa acgccgcaca ccccgatggc aaggccgctc atggcgccca ccgtctcgct
      781 catcttcacc gccgtggagg tgcccgccgc gtggctggca cagccggtgg cgaggccggt
      841 cgccacctcg ttgtcctcca gatgaaacca cttggacagg ctgggggcca gcaccgcccc
      901 cagggtgccc gcgatcatca gcgccacgat ggtgatctgc gggataccgc cgttctgctc
      961 gctgatgggc accgcgatgg cggtggtcac cgatttgggc agcaggctgg cggtgagggt
     1021 ggcgtccagg gagaacagcc tgcacaggcc ccatacgctg gccacgctgg tcaccgcccc
     1081 caccaggcag ccgcccagca cggggacgag gttctttttc aggatatcga agttggcata
     1141 gatcgacagg ccgatggagg cggtggccgg gaagatcagg aaattgatga tgtccccgcc
     1201 ctcgtcatag tactccatcg ggatgccggt caggaggaaa aaggcgatca gcagcaccac
     1261 cgcgatgagc agcgggtttg ccagcggggt cttgagcttt ttgttcagcc agacccccac
     1321 cgcataggcc atcaccgaca gggcgatgcc gaacaccggg tttgagagca cctcagccat
     1381 cgtcccgttc cccctttccc tctctcagcc gcgcctgcag ccgcatcagc ccctttaccg
     1441 tccagccggt cgccccaaag acgagaaagg tggtgacggt gctgatgaag aggatgggga
     1501 acaggttggc cgcgatgatg tcgaaatagt tgagcacccc cgcgcaggcg ggcacaaata
     1561 aaaaggccat gttctgcgca aaccagttgg acagctcttt gatctgccgc accttgatga
     1621 tgccggccag cagcagcaaa aacaccaaaa tcatcgccag cacgctcgcc ggaaaggcga
     1681 acggcagcag ggtcaccagc ccctgggccg caaggcagac cagaaacagg atgcccactt
     1741 gccacaagat attcaaaatt ttccctccta cccggcgggt ccgggggcgc gcaccccgcg
     1801 ccgcccccgc cgccctgtgg cgcacagccg ccttgccccg ggggcacgct tttctgcaca
     1861 cagatgttat tatagcagac tgccgcgcgc tttcccactg gcagaagcaa aaaaacagcg
     1921 gctctcaggc cgctgttttt gggcgtattg tgggtgcggt tgccgttttt cccgccctgg
     1981 atgaagcggg gcgggccgct ttgccgcggc tgaccacggt caataggcga tcttgccctc
     2041 gatgtaggac acgagctcct cgattttgat gcgctcctgc tgcatggtgt cgcggtcgcg
     2101 cacggtgaca cagccgtcgc tctccgagtc aaagtcgtag gtcacgcaga tcggcgtgcc
     2161 gatctcgtcc tggcggcggt accgcttgcc gatggacccg gcgtcgtcgt aatccaccat
     2221 gaaatgcttt tgcagcgcgg cgtagacctc gcccgccttg tcgttcagct ttttggacag
     2281 gggcagcacg caggccttgt agggcgccag ggccggatgc aggcgcagca ccacgcgggt
     2341 gtcgttcttc tgcgcgtcca cgacctcctc gtcgtaggcc tccaccagaa aagccagcgc
     2401 cacgcggtcg gcgcccagcg agggctccac cacatagggc acatagtgct cgtttgtctc
     2461 ggggtcaaag tagtcctgca ccttgcccga cacctcgttg tgccggccca gatcgtaatc
     2521 ggtgcggtcc gcgatgcccc acagctcgcc ccacccgaac gggaacaggt actcgatgtc
     2581 ggtggtggct ttcgaataga aagagagctc ttccttttcg tggtcgcgca ggcgcaggtt
     2641 ctcctcccgc atgcccagcc ccagcaggaa ttttttgcag tagtctttcc agtaatagaa
     2701 ccagtcgagg tcggtgtccg gcttgcagaa gaactccatc tccatctgct cgaactcgcg
     2761 ggtgcggaaa gtgaagttgc ccggggtgat ctcattgcgg aaagttttgc cgatctggca
     2821 gatgccgaac ggcagcttgc gccgggtgga gcgctgcacg ttggcaaagt tgacaaagat
     2881 gccctgcgcg gtctcggggc gcaggtagag ctcgctcttt gcgtcctcgg tgacgccctg
     2941 gaacgtcttg aacatcaggt tgaacttgcg gatgtccgta aagttggcgc tgccgcaatt
     3001 ggggcaggtg atgccgtgct ctttgatgta agcgctcatc tgctcaaagg tcatgccgtt
     3061 ggcgtcgccg cccgcgtcct cgatcagctt gtcggcgcgg tggcgggtct tgcagtcctt
     3121 gcagtccatc agcgggtcgg agaagccgcc c
//
LOCUS       DBMDYA010000305         3151 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6899_length_3151_cov_1.480656, whole genome shotgun sequence.
ACCESSION   DBMDYA010000305 DBMDYA010000000
VERSION     DBMDYA010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_3151_cov_1.480656"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="ACZPFK_08070"
     CDS             <1..333
                     /locus_tag="ACZPFK_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406306.1"
                     /translation="FSFGAFSLGLLLSIGAISVGCISLGALAVGVVALGACALGVYAA
                     GAAAVASSVAVGAAASAPVAVGVEARGLHALSGADQLTREEILAFLAQWAPGLWKPLR
                     DLLTLFLS"
     gene            complement(632..1087)
                     /locus_tag="ACZPFK_08075"
     CDS             complement(632..1087)
                     /locus_tag="ACZPFK_08075"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR1406307.1"
                     /translation="MVVSVGSDHAGFGLKMKMLEVIRGWGYEVVDNGCYDNSAPVYFP
                     EVAKKVCQPVLDGKAEKAIMFCGTGVGASIACNKIPGIRASIIHDVHCAHQAVEHDDV
                     QVMCIGEKIVGEWLAPDLIRVFLEARANQDDWTPHVVRMLNEMDGSSEG"
     gene            complement(1105..1845)
                     /locus_tag="ACZPFK_08080"
     CDS             complement(1105..1845)
                     /locus_tag="ACZPFK_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406308.1"
                     /translation="MLKIKDLVSRYGSIEALHGISLEVGPSEMVALIGANGAGKTTLL
                     NSISGTVTASGSIAFAGNEILQKKAAAIARMGLLQVPEGRHVFPGLTVEENLLVGTVA
                     SRGMRLRPGDNSQEMEMVFETFPRLKERRRQLAWSMSGGEQQMLAIGRALMGRPKLLM
                     LDEPSMGLAPIVIDELFEKIVEINRTGVPILMVEQNARLALRVSDRAYILERGAITSS
                     GPSRQLLQDPRVLEAYLGKSVARAAEND"
     gene            complement(1832..2605)
                     /locus_tag="ACZPFK_08085"
     CDS             complement(1832..2605)
                     /locus_tag="ACZPFK_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406309.1"
                     /translation="MNACRSAEKSPKLVLSEVCKNFGGVTAAQDICLEIYGGEVLGLI
                     GPNGAGKTTLINLITGVYPVTRGAVTFGGRDITRQPTHKRAIEGIGRTFQHPHLLDRC
                     DVLTNIIMGSDMAARKRGAGRADLKRELPELLRCAGLENIDLTDPVSKLAYGQQKLLE
                     VVRVLLSRPEVLLLDEPAAGLNNRETQYVADLIRHATRQGIGVLLIEHSMELVMSICD
                     RITVLNFGTQIATGTPAEIQNNEEVIRAYLGGGGSDAED"
     gene            complement(2556..>3151)
                     /locus_tag="ACZPFK_08090"
     CDS             complement(2556..>3151)
                     /locus_tag="ACZPFK_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1406310.1"
                     /translation="FMNWEAFSGGAVGLKNIPTFSLFGWKATSEQSKFYIVLVVCLAA
                     TALIQLLFHSALGRSFMAIRDNEMTADSMGINSLLTKSIAFAISGALCGAAGALHASL
                     SGYLSYQSFTYNQSTIYLIMIMIGGTVSPVGAVIGTCVITILQEGFRALQDYMQLIYG
                     VGVIVLMIFQPEGILGGGKALYERLSERRKKSKTGAV"
BASE COUNT          483 a         1057 c         1014 g          597 t
ORIGIN      
        1 ttcagctttg gggctttctc gctgggcctg ctgctctcga tcggcgccat ctcggtgggc
       61 tgcatttccc tgggcgcgct ggcggtgggg gtggtggcgc tgggggcctg cgccctcggc
      121 gtctacgcgg cgggcgcggc ggcggtggcc tcctccgtcg ccgtcggcgc cgcggccagc
      181 gcgccggttg ccgtgggggt ggaggcccgc ggcctgcacg ccctgtcggg tgcggaccag
      241 ctcacgcggg aggagattct cgcctttctg gcccagtggg cccccggtct ctggaagccg
      301 ctgcgggacc tgttgaccct gtttttgtcc tgaagagcgt ctgttgaaag gtatgcccgg
      361 ttgttttctc cgcgacacgt caaagagcac atgagccgcc gcaaaggacg ggctcatgtg
      421 ctctttcttt ttgtgttttc tctttggcgg cgcagcgccc gccagcgctg aaagcgccgc
      481 agaggcaggg ggcagggcct gcggggcgga tacgggcggc ttggccccgg cgggcccggc
      541 ccgcagcaca gcgccatggc cggggatgtg agcctgcttc accgggcgga acacacccgg
      601 ccccaggccg ccctgcggcg cagcgcggct ctcagccctc gctgctgccg tccatctcgt
      661 tcagcatgcg caccacgtga ggggtccagt cgtcctggtt ggcgcgcgcc tcgagaaaga
      721 cgcggatgag gtcgggggcc agccactccc ccacgatctt ctcgccgata cacatcacct
      781 gcacgtcgtc gtgctccacc gcctggtggg cgcaatgcac gtcatggatt atggacgccc
      841 ggatgccggg gatcttgttg caggcgatgg aggcgcccac cccggtgccg cagaacatga
      901 tcgctttttc cgctttgccg tccagcaccg gctggcagac cttttttgcc acctcgggga
      961 aatacaccgg cgcgctgttg tcatagcagc cgttgtccac cacctcatat ccccatccgc
     1021 ggatgacctc gagcatcttc atcttcaggc cgaacccggc gtggtcgctt cccactgata
     1081 caaccatcga atccactcct tttatcagtc gttttccgcc gcccgcgcga cggattttcc
     1141 caaatacgcc tccagcaccc gcgggtcctg cagcagctgg cgcgaggggc cgctggaggt
     1201 gatggcgccg cgctccagga tataggcgcg gtcggacacc cgcagcgcca gccgcgcgtt
     1261 ctgctccacc atcaggatgg gcacgccggt gcggttgatc tccacgatct tttcaaacag
     1321 ctcgtcgatc acgatgggcg caaggcccat gctgggctcg tcgagcatca gcagcttggg
     1381 ccgccccatc agcgcgcggc cgatggccag catctgctgc tcgccgccgc tcatgctcca
     1441 ggccagctgc ctgcgccgct ctttcaggcg cggaaaggtc tcaaagacca tctccatctc
     1501 ctgcgagttg tcgcccgggc gcagccgcat gccgcggctg gccaccgtgc ccaccagcag
     1561 gttttcctcc accgtcagcc cggggaagac gtgccgaccc tccggcacct gcagcagccc
     1621 catgcgcgcg atggcggcgg cctttttctg caatatctca ttgcccgcaa aggcgatgct
     1681 gccgctggcc gtcacggtgc cggagatgct gttgagcagc gtggtcttgc ccgcgccgtt
     1741 ggcgccgatg agggccacca tctcgctggg ccccacctcc aggctgatgc cgtgcaaagc
     1801 ctcgatgctg ccgtagcgcg agaccaggtc tttaatcttc agcatcgctg ccgcctcctc
     1861 ccagataggc ccggatgacc tcttcgttgt tctggatctc cgccggtgtg ccggtggcga
     1921 tctgggtgcc gaaattcagc accgtgatgc ggtcgcagat gctcatgacc agctccatcg
     1981 agtgctcgat gagcagcacg ccgatgccct gccgggtggc gtggcggatc aggtcggcca
     2041 cgtactgtgt ctcccggttg ttgaggccgg cggccggctc atccagcagc agcacctcgg
     2101 gccggctgag cagcacccgc accacctcca gcagcttctg ctgcccatag gccagcttgc
     2161 tcaccgggtc ggtcaggtcg atgttttcaa gaccggcgca gcggagcagc tccggcagct
     2221 cccttttcag gtccgccctg cccgcgccgc gcttgcgggc ggccatatcg ctgcccatga
     2281 tgatgttggt gagcacgtcg cagcggtcca gcaggtgcgg gtgctgaaag gtgcgcccga
     2341 tcccctcgat ggcccgcttg tgggtgggct ggcgggtgat gtcccggccg ccaaaggtga
     2401 cggcgccgcg ggtgaccgga tacacgccgg tgatcaggtt gatgagggtg gtcttgcccg
     2461 cgccgttggg cccgatcagg cccagcacct caccgccgta gatctccagg cagatgtcct
     2521 gcgccgcggt gacgccgcca aaatttttgc agacctcaga cagcaccagt tttggacttt
     2581 tttctgcgct ccgacaggcg ttcatacagc gctttccccc ctcccaaaat tccttccggc
     2641 tggaagatca tcagcacgat gacccccacg ccgtagatga gctgcatgta gtcctgcagg
     2701 gcgcgaaagc cctcttgcag gatggtgatc acgcaggtgc cgatcaccgc gcccaccggc
     2761 gagacggtgc cgccgatcat gatcatgatc agatagatgg tggactggtt gtaggtgaag
     2821 ctctggtagc tgagatagcc ggacagcgag gcgtgcagcg cgcccgccgc gccgcagagc
     2881 gcgccggaga tggcaaaggc gatgctcttg gtgagcaggc tgttgatgcc catgctgtcg
     2941 gccgtcatct cattgtcgcg gatggccata aaagagcgac ccagcgcgga gtgaaagagc
     3001 agctggatca gcgccgtcgc cgccaggcag acgacgagca cgatgtagaa tttgctctgc
     3061 tcgctggtgg ccttccagcc gaagagggaa aaggtgggga tattcttcag gcccaccgcg
     3121 ccgccggaaa aggcctccca gttcatgaac a
//
LOCUS       DBMDYA010000306         3147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6911_length_3147_cov_1.204531, whole genome shotgun sequence.
ACCESSION   DBMDYA010000306 DBMDYA010000000
VERSION     DBMDYA010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_3147_cov_1.204531"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="ACZPFK_08095"
     CDS             <1..233
                     /locus_tag="ACZPFK_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406311.1"
                     /translation="SLIRKTIILYFPAFVKERSPQVSTREGGYVTRREDFCQRKREEG
                     ERTARMVEFEGVNVERKEASAGSGRLFVEKRG"
     gene            complement(230..1534)
                     /locus_tag="ACZPFK_08100"
     CDS             complement(230..1534)
                     /locus_tag="ACZPFK_08100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR1406312.1"
                     /translation="MEQFDLIVIGAGPGGYTAALEAAALGKRVALCEKDEVGGTCLNR
                     GCVPTKALLHGAGPGCDWPALAAEVERVVSTLRAGQEKQLRAAGVELIPYRAVVTGPH
                     TVEAGGAAYEAAHILVAAGSEPALPPIPGLDTPGVVTSDGLLSDLRPLDRLAIIGGGV
                     IGVELACVWAAAGARVTILEAAPRLLPTLERELGQSLAAQLRQDGVEVFTGAGVTGAT
                     PGPAVAFTHRGEAYRLEADAVLVATGRRPATGGLFAGDLLPELERGFLRVDGQFRTSI
                     PSLYAIGDAIGGAMLAHKAEAEGRFVADLLLGRTPLRGVAPIPACVYTSPELAQVGLT
                     ADEARARGIPCAAGKCVLGGNARTLIEGGKRGFVKLVFHRESRALLGAQLCCYRATDL
                     ISELALAVTLELTAEQLLRPVRPHPTFAEAISEAVEAAFPLS"
     gene            complement(1521..2531)
                     /locus_tag="ACZPFK_08105"
     CDS             complement(1521..2531)
                     /locus_tag="ACZPFK_08105"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIR1406313.1"
                     /translation="MSCEPLYLVTDCTDPVRNLALEAALLEAVQPGQCLLYLWQNRNT
                     VVIGRNQNSRRECRLDLLEADGGRLVRRMSGGGAVYHDLGNLNFTFLACEGVYDLDRQ
                     LEVVLRAVRGLGVPAEKTGRNDLTADGRKFSGNAFYRAGDRRYHHGTLLLSADGEKMA
                     RYLSVAPEKLRSKGVASVRARVCNLAEYVPGLTARRLVQALLDAFGEVYGSEPELFAY
                     PLDAARLRALEEKFASDEWRFGREPRADCTLSGRWPWGGAELCLTVAGGRVTAATVYT
                     DAMDAGLAAALEAALTGSLFAAQPLAAAARALAGRENDRGERRAMALDIAALLEEMDG
                     TI"
     gene            complement(2521..>3147)
                     /locus_tag="ACZPFK_08110"
     CDS             complement(2521..>3147)
                     /locus_tag="ACZPFK_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR1406314.1"
                     /translation="PGDMGFDVVHLNLHKTFCAPAPHVAQGGRSYVFETPPKTIGTVK
                     AFYGNFLVVVRALAYLLTLGGAGLREASENAVLNANYLKTLLEPYAPPACAGVCMHEF
                     VVTLEQLKKDTGVTALDVAKAMLDCGIHPPTMYFPLIVHEALMFEPTETESKETLDWA
                     AGEIIEILKRAPADPDSLHAAPHGTPVGRPDEVAAARTPRLRYRFDEL"
BASE COUNT          519 a         1093 c         1067 g          468 t
ORIGIN      
        1 cttctttgat ccgtaagacc attatattgt attttccagc atttgtaaag gagcgatcac
       61 cgcaggtttc gacacgggag ggcggttatg tcacccgcag agaggacttc tgtcaacgaa
      121 agcgcgaaga aggggagaga acggcgagaa tggtcgaatt tgagggagtg aatgtggaaa
      181 gaaaagaggc gtccgccggc agcggacgcc tctttgtgga aaaaaggggc taggagaggg
      241 gaaaagccgc ctccacggcc tcggaaatgg cctcggcaaa ggtgggatgg gggcgcaccg
      301 ggcggagcag ctgctccgcc gtcagctcca gcgtgaccgc caaggcgagc tcggagatca
      361 gatcggtggc ccggtaacag cacaactgag cccccagcag ggcgcggctc tcccggtgaa
      421 ataccagctt gacaaagccc cgctttcccc cctcgatgag ggtacgggcg ttgcccccca
      481 gcacacactt gccggcggca caggggatgc cccgggccct ggcctcgtcg gcggtgagtc
      541 ccacctgggc cagctctggg ctggtgtaga cgcaggcggg gatgggggcc actccgcgga
      601 ggggggtgcg gcccagcagg aggtcggcca caaagcgccc ctccgcctcc gccttgtggg
      661 ccagcatggc cccgccgatg gcgtccccga tggcgtaaag ggaggggatg gaggtgcgga
      721 actgtccgtc cacccggagg aacccccgct ccagctccgg caggaggtcc ccggcaaaga
      781 ggccgccggt ggcgggccgc cgcccggtgg ccaccagaac ggcgtcggcc tccagccggt
      841 acgcctcccc ccggtgggtg aaggcgacgg cagggccggg ggtggcgccg gtgacgccgg
      901 ctccggtgaa cacctccacc ccgtcctgcc tgagctgcgc ggccagactc tggcccagct
      961 cccgctccag ggtgggcagg aggcgggggg ccgcctccag aatggtgacg cgggcgcctg
     1021 cggcggccca gacacacgcc agctccaccc cgatcactcc gccgccgatg atggccagac
     1081 ggtccagggg acgcagatcg gacagcaggc cgtcgctggt gactacaccg ggtgtgtcca
     1141 ggccggggat ggggggcagg gcgggctcgg agcccgcggc gaccaggatg tgggccgcct
     1201 cgtaggccgc gccccccgcc tccaccgtgt gggggccggt gaccaccgcc cggtaaggga
     1261 tgagctccac cccggcggcc cggagctgct tctcctggcc ggcgcggagg gtggacacca
     1321 cccgctccac ctctgcggcc agggcgggcc agtcacagcc aggccccgcc ccgtggagga
     1381 gggccttggt ggggacgcac ccccggttga ggcaggtgcc ccccacctcg tccttttcgc
     1441 agagtgccac ccgttttccc agggcggccg cctcgagagc ggcggtatat ccgcccggcc
     1501 ccgcgccgat cacgatcaga tcaaattgtt ccatccattt cctccagcag cgcggcgata
     1561 tccagcgcca tcgcgcggcg ttccccgcgg tcgttttccc gcccggccag cgcccgcgcc
     1621 gcggcggcca ggggctgcgc ggcaaagagg ctgccggtga gggcggcctc caaggccgcc
     1681 gccagcccgg cgtccatggc gtcggtgtag acggtcgccg ccgtcacccg gccccctgcg
     1741 acggtgaggc agagctccgc cccgccccag ggccagcggc cggacagggt gcaatccgcc
     1801 ctcggctcgc gaccaaaacg ccactcgtcc gatgcaaact tttcttccag cgcgcgcagc
     1861 cgcgccgcgt ccagcgggta ggcaaacagc tcgggttcgc ttccatagac ctcgccaaag
     1921 gcgtccagca gcgcctgcac gagcctcctt gccgtcaggc cggggacata ctccgccaaa
     1981 ttgcagaccc gcgcgcgcac tgaagccacc cctttcgagc gcagcttttc aggtgccacg
     2041 ctgagatagc gggccatctt ttcgccgtcc gccgacagca gcagcgtgcc gtgatgatag
     2101 cgcctgtccc cggcccggta aaaggcattg cccgaaaact tgcgcccatc ggccgtcagg
     2161 tcgttgcgcc cggtcttttc cgccgggacg cccaggcccc gcacggcgcg cagcaccact
     2221 tccagctggc gatccaggtc gtaaacgccc tcacaggcga gaaaggtgaa gttgaggttg
     2281 cccaggtcgt ggtagacggc gccgccgccc gacatgcgcc gcaccagcct gccgccgtcc
     2341 gcctcgagca ggtccaggcg gcactccctg cggctgtttt ggttgcggcc gatcaccacg
     2401 gtgtttcggt tttgccacag gtagagcagg cattgccccg gctgcaccgc ctccagcaga
     2461 gcagcctcca gcgccaggtt gcgcaccggg tcggtacaat ctgtgacaag gtaaagaggc
     2521 tcacagctca tcaaaccggt acctcagcct gggggtgcgc gccgcagcca cctcgtccgg
     2581 ccgtcccacc ggggtgccgt gaggcgcggc gtgcagcgaa tcgggatcgg ccggggcgcg
     2641 tttgagtatt tcgatgatct cccccgccgc ccagtccagc gtctctttgg attcggtctc
     2701 ggtgggctcg aacatcagcg cctcgtgcac gatgagcggg aaatacatcg tcggcgggtg
     2761 gatgccgcag tccagcatgg ccttggccac gtccagcgct gtgacgccgg tgtccttttt
     2821 aagctgttcc agcgtcacca cgaactcgtg catgcagacc cccgcgcagg ccgggggcgc
     2881 atagggctcc agcagcgttt tgaggtagtt ggcgttgagg acggcgttct cagacgcctc
     2941 ccgcaggccc gcgcccccca gcgtgagcag ataggccagc gcccgcacca ccaccaggaa
     3001 gttgccgtaa aaggccttga ccgtgccgat ggtcttgggc ggcgtctcaa agacataact
     3061 ccgcccgccc tgcgccacgt ggggcgcggg ggcgcaaaag gtcttgtgca gattgagatg
     3121 caccacgtca aagcccatgt cgccggg
//
LOCUS       DBMDYA010000307         3144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6919_length_3144_cov_1.673349, whole genome shotgun sequence.
ACCESSION   DBMDYA010000307 DBMDYA010000000
VERSION     DBMDYA010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6919_length_3144_cov_1.673349"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="ACZPFK_08115"
     CDS             <1..309
                     /locus_tag="ACZPFK_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013813.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406315.1"
                     /translation="HPAFPDGTNVDFVQVVGRDRLRIATWERGAGHTLACGTGCCAAA
                     VAARTSGRITGDRVRLETEGGPLQVGFLPDGSVRLIGGAETVCTGVLSANLLCRRPRA
                     "
     gene            570..1946
                     /locus_tag="ACZPFK_08120"
     CDS             570..1946
                     /locus_tag="ACZPFK_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1406316.1"
                     /translation="MNKAKRLVALALALTLAAGAFSGCWNKDDSSSSGGSGSASSGSG
                     KVYFLNFKPEVDSVWQTIAKEYTQKTGVQVKVTTAASGTYEEQLKSEMAKKEAPTIFQ
                     VNGPVGYASWADWCADISGSKLAGRLREGAGVLGDGEKIYGIPYTVEGYGIIVNTALM
                     DRYFALEGAKAKSLEEIDGFEKLKAVVEDMQAKKAELGIDGVFASTSLLSGEDWRWQT
                     HLANVALAPEFDELGADMTDAKTAPEITFKYAENFKNLFDLYLDNSTIDAKLSGSKTV
                     NDSMAEFALGKCAMVQNGNWGYSQIAGVEGNVVQEGDVAFLPMYMGLPGEESQGIAIG
                     TENFLCINAQASEADRKASLDFLDWLFSSEEGKAHVKGELGFIAPFDTFTAEDAPADP
                     LGRAVVEWQNRDGVKNTPWDFTIFPSQTFKDNFGSALLRYAQGSLAWDELVRSVVADW
                     KTEKANIA"
     gene            2176..3030
                     /locus_tag="ACZPFK_08125"
     CDS             2176..3030
                     /locus_tag="ACZPFK_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1406317.1"
                     /translation="MQKTLKKARWFTVFVVPTLLAFLASFLAPFLMGIYLSFCKFTTV
                     TNARWVGLDNYLRVFTDPGGDFAHALGFTALFTVVSVVTVNLFAFALALLLTRGLRGT
                     NLFRTVFFMPNLIGGIVLGYIWQIIINGVLLGFGVDITFDAKYGFWGLVVLMNWQLIG
                     YMMIIYIAGLQNVPPELIEAAEIDGASGWQTLIHVTLPNVMPSVTICTFLTLTNSFKL
                     FDQNLALTGGAPGKQTAMLALDIYNTFYKRTGWEGVGQAKAVLFFLMVGVIAFIQLRL
                     TRSKEVEN"
BASE COUNT          564 a          949 c         1043 g          588 t
ORIGIN      
        1 catccggctt ttcccgacgg caccaatgtg gattttgtgc aggtggtggg cagggaccgg
       61 ctgaggattg ccacctggga gcgcggcgcg ggccacacgc ttgcctgcgg caccggctgc
      121 tgcgcagccg ccgtggcggc gcgcaccagc ggcaggatca cgggcgaccg ggtgcggctg
      181 gagacggagg ggggcccctt gcaggtgggc tttttgccgg acggctcggt gcggctgatc
      241 ggcggcgccg agaccgtctg caccggcgtg ctgtcggcaa acctgctctg ccgccgcccg
      301 cgcgcctgac aggggggcgg gggcaaggcc gctttctttg gctggtgctt ttatggccgc
      361 gcaccggtgc gcatggcgga gagagggcgg tccggcgcgc acgtccgtct gccgcgctgc
      421 gctgggagga cggcttaaaa atgacagctt gatgaaaaag cggcggcgga cggactaaaa
      481 aacttttttt gtcccatcct aacatcgaat ccaagcggag gggcggcggg atcgctgccc
      541 ccggccgcga atgaaatggg aggatggcta tgaacaaggc aaaaaggctc gtggccctgg
      601 cgctggcgct gaccctggcg gcgggcgcgt tttccggctg ctggaacaag gacgactcct
      661 cgtcctccgg cggctccggc tcggcttcga gcgggtcggg caaggtgtat tttttgaact
      721 ttaagcccga ggtggacagc gtctggcaga ccatcgccaa ggagtacacc cagaagaccg
      781 gcgtgcaggt caaggttacc acggcggctt cgggcaccta cgaggaacag ctcaaatcgg
      841 agatggcgaa aaaagaggct cccaccatct ttcaggtcaa cggccctgtg gggtacgcct
      901 cctgggcgga ctggtgcgcc gacatctcgg gcagcaagct ggccgggcgc ctgcgcgagg
      961 gcgccggggt gctgggcgac ggcgaaaaga tctacggcat cccctacacg gtggagggct
     1021 acggcatcat cgtgaacacc gccctcatgg accgctattt cgcgctggag ggcgcaaagg
     1081 caaagtcgct ggaggagatc gacggctttg aaaagctgaa agcggtcgtc gaggacatgc
     1141 aggccaaaaa ggccgagctg ggcatcgacg gcgtgttcgc ctccacctcg ctgctctccg
     1201 gcgaggactg gcgctggcag acccatctgg ccaacgtggc gcttgcgccg gagttcgacg
     1261 agctgggcgc ggatatgacc gacgccaaga ccgcgccgga gatcacgttc aagtacgccg
     1321 aaaactttaa aaacctgttc gacctctatc tggacaactc caccatcgat gcaaagctgt
     1381 cgggcagcaa gacggtgaac gactccatgg cggagttcgc gcttggcaag tgcgccatgg
     1441 tgcagaacgg caactggggc tacagccaga tcgccggcgt ggagggcaat gtggtgcagg
     1501 agggggatgt ggcgttcctg cccatgtata tgggcctgcc gggggaggag agccagggca
     1561 tcgccatcgg caccgaaaac tttttgtgca tcaacgccca ggcgtccgag gcggaccgga
     1621 aagcgtccct cgattttctc gactggctgt tcagctcgga ggagggcaaa gcgcatgtga
     1681 aaggggagct gggctttatc gccccctttg acacctttac ggcggaggac gcgcccgccg
     1741 acccgctggg ccgcgcggtg gtggagtggc agaaccggga cggggtcaaa aacaccccct
     1801 gggacttcac gatcttcccc agtcagacct ttaaggacaa cttcggctcg gcgctgctgc
     1861 ggtacgccca gggcagcctc gcctgggacg agctggtgag atcagtcgtc gcggactgga
     1921 agacggaaaa agccaatatc gcctgacaag atcctgtcaa agcagcccgc gtgcctgtat
     1981 ttttgcgcgg gaactgtcaa agataaacag cgggtgcggt gagggcttcg gcgcccgcgc
     2041 gcccgctgtt tttcgcgtcc ctcacgggcc gttcgtcccg gccgcagggc ggccctgcgg
     2101 gctgacgcag cgctgcggcg gcagaaactc gtcacttcgg cccgccctga aaaatccgtc
     2161 ttcggaggtt cccctatgca aaaaacgctc aagaaagcgc gctggttcac cgtcttcgtc
     2221 gtccccacgc tgctggcctt tctggcgtcg tttctggcgc cgtttttgat gggcatctat
     2281 ctctctttct gcaagttcac caccgtcaca aacgcccgct gggtggggct tgacaattat
     2341 ctgcgggtct tcaccgaccc cggcggcgac tttgcccacg cgctgggctt tacggcgctg
     2401 ttcacggtgg tgtcggtggt cacggtcaac ctgttcgcgt tcgcgctggc gctgctgctg
     2461 acgcgcgggc tgcggggcac caaccttttc cgcaccgtgt ttttcatgcc gaacctcatc
     2521 ggcggcatcg tgctgggcta tatctggcag atcatcatca acggggtgct gctgggcttt
     2581 ggcgtggata tcacctttga cgcaaaatac ggcttttggg gcctggtggt gctgatgaac
     2641 tggcagctca tcggctatat gatgatcatc tatatcgcgg gcctgcaaaa cgtgccgccc
     2701 gagctcatcg aggccgccga gatcgacggc gcctcgggct ggcagaccct gatccacgtc
     2761 acgctgccca atgtgatgcc ctcggtgacc atctgcacct ttctcaccct gaccaacagc
     2821 ttcaagctgt tcgaccagaa cctggccctc accggcgggg cgccgggcaa gcagaccgcc
     2881 atgctggcgc tggacatcta caacaccttt tacaagcgca ccggctggga gggcgtgggc
     2941 caggccaagg cggtgctctt ctttctcatg gtgggggtca tcgcgtttat ccagctgcgg
     3001 ctcacccgct caaaggaggt ggagaactga tggaaaaggg ctctaaaacc tcccgcgtgc
     3061 tgctcacggt tctgctgctg ctcatcgcgg cgctgttcat cgccccggtg gtcatcgtgg
     3121 tgatgaactc gttcaagggc aacc
//
LOCUS       DBMDYA010000308         3144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6921_length_3144_cov_1.161683, whole genome shotgun sequence.
ACCESSION   DBMDYA010000308 DBMDYA010000000
VERSION     DBMDYA010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6921_length_3144_cov_1.161683"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(625..966)
                     /locus_tag="ACZPFK_08130"
     CDS             complement(625..966)
                     /locus_tag="ACZPFK_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR1406318.1"
                     /translation="MEDCLFCKIAAGEIPSNKLFEDEDILAFYDIDPQAPFHFLVIPK
                     KHYASAAALTEDDAPLLGHIFAVIAKLCAENGCEKGYRVVTNVGEDGGQSVPHLHFHV
                     LAKRSLAWPPG"
     gene            complement(1014..>3144)
                     /gene="alaS"
                     /locus_tag="ACZPFK_08135"
     CDS             complement(1014..>3144)
                     /gene="alaS"
                     /locus_tag="ACZPFK_08135"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1406319.1"
                     /translation="EHGCGKPDCKVGCDCDRYMEVWNLVFSQFDSDGKGTYTLLEHPN
                     IDTGMGLERLACVLQGVDNLFEVDTVRAILNHVERITGKKYGGDHRTDVSIRVITDHI
                     RSTVFMVSDGILPSNEGRGYVLRRLLRRAARHGRMLGVNRPFLAELCDTVIHENGGAY
                     PELREHADYIKKVISAEEERFSRTIDQGLSILNSLMDNVAKAAIEGKRRILSGIEAFK
                     LQDTFGFPLDLTKEIAAEAGIEVDEDTFRAELQKQREKAREDRRKKDISGWSGDLFGE
                     LDAEPTVFVGYDELEANAKVLALSDGEELTEAVSTDDGPKEGVLVVLDRTPFYAESGG
                     QVGDMGYLTADGVKLKVVGCKKTPKGFYVHTCTLESGLLVTGAQLVATVSRRLRMATA
                     RNHTAAHLLQAALREVLGMHVHQAGSYEDAQRVRFDFTHFGAVTAEELRQVEKIVNAK
                     IYDALPVVTRVMPIREAKALGAMALFGEKYGDEVRVVSIDDFSTELCGGTHVTNTAML
                     GGFKILSESSAAAGIRRIEGVTGTNLLALLDERTDELAQIADKLKVGNIADLPVRVAA
                     LNAELHELRSELEKAKAAAANQKVEGLFQSAREVDGLKIFTAYFGGTGTDTLRSMAER
                     LRDEVPGGVTVLCGESEGKCGMAVSVGKEAQARGIKAGALAKLASAVTGGNGGGKPDL
                     AMAGVRDASKVDEALAAVPELVRSLMK"
BASE COUNT          540 a         1092 c          925 g          587 t
ORIGIN      
        1 ggcgatggac atcttatcgc tggccttgca gatgggcagc tcgcgggtga gccctgcgac
       61 ccggagggta taagtatttt ccatagtggc tgatactcct tattcttccg gcggcagtgg
      121 ccgccgtttt aatgacagtt ttttatcggt caacgaaagc taaaagccgg tcgcgagttg
      181 ggcgctgcaa aaacactttt ttctctgcac acaggcaaaa cagtcgcggc cccctcacgc
      241 gcaggcgagg gcggcacaga aatccaccct cccccttttg ccgctccatc cggcccgtcg
      301 ccatcccctt gccagtcgga cgatcccgca aaacgagccc gttatacggg ctcgcatgga
      361 cgcccgctgc gcacgcctaa caatgacgtt aacaggtcgg acaatcccgc aaaacgagcc
      421 cgctgcgcat gcctaacgat gacgccaaca agtcggacga tcccgcaaaa cgagcccgcc
      481 acgcgggctt gcatggacgc ccgctgcacc tgcttaccga tggcatgaag aggtcacacg
      541 atcccgcaaa acgagcccgt tatacgggct cgcatggacg cccgctgcgc atgcctactg
      601 acaacgccag caggcgtcct aatgtcagcc cggaggccag gctaggctgc gctttgccag
      661 cacgtggaaa tgcaggtgcg gcacgctctg accgccgtct tcgcccacat tagtcaccac
      721 gcgatagccc ttttcgcagc cgttctcggc gcagagcttt gcgatcaccg caaagatatg
      781 gcccagcagg ggggcgtcgt cctccgtgag ggcggcggcg gaagcatagt gctttttggg
      841 gatcaccaaa aagtggaacg gggcctgagg gtcgatatcg taaaaggcaa ggatatcctc
      901 gtcctcaaac agcttgttgg aagggatctc ccccgccgcg atcttgcaga acaggcaatc
      961 ttccatcttt tgctctcctt tccatcgctt tgcggcgccg gctcgacgcg cctttatttc
     1021 atcaggctgc gcaccagctc gggcacggcg gccagggctt cgtccacctt ggacgcatcg
     1081 cgcacacccg ccatggccag atcgggcttg ccgccgccgt tgccgccggt gaccgccgac
     1141 gcgagctttg ccagtgcgcc cgccttgatg ccgcgcgcct gcgcctcctt gcccaccgag
     1201 accgccatgc cgcacttgcc ctcgctctcg ccgcagagca ccgtgacgcc cccgggcacc
     1261 tcgtcccgca accgctcggc catgctgcgc agcgtgtcgg tgccggtgcc gccgaaatac
     1321 gcggtgaaga tcttgaggcc gtccacctcg cgcgcactct ggaaaagccc ctccaccttt
     1381 tgattggcgg ccgccgcctt ggctttttcc agctcagagc gcagctcatg cagctcggcg
     1441 ttcagcgccg ccacccgcac cggcaggtcg gcgatgttgc ccaccttgag cttgtcggcg
     1501 atctgcgcca gctcgtcggt gcgctcgtcc aacagggcca gcaggttggt gccggtgaca
     1561 ccctcgatgc gccgaatgcc cgccgcagcg ctgctctcgg agaggatctt gaacccgccc
     1621 agcatggcgg tgttggtcac atgggtgccg ccgcaaagct cggtggaaaa gtcgtcaata
     1681 gagaccaccc gcacctcatc gccgtatttt tcgccgaaca gcgccatggc gcccagggct
     1741 ttcgcctccc ggatcggcat cacccgcgtg accaccggca gcgcgtcgta gattttggcg
     1801 ttgacgatct tttccacctg gcgcagctcc tcggctgtga cggcgccaaa atgggtgaag
     1861 tcaaagcgca cccgctgcgc gtcctcatac gagccggcct gatgcacatg catacccagc
     1921 acctcgcgca gcgccgcctg cagcagatgg gcggcggtgt ggttgcgcgc cgtggccatg
     1981 cgcagcctgc ggctgacggt ggccaccagc tgcgcgccgg tcaccagcag cccgctctcc
     2041 agcgtgcagg tgtgcacata aaagcccttg ggggtctttt tgcagccgac cactttcagc
     2101 ttcacgccgt ccgcggtcag atagcccata tcccccacct gcccgccgga ctcggcataa
     2161 aacggggtgc ggtccaacac caccagcacg ccctctttgg ggccgtcgtc ggtggagacg
     2221 gcctccgtga gctcctcgcc gtcggagagc gccagcactt tcgcgttggc ctccagctcg
     2281 tcatagccca caaagacggt gggctcggcg tccagctcgc cgaacaggtc gccggaccag
     2341 cccgagatat cctttttgcg ccgatcctcc cgcgctttct cccgctgctt ttgcagctcg
     2401 gcgcggaacg tgtcctcgtc cacctcaata cccgcctcgg ccgcgatctc cttggtcaga
     2461 tccagcggga agccgaacgt gtcctgcagc ttgaaagcct cgatgccgga caggatgcgc
     2521 cgcttgccct caatggccgc cttggcgaca ttgtccatca ggctgttcag gatggaaagg
     2581 ccctggtcga tggtgcggga gaaccgctcc tcctcggcgc tgatgacctt tttgatgtag
     2641 tcggcgtgct cgcgcagctc ggggtaagcg ccgccgttct cgtgaatgac cgtgtcgcac
     2701 agctcggcca ggaaaggccg gttcaccccc agcatgcgcc cgtggcgcgc cgcgcggcgc
     2761 agcaggcggc gcagcacata gccccgcccc tcgttggagg gcaggatgcc gtcgctgacc
     2821 atgaacaccg tggagcggat atggtcggtg atgacccgga tcgagacgtc cgtgcggtgg
     2881 tccccgccgt attttttgcc ggtaatgcgc tccacatggt tgaggatggc ccgcaccgtg
     2941 tccacctcga acagattgtc cacgccctgc agcacacagg ccagccgctc cagccccatg
     3001 ccggtgtcga tattggggtg ctccagcagc gtataggtgc ccttgccgtc cgaatcgaac
     3061 tggctgaaca ccaggttcca gacctccatg tagcgatcgc agtcgcagcc caccttgcag
     3121 tccggcttgc cgcagccgtg ctcg
//
LOCUS       DBMDYA010000309         3139 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6929_length_3139_cov_1.566491, whole genome shotgun sequence.
ACCESSION   DBMDYA010000309 DBMDYA010000000
VERSION     DBMDYA010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_3139_cov_1.566491"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="ACZPFK_08140"
     CDS             complement(<1..193)
                     /locus_tag="ACZPFK_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR1406320.1"
                     /translation="MDKHYVIGVDFGTLTARGVLVDVDSGEEAAVSIQGYQDAVIDTA
                     LPGSNRPLPPEFALQNPQDY"
     gene            complement(205..1056)
                     /locus_tag="ACZPFK_08145"
     CDS             complement(205..1056)
                     /locus_tag="ACZPFK_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406321.1"
                     /translation="MNRVLGMFSPEIQRDTVGRLFDAAKVYGFGAIQFDFLSVCGEEM
                     PARIPSSVVEEIRHAADCRGLEICAVNGTFNMIHPSPDAVERGIERFEEIARACAGLG
                     CGLITLCTGTRSTEHMWKPHPGNDAPDAWRDLLHTMERLLALADQYDLTLGVETEASN
                     VVNTPERARLLLDTLKSRRLKIIFDPANLFHPGTACAEKAADIVEHAFDLLKEDIVLA
                     HAKDIRTGEEIAFTSAGRGIVPFRRFLELLDSIGYAGPVVAHGIHDEAEFPFSTAFLN
                     GLMKHQI"
     gene            complement(1053..1988)
                     /locus_tag="ACZPFK_08150"
     CDS             complement(1053..1988)
                     /locus_tag="ACZPFK_08150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1406322.1"
                     /translation="MAYLDVQFYSRVLGLSCTMGVILPEADQGIGVGESVWDGVTPLP
                     VLYLLHGMSDNHTIWMRRTSLDRYAAGRKMAIVMPSAGRSYYSDQKYGLDYLTFIGEE
                     LPAICRRFFRISARREENFIAGLSMGGYGALKVALNYPEAFGYAASMSGVLDLEQLKD
                     IRNCEKAGDEAFLERLKTSDFEAYRSVRDYQLCFGSPAEFRGSPNDLCALVDQLVAEK
                     IPFPQLHLSIGTEDYLYGTNLTFRQKLEENSVKYTYLEFPGVHEWAVWDRTIQTVLDW
                     LPVETNRPTGNGGAERAAQSAANSAVLDGKAGAVQ"
     gene            2452..>3139
                     /locus_tag="ACZPFK_08155"
     CDS             2452..>3139
                     /locus_tag="ACZPFK_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1406323.1"
                     /translation="MAISLNSLDEYQTMMTQCLEEELAKLGGTLVQYNADGSAEKQLA
                     DVESIILTAPDVIVIQAVDPDGTVTAVKTAHASGIPTVVYQLTCNVEAEDFDARVVQQ
                     DPMVRGKVQAAWIKNYLDENPDEVLNVCSLMGAPNGGSNGYKGWQTMYEDEAYQGRVS
                     EIIATDANWSRANAISIVEDWLVTYPEMNCIQAENDEMALGAVTAIKNAGLNPGDYII
                     MGMNGDPEAQK"
BASE COUNT          754 a          847 c          911 g          627 t
ORIGIN      
        1 catagtcctg ggggttctgc agcgcaaatt ccgggggcag aggccggttg gaaccgggca
       61 acgcggtgtc gatgacagcg tcctgatagc cctggatgga gaccgctgcc tcctctccgc
      121 tgtccacgtc gaccaaaacg ccgcgcgctg taagagtgcc gaaatccacg ccgatgacgt
      181 agtgtttatc catagagatc tccctcaaat ctgatgtttc attaacccgt tcaggaaagc
      241 ggtggaaaag gggaactccg cttcgtcgtg gatgccatgg gcgacaacag ggccagcata
      301 gccgatagag tcgagcagct caagaaaacg tctgaaaggg acaatgcccc ggcctgcgga
      361 agtgaaggca atctcctcgc cggtgcggat gtctttggcg tgagccagca cgatatcctc
      421 tttcaacagg tcaaaagcgt gctctacaat gtccgcggct ttttcggcgc aagccgtgcc
      481 ggggtgaaac aggttggccg gatcgaaaat aatttttaag cgcctgcttt ttagagtgtc
      541 cagcagcagc cgggcgcgct ccggcgtatt gacgacattg gaggcttcgg tctccacccc
      601 cagcgtgaga tcatactggt ccgccagagc cagcaaccgt tccatcgtgt gcagcaggtc
      661 ccgccaggca tcgggggcgt cgtttcccgg gtgcggcttc cacatatgct ctgtgctgcg
      721 ggtccccgtg cagagggtta tcaggccgca gccgagtccg gcacaggcgc gggcaatctc
      781 ctcaaagcgc tctataccgc gttccactgc gtcgggggag ggatggatca tgttaaaggt
      841 cccattgacg gcgcagattt ccaggccacg gcagtccgct gcatgacgga tctcctccac
      901 aacagaggag ggaattcggg cgggcatttc ttccccgcaa acggaaagaa aatcaaattg
      961 gattgcgccg aaaccataca cctttgccgc gtcaaagagt cgacctacgg tatctcgctg
     1021 gatctccgga gaaaacattc caagcactct gttcattgca ctgcacccgc ctttccgtcc
     1081 agaactgcgc tgttcgccgc gctttgggca gcccgctcgg ccccgccgtt gcctgtggga
     1141 cggttggtct ctacgggcag ccagtccaac acggtctgta tcgtcctgtc ccagaccgcc
     1201 cattcgtgga cgccggggaa ctcgagataa gtatacttca cgctgttctc ttccagcttt
     1261 tggcggaaag tgagattggt gccgtacaga taatcctcag tgccaatact cagatgaagc
     1321 tgtgggaaag ggattttctc agcgaccagc tgatcgacca gagcacacaa atcatttggg
     1381 gaaccgcgaa attctgcggg agagccgaag cagagctgat aatcccgcac actgcggtat
     1441 gcctcgaagt cagaggtttt cagacgctct aaaaatgcct catccccggc tttttcgcag
     1501 ttgcggatat ccttgagttg ctccagatcc agcacgccgg acatgctggc ggcgtaacca
     1561 aaagcttcgg gatagttgag ggcgactttc agggcaccat atcctcccat ggagagtccg
     1621 gcgataaaat tttcttcccg ccgcgcggag atgcggaaaa agcggcgaca gatggcggga
     1681 agttcctcgc cgataaaggt gagatagtca aggccgtatt tttgatcgga atagtaactg
     1741 cgccccgcgg acggcatgac aatggccatt ttgcggcccg ccgcgtatcg gtccagggat
     1801 gtgcggcgca tccagatggt gtggttgtcc gacataccgt gcagcaggta aagaaccggc
     1861 aaaggggtca cgccgtccca tacggactct cccacgccga tcccctgatc cgcctcaggc
     1921 aaaatgaccc ccatagtaca ggagagcccc agcacacggg aataaaattg aacgtcaaga
     1981 taggccatca agttccctcc taatcattaa aacgatggag ttttgtgttg aaaggcgcgg
     2041 gaaacaccac ttccaattcc agaatatcaa agtgtgaaat aatgtcaact tttaagagca
     2101 aacttgcaaa aatgtgcatc aaaattaaaa aaacgagtaa aatcaataaa aatatgaaaa
     2161 aatgtgcaaa aataagcaaa tatttgacaa tatttatgcg ccgatgctat gttgtggata
     2221 aaggcggcgg gaagctgcga tcaactcact tttgatttgg aggaaaagaa aatgctgaaa
     2281 cacttattca caaaacgcgc attgacagca cttcttctgg cgtgtgccct gacgctttct
     2341 cttgtgggat gcggctcggc ggacagctca aaagtgtcct ctgtcgtgag ctcttcccca
     2401 caggggaccg gggctgacgc agggggctcg gcggcaggca cgaagttcac cgtggcgatt
     2461 tcactcaaca gcctggacga gtatcagacc atgatgaccc agtgtctgga ggaggagctt
     2521 gccaagctgg gagggacgct ggtacagtac aacgcggacg gctcggcgga aaagcagttg
     2581 gcggatgtgg aatccatcat cctcaccgcg ccggacgtca tcgtgatcca ggcggtcgac
     2641 ccggacggca cggtgacggc ggtcaagaca gcccatgcct cgggcatccc cacggtggtc
     2701 tatcagctca cctgcaatgt tgaggcggag gatttcgacg cgcgtgtcgt gcagcaggac
     2761 cccatggtac ggggcaaggt gcaggcggcg tggatcaaaa attatctcga cgaaaatccc
     2821 gacgaggtgc tgaacgtttg ctcgctgatg ggcgcgccaa acggcggcag caatgggtat
     2881 aagggctggc agaccatgta tgaagacgag gcgtatcagg gcagagtgag cgagatcatt
     2941 gccaccgatg cgaactggag ccgggccaac gccatctcga tcgtggagga ctggctggtc
     3001 acctacccgg aaatgaactg catccaggcc gaaaatgatg agatggcctt gggcgcggtg
     3061 actgcgatca aaaatgcggg gctcaatccg ggcgactaca tcatcatggg gatgaacggc
     3121 gacccggaag cccaaaaag
//
LOCUS       DBMDYA010000310         3137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6940_length_3137_cov_1.559289, whole genome shotgun sequence.
ACCESSION   DBMDYA010000310 DBMDYA010000000
VERSION     DBMDYA010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_3137_cov_1.559289"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            56..268
                     /locus_tag="ACZPFK_08160"
     CDS             56..268
                     /locus_tag="ACZPFK_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406324.1"
                     /translation="MKKVIYLCCSALWMLLLMVQHYRGQSFLLEDLVLVISIMTFLAL
                     FAVEYAKSKDREAPSREPRVMMPRKP"
     gene            1057..1878
                     /locus_tag="ACZPFK_08165"
     CDS             1057..1878
                     /locus_tag="ACZPFK_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1406325.1"
                     /translation="MKKFLALLMAAALALSITACGGKDDGADDNVLKVGMECAYAPFN
                     YTQTDDSNNAAEIDGGGWAGGYDVQVAQKIADAMGKKLLIEKMEWDGLIPALTSGKID
                     LIIAGMSPTEERRASIDFSEVYYVSDLVMVVRKDGAYAQAASLEEFSGAKVTGQLNTF
                     HYTVIDQIAGVEKQTAMEDFPTMIVALQSGKIDGYVSERPGAMSAQLSNPDLTYVVFD
                     EGKGFDYSVEEGSIAVGLKKGSELADKVNEALAAISEEERQSMMENAIAHQPAAE"
     gene            1972..2757
                     /locus_tag="ACZPFK_08170"
     CDS             1972..2757
                     /locus_tag="ACZPFK_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1406326.1"
                     /translation="MERGFFDWIAYILQKYGPDILKGAGTTMLIAVVATVAGFAIGLV
                     IGCIRTIPVGPRDGKLKKGLLKVVNFLLNAYVEIFRGTPMMVQAVVIFYGLMEAFRID
                     LSPLSAGLLIVSINTGAYMSEIVRGGIQSLDPGQYEAAHALGLSHWDTMTSVILPQAV
                     RNILPSIGNEFVVNIKDTSVLNVISVTELFFATKTVKGALARTYEPFLICAVVYFVLT
                     FTTTRLLRLIEKKMDGPDSYTICGSGSAADAAIKLPRKKEVTD"
     gene            2757..>3137
                     /locus_tag="ACZPFK_08175"
     CDS             2757..>3137
                     /locus_tag="ACZPFK_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1406327.1"
                     /translation="MEDVICIQGLRKSFGANEILKGVDFSVSKGEVVCLIGASGSGKS
                     TMLRCINLLETPSGGEIRFHGQNILELGKERRAYCAKLGMVFQSFNLFDNLTVLQNCV
                     MPQLKVLHRTKPQAEQTAREYLAKV"
BASE COUNT          707 a          873 c          964 g          593 t
ORIGIN      
        1 ccacatataa gaataaggaa agaaccagtc tgcggtggcg ggaggaggac atccggtgaa
       61 aaaagtgatt tatctctgct gttcggcgct gtggatgctg ctgctcatgg tgcagcatta
      121 ccgggggcaa agctttctgc tggaggatct ggtcctcgtg atcagtatca tgacgttttt
      181 ggccctcttc gccgtggagt acgccaaaag caaggatcgt gaggcgccgt cccgcgagcc
      241 ccgcgtcatg atgccaagaa agccctaaaa gaaaagggca gccgcaaagg gcgcgttttg
      301 gcaagagaga accgcccccc gagagaagaa ttttcgcgca gagcgccgtc acatccaccc
      361 ggcagcagca aaagggaggc ggttttgccc tgcgagctgc cggcgcgagc ggacggtcag
      421 cgtgaatttt tcttgctgtg catcgcagct tccctccctc aggggcgcac ggcggttttg
      481 attcggggat tttcaaagta aacacagtgc aaaaaagggt tggcaatctc tgcggattga
      541 caatcctttt tgaatggata gagttatcaa agctgtcggt ccaaaactga tattttctta
      601 ccgcaacacg cctaaagggg cgttcctttt attttaaggt ctgcgcgagt tcgcgcgccg
      661 tgcctgccgg ggcggaaact gccgccgctg aggccccagg gagaaaagcc gtctccggca
      721 caaggccggg cggcacccgg cggcggccgc ggcgggcctg agaggcgaac agagaaaaag
      781 gaacggcagg ggaagccggc tcccaggaaa atcaagataa aaaacatcca aaaatttgaa
      841 aaaagagtcg acaaaatgtc taaaacaagc cgttgtcaag cgcttccaga ggaaaaatca
      901 gacaaaaaat atcgccgcaa tttaaaaatg ttgcataatc ccggcacatg ccttaaaatg
      961 aaagagtact tctgctgact aaagcgtttt agaggcggag ggggaggcgt ggacgcgccg
     1021 atctacggat cagagaaaaa agagggagtg gttcgcatga aaaaattcct ggctttgctg
     1081 atggccgccg cgctggcgct ttccataact gcatgcggcg gcaaggacga cggggcggac
     1141 gacaatgtgc tcaaggtggg catggagtgc gcctatgcgc cgttcaatta tacccagacc
     1201 gacgactcca acaacgcggc cgagatcgac ggcggcggct gggcgggcgg ttacgacgtg
     1261 caggtggcgc agaagattgc cgacgccatg ggcaaaaagc tgctcatcga aaagatggag
     1321 tgggacgggc tcatcccggc gctgacctcg ggcaagatcg acctgatcat cgcgggcatg
     1381 tcgcccacgg aggagcgcag ggcttccatc gatttttcgg aggtctatta tgtctccgac
     1441 ctagtgatgg tggtgcgcaa ggatggcgcc tatgcgcagg ctgcgtcgct ggaggagttc
     1501 agcggcgcca aggtcaccgg gcagctgaac acgttccatt acacggtgat cgaccagatc
     1561 gcgggtgtgg aaaagcagac cgccatggag gatttcccca ccatgattgt ggcgctccag
     1621 tcgggcaaga tcgacggcta tgtctccgag cgcccgggcg ccatgtcggc ccaactgtcc
     1681 aacccggacc tgacctatgt ggtctttgac gagggcaagg gctttgacta cagcgtggag
     1741 gagggctcca tcgccgtggg cctcaaaaaa ggaagcgagc tcgccgacaa ggtcaacgag
     1801 gcgctcgccg ccatcagcga ggaggagcgc cagtccatga tggaaaatgc catcgcccac
     1861 cagcccgccg cagaataggc gcgcagcggc ggcaccaagc ttttcaaaag gcgctatccg
     1921 ggccccggcc cggataggct ttttgttatc tgaaaaaagg agctgaagcc catggaacgc
     1981 ggctttttcg attggatcgc ctacatcctg caaaagtatg gccccgatat tttaaaaggg
     2041 gcgggcacca ccatgctgat cgccgtcgtg gcgaccgtcg ccggctttgc cattgggctg
     2101 gtgatcggct gcatccgcac catccccgtc ggcccccggg acggaaagct gaaaaagggc
     2161 ctgttgaaag tggtcaattt tctgctcaac gcctatgtgg agatcttccg gggcaccccc
     2221 atgatggtgc aggccgtggt catcttttac ggcctgatgg aggcgttccg catcgatctc
     2281 tcgcccctct cagcaggcct gctgatcgtt tcgatcaaca ccggcgccta catgtccgag
     2341 atcgtgcgcg gcggcatcca gtcgctggac ccggggcagt acgaggcggc ccatgcgttg
     2401 ggcctctccc actgggacac gatgaccagc gtcattttgc cgcaggcggt gcgcaatatc
     2461 ctgccctcca tcggcaacga gtttgtggtc aacatcaagg acacctcggt gctcaacgtc
     2521 atcagcgtca ccgagctgtt ctttgccacc aaaacggtga aaggcgcctt ggcccgcacc
     2581 tacgagccgt tcctcatctg cgcggtcgtt tattttgtgc tcactttcac caccacccgc
     2641 ctgctgcggc tgatcgagaa aaagatggac ggcccggaca gctataccat ctgtgggtcc
     2701 ggctcggcgg cggacgcggc gatcaagctg ccgcgtaaaa aggaggtcac cgactgatgg
     2761 aggacgtcat ctgcatccag ggcctgcgca aatcgttcgg cgccaacgag atcctgaaag
     2821 gcgttgattt ttcagtgagc aagggggagg tcgtctgcct gatcggcgcc tccggcagcg
     2881 gcaagtccac catgctgcgc tgcatcaacc tgctggagac gccgtcgggg ggtgagatcc
     2941 gctttcacgg tcaaaacatc cttgagctgg gcaaagagcg ccgcgcctac tgcgccaagc
     3001 tggggatggt gttccagagc ttcaacctgt tcgacaatct gacggtgctg cagaactgcg
     3061 tgatgcccca gctcaaggtg ctgcacagga caaagccgca ggcggagcag accgcccggg
     3121 aatatctggc aaaggtg
//
LOCUS       DBMDYA010000311         3131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6955_length_3131_cov_1.840264, whole genome shotgun sequence.
ACCESSION   DBMDYA010000311 DBMDYA010000000
VERSION     DBMDYA010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_3131_cov_1.840264"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..1051
                     /locus_tag="ACZPFK_08180"
     CDS             209..1051
                     /locus_tag="ACZPFK_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1406328.1"
                     /translation="MGCIYSVTALGVNIIYGIMQIVNWAHGSILMISMFIVYGAVVLL
                     GMNPYLALILAVPIMFVFGYVFQHVVIRPLFLREKADSNPVGLLLCTAGLGIALENIM
                     LMIFKSTPRSISMGISLTYVPIGFGRKLSLVKIIGAGIAVAVVAIVWIYMNKTYSGKA
                     LRATSQDREAAQMMGINVLKCYNIAFGIGCAICAVAGVIFSTFYTITPSVGTTIGSKA
                     FIILVLGGKGSVGGCLIAGLIIGLVEAFGGLFFDSYTGQMLCFVIFVLMLYFKPNGLF
                     GKKV"
     gene            1064..2071
                     /locus_tag="ACZPFK_08185"
     CDS             1064..2071
                     /locus_tag="ACZPFK_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1406329.1"
                     /translation="MKQLSKPALIKAAVSLALIAVFLVAVQFLNSYYQQIFIYILMFA
                     YFASAWNILSGFAGQFSLGHAIFIGIGAYTSTILYTQFGVSPWIGMLAGGVLAAGVGV
                     LIGLPCFKLKSTYFTLTTIALANILLLLVQNTRSIGMLAINGARGIQIPAVTGNDFGA
                     MQFLDKKYYLYIILAMLGVVLIVCQWIKSTKMGYQLAAVANNQDAAESLCVNSRALKL
                     KAMAISAFLCAMGGTFYAQVILFCNPARLFSEALSDKLAIVAMLGGKGTVFGPTAGAL
                     IVETISQFASAKFANIQGLNLAIYGIMLIVCIRFFPDGILPIIQKFVRRVFKKKGEVE
                     K"
     gene            2068..2793
                     /locus_tag="ACZPFK_08190"
     CDS             2068..2793
                     /locus_tag="ACZPFK_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406330.1"
                     /translation="MNDILQVEHLSKHFGGLKAVNDFSMTVKEGEIHALIGPNGAGKT
                     TLFNLISGFIPPSEGKVIFDGQDVTNAKPYHLCSKGLVRTYQIVQPFRGMSTLDNVMV
                     GGFTHTDKTAAAREKAQKALEITKLDHKAGVQARSLNLCEQKRLEVARALATEPKLIM
                     LDEVMAGLNPTEVAEVMEIVMNIKALGITIVVIEHIMQAVMNISDTITVLSFGEKIAE
                     GRPDEIVADPDVISVYLGSDYAQ"
     gene            2848..>3131
                     /locus_tag="ACZPFK_08195"
     CDS             2848..>3131
                     /locus_tag="ACZPFK_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1406331.1"
                     /translation="MKKLLAVLLICAMASSMAACGSDASSGGGTASPGSGGGTGSGGD
                     VIKLGVLFPYTGSAAAVAEDAQKGMEFAISEINDRGGIASMGGKKIELYYG"
BASE COUNT          679 a          803 c          916 g          733 t
ORIGIN      
        1 gcggcgggcc ttacctgggg gatggcggat ggggaggcag cggattgggc gaaccgctac
       61 agcgcggtga tggtcagcag aaaaggcgcc atcctgtctt accccacctt gaacgaagtg
      121 aaagagattt ctggcagttt caaaaaacgc caggcaaatg aaggagcctg aaaagatgta
      181 ttatgaaaca ctgatactcg gactggtcat ggggtgcatc tatagcgtaa ccgcgctggg
      241 ggtcaacatc atttacggga tcatgcagat cgtcaactgg gcacacggat ccatcctgat
      301 gatctcgatg tttatcgtat acggagccgt cgtcctgctg ggcatgaacc cgtatctggc
      361 cctcatcctt gcagtcccca tcatgtttgt ttttggatat gtatttcagc atgtcgtcat
      421 ccggcctctg tttttgcgcg aaaaagcgga ttcaaacccg gtcggcctgc tgctttgcac
      481 cgcgggcctg ggcatcgcgc tggaaaacat catgctgatg atcttcaaat ccacaccgcg
      541 ctcgatctcc atgggtattt cgctcactta cgttcccatc gggttcggca gaaagctcag
      601 tctggtcaag atcatcggcg ccggcattgc ggtggcggtg gtcgccatcg tctggatcta
      661 tatgaataag acctattccg gaaaagccct ccgcgcgacc tctcaggacc gggaggcagc
      721 ccagatgatg gggatcaatg ttctcaagtg ctataacatt gcatttggca tcggctgtgc
      781 gatctgcgcg gtggcagggg tcattttttc caccttttac acgattacgc catcggtggg
      841 cacaacgatc ggcagcaagg cttttatcat tttggttctc ggcggcaagg ggtctgtggg
      901 cggctgcctg atcgcagggc tgatcatcgg cctggtggag gcctttggcg ggctgttctt
      961 cgattcttat acgggccaga tgctgtgctt tgttatcttc gtcttgatgc tttacttcaa
     1021 gcccaacggt ttgtttggaa agaaggtgta ggagatgaca aaaatgaaac agctttcaaa
     1081 gcccgcgctt atcaaggcgg cggtgtcgtt ggctctgatc gcggtatttc tggtggcggt
     1141 ccagtttttg aacagttatt atcagcagat cttcatctac atattgatgt ttgcctattt
     1201 tgcttcggcc tggaacatcc tgagcggctt tgcgggccaa ttctcgctgg ggcatgccat
     1261 ctttatcggg atcggggcct acacctctac catcctttac acccagttcg gcgtttcccc
     1321 ctggatcggg atgctggccg gcggagtgtt agctgcaggt gtgggggtgc ttatcggttt
     1381 gccctgcttc aaactcaaga gcacctattt caccttgacg acaatcgcgc tggcaaacat
     1441 tctgcttctg ctggtgcaga atacccgcag tatcgggatg ctggcgatca acggcgcccg
     1501 gggcatccag atccccgcgg tcactggcaa cgattttgga gcgatgcagt ttttggataa
     1561 aaagtattat ctctatatca tccttgccat gctgggggtc gtactgattg tttgccagtg
     1621 gatcaagagc acaaagatgg gatatcaact ggcggcggtt gcaaacaacc aggatgccgc
     1681 ggagtccctc tgcgtcaact cgcgggcgct gaagctcaaa gcgatggcga tcagcgcttt
     1741 cctctgcgcc atgggaggca ctttctacgc tcaggttatt ctgttctgca acccggcgcg
     1801 gctcttttcg gaggcgttgt cggacaagtt ggctatcgtt gccatgctgg ggggcaaagg
     1861 aactgttttc ggccccacgg cgggtgcgct gatcgtggag acgatctccc aatttgcctc
     1921 tgcaaagttt gccaatatcc agggtctcaa cctggcgatc tacggcatca tgttgatcgt
     1981 ctgcatccgc ttcttccccg acggcatcct gcccatcatc caaaagtttg tgaggagggt
     2041 cttcaaaaag aagggggagg tagaaaaatg aacgatatcc tgcaagtgga gcatctgtcc
     2101 aagcactttg gaggcctgaa agcggtaaat gatttttcca tgacggtaaa agagggggag
     2161 atccatgcgc tgatcgggcc gaacggggcc ggtaagacga cgctgttcaa cctgatctcc
     2221 ggctttatcc ccccgtcgga gggaaaagtg atatttgatg ggcaggacgt caccaacgcc
     2281 aagccctatc atctctgcag caagggcctg gtgcgcacct accagatcgt gcagcctttc
     2341 cgcggcatgt ccacactgga caatgtgatg gtgggcggat ttacccatac ggacaagacc
     2401 gcggcggccc gagaaaaagc acaaaaagct ttggagatca ccaagctgga ccacaaggcc
     2461 ggcgtccagg ccagaagcct gaacctctgt gagcaaaagc gcttggaggt cgcgcgcgcc
     2521 ctcgccaccg agcccaagct gatcatgctg gacgaggtga tggctgggct caaccccacg
     2581 gaggtcgctg aggtgatgga aatcgtgatg aacatcaaag ccctggggat caccatcgtg
     2641 gtcatcgagc acatcatgca ggcggtgatg aatatttccg atacgatcac ggtcctctct
     2701 tttggggaaa agatcgcaga ggggagaccg gatgaaatcg tggcggaccc ggatgtcata
     2761 tcggtatatc tggggtccga ttatgcgcag tgatggacct tgcctgcaaa gcaggtgaaa
     2821 taaacaaaac caaaaggaga aatgacgatg aaaaagctac tggcagttct tttgatctgt
     2881 gcaatggcat cttccatggc cgcatgcggt tccgatgctt ccagcggtgg cggcacggcc
     2941 tccccggggt ctggaggagg gaccggatcg ggcggcgacg tgatcaagct gggggtgctg
     3001 ttcccctaca cgggctccgc cgcagccgtg gccgaggacg cgcaaaaagg gatggagttc
     3061 gccatttctg agatcaatga ccgcggaggc atcgcctcta tgggcggaaa aaagatcgag
     3121 ctctattatg g
//
LOCUS       DBMDYA010000312         3131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6956_length_3131_cov_1.473927, whole genome shotgun sequence.
ACCESSION   DBMDYA010000312 DBMDYA010000000
VERSION     DBMDYA010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6956_length_3131_cov_1.473927"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            264..2186
                     /locus_tag="ACZPFK_08200"
     CDS             264..2186
                     /locus_tag="ACZPFK_08200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1406332.1"
                     /translation="MNNWIVEPELVACAVIALLLCFSREKRLAPTLQSRIYRACLFFA
                     LFSIALNIITIALLRTPGRLPYALNYLLNLLYFIFVGLMELALTLYVMYLIQGRNRCF
                     ARAVWVCGALQAAFLALAATNHLTGLLFTLEDGGAYRRGPGNRAIYLLLVAYSALVAF
                     CYLRQRREVSRQVRRVIRVLPLVALMMGTVQYCFPDVMMSGSVMACILLVLFLNFQSR
                     RQRTDSLTGLGSREGFFDTAEQLFARGRPFYCIGITLRHFSEVNNKLGHTGGDQVLCA
                     VAEYLEAFGRRGNVCRLDGAEFALLLPDQGEEAGAALLRRLADRFAQPWPQGKNIRLE
                     ARLVGVRCPEHGKDANELALHLEYGDMQLKKQPGTCLMCFGDDEQAALQRRNEVKALI
                     ERSMARGGFEVVFQPIYDCAKKRFCTAEALLRLRDERGEPVRPDEFIPLAEEFGLIVE
                     LDWMVLELVCGFLQQNPDLPIEAVSVNFSVQQFEEQDAAGRVLRTLARHGIAPSRLKL
                     ELTERVLARDLPRTSRVLQQLCDGGVGLYLDDFGTGYSNLASVARLPLEAVKIDKSLL
                     VEAQKSPETALLLDAVVRTFHTMGRRVVLEGLESAGQLRRIAGLPIDAVQGYFYARPM
                     PGDQYRTLLLGQAAQV"
     gene            2354..2725
                     /locus_tag="ACZPFK_08205"
     CDS             2354..2725
                     /locus_tag="ACZPFK_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1406333.1"
                     /translation="MTAKPLTEAELRLAELIWDAQPLPSGELVRRCEEAFGWKKSTTY
                     TMLKKLCGCGVTRNEGAIVTARISREEYHRRQGEAVIEEGFDGSLPRFLTAFMQGRGL
                     SRREAEELKELIDRYEEARDE"
     gene            2718..>3131
                     /locus_tag="ACZPFK_08210"
     CDS             2718..>3131
                     /locus_tag="ACZPFK_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1406334.1"
                     /translation="MSEMFKTVLQMSLQGSYVILLVLAARLALRRAPKGLSYALWSVA
                     LFRLLCPVTLQSVFSLLPRGQVLAAPVERLAAAPGFITPTTVAPQTAGRGYIGLSANY
                     IDPMAICALIWLVGAAALLLGSLISVLRLRRSLRGA"
BASE COUNT          538 a          911 c         1050 g          632 t
ORIGIN      
        1 accggtaatt tgtccggccc ggcacaagaa cgttttctca ccccctttcc ttttcgcgcg
       61 gtaaaaaagg agggctcccg ccctgttggc tattgccccc caaaagggta aaaaaacctt
      121 tacagcttac ggatttatat gaaaaaactg gattttgctt ttaaaccagt gtatgataag
      181 aatataagtt atacatattg ctgaaatttg tagaaaaata taggttttga gactttaccc
      241 ttgctttttc ttcggagtat gccatgaaca actggattgt tgaacccgag ctggtcgcct
      301 gcgcggtcat cgcgctgctg ctgtgctttt cacgcgaaaa gcgcctggcg cccaccctgc
      361 agtcgcgcat ttaccgcgcc tgccttttct tcgcgctgtt ctccatcgcg ctcaatatca
      421 tcaccatcgc cctgctgcgc acgccggggc ggctccctta tgccctgaat tatctgctca
      481 atctgctcta tttcatcttt gtgggcctga tggagctggc cctcaccctg tatgtgatgt
      541 acctgatcca ggggcgcaac cgctgttttg cgcgggccgt ctgggtgtgc ggggcgctgc
      601 aggccgcttt tttggcgctt gcggccacca atcatctcac cggcctgctg ttcaccctcg
      661 aggacggcgg cgcctatcgc cgcggccccg gcaaccgggc aatctatctg ctgctggtgg
      721 cctacagcgc cctggtggcc ttttgctatc tgcgccagcg gcgcgaggtg agccggcagg
      781 tgcgccgcgt tatccgggtg ctgccgctgg tggcgctgat gatggggacg gtgcagtatt
      841 gcttccccga tgtgatgatg agcggctcgg tcatggcctg catcctgctg gtgctgtttt
      901 tgaattttca gagtcggcgc cagcgcaccg actcgctcac cggcctgggc agccgggagg
      961 gctttttcga cacggccgaa cagctctttg cgcgcggacg gcccttttac tgcatcggga
     1021 tcaccctgcg ccatttcagc gaggtgaaca acaagctggg ccacacgggc ggcgaccagg
     1081 tgctgtgcgc ggtggcggaa tacctggagg cctttggccg ccggggaaat gtctgccgac
     1141 tggatggggc ggaatttgcg ctgctgctgc cggaccaggg ggaagaggcc ggggcggcgc
     1201 tgctgcggcg gttggcggac cgctttgcgc agccctggcc gcaggggaaa aatatccgtc
     1261 tggaggccag gctggtgggg gtgcgttgcc ccgagcacgg caaagacgca aacgaactgg
     1321 cactgcatct ggagtatggc gacatgcagc tgaaaaagca gccgggcacc tgcctgatgt
     1381 gctttgggga cgacgagcag gccgcactgc agcggcgcaa cgaggtaaaa gccctgatcg
     1441 agcgctccat ggcccggggc gggtttgagg tcgtgtttca gcctatctac gactgtgcaa
     1501 aaaagcggtt ttgcaccgcc gaagcgctgc tgcgcctgcg ggacgagcgg ggcgagccgg
     1561 tgcggccgga tgagttcatc ccgctggcgg aggagtttgg gctgatcgtg gagctggact
     1621 ggatggtgct ggagctggtg tgcggttttt tacagcagaa cccggacctg cccattgagg
     1681 cggtctcggt gaacttttcc gttcagcagt ttgaggagca ggatgccgcc gggcgggtgc
     1741 tgcggaccct tgcccgccac gggatcgcgc cgagccgcct caagctggag ctgaccgagc
     1801 gggtgctcgc ccgcgatttg ccccgcacaa gccgggtgct gcaacagctc tgcgacggtg
     1861 gggtggggct ttatctggac gatttcggca ccggctattc gaacctggcc tcggtggccc
     1921 ggctgccgct ggaggccgtc aaaatcgaca agagcctgct ggtggaggcg cagaaaagcc
     1981 cggagaccgc cctgctgctg gatgcggtgg tgcgcacgtt ccacaccatg ggccgccgcg
     2041 tggtgctgga ggggctggag agcgccgggc agctgcggcg catcgccggc ctgcccatcg
     2101 acgccgtgca gggatatttt tacgcccgcc ccatgcccgg cgaccaatac cgcaccctgc
     2161 tgctgggaca ggccgcgcag gtgtgagcgt gccggaaaaa ggcaaaacag agaaaaacga
     2221 gaaagcgttt tgtgcatctg cccaaaacgc ttcttttttg ttgacaaggt ggtctataag
     2281 ttatagacta aaaatacggc aaaggtctat aagctgtaaa ccaaaatgga acggggaaag
     2341 gggagtggag cggatgacgg caaaaccgct caccgaggcg gagctgcggc tcgcagagct
     2401 gatctgggac gcccagccgt tgccctccgg ggaactggtg cggcgctgtg aggaggcttt
     2461 cgggtggaaa aaatccacca cctacaccat gctgaaaaag ctctgcggct gcggcgtcac
     2521 ccgcaacgag ggcgccatcg tcaccgcgcg catcagccgg gaggagtatc accggcgcca
     2581 gggagaagcg gtgatcgaag agggcttcga cggctcgctg ccgcgctttt tgacggcgtt
     2641 catgcagggc agggggctgt cccgccgcga ggcggaggag ctgaaagagc tgatcgaccg
     2701 ctatgaggag gcgcgagatg agtgagatgt tcaagaccgt gttgcagatg agcctgcagg
     2761 gcagctatgt gattttgctg gtgctggccg cccggctggc gctgcgccgc gcgcccaagg
     2821 ggctgtccta cgcgctttgg tctgtggcgc tgttccgcct gctctgcccg gtcacgctgc
     2881 agtcggtctt cagcctgctg ccccgcggcc aggtgctggc cgcgccggtg gagcggctgg
     2941 ccgcggcgcc gggctttatc accccgacga cggtggcccc ccagacggcg gggcggggat
     3001 atatcggcct ttccgccaat tatatcgacc cgatggcgat ctgcgccctg atttggctgg
     3061 tgggcgcggc ggcgctgctg ctgggcagcc tgatctcggt gctgcgcctg cgccgctcgc
     3121 tgcgcggggc c
//
LOCUS       DBMDYA010000313         3129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6959_length_3129_cov_1.506935, whole genome shotgun sequence.
ACCESSION   DBMDYA010000313 DBMDYA010000000
VERSION     DBMDYA010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_3129_cov_1.506935"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="ACZPFK_08215"
     CDS             complement(<1..726)
                     /locus_tag="ACZPFK_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1406335.1"
                     /translation="MKAELISVGTELLLGDILNTNAQYLSKELAALGITVHRQSVIGD
                     NPDRLAQLVEDAKGRSELLLFTGGLGPTGDDLTKETVAGVFHDELVLDKGELDKIRMY
                     FECRGGMPHNNEKQAMVPRRGQKIENTCGTAPGVLFRDEGCTAILMPGVPHEMRAMFE
                     RSVRGLLMELAGGAIVSTMLHIFGPGESSLEEMVGDLLQGANPTAALYAKNCEVAVRV
                     TARGADEKEAAALRDGLVQRFRDR"
     gene            complement(881..2053)
                     /gene="thiI"
                     /locus_tag="ACZPFK_08220"
     CDS             complement(881..2053)
                     /gene="thiI"
                     /locus_tag="ACZPFK_08220"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1406336.1"
                     /translation="MRELIMAYQGEMALKGLNRKTFEVAAMKTIRKRLADLGRFKVYD
                     AQSTIYVEPEDEDADMDGAYARMQKVFGIAALSRAAVCGKNFEEICAVAKEYLAEALR
                     GARTFKVVARRSDKTFPMTSMELACELGGVLLEAFPHLEVDVHHPDCQVFVEIRDYAA
                     YVHPDKVKGAGGMPTSTSGKASVLLSGGIDSPAAAWMMAKRGLTIQGIHFASPPYTSE
                     RARQKVVDLAYKLMPWTGPFKLFVVPFTEPQVYIRDHGVPVLFTVLMRRSMMRVAEKL
                     SVRYGADALITGESLAQVASQTLKAIVCTDAAQELPVLRPLIGMDKSEITDIARRIDT
                     YDISIQPFEDCCTIFTPPHPKTRPSLEEILAAEEAMPELFALEEAAAAGAEKLHIE"
     gene            complement(2921..3121)
                     /locus_tag="ACZPFK_08225"
                     /pseudo
     CDS             complement(2921..3121)
                     /locus_tag="ACZPFK_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
BASE COUNT          553 a         1051 c          871 g          654 t
ORIGIN      
        1 ccggtcgcga aagcgctgca ccagcccgtc gcgcagcgca gcggcctctt tttcatccgc
       61 cccgcgcgcg gtgacccgca ccgccacctc gcagtttttg gcatagagcg ccgccgtggg
      121 gttcgcccct tgcagcaggt cgcccaccat ctcctccagg ctggactcgc ccggcccaaa
      181 gatgtgcagc atggtggaga cgatcgcccc acccgccagc tccatcagca agccccgcac
      241 cgagcgctcg aacatggccc gcatctcgtg gggcaccccc ggcatcagga tggcggtgca
      301 gccctcgtcc cggaacagga cgcccggcgc cgtgccgcag gtgttttcga tcttttgccc
      361 ccgcctgggc accatggcct gcttttcgtt gttgtggggc atgccgcccc ggcactcgaa
      421 gtacatcctg atcttatcca gctcgccctt gtccagcacc agctcatcgt gaaagacccc
      481 ggccactgtc tccttggtca ggtcgtcccc ggtggggccg aggccgccgg tgaacagcag
      541 cagctcgctg cgccccttgg cgtcctccac cagctgggcc aaacggtcgg ggttgtcccc
      601 gatcacgctc tgccggtgca cggtgatgcc cagcgccgcc agctctttgg agagatactg
      661 ggcgttggtg ttgaggatgt caccgagcaa cagctcggtg cccactgaga tcaattccgc
      721 tttcatggac gctccttcgc cgctctctca acggccggtc cggccccggg ggagagcttt
      781 cttttttaca aattttttct gggaaacggg gcgcagctcc gtttgccgca gacgccgcgc
      841 tctccatcag gtaattattt ccattaaaac taccagttgt ttattcgatg tgcagctttt
      901 ccgcgcccgc cgcggcggct tcttccagcg caaacagctc gggcatcgcc tcctccgccg
      961 ccaagatctc ctccaggctg gggcgcgtct tggggtgggg cggcgtgaag atggtgcagc
     1021 agtcctcaaa gggctggatg ctgatgtcat aggtgtcgat gcgccgggca atgtcggtga
     1081 tctcgctctt gtccatgccg atcagcggac gcagcaccgg cagctcctgg gccgcatcgg
     1141 tgcagacgat ggctttcaag gtctggctgg ccacctgggc caggctttcg ccggtgatca
     1201 gcgcgtcggc gccataccgg acgctcagct tttccgccac ccgcatcata ctgcggcgca
     1261 tcagcaccgt gaacagcacc ggcaccccgt ggtcgcggat atagacctgg ggctcggtga
     1321 acggcaccac aaacagcttg aacggcccgg tccagggcat cagcttatag gccaggtcca
     1381 ccactttttg tctggcccgc tcgctggtgt agggggggct ggcaaagtgg atgccctgga
     1441 tggtcaggcc ccgctttgcc atcatccagg ccgccgccgg agagtcgatg ccgccggaga
     1501 gcagcacgct cgccttgccg gaggtggagg tgggcatgcc gcccgcgcct ttcaccttgt
     1561 ccggatgcac ataggccgca tagtcccgga tttccacaaa gacctggcag tccgggtgat
     1621 ggacgtccac ctcaagatgc ggaaaggcct ccagcaatac gccgcccagc tcgcaggcca
     1681 gctccatcga ggtcatgggg aacgtcttgt cgctgcgccg cgccaccacc ttgaaagtcc
     1741 tggccccgcg cagagcttcc gccagatact ctttcgccac ggcgcagatt tcctcgaagt
     1801 ttttgccgca gaccgccgcc cgggagaggg cggcgatgcc gaaaaccttc tgcatgcgcg
     1861 cgtaggcccc gtccatatcc gcgtcctcgt cctccggctc cacatagatg gtgctctgcg
     1921 cgtcgtagac cttgaagcgg cccaggtccg ccagacgttt gcggatggtc ttcatcgccg
     1981 caacctcaaa agttttgcgg ttgaggcctt tcagcgccat ctccccctgg taggccatga
     2041 tcaattctct catagtcgtc ttccctttgc ttcgtttgta ttttttatcc tctttttccg
     2101 gagatcaaaa cgcaggcgcg gggcccgcgg agaacctaca gataaacgcg gtgggcggta
     2161 aagacccggt gcgaaaaaaa tgattttcag gcacacaaaa ccccgccgcc tctttttttc
     2221 catgtcaacg tcgctgggcc acttggtcgc ccggcaccag ctcttttctc cctctgcgtt
     2281 ttaccgcagc ccctggatgc caaatctcac aggatgaaaa acagcctcat tcctataaag
     2341 ctgataaaat gggcgatctg cgcataggcc acagtttccc ctcaaatgcc ccattaacaa
     2401 cgttgaatct cccatcaagt tcctcagaaa agtccgtcca cgccctcatg ggtcttagaa
     2461 tggggttcaa atgaaaaagg accatttcag gtggatcgtt tccaacgaag tggggggctg
     2521 taattctcac tcgctcctgc aacagaaaag gccgaaatac ccttactgaa cgattctcct
     2581 tgtccccata tactccattt aaaaccggcc gacaagccca tgttcgagtt ttgcgacgcc
     2641 gcttttgggg aaagagtgat cgaatttcgc ccctcaagct gtgcgtttgc acttcagaaa
     2701 gccgcttctt ccgcagccct cagaagaatg cgtctgcgcc atgcgcggac acgccgacaa
     2761 gcacgctcct ccgcgggctt ttgtcgatct tttggcctcc ggccagtcct ggccaaagag
     2821 caggccgcca cgctcttctc ttttccgctt gcgtccaaaa cggcggtgtt tccactgggc
     2881 cagaccgcct ctagcgtcgc tgcagcatcg tcagcccctc gtccaggccg tccagcagag
     2941 cggtcaccgc cgcctcgtcc tccgggtcca ctccggcccg gtaggcccgc agacctacgg
     3001 tccgatagat ggccccggta tccacataca aaaagcccag cctgcgggcc agcagcttcg
     3061 ccagggtgga ctttcccgcc ccggaggggc cgtccacggc gatgctcttg tgctttgtca
     3121 tgggttgat
//
LOCUS       DBMDYA010000314         3125 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6971_length_3125_cov_1.562169, whole genome shotgun sequence.
ACCESSION   DBMDYA010000314 DBMDYA010000000
VERSION     DBMDYA010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6971_length_3125_cov_1.562169"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="ACZPFK_08230"
     CDS             complement(<1..512)
                     /locus_tag="ACZPFK_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1406337.1"
                     /translation="MSTKFFTNCNIVDVVNEKVFAGSVVVEGSQITEVGAELTCPQGA
                     EVVDLGGKWLMPGLFNCHTHMCLAPDADPKYDKNDCQITMMALENLKKFVDSGCTFIR
                     DVGGMNYIDIDIRNEAKKGKLVAPDMQVAGKCICMTGGHGWMMGREADGVDDCRKAAR
                     EQLRAGADWI"
     gene            complement(737..1156)
                     /locus_tag="ACZPFK_08235"
     CDS             complement(737..1156)
                     /locus_tag="ACZPFK_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HIR1406338.1"
                     /translation="MRRPARFSRSRRAAAAPILSAEAAAACGAKCGVWSKKRRARNKK
                     FSGTTRRSVPMFESRCGICCNDCERKAEVHCTGCLSMALPFWGGECPVKSCCEGRGLD
                     HCGLCPEFPCGVLSSMGADQGYDPAPRLRQCRLWTKE"
     gene            complement(1092..1712)
                     /locus_tag="ACZPFK_08240"
     CDS             complement(1092..1712)
                     /locus_tag="ACZPFK_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1406339.1"
                     /translation="MNVLLLNGSPHRHGCTAVALDEVAKTLAECGVESETMWIGAKPV
                     RGCIACGGCRGKGRCVFDDDPANLLIEKMAQADGLVVGSPVYYASANGSLVSLLDRAF
                     YACGGFAGKPAAAVASARRAGTTVTLDELNKYFTICGMPVVSSTYWNMLHGNTAEEAC
                     RDAEGLQTMRNLARNMAWLLRCIEAGAQAGAVFPQQEGGSRTNFIR"
     gene            complement(2148..>3125)
                     /locus_tag="ACZPFK_08245"
     CDS             complement(2148..>3125)
                     /locus_tag="ACZPFK_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1406340.1"
                     /translation="YYIKARFKGRFGSFLAGFFSVAIIVALGFMGNMVQSNSISDAFQ
                     KAFHTPPLLMGVLVAAAAAFIFLGGKRRIASVTEKIVPVMAVLYVLGSLVVLAVNYRA
                     VPGAFAQIFVMAFNPKAMLGGVLGVTVQQAVRYGVARGLFSNEAGMGSTPHAHALAKV
                     EHPCEQGVVAMIGVFIDTFVVLTMTALVILTTGALGTGATGAPLAQNAFNSAFGAFGD
                     IFIAVCMLFFAFSTIVGWYFFGETNVKYLFGKKAVKWYTALVLVFIVLGSLAKVQLVW
                     DLSDLFNGLMVIPNLIAVVALGGVVAAKYREYAAREKRKKERELSEKEG"
BASE COUNT          633 a         1028 c          820 g          644 t
ORIGIN      
        1 ttgatccagt ccgcccccgc gcgcagctgc tcacgggcgg ctttgcggca gtcgtccacc
       61 ccgtccgcct cgcggcccat catccagccg tggccgccgg tcatacagat gcatttgccg
      121 gccacctgca tgtccggcgc gaccagcttg ccctttttcg cctcgttgcg gatatcaata
      181 tcgatgtaat tcatgccgcc cacatcgcgg ataaacgtac agccgctgtc cacgaacttt
      241 ttcaggtttt ccagcgccat catggtaatc tggcagtcgt tcttgtcgta tttcgggtcg
      301 gcgtccggcg ccaggcacat atgggtgtga cagttgaaca ggcccggcat cagccatttg
      361 ccgcccaggt ccaccacctc ggccccctgc gggcaggtca gttccgcgcc cacctccgtg
      421 atctggctgc cttccaccac aacagacccg gcaaagactt tctcattgac gacgtccaca
      481 atgttacaat ttgtaaaaaa ctttgtgctc atgaactttc ctcccactta aacatttttc
      541 aaacctgtcg atgcgcatgt ttgatttgtg ccattatagc ttttttgacg cggttttccc
      601 atatccgttt tttccaaact ttttcaataa cgctgcgatt tggttatggg gtttatgaaa
      661 aatgcacaat gagaaaaaaa atcgcctcta attctgtaac tatgttgtaa catttatgct
      721 gtgttctctc gcaagcttat tcttttgtcc aaaggcggca ctgccgcaga cggggcgccg
      781 ggtcatatcc ctgatcggcc cccatggagg aaagaacccc gcagggaaac tctgggcaaa
      841 ggccgcagtg gtcgagcccc ctgccctcgc agcaggactt caccgggcac tcgccgcccc
      901 agaaaggcaa tgccatggaa aggcagcctg tgcagtggac ctccgcctta cgctcacagt
      961 cgttgcagca gatgccgcat ctcgattcaa acatgggtac actcctcctc gtcgttcctg
     1021 agaatttttt gttccgcgct ctgcgttttt tgctccacac tccgcacttt gcgccgcatg
     1081 ccgcggccgc ttcagcggat aaaattggtg cggctgccgc cctcctgctg cgggaaaacc
     1141 gcgccggcct gcgcacccgc ctcgatgcag cgcagcagcc aggccatgtt tcgcgccagg
     1201 ttgcgcatgg tctgcagccc ctcggcatcc cggcacgcct cctctgcggt gttgccgtgg
     1261 agcatgttcc aataggtgga ggacaccacc ggcatgccgc agatggtgaa atatttgttc
     1321 agctcgtcca gcgtcacagt ggtgcccgca cggcgcgcgg aggccaccgc cgccgcgggc
     1381 ttgcccgcaa agccgccgca ggcatagaac gcccggtcca gcaggctgac aaggctgccg
     1441 ttggcggacg cgtaatagac cggcgagccg accaccaggc cgtccgcctg cgccatcttt
     1501 tcgatcagca gatttgccgg gtcgtcgtcg aagacgcagc gtccctttcc cctgcagccg
     1561 ccgcaggcaa tgcagccccg caccggtttt gcgccgatcc acatcgtctc gctctccacg
     1621 ccgcattcgg ctagcgtttt cgccacctcg tccagcgcca ccgccgtgca gccgtgacgg
     1681 tgtgggctgc cgttgagcaa aagtacgttc atcttctccg ctccccttgc ctttttcgcc
     1741 agtgtaacac agctgtcgtc aaaagaaaag ggccgcgcgg cacaacccgc acaagccctt
     1801 atccaccgcc ggctctcagg gccggctttt gatcgctaac aatcttgccc ccctgttctt
     1861 gccgccgtgc ggcagctgga gcgggggacc ataaaaacgg ccatgccggc ccggcccacg
     1921 gccttgaatt tttgagtatc cgtcccctcc ctgggaggac ggctgaaaaa acagcctatc
     1981 ttgatggccg tggccaagga tgtgccgcgt ggccccaaaa tagcctgcgg ctatctttta
     2041 ttgctgcgca ataaaaaaca acttatttct atggccgcgt ttctcgccac tatcgcggca
     2101 accgaaacag atagccgaaa aagaatagcc gctgatcggt tattctttta cccctctttt
     2161 tcagataact cccgctcttt tttccgcttt tcacgggccg catattcccg atattttgcg
     2221 gccaccacgc cgcccagcgc caccacggcg atcaggttgg ggatgaccat cagcccgttg
     2281 aaaaggtccg acaggtccca caccagctgc accttggcca ggctgcccaa cacgataaaa
     2341 accagcacca gggcggtata ccatttgacc gcctttttgc cgaacaggta tttgacgttg
     2401 gtctcgccaa agaaatacca tcccacaatg gtcgaaaagg caaagaacag catgcagacg
     2461 gcgataaaga tatcgccgaa cgcgccgaac gccgaattga acgcgttttg agccagaggc
     2521 gcgccggtgg ccccggtgcc cagcgccccg gtggtgagga tgaccagcgc cgtcatggtg
     2581 agcaccacaa aggtgtcgat gaacacgccg atcatggcca ccacgccctg ctcgcagggg
     2641 tgctccacct tggccagcgc atgggcgtgg ggagtagagc ccatgcccgc ctcatttgaa
     2701 aacaaaccgc gcgccacgcc gtagcgcacc gcctgctgta ccgtgacgcc caacacgccg
     2761 cccagcatgg ccttggggtt aaacgccatg acaaagatct gggcaaaggc gcccgggacg
     2821 gcgcgataat tgaccgccag taccaccagg ctgcccagca cgtacagcac cgccatcacc
     2881 ggcacgatct tttccgtcac tgaggcgatg cggcgcttgc cgccgagaaa gatgaacgcc
     2941 gcagccgcgg cgaccagcac ccccatcaac agcggcgggg tgtgaaacgc cttttgaaag
     3001 gcgtcgctga tcgagttgga ctgtaccatg ttgcccataa agcccagggc cacgatgatc
     3061 gccaccgaaa agaagcccgc cagaaagctg ccgaaccggc ccttgaaccg cgcttttatg
     3121 tagta
//
LOCUS       DBMDYA010000315         3125 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6973_length_3125_cov_1.139220, whole genome shotgun sequence.
ACCESSION   DBMDYA010000315 DBMDYA010000000
VERSION     DBMDYA010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_3125_cov_1.139220"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="ACZPFK_08250"
     CDS             <1..604
                     /locus_tag="ACZPFK_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1406341.1"
                     /translation="RYGPLRPVGLVNPATGHRPWANVQLRSEDRANSRYNLVGFQTNL
                     RFGEQKRVFSMIPGLEQAEFVRYGVMHRNSFLNGPQVLGADLSLKNWPRLYFAGQITG
                     VEGYMESAACGLLAAHQVLRRLRGLPPLVLPPETMCGALMAHVTTETADYQPMGSNMG
                     LLPPLPERVKDKRLRYQQLAERAVAAMQQALLSQGALTAR"
     gene            682..1671
                     /gene="plsX"
                     /locus_tag="ACZPFK_08255"
     CDS             682..1671
                     /gene="plsX"
                     /locus_tag="ACZPFK_08255"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR1406342.1"
                     /translation="MKIILDAMGGDNAPKELLLGAAAAVKELGVEIIAVGDEEKIGAC
                     ARENGIEMAGIAVKHAAETIEMCDDPTSAVRHKKDSSMVVALRLLAAGEGDALVSAGS
                     TGALLTGATLIAKRLPGVKRAAIGTVIPGQQQPYLLLDSGANVECPAEMLDCFATMGS
                     VYMEKVMGRREPKVALVNNGAEESKGTPVYVQAHGLMKQNPAIRFAGNVEPRDVPTGA
                     VDVVVCDGFTGNVILKLTEGLAKFFTGELKAMFKKNAGTMFAALLMKNGVREFKAKLD
                     ADEHGGAPLLGISRVVIKAHGSSNAKAIKNAVRQAKLCCENGMVEAIRENIKR"
     gene            1753..2436
                     /gene="rnc"
                     /locus_tag="ACZPFK_08260"
     CDS             1753..2436
                     /gene="rnc"
                     /locus_tag="ACZPFK_08260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1406343.1"
                     /translation="MENLQGLERALGHQFSNIKLLAAAVTHSSFANEQRRGTEYNERL
                     EFLGDSVLSIVVSEDLFTSRRHLPEGELTRIRAALVCETSCYEFAKKIGLGQYLRLGR
                     GEENSGGRTRPSILADAFEAVLAALYLDGGLEAARAFILPLVRDEQAVPEDYKTRLQE
                     IIQQNPEERLRYVVVDESGPDHNKSFVVEVHLNSNVIGRGEGHSKKLAEQHAAREALK
                     LFGEGGGRL"
     gene            2433..>3125
                     /locus_tag="ACZPFK_08265"
     CDS             2433..>3125
                     /locus_tag="ACZPFK_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HIR1406344.1"
                     /translation="MTPRHRNLAFFVPHAGCPHRCSFCDQNSISGAAAGPSPEEVAER
                     CDALLPGPGEGRQVEIAFFGGSFTAVDAAYRQALLAAAAPFVEAGRAFGIRVSTRPDA
                     IDPAILAELRRWGVSAIELGAQSMNDTVLRLNGRGHTALQTKFAAQLVKNSGFSLGLQ
                     MMVGLFGEYDPARAARDTAIALANLGPDTVRIYPTLVLRGTPLAELTRRGSYRPLGVE
                     QAVVATAPLIELF"
BASE COUNT          542 a          997 c         1133 g          453 t
ORIGIN      
        1 gcgctatggc ccgctgcggc cggtggggct ggtgaacccg gccaccggcc accgcccctg
       61 ggcaaacgtg cagctgcgca gcgaggaccg ggcgaacagc cgctacaatc tggtgggctt
      121 ccagacgaac ctgaggttcg gcgagcagaa gcgggtcttt tcgatgatcc ccggcctgga
      181 gcaggcggag ttcgtgcgct acggcgtgat gcaccgcaac agctttctca acgggcccca
      241 ggtgctgggg gcggacctgt cgctgaaaaa ctggccccgc ctttactttg cggggcagat
      301 caccggggtg gagggctata tggagagcgc ggcctgcggc ctgctggccg cccatcaggt
      361 gctgcgcagg ctgcggggcc tgccgccgct ggtgctgccg cccgagacca tgtgcggcgc
      421 cctgatggcc catgtgacga ctgagacggc ggattaccag cccatgggca gtaatatggg
      481 cctgctgccg cccctgcccg agcgggtgaa agacaagcgg ctgcgctatc agcagctggc
      541 cgagcgggcg gtcgcggcta tgcagcaggc cctgctcagc cagggcgcgc tgacggcgcg
      601 ctgagagagg caaaaacagc aaggggcggg cagggggcca tcattgtgcc gcccgcccgc
      661 acaaaagggg aggatggaag catgaagatc attttggacg ccatgggcgg ggacaacgcg
      721 ccgaaagaac tgttgctggg cgcggccgcg gcggtgaaag agctgggcgt ggagatcatc
      781 gccgtgggcg atgaggagaa gatcggcgcc tgcgcgcggg aaaacggcat cgagatggcg
      841 ggcatcgcgg tgaagcacgc cgccgagacc atcgagatgt gcgacgaccc cacctctgcg
      901 gtgcggcaca aaaaggattc gtcgatggtg gtggcgctgc ggctgctggc cgcgggcgag
      961 ggggacgcgc tggtcagcgc cggttccacc ggggccctgc tcaccggcgc gacgctgatc
     1021 gccaagcggc tgccgggcgt caagcgggcg gccatcggca ccgtcatccc cggccagcag
     1081 cagccctacc tgctgctgga ttcgggcgcg aacgtggagt gccccgccga aatgctggac
     1141 tgctttgcca ccatgggcag cgtgtacatg gaaaaggtga tgggccgccg ggagccgaag
     1201 gtggccctgg tcaacaacgg cgccgaggag agcaagggca ccccggttta cgtgcaggcg
     1261 cacgggctca tgaagcagaa ccccgccatc cgttttgcgg gcaacgtgga gccgcgcgac
     1321 gtgcccaccg gcgcggtgga cgtggtggtc tgcgacggct ttaccggcaa cgtcatcctc
     1381 aagctcaccg agggcctggc caagtttttt accggcgagc tgaaagccat gttcaagaaa
     1441 aacgccggca ccatgttcgc ggccctgctg atgaaaaacg gcgtgcgcga attcaaggcc
     1501 aagctggacg ccgacgagca cggcggcgcg ccgctgctgg gcatcagccg ggtggtcatc
     1561 aaggcccacg gctcgtccaa cgccaaggcc atcaaaaacg cggtgcgcca ggcaaagctc
     1621 tgctgtgaga acggcatggt ggaggcgatc cgcgagaaca tcaagcggta acataagcgg
     1681 tttgggccgg ggcgcagcag ccccggcggc gccggcaagg cgcttttccc ggggaaagga
     1741 agatgcggtt ccatggaaaa tctacagggg ctcgagcggg cgctcggcca tcaattcagc
     1801 aacatcaagc tgctggcggc ggcggtcacc cactccagct ttgccaacga gcagcggcgc
     1861 ggcaccgagt acaacgagcg gctcgagttc ctgggcgact cggtgctctc cattgtggtc
     1921 agcgaggacc tcttcacctc ccgccggcat ctgccggagg gcgagctcac ccgcatccgg
     1981 gcggcgctgg tctgcgagac cagctgctat gagttcgcaa aaaagatcgg tctcgggcaa
     2041 tatctgcgcc tgggccgggg cgaggagaac agcggcgggc gcacccgccc ctccatcctg
     2101 gcggacgcct ttgaggcggt gctggcggcg ctgtatctgg acggcgggct ggaggcggcg
     2161 cgcgcgttca tcctgccgct ggtgcgggac gagcaggcgg tgccggagga ttacaagacc
     2221 cgcctgcagg agatcattca gcaaaacccc gaagagcggc tgcgctatgt ggtggtggat
     2281 gagtccggcc cggaccacaa caagagcttt gtggtagagg tgcacctgaa ctccaacgtc
     2341 atcgggcggg gcgagggcca cagcaaaaag ctggccgagc agcacgccgc cagggaggcg
     2401 ctgaagctgt tcggcgaggg gggcgggcgg ctgtgacgcc ccgccaccgc aacctggcct
     2461 tttttgtgcc ccacgcgggc tgtccccacc gctgcagctt ctgcgaccag aactccatct
     2521 ccggcgcggc ggcggggccg tcccccgagg aggtcgccga aagatgcgat gccctgctgc
     2581 ctggccccgg agaggggcgg caggtcgaga tcgccttttt cggcggcagc ttcaccgccg
     2641 tcgacgccgc ctaccggcag gccctgctgg ccgcggccgc cccctttgtc gaggcggggc
     2701 gcgctttcgg catccgggtc tccacccggc ccgacgccat cgaccccgcc atcctcgccg
     2761 agctgcgccg ctggggcgtg agcgcgatag agctgggggc ccagagcatg aatgacaccg
     2821 tgctgcggct caacggccgc gggcacaccg ccctgcagac caagtttgcg gcccagctgg
     2881 tgaagaacag cggtttttcg ctggggctgc agatgatggt gggcctgttc ggagaatacg
     2941 acccggcccg cgcggcgcgg gacaccgcca tcgcgctggc gaacctcggc ccggacacgg
     3001 tgcgcatcta ccccacactg gtgctgcggg gcacgccgct ggccgagctg acgcggcggg
     3061 gcagctaccg cccgctgggg gtggaacagg cggtggtggc caccgcgccg ctgatcgagc
     3121 tgttt
//
LOCUS       DBMDYA010000316         3118 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_6991_length_3118_cov_1.581372, whole genome shotgun sequence.
ACCESSION   DBMDYA010000316 DBMDYA010000000
VERSION     DBMDYA010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_3118_cov_1.581372"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="ACZPFK_08270"
     CDS             complement(<1..639)
                     /locus_tag="ACZPFK_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1406345.1"
                     /translation="MRIEGLQKLTLLDYPGHLACTVFLGGCNLRCPFCHNADLVLPGR
                     PSAALSREELTAFLKKRRGVLQGVCVTGGEPLASQGVEELFREIKSLGYAAKLDTNGT
                     FPDRLQGLVAQGLVDYVAMDVKNAPASYGATVGLPGFDPAPVLRSAAFLLSGAVEYEF
                     RTTVVDELHTEADFEAIGRWLAGAKRYFLQGFVDSGNLVGQGMHAASKERMER"
     gene            complement(997..2016)
                     /locus_tag="ACZPFK_08275"
     CDS             complement(997..2016)
                     /locus_tag="ACZPFK_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406346.1"
                     /translation="MATIKELAAYTGLSPSTVSIVLGGKAQQRKIPESTQRRVQEAAQ
                     ALGYRPNISARRLRDPEAKESMVVAVFWTEDFRAPMMVRFLRGLRRAIEAQGGGCEVL
                     IHPYTCGRLREERSLISLSMFNAAIICNASEEDLAWLEQAKPLLPIVLYNRHSEGYCT
                     VNVDDARIGALPAEVFSAHGCRRAAVLTADEVFYGMRVRTDSFLARCGELGLAAGPVL
                     RQENSMRGGCEGAQLLAALGPAHRPDCLFCASDALALGALRGFHAAGVELPGQMEIIS
                     VGNGDRDARQYAWPSLSAVSVPIEEMATECFYLLRRLVRGEVKPPFSRSMEVRYLPGE
                     SCPEG"
     gene            2198..>3118
                     /locus_tag="ACZPFK_08280"
     CDS             2198..>3118
                     /locus_tag="ACZPFK_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR1406347.1"
                     /translation="MKKVAIGGGQGFWGDSNDAAVHLLRHGDIQYLGCDYLAELTLSI
                     MARQKLKNPAAGYARDFIGLMREAGADAYRRGIRVVTNAGGMNISGAVEALRAEMEAQ
                     GLHGYRIGYVLGDDVKDRIPELLAQGVEFKNLDDVGDFAEVRDQVFNANVYYGHEAIA
                     DCLAQGADTVLCGRAADSAMFLAPFVHEFGWAADDWDNLARGIMAGHLLECGGQGSGG
                     NYQYGWREVPEMDDLGFPIAEITPDSFEITKAENCGGLICEQSCKEQFLYEVHDPANY
                     LTPDVNVDISRATLTQAGRDRVRVGGVRGKP"
BASE COUNT          547 a         1081 c         1016 g          474 t
ORIGIN      
        1 ccgctccatc cgctcttttg aggcggcgtg catcccctgg cccaccaggt tgcccgagtc
       61 cacaaacccc tgcaaaaagt agcgcttcgc ccccgccagc cagcggccga tggcctcaaa
      121 atccgcctcg gtgtgcagct cgtccaccac ggtggtgcga aactcatatt ccaccgcgcc
      181 cgagagcaaa aacgcggcgc tgcgcagcac cggcgcgggg tcaaagccgg gcaggcccac
      241 ggtggcgccg tagctcgccg gcgcgttttt cacatccatg gccacatagt ccaccaggcc
      301 ctgcgccacc agaccctgca gccggtcggg aaaggtgccg ttggtgtcca gcttggccgc
      361 gtagcccagg ctcttgatct cccggaacag ctcctccacg ccctgcgacg ccagcggctc
      421 gccgccggtg acgcagaccc cctgcaaaac gccgcgccgc ttttttaaaa aggcggtcag
      481 ctcctcgcgg ctgagggcgg ccgaggggcg gcccggcagc accaggtcgg cgttgtggca
      541 aaacgggcag cgcaggttgc agccgccgag aaaaacggtg catgccagat gcccgggata
      601 atccagcagg gtcaactttt gcagtccctc gatcctcata acagctccct ttccggcgcg
      661 gggccgctct gcggccggcc gcgcactgaa gcgtgcggcg caggcgcagc ctgcgcttgc
      721 accgcacgct ctctcttatc ttttattctt ctatctttta ttcttcctcc gggggccgcc
      781 ccaaggcgct cagacggcgg ctcttcgtcc ccaaaaccgg gccgccgctg cggcgggagc
      841 gggtgcgcgc cgcggctccc gcttttgcgc gccgccgacc tgccgttgcg gcgggcgaag
      901 cggcggcttt gaccgggcgg tgggagaacg gacgctcccc gcgggaccgg cgctacggcg
      961 ggcgaagcgg cggcctgcaa ggacgaaaag ccgcgcctag ccctccgggc agctctcgcc
     1021 cggcaggtag cgcacctcca tcgagcggga aaagggcggc ttcacctcgc cccgcaccag
     1081 ccgccgcaac aggtaaaagc actcggtcgc catctcctcg atgggcacgc tcaccgccga
     1141 caggctgggc caggcatatt gccgggcgtc ccggtcgccg ttgcccaccg agatgatctc
     1201 catctggccc ggcagctcca ccccggcggc gtgaaagccg cgcagcgccc ccagcgccag
     1261 cgcgtccgag gcgcagaaca ggcagtccgg cctgtgggcc gggcccagcg ccgccagcag
     1321 ctgcgcgccc tcgcagccgc cccgcatcga attttcctgt cgcagcaccg gcccggcggc
     1381 aagccccagc tcgccgcagc gggccaaaaa gctgtcggtg cgcacccgca tgccgtagaa
     1441 cacctcgtcc gccgtcagca ccgccgcgcg gcggcagccg tgggcggaaa agacctccgc
     1501 cggcagcgcg ccgatgcggg cgtcgtcaac attgaccgtg caatagccct cgctgtgccg
     1561 gttgtacagc acgatgggca gcagcggctt tgcctgctcc agccaggcca ggtcctcctc
     1621 ggaggcgttg cagatgatcg cggcgttgaa catcgagagg gagatgaggg accgctcctc
     1681 ccgcagcctg ccgcaggtat aggggtggat cagcacctca cagccgccgc cctgcgcctc
     1741 gatggcacgc cgcagcccgc gcagaaagcg caccatcatc ggggcgcgga aatcctccgt
     1801 ccaaaagacc gccaccacca tgctctcctt ggcctcgggg tcgcgcaggc ggcgggcgga
     1861 aatgttgggc cggtagccca gggcctgcgc cgcctcctgc acccgccgct gggtgctctc
     1921 ggggatcttg cgctgctgcg ccttgccgcc cagcacaatg gacacggtgc tgggggaaag
     1981 gccggtatag gcggccagct ctttgatggt ggccataaaa gctcccctcc ctgctgtcaa
     2041 tccggcaaaa caactcaaac gctctactta aattatagaa tacttcacaa aaattgcaag
     2101 cggcagttga cagcaccgcg gggacggggt atgctgaaac cacgttttaa gtacaacgta
     2161 atacttaaat tccaaatctt ctttcaggag gctgcacatg aaaaaagttg ccatcggggg
     2221 cggccagggg ttctggggcg actccaacga cgcggcggtg catctgctgc gccacgggga
     2281 catccagtat ctgggctgcg actatctggc cgagctgacc ctctccatca tggcgaggca
     2341 aaaactgaaa aatcccgccg caggctatgc ccgcgacttc atcggcctga tgcgggaggc
     2401 gggggcggac gcctaccggc gcggcatccg cgtcgtcacc aacgccggcg gcatgaacat
     2461 cagcggcgcg gtggaggcgc tgcgggccga gatggaggcc cagggcctgc acggctaccg
     2521 catcggctac gtgctgggcg acgacgtgaa agaccgcatc cccgagctgc tggcccaggg
     2581 ggtggagttc aaaaatctcg acgacgtggg cgatttcgcc gaggtgcggg accaggtctt
     2641 caacgccaac gtctactatg gccacgaggc catcgcggac tgcctggccc agggggcgga
     2701 cacggtgctc tgcggccgcg cggcggacag cgccatgttt ttggcgccct ttgtgcacga
     2761 gttcggctgg gcggcggacg actgggacaa tctcgcccgg ggcatcatgg ccggccatct
     2821 gctggagtgc ggcggccagg gctcgggcgg caactaccag tacggctggc gggaggtgcc
     2881 ggagatggac gatctgggct ttcccatcgc agagatcacc cctgacagct ttgagatcac
     2941 caaggcggag aactgcggcg gcctgatctg cgagcagagc tgcaaggagc agttcctcta
     3001 cgaggtgcac gacccggcca actatctgac cccggacgtg aacgtggaca tcagccgcgc
     3061 caccctgacc caggccgggc gcgaccgggt gcgggtgggc ggcgtgcgcg gaaagccc
//
LOCUS       DBMDYA010000317         3112 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7005_length_3112_cov_1.701428, whole genome shotgun sequence.
ACCESSION   DBMDYA010000317 DBMDYA010000000
VERSION     DBMDYA010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_3112_cov_1.701428"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="ACZPFK_08285"
     CDS             complement(<1..396)
                     /locus_tag="ACZPFK_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1406348.1"
                     /translation="MRERYLLSIDQSTQGTKAMLFSGAGGLLCRCDRKHRQIVSEQGW
                     VEHDPMEIYQNTLAVVGELLQKSGVDPASVAGAGLSNQRETVLVWDKKTGLPAYNAVV
                     WQCGRATEQCRRIAGQGRRIQQITGLPLSP"
     gene            complement(393..1328)
                     /locus_tag="ACZPFK_08290"
     CDS             complement(393..1328)
                     /locus_tag="ACZPFK_08290"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1406349.1"
                     /translation="MSVPANKQVICEVLKEAVRRDPNIVVLCSDSRGSSSMTSFFEEY
                     PDNSVEVGIAEQDLVTISAGLAASGKRPFAVSPASFLAARSFEQIKIDVGYSRTNVKL
                     VGISGGVSYGALGSSHHSAQDIAAVGALPGLRVYLPSDWRLTRALTEQLARDNEAAYV
                     RVGRSASPEVYGEGELRFQMDRAVTVREGGDLAVIACGDAVYEAVCAADRLAAEGIRC
                     RVLDMYCLKPLDTEAVLRAARETGRILTVEEHSIVGGLGSLVCQATARACPVPVQSLA
                     LPDSEIVTGSQRQVYHYYGLDAEGICRAARKMVAL"
     gene            complement(1309..2145)
                     /locus_tag="ACZPFK_08295"
     CDS             complement(1309..2145)
                     /locus_tag="ACZPFK_08295"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1406350.1"
                     /translation="MRLERKAVELRLEILDLICSAGTGHIGGDFSAIDILTYLYYEGM
                     NISPATVDDPDRDRFILSKGHAVEALYSVLADRGFFDRQALHETYSQLGSPFIGHPNN
                     QLPGIEMNSGSLGHGLPAAVGIALAGQMDHRRYYTYVLTGDGELAEGSVWEAFMAAPH
                     FGLDHLVAIIDRNGLQISGSTEDVMPQGDLAGKLKLFGWDVQEIDGNSIPAIRAAVQR
                     AKQTGGRPTAIIANTVKGCGVSFMQNRAGWHHRVPTAEEAALARDELMQKARELDERS
                     GE"
     gene            2424..>3112
                     /locus_tag="ACZPFK_08300"
     CDS             2424..>3112
                     /locus_tag="ACZPFK_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406351.1"
                     /translation="MSVSAKSIARELGLSQSTVSLALRNLPGISDSTRRRVLEKAAEM
                     GYQKSALAPAEAPRFLSLVLYKKHGSVLADTPFFSDLIQGIDQQAKKCGYQVFITYFY
                     ENQNIQEQLESLKKSLCSGIILLATEMHDRDFLAFQSLEIPLVALDRYFPEFDCDCVV
                     INNIYGAKKAVQHLIEKGHTRIGYLRSSVEIRNFRERFEGYSKGIHLLPHPEESRPFI
                     VRISPVAEEAVG"
BASE COUNT          583 a         1016 c          921 g          592 t
ORIGIN      
        1 gggggaaagg ggcagcccgg tgatctgctg gatgcgccga ccctggccgg cgatccgccg
       61 gcactgctcg gtcgcccggc cgcactgcca gacgacggcg ttgtaggcgg gcaggccggt
      121 ctttttgtcc cagaccagca ccgtctcccg ctggttgctg aggcccgcgc ccgccaccga
      181 ggccgggtcc acgccgctct tttgcagcag ctcgcccacc acggccagcg tgttttggta
      241 aatctccatc gggtcgtgct ccacccagcc ctgctcggag acgatctggc ggtgcttgcg
      301 gtcgcagcgg cagagaagac cgcccgcccc gctgaacagc atcgccttgg tgccctgggt
      361 gctctggtcg atggagagca gatatctttc tctcacagcg ccaccatctt tcgcgcggcc
      421 cggcagatgc cctcggcgtc caggccgtag tagtggtaga cctggcgctg gctgccggtg
      481 acgatctcgc tgtccggcag ggcgaggctc tgcaccggca ccgggcaggc ccgcgccgtg
      541 gcctggcaga ccagcgagcc cagcccgccc acgatgctgt gctcctccac ggtgaggatg
      601 cggccggtct cccgcgccgc ccgcagcacc gcctcggtgt ccagcggctt gaggcagtac
      661 atgtccagca cccggcagcg gatgccctct gcagccagcc ggtcggcggc gcacacggcc
      721 tcgtacaccg cgtcgccgca ggcgatgacg gcgaggtcgc cgccctcgcg gacggtcacg
      781 gcccggtcca tctggaaccg cagctccccc tcgccgtaca cctcggggct ggcgctgcgg
      841 cccacccgca cataggcggc ctcattgtcc cgcgccagct gctccgtgag ggcgcgggtc
      901 agccgccagt cgctgggcag atagacccgc aggccgggca gcgcgcccac cgccgcgatg
      961 tcctgggcgg agtggtggct ggagcccagc gccccgtagc tgaccccgcc cgagatgccc
     1021 accagcttga cgttggtgcg ggagtagccc acgtcgatct tgatctgctc aaagctgcgg
     1081 gccgccagga agctggcggg tgaaacggca aatggccgct tgccgcttgc cgccaggcca
     1141 gcgctgatgg tcaccaggtc ctgctcggca atgccgacct ccacgctgtt gtccgggtat
     1201 tcctcaaaaa aggaggtcat cgagctggac ccccgcgagt cgctgcagag caccacaatg
     1261 ttcgggtccc gccgcaccgc ctctttcagc acctcgcaga tcacctgctt attcgccgga
     1321 acgctcatcc aattccctcg ctttctgcat cagctcgtcg cgggccagcg ccgcctcttc
     1381 agcggtgggc acccggtgat gccacccggc cctgttctgc ataaaggaga cgccgcagcc
     1441 tttgacggtg tttgcaatga tcgccgtggg gcggccgccc gtttgcttgg cccgctgcac
     1501 ggcggcccgg atggccggga tgctgttgcc gtcgatctcc tgcacgtccc acccgaagag
     1561 cttcagcttg cccgccaggt cgccctgggg catcacgtcc tcggtgctgc cgctgatctg
     1621 cagcccgttg cggtcgatga tggccaccag atggtccagg ccaaagtggg gggcggccat
     1681 gaaggcctcc cacaccgagc cctcggccag ctcgccgtcg ccggtgagca cataagtata
     1741 gtagcggcga tggtccattt gcccggcgag ggcgatgccc accgcggcgg gcagcccgtg
     1801 ccccagcgag ccggagttca tctcgatgcc cggcagctgg ttgttggggt gaccgataaa
     1861 ggggctgccc agctgggaat aggtctcgtg cagcgcctgc cggtcaaaga acccccgatc
     1921 cgccagcacg ctgtagagcg cctccaccgc gtgccccttg ctgaggatga accggtcccg
     1981 gtccgggtcg tccacggtgg cgggcgagat gttcatgccc tcatagtaga gataggtgag
     2041 gatatcaatg gcgctgaagt cgccgccgat gtggccggtg ccggcgctgc agatgaggtc
     2101 caggatctcc agccgcagct ccacggcttt tctttccagt ctcaagggac tccctccttt
     2161 tcaaaaattg aataaaaagt tttcaataaa atctaaataa tttttgttta caatcgaatt
     2221 atatttctaa aatttagaaa aatcaaccta aaatacaatt tttattaaga ttacacataa
     2281 aaattaagta tttttatagc atagaaacca ataaatggac aaaaagaaaa aataatttag
     2341 aaaattattt atttaattat tgacaaaccc attttattcc tttatcttta agttgagcga
     2401 atatgaaaat ttggaggcca cttatgtctg tatcggcaaa atccatcgcc agagaactgg
     2461 ggctctccca gtccacagtg tccctcgcgc tgcgcaacct gccggggatc agcgactcca
     2521 cccgccgcag ggtgctggaa aaggcggcgg agatggggta tcagaaaagc gccctcgcgc
     2581 ccgcggaggc gccccgcttc ctctccctgg tgctctataa aaagcacggc agcgtgctgg
     2641 ccgacacgcc ttttttctcc gacctgatcc agggcatcga ccaacaggcg aaaaagtgcg
     2701 gctatcaggt cttcatcacc tatttctatg aaaaccagaa tatccaggag cagctggagt
     2761 ccctcaaaaa atccctctgc tcgggcatca tcctgctggc caccgagatg cacgaccggg
     2821 actttttggc ctttcagtcg ctggagatcc cgctggtggc gctggaccgc tatttccccg
     2881 agttcgactg cgactgtgtg gtcatcaaca acatctacgg cgcaaaaaaa gcggtgcagc
     2941 acctgatcga aaaaggccac acccgcatcg gctatctgcg ctccagcgtg gagatccgca
     3001 atttcagaga gcggttcgag ggctacagca agggcatcca cctgttgccc cacccggagg
     3061 agtcccgccc gttcatcgtg cgcatctccc cggtggcaga ggaggcggta gg
//
LOCUS       DBMDYA010000318         3112 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7006_length_3112_cov_1.622385, whole genome shotgun sequence.
ACCESSION   DBMDYA010000318 DBMDYA010000000
VERSION     DBMDYA010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_3112_cov_1.622385"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="truB"
                     /locus_tag="ACZPFK_08305"
     CDS             complement(<1..858)
                     /gene="truB"
                     /locus_tag="ACZPFK_08305"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1406352.1"
                     /translation="MNGILIVDKPAGWTSFDVIAKLRGALHTKKIGHSGTLDPLATGV
                     LPLFVGGAVKAVDLPQNHRKSYLATLRFGVHTPTGDLESAPDELRPGRVERAALEEVL
                     PGLVGPGEQLPPMYSAVKVNGQPLYKAARAGREVERRPRPIEIFSLQCAGPGPGENEW
                     QVAVSCSKGTYIRVLVEEIANRLSTVAVMSALRRTASGDFALADAHPMGTILEAAEKG
                     EAQRLLLPVDSVFGQLPRLDASEELASRLWHGAPTHVRLADGLYRVYAQGAFLGLASA
                     EQGVVRVKKL"
     gene            complement(855..1805)
                     /locus_tag="ACZPFK_08310"
     CDS             complement(855..1805)
                     /locus_tag="ACZPFK_08310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1406353.1"
                     /translation="MTERIDLAAVMERLAGAQDILLLCHKNPDGDTLGSAGALYWALT
                     AQGKRAAIFCSDPVTDRYDYMQLGLYDGSWEPRYVVAIDVAGIQLFGEGASQYLKRID
                     LCIDHHPSNSGYADATLLEPEAAATAEIIYLLLCRMEVEFTPVMADCLYTGVSTDTGC
                     FKFANTTPRTHHIAAELMERGAHFVQLNELLFESKSQRRLAIEQTALSHLEYHFDGRC
                     ALMYVSRDEILATGADSNDLEGITSIPRMIEGVVVGVTMRQLESGSYKVSVRTAGEID
                     ACKICAALGGGGHMRAAGCEFFGNLENAKAAVLAEVEKQI"
     gene            complement(1798..2145)
                     /gene="rbfA"
                     /locus_tag="ACZPFK_08315"
     CDS             complement(1798..2145)
                     /gene="rbfA"
                     /locus_tag="ACZPFK_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1406354.1"
                     /translation="MAQSNHMGRLAEDMKRELIDIISHMKDPRVNGLLTVMRVEVTPD
                     LDTAKVYISVLGEEDAPKKAVEALNHAAGHVRSEIAKRMHIRKSPSFRFIEDDGAAYA
                     AHINKILGELHHD"
     gene            complement(2215..>3112)
                     /locus_tag="ACZPFK_08320"
     CDS             complement(2215..>3112)
                     /locus_tag="ACZPFK_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1406355.1"
                     /translation="TLKKGDCIIAGTAVGRVRVMRNDKGEPIETAGPSTPVEITGLTE
                     VPAAGDLFEAVADEKLARELADQRAAKQKEEQWGSYQKVTLDNLFSQIEEGERKELPI
                     VVKADVQGSAEAVKQSLEKISNDEVRVKVIHAGVGGISKSDVMLAAASGAIIIGFNVR
                     PDPVAKAEAAQAEVELRMYRVIYDAINDVTDAMKGLLAPKIREVEQGRIEVRNVFKIS
                     SAGTIAGSYVLDGKITRGSKIRLVRDGVVITEDEIGSLRRFKDDVKEVAAGYECGIGL
                     EKYNDIHEGDIFEAYILEEYRD"
BASE COUNT          472 a         1068 c          986 g          586 t
ORIGIN      
        1 cagctttttc acccgcacaa cgccctgctc cgccgacgcc aggcccaaaa acgcaccctg
       61 cgcgtagacg cggtaaaggc cgtccgccag gcgcacatgg gtgggcgcgc cgtgccagag
      121 gcgggaggcc agctcctcag aggcgtccag gcgcggcagc tggccaaaga cgctgtccac
      181 cggcagcagc agccgctggg cctcgccctt ttcggccgcc tccaggatgg tccccatcgg
      241 gtgcgcgtcc gccagtgcaa aatcgccgct ggcggtgcgg cgcagcgcgc tcatcaccgc
      301 cacggtggaa aggcggtttg cgatctcctc caccagcacc cggatatagg tgcccttaga
      361 gcagctcacc gccacctgcc actcgttttc ccccgggccg gggccggcgc actgcagaga
      421 aaagatctcg atcggccggg gccggcgctc cacctcacgg cccgcgcgcg ccgccttgta
      481 gagcggctgc ccgttgacct tgaccgccga atacatgggg ggcagctgct cgcccgggcc
      541 gacgaggccg ggcagcacct cctccagcgc cgcccgctcc acccggccgg gacgcagctc
      601 gtccggtgcg ctctccagat cgccggtggg ggtgtgcacc ccaaagcgca gcgtcgccag
      661 atagcttttg cggtggttct ggggcaggtc caccgccttg acggcgcccc ccacaaaaag
      721 cgggagcacg ccggtcgcaa gcggatcgag cgtgcccgag tggccgatct ttttcgtgtg
      781 cagcgcgccg cgcagcttag cgatcacgtc aaagctggtc cagcccgccg gtttatccac
      841 gatcaggatg ccgttcatat ctgtttttcc acctcggcca gcaccgccgc cttggcgttc
      901 tccaggttgc cgaagaactc gcagccggcg gcccgcatat ggccgccgcc gcccagcgcg
      961 gcgcagatct tgcaggcgtc gatctcgccc gcggtgcgca cgctgacctt gtagctgccg
     1021 ctttcgagct ggcgcatggt cacgcccacc acgaccccct cgatcatgcg cgggatgctg
     1081 gtgatgccct ccaggtcgtt gctgtccgcc ccggtcgcca aaatctcgtc gcgggagacg
     1141 tacatcagcg cgcagcgccc gtcaaaatga tattccagat gggagagcgc cgtctgctca
     1201 atggcgagac gccgctggct cttgctctca aacagcagct cattgagctg cacaaaatgc
     1261 gcgccccgct ccatgagctc cgccgcgatg tggtgggtgc gcggggtggt gttggcaaac
     1321 ttgaagcagc cggtgtcggt ggacacgccg gtgtacaggc agtccgccat gaccggggtg
     1381 aactcgacct ccatgcggca gagcagcaga tagatgatct ccgccgtggc ggcggcctcc
     1441 ggctccagca gggtggcgtc ggcgtagccc gagttgctgg ggtggtggtc gatgcacagg
     1501 tcgatgcgct tgagatactg cgaggccccc tcgccgaaca gctggatgcc agccacgtcg
     1561 atggccacca cgtaccgcgg ctcccagctg ccgtcataaa ggcccagctg catataatcg
     1621 taccggtctg tgacggggtc gctgcagaag atcgcggccc gtttgccctg cgccgtcagc
     1681 gcccaataaa gcgcgccggc ggagcccaac gtgtcgccgt cggggttctt gtggcacagc
     1741 agcaggatgt cctgcgcccc ggcgagccgc tccatcaccg ctgccagatc gatgcgttca
     1801 gtcatggtgt aactccccta atattttgtt gatgtgggcg gcgtaggccg cgccgtcgtc
     1861 ctcgataaaa cgaaaggacg ggctcttgcg gatgtgcatc cgctttgcga tctcactgcg
     1921 cacatggccc gccgcatggt tcagggcctc caccgccttt ttgggggcgt cttcctcccc
     1981 cagaacgctg atatagacct ttgcggtgtc caggtcgggg gtgacctcca cccgcatgac
     2041 ggtcaaaagg ccgttcaccc gggggtcttt catgtggctg atgatgtcga tgagctcccg
     2101 cttcatgtcc tcggcgagac ggcccatgtg attggattgc gccatatcat ctctccttgc
     2161 cggcggacgg cgcgccgccg cccttttgtt catccgactt tatcctgccg cgattcagtc
     2221 gcggtattcc tcgaggatat aggcctcaaa gatatcgccc tcgtggatgt cgttgtattt
     2281 ttccagcccg atgccgcact cgtagcccgc ggccacctct ttgacgtcgt ccttaaagcg
     2341 gcgcagcgag ccgatctcgt cctcggtgat gacgacgccg tcgcgcacca ggcggatctt
     2401 gctgccgcgg gtgatcttgc cgtccagcac atagctgccc gcaatggtgc ccgccgagga
     2461 gatcttgaac acgttgcgca cctcgatgcg gccctgctcc acctcgcgga tcttgggggc
     2521 cagcaggcct ttcatggcgt cggtgacgtc gttgatggcg tcgtagatga cgcggtacat
     2581 gcgcagctcc acctcggcct gggcggcctc cgccttggcc acggggtccg ggcgcacgtt
     2641 gaagccgatg atgatggcgc ccgaagcggc ggccagcatg acgtcgctct ttgagatgcc
     2701 gcccacgccg gcgtggatga ccttgacccg cacctcgtcg ttggagatct tttccagcga
     2761 ctgcttgacg gcctcggccg agccctgcac gtcggccttg accacgatgg gcagctcttt
     2821 gcgctcgccc tcctcgatct ggctgaacag gttgtcgaga gtgaccttct ggtagctgcc
     2881 ccactgctcc tctttctgct tggcggcgcg ctggtcggcc agctcgcggg ccagcttttc
     2941 atccgccacg gcctcgaaca ggtcgcccgc ggcgggcacc tcggtgaggc cggtgatctc
     3001 gacgggggtg gaggggcccg cggtctcgat gggctcgccc ttgtcgttgc gcatcacgcg
     3061 cacacggccc acggcggtgc ccgcgatgat gcagtcgccc tttttcaggg tg
//
LOCUS       DBMDYA010000319         3109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7014_length_3109_cov_1.333444, whole genome shotgun sequence.
ACCESSION   DBMDYA010000319 DBMDYA010000000
VERSION     DBMDYA010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_3109_cov_1.333444"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            405..554
                     /gene="rpmG"
                     /locus_tag="ACZPFK_08325"
     CDS             405..554
                     /gene="rpmG"
                     /locus_tag="ACZPFK_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1406356.1"
                     /translation="MRVKITLACTECKQRNYNTMKNKKNSPDRLEMNKYCRFCKKHTL
                     HRETK"
     gene            570..845
                     /gene="secE"
                     /locus_tag="ACZPFK_08330"
     CDS             570..845
                     /gene="secE"
                     /locus_tag="ACZPFK_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1406357.1"
                     /translation="MAEKAEKAVKTEKPAKTEKKEKKPFFLVRFFRSIKKFFRDTFTE
                     MKKIVWPSKKQVINNTLVVIAVVILAGLVIFGLDNLFGLILKLILRT"
     gene            879..1397
                     /gene="nusG"
                     /locus_tag="ACZPFK_08335"
     CDS             879..1397
                     /gene="nusG"
                     /locus_tag="ACZPFK_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1406358.1"
                     /translation="MAEEGLWYVVHTYSGYENKVAQNLETLVENRRLHDLIQEVKVPT
                     EIVPEIKDGVKREVERKLFPGYVLVKMVMTDDSWYVVRNTRGVTGFVGPSSKPIPLTQ
                     AEVDALGVETHEAQVDYKVGDNVEVTAGPLEGFIGLVEEIDMEKQKVKVKVSMFGRET
                     SAELDLSQVKPL"
     gene            1524..1949
                     /gene="rplK"
                     /locus_tag="ACZPFK_08340"
     CDS             1524..1949
                     /gene="rplK"
                     /locus_tag="ACZPFK_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1406359.1"
                     /translation="MAQKVVGYIKLQIPAGKATPAPPVGPALGQHGVNIMSFTKEFNE
                     RTQKDMGYIIPVVITVYADRSFSFITKTPPAPVLIKKACGIESGSGAPNKTKVATITK
                     DQLREIAQTKMPDLNASSVESAMSMIAGTCRSMGVTVAE"
     gene            2010..2705
                     /gene="rplA"
                     /locus_tag="ACZPFK_08345"
     CDS             2010..2705
                     /gene="rplA"
                     /locus_tag="ACZPFK_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1406360.1"
                     /translation="MKHGKKYVDSAKLVDRTKTYEAEEALALCCQTAKAKFDETVELH
                     VRLGVDSRHADQQVRGAVVLPHGTGKDVRVIVIAKGDAAKAAEDAGAMMVGDEELAAK
                     IQNEGFIDFDVLITTPNMMGVVGRLGKILGPRGLMPNPKAGTVTMDVGRAVTEAKAGK
                     IEYRLDKANIIHVPIGKASFGAEKLGDNLKTVMDAIVKAKPAAAKGQYIKSATVASTM
                     GPGVKLNSARYGA"
     misc_feature    2739..2888
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2911..>3109
                     /gene="rplJ"
                     /locus_tag="ACZPFK_08350"
     CDS             2911..>3109
                     /gene="rplJ"
                     /locus_tag="ACZPFK_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1406361.1"
                     /translation="MPSEKILSQKQAYVKELEEKLKNSVAGVVVDYKGISVADDTKLR
                     RELREAGVEYAVVKNTMLRFAI"
BASE COUNT          706 a          918 c          970 g          515 t
ORIGIN      
        1 ggccgccgca tcttccacgc gcgtctttgc cgccggggca cggcctcgcg cgggcggtcc
       61 cgttcggcgg cgggaagagc ccggcccgcc cggtggggca ggcgggcgcg gcaggcggac
      121 ccgcttggcg gctgcggggc ggcgggcggc gcttttgccc tgccgctcgg ccccggggca
      181 aaaatatatc ccgcgcgcgg gaaaaagggc cgtcgggccc ttgacgcgcg ggggcgggat
      241 gtgctatagt taattcacct ctttgcaggt ctattttttt gtgcctattt tttggcgctt
      301 aaaacgggcc cgccacttta aaaaagaggg aggccagccc gccgtgaccg cgctttgcgg
      361 gggcggcaga ggcgaaacga accgtttaat ggaggtgccg aagaatgaga gtcaaaatta
      421 cccttgcatg cacggagtgc aaacagcgca actacaacac catgaagaac aagaagaaca
      481 gccccgaccg gctggagatg aacaagtact gccgcttctg caagaagcac actctccaca
      541 gagagacgaa gtgagaaagg cggaggacaa tggctgagaa agctgaaaaa gccgtcaaga
      601 cggaaaagcc cgccaagacg gagaagaaag agaagaagcc gttcttcctg gtgcgcttct
      661 tccgctcgat caaaaagttc ttccgcgata cgttcaccga gatgaagaag atcgtctggc
      721 ccagcaaaaa gcaggtgatc aacaacacgc tggtcgtcat cgccgtggtg atccttgccg
      781 ggctggtgat cttcggcctg gacaacctgt tcggcctgat cctgaagctg atcctgcgca
      841 cctgataagg gcgcggcgca atgaaagggg agacggagat ggcagaagaa ggtctgtggt
      901 atgtggtgca tacctattcc ggctacgaaa acaaggtcgc tcagaacctg gagactttgg
      961 tggaaaaccg ccgcctccat gatctgatcc aggaggtcaa ggtgcccacc gagatcgtcc
     1021 ccgagatcaa ggacggcgtc aagcgcgagg tggagcgcaa gcttttcccc ggctatgtgc
     1081 tggtgaagat ggtcatgacc gacgattcct ggtacgtggt gcgcaacacc cgcggcgtca
     1141 ccggctttgt ggggccctcc tcaaagccca tcccgctgac ccaggcggag gtcgacgcgc
     1201 tgggcgtgga gacccacgag gcgcaggtgg attacaaggt gggggacaac gtcgaggtca
     1261 ccgccggccc gctggagggc ttcatcggcc tggtggagga gatcgacatg gaaaaacaaa
     1321 aggtcaaggt gaaggtctcc atgttcgggc gcgagacgtc cgcggagctg gacctgtcgc
     1381 aggtgaaacc cctgtaaatc cgcaattttt tcgcagaaaa ccgcttttgc ggtttttgat
     1441 aggagccccc tttcccgggg ctccgtggga ggatgggggc atcctcatcc gcaacgaacc
     1501 acaatttttt ggaggtgcag acaatggctc agaaggtagt ggggtatatt aaactccaga
     1561 ttcccgccgg caaggcaacc ccggcaccgc ccgttggtcc cgctctcggc cagcacggcg
     1621 tcaacatcat gtctttcacg aaagaattca acgagcgcac ccaaaaggat atgggctaca
     1681 tcatccccgt ggtgatcacc gtctatgccg accgctcgtt cagcttcatc accaagactc
     1741 ctccggcccc ggtcctcatc aaaaaggcct gcggcatcga gtccggctcc ggcgcgccca
     1801 acaagaccaa ggtggccacc atcaccaagg atcagctgcg cgagatcgcc cagaccaaga
     1861 tgcccgacct gaacgcctcg tccgtcgaga gcgccatgtc catgatcgcc ggcacctgcc
     1921 gcagcatggg cgtcacggtc gccgaataag ctttgcggac ccgccagaag tgggaggaaa
     1981 tttccgattt aaccacagga ggatagaata tgaaacacgg taagaagtac gtcgactcgg
     2041 cgaagctggt cgaccgcacc aaaacatatg aggccgaaga ggccctggcg ctgtgctgcc
     2101 agaccgcaaa ggccaaattt gacgagaccg tcgagctgca cgtgcgcctg ggcgtcgaca
     2161 gccgccacgc cgaccagcag gtccgcggcg ccgtcgtgct gccccacggc accggcaagg
     2221 acgtgcgggt catcgtcatc gccaagggcg acgcggccaa ggccgccgag gatgccggcg
     2281 cgatgatggt gggcgacgag gagctcgccg ccaagatcca gaacgagggc tttatcgact
     2341 ttgacgtcct catcaccacc ccgaacatga tgggcgtcgt gggccgtctg ggcaagatcc
     2401 tcggaccccg cggcctgatg ccgaacccca aggccggcac cgtcaccatg gacgtgggcc
     2461 gcgccgtgac cgaggccaag gccggtaaga tcgagtaccg cctggacaag gccaacatca
     2521 tccacgtgcc catcggcaag gcctcctttg gcgccgagaa gctgggcgac aacctgaaga
     2581 ccgtcatgga cgccatcgtg aaagcgaagc ccgccgccgc gaaaggccag tatatcaaga
     2641 gcgccaccgt ggcctccacc atgggccccg gcgtcaagct gaacagcgcc cgttacggcg
     2701 cctgagcggc aggctcttga caaacccctc aagtgggggt aaaataaaaa agctgtccca
     2761 agacagcagg tatcgccaag atataaaggg gttttgcccc gcctgccgag gatgagcgcc
     2821 caaaggttcg cactgcgacg ttttgtgcct ctccccggcc gccggggaga ggctttttta
     2881 tgtgaaaata gggaaacgag gtgaaaaatt ttgccaagtg agaagattct gtcccagaaa
     2941 caggcttacg tcaaggagct ggaggagaag ctgaaaaact ctgtggccgg cgtcgtcgtc
     3001 gactacaagg gcatcagcgt tgcggacgac accaagctgc gccgcgagct gcgcgaggcg
     3061 ggcgtcgagt acgccgtcgt caagaacacc atgctgcgct ttgcgatcg
//
LOCUS       DBMDYA010000320         3104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7027_length_3104_cov_1.332334, whole genome shotgun sequence.
ACCESSION   DBMDYA010000320 DBMDYA010000000
VERSION     DBMDYA010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7027_length_3104_cov_1.332334"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="ACZPFK_08355"
     CDS             complement(<1..342)
                     /locus_tag="ACZPFK_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR1406362.1"
                     /translation="MAISPDYLQELSQRTDIAELVGGYVQLRRRGRTYTGLCPFHSEK
                     TPSFVVYPETASFYCFGCGAGGDAITFVKRINNLDYIEAVKFLAARAGMPLPDDRDDT
                     GKIKRRILAMNK"
     gene            complement(399..1403)
                     /locus_tag="ACZPFK_08360"
     CDS             complement(399..1403)
                     /locus_tag="ACZPFK_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1406363.1"
                     /translation="MTVRERTEEIERQTLSPYACLSQDSRGRERPEEPCPLRPCFQRD
                     RDRIIHCKSFRRLKQKTQVFLAPEGDHYRTRLTHTLEVAQIARTIARSLRLNEDLTEA
                     IALGHDLGHTPFGHAGERALNGLCPQGFAHYLQSVRVVQKLEKYPEGLNLSKEVLNGI
                     ACHTRGPEAFTLEGQVVKYADHIAYINHDIEDAVTAGVLHQQDLPREAVRVLGATKSR
                     RITTLITSLVENSAGGALRMAPEVYEQYEALHSFMYATVYVDKRAKKEEKKVDKLIGE
                     LYGYFKAHPNRMPGQFQQIFAEEGADRAVTDYISGMSDEFAIRTFEELFVPRGWSLQY
                     "
     gene            complement(1607..2230)
                     /locus_tag="ACZPFK_08365"
     CDS             complement(1607..2230)
                     /locus_tag="ACZPFK_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HIR1406364.1"
                     /translation="MLESYTTIEGTAEAAIVEKKSEFIAAASFADTKQAALRFLEEVR
                     ARHRTANHNVYAYLLRQGGRTRYSDDGEPQKTAGLPVLEVLRHSGLTDLIVVVTRYFG
                     GTLLGTGGLVRAYTTAAQAALERAHRVTVRPCVDMSAVLPYALYEQGARLAAAAGAKV
                     RDTRFTDEVTLELTLLQGAEAPLLEKLSELCRGRLRAQVGEPYYAPF"
     gene            complement(2683..2886)
                     /gene="rpmE"
                     /locus_tag="ACZPFK_08370"
     CDS             complement(2683..2886)
                     /gene="rpmE"
                     /locus_tag="ACZPFK_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1406365.1"
                     /translation="MKEGIHPKYEDAVITCACGEVIHTRSTKKELRVDVCSKCHPFYT
                     GKQKLVDSGGRVDRFKKRFGIED"
BASE COUNT          539 a         1029 c          897 g          639 t
ORIGIN      
        1 cttattcatt gccaggatgc ggcgttttat tttgcccgtg tcgtcccggt cgtcgggcag
       61 cggcatcccg gcccgggcgg ccaaaaactt caccgcctct atgtagtcca ggttgttgat
      121 ccgcttgaca aaagtgatgg cgtccccgcc cgccccgcag ccgaagcagt aaaagctggc
      181 cgtctcgggg tagaccacaa aggaaggggt cttttcactg tggaacgggc agagcccggt
      241 ataggtgcgc ccccggcggc ggagctggac atagccgccc accagctccg cgatatcagt
      301 gcgctgcgag agctcctgca gataatccgg tgaaattgcc atgtgccagc ctcctctcct
      361 gcccgcaggc ccggcggccc ggcaggcgtc tcttcctctc aatattgcag cgaccagccc
      421 cttggcacga acagctcctc aaatgtccgg atggcaaact cgtcgctcat gccggagata
      481 tagtcggtga cggcccggtc cgctccctct tcggcaaaaa tctgctggaa ctggcccggc
      541 atccggttgg ggtgcgcctt gaaatagccg tagagctccc cgatcagctt gtcgactttt
      601 ttctcctctt ttttcgcccg cttgtccaca tacaccgtgg cgtacataaa gctgtgcagc
      661 gcctcatact gctcgtagac ctccggcgcc atacgcagcg cccccccggc gctgttctcc
      721 accagcgagg tgatcagggt ggtgatgcgg cggctcttgg tcgcgcccag cacccgcacg
      781 gcctcgcgcg gcagatcctg ctgatgcagc accccggctg tgaccgcgtc ctcgatgtcg
      841 tggttgatat aggcgatgtg gtcggcgtat ttgaccacct ggccctccag cgtgaacgcc
      901 tcggggccgc gggtgtggca ggcgatgccg ttgagcacct ctttggagag attgagcccc
      961 tcgggatatt tttccagctt ctgcaccacc cgcacgctct gcagatagtg ggcgaagccc
     1021 tgggggcaca ggccgttcag cgcccgctcc cccgcgtgcc caaagggggt gtggcccagg
     1081 tcatgcccca gggcgatggc ctcggtcagg tcctcgttga ggcgcaggct gcgggcgatg
     1141 gtgcgggcga tctgcgccac ctccagcgta tgggtcagcc gggtgcggta atggtctccc
     1201 tccggcgcga gaaagacctg ggtcttttgc ttgagccgcc gaaagctttt gcagtggatg
     1261 atgcggtccc tgtcccgctg gaagcagggc cgcagcgggc agggctcctc cggccgctct
     1321 ctgccgcggc tgtcctgaga caggcaggca tagggcgaga gggtctgccg ctcgatctct
     1381 tcggtgcgtt cccgtacagt cacaaatggt cacctccgcc gcgcccgtgc cccatcggcg
     1441 cttccaggcg aacaaaaaac agcttccata tatactatac gacgtttttg cgcaaaaccc
     1501 ttttctttca cagaaatttc cagaaaaatt ttcttcgcgt caaaaaaagc tcagggtttt
     1561 ttaccgccgc ttttgcccac cggtctccgg gcgcggggcg ggccgctcaa aagggggcgt
     1621 aatagggctc gcccacctgg gcgcgcagcc tgccccggca gagctcggag agcttctcca
     1681 gcagcggcgc ctcggccccc tgcagcagtg tcagctccag tgtcacctcg tcggtgaaac
     1741 gggtgtcccg caccttggcg cccgctgcgg cggcaagccg cgccccctgc tcgtaaaggg
     1801 cgtagggcag caccgccgac atatccacgc aggggcgcac cgtcacccgg tgcgcccgct
     1861 ccagcgccgc ctgcgccgca gtggtatagg cccgcaccag gccgccggtg cccagcaggg
     1921 tgccgccgaa atagcgggtg accaccacga tcaggtcggt gaggccggaa tggcgcagca
     1981 cctccagcac cggcaggccc gccgtcttct gcggctcgcc gtcatcggaa tagcgggtgc
     2041 ggccgccctg ccgcagcaga taagcataaa cgttgtggtt ggccgtgcgg tgccgcgcgc
     2101 gcacctcctc caaaaagcgc aatgccgcct gcttggtgtc tgcaaatgag gctgcggcaa
     2161 tgaattcgct ctttttttct acaatggcgg cctccgccgt cccttcaatc gtggtgtagc
     2221 tctccaacac ccgcgctccc ctcctgtttt ttgccgccga cgccgccccg aagcgaccgg
     2281 gaagcttgcg ccccgtcttc tcgaatcggc gcacggccgg attttttaaa agaaagccgc
     2341 agccaaaatt ttcgccggat gcggcattcc cttttcaccg gcaagcaggc caggcgaccg
     2401 ttttgggcct cttgtcctcc caaagcccgc cgctggccct ttcgatggaa attcctgcgg
     2461 cgggtataaa gccgaaagtg cgcttttcgc accgtggcaa acatgtccca cagcaccctc
     2521 cccgggcggt ctttttcaaa cccgtcctag ccgcacccta tggttctttc cacggctgcg
     2581 ataacgcggg tgcagggcgg gtcgcaatgg ggtctcttac aaaaacaaaa aggcctgaga
     2641 gagcgttgcc ctctcaggtc tctttttctg ttcagttttg ccttagtcct caatgccgaa
     2701 acgctttttg aagcggtcga cgcgtccgcc ggaatccacc agcttctgtt tgccggtgta
     2761 aaagggatgg cacttggagc acacatccac gcggagctct ttctttgtgg aacgggtatg
     2821 aatcacctcg ccgcaggcgc aggtgataac ggcgtcctca tatttgggat ggataccttc
     2881 cttcatcttc gttcacctca ctctggttct gattattagc gcatgtgatt tggcatacgc
     2941 agcatcacaa cctgatctca ccggggtcgg gacaccggaa tacacgattg ctatagtagt
     3001 ctaccacaac gcgcacaaaa atgcaactgt ttttccgcca ccccgatacg ccccaaaccg
     3061 cccgccgctg ccccggcaca atccggtgcc tgtcgtttcc ccgc
//
LOCUS       DBMDYA010000321         3103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7029_length_3103_cov_1.590939, whole genome shotgun sequence.
ACCESSION   DBMDYA010000321 DBMDYA010000000
VERSION     DBMDYA010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7029_length_3103_cov_1.590939"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            82..339
                     /locus_tag="ACZPFK_08375"
     CDS             82..339
                     /locus_tag="ACZPFK_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406366.1"
                     /translation="MNLRKQKRKLTRVFAHEVFHGRSRSGWTQQAVADRVGVSLRWYQ
                     KIEKGEVLPSFFVGIGLMWIFGITPEALVREVFGDVPVPTR"
     gene            317..550
                     /locus_tag="ACZPFK_08380"
     CDS             317..550
                     /locus_tag="ACZPFK_08380"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1406367.1"
                     /translation="MYQYLPVEERLYSPYLGRYRSFGIAAFKVCGEQREQVAFTSDVS
                     TDEAFAAQLAQRCTVAQLNPVHLKDVVLDSIGR"
     gene            complement(600..908)
                     /locus_tag="ACZPFK_08385"
     CDS             complement(600..908)
                     /locus_tag="ACZPFK_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406368.1"
                     /translation="MDMILLTLMMAVTVEGLVEYGKSFGKAVLDKAWKTTGTQAAAMA
                     LGVGLCFAVGADFYAALGVTFAAPWLGCLLTGVFASRGANYVSDLIKKLQSFGDVATK
                     "
     gene            complement(922..1626)
                     /locus_tag="ACZPFK_08390"
     CDS             complement(922..1626)
                     /locus_tag="ACZPFK_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1406369.1"
                     /translation="MENAMFPMEYLRVTQRAGVGSHLGSKALDFGGRDGSCDRVLAPF
                     SGTVVRTRKNANGEMYLQSDQPVRWADGTMDYLTVTFMHDSSFNVPEGAHVIQGQYIY
                     DEGGMGGGNPCKFANHLHMEVSRGRVAARQIRNGYGVYCTSNQVDIDSALMLGDDVTI
                     LAGGGYNWRRRSDATASLGTLSADNTYKARSGDSWWRIAAQQLGSGLKCYALAKANGK
                     TIFSVIHPGDVLTLSI"
     gene            complement(1686..2777)
                     /locus_tag="ACZPFK_08395"
     CDS             complement(1686..2777)
                     /locus_tag="ACZPFK_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1406370.1"
                     /translation="MRQNRRVKTVKVIIGYKADNTPIRRPFYGRTKEAAQQRAEKWLE
                     IHGTPSHNADILTVGGWAARWLRVYKRPDVSAATYQTTYEITVRRHILPHLGDRVMMD
                     LTPVDVKAFYGKVSHLSQSVCDKIKICLMGILETAIDNGLCQTNPARKIKIRSTAAPR
                     IKEVYTEAQIETAKAWFVQAMPEVVLALETGVRRGELCGWLKSDFAPRRHLYQVARAV
                     RRGASGPELGAPKAHSNRTNPLSPHARQAYELLCARHPDSPYLLPGPDGGLMRPDTWS
                     RRLKAEMERLAAAHPDMPQLTAHELRHTYGTSLRRHGADIYSIAKILGHRDVEVTARI
                     YVHNELDELKRATRYTRRQQPLAVQTEAA"
BASE COUNT          598 a          927 c          883 g          695 t
ORIGIN      
        1 actaacatga ataacgtgtt cgtatcacaa tagttgaatt tggtatattc ttccagtata
       61 aaacaaagga ggaatacacc catgaatttg cggaaacaaa aacggaagct cacgcgggtt
      121 tttgcccacg aagtcttcca tggacgatca cggtcgggat ggacacaaca ggcggtggct
      181 gaccgtgtcg gcgtctcgct gcgctggtat caaaagatag agaaaggcga ggtgctgccc
      241 agtttttttg tggggatagg cctgatgtgg atttttggta tcacgccgga agctttggtg
      301 agggaggtgt ttggagatgt accagtacct acccgttgaa gaacggctgt attccccgta
      361 tctaggccgc tatcgctctt tcggcattgc ggcctttaag gtctgcgggg agcagcggga
      421 gcaggtagcg tttacttcgg atgtgtccac ggatgaggcg ttcgcggcgc agttggccca
      481 gcgctgcacc gtcgcccagc tcaacccagt ccatctgaaa gatgtagtgc tggattctat
      541 cggaagataa aaaataggcc ccaagcccgc aggaaacccc tgcaagcctg gggccatacc
      601 tattttgtgg cgacgtcgcc gaagctctgg agttttttga tcaggtcgct gacatagttt
      661 gcgccgcggc tggcaaatac accggtaagc aggcagccca gccagggggc ggcaaaggtc
      721 actcctagtg ctgcgtaaaa atccgcacct acagcaaagc agagccccac ccccagtgcc
      781 atcgccgccg cttgggtgcc agtggttttc cacgccttgt ccagcaccgc tttgccaaag
      841 ctcttgccgt actccaccag gccctccacc gtcaccgcca tcatcagcgt cagtaagatc
      901 atgtccatat gtttcctccc attaaattga aagcgtaagc acgtccccag gatgaatcac
      961 gctgaaaatc gttttaccgt tggctttggc cagggcatag cattttagac cgctgcccaa
     1021 ctgctgggcg gcgatacgcc accaactgtc tccactcctg gctttgtagg tattgtcggc
     1081 ggaaagcgta cccaaagagg cagtagcgtc actgcggcga cgccagttat aaccaccgcc
     1141 tgcgagaatt gtaacgtcgt ctcccaacat caaagcgctg tcgatatcca cctggttgct
     1201 agtgcaatag acgccgtatc cattacggat ttgtcgggca gccacgcggc cccgacttac
     1261 ctccatatgt aggtggttgg caaatttgca gggattgccc cctcccatgc cgccctcgtc
     1321 atagatatat tgcccctgga taacatgcgc gccctcgggg acgttaaaag agctatcatg
     1381 catgaatgta actgtcagat aatccatggt tccgtcggcc catcgtaccg gctgatcgga
     1441 ctgaagatac atctctccgt tagcattttt ccgggtgcgc actaccgtac cggaaaaagg
     1501 tgcaagaacc ctatcacagc tgccatctcg cccgccaaag tcaagggctt tgctgcccag
     1561 atgtgatcct actcctgctc tttgggttac tcgcaaatac tccattggaa acattgcatt
     1621 ttccattttt tgacctcctg atgtttgtta tattttagcg gccgcggggc ggtgggcggc
     1681 ggatcctacg cggcctcggt ctggacggcc agcggctgct ggcggcgggt gtagcgggtc
     1741 gcccgcttga gctcgtccag ctcgttgtgc acgtagatgc gcgccgtcac ctccacgtcc
     1801 cggtgaccga gtatctttgc aatgctgtag atgtccgccc cgtgccgccg caggctggtg
     1861 ccgtaggtgt ggcgcagctc gtgggccgtc agctggggca tgtccgggtg cgcggccgcc
     1921 agccgctcca tctccgcctt gagccggcgc gaccaggtgt cgggccgcat caggccgccg
     1981 tccggccccg gcagcaggta ggggctgtcc gggtggcggg cgcacagcag ctcgtaggcc
     2041 tgccgcgcgt gcgggctgag cgggttggtg cggttgctgt gcgccttggg cgcgcccagc
     2101 tccggccccg aagccccgcg ccgcaccgcc cgcgccacct ggtacagatg ccgccgcggc
     2161 gcgaagtccg acttgagcca gccgcacagc tcaccccggc gcaccccggt ttccagcgcc
     2221 agcaccacct ccggcatggc ctgcacaaac cacgctttgg ccgtctcgat ctgcgcctcg
     2281 gtgtagacct ccttgatacg cggggccgcc gtgctgcgta tcttgatttt gcgcgccggg
     2341 tttgtctggc acagcccgtt gtcgatggcc gtttccaata tgcccatcag gcagattttg
     2401 atcttgtcgc agacgctctg cgagaggtgg gacaccttgc cgtaaaacgc cttgacgtcc
     2461 acgggcgtca ggtccatcat cacccggtca cccagatgcg gcaggatgtg tcgccgcacc
     2521 gtgatctcgt aggtagtctg gtaggtcgcc gccgacacgt ccggccgctt gtacacccgc
     2581 agccaccggg ccgcccaacc gcccacggta aggatgtctg cgttgtgcga tggtgtgccg
     2641 tggatctcca gccatttttc tgcccgctgc tgtgccgcct ctttcgtgcg gccataaaag
     2701 ggacgcctga tgggggtatt gtccgccttg taaccgatga tgaccttgac cgtcttaact
     2761 cgcctgtttt gccgcatgat aggccctcca ctactgtttt tagggttatc ataccacact
     2821 gcctgtgagc ctgtttcctc ctctaatttg ggtagcgttg gaggcgcaac aacacatacc
     2881 cttacaagag cagagctgcc gacgggcatc agcggcaaaa ttatgatgca ttggtctgag
     2941 atgggcacac ctatacaggg agtggagggc gtattttctg ccccccaaaa attgccgtct
     3001 tatcgctcgg ggggagattt gacggagggc atacaatcta tcgggcagat tggtttttct
     3061 ttaggcggca agagcacccc tcacaataac atgccgccct aca
//
LOCUS       DBMDYA010000322         3102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7032_length_3102_cov_1.408864, whole genome shotgun sequence.
ACCESSION   DBMDYA010000322 DBMDYA010000000
VERSION     DBMDYA010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7032_length_3102_cov_1.408864"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="ACZPFK_08400"
     CDS             complement(<1..1307)
                     /locus_tag="ACZPFK_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1406371.1"
                     /translation="MTTLIKNGVVITADPQDRVFLKGWVLLRDDRIEALGEGCPDAAL
                     RADRVVDAAGMALLPGIVDVHTHVAGTLFKGLTEDPENGFYGLALPMEQHFTEEYAYP
                     LARLGAVECLKAGITCINDVYHYARQTARAVDELGMRAVLAQQIHEVDLASIRTGAYT
                     PDPREGEARLEEACRLIEEYHGRDGRIFCRFGPHAVDTVSMPLARRIADLGHRYHVGF
                     HTHAGQSAAELAHLKETCGLSPIEYLCETGLMGKDLVVAHCILCTDHDLELLQRGGAT
                     LAHCPEIYCKRGFFPPVEKIYGSGIHLAYGTDWVTMDPWTNMRTAVMAARLRGCKLCD
                     PSAHTALRLSTIEPAKALGLDDRIGSLEVGKQADLVLLDLRSPSLTHLFDDPVATIVY
                     NANRNDVDTVRVAGRTLVENRRLRTADEAEVLAEGQRAAGAIY"
     gene            complement(1373..2452)
                     /locus_tag="ACZPFK_08405"
     CDS             complement(1373..2452)
                     /locus_tag="ACZPFK_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406372.1"
                     /translation="MKLKRLAASAAALLFLAGLLSGCGEKQFDSVLTVDGEAISPAVY
                     RMYQMQAYVQASQRVSSLSDTIGGVAAADWIYDTTVSLLRARRYYELEFEAQGLSFTE
                     AERAQLETQTGEAWASAHAIYEKNGVDEAAYRQYQLTNAKGRKLFEAYCADLTDEQVK
                     AYLEGHFVLIEYTQLPRIDSDGAALDEEGLSAVDRIAERALDELAASGGDADFAEVCA
                     EAVADSFELGGFAVPDLSSQDNYISSSYLTLDGTGNAFTEALAQQVAAVEIGGFGSYA
                     DERVTVLFHRLPNYETEQAFQDLRPSVLATMRGEAFDTACSQVWNSYEVLPDEEAVAW
                     YDPKKLDLSAPSPTEAPSSAPLGEG"
BASE COUNT          546 a          951 c         1081 g          524 t
ORIGIN      
        1 tcatagatcg ccccggcggc ccgctggcct tccgccagga cctcggcctc gtcggccgtc
       61 cgcagacggc ggttctccac cagcgttctg ccggccaccc tcaccgtgtc cacatcgttg
      121 cggttggcgt tgtagacgat ggtggccacc ggatcatcga agaggtgggt caggctgggg
      181 ctgcgcaggt ccagcagcac caggtccgcc tgcttgccca cctccagcga gccgatgcgg
      241 tcgtccaggc ccagcgcttt ggcgggctca atggtggaca gccgcagcgc cgtgtgggcg
      301 ctggggtcac agagcttgca gccccgcagc cgcgcggcca tgacggcggt gcgcatgttg
      361 gtccaggggt ccatagtgac ccagtcggtg ccgtaggcga ggtggatgcc gctgccgtag
      421 atcttttcca ccggcgggaa aaagccgcgc ttgcagtaga tctcggggca gtgggccagc
      481 gtggcgccgc cccgctgcag aagctccagg tcgtggtccg tgcacaggat gcagtgggcg
      541 acgaccaggt ccttgcccat caggccggtc tcgcacagat attcgatggg cgagaggccg
      601 caggtctctt tgagatgggc gagctcggcg gcggactggc ccgcatgggt gtgaaagccc
      661 acatgataac ggtggcccag gtcggcgatg cggcgggcaa gaggcatgga gacggtgtcc
      721 acggcgtggg gaccgaagcg gcagaaaatg cgcccgtccc tgccgtgata ctcttcgatc
      781 aaacggcagg cctcctccag gcgcgcctcg ccctcccgcg ggtcgggtgt gtaggcgccg
      841 gtgcggatgc tggccagatc cacctcgtga atctgctggg ccagcacggc gcgcatgccc
      901 agctcatcca ccgcccgcgc cgtctggcgg gcataatggt agacgtcgtt gatacaggtg
      961 atgcccgctt tcaggcattc gacagccccc agccgcgcca gggggtaggc gtattcctcg
     1021 gtgaagtgct gctccatggg cagggccagc ccgtaaaagc cgttttccgg gtcctcggta
     1081 agccccttga acagcgtgcc tgcgacgtgg gtgtgcacgt ccacaatgcc cggcagaagg
     1141 gccatgccgg cggcgtccac cacccggtcg gcccgcagcg cggcgtcggg gcagccctcg
     1201 cccagggcct cgatgcggtc gtcccgcagc agcacccagc cttttaaaaa gacccggtcc
     1261 tgcgggtcgg cggtgatgac gacgccgttt ttgatgagag ttgtcatagc ggtgggctcc
     1321 tttcactgcg cacagaataa gacggggccg ccctcggggg cggcccaaaa catcacccct
     1381 cgcccagggg ggcggaggag ggggcttcgg tgggagaggg ggcgctcagg tccagctttt
     1441 tggggtcata ccaggccacc gcctcttcgt ccgggaggac ttcataggag ttccagacct
     1501 ggctgcaggc cgtgtcgaaa gcctcgccgc gcatggtggc gagcaccgag gggcgcaggt
     1561 cctgaaaggc ctgctcggtc tcgtagttgg gcaggcggtg aaagaggacc gtcacccgct
     1621 cgtcggcata gctgccaaag ccgccgatct ccacagccgc cacctgttgg gccagcgcct
     1681 cggtgaaagc gttgccggtg ccgtccagcg tgaggtaaga ggaggagatg tagttgtcct
     1741 gcgaggagag gtcgggcacc gcaaagccgc ccagctcaaa gctgtcggcc accgcctcgg
     1801 cgcacacctc ggcaaaatcg gcgtccccgc cggaggcggc cagctcgtcc agggcccgct
     1861 cggcgatgcg gtccacggcc gacaggccct cttcatccag cgccgcgccg tccgagtcga
     1921 tgcgcggcag ctgggtgtac tcgatgagga cgaaatggcc ctcgagatac gctttgacct
     1981 gctcgtccgt caggtcggcg cagtaggcct caaacagctt gcgccccttg gcgttggtga
     2041 gctggtattg ccggtatgcc gcctcatcca cgccgttttt ttcgtaaatg gcgtgggcgg
     2101 aggcccaggc ctcgccggtt tgggtctcca gctgggcgcg ctccgcctcg gtaaaggaga
     2161 gcccctgtgc ctcgaactcc aactcgtaat agcggcgcgc gcgcagcagc gagaccgtgg
     2221 tgtcgtagat ccagtcggcg gcggcgaccc cgccgatggt gtcgctgagg gaggaaacgc
     2281 gctgggaggc ctgcacatag gcctgcatct gatacatgcg gtagaccgcg ggggaaatgg
     2341 cttccccgtc caccgtgagc acactgtcga actgcttttc accgcagccg gacaaaagcc
     2401 ctgccaggaa caacagggcc gcggcggagg cggcgagcct tttcagtttc aagtgggatc
     2461 aactccatac attggtaaca aaacggttac acagcaattt cattatagag aaaatccaca
     2521 gatttgtaaa gagaaatacg cataagttac aacatcttct acagagggcc gctgttttga
     2581 cggcttttcc gcaaaaaaag agcggtcttg cggccgcgcc gagacgcctg ggcgggccgg
     2641 tgggccgggg gcgtctgccc gggctcgggg cgcaggcatg ggggcagaga ttgtcgcagc
     2701 gcagaacgcc cagatgcgga gacacaaggc atggtgtggg atgcagggtc taggtgcgag
     2761 gcgtcggggc cgggtcgagg cccagggccc ggcgtgagtg cagagggtac aggtacggga
     2821 gacaggatcc aggcgcagag cacagggccc aatgcggagg gcgggtcctg ccacgccgcc
     2881 gcagggtcgg cagaaggacg ctccgcagaa aggcttttgc tccctgatgc agcccctctg
     2941 gcccctgcgg ccaatggcgt tacagagagg aatacacgga gatttttgga gggaaaggga
     3001 ggcgccttgg caagagaaat gcggccgggc tgttttagga gctatgtgtt tcggctgccg
     3061 cgcggctacg gaggtcaggg gataaagaac agttcccgtc tt
//
LOCUS       DBMDYA010000323         3097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7042_length_3097_cov_1.771696, whole genome shotgun sequence.
ACCESSION   DBMDYA010000323 DBMDYA010000000
VERSION     DBMDYA010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7042_length_3097_cov_1.771696"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="yiaK"
                     /locus_tag="ACZPFK_08410"
     CDS             <1..965
                     /gene="yiaK"
                     /locus_tag="ACZPFK_08410"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014653.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HIR1406373.1"
                     /translation="LERAGFDGARAARLADVFARNSLEGVYSHGVNRFPRFLNSVASG
                     AVDRDAVPERAAALGGMEVWDGHFGPGPLIAEDAMARAVELARTHGIACVSVRNSNHW
                     MRPGRYGRQAAGAGMAAILFTNTSANMPAWGALDARLGNNPIVLAVPREKGPVVVDMA
                     MSQFAYGKLEVAALAGEQLPMEGGFDETGALTRDPQAILRTKRILPTGYWKGSAMSVA
                     LDLMAAALSMGRTVSAVSRDPLQERGLSQVFVAINFRGLLPAGEVDRLLDEALQDLLA
                     SAPAPGEPPVRYPGQNLEQTVRRNLENGVPVNEATWRKIEAFLQ"
     gene            complement(1052..1408)
                     /locus_tag="ACZPFK_08415"
     CDS             complement(1052..1408)
                     /locus_tag="ACZPFK_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1406374.1"
                     /translation="MKPHFSERERKVLDLRENREMTYTAIAKEIGLSVSRASQIYRQA
                     RLKQNMVERREAERQKALLNEYTGFSGEELDFFFEGLRWYQRQLRAELPEVAETYLAI
                     EALKKKLVAMMYRETL"
     gene            complement(1426..1764)
                     /locus_tag="ACZPFK_08420"
     CDS             complement(1426..1764)
                     /locus_tag="ACZPFK_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406375.1"
                     /translation="MDAILIGKQIRLARRQKGMSVEQLAEQIGIEPASLSHIESGNRQ
                     TKLSTLVKIADKLEVSLDYLVARETPETSRLILKNFSDELAALTPKQAYLLLDMAAPV
                     IKYLKENTTE"
     gene            2433..>3097
                     /locus_tag="ACZPFK_08425"
     CDS             2433..>3097
                     /locus_tag="ACZPFK_08425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR1406376.1"
                     /translation="MQGEKRPDPDRLLHQVQREEAPPAGRGRLKIFFGYAAGVGKTYA
                     MLKAAHAARQAGVEVVAGYIEPHTRPATLALLDGLEQLPPLVPTHKGVALREFDLDAA
                     LARRPQLLLVDELAHTNAAGCRHRKRYQDVEELLRAGIDVYTTVNVQHLESLNDIVAS
                     ITGVTVRERVPDSVFDSADQVELVDLEPQELIARLQAGQIYAAGQARRALAHFFTHEN
                     LVAL"
BASE COUNT          524 a          961 c         1040 g          572 t
ORIGIN      
        1 cgctggagcg ggcgggcttt gacggggcgc gggccgcgcg gctggcggat gtgttcgccc
       61 gcaactcgct ggagggcgtc tattcccacg gggtgaaccg ctttccccgc tttttgaaca
      121 gcgtggcgag cggcgcggtt gaccgggacg ccgtgccgga gcgggccgcg gcgctggggg
      181 gcatggaggt ctgggacggg catttcgggc ccggcccgct gatcgccgag gacgccatgg
      241 cccgcgcggt ggagctggcg cgcacccacg gcatcgcctg cgtctcggtg cgcaacagca
      301 accactggat gcgcccgggc cggtacggca ggcaggcggc cggggcgggg atggcggcca
      361 tcctgttcac caatacctcg gccaatatgc cggcctgggg cgccctggac gcacggctgg
      421 gcaacaaccc catcgtgctg gccgtcccac gtgaaaaggg gccggtggtg gtggatatgg
      481 ccatgagcca attcgcctac ggcaagctgg aggtggccgc cctggccggg gagcagctgc
      541 ccatggaggg cggctttgac gagacgggcg cgctgacgcg ggacccgcag gccatcctgc
      601 gcacaaagcg catcctgccc accggctact ggaaaggctc ggccatgtcg gtcgcgctgg
      661 atctgatggc ggccgcgctg tcgatggggc gcaccgtcag cgcggtcagc cgtgacccgc
      721 tgcaggaacg ggggctgtcc caggtgttcg ttgcgatcaa cttccgcggc ctgctgcccg
      781 ccggtgaggt ggatcggctg ctggacgagg cgctgcagga cctgttggcc tccgcgccgg
      841 cgccgggcga gccgccggtg cgctatcccg gccagaacct ggagcagacg gtgcgccgca
      901 atctggaaaa cggggtgccc gtgaatgagg ccacctggcg gaagatcgag gcttttttgc
      961 agtaaaaaaa gaaaagcgcc cccttggggg cgctctcatg gagcggatcg ggcggcgggg
     1021 cgaaagcccg gccgcttttt ccgtccgttt gtcaaagggt ttcgcggtac atcatcgcca
     1081 ccagcttctt tttcagcgct tcgatggcaa gatacgtctc ggccacctcg ggcagctctg
     1141 cgcgcagctg gcgctggtac caccgcagcc cctcaaaaaa gaagtccagc tcctcgccgc
     1201 tgaagccggt gtactcgttc agcagcgctt tctgccgctc ggcctcccgg cgctccacca
     1261 tgttctgctt cagccgcgcc tgccggtaga tctgcgaggc gcgtgaaacg ctgaggccga
     1321 tctctttcgc gatggcggta taggtcatct cccggttttc gcgcaggtcc aaaactttgc
     1381 gctcccgttc gctgaaatga ggcttcatga aatttatttc acctgctatt ccgttgtgtt
     1441 ttccttgaga tacttgatga ccggggcggc catatccaaa agcagatagg cctgctttgg
     1501 cgtgagggcc gccagctcgt cagaaaaatt tttcaggatc agcctgctcg tctccggcgt
     1561 ctcgcgggcg accagataat ccagcgaaac ctccagtttg tccgcgatct tcaccagggt
     1621 ggacagcttt gtctgtcggt ttccactctc tatgtgggat aaagaggcgg gttcgatccc
     1681 tatttgctcc gccagctgtt ccacgctcat ccctttttgc cgccgggcca gccggatttg
     1741 ttttccaatt aaaatagcat ccattttgca cgcacttatt cagttgatat ctcaagtata
     1801 gaaaatcccc atgcaatcga aaatgagcaa atagatattt tgtatccgct tgatgctttt
     1861 gcgcgaaatt agtatatttt caacataatt tggcggctct ttttggagga gcgggatgag
     1921 gaaaacttac ctgtgggatc agtatagagg atatgggagc ttttattaag gggatatggc
     1981 ccccttacag agaaaggaaa aggccggtga ggaccgcttc tgtcatccct tttttcctcg
     2041 ccgtctcgtt ccggtcccgg ctatgcctcc ccggcagggc gcgccggagg ggcaaaaagc
     2101 gcaaggcgcg ggcccggggt gaggaggcga ggggccgccg gagagcgcgg gccggcgcgc
     2161 aaaaaacggg cgcggtgcgg accgggcctt tgcggcgcgc cgtccgctcg ccgcctgccg
     2221 cactccgccc gttttcatgg ggcgcggcac ggccgcgctc ctgcgcccgg ccgcggcgtc
     2281 ttaacgccgc cttaatattt ccgccgcaaa ttttacgcgc cgctaacccc tcttgtggta
     2341 tagtaagata gaaaccaaaa tgcccgcggg gcggcgcgtt tgccgcaggc gccgaaaaag
     2401 cccctgtggg cgcaggaaag ggaggtgcgg ccatgcaggg ggaaaaacgg cccgacccgg
     2461 accggctgct gcatcaggtg cagcgggagg aagcgccccc cgccgggcgc ggcaggctga
     2521 agatcttttt cggctacgcg gccggggtgg gcaagaccta cgccatgctg aaagccgccc
     2581 atgccgcccg gcaggccggg gtggaggtgg tggccggcta tatcgagccc cacacccgcc
     2641 cggccacgct ggccctgctg gacgggctgg aacagctgcc gcctctcgta ccgacccaca
     2701 agggggtggc gctgcgcgag ttcgacctgg acgcggcgct ggcgcggcgg ccgcagctgc
     2761 tgctggtgga cgagctggcc cacaccaacg ccgcgggctg ccggcaccgc aagcgctatc
     2821 aggacgtgga agagctgctg cgcgcgggca tcgacgtgta caccaccgtc aacgtgcagc
     2881 atctggagag cctcaacgac atcgtggcct ccatcaccgg cgtcacggtg cgcgagcggg
     2941 tgccggacag cgtttttgac agcgccgacc aggtggagct ggtggacctt gagccgcagg
     3001 agttgatcgc tcgcctgcag gcggggcaaa tctacgccgc cgggcaggcg cggcgggcgc
     3061 tggcccactt ttttacccac gagaatctgg tggccct
//
LOCUS       DBMDYA010000324         3092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7057_length_3092_cov_1.903377, whole genome shotgun sequence.
ACCESSION   DBMDYA010000324 DBMDYA010000000
VERSION     DBMDYA010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7057_length_3092_cov_1.903377"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            48..1229
                     /gene="recA"
                     /locus_tag="ACZPFK_08430"
     CDS             48..1229
                     /gene="recA"
                     /locus_tag="ACZPFK_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1406377.1"
                     /translation="MAAEKAADKSAQDSKSKALETALAQIEKQFGKGAVMKLGQNVTM
                     QVDAISTGSLSLDLALGIGGLPRGRVVEIYGPESSGKTTVALHVIAEAQKAGGEAAFI
                     DVEHALDPVYAKALGVDIDSLLVSQPDTGEQALEICEALVRSGAIDVIVIDSVAAMVP
                     KAEIEGEMGDSHVGLQARLMSQALRKLTGVIGKTNTIAIFINQLREKVGIVYGNPEVT
                     SGGRALKYYSSVRIDVRRTEALKVNGEFIGNHTRARVVKNKVAPPFREGEFDIMFGEG
                     ISKEGEILDLAVKLDIIKKGGSWFSYGDTRLGQGRDNVKEYLKNNPDFAAEIERQVRE
                     NADKLLPGAARRAAAKPKAVEEAPVAPAPAAADAAPAAAAEKASKPAGRAANIDIAVE
                     D"
     gene            1229..1858
                     /locus_tag="ACZPFK_08435"
     CDS             1229..1858
                     /locus_tag="ACZPFK_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014668.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HIR1406378.1"
                     /translation="MAFTHRITAVERTRRGRFSIFADGEFLFSVDAETLAKSGLAEGD
                     EVSEAEIADLRENSDTRRAKDKALTFLSLRAYGSAELYDKLCRSFDEHTAAAAVAEMA
                     RLGLLDDAAFARRRAVYLHGQRKSLWEIRQKLLALGLDRELVEDALAALPQDEGGAIR
                     GVIEKQYLSKLARGETEKVVAALARRGFSAREARAAVAEFLSDPETAGD"
     gene            1926..>3092
                     /gene="rimO"
                     /locus_tag="ACZPFK_08440"
     CDS             1926..>3092
                     /gene="rimO"
                     /locus_tag="ACZPFK_08440"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1406379.1"
                     /translation="MATIAFISLGCPKNQVDADRMCHALLAAGHLTTADPAEADVIVV
                     NTCGFIESAKQEAIDTILEAVELKKGDHPPKVVVTGCLAERYREEIQKEIPEVDATVG
                     IGCNGDIAGLVDRVLQGEKLQAYGPKAGLPLEGKRVVSTPRHYAYLKIAEGCNNRCSY
                     CAIPLIRGPLRSRSLDSVVEEARWLASEGVKELIVVAQDITAYGDDLGQNLAAPLLDA
                     LQQVEGIRWIRLLYAYPERVTDAFIAAMARNDKVLPYLDLPIQHINSDILRSMNRKGD
                     RETVLSAIRRLRAGVPGLTLRTTLIAGYPGETQAQFDELCAFVKETGFDRLGCFAYSE
                     EEGTPAARMEQLPQELREQRAERIMEIQAAVMAEKQAARHGALLEVICDGLDEET"
BASE COUNT          574 a         1023 c         1052 g          443 t
ORIGIN      
        1 ttgctcttgc tgtccggcgc gttcaaaaaa gacatggagg taccactgtg gcagcagaga
       61 aagcagcgga taaatccgca caggacagca agagcaaggc ccttgagacg gcgcttgcgc
      121 agatcgaaaa gcagttcggc aagggcgcgg tcatgaagct gggccagaac gtcaccatgc
      181 aggtggacgc catctccacc ggcagcctct cgctggacct ggcgctgggc atcggcggcc
      241 tgccccgcgg ccgcgtggtg gaaatttatg ggcctgagtc cagcggcaag accaccgtgg
      301 ccctgcacgt catcgccgag gcccaaaagg ccgggggcga ggcggctttt atcgatgtgg
      361 agcacgcgct ggaccccgtc tacgccaagg ccctgggcgt ggacatcgac agcctgctgg
      421 tgagccagcc ggacaccggt gagcaggcgc tggagatctg tgaggcgctg gtgcgctcgg
      481 gcgccatcga cgtcatcgtc atcgactcgg tggcggccat ggtgcccaag gccgagattg
      541 agggcgagat gggcgacagc cacgtgggcc tgcaggcccg cctgatgagc caggcgctgc
      601 gcaagctcac cggcgtgatc ggcaagacca acaccatcgc catcttcatc aaccagctgc
      661 gcgaaaaggt gggtatcgtc tacggcaacc cggaggtcac ctccggcggc cgggcgctga
      721 agtactactc ctcggtgcgc atcgacgtgc gccgcaccga ggcgctgaag gtgaacggcg
      781 agttcatcgg caaccacacc cgcgcccggg tggtcaaaaa caaggtggcg cccccgttcc
      841 gcgagggcga gttcgacatc atgttcggcg agggcatctc caaagagggt gagatcctcg
      901 atctggcggt caagctggac atcatcaaaa agggcggctc ctggttcagc tacggcgaca
      961 cccgcctcgg ccagggccgt gacaatgtaa aagaatacct gaaaaacaac cccgactttg
     1021 cggccgagat cgagcgccag gtgcgcgaaa acgccgacaa gctgctgccg ggcgcagccc
     1081 gccgcgccgc cgccaagccc aaggcggtgg aggaagcccc cgtcgcgccc gcgcccgcag
     1141 cggcggatgc ggcccccgca gcggccgccg aaaaggcgtc caagcccgcg ggccgcgctg
     1201 ccaacatcga catcgcggtt gaagactgat ggctttcacc caccgcatca ccgcggttga
     1261 gcgcacccgc aggggccgct tttccatctt tgcggacggg gaatttctgt tttccgtcga
     1321 cgccgaaacg ctggcaaaaa gcggccttgc cgagggggac gaggtgtcgg aggccgagat
     1381 cgcggacctg cgcgaaaaca gcgacacccg ccgcgccaag gacaaggccc tcacctttct
     1441 cagcctgcgc gcctacggct cggcggagct gtacgacaag ctctgccgca gctttgacga
     1501 gcacaccgcg gctgcggccg tggccgagat ggcccgcctg ggcctgctgg acgacgcggc
     1561 attcgcccgc cgccgcgccg tctacctgca cggccagcgc aagagcctgt gggagatccg
     1621 ccaaaagctg ctcgccctgg ggctggaccg ggagctggtc gaggatgccc tggccgccct
     1681 gccgcaggac gagggcgggg ccatccgcgg ggtgatcgaa aagcaatacc tgtccaagct
     1741 ggcgcggggc gagacggaaa aggtcgtcgc ggcgctggcc cggcggggct tttccgcccg
     1801 cgaggcgcgg gccgccgtgg cggagttcct ctccgaccca gaaaccgccg gggattgagg
     1861 caaagggtgg ggtatcccgc ccttttcgtc tttcccctgc acaatacata acagaggatt
     1921 tttacatggc aactatcgca ttcatttctt tgggctgccc caagaaccag gtggacgccg
     1981 accgcatgtg ccacgcgctg ctggcggcgg ggcacctcac caccgccgac ccggccgagg
     2041 ccgacgtgat cgtggtcaac acctgcggct ttatcgagag cgccaagcag gaggccatcg
     2101 acaccatcct ggaggccgtg gagctgaaaa aaggcgacca tccccccaag gtggtggtca
     2161 ccggctgtct ggccgagcgc taccgggagg agatccaaaa agagatcccc gaggtggacg
     2221 ccacggtggg catcggctgc aacggcgaca tcgccgggct ggtggaccgg gtgctgcagg
     2281 gcgaaaagct gcaggcctac ggccccaagg caggcctgcc gctggagggc aagcgggtgg
     2341 tctccacccc gcgccactac gcctatctga agatcgccga gggctgcaac aaccgctgct
     2401 cctactgcgc catcccgctc atccgcgggc cgctgcgctc ccgcagcctg gacagcgtgg
     2461 tggaggaggc ccgctggctg gcctccgagg gggtcaagga gctgatcgtg gtggcccagg
     2521 atatcaccgc ctacggcgac gacctgggcc agaacctggc cgccccgctg ctggacgcgc
     2581 tgcagcaggt ggagggcatc cgctggatcc ggctgctcta cgcctatccc gagcgggtca
     2641 ccgacgcttt catcgccgcc atggcccgca acgacaaggt gctgccctat ctcgacctgc
     2701 ccatccagca catcaacagc gacatcctgc gctcgatgaa ccgcaagggc gaccgggaga
     2761 ccgtgctctc cgccattcgg cggctgcgcg ccggggtgcc ggggctgacc ctgcgcacca
     2821 ccctgatcgc gggctatccg ggcgagacgc aggcgcagtt cgacgagctg tgcgcctttg
     2881 tcaaggagac cgggttcgac cgcctgggct gctttgccta ctcggaggag gaggggacgc
     2941 cggccgcccg tatggagcag ctgccccaag agctgcggga acagcgggcc gagcgcatca
     3001 tggagatcca ggcggccgtg atggcggaaa agcaggccgc ccgccacggg gcgctgcttg
     3061 aggtgatctg cgacgggctg gacgaggaga cc
//
LOCUS       DBMDYA010000325         3083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7081_length_3083_cov_1.753856, whole genome shotgun sequence.
ACCESSION   DBMDYA010000325 DBMDYA010000000
VERSION     DBMDYA010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_3083_cov_1.753856"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(144..581)
                     /gene="rplO"
                     /locus_tag="ACZPFK_08445"
     CDS             complement(144..581)
                     /gene="rplO"
                     /locus_tag="ACZPFK_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR1406380.1"
                     /translation="MMLHELKPAEGASKAAKRKGRGAASGNGKTAGYGHKGQKARSGG
                     KKAGFEGGQMPLQRRLPKRGFNNIFATVYTTVKVSDLNRFEDGAVVDTAALLKSGLIK
                     KANDGVKVLGNGELTRKLTVEAAAFTASAKEKIEKAGGKAEVK"
     gene            complement(602..760)
                     /gene="rpmD"
                     /locus_tag="ACZPFK_08450"
     CDS             complement(602..760)
                     /gene="rpmD"
                     /locus_tag="ACZPFK_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR1406381.1"
                     /translation="MVRSLSGRKKDQIATAYSLGLRRVGHASMQPDNAATRGKINKIS
                     HLVEVCNA"
     gene            complement(1054..1581)
                     /gene="rpsE"
                     /locus_tag="ACZPFK_08455"
     CDS             complement(1054..1581)
                     /gene="rpsE"
                     /locus_tag="ACZPFK_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1406382.1"
                     /translation="MAFDRRQNPDRRQNPADQEYQEKVVAINRVSKTVKGGRVMKFSA
                     LVVVGDGKGKIGYGLGKAAEVPEAIRKGLEDAKKNMFTVSLKGTTIPHEVLGAFGAGR
                     VILRPAAAGTGLIAGGPVRAVLEVAGIRDIRTKCVRSNNPCNVVIATCNGLKSLRTAE
                     QVAATRGKTVKEILG"
     gene            complement(1600..1959)
                     /gene="rplR"
                     /locus_tag="ACZPFK_08460"
     CDS             complement(1600..1959)
                     /gene="rplR"
                     /locus_tag="ACZPFK_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1406383.1"
                     /translation="MVNKPNKNEARLRRHRRVRGKISGTASCPRLDVFRSNAHIYAQI
                     IDDVAGVTLAAASTMDKGFEGTGSNIEAAKKVGLAIAKAAKAKGIDKVVFDRGGYIYH
                     GRVKAVAEGAREGGLEL"
     gene            complement(1978..2523)
                     /gene="rplF"
                     /locus_tag="ACZPFK_08465"
     CDS             complement(1978..2523)
                     /gene="rplF"
                     /locus_tag="ACZPFK_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1406384.1"
                     /translation="MSRIGRKPIVIPAGVEVTINGSDVTVKGPKGTLHSTFHPMMKIE
                     KQGEEIVVTRPNDEKEARSLHGLTRTLVNNMVEGVSKGFTKELEIQGVGYRAAKQGKD
                     LVMNLGYSHQVVVSEEGDITIEVPDPNKIVIHGIDKQHVGQFAAQVREKRPPEPYKGK
                     GIRYVGEYVAHKEGKAGKGAK"
     gene            complement(2538..2936)
                     /gene="rpsH"
                     /locus_tag="ACZPFK_08470"
     CDS             complement(2538..2936)
                     /gene="rpsH"
                     /locus_tag="ACZPFK_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1406385.1"
                     /translation="MHITDPIADLLTRIRNASTAKHSTVDVPASNMKKAICQILLDEG
                     YIKGMQVKEDDKQGVITVTLKYGENNTPAITGLRRVSKPGLRIYKNCEELPKVMKGLG
                     TAIISTSKGVMTDKAARAANCGGEVLAFIW"
     gene            complement(2965..>3083)
                     /locus_tag="ACZPFK_08475"
     CDS             complement(2965..>3083)
                     /locus_tag="ACZPFK_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR1406386.1"
                     /translation="CKICGRPHAYLRKYGVCRICFRELAYKGEIPGVRKASW"
BASE COUNT          485 a          928 c          919 g          751 t
ORIGIN      
        1 ccatctcaga caggctgcct gggtccagga acgggacggg catggccacg ccgagacgga
       61 agatgacgat gatgatcaaa gtatagatca gctttttgcg cagatcctcg atcttccagg
      121 cgtttctcag tgttttaaac actttacttc acctcggcct ttccgccggc cttctcgatc
      181 ttctcctttg cggaggcggt gaaagcagcg gcctcgacgg tgagcttgcg ggtcagctcg
      241 ccgttgccga gaaccttgac gccatcgttg gcctttttga tgaggcccga cttgagcagc
      301 gcggcggtat cgaccacggc gccatcttca aagcggttga ggtcgctgac cttgacggtg
      361 gtgtaaacgg tggcaaaaat gttgttgaag ccgcgcttgg gcagacgacg ctgcaggggc
      421 atctggccgc cctcaaagcc cgctttcttg ccgccggacc gggctttctg gcccttgtgg
      481 ccgtagcccg cggtcttgcc gttaccggaa gccgcgcccc ggcccttgcg cttggcggcc
      541 ttgctggcgc cctcggcggg tttcagttca tggagcatca tagtagtaca cctccttgct
      601 tttacgcgtt gcacacctcg acgaggtggg aaatcttgtt gatcttgccg cgggtggccg
      661 cgttgtcggg ctgcatgctg gcatggccca cacggcgcag gcccagcgag taggcggtcg
      721 cgatctggtc ctttttgcgg ccggacaggc tgcgcaccag tttgacggtg acggtgcccg
      781 cggcggcgga tttcgcggcg cgcttcttgg cgggggtctt cgcctctgcg gcgccctcag
      841 ccttttcggc ggcttttttc gccgccggct tctttgccgg cgccttttcg gcagcatcct
      901 tcttgggggc ggcctttttg gcagcgggct tcttagcggg ggccttctcg gcggcttcct
      961 ttttgggggc agccttcttc gcaggcgcct tttcggccac ggcctcttcg gccacggcgg
     1021 tcactttctc ttctgccatg ctcaaaagcc tccttaacct aaaatctctt tgacggtctt
     1081 gccgcgggtg gcggcgacct gttcagcggt gcgcaggctc ttcaagccgt tgcaggtggc
     1141 gatgaccacg ttgcaggggt tgttggagcg cacgcatttg gtacggatat cgcggatgcc
     1201 cgcgacctcc agcaccgcgc gcaccgggcc gccggcgatc aggccggtac cggctgcggc
     1261 ggggcgcagg atcacgcggc ccgcaccgaa agcacccagc acctcatggg ggatggtggt
     1321 gcctttcagc gagacggtga acatattttt cttcgcatcc tcaagaccct tgcggatggc
     1381 ttcggggacc tcggcagcct tgccgagacc gtaaccgatc ttgcccttgc cgtcgcccac
     1441 gacgaccaga gcggagaact tcatcacgcg gccgcccttg acggttttgg aaacgcggtt
     1501 gatcgcgact actttctcct gatactcctg atccgcagga ttctgtcttc tatcgggatt
     1561 ctgtcttcta tcaaatgcca ctgttgtatc cctcctgctt tacagctcaa ggccgccctc
     1621 acgggcgccc tcggccaccg ctttcacgcg gccgtggtag atgtagccgc cgcggtcgaa
     1681 cacgactttg tcgatgccct tcgctttagc tgctttcgcg atcgcaaggc cgaccttttt
     1741 ggccgcctcg atattgctgc cggtgccctc aaagcccttg tccatggtgg acgcggcggc
     1801 cagggtcacg ccggcgacgt cgtcgatgat ctgggcatag atgtgcgcgt tagagcggaa
     1861 aacatcgagg cgaggacaag atgctgtgcc actgatctta ccgcggacgc ggcgatggcg
     1921 acgaagacgg gcttcgtttt tgttcggctt attgaccatc ttaattcact ccttcgttta
     1981 ttttgcgccc ttgccggcct tgccctcttt gtgagcgaca tactcgccga cgtagcggat
     2041 gcccttgccc ttatacggct cgggcggacg cttctcgcgc acctgcgcgg cgaactggcc
     2101 gacatgctgt ttatcaatgc catggatgac gatcttgttg ggatcaggga cctctatggt
     2161 gatgtcgccc tcttcggaga cgacgacctg gtgggaataa cccaggttca tgaccaggtc
     2221 tttcccctgc ttggcggcac ggtaaccgac gccctggatc tccagctcct tggtgaagcc
     2281 cttggagacg ccctcaacca tgttgttgac cagagtacgg gtcaggccgt gcagagagcg
     2341 agcctctttt tcatcgttgg gacgggtcac gacgatctct tcaccctgct tttcgatctt
     2401 catcatcggg tgaaaagtgg aatgcagcgt gcccttgggg cccttgacgg taacgtcgct
     2461 gccgttaatc gtgacctcga cgcccgcggg aatgacgatg ggttttcttc caattctcga
     2521 cattgtcttg caccccctta ccagataaag gcgaggactt cgccgccgca gtttgctgcg
     2581 cgcgcggcct tgtcggtcat gacgcctttg gatgtggaaa tgatcgcggt accaaggcct
     2641 ttcatgacct tgggcagttc ctcacagttc ttgtagatgc gcaggccggg tttcgatacc
     2701 cggcgcagcc cggtgatggc cggggtgttg ttctcgccgt atttcagggt gacggtgatg
     2761 acaccctgct tgtcgtcttc ttttacctgc atgcctttga tgtagccctc gtccaacagg
     2821 atctggcaga tcgccttctt catgttggaa gccggtacat ctactgtaga gtgctttgca
     2881 gtgctggcat tccgaatacg ggtgagcaaa tcagcgatgg ggtcggtgat atgcatgcca
     2941 tgtacctcct ttatcgtaga gtttttacca acttgctttc cttacgccgg ggatctcgcc
     3001 cttataggcc agctccctga agcagatacg gcacacgccg tacttgcgca gataagcgtg
     3061 agggcggccg cagatcttgc agc
//
LOCUS       DBMDYA010000326         3068 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7130_length_3068_cov_1.642400, whole genome shotgun sequence.
ACCESSION   DBMDYA010000326 DBMDYA010000000
VERSION     DBMDYA010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7130_length_3068_cov_1.642400"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(79..1680)
                     /locus_tag="ACZPFK_08480"
     CDS             complement(79..1680)
                     /locus_tag="ACZPFK_08480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1406387.1"
                     /translation="MATVIYNAKVYVERGNFAQAVLVEDGLIRAVGTNEEVLAAAPAD
                     AERYDAAGRTVVPGFNDSHQHLYSVGTNLESVDVHGVTSIAEVKRITREFIAKHQPAP
                     GSVVYGSGWNQDYFTDEARLLTRQDLDEVSTDLAIVLERACGHILTANTRAIELAGIT
                     KASAPVAGGAFDYDADGELTGVFRENACNAFTAIKAEQTVEMVERTLRTAMAHAAECG
                     VTSVQTMDVRPSDWPIMLEAYKRVQQDPTVRVYHQCNFQDPESFQRFLDAGYKTGVGD
                     AVNKIGPLKLFVDGSLGARTALMREPYRDDPATSGIATLTQQEFDTLVGMAAEHGCQV
                     VTHAIGDGAIELVLNGYDTVCRDGKNPLRLGVVHCQITDRPLVERFTENDILALVQPI
                     FLHYDMTVLESRVGKELASTSYAFHTMNKLGIHTSFGTDSPVEDLNTMNNLYCAVARK
                     NLDKKPEGGFYPHECMDIYEAVDGYTAGSAYASFEEGVKGRIKPGYYGDLAILSQDIF
                     TIPTDELLTTVVDATVLGGEFVYER"
     gene            complement(1682..>3068)
                     /locus_tag="ACZPFK_08485"
     CDS             complement(1682..>3068)
                     /locus_tag="ACZPFK_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1406388.1"
                     /translation="SSEKTELKREVGLFGGISVLAGIMIGSGIFYIGGIVLQRAGMSL
                     GLALLVWVVGGLITLMSGICYAELGAMMPKAGGGYVYLREAYGERVAFMSGFSNFILG
                     SSGSNAALAVAFSTSIASMVPGSFFAESGLTQKGLAIGMIVLLTVVNIFGIKLGSTVQ
                     NIFMVLKMLPIFAILVCGLFMGKQNPSLSIIPTSNPGFAQICGMVAFGVVATMWAYEG
                     WTNLNGIAEEIKKPKKNLPLAIIVSIIGVTVLYVLFNYAVYRVLPYDTIVSMIESGNY
                     YLGTAAAGSLFGNAGGLIVGAAMILAIFNSLNGCVMVFPRTYYAMARDGAFFKSMAKL
                     HPTYKTPVNALVVSGAVSIALVCARDLGQLTSLVAFCGMVFNGLTFYAVIRLRKKYPT
                     MDRPYKVWGYPVMIILICLIMIGLMINTFMEDPVTSIIGLAVPIVGLVLYELVFKKNR
                     EAVIRGEKEEK"
BASE COUNT          550 a          946 c         1020 g          552 t
ORIGIN      
        1 cgagcagaag aaaaagagcg ccggaacagg gaagacggcc ctgttccggc gctctctttt
       61 ttgcggggga agcgcgatct agcgctcgta gacgaactcg ccgccgagga cggtggcgtc
      121 caccacggtg gtgagcagct cgtcggtggg gatggtgaag atgtcctggc tgaggatggc
      181 caggtcgccg tagtagccgg gcttgatgcg gcccttcacg ccctcctcaa agctggcgta
      241 ggcgctgccg gcggtgtagc cgtccacggc ctcgtagatg tccatgcact cgtgggggta
      301 gaagccgccc tccggctttt tgtccaggtt tttgcgggcc acggcgcagt agaggttgtt
      361 catggtgttc aggtcctcca ccgggctgtc ggtgccaaag gaggtgtgga tgcccagctt
      421 gttcatggtg tggaaggcgt agctggtaga ggccagctcc ttcccgaccc ggctctccag
      481 caccgtcatg tcgtagtgca ggaagatggg ctgcaccagc gccaggatat cgttctcggt
      541 gaaccgctcc accagcgggc ggtcggtgat ctggcagtgc accacgccca ggcgcagcgg
      601 gtttttgccg tccctgcaga ccgtgtcgta gccgttgagc accagctcaa tggcgccgtc
      661 gccgatggcg tgggtcacca cctggcagcc gtgctcggcc gccatgccca ccagggtgtc
      721 aaactcctgc tgggtgaggg tggcgatgcc gctggtggcg gggtcgtccc ggtagggctc
      781 ccgcatcagg gcggtgcgtg cgcccaggct gccgtccaca aacagcttca gcggcccgat
      841 cttgttcacc gcgtcgccga cgccggtctt ataccccgcg tccagaaagc gctgaaagct
      901 ctccggatcc tggaaattgc actgatgata gacgcggacg gtggggtcct gctgcacccg
      961 cttgtaggcc tcgagcatga tgggccagtc ggaggggcgc acgtccatgg tctgcaccga
     1021 ggtcacgccg cactcggccg cgtgggccat ggcggtgcgc agcgtgcgct ccaccatctc
     1081 caccgtctgc tcggccttga tggcggtaaa ggcgttgcag gcgttctcgc ggaacacgcc
     1141 ggtcagctcg ccgtcggcgt catagtcaaa ggcgccgccc gccacgggcg ccgaggcctt
     1201 ggtgatgccc gccagctcga tggcgcgggt gttggcggtg aggatgtggc cgcaggcgcg
     1261 ctccagcacg atggccaggt cggtggacac ctcgtccagg tcctgccggg tgagcaggcg
     1321 ggcctcatcg gtgaaatagt cctggttcca gccgctgccg tagaccacgc tgccgggggc
     1381 gggctgatgc ttggcgatga attcgcgggt gatgcgcttc acctccgcga tgctggtgac
     1441 gccgtgcaca tccaccgatt ccaggttggt gcccaccgaa tacagatgct ggtgggaatc
     1501 gttgaagccc ggcaccacgg tgcgccccgc ggcatcgtac cgctcggcgt ccgccggcgc
     1561 ggcggcaagg acctcctcat tggtgcccac ggcccggatc aagccgtcct ccaccagcac
     1621 cgcctgggca aaattgcccc gctccacata gacctttgcg ttataaatga cagttgccat
     1681 cttacttttc ctccttttcg ccgcggatga cggcctcgcg gttctttttg aataccagct
     1741 cgtaaagcac cagccccaca atgggcaccg ccaggccgat gatcgaggtg acggggtcct
     1801 ccataaaggt gttgatcatc aggccgatca tgatcaggca gatcaggata atcatgacgg
     1861 gatagcccca gaccttgtag gggcggtcca tggtgggata ttttttgcgc agccggatga
     1921 ccgcgtagaa cgtgagcccg ttgaagacca tgccgcagaa cgccaccagg ctggtgagct
     1981 ggcccagatc gcgggcgcag accagggcga tggagacggc gccggacacc accagcgcgt
     2041 tgaccggggt cttgtaggtg gggtgcagct tggccatgct cttgaagaaa gcgccgtcgc
     2101 gggccatggc gtaataggtg cgcggaaaaa ccatgacgca gccgttgagc gagttgaaga
     2161 tggccaggat catggccgcg cccacgatga ggccgcccgc attgccgaac aggctgcccg
     2221 cggccgcggt gcccagataa tagttgccgc tctcgatcat cgagacgatg gtgtcgtacg
     2281 gcagcacccg gtagaccgca tagttgaaca gaacatagag cacggtgacg ccgatgatgg
     2341 agacgatgat ggccaggggc aggttctttt tcggcttttt gatctcctcg gcgatgccgt
     2401 tcaggttggt ccagccctca taggcccaca tggtggccac cacgccgaac gccaccatgc
     2461 cgcagatctg ggcaaagccg gggttcgagg tcgggatgat cgagaggctg gggttctgct
     2521 tgcccatgaa caggccgcag acgaggatgg cgaagatggg cagcatcttc agcaccataa
     2581 agatgttctg caccgtgctg cccagcttga tgccgaagat gttgaccacg gtcagcagca
     2641 cgatcatgcc gatggccagc cccttttggg tcaggccgct ctcggcgaaa aagctgccgg
     2701 gcaccatgct ggcgatggag gtggaaaagg ccaccgccag cgccgcgttg gagccggaag
     2761 agccgaggat gaagttggaa aagccgctca taaaggcgac ccgctcgccg taggcctcgc
     2821 gcaggtagac gtagccgccg cccgccttgg gcatcatggc gcccagctcg gcgtagcaga
     2881 tgccgctcat cagggtgatg aggccgccta ccacccacac cagcagcgcc aggcccaggc
     2941 tcatgcccgc acgctgcagc acgatgccgc cgatgtagaa gatgccggag ccgatcatga
     3001 tgcccgccag caccgagatg cccccgaaca ggcccacctc gcgcttgagc tcggtctttt
     3061 cggatgag
//
LOCUS       DBMDYA010000327         3061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7153_length_3061_cov_1.589527, whole genome shotgun sequence.
ACCESSION   DBMDYA010000327 DBMDYA010000000
VERSION     DBMDYA010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7153_length_3061_cov_1.589527"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(56..988)
                     /locus_tag="ACZPFK_08490"
     CDS             complement(56..988)
                     /locus_tag="ACZPFK_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406389.1"
                     /translation="MKIDRMIGILAILLQREQVTAPELAARFEVSRRTISRDIDALAR
                     AGVPVTTSQGAGGGISVMPGYRVDRTLLTRPELSAILAGLAGLDSVSESPRYRQLMEK
                     LDAGGGPDEALRIDLASYYRASLAPKLELLRQAIAQRRPVGFRYAGPSGEGPRRVEPG
                     QLLFEWSSWYLRGWCRDRQAFRTFKLARMEGLKLLEGHFLSRPLPEDGGFDPARAFPA
                     RVCILAAFDPSVRWRLLEEYGADQLEERDGRLLLRAWFPDEDYPLGWLLSFGDAAELL
                     EPAALRAKLAALARRIAARHEAEAAPPEPAAPAP"
     gene            1858..2988
                     /locus_tag="ACZPFK_08495"
     CDS             1858..2988
                     /locus_tag="ACZPFK_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406390.1"
                     /translation="MIEYKNVCKNYGDRRIVKDLSFTVEDGQFAVLIGPSGCGKTTTL
                     KMLNRLIQSDGGEILLDGKNVNDINAEELRTHIGYVIQQIGLFPNMTVEQNITVVPRL
                     LKWDKERCHKRVRELLALVDMDYDTYAKKYPNELSGGQQQRVGVLRALAAEPPVILID
                     EPFGALDPITRDALQDEVKNLQKRLHKTIIFVTHDMNEAIKMADVILFMSDGELLQQA
                     SPEEMLRAPANDTVREFMGKHVAQGVAGQDNIPCGEIMRKNVAKVSDTWRTLECVQLM
                     SSREIDSLVVVDDSDRYLGVVTIEGIKARGKAGASVRNILETTVPTVRTDTLAKEAFA
                     TLDSTKARYLVVLDKDDRAVGIITRTSMSKALAYMVWGGDAV"
BASE COUNT          599 a          979 c         1005 g          478 t
ORIGIN      
        1 gcaaacagcg ccgttccggg cacggaaagc cctttgcggg cggggcgggc gcggctcagg
       61 gcgcgggcgc tgcgggctcc ggtggggcgg cttccgcctc gtggcgggcg gcgatgcggc
      121 gggccagcgc ggccagcttc gcccgcaggg cggcgggttc cagcagctcg gccgcgtccc
      181 caaagctgag cagccagccc agcgggtaat cctcatccgg gaaccaggcg cgcagcagca
      241 gcctgccgtc ccgctcctcc agctggtccg ccccgtactc ctccagcagc cgccagcgca
      301 ccgacgggtc gaaagccgcc aggatgcaga cccgggcggg aaaggcgcgc gcagggtcaa
      361 agccgccgtc ctcgggcagg ggacgggaga gaaaatggcc ctccagcagc ttcagcccct
      421 ccatgcgggc cagcttgaac gtgcggaacg cctgccggtc ccggcaccag ccgcgcagat
      481 accagctgga ccactcaaac agcagctgcc ccggctctac ccggcgcggg ccctcgcccg
      541 agggcccggc atagcgaaag cccaccggcc gccgctgggc gatggcctgc cgcagcagct
      601 ccagcttggg ggccagggag gcgcggtaat agctggcaag gtcgatgcgc agcgcctcgt
      661 ccggcccgcc gcccgcgtcc agcttttcca tcagctggcg gtaacggggg ctttcggaca
      721 cgctgtccag ccccgccagc ccggccagga tggcggacag ctcgggccgg gtgagcaggg
      781 tgcggtccac ccggtagccg ggcatgaccg agatgccccc gcccgccccc tggctggtgg
      841 tcaccggcac ccctgcccgc gccagcgcgt cgatgtcccg gctgatggta cgccgggaga
      901 cctcaaagcg ggcggccagc tccggcgcgg tcacctgctc ccgctgcagc aggatagcca
      961 ggatgccgat catccggtcg atcttcatcc gccgtccctc ccctcgatga tcttgttccc
     1021 cctctatcat accacggcgg ggcgcgcggg caaatggaaa ttgcaggccc ggagagcagg
     1081 cgcggctctc cccatccgcc tgcggctgcc gcccgatggc cgcctgaaag cgcgggcccg
     1141 gccaggagaa agcggccttg gccgacatgc ccccgctgcg gaggcggcgg cagcgggggc
     1201 atgtcggcag aaaaagggcg cggcggcagg gggacagaag cagcctcact ttcagctggt
     1261 atcgcctgcc ccggcccgcc gcgggggaag agagccccct gccgggcttt gcctccgcgc
     1321 cgtccgcgcg gcgcccgcag gggaaaagcg gggccggggc ggatcgtttg gccgtcggcc
     1381 ggtccatcgg cgcgcggcgg ggaaaatatg aagaaaatat taaatttaca gtttcttttt
     1441 tccaaggggc cgcacccact tgacaaagtg acaacttcaa gtgtattata aagttgttat
     1501 aaaaaatagc cgcaggctat tttttcggcc atgcggcccg tcctcgggcc gcggccattg
     1561 aaatcagttg ttttttattg cggagcaata aaaaatagcc gcaggctatt ttttcggcca
     1621 tgcggcccgt cccctggccg gggccataga aatcagttgt ttttttattg caaagcaata
     1681 aaaaatagat gcccccagtc gaaagttgag gccggggcga ttctgttttt tgtacaaaaa
     1741 agcagaagaa aaaacatttt ttttgacctc attgcccaga ggtgctataa tagcaggaaa
     1801 aaaatggaga ttacaggcag ggccgcgccc tgttgaacaa gcgggaggca gaaaaggatg
     1861 atcgaataca aaaacgtctg caagaactac ggcgaccgca ggatcgtcaa ggacctcagc
     1921 ttcacggtgg aggacgggca attcgcggtg ctgatcggcc cgtccggctg cggcaagacc
     1981 accaccctga agatgctcaa ccggctcatc cagtcggacg gcggcgagat tttgctggac
     2041 ggcaaaaacg tcaacgacat caacgccgag gagctgcgca cccacatcgg ctatgtgatc
     2101 cagcagatcg gcctgttccc caacatgacg gtggagcaga acatcaccgt ggtgcccagg
     2161 ctgctgaagt gggacaagga gcgctgccac aagcgggtgc gtgagctgct ggccctggtg
     2221 gacatggact atgatactta tgccaaaaaa tatcccaacg agctgtcggg cgggcagcag
     2281 cagcgggtgg gcgtgctgcg cgcgctggcg gccgagccgc cggtcatttt gatcgacgaa
     2341 ccgttcggcg cgctggaccc catcacccgc gacgcgctgc aggacgaggt caaaaacctg
     2401 caaaagcggc tgcacaagac catcatcttc gtcacccacg acatgaacga ggccatcaag
     2461 atggcggacg tgatcctgtt catgagcgac ggcgagctgc tgcagcaggc ctcccccgag
     2521 gagatgctgc gcgccccggc caacgacacg gtgcgcgagt tcatgggcaa gcatgtggcg
     2581 cagggcgtgg ccgggcagga caacatcccc tgcggcgaga tcatgcgcaa aaacgtggcc
     2641 aaggtatccg acacctggcg cacgctggag tgcgtgcagc tgatgagcag ccgcgagatc
     2701 gactcgctgg tggtggtgga cgacagcgac cgctatctgg gcgtggtcac catcgagggc
     2761 atcaaggcgc gcggcaaggc gggcgcctcg gtgcgcaaca ttttggagac cacggtgccc
     2821 accgtgcgca ccgacacgct ggccaaggag gcctttgcca cgctggacag caccaaggcc
     2881 cgctacctgg tggtgctgga caaggacgac cgcgccgtgg gcatcatcac ccgcaccagc
     2941 atgtcgaaag ccctggccta catggtctgg ggaggtgacg ccgtatgacc cacttttttg
     3001 aggcttacgg cctgatgctg gtgaaagcca tcttcgtgca tctggcctat gtgctcgcct
     3061 c
//
LOCUS       DBMDYA010000328         3058 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7163_length_3058_cov_1.827866, whole genome shotgun sequence.
ACCESSION   DBMDYA010000328 DBMDYA010000000
VERSION     DBMDYA010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_3058_cov_1.827866"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="ACZPFK_08500"
     CDS             <1..286
                     /locus_tag="ACZPFK_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406391.1"
                     /translation="IAFLGTAAELTRQNSAAARLRVRFSAAPELQGCRACRLCGEEHG
                     EMIFEAPDLTAALAELAALASQQEAQILDVATEHASVEERFLDIAREGTA"
     gene            283..999
                     /locus_tag="ACZPFK_08505"
     CDS             283..999
                     /locus_tag="ACZPFK_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406392.1"
                     /translation="MSAFLYGVGLHWRMDLRNREVVIVYYVVPLIFFLFMGGFFSAVM
                     PGAEQTLIQSMTVFGATMGAMIGSPAALAETYNSETRKAYRVGGVPLWTAVAANLLSS
                     FLHLLLMSAILYLAAPLLYGAVPPPHPAAFFAALALFLFCSLCVGTLLGLLASTPSRL
                     TMLSQLLFLPSVLVSGIMVPAAMLPRALQLAGKLLPATWGYELLCAASFQLRPVLALL
                     GMAALCLALCGWRLKKIYSE"
     gene            1183..1536
                     /locus_tag="ACZPFK_08510"
     CDS             1183..1536
                     /locus_tag="ACZPFK_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406393.1"
                     /translation="MEEFKPGPEKALNCKRHRVERPTMLFCEQTKLKYSRLPEEEKRE
                     LARIAQKSQFIKGPVSQWGKKRWPDRRKPRFMVDYGQDGPELRTTTGLLTDQGPQKTQ
                     AIFSADETKNAGKPL"
     gene            1690..2229
                     /locus_tag="ACZPFK_08515"
     CDS             1690..2229
                     /locus_tag="ACZPFK_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1406394.1"
                     /translation="MGIGIVVCGLNGAGKSTLGKALAEKLNFYFVDSEDLYFPKTDSG
                     YLYTSPRTHNEAKKLFLDEMKEHENFVFASVKGDYGGDNLSFFQYAVLVDVPKDIRVQ
                     RVKNRSFQKFGNRMLPGGDLHEQEELFFDFVKSRAEDVVEKWAQSLPCPILRVDGTKP
                     FEENVNFIMSRIQKQFSVL"
     gene            complement(2378..>3058)
                     /locus_tag="ACZPFK_08520"
     CDS             complement(2378..>3058)
                     /locus_tag="ACZPFK_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1406395.1"
                     /translation="LLSNYMTGRDGNRGQCAQPCRWKYALVEEKRPGEYFEIGENEEG
                     SYILSAEDLCAAPFLGRVAAAGVDSFKIEGRAKSFYYVASVTAAYRRAIDAIGGDAAA
                     YRCPPEALAELEKSSHRRYSPGFYLGRDLAVQNVTDDRYQRGWQIVAVVERCEAGVAA
                     CTQRGKFELGEAVEALLPDGTVVPFAPAWLRDAEGQPITGTPHAMMAFTLPVPRPLPP
                     GSILRKKK"
BASE COUNT          649 a          869 c          903 g          637 t
ORIGIN      
        1 gattgccttt ctcggcacgg cggccgagct gacccggcag aacagcgcgg ccgcgcggct
       61 gcgggtgcgt ttttccgccg cgccggagct gcagggctgc cgcgcctgcc gcctttgcgg
      121 cgaagagcac ggggaaatga tttttgaagc cccggacctg acggccgccc tggccgagct
      181 ggcggcgctg gcctcgcaac aggaagccca aattctggat gtggccaccg aacacgccag
      241 tgtggaagag cgttttctgg atattgcaag ggaggggacg gcatgagcgc ctttttatac
      301 ggcgtcggtt tgcactggcg catggacctg cgcaaccgcg aggtcgtgat cgtctactat
      361 gtggtgccgc tgatcttttt tctctttatg ggcggcttct tcagcgccgt gatgccgggc
      421 gcggagcaga ccctgatcca gtcgatgacg gtgttcgggg ccaccatggg cgccatgatc
      481 ggcagcccgg ccgccctggc cgagacctac aacagcgaga cgcgcaaggc ttaccgcgtg
      541 ggcggcgtgc ccctttggac ggccgtcgcc gccaatctgc tctcctcttt tctgcatctg
      601 ctgctcatga gcgccatcct ttatctggcc gccccgctgc tatacggcgc ggttccgccg
      661 ccgcacccgg ccgcgttttt cgccgctctg gcgctgttcc tgttttgcag cctctgtgtg
      721 ggcacccttt tgggcctgtt ggcttccacc ccgtcccggc tgaccatgct ctcacagctt
      781 ctctttctgc cctcggtgct ggtgtccggc atcatggtac cggcggcgat gctgccccgc
      841 gctttgcagt tggcgggcaa gctgctgccc gccacctggg gctacgagct gctctgcgct
      901 gcaagctttc agctgcggcc tgtgctggcc ctgctgggta tggccgcgct ctgcctggct
      961 ctgtgcggct ggcgcctcaa aaaaatttac agcgagtgag atcggccgca gacaaaaaag
     1021 gaggagcccg tcttaacgtg cccccaataa aaaaatattg gcacggcaag cggcatagtc
     1081 cataggacta tgccgcttgc cgtgcatgtc gttggggcga tgatggagga tatcagaaac
     1141 cctctccgag atcggggcga gcgtacccgg gagccctcca ttgtggagga attcaagccg
     1201 ggcccggaaa aggcgttgaa ctgcaaaaga caccgtgtag aaaggccgac gatgcttttc
     1261 tgcgagcaga caaagttaaa atacagccgc ttgccggagg aagaaaaacg agagcttgcc
     1321 cgtatcgcac agaaatctca attcatcaaa ggccccgttt cacagtgggg aaagaagcgg
     1381 tggccagata gacgaaaacc ccggtttatg gtagactacg ggcaagacgg tcctgagctt
     1441 cggaccacaa cggggctgtt gacagaccag ggaccgcaga aaacgcaggc gattttttcg
     1501 gcagatgaaa caaaaaacgc aggcaagcct ttgtgactta acgagtatct ttgtgaaata
     1561 tgccgcaaca aagcgccaat gttttcaagg agtgactgtg tcaacagccc cacaacgatc
     1621 caaagtcaga tgtgcgcctt tcgcggggaa aacacatttg acaaattggg agctgtaagg
     1681 ggaaaatgta tgggaatagg aattgtggta tgcgggttaa atggagccgg aaaaagcacc
     1741 ttggggaagg cccttgcgga aaaattaaat ttttattttg tcgatagtga ggatttgtac
     1801 tttccaaaga cggactccgg ttatctatac acttcccctc gcacccataa tgaagcaaaa
     1861 aagctctttc tcgatgagat gaaagaacac gaaaactttg tttttgcttc tgtcaaaggg
     1921 gattatgggg gagacaatct atcctttttt caatatgccg tattggttga cgtcccgaaa
     1981 gatattcggg tacaacgagt taaaaatcgc tcttttcaga aattcgggaa cagaatgctg
     2041 ccgggcggag atttacatga gcaagaggag ctcttctttg attttgttaa atccagagca
     2101 gaagatgtcg ttgaaaaatg ggcgcagtct ctgccctgcc ctatcctacg agtagacggg
     2161 acaaaaccct ttgaagaaaa tgtaaatttt atcatgagcc ggatacaaaa acaattttca
     2221 gttttataga gctgtttgag tatactttct tggtagaaag gacaggcaac aaaaacggaa
     2281 aaatcctcgc agggctttcc cggcccacgg ccttaaaatt ccaggcgccc gcccatagga
     2341 atgggcggat gcgcataaaa aacagccgca gcggcgacta tttctttttg cgcaggatcg
     2401 accccggcgg cagaggcctg ggcacgggca gggtgaacgc catcatggcg tggggcgtgc
     2461 cggtgatggg ctgcccctcc gcatcccgca gccaggccgg ggcaaagggc accaccgtgc
     2521 cgtccggcag cagggcctcg acggcctctc ccagttcgaa ttttccgcgc tgggtacagg
     2581 ccgcgacccc ggcctcgcag cgctccacca ccgcaacgat ctgccagccc cgctgatagc
     2641 ggtcgtcggt gacgttctgt acagcgaggt cccgtcccag ataaaaaccg ggcgaatagc
     2701 gccgatggct ggatttttcc agctccgcca gcgcctcggg cgggcagcgg taggcggcgg
     2761 cgtccccgcc gatggcgtcg atggcccgcc ggtaggcggc cgtcacgctg gccacatagt
     2821 aaaagctctt ggcccggccc tcgatcttga agctgtccac cccggcggcg gccaccctgc
     2881 cgagaaaagg ggccgcacag aggtcctcgg cgctgaggat atagctgccc tcttcattct
     2941 cgccgatctc aaagtactcg ccggggcgct tttcctccac cagggcgtac ttccaccggc
     3001 agggctgggc gcactggccc cggttgccgt cccgtccggt catatagttg gacagcag
//
LOCUS       DBMDYA010000329         3056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7169_length_3056_cov_1.462267, whole genome shotgun sequence.
ACCESSION   DBMDYA010000329 DBMDYA010000000
VERSION     DBMDYA010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7169_length_3056_cov_1.462267"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="ACZPFK_08525"
     CDS             complement(<1..217)
                     /locus_tag="ACZPFK_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1406396.1"
                     /translation="MDCSAEKEVDVRPFAYAISLIEGKWKMHILFWLWKAEVLRYSEL
                     KRSLGRVTHKMLSAQLKELEADGVILRR"
     gene            403..1398
                     /locus_tag="ACZPFK_08530"
     CDS             403..1398
                     /locus_tag="ACZPFK_08530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR1406397.1"
                     /translation="MQYLNQPLVLQGMRLNNRLVMPPMATAKSTAEGAVTQALCEYYR
                     ARAEGGFLGLVITEHSYVSAQGKASAGQLSAADDGAVEGLRELVAAARRDGARVMAQI
                     NHAGAAADPAVTGCEAVAPSAAARPQARADLPLPRALTKGEIADIAAQFAQAARRVQQ
                     AGFDGVEIHAAHGYLLNQFYSPLTNFRDDEYGADCIENRVRFHLQVIEAVRGAVGPDY
                     PVALRFGGCDYHAGGSTVEDSVRACALFERAGVDLLDISGGMNGYRIPGLEGPGYFRE
                     MTAAIKQAVLAPVLLTGGVTAAAEAEQLLAQGAADLIGVGRPLLADPRWAEKAFA"
     gene            complement(1505..2077)
                     /locus_tag="ACZPFK_08535"
     CDS             complement(1505..2077)
                     /locus_tag="ACZPFK_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406398.1"
                     /translation="MKYLPNHFVKKAVLVCILLVAAALFGWLFHRRYEIVERAPQPVG
                     APVFPSVDFVLASPIEIRGLNCDMISVDRAGNLYLANPRFMTTFLSSIAEGSAHLLED
                     KKISVYLIKSTRPSGTVSGPEITFVVQYETGELVSSEVIECEGETILMPESEQKKWGV
                     VLASFLLTAQNLYAYVDGHPTMLAATPAPP"
     gene            complement(2096..2977)
                     /locus_tag="ACZPFK_08540"
     CDS             complement(2096..2977)
                     /locus_tag="ACZPFK_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1406399.1"
                     /translation="MHFTDAKGILSPSNGMNLYRGCSHGCIYCDSRSKCYHMEHPFED
                     IEVKANAIELLESALKRKRSKCMLGTGSMTDPYIPLEEEMGNVRKALSLAHQYGFGFT
                     LITKSTRVLRDLDLLKAINDQTKCVVQMTLTTCDEALCKKIEPNVSTTRERFEALKQL
                     QRAGIPTVVWLCPILPFINDTAENIRGILQYCREARVYGIICFGMGLTLREGSREYFY
                     SQLDRLFPRMKEKYIRAYGMQYHLDSPKSNELMALFHRLCEEGGIAHDNKQIFAYLNR
                     FEEKNPPVQLSLSDETL"
BASE COUNT          585 a          899 c          940 g          632 t
ORIGIN      
        1 cccggcgcag gatcaccccg tccgcctcca gctctttcag ctgggcgctc agcattttgt
       61 gggtcacccg ccccaggctg cgcttcagct cgctgtagcg cagcacctcg gctttccaga
      121 gccagaacag gatatgcatt ttccacttgc cctcgatgag cgagatggca taggcaaagg
      181 gacggacgtc cacctctttt tcggcgctgc agtccatatt gctctccttt ttgatagtat
      241 ctacccaaaa agtgcatact tgtcattctg aaaggtattt gccataataa tagcaaagag
      301 cgcaggcgtt tgcaaatcgc cccggcgtcc ccgcaaaaaa accggggcgg gcgcggcggc
      361 gcgtccgcgt tggaaagata aaaaagaaac ggaagtggtt tcatgcagta tctgaaccag
      421 ccgctcgtcc tccaggggat gcggctgaat aaccgcctcg tcatgccgcc gatggcgacc
      481 gccaagtcga cggcggaggg cgcggtcacc caggcgctct gcgagtatta ccgggcgcgg
      541 gccgaggggg gctttctcgg ccttgtcatc accgagcaca gctatgtgag cgcgcagggc
      601 aaggccagcg ccgggcagct gtcggcggcc gacgacggcg ctgtggaggg cctgcgggaa
      661 ttggtcgcgg ccgcgcgccg ggacggcgcc cgcgtgatgg cgcagatcaa ccacgccggc
      721 gccgccgccg acccggcggt gaccgggtgt gaggcggtgg cgcccagcgc ggccgcccgc
      781 ccgcaggcca gagcggatct gcccctgccc cgcgcgctca caaaagggga gatcgccgac
      841 atcgccgccc agttcgccca ggcggcccgc cgcgtgcaac aggcgggctt tgacggcgtg
      901 gaaatccacg ccgcgcacgg gtatctgctg aaccaatttt attcgccgct gaccaatttt
      961 cgcgatgacg agtacggggc ggattgcatc gaaaaccggg tgcggttcca tctgcaggtc
     1021 atcgaggcgg tgcgcggggc cgtggggccg gattatccgg tggcgctgcg ttttggcggc
     1081 tgcgattacc acgccggggg cagcacggtg gaggacagcg tgcgggcctg cgcgctcttt
     1141 gagcgggccg gggtggacct gctggatatt tccggcggca tgaacggcta ccgcatcccg
     1201 gggctggagg ggccgggcta tttccgcgag atgaccgccg cgataaaaca ggcggtcttg
     1261 gcgccggtgc tgctgaccgg cggcgtgacg gcggcggcgg aggccgagca gctgctggcg
     1321 cagggggccg ccgacctgat cggcgtgggc cgcccgctgc tggcggaccc gcgctgggcg
     1381 gaaaaggcgt ttgcctgagc ggcttcccgt accgccccca cagcgcccgc cgtaaaaaag
     1441 gagagccgcc gcagagcttt ttctgcggcg gctctttttg tgctttgacg ttcggcaaga
     1501 cgggtcacgg cggggccggc gtcgccgcca gcatggtggg gtggccatcc acataggcgt
     1561 agaggttttg ggcggtgaga agaaaggagg ccaggaccac gccccatttt ttctgctcgc
     1621 tctcgggcat gaggatggtc tccccctcgc actcgatcac ctcgctggac accagctccc
     1681 cggtctcgta ctggacgaca aaggtgatct cggggccgct gaccgtgccc gacggccggg
     1741 tggatttgat gaggtagacg ctgatttttt tgtcctccag aagatgggcg cttccctccg
     1801 cgatgctgct caaaaaggtg gtcataaagc gggggttggc gagatacagg ttcccggccc
     1861 ggtccaccga gatcatgtcg cagttcagcc ccctgatctc gatgggcgag gcgagcacaa
     1921 aatccaccga ggggaacacc ggcgcgccca ccggctgcgg ggcccgctcg acaatttcat
     1981 accgccggtg gaacagccac ccgaacaggg cggccgcgac aagcaggatg cacaccagga
     2041 cggctttttt gacgaaatgg tttggcaaat atttcatttt tgttcagcga ttgtgttaga
     2101 gggtttcatc ggataggctc aactgaaccg ggggattctt ctcttcaaat ctgttcagat
     2161 acgcaaatat ctgcttgttg tcatgggcga ttccgccctc ctcgcacagc cgatggaaca
     2221 atgccatcag ctcgttgctc ttggggctgt ccaggtgata ctgcatgcca taagcgcgga
     2281 tatatttttc tttcatccgc ggaaacagcc ggtccaactg gctgtaaaag tattcccggc
     2341 ttccctcgcg aagcgtcaac cccattccaa aacagatgat accataaacc cgcgcctccc
     2401 tgcaatattg cagaataccg cggatgtttt ccgccgtgtc gttgataaag gggagaatgg
     2461 ggcagagcca gacaacggtg ggaatacccg ccctttgaag ctgctttaat gcctcaaagc
     2521 gctctctcgt cgtactgacg ttcggctcga tttttttgca tagcgcttca tcgcaggttg
     2581 tcagcgtcat ctgtacgacg cattttgttt gatcgttgat cgctttcagc aaatctaaat
     2641 cccgcagcac ccgagttgac tttgtgatca gagtgaaccc aaagccgtat tgatgggcca
     2701 gggataacgc ttttcggaca ttccccatct cctcttcaag cggtatatag gggtctgtca
     2761 tggagcctgt ccccaacatg catttggagc gtttccgttt cagggcgctt tccaaaagct
     2821 caatggcgtt tgctttaact tcaatatcct caaaaggatg ctccatgtga taacacttgc
     2881 ttctggaatc acagtagatg cacccatggg agcagccgcg atacaggttc atgccgtttg
     2941 agggggataa aatccctttt gcgtccgtaa agtgcataag gcttcctcta tcaatcaaat
     3001 tcatccgtac acgggagcgg ttgttgtctt cacgttacag ccgatacggc gtcgat
//
LOCUS       DBMDYA010000330         3049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7192_length_3049_cov_1.621777, whole genome shotgun sequence.
ACCESSION   DBMDYA010000330 DBMDYA010000000
VERSION     DBMDYA010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_3049_cov_1.621777"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(162..1876)
                     /locus_tag="ACZPFK_08545"
                     /pseudo
     CDS             complement(162..1876)
                     /locus_tag="ACZPFK_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1886..2335)
                     /locus_tag="ACZPFK_08550"
     CDS             complement(1886..2335)
                     /locus_tag="ACZPFK_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1406400.1"
                     /translation="MMQIRTFLPQDRDAVLALVSDFYTSPACLHAIPVQNFADAFDEM
                     GADRGLLRGLALLEGERLCGYCQLAFTYSTEAGGPVVLLEELYLRPDCRGKGYGKQLL
                     DFLKKEYAGRAARLRLEVAPENPRARQLYEREGFEVLPYTQMILEDF"
     gene            complement(2407..>3049)
                     /locus_tag="ACZPFK_08555"
     CDS             complement(2407..>3049)
                     /locus_tag="ACZPFK_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1406401.1"
                     /translation="LTAALFAYFFFHRQVDAFCSHGLGALLKVVFFCGFAFFLCLVGL
                     MEVSALRAAPTGTERVVIVLGAGLRDDKPSDTLRRRLDAALEYYRGHPGCLLVLSGGQ
                     GPDEALPEAEAMARYLREAGVPQADLLLEDKSASTEENFSFSLRLLRRRGLDGDTPVL
                     FVTNRFHCYRAAGYARRVGFSRVSALPTGTSIFVWPAAVMREALAICAYWVKG"
BASE COUNT          585 a          937 c         1017 g          510 t
ORIGIN      
        1 gatcttgttg gcaaaggtgg ttttgccgga gccggagccc ccggcgatgc cgatgacgag
       61 cgcgtctctt ctttccatac gaaaacctcc cgtgatgggg ctctggggac gggggcccag
      121 agcctgtgcc ccctgcctgc cggcagcccg gtgatctgtt tttagtccat ggaaaattgt
      181 gtattgtaaa gggctgcata gaggccgccc tcttccagca gttgggcgtg cgagccggat
      241 tcggcgattc cgtcctcggt gaggaccaaa atgcggtcgg cgttgcgcac ggtggagagg
      301 cggtgggcca ccaccagggt ggtgcgcccg cgggagagct ggtcaaagct cttttggatg
      361 gcctgctcgg tgacggtgtc cagcgcgctg gtcgcctcgt ccaggatgag gatgggcggg
      421 tttttcaaaa agacccgcgc gatggcgatg cgctgcttct gcccgcccga cagcttgacc
      481 ccgcgctcgc cgatatcggt gtcatagccg tccggcatgg ccatgatggt gtcgtgcagc
      541 tccgcctcct tggccgcggc gacgatctcc tcctcggtgg cgtccagctt gccgtaggcg
      601 atgttctcgc ggatggtgcc ggtgaacagg aacacatcct gctgcacgat gccgatgttg
      661 cgccgcagct cccccgaggg gatgtcgcgc acgttgacgc cgccgatgcg caccgcgccg
      721 caggtgggct ggtaaaagcg ggggatgagg tggcagaatg tggtcttgcc gccgcccgag
      781 gggcccacca gggccaccgt ctcgccgcgg gggatgacgg tatcgatatt ctccagcacg
      841 gtcttgttct cgtcgtattg gaagctgacc ccctcaaagg cgatgtcggc ggaaaagctg
      901 tccggggtcg cggggcgcgc cggttccggc tcggggggcg tgtccatcac ctggcagaac
      961 cgctcaaagc cggacatgcc gtcctgaaac atctcgacga actggttgag ccgccggatg
     1021 gggtttgtga acatcgagat atagagcaga aaggcggcga agtcgccgat gtcgatgcgg
     1081 ccccaaaaga caaagagccc gctggcgacc agggccagca gcggcagcag gtcgatgccc
     1141 agctgcatgc cggagttgaa gaccgccatc acccggtaaa cgccctggcg ggcgctcaca
     1201 taggcgcggt tgcgcccctc aaacttttcg ccctcgtagt cggcgttgtc gaaagccttg
     1261 gccacccgga tgccggaaag gctgttttcg atctcggcgt tgatttgacc ggtctcctcc
     1321 cggctgcggg caaaggcttt cagcatcagc cgccgcaggc gaacggcgaa cagcaccagc
     1381 agcggcacaa agatgaagat gatcagggtc agcgccaggt cgatgcgggc cagcaggaca
     1441 aaggagagca caaaggtcac cagggagatg aacaggtcct ccggcccgtg gtgggccagc
     1501 tcggcgatct caaaggtgtc gttcaccacc cggctcatca gggcgccggt cttgttttcg
     1561 tcaaaaaagg agaaaggcag cgtttcaatg tggcgaaaca ggtcgcggcg catatccgcc
     1621 tggatcttca cccccaccat atgcccccag tagatggtga aataggtgag cagcgtcttg
     1681 atgagataga tgaccagcat cgccacggcc cagcccacga agagctgcag tttgcggccg
     1741 ggcacatagt cgttgatgac ggtgcgggtg agcatcgggt agaacaggtc gcaggcggcc
     1801 ataaaaaagc ggccagcaga tccagggtga acagcaccca gtggggcctg taataacggg
     1861 caaagcgtct gagcattttc acggcttaaa aatcctccag gatcatctgt gtatagggca
     1921 gcacctcaaa gccctcgcgc tcatagagct gccgcgcgcg cgggttttcg ggggcgacct
     1981 caaggcgcag gcgggcggcc ctgccggcgt attctttttt gagaaaatcc agcagctgct
     2041 tgccgtagcc cttgccgcgg cagtcggggc ggagatagag ctcttccaaa aggacgaccg
     2101 ggccgccggc ctcggtggag taggtgaaag cgagctggca atagccgcac agccgctcgc
     2161 cctccagcag ggcaaggccg cgcagcaggc cgcggtccgc ccccatctcg tcaaaggcgt
     2221 ctgcgaagtt ctgcaccggt atggcgtgca ggcaggcggg ggaggtgtaa aagtcggaca
     2281 ccagggccag caccgcgtcc cggtcctggg gcaaaaaggt tcggatctgc atcatgttct
     2341 cctttatctc tatggtttcc tatccgtgcg gccgcggttc agacgacagc gcaatcgcag
     2401 cgcgggtcat ccctttaccc agtaggcgca gatggcgagc gcctcccgca tgaccgccgc
     2461 cggccagacg aagatgctgg tgccggtggg cagggcgctc acgcgggaaa agcccacccg
     2521 gcgcgcatag cccgcggctc gatagcagtg aaagcggttg gtcacaaaca ataccggcgt
     2581 gtcgccgtct aatccgcgcc ggcgcagcag ccgcagcgaa aaagaaaaat tttcctcagt
     2641 ggaggcggat ttgtcctcca gcagcaggtc ggcctgcggc accccggcct cccgcaggta
     2701 gcgggccatg gcctcggcct cgggcagcgc ctcgtccggc ccctggccgc cgctgagcac
     2761 cagcaggcag ccggggtggc cgcggtagta ctccagcgcc gcgtccaggc ggcggcgcag
     2821 cgtgtcggag ggcttgtcgt cccgcagccc ggcgcccagc acgatcacca cccgctccgt
     2881 ccccgtgggg gccgcgcgca gggcgctcac ctccatcagg cccaccaggc acagaaaaaa
     2941 ggcaaagccg caaaaaaaca cgaccttgag cagcgcgccc aggccgtggc tgcagaacgc
     3001 atccacctgg cggtgaaaaa agaagtaggc gaaaagggcg gcggtgagg
//
LOCUS       DBMDYA010000331         3047 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7201_length_3047_cov_1.646640, whole genome shotgun sequence.
ACCESSION   DBMDYA010000331 DBMDYA010000000
VERSION     DBMDYA010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_3047_cov_1.646640"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            835..1515
                     /locus_tag="ACZPFK_08560"
     CDS             835..1515
                     /locus_tag="ACZPFK_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1406402.1"
                     /translation="MPYKLLVADDEAPLRALLQDAFSMLGYTVLCAANGREAVALAGQ
                     GPDLILLDVNMPDLDGLEVCRRIREHVACPILFLTARVEDADKIEGFSAGGDDYILKP
                     FSLDELEARVAAHLRRQQRAASSPLARVRFEDDLAVDYAAHAVTCGGRPVPLAKKEYE
                     ILELLSRHAGQVFDRERIYEALWGFDAEGSSAVVAEHIKRLRAKLAAAGAREHIETKW
                     GMGYRWIR"
     gene            1512..2831
                     /locus_tag="ACZPFK_08565"
     CDS             1512..2831
                     /locus_tag="ACZPFK_08565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1406403.1"
                     /translation="MKRAFSLKWSFAQYLIVCLVVAGLLCLLTALPCEILRNALGEKY
                     RDYYESDAFLWSYVVDENGNTVGRTRIGITAPDGSAVTLEDYMEPRDRMIYTLLQYYP
                     LLMPLYFAASVAVTSVLFYRRRLKRPLEALDAAAGRIAAGELEFAVEVPGRDELHRLG
                     ESFDTMRAALAENSRELWRTLEDSRRMQAAFAHDLRTPLTVLRGYNEFLLKYQGRLSE
                     EKVRGTLETMHASIERLERYTQRMGAAQKLERLPLQKTPTDLEELAAALARDGAILCG
                     QACAFEMKTEGLSGTAALDAALVREVFENLTANAARYAETRVEVECVRKDGCLAVTVR
                     DDGPGFSPSALAHAQEAFWREETVEAARGEDHFGLGLHLCAALCQKHGGALALGPAPG
                     RGAEATATFALTPQAAAHAPAPPAGTAAQPGAGRRKKSREMREQIQE"
BASE COUNT          550 a          913 c         1043 g          541 t
ORIGIN      
        1 accggcaaaa aaagtccctg ctgcgcaggc ttttcggcgg ggataaataa aaaaccgggc
       61 gctcggcgcc gcaggcatca aggtcacaag gccccgggca ctctgcgccg gggccttatg
      121 aaaaatagcc gtaaggctat ttttgggcca tccggccagt ccgctggccg gggccataga
      181 aataagcttt ttattgcgaa gcaataaaaa tagccgcttt gcggttattt tttggccatg
      241 tggcccgtcc ccgagccacg gccattaaag taagttgttt tttgtgacca tccatcccgt
      301 cctccgccca tgcctataaa gagccgctca gcggctgttt gtttcagcca tctgtttcga
      361 gcgccatgcg gcagcggcaa gcgggtccgt caagagtgct gtcttttatc tcccattccg
      421 cttttccctg cgcggcttcg gcggcttggc tcctcccgtc cggcgctgct gcggttttct
      481 ctccccgcct gaaaagggcg cgcgacgctt ggctcccttt tcagggacgg catacgcgca
      541 gcgccgggag aaagaagccg acgccgggcc ccgctctcct gtgtcgggcc gtcaagcgct
      601 cgggtcctgt aataggcggc tgcgctgctg tcgctttgag cgtcgcggcc ggggcgaagt
      661 taccgagttg ataaaaagtt tatttttgtg ttataagctg acggcggtgc cgcgggcgcc
      721 cgcctgcttg cctttgtagc ggcccttcag gggcaaagcc gccggcgccg aaaaacaggg
      781 gatgagagag cgccgccccg cctgtagggg cggcccccac caaggagagg accaatgcca
      841 tacaagttgc tggtggcgga cgatgaagcg ccgctgcgcg ccctgctgca ggatgctttt
      901 tccatgctgg gctacacggt gctctgcgcg gccaacggcc gagaggccgt tgcgctggca
      961 gggcaggggc cggacctgat tttgctggac gtcaacatgc ccgatctgga tgggctggag
     1021 gtctgccgcc gcatccggga gcatgtggcc tgcccgatcc tgttcctcac cgcccgggtg
     1081 gaggatgcgg acaagatcga gggcttctcc gccgggggcg acgattacat cctgaagccc
     1141 ttttcactgg acgagctgga ggccagagtg gccgcccatc tgcgccgcca gcagcgggcg
     1201 gcgtcaagcc cgctggcgcg ggtgcgcttt gaggacgatc tcgccgtcga ctacgccgcc
     1261 catgccgtca cctgcggcgg caggccggtc ccgctggcga aaaaagaata tgagatactg
     1321 gagctgctct cccgccacgc gggccaggtc tttgaccggg agcgcatcta tgaggccctg
     1381 tggggcttcg acgcggaggg ctcctcggcg gtggtggccg agcacatcaa acggctgcgg
     1441 gcaaagctgg cggccgcggg cgcgcgggag catatcgaga cgaaatgggg gatggggtac
     1501 cgttggataa gatgaagcgg gctttttccc tcaaatggag ctttgcccag tatctgatcg
     1561 tctgcctggt ggtggcgggg ctgctgtgcc tcctcaccgc cctgccctgc gagatcctgc
     1621 gcaacgcact aggggaaaaa tatcgcgatt actacgagag cgacgccttt ttgtggtcct
     1681 atgtggtgga cgaaaacggc aatacggtgg gcaggacccg catcgggatc accgcgccgg
     1741 acgggtcggc ggtgacgctg gaggattaca tggagccccg ggaccggatg atatatacgc
     1801 tgcttcagta ttatccgctg ctgatgcctc tctattttgc cgccagcgtg gcggtgacca
     1861 gcgttctgtt ctaccggcgc aggctgaaac ggcccctgga ggccctggac gcggcagcgg
     1921 ggcgcatcgc ggcgggcgag ctggaatttg cggtggaggt gcccgggcgc gatgagctgc
     1981 accgcctggg ggaatccttt gacacaatgc gcgcagcgct ggcggagaac agccgcgagc
     2041 tgtggcgcac gctggaggac agccgcagga tgcaggcggc gtttgcccac gatctgcgca
     2101 cgcccttgac cgtgctgcgg ggctataacg agtttttgct gaaataccag ggcaggctga
     2161 gcgaagaaaa ggtgcgcggc accctggaga ccatgcacgc cagcatcgag cggctggaac
     2221 gctataccca gcgaatgggc gcggcccaaa agctggagcg cctgcctctg caaaagacgc
     2281 cgaccgacct tgaggagctg gccgccgcgc tggcccggga cggggcgatc ctctgcgggc
     2341 aagcctgcgc ctttgaaatg aaaacggagg gcctgtcggg cactgcggcg ctggacgcgg
     2401 cgctggtgcg cgaggtgttt gaaaatctga cggccaatgc cgcccgctat gccgagaccc
     2461 gggtggaggt ggaatgtgtg cgcaaagacg gctgcctggc ggtcacggtg cgggacgacg
     2521 ggccggggtt ttcaccctct gcgctggccc atgcacagga ggcgttctgg cgcgaggaga
     2581 ccgtggaagc ggcacgggga gaggaccact tcggcctggg gctgcacctg tgcgcggcgc
     2641 tctgtcaaaa gcacgggggc gcgcttgccc tcggccccgc gcccggcagg ggcgccgagg
     2701 ctacggccac ctttgccctg acgccgcaag cggcggcgca cgcccccgcc ccaccggcgg
     2761 ggacagcggc gcagccgggg gcggggagaa gaaaaaagag ccgcgaaatg cgggagcaga
     2821 tacaagaata gataaaaagt ttaagatcgc ctgttacact cttttcaacc caaagcgagg
     2881 agagtgtatt ttttatgccg gagaccatga tcgaaatcga gggcctgtgc cagagctacg
     2941 gcaggaagct ggcggtggac cacctgacgc tgcgcatccc caggggcatg ttcggcctgc
     3001 tgggaccgaa cggcgcgggc aagaccaccc tcatgcgcac catcgcc
//
LOCUS       DBMDYA010000332         3043 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7214_length_3043_cov_1.245411, whole genome shotgun sequence.
ACCESSION   DBMDYA010000332 DBMDYA010000000
VERSION     DBMDYA010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7214_length_3043_cov_1.245411"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="ACZPFK_08570"
     CDS             <1..782
                     /locus_tag="ACZPFK_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HIR1406404.1"
                     /translation="IDEALAAPRAPMAVPQNRFPLLPMPRLAEVALDKELDKGEYNDR
                     LETAQKRLRELHNRLYRKKVPLILAYEGWDAAGKGGNIKRVAAALDPRGFEVLPIASP
                     EPHELAHHYLWRFWTRLPKDGHVAIFDRSWYGRVMVERIEGFCSQNDWQRAYNEINEF
                     ESELAAWGAVILKFWIHIDPETQLARFHERQANPEKQWKITEEDWRNREKWPQYEEAV
                     DEMLQKTSTTYAPWHIIESRDKKYARVKALELIVAALEEVL"
     gene            782..1288
                     /locus_tag="ACZPFK_08575"
     CDS             782..1288
                     /locus_tag="ACZPFK_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1406405.1"
                     /translation="MEIERKWEIGGFPGGLPQTDAAEMRQGYLVTDPVVRIRAEQRAG
                     GGSYILCIKGRGTLAREEIETPISEAVFERVAALIGAPLVRKRYRAYRLPDGHTLEVS
                     LVDEGEETAFYYAEVEFATLEEARAFEPPAFLGRELTEAPGASMSAYWRKRAAAWQKK
                     HPEGEARL"
     gene            1285..>3043
                     /locus_tag="ACZPFK_08580"
     CDS             1285..>3043
                     /locus_tag="ACZPFK_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406406.1"
                     /translation="MKRQTGTSAKGGSPVEPRAKSPALGARRWAALLIFGLFGQLAWT
                     IENMYFNVFVYNTISTDPGVIADMVAWSAVTATATTLLMGALSDKLGRRKGFIVGGYI
                     VWGLSVMAFSLISVQNAAKLFPAADAARMAALLVVVMDCVMTFFGSTANDAAFNAWVT
                     DVTDEHNRGRVEAVLAILPLVAMLVIFGGFDWMTAAGRWSEFFLIFGGLVTLGGFLGL
                     LFVQDAPGLRRAESSYFKNIVYGFRLSVVRQNPRLYLAFLGLAVFGASTQVFMPYLII
                     YIQRYLGIDNYALVLGVVLIGASAVSVASGRFIDRLGKLRFVLPAAAVEVAGLLAMVF
                     ARGAAAVIGAGFVLLSGNMLVTAALNGLARDYTPRDKAGHFQGIRMLFAVLLPMVTGP
                     YLGAAVIRGSGAVYEELGVMKQVPTPAIFLASAAVLVFVAVPVLLLKGRQRRSEPLKE
                     LLTPWGERLDPDAPLPEYPRPQFARGEGSWRSLNGRWRYAIRPDGQPMGGADGQIVVP
                     FSPESPLSGVRRQLQPGETLWYEREFRVSGLPGKGKLLLHFGAVDQRCTVWVNGAEAG
                     RHGDGYLPVTLDITDLVREG"
BASE COUNT          457 a          968 c         1128 g          490 t
ORIGIN      
        1 ccatcgacga ggcgctggcc gccccgcggg cccccatggc ggtgccgcag aaccgctttc
       61 cgctgctgcc catgccgcgg ctggccgagg tggcgctgga caaggagctg gacaagggcg
      121 agtacaacga ccggctggag acggcccaga agcggctgcg cgagctgcac aaccgccttt
      181 accgcaaaaa ggtgccgctg attttggcct acgagggctg ggacgccgcc ggcaagggcg
      241 gcaacatcaa gcgggtggcc gcggcgctgg acccgcgcgg ctttgaggtg ctgcccatcg
      301 ccagccccga gccccacgag ctggcccacc actatctgtg gcggttctgg acccggctgc
      361 ccaaggacgg ccacgtggcc atctttgacc gcagctggta cggccgggtg atggtggagc
      421 gcatcgaggg cttttgcagc cagaacgact ggcagcgcgc ctacaacgag atcaacgagt
      481 ttgagtcgga gctcgccgcc tggggcgcgg ttattttgaa gttctggatc cacatcgacc
      541 cggagaccca gctggcccgc ttccacgagc ggcaggcaaa ccccgaaaag cagtggaaga
      601 tcaccgagga ggactggcgc aaccgggaga agtggcccca gtacgaggag gcggtggacg
      661 agatgctgca aaagaccagc accacctacg ccccctggca catcatcgag tcgcgggaca
      721 aaaaatacgc ccgcgtcaag gcgctggagc tgatcgtcgc cgcgctggaa gaggtgctgt
      781 gatggagatt gagcgcaagt gggagatcgg cggcttcccc ggcgggctgc cgcagaccga
      841 cgccgccgag atgcggcagg gctatctcgt caccgacccg gtggtgcgca tccgcgccga
      901 gcagcgcgcc ggcggcggca gctatatcct gtgcatcaag ggccgcggca cgctggcccg
      961 cgaggagatc gagaccccca tcagcgaagc ggtttttgag cgggtggcgg ccctgatcgg
     1021 cgcgccgctg gtgcgcaagc gctaccgcgc ctaccgcctg ccggacgggc acacgctgga
     1081 ggtgagcctg gtggacgagg gggaggagac cgccttttac tacgccgagg tggagttcgc
     1141 cacgctggag gaggcgcgcg cttttgaacc gcccgccttt ttgggccgcg agctgaccga
     1201 ggcgccgggg gcgtcgatga gcgcctactg gcgcaaaagg gccgcggcct ggcagaaaaa
     1261 acatcccgag ggggaggcgc gtttatgaaa cggcagacgg gaacgtccgc aaagggaggc
     1321 tcgcccgtgg agccgcgggc aaaatcgccc gcgctggggg cgcgccgctg ggcggcgctg
     1381 ctgatcttcg gcctgttcgg gcagctggcc tggacgatcg agaacatgta cttcaacgtc
     1441 ttcgtctaca acaccatctc caccgacccg ggggtcatcg ccgacatggt ggcctggagc
     1501 gccgtcaccg cgacggccac cacgctgctg atgggcgcgc tgtccgacaa gctgggcagg
     1561 cgcaagggct tcatcgtggg gggctatatc gtctggggcc tgtcggtgat ggcgttcagc
     1621 ctgatctcgg tgcagaacgc ggcgaagctg ttcccggcgg cggacgcggc ccgcatggcg
     1681 gcgctgctgg tggtggtcat ggactgcgtg atgacctttt tcggctccac ggccaacgac
     1741 gcggccttta acgcctgggt caccgacgtc accgacgagc acaaccgggg cagggtggag
     1801 gcggtgctgg ccatcctgcc gctggtggcg atgctggtga tcttcggcgg ctttgactgg
     1861 atgaccgcgg ccgggcgctg gagcgagttc tttctcatct tcggcggcct ggtgacgctg
     1921 gggggctttt tgggcctgct cttcgtgcag gacgcgccgg ggctgcggcg ggccgagagc
     1981 agctatttca agaacatcgt ctacggcttc cgcctctcgg tggtgcggca gaacccgcgg
     2041 ctgtatctcg cctttctggg gctggcggtc ttcggggcct ccacccaggt cttcatgccc
     2101 tatctcatca tctacatcca gcggtatctg ggcatcgaca actacgcgct ggtgctgggg
     2161 gtggtgctta tcggcgcctc ggcggtgagc gtggccagcg gccgcttcat cgaccgcttg
     2221 ggcaagctgc gctttgtgct gccggcggcg gcggtggagg tcgcgggcct gctggcgatg
     2281 gtctttgccc gcggcgcggc ggcggtgatc ggggccgggt tcgtgctgct gtccggcaac
     2341 atgctggtga cggcggccct gaacggcctg gcgcgggact acaccccccg ggacaaggcg
     2401 gggcattttc agggcatccg catgctgttt gcggtgctgc tgcccatggt caccgggccc
     2461 tatctcggcg cggcggtcat ccgcggctcg ggcgcggtct atgaggagct gggcgtgatg
     2521 aagcaggtgc ccacgccggc catctttttg gccagcgccg cggtgctggt gttcgtggcc
     2581 gtgccggtgc tgctgctcaa gggccgccag cgccgcagcg agccgctgaa agagctgctc
     2641 accccctggg gcgagcggct ggacccggac gcgcccctgc ccgagtaccc gcgcccgcag
     2701 ttcgcccgcg gcgagggcag ctggcgcagc ctcaacggcc gctggcgcta tgccatccgg
     2761 ccggacgggc agcccatggg cggggcggac gggcagatcg tggtgccctt ttcgcccgag
     2821 agcccgctgt ccggcgtgcg gcggcagctg cagcccggcg agacgctgtg gtacgagcgg
     2881 gagttccggg tgtccggcct gccgggcaag gggaagctgc tgctgcactt cggcgcggtg
     2941 gaccagcgct gcacggtgtg ggtgaacggc gccgaggcgg gccgccacgg cgacggctat
     3001 ctgccggtca cgctggacat caccgacctg gtgcgggagg ggg
//
LOCUS       DBMDYA010000333         3028 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7261_length_3028_cov_1.448241, whole genome shotgun sequence.
ACCESSION   DBMDYA010000333 DBMDYA010000000
VERSION     DBMDYA010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7261_length_3028_cov_1.448241"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(49..432)
                     /locus_tag="ACZPFK_08585"
     CDS             complement(49..432)
                     /locus_tag="ACZPFK_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1406407.1"
                     /translation="MQIHQSAEDYLETILILKERKGMVRSIDIANELQFTKASVSVAM
                     KKLRENGYAEMDPEGFITLTKAGMEVASRIYDRHKLLTEFFVRLGVSEKTAAEDACRI
                     EHDISEETFEKLLAHARKNGGGKAE"
     gene            708..920
                     /locus_tag="ACZPFK_08590"
     CDS             708..920
                     /locus_tag="ACZPFK_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1406408.1"
                     /translation="MMPLTMARPGDTVTIRKITGRDEVRQHLAELGFVVDGTVTVVSE
                     IAGNLILQVKESRVALDRTLANRVLF"
     gene            959..1180
                     /locus_tag="ACZPFK_08595"
     CDS             959..1180
                     /locus_tag="ACZPFK_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1406409.1"
                     /translation="MKTLKDVKVGETVTVRRLHGEGPVKRRIMDMGITKGVDLHLRKV
                     APLGDPMELNVRGYELSVRKADAEMIEVE"
     gene            1271..>3028
                     /gene="feoB"
                     /locus_tag="ACZPFK_08600"
     CDS             1271..>3028
                     /gene="feoB"
                     /locus_tag="ACZPFK_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1406410.1"
                     /translation="MSITIALAGNPNCGKTTMFNDLTGSNQYVGNWPGVTVEKKEGRL
                     KGHKDVVIQDLPGIYSLSPYTLEEVVARGYLVREKPDAIINIVDGTNVERNLYLTTQL
                     IELGIPVVIAMNMIDIVRRNGDRIDLDKLGAELGCTVIETSALKGEGSMAAAEKAIEA
                     AMAHRVGEHPHVFTGSVEHALAHIEESIGDKVEAQNLRWYAIKLFERDEKVAAELNLD
                     AALAGHLESHIQDCEKELDDDAESIITNQRYAYISKVVDHCVKKKAARHSLSASDKID
                     RVVTNRVLALPIFAVAMFLVYWLAMGPLGTFLTDWTNDVFAAEWLQAGSRALLEGWGV
                     AEWLIGLVSDGIIGGVGAVLGFVPQMLVLFLLLAVLEDVGYMARVAFIMDRIFRKFGL
                     SGKSFIPMLIGSGCGVPGVMASRTIENDRDRKMTIMTTCFVPCGAKMPIIGLFAGALF
                     GGSGLVATSAYFIGVGAIILSGVMLKKTKPFAGEPAPFVMELPAYHVPSGKNVFHATW
                     ERGWSFIKRAGTVILASSVILWFLQGFGFVDGVFGMVEDNNSSLLAAVGSAAAFLFAP
                     LGFGDWQSTVATVTGLIAKE"
BASE COUNT          577 a          926 c          955 g          570 t
ORIGIN      
        1 cgaggacggg ccacatggcc gaaaaaacag ccgctatgcg gctgtttttt attccgcttt
       61 ccctcccccg tttttgcggg cgtgggccag cagcttttca aacgtctcct cgctgatatc
      121 gtgctcgatg cggcaggcat cctccgccgc cgtcttttcg ctcacgccca gccgcacaaa
      181 aaactcggtc agcagcttgt gccggtcgta gatgcggctg gcgacctcca tgcccgcctt
      241 ggtcagggtg ataaacccct ccggatccat ctcggcatag ccgttttccc gcagcttttt
      301 catggccacg ctgacgctgg ccttggtaaa ctgcagctcg ttggcaatgt cgatagagcg
      361 caccatcccc ttgcgctctt tcaagatgag gatcgtctca agataatctt ctgcggactg
      421 atggatctgc aagtgatgac ctccttacgg aaaacgccct gcgggcccca caaaagcccc
      481 ccgcgcgccg tctgcgcctc gcgccgggga aaggccgccc cttttctgag ctatgctgta
      541 agggtatcat actcaggcgc ttttagcaag cgcgccaaac gacaaacgat tttcttccca
      601 gctcttgaca gcggcggtta gttgtggtaa actttgcatc gtccaagaaa gttagttatt
      661 tctaaccaag ctctgcggcg cccgccgcag cgagaggagg agaggccgtg atgcctttga
      721 cgatggcgag gccgggcgac accgtgacca tccgcaagat caccggcagg gacgaggtgc
      781 gccagcacct ggccgagctg ggctttgtgg tggacggcac ggtgaccgtg gtgagcgaga
      841 tcgcgggcaa cctgattttg caggtgaaag agagccgggt ggccctggac cgcacgctgg
      901 caaaccgggt gctgttttaa aaaccaaggc cgatgtactg aaaaggagga atagaggcat
      961 gaagacactg aaagacgtca aggtcggcga gaccgtgacg gtgcggcggc tgcacggcga
     1021 ggggccggtg aagcgccgca tcatggacat gggcatcacc aagggggtgg acctgcacct
     1081 gcgcaaggtg gcgccgctgg gcgacccgat ggaactgaac gtgcgcggtt atgagctgag
     1141 cgtgcgcaaa gccgacgcgg agatgatcga ggtcgagtga ccccggggct tgcgggcctt
     1201 tattttttcg cctcgaggtt agctaaagct aacttcatgc gggcggatca ccaaggagga
     1261 ggcagaatac atgagtatca ccattgcgct tgccggcaac cccaactgcg gcaagaccac
     1321 catgttcaac gacctgacgg ggtccaacca gtatgtgggc aactggcccg gcgtcacggt
     1381 ggaaaagaaa gagggccggc tgaagggcca caaggacgtg gtcatccagg acctgccggg
     1441 catttattcc ctctcgccct acacgctgga ggaggtcgtc gcccgcggct atctggtgcg
     1501 ggaaaaaccg gacgccatca tcaacatcgt ggacggcacc aatgtggagc gcaacctcta
     1561 cctgaccacc cagctcatcg agctgggcat cccggtggtc atcgccatga acatgatcga
     1621 cattgtgcgc aggaacggcg acaggatcga ccttgacaag ctgggggccg agctgggctg
     1681 caccgtgatc gagaccagcg ccctcaaggg cgagggcagc atggcggcgg cggaaaaagc
     1741 catcgaggcc gccatggccc acagggtggg cgagcacccc cacgtcttca ccggcagcgt
     1801 ggagcacgcc ctcgcccata ttgaggagtc gatcggggac aaggtggagg cacagaacct
     1861 gcgctggtac gccatcaagc tgttcgagcg ggatgaaaag gtcgccgccg agctgaacct
     1921 ggacgcggcc ctcgccggcc atctggaatc ccatatccag gactgtgaaa aagagctgga
     1981 cgacgacgcc gagagcatca tcaccaacca gcgctacgcc tatatcagca aggtggtgga
     2041 tcactgcgtg aagaaaaagg cggccaggca cagcctgtcc gcctcggaca agatcgaccg
     2101 tgtcgtcacc aaccgcgtgc tggcgctgcc gatcttcgcg gtcgccatgt ttctcgtcta
     2161 ctggctggcc atggggccgc tgggcacctt tctcaccgac tggaccaacg acgtgttcgc
     2221 cgcagaatgg ctgcaggcgg gctcgcgggc gctgctggag ggctggggcg tggccgaatg
     2281 gctgattggc ctggtctcgg acggcatcat cggcggcgtg ggcgccgtgc tgggctttgt
     2341 gccgcagatg ctggtgctgt ttttactgct cgccgtgctg gaggacgtcg gctatatggc
     2401 ccgcgtcgcc tttatcatgg atcgcatctt ccgcaagttc ggcctctcgg gcaagagctt
     2461 catccccatg ctcatcggct cgggctgcgg cgtgccgggg gtgatggcct cccgcaccat
     2521 tgagaacgac cgcgaccgca agatgaccat catgaccacc tgctttgtgc cctgcggcgc
     2581 caagatgccc atcatcggcc tgttcgccgg ggccctgttt ggcggcagcg gtctggtggc
     2641 caccagcgcc tacttcatcg gcgtgggcgc catcatcctc agcggcgtca tgctgaaaaa
     2701 gacaaagccc tttgcgggcg agcccgcccc ctttgtcatg gagctgcccg cctaccacgt
     2761 gcccagcggc aagaacgtgt tccacgccac ctgggagcgg ggctggagct ttatcaagcg
     2821 cgccggcacc gtcatcctcg cctcctccgt catcctctgg tttttgcagg gcttcggctt
     2881 tgtggacggc gtcttcggca tggtggagga caacaactcc agcctgctgg ccgctgtggg
     2941 cagcgccgcc gcttttctct ttgcgcccct gggctttggc gactggcagt ccaccgtggc
     3001 gacggtcacc ggcctgatcg ccaaggag
//
LOCUS       DBMDYA010000334         3021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7280_length_3021_cov_1.449315, whole genome shotgun sequence.
ACCESSION   DBMDYA010000334 DBMDYA010000000
VERSION     DBMDYA010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7280_length_3021_cov_1.449315"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="ACZPFK_08605"
     CDS             <1..511
                     /locus_tag="ACZPFK_08605"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1406411.1"
                     /translation="FIANIPTEEVFTAPHRLRVNGVVKGTKPYVYNGNLIEGFSVTFK
                     DGEVVEHSAQSGEALLGQLLDTDEGSRRIGEIALVPASSPINRRGILFYNTLFDENAA
                     CHIAFGDGYPGTVAGGTGLTQEELLARGVNHSVVHEDVMIGAEDTRIVGVAGDGRRVE
                     IFENGVWAL"
     gene            551..1684
                     /locus_tag="ACZPFK_08610"
     CDS             551..1684
                     /locus_tag="ACZPFK_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HIR1406412.1"
                     /translation="MRISPEIGMNIVTEMKKIIHEDLTFMDTSGQIIACTDEKRIGDF
                     HAASRKMLDEGLKELTVPDDDTYAGTRSGINLALEIEGQIVGVVGITGKESEVSRYGQ
                     VVKKMTEILLMENDLKEQRLRERRARNRFLQEWLMADELRMNPALTNEGLAQGVDMTV
                     PRRIAVLEPATDSILSQEEVELAEHRIRSILREDARNLSGSNGGSFVVLMTERGDAQA
                     VRLIGQIQQAVQRECGRRLLAGIDSQTVSGGQLKAGYFRAKKALNGALAKGGDGIALY
                     SSILMEIFINDVPESSKKEFVRRVFRDCSEEKIGEYIRILRVLYGCNGSITRAAEQLF
                     LHKNTVQYKLNRISERTGYDPRKLGEAPYFYLAMTFYEDMHRD"
     gene            1966..>3021
                     /gene="larA"
                     /locus_tag="ACZPFK_08615"
     CDS             1966..>3021
                     /gene="larA"
                     /locus_tag="ACZPFK_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR1406413.1"
                     /translation="MKIELPYGKTKLSAELPSERVAAMLHSRLADLEPCAPAETLVEQ
                     ALANPIGSPPLWELARGKKRVVLLASDHTRPVPSRVIVPAMLREVRRGNPQAEITILV
                     ATGCHRGSTAAELEQKFGPELCASERILVHDCARDPMVDLGTLPSGAPLEVNQVAAQA
                     DLLIAEGFIEPHFFAGFSGGRKSVLPGVCSSRTVYANHCAALIGDPHARSGCLEGNPV
                     HRDMVEGARRAGLRFIVNVACNGRKQVVAAFAGELEKAHLAGVEFLHGACRLEVEQPA
                     GIVITGNGGYPLDQNIYQAVKGMSTADIVCREGGVIIMASACADGHGGESFYRTFAGG
                     ETAGEILERIERTPMEET"
BASE COUNT          648 a          807 c         1025 g          541 t
ORIGIN      
        1 attcatcgca aatatcccca ccgaggaagt gttcaccgcg ccgcaccgtc tgcgcgtaaa
       61 cggcgttgta aagggcacca agccctatgt gtacaacggc aacctcattg agggcttttc
      121 cgtcaccttc aaggacggcg aagtggtgga gcattcggcc caaagcggcg aggcgctgct
      181 gggccagctg ctggacaccg atgaaggctc ccgccgcatc ggcgagatcg cgctggtgcc
      241 ggcgtccagc cccatcaacc ggcggggcat tttgttctac aatacgctgt tcgacgagaa
      301 cgcggcctgt cacatcgcct ttggcgacgg ctatccgggc acggtggcgg gcggcaccgg
      361 cctgacgcag gaggaactgc tggcccgggg cgtcaaccac agcgtggtgc acgaggacgt
      421 gatgatcggc gctgaggaca cccgcatcgt cggcgtcgcc ggggacgggc gccgggtgga
      481 aatttttgaa aacggggtct gggcgctctg agcctccgcc ggaagcaagc ccacaaaagg
      541 agggatcagc ttgcgtatct cacctgaaat agggatgaat attgtcaccg agatgaaaaa
      601 gatcattcat gaggatctga cttttatgga taccagcggc cagatcatcg cctgcacaga
      661 cgagaagcgg atcggggact ttcacgcggc cagccgcaag atgctggacg agggcctgaa
      721 agaactgacc gtgccggacg acgacaccta tgcgggcacc cgctccggta tcaatctggc
      781 gctggagatt gaggggcaga tcgtgggtgt ggttggcatc accggcaaag agtcggaggt
      841 gagccgatat gggcaggtcg tcaaaaagat gaccgagatt ctgctgatgg aaaacgacct
      901 gaaagaacaa cggctgcggg agcgcagggc tagaaaccgc tttttgcagg agtggctgat
      961 ggccgatgag ctgcgcatga acccggccct gaccaacgag gggctggcgc agggcgtgga
     1021 tatgacggtg ccgcggcgta tcgcggtgct ggagccggcg accgattcca tcctgagcca
     1081 ggaggaggtc gaactggccg agcaccgcat ccgcagcatc ctgcgggagg acgcccgcaa
     1141 cctgagcggc agcaacggag gcagctttgt ggtgctgatg accgagcgcg gcgacgccca
     1201 ggccgtgagg ctgatcgggc agatccaaca ggcggtgcag cgggagtgcg gccgccgtct
     1261 gttggcgggc atcgacagcc agacggtgag cggtgggcag ctgaaagccg gctattttcg
     1321 ggcgaaaaag gctttaaacg gcgccctggc caagggcggc gatggcatcg cgctgtacag
     1381 cagtattttg atggaaattt ttatcaacga cgtgccggag agcagcaaaa aggaatttgt
     1441 gcgccgtgtg ttccgggact gctcggagga gaagatcggc gagtacatcc gtatcctgcg
     1501 agtgctgtac ggctgtaacg gttccattac cagggcggcg gagcagcttt ttttgcataa
     1561 gaacaccgtg cagtataagc tgaaccgcat cagcgagcgc acaggctatg acccgcgcaa
     1621 gctgggggag gccccttatt tttatctggc gatgacgttt tatgaggata tgcaccggga
     1681 ttgagcgggg gattttttca aaatgataag agggccggga gcgtgggccg catgctcccg
     1741 gccctctttg cgcgcagatc aggtgacgga aaaaagagcg gagggcgggg aggattttgt
     1801 gagagatggg taaaaaaagg gacaaaactt tttttagtat ggtatataag accataaaat
     1861 aaacaaaaaa ctggttttta taacatatcc ggcgctattg aagatgtgtt atatttgtga
     1921 tagcaaagag acacggccgg tcttttggga ggaggctttc atgaattgaa gatcgaattg
     1981 ccctatggaa aaacgaaact ctcggcggag ctgccaagcg agcgggtggc cgcgatgttg
     2041 cacagccggc ttgccgatct ggagccctgc gccccggcgg agacattggt ggaacaggcg
     2101 ctggccaacc ccatcggcag ccccccgctc tgggagctgg cgcgggggaa aaagagggtg
     2161 gtcctgcttg ccagcgacca cacccgacca gtgcccagcc gggtgatcgt gcccgccatg
     2221 ctgcgggagg tacggcgggg aaacccgcag gcggagatca ccatcctggt agccaccggt
     2281 tgccatcggg gcagcaccgc agcggaactg gagcaaaaat ttggtcccga gctctgcgcc
     2341 tctgaacgca tcctggtaca cgactgtgcc cgggacccca tggtggacct gggcaccctg
     2401 ccctccggcg cgcctctgga agtgaaccag gtggcggccc aagctgattt gctgatcgcg
     2461 gagggattta tcgagccgca tttttttgcc ggcttctccg gcggcagaaa aagcgttctg
     2521 ccgggggtgt gctcgtcccg caccgtctac gcgaaccact gcgcggcgct gatcggagac
     2581 cctcacgccc gcagcgggtg tctggagggc aaccccgtcc accgggacat ggtggaaggg
     2641 gcgcgccggg cgggcctgcg ctttattgtc aacgtggcct gtaacgggcg caaacaggtg
     2701 gtggccgcct ttgccggcga gctggagaaa gcccatttgg ctggggtcga atttttgcac
     2761 ggcgcctgcc gcctggaggt ggaacaaccc gcggggatcg taatcaccgg caacggcggg
     2821 tatccgctgg atcagaacat ctatcaggcg gtcaagggca tgtccaccgc cgacatcgtt
     2881 tgccgcgagg gcggggtcat catcatggcg tccgcctgtg cggacgggca tggaggagag
     2941 agcttttaca ggacctttgc gggaggagaa actgcggggg agatcttgga gcggattgaa
     3001 cgcacaccga tggaagagac a
//
LOCUS       DBMDYA010000335         3017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7289_length_3017_cov_1.905007, whole genome shotgun sequence.
ACCESSION   DBMDYA010000335 DBMDYA010000000
VERSION     DBMDYA010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7289_length_3017_cov_1.905007"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="ACZPFK_08620"
     CDS             <1..948
                     /locus_tag="ACZPFK_08620"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1406414.1"
                     /translation="RIIRSLKEKGIAVIFITHRLDEIFAICDRMTVLRDGCYIGTRDV
                     KDVTKSEMIAMMIGRELKQQFPERHAEIGEECLRVEHMSDGGRHVKDVSFSVRRGEVL
                     GFAGLVGAGRTETMRLIFGADHKARGKVFIDGREVSIASPRQAVAHRIGFVTENRKEE
                     GLFLSLPVRNNIVAVAIDKIKSFHILNFNREKEISDKYVRDLQIATPSINQRVVFLSG
                     GNQQKVVLAKWLLSDSQVMILDEPTRGIDVGAKREIYEIINQLAAQGKAIIVVSSEME
                     EIMGISDRILVMHEGEISGEVKKEDFSQSTITEYAVGGA"
     gene            950..1924
                     /locus_tag="ACZPFK_08625"
     CDS             950..1924
                     /locus_tag="ACZPFK_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406415.1"
                     /translation="MGDKNAIKEKTSFGQKLNKFTDAMGVWLVVLLFFIILSLATDNF
                     LTYANLTNVVRQICVNALVALGASYVILGGEIDLSTSNMCAFAGCGAALLMRAGVNMW
                     LAMLIMLVIGALIGCLTGVIVNYLKIPSFIASLGMQYVILGTTLLLTNSEPISGLPEG
                     FMTFGRGYVFDVIPVPVIILAIMFAVGGFVFKYTSFGRSVISVGENITAAKLSGINVS
                     RTKVAIFSIGGFSAALAGIVLASRLSSGQPMAGTDIGLQAIAAVYVGGTSKGSALNVL
                     AGSLALGLVNNGLNLLEVNAYWQKVALGVIIIFAVLMDQVRASKTAKK"
     gene            1948..2784
                     /locus_tag="ACZPFK_08630"
     CDS             1948..2784
                     /locus_tag="ACZPFK_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406416.1"
                     /translation="MKIAYTAWTWLQDEYNAWAPMSDKPKRDFEASLRDLHACGYRYL
                     EDFNIVADIFDGCPEEFAALTRKYEIEFVNLYHYLTADFEYDRKKTEQCIAFMKAQGT
                     TQMNLEPPRKAPGQVIGEKELAEVAQHCDILGGMCRDAGISLALHPHWGTYVEREDQI
                     DYFLAHTRRDCVGLCLDTAHTVLCGMDPEKLFAKYLAQGVLKYVHFKDIDPDETAHPE
                     YPPRRFRALGQGTVNFPGVLRVLRAGGYDNVICVELDFNRVNNYESAYTSRKYIYDVL
                     GL"
BASE COUNT          632 a          922 c          922 g          541 t
ORIGIN      
        1 cgcatcatcc gcagcctgaa agaaaagggc atcgcggtga tcttcatcac ccaccggttg
       61 gacgagatct tcgccatctg cgaccgcatg acggtgctgc gcgacggctg ctatatcggc
      121 acccgcgacg tgaaagacgt caccaagagc gagatgatcg ccatgatgat cggccgcgag
      181 ctgaagcagc agttcccaga gcgtcacgcc gagatcggcg aggagtgcct gcgggtcgag
      241 catatgtccg acggcggccg gcacgtcaag gatgtctctt tcagcgtgcg caggggcgag
      301 gtgctgggct ttgcgggcct ggtgggcgcg ggccgcaccg agacgatgcg gctgatcttc
      361 ggcgccgacc acaaggcgcg gggcaaggtc tttatcgacg gcagagaggt gtccatcgcc
      421 agccccaggc aggcggtggc ccaccgcatc ggctttgtca ccgagaaccg caaggaggag
      481 ggccttttcc tcagcctgcc ggtgcgcaac aacatcgtgg ccgtggccat cgacaagatc
      541 aaatcctttc atatcctgaa tttcaacaga gaaaaagaga tcagcgacaa gtatgtgcgc
      601 gacctgcaga tcgccacccc gtccatcaac cagcgcgtgg tctttttgag cggcggcaac
      661 cagcaaaagg tcgttttggc aaagtggctg ctgtccgatt cccaggtgat gatcctggac
      721 gaacccaccc gcggcatcga cgtgggcgca aagcgcgaga tctatgagat catcaaccag
      781 ctggcggccc agggcaaggc catcatcgtc gtctcctccg agatggaaga gatcatgggc
      841 atcagcgacc ggatcctcgt catgcacgag ggcgaaattt cgggcgaggt caaaaaagag
      901 gacttctccc agagcaccat tactgaatac gcggttggag gagcataaaa tgggagacaa
      961 aaacgcaatc aaggaaaaga cttcattcgg ccagaagctg aataaattca ccgacgccat
     1021 gggcgtatgg ctggtggtgc tgttgttctt catcatcctg tcgctggcga cggataactt
     1081 cctcacctac gccaacctca ccaacgtggt gcgccagatc tgtgtcaacg cgctggtggc
     1141 gctgggcgcg tcctacgtca tcctgggcgg cgagatcgac ctgtccacca gcaacatgtg
     1201 cgcgttcgcg ggctgcggcg ccgcgctgct gatgcgggcc ggggtcaaca tgtggctggc
     1261 gatgctcatc atgctggtca tcggcgccct catcggctgc ctgaccggcg tcatcgtcaa
     1321 ctatctcaag atcccctctt ttatcgccag cctgggcatg cagtacgtca tcctgggcac
     1381 cacgctgctg ttgaccaaca gcgagcccat ctccggcctg ccggagggct ttatgacgtt
     1441 cggccgcggc tatgtctttg acgtcatccc ggtgccggtc atcatcctgg cgatcatgtt
     1501 cgccgtcggc ggcttcgtgt tcaagtacac ctctttcggc cgcagcgtca tctcggtggg
     1561 tgagaacatc acggccgcaa agctgtccgg catcaacgtc tcccgcacca aggtggccat
     1621 cttctccatc ggcggcttca gcgcggccct ggcgggcatt gtgctggcca gccgcctgtc
     1681 ctccggccag cccatggccg gcaccgacat cggcctgcag gccatcgcgg cggtctatgt
     1741 gggcggcacc agcaagggca gcgccctcaa cgtgctggcc ggctcgctgg cgctgggcct
     1801 ggtcaacaac ggcctcaacc tgctggaggt gaacgcctac tggcaaaagg tggccctggg
     1861 tgtcatcatc atctttgcgg tgctgatgga tcaggtgcgc gccagcaaaa cggcaaagaa
     1921 atagcagcgc cacaaaggag gaataccatg aagatcgcct ataccgcctg gacctggctg
     1981 caggacgagt acaacgcctg ggccccgatg agcgacaaac ccaagcgcga ctttgaggcc
     2041 tcgctgcggg acctgcacgc ctgcggctac cgctatctgg aggatttcaa catcgtcgcc
     2101 gacatcttcg acggctgccc cgaggagttt gcggccctga cccgcaagta tgaaatcgag
     2161 ttcgtcaacc tctatcacta cctgaccgcg gattttgagt acgaccgcaa aaagaccgag
     2221 cagtgcatcg cctttatgaa agcgcagggc accacccaga tgaacctgga gccgccgcgc
     2281 aaggcgccgg gccaggtgat cggggagaaa gagctggccg aagtggcgca gcactgcgat
     2341 atcctgggcg ggatgtgccg cgacgcgggc atcagcctgg cgctgcaccc ccactggggc
     2401 acttacgtgg agcgggagga tcagatcgac tattttctcg cccacacccg gcgggactgc
     2461 gtgggcctct gcctggacac cgcccatacc gtgctctgcg gcatggaccc ggaaaagctg
     2521 ttcgccaagt atctggccca gggcgtgctg aaatatgtcc acttcaagga catcgacccc
     2581 gacgagacgg cccatccgga gtatccgccc cgccgcttcc gggcgctggg gcagggcacg
     2641 gtgaactttc ccggcgtgct gcgggtgctg cgcgcgggcg gctacgacaa cgtcatctgc
     2701 gtggagctgg atttcaaccg ggtgaacaac tacgagtccg cctacacctc ccgcaagtac
     2761 atttacgacg tgctgggcct gtgagacggc ggcccggcaa aagctaaaaa acgactgaga
     2821 acaaaaaagg agtgtatcga catgtcaaga aaagcgatga tcttccgtgg cggctgggac
     2881 ggccacgagc cgaaaaaggt gagcgagcgg ttcaaaaagc tgctggaaaa agaggacttt
     2941 gaggtggata tcttcgacac ccaggagtgc ctggccgaga ccgagcccct gctcaagtac
     3001 gacctcatca tccccat
//
LOCUS       DBMDYA010000336         3015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7292_length_3015_cov_1.668840, whole genome shotgun sequence.
ACCESSION   DBMDYA010000336 DBMDYA010000000
VERSION     DBMDYA010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_3015_cov_1.668840"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZPFK_08635"
     CDS             <1..289
                     /locus_tag="ACZPFK_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406417.1"
                     /translation="LLEFMFRRRDSGLEDALMHDALALGAALCPECLTCKDCFVDVEC
                     FGQYTRGHTAVDLKGRSGKPANVSVALGIDVPRFRRWLVDAVKNSAKLGDK"
     gene            286..1644
                     /locus_tag="ACZPFK_08640"
     CDS             286..1644
                     /locus_tag="ACZPFK_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406418.1"
                     /translation="MSNTLIRDKSFYLNIFTLTFPIMLQQLLRISVNTVNSIMLGGID
                     QLQMSAVSQADQVFFIFYTICNGFAVGCCVLVAQYWGRQDVNAIKTVLAIALRAIAVF
                     GLIVTVLVMLFPTFFMRIYSSDPALIELGAGYLRLVALMYTPCAISVMLFAGCRGVEQ
                     VRIILATNIVSYSVNIFLDYCLLFGKFGFPKLGITGIAIGTIIARLVELVFCGVFVLK
                     FEQRIGFRLPDLLRKDKQLSKDFVRVASPIVAHEIIWSFGTSAANMITGQLGTSVVAG
                     YNVTVVLYDLCASVGNGFLNACSVVIGKTIGSGDRDKVKQQARTILLMGLGIGLVLGA
                     VTIPLRGPFLSLYNLEPDAVGYARQFMMVIALIWPFSMLEMVGMIAILRAGGDGKTGF
                     YTDIVAMWLTTIPLAWLAAFVLKWPAVAVVAIIKTTIIIEAVVGIIRVLSMRWIKDLT
                     RH"
     gene            1710..2858
                     /locus_tag="ACZPFK_08645"
     CDS             1710..2858
                     /locus_tag="ACZPFK_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1406419.1"
                     /translation="MKKFASILLVAAMALSLVACGGGDSSSASTSTPAAGGSSTAAST
                     PAEGGKGKIAYIVGNLGDKSFSDSGEAGMNVLRGEGWDVKTFELGDATKADKYEDGIL
                     DALDQGYEYIVGSSTYLEIMIKLAPDYPEVKFVGFDEKKEDADLPENVTCIFYAQNEG
                     SYLVGMMAAAMSKTGTVAVDVGVENPVINDFVTGYIQGVMDWNEANGTDVKVVKAAVD
                     SWTDPAKMKQLCLDQARNNNADVFYQVAGGSGDGLFEACVESKTWAIGVDSDQYQMYK
                     DGENPEKADVILTSMLKEVGNSFVSLFHQIEKGDTDSIWGHTITLGLAQDSVGYVDNA
                     FFQENVPEEIRTAMSDAAAKVKAGDLKVKSYYDFADEAEYNAFVEAAK"
BASE COUNT          583 a          918 c          903 g          611 t
ORIGIN      
        1 cctgctggag ttcatgttcc gccgccggga cagcgggctg gaggacgcgt tgatgcacga
       61 cgcgctcgcc ctgggcgcgg cgctctgccc cgaatgcctc acctgcaagg actgctttgt
      121 ggatgtggaa tgcttcgggc agtatacccg cggccacacc gccgtggacc tgaaaggccg
      181 cagcggcaag cccgccaatg tatcggtggc gctgggcatc gatgtgcccc gcttccgccg
      241 ctggctggtg gacgcggtga aaaacagtgc aaagctgggt gacaaatgag caataccctg
      301 attcgggata agagctttta tctcaacatt tttaccctga cctttcccat catgctgcag
      361 cagctgctgc gcatcagcgt gaacaccgtc aacagcatca tgctgggcgg catcgaccag
      421 ctgcagatgt cggcggtcag ccaggcggat caggtgttct tcatctttta cacgatctgc
      481 aacggcttcg cggtgggctg ctgcgtactg gtggcccagt actggggccg tcaggacgta
      541 aacgccatca aaaccgttct ggccatcgcg ctgcgggcca tcgccgtctt cggcctcatc
      601 gtcacggtgc tggtgatgct gttccccacc tttttcatgc gcatctacag cagcgacccg
      661 gccctgatcg aactgggcgc agggtatctg cgtctggtgg ccctgatgta caccccctgc
      721 gcgatcagcg tcatgctctt tgcgggctgc cgcggtgtgg agcaggtaag gatcattttg
      781 gcgacaaata ttgtttccta ttctgtgaat attttcctgg attattgcct gctttttggc
      841 aaattcggtt ttccgaaact cggaattaca ggaattgcca ttggaaccat cattgcacgt
      901 cttgtagagc ttgtcttttg tggtgtattc gtgttaaaat ttgaacagag gatcggcttt
      961 cgtctgccgg acctcctgcg caaggacaag cagttgagca aggacttcgt ccgcgtggcc
     1021 tcgcccatcg tggcgcacga gatcatctgg tctttcggca ccagtgccgc caacatgatc
     1081 accggccagc tgggcaccag cgtggtggcg ggctacaacg tcaccgtggt gttgtacgat
     1141 ctgtgtgcca gcgtgggcaa cggcttcctc aacgcctgca gtgtggtgat cggcaagacc
     1201 atcggcagcg gcgaccggga caaggtcaag cagcaggcca gaacaatcct gctgatgggt
     1261 ctcggcatcg ggctggtgct gggcgcggtc acgatcccgc tgcgcgggcc gttcctctcg
     1321 ctgtataacc tggagcccga cgcggtgggc tatgcccgcc agtttatgat ggtcatcgcc
     1381 ctgatctggc ctttctcgat gctggagatg gtgggcatga tcgccatcct gcgggccggc
     1441 ggcgacggca agaccggttt ttacaccgat atcgtcgcca tgtggctcac cacgatcccc
     1501 ctggcctggc tggcggcgtt tgtgctgaaa tggcccgccg tggcggtggt ggccatcatc
     1561 aagaccacca tcatcatcga ggcggtcgtc ggcatcatcc gcgtgctgtc catgcggtgg
     1621 atcaaagacc tgacccgcca ttgagttcgg aacccgctct ccaccccgga gagattaata
     1681 gagttcaaaa aagcaaaagg agagaaaaca tgaaaaagtt tgcatccatc cttctggtag
     1741 ctgcgatggc tctgtcgctg gtcgcctgcg gcggcggcga ttcgtcctcc gcttccacct
     1801 ccacccccgc ggccggcggc tcgagcaccg ctgcctccac ccccgctgag ggcggcaagg
     1861 gcaagatcgc ctatatcgtc ggcaacctgg gcgacaaatc cttctccgac tccggcgagg
     1921 ccggcatgaa cgtcctgcgc ggcgagggct gggacgtcaa gacctttgag cttggcgacg
     1981 ccaccaaggc cgacaagtac gaggacggca tcctggacgc tctggatcag ggctacgagt
     2041 acatcgtcgg ctcctccacc tatctggaga tcatgatcaa gctggctccc gattatcccg
     2101 aggtcaagtt cgttggcttt gacgagaaga aagaggacgc tgacctgccc gagaacgtca
     2161 cctgcatctt ctacgcccag aacgagggct cctacctggt cggcatgatg gccgccgcga
     2221 tgagcaagac cggcaccgtc gccgttgacg tgggcgtcga gaaccccgtc atcaacgact
     2281 tcgtcaccgg ctacatccag ggcgtcatgg actggaacga ggccaacggc accgacgtca
     2341 aggtggtcaa ggccgctgtc gacagctgga ccgaccccgc caagatgaaa cagctctgcc
     2401 tggaccaggc ccgcaacaac aatgccgacg tcttctatca ggtggccggc ggctccggcg
     2461 acggcctgtt cgaggcctgt gtcgagagca agacctgggc catcggcgtt gactccgacc
     2521 agtatcagat gtacaaggac ggcgagaacc ccgagaaggc cgacgtcatc ctgacctcca
     2581 tgctgaaaga agtcggcaac tcctttgtct ccctgttcca tcagatcgag aaaggcgaca
     2641 ccgactccat ctggggccac accatcaccc tgggcctggc gcaggattcc gtgggctacg
     2701 tggacaacgc tttcttccag gagaacgtgc ctgaggagat ccgcaccgcc atgtccgacg
     2761 ccgccgccaa ggtcaaagcc ggcgacctga aggtcaagag ctactacgac tttgccgacg
     2821 aggccgagta caacgccttt gtcgaggctg cgaaataagc tttcgctaaa gtttaaacgg
     2881 taatgctggg atgacccgga tgctttctca gcctccgggt catctttgca agaggggcct
     2941 gggacgggca ataaaaacgg cgcacgccgc cgttttggcc cgccgggccc cgggagccgc
     3001 ccgctttgca ggggg
//
LOCUS       DBMDYA010000337         3008 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7316_length_3008_cov_1.528724, whole genome shotgun sequence.
ACCESSION   DBMDYA010000337 DBMDYA010000000
VERSION     DBMDYA010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7316_length_3008_cov_1.528724"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="ACZPFK_08650"
     CDS             <1..191
                     /locus_tag="ACZPFK_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406420.1"
                     /translation="TWDTKDEFWYIRGLRALDYGLFEPGQEIQDCVMGAATGRLYYNA
                     GMGYYEDLWLEGVEPGGG"
     gene            618..965
                     /locus_tag="ACZPFK_08655"
     CDS             618..965
                     /locus_tag="ACZPFK_08655"
                     /inference="COORDINATES: protein motif:HMM:NF049338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7674 family protein"
                     /protein_id="HIR1406421.1"
                     /translation="MTSKELNLKLVRAIPGIAPSYQQEVEWQEGDETGSHVVYEDVLA
                     PYIRQAAGAGDRDGLDEIFGFIEQLLETGDGYAEEVVALSVLEALGQEEKIRNACLPF
                     MKTKTKKLFSELG"
     gene            1177..1488
                     /locus_tag="ACZPFK_08660"
     CDS             1177..1488
                     /locus_tag="ACZPFK_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406422.1"
                     /translation="MKTTFGDVSLISNDDYKTPKEKRSKLPEDAFLYWRYYLEIEPLP
                     AISRETYVEKISRVLRGLGRLGIGAVAACDFEEELPGAEKRNSDRPDGTARGASSVGQ
                     L"
     gene            1499..1972
                     /locus_tag="ACZPFK_08665"
     CDS             1499..1972
                     /locus_tag="ACZPFK_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406423.1"
                     /translation="MDMILDVLAPEDQIKNLPLLELKAEWGGEAVSFTPARYSYRSVL
                     IFHENKDYAYYRRQCGKEVQLGEKGLALTPKGEVLSELEFLVNREKEAVEEHPLVMFL
                     RELYDALERFAVLLFREEEYLDEKFIVTSADELIGRFCKSLEWEKPRGGLFVKGR"
     gene            2246..2467
                     /locus_tag="ACZPFK_08670"
     CDS             2246..2467
                     /locus_tag="ACZPFK_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406424.1"
                     /translation="MVFLLHVEDGLLKELEVYNADLSEVEIEYPITKWNTEVRVEKVV
                     EENSRRYGVPPEMNGKSLHSLSELETVEE"
BASE COUNT          754 a          653 c         1007 g          594 t
ORIGIN      
        1 atacatggga cacaaaggac gagttttggt acattcgggg attgcgggcg ttggattatg
       61 gcctttttga gccggggcag gaaattcagg actgtgtgat gggcgcggcg accggcaggc
      121 tatactataa cgcagggatg ggctactacg aagacctctg gcttgagggt gtggaaccgg
      181 gcggcggctg agcgtgtcgc tcgcgcaaag gatcattttg ggaatccgcg tttttgagac
      241 ctgcaaaatt gagataagga ccggcggaga gggtgctctc cgccggtcct ttttgcctgc
      301 gacggttttt cactggcccg gcggcaagag ggcgagcggc ggcagccgcc ggagaggagc
      361 gcgccccaca ccctgccgcg accgtgggag aaaagggaac aatggccggt ggaaagcccg
      421 cgggagcagg aaaaaatgtt gcggctgctg agggaaaagg gattgcaaac gccgtttgtg
      481 ggtgatacgc ttaacccaaa gagcggtgat ggccgggctt tgctgcggcc gaggcacgcc
      541 gctattgggc tgcggccggg acgcaatcag gatgaaaaaa cagggaaagc cttgttgggg
      601 cgatcggggg gataggtgtg acatcaaaag aactgaatct gaagctggtg agggcgatcc
      661 cggggatagc gccctcttac caacaagagg tcgagtggca ggagggggac gagaccggca
      721 gccatgtggt ctatgaagac gtcctggcgc cttatatccg gcaggcggcc ggcgccggcg
      781 atcgggacgg tttggacgaa atttttggat tcatagagca gcttcttgaa acgggggacg
      841 gttatgcgga ggaggtcgtt gcactcagcg ttctggaagc tttggggcag gaagaaaaaa
      901 taagaaacgc ctgtctccct tttatgaaaa caaaaacaaa aaagctgttt tcagaactgg
      961 gataacgcaa aagcaagccc gcttaacaga gaccggcaaa gggctgaaaa aaacgggcag
     1021 gggccgcggc gacggcgcgg gccctgtggg ggaagcgaat tgggaaccgg gaatttatat
     1081 tgtcaaattt atctggactg tgaaaaaagt caggatgagc ttcaaaaact ggtggctcat
     1141 ctggccgatg gggttccggg gagatggaag aaagaggtga aaaccacttt tggagacgtc
     1201 tcccttatat ccaacgacga ctataaaacg ccgaaagaaa aaagatcaaa attgcccgag
     1261 gacgcttttt tgtactggag atattatctg gagatagagc ctctgcccgc gataagccgg
     1321 gagacctacg tggaaaaaat atcccgggtg ctgcgcgggc tggggcggct ggggatcggc
     1381 gctgtcgcgg cctgcgactt tgaggaggag ctgccggggg cagaaaaaag aaacagcgac
     1441 cggccggacg gaacggcgcg cggggcctct tccgtcggtc aactataagg aaaaagacat
     1501 ggatatgata ctggatgttt tagcgcccga ggaccagata aaaaatcttc ctttattgga
     1561 gctgaaagcg gaatggggcg gggaggccgt ttctttcacc ccggcgcgct attcttaccg
     1621 ctctgtttta atatttcatg aaaacaaaga ctacgcctac tatcgccgcc agtgcggaaa
     1681 agaagtgcag ctcggcgaaa aagggctggc actcaccccg aaaggagaag tgctttccga
     1741 actggaattt ctcgtaaaca gggaaaagga ggccgtcgaa gagcatccgc ttgtgatgtt
     1801 tttgagagag ctttacgatg ccttggagcg ttttgcggtg cttctttttc gggaagaaga
     1861 gtatttggat gaaaaattta ttgtaacaag cgcagacgag ttgatcggcc gcttctgcaa
     1921 gagccttgag tgggaaaagc ctcggggagg actgtttgtg aaagggcgat aaaagcggcc
     1981 cgttgatgcg aaaagcgggg gagggaatat gagaagagat aaattgacct ccaaagaaaa
     2041 aaagtggatg atgtatctta taaaaaagat ggagaaaccg gagaaaaaga tcgctcttcg
     2101 acagttgaga aaagcgcgga tcaagaggga atatgatccc tattacattt ttgtggattt
     2161 tgttctgccg caaaatgtag agcggctgcc gttttcaaat ggcgttccgg cagagatgtg
     2221 ggcgaagtgg gtgggccctg cgccgatggt ttttcttttg catgtggaag acggcctgct
     2281 gaaagaattg gaggtataca atgcggacct ctcggaggtg gagatcgagt accccatcac
     2341 aaagtggaac acggaagtgc gggtcgaaaa agtggtggaa gagaacagcc gccggtacgg
     2401 cgtgccccct gaaatgaatg gaaaaagcct acattctctt tccgagcttg agaccgtgga
     2461 agaataaacc tgttgaaaag cggccggcgc tgttgagtga gcgccggccg cttttggttt
     2521 catccctcgg cggggggcgc ttgcggcttt ccggcaggcg gggggccgcc ccgggggctg
     2581 gacaggcggg ggcttgagcg cgctgctcgc gcgaaaggcc gttttggaca tccgtatttt
     2641 tgagagctgc aaaattgaaa aaaggggcct gcggagaggg tgctttccgc gggccccttt
     2701 ttttaacttt tcgttaagga aatcggcttt ttgagcgcgc aaagcggcct gtgaagcggc
     2761 tttgagggca tttttgaggg gcaaaaaagt tggtgcgtta tccaagctcg gttagtatac
     2821 aattttaagt acagggcaag gagagcacgc gcgccgcagg gcgcacgggc ccgcagaggc
     2881 cctgcgggat gccggggaac gccgcgggag gcgggccccg gcccggacgc agaccggcaa
     2941 aagcagatat gccccgagag gggcagcaga caacgccctc acgtgggcga gagatacagg
     3001 gaggtgcg
//
LOCUS       DBMDYA010000338         2992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7350_length_2992_cov_1.301280, whole genome shotgun sequence.
ACCESSION   DBMDYA010000338 DBMDYA010000000
VERSION     DBMDYA010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_2992_cov_1.301280"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="ACZPFK_08675"
     CDS             complement(<1..523)
                     /locus_tag="ACZPFK_08675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1406425.1"
                     /translation="MVKKIAALCAALLLALGAAAPVSAGEAAVPPPLSAGAACLMDAS
                     TGQILYEKNMDAQLPPASITKIMTVLLGVENGNWDDVVTMTDASVFSVPRSASHLALT
                     PGEQLTLEQALMGAMLPSANDAANGVAETIGGTIENFVEMMNARAAELGARNTHFVNA
                     NGLDDPAHLTTARD"
     gene            1233..1910
                     /locus_tag="ACZPFK_08680"
     CDS             1233..1910
                     /locus_tag="ACZPFK_08680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1406426.1"
                     /translation="MIRSVIFDMDGLLIDSECLSVSGWRQAFREAGLPFSEQLIASCR
                     GTGREDMRRRFEAYFAGRAADFDALLRRKNELADQVTAREGAHPKVGALTLLGWLRDQ
                     GYAVALATSTGEAVASEKLRGIGFYDYFDHYVYGSMITRYKPAPDIFLRAIELLDCPP
                     QECLVLEDSPNGIRAAHAAGAKPMMIPDLTQPDEELKKLLWAQGTTLLDVIPLLEDDR
                     AGEGAAL"
     gene            1907..2914
                     /gene="asnA"
                     /locus_tag="ACZPFK_08685"
     CDS             1907..2914
                     /gene="asnA"
                     /locus_tag="ACZPFK_08685"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1406427.1"
                     /translation="MSNTVIPAGYRSRLTLYETQRAIGLIKNLFLNRLSTALHLKRVT
                     APLFVDPASGLNDDLNGVERPVSFDVPASGSDAQVVHSLAKWKRFALAQYDFHVGNGL
                     VTDMNAIRRDEELDNLHSIYVDQWDWEKVITREQRTIPYLEQTVNGIVGAICGTLYDL
                     KDYFPALDVELCREVTFLTSQELEDRYPALTPKQREDAVVREHHTVFLEQIGGKLRSG
                     SPHDGRAPDYDDWGLNGDLLFYDPLLDRALEISSMGIRVDAETLDRQLTLAGCDGRRA
                     LPFHKALLAGELPLTIGGGIGQSRLCMLLLGKAHIGEVQVSLWDEETVAACRAGGIQL
                     L"
BASE COUNT          516 a          953 c          961 g          562 t
ORIGIN      
        1 tatcccgcgc ggtggtgaga tgcgcgggat cgtccagccc gttggcgttg acgaaatggg
       61 tgttgcgcgc ccccaactcc gccgcccggg cgttcatcat ctccacaaaa ttttcgatcg
      121 tgccgccgat ggtctcggcc acgccgttgg ccgcgtcgtt ggcggagggc agcatcgccc
      181 ccatcagcgc ctgctccagc gtcagctgct cgccgggtgt gagggccagg tggctggcgc
      241 tgcgcggcac gctgaacacc gaggcgtcgg tcatggtgac gacatcgtcc cagttgccgt
      301 tttccacccc cagcagcacc gtcatgattt tggtgatcga ggcgggcggc agctgggcgt
      361 ccatattttt ctcgtagagg atttgcccgg ttgaggcgtc catgagacag gcggcgccgg
      421 cggagagagg cggcgggacc gccgcctccc cggcggacac cggcgctgcg gcgcccagcg
      481 ccagcagcag agcggcgcac agggccgcga tttttttgac caagagaggc tcacaccttt
      541 gcggattttt tgagcggccg ggtgtcggcc tgcgcgggcg cgcaggcagc cgccgcaggg
      601 ggcggcggcc ccgtgccgcg ctcttttagt atgtctggag gcgcgcggtt tcattcgcgg
      661 ttttccgccc ggttttcgcc tcacaacctg tctgtctgcg tcgaaagatc ggtctgtttg
      721 tcacttattc taacacagcc cgtccggcaa gaaaagcctc ggcggccaca aatcctgtga
      781 aagtttgccc gctccgccgc cccgtcaatt tttatagttt tgcatcgcaa taaaaaagct
      841 catcttaacg gctccggcca caggaccggc cggatggccg aaaaaacaac ttatttcaat
      901 ggccccgttt ctcgccgctg ccgcggcaac cgaaacggat ggtcgaaaaa acaacttatt
      961 tcaatggccc cgtttctcgc cactgtcgca gcaaccgaaa cggatggccg caaaaaatag
     1021 cctgcggcta ttttttattg cttcgcaata aaaaacgact tattcctatg gccgtggccc
     1081 gaggacgggc cacatggccg aaaaaatagc ctccgcttcg ctgcggctat tttttatttg
     1141 ccatgccggg cgcgatgcgc tataatcgtt ttgtgcgagc tgcggcgggc gcgccgcggc
     1201 tgtctttgag gggaaagggg aatctttact gcatgatcag atctgtcatt tttgatatgg
     1261 atgggctgct catcgactcg gaatgcctgt cggtgagcgg ctggaggcag gcctttcggg
     1321 aagcgggcct gcccttttcc gagcaattga tcgccagctg ccgcggcacc gggcgcgagg
     1381 atatgcgccg ccgctttgag gcctattttg cgggccgggc cgccgacttc gacgctctgc
     1441 tgcgccgcaa aaacgagctt gccgaccagg tcaccgcccg cgagggcgcc caccccaagg
     1501 tgggggccct gaccctgctg ggctggctgc gcgatcaggg ctacgccgtg gccctcgcca
     1561 cctctaccgg cgaggccgtc gcctcggaaa agctgcgcgg catcggcttt tacgactatt
     1621 tcgaccacta tgtctacggc agcatgatca cccgctacaa gcccgcgccg gatatctttt
     1681 tgcgcgccat cgagctgctg gactgcccgc cgcaggagtg cctggtgctg gaggattccc
     1741 ccaacggcat ccgcgcggcc catgccgccg gggcaaaacc gatgatgatc cccgacctga
     1801 cccagccgga cgaggagctg aaaaagctgc tgtgggcgca gggcaccacg ctgctggacg
     1861 tcatccccct gctggaggac gaccgcgcag gagagggggc ggcactgtga gcaacactgt
     1921 gatcccggcg ggctaccgct cgcgcctgac cctgtatgag acccagcggg ccatcgggct
     1981 gatcaaaaac ctgtttctca accgcctgtc caccgcgctg catctgaagc gggtgaccgc
     2041 gccgctgttt gtggacccgg cctccggcct caacgacgat ttgaacggcg tcgagcggcc
     2101 ggtcagcttt gacgtgcccg cctccggttc ggacgcgcag gtggtgcact cgctggcaaa
     2161 gtggaagcgg tttgcgctgg cgcagtacga ttttcacgtg ggcaacggtc ttgtgaccga
     2221 catgaacgcc atccgccggg acgaggagct ggacaatctg cactcgatct atgtggacca
     2281 gtgggattgg gaaaaggtca tcacccgcga gcagcgcacc atcccctatc tggagcagac
     2341 ggtgaacggc atcgtgggcg ccatctgcgg caccctgtat gatttaaagg attacttccc
     2401 ggcgctggat gtggagctct gccgcgaggt cacctttctc accagccagg agctggagga
     2461 ccgctacccc gccctgaccc cgaagcagcg ggaggacgcg gtggtgcggg agcatcacac
     2521 cgtctttttg gagcagatcg gcggcaagct gcgctcaggc agcccccacg acggccgcgc
     2581 gccggactat gacgactggg gcttgaacgg cgacctgctg ttctacgacc cactgctgga
     2641 ccgggcgctg gagatctcgt ccatgggcat ccgggtggac gccgagacgc tggaccgcca
     2701 gctcaccctg gcgggctgcg acgggcggcg cgctctgcct ttccacaagg cgctgctggc
     2761 gggcgagctg ccgctcacca tcggcggcgg catcggccag agccgtcttt gcatgctgct
     2821 gctgggcaag gcccacatcg gcgaggtgca ggtctcgctt tgggacgagg agacggtggc
     2881 cgcctgccgc gcgggcggca tccagctgct gtgaggcatc cgccccgacg cggcggaccc
     2941 cggagcccaa gatcaggata tggaaaaaca ggaggcagct gagctgcctc ct
//
LOCUS       DBMDYA010000339         2991 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7352_length_2991_cov_1.380277, whole genome shotgun sequence.
ACCESSION   DBMDYA010000339 DBMDYA010000000
VERSION     DBMDYA010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_2991_cov_1.380277"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="uppS"
                     /locus_tag="ACZPFK_08690"
     CDS             <1..626
                     /gene="uppS"
                     /locus_tag="ACZPFK_08690"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR1406428.1"
                     /translation="RTAGHTKGAQVFQDMVKYCKQLGAEAVTFYAFSTENWKRPRSEV
                     DAIMKLLKEYLNRAFDYKKEDNRIIVLGDKTPLSDDIKKLMAEIEDGTRENKGMTINV
                     AINYGGREEITRAARALARQVQQGVLLPEEIDEQRLADQLYTAGQKDPDFILRPSGEK
                     RLSNFLLWQSAYAEFVDMEVLWPDFTRRDLDAAIAEFRRRNRRFGGI"
     gene            687..>1273
                     /locus_tag="ACZPFK_08695"
     CDS             687..>1273
                     /locus_tag="ACZPFK_08695"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1406429.1"
                     /translation="MKTRIITSLVGIPILFLALYFYQTFFFNILIAAICMIAFYEIIS
                     AFKVKNAFWLYLAIVPMELILLFSDRNSFRRFVPLILFLFIVYIAVCVIFEFKELTFA
                     QVSEVILFCGIVMFGFYSIISFKILFPFNEYHYDALYLILLGFSFAWGGDSAAYFTGR
                     ALGKHKLAPKVSPNKTVEGAVGGVVGSMALGCLFT"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1284..1563
                     /locus_tag="ACZPFK_08700"
     CDS             <1284..1563
                     /locus_tag="ACZPFK_08700"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1406430.1"
                     /translation="LYSLLLPVVEGPSAAATIDARCYLLVCGVGAVASVLGILGDLFT
                     SAIKRQCGIKDYGSIFPGHGGILDRFDSVLLVMPFVSLVSRVLPFITR"
     gene            1581..2738
                     /gene="dxr"
                     /locus_tag="ACZPFK_08705"
     CDS             1581..2738
                     /gene="dxr"
                     /locus_tag="ACZPFK_08705"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1406431.1"
                     /translation="MTKRITLLGSTGSIGTQSLEVVRAQGYEVFGLAANRSAELLLRQ
                     IAEFHPRAVAVVDAAAAQQVQSGLDGMSDAPLLLKGREGLAELAAMPGADIVLNAVVG
                     VAGLAPTLAAIESGKDLALANKESLVTGGALVTDAVRRRGVHLLPVDSEHSAIFQCLQ
                     DQHSAKRLSKILLTASGGPFFGKTAEQLRSMTAADALRHPNWSMGSKITVDSASLMNK
                     GLELIEAVWLFGLPPEKVQIVVQRQSIIHSMVQFADNSVLAQLGVPDMRIPIQYALTW
                     PDRAPGPAPELDFTTLGTLTFDVADEGTFRCLAAAKQAIALGGLVPTALNGANEKAVE
                     LFLQGRIGFLEIGRMAELAVERFASAVGAYTVQDVFACDAQARRMVEEEAS"
     gene            2809..>2991
                     /locus_tag="ACZPFK_08710"
     CDS             2809..>2991
                     /locus_tag="ACZPFK_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1406432.1"
                     /translation="MSLFITILASVLVFGAVIFIHELGHFTTAKLCGIKVNEFAIGMG
                     PTLLKRQKGETTYALRA"
BASE COUNT          540 a          892 c          953 g          596 t
ORIGIN      
        1 cgcgcacggc ggggcacacc aagggcgcgc aggtctttca ggacatggtg aaatactgca
       61 agcagctggg ggccgaggcg gtgacctttt acgccttttc caccgagaat tggaagcgcc
      121 cccggagcga ggtggacgcg atcatgaagc tgctcaagga gtatctgaac cgcgcgttcg
      181 actacaaaaa agaggataac cgcatcattg tgctggggga caagacgccg ctcagcgacg
      241 acatcaaaaa gctgatggcc gagatcgagg acggcaccag ggagaacaag gggatgacca
      301 tcaacgtggc catcaactac ggcggccggg aggagatcac ccgcgccgcc cgcgccctgg
      361 cgcggcaggt acagcagggc gtccttttgc ccgaggagat cgacgagcag cgcctcgccg
      421 accagctgta cacggccggg caaaaggacc cggattttat cctgcgcccc agcggggaaa
      481 aacggctgtc caactttttg ctttggcaga gcgcttacgc cgagtttgtg gatatggagg
      541 tgctctggcc cgatttcacc cgcagggacc tggacgcggc catcgccgaa ttccgccgcc
      601 gtaaccggcg cttcggcggg atctgagagg gttctgggcc aaagcgcaca aaatttcaag
      661 ttcgcagcga aaggctggtc ttactgatga aaacaaggat catcacgtct ctggtgggca
      721 tccccatcct ttttctcgcc ctttattttt accagacttt ctttttcaat atcctcatcg
      781 ctgccatctg catgatcgcc ttttatgaga tcatcagcgc tttcaaggtg aaaaacgcct
      841 tttggctcta tctcgccatc gtgccgatgg agctgatcct gctgttcagc gaccggaaca
      901 gctttcgacg cttcgtgccg ctgattttgt tcctctttat cgtgtatatc gcggtctgcg
      961 tgatcttcga gttcaaagag ctcacctttg cgcaggtgag cgaggtgatc ctgttctgcg
     1021 gcatcgtgat gttcggcttt tattccatca tctctttcaa aatcctgttc ccgttcaatg
     1081 aataccacta tgacgcgctc tacctgatcc tgctcggctt cagctttgcc tggggcgggg
     1141 acagcgccgc ctacttcacc ggccgcgccc tgggcaaaca caagcttgcc cccaaggtca
     1201 gccccaacaa aaccgttgag ggggccgtgg gcggcgtcgt cggcagcatg gcgctgggct
     1261 gcctgttcac ctgnnnnnnn nnngctgtat tcgctgctgc tgccggtggt ggaggggccg
     1321 tccgcggcgg cgacgatcga cgcgcgctgt tacctgttgg tctgcggcgt cggcgcggtg
     1381 gcgagcgtgt tgggcatcct gggcgatttg ttcacctccg ccatcaagcg ccagtgcggc
     1441 atcaaggatt acggctcgat ttttccgggc cacgggggca tattggacag gtttgacagc
     1501 gtactgttag tcatgccctt tgtctcgctg gtctcccgcg tgctgccctt tatcacccgg
     1561 tgaacaagga gggagagtcc atgacgaaac gcatcacact gctgggctcc acgggctcca
     1621 tcggcaccca gagcttggag gtcgtgcgcg cccagggcta tgaggtgttc ggccttgctg
     1681 caaaccgcag cgccgagctg ctgctgcggc agatcgcgga gttccacccc agggcggtgg
     1741 cggtggtgga tgccgccgcg gcacagcagg tgcagagcgg ccttgacggc atgagcgatg
     1801 cgccgctgct gctgaaaggc cgcgagggcc tggcggaatt ggcggccatg cccggggcgg
     1861 acatcgtgct gaatgccgtg gtgggggtgg cgggtctggc gcccaccctg gccgcgattg
     1921 agagcggcaa ggacctggcc ctggccaaca aggagagcct ggtcaccggc ggcgcgctgg
     1981 tgaccgatgc cgtgcgccgc aggggcgtgc acctgctgcc ggtggacagc gagcactcgg
     2041 ccatttttca gtgcctgcag gaccagcact cggccaaacg gctgagcaaa atactgctca
     2101 ccgcctccgg cgggccgttt ttcggcaaga cggcggagca gctgcgctcc atgacggccg
     2161 cggacgcgct gcggcacccg aactggagca tgggcagcaa gatcaccgtc gacagcgcca
     2221 gcctgatgaa taaggggctg gagctgattg aagcggtctg gcttttcggc ctgccgccgg
     2281 aaaaggtgca gatcgtggtg cagcggcaga gcatcattca ctcgatggtg cagtttgccg
     2341 acaactcggt gctcgcccag ctgggggtgc cggatatgcg catccccatc caatacgcgc
     2401 tgacctggcc ggaccgcgcg cccggccccg cgcctgaatt ggacttcacc actttgggca
     2461 cactgacctt tgatgtggcg gatgagggca cgttccgctg ccttgcggcc gcaaaacagg
     2521 ccatcgcgct gggggggctg gtgcccacgg cgctcaacgg tgccaacgaa aaagcggtcg
     2581 agctgttttt gcagggcagg atcggctttt tggagatcgg ccgcatggcc gagttggcgg
     2641 tggagcggtt tgcatccgct gtcggcgcct atacggtgca ggacgttttt gcctgcgacg
     2701 cccaggcgcg ccgcatggtg gaagaggagg cgtcctgaag ccccaggtac aaaaattttc
     2761 ggcagggcgc ggttttagcc gcgccggccg gttttttaag gagaaaccat gtctttattc
     2821 atcaccatac tcgcctccgt cctcgtcttc ggggccgtga tcttcatcca cgagctgggc
     2881 cacttcacca ccgccaagct ctgcggcatc aaggtcaacg aatttgccat cggcatgggg
     2941 ccgaccctcc tcaagcggca gaagggggag accacctatg ccctgcgggc g
//
LOCUS       DBMDYA010000340         2986 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7365_length_2986_cov_1.450953, whole genome shotgun sequence.
ACCESSION   DBMDYA010000340 DBMDYA010000000
VERSION     DBMDYA010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7365_length_2986_cov_1.450953"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="ACZPFK_08715"
     CDS             <1..581
                     /locus_tag="ACZPFK_08715"
                     /inference="COORDINATES: protein motif:HMM:NF019886.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1406433.1"
                     /translation="HPLSFELYGEDIASGRERDLPDLSGGVDRALLEQELKLCCTNVM
                     LQCLDPESRCAYILGTMFRLDSRVAAEALELTPEAYRQRLSRARKKMAAFLGRYCGLG
                     GGVCACARRVDYAVATHRIDPAAPAFAALSPCELDRALRHIRAMEQLDQLSQVFADLP
                     AYRAPGGAVKQVRALLRSDGFAAAVRGGEVGV"
     gene            578..1219
                     /locus_tag="ACZPFK_08720"
     CDS             578..1219
                     /locus_tag="ACZPFK_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020922.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR1406434.1"
                     /translation="MSMEQMLGYLAALEQNNDRGWFHSHRREYEQARAGFESLVDGLL
                     PLLRRFDPSLPILPARELTFKLMRDTRFGRDKAPYNPAFRAHLGPRGKLPVPVGYYLM
                     LRPGDRSFLGGGLFADMFAGATTRVRDAIAARGEEWAAVLAEPAFAGAFSVGGSTLKK
                     MPRGYDPAHPQAEWLRHKSWIWNFRCRTAGCWTRAFCPGPRRLFTKCSLSTPF"
     gene            complement(1611..2576)
                     /locus_tag="ACZPFK_08725"
     CDS             complement(1611..2576)
                     /locus_tag="ACZPFK_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406435.1"
                     /translation="MYEWQRQIQRIVDEIDRCIGEHCGEALTLRSLACQLGYSEFYTT
                     RKFKEISGMRFRQYLRQRRLAFALKEVRDSEKGFLEIALDHGFSSHEAFTRAFKRAYG
                     VTPSAYRKSPQPVVLRTKIHPFDRYFLGLGEIGMIRSTEEVKIYFVTIPAHKFLHVKN
                     YESNGYWDFWQRQNRIPGQDFETVCGLLDSIQGKLDDEGGGEANGAGGQIMAYFNDPQ
                     GRLCDWGIPRTECYGVRLPSDYAGKVPPQMLLADIPQGEYIVFEHGPFDYEQENRTVE
                     EKIEKAMATFDFAAAGCRLDTTPGRVMYFYFDPKQCFKYIRPVCR"
BASE COUNT          472 a         1023 c          936 g          555 t
ORIGIN      
        1 ctcacccgct cagctttgag ctctacggcg aggacatcgc ctccgggcgc gagcgggacc
       61 tgccagacct gagcggcggc gtggaccggg ccctgctgga gcaggagctc aagctctgct
      121 gcaccaacgt catgctgcaa tgcctggacc cggagagccg ctgcgcctac atcctgggca
      181 ccatgttccg tctggacagc cgggtggcgg ccgaggcgct ggagctgacc cccgaggcct
      241 atcgccagcg cctgtcccgc gcccgcaaaa agatggcggc ctttttgggc cgctattgcg
      301 gccttggcgg cggcgtctgc gcctgcgccc gccgggtgga ttacgccgtc gccacccacc
      361 gcatcgaccc ggccgcccct gcctttgccg cgctgtctcc ctgtgagctt gaccgggccc
      421 tgcggcacat ccgggccatg gaacagctgg accagctctc acaggtcttt gccgacctgc
      481 ccgcctaccg cgcgccgggc ggggcggtga agcaggtgcg ggcgctgctg cgcagcgacg
      541 gctttgccgc ggcggtgcgg ggcggggagg tcggcgtatg agcatggaac agatgctggg
      601 ctatctggcg gcgctggagc agaacaacga ccgcggctgg ttccacagcc accgccggga
      661 gtatgagcag gcacgcgcgg ggtttgaatc actggtggac ggcctgctgc ccctgctgcg
      721 ccggttcgac ccgtcgctgc ccatcctgcc cgcgcgggag ctgactttca agctgatgcg
      781 ggacacccgt ttcggccgtg acaaagcccc ctacaacccg gcgttccgcg cccatctggg
      841 gccgcggggc aagctgccgg tgccggtggg gtactatctg atgctgcggc cgggggaccg
      901 ttcgtttctc ggcggcggcc tgttcgcgga catgtttgcg ggcgccacca cccgggtgcg
      961 ggacgccatc gcggcgcgcg gggaggaatg ggcggcggtc ctggcggagc ccgctttcgc
     1021 cggggctttc tcggtggggg gcagcaccct gaaaaagatg ccgcggggct atgacccggc
     1081 ccatccccag gcggaatggc ttcgccacaa gagctggatc tggaacttcc ggtgccggac
     1141 agccggctgc tggacccggg ctttttgccc tgggccgcgg aggcttttca caaaatgcag
     1201 cctttcaacg cctttttgaa acgcgcgctg gaggggttcg aattgccctg ccgctagcgg
     1261 gcggccatcc ccccgctccg gcccccgccc tggaggagcc agtccctccc tagggagagg
     1321 ctgtgaggcg tgtcttcagc cctgcgaacc gcccaacaca gcgtcttgca tcttctcctt
     1381 tcagagtggc tcagggccgc ccaccaggtg caagccgccg gtgcgccggt cccttttccc
     1441 cgcccgcccc tccggcacgg cttcgtcggg ccgttttccc tctgccgggg gcgcggagac
     1501 gcgcccttgc ccctgtgttc gacaaaagcg gcctccaaaa aggaggccgc tttgttgacc
     1561 cgccatgccc ggcggctctc cggctcgttt cgcgccgtgc ccggcggccg tcatctgcac
     1621 accggccgga tatatttaaa gcactgcttg ggatcgaaat agaaatacat caccctgccc
     1681 ggggtggtgt ccaaccgaca gccggcggcg gcaaagtcaa aggtggccat cgctttttcg
     1741 atcttctcct ccacggtgcg gttctcctgc tcataatcga acgggccgtg ctcaaaaacg
     1801 atatactcgc cctgcggaat atcggccaac agcatctgcg gcggcacctt gccggcgtaa
     1861 tccgacggaa gccgcacccc gtagcactcg gtgcgcggga tgccccagtc gcagagccgc
     1921 ccctgggggt cgttgaaata cgccatgatc tggccgccgg cgccgttggc ctcgccgccg
     1981 ccctcatcgt ccagcttgcc ctggatgctg tccagcagcc cgcagacggt ctcgaaatcc
     2041 tgccccggga tgcggttctg ccgctgccaa aaatcccaat agccattgct ctcatagttt
     2101 ttgacatgta aaaatttgtg cgccgggatg gtcacgaaat aaatcttgac ctcctctgtg
     2161 gaccttatca tgccgatctc ccccaagcct aaaaagtagc ggtcgaacgg gtggatcttg
     2221 gtgcgcagga cgacgggctg cgggcttttg cggtacgcgc tcggcgtcac gccataggcg
     2281 cgcttaaacg ccctggtgaa cgcctcgtga gaggaaaagc cgtggtccag cgcgatctcc
     2341 agaaaaccct tctcgctgtc ccgcacctct ttgagcgcaa aggccagccg ccgctgccgc
     2401 agatactggc ggaaccgcat ccccgagatc tccttgaatt ttctggtggt gtaaaactct
     2461 gaatagccca gctggcaggc gagcgagcgc agcgtaagcg cctcgccgca gtgctcgccg
     2521 atacagcggt cgatctcatc gacgatccgc tgaatttgtc tttgccattc atacatccgc
     2581 ccgtcacctc ctttcaaccg ctctgccccc actataaagg aaacgccgcc cccgccgctt
     2641 gatctcactt gcccttttct ctatcatacc ccttttttat gccccaagac agcctgcatg
     2701 cggggttctg ggggcgcatc ttgccgaggg ggcagagagg ccggacgccc tcttcaatgg
     2761 aatcggcatg tggaatctat gagccaaggc gctgcgctct gccttttgcc cctgcggccc
     2821 gcctcggcgt tttcttgctc caccgccctc cgccctgcgg gagccgccct cccggggagg
     2881 gagacagcgg ggcggcaagg gtgcgcccga ggagcgccgg gcgcggcgca gtgcccaaaa
     2941 aggaaaatcg ctaccttagg accgttatag ttacggccgc cgttta
//
LOCUS       DBMDYA010000341         2985 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7368_length_2985_cov_0.874480, whole genome shotgun sequence.
ACCESSION   DBMDYA010000341 DBMDYA010000000
VERSION     DBMDYA010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_2985_cov_0.874480"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            457..1227
                     /locus_tag="ACZPFK_08730"
     CDS             457..1227
                     /locus_tag="ACZPFK_08730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1406436.1"
                     /translation="MIKNIIFDLGGVVVDYDPKAYLYRQFNDPELEELLYNVIFGSEE
                     WKQLDAGTITRALAEQHMLAAAGRRRYEAQLVLDDWREMMTTRLDTVELITGLKAAGY
                     HIYFLSNLPEDVYQLFTERRRFMQLFEGGIPSFSVKLTKPNRKIFDLMLKTYGLRPEE
                     TIFVDDGKENIATAQKLGLTAIPFKDAMDLHKTLAFLGVAVPAKLRHAPRPKAPKKTG
                     RWLRRKKRAAAPAADAPQETAPPAVDLPLEDDDEDDDI"
     gene            1336..2898
                     /locus_tag="ACZPFK_08735"
     CDS             1336..2898
                     /locus_tag="ACZPFK_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1406437.1"
                     /translation="MNTELIQRALEEVRKSKEDFAAMLEQAELGDSSVQYDLAVRYSE
                     GDGVGKDPERAFYWFSEAALLGDVRAIAALGRCYQFGSGVERDLEKAREQYELAADQD
                     YAPALCDLGLCYENGTGVPRDEAAAAELYARAAEQEYAPGLCCLGLCYEMGTGVEMDK
                     LRATELYAQSAELGYAPAQCNLGFCYLTGIGVPVDADKAVPLFRKAAEQDFPRAIDLL
                     GNCYYEGEGVEQDRQKAVECYARAAGLDYAPAQCNLGLCYELGQGVERDPAKAAAYYI
                     QSAQTGHAPAQCNLAVCYLNGIGVERDEAKAVKWLEAAAAQRFPRALDLLGFCLQNAV
                     GIERDDERAVLCYTRAAEQGYAPAQCSLGLCYENGEAVNVDLPRAAGLYQQAADRGHA
                     PSQCNLAVCYLNGSGVERDAEKCVALLRQAAAQEYPRAVDLLGYCHQHGIGVEKDDAK
                     AVELYTAASDRGFAAAQCNLGLCYENGTGVEMDKARAVELYRRAAEQGHAPSQCNLGF
                     CYLVGIGVEPDP"
BASE COUNT          581 a          894 c         1028 g          482 t
ORIGIN      
        1 gcggataaaa gcggataaaa tttgaaacat gcaggtctcc agcagcttgt cgtcgatctt
       61 gcttgctctt cgcgcggata aaatttgaaa cggcggcaaa aaacgcagaa agaggcttta
      121 gccccgccgc aaaagccgta tgctttcccg caggggggcg tatgctttaa ggccaccgct
      181 gcgttgctct ttcaaaagag ggaaaaaagt gatatagtaa taacgagttg ccgcgcaccg
      241 ggacgcccgt cgcagaagcc ccgattttga ttgccccgcg ataaaaagcg gcttcttccg
      301 gcagtcccgt ttgtcgtcgc tgtgcggcgg ccaaaaccgt ttttatccac ggccttgaac
      361 tctcaggccc ccgcccacaa ggtgggcgga tgcgcataaa agcgcggcga aatcaccctg
      421 cgccggagcc atccggcggc ggaggaggag gacccagtga tcaaaaatat catcttcgat
      481 ctcggcggcg tggtggtgga ttacgacccc aaagcctatc tgtaccggca gttcaacgac
      541 cctgagctgg aggagctgct ctataacgtg atcttcggca gcgaggagtg gaagcagttg
      601 gacgcgggca ccatcacccg cgccctcgca gaacagcaca tgctggccgc cgccggccgc
      661 cgccgctacg aggcgcagct ggtgctggac gactggcgcg agatgatgac caccaggctg
      721 gacacggtgg agctcatcac cgggctcaag gccgcgggat accacatcta ttttttgtcg
      781 aacctgcccg aggacgtcta tcagctgttc accgaaagac gccggttcat gcagctgttt
      841 gagggcggca tccccagttt ttccgtcaag ctgaccaaac cgaaccgcaa gatctttgat
      901 ctgatgctca agacctacgg cctgcggccg gaggagacga tctttgtgga cgacggcaag
      961 gagaatatcg cgacggccca aaagctaggc ctgaccgcga tcccgttcaa agacgccatg
     1021 gatctgcaca agaccctggc ttttctcggc gtggcggtgc cggcaaagct gcgccacgcc
     1081 ccccggccga aagcgccgaa aaaaacaggc cgctggctgc gccgaaaaaa gcgcgccgcg
     1141 gccccggcgg ccgacgcccc gcaggagacg gctcctccgg ccgtcgacct gcccctggag
     1201 gacgacgatg aggacgacga catctgagac cggggccggc gctgtgcacc gacaacagag
     1261 agggtgaaag aaatggagag tcccacgtcc cgcgcaggcg ggcaagagaa tgaaaatcca
     1321 caggatatcg tgtttatgaa taccgagctg atccagcgcg cgctggagga ggtgcgcaag
     1381 tccaaggagg actttgccgc catgctggaa caggccgagc tgggggattc ctcggtgcag
     1441 tacgacctcg ccgtgcgtta tagcgagggc gacggcgtgg gcaaggaccc cgagagagcg
     1501 ttttactggt tcagcgaggc ggcgctgctg ggcgatgtgc gcgccatcgc cgcgctgggc
     1561 cgctgctatc agtttggctc gggggtcgag cgggatctgg aaaaggcgcg ggagcagtac
     1621 gagcttgcgg ccgaccagga ctatgccccc gcgctctgtg atctgggcct gtgctatgaa
     1681 aacggcacgg gggtgccgag agatgaggcg gcggcggcgg agctttatgc ccgcgctgca
     1741 gagcaggaat acgcccccgg cctctgctgt ttgggcctgt gctacgagat gggcaccggc
     1801 gttgagatgg ataagctgcg cgccaccgag ctgtacgcgc agtcggcgga gctggggtat
     1861 gccccggccc agtgcaacct ggggttctgc tatctgaccg gcatcggcgt cccggtggac
     1921 gccgacaagg ccgtgccgct gttccgcaag gcggcggagc aggatttccc gcgggccatc
     1981 gacctgctgg gcaactgcta ttatgagggc gagggggtgg agcaggaccg gcaaaaggcc
     2041 gtcgagtgct atgcccgcgc ggccgggctg gactatgccc cggcccagtg caacctgggc
     2101 ctgtgctatg agctggggca gggggtggag cgggacccgg ccaaggcggc cgcttactac
     2161 atacagtcgg cgcaaacggg ccacgccccg gcccagtgca accttgccgt gtgctacctc
     2221 aacggcatcg gtgtggagcg ggacgaggca aaggccgtga aatggctgga ggccgcggcg
     2281 gcacagcgat tcccccgcgc gttggacctg ctgggcttct gcctgcaaaa cgccgtcggc
     2341 atcgagcggg atgacgagcg cgccgtcctg tgctacaccc gcgcggcgga gcagggctac
     2401 gccccggccc agtgcagcct ggggctgtgc tatgagaacg gcgaggcggt gaatgtggac
     2461 ctcccccgcg cggccgggct gtaccagcag gcggcggacc ggggccacgc gccctcacag
     2521 tgcaacctcg ccgtgtgcta tctcaacggc agcggcgtgg agcgagacgc ggaaaaatgt
     2581 gtggcgctgc tgcggcaggc cgccgcgcag gagtatccgc gggccgtcga cctgctgggc
     2641 tactgccatc agcacggcat cggggtggaa aaggacgacg ccaaggcggt ggagctctac
     2701 acggcggctt cagaccgcgg ttttgccgcg gcccagtgca acctgggcct gtgctatgaa
     2761 aacggcaccg gcgtggagat ggataaggcc agagcggtgg agctgtaccg ccgggcggcg
     2821 gagcagggcc atgcgccctc gcagtgcaac ctgggctttt gctaccttgt gggcatcggc
     2881 gtggaacccg acccttaaaa ggcgatcccc tggtttgaaa aggcggccgg gcaggagttt
     2941 ccgcgcgcgc tggacctgtt gggggattgc tgcggcgagg gccgc
//
LOCUS       DBMDYA010000342         2982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7379_length_2982_cov_1.354391, whole genome shotgun sequence.
ACCESSION   DBMDYA010000342 DBMDYA010000000
VERSION     DBMDYA010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_2982_cov_1.354391"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPFK_08740"
     CDS             complement(<1..213)
                     /locus_tag="ACZPFK_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406438.1"
                     /translation="MNLRQEKRRLARVFAHEIFQARSRLGWTQQKTADTVFISLRWYQ
                     KIEAGRVVPSFLVGICLMKLFDIEPER"
     gene            complement(528..761)
                     /locus_tag="ACZPFK_08745"
     CDS             complement(528..761)
                     /locus_tag="ACZPFK_08745"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1406439.1"
                     /translation="MILYQLFESPTELSLREGKKAFGIQCEKVEDGKAALLQAIGDIS
                     TSRQRVDTLAERCTRAQLSPLHFRDVVDDFLLA"
     gene            complement(739..975)
                     /locus_tag="ACZPFK_08750"
     CDS             complement(739..975)
                     /locus_tag="ACZPFK_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406440.1"
                     /translation="MSINMIFSRVMLNQRISKNLTQSQAAERCGLSLRGYQKLESGRA
                     APTFASALYISNELDFSLDQIKNEVFQDDTVSAL"
     gene            962..1222
                     /locus_tag="ACZPFK_08755"
     CDS             962..1222
                     /locus_tag="ACZPFK_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406441.1"
                     /translation="MFIDMKILLWWGFYVIFPSIGTNSSSHCKAVQIGLGFSLRLGPF
                     YQLFPLPNKAGAPGRHTFPLAGQNAAGGRFAVPAAFAAAEQL"
     gene            complement(1248..2081)
                     /locus_tag="ACZPFK_08760"
     CDS             complement(1248..2081)
                     /locus_tag="ACZPFK_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1406442.1"
                     /translation="MAFYGIDVSHHEGTIDWQRAKVNGPDFCFVRVGYANGDGTITMD
                     REFENNMNGLADTCIDTGLYLYSYIRTEAAAAEAAGTFLTIAQAYDLTMPLVFDLEDV
                     GVYGPLGVELTTSIAQVFLGMVRDAGYYPMLYSNTNFANNYVDMSQLSQFDFWVADYR
                     AWIGYTGDYGIWQYTDQGVIDGIGDRVDLNIAYRDYPSIIKSEGYNRLPAWMRILPGS
                     VVRIRPTAVTYANSSVPIPPCVRRQDYTVLKVCGRTALLSCIHSWVRLEDLQLVQGPA
                     V"
BASE COUNT          580 a          926 c          853 g          623 t
ORIGIN      
        1 ccgctccggc tcaatgtcga agagcttcat caggcaaatg cccaccagaa agctgggaac
       61 cacgcgtccc gcttcgatct tttgatacca gcgcagagaa ataaaaaccg tgtccgcagt
      121 tttttgctgc gtccacccca agcgggagcg ggcctgaaaa atttcatggg caaaaacgcg
      181 cgccagtctc cgtttttcct gccgcaaatt catagggtat tcctcctctg ttttataatg
      241 gaagaatata ccaatttttg cttctaatcc acgaactcgt tgttcatatt atgtaaaatg
      301 gcaaaaaact acatttcgct ttaaaatatt atcctatttt cacaaaaacg gaccatgatc
      361 tgattcagcc gcccctgctt ttctcttctg ttgcggcagc aaaagtcttc tctgcttgtt
      421 tttgcatcca tttatttcat gttttatgat ttaatgcaaa ttgcaaacag caaaggccgg
      481 tcctgcatca ggaccggcct tttatctgcc agtttgcgga tcctttatca cgccaaaagg
      541 aaatcgtcca ctacatcccg aaaatgcaga ggtgacagct gggctcgggt gcaccgctct
      601 gccagggtat ccaccctctg cctgctggtg gagatatccc cgatcgcctg cagcaacgcg
      661 gccttgccgt cctccacctt ttcacattgg atgccgaaag cctttttccc ctcccggaga
      721 gaaagctcgg tcgggctttc aaagagctga tacagtatca tcctgaaaca cctcgttttt
      781 aatctgatcc aagctgaaat ccagttcgtt tgaaatatac agggcgctgg caaaagtcgg
      841 cgcggcgcgc ccgctttcca gcttttgata tcctcgcagt gaaaggccgc atctctctgc
      901 ggcctggctt tgcgtcaggt ttttagagat gcgctgattc agcatcaccc gggaaaaaat
      961 catgtttatc gacatgaaaa tcctcctctg gtggggattt tatgtcattt ttccctctat
     1021 tggaacgaac tccagttcac attgtaaggc tgtacaaatc ggcttgggct tttctttgcg
     1081 gcttggtccc ttttatcagc ttttccccct cccaaacaag gccggcgcgc cggggcggca
     1141 cacttttccc ctggcaggac aaaacgccgc cggcgggcgc tttgccgtgc cggcggcgtt
     1201 tgcggctgca gagcagctgt gaagcgcagc tattttatcg ataacgatca aacggcgggc
     1261 ccctgcacca gctgcagatc ctcgagccgc acccaagagt ggatacagct cagcagcgcg
     1321 gtgcggccgc acactttgag cacagtgtaa tcctgtctgc gcacacaggg cgggatgggc
     1381 acgctgctgt tggcataggt gacggcggtg ggccggatgc gcaccacgct gccgggcaaa
     1441 atgcgcatcc aggccggcag gcggttatag ccctcgctct tgatgatcga cgggtaatcg
     1501 cggtaggcga tgttcaggtc caccctgtcc ccgatgccat caatcacccc ttggtcggtg
     1561 tactgccaaa tgccgtagtc gccggtataa ccgatccagg cgcgatagtc cgccacccag
     1621 aaatcgaact gggacagctg ggacatatcg acgtagttat tcgcaaaatt ggtgttgctg
     1681 tacagcatgg ggtaatagcc cgcgtcccgc accatgccga gaaagacctg tgcgatactg
     1741 gtcgtcagct ccaccccaag agggccatag acccccacgt cctccaggtc gaacaccagc
     1801 ggcatggtga ggtcataggc ctgggcgatg gtgagaaaag tgcccgccgc ctctgccgcc
     1861 gccgcttcgg tgcggatata gctgtaaaga tacagcccgg tatcgatgca ggtgtcggca
     1921 aggccgttca tgttgttttc gaactcccgg tccatggtga tggtgccgtc gccgttggcg
     1981 tagcccaccc gcacaaagca aaagtccggg ccgtttactt tggctctttg ccagtcgatc
     2041 gtcccctcgt gatgtgagac gtcgatccca taaaaggcca tggtcatccc ctctttcttg
     2101 agactttccc catcctatgc cccacgcttt ccgggtgttc cctccccggc ggccgccccg
     2161 cggcccggag ggcatgggca catcttccgc cacggcggca taaagtgtgg cagagcgctt
     2221 tgacggcccg ggctgccgct ctgccctcct gcccggcccc cgcgttccgc tgtaagtttt
     2281 ggccggccgc ttgccggaaa agcggtgtcg cgccccgggg ccgcccaggc tgccgcgcgc
     2341 ggcgaatgga gtttttatgt ccagattttt gtttggagga tgcggcgagg acaactgccc
     2401 agggtgcatg cctccggctc cggatgagcg ggagccccgc tgcggcccgc cggaaaactg
     2461 ttgtcccacc cccgggcccc agggaccggt aggccctcag ggagaacccg gccccgccgg
     2521 gccgatgggc ccacagggcc ccaagggaga aaagggcgac accggctgcc caggtcgccc
     2581 cggctgcccg gggcccgtgg gaccaaaagg ggaccagggc gagcccggct gcccaggccc
     2641 tcagggggaa ccgggttgtc ccggctgccc tggcccgatg gggcccgcag gcccgcgggg
     2701 accccaaggt gaaaagggcg agcccggttg ccccggccct caaggaccga aaggagagcc
     2761 gggctgccca ggctgccccg gtccgatggg gcctataggg cccaaaggag accccggtga
     2821 gcccggctgc cccggtcgcc ctggctgtcc cggtccggtg ggacctcggg gtgagaaagg
     2881 cgagcccggt tgccccggcc ctcaaggacc gaaaggagac ccgggagaac cgggttgtcc
     2941 cggctgcccc ggtccgatgg ggcccgccgg tccacaggga cc
//
LOCUS       DBMDYA010000343         2978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7387_length_2978_cov_1.440042, whole genome shotgun sequence.
ACCESSION   DBMDYA010000343 DBMDYA010000000
VERSION     DBMDYA010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_2978_cov_1.440042"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="ACZPFK_08765"
     CDS             complement(<1..303)
                     /locus_tag="ACZPFK_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406443.1"
                     /translation="MKMESGLYQLVYEYFKARILYGFYGEGDKLPSAPKIGSTFHLAP
                     ATVRVALSALEQNGYIRQEERKTARVAYKTEPGQFRRQAAEYFVPREAGIRDLAESG"
     gene            complement(378..>2978)
                     /locus_tag="ACZPFK_08770"
     CDS             complement(378..>2978)
                     /locus_tag="ACZPFK_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1406444.1"
                     /translation="VPLQLESDWYLISIIPKNVVDAQTTHILTRSLALIVSILAGIAL
                     LAYFYLRYARRANERLRRQADYIGHLYNAVPEGIALIGVTQPYPLLQLNKEGLRMLDY
                     PQENPGEAPLNKSLQDLLFPDDYEALSRVLAVAEKQEKKNTFAVRVHRRGGSLFWAAG
                     IVEKTLDENGVPVLIATFHDVTDQKLAEEEAAREQLQERILLVSAISSVYPVIISMNL
                     SRDTIKFIYLRSGLMVHMGGQTSYTQLYDEIRQSVHPKDRDEFEHRLAPRRLREVLGK
                     EKNEVFVEAKLLLDDGQYHWTSTQIIQVEDPYSADGLAVLISRRIDEQKYEEERQKQA
                     LQSALDNARAASRAKSRFLSNMSHDIRTPMNAILGMTAIAQAHLDDRQRVEECLRKVD
                     LSGKHLLSLINDVLDMSKVESGKLTIKREPFNFAELVTEAAELVRPQAKAHELTLEVR
                     PALLKNERVVGDPLRIRQVLVNVLSNAVKYTPSGGAVYLEFWQEECARKGYQRYGFRC
                     EDTGLGMDKTFLERLFQPFERAADSAVSQVVGTGLGMAITKNLVDLMDGSIEVESEPG
                     KGSVFTIAFPLEVQDAPQDRVPREWLGARSLVVDDDIPVRENVVELLNTIGLRAEPAA
                     DGRDAVRRVLDAQKGPDPFALLIVDWRMPDLDGIEVARRVRAQIGEKVPVVILTANDW
                     AESEAEAKTAGVTAFLSKPFYQSKLCYLLSELSGDTDREKEESKAAGEAFAFPDKRVL
                     LVEDNELNREIARTLIEEHGIQVEEACDGVEAVEKFSLSEPGYYDLILMDVQMPRLDG
                     YGATKKIRALDRPDAERIPIVAMTANAFEEDQRDALQAGMNAHFAKPIDARAFDRLLY
                     GYFSPSSHEN"
BASE COUNT          563 a          961 c          795 g          659 t
ORIGIN      
        1 gcccgactcc gccaggtcgc ggatgccggc ctcgcggggc acaaaatatt cggcggcctg
       61 ccgccggaac tgcccgggct ctgtcttata ggccacgcgg gcagttttgc gctcctcctg
      121 acggatatac ccgttctgct ccagcgcaga gagggccacc cgcaccgtcg caggcgcgag
      181 gtgaaacgtc gagccgattt tgggcgcaga gggaagcttg tcgccctccc cgtaaaagcc
      241 gtaaaggatg cgggccttaa agtactcata aacgagctga tacagcccgc tctccatttt
      301 cattgttcgc aacaccccgg ctcttattct atcatctttt tgcgcccggt gccatccctt
      361 tcctttttca gttttaatca gttttcgtgc gaagacggcg aaaaatatcc gtataacagc
      421 cggtcgaacg cgcgcgcgtc gatgggcttt gcaaaatggg cgttcatgcc cgcctgcagg
      481 gcgtcgcgct gatcctcttc aaaagcgttt gccgtcatgg ccacaatggg gatccgctct
      541 gcgtccgggc gatccagcgc acggatcttt ttggttgccc cataaccgtc caggcgcggc
      601 atctgcacat ccatgagaat caggtcgtaa taccccggct cagacaggga gaatttttcc
      661 accgcctcca cgccgtcgca ggcctcttcc acctggatgc catgctcttc aatcaaggtg
      721 cgcgcgatct cccggttcag ttcgttgtcc tcgacaagca gtaccctctt gtcgggaaag
      781 gcgaatgctt ccccggcggc ttttgattct tccttttctc tgtctgtatc tccactcagc
      841 tcgctcagca gatagcagag cttcgactga taaaagggct tggacaaaaa cgcggtgacg
      901 ccggccgttt tcgcctccgc ctcgctctcc gcccaatcgt ttgccgtaag gatcaccacc
      961 ggcacttttt ctcctatctg ggcccggacg cgccgcgcca cctcgatgcc gtccaggtcc
     1021 ggcatccgcc agtcgacgat cagcagtgcg aacgggtcag ggcctttctg cgcatcgaga
     1081 acacgccgca cagcatcccg tccgtcggcc gccggttcag cccgaagccc gatggtattc
     1141 aaaagctcca caacattttc gcggaccggg atgtcgtcgt ccaccaccag gctgcgggcg
     1201 ccgagccatt cccgcggcac gcgatcctgc ggggcgtcct gtacctccag gggaaaagct
     1261 atggtaaaga cagatccctt gccgggctca ctttccacct cgatactacc gtccatcaga
     1321 tccaccaggt ttttggtgat ggccatgccc aagccggtgc ccacgacctg gctgacagca
     1381 gaatcggccg cccgctcaaa aggctggaac aaccgctcca aaaatgtttt gtccatgccc
     1441 aggccggtgt cctcacagcg aaatccatac cgctgatatc ccttgcgcgc gcattcctcc
     1501 tgccagaact ccagatagac tgcgcccccc gacggggtat attttaccgc gttgctcagc
     1561 acattgacca gcacctggcg gatgcgcagc ggatcgccca ccacgcgctc gtttttcagc
     1621 agggcgggcc ggacctccag ggtgagctcg tgcgctttcg cctgaggcct gaccaattct
     1681 gcggcctcgg tcaccagctc tgcgaagtta aacggctccc gcttgatggt gagcttaccg
     1741 ctctccacct tggacatatc cagcacgtcg ttgatcaggc tgagcagatg cttgcccgag
     1801 agatctacct ttcgcagaca ttcctccacc cgctgccgat catccaaatg tgcctgcgcg
     1861 atggccgtca tgcccaaaat ggcgttcatg ggggtgcgga tgtcgtggct catgttcgat
     1921 aagaatcgac ttttcgccct gctggcggcc cgagcgttat ccaatgcgct ctgcaaggcc
     1981 tgtttttgcc gctcctcttc atatttctgc tcgtcgatgc ggcgggagat cagcacggcc
     2041 agcccatcgg cagagtaggg atcctctacc tggataatct gcgttgacgt ccaatgatac
     2101 tgcccatcgt ccaacagcag ctttgcttcc acaaagacct cgttcttctc tttgcccagc
     2161 acctcccgca gacgccgcgg cgcaaggcga tgttcaaatt catcccggtc tttcgggtgg
     2221 accgactgtc tgatctcgtc atagagctgg gtgtaagagg tctgtccccc catatgcacc
     2281 atcagcccgg agcggaggta gataaacttg atggtatccc tgctgagatt catactgatg
     2341 atgaccggat agacgctgga aatcgcgctc accaacagga tacgttcttg aagctgttcc
     2401 cttgccgcct cttcctccgc cagcttctga tcggtgacat cgtgaaacgt ggcgatcagc
     2461 accggcacgc cgttttcatc cagcgtcttt tcaacgatgc ccgccgccca gaaaaggctg
     2521 ccgccccgcc ggtggaccct caccgcaaac gtattctttt tttcttgctt ttccgcaacc
     2581 gccaagacgc gggacagcgc ctcgtaatcg tccgggaaaa gcaggtcttg caggcttttg
     2641 ttcagcggcg cttccccagg gttctcctgt ggatagtcca gcatccgcag cccttccttg
     2701 ttgagctgca gcagaggata aggctgtgtc accccgatga gggcgatccc ctcgggcacc
     2761 gcgttgtaga gatggccgat gtaatcggcc tgccggcgca gcctctcatt cgcgcgccgg
     2821 gcgtaccgca gataaaaata tgccagcagg gcgatccccg ccaggatgct gacgatcagc
     2881 gccagagagc gcgttaggat atgggtggtc tgggcgtcga ccacattttt ggggatgata
     2941 gagatcaggt accaatcgct ctccagttga agcggcac
//
LOCUS       DBMDYA010000344         2960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7437_length_2960_cov_1.198321, whole genome shotgun sequence.
ACCESSION   DBMDYA010000344 DBMDYA010000000
VERSION     DBMDYA010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7437_length_2960_cov_1.198321"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="ACZPFK_08775"
     CDS             <1..646
                     /locus_tag="ACZPFK_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1406445.1"
                     /translation="AEVRGQLRALGNVNVGAIEEYAEVRERYEFMRGQVADVEKSKAE
                     LTRLIAELSGEMKELFSKSFEQINAHFSRIFAELFGGGSARLSLSDEENVLESGIDIS
                     VSPPGKIIKNLSALSGGEQALVAIAIYFAILAVNPAPFCILDEIEAALDDVNVVRYAS
                     YLRRITAHTQFIVITHRRGTMEEADVLYGVTMQEDGVSKLLKLDVNEVDAKLIS"
     assembly_gap    684..693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            846..>1138
                     /locus_tag="ACZPFK_08780"
     CDS             846..>1138
                     /locus_tag="ACZPFK_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR1406446.1"
                     /translation="MGFFEKLGRGLAKTRDAIFGSIGELENADRIDEDTYEELLEQLI
                     LADTGAEVAEGLVEQLRARVKRDRLKTGAEALTALKELLVEMLTAERPFEPQG"
     assembly_gap    1139..1148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1149..1764
                     /gene="ftsY"
                     /locus_tag="ACZPFK_08785"
     CDS             <1149..1764
                     /gene="ftsY"
                     /locus_tag="ACZPFK_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1406447.1"
                     /translation="RIELRQLVNGVGKTTSIGKLAQLYAGQGKKVLLAAGDTFRAAAA
                     EQLGVWAERAGVPLVRHEEGSDPASVIYDAVSSAIARGCDLVICDTAGRLHNKKSLMD
                     ELSKISRVAAKAGAQGDVETLLVLDAVTGQNAIQQAAQFIDAASATGIILTKLDGTAK
                     GGSVISIKAKLGLPVRFVGVGEGIDDLMPFDPQGFVDALFLKTE"
     gene            1897..2538
                     /gene="rdgB"
                     /locus_tag="ACZPFK_08790"
     CDS             1897..2538
                     /gene="rdgB"
                     /locus_tag="ACZPFK_08790"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1406448.1"
                     /translation="MIFAVASNNQGKIREIQRILDAAGHEAKSQRELGIELEVEETGA
                     TFEANALLKAKALCERSGLPTIADDSGLCVEALNGAPGVYSARYCGRHGDDEANNDKL
                     LAALEGLPRGRRAAKFVSAVCLCMPDGRSLTAMGECPGWVGFERRGTRGFGYDPIFYV
                     GERSYAELSDEEKDGLSHRGKALALLGRALPEFLKDPQGFGPWGAQIVIRDEG"
     gene            2599..2898
                     /locus_tag="ACZPFK_08795"
     CDS             2599..2898
                     /locus_tag="ACZPFK_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR1406449.1"
                     /translation="MLTSKQRALLRAQANGLEVVFQVGKGEIDETLVKTTADCLAARE
                     LIKMKVLETSAYTAREAAEALAAATGSEVVQVIGSRFVLYLQKKKGSRYAPLLAR"
BASE COUNT          565 a          862 c         1067 g          446 t
ORIGIN      
        1 ggcggaggtg cgcgggcagc tccgcgcgct gggcaacgtg aacgtggggg ctattgagga
       61 gtacgccgag gtgcgcgagc ggtacgagtt catgcgcggc caggtggcgg atgtggaaaa
      121 gagcaaagcg gagctgaccc ggctgatcgc cgagctgtcc ggtgagatga aagagctctt
      181 ttcaaagagc ttcgagcaga tcaacgccca tttcagccgc atctttgcag agctgttcgg
      241 cggcggcagc gcccggctct cgctctcgga cgaggaaaac gtgctggaga gcggcatcga
      301 catctcagtg tcgcccccgg gcaagatcat caaaaatctg tcggccctct caggcggcga
      361 gcaggcgctg gtggccattg ccatctattt tgccatcctg gcggtcaacc cggcgccgtt
      421 ctgcatcctg gacgagatcg aggccgcgct ggacgacgtc aacgtggtgc ggtacgccag
      481 ctatctgcgg cgcatcaccg cccacaccca gttcatcgtc atcacccacc gccgcggcac
      541 catggaggaa gccgacgtgc tctacggtgt gacgatgcag gaggacggcg tctccaagct
      601 gctgaagctg gacgtgaacg aggtggacgc gaagctgatc tcgtgagcgc cgcccggccg
      661 ggcaaaaaag gatgccgaga aggnnnnnnn nnnttcaaaa aaatcaggag ctttcggacg
      721 ttgcgcaata cgagcaggag agcgcctgcg cggtctggcg aaaagcgata tgggccgcgg
      781 cgatctgctg cgcggatcaa gcccgctgcg gcagatgcgg gatcaacttt ttgtgaggtg
      841 cagatatggg cttttttgaa aaactgggcc gcgggctggc caagacccgc gacgccatct
      901 ttggttccat cggcgaactg gagaatgccg accgcatcga cgaggatacc tatgaagagc
      961 tgctggaaca gctgatcctg gccgacaccg gcgcggaggt ggcggagggg ctggtggagc
     1021 agctgagggc gcgggtgaag cgcgaccgcc tcaagaccgg ggccgaggcg ctgacggcgc
     1081 tgaaagagct gctggtggag atgttgaccg ccgagcgtcc ctttgagccg cagggcaann
     1141 nnnnnnnncc gcatagagct gcgccagctt gtcaacggcg tgggcaagac cacctccatc
     1201 ggcaagctgg cgcagctcta tgcgggccag ggcaaaaagg tgctgctggc ggcgggggac
     1261 actttccgcg cggcggcggc cgagcagctg ggcgtctggg ccgagcgggc aggggtgccc
     1321 ttggtgcggc atgaggaggg ctcggacccg gcgtcggtga tctacgacgc ggtctcctcc
     1381 gccatcgccc gcggctgcga tctggtgatc tgcgacaccg ccgggcggct gcacaacaaa
     1441 aagagcctga tggacgagct ttccaagatc tcccgcgtgg ccgccaaggc cggggctcag
     1501 ggggacgtgg agacgctgct ggtgctggac gcggtcacgg ggcagaacgc catccagcag
     1561 gcggcgcagt tcatcgacgc tgccagcgcc accggcatca tcctcaccaa gctggacggc
     1621 acggccaagg gcggctcggt catctcgatc aaggcaaagc tgggtctgcc ggtgcggttc
     1681 gtcggcgtgg gcgagggcat cgacgacctg atgccctttg acccgcaggg ctttgtggac
     1741 gcgctgttcc tgaaaacgga atgagccggc cggcccgcgg ggagcgcggc cccagcccat
     1801 ggggcaaaag cggccgccgc ggggcgcata cagaaaatcc taagaaaatc acaacagaac
     1861 cgtcaaatat ttttgataag atcagcgagg tgcagcatga tttttgccgt tgcgagcaac
     1921 aaccagggta agatccggga gatccagcgc attttggacg ccgccgggca tgaggcgaag
     1981 agccagcggg aactgggcat cgagcttgag gtggaggaga ccggcgccac ctttgaggcc
     2041 aacgcgctgc tgaaagcgaa agcgctctgc gagcggtccg gcctgcccac catcgccgac
     2101 gactccggcc tgtgcgtgga ggcactgaac ggggcgccgg gggtctactc agcccgctac
     2161 tgcggccgcc acggggacga cgaggccaac aacgacaagc tgctggccgc attggagggc
     2221 ctgccgcgcg gacggcgggc ggcaaagttc gtcagcgcgg tctgcctgtg catgccggac
     2281 ggccgcagcc tcaccgccat gggcgagtgc ccgggctggg tgggctttga gcgccggggg
     2341 acgaggggct tcggctatga ccccattttt tacgtgggcg agcgcagcta tgccgagctg
     2401 tcggatgagg aaaaggacgg cctctcccac cggggcaagg cgctggcgct gttgggccgg
     2461 gcactgccgg agtttttgaa agacccgcag ggattcgggc cctggggcgc gcagatcgtc
     2521 atccgggacg agggttgagg tcccgcgctc cgatgcgggc gtaaggggcc gctacacaag
     2581 acaggtaaag gagaaattat gctgacaagc aaacagcgcg cgctgctgcg cgcacaggcc
     2641 aacggcctgg aggtcgtgtt ccaggtgggc aagggggaga tcgacgagac tttggtcaag
     2701 accaccgccg actgtctcgc cgcgcgggaa ctgatcaaga tgaaagtgct ggagaccagc
     2761 gcctataccg cccgggaggc ggccgaggcg ctggccgcgg ccaccggcag cgaggtggtg
     2821 caggtcatcg gctcccgttt cgtgctctac ctgcagaaaa agaaaggctc cagatacgcg
     2881 ccgctgctgg cgagatagtg gtgccggcgg gcggggcgcg cgcccggcag gacgggcgga
     2941 acgcgcggcg cccgcgaggg
//
LOCUS       DBMDYA010000345         2958 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7446_length_2958_cov_1.212461, whole genome shotgun sequence.
ACCESSION   DBMDYA010000345 DBMDYA010000000
VERSION     DBMDYA010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7446_length_2958_cov_1.212461"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="ACZPFK_08800"
     CDS             <1..265
                     /locus_tag="ACZPFK_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HIR1406450.1"
                     /translation="GKWLLNDPEILFVDEPTKGIDVGTKADFYTLMSDMAARGVSIVM
                     VSSDMPELISMSDRCLVLNEGRITAELKKGEITETAVMKAAIS"
     gene            309..1235
                     /locus_tag="ACZPFK_08805"
     CDS             309..1235
                     /locus_tag="ACZPFK_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1406451.1"
                     /translation="MAEKKAPTSGPGFSKPVKKIDVHTHLAKEENAMGMFTAEERIEF
                     DKVMGIDKCVILPFAGRMPVSAGTQAPPSTGLLTPEDACEVAARHPDHFAWFCNVFPE
                     GTDATYAQLKKYKEMGACGVGEFGSLQRFDDPMMDHLFSCCEELGLPLLFHMSPNGIN
                     YGVIDEAGLPLLEKALIRHPKMAFIGHSQPFWFEISTYPENLTPEERNIYPGGRVTPG
                     RVPYLLDKYPNLYADLSADSGGNALMRDPEYGIEFLNRYQDKLMFGTDICNTEFIYPL
                     GSYLDSLLHQLKISEEVYSKVCRLNAQKLLGL"
     gene            1469..2857
                     /gene="uxaC"
                     /locus_tag="ACZPFK_08810"
     CDS             1469..2857
                     /gene="uxaC"
                     /locus_tag="ACZPFK_08810"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR1406452.1"
                     /translation="MSFMDAGFLLSGETARKLYDQTAGMPIFDYHNHLNPQEIVENKN
                     YGSVTSMWLARDHYKWRILRAAGAPERCICGEADDREKFRAFAAACEKAIGSPVYYWT
                     NLELQRLFGAEEPLTRQSADRLYELCNRRLQQEDLRPRELLRKLKVRVLCTTEAPDSA
                     LEWHRAFARRADGGLRMSPGFRPDGVLHIGKPGWTDSVKKLLAAEEVGEESLDGLEAA
                     LAAALGRFKELGCFTSDHGFERFAYRPAGKEEARAAFRQALRGETLTQEQQDGYVGYL
                     LGALAALYHSRGIVMQLHLGALRDANTRGFARLGPDGGYDSVGRATDPFALAAFLDAL
                     AQRDQLPELILYCLNPCDYPVLATVCATFGGGRPGRVQLGAAWWFNDTERGIREQLQY
                     LFENGMFAYAPGMLTDSRSIGSFVRHEYYRRIVCDEVGALLDSGRYHSFDTAQGMLED
                     VFYNNAERLFIR"
BASE COUNT          557 a          908 c          993 g          500 t
ORIGIN      
        1 gggcaaatgg ctgctcaacg accccgaaat cctttttgtg gacgagccca ccaagggcat
       61 cgacgtgggc accaaagcgg atttctacac cctgatgagc gacatggccg cccgcggcgt
      121 atcgatcgtg atggtgagct cggatatgcc cgagctgatc tcgatgagcg accgctgcct
      181 ggtgctcaac gaggggcgca tcacagccga gctgaaaaag ggcgagatca ccgagaccgc
      241 ggtcatgaaa gccgcgatct cctgaggacg gtttcgatac ggcacaaacg acatgggagg
      301 gaaacgatat ggcggaaaag aaagcgccca cctcgggccc gggtttttca aaacccgtca
      361 aaaagatcga cgtgcatacc caccttgcaa aagaagagaa cgccatgggc atgttcaccg
      421 cggaggagcg gatcgagttc gacaaggtca tggggatcga caagtgcgtg atcctgccct
      481 ttgcgggccg gatgccggtc tcggcgggca cccaggcgcc gcccagcacc ggcctgctca
      541 cgccggagga cgcctgcgag gttgcggccc gtcacccgga tcatttcgcc tggttctgca
      601 acgtcttccc cgaggggacg gatgccacct atgcccagct caagaaatat aaggagatgg
      661 gcgcctgcgg cgtgggcgag ttcggcagcc tgcagcggtt cgacgacccc atgatggatc
      721 acctgttcag ctgctgcgag gagctgggcc tgccgctgtt gttccacatg agccccaacg
      781 gcatcaacta cggcgtcatc gacgaggcgg gcctgccgct gcttgaaaaa gcgctgatcc
      841 gtcatcccaa gatggcgttc atcggccatt cgcagccgtt ttggtttgag atctccacct
      901 atcccgaaaa cctcacgccg gaggagcgga acatctaccc cggcggcagg gtgacgccgg
      961 gccgggtgcc ctatctgctg gacaagtacc ccaacctcta cgcggacctc tcggccgaca
     1021 gcggcggcaa cgcgctgatg cgagatcccg aatacggcat cgagttcctg aaccgctacc
     1081 aggacaagct gatgttcggc accgacatct gcaacacaga gttcatctat ccgctgggct
     1141 cttatctgga ttcgctgctg caccagctca agatcagtga agaagtctac tccaaggtct
     1201 gccgtctcaa cgcgcagaag ctgctgggcc tgtagtacag gggacccgcg gctgcaggcg
     1261 gggggaacgg cgcggcccgc gccgggccct gccggccatt ccggcgggcg gtagagctgc
     1321 cgtccgccgg gctggcggcc cccttttgca gcctgcgcgg cgcccgccgc cccgtgtgcg
     1381 agagggggcc gctttgctgt cttggcaaag ccgccgcccg ccggcggagc gcgcgcgggg
     1441 caatcggatg aggaaggatg ttaaagagat gagttttatg gatgcggggt tccttctctc
     1501 gggggaaacg gcccgaaaac tgtatgacca gaccgccggg atgccgatct tcgattacca
     1561 caaccaccta aacccgcagg agatcgtcga aaacaagaac tatggctccg tcacctccat
     1621 gtggctggcg cgggaccatt acaagtggcg catcctgcgc gccgcgggcg cgccggagcg
     1681 ctgtatctgc ggcgaggcgg acgaccggga gaagttccgc gcttttgccg ccgcctgcga
     1741 aaaggcgatc ggcagcccgg tctactactg gacgaattta gagctgcaaa ggctgttcgg
     1801 cgcggaggag ccgctgaccc ggcagagcgc cgaccgcctg tatgagctgt gcaaccgcag
     1861 gctgcagcag gaggacctgc gcccgcggga gctgctgcgc aagctgaaag tgcgggtgct
     1921 ctgcaccacc gaagcccccg actcggcgct ggagtggcac cgggcctttg cgcggagggc
     1981 ggacggcggc ctgcgcatgt cgccgggctt tcggccggac ggcgtgctgc atatcggcaa
     2041 gccgggctgg acggacagcg tcaaaaaact gctggcggcc gaggaggtgg gggaggagag
     2101 cctggacggc ctggaggcgg cgctggccgc cgcgctgggc cgcttcaagg agctgggctg
     2161 ctttaccagc gaccacggct ttgagcgctt cgcctaccgg ccggccggta aagaggaggc
     2221 gcgggccgct ttccggcagg cgctgcgggg cgaaacgctg acgcaggagc agcaggacgg
     2281 ctatgtcggc tatctgctcg gcgcgctggc ggccctttac cacagccgcg gcattgtgat
     2341 gcagctgcac ctgggcgcgc tgcgggacgc caacacccgc ggctttgcac gcctggggcc
     2401 ggacggcggt tacgacagcg tggggcgcgc caccgacccg tttgcgctgg cggcctttct
     2461 ggatgcgctg gcgcagcggg accagctgcc ggagctgatc ctctattgcc tcaacccctg
     2521 cgattacccg gttctggcca ccgtgtgcgc cacctttggc ggcggccgcc cgggccgggt
     2581 gcagctgggc gccgcgtggt ggttcaacga caccgagcgc ggcatccggg aacagctgca
     2641 atacctgttt gaaaacggca tgttcgccta tgcgccgggc atgctgaccg actcgcgcag
     2701 catcggctct tttgtgcggc acgagtatta ccggcgcatc gtctgcgacg aggtgggggc
     2761 cttgctggac agcggcaggt atcacagctt cgacacggcg caggggatgc tggaggatgt
     2821 cttctacaac aatgccgagc ggctttttat caggtgattt tttcacttaa aacggttctt
     2881 gaccgacagg atgaatagga ggatgaggat atgaaactca gggagaactg caagtatgac
     2941 ctgatgacgg tcaccagc
//
LOCUS       DBMDYA010000346         2957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7447_length_2957_cov_1.711835, whole genome shotgun sequence.
ACCESSION   DBMDYA010000346 DBMDYA010000000
VERSION     DBMDYA010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_2957_cov_1.711835"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="ACZPFK_08815"
     CDS             complement(<1..585)
                     /locus_tag="ACZPFK_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1406453.1"
                     /translation="MTGYEHCTLCPRRCGVDRTRTAGPCGGTAELRLARAALHFWEEP
                     CISGEGPNAPGSGTVFFSGCPLRCCFCQNFEISEQNRGKAVTEQRLAEIFLELQARGA
                     ANLNLVTGTHYLPGILRALSLARPALALPVAWNCGGYETLESVEALAGEVNIFMPDLK
                     YQDPALSGRFSHAPDYFEAASRAVCRMVELAGPPV"
     gene            847..1860
                     /locus_tag="ACZPFK_08820"
     CDS             847..1860
                     /locus_tag="ACZPFK_08820"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR1406454.1"
                     /translation="MHSEMILFETQQRPEGYRIFGTETGAYTAYHSLSPDGFSPDNSV
                     AGFTRELLAPDLYPGRRSYFHILDEAGNHFVTAPRGLGFESVLNFRELGGYPAGDGRR
                     VRWGRFYRSARLGTISPEEKQRFAQLGIRVVLDLRSDSEIAQMPDPVFEGSEQKVISA
                     LFQKNKQQVNLDPGVLFTFSPEEMLKDDAEFTERYRQMPFDNEAYQYLFDKLEKGEAP
                     VLFHCTAGKDRTGVAAMLILLSLGVSWETIKEDYLLTNLFCRDIVDKVLDKYYKPGQP
                     EHVRHFLTCIAGVELRNLEAAYEALLDRYGDVEHYFSAEFGLQGPRLERLKDRYLEEV
                     KPR"
     gene            1857..2744
                     /locus_tag="ACZPFK_08825"
     CDS             1857..2744
                     /locus_tag="ACZPFK_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1406455.1"
                     /translation="MKLLSFGSLNLDQVYRVRQFARPGESVRALETQTFCGGKGLNLS
                     IAAAAAGCEVWHAGKVGPDGAVLIERLIRSGVDTTLISTGGTVSGHAIIQVNDEGENK
                     ILIAPGANGEITPQECEAAVRRFSAGDWLALQNETSCLPELLRLGKEQGMTIALIPSP
                     VDGELEAADLSAVSYFILSRYDGSALTGKQAPDEICGELLRRCPEAHVVLNMGAAGSL
                     YCTARQTLHQHAYPSQVVDPTGAGDIFAGYFIARMAAGEKPERALRSAAKAAALSTAH
                     MGACEYVPTPQEVENAYRL"
     gene            complement(2732..>2957)
                     /locus_tag="ACZPFK_08830"
     CDS             complement(2732..>2957)
                     /locus_tag="ACZPFK_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406456.1"
                     /translation="GADDLSFTERQIVEAYRGQPEMQTAVQRVLDIHKSSKKQKCSGH
                     PGAFCFSLAGPAGGPKKQPPRGFFKTAQSR"
BASE COUNT          537 a          933 c          988 g          499 t
ORIGIN      
        1 caccggcggg ccggctagct ccaccatccg gcatacggcc cggctggccg cttcaaaata
       61 atccggcgcg tgggaaaaac ggcccgacag cgcggggtcc tgatatttga ggtcgggcat
      121 gaaaatattc acctcaccgg ccagggcctc cacactctcc agcgtctcgt agccgccgca
      181 gttccaggcc accggcagcg ccagggccgg gcgggccagg gacagcgccc gcaggatgcc
      241 gggcaggtaa tgggtgccgg tgaccaggtt gaggttggcc gcgccgcggg cctgcagctc
      301 taaaaagatt tcggccagcc gctgctcggt caccgccttg ccgcggtttt gctcgctgat
      361 ctcaaagttc tggcaaaagc agcagcgcag cgggcagccg ctgaaaaaca cggttccgct
      421 gcccggcgcg tttggcccct cgccgctgat gcagggctcc tcccaaaaat ggagtgcggc
      481 ccgcgccagg cgcagctcgg ccgttccgcc gcaggggccg gcggtgcggg tgcggtccac
      541 gccgcagcgg cgcgggcaaa gggtacagtg ctcatatcct gtcaagagat gccatgcctc
      601 ccgtaaaagt actgcaaata gtgtaccata tcccccgcgg gatgtggtat aatagccgca
      661 aagtagtctg aaaaaaagcg gtcggccccg cctttgcgtg ggcggccgcc ggggaggcgc
      721 agggcgctgt ccgcgccgcg cgcctccggc accatcataa cataaataga acgtcagagc
      781 aaaaactccc tctgctggcc gcgccccttt cgcgggccgg ggagggaggc gggacaggag
      841 tcaacgatgc acagtgaaat gattttgttc gagacgcagc aaaggccgga gggctatcgg
      901 atcttcggca cagagacggg ggcttacacc gcctatcaca gcctttcccc cgacggcttt
      961 tcgccggaca acagcgttgc cgggttcacc cgggagctgc tcgcgcccga cctctatccg
     1021 gggcggcgca gctatttcca catcttggac gaggcgggca accactttgt caccgctccc
     1081 cgcgggctgg gctttgagag cgtgctcaat ttccgtgagc tcggcggcta cccggccggc
     1141 gacgggcgca gggtgcgctg gggcaggttt taccgcagcg cgcggctggg aaccatcagc
     1201 ccggaggaga aacagcgctt tgcacagctg ggcatccggg tcgtgctgga cctgcgcagc
     1261 gacagcgaga tcgcccagat gccggacccg gtgttcgagg gcagcgagca aaaggtgatc
     1321 tcagccctgt ttcaaaagaa caaacagcag gtgaacctgg accccggcgt gctgttcacc
     1381 ttttcgccgg aggagatgct caaggacgac gccgagttca ccgagcgcta ccggcagatg
     1441 ccctttgaca acgaggccta ccaatacctg ttcgacaagc tggaaaaggg cgaggcgccg
     1501 gtgctctttc actgcacggc gggcaaggac cgcaccggtg tggccgccat gctgatcctg
     1561 ctgtcgctgg gcgtgtcttg ggagaccatc aaggaggatt acctgctgac caaccttttc
     1621 tgccgggaca tcgtggacaa ggtgctagac aagtattaca agcccggcca gccggagcat
     1681 gtgcggcact tcctcacctg tatcgcgggg gtggagctgc gcaacctgga ggcggcgtac
     1741 gaggcgctgc tggacagata cggcgatgtg gagcactatt tcagcgccga gttcgggctg
     1801 cagggcccgc gcctggagcg gctgaaagac cggtatctgg aggaggtgaa gccgcgttga
     1861 agctgctctc tttcggctcg ctcaacctgg accaggtcta ccgggtgcgc cagttcgccc
     1921 gcccgggcga gtcggtgcgc gcgctggaaa cccagacctt ttgcggcggc aagggcctca
     1981 acctgagcat cgccgccgct gcggccgggt gtgaggtctg gcacgcgggc aaggtggggc
     2041 cggacggcgc cgtgctcatt gagcggctca tccgctccgg ggtggatacc acccttatct
     2101 ccaccggcgg cacggtgagc gggcacgcca tcatccaggt caacgacgag ggtgagaaca
     2161 agatcctcat cgccccgggc gccaacggcg agatcacccc acaggaatgc gaggcggcgg
     2221 tgcggcgctt ttccgccggc gactggctgg cgctgcaaaa cgagaccagc tgcctgcccg
     2281 agctgctgcg cctgggcaaa gaacagggca tgacgattgc gctgatcccc tcgcctgtgg
     2341 acggtgaact ggaggcggcg gacctgtctg cggtttccta tttcatcctg agccgctatg
     2401 acggcagcgc gctgaccggc aagcaggccc cggatgaaat ttgcggcgag ctgctgcggc
     2461 gctgccccga ggcacatgtg gtgctgaaca tgggggcggc gggcagcctt tactgcaccg
     2521 cccggcagac gctgcaccag cacgcctatc cctcgcaggt ggtggacccc accggcgcgg
     2581 gggacatctt tgcgggctat ttcatcgcgc gcatggcggc gggcgaaaag ccggagcgcg
     2641 ccctgcgcag cgctgcaaaa gcggccgcgc tgtccaccgc ccacatgggc gcctgtgagt
     2701 atgtgcccac cccgcaggag gtggaaaacg cttatcgact ttgagcggtc ttgaaaaagc
     2761 cgcgcggcgg ctgctttttt ggtcctccgg ctgggccggc gagagaaaaa caaaatgctc
     2821 cgggatgtcc ggagcatttc tgcttttttg aacttttatg gatatccaag acccgctgca
     2881 cggccgtctg catctccggc tgtccgcggt aggcttccac gatctggcgc tcggtaaagc
     2941 tcaggtcgtc ggcgccg
//
LOCUS       DBMDYA010000347         2955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7454_length_2955_cov_1.171689, whole genome shotgun sequence.
ACCESSION   DBMDYA010000347 DBMDYA010000000
VERSION     DBMDYA010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7454_length_2955_cov_1.171689"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1830
                     /locus_tag="ACZPFK_08835"
     CDS             136..1830
                     /locus_tag="ACZPFK_08835"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1406457.1"
                     /translation="MSYDANGGSGDQLPGAVETTAHAPVEVDFSVQPGRPGYQFLGWD
                     EDPLSSNARYPAGGNASLTMPEGGLTLYAVWKIDEAQTATAAYTVHYYVDGALQPDWS
                     ESYSKTTLVADDPAPAYVVDAVTDHLADLGADYYTASRPDLPATLSGEGNVLEVRYAH
                     KLVDVAGTGIELSHNYVYTRVIDGETSVNNFSRVAGGFAAQPGSLLRLQDLIQTVDPE
                     GNTAYEPTGAAVTRTRLPLVEPETETAPVQQDAARQALSDAEAALAQAQAAREEAQQN
                     KTAADEALAAAQAGLTAAQAAQAGVDAAAVEAARQQLQELAAREGALSEEELALKAQL
                     QGIVDAGAAADTAAAEAQALADQRSGEAQAATDALAAAESRLAEAQQALSGAQQALAA
                     AQSAVTASAAAATLAVGESETVALNADGTFLYEAGYTYQVRLDYAKTYVYNTPVEPEE
                     EIVSPEPNPPVVPAPVLPVLPQEEPAAQTVTPAVVTPPAAGTVAISDDDVPLGSMDLR
                     GKSRVRRGSYVILDESVPLGSLPKTSGSMDASWGALGAFLLAAGAALGLKGKRKEQ"
     gene            2194..>2955
                     /locus_tag="ACZPFK_08840"
     CDS             2194..>2955
                     /locus_tag="ACZPFK_08840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1406458.1"
                     /translation="MTVTQEEKFALAAPDRMACAQKLTDLLSGAQTKSSAVFKLALVD
                     FRSFNDIFGRHYGDLLLEQVNAYLAGFEGAEVLRSGGVEFLLILRGSGYARAGEIGEE
                     ICDRFNETWRIGDMDFVCAMSLGIVLPPLAAEKGEALLEMLDSAEHEAYLSGQNTAVT
                     YTEELRENLLRTKAIATRLKNALAVADDPDLEVCYRPTVLYDAHRYTRAESYVRLFSD
                     TYGLVSEATLIPIAEQSGLACALNLYAIRHTCALIR"
BASE COUNT          536 a          974 c          995 g          450 t
ORIGIN      
        1 ccgacgccga agccgacggc aaccccgacg ccgaagccga cagcaacccc gacgccgaag
       61 ccgacagcaa ccccgacgcc gaagccgacg gcaaccccga cgccgaagcc gacgccggaa
      121 ccggcaaaat accttttgtc ctatgacgcc aacggcggca gcggggacca gcttcccggc
      181 gccgtcgaga cgacggccca tgcccctgtg gaggtggatt tttccgtcca gcccggccgt
      241 cccggctatc agttcctcgg ctgggatgag gacccgctct ccagcaatgc ccgttatccc
      301 gctgggggca atgcctcttt gaccatgccc gagggcggtc tgacccttta tgcggtgtgg
      361 aagatcgacg aggcccagac cgccacggcg gcctataccg tgcactacta tgtggacggc
      421 gctctgcagc cggattggag cgagagctat tccaagacga cgctggtcgc ggatgacccc
      481 gcgcccgctt atgtggtgga cgcagtcacc gaccatctgg ccgatctggg agcggactac
      541 tacaccgcct cccgtcctga tctgccggcc acgctcagcg gtgagggcaa tgtgctggag
      601 gtgcgctacg cgcacaagct ggtcgacgtg gccggcaccg gtatcgagct ctcccacaat
      661 tatgtctaca cgcgggtcat cgacggcgag acgagtgtga acaatttctc ccgcgtcgcc
      721 ggcggcttcg cggcccagcc gggttcgctg ctgcgcttgc aggacctgat acagacggtg
      781 gaccccgagg gcaacaccgc ttatgagccc accggggcgg cggtcacccg cacccgcctg
      841 ccgttggtag agcctgagac ggaaacggcc cctgtgcaac aggacgccgc ccgacaggcc
      901 ctcagcgacg cagaggccgc gctggcacag gcgcaggccg cccgggagga ggcccagcag
      961 aacaaaaccg cggccgacga ggcccttgcc gctgcgcagg ccggtctcac cgctgcacag
     1021 gcggcccagg cgggcgttga cgccgcggcg gttgaggccg cccggcagca gctgcaagag
     1081 cttgcggccc gcgagggcgc gctcagtgaa gaggagttgg ccctgaaagc gcaacttcaa
     1141 ggcatcgtgg atgccggcgc ggcggcggat accgcggccg cggaggcgca ggcgctggca
     1201 gaccagcgca gcggcgaggc ccaggcggcg acggatgcac tggctgccgc cgagagcaga
     1261 ctggccgagg cgcagcaggc gttgtccggg gcccagcagg ccctggcggc cgcgcagagc
     1321 gccgtgaccg cctccgctgc ggctgccaca ctggccgtgg gcgagagcga gacggtcgcg
     1381 ctgaacgcgg acggcacttt cctctacgag gcgggctaca cctatcaggt gcgtctcgac
     1441 tacgccaaga cctatgtcta taacaccccg gtcgagccgg aagaggagat cgtttccccc
     1501 gagccgaatc ccccggtggt gcccgcgccg gtgctccccg tgctcccgca ggaggaaccg
     1561 gctgctcaga ccgtcacccc cgcggtcgtg acgccgccgg cagcgggtac ggtggccatc
     1621 tctgacgatg acgtgcccct ggggtccatg gacctgcgcg gcaaaagccg tgtgcgccgt
     1681 ggcagctatg tgattttgga tgaatcggtg cctctggggt cgcttcccaa aaccagcggc
     1741 tcgatggacg cctcctgggg cgcgctgggc gcgtttctgc tggcggcggg cgccgcgctg
     1801 ggcctgaaag gcaagcgcaa ggagcaataa aaaacagctg cgggctgttt ttagccctcc
     1861 atttcggctg ccacagccga cggcgggagg caaaggcccc cggagcaagc cgttttttcg
     1921 gcgctccggc gggcctttcg ccgcggccaa gcgaaatatg ttgtttttgc gggcatgttt
     1981 tttgcacgcc tgttgcaaaa cacgttgaga atccgcggcg aaaaagctat aataaagtac
     2041 aaggatggcc gcgcggtagg gttagcactc cgcgcggcca agccggtttg gagccgggcc
     2101 gggcgcagag gatgccgggc ggccccaaaa ccggcatccc gggggatcaa aaaaagccga
     2161 cggcgtcaag gcgtcggcca gggagggaca gcaatgaccg tcacacagga agaaaagttc
     2221 gccctagcgg cgcccgaccg catggcctgc gcgcagaagc tcacagacct gctcagcggg
     2281 gcacaaacga agagctccgc cgttttcaag ctggcgctgg tggatttcag aagctttaac
     2341 gatatcttcg gccggcatta cggcgacctg ctgctggagc aggtgaacgc ctatctcgcc
     2401 ggctttgagg gcgcggaggt gctgcgcagc ggcggggtgg agtttttgct gatcctgcgc
     2461 ggcagcggct atgcgcgggc gggcgagatc ggcgaggaga tctgcgaccg gtttaacgag
     2521 acctggcgca tcggggacat ggattttgtg tgcgccatgt ctctgggcat cgttctgccg
     2581 cccttggcgg ctgaaaaagg ggaagcgctg ctggaaatgc tggacagcgc cgagcacgag
     2641 gcctacctca gcgggcagaa taccgccgtg acctataccg aggagctgcg cgagaacctg
     2701 ctgcgcacca aagccatcgc cacccgcctg aaaaatgcgc tggccgtggc cgacgacccc
     2761 gatctggagg tctgctaccg ccccaccgtg ctctatgacg cgcaccgcta tacccgggcg
     2821 gaaagctatg tgcggctttt ttccgacacc tacgggctgg tcagcgaggc cacgctgatc
     2881 cccatcgccg agcagagcgg cctggcctgc gcgctgaacc tctatgcgat ccgtcacacc
     2941 tgcgccttga tccgc
//
LOCUS       DBMDYA010000348         2950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7477_length_2950_cov_1.436293, whole genome shotgun sequence.
ACCESSION   DBMDYA010000348 DBMDYA010000000
VERSION     DBMDYA010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7477_length_2950_cov_1.436293"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="ACZPFK_08845"
     CDS             <1..267
                     /locus_tag="ACZPFK_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR1406459.1"
                     /translation="YLHGAPAEHPWASPLRAPSLRGLPPAVFAAAECDVLVDDGLEYA
                     QRLQEAGTAVEYHLYEGMPHGFLVHPYRQTFAALDAVGRFCAGI"
     regulatory      338..421
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            598..1674
                     /gene="gcvT"
                     /locus_tag="ACZPFK_08850"
     CDS             598..1674
                     /gene="gcvT"
                     /locus_tag="ACZPFK_08850"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR1406460.1"
                     /translation="MEKKTPLYEKHVALGGKMVPFAGYLLPVQYQSGVIAEHMAVRGH
                     AGLFDVSHMGEALLTGPDAEKNLDHILSNSFAGMPAGAARYALMLYENGGVVDDLIVY
                     RLGGQQFFLVLNAANTEKDLAWLRGHLSGDAALEDLSPRVAQIALQGPEAKALISTVA
                     DAAQLPQKYYTFTREMQVAGARCLVSRTGYTGEMGYELYLDPADASGVWDALVAAGAT
                     PCGLGARDTLRLEAAMPLYGHELSATIDPLTAGLGFAVKLEKPDFIGKGALLAVGEPK
                     RVRAGLKVTGRGVLREEQEVYRDGKKVGVTTSGTHCPAVGVSCAMAYLDRAAAAVGTV
                     VEVDVRGRRVAAEVVPLPFYKKSK"
     gene            1754..2140
                     /gene="gcvH"
                     /locus_tag="ACZPFK_08855"
     CDS             1754..2140
                     /gene="gcvH"
                     /locus_tag="ACZPFK_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR1406461.1"
                     /translation="MNFPENLLYSKSHEWVEKTGETTARVGLTDYAQDALGDLVFVNL
                     PEPGDAVTDGEPFADVESVKAVSDVYSPVTGAVAAVNEELLDRPQLINESPYEAWFVE
                     VEEIAAFGELMDAAAYEAYCKAEQEK"
     gene            2177..>2950
                     /locus_tag="ACZPFK_08860"
     CDS             2177..>2950
                     /locus_tag="ACZPFK_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR1406462.1"
                     /translation="MGSYIPSTGAQREEMLRAVGAPSFEALYADVPQEIRFPALQMGE
                     GRSELEVSAELRAMAAKNHVFSSIFRGAGAYDHYIPSIVKRVTAKEEFVTAYTPYQAE
                     ISQGVLQSIFEYQTMICELTGMAAANASVYDGATAAAEAAAMCRDRRHSRVLVSAAAH
                     PAVRSTVKTYCEGAGVPMVEVPAREGRTDLEALKEMLAAGDACFYLQQPNFYGVLEDA
                     AAFGEAVHAAGAKFIMGVNPIAAAALATPAECGADIAVGE"
BASE COUNT          530 a          867 c         1071 g          482 t
ORIGIN      
        1 tatctgcacg gggcgccggc ggaacacccg tgggcctcgc cgctgcgcgc cccgtccctg
       61 cgcgggctgc ccccggccgt ctttgcggcg gcggaatgcg acgtcctggt ggacgacggg
      121 ctggagtacg cccagcggct gcaggaggcc ggcacggcgg tggaatacca tctgtacgag
      181 ggcatgccgc acggctttct ggtgcacccc taccgccaga cctttgcggc gctggacgcc
      241 gtggggcggt tttgcgcggg gatttgactt ttggggaaat ctttcttgac ttagccgcat
      301 aaagttttta tactgagagt gaaaagtgaa tcactggatg aagctgccgg gagagaccgc
      361 gttatgcggc gccgaagggg aaacgccgaa aactctcagg caaagggacc ggcagcggac
      421 aaaactccga aaagccagat ggatggcacc gaagaagcaa tccccgccgc gggcggggag
      481 aatctttcag gttggatgac ggggcgctca ggatgtctgc gcgccccgtt tttattgtta
      541 atccaatgtc aatgtcgcgg cccgcagcgg ccgcggcaca aaaaaggagg gatcggtttg
      601 gagaaaaaga cgcctctgta cgaaaaacat gtggccctgg gcggcaagat ggtgcccttt
      661 gcgggctatc tgctgccggt gcagtaccag agcggggtca tcgccgagca catggcggtg
      721 cgcgggcacg ccggcctgtt cgacgtctcc cacatggggg aggccctgtt gaccgggccg
      781 gatgcggaga aaaacctgga ccatatcttg tccaacagct ttgccggtat gccggcgggg
      841 gccgcgcggt acgcgctgat gctgtatgaa aacggcggtg tggtggacga cctgatcgtc
      901 tatcggctgg gcgggcagca attctttctg gtgctcaacg cggccaacac cgaaaaagac
      961 ctggcctggc tgcgcggcca cctcagcggg gacgcggcgc tggaggacct gtccccccgg
     1021 gtggcccaga tcgccctgca ggggccggag gcaaaggccc tgatctccac ggtggcggac
     1081 gcggcgcagc tgccgcagaa gtactacacg ttcactcgcg aaatgcaggt ggcgggggcg
     1141 cggtgcctgg tttcccgcac cggttatacc ggcgagatgg gctatgagct gtaccttgac
     1201 ccggccgatg cgtcgggcgt ctgggacgcg ctggtcgccg cgggcgcgac gccctgcggc
     1261 ctgggggcgc gggacaccct gcggctggag gcggcgatgc ccctttacgg ccacgagctg
     1321 tcggcgacca tcgaccccct cacggcgggg ctgggcttcg cggtcaagct ggaaaaaccg
     1381 gatttcatcg gcaagggggc cctgctggcc gtgggcgagc ccaagcgggt ccgcgccggg
     1441 ctgaaagtca ccggcagggg cgtgctgcgc gaggagcagg aggtttaccg ggacggcaaa
     1501 aaggtgggcg tgaccacctc cggcacccac tgcccggcgg tgggggtgag ctgtgccatg
     1561 gcctatctgg accgtgccgc cgcggccgtg ggcacagtgg tagaggtgga tgtgcgcggg
     1621 cgcagggtcg ccgccgaggt cgtgcccctg ccgttttaca aaaagtcaaa atgagagggc
     1681 gcagcgggaa agctccgcgc ccggcgcaaa agaaaaaacg aaaaatgaga tcaattttta
     1741 ggaggatatg acgatgaatt ttccggagaa cctgctctac agcaagtccc acgaatgggt
     1801 cgagaagacc ggcgagacga ccgcccgggt gggcctgacc gactatgcgc aggacgcgct
     1861 gggcgatctg gtctttgtca acctgcccga gccgggcgat gccgtcaccg acggcgagcc
     1921 ctttgcggac gtggagagcg tcaaggcggt gtctgacgtc tacagcccgg tcaccggcgc
     1981 ggtggcggcg gtcaacgaag agctgttgga ccgcccccag ctcatcaacg agagccctta
     2041 tgaggcctgg tttgtggagg tggaggagat tgcggccttt ggcgagctga tggacgccgc
     2101 cgcctacgag gcatactgca aggcggagca ggaaaaatga cccgtccccg cagcgggaca
     2161 gaaaggaagg gtgcagatgg gcagttatat cccgtccacc ggcgcgcagc gcgaggagat
     2221 gctgcgcgcg gtcggtgcgc cgtcgtttga ggcgctctat gcggacgtgc cgcaggagat
     2281 ccgtttcccc gcattgcaga tgggggaggg caggtcggag ttggaggtgt cggcggagct
     2341 gcgcgccatg gcggcgaaga atcatgtctt cagcagcatt ttccgcggcg cgggcgccta
     2401 tgaccactat atcccctcga tcgtcaagcg ggtgaccgca aaggaggagt tcgtcaccgc
     2461 ctataccccc tatcaggcgg agatcagcca gggcgtcctg caatccatct ttgaatacca
     2521 gaccatgatc tgcgagctca ccggtatggc cgcggccaac gcctcggtgt acgacggggc
     2581 cacggcggcg gcggaggccg cggctatgtg ccgcgaccgt cggcacagcc gggtgctggt
     2641 ctcggccgcc gcccaccccg ccgtgcgcag cacggtaaag acctattgcg agggggcggg
     2701 ggtgccgatg gttgaggtgc cggcccggga gggccgcact gacctggagg ccctgaaaga
     2761 gatgctcgcc gccggcgacg cctgctttta tctgcagcag ccgaactttt acggcgtgct
     2821 ggaggatgcg gccgcctttg gcgaggcggt gcatgcggcg ggcgcgaagt ttatcatggg
     2881 ggtcaacccc attgcggcgg cggcgctggc caccccggcg gagtgcggcg cggacatcgc
     2941 ggtgggcgag
//
LOCUS       DBMDYA010000349         2947 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7487_length_2947_cov_1.897048, whole genome shotgun sequence.
ACCESSION   DBMDYA010000349 DBMDYA010000000
VERSION     DBMDYA010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7487_length_2947_cov_1.897048"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="ACZPFK_08865"
     CDS             complement(<1..369)
                     /locus_tag="ACZPFK_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR1406463.1"
                     /translation="MRTPFKLRPAFKDYLWGGQRLRTVFGKQTDLSPLAESWELSVHK
                     DGPSIVASGEQAGRTLKEVLDADPEALGTACPEGGLPILIKLIDAKQQLSVQVHPTEE
                     YAMAVEHEHGKTEMWYVVQAD"
     gene            complement(416..1582)
                     /gene="alr"
                     /locus_tag="ACZPFK_08870"
     CDS             complement(416..1582)
                     /gene="alr"
                     /locus_tag="ACZPFK_08870"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1406464.1"
                     /translation="MEFEKRCWAEIDLDKIEHNFRVIKRRAPHSMIMAVVKADAYGHG
                     DAVIASTLEEAGADRFAVSGFAEALRLRRAGVTRPILVLGYTSPQKAAMLAINTITQT
                     VYSLEYARQLSDAAVAAGVTVNVHIKLDTGMGRIGFAVRDEMEAAVDQVCEATALPGL
                     CHIGIFTHFAVADSALPEDIAYTKRQFDLFCEAVRRLAAQGVTFPVAHCCNSAAILAY
                     PDMQLDMVRPGVILYGYRPSPDVTCGELTGALELKAAVSLVKSIRRGDDLSYGRIFTA
                     DRDMQVATLSVGYADGYQRALSNRGVVSIHGRPAPVVGRVCMDQIIVDVTAIPGVRQG
                     DVATIFGGGAADSVDDIADKCGTVNYEIICDIGRRVQRVYTRGGKPIQVTDYME"
     gene            2225..>2947
                     /locus_tag="ACZPFK_08875"
     CDS             2225..>2947
                     /locus_tag="ACZPFK_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1406465.1"
                     /translation="MRDLIVLDFETTGLDAGVDEVLQVSMVDGAGAVLMNEYCRPERH
                     ASWAAAQKINGITPEAVADKPPFRELLPRVLELLAGAKTVVAYNDEFERGFLAAYGVD
                     VRRLRWGKDPMKLFAAHFGNQRRTLSTVAAFFGYEFKAHDALEDTLATLEVYRHLSSG
                     PLLPHLMEAAVPAEEPGCRAFQQEADWLRLLKTLGLTPLTAKSRKPLAYKGVFAGGPV
                     ACEVIGFEYPALSDDSAVLLVQA"
BASE COUNT          495 a          913 c         1010 g          529 t
ORIGIN      
        1 gtccgcctgc acgacgtacc acatctcggt cttgccgtgc tcgtgttcca ccgccatggc
       61 gtattcctcg gtggggtgca cctgtaccga gagctgctgc ttggcgtcga tcagcttgat
      121 caaaatgggc aggccgccct ccgggcaggc ggtgcccagg gcttcggggt ccgcgtccag
      181 cacctctttc agggtgcggc ctgcctgttc gccgctggcg acgatactgg ggccgtcctt
      241 atgtacgctg agctcccagc tctcagcgag gggggacagg tccgtctgct tgccgaacac
      301 ggtgcgcagg cgctgccctc cccagaggta gtctttaaag gccggacgga gcttgaatgg
      361 tgtgcgcatg gtttaccctc cctgtatttc gattgccggc ggggagtgcc gccgtttact
      421 ccatataatc ggtcacctgg atcggcttgc cgccccgggt gtagacccgc tgcacgcggc
      481 ggccgatgtc gcagatgatc tcgtagttca ccgtgccgca cttgtcggcg atgtcgtcca
      541 ccgagtccgc cgcgccgccg ccgaagatgg tcgccacatc cccctggcgc acgcccggga
      601 tggcggtgac gtccacgatg atctggtcca tgcagacccg gcccaccacc ggcgcgggcc
      661 ggccgtggat cgagaccacg ccccggttgg acagcgcgcg ctgatagccg tcggcgtacc
      721 ccaccgagag ggtcgcgacc tgcatgtcgc ggtccgccgt gaagatgcgg ccgtagctca
      781 ggtcgtcccc gcgccggatg gacttcacca ggctgacggc ggctttcagc tccagcgcgc
      841 cggtcagctc gccgcaggtg acgtcggggc tggggcgata gccgtagagg atgaccccgg
      901 ggcgcaccat gtccagctgc atgtcgggat aagccaggat cgccgccgag ttgcagcagt
      961 gggcgacggg aaaggttacc ccctgcgccg ccagacggcg gacggcctca cagaacaggt
     1021 cgaactggcg cttggtatag gcgatgtcct ccggcaaggc ggagtccgcc accgcaaagt
     1081 gggtaaagat accgatgtgg cacagcccgg gcagggcggt ggcctcgcag acctggtcca
     1141 ccgcggcctc catctcgtcc cgcaccgcaa agccgatgcg ccccatgccg gtgtccagct
     1201 tgatgtggac gttgacggtg accccggcgg ccacggcggc gtcagacaac tggcgcgcat
     1261 attccagcga gtagacggtc tgggtgatgg tgttgatggc cagcatggcc gccttttgcg
     1321 ggctggtata gcccagcacc aggatggggc gggtgacccc cgcccggcgc agccgcagcg
     1381 cctcggcaaa gcccgagacc gcaaagcggt ccgccccggc ctcctccagc gtggaggcga
     1441 tgacggcgtc cccgtgcccg taggcgtcgg ccttgaccac ggccatgatc atgctgtggg
     1501 gggcgcggcg cttgatgacc ctgaagttgt gttctatttt gtccaggtcg atctcggccc
     1561 agcatctctt ctcaaattcc attctggaaa ctcctttttt ccctcgttca ggcggcggga
     1621 cgtcccatcc tctgcaaggc cagcatccgc accgtctgtt ttgcattagt atagcacaaa
     1681 agaggggcaa ataaaataaa agcgaggcgc cgaaaaaagc tttgccccca tgatggggac
     1741 gccgcgccgg gcggcggaag ctttccgcgc acgtttcttt ttgggcttaa tttgttattt
     1801 tgcaaaaaaa gtatacaaaa tggaaaaaat ggcacaaaac cgcccgccgc gacattttgc
     1861 acaggggagc cccggtatgg tataataact ttcaaaggga ggagaggcct gaccctgagg
     1921 gcgcaggtct tttttctgaa agagcgggca ggcggcccgc ggcccggcct ttgtaaaagc
     1981 cgctggaccg cccgggcgga acggcgctgt gcggctgtgc ctgcggcgcg ctcttttccg
     2041 tgcggagatc ggccgaaagc ggggagggca gttcctcccc gtctttgtgg gggatggccc
     2101 ggggcacgga taagctggtt ggacgcggcg gccccgggcg ggaggcagcg aaaaagcggg
     2161 ccggcctgcc cactacaaaa aagtctgcgg cgcagcgcgg gcgcagagga aacgcgtgga
     2221 aaaaatgaga gatttgatcg tgttggattt tgagaccacc ggcctggacg caggggtgga
     2281 cgaggtgctg caggtctcga tggtggacgg tgcgggcgcg gtgctgatga atgaatattg
     2341 ccgcccggaa cggcacgcct cttgggcggc agcgcagaag atcaacggca tcaccccgga
     2401 ggcggtggcg gacaagccgc cgtttcggga gctgctgccg cgggtgctgg agctgctggc
     2461 gggcgcgaag acggtggtgg cgtacaacga cgagttcgaa aggggctttt tggcggccta
     2521 cggcgtggac gtgcgcaggc tgcgctgggg caaagacccg atgaagctgt tcgccgccca
     2581 cttcggcaac cagcggcgca ccctctccac cgtggcggcc tttttcggct atgagttcaa
     2641 agcgcacgac gcgctggagg acactctggc cacgctggag gtctaccggc acctctcgtc
     2701 ggggccgctg ctgccccatt tgatggaggc cgccgtcccg gcggaggagc cgggctgccg
     2761 cgcctttcag caggaggcgg attggctgcg gctgctcaag acgctgggcc tcactccgct
     2821 gacggccaag agccgcaagc ctctggccta caagggcgtc tttgcgggcg gcccggtggc
     2881 ctgtgaggtg atcgggttcg agtatcccgc cctgtccgac gattcggccg tgctgctggt
     2941 gcaggcg
//
LOCUS       DBMDYA010000350         2946 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7490_length_2946_cov_1.508612, whole genome shotgun sequence.
ACCESSION   DBMDYA010000350 DBMDYA010000000
VERSION     DBMDYA010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_2946_cov_1.508612"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="ACZPFK_08880"
     CDS             <1..701
                     /locus_tag="ACZPFK_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406466.1"
                     /translation="MTDLREEKLYAQARGWAAQQGIEVLDFNLKKEELGIDPALDYAD
                     KSHLNYYGACKVSGAMGAYLAGTGLFQDKRQARGYEGWQRDAHCYETWEKVARLGETE
                     CSAAYAAVLAGLGGDYLAVLAGVPDETVSAALREAGLSVQPGVGFCALFDGKRWEVRR
                     GVPSLEGGTGGHRLAARCDAGGAVAEVDGSGITAKTGVSVLVYDALADKVADQAVLQG
                     EKTIHLPGPENDPA"
     gene            complement(829..1011)
                     /locus_tag="ACZPFK_08885"
     CDS             complement(829..1011)
                     /locus_tag="ACZPFK_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406467.1"
                     /translation="MKKWRIGALLLSLAGLSVMAAHHLIAPLPDWAVRSAGAALLIAL
                     PVLVFVSLRGRGGSQK"
     gene            complement(1054..1482)
                     /locus_tag="ACZPFK_08890"
     CDS             complement(1054..1482)
                     /locus_tag="ACZPFK_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406468.1"
                     /translation="MEQFKHKLERRIRLCQSGAGLALVAFFVGKYLGAPDEFSSGFRM
                     GVLLGLELCMVVLVIRTRRALCDEAALKEMYIRETDERNRQIKLMVFSSGWFFLLFCL
                     ALAILAASFFNFTVVFTLVGVLTCMCLIKLACTVYYRKKY"
     gene            complement(1469..1693)
                     /locus_tag="ACZPFK_08895"
     CDS             complement(1469..1693)
                     /locus_tag="ACZPFK_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406469.1"
                     /translation="MKNQIRELRKARRVSQEELAARVGVTRQTIISLENEKYTASLAL
                     AYKIARFFGCGIEEVFDFTEIEEEFQNGTI"
     gene            1877..2191
                     /locus_tag="ACZPFK_08900"
     CDS             1877..2191
                     /locus_tag="ACZPFK_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406470.1"
                     /translation="MWLIRSQRKKADGGGRGLEALRGKKFPGRRAPEFSAPLRRCGAG
                     GSFFQKAKEEKRGCGRAEAGDTRGKGRTRTRFAAPPRDYPEKRAAAKEKKADTAAFFA
                     AS"
     gene            complement(2268..>2946)
                     /locus_tag="ACZPFK_08905"
     CDS             complement(2268..>2946)
                     /locus_tag="ACZPFK_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406471.1"
                     /translation="LLPLYNFSRFRRVPLAPVIADGGGAMPLRELFSHRVFWLALLLM
                     MCAGSSELAMSQWSSLFAERGLGVPKMLGDLLGPCLFALLMGAGRTLYGVFGERLPLK
                     RLMAASSLFCVCCYLTTIFSPSPIISLLGCAACGLSVSLLWPGTFSLSAAALPRGGTA
                     LFSLLALAGDLGGAVGPWLAGLVSGHIAAGVGAEQAGLRGGLLAVTVFPLLMLGGVLS
                     FRREKRR"
BASE COUNT          625 a          875 c          998 g          448 t
ORIGIN      
        1 atatgaccga cctgcgggag gaaaagctgt atgcccaggc gcggggttgg gcggcgcagc
       61 aggggatcga ggtgctggac ttcaatttga aaaaggaaga gctggggatc gaccccgcgc
      121 tggactacgc ggataagagc catctcaact attacggcgc ctgcaaggtg agcggcgcca
      181 tgggggcata tctggcggga accggcttgt ttcaggacaa gcggcaagcg cgcgggtacg
      241 agggctggca gcgcgacgcc cattgttacg agacctggga aaaggtcgcc cgcctgggcg
      301 agacagaatg ttctgcagca tacgcggctg tcctggccgg cctcggcggc gactatctgg
      361 cggtcctggc gggagttccg gatgaaacgg tcagtgcggc cctgagggag gcggggcttt
      421 ccgtacagcc gggggtcggt ttttgcgcgc ttttcgacgg aaagcggtgg gaggtgcggc
      481 gcggtgtccc cagcctggag ggcggcacag gcggccaccg gctcgcggcc cgctgcgacg
      541 cggggggcgc ggtggcggag gtggacggca gcggtattac cgccaaaacc ggggtgagcg
      601 tgctggtgta tgacgccctc gccgacaaag tggcggatca ggctgttttg cagggagaga
      661 aaacgattca cctgcccgga ccagagaacg acccggcttg agcgagcctt tcccggggcg
      721 tccccaactc ctgcttctgg accgctttgt tttgccgggg aggctggccg cctgcccgct
      781 gcaggcaaac gcccctctct gccttggggc agagagaggc gttttgcgct atttctggga
      841 accgccgcgc ccgcgcaggg agacgaagac cagaacgggc agggcgatga gcagggccgc
      901 tccggcagag cggaccgccc agtccggcag gggggcgatc aggtgatgag ccgccatgac
      961 cgacaggccg gccagcgaca gcagcagggc gccgatcctc cattttttca tttttatctc
     1021 ctctcatcgc atgcccggcg tctgtttcaa aggtcaatat ttttttcggt aatagacggt
     1081 gcaggccagc ttgatcaggc acatgcaggt caggacgccg accagggtga agaccacggt
     1141 gaagttgaaa aagctggccg ccaggatggc cagggcgagg cagaacagca gaaaaaacca
     1201 gccgctggaa aagaccatca gcttgatctg ccggttgcgc tcgtcggtct cccggatgta
     1261 catctctttg agcgccgcct cgtcgcagag cgcgcgccgg gtgcggatga ccaggacgac
     1321 catgcagagc tccagcccca gaagcacgcc catgcggaag ccggaagaaa actcgtcggg
     1381 cgcgccgaga tattttccca caaagaaagc gaccagggca aggcccgccc cgctctggca
     1441 aaggcggatg cgccgctcga gcttgtgttt aaattgttcc attctgaaat tcctcctcga
     1501 tttcggtaaa atcaaaaacc tcttcgatgc cgcacccaaa aaagcgcgcg atcttgtaag
     1561 cgagcgccag gctggccgtg tacttttcat tttcaaggct gatgatagtc tggcgcgtca
     1621 ccccgacgcg ggcggcgagc tcttcctggc tgacacgccg cgccttgcgc agctcccgta
     1681 tctgattttt cacggagaca ctcctcaact gctgttttga attgtcaagt cgactttaca
     1741 atgaaagtat agcgcgcttt acataaaatg tcaagtagac tatacaaaaa actgtggata
     1801 agacaggtgg ccgaggggag gggcggcagg cctacccaca accacaagag agcggccccg
     1861 tgggagggcc gctctcttgt ggttgattcg atcacaacgg aaaaaagccg atgggggcgg
     1921 gcgcggcctg gaagcgctgc ggggcaagaa atttcccggc aggcgcgcgc ccgaattttc
     1981 cgctcctctc cggcgttgcg gtgcgggagg ttcatttttt caaaaagcga aagaagaaaa
     2041 aagagggtgc gggcgggccg aagcggggga tacacggggg aaaggccgaa cgcggacccg
     2101 ctttgccgcg ccaccgcgcg attacccgga aaaacgcgcg gcggcgaaag agaagaaagc
     2161 agacaccgca gccttttttg ctgcatcatg acgcgggaag acgcttgcgg cggggaggga
     2221 aaagggccgg gcagagcggc cgcgccgcag gggccgcccc agaggcttca acgccttttt
     2281 tcgcgtcgaa acgacagcac accgcccagc atgagcaagg ggaagaccgt caccgccagc
     2341 agcccgccgc gcaggcccgc ctgctcggcc ccgaccccgg ctgcgatgtg gccggagacc
     2401 aaaccggcca gccaggggcc caccgcgccg cccaggtcgc cggccagggc gagcagactg
     2461 aacaatgcgg tgccgccccg cggcagcgcc gcagcggaga ggctgaacgt gcccggccag
     2521 agcaggctga ccgagaggcc gcaggccgcg cagcccagca gcgagatgat cgggctgggg
     2581 gagaagatgg tggtgagata gcagcacaca cagaaaaggc tgctggcggc catcagcctt
     2641 ttcagcggca gccgctcgcc gaagacgccg taaagggtgc gcccggcgcc catcagcagg
     2701 gcaaacaggc aggggcccaa aaggtcgccc agcattttgg gcaccccgag cccgcgctcg
     2761 gcaaagaggc tgctccactg gctcatcgcc agctcgctgg aaccggcgca catcatcagc
     2821 agcagggcca gccagaacac gcggtgagaa aagagctccc gcaagggcat ggcaccgccc
     2881 ccgtcggcga tcacaggggc cagcggcacc ctgcgaaaac gcgagaaatt gtaaagaggc
     2941 agcagg
//
LOCUS       DBMDYA010000351         2944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7495_length_2944_cov_1.556454, whole genome shotgun sequence.
ACCESSION   DBMDYA010000351 DBMDYA010000000
VERSION     DBMDYA010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7495_length_2944_cov_1.556454"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="ACZPFK_08910"
     CDS             complement(<1..601)
                     /locus_tag="ACZPFK_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1406472.1"
                     /translation="MNIREVVMKRIDESADEYLEFLKKVVSADTRILEHGRLGHEDNG
                     QRIIRAFLEQSGAQVDSFQPTYEQMAFCPEVNPDHDYEGRHNVVGVFSGTGGGRSLLF
                     NGHIDTVDFGDVNQWTRGPLEPYVEDGKLVGRGACDMKGGLCASLMAMKALRDCGVRL
                     KGDVLYESVIDEEGGGNGTLACCAKGYRADAAIIPEPSDL"
     gene            complement(938..2041)
                     /locus_tag="ACZPFK_08915"
     CDS             complement(938..2041)
                     /locus_tag="ACZPFK_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406473.1"
                     /translation="MARTEVPLYVKIKEYLLREIREGELKPDDQLPSEKALSEQFQVS
                     RITVRRALAELEEESAIYRRPGKGTFVAEAPQAGEAVKTPLPAAGEGPVIGVIMSHLD
                     SPFQVSLLQSIEKSIGKKACQMMFGLSNGSTKIENELIDRMLAHGVQGMVIYPVDGAF
                     YSEKILRMSMDGFPVVLVDRYLPGINACSVYSDDRRGGQLLGEYLLGRGHRRIGLLSQ
                     DPKDTICLMDRIDGFNSAMLAAGVPQGAEDMMTDITNCAVYSDPEQYEENVEKIERFL
                     TGRPEITAVFCTIATFAMNAVWAARRLDRRLEIVCFDSVVPYQWTEKFPITYIAHSET
                     EMGRRAVEAVLRLIAGEKAENIILPCELVPAPL"
     gene            2273..2524
                     /locus_tag="ACZPFK_08920"
     CDS             2273..2524
                     /locus_tag="ACZPFK_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406474.1"
                     /translation="MSVFHLSSFFSNISFFARGLVGLGVLIGAYFYSGRQRLLKNAAI
                     LFGCAFGALMVLRVLLGVLGVWAALLGWMQGGALPHTLY"
BASE COUNT          530 a          932 c          827 g          655 t
ORIGIN      
        1 agaggtcgga gggctcgggg atgatggccg cgtcggcccg gtatcccttg gcgcagcagg
       61 ccagcgtgcc gttgccgccg ccctcctcgt cgatcaccga ttcgtacagc acgtcccctt
      121 tcagccgcac cccgcagtcg cgcagggctt tcatggccat caaagaggcg caaagccccc
      181 ctttcatgtc gcaggcgccg cgccccacca gcttgccgtc ctccacatag ggctccagcg
      241 ggccgcgggt ccactggttc acatcgccga aatccaccgt gtcgatatgg ccgttgaaca
      301 gcagcgagcg cccgccccct gtgccggaaa aaacgcccac cacgttgtgc cgtccctcat
      361 agtcgtgatc ggggttgacc tccgggcaaa acgccatctg ctcataggtg ggctgaaagc
      421 tgtccacctg cgcgccgctc tgttccagaa aagcgcggat gatcctctgc ccgttgtcct
      481 cgtgccccag cctgccgtgt tccaggatgc gggtgtcggc ggagaccacc tttttgagga
      541 attccagata ctcgtctgcg ctttcgtcga tccgcttcat cacgacttct cttatgttca
      601 tctgctcgtc tctcctccat gctgcccgaa tttgcaagtt tattgtatca ttacatgtat
      661 ataatgtcaa ttgtatttta ttacatgact tatatcatat ttttataggt attgcccaaa
      721 cccattccct gacagaggcc tccctcgggg gaagaaaccg attttctgcg ccgccgcatt
      781 cttgcccctc cagatgccgc acggcttgaa agaccggaag cagcgtctat tttgctgtcc
      841 tgtagcagca aaaaagagcg tcccggcact cgaagtgtcg ggacgctctt tttttgtctt
      901 ttatccttgt tcccaaagcg cggcagggcg cctcatgtca gagcggggcg ggaaccagct
      961 cacagggcag tatgatgttc tcagcctttt cacccgcaat cagccgcagc accgcttcta
     1021 ccgcccgcct gcccatctcg gtctccgaat gggcgatata agtaatgggg aatttttcag
     1081 tccattgata gggcacgacg ctgtcgaagc agacgatctc cagccgcctg tccagcctgc
     1141 gggccgccca cacggcattc atggcaaagg tggcgatggt gcagaagacc gcggtgatct
     1201 caggacggcc ggtcagaaac cgctcgatct tttccacatt ctcctcgtac tgctccgggt
     1261 cgctgtacac cgcacagttg gtgatgtccg tcatcatgtc ctctgcgccc tgcggcaccc
     1321 cggcggcaag catagcgctg ttgaagccgt cgatgcggtc catgaggcag atcgtgtcct
     1381 tggggtcctg cgagagcagt ccaatgcgcc ggtggccccg gcccagcagg tattctccca
     1441 ggagctggcc cccccgccgg tcgtccgaat agacgctgca ggcattgatg ccgggcagat
     1501 aacggtccac cagcaccacc gggaagccgt ccatgctcat gcgcaggatt ttttcactgt
     1561 aaaaggcccc gtccacggga tagatcacca tgccctgtac cccgtgggca agcatgcggt
     1621 cgatgagttc gttctcgatc ttcgtgctgc cgttggagag gccgaacatc atctggcagg
     1681 cctttttacc gatcgacttt tcaatggact ggagcagcga cacctggaac ggggagtcca
     1741 gatggctcat aatcacgccg atgaccggcc cctcccccgc ggcgggcaga ggggtcttta
     1801 ccgcctctcc cgcctggggg gcctcggcca caaaggtccc tttcccaggg cggcggtaga
     1861 tggcgctctc ctcctccagc tcggccagcg cacggcgcac ggtgatgcgg ctgacctgga
     1921 actgttctga cagggctttt tcggacggga gctggtcgtc gggcttcagc tccccctcac
     1981 ggatctcccg cagcagatat tctttgatct tgacatataa aggcacttct gtccttgcca
     2041 tcttgttccc tctttcagcg cgagcggaag ttatatttgt tatatttgta atatatcatg
     2101 tatcctattg gctgtcaaga aatcccaaac tattttttca ccgggcaggg aaaaatctcc
     2161 ctctccgccg ctttttcgcg ctctcgttta caaagcgggt aaaaaacggt atactgaatc
     2221 aaatccaact ttacacaggc cggagacgcc tgtgcaggga ggtctgtcgc cgatgtctgt
     2281 ctttcatctc tccagctttt tttccaacat cagttttttt gcccgcgggc tggtgggcct
     2341 gggggtgttg atcggggctt atttttacag cggccggcaa agattgctga aaaatgcggc
     2401 catccttttt ggctgcgctt tcggcgcgct gatggtgctg cgggtgttgt tgggcgtgct
     2461 gggcgtctgg gccgcgctgc tgggctggat gcagggcggc gctttgcccc acaccctgta
     2521 ctgacccgcc gctctctccg cggccgcgcc cgtcggccgt ctttacggcc gcgcccaagc
     2581 ggtgcgcgcc ctcaaagcgc cgccccaccg cggccttttt tcgccgcagg gccgcccgca
     2641 aacagggcgc gcgaagaggc ggagcgtaaa aaagcggccg ggagaaagca cccccgccgc
     2701 caggcgccgt cccggcggcc aggcgtcttt ccgcgggaaa gggacggggc cgctctcttt
     2761 ctgcgggagg gcaaaatgcg aggcaacaaa aaacaggagg cggggactca ttcgccccgc
     2821 ctcctgtttt gcccccccgg gggccgcgcc ctcatcacag gtagactttc atctcctcga
     2881 tgttgcgctg catgaactga accatgcggt cggccagcgc aacatcgagg agatgaaagt
     2941 caga
//
LOCUS       DBMDYA010000352         2943 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7498_length_2943_cov_1.395496, whole genome shotgun sequence.
ACCESSION   DBMDYA010000352 DBMDYA010000000
VERSION     DBMDYA010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_2943_cov_1.395496"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="ACZPFK_08925"
     CDS             complement(<1..626)
                     /locus_tag="ACZPFK_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1406475.1"
                     /translation="MDYREYYRADAKRRRRKRGFQRFCFFALLLLLLLGLAAVITWVI
                     EAVGGKNDSSASLPGPGSSSAVSSSLPDESGSASSGAQQGAALPEKGRVELSYFTDAA
                     FVGDSITVGWSDYKGAAALPDTHVIAAIGVTPPVNGVQWANADGTTYDPLQAIADAAP
                     AKLYLMFGANKLVDQGDAAEDTLLSSYATFIDDLKAKLPQAKIYIQSIL"
     gene            complement(879..1889)
                     /locus_tag="ACZPFK_08930"
     CDS             complement(879..1889)
                     /locus_tag="ACZPFK_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1406476.1"
                     /translation="MAGYREFRAAERRKRRLRRARAAALVLLAAVLLAGGVFAAVKFW
                     KGGQPSDAPSGAGPSASAPNPGGGQASAASGSQTAGAQGGAPNSSAFSRWEEMALAGQ
                     TINGFSPLSPNRRLYALPQNGRVDVSYFDDAVFVGDSISTGWSVYREASGMLPNPNVV
                     AEKGASPPANGSQWARNQNAGDLYDPLEAIVALSPKKIYVMLGANMLVAEGEGVEDRL
                     VSSYGVFIDDLRARLPGVKIYVQSILLPTAEYSAAHAGLTPERINRVNDRLAALSFEK
                     NCYYLDLEEYLCKDGVLNWDIAQPDGLHINTDGYRGWLEYLVTHTAYDPANPYTETPQ
                     HY"
BASE COUNT          500 a         1008 c          913 g          522 t
ORIGIN      
        1 agtatcgact ggatataaat tttggcctgc ggaagcttgg ctttcaggtc gtcgataaag
       61 gtggcgtagc tggacagcag cgtgtcctcg gccgcgtcgc cctgatccac cagcttattc
      121 gcgccgaaca tcagatagag cttggccggc gcggcgtccg caatcgcctg cagcgggtca
      181 taggtcgtgc cgtcggcgtt cgcccactgc acgccgttca ccggcggcgt gacgccgatg
      241 gccgcgatca catgggtgtc cggcagggcc gccgcgccct tgtaatcgct ccagcccacg
      301 gtgatcgagt cgcccacaaa ggcggcgtcg gtgaaatagg acagctccac ccggcctttc
      361 tccggcaggg cggcgccctg ctgcgctccg ctggaagcgg agccgctctc atccggcagc
      421 gaggatgaga cggcggagga ggagcccggc cccggcagcg aggcggaaga atcgtttttg
      481 ccgcccaccg cctcgatgac ccaggtgatc accgccgcaa ggcccagcag caacagcaaa
      541 agaagcgcaa aaaagcaaaa gcgctgaaac ccgcgcttgc ggcggcggcg cttggcgtcc
      601 gcccggtaat actccctgta atccaaagga tatcaactcc gtgacatttc tttcggcgcc
      661 cggcgcgggc cgcgcctggg ggcgggcgcg cgccgggaat actccatatt attacacact
      721 tgggccgtgg gtcaatccgg gcgggcggcg gagaaagcgc cgccgggcgc gcagacgccc
      781 ttttcagccg cggggctttt ttcgggccgg agaggcggtc cccccgcaaa gcgctccgtc
      841 cctcaaaaaa gcggccccgt gcccccccgc gccgccgctc aatagtgctg gggcgtctcg
      901 gtataggggt tggccggatc gtaggcggta tgggtgacca ggtactccag ccagccccgg
      961 taaccgtcgg tgttgatgtg cagaccgtcc ggctgggcga tgtcccagtt cagcaccccg
     1021 tccttgcaga gatattcctc caggtcgaga taatagcaat ttttttcaaa ggacagcgcg
     1081 gccagccggt cgttcacccg gttgatgcgc tccggcgtca gccccgcgtg cgcggcgctg
     1141 tactcggcgg tgggcagcag gatagactgc acatagatct tcacgccggg cagccgggcc
     1201 cgcaggtcgt cgataaagac gccgtagctg ctcaccagcc tgtcctcgac cccctcgccc
     1261 tcggccacca gcatgttggc ccccagcatg acataaatct ttttcggcga gagggccaca
     1321 atcgcctcca acgggtcgta gaggtcgccg gcgttctggt tgcgcgccca ctggctgccg
     1381 ttcgccgggg gcgaagcgcc cttttccgcc accacgttgg ggtttggcag catgccgctt
     1441 gcctcccggt aaacgctcca gccggtgctg atggaatccc ccacaaagac cgcgtcgtca
     1501 aaataggaga cgtccacccg gccgttctgg ggcagggcgt agagcctgcg gttggggctc
     1561 agcggcgaaa agccgttgat cgtctgcccg gcgagggcca tctcctccca gcgggagaag
     1621 gcgctggaat ttggcgcgcc cccctgcgcc cccgccgtct gggagccgga agccgccgag
     1681 gcctgtccgc cgcccgggtt cggagccgac gcggacgggc ccgccccaga gggcgcgtcg
     1741 gacggctgcc cgcctttcca aaattttacc gccgcaaaga cgccgcccgc cagcagcacc
     1801 gccgccagca gcaccagcgc cgcggcccgc gctctgcgca gccgccgttt acgccgctca
     1861 gcggcgcgga actcacgata tccggccatg ccgtcccctc cctgtctctt ccgttttctc
     1921 ccgcgcctgc cgggcacgtc ccggccgatc ccgttcgccc ggcggcgggg aggcggcgtt
     1981 gttttgattt tcaaaaatac tttttccggg cgttttgcag ggcggcgttt tttaaaaacg
     2041 gctttcccgc agagccgccg cgctgcgggc cgcggcgcag ggcgcgcgac gccggaaaag
     2101 cccactgccg cagcgtccca cccacaggga cgccccgccg cccccattcc ctacagagcg
     2161 gccgcttttt cgaggatggg cccggtatcc ccccgtcagg atcagcactt ccgacgctga
     2221 agcttcctcc gcgccctgtc cgccgcaacc gccgcacagc agctctctgg aaagttttga
     2281 gccaagcgct cggggcggtc gccgcgggag cacaagagcc tgcttcggcg ctttgctccc
     2341 accggggcgg tcttatcggt tcgtctccga ttggccctcc ctgcgcggca tgacattttt
     2401 tacgttcgct gacctgtttg ccctctccag cccccttccg gctcccgcag agcggaagcg
     2461 gcctcccctg gtcaagggaa gccggggctg ggcgcctgga gcgatccccc tcgccggaga
     2521 cggcgctcct ctccaatccc gcttctgtcg gcggcgctct cagggcgccc ctttcagggg
     2581 aggcggcgcg caagcggccg agggacaatt tcaaagccgt gcccgaaagt cctcgcacag
     2641 tcatgattca aaggcggccc ttgtccgaag aaaaagcggc tcaaggaact gaaatctcga
     2701 gcgcccgccc aaggagcggg cggatgcgcg taaaaaatag cccgcggctg ttttttaccg
     2761 ctgcgtaata aaaaacaatt tatttctatg gccgtggccc gagacgggcc acatggccta
     2821 aaaaatagcc tgcggctatt ttttattgct ccgcaataaa aaataattta tctctatggc
     2881 cccggccagg ggactggccg gatgaccgca aaaaatagcc tgcggctatt ttttattgct
     2941 ccg
//
LOCUS       DBMDYA010000353         2941 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7503_length_2941_cov_1.212676, whole genome shotgun sequence.
ACCESSION   DBMDYA010000353 DBMDYA010000000
VERSION     DBMDYA010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_2941_cov_1.212676"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="ACZPFK_08935"
     CDS             complement(<1..247)
                     /locus_tag="ACZPFK_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR1406477.1"
                     /translation="MNLLVTLDANYIPVLRVMLYSLLKNNPGEHFCLYIAHSTLTEEH
                     LASILRGLDSARLALVPVPVDDSALAGAPIEKRYPREM"
     gene            498..2120
                     /locus_tag="ACZPFK_08940"
     CDS             498..2120
                     /locus_tag="ACZPFK_08940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1406478.1"
                     /translation="MKDKRFVAVVASFLLLFAVLQTRLVGLAMNTQSAQAAAVQSSCL
                     LPLYGGRAEIYDCRMRPLTGVATEQMALALPGDDSYAKLYRSLAEDTAAQLYSLAGTK
                     PSLVGLASQVDESLGIYTFDKPRRYFEAPIAVHTLGYLGQDGSGVSGIERCYDELLAG
                     CGDSTFVRCTTTAAGGLMAGTVPELVRRSGSGLSVQLTLDRDLQRVCEGIALEAVPHG
                     AIVVMDVETGELRAVVSTPGYDPYDVAKSIEANDSGLLNRAISAYSVGSVFKPLVAAA
                     ALKAGMDENAVYECTGVIEVDGHLYHCNNHKAHGQMTMQSALEQSCNCYFIWLGQQLG
                     GEAICELATLEGFGRATPLAEGYYGASGNLPGAEELKNSGQLASVSFGQGRLLATPVQ
                     LAACFNTFANDGVYIGPTLVRGKVEAATGKIVEAGPAQETMRVLPQQLNDKLQRMLRG
                     VVEEGIAGKAKPAQGGAAGKTGTAQTGRADGEGNELLDSWFAGYYPAEEPRYTIVILK
                     DSTHEAGETLAPVFARLCDALTLAESAEDTAG"
     assembly_gap    2295..2311
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2397..>2941
                     /locus_tag="ACZPFK_08945"
     CDS             2397..>2941
                     /locus_tag="ACZPFK_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR1406479.1"
                     /translation="MKWTGLNELRESFLSFFESKGHLRLDSFPLVPKDDNSLLLINSG
                     MAPMKKWFLAQEEPPRHRVCTCQKCIRTPDIERVGITARHGTFFEMLGNFSFQDYFKE
                     EAIAWGWEYLTQVLEIPAEKLYISVYLDDDEAYDIWTQKIGIPEDHMVRFGNEDNFWE
                     HGSGPCGPCSEIYFDRGPEHG"
BASE COUNT          568 a          841 c         1016 g          499 t
ORIGIN      
        1 acatctcgcg gggatagcgt ttttcgatgg gagcgcccgc gagggcgctg tcgtccaccg
       61 gcaccggcac cagggccagc cgcgcgctgt ccaacccgcg caggatagag gccagatgct
      121 cttcggtgag ggtgctgtgg gcgatataca ggcaaaagtg ttcgcccggg ttgtttttca
      181 gcagggaata gagcatcacc cgcaggacag ggatgtagtt tgcatccagt gtgacgagca
      241 gattcatcga tcgttcctcc aaccatagta atactttttt aaatgccggc aagggggccg
      301 cccgtgataa aaagcgccct gcgcccggct gctccgccag aggcgcggat gggcgttagt
      361 tacaaataaa tataaaatga ccgcagtacg gctattttac catattgagg ggcggcgatt
      421 caatcgaaac atgccgccgg attgcttaaa gcccaggaga agagcacaga atagagaaaa
      481 aaagggggcg gatacgggtg aaagacaagc gcttcgtggc tgtggtggcg tcctttctgc
      541 tgctgttcgc cgtgctgcag acgcggctgg tggggttggc gatgaacacc caatcggccc
      601 aggcggcggc ggtgcagagc agctgcctgc tgccgctgta cggcggccgg gcggagatct
      661 acgactgccg catgcgcccg ctgaccgggg tggcgaccga gcagatggcg ctggcactgc
      721 cgggggacga cagctatgcc aagctctatc gctcgctcgc agaggacacg gcggcacagc
      781 tctacagcct tgcggggaca aagccgtccc tggtggggtt ggcctcacag gtggacgaga
      841 gcctcggcat ctataccttt gacaagccgc gccggtactt tgaggcgccc atcgccgtgc
      901 acactctggg ctatctgggg caggatggaa gcggcgtctc gggcatcgag cgctgctatg
      961 acgaactgct ggccgggtgc ggggacagca cctttgtgcg ctgcaccacc acggccgcgg
     1021 gcggcctgat ggcggggaca gtgccggagc tggtgcgccg gagcgggagc gggctttccg
     1081 tccagctgac gctggaccgg gacctgcagc gggtgtgcga ggggatcgcg ctggaggccg
     1141 ttccccacgg ggccatcgtg gtgatggacg tcgagaccgg tgagctgcgc gccgttgtca
     1201 gcacgccggg ctatgacccc tacgatgtgg ccaagagcat tgaggcgaac gacagcggcc
     1261 tgctgaaccg ggccatcagc gcctacagcg tggggtcggt gttcaagccg ctggtggctg
     1321 cggcggccct caaggcgggg atggatgaga atgcagtata tgagtgcacg ggtgtgatcg
     1381 aggtggacgg gcacctgtac cactgcaaca atcacaaggc gcacgggcag atgaccatgc
     1441 agtcggcgct ggagcaatcc tgcaactgct attttatctg gctgggccaa cagctgggcg
     1501 gggaggcgat ctgtgagctg gccacgctgg agggctttgg gcgggcgacc ccgctggccg
     1561 agggctacta tggggccagc ggcaacctgc ccggcgcgga agagctgaaa aacagcggcc
     1621 agctcgcctc ggtcagcttt ggccaggggc ggctgctggc gacgccggtg cagctggcgg
     1681 cctgcttcaa cacctttgcc aacgacggcg tgtatatcgg ccccaccctg gtgcggggca
     1741 aggtggaggc cgccaccggc aagatcgtgg aggccgggcc cgctcaggag acgatgcggg
     1801 tgctgccgca gcagctcaac gacaagctgc aaaggatgct gcgcggggtg gtggaggagg
     1861 gcatcgccgg caaggcgaag cccgcgcagg ggggcgcggc gggcaagacc ggcaccgccc
     1921 agaccggccg cgccgacggc gagggcaacg agctgctgga cagctggttt gcaggctatt
     1981 acccggcgga ggaaccgcgc tacaccatcg tcatcctcaa ggattccacc catgaggcgg
     2041 gcgaaacgct ggcgccggtc tttgcgcgcc tttgcgacgc gctcaccctg gcggagagcg
     2101 ccgaggacac ggcgggctga gccgctgctg tgggcggccg cgaaaaggcg cgcaaaaggc
     2161 cgcttttgct tgacaagcgc ggccgggttc cctataattg atttgtatgg ccggcggcac
     2221 agacgggttc ttgcaaagcc ggcaaaatgg tctgtccgat caagaaaaaa gcgatgacgg
     2281 ggcccatggc cggtnnnnnn nnnnnnnnnn ncctgactgg gggcggaggc ttaacctttt
     2341 ttaagggccg tgtgcccggc tgtgaaagac aacctgaaag gagtacgtga ataaagatga
     2401 aatggactgg tctcaacgag ctgcgcgagt cttttctctc tttttttgag agcaagggcc
     2461 atctgcggct ggacagcttt ccccttgtac ccaaggacga caacagcctg ctgctgatca
     2521 acagcggcat ggcgccgatg aaaaagtggt ttctcgccca ggaggagccg ccccgccacc
     2581 gggtctgcac ctgccagaaa tgcatccgca ccccggatat cgagcgggtg ggcatcacgg
     2641 cgcggcacgg cacctttttt gagatgctgg gcaacttcag ctttcaggat tatttcaaag
     2701 aggaggccat cgcctggggc tgggagtatc tgacccaggt gctggagatc ccggccgaaa
     2761 agctgtacat ctcggtctat ctggacgacg acgaggccta tgacatctgg acccagaaga
     2821 tcggcatccc tgaggaccac atggtccgct tcggcaatga ggacaacttc tgggagcacg
     2881 gctccggccc ctgcggcccc tgcagcgaga tctacttcga ccgcggcccc gagcacggct
     2941 g
//
LOCUS       DBMDYA010000354         2939 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7506_length_2939_cov_1.578576, whole genome shotgun sequence.
ACCESSION   DBMDYA010000354 DBMDYA010000000
VERSION     DBMDYA010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_2939_cov_1.578576"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="ACZPFK_08950"
     CDS             <1..1092
                     /locus_tag="ACZPFK_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406480.1"
                     /translation="PQRPQQAPYGMEQRQEGERRQPPERSAPHTLPQPWEQREPQPVE
                     PPQGGAATEPSRLALYGPREQEKMLRLLREKGLQTLLAGDTLEERNDGGQALLRLAKL
                     GADENAKASEEQSGGAEYEPSSHKWDVAREWGVDPDELMWDEELKRFLTPEEIEKRIW
                     GDKEENQRLFPIDFPYLTPEMPREKEKTSKGDYEAFLQDFSSGESGRDYEAELINNPS
                     DPYSWYFGKYQIGMEVMQTHGYIDANGNWTQKAREDGIESWEDFKKAPEFQEKVKRQV
                     DERFWQDCVALGLTKYIGTKVGQDQILVTASGLLAAANSKGPTGLKNIFEHNLWGTDR
                     EKDGNGKPVSDYLRDFGGYDISIITGGGY"
     gene            1620..2036
                     /locus_tag="ACZPFK_08955"
     CDS             1620..2036
                     /locus_tag="ACZPFK_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406481.1"
                     /translation="MLLSVGGGIVSTYYNKDSAVEGYGGAEYWAVCASGEKQCAGAAI
                     SPQQGLCLVAERLWKDEAVLVGTEARLRAVHVVNGKEEAHLDWALGPVEKDGGIFYRY
                     YTSGLFSDHWEDFYLVDPGSGEVYRETEDGMETVGP"
     gene            complement(2210..2584)
                     /locus_tag="ACZPFK_08960"
     CDS             complement(2210..2584)
                     /locus_tag="ACZPFK_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1406482.1"
                     /translation="MAEIISTQNAPAAIGPYSQAVKANGMLYTSGQIGIVPATGELAE
                     GGLAAQAEQVMKNLAAVFAAAGTGFEKAVKTTCYLADMADFAAFNEVYARYFVGKPAR
                     SCFAVKGLPKGALCEVEVVAEL"
BASE COUNT          671 a          725 c          965 g          578 t
ORIGIN      
        1 ccgcagcggc cgcagcaggc tccgtatgga atggaacagc ggcaagaggg cgagcggcgg
       61 cagccgccgg agaggagcgc gccccacacc ctgccgcagc cgtgggaaca gagggaaccg
      121 cagccggtgg aacccccgca gggcggcgcg gccacagagc cgtcccgttt ggcgctgtac
      181 ggcccgcggg agcaggaaaa aatgttgcgg ctgctgaggg aaaagggatt gcaaacgttg
      241 cttgcaggtg atacgcttga ggaaagaaac gacggcggac aggccctgct gcggctggcc
      301 aagctgggcg cggacgagaa cgccaaagcg tccgaggagc agagcggcgg ggcggaatac
      361 gagccgtcct cgcacaagtg ggatgtggcg agagaatggg gtgtcgatcc ggatgagctg
      421 atgtgggacg aagagttgaa gcgatttctt acgccagaag aaattgaaaa acggatatgg
      481 ggagacaagg aagaaaatca gcgccttttc ccgatagact tcccttattt gacgccagaa
      541 atgccccgcg aaaaagaaaa aacatctaaa ggagactacg aagcattttt gcaagatttt
      601 tccagtggtg aatccggtcg ggattatgaa gcggagctta tcaacaaccc ttctgatccc
      661 tatagctggt attttggtaa gtaccagatc ggaatggaag taatgcagac acatggatat
      721 atagatgcca atgggaattg gacacaaaaa gcacgagaag acggtatcga gagttgggag
      781 gattttaaaa aagcgccaga gtttcaagag aaagtgaaga gacaagtaga tgaacgattt
      841 tggcaagatt gtgtagctct gggacttaca aaatatatcg ggacaaaagt tgggcaagat
      901 cagattcttg ttacggcctc tggccttctt gctgcagcta actcaaaagg acctaccgga
      961 cttaaaaata tttttgagca caatttatgg ggaacagacc gggaaaaaga tggaaacggt
     1021 aagccggtca gcgattatct tcgagatttt ggcggatatg atataagcat cattacaggc
     1081 ggagggtatt aaaggatgaa aaaaagggtg cggtttttgg gactgctgat tgtggtctta
     1141 ttctccttgt cgggctgtgc cgcggcgggg ggagggagcc tttcctctga ggcgggcggc
     1201 gaagctgcgc ccgcgcccgt cccggcgccg aagctgctgg acgtggagac gggagctctt
     1261 gagatcacgg gggcgccttt ggacggcttg gagctggatt ttatccggga ggaattcaag
     1321 gatattgcag cgctgaaaga aagggaggac gcggcctacc ggcgggggga ggctgcggcg
     1381 ccctcgacgc tgggcgtcct tgctttttct tgcgatctcg atgaaaacgg acagacggat
     1441 tatctggctt ttccggtggg gttcgggtat acggcgacga tggatcagca ttccacaggc
     1501 gttttttttg tagcggacgc ggggggatac cgcacctttt ccggcgttcc ttttgccgca
     1561 gtccattcgc tgaaaatcta tcttttagag agcaaaaccg gggggatgca cgatctgttt
     1621 tgctgttatc cgtcgggggt ggcatcgttt cgacctatta taacaaggac agcgcggtgg
     1681 agggatacgg gggcgccgag tattgggcgg tttgcgcgtc gggggaaaag caatgcgccg
     1741 gcgcggcgat ctctccgcag cagggcttgt gtctggtcgc ggaacggctc tggaaagacg
     1801 aggcggtcct agtcgggacg gaggcgcgcc tgcgggcggt tcatgtggta aacggcaaag
     1861 aagaggcgca tctcgattgg gccttggggc cggtagagaa agacggcggg atattttacc
     1921 gctattatac cagcgggctc ttctcggatc attgggagga tttttatctt gtggaccccg
     1981 ggagcgggga ggtctaccgg gagacagagg acggcatgga gacggtgggg ccttgagcgc
     2041 ggcgctcgcg caaaagacca ttttgagaat tcgtgttttt gagatctgca aaattgaaaa
     2101 gaagaccggc ggagagggct tctccgccgg tcttcttttc aattttgctt tgggtgggct
     2161 gtcggcggcg ccgacacggc gaaaagcttg ccgccgcaag cggccgcgct cacagctcag
     2221 cgacgacctc gacctcgcac agggcgccct tgggcaggcc ctttaccgca aaacagctgc
     2281 gggcgggctt gcccacaaag tagcgggcat aaacctcgtt gaacgccgcg aaatccgcca
     2341 tatccgccag ataacaggtg gtcttgacgg ccttttcaaa tccggtcccc gccgcagcga
     2401 aaacggccgc cagatttttc atcacctgct ccgcctgcgc ggcgaggccg ccctctgcca
     2461 gctcgccggt ggcgggcaca atgccgatct ggcccgaggt gtaaagcatg ccgttggcct
     2521 tgaccgcctg cgagtagggg ccgatggctg cgggcgcgtt ttgagtgctg atgatctctg
     2581 ccatatccat tcacctgtcc tttgccaaaa gtttgtgatc ccagtatacc acgccgccgc
     2641 ccgcgggtaa aggcggtcag agcagcaaaa cgctgtttcg ccgcacgccg ggcggcgaag
     2701 gcttgcccgg cgcggaagaa aacacccgtc ccccggcggc gtgaccggag agacgcaaaa
     2761 ggcgagcccg gtacaggcgc gccttttgaa gagatagcag gatgaaaaat ctttttcagg
     2821 cctcgggaaa gagggggata aattcttttt tcatcacgtc caccagaccg aggtcgctaa
     2881 acttggcctc cggcaccacg ggcagcgcca gggtggcgat gcgcagcagc gggttcacc
//
LOCUS       DBMDYA010000355         2930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7528_length_2930_cov_1.394486, whole genome shotgun sequence.
ACCESSION   DBMDYA010000355 DBMDYA010000000
VERSION     DBMDYA010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7528_length_2930_cov_1.394486"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="ACZPFK_08965"
     CDS             complement(<1..971)
                     /locus_tag="ACZPFK_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012700.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR1406483.1"
                     /translation="MLTLGHNLWLYLGTQKKLEGRLETVSYLTAALRCGRSLQKELKG
                     RSPALAAQLGQAAVLGKKLGGGAVLLSPSPQSDAYMLVQVLGMVTLFPILQYQRTARV
                     LEQSQRELCRFFELLGELDASLAVLSYRHSLDAYVQPHFLPGLEVRAKGLRHPLLTET
                     GCVPNDAAIVKNWLLTGSNASGKSTFIKAVALNLILAQNIFTCTAEQFELGRAPVVTS
                     MAARDDVTAGESYFVAEIKSMRRVVARAERGEAFYCFIDEVLKGTNTAERIAASGAVL
                     RYLCAPGCRCVAATHDLELTGLLAAAYENHHFSEQVGPEGVSFNYKL"
     gene            complement(910..1506)
                     /locus_tag="ACZPFK_08970"
     CDS             complement(910..1506)
                     /locus_tag="ACZPFK_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406484.1"
                     /translation="MPAQVVLLLLLAAAMALYLIVDGVRGRKKLLRRLRKNFGAVPDG
                     TYRRASLDHYWREYQAAHPQEESVDDLTWDDLDLEAVFERVNACLTLIGEDYLYALLH
                     RQLGEEALREREKLLGLLEEEPLRLRVQTALARLGKRTGTDLAEVLFHPETFALRSGA
                     AGGAAALSVLPAAGRPMAVAHAGAQPLALSGHPEKIGG"
     gene            complement(1541..>2930)
                     /locus_tag="ACZPFK_08975"
     CDS             complement(1541..>2930)
                     /locus_tag="ACZPFK_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1406485.1"
                     /translation="GQHDAAVAEPVRITGPGADPEPDRTPTSYFTDALMEDVIADLMR
                     ARGVTRAEATRLLYDGGLRVYATVVPSLQSAMEGVMESAGVYPRPGVSVTKPLEDEEG
                     EPVLDAEGNPVTGSVTVRPEAAMVSLNYEGELCAVVGGLGEKEVSRGFNRGTQATRQV
                     GSTMKPIGAYVLAVQNDKITWSTAFEDGPVRQVKDEATGEWKDWPRNFSGTYSDKPIL
                     VADALARSINTIAVRVGEKAGVGNIYRFTKNELGITSFVSADKDSGPMILGSSTTGVT
                     PEQMAGAYSIFGSGGSFTTIHSYTSVQRGSGAVLLEPQVKTEQVVDADTAYIMNRLLA
                     GVMQGQGTGAGYAVNDGMDSVGKTGTTSDNRDHWFIGLTPYYVTASWYGYDDNLPLEV
                     NYSAHPPTLAWRNVMRAAQRGLPQKEFPVDETVEQIDYCAVTGLLPGPGCPRATGYYK
                     QGKGPVGACPGH"
BASE COUNT          456 a         1033 c          939 g          502 t
ORIGIN      
        1 ttgagcttgt aattgaaact gacgccctcc gggcccacct gctcgctgaa atggtggttc
       61 tcataggcgg cggccagcag gccggtcagc tccaggtcgt gggtggcggc gacgcagcgg
      121 caaccgggcg cgcagaggta gcgcagcacc gcgccggagg ccgcgatccg ctcggccgtg
      181 ttggtgcctt tcagcacctc gtcgatgaag caataaaagg cctcaccccg ctccgcccgg
      241 gccaccaccc gccgcatgga cttgatctcg gcgacaaaat agctctcgcc cgcagtcacg
      301 tcgtcccgcg ccgccataga ggtgaccacc ggcgcccgcc ccagctcgaa ctgctccgcc
      361 gtgcaggtaa agatgttttg agccaggatc aggttgaggg cgaccgcctt gataaaggtg
      421 gattttcccg aggcgttgga gccggtgagc agccagtttt tgacgattgc cgcgtcgttg
      481 ggcacgcagc ctgtttcggt gagcagcggg tgccgcaagc ctttggcccg cacctccagc
      541 cccggcagga agtggggctg aacataggcg tccagactgt gccggtaaga gaggacggcg
      601 agagaggcgt ccagctcgcc cagcagctcg aaaaagcggc acagctcccg ctgcgactgc
      661 tccagcaccc gggccgtgcg ctggtactgc agaatcggga acagggtcac catgcccaac
      721 acctgcacca gcatataggc gtccgattgg ggcgaggggc tgagcagcac cgcgccgccg
      781 cccagctttt tgcccagcac cgccgcctgc ccgagctgtg cggccagcgc cgggctgcgc
      841 cctttcagct ctttttgcag gctgcgccca cagcgcagcg ccgccgtcag atagctgacg
      901 gtttccagcc taccctccaa ttttttctgg gtgcccagat aaagccagag gttgtgcccc
      961 agcgtgagca acagccatgg ggcggccagc ggcgggtaga acagaaagag cggcagcgcc
     1021 gccagcggcg ccagagcgga gggcaaacgt ctcggggtgg aaaagcacct ccgcgagatc
     1081 ggtccccgtc cgcttgccca gccgcgccag cgcggtctgc acccgcaggc gcaggggttc
     1141 ctcctccagc aggcccagca gcttctcccg ctcccgcagc gcctcctccc ccaactgccg
     1201 gtgcagcagc gcgtacagat agtcttcgcc gatcaaagtc aggcaggcgt tcacccgctc
     1261 aaagaccgcc tccaggtcca gatcgtccca ggtgaggtcg tcgacgctct cctcctgggg
     1321 atgggcggcc tgatactccc gccagtaatg gtcgagggag gcgcgccggt atgtgccgtc
     1381 cggcacggcg ccaaaatttt tgcgcagccg ccgcagcagt ttttttctgc cgcggacacc
     1441 atccacaatc aggtacagcg ccatggcggc ggcgagcaac aacagcaaaa cgacctgtgc
     1501 cggcatccgc gctcccccct ttctctgccg atccgcccgc tcagtggccg gggcaggcgc
     1561 ccaccggccc cttgccctgc ttgtaatagc ctgtggcgcg ggggcagccc ggccccggca
     1621 gcaggccggt gaccgcgcag tagtcgatct gttccaccgt ttcgtcgacc gggaactctt
     1681 tctgcggcag gccccgctgc gcggctcgca tcacattgcg ccaggccagc gtcggcggat
     1741 gggcggaata gttgacctcc agcggcaggt tgtcgtcgta gccgtaccag ctggcggtga
     1801 catagtaggg ggtgaggccg atgaaccagt ggtcgcggtt gtcgctggtg gtgccggtct
     1861 tgcccaccga gtccatgccg tcgttcaccg catagcccgc gccggtgccc tgcccctgca
     1921 tcaccccggc cagcagccga ttcatgatgt aggcggtatc ggcatccacc acctgctcgg
     1981 tcttcacctg cggctccagc agcaccgcgc cgctgccccg ctggacgctg gtgtagctgt
     2041 ggatggtggt aaagctgccg ccgctgccga agatgctgta ggccccggcc atctgctccg
     2101 gcgtaacgcc ggtggtgctg gagcccagga tcatggggcc gctgtccttg tccgcggaga
     2161 caaagctggt gatgcccagc tcgttttttg tgaagcggta gatgttgccc acccccgcct
     2221 tttcgcccac ccgcaccgcg atggtgttga tgctgcgggc cagcgcgtcc gccaccagga
     2281 tgggcttgtc cgaataggtg ccggaaaaat tgcgcggcca gtccttccac tcgccggtgg
     2341 cctcgtcttt cacctgccgc accgggccgt cctcaaaggc ggtggaccag gtgatcttgt
     2401 cgttttgcac cgccaataca taggcgccga tgggcttcat ggtgctgccc acctgccggg
     2461 tggcctgggt gccccggttg aagccgcggc tgacctcttt ttcgcccagc ccgcccacca
     2521 cggcgcagag ctcgccctca tagttgaggc tgaccatggc cgcctcgggc cgcaccgtga
     2581 ccgacccggt caccgggttg ccctccgcgt ccagcaccgg ctcgccctcc tcgtcctcca
     2641 ggggcttggt cacgctcacc cccgggcgcg gatagacgcc ggcggattcc atcacgccct
     2701 ccatggcgct ctgcagcgag ggcaccaccg tggcgtagac gcgcaggccg ccgtcgtaga
     2761 gcagccgcgt ggcctccgcc cgggtcacgc cgcgcgcgcg catcaggtcc gcgatcacat
     2821 cctccatcag cgcgtcggtg aaatagctgg tgggggtgcg gtccggctcc gggtctgcgc
     2881 ccggcccggt gatgcgcacc ggctccgcca ccgccgcgtc gtgctggcct
//
LOCUS       DBMDYA010000356         2929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7530_length_2929_cov_1.503536, whole genome shotgun sequence.
ACCESSION   DBMDYA010000356 DBMDYA010000000
VERSION     DBMDYA010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7530_length_2929_cov_1.503536"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="ACZPFK_08980"
     CDS             <1..279
                     /locus_tag="ACZPFK_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406486.1"
                     /translation="AIPIFLIPPLFLETSICNFSLSPAVPFFPCPPVDFYFFDAPMRG
                     FGNSVTTIASTEKKQMVFIQNPIYFHKLYLFPYSTVLDYYNTPSPRLQ"
     gene            314..664
                     /locus_tag="ACZPFK_08985"
     CDS             314..664
                     /locus_tag="ACZPFK_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406487.1"
                     /translation="MDLDYKAVGKRGKIARIKADMTQEQLAELVGISPTHLSNIETGT
                     THVSLVTIVRLANALSVTVDDLLCDNVLRAKPQIEQDIAELLDNCDEYEIRMVRDMTR
                     ALLETLRRDANFRS"
     gene            complement(1076..1828)
                     /locus_tag="ACZPFK_08990"
     CDS             complement(1076..1828)
                     /locus_tag="ACZPFK_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406488.1"
                     /translation="MDKKYERGVHLIGRIGLIIGVVFMLGIPAVISAVYHVWPTSITQ
                     VMTIGGGLLLMFIPTALAEVISYTPVLGSSAYITFLTGNVMNLKLPCVINSHTLTGTT
                     QGTDEGDTIAAISVAASSITTTLIIIAGVLLMVPLRPILTSPMVATATSYLLPSLFGG
                     MVLSFMNESCGEYTAKGKNLVMILPLVLVFVINLFYPLSGKEGFAVLACMALNVLIAW
                     VLYKKNIIKMIPKSPAQGKSEMEELAEDMDAE"
     gene            complement(1858..2580)
                     /locus_tag="ACZPFK_08995"
     CDS             complement(1858..2580)
                     /locus_tag="ACZPFK_08995"
                     /inference="COORDINATES: protein motif:HMM:NF027796.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR1406489.1"
                     /translation="MTFLEIMSSPILYILVGLGILYILVFCGITLARSYRHGLAIGLT
                     KAQLRTAITSSSIYSIVPSISIVVGLISLSTVIGVPWAWFRLSVVGAVTYELISAEMT
                     ATGAGFPDMAALGASAEGASLVGTVMFVMSIGILAGVIAILLFGPRIMKGVSGLRSKH
                     GAWGALMTGVLSMAIIEAFLPLQLVKGPVYIAVVITSVVVTVLQLFLIKLTGWKWWNN
                     FIMAVTLLVGMASSLLWTNLLG"
     gene            complement(2628..>2929)
                     /locus_tag="ACZPFK_09000"
     CDS             complement(2628..>2929)
                     /locus_tag="ACZPFK_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1406490.1"
                     /translation="QVAMPGSTDVGDVSYAVPTAQLGSACAAVGTGAHTWQMTAQVGS
                     VLGHKGLLTAGKALALTAARLFEDPSVLERAKQEHKLNAPDGYLCPVPDDVKPSL"
BASE COUNT          658 a          835 c          850 g          586 t
ORIGIN      
        1 gccattccta tttttttgat cccgcctctt tttcttgaaa catccatttg caacttctcc
       61 ctctcgccgg ctgtaccgtt tttcccgtgc ccgcctgtcg atttttattt ctttgacgcc
      121 ccaatgcgtg gattcggcaa ctcggtcaca acgatcgctt caacagaaaa aaagcaaatg
      181 gtttttatac aaaatcccat atatttccat aagctttatt tattcccgta ttctacggtt
      241 ttagattatt ataatacacc ctccccaagg ttacaataaa aaccacagat tattgtcata
      301 aaagagggag taaatggatc tggactacaa agctgtcggc aaaaggggga aaatcgcccg
      361 catcaaagcg gacatgacac aggaacagtt ggcggagctg gtgggcattt cacccaccca
      421 tctgagcaat attgaaaccg ggaccaccca tgtcagtctg gtaaccatcg tgcgtttggc
      481 aaacgcgctc tccgtcactg tggacgacct gctctgcgac aatgtcctgc gggcaaaacc
      541 gcagatcgag caagatatcg cggagctgct ggacaattgt gatgaatatg agatacggat
      601 ggtgcgggat atgacccggg ccctcctgga gacgctgcgc cgggacgcca atttccggtc
      661 atgaccgctg cacaaataaa ccgggcgggg caagtagata aaacttgccc cgcccaagct
      721 attttatggc cgttttcagc agcgtttcgc tccggcgcgg ccggctgtga agcacgcagc
      781 tgtcgggatg agcccgcctc ccggcgcggg acagagcgcc gaagaaaaag cggcttgtct
      841 cgatcgtccc gtttctcgcc gctgtcgcgg caaccgaaac ggatggccga aaaaaaatag
      901 ccgcaaagcg gctatttttc ttgttgttcc gcaacaagaa aactttttat cttgatggcc
      961 cgtttctcgc cgctgtcgcg gcgaccgaaa cggatggcca aaaaaaatag ccgcaaagcg
     1021 gctatttttt tatcatccag cagatctcaa aatgggcaac aggggcggga cgtggtcact
     1081 ccgcgtccat atcctccgcc agctcctcca tttcgctctt gccctgggcg ggactcttgg
     1141 ggatcatctt gatgatgttc tttttgtaca gcacccaggc gatgagcacg ttcagcgcca
     1201 tgcaggccag caccgcaaag ccctctttgc cggacagcgg gtagaacagg ttgatcacaa
     1261 ataccagcac caggggcaga atcatgacca ggtttttgcc cttggcggtg tattcgccgc
     1321 agctctcgtt cataaagctc agcaccatgc cgccgaacag ggagggcagc agatagctgg
     1381 tggcggtggc aaccatcggg ctggtcagaa tcgggcgcag aggcaccatg agcaggacac
     1441 ctgcgataat gatcagggtg gtagtgatgg aagacgcagc cacgctgatg gcggcgatgg
     1501 tgtcgccctc gtcggtgccc tgggtggtgc cggtcagggt gtgtgagttg atgacgcagg
     1561 gcaatttcag gttcatgacg ttaccggtca ggaacgtgat gtaggctgag gagcccagca
     1621 ccggggtgta gctaataacc tccgccaggg cggtggggat gaacatcaac agcagaccgc
     1681 cgccgatggt catcacctgg gtgatcgagg tgggccagac atgatagacc gcgctgatga
     1741 ctgccgggat gcccagcatg aataccacac cgatgatcag cccgatgcgg ccgatcaggt
     1801 gaacgcctct ctcgtacttt ttatccatgt tcgtttcatc ctttctctag tgaggtctca
     1861 gcccaggagg ttggtccaga gcagtgaaga ggccataccg acaagaagcg tgacggccat
     1921 aataaagttg ttccaccact tccagccggt cagcttgatc aagaacagct gcagcaccgt
     1981 gacgacgacc gaggtgatca cgaccgcgat gtagaccggg cccttgacca actgcagggg
     2041 cagaaacgct tcgataatgg ccatcgacag cacgccggtc atcagcgcgc cccaggcccc
     2101 gtgcttgctg cgcaggccgg agaccccttt catgatgcgg ggtccaaaga gcaggatggc
     2161 gatgacgccg gccaggatgc cgatgctcat gacgaacatg acagtgccca ccagagaggc
     2221 gccctccgcg gaagcgccca gcgcggccat atccggaaaa ccggcgccgg tggcggtcat
     2281 ctccgccgag atcagctcat aggtgacggc gcccacgacg gacaggcgga accaggccca
     2341 gggcacgccg atcacggtgg acaggctgat caggccgacc acgatggaga tggagggaac
     2401 gatggagtag atggaagacg aggtgatggc ggtcctcaac tgagccttgg tgaggccgat
     2461 cgccagcccg tgcctgtagg agcgggccag ggtgataccg cagaaaacca ggatgtacag
     2521 gataccgagg cctaccagaa tgtacaggat cgggctggac atgatttcca gaaaggtcat
     2581 gatttgattt cctccttcat taccggacaa tttttttcaa gcccatctca caggcttggc
     2641 ttgacgtcgt cgggcaccgg gcagagataa ccgtccggcg cgttgagctt gtgctcctgc
     2701 ttggcgcgct cgagcaccga gggatcctcg aacagccggg ccgcggtgag ggccagcgcc
     2761 ttgcccgcgg tcagcaggcc cttgtggccg agaacgctgc ccacctgggc cgtcatctgc
     2821 caggtgtgtg cgccggtgcc gaccgccgca caggccgacc ccagctgagc cgtgggcacc
     2881 gcatagctca catcgcccac atcggtgctg ccgggcatgg ccacctggg
//
LOCUS       DBMDYA010000357         2922 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7549_length_2922_cov_1.649770, whole genome shotgun sequence.
ACCESSION   DBMDYA010000357 DBMDYA010000000
VERSION     DBMDYA010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7549_length_2922_cov_1.649770"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="ACZPFK_09005"
     CDS             complement(<1..495)
                     /locus_tag="ACZPFK_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406491.1"
                     /translation="MKDKLKAVLKYPAVLLFFAFILFYSLADSMVNNRRESELENRKL
                     AQKPAMTVKNLLATKETEKFSYLYEQYINDQFLGRDNWISLKSRAESVLLKTENNGVL
                     YGKEGTMYQKFFTLDEAAGGGTQLEVNIENVRRFVARHPGLVKVMIVPSADMVTTDKL
                     PLAAP"
     gene            complement(511..1899)
                     /locus_tag="ACZPFK_09010"
     CDS             complement(511..1899)
                     /locus_tag="ACZPFK_09010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1406492.1"
                     /translation="MVFSSLLFIFRFLPIALAVYYLAPPRLKNLALFLISLFFYSWGE
                     VRYFPVMAACILGNYLLALWLGRLRDKPRARRAVLVLVVVFNIGMLFFFKYTDFFITN
                     LNALLGTSIPLLRLTLPLGISFYTFQTMSYTLDVYKGTVQPERNIIDLGAYVVMFPQL
                     IAGPIVLYKDVAAQLKVKKGRYTLAGINEGVGLFILGLGKKVLLANNIGALWEDIKLL
                     GYGSVSTPLAWLGLVAFTLQIYFDFSGYSTMAIGLGKMLGFNFPENFNLPYISRSITE
                     FWRRWHMTLSGWFREYVYIPLGGNRRGLPRQIFNLFVVWTLTGFWHGANWNFLLWGFY
                     YFVLLAVEKLFLYDKLQRGRVWPHLYALFFIVVGWGLFAVDSGMADIVAFFSRLFWPQ
                     GGVSALYFARNYGVALLVGMFCSTPWPRRFYAWVCKKSELLRILVLAAVLLVSVAYLV
                     DSTYNPFIYFRF"
     gene            complement(2039..2542)
                     /locus_tag="ACZPFK_09015"
     CDS             complement(2039..2542)
                     /locus_tag="ACZPFK_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025628.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1406493.1"
                     /translation="MKKICSLLLALCTLAALATGCGKDAETKQYNLGDVMDAIEAVAP
                     VAMPADMDDEFLTSMYGLNMEDVEEYKGKYSNVNISTDEILIIKTAKGKTDAVKEACE
                     ARQKAKADQFAMYLEDQALKAQNGRIVVKGDYVIFVIAGDSTRIADGEVDAVYQEIDK
                     AIDDALA"
     gene            complement(2638..>2922)
                     /locus_tag="ACZPFK_09020"
     CDS             complement(2638..>2922)
                     /locus_tag="ACZPFK_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025628.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1406494.1"
                     /translation="YANTNLSTDEILVLKAVEGKADALGDACEARRAAKQEQAAMYDE
                     VQAQKAENGRIVVKGDYVIFVVAGDDGRIADGEVNEVYQEIDGAIEKALA"
BASE COUNT          635 a          863 c          855 g          569 t
ORIGIN      
        1 gggggccgcc agcggcagtt tgtcggtggt caccatatcg gcggagggca cgatcatcac
       61 cttgaccagt cccgggtggc gggcgacgaa ccggcgcaca ttttcaatgt tgacctccag
      121 ctgggtgccg cctccggccg cctcgtccag ggtgaagaac ttctggtaca tggtgccctc
      181 tttgccgtag agcacgccgt tgttttctgt tttgagcagc acgctctccg cccggctctt
      241 caggctgatc cagttgtccc ggcccaaaaa ctggtcgttg atgtactgct cgtacaggta
      301 agagaacttt tccgtctcct tggtggcgag cagatttttg accgtcatgg cgggtttttg
      361 ggccagcttg cggttttcca gctcgctctc cctgcggttg ttgaccatgc tgtccgccag
      421 ggaataaaac aggataaagg cgaaaaagag caaaacggcc ggatatttca ggacggcctt
      481 gagtttgtct ttcatttcac tgcctcccct ctaaaaccgg aaatagatga acgggttata
      541 ggtgctgtcc accagatagg ccaccgagac cagcagcacc gcagccagca ccaaaatgcg
      601 cagcagctcg ctctttttgc agacccaggc gtagaatctg cgcggccagg gggtgctgca
      661 gaacatgccc accagcagcg ccacgccgta gttgcgggcg aaatagagcg cggagacgcc
      721 gccctggggc cagaacagcc gcgagaagaa agcgacgata tccgccatgc cactgtccac
      781 ggcaaaaagc ccccagccca ccacgatgaa gaacagggcg tagaggtgcg gccagacccg
      841 gccgcgctgc agcttgtcgt aaagaaacag cttttccacg gccagcagca caaaatagta
      901 aaagccccac aacaaaaagt tccagttggc gccgtgccaa aaaccggtga gggtccacac
      961 cacaaagagg ttgaagatct ggcgcggcag cccgcggcgg ttgccgccca gcggaatata
     1021 gacgtattcg cggaaccagc ccgacagggt catatgccag cgccgccaga attcggtgat
     1081 actgcggctg atatagggca gattgaagtt ttcagggaaa ttgaagccca gcattttgcc
     1141 aaggccgatg gccatggtgg aatagccgga aaaatcaaaa tagatctgca gcgtgaaagc
     1201 caccagaccc agccaggcca ggggggtgga cacgctgccg tagcccagca gcttgatatc
     1261 ctcccacagg gcgccgatgt tgttggccag cagcactttt ttgcccagcc ccaggatgaa
     1321 caggcccacg ccctcgttga tgcccgccag cgtatagcgg ccctttttca ccttgagctg
     1381 tgcggccaca tctttataga gcacgatggg cccggcgatc agctgcggga acatcaccac
     1441 ataagcgccc aggtcgatga tattgcgctc gggctgcacc gtgcccttgt acacgtccag
     1501 cgtataactc atggtctgga acgtgtaaaa gctgatgccc agcggcaggg tcagccgcag
     1561 cagggggatg gaggtgccca gcagcgcgtt gagattggtg atgaagaaat cggtgtattt
     1621 gaagaaaaac agcatgccga tgttgaagac gaccaccaac accagcacgg cgcgccgggc
     1681 gcggggcttg tcccgcaggc ggccgagcca gagcgccagc agatagttgc ccaggatgca
     1741 ggccgccatg acggggaaat agcgcacctc cccccaggaa taaaagaaaa ggctgatcag
     1801 gaacagggcc agatttttga gccggggagg cgccagataa tagactgcca gcgcgatggg
     1861 cagaaaacgg aagatgaaca gcagactgct gaagaccaaa acacatcacc aaccctgcgg
     1921 gcggctcgcc gcccggatat ttgcaaaaga aaaaccaaag gggccggcgc ctccatgacg
     1981 ccggccccgg attcagtata aaggaaaaac aagcgccgca caagcggttt ttgagaaatc
     2041 aggccagcgc gtcgtcgatc gccttgtcga tctcctggta aaccgcgtcc acctcgccgt
     2101 ccgcgatgcg ggtcgagtcg ccggcgatga caaagatcac gtagtcgccc ttgacgacaa
     2161 tacgaccgtt ctgcgccttg agagcctgat cctccaggta catggcgaac tgatcggcct
     2221 tggctttctg gcgggcctca caggcctcct tgacggcgtc ggtcttgccc ttggcggtct
     2281 tgatgatcag gatctcgtcg gtggagatgt tgacgttgga gtatttgccc ttatattctt
     2341 ccacgtcctc catattgagg ccgtacatgc tggtgagaaa ttcatcgtcc atatcagcgg
     2401 gcatggccac aggggccacg gcctcgatcg cgtccatcac gtcgcccagg ttgtactgct
     2461 tggtttcggc gtccttgccg cagccggtgg cgagcgccgc cagggtgcag agcgccagca
     2521 gaagagaaca gatttttttc atattgccaa aaagtccttt ccgatttttt tcattccgca
     2581 actctgtctt tatggtaaaa gcgcaggggc cggggccgcc cgcgttttta cgaccgttca
     2641 ggccagggcc ttttcaatcg ccccgtcgat ctcctgatag acctcgttca cctcgccgtc
     2701 cgcgatgcgc ccgtcgtccc cggcgaccac gaagatcacg taatcgcctt tgaccacaat
     2761 gcggccgttt tcggctttct gcgcctgcac ctcatcgtac atggcggctt gctcctgctt
     2821 tgcggcccgg cgggcctcac aggcgtcccc gagggcgtcg gccttgccct cgacggcctt
     2881 gaggaccagg atctcgtcgg tggagaggtt ggtgttggcg ta
//
LOCUS       DBMDYA010000358         2921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7552_length_2921_cov_1.587234, whole genome shotgun sequence.
ACCESSION   DBMDYA010000358 DBMDYA010000000
VERSION     DBMDYA010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7552_length_2921_cov_1.587234"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="ACZPFK_09025"
     CDS             complement(<1..930)
                     /locus_tag="ACZPFK_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1406495.1"
                     /translation="MEYTVSKKHRDAVAALTGDEKVKKALEFLKEDHERCVREQVTIT
                     QIASPTFHEEKRAAYMTEQFRALGLTDVHIDAIGNAVGVRKGKGTGPKVVVDGHMDTV
                     YAEDTPLEPTFDGEFIHCPGIVDDTRALAAMLSLIRALDAAGIETDGDLVFTGTVQEE
                     GMGAFSGARAFYEENRDIGAGIHMDGCGAAGIIYQSTGIKTLEITFHGVGGHAYMAFG
                     EIANPLHAAARAVAKIADLQVPEYPKTTFAVSNFHAGTDAAIHAIVPEATIKINYRSN
                     GAAELEALDKEVFRCIEEACKEETERWGKDVITY"
     gene            complement(994..1929)
                     /locus_tag="ACZPFK_09030"
     CDS             complement(994..1929)
                     /locus_tag="ACZPFK_09030"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1406496.1"
                     /translation="MWETSIFGFTLYQLFWYFVLYSLLGWCAEVVFCTVNTGKFVNRG
                     FLNGPVCPIYGFGMLIVLCALTPLSGSLPVLFVGGAVLASLLELVTGFALEKLFHTQW
                     WDYSDKPFNVGGYICLSFSLLWGLLIVLVMRVIHPVVAGAVAVVPTLAGEIAAVPVLL
                     AYLADLAVTVRGILKFNRSLGELEKIAQALHKVSQGLSETLGEKSLEAAAAVGEKGQA
                     LGEKLEETKANLSGLLDGTRASLDEKRLQAALGAAEYRDELQRRLDELQARYNELMAA
                     RPSRISRRLMRAFPDMKLRRGGEILDELRERLKRK"
     gene            complement(1976..2389)
                     /locus_tag="ACZPFK_09035"
     CDS             complement(1976..2389)
                     /locus_tag="ACZPFK_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1406497.1"
                     /translation="MDSSVKRFLTEMADRASAMAEGAMDAVAGAGKVVGEKKDAAMLR
                     LDLLRLRSECESRFSEIGRTFYLMNAGAWPKEGETAEHRIESLLGQVGEREMQISDIL
                     RRLAELSGKSACPACGKPCEAGAAFCPACGKPLKG"
     gene            2595..>2921
                     /locus_tag="ACZPFK_09040"
     CDS             2595..>2921
                     /locus_tag="ACZPFK_09040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1406498.1"
                     /translation="MDRVILHCDCNSFYASVELLEHPELRNRPVAVGRDDGSRHGVVL
                     AKNEAAKKFGVKTAETIWQARRKCPELVLLPPHHEKYRAMSARINRIYGEYTELVEPF
                     SVDESWL"
BASE COUNT          497 a          976 c          926 g          522 t
ORIGIN      
        1 gtaggtgatg acgtccttgc cccagcgctc ggtctcctcc ttgcaggcct cctcgatgca
       61 gcggaagacc tccttgtcca gggcctccag ctcggccgcg ccgttggagc ggtagttgat
      121 cttgatggtg gcctcgggca cgatggcgtg gatcgccgcg tcggtgccgg cgtggaagtt
      181 ggataccgca aaggtggtct tggggtactc cggcacctgc aggtcggcga tcttcgccac
      241 cgcgcgcgcc gcagcgtgca gcgggttcgc aatctcgccg aacgccatgt aggcgtgccc
      301 gcccacgccg tggaacgtga tctccagcgt cttgatgccg gtggactggt agatgatgcc
      361 cgccgcgccg cagccgtcca tgtggatgcc cgcgccgatg tccctgttct cctcgtaaaa
      421 agcgcgcgcg ccgctgaaag cgcccatgcc ctcttcctgc acggtgccgg tgaagaccag
      481 gtccccgtcg gtctcgatgc ccgccgcgtc cagcgcgcgg atcagcgaga gcatggcggc
      541 cagggcgcgg gtgtcgtcca cgatgccggg gcagtggatg aactcgccgt caaaggtggg
      601 ctccagcggg gtatcctcgg cgtagacggt gtccatgtgg ccgtccacga ccactttcgg
      661 cccggtgccc ttgcccttgc gcacccccac cgcgttgccg atggcgtcga tgtgcacgtc
      721 ggtgaggccc agcgcgcgga attgctcggt catgtaggcg gcccgcttct cctcgtgaaa
      781 ggtgggggag gcgatctggg tgatggtcac ctgctcgcgg acgcagcgct catgatcctc
      841 tttgaggaat tccagcgcct ttttcacctt ttcgtcgccg gtcagggcgg cgacggcgtc
      901 gcgatgcttt ttacttaccg tatactccat attgttctct ccttaaagtt attccgtcgg
      961 cgggagcggc gctccgcctc ccccgccgga cggtcatttc cgtttgaggc gctcgcgcag
     1021 ctcgtcgagg atctcgccgc cgcgccggag cttcatatcc gggaacgccc gcatcagccg
     1081 ccggctgatg cggctgggcc gcgcggccat gagctcgtta tagcgggcct gcagctcgtc
     1141 cagcctgcgc tgcagctcgt cgcggtattc cgccgcgccc agcgccgcct gcagccgctt
     1201 ctcgtccagc gaggcgcggg tcccgtccag taggccgctc aggttcgcct tggtctcctc
     1261 cagcttttcg cccaaagcct ggcccttttc gcccaccgcc gccgcggcct ccagcgattt
     1321 ttcacccagc gtctcggaca ggccctggga caccttatgc agggcctggg cgatcttttc
     1381 cagctcgccc aggctgcggt tgaatttcag gatgccgcgc accgtgaccg ccagatcggc
     1441 cagataggcc aacagcaccg gcacggccgc aatctcgccc gccagcgtgg gcaccaccgc
     1501 caccgcgccc gccaccaccg ggtggatcac ccgcatcacc agcacgatca gcagccccca
     1561 cagcagcgaa aacgagaggc agatatagcc gcccacattg aacggcttgt cgctgtaatc
     1621 ccaccactgg gtgtggaaca gcttttccag cgcaaagccg gtgaccagct ccagcaggct
     1681 ggccagcacc gcgccgccca caaagagcac cggcaggctg ccggagagcg gtgtgagcgc
     1741 gcagagcacg atcaacatgc cgaagccgta gatggggcag accgggccgt tgagaaagcc
     1801 gcggttgacg aatttgccgg tgttgacggt gcaaaaaacc acctcggcgc accagcccag
     1861 cagcgaatag agcacaaaat accagaagag ctggtagaga gtgaatccaa aaatcgacgt
     1921 ctcccacata agggggcagg gctcctttct ttttttgccg ccgcggcgcg cgggcctagc
     1981 ccttcagcgg tttgccgcag gcggggcaaa aggcggcccc ggcctcgcag ggcttgccgc
     2041 aggcggggca ggcgcttttg ccggacagct ccgccagacg gcgcaggata tcggagatct
     2101 gcatctcccg ctcccccacc tgccccagca gcgattcgat gcggtgctcg gccgtctcgc
     2161 cctctttggg ccaggcgccc gcgttcatca gataaaaggt gcggccgatc tcgctgaagc
     2221 gggactcgca ctcgctgcgc agccgtagca ggtccaggcg cagcatcgcc gcgtcctttt
     2281 tctcccccac caccttgccg gcgcccgcga cggcgtccat tgcgccctcc gccatggcgg
     2341 aggcgcggtc ggccatctcg gtcaaaaagc gtttcacgct ggaatccatg cagaaaacct
     2401 cccgtccaaa tttgtttttt tccgttgaac atttctagta taccatcttt ggcggggctt
     2461 tgcaccccac aaaaagcggc ggttgtccgc cccggccaaa atgagtataa taggagaaaa
     2521 agaaagacgg caaatgccgg ggcccgacag ggagaggaca ccgtgttcgg actcggctga
     2581 ggggggcgag gcggttggac cgggtgatcc tgcactgtga ctgcaattct ttttacgcct
     2641 cggtggagct gctggagcac cccgagctgc gaaacaggcc ggtggcggtg gggcgggacg
     2701 acggcagccg ccacggggtg gttctggcca agaacgaggc ggcaaaaaag tttggcgtca
     2761 agaccgccga gaccatctgg caggctaggc gcaagtgccc cgagctggtg ctgctgccgc
     2821 cccaccacga aaaataccgc gccatgagcg cgcgcatcaa ccgcatctac ggcgagtaca
     2881 ccgagctggt ggagccgttc agcgtggatg agagctggct g
//
LOCUS       DBMDYA010000359         2912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7574_length_2912_cov_1.485592, whole genome shotgun sequence.
ACCESSION   DBMDYA010000359 DBMDYA010000000
VERSION     DBMDYA010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7574_length_2912_cov_1.485592"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            785..1657
                     /locus_tag="ACZPFK_09045"
     CDS             785..1657
                     /locus_tag="ACZPFK_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013625.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1406499.1"
                     /translation="MSELERAGLSALAPAFAADPRPTVDPPYPGRLLRLGSSGPEVAL
                     LQRRLEGIRTGRYPELAPLSPDGVFGQRTRTAVQQYQSLAGLAVDGIVGRDSWASIVT
                     TYNTLYPDGDGRWPGGTLHPGSRGPEVVRMQQKLNYLANLYTAINRQAVDGAYGPKME
                     AAVRRFQAQFGLEADGAVGRATWERITAVQANAERGEPDGVLPRYPGRPLRRGDRGDN
                     VRFLQSYLNEVLHAEDPGVAQVRVDGSFGPATEALVRGFQRHFRLAEDGAAGRDTWGV
                     AVAEFNRLVMRGRG"
     gene            complement(1776..2009)
                     /locus_tag="ACZPFK_09050"
     CDS             complement(1776..2009)
                     /locus_tag="ACZPFK_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406500.1"
                     /translation="MTDFMNAAFPFVVISICLAIISANHHKQEGKEDAKNYMVEGMCL
                     GVVFSDSLGIGLGLGASLGMLIGKTAGMFSKKK"
     gene            2291..>2912
                     /locus_tag="ACZPFK_09055"
     CDS             2291..>2912
                     /locus_tag="ACZPFK_09055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR1406501.1"
                     /translation="MSKIIRDLSPLNAREDMPTVVYVLKKLIAFFLIYFVSMVAAEGI
                     VVLLHYFLGYDVLHGEMMGAQQMTLMKYYGYLVFLAVGLLYCRVVEKRSPETLGFNKN
                     FFDYFIGMAIALALVSASLAVLLSLGAVRAGGGSLHPDGGLITAFFFGFVIQGAMEET
                     LCRGFLMTSLQKKGNTALAVLVSSLAFVLPHLPSLFGEGLVYGLIGT"
BASE COUNT          439 a          941 c          999 g          533 t
ORIGIN      
        1 cttttctgtg gcggccaaag ccgttgcggg ccgccggaaa gcgctttgca gaggtttcgg
       61 cgctcgccca cctgcggcgt gtaacaaaga agcgtaagat ggtcacggcc caggtcggag
      121 ttcgcccccc acccgcggcc gatgctgtag ctttccatgc cctcgggcgg acagcccgag
      181 ccagcgcgtt tgtctgccgg gatcgggatc agccgcgcgc gcccctatgg aggcggcccg
      241 gcctgttttt ttgttgccgg tctccgcgga gcggggcgcg ccggtgagag aaagggcggg
      301 gcgggcccgg cgcggacagg ggcccgcgag gccttttcgc accgcgcagg ctttgccgcg
      361 gcagcccgca gggtcggtca gggcggattt cctgggcagg gatggcgagg tctgacgggc
      421 agggcggtca gcatggccgt gtgtctgcgc ggtcttttcc cacgccgcgc cggctttgca
      481 gcggtggctg tgtggatttg ggccagcggg caaaaacggt cttttcccgc gccgcgcagg
      541 ctttgccgcg gccacccgtt tccgcgtgca gatgattccg cattattccg gtccggcgcg
      601 gcctatcgga ctcccgcagc ttccctttcc cctttgcgcc caccccccag cgcgagcagg
      661 ccccgtcacc ccgcccggcc cgcgcgcata ggatggcggg atggggagag gggcgcgcgg
      721 cggctccgcc ggggctgccg cgcgcgattc tgcgttcggc cctccccaaa aggaggagaa
      781 cgaaatgtcg gaactggaac gcgccggcct gtccgccctg gcgccggcct ttgcggccga
      841 tccgcgcccc acggtggacc cgccttatcc cggccgcctg ctgcgcctgg gctcgtccgg
      901 gccggaggtg gcgctgctgc agcggcgcct ggagggcatc cgcaccgggc gctatcccga
      961 gctggcgccc ctctcgccgg acggcgtctt tgggcagaga acccgcaccg cggtgcagca
     1021 ataccaatcg ctggcgggcc ttgccgtcga cggcattgtg ggccgggaca gctgggcctc
     1081 catcgtcacc acctacaaca ccctctaccc cgatggggac ggccgctggc ccggcggcac
     1141 gctgcacccg ggcagccgtg ggccggaggt ggtgcgcatg cagcagaagc tgaactacct
     1201 ggcgaacctc tacaccgcca tcaacaggca ggcggtggac ggggcctacg gccccaaaat
     1261 ggaggcagcg gtgcgccgct ttcaggccca gttcgggctg gaggcggacg gcgcggtggg
     1321 ccgcgccacc tgggagcgca tcacagccgt gcaggcgaac gccgaacggg gcgagccgga
     1381 cggcgtgctg ccccgctatc ccggccgccc gctgcggcgc ggcgaccggg gcgacaacgt
     1441 gcgctttttg cagagctacc tgaacgaggt actgcacgcc gaggacccgg gcgttgcaca
     1501 ggtgcgggtg gacggcagct tcggcccggc caccgaggcg ctggtgcggg gctttcagcg
     1561 gcacttccgc ctggcggagg acggcgcggc cggccgggac acctggggcg tggcggtggc
     1621 cgagttcaac cggctggtga tgcgcggccg tggataaagc gccgcccgcc ctgccgctgc
     1681 ctgcggcgcc ctgcggcccg ctgccgcgaa agaagaagcg ctccccggca acggcggccc
     1741 tcgcggccgc cgtttttggc gtccgcctgt tttgcctatt tctttttaga gaacatgccc
     1801 gccgtcttgc cgatcagcat gcccaggctg gcgcccaggc ccaggccgat cccgagcgaa
     1861 tcggaaaaca cgacccccag gcacatgccc tcgaccatat aattcttggc gtcctctttt
     1921 ccctcttgct tgtggtgatt ggcagaaata atcgccaggc agatactgat aacgacaaag
     1981 gggaaggcgg cgttcataaa atctgtcatg aaaaaaacct ctctttcaga tttggtcggg
     2041 cgcgcagggc gctttttgtc cctgttgcgg gctgcttgcc cgctgactca cgcccggatg
     2101 agaccattat acgcccgcgg caattcaacg gcccggtgcg gcggggctgc cccgacccgc
     2161 ttttgcggcg gcccggcccg cgaagccggc gggcgcgggg cccggccgga aaagagggat
     2221 ttccaccccc ctgcggttgt ggtagaataa aagaacaatg cggcgcatca agctgcgagg
     2281 aggcgggacg atgtcaaaga tcatccggga cctgagccct ttgaacgcgc gggaggatat
     2341 gcccacggtc gtgtatgtcc tcaaaaaact gatcgccttt ttccttatct atttcgtgtc
     2401 catggtggcg gcggaaggga tcgtcgttct tctgcactat ttcctgggat acgatgttct
     2461 gcacggcgaa atgatgggcg cgcagcagat gaccctgatg aaatattatg ggtatctcgt
     2521 ctttttggcg gttgggctgc tctactgcag ggtcgtcgaa aagcgctccc ctgagacact
     2581 ggggttcaac aaaaattttt ttgattattt tatcggcatg gcgattgcgc tggcgctggt
     2641 gtcggcaagc ctcgcggtgc tcctgtcgct cggggcggtc agggcgggcg gcggatcgct
     2701 tcaccccgac ggcggcctga tcaccgcttt tttctttggg tttgtcatcc agggcgccat
     2761 ggaggagacg ctgtgcaggg gctttttgat gacgtcgctt caaaaaaagg gtaacacggc
     2821 gctggcggtg ctggtcagct ccctcgcctt tgtgctgccg catctgccct ccctgtttgg
     2881 cgagggcctg gtatacggcc tgatcgggac ga
//
LOCUS       DBMDYA010000360         2903 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7593_length_2903_cov_1.255889, whole genome shotgun sequence.
ACCESSION   DBMDYA010000360 DBMDYA010000000
VERSION     DBMDYA010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_2903_cov_1.255889"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="ACZPFK_09060"
     CDS             <1..381
                     /locus_tag="ACZPFK_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1406502.1"
                     /translation="FDMRELLARVRALARRPGALAAPDLLCYADLSLDRGSLALRGGK
                     GGCVLSKKEAEVLAALLQSGGRTLSRTLLFGRVWGADTEVEEASLDSYIHFVRRRLAA
                     VSERVRVATVRGVGYRLEDVQNAT"
     gene            371..1708
                     /locus_tag="ACZPFK_09065"
     CDS             371..1708
                     /locus_tag="ACZPFK_09065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1406503.1"
                     /translation="MRHEGHDGAAGRGAPVLLKPLRRRLTLIFTALTGSVLIAMLLLT
                     LMLAQQQARTAARGRLTDIANTLIEQLRSGGTVSDDWLARTEAASRLVIGIADRGHPL
                     FFSGSWPPATDRETLLERARQEAEALGLPLGEAPASSLYAARQQFELVGDSGDRYLAE
                     AALIPADGGWYSLILLQDTRPLQSVLLRLRLLYGGIGAAGVVLLLAVSWFLSGRVLRP
                     TAESIQKQRDFVAAASHELRSPLAVIRTSLSAIPADPTREARYLKNIEGEARRMSRLV
                     DDLLILAGSDAKSWKLRMEPVDLDALLIDAFDQFEPLARAKGIALRPLLPEAPVGAAD
                     GDRGRLMQVLSVLLSNAADYAPPGSAVTLEARRGKTRALITVSDQGPGVPDREKERIF
                     DRFYRADAARSGKSHFGPGLSVARELAALHGGTLTVRDAPGGGAAFTLSLPLR"
     gene            1902..2465
                     /locus_tag="ACZPFK_09070"
     CDS             1902..2465
                     /locus_tag="ACZPFK_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR1406504.1"
                     /translation="MKKILNEYAYSALAGVVLGLVLLIWPSLSGKLLCYGLALAFLAY
                     GIYRIVCYFIRDAVLTMLRRDLAVGLVTIAVGIFIFARPELLVSLLPVLFGLLLVVGA
                     MSEVQTAFDMKRMEDGRWYFSLIAAVVLAVLGLVILANPFSTALVLMRFTGAALVVEG
                     VCEFVFSILLTKRQRLYYPPEDDGDSF"
     gene            complement(2697..>2903)
                     /locus_tag="ACZPFK_09075"
     CDS             complement(2697..>2903)
                     /locus_tag="ACZPFK_09075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR1406505.1"
                     /translation="LAPGQGDGALLQRIAALAEKGVVFAACRNALRAQRLEPKALAQF
                     AVTVPAGVVELAQKQQAGYAYIRP"
BASE COUNT          409 a          983 c          992 g          519 t
ORIGIN      
        1 tttgacatgc gggagctact ggcccgggtg cgggcgctgg cgcgcaggcc cggcgccctg
       61 gctgcgcccg acctgctctg ctatgccgac ctctcgctgg accgggggtc gctggcgctg
      121 cgcggcggca agggcggctg cgtgctgtca aaaaaagagg ccgaggtgtt ggccgccctg
      181 ctgcagagcg gcgggcgcac cctgtcccgg acgctgctgt tcggccgcgt gtggggcgcc
      241 gacacagagg tggaggaggc cagcctggac agctatatcc actttgtgcg ccgccgtctt
      301 gccgccgtga gcgagcgggt gcgggtggcg acggtgcggg gcgtggggta ccggctggag
      361 gatgtgcaaa atgcgacatg agggccatga cggggcggcg gggcggggcg cgcccgtcct
      421 gctgaagccc ctgcgccgcc gcctgaccct gatcttcacc gccctcaccg gctcggtgct
      481 catcgccatg ctgctgctca cgttgatgct ggcgcagcag caggcgcgca ccgccgcccg
      541 cgggcggctg accgacatcg caaacaccct gatcgaacag ctgcgctcag gcgggacggt
      601 cagcgacgac tggctggcgc gcacggaggc ggcctcacgg ctggtcatcg gcattgccga
      661 ccggggccac ccgctctttt tctccggctc ctggccaccc gccaccgacc gggaaacgct
      721 gctggagcgg gcgcggcaag aggcggaagc tctgggcctg cccctggggg aagcgcccgc
      781 ctcctcgctg tacgccgccc ggcagcaatt cgagctcgtc ggggacagcg gggaccgcta
      841 tctcgccgag gccgcgctca tccccgccga cgggggctgg tacagcctga tcctgctgca
      901 ggacacccgg cctttgcaga gtgttctgct gcgcctgcgg ctgctctatg ggggcatcgg
      961 tgcggcgggg gtcgtgctgc tgttggcggt gagctggttt ttgtccggcc gggtgctgcg
     1021 ccccaccgcc gaatccatcc aaaagcagcg cgactttgtc gccgccgcca gccacgagct
     1081 gcgcagcccc ctggccgtga tccgcaccag cctctcggcc atcccggccg acccgacgcg
     1141 ggaagcgcgc tatctcaaaa atatcgaggg cgaggcacgg cggatgtccc gtctggtgga
     1201 cgacctgctc atcctggcgg gcagcgacgc gaagagctgg aagctgcgca tggagccggt
     1261 ggacctggac gcgctgctca tcgacgcgtt cgaccagttt gagcccctgg cgcgggcgaa
     1321 aggcatcgcc ctgcgccccc tgctgccaga ggccccggtg ggcgccgccg acggggaccg
     1381 gggccgcctg atgcaggtgc tctcggtgct gctgtccaat gcggcggact atgcgccgcc
     1441 gggcagcgcc gtgacgctgg aggcccggcg cggcaaaaca cgcgccctta tcaccgtgtc
     1501 ggaccagggc cccggtgtgc ccgaccggga aaaggagcgc atctttgacc gcttttaccg
     1561 cgccgacgcc gcccgcagcg gcaagagcca ctttggcccg gggctgtcgg tggcccgcga
     1621 gctggccgcc ctgcacgggg gcacgctgac ggtgcgggac gccccgggcg gaggcgccgc
     1681 tttcaccctg tcgctgccgc tgcgttgaac ccggcagcac cgcaaagcgc ttttttcggg
     1741 gacgctgcgg ccccgcggac aaaaaaatcg ctcctttttg acaattcccg cgttttgcgt
     1801 cttccccttt gcggtctgct gcggtatgat taggactgaa cgatcctgcc aaaaacaaaa
     1861 gcgtcccggc attgccggga cgaaaggggt tgccgtcacc gatgaaaaag atcctcaatg
     1921 aatacgccta ttccgccctg gcgggtgtgg tgctgggcct ggtgctgctg atctggccct
     1981 cgctctcggg caagctgctc tgctacgggc tggcgctggc ctttttggcc tacggcatct
     2041 accgcatcgt ctgctacttc atccgggacg cggtgctcac catgctgcgc cgcgatctgg
     2101 cggtgggcct tgtcaccatc gccgtgggca ttttcatctt tgcccggccg gagctgctgg
     2161 tctcgctgct gccggtgctg ttcggcctgc tgctggtggt gggggcgatg agcgaggtgc
     2221 agaccgcctt tgacatgaag cggatggagg acggccgctg gtatttttca ctcatcgccg
     2281 ccgtggtgct ggcggtgctg gggcttgtca tcctggcgaa tcccttttcc acggcgctgg
     2341 tgctgatgcg ctttaccggc gcggcgctgg tggtggaggg cgtctgtgaa ttcgtctttt
     2401 ccatcctgct caccaagcgc cagcggcttt attatccccc ggaggacgac ggggacagct
     2461 tttaaaagcc cgggtccaaa agcaccggcg ccaaggccct gcctttctct gcggccgcat
     2521 ccccggcctt tgccccgggc ttgagcgctg cgggaggcgc acaaaaagct ttcaaaagaa
     2581 aaacacgttc tgaaaccgat gccgcacatc gtttccggaa cgtgtttttt gatgctttta
     2641 aggatttgtt tcacccggca cccctccgga ccccgccggg gcggcgccga gatgggtcag
     2701 ggacggatat aggcatatcc cgcctgctgc ttttgggcca gctccaccac gcctgcgggc
     2761 accgtcaccg cgaactgcgc cagcgctttc ggctccagcc gctgcgcccg cagcgcgttg
     2821 cggcaggccg cgaacacaac gcctttttcc gccagggcgg cgatccgctg cagcagcgcc
     2881 ccgtctccct gccccggcgc cag
//
LOCUS       DBMDYA010000361         2898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7607_length_2898_cov_1.474079, whole genome shotgun sequence.
ACCESSION   DBMDYA010000361 DBMDYA010000000
VERSION     DBMDYA010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_2898_cov_1.474079"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="ACZPFK_09080"
     CDS             <1..192
                     /locus_tag="ACZPFK_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406506.1"
                     /translation="LNGRAVFVDDRALKARALEEDPGLVPIYHAPDYPGFALFYIDLR
                     EVKTYTFADDVTVYKVNGG"
     gene            692..2059
                     /locus_tag="ACZPFK_09085"
     CDS             692..2059
                     /locus_tag="ACZPFK_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406507.1"
                     /translation="MKRSPAADMTTGNVTRHILRFALPLLVGNIFQQFYNAADSIIVG
                     RFVGKQALAALGVAAPVMNIVLFTVIGLCTGASIFMATLWGEGDLDKCRREFSTCVLA
                     GLAFTAVVSLAAILCIRPILWAMRTPAELIADATGYLRVIFGGLVCSFLYNVYTAALR
                     AAGDSASPLYFLVLSSAINVLLDLLFVPVLGWGILGAAAATVLAQGLSAALCYLYTAR
                     RVPQLHLRRCDLVLDRALLRATVGYSQSAALQQTCFFVGKLLLQGAVNPLGTDAIAAF
                     NAVSRIEGFVLAPGNSIATAVTTFIAQNRGAKRPERIFAGFRQGCRMAWGYGVLMAAS
                     MYLLAAPFTALFVNAGETAVVSEGVRYLHAMSVFYLMPAMGDMMQGFFRGIGKLNVSL
                     FGTIFQISVRVGLTYLLAGSLGIVSVCIATGTGWTLFLLALALLYRREKPRVWKTLSA
                     AAPAV"
     gene            complement(2471..2896)
                     /locus_tag="ACZPFK_09090"
     CDS             complement(2471..2896)
                     /locus_tag="ACZPFK_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR1406508.1"
                     /translation="MDADGCPVVRQALAIAAQRGVQAVVVCDTAHRIDCPGAELVMVD
                     QGPDSADLALANRLRPGDVAVTQDFGLAALCLARGARALDQNGRRYTPDNIGPLLEQR
                     AFAGKLRRAGRHGKGPAKRTPAQDAAFCAALEALLEQPG"
BASE COUNT          424 a         1020 c          893 g          561 t
ORIGIN      
        1 ctgaacggcc gcgccgtctt tgtggatgac cgcgccctca aggcccgcgc cctggaggag
       61 gaccccggcc tggtgcccat ctaccacgcg ccggactacc ccggcttcgc cctgttctac
      121 atcgacctgc gggaggtcaa gacctatacc tttgccgacg acgtgaccgt gtacaaggtg
      181 aacgggggct gagaggcctt tcacgtcctg ctgtgcggga ggagcttttt taaatcctca
      241 aaaaaagaaa aaaactgcgc tggaaagcta aaaagcgctg aactgcaaat taaaaaagag
      301 cggaggcgat acgaaatgta tcatccccgc tctttttgct cgggcgctcc acacctcttt
      361 ttcatcctct ccgggcttca cctcactttc ctgccctccg cgctcctacc tgccgggccg
      421 caggccgccg gggagcggcc tgggttgaca acggggggcg cgggggcgca aaataagagg
      481 tacaaggctg ctttttccct cttcgctttt tccctgctct gccgggcccc gtcaagggcg
      541 cgaggagagg ccttgcgcgg cggcgtccgg cctgggaccg gctctcaaaa ttcaaatcaa
      601 atcgcctcac ccggccccgc cgccctttta tttctctttt acagcaatca ttccgcgcct
      661 ttgatcctgg cgtgaaagga gcgagcccgt tttgaaacga tcccccgccg ccgacatgac
      721 caccggcaac gtcacccgcc acatcctgcg ctttgcgctg ccgctgctgg tgggcaatat
      781 ttttcagcag ttctacaacg ccgccgactc gatcatcgtg ggccggtttg tgggcaagca
      841 ggctctggcc gccttggggg tggccgcgcc ggtgatgaac atcgtgctgt tcaccgtcat
      901 cggcctgtgc accggggcct ccatctttat ggcgaccctc tggggcgagg gcgacctgga
      961 caagtgcaga cgcgaatttt ccacctgcgt gctggccggt ctcgctttta cggccgttgt
     1021 ctcgctggcg gccatcctct gcatccgccc catcctctgg gccatgcgca cccccgccga
     1081 gctgatcgcc gacgcgacgg gctatctgcg ggtgatcttc ggcggtttgg tatgcagctt
     1141 tctctacaac gtttacaccg ccgcgctgcg ggccgccggc gactccgcct ccccgctcta
     1201 tttcctggtg ctctcctccg ccatcaacgt gctgctggac ctgctgttcg tgcccgtgct
     1261 gggctggggc attctcgggg cggcggcagc caccgtgctg gcccagggcc tgtcggcggc
     1321 gctctgctac ctttataccg cgcggcgggt gccgcagctg cacctgcgcc gctgtgatct
     1381 ggttctggac cgcgccctgc tgcgcgccac cgtgggctac agccaatccg ccgccctgca
     1441 gcagacctgc tttttcgtgg gcaagctgct gctgcagggc gctgtcaacc cgctgggcac
     1501 cgacgccatc gccgcgttca acgccgtcag ccgcatcgag ggcttcgtgc tggccccagg
     1561 caacagcatc gccacggcgg tgactacctt tatcgcccag aacaggggtg caaaaaggcc
     1621 ggagcgcatc tttgccggtt ttcggcaggg ctgccgcatg gcctggggct acggggtatt
     1681 gatggcggcg tcgatgtatc tgctggccgc ccctttcacc gccctgttcg tcaacgccgg
     1741 tgagaccgcc gtggtgagcg agggggtgcg ctatctgcac gccatgagcg tcttttacct
     1801 catgcccgcc atgggggata tgatgcaggg ctttttccgc ggcatcggca agctgaacgt
     1861 ctccctcttc ggcaccatct ttcagatctc ggtgcgcgtg gggctgacct atcttttggc
     1921 gggcagcctg ggcatcgtca gcgtctgcat cgccaccggc acaggctgga ccctttttct
     1981 gctggcgctg gcgctgctct accggcggga gaagccccgc gtctggaaaa cgctttcagc
     2041 cgccgcccct gcggtataag ggtgaagacg ccatttcttg acactgcacc agggcaggca
     2101 tcagcttcat ccgctccttg tcggggcccc gcggcggagg cgcggaggcg gccgcgcagg
     2161 ccccggcaga tgcctccgct ccatggcggt cgcccccggc ctccccatgc gcggctgacc
     2221 cccccttgtg cggccgattc tcccgtgcgg ttgccccgcc gtgtatggct gccctgcctg
     2281 tgcgacaccc cgctgtgtgc ggctgccctg gccgcatggc ctcccctggg ccccacggcg
     2341 ggacttgacg aaaaaagcgt ttaacaccca gccgcaggca cgctcaaaac aaaatttccg
     2401 tctaaaaaac gccctttccc atccgggagg ggcgtttggg ccggtgcgcc tgtgggtgct
     2461 gcccaaaatc tcagcccggc tgctccagca gcgcctccag cgccgcgcag aacgccgcgt
     2521 cctgcgccgg ggtgcgcttg gcaggcccct tgccgtgccg gcccgcacgc ctcagcttgc
     2581 cggcaaaggc ccgctgctcc agcagcgggc cgatgttgtc aggggtgtac cggcgcccgt
     2641 tctgatccag cgcgcgggcg ccccgcgcca ggcagagggc agccaggcca aaatcctggg
     2701 tcaccgccac gtcgccgggg cgcagccggt ttgccagggc caggtccgcg ctgtccggcc
     2761 cctggtccac catcaccagc tccgcccccg ggcaatcgat gcggtgggcg gtgtcgcaga
     2821 ccaccacggc ctgtaccccg cgctgggcgg cgatggccag ggcctgccgc accacggggc
     2881 agccgtccgc gtccagat
//
LOCUS       DBMDYA010000362         2895 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7624_length_2895_cov_1.372584, whole genome shotgun sequence.
ACCESSION   DBMDYA010000362 DBMDYA010000000
VERSION     DBMDYA010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_2895_cov_1.372584"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="ACZPFK_09095"
     CDS             complement(<1..183)
                     /locus_tag="ACZPFK_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406509.1"
                     /translation="MDTKSTKNRWVAVLLCLLMMAAGAAGLVGLAPVYYEQRWSGNEA
                     LFNGDYFLRLLATSNYT"
     gene            complement(158..865)
                     /locus_tag="ACZPFK_09100"
     CDS             complement(158..865)
                     /locus_tag="ACZPFK_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1406510.1"
                     /translation="MSYRILVVEDDREIRDGIGIYLKNQGYEVVKAANGAEGLAALRE
                     NEIHLAIVDIMMPVMDGVTMTMKLRESHDFPVIMLSAKSEDIDKITGLNIGADDYVTK
                     PFAPMELLARVNSQLRRYAKYLALAGGQNDRPEEHVYTIGGLELNEDTVSLAVDGRPV
                     RLTPLEFKILALLMKNPGRVFSAEEIYERVWNEKAIATDTIMVHIRNIREKIEINPRE
                     PKYLKVVWGIGYKIDQE"
     gene            complement(1099..1638)
                     /locus_tag="ACZPFK_09105"
     CDS             complement(1099..1638)
                     /locus_tag="ACZPFK_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1406511.1"
                     /translation="MRMEETDMTQERFEEMVKQYGRLVFTVCNQLVGDAGQAEDLAQE
                     TFLSAWLHADRCPEGAEKAWLCRIAANKAKDCLKSAYRRRVTAAEEPGDWLQGQAGCA
                     AAVSPEDAFASRETLHAVRGGIEGLRAPYKEVSALFFLENYSVAEIARLLGRPHKTVN
                     TQLYRARSMLRGQLAAQVA"
     gene            1787..2236
                     /locus_tag="ACZPFK_09110"
     CDS             1787..2236
                     /locus_tag="ACZPFK_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HIR1406512.1"
                     /translation="MSDWSKLYPPERRPSAEELRTQAATPLWQELCGYLESAYAVTPR
                     TEYSRCGSLPGWNVKYKKAGKALCTLYPGWPAPGSFTCMVVAREKDEQEVALALAGCT
                     PAVQQLFETTAYFNGGKWLMIRVDSPDALRDVKVLLAVRARPAAATL"
     gene            2300..2857
                     /locus_tag="ACZPFK_09115"
     CDS             2300..2857
                     /locus_tag="ACZPFK_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIR1406513.1"
                     /translation="MELLDEIREQARQAVSELLAEARLEPGDLFVVGCSSSEIMGRRI
                     GQGSSLEAAAAVCDGILPVLEESGLFLAAQCCEHLNRALIVERAALPRFAEVVNVAPQ
                     PHAGGSFATTVYGRMREPVAVEQVRAGAGLDIGGTLIGMHLRPVAVPVRLSLSRIGEA
                     GLLCARTRPKYIGGSRAAYLPGSGR"
BASE COUNT          526 a          941 c          871 g          557 t
ORIGIN      
        1 ggtgtagttg ctggtggcca gcagccgcag aaagtagtcc ccattgaaaa gcgcctcgtt
       61 gccggaccag cgctgttcgt aataaacggg cgccagcccc accagccccg cggcccccgc
      121 ggccatcatc agcaggcaga gcagcacggc cacccatcta ttcttggtcg attttgtatc
      181 caatccccca taccaccttt aaatattttg gctcccgggg gttgatctcg atcttctccc
      241 ggatattgcg gatatgcacc atgatggtgt cggtcgcgat cgccttttcg ttccagaccc
      301 gctcatagat ctcctctgcg gagaacaccc ggcccgggtt tttcatcagc agggccaata
      361 tcttgaactc cagcggggtg agccgcaccg ggcggccgtc cacggccagc gagacggtgt
      421 cctcgttcag ctccaggccg ccgatggtgt agacgtgctc ctccggccgg tcgttctgcc
      481 cgcccgcaag ggccagatat tttgcatacc gccgcagctg cgagttcacc cgcgcgagca
      541 gctccatggg tgcaaagggc ttggtcacat agtcgtccgc cccaatattg aggccggtga
      601 tcttgtcgat gtcctctgat tttgccgaga gcatgatgac ggggaagtcg tggctctcgc
      661 gcagcttcat ggtcatggtg acgccgtcca tcaccggcat catgatatcc acaatggcga
      721 gatggatctc gttttcccgc agggcggcca gtccctctgc cccattggcc gccttgacga
      781 cctcatagcc ctggtttttc aggtaaatgc cgatgccgtc ccggatttcc ctgtcgtcct
      841 cgacgaccag aatgcgatag ctcatagttt cctcccggct ggttttttaa acttgtctcc
      901 attactgatc tattatacat ggaaacaccg cgaaaaacat ccccgggaca aagacgagcc
      961 tgcataagaa aataagagct cagccatatg attttgacgc agtgcgggtt cccacccgcg
     1021 ggacaagcga aagctgagcc ctaaataaga aagggctccc ctgacgccag gatggaaaca
     1081 gggcgcggcc gttttccctc acgccacctg tgcggcgagc tggccgcgca gcatgcttct
     1141 tgcgcggtag agctgcgtgt tcacggtctt gtggggccgc cccagcagcc gggcgatctc
     1201 ggcgacggaa tagttttcga gaaaaaacag ggccgagacc tccttgtagg gcgcgcgcag
     1261 cccctcaatg ccgccgcgca ccgcgtgcag cgtctcgcgg gaggcaaagg catcctcggg
     1321 cgacacagcg gcggcacagc ccgcctgccc ctgcagccag tcgcccggct cctcggcggc
     1381 ggtgaccctg cggcgatagg cgcttttgag gcagtctttg gccttgttgg cggcgatgcg
     1441 gcacagccac gccttttccg ccccctccgg gcagcggtcc gcgtgcagcc atgcggagag
     1501 gaacgtctcc tgtgccagat cctccgcctg gccggcgtcc cccaccagct ggttgcacac
     1561 ggtaaacacc agccgcccgt actgcttcac catctcttca aaccgctcct gcgtcatgtc
     1621 cgtctcctcc atccgcatgt tttctcactc attgccccta tcttaccgga caaatctaaa
     1681 aagcccggac ataaatttcc aaagaatttc taaagaatcc ctttggattt tcggtagctg
     1741 ttgttataat ggagaaaaca gcgggccccg cccggaaagg acttttatga gcgattggag
     1801 caagctttac cccccggagc gccggccctc tgccgaggag ctccgcaccc aggcggccac
     1861 gccgctttgg caggagctgt gcgggtatct cgagagcgcc tatgccgtca ccccccgcac
     1921 cgaatacagc cgctgcggca gcctgccggg ctggaacgtc aaatacaaaa aggccggcaa
     1981 agccctgtgc accctctatc ccggctggcc cgcgcccggc agcttcacct gtatggtggt
     2041 cgcccgcgaa aaagacgagc aagaggtcgc cctcgccctt gcaggctgca cgccggccgt
     2101 gcaacagctg ttcgagacca ccgcctattt caacggcggc aaatggctga tgattcgggt
     2161 ggattccccc gatgccctgc gggacgtcaa ggtcctgctc gcagtgcggg cccggccggc
     2221 ggccgcgacg ctgtgagacg ggccgatgtc ggctgcggca gagaaagtct ttatttccta
     2281 tattgcggag gcgaacgaaa tggaattgtt ggatgagatc cgagaacagg cgcggcaggc
     2341 tgtgagcgag ctgctggccg aggcgcgcct tgagccgggc gacctgtttg tggtgggctg
     2401 ttcctccagc gagatcatgg ggcggcgcat cggccagggc tccagtctgg aggcggccgc
     2461 tgccgtctgc gacggcattc tgccggtgct ggaagaaagc ggcctgtttc tggccgccca
     2521 gtgctgcgag cacctcaacc gcgccctcat tgtggagcgc gccgcgctgc cccgctttgc
     2581 ggaggtggtc aacgtggcgc cccagcccca cgcgggcggc tctttcgcca ccaccgtcta
     2641 cggccgcatg cgtgagccgg tggcggtgga gcaggtgcgg gccggggcgg ggctggacat
     2701 cggcggcacc ctgatcggca tgcatctgcg gccggtggcg gtaccggtgc ggctgagcct
     2761 ttcccgcatc ggcgaggcgg gcctgctctg cgcccgcacc cggcccaaat acatcggcgg
     2821 cagccgcgca gcctatctgc ccggctccgg cagataaggg ggcaaaaatg gaaaacgaaa
     2881 agcaaaagct gctgc
//
LOCUS       DBMDYA010000363         2886 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7646_length_2886_cov_1.180610, whole genome shotgun sequence.
ACCESSION   DBMDYA010000363 DBMDYA010000000
VERSION     DBMDYA010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_2886_cov_1.180610"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="ACZPFK_09120"
     CDS             complement(<1..300)
                     /locus_tag="ACZPFK_09120"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR1406514.1"
                     /translation="MAKLFFRYGAMNSGKSTALMQAAHNYEERGMRVLVLKPKVDTRG
                     GGRIVSRLGVSRTADLLITPEMDLLAEIRRDIARSGPLACVLVDESQFLRRAQVDQ"
     gene            complement(396..1031)
                     /locus_tag="ACZPFK_09125"
     CDS             complement(396..1031)
                     /locus_tag="ACZPFK_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1406515.1"
                     /translation="MPTQNKPSLPVIKRLPKYYRYLGEMEKEGVTKVSSSELARVMGT
                     TASQVRQDFNCFGGFGQQGIGYNVKLLHEELGKLIFARHPVPTILIGAGNLGHAMANY
                     FRQDTQGFELLAAFDVKPEMIGLTLCGVAIRDMAELEAYCAEHKPKVAVICVPREAAK
                     QVAQKLIDLGIRAFWNFSHYDFSIEYTDIIVENVHLGDSLATLSYEVNNNL"
     gene            complement(1078..2235)
                     /locus_tag="ACZPFK_09130"
     CDS             complement(1078..2235)
                     /locus_tag="ACZPFK_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR1406516.1"
                     /translation="MEINTAVEHFKRVLESQEQRAAEMRAQGGFVDYAKLPKIVVGIC
                     GGDGIGPVITHEAERVLRFLLADEVAAGRVEFKEISGLTIENRAACGKAIPDDVMQEL
                     KACHVILKGPTTTPRAGDGWPNIESANVAMRKALDLFANVRPVKIPEEGIDWTFFREN
                     TEGGYALGSQGLELDDGVAFDFTVTTTEGTQRIAELAYDYAKKNGKNRVSIITKANVI
                     KTTDGKFLKLCQEVGKRYPEIATDDWYIDILTAKLIDKQRRKDFQVLVLPNLYGDIVT
                     DEAAEMQGGVGTAGSANIGKKYAMFEAIHGSAPRMVAEGRAKYADPCSMLRATVLLLS
                     HIGRQKQADALEKALDTCMFTEKRLTITGRDTGCTCEEFGDYVMETVKAAE"
     gene            complement(2239..>2886)
                     /locus_tag="ACZPFK_09135"
     CDS             complement(2239..>2886)
                     /locus_tag="ACZPFK_09135"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325414.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1406517.1"
                     /translation="HDNMISMPASEAEYEQVEILRGPNIQPLPKAMVPEDHMALPVLL
                     KVGDNISTDHIMPAGAKVLPFRSNIPKIANFCFEKCDPTFHDRALAADGGVIVGGSNY
                     GQGSSREHAALVPLYLGIKAVVAVSFARIHKANLVNAGILPLEFADAADYDKVQQGDE
                     IELDGVLAGIREGRMTLKNVTRGECYPLVCALSERQCAMIAAGGLLNYTKMKSEG"
BASE COUNT          482 a          909 c          895 g          600 t
ORIGIN      
        1 ctgatccacc tgggcgcggc gcaaaaactg gctctcgtcc accagtacgc aggcaagggg
       61 cccgctgcgg gcgatatccc gccggatttc agcaagcagg tccatttccg gagtgatgag
      121 caggtcggct gtgcggctga cccccagacg gctcacgatg cggccgccgc cccgggtgtc
      181 caccttgggc ttgagcacca gtacccgcat gccgcgctct tcataattgt gggccgcctg
      241 catcagggcg gtggatttgc cgctgttcat cgccccgtat cgaaaaaaca attttgccat
      301 ctgcaaagac catccttttg ccgctttgcc gcggccagag taaaagaaaa ggcggactcg
      361 gcgaaagcca aggccgccgc ggcgcaaaag cgccgttaca gattgttgtt gacctcatag
      421 ctcagcgtgg ccagactgtc gcccagatgc acgttttcca cgatgatgtc ggtgtattcg
      481 atggaaaaat catagtggct gaagttccaa aaagcgcgga tccccaggtc gatcagcttt
      541 tgcgccacct gctttgcagc ctcacggggc acgcagatga ccgccacctt gggcttgtgc
      601 tcggcgcaat aggcctccag ctccgccatg tcccgaatcg ccaccccgca cagggtcagg
      661 ccgatcatct ccggcttcac gtcaaaggcg gccagcagct caaagccctg ggtgtcctgg
      721 cgaaaatagt tcgccatcgc atggcccagg ttgcccgcgc cgatgaggat ggtgggcacg
      781 ggatggcgcg caaagatcag cttgccgagc tcttcatgca gcagcttgac attgtaaccg
      841 atgccctgct ggccgaagcc gccaaagcag ttgaaatcct gccgcacctg cgaggccgtc
      901 gtacccatca cccgggccag ttcagaggag gagaccttgg tcacgccctc tttttccatc
      961 tcgcccagat agcggtaata cttcggcagc cgtttgatga ccggcaggga gggcttgttc
     1021 tgagtgggca ttgctcttgt caccttgtat tcttagattt tgcgaaagag gcggggatta
     1081 ttccgccgct ttcacggtct ccatcacata atcgccaaac tcctcacagg tgcagccggt
     1141 gtcgcggccg gtgatggtca ggcgcttttc ggtgaacatg caggtgtcca gcgccttttc
     1201 cagcgcgtcc gcctgctttt ggcggccgat atgggatagc agcagcaccg tggcccgcag
     1261 catgctgcag gggtccgcgt actttgcccg accctcggcc accatgcgcg gcgccgagcc
     1321 gtggatggcc tcgaacatgg cgtacttttt gccgatgttg gcgctgcccg cggtgccgac
     1381 gccgccctgc atctcggcgg cctcgtcggt gacgatgtcc ccgtacaggt tcggcaggac
     1441 cagcacctga aaatccttgc ggcgctgctt gtcgatcagc tttgcggtca ggatgtcgat
     1501 gtaccagtcg tcggtggcga tctccgggta gcgcttgccc acctcctggc agagcttcaa
     1561 aaatttgccg tccgtggtct tgatcacatt ggccttggtg atgatggaga cccggttttt
     1621 tccgttcttt ttggcgtagt cgtaggccag ctcggcaatg cgctgggtgc cctcggtggt
     1681 agtgacggta aagtcgaacg ccacgccgtc gtccagctcc agcccctggc tgcccagggc
     1741 atagccgccc tcggtgttct cgcggaaaaa ggtccagtcg atgccctcct cggggatctt
     1801 caccggccgc acgttggcaa acaggtccag cgccttgcgc atggccacgt tggcgctttc
     1861 gatgttgggc cagccgtcgc cggcgcgcgg ggtggtggtg gggcctttca aaatgacatg
     1921 gcaggctttc agctcctgca tcacgtcgtc ggggatggcc ttgccgcagg cggcgcggtt
     1981 ttcgatggtc aggccgctga tctccttgaa ctccacccgg cccgccgcca cctcgtccgc
     2041 cagcaaaaag cgcagcaccc gctcggcctc gtgggtgatc accgggccga tgccgtcgcc
     2101 gccgcagatg ccgacgacga tcttgggcag cttggcgtag tccacaaaac cgccctgggc
     2161 gcgcatctcg gccgcgcgct gctcctggct ctccaatacc cttttaaagt gctcgacagc
     2221 ggtgttgatc tccatttttt acccctcgct tttcattttg gtgtagttga gcaggccgcc
     2281 ggcggcgatc atggcgcact ggcgctccga cagtgcgcag accagcggat agcactcgcc
     2341 gcgggtcacg tttttcaggg tcatgcgccc ctcgcggatg ccggcgagca cgccgtccag
     2401 ctcgatctcg tccccctgct gcaccttgtc atagtcggcg gcgtcggcaa actccagcgg
     2461 caggatgccg gcgttgacta ggttggcctt gtggatgcgg gcaaagctga ccgcgaccac
     2521 ggccttgatg cccagataca gcggcaccag ggcggcgtgc tcccggctgg agccctggcc
     2581 gtagttggag ccgcccacga tgacgccgcc gtcggcggcc agagcccggt cgtggaaggt
     2641 cgggtcgcat ttttcaaagc agaagttggc gatcttgggg atattgctgc ggaaaggcag
     2701 caccttggcg cccgcgggca tgatgtggtc ggtggagatg ttgtcgccca ctttcagcag
     2761 caccggcagc gccatgtgat cctcgggcac catggccttg ggcaggggct ggatgttggg
     2821 cccgcgcagg atctcgacct gctcgtattc ggcctcggag gcgggcatcg agatcatgtt
     2881 gtcgtg
//
LOCUS       DBMDYA010000364         2883 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7656_length_2883_cov_1.750539, whole genome shotgun sequence.
ACCESSION   DBMDYA010000364 DBMDYA010000000
VERSION     DBMDYA010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7656_length_2883_cov_1.750539"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            200..1420
                     /locus_tag="ACZPFK_09140"
     CDS             200..1420
                     /locus_tag="ACZPFK_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1406518.1"
                     /translation="MTKIDIFSGFLGAGKTTLIKKLIKEAFGGEKLVLIENEFGEIGI
                     DGGFLKEAGIQITEMNSGCICCSLVGDFGKALEKVLDQYAPDRILIEPSGVGKLSDVI
                     RAVQRVGEHADIRLNSFTTVADANKCRVYMKNFGEFYDDQVSHANTIILSRTQGMDEA
                     KLEAAVALLKAKNATATVITTPWDELAGEQILSAMERRSTLEEELKLLVAEAEHDHDH
                     AHEHEEHGHHHHHDGECDDPECSCHDHDHEHDHEEHERHHHHDGECDDPECSCHDHEH
                     EHDHEEHEHHHHHDGECDDPECSCHDHEHGHDHHHHHADEVFTSWGAETPRKYTDAEV
                     QFALDALTDAVSYGVVLRAKGILPATDGGWIHFDYVPGESNVRRGGADVTGRLCVIGS
                     KLDEENLAELFRLA"
     gene            1435..2373
                     /locus_tag="ACZPFK_09145"
     CDS             1435..2373
                     /locus_tag="ACZPFK_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR1406519.1"
                     /translation="MIPLYLFTGFLDSGKTSFIQETLADPRFSSGEKTLLLLCEEGET
                     EYDPDKFAGGNVTLVTISSPEEMTAEYLRNLTAKHHSDRVLLEYNGMWPMSVLVTHMP
                     KNWQLYQHIMLADASTFEMYNANMRSLVVERLSSAEMVLFNRCGADTDKMALHRIVRG
                     SNRRSEIAYEYENGSVDYDEIEDPLPFDLQADPVVIEDDDFGLWYMDITDDPQKYAGK
                     RVRFLAQVAQSPRVPKGAFASGRFVMTCCVEDITFVGLVCKYEKAASLKSRDWVTVTA
                     RVKIEYAAIYGGKGPVLVAETVEPAAKPEQEVVYFS"
BASE COUNT          559 a          910 c          948 g          466 t
ORIGIN      
        1 ccacaatgct tatcttttgg tctttggttc ggaatagtgt agtgctggcg gtctatctct
       61 tgttttcggt tgcttgcttc cttaccgtac atgagcattg acggcgggta tataataaaa
      121 aggcggagca tgtgagagcc cggcgggctg ccggggccgc ggcggccgca gtatttgaca
      181 gaaagacggg aagttgcaaa tgacaaaaat tgatattttc tccggtttcc tcggggccgg
      241 caagacgacc ctcatcaaaa agctcatcaa agaggcgttc ggcggcgaaa agctggtgct
      301 catcgagaac gaattcggcg agatcggcat cgacggcggc tttctcaagg aggcgggcat
      361 ccagatcacc gagatgaact ccggctgcat ctgctgcagc ctggtgggcg atttcggcaa
      421 ggcgctggaa aaggtgctgg accagtatgc ccccgaccgc atcctcatcg agccctccgg
      481 cgtgggcaag ctgtccgacg tcatccgcgc cgtgcagcgg gtgggcgagc acgccgacat
      541 ccggctgaac agcttcacca ccgtggccga cgcgaacaag tgccgcgtct atatgaagaa
      601 cttcggcgag ttctacgacg accaggtcag ccacgccaac accatcatcc tcagccgcac
      661 ccagggcatg gacgaggcca agctggaggc cgcggtggcc ctgctgaaag cgaaaaacgc
      721 cactgccacc gtcatcacca ccccctggga tgagctggcc ggcgaacaga tcctctcggc
      781 catggagcgc cgctccaccc tggaggagga gctgaagctg ctggtggccg aggcggagca
      841 cgaccacgac catgcgcacg agcatgagga acacgggcac caccatcacc acgacggcga
      901 gtgcgacgac ccggagtgca gctgccacga ccacgaccat gagcacgacc acgaggaaca
      961 tgagcgccac catcaccacg acggcgagtg cgacgacccg gagtgcagct gccacgacca
     1021 cgagcatgag cacgaccacg aggaacatga gcaccaccat caccacgacg gcgagtgcga
     1081 cgacccggag tgcagctgcc acgaccatga gcatgggcac gaccaccatc accaccacgc
     1141 ggacgaggtg ttcaccagct ggggcgccga gaccccgcgc aagtacaccg acgcggaggt
     1201 gcagttcgcg ctggacgccc tcaccgacgc ggtgagctac ggcgtggtgc tgcgggccaa
     1261 gggcatcctg cccgccaccg acggcggctg gatccacttc gactatgtgc cgggcgagag
     1321 caatgtgcgc cgcggcggcg cggacgtcac cggccgcctg tgcgtcatcg gctcgaagct
     1381 ggacgaggag aacctcgccg agctgttccg gctggcctga acgggggtgg cgagatgatc
     1441 ccgctttatc tgttcaccgg ctttctggac agcggcaaga ccagctttat ccaggagacg
     1501 ctggccgacc cgcgcttttc cagcggcgaa aagacgctgc tgctgctctg cgaggagggc
     1561 gagaccgagt atgaccccga caaattcgcg ggcggcaacg tcacccttgt gaccatctcc
     1621 tcgcccgagg agatgaccgc cgagtacctg cgcaacctga ccgccaagca ccacagcgac
     1681 cgggtgctgc tggagtacaa cggcatgtgg cccatgtcgg tgctggtgac ccatatgccg
     1741 aaaaactggc agctctacca gcacatcatg ctggccgacg cctccacctt tgagatgtac
     1801 aacgccaaca tgcgcagcct ggtggtggag cggctgtcca gcgccgagat ggtgctgttc
     1861 aaccgctgcg gcgcggacac cgacaagatg gccttgcacc gcatcgtgcg cggctcgaac
     1921 cggcgcagcg agatcgccta tgagtatgag aacggcagtg tggactacga cgagatcgag
     1981 gacccgctgc cctttgacct acaggccgac ccggtggtga tcgaggacga cgactttggc
     2041 ctgtggtata tggacatcac cgacgacccg cagaaatatg cgggcaagcg ggtgcgcttt
     2101 ttggcccagg tggcccagtc cccgcgggtg ccgaaggggg cctttgcgtc cggccgcttt
     2161 gtgatgacct gctgtgtgga ggacatcacc tttgtgggcc tggtctgcaa atacgaaaag
     2221 gcggcgagcc tgaaaagccg cgattgggtg accgtgaccg cgcgggtgaa gatcgagtac
     2281 gccgccatct acggcggcaa gggcccggtg ctggtggccg agacggtgga gcctgccgca
     2341 aagccggagc aggaggtcgt ctatttcagc tgaaaagcgc cgccccgctt cggcccggtt
     2401 tgagaccggc tgaaacgggg cggcgctttt ggcgcggacc gggtgcggcg gccgcctgat
     2461 tttggcgaaa aaggcccctc tgccgcgccg gcgctgcccc gggccgccaa ggcttgcggc
     2521 gcgcagcttg ccggcgggcg agcctttttc acggacggcg gatgcggtac ggctttgcct
     2581 tgcgaggcaa aacccgcccc cctcaggctc tcgctccccg gggcttttgc gtcgcccgct
     2641 ccgggagagg cagcccgcag cgggcccgcg gtcgggaggg tggctgaggg ccgcctcggc
     2701 gccgcaggct tcgcccggcg gcggaagcgg cagggcggag cccggtgcga ggcaaacaag
     2761 caaagaggcg ggccggtatc caaccggccc gcctctttgc ttgtcctttt gacggaatgg
     2821 gttcaccgct cctcgatggt gtaggtgaac ttgtactcct gctgcagggc cttcaccttg
     2881 tcc
//
LOCUS       DBMDYA010000365         2880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7661_length_2880_cov_1.483627, whole genome shotgun sequence.
ACCESSION   DBMDYA010000365 DBMDYA010000000
VERSION     DBMDYA010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7661_length_2880_cov_1.483627"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="ACZPFK_09150"
     CDS             complement(<1..864)
                     /locus_tag="ACZPFK_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406520.1"
                     /translation="MKSALSRHLYTDPAFYKSLVAITLPIALQNVISLGVNMMDTVML
                     GQLGDVAIAAANLGGQPFMILNVLGFGLASGASVLIAQYWGRRDMARIRQLFTITLRG
                     ALAASALFCLGCLCFPQGILSIYTPEPEVIRAGADYLRILALSFVLYSFSNCYIMCLR
                     AVEQVKVSMVVYGLSFFVNVFFNWCFIFGNLGFPALGVRGAAVGTLIARGTECVLVVL
                     YMLFVEKKVRFTPATLFGSTGGLLGDFVKNSVPVIGNELLWGVGFSVLNMIMGRMGAG
                     FVTAYGIANTVN"
     gene            complement(937..1497)
                     /locus_tag="ACZPFK_09155"
     CDS             complement(937..1497)
                     /locus_tag="ACZPFK_09155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1406521.1"
                     /translation="MLILASGSPRRRELLQLVTPRFEVVASEVDERAVSAPTPQALVA
                     ALAEAKARAVAALRPADVVLGCDTVVDLDGRVLGKPADRAEAEAMLRAYSGRWHYVRT
                     GVCAVKDGQAHGFVETSKVFFAPLEEDEIAAYAATDEPYDKAGAYAIQGGAARFVTRV
                     EGCYFNVMGLPVAALYRLLRQLQALG"
     gene            complement(1717..1977)
                     /locus_tag="ACZPFK_09160"
     CDS             complement(1717..1977)
                     /locus_tag="ACZPFK_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR1406522.1"
                     /translation="MKRNFFFLFYLLAGIIVGSLLSNLCAGVPFLSWLAYSGTIGFAA
                     GDPACLDLIVFKLYFGFSIGVSVAQIICIALAMFLYNRTRVR"
     gene            complement(1991..2440)
                     /gene="dut"
                     /locus_tag="ACZPFK_09165"
     CDS             complement(1991..2440)
                     /gene="dut"
                     /locus_tag="ACZPFK_09165"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1406523.1"
                     /translation="MDCNVKVKLLDPAAKLPQRATPGSAAADLCALCPPQGVLLPAHG
                     RAVLPTGIAIELPGPGYVALVFARSGLGIKKGITLSNSVGVIDSDYRGELRVGLVNLS
                     DEDYTVQNGERIAQLAVLPVAQAAFAAAEELGETQRGEGGFGSTGRL"
     gene            complement(2647..>2880)
                     /locus_tag="ACZPFK_09170"
     CDS             complement(2647..>2880)
                     /locus_tag="ACZPFK_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1406524.1"
                     /translation="DRKGRRLTVACAVPARELLNPVPLWLGDRRREVGTDFTVLYFTG
                     ESAARAAEVARLYRQGLPFDGEFTRGLYYKTVE"
BASE COUNT          499 a         1003 c          935 g          443 t
ORIGIN      
        1 gttcacggtg ttggcgatgc cgtaggccgt cacaaagccc gcgcccatgc ggcccataat
       61 catgttcagc accgaaaagc ccacgcccca cagcagctcg ttgccgatca ccggcacgct
      121 gtttttgaca aaatcgccca gcagcccgcc ggtggagccg aacagggtgg ctggggtgaa
      181 gcgcaccttt ttttccacaa agagcatgta gagcaccacc agcacgcact cggtgccgcg
      241 ggcgatcagg gtgcccaccg ccgcgccccg cacccccagc gccggaaagc ccaggtttcc
      301 gaagatgaag caccagttga aaaagacgtt gacgaaaaag ctgagcccgt acaccaccat
      361 gctcaccttg acctgctcca ccgcccgcag gcacatgatg tagcaattgg aaaaggagta
      421 gagcacaaag ctcagcgcca ggatgcgcag atagtccgcc ccggcgcgga tgacctccgg
      481 ctcgggggta tagatggaca aaatgccctg ggggaagcac aggcagccca ggcagaacag
      541 ggccgaggcg gccagcgcgc cccgcagcgt gatggtgaag agctggcgga tgcgggccat
      601 gtcccggcgg ccccagtact gcgcgatgag caccgaggcg ccgctggcca ggccgaagcc
      661 cagcacgttg aggatcataa agggctggcc gcccaggttt gccgcggcga tggccacgtc
      721 ccccagctgg cccagcatca ccgtgtccat catgttgacg cccaggctga tgacgttctg
      781 cagcgcaatg ggcagggtga tggcgaccag acttttataa aaggccgggt cggtgtaaag
      841 atgacgggaa agggcggatt tcatatggcg tcttgattct ctctttctct tgattctcgt
      901 cttgccccgc ttttcagcgc ccggggcgcc gcggcctcag cccagcgcct gcagctgccg
      961 cagcaggcgg tagagggcgg ccaccggcag gcccatgaca ttgaagtagc agccctcgac
     1021 gcgggtgacg aaccgcgcgg ccccgccctg gatggcgtag gcccccgcct tgtcgtaggg
     1081 ctcgtcggtg gccgcatagg cggcgatctc gtcctcctcc agcggggcga aaaagacctt
     1141 gctggtctcc acaaagccgt gggcctgccc gtctttcacg gcgcagacgc cggtgcgcac
     1201 atagtgccag cggcccgagt aggcgcgcag catggcctcg gcctcggccc ggtccgccgg
     1261 cttgcccagc acccggccgt ccagatccac cacggtgtcg cagcccagca ccacgtccgc
     1321 ggggcgcagg gcggccacgg cgcgggcctt ggcctcagcc agggccgcca ccagcgcctg
     1381 cggcgtgggc gcgctgacgg cgcgctcgtc cacctcgctg gcgacgacct cgaacctcgg
     1441 cgtgaccagc tgcagcagct cgcgcctgcg tgggctgccg gaagcaagaa tcagcatgaa
     1501 atgcgtctct ccttttgttt caacgatttt tgcccgggaa atgctacagg gcggcgggac
     1561 gggggaaaaa ggcaaaagaa aaagagggaa aggcggcggg cgctcagctg cgcccccctg
     1621 cggcccgaag ccgccccggg ccgccccaaa gctgcggccc cgcctttttc ctctctcttt
     1681 tcccctgtcc cttttccccg cgggccgcgc cggccctcac cgcacccggg tgcggttgta
     1741 gaggaacatc gccagcgcga tacagatgat ctgcgccacg ctgaccccga tggaaaagcc
     1801 gaaatagagc ttgaagacga tcaggtccag gcaggcgggg tcacccgccg caaagccgat
     1861 ggtgccgctg taggccagcc aggagaggaa tggtaccccg gcgcagaggt tcgacagcag
     1921 cgagcccacg atgatgccgg ccagcaggta gaacagaaag aaaaagttcc gtttcatccg
     1981 cctttccccc tcacagccgc ccggtggagc cgaagccgcc ctcgccgcgc tgggtctcgc
     2041 ccagctcctc cgcggccgca aaggccgcct gggccaccgg cagcaccgcc agctgggcga
     2101 tgcgctcgcc gttctgcacg gtgtaatcct cgtccgacag gttcaccagc cccacccgca
     2161 gctcgccccg gtagtcggag tcgatgaccc ccaccgagtt ggacagggtg atcccctttt
     2221 tgatgcccag gccgctgcgg gcaaagacca gcgccacata gcccgggccc ggcagctcga
     2281 tggcgatgcc ggtgggcagc acggcccgcc cgtgggcggg cagcagcacc ccctgcggcg
     2341 ggcagagggc gcacaggtcc gcggccgcgc tgccgggggt ggcccgctgc ggcagctttg
     2401 ccgccgggtc gagcagtttt actttcacgt tgcaatccat ctccatgcag tctcctttag
     2461 gcagtctctt ttttctgcgg ggcaggccgc cccgccggtc ttgagcgctt tcggcaggcc
     2521 cgggcgcgct ttttcccttg aacgccgttg aggccgtgcc ccgcgggtcc ttgcggcaag
     2581 ggcggcagga gcgccgtctg cgccggcggg cggcgctcca gccggccctc ttgacccggc
     2641 agctttttac tcgacggttt tatagtatag cccacgggtg aactccccgt caaagggcag
     2701 cccctgccgg tagagccgcg ccacctccgc ggcccgtgcg gcgctctcgc cggtgaagta
     2761 gagcacggtg aaatccgtgc ccacctcgcg cctgcggtcg cccagccaga gcggcaccgg
     2821 gttgagcagc tcgcgggcgg ggacggcgca ggccaccgtc agcctgcggc ccttgcggtc
//
LOCUS       DBMDYA010000366         2878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7667_length_2878_cov_1.326611, whole genome shotgun sequence.
ACCESSION   DBMDYA010000366 DBMDYA010000000
VERSION     DBMDYA010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7667_length_2878_cov_1.326611"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(154..1347)
                     /locus_tag="ACZPFK_09175"
     CDS             complement(154..1347)
                     /locus_tag="ACZPFK_09175"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR1406525.1"
                     /translation="MKLNNGLLSLEEDYVFSEVSRRVRRFASQNPDARLISLGIGDVR
                     GPICPAVTRAMRQAVADQARQKTFHGYGPEQGYQFLRQAIAAALTQRGAPVPPRDIFI
                     SDGAKTDLGGIFDLFAPGGAVLLCDPTYPAVRDDCRMRGLRPVSLPGTEANGFLPLPP
                     AGDGEVDAVYLCSPANPTGAVYDREQLAAWVEYARSHEALLIFDSAYESFITDPKLPR
                     SIYEIPGAKGCAVEIGSFSKVAGFTGTRCGYTVFPTELQVRGVSLQALWRRRLASKSN
                     GVAYVVQRGAAAVYSPSGRQQTARALGVYRQNSSRLAAALTAAGLPHWGGLHSPYLWL
                     KCPRGLSSWGFFDFLLQYGNLVGTPGIGFGPMGEGYFRLSAFGDPASVEQACDRLPRA
                     LEFLR"
     gene            complement(1593..2456)
                     /locus_tag="ACZPFK_09180"
     CDS             complement(1593..2456)
                     /locus_tag="ACZPFK_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1406526.1"
                     /translation="MTKKQALKAKLIWFLVLNAGLIFTAAGISIFKTPNHFAMGGTSG
                     LAIILADLFPRLNVGGAMMIINLVLVIFGFLFLGRSFGGATVYASAALSVYVELFEKL
                     IPLSSPLTDDTMLELIWAVILPAVGSALVFNIGASTGGTDIVAMVLAKYSSMEIGKAL
                     LVSDFLITAATFWLFDARTGLYCVLGLLLKTFLVDIVIDGINSRKYVTVICAEPEQVK
                     AFILEKLHRGATISQAEGAFTHRPEQVITTVLTRRQAVTLRNFIRGIDRHAFITMVNS
                     SEIIGKGFREI"
BASE COUNT          514 a          907 c         1013 g          444 t
ORIGIN      
        1 tttcggccat ccggcccgtc cactggccgg ggccatcgaa ataacggcga gatgcagaaa
       61 gcgcacccgg gccagtgctt tgaagcactg gcccgggcgt gtttttttgt gttttccagt
      121 gggcggcgcg cgagcggccg gggcggggcc gattcagcgc agaaattcca gtgcgcgggg
      181 cagccgatcg caggcctgct ccacgctggc cgggtcgccg aacgccgaca gacggaagta
      241 cccctcgccc atggggccaa agccgatgcc cggcgtgccc accaggttgc cgtattgcag
      301 caaaaagtca aagaaccccc aggaggacag cccgcgcggg catttgagcc acaggtaggg
      361 cgagtgcagg ccgccccagt ggggcaggcc agcggcggtg agggcggcgg ccaggcggct
      421 gctgttctgc cggtagacgc ccagggcgcg ggcggtctgc tggcggcccg aagggctgta
      481 gacggcggcc gcgccgcgct gcaccacata ggccaccccg ttggacttgg aggccagccg
      541 ccgccgccag agcgcctgca gcgagacccc gcgcacctgc agctcggtgg ggaagaccgt
      601 atagccgcag cgggtgccgg tgaagcccgc caccttggaa aaagagccga tctccaccgc
      661 gcagcccttg gctcccggga tctcgtagat gctgcggggc agcttggggt cggtgataaa
      721 ggattcatag gccgagtcga agatcaacag cgcctcgtgg ctgcgggcat actccaccca
      781 ggccgccagc tgctcccggt cgtagacggc gccggtgggg ttggccgggc tgcacaggta
      841 caccgcatcc acctcgccgt cgcccgcggg cggcaggggc aaaaagccgt tggcctcggt
      901 gccgggcagc gagaccgggc gcagcccccg catgcggcaa tcgtcccgca ccgccgggta
      961 ggtggggtcg cagagcagca ccgcgccgcc gggcgcgaac aggtcgaaaa tgccgccgag
     1021 atcggtcttg gccccatcgc tgataaagat gtcccgcggc ggcaccggcg cgccgcgctg
     1081 ggtcagggcg gcggcgatgg cctgccgcag gaactggtat ccctgctcgg ggccatagcc
     1141 gtgaaaggtc ttttgccggg cctggtcggc caccgcctgc cgcatggccc gggtcacggc
     1201 ggggcagatg gggccgcgca cgtccccgat gcccagcgag atcaggcggg cgtccgggtt
     1261 ttgtgaggca aagcgccgca cccggcggct gacctctgaa aacacataat cctcttccag
     1321 tgagagcaga ccgttgttca gcttcatcgc aaaaacccct gtgcagaaaa atcaggaaac
     1381 gcccgggcag cgccccgcgt ttcctgtatt tttacatccc tggttttcaa cggtcccttt
     1441 tatcgctctg tgggtcgctt tgcagcggca ggcggccccg gccgccagcc gtggcggagg
     1501 gcaaaaaaac ggcttgcccg aacggcggaa gcgagcgccc acggccgccc gcagccgcgg
     1561 gccgagcctg ccgggggaaa gcgaaaaccg gctcagatct cgcgaaagcc cttgccgatg
     1621 atctcggagg agttgaccat ggtgataaag gcgtggcggt cgatgccgcg gatgaagttg
     1681 cgcagcgtga ccgcctggcg gcgggtgagc acggtggtga tgacctgctc gggccgatgg
     1741 gtgaacgccc cctccgcctg gctgatggtg gcgccgcggt gcagcttttc caggataaag
     1801 gctttgacct gctccggctc ggcgcagatg acggtgacgt atttgcgcga gttgatgccg
     1861 tcgatgacga tgtccaccaa aaaggtcttc agcagcaggc ccagcacgca gtagagcccg
     1921 gtgcgggcgt cgaatagcca gaaggtggcg gcggtgatca aaaagtcgga caccagcagc
     1981 gccttgccga tctccatgga ggagtatttc gccagcacca tggccacgat gtcggtgccg
     2041 ccggtggagg cgccgatgtt gaagaccagc gccgagccca cggcgggcag gatgaccgcc
     2101 cagatcagct ccagcatggt gtcgtcggtg agcggcgaag acagcgggat cagcttttca
     2161 aagagctcca cgtagaccga cagcgccgcc gaggcgtaca ccgtggcccc gccgaagctg
     2221 cggcccaaaa agagaaagcc gaagatcacc agcaccaggt tgataatcat catcgccccg
     2281 cccacgttga gccgggggaa gaggtcggcc aggatgatgg ccaggccgct ggtgccgccc
     2341 atggcaaagt ggttgggcgt cttgaatata cttatgccgg ccgcggtaaa gattagaccg
     2401 gcgttgagga cgaggaacca gatcagcttt gccttgagag cctgcttttt tgtcatgaga
     2461 gcacttccct gtcggtagtt ggtggcggcg cagcggtccg cccggcgccg cccgtgctcc
     2521 attataaggc tttggccgac cggttgcaac aaaagacggc gttttctcgg tgagaaaacg
     2581 ccgtcgggta ttttgcctgc tttgtggggg gcgcgccgcg ggaaaaacgc cctttcagca
     2641 gcttttccgc cggtgcggaa aaacccggcg cgcggcggaa agagagggcg ggggcatggt
     2701 cgccggggac agcgcccctc gggaaacgcc tcaggcggcg ggggaggcgg tgggcgcggc
     2761 gttcagggcc gcgccgtcca cggtcaggct gtccgcgcgg gcgctgctga ccaccgcgat
     2821 atagcttttg cccgccgcga cggacagctc gccgccaccc gcgtcgtaga gtttcagc
//
LOCUS       DBMDYA010000367         2875 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7679_length_2875_cov_1.656813, whole genome shotgun sequence.
ACCESSION   DBMDYA010000367 DBMDYA010000000
VERSION     DBMDYA010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7679_length_2875_cov_1.656813"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="ACZPFK_09185"
     CDS             complement(<1..584)
                     /locus_tag="ACZPFK_09185"
                     /inference="COORDINATES: protein motif:HMM:NF021387.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR1406527.1"
                     /translation="MIDQVLLEERDGPLGEDKIARAVEASLADCRGSLGRVLLVVPDY
                     TRYHSGAGRIANLYYHLLAGSCRVDLLEALGTHVPMTRQQCADMYGDIPFERFIPHDW
                     RSDVVRLGEVPGSFVRQVSEGIMDQPIPVEVNRHLLDGYDLILSIGQVVPHEVVGMAN
                     YTKNILVGVGGSGMINSSHMLGAFYGLERMMGRD"
     gene            complement(598..2046)
                     /gene="gnd"
                     /locus_tag="ACZPFK_09190"
     CDS             complement(598..2046)
                     /gene="gnd"
                     /locus_tag="ACZPFK_09190"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HIR1406528.1"
                     /translation="MAKADIAMIGMAVMGENLALNMEDKGFSVAVYDIAEGVVDRFLA
                     GRGAGKRFTGAHSLPELVGALARPRKVMMMIRAGSPVDETIEKLIPLLEPGDIIIDGG
                     NSHFADTARRTALVEGKGLLYVGTGVSGGEMGALHGPSMMPGGSAAAWPQVRPIFHAI
                     CAKTDGAPCCEWVGSGGAGHFVKMVHNGIEYGDMQLICESYQLMRDLLGMSNEEMHQV
                     FKEWNEGELDSYLIEITRDILAYRDEDGGALVDRILDTAGQKGTGKWASIAALDEGVA
                     LTLIGEAVFARCLSAIKEERVRASALLHGPKTRFEGDRAALVWDIRDALYASKIVSYA
                     QGYTLMRTAAATYGWDLNFGEIALMWRGGCIIRSVFLGDIKRAFDADPGLTNLMLDRH
                     FCEVLQRAQAGWRRVCARAMECGVPVPAMSAALSYYDGYRCARLPANLLQAQRDYFGA
                     HTYERLDAPRGKFFHTNWTGEGGGTASSTYNA"
     gene            complement(2066..>2875)
                     /locus_tag="ACZPFK_09195"
     CDS             complement(2066..>2875)
                     /locus_tag="ACZPFK_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1406529.1"
                     /translation="VDQGGPWGYRHQFNIADSGYGMRAPRVLNDRAGEVGRGLSAADF
                     DLDRLFGEEGCRILHLSGLIAALSEEATQCCLEVARAAKKHGTILSFDMNYRASFWKG
                     RREELRRSFAEIASLADILIGNEEDFQLALGIEGPPAGGSGIQSKIDGFKGMIERVRA
                     AYPNASVFATTLREALSANSHEWGAVMLAEGEWHVEPLREIPVLDRIGGGDGFVSGML
                     YAILKGWEPEKWIQFGWATGALAATVLEDYGTPADEAQVWSIYKGNARVVR"
BASE COUNT          466 a         1045 c          882 g          482 t
ORIGIN      
        1 ttgtcccggc ccatcatccg ctccagcccg taaaacgccc ccagcatatg ggacgagttg
       61 atcataccgc tgccgcctac gcccaccagg atgtttttgg tgtagttcgc catgcccacc
      121 acctcgtggg gcaccacctg gccgatggag aggatcaggt cgtacccgtc cagcagatgc
      181 cggtttacct ccaccgggat gggctggtcc atgatgccct ctgatacctg ccgcacaaag
      241 ctgcccggca cctcgcccag ccgcaccacg tcgctgcgcc agtcgtgggg gatgaaccgc
      301 tcgaacggga tgtccccgta catgtccgcg cactgctgcc gcgtcatcgg cacatgggtg
      361 cccagggcct ccagcaggtc caccctgcag ctgccggcca gcaggtggta atagaggttg
      421 gcgatgcgcc ccgcgcccga gtgatagcgg gtgtaatcgg gcaccaccag cagcactcgg
      481 cccagactgc cgcggcagtc ggcaagggac gcctccaccg cccgggcgat cttatcctcg
      541 cccagcgggc cgtcccgttc ctccagcaat acctgatcga tcataccgcg cctccgtcta
      601 tgcgttgtag gtggaggagg ccgtgccgcc gccctcaccc gtccagttgg tgtggaaaaa
      661 tttgccccgc ggcgcgtcca gccgctcgta ggtatgggcg ccgaaatagt cgcgctgggc
      721 ctgcagcagg ttggcgggca gccgcgcaca gcggtagccg tcgtaatagc tgagggccgc
      781 gctcatggcg ggcaccggca cgccgcactc catcgcgcgg gcgcacaccc ggcgccagcc
      841 cgcctgcgcc cgctgcagca cctcgcaaaa atgcctgtcc agcatcagat tggtcaggcc
      901 cgggtccgcg tcaaaggcac gcttgatatc ccccaaaaag acgctgcgga tgatgcagcc
      961 gccgcgccac atgagggcga tctcgccgaa gttgaggtcc cagccgtagg tggcggcggc
     1021 ggtgcgcatc agggtgtacc cctgcgcgta ggagacgatc ttggaggcgt agagcgcatc
     1081 ccggatatcc cacaccagcg ccgcccgatc cccctcaaag cgggtcttgg ggccgtgcag
     1141 cagcgcggag gcgcgcaccc gctcctcctt gatggcggac aggcagcgcg cgaagaccgc
     1201 ctcgccgatc agggtcaggg ccaccccctc gtcgagcgcg gcgatagagg cccacttgcc
     1261 ggtgcccttt tgccccgcgg tgtccaggat cctgtccacc agcgccccgc cgtcctcgtc
     1321 ccgataggcc aggatgtccc gggtgatctc gatcaggtag ctgtccagct cgccctcgtt
     1381 ccactcttta aagacctggt gcatctcctc gttcgacatg cccagcaggt cgcgcatcag
     1441 ctgatagctc tcgcagatca gctgcatgtc gccgtactcg atgccgttgt gcaccatctt
     1501 cacaaaatgg cccgccccgc cggagcccac ccactcgcag cagggggcgc cgtcggtctt
     1561 ggcgcagatg gcgtggaaga tgggccgcac ctgcggccac gcggccgcag agccgccggg
     1621 catcatcgag gggccgtgca gggcccccat ctcgccgccg gagacaccgg tgcccacata
     1681 caacaggccc ttgccctcca ccagcgccgt gcggcgcgcg gtgtcggcaa aatggctgtt
     1741 gccgccgtcg atgatgatat cgcccggctc cagcaatggg atcagctttt cgatcgtctc
     1801 atccaccggg ctgccggcgc ggatcatcat catcaccttg cgcggccgcg ccagcgcgcc
     1861 gaccagctcc ggcaggctgt gggcgccggt gaagcgcttg ccggcgcccc gcccggcgag
     1921 aaagcggtcc accaccccct ctgcgatatc atagacggcc accgagaagc ccttgtcctc
     1981 catgttgagg gccaggttct cgcccatcac cgccatgccg atcatcgcaa tatccgcttt
     2041 tgccattcgt ctcttcccct ttcgcctatc tcaccacgcg ggcattgccc ttgtagatgc
     2101 tccatacctg ggcctcgtcg gcgggggtgc cgtaatcctc cagcacggtc gccgccagcg
     2161 cgccggtcgc ccagccgaac tgaatccact tttccggctc ccagcctttc agaatcgcgt
     2221 agagcatgcc gctgacaaag ccgtcgccgc cgccgatgcg gtccagcacc gggatttccc
     2281 gcagcggctc cacatgccat tcgccctcag ccagcataac ggcgccccac tcgtggctgt
     2341 tggcactcag ggcctcgcgc agggtggtgg caaaaacgct ggcgttgggg taagcggcgc
     2401 gcacccgctc gatcatgccc ttaaagccgt cgatcttgct ctggatgccg ctgccgccgg
     2461 cgggggggcc ctcgatgccc agcgccagct gaaaatcctc ctcgttgccg atgaggatat
     2521 cggccagaga ggcgatctcg gcaaagctgc ggcgcagctc ctcccggcgg cctttccaga
     2581 agctggcccg gtagttcatg tcgaacgaga ggatggtgcc gtgctttttc gcagcccgcg
     2641 ccacctccag gcagcactgg gtcgcctcct ccgagagggc ggcgatcagg ccggacagat
     2701 gcaggatgcg gcagccctcc tcgccgaaga gccggtccag gtcaaaatcc gctgcgctca
     2761 ggccgcggcc cacctcgccg gcgcggtcgt tgagcacccg gggcgcgcgc atgccgtagc
     2821 cgctgtcggc aatgttgaac tgatgccggt agccccacgg gccgccctgg tccac
//
LOCUS       DBMDYA010000368         2867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7710_length_2867_cov_1.270427, whole genome shotgun sequence.
ACCESSION   DBMDYA010000368 DBMDYA010000000
VERSION     DBMDYA010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7710_length_2867_cov_1.270427"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(99..2441)
                     /locus_tag="ACZPFK_09200"
     CDS             complement(99..2441)
                     /locus_tag="ACZPFK_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1406530.1"
                     /translation="MGDNGYLILDGGMGTMLQSAGLAGGQRPETLSVTEAQKITAIHR
                     AYLDAGAQVVYTNTFGANGLKAEGCGYSVQELVTAAVRAARAAAQGRARVALDIGPLG
                     ELLEPVGTLSFEEAYELFAQMVRPGAAAGADLVVIETMTDLYEVKAAVLAARENCALP
                     VWVSMTFEESGRTFTGCSVEAMAATLEGLGVDAVGINCSLGPVEILPIARRLCAATNL
                     PVFIKPNAGLPDPESGGYDITPEQFCAQMEPYLELGVFALGGCCGTTPEYIRRLAALC
                     RGRAPAPRPPVQASVVCTPTLVQRIDGLTVVGERINPTGKKLFKQALLDGNMDYILAQ
                     ALSQKEAGAQILDVNVGLPGIDEAAMMEQVVKNLQTVSDLPLQLDSSDPEALRRGLRV
                     YNGKPIVNSVNGEQKVLDAVLPLCKKYGAAVVGLTLDENGIPMLAQQRFAVAEKIVAA
                     CEAVGIPRRDIYIDCLTLTASAQQKEVVQTLAAIRMVKEQLGVRTVLGVSNISFGLPN
                     RELLNHSFLTMAAAAGLDLAIINPNVVSMMDAVRAADVLLARDKKAGRYLVAYANAAP
                     APSAATKREMTIEDAIYRGFKAEARQCTQELLLKMGEMRLVNERLIPALDVVGAEFEA
                     GKLFLPQLLQAASAAQAAFEVVKLSIASKGRPPVSKGKILVATVKGDIHDIGKNIVKV
                     ILENYGYDVIDLGRDVPAERVVKTAQEQGIRLVGLSALMTTTLASMEQTIRLLRETGV
                     ACRVFVGGAVLTPGYAKRIGADYYAKDAKQAVDIAKEVLG"
     gene            complement(2431..>2867)
                     /locus_tag="ACZPFK_09205"
     CDS             complement(2431..>2867)
                     /locus_tag="ACZPFK_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR1406531.1"
                     /translation="TAVTLGAGVDALLRAASAQDVARSVVLDAAASVAAEAAADAAEE
                     AAALALCRAGEYHTGRFSPGYGDFSITTQRAWVALLDAPRAIGLAATRAAILTPRKSV
                     TALIGVADHPVRGHLAGCDSCRLREKCELRKRGTPCGKADGR"
BASE COUNT          456 a         1053 c          870 g          488 t
ORIGIN      
        1 aaaaacggct gcgggcaagc ccgcagccat aatcaaaaat attccgtccc cggcgccttg
       61 cacgcgcaaa agcggccgcg cggttttggt ttaaaagcct atcccaacac ctctttggcg
      121 atgtccaccg cctgcttggc atccttggcg tagtagtcgg ccccgatgcg ctttgcgtag
      181 cccggcgtga gcaccgcacc gcccacgaac accctgcaag ccacccccgt ctcgcgcagc
      241 aggcggatgg tctgttccat gctggcgagg gtggtggtca tcagcgccga gagacccacc
      301 agccggatgc cctgctcctg cgccgtcttc accacccgct ccgcgggcac gtccctgccc
      361 aggtcgatca cgtcgtagcc atagttttca aggatcacct tgacgatatt tttgccgata
      421 tcgtggatat cccccttgac ggtggccacc aggatcttgc ccttgctcac cggaggacgg
      481 cccttggagg cgatggagag cttgaccacc tcaaaagcgg cctgggccgc cgaggccgcc
      541 tgcagcagct gcggcaaaaa cagctttccg gcctcgaact ccgcgcccac cacatccagc
      601 gccgggatca gccgctcgtt caccagccgc atctccccca tcttcagcag tagctcctgg
      661 gtgcactggc gcgcctccgc cttgaagccg cggtagatgg cgtcctcaat ggtcatctcc
      721 cgttttgttg ccgccgaggg cgccggcgcg gcgtttgcgt aggcgaccag atagcggccc
      781 gcttttttgt cccgggcaag cagcacgtcc gcggcccgca ccgcgtccat catagagacc
      841 acgttggggt tgatgatcgc cagatccagc ccggccgcgg ccgccatcgt caaaaagctg
      901 tggttgagca gctcccggtt gggcaggccg aaagagatgt tcgacacccc cagcacggtg
      961 cgcacgccca gctgctcctt gaccatgcgg attgccgcca gcgtctgcac cacctctttt
     1021 tgctgcgccg aggcggtgag ggtcaggcag tcgatataaa tatccctgcg cgggatgccg
     1081 acggcctcac aggcggcgac gatcttttcc gccaccgcaa accgctgctg cgccagcatg
     1141 gggataccgt tttcgtccag cgtcagcccc accaccgcgg ccccgtactt tttgcacagc
     1201 ggcagcacgg cgtccaacac cttttgctcg ccgttcaccg agttgacgat gggcttgccg
     1261 ttgtagacgc gcaggccgcg gcgcagcgcc tccgggtcgg aggaatccaa ctgcaggggc
     1321 aggtcgctca ccgtctgcag atttttcacc acctgctcca tcatcgccgc ctcgtcgatg
     1381 ccgggcaggc ccacgttgac atccaggatc tgtgcgcctg cctctttctg gctcagggcc
     1441 tgggccagga tgtaatccat attgccgtcc agcagcgcct gcttgaacag ctttttgccg
     1501 gtggggttga tgcgctcccc caccacggtc aggccgtcta tccgctgcac cagcgtgggg
     1561 gtacagacga cggaggcctg caccggcggc cggggggccg gcgccctgcc gcggcaaagc
     1621 gcggccaggc ggcggatata ctccggcgtg gtgccgcagc agccgcccag cgcaaaaacg
     1681 cccagctcca gatagggctc catctgggcg cagaactgct ctggtgtgat gtcgtaaccg
     1741 ccgctctccg ggtcgggcag gccggcgttc ggcttgatga agaccggcag gttggtggcc
     1801 gcgcacaggc gcctggcgat gggcaggatc tccaccggcc ccagcgagca gttgatgccc
     1861 accgcgtcca cgcccagccc ctccagcgtt gcggccatgg cctccacgct gcagccggta
     1921 aaggtgcggc ccgactcctc aaaggtcatc gacacccaga ccggcagcgc gcagttttcc
     1981 cttgcggcca gcaccgcggc tttcacctcg tacagatcgg tcatggtctc gatgaccacc
     2041 aggtccgccc ccgccgccgc gcccggacgc accatctggg caaacagctc atacgcctcc
     2101 tcaaacgaga gcgtccccac cggctccagc agctccccca gggggccgat gtccagcgcc
     2161 acgcgggcgc ggccctgcgc ggcggcgcgc gccgcccgca ccgcggcggt caccagctcc
     2221 tgcaccgagt agccgcatcc ctccgccttg aggccgtttg cgccgaacgt gttggtgtag
     2281 accacctgcg cgcccgcgtc cagataggcc cggtggatgg cggtgatctt ttgcgcctcg
     2341 gtgactgaga gcgtctccgg gcgctggccg ccggccaggc ccgcactctg cagcatggtg
     2401 cccatgccgc cgtccaaaat cagatatccg ttatcgccca tccgcttttc cacagggcgt
     2461 ccccctcttt ctcaattcgc atttttctct cagacggcag ctgtcgcagc cggcaagatg
     2521 cccgcgcacc gggtgatcgg caacgccgat cagcgcggtc accgatttgc gcggcgtgag
     2581 gatcgctgcc cgcgtcgcgg ccagcccgat ggcccggggc gcgtccagca gggccaccca
     2641 ggcccgctgc gtcgtgatgg aaaaatcccc atagccgggg ctgaaacggc ccgtgtggta
     2701 ctctcccgcc cggcacagcg caagggcggc cgcctcttcc gctgcgtcgg ccgcggcctc
     2761 cgccgccacc gaggccgcgg cgtccagcac caccgagcgc gccacgtcct gcgccgaggc
     2821 cgcccgcagc agggcgtcca caccggcgcc cagtgtgacc gccgtca
//
LOCUS       DBMDYA010000369         2863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7720_length_2863_cov_1.538740, whole genome shotgun sequence.
ACCESSION   DBMDYA010000369 DBMDYA010000000
VERSION     DBMDYA010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7720_length_2863_cov_1.538740"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="ACZPFK_09210"
     CDS             <1..513
                     /locus_tag="ACZPFK_09210"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1406532.1"
                     /translation="TALIEVCKARDEYFERTGIYVPVCSDGGIVHDYHVTLALAMGAD
                     FIMLGRYFSRFDESPTNKLNINGSYVKEYWGEGSNRARNWQRYDLGGGAKLSFEEGVD
                     SYVPYAGSLKDNVAQTLAKVRHTMCNCGALSIPELQQKAKLTLVSSTSIVEGGYHDVI
                     LKDKGLSSAE"
     gene            779..1285
                     /locus_tag="ACZPFK_09215"
     CDS             779..1285
                     /locus_tag="ACZPFK_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406533.1"
                     /translation="MFEVYKMMGTDFEGNAVLEPENPNDAYDRLQASLAVADEFQGYG
                     EEDDEDRVSFRELLELRYDLESRAYSKLKRELTPVRTYEAFASLEAFGCKINEKEIFD
                     YTDITEMEQDEPMDLEGGVEEAEHASYTSLLADAPVDDDPRYNELSLKKIREERDRML
                     LREKLKIK"
     gene            complement(1438..2202)
                     /locus_tag="ACZPFK_09220"
     CDS             complement(1438..2202)
                     /locus_tag="ACZPFK_09220"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HIR1406534.1"
                     /translation="MTAVQTTPGRRLGVLDAARGFVLLNMLAYHLLYDLVVLFGVRLD
                     WFVSWQGYVWQQFIACSFIFLSGVSCRFSHSNARRGAALLGWGVALSVATRLVIPGQF
                     IRFGVLHFLGCAALLWALMGRFLDRLPADFLFLASLLLFLFTNGTQWGYWGVWRVPVL
                     ELPDALYTTGWLFPLGFPGPDFYSADYFPLLPWLFLFWMGAAFHRLLEKAPRLLRRLS
                     PTVPLLGALGRRTLVVYLLHQPVLYGFVWLWYQLFL"
BASE COUNT          653 a          876 c          835 g          499 t
ORIGIN      
        1 acggcgctga tcgaggtctg caaggcccgc gacgagtact ttgagcgcac cggcatctat
       61 gtgccggtct gcagcgacgg cggcatcgtc catgactatc acgtcaccct ggccctggcc
      121 atgggcgccg atttcatcat gctgggccgc tacttctccc gctttgacga gagccccacc
      181 aacaagctga acatcaacgg cagctacgtc aaggagtact ggggcgaggg ctcgaaccgc
      241 gcccgcaact ggcagcgcta cgacctgggc ggcggcgcca agctgtcgtt tgaggagggg
      301 gtggacagct atgtccccta cgccggcagc ttgaaagaca acgtggccca gacgctggcc
      361 aaggtgcgcc acaccatgtg caactgcggc gccctctcca tccccgagct gcagcagaaa
      421 gccaagctca cgctggtttc ctccaccagc atcgtggagg gcggttacca cgacgttatt
      481 ctcaaggaca agggcctctc ctccgcggaa taaaacgctt cctgctttgt gaaaaacagc
      541 ggctgtatcg cagccgctgt ttttttggtg catttttgta aaatttgttc aaatttgttc
      601 tttcggtttt aaccaatatg gatattttac ctaaaatcta ttgtttttaa gcgcttccag
      661 aggtataata tggttgtaaa ataagacagg ggaaatgaga ccggtgctta ttttgcgaaa
      721 gcgttttccg gcagcccgcc gcggctgcgc ccccctgtct ggaatagagg taatcgcgat
      781 gtttgaggtt tacaagatga tggggactga ctttgaaggc aacgcggtgc tggagcccga
      841 aaacccgaat gacgcttatg accgcctgca ggccagtctg gcggtggcgg atgaatttca
      901 ggggtacggc gaagaggacg acgaggaccg cgtctctttc cgtgagctgc tcgagttgcg
      961 gtacgacctg gagtcccgcg cctactcaaa gctcaagcgc gagctcacgc cggtgcgcac
     1021 ctacgaggcc tttgcctctc tggaggcgtt cggctgcaag atcaacgaaa aagagatttt
     1081 cgactacacc gacatcaccg agatggagca ggatgagccc atggatttgg agggcggcgt
     1141 ggaggaggct gagcatgcca gctacacctc tctgctggcc gacgcgccgg tggacgacga
     1201 tccgcgctac aacgagctct cactgaaaaa gatccgtgaa gagcgcgacc gcatgctgct
     1261 gcgggaaaag ctgaagatca aataaggcgg gcaaaagcac ggcaaaaaat caaaaggcgg
     1321 gcgctttggt ggcaaagcgc ccgccttttt tgcccgtgaa agccgatgga tatcgtctgc
     1381 gggcctcacc gtgcgaaccc caccgtgcgg gaggcggggc cgccgcgcgg ccttttctca
     1441 cagaaaaagt tggtaccaca gccagacaaa accgtagagc accggctggt gcagcagata
     1501 gacgacgagg gtgcggcgcc ccagcgcgcc cagcagcggc accgtcggcg acaggcggcg
     1561 cagcaggcgg ggcgcttttt ccagcaggcg gtggaacgcc gcccccatcc agaacaaaaa
     1621 aagccagggc aacagcggga aataatcggc cgagtaaaaa tccggccccg gaaaccccag
     1681 cggaaaaagc cagccagtgg tgtacagggc atcgggcagc tccagcaccg gcacccgcca
     1741 aacaccccaa tagccccact gggtgccgtt tgtaaacagg aacagcagca ggctggcgag
     1801 aaaaaggaaa tctgccggga gccggtccag aaagcggccc atcagggccc aaagaagggc
     1861 tgcgcagccg agaaaatgca gcacgccaaa gcggatgaac tgccccggga tcaccagcct
     1921 ggtggcgacg ctgagcgcca cgccccaccc cagcagcgcc gcaccgcggc gcgcattact
     1981 gtggctgaag cggcaggaga cgcccgacaa aaagataaag ctgcaggcga tgaactgctg
     2041 ccagacatac ccctgccagc tcacgaacca atccaggcgg acgccgaaca gcaccaccag
     2101 atcatagaga agatgatagg ccagcatatt gagcagcaca aagccgcgcg ccgcatccaa
     2161 cacgcccaat cgtcggccgg gtgtggtctg tacagcggtc aacttgtccc cctcctctcc
     2221 ctgcttcgcg ctttttattt ctttggcaat caaagccgca gacagcggcg gtatcgcctc
     2281 tccggcacgg ggatgaaaac cggcgagcga aaagagctgc tgaaacgcgg agagagcaga
     2341 ggtccgggcg gccgcgcgcc gcgccgctta tcctgtcggt gcggtagcac cacatgcttc
     2401 ctcttgccgg ggaggcagca ggatgtgcgc gccttgcagc tctcgcaaag caggctttcc
     2461 ctgctctttt catcgaattt tctttccgcg tttttcaccc ggctctcgcc cggctcaagg
     2521 cggccgccgc atggctcccc tttttcgagg cgcggtcgcg caagggacgg agggggcaac
     2581 cgatgcggaa aacgcaactt atccccaccg gccttttccg ccctccccgg gacaacggca
     2641 agccacgcca cacgatcccc aaaacggttt atgacggccg tggcccgggg acgggccgca
     2701 tggccataaa aacagccatt tcaaatgacc ccgtttctcg ccgctgcgcg gcaaccgaaa
     2761 cggatggccg taaaaatagc ctccggctat ttttataacg caagagccag gcattccctt
     2821 tcacttcagc aaaaaatttt acattccccc cccccgcgtc tgc
//
LOCUS       DBMDYA010000370         2862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7727_length_2862_cov_1.448750, whole genome shotgun sequence.
ACCESSION   DBMDYA010000370 DBMDYA010000000
VERSION     DBMDYA010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7727_length_2862_cov_1.448750"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="ACZPFK_09225"
     CDS             complement(<1..502)
                     /locus_tag="ACZPFK_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1406535.1"
                     /translation="MQQQLNVTNKTRRLTTMALLAALAVILVALIHFPLLPAAPFLEY
                     DPADIAIFIGAFLFGPLPGLALTVVTSVIQGVTVSAGSSWIGIVMHILATGSYVLVAG
                     AIYQRRHSRRGAVLALAAGTLTMTVVMCGCNLIFTPLFMGQSVQDVVAMLLPVIAPFN
                     LLKAGIN"
     regulatory      complement(687..827)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1076..2164
                     /locus_tag="ACZPFK_09230"
     CDS             1076..2164
                     /locus_tag="ACZPFK_09230"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1406536.1"
                     /translation="MDDVSSVIARLTGSENPLWQWALCAVVFFVFLLLSRLYRYKAVA
                     VILKFAKRLRGTSWEELLRALSRPVAALLLLVGATFALWNLPLSAAVMAPLRAFLTKA
                     LRLVSTGLIGWAGCGIVDTVPVFEFKFLGSAEAVRRMLRRVVKVLIAAFAALAMLEIL
                     GFPVGSLIAGLGLGGLTVSLAAKDTASNLFAGLVLLVEKPFAIGDWVTCAGVEGAVED
                     ITFRSTKIRTLANTLTVVPNSVVSAGLISNGSERKMRMAEFTIGVCYDTPRAALEQVM
                     ADLRALLTGDAAVRADTVVVRFTGFSASSMDVLVRYYTKTTDYNEFLAVGERLNLAIL
                     DLMASNGVSFAFPSTTVYLEDAGKGPAQ"
     gene            complement(2404..>2862)
                     /locus_tag="ACZPFK_09235"
     CDS             complement(2404..>2862)
                     /locus_tag="ACZPFK_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016159.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIR1406537.1"
                     /translation="LFTKGILKCTSGVSAGETVELIRSIAETVAETYGVDKTAAVTRA
                     KAGIAEHLDENENLAPFDLGVEVFAGNEEMEQEFRRQASAAGVPERVSVPKKLAVRTA
                     RSQKIKTDTGIEIIFPLDYAENTDFLEFVHNPDGTIAIEIKNVGSIENRG"
BASE COUNT          489 a          913 c          896 g          564 t
ORIGIN      
        1 tgttgatgcc cgccttgagc aggttgaacg gggcgatcac cggcagcagc atggcgacca
       61 cgtcctgcac cgactggccc ataaacagcg gggtgaagat cagattacag ccgcacatca
      121 ccaccgtcat ggtcaaggta cccgcggcga gggccagcac cgcgccgcgg cggctgtggc
      181 ggcgctgata gatggccccc gccaccagta cataagagcc ggtggcaagg atgtgcatga
      241 caatgccgat ccagctgctg cccgcgctga ccgtgacccc ctggatgacg ctggtcacca
      301 ccgtcagggc caggccgggc agcggcccaa agaggaacgc cccgataaaa atggcgatgt
      361 cggccgggtc gtactcgaga aaaggcgcgg ccggcagaag cggaaaatgg atgagggcca
      421 ccaaaatgac cgccagggcg gccagcaaag ccatggtggt gagcctgcgg gtcttgtttg
      481 tgacgttgag ctgttgttgc atgagagatg cctcctttaa aatgttggtc tgccgacctg
      541 cgcggcaccg cgcgccggtc cgggaggctg tgccggtccc catccgcctt tgcgctttgg
      601 cccgacgccc tgtgcgggga gcaaagccac aaaaaaagcc ccttttgcaa ttgtttgcaa
      661 aaagggcgca ttgcgaatgg aaacagccgc agtttctttc atccggactt tgaccgtcgg
      721 ctctggaatt tcaccagatc agcgcctttc accgggaaaa gcggtcgtgg gcttggccct
      781 tgggcctcac caccggtggg gactttcacc ccgccctgaa acagactgta aatagtatag
      841 tgcgccgctc tattttttgc aactgccggc agagtgaaaa aatgccgttt tccggcgctc
      901 tgcccgcccg ctgtccgccg cgcgggcgga gacgggtatt ttctcgcccc cgctttcttt
      961 tcggcccttt ttccctgggc cggcccccgc cgatcaggcc ggttgcgcgc cgccgcccgg
     1021 tatggtacta ttctaaaagg acaaaatatc ccataaaatg aaaggagagg gacgcatgga
     1081 cgacgtatcc tccgtcatcg cgcggctgac cggcagcgag aacccgctct ggcaatgggc
     1141 gctctgcgcc gtggtgtttt tcgtttttct gctgctcagc cgcctttatc ggtataaggc
     1201 ggtcgctgtg atcctgaaat ttgcaaaaag gctgcgcggc acctcctggg aggagctgct
     1261 gcgcgcgctc tcgcgcccgg tggccgccct gctgctgctg gtgggggcca cctttgcgct
     1321 gtggaacctg cccctctccg cggcggtgat ggccccgctg cgcgcctttc tcaccaaagc
     1381 gctgcggctg gtctctaccg ggctgatcgg ctgggccggc tgcggcatcg tggacacggt
     1441 gccggtcttt gagttcaaat ttctcggctc tgccgaggcg gtgcggcgca tgctgcgccg
     1501 ggtggtgaaa gtgctgatcg ccgcctttgc ggcgctggcg atgctggaga tcctgggctt
     1561 cccggtgggc agcctgatcg ccgggctggg ccttggcggc ctgaccgtct cgctggccgc
     1621 caaggacacc gcctccaacc tgtttgccgg gctggtgctg ctggtggaaa agccctttgc
     1681 catcggcgac tgggtcacct gtgccggggt ggagggggcg gtggaggaca tcaccttccg
     1741 cagcaccaaa atacgcaccc tcgccaacac cctgaccgtg gtgccgaact cggtggtcag
     1801 cgccgggctg atctcaaacg gcagcgagcg caagatgcgc atggccgaat ttacgatcgg
     1861 cgtctgctac gacacaccgc gcgccgcgct ggagcaggta atggccgacc tgcgcgccct
     1921 gctcaccggc gacgcggcgg tgcgcgcaga cacggtggtg gtgcgcttta ccggcttttc
     1981 ggccagcagc atggacgtgc tggtgcgcta ttacaccaag accaccgact acaacgagtt
     2041 tttggccgtc ggcgagcggc tgaacctggc gattttggac ctgatggcga gcaacggcgt
     2101 ctcgttcgct tttccctcca ccaccgtcta tctggaggat gcgggaaaag gcccggctca
     2161 ataaatccgc agcggtcaag ccgcgggcat cccgcgccgc cctgacaggc gccctttcca
     2221 ctttaaaaaa gcaaagcgcg gtattggaac aaagcccggg tccatcctga gatggacccg
     2281 ggctttgttc ttcaaaattt taaggccccg gggccgctgt gggtcaaggc ccttcacaca
     2341 ggccattgtg agctccacat cgccttgggg cctcggggct gccgcggggc gggtccgccc
     2401 gccctatccc cggttctcga tgctgcccac gtttttgatc tcgatggcaa tggtgccgtc
     2461 cgggttgtgg acaaactcca aaaagtcggt gttctctgcg taatccagcg gaaagatgat
     2521 ctcgatgccg gtgtcggtct tgatcttttg gctgcgggcc gtgcgcaccg cgagcttttt
     2581 gggcaccgag acccgctccg gtacgccggc tgcactggcc tgccggcgga actcctgctc
     2641 catctcctca ttgcctgcaa acacctccac gcccaggtca aagggcgcca ggttctcgtt
     2701 ctcgtccaga tgctccgcga tgcccgcctt ggcccgggtc accgccgcgg tcttgtccac
     2761 cccataggtc tcggccaccg tctcggcgat cgagcggatc agctccaccg tctcccccgc
     2821 cgagacgccg gaggtacatt tgaggatacc cttggtgaaa ag
//
LOCUS       DBMDYA010000371         2861 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7733_length_2861_cov_1.534058, whole genome shotgun sequence.
ACCESSION   DBMDYA010000371 DBMDYA010000000
VERSION     DBMDYA010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7733_length_2861_cov_1.534058"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /gene="ftsH"
                     /locus_tag="ACZPFK_09240"
     CDS             complement(<1..1693)
                     /gene="ftsH"
                     /locus_tag="ACZPFK_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1406538.1"
                     /translation="MPTSTMNMKYSQVLGYFKEQKVKSFTFDPNDGTLTLVMSAEDAE
                     AALKETQKLNGVTQVVAIGGFGSQQGQGQAPGADKDGQPSATAAPKDQELTYTVAYAN
                     LFLNQVMPYIDAYDEAHPDAPMVYDILPVQSTPMWLSFLPTIIMVVALGALFWMMMRQ
                     SGGGGGVMNVGKVKAKDQAAEGRKATFADVAGADEEKAELQEIVEFLKSPAKFNALGA
                     RIPRGVLLVGPPGTGKTLIARACAGEAGVPFYSISGSDFVEMYVGVGASRVRDLFEKA
                     KKSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFTANDGVIVIAATN
                     RADILDKALLRPGRFDRQIYVGLPDVRGREQILKVHTRNKPMAPDINLATIAKSTPGF
                     TGADLENLVNEAALLAARRGKKAITQSEIEEASIKVIAGPEKKSRVITPKERRLTAYH
                     EGGHAIATYYSKTGDPVHQITIVPRGAAGGFTMSLPEEDTNYTTKSQMEDEIVILLGG
                     RVAEKLVLDDISTGASNDIERASEIARHMVTRYGMSDKLGPITYGSSHDEVFLGRD"
     gene            complement(1877..2416)
                     /gene="hpt"
                     /locus_tag="ACZPFK_09245"
     CDS             complement(1877..2416)
                     /gene="hpt"
                     /locus_tag="ACZPFK_09245"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1406539.1"
                     /translation="MKDDVKKVLLSEEELHAKVAELGAQITKDYEGKNLLLVTVLKGA
                     VVFLADLMRTIDQPAEIDFMVVSSYGSGVKSSGVVKIVKDLDINLEGKDILIVEDILD
                     SGMTLDYIKGMLQDRNPSSIRICALLDKPARRKVDLQADYVGFTIPDEFVIGYGLDYD
                     EKYRNLPYIGVLKPEVYSV"
     gene            complement(2423..2680)
                     /gene="tilS"
                     /locus_tag="ACZPFK_09250"
     CDS             complement(2423..2680)
                     /gene="tilS"
                     /locus_tag="ACZPFK_09250"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1406540.1"
                     /translation="MINKISTFRTRRPGDLFRPAGRGVTKSLKKLLAEDGVPRGSRDR
                     LLVLAEGERVLWLEGVGVCEELLPRPGCRRAVQIKLIEPEE"
BASE COUNT          497 a          924 c          841 g          599 t
ORIGIN      
        1 agtccctgcc caggaacact tcgtcgtggc tgctgccata ggtgatgggg cccagcttat
       61 cgctcatgcc gtagcgggtc accatgtggc gggcgatttc agaagcccgc tcgatgtcgt
      121 tggaagcgcc ggtggagata tcgtccagca ccagcttttc cgccacgcgg ccgcccaaga
      181 ggatgacgat ttcatcctcc atctggctct tggtggtgta gttggtgtcc tcctcgggca
      241 ggctcatggt aaagccgccc gcagcgccgc ggggcacgat ggtgatctgg tgcaccgggt
      301 cgccggtctt ggagtaatag gtcgcgatgg cgtgaccgcc ctcgtgatag gcggtgagcc
      361 gccgctcttt gggggtgatg acccgactct ttttctcggg gccggcgatg accttgatgc
      421 tggcctcctc gatctccgac tgggtgatgg cctttttgcc gcgccgcgcc gcaagcagcg
      481 ccgcctcgtt gaccagattt tccaggtcgg cgccggtaaa gccgggcgtg gatttggcga
      541 tggtggcgag gttgatgtcc ggcgccatcg gcttgttgcg ggtgtgcact ttcaaaatct
      601 gttcgcggcc gcgcacatcg ggcagcccca catagatctg acggtcaaaa cggcccgggc
      661 gcagcagcgc tttgtccagg atatccgccc ggttggtggc cgcgatgacg atgacgccgt
      721 cgttggcggt aaagccgtcc atctcgacca acagctggtt cagggtctgc tcgcgctcat
      781 cgtgaccgcc gcccagaccc gcgccgcgct ggcggcccac cgcgtcgatc tcgtcgataa
      841 agatgataca gggcgcgctc tttttggctt tttcaaacag gtcccgcacg cgagaagcgc
      901 cgacgcccac atacatctcc acaaagtcgg agccggagat ggagtagaac ggcacgccgg
      961 cctcgcccgc gcaggctcgg gcgatcagcg tcttgccggt gcccggaggg cccaccagca
     1021 gcacgccgcg cgggatgcgc gcgcccagcg cgttgaattt tgccgggctc ttgaggaact
     1081 ccacgatctc ctgcagctcc gccttttcct catccgcgcc cgccacgtcg gcaaaggtgg
     1141 cctttcggcc ctcggccgcc tgatccttgg ccttgacctt gcccacgttc atcacgccgc
     1201 caccgccgcc ggactggcgc atcatcatcc agaacagcgc gcccagcgcc accaccatga
     1261 taatggtggg caaaaagctg agccacatgg gggtggactg caccggcagg atgtcataga
     1321 ccatcggcgc gtcgggatgc gcctcgtcgt aggcgtcgat atagggcatc acctggttca
     1381 ggaagaggtt ggcgtaggcc accgtatagg tgagctcctg atccttgggc gcggcggttg
     1441 ccgacggttg gccgtccttg tccgcgccgg gggcctggcc ctgcccttgt tgcgagccaa
     1501 agcccccgat ggcgaccacc tgggtcaccc cgttgagctt ttgggtctcc ttgagcgcag
     1561 cctcggcgtc ctcggcggac atgaccagcg tcaaagtgcc gtcgttgggg tcaaaggtga
     1621 agcttttgac tttctgctct ttgaaatacc ccagcacctg ggaatatttc atgttcatgg
     1681 tggaggtggg catccggaag atcaaaaaga ccagcaccgc cgccagcgcg agcaggatga
     1741 caaagcttgc cgtacttctg tttggttttt tgctcaaatt cgtttcactc caactgccgc
     1801 ctgccggcgg ctgtaaaaat agccgttttt atcgcccgtc ccgctcaccc gcggagcagg
     1861 cccggccgaa agccgctcag accgagtaga cctccggctt gagcaccccg atatagggca
     1921 ggttgcggta tttctcgtcg taatccaggc cgtaaccgat gacgaactcg tcggggatgg
     1981 tgaagcccac atagtccgcc tgcaggtcca ccttgcggcg ggccggcttg tcgagcagcg
     2041 cgcagatgcg gatcgaagag gggttgcggt cctgcagcat gcccttgata taatccagcg
     2101 tcatgccgct gtcgaggata tcctccacga tcaggatgtc cttgccctcc aggttgatgt
     2161 ccaggtcttt gactatttta accacgccgc tgctttttac accggagcca taagaggaga
     2221 ccaccataaa atcaatctcc gccggctgat cgatggtgcg catcaggtcc gccagaaata
     2281 ccacggcccc cttgagcacc gtcacgagca gcaggttttt gccctcgtaa tctttcgtga
     2341 tctgcgcgcc cagttccgca accttggcgt gcagctcctc ctcgctgagc aatacctttt
     2401 tgacgtcgtc tttcatcatt tttcattcct ccggttctat caacttgatc tgtaccgccc
     2461 gccggcagcc cgggcgcggc agcagctcct cacacacgcc gactccctcc agccacagga
     2521 ccctctcgcc ctccgccagc accagcaggc ggtcgcggga gccgcggggg acgccgtcct
     2581 ccgccagcag ctttttgagg gatttcgtca ccccgcgccc ggccggacgg aaaaggtccc
     2641 ccggccgccg ggtacggaaa gtggaaatct tatttatcat accataatct gcaaattttt
     2701 ttaactcttt tttatccccg ccgcaaagtt ttacgatttc ttcatactct tgttccagaa
     2761 cggccacggt ccgcccgtcc ggcagcgcag tctgtcccgt agcaaagggg accgcccacc
     2821 tcgccgcggg cacgggagcg tccctctcaa gataaaagac g
//
LOCUS       DBMDYA010000372         2859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7740_length_2859_cov_1.195794, whole genome shotgun sequence.
ACCESSION   DBMDYA010000372 DBMDYA010000000
VERSION     DBMDYA010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7740_length_2859_cov_1.195794"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            320..1297
                     /locus_tag="ACZPFK_09255"
     CDS             320..1297
                     /locus_tag="ACZPFK_09255"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406541.1"
                     /translation="MKKIILDCDPGHDDAFAIMLAARHLEILGVTTVGGNGYLPNVTR
                     NALQVLDKIGRADIPVYAGHAGPSTLPLVIAPNVHSESGMDGPSLPPPSRPAEDRHAV
                     DFIVETVMSTADVTLVATGPLTNIAAALNREPRIAQRAQALYLMGGGAYCGNWTPAAE
                     FNIWVDAEASYKVFHSGLPLYMAGVNLTRQCPVLAEDIEAMRGFGNKTGRFAAELLDF
                     FAHGGDAHLHDPCAVAWLIDPSLVRSAFLHVDIELNGTLTRGMTVTDLRPLQTQDLER
                     DIDLDSPHRWDDAPEGSPFRGSAPNTHVGMRLDFAGFRRLLLDTIRSYD"
     gene            complement(1495..2778)
                     /locus_tag="ACZPFK_09260"
     CDS             complement(1495..2778)
                     /locus_tag="ACZPFK_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1406542.1"
                     /translation="MGTPGPSMLVALVLLLAFSAFFSASETAISSINKIRVKNRAEEG
                     DKKAAVALKNAEDYDKTLSAILIGNNVVNLTLSSIATLVATALLGDAGAAVATVVTTV
                     LVLIFGEILPKSFAKDNAEQVAFATARPLSWIKFLFTPLVWLFVQIKHIFTGRRSSEL
                     NIQPSVTEDELKTIVDTVEDEGVLSPQETGIIQSAIDFDDTTVQEILVPRVDMLAVDV
                     DAPLEEVLSACVDEGFSRIPVYEGTIDNVVGILYAKDVLECLVKQRPVAVRTLMRPVL
                     YVYRTKHINALLAEFRREKQHMAVVTDDYGGVMGLVTMEDILEELVGEIWDETDREEP
                     PVRRLSDRCWEVAGEQNVEDFFEEIGFEDRDFTCGYSTVAGWALEVLEHIPAEGETFR
                     YKDLKVTVEKMEEQRIAQLRVERAAPPAPEQEVGT"
BASE COUNT          506 a          911 c          906 g          536 t
ORIGIN      
        1 cgggacgacc acctaccggc tgggggtact ggcccgggac gacgcgctgg gccgctacat
       61 cgccctgcag ctggagccgg gcgcggtgac gggcagggag ctggaagaga tcgcccgcag
      121 cgtggagttt gggcgctgaa aaaatttgcc cgcgcgcgat gcgccgccgg ggggcataag
      181 gccctcccgg cggcgcatcg ctgttttcgg cgctttacag cggcgcggga aaacagtaca
      241 atggtaaaaa acggaagctc ccgcatccgc ccgcgcccgc ttcggcgctg cgggcgcgga
      301 tgccaaaagg agagggaaaa tgaaaaaaat cattctcgac tgcgatcctg gccacgacga
      361 cgcctttgcc atcatgctgg cggccaggca tctggagatc ctgggggtca ctacggtggg
      421 cggcaacggc tacctgccca acgtcacccg caatgcgctg caggtgctgg acaagatcgg
      481 ccgcgcggat atcccggtct atgccggcca cgcgggcccc tccacgctgc ctctggtgat
      541 cgcccccaac gtccacagcg agagcggtat ggacggcccc agcctgccgc cgcccagccg
      601 cccggcggag gaccggcacg cggtggattt tattgtggag acggtgatgt ccaccgcgga
      661 cgtgacgctg gtggcgaccg gcccgctcac caacattgcg gcggccttga accgtgagcc
      721 gcgcatcgcc caaagggcgc aggcgctcta tctgatgggc ggcggcgcct attgcggcaa
      781 ctggaccccc gcggcggagt tcaacatctg ggtggacgcg gaggcgtcct ataaggtctt
      841 tcacagcggc ctgcccctct acatggcggg cgtcaacctg acccgccagt gcccggtgct
      901 ggcagaggat atcgaggcca tgcgcggctt cggcaataag accggccgct ttgcggctga
      961 gctgctggat ttttttgccc acggcgggga tgcgcatctc cacgacccct gcgccgtggc
     1021 ctggctcatc gatccctcgc tggtgcgctc ggcttttctg catgtggaca tcgagttgaa
     1081 cggcaccctg acgcggggca tgacggtgac tgatctgcgc ccgctgcaga cccaggacct
     1141 tgagcgggat atcgatctcg attctcccca ccgctgggac gacgcgccgg agggatcgcc
     1201 tttccgcggc agcgcgccca acacccatgt gggcatgcgg ctggattttg cggggttccg
     1261 ccgtctgctg ctggacacca tccgcagcta tgattgaatc gccctgcaag aacgatctcc
     1321 aacgcagaga gataaaaata aaaaagacgg gggacacggc tcggccgcgt ctctcgtctt
     1381 ttttatctaa aaaaccgccg gctcagccgg cggtttttgt tttatcgctt gccggggctc
     1441 tgggagggag gtccggtggc gcgcgccccg tgtcgctctg ggggcagagc gggttcatgt
     1501 gccgacctcc tgttccggtg cggggggcgc agcccgttcc acccgcaact gggcgatccg
     1561 ctgctcttcc attttttcca cggtgacttt caggtctttg tagcggaacg tctccccctc
     1621 ggcggggatg tgctccagca cctccagcgc ccagcccgcc acagtggaat agccgcaggt
     1681 aaagtcccgg tcctcaaagc cgatctcctc gaaaaagtcc tccacattct gctcgcccgc
     1741 cacctcccag cagcggtcgg agaggcggcg caccggcggc tcttcccggt cggtctcgtc
     1801 ccagatttcg cccaccagct cttccagaat gtcctccatg gtcaccagcc ccatcacgcc
     1861 gccgtagtcg tcggtgacga cggccatgtg ctgcttttcg cgccgaaact ccgccaacag
     1921 ggcgttgatg tgtttggtgc ggtagacgta cagcacgggc cgcatcagtg tacgcaccgc
     1981 gacagggcgc tgcttgacaa ggcactccag cacgtccttg gcgtagagga tgcccaccac
     2041 gttgtcgatg gtgccctcgt agaccgggat gcggctgaag ccctcgtcca cacaggcgga
     2101 cagcacctct tccagcgggg cgtccacgtc cacggccagc atgtccacac ggggcaccaa
     2161 aatctcctgc accgtcgtgt cgtcaaagtc gatggccgac tggatgatgc ccgtctcctg
     2221 cgggctcagc acgccctcgt cctcaacggt gtccacgatg gttttcagct catcctccgt
     2281 cacggagggc tggatgttca actcgctgct gcgtctgccg gtgaagatgt gcttgatctg
     2341 cacgaacagc cagaccagcg gggtgaaaag aaacttgatc caggacagcg ggcgcgcggt
     2401 ggcaaaggcc acctgctcgg cgttgtcctt ggcaaagctc ttgggcagaa tctcaccgaa
     2461 gatcagcacc agcacggtgg tcacaacggt ggcgaccgcc gcccccgcat cccccagcag
     2521 cgccgtggcc accagggtgg cgatggagga aagggtcagg ttgaccacgt tgttgccgat
     2581 caggatggcg gagagggtct tgtcgtagtc ctcggcgttt ttcagcgcca cggcggcttt
     2641 tttgtcgccc tcctcggccc ggtttttgac ccggatcttg ttgatggagg agatggctgt
     2701 ctcgctggct gagaaaaagg cggagaacgc cagaagcagc accagggcga cgagcatact
     2761 gggaccaggg gtgcccaaat gaaatcaact caactttctg ttttatactg tatttgaaac
     2821 gatctatcgg atgttctgct ttgtttgaga ggcggaggg
//
LOCUS       DBMDYA010000373         2858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7743_length_2858_cov_1.585419, whole genome shotgun sequence.
ACCESSION   DBMDYA010000373 DBMDYA010000000
VERSION     DBMDYA010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7743_length_2858_cov_1.585419"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="ACZPFK_09265"
     CDS             complement(<1..373)
                     /locus_tag="ACZPFK_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406543.1"
                     /translation="MKKTGPFHYAWVILGCCMLMAFAGMGVITSACGNFVTPVVEELG
                     CSVSSFTMFISIQAASMALLYTTASRVLTTRPIGKVVACALLLQFVGMALMGSYHSIG
                     WFYLSGLLTGIGSAFALYIALP"
     gene            complement(488..1084)
                     /locus_tag="ACZPFK_09270"
     CDS             complement(488..1084)
                     /locus_tag="ACZPFK_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HIR1406544.1"
                     /translation="MACREPEYLYCLAAYPEGEAGLPFEAQAILCGPAAAAIWHPVDY
                     SFEWMSRYRCPANAVSDLLGLAPADQVKYFRRIIRHGGRPLACEMSWRPDSACKKPLD
                     LTLEAPAIRCVLDGFLVEQCVRYRAMSHKHSQQLEIPQDTMVICLESLVCSRDGNVPL
                     QLSRLFIAPSRGYVTAVSPRQPPRRASEPPGLSFYDPC"
     gene            complement(1093..1824)
                     /locus_tag="ACZPFK_09275"
     CDS             complement(1093..1824)
                     /locus_tag="ACZPFK_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406545.1"
                     /translation="MKNTYRANKRALTCEKIRGYIREQGLRPGSKLPSQREMCALFGV
                     SRQCIHDAVAILVREGTLYTRPRSGQYVGAWPFEVLTTSAASNLRRHQYPNFVVLSTQ
                     TLSQRKILPSGYLRQVMELSADAPVLELKRLRRREGAFMCLEYSYTPLDLFPDFLHTD
                     FNRESIRKKFQELDLEPFVSRSIICISALAAEDAALLGARGNVAVSSGIVWSQNRQRV
                     IEYYRNYSDLRSIVYTLEAGRKEGG"
     gene            complement(1821..>2858)
                     /locus_tag="ACZPFK_09280"
     CDS             complement(1821..>2858)
                     /locus_tag="ACZPFK_09280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1406546.1"
                     /translation="SSKEADMNYVTAGQASIDRVVKPDGTCLGTNLGGPGVFAYTGVR
                     IWDDSVKMILNVAEDFYEYYGDWLRSNHVDQSALETVYDHTHSSDLIYEEDGSYSQSA
                     RSVEELVDRAAEYGWTNVRPAQIEAHTRNAQAFYLFVEPLYLHFWKQLREIRNRNDFE
                     LMWEIGIAKGGRYDRGHILNILELVRPEMASMNHNEARDFFGEEDREEIFHRIHTWKI
                     PMFFYRAGSAGSYLLHRGKSWFVPSIDLEGAPYVDATGCGNSSTAAAMYAWRKTRNPV
                     MTAIIANVTGGINVSCAGIIKSFDSALRETAFRTAAEVYDQYTRDHPDYCRDGYPHSA
                     DQVLALLREKR"
BASE COUNT          584 a          837 c          913 g          524 t
ORIGIN      
        1 tgggcagcgc gatgtacagc gcaaaggcgg aaccgatccc cgtgaggagg ccggagagat
       61 aaaaccaccc gatggagtga tagctgccca tgagggccat gcccacaaac tggagcagga
      121 gggcgcaggc gaccaccttg ccgatgggcc gggtggtgag cacgcggctg gcggtggtgt
      181 aaagcagcgc catggaggcc gcctggatgc tgatgaacat ggtgaaagag ctgacagagc
      241 agccgagctc ctccaccacg ggggtgacaa aattgccgca ggcgctggtg atgaccccca
      301 tccccgcaaa agccatcagc atacaacagc ccaggatgac ccaggcgtaa tgaaacgggc
      361 cggtcttttt catacgaaga agtctccttt tgacgaaatt aataaagcgc gcagaagagt
      421 acctgcgcgc aggcggatgc cgacgtgaaa aacgccggct ttgctcctgc acgcaggtgt
      481 cctttcgtca acacgggtca taaaatgaga ggccgggagg ctccgaggcg cggcgtggcg
      541 gctggcgggg agagaccgcc gtcacgtaac ctctcgacgg cgcgataaaa aggcgggaga
      601 gctgcagggg gacattcccg tcccgcgagc agacaaggct ttccaggcag atcaccatcg
      661 tgtcctgggg gatctccaac tgctgggaat gcttgtggga catggcgcgg taacggacgc
      721 attgctccac gagaaagccg tccagcacac agcggatcgc cggggcctcc agcgtcagat
      781 ccagcggctt tttgcaggcg ctgtccggcc gccaggacat ctcacaggcg agagggcgtc
      841 caccgtggcg gatgatgcgg cgaaagtatt tcacctgatc cgccggcgcg agcccgagaa
      901 ggtctgagac agcgttggcg gggcaacggt aacgggacat ccactcaaag ctgtaatcga
      961 ccggatgcca gatcgcggcc gccgcggggc cgcacaggat cgcctgcgcc tcaaagggca
     1021 ggcccgcttc cccctcgggg taagcggcga gacagtatag atattcgggt tctctgcagg
     1081 ccatagacgg cgtcatcctc cctcttttct gccggcctct agagtgtaga cgatggatcg
     1141 cagatcgctg taattgcggt agtattcgat caccctttgg cggttctgcg accagacgat
     1201 gccgctggag accgccacgt tcccccgcgc gcccagcagg gcggcgtcct cggcggccag
     1261 cgctgagata cagatgatcg agcggctcac aaagggctcc agatccagct cttgaaactt
     1321 ttttcggatc gattcccgat tgaagtccgt gtggagaaaa tcagggaaca ggtccagcgg
     1381 cgtataggag tattccaggc acataaaggc gccctctctg cggcgcagcc ttttgagctc
     1441 caggacaggc gcgtcggcgg aaagctccat cacctgccgg agatagcccg agggcaatat
     1501 tttgcgctgg ctcagggtct gggtggagag aaccacaaaa ttggggtatt gatgccgccg
     1561 cagattggag gcggcggagg tggtgagtac ctcaaagggc caggcgccca catattgccc
     1621 ggagcgggga cgggtgtaga gcgtgccctc cctgaccaaa atggccacgg cgtcgtggat
     1681 gcactggcgg gatacgccga acagcgcgca catctcccgc tgagagggca gcttgctgcc
     1741 cggtctcaga ccctgttccc ggatgtagcc gcggatcttt tcgcaggtga gggcccgctt
     1801 gttggcccga taggtgtttt tcatcgcttt tcccgaagca gggcgagcac ctggtcggcg
     1861 ctgtggggat acccgtcccg gcagtagtcc ggatggtccc gcgtgtactg gtcatagacc
     1921 tcggccgcgg tgcggaaagc cgtctcccgc agggcgctgt caaagctttt gatgatgccc
     1981 gcgcaggaca cgttgatgcc gccggtgaca ttggcgatga tcgccgtcat caccgggttg
     2041 cgcgtcttgc gccaggcgta catcgcggcg gcggtggaag aattgccgca gcccgtcgcg
     2101 tccacatagg gcgcgccctc caggtcgatg ctggggacga accagctttt gccccgatgg
     2161 agcagatagc tgccggcgct gccggcgcgg taaaagaaca tgggaatctt ccaggtatgg
     2221 atgcggtgaa agatctcctc ccggtcctcc tcgccgaaaa agtcccgcgc ctcgttgtga
     2281 ttcatactcg ccatttccgg ccgcaccagc tccaggatgt tgaggatatg cccgcggtcg
     2341 tagcggcctc ccttggcgat gccgatctcc cacatcagct cgaaatcatt ccggttgcgg
     2401 atctcccgca gctgtttcca gaagtggaga tagagaggct ccacaaaaag ataaaaggcc
     2461 tgtgcgtttc tggtgtgggc ctcgatctgt gcggggcgca cattggtcca gccatactcg
     2521 gcggcccggt cgaccagctc ctccacagac ctggcagatt gagaatagga gccatcctcc
     2581 tcgtagatga gatcagagga gtgggtgtgg tcgtagaccg tctcaagggc gctctgatcc
     2641 acatgattgc tccgcagcca atccccataa tactcataaa aatcctcggc gacattgagg
     2701 atcatcttta cgctgtcgtc ccagatgcgg accccggtgt aggcaaagac gccgggcccg
     2761 cccagattgg tgccaagaca ggtgccgtcg ggtttgacca cccggtcgat cgaagcctgt
     2821 ccggcagtga catagttcat gtcggcctcc tttgaact
//
LOCUS       DBMDYA010000374         2841 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7786_length_2841_cov_1.405474, whole genome shotgun sequence.
ACCESSION   DBMDYA010000374 DBMDYA010000000
VERSION     DBMDYA010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_2841_cov_1.405474"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2346
                     /locus_tag="ACZPFK_09285"
     CDS             <1..2346
                     /locus_tag="ACZPFK_09285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1406547.1"
                     /translation="SSKAHKLESLETCGAEVQKGNAPIERKLQRLFRNGEVTRLIKRC
                     NDFGAGGVSVAIGELADGLDIDLDKVPKKYDGLDGTELAISESQERMAVVLAPGDVKA
                     FIRAANEENLEATAVALVTESPRLVMRWQGETIVDVSRAFLNSNGAEKHADAKVGAQR
                     LRQPLPEGGSFGEKLEKLVGDLNVCSKKGLSERFDSTIGAATVLMPFGGRYQLTPAQA
                     MAAKLPVLSGDTDACSGMAWGFNPVVAKQSQYHGAYLAVVESVSKLVASGFSHEKAYL
                     TFQEYFERLGADPARWGKPLASLLGALDAQMDLDIASIGGKDSMSGSFEALDVPPTLV
                     SFAVAMGEAHRVASPEFKLPGSKVVYLAPETKDGFLPDPDSLKAVWSAVEELIAAEEV
                     LSAWTPGCGGVAEGLFKMCLGNRVGFKLDGSVDPEALFGWGYGGFILELAEELDIGLP
                     LGETTGSYTFEAAGETLSLEKLQAVWEDKLEPVLPYHTPAGEKTVEKVTFEVAPEQRR
                     APKFGCARPKVLIPVFPGTNCEYDTARAVKNAGGEPQILVVNNLSPARVAESADALRR
                     AIDGSQIVVLPGGFSGGDEPDGSGKFITAFFRNPAITDAVHRLLEQRDGLMLGICNGF
                     QALIKLGLVPFGRIVDTDASCPTLTYNTIGRHQSMLVRTRIASNKSPWLLHCRPDEIY
                     TVAVSHGEGRFVADKATLARLIENGQIATQYVGLSGEPTMDLRCNPNGSALAVEGITS
                     PDGRVFGKMGHSERSGEDLYRNVPGNKFQPIFQGGMDYYKL"
BASE COUNT          506 a          901 c          973 g          461 t
ORIGIN      
        1 tcctccaagg cgcacaagct ggagagcctc gagacctgcg gcgcggaggt gcaaaagggc
       61 aacgccccca tcgagcgcaa gctgcagcgg ctgttccgca acggagaggt cacccgtctc
      121 atcaagcgct gcaatgattt cggcgccggc ggcgtgtcgg tggccatcgg cgagctggcc
      181 gacgggctgg acatcgatct ggacaaggtg ccaaaaaaat atgacgggct ggacggcacc
      241 gagctggcca tcagcgagag tcaggagcgt atggctgtgg tgctggcccc cggggatgtg
      301 aaagcgttta tccgggccgc caacgaggag aacctggagg cgaccgccgt ggccctcgtc
      361 accgagagcc cgcgccttgt aatgcgctgg cagggcgaga ccattgtgga tgtgagccgc
      421 gcctttttga actccaacgg cgcggagaag cacgccgacg ccaaggtggg cgcccagcgg
      481 ctgcgccaac cgctgcccga gggcggcagc ttcggtgaaa agctggaaaa attggtggga
      541 gacctgaacg tctgcagcaa aaaaggcctg tccgagcggt ttgattccac catcggcgcg
      601 gccacggtgc tgatgccctt tggcggccgt tatcagctga ccccggccca ggcgatggcc
      661 gccaagctgc cggtgctgtc gggggatacc gacgcctgca gcggcatggc ctggggcttt
      721 aacccggtgg tcgccaagca gagccagtat cacggggctt atctggcggt ggtggagagc
      781 gtctccaagc tggtggcctc cggcttctca cacgaaaagg cctatctcac gtttcaggaa
      841 tatttcgagc ggctgggcgc cgacccggcc cgctggggca agcccctggc gagccttttg
      901 ggcgcgctgg atgcccagat ggacctggac atcgcctcca tcggcggcaa ggacagcatg
      961 tccggcagct ttgaggcgct ggacgtgccg cccacactgg tctcgtttgc ggtggccatg
     1021 ggcgaggccc accgggtggc ctcgccggag ttcaagctgc ccggcagcaa ggtggtctac
     1081 ctcgctcccg agacaaagga cggctttttg cccgacccgg acagcctgaa agcggtctgg
     1141 agcgcggtgg aggagctgat cgccgccgag gaggtgctct ccgcctggac gccgggctgc
     1201 ggcggcgtcg ccgagggcct gttcaagatg tgccttggca accgcgtcgg ctttaagctg
     1261 gacgggtcgg tggacccgga ggcgctgttc ggctggggct acggcggctt tatcctggag
     1321 ctggcggagg aattggacat cggcctgccg ctgggtgaga ccaccggcag ctataccttt
     1381 gaggccgccg gcgagaccct ttcgctggag aagctgcagg cggtgtggga ggacaagctg
     1441 gagccggtgc tgccctatca cacccccgcc ggggaaaaga cggtggaaaa ggtcactttt
     1501 gaggtggccc ccgagcagag gcgggcgccc aagttcggct gcgccaggcc aaaggtgctg
     1561 atcccggtct ttcccgggac gaactgtgag tacgacaccg cgcgcgcggt gaagaacgcg
     1621 ggcggcgagc cgcagatctt ggtggtcaac aacctctcgc cggcgcgggt ggccgagagc
     1681 gccgacgccc tgcgccgcgc catcgacggc agccagatcg tggtgctgcc gggcggcttt
     1741 tccggtgggg acgagccgga cggcagcggc aagttcatca ccgctttctt ccgcaacccc
     1801 gccatcaccg acgcggtgca ccgcctgctg gagcagcggg acggcctgat gctgggcatc
     1861 tgcaacggct ttcaggccct gatcaagctg gggctggtac cctttggcag gatcgtggac
     1921 accgacgcct cctgccccac cttgacctac aacaccatcg gccgtcacca gagcatgctg
     1981 gtgcgcaccc gcatcgcctc caacaaatcc ccctggctgc tgcactgccg cccggatgag
     2041 atctacaccg tggccgtcag ccacggcgag ggccggtttg tggcggacaa ggccacgctg
     2101 gcgcgtctga ttgaaaacgg ccagatcgcc acccagtacg tgggcctctc cggcgagccc
     2161 accatggacc tgcgctgcaa ccccaacggc agcgccctgg cggtggaggg catcaccagc
     2221 ccggacggca gggtcttcgg caagatgggc cactcggagc gcagcggcga ggatctgtac
     2281 cgcaacgtgc ccggcaacaa gttccagccc atcttccagg gaggcatgga ttattacaaa
     2341 ctgtaatgaa ttattgcaat agctctaatt gtaaaaaaca aagcgagaaa aacagagaat
     2401 gcgctggccc gcgcaggctt ccaggctcgc cggcctgagg ccagaggtcc gctggcccgg
     2461 aggagcacaa cggaaatccg cgcccgtgct tgcgggacgc ggaggcgggc ggggcgcagc
     2521 cgccctgtga gaaagggccc ctccacccgg ggcggccttt gccgccgggc ttccgtcgcc
     2581 cgcgcgggca ataccgagac ggcgcgaccg cgcgccgggc ctcctccccc tgcggggtgg
     2641 gggggcgctg agagcggtta cttcccttgg gggcagagcg ggccgccgct gaaaggccgc
     2701 tcgcaccgtg ccgtgcggcc gcgtttttta tctttttctc ttatcaaaac cggaggtttt
     2761 caccatgtca cacgaccgct atatcagccc tttccagtcg cgctatgcca gcgaggagat
     2821 gcagtacatc ttctcggagg a
//
LOCUS       DBMDYA010000375         2834 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7803_length_2834_cov_1.176729, whole genome shotgun sequence.
ACCESSION   DBMDYA010000375 DBMDYA010000000
VERSION     DBMDYA010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7803_length_2834_cov_1.176729"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            10..177
                     /locus_tag="ACZPFK_09290"
     CDS             10..177
                     /locus_tag="ACZPFK_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406548.1"
                     /translation="MAGQSSVIPADQCIDAMYRVGKELPMELRETALGGIASQPAGKA
                     YAKQLREIGRI"
     gene            complement(418..2094)
                     /locus_tag="ACZPFK_09295"
     CDS             complement(418..2094)
                     /locus_tag="ACZPFK_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406549.1"
                     /translation="MKVKKVSNVAAKVADKLGAWMLLIVIAVIMVFASDKFLTVDNLL
                     NVFRQIAVVCIGAIGVSLVILGGGFDLSTGMIATFAGCNTAIFMKNFGWNMFVAMAVA
                     VLIGVVIGTANGLLITYLKIPPFIGTLGMQYIVNGIIFVTTKSVPITGLPEAFLTLGR
                     GYVADIIPVPVIIMLVFVLLGLFLTKPFLSLFTSSPVILSLGADYLFIVLVGCVGQQV
                     QIAIEKMLQATGNMVWPMLMQALGCVVNIILDPIMIFGLFGFPEMGVRGAALATIIGQ
                     LASMALAFFALFKKEHEVRLDLRRFKFRPSVIGDIYNVGVPTILMNSLGSVLVMGLNS
                     LLMRFSEVAVSVFGIYYKLQTFVFMPVSGMAQGAMPIMGYNYGARSRGRVVACFRDSL
                     IVSAAILAAGNLLFVLLPAPLLALFGAGPEMMAIGVPALRILGCSYLPAALGCCIPTL
                     FQAVGRGRSSLVIFLLRQFAITLPLAWLLAGPMGLAGIWCSFIVAEVAAAAAAGLMLG
                     QLFRRDPVLCRRDAASAGQAPAEKASGHAAPPAPAPESAGTDGEAPAPQA"
     gene            complement(2091..>2834)
                     /locus_tag="ACZPFK_09300"
     CDS             complement(2091..>2834)
                     /locus_tag="ACZPFK_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1406550.1"
                     /translation="GEVTLEVRNLTDGGKHVSDVSFAARRGEVLGFYGLVGAGRTETM
                     RMIFGVDKMLGGQVLLHGSEITLRNPRDAIRHGLGFITENRRDEGVFLRSSVRVNTVM
                     VALDKILSYGFINYKKEAEVSQHYVDKLRTATPTINQKAMFLSGGNQQKVVLSKWLFS
                     DSEIIIFDEPTRGIDVGARREIYQIINDLVADGKTVVVVSSDMEEIMGVSDRILVMYE
                     GKVAGEVRKEDFSQSLITEYAVGERTQPV"
BASE COUNT          544 a          884 c          939 g          467 t
ORIGIN      
        1 gatctggcgc tggcggggca gagctcggtc atcccggctg accagtgcat cgacgccatg
       61 taccgggtgg gcaaagagct gcccatggag ctgcgggaga ccgccctggg cggcatcgcc
      121 tcccagcccg cgggcaaggc gtacgcgaag cagctgcggg agatcggcag gatctgattg
      181 gcccttgtgc gggcgctgcg cggggcaaag cgatggggcc cgcccccacg gcctttaagc
      241 ggcgcgggcg cgcaccggga catttttggc gggcctttgc ccgcccgccg ctgccgtgct
      301 tgttgctctt gccgcgcggg ctgccgacgg agaagattag cttgatggat ttgagaaaaa
      361 agcgccccgt atcagcgata cggggcgctt ttggcgtgtc tgtagatttt taggggctca
      421 ggcctgcggg gcaggggctt cgccgtccgt ccccgcgctt tcgggggcgg gcgcgggagg
      481 ggcggcgtgg ccggaggcct tttcggccgg ggcctgcccc gcgctcgcgg cgtcgcggcg
      541 gcagagcacc gggtcgcggc ggaagagctg tcccagcatc agcccggccg ccgcggctgc
      601 ggcgacctcg gcgacgatga aagagcacca gatgcccgcc agccccatgg ggccggccag
      661 cagccaggcc agcggcaggg tgatggcaaa ctgccgcagc agaaagatca ccaggctgga
      721 gcggccgcgc cccaccgcct ggaacagggt ggggatgcag cagcccaggg ccgcgggcag
      781 atagctgcac ccgaggatgc gcagggcggg cacgccgatg gccatcatct cgggcccggc
      841 gccgaacagc gccagcagcg gggcgggcag caggacaaac agcagattgc ccgccgccag
      901 aatggcggct gagacgatca ggctgtcccg aaagcaggcc accacccgcc cgcggctgcg
      961 ggcgccgtaa ttgtagccca tgatcggcat ggcgccctgc gccataccgc tgaccggcat
     1021 aaagacaaag gtctgcagct tgtaatagat gccgaacacc gagaccgcca cctcggaaaa
     1081 gcgcatcagc aggctgttga ggcccatcac cagcacactg cccaggctgt tcatcaaaat
     1141 ggtgggcacc cccacgttgt agatgtcgcc gatcaccgag ggacggaatt tgaaccggcg
     1201 caggtcgagc cgcacctcgt gctctttttt gaacagtgcg aaaaaggcga gggccatcga
     1261 ggccagctgg ccgatgatgg tggcgagggc cgcgccccgc acccccatct cgggaaagcc
     1321 gaacagcccg aagatcatga tcgggtcgag gatgatgttg accacacagc ccagcgcctg
     1381 catcagcatg ggccagacca tgttgccggt ggcctgcagc atcttttcaa tggcgatctg
     1441 cacctgctgg cccacgcagc ccaccagcac gatgaacaga tagtcggccc ccaggctgag
     1501 gatgaccggc gagctggtga aaagggagag aaacggcttt gtgagaaaca ggcccagcag
     1561 cacgaagacc agcatgatga tgaccggcac cgggatgatg tccgccacat agccgcggcc
     1621 gagggtcagg aacgcctcgg gcaggccggt gatgggcacg ctcttggtgg tgacgaagat
     1681 gatgccgttg acgatgtact gcatgcccag ggtaccgatg aagggcggga tcttgagata
     1741 ggtgatgagc aggccgttgg ccgtgccgat gaccacgccg atgagcacgg ccaccgccat
     1801 ggcgacgaac atgttccagc cgaaattctt cataaagatg gcggtgttgc agccggcaaa
     1861 ggtggcgatc ataccggtac tgaggtcgaa gccgccgccc aggatgacca gcgagacgcc
     1921 gatggcgccg atgcagacca ctgcgatctg gcgaaagacg ttgagcaggt tgtcaacagt
     1981 gaggaacttg tcgctggcaa agaccatgat caccgcgatg acgatgagca gcatccatgc
     2041 gcccagcttg tcggcgacct ttgccgcgac gttggaaact ttttttactt tcatacgggt
     2101 tgggtccttt ctcctacagc gtattctgtg atcaggctct gcgaaaaatc ttctttgcgc
     2161 acctcgcccg ccaccttgcc ctcgtacatg accaggatgc ggtcgctgac gcccatgatc
     2221 tcctccatat ccgaggagac gacgaccacg gtttttccgt cggccaccag gtcgttgatg
     2281 atctggtaga tctcacggcg ggcgcccacg tcgataccgc gggtcggctc gtcgaaaata
     2341 atgatctcag agtcggagaa cagccacttg gacaaaacga ccttctgctg gttgccgccg
     2401 ctgaggaaca tggccttttg attgatggtg ggggtggcgg tgcgcagctt gtcgacatag
     2461 tgctgggaca cctccgcctc ctttttataa ttgatgaaac cgtagctgag gatcttgtcc
     2521 agggccacca tgacggtgtt cacccgcacc gacgagcgca ggaacacccc ctcgtcgcgc
     2581 cggttctcgg tgatgaagcc caggccgtgg cggatggcgt cgcgcgggtt gcgcagcgtg
     2641 atctcgctgc cgtgcagcag cacctggccg cccagcatct tgtccacgcc gaagatcatg
     2701 cgcatcgtct cggtgcgccc ggcgcccacc aggccgtaaa agcccagcac ctcgccgcgc
     2761 cgcgccgcaa aggagacgtc gctcacgtgc ttgccgccgt cggtgaggtt gcgcacctcc
     2821 agcgtcacct cgcc
//
LOCUS       DBMDYA010000376         2827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7822_length_2827_cov_1.385913, whole genome shotgun sequence.
ACCESSION   DBMDYA010000376 DBMDYA010000000
VERSION     DBMDYA010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7822_length_2827_cov_1.385913"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            644..1321
                     /locus_tag="ACZPFK_09305"
     CDS             644..1321
                     /locus_tag="ACZPFK_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1406551.1"
                     /translation="MPRNKHPEETVDRILNVALALFLEKGYEATTIQDIIDHLGGLSK
                     GAIYHHFRSKEEIMIAVGDRLGQENARMLGAVRDDPTLTGREKLVAIFRASLRNPNQE
                     AMFRVALAIQKNPRFLALQLEDTFELVAPKYIQPILEQGLRDGTIAAADPQGLAEVMM
                     LLANVWLNPMILISTPEKMAQRCRLYNDLMRPQLGFDLLDEEMLQKYIEYCSGYTRTM
                     EEKGGRD"
     gene            2260..>2827
                     /locus_tag="ACZPFK_09310"
     CDS             2260..>2827
                     /locus_tag="ACZPFK_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406552.1"
                     /translation="MKKTEAPGAAIFRRDFTLVVVGQIISLFGNAILRFALPLYLLRA
                     TGSSALFGMVTAASFLPMIVLSLLGGVLADRVNKRNIMVALDFSTAALIAVFYLVLGR
                     LPIVPLFTVMLMLLYGVSGAYQPAVQASIPALVEGGGLMSANAVINAVNSLAGLLGPV
                     LGGVLYGLWGIGPILLVSILCFFASAVME"
BASE COUNT          476 a          871 c         1005 g          475 t
ORIGIN      
        1 ccgcttctca acaagacaac ggcgcacaat tggccctctg ccgcgccccc gccccaaaaa
       61 ataaacgacg cccaaggctc ccttgtgtaa agggagctgt cagcgaagct gactgaggga
      121 ttgccggcag gcggcctgga tatatccttg cactcccccg cgagagccgc cgccgatctc
      181 cctgctgaaa atccgcagca atccccgcaa cccgtagctc tctaaaaagg gggaaaactc
      241 gccccccaac aagacgacgg ccccaaacca ctggcgggag ggccggggcg cgggcggcgg
      301 aacagccggg aaaccggccc agccgtgaga cgtcccccgg ccttgcggaa agcgatgccg
      361 ccccggatgg ggcgccgccg cggcgcgggc ccgcggacaa aacgggggcg ttttgggggc
      421 gggacaggcg aaagtcgggg gcgcgacctt gccgggctcc ggctgcgggt ggacgccgcg
      481 cggggccatg tgggcaaaac ggctttttct ccgccttggg gcgcacccgg agggaagacg
      541 atggtcgaga gagaggcgtc gggccgtttc cccttgacaa aggtaaaaaa ttgtgttaaa
      601 aatagataca taccgtcggt atgtaattga gaggaggcgc gccatgccgc gcaacaagca
      661 cccggaggag acggtggacc gcatcttgaa cgtggctctg gccctgtttt tggaaaaggg
      721 ctatgaggcc accaccatcc aggacatcat cgaccacctg ggcggcctct caaaaggggc
      781 gatctaccac catttccgct ccaaagagga gatcatgatc gcggtggggg accggctggg
      841 gcaggagaac gcccgcatgc tgggcgccgt gcgggacgat cccactctga ccgggcgcga
      901 aaagctggtg gcgatcttcc gcgcctcgct gcgcaacccc aatcaggagg cgatgttccg
      961 ggtggcgctg gccatccaaa agaatccgcg ttttctggcc ctgcagctgg aggacacctt
     1021 tgagctggtg gcgccgaagt acatccagcc cattttggag cagggcctgc gggacggcac
     1081 catcgcggcc gccgaccccc aggggctggc ggaggtgatg atgctgctgg ccaacgtctg
     1141 gctgaacccg atgatcctca tcagcacgcc ggagaagatg gcgcagcgct gccggcttta
     1201 caacgacctg atgcggccgc agctgggctt tgacctgctg gacgaagaga tgctgcagaa
     1261 gtacatcgag tattgcagcg gctacacccg cacgatggag gaaaagggcg gccgggattg
     1321 agagccccgg gcgcgcctgc cccggcggcc ggaaaggggc gggcggaggc gtttgcggcc
     1381 cctgcctggg gcggcggaaa agagcggggc tgaagcgccc ggcgcagccc cgcgcgggag
     1441 cggccgccgt ctgttcccgt cgggagcaaa gcccgtcggc cgcgtcgccg cccgcgggca
     1501 ggggaggatg cggccgcgcc gccgccttgt tgtcggggct ttttcggcag cagccgaggg
     1561 gcgcgggctt gctttgccgg gcaagatcga atgcccccgc cctcaccgcg cttggggtaa
     1621 gccccactct tttgcgcgcc gaagcgcagg gatgtgaaag ccctgcgcgc tacgggcggc
     1681 actgcgcctg cggcggctgc cctcgcgcct gtgcggcgga gctccggctg ctgggcgcgc
     1741 agctggtgac gggaaagcgg cggccacgtt gtcggcgcgg tcggtcggcg tttgccccgc
     1801 atgggggaca gcttttggct tcgctctccg ctggagggcg gcagccggag ggaaaaaagc
     1861 gatggctgtg gggtctgcgc gggttttctc gtttgcccgg gcgtccggcg acagtctctg
     1921 gctgcggccg ccgctggaaa gacggcaaaa cagggagcta aaagcccgcc cggaagccgg
     1981 agaaaaaacg cggggaaacc ggcggcgggg cgaagagcgg cttggcgctt tcggcccccg
     2041 gctttggggc ctgggacggg gccgggggac ggcgcggttt ttgcagagcc ggggacgcgc
     2101 gtcgggggcc ggaagcgcgc gccgtttcgc gggggaaaag cccggctttg cgccgcgacg
     2161 ggtttgagct ctggtctgtt ttcagaggat tatttttttt gcatcaatac ataccaaccg
     2221 ttggtattaa aaaaatttaa ggagactctc atgcaatcta tgaaaaaaac tgaggccccc
     2281 ggggccgcga ttttccggcg cgacttcacg ctggtggtcg tcgggcagat catctcgctg
     2341 ttcggcaacg ccatcctgcg ctttgccctg cccctttacc tgctgcgagc caccggctcg
     2401 tcggcgctgt tcggcatggt gacggccgcc tcgtttctgc ccatgatcgt gctctcgctg
     2461 ctgggcgggg tgctggccga ccgggtcaac aagcgcaaca tcatggtggc gctggatttt
     2521 tccaccgcgg cgctgatcgc ggtgttctat ctggtactgg gcaggctgcc cattgtgccg
     2581 ctgttcacgg tgatgctgat gctgctgtac ggcgtctccg gcgcctacca gccggcggtg
     2641 caggccagca tcccggcgct ggtggagggc ggcggcctga tgtccgccaa cgcggtcatc
     2701 aacgccgtca actcgctggc ggggctgttg ggaccggtgc tgggcggggt gctgtacggg
     2761 ctgtggggca tcggccccat cctgctggtg agcatcctct gctttttcgc ctccgcggtc
     2821 atggagg
//
LOCUS       DBMDYA010000377         2824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7829_length_2824_cov_1.568491, whole genome shotgun sequence.
ACCESSION   DBMDYA010000377 DBMDYA010000000
VERSION     DBMDYA010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_2824_cov_1.568491"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="ACZPFK_09315"
     CDS             <1..336
                     /locus_tag="ACZPFK_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1406553.1"
                     /translation="IGLAHQDDLHAAMEEIADVIGCGLFAHESIPAAFGYIAAARGRV
                     MDCLYMAINAGDDTDTVACMTGFITGAYCSSRDLSPRYLALIDEVNHFDLKEMACQID
                     AIAGNTPEA"
     gene            383..1405
                     /locus_tag="ACZPFK_09320"
     CDS             383..1405
                     /locus_tag="ACZPFK_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1406554.1"
                     /translation="MKQDKVLGALLAAAIGDAMGAPLDSLPVYLIKQNCGGGDFVRDY
                     VAPPQGALAPELKKGMVAGSFSSAFASAEAFIKHGGISGEAVAEGLLEWKNGRYKFYF
                     DCCADPATRYCLQRMEGTAASEDQDKFTGFQKTINSGAAARAWVAGLFHPGDPDGAVR
                     DAIAMGLPTHDNVIALSGACAVAAGTACAMMEKADTGRIVEAGLYGARTGYRKALEVA
                     GDAAGASVEKRIRLAVQIGLRCANDFERCVNEMTDLVGTGSPANEAVPAAFGFLVSAG
                     GEVMETLYRSINAGNASSVVATMAGAMAGAFRGCAGLPPQHLEALSAINGMDLAQAAR
                     AISALI"
     gene            1425..2456
                     /locus_tag="ACZPFK_09325"
     CDS             1425..2456
                     /locus_tag="ACZPFK_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1406555.1"
                     /translation="MQNELYAKIHGALLAAAVGDSMGTVTEGFAPEFLRSRYGGYVRD
                     LLQPTDDGLTVNARAGMVSDDFSVAYYTAQVLLEHQSRITTALAVEGLKRWWQHPEYT
                     CYCGPSTRRGILKLLGEEAGDTASSRLDPTFATNGGGMKAGMMGLFNPGDPAGAVTDA
                     ITMCLPTHGNPISLSAACAVAAAAARAFCPSTGYLDLIAAGMGGISQCLAQSAGRTAE
                     IAGCSVARRLELAVELGLRCQDDFEQALFRIGDTVGAGLYAYEAVPAAFGILAACKGR
                     LMDSLIMAVNMGSDADSTACMLGYVLGAWNGDAELPPHFSALIDSQNGFDLEAMARGI
                     CDLAKEGIC"
     gene            2453..>2824
                     /locus_tag="ACZPFK_09330"
     CDS             2453..>2824
                     /locus_tag="ACZPFK_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406556.1"
                     /translation="MKAFDLPARMKRTVRAVAAADAFALPMRGLGPDQIRRDLGGGDL
                     VRRLCKAPGAAGDSFEPGGVSDVFASSYLLLRGFLRDGGRVTLAGAGQALLRLKTESI
                     FRPYLDRFLDPVTRESLDRLDG"
BASE COUNT          452 a          920 c          965 g          487 t
ORIGIN      
        1 atcggcttgg cgcatcagga cgacctgcac gccgccatgg aggaaatcgc cgacgtgatc
       61 ggctgcggcc tgtttgcgca tgaatccatc cccgccgcgt tcggctatat cgccgccgcg
      121 cggggccggg tgatggactg cctttatatg gccatcaacg cgggcgatga cacggatacc
      181 gtggcctgta tgacgggctt catcaccggt gcgtactgct cgtcgaggga cctctctccc
      241 agatacctgg ccctgatcga cgaggtcaac cacttcgacc tgaaagagat ggcatgccag
      301 atcgacgcca tcgcgggaaa tacgccggag gcctgacccg ggccctgcgg cccggcgcat
      361 cttggaaagg acggataaca aaatgaaaca ggacaaagtg ctcggcgccc tgctcgcggc
      421 ggcgatcgga gacgcgatgg gcgcccccct cgactccctg ccggtctatc tgatcaagca
      481 gaattgcggg ggcggagatt ttgtgcggga ttacgtggcg ccgccccagg gcgccctcgc
      541 ccctgagctg aaaaagggga tggtcgccgg ttctttcagc tccgctttcg cctctgcgga
      601 ggctttcatc aaacacggcg gtatctcggg cgaggcggtg gcggagggcc tgctggagtg
      661 gaaaaacggg cgctacaaat tttacttcga ctgctgtgcg gaccccgcca cccgctactg
      721 cctgcaaagg atggagggga cagccgcctc ggaggatcag gacaagttca ccggctttca
      781 aaagaccatc aacagcggtg cggcggcccg ggcctgggtc gcgggcctgt tccaccctgg
      841 cgacccggat ggggccgtgc gggatgccat cgcgatgggc ctgcccaccc atgacaacgt
      901 catcgccctc tccggcgcct gcgccgtcgc ggccggtacg gcctgcgcca tgatggagaa
      961 agcggacacc ggccgcatcg tggaggcggg cctgtacggc gcgcggaccg gctaccgcaa
     1021 ggcgcttgag gtggcgggcg acgccgccgg agccagcgtg gaaaagcgca tcaggctcgc
     1081 cgttcagatc gggctgcgct gcgcgaacga ttttgagcgc tgcgtcaacg agatgaccga
     1141 cctggtgggc accggctccc ctgccaacga ggccgtgccc gcggctttcg gttttctggt
     1201 atcggccggc ggggaggtca tggagacact ctatcgctcg atcaacgcgg gcaacgccag
     1261 cagcgtggtt gccacaatgg cgggcgcgat ggcgggggcc tttcgcggct gtgccggcct
     1321 gccgccgcag caccttgaag ccctgtccgc gatcaacggg atggacctgg cacaggcggc
     1381 ccgcgcaatc tcggcgctca tctagcgccg ttcaggaggg gatcatgcag aacgaactgt
     1441 acgcaaaaat ccacggggcg ctgctggcgg ccgcggtggg cgattcgatg ggcacggtga
     1501 ccgaggggtt cgcccccgag tttctccgca gccggtacgg cggctatgtc cgcgatctct
     1561 tgcagcccac cgacgacggg ctgaccgtca acgcccgcgc aggcatggtc agcgacgatt
     1621 tttccgtggc ctattatacc gctcaggtgc tgctggagca tcaaagccgc atcacgaccg
     1681 ccctggccgt ggagggcctc aagcgctggt ggcagcaccc ggaatacacc tgctactgcg
     1741 gccccagcac ccggcggggc atcctgaagc ttttggggga ggaggcgggc gatacggcct
     1801 cctcccgcct cgatcccacc ttcgccacca acggcggcgg catgaaagcg ggcatgatgg
     1861 gactgttcaa cccgggcgac ccggcagggg ctgtcaccga cgccatcacg atgtgcctgc
     1921 ccacccatgg caaccccatc tccctctccg cggcctgcgc ggttgcggct gcggccgccc
     1981 gtgccttttg ccccagcacg ggctacctgg acctgatcgc cgcaggcatg ggcggcatct
     2041 cgcaatgcct cgcacagagc gcggggcgca cggcggagat cgccggctgc agcgttgccc
     2101 gccgtctgga gttggcggtg gagctgggcc tgcgctgtca ggatgatttt gagcaggcgc
     2161 tgttccgcat cggcgacacc gtgggggccg ggctgtacgc gtatgaggcg gttcccgccg
     2221 ctttcggcat cctcgccgcc tgcaagggcc gcctgatgga ctctctgatc atggcggtca
     2281 acatgggcag cgacgccgat tccactgctt gtatgctggg ctatgtgctg ggcgcctgga
     2341 atggggacgc agagctgccc ccgcacttct cggccctgat cgacagccag aacggctttg
     2401 acctggaggc catggctcgg ggaatctgtg atctggcaaa ggaggggatc tgttgaaagc
     2461 ttttgatttg cccgcccgca tgaagcgcac cgtgcgcgcc gttgccgccg cggacgcttt
     2521 cgccctgccc atgcggggcc tggggccgga ccagatccgc cgcgacctgg gcggcggcga
     2581 cctggtccgc cgcctgtgca aagcgcccgg cgctgcgggg gattcttttg agccgggcgg
     2641 ggtgagcgac gtttttgcct cgagctatct gcttttgcgc ggttttctgc gcgacggagg
     2701 acgcgtcacc ctcgccgggg ccgggcaggc tctgctgcgg ctgaaaaccg agagcatttt
     2761 tcgcccctac ctcgaccgct ttttggaccc ggtgacccgg gagagcctgg acaggctgga
     2821 cggc
//
LOCUS       DBMDYA010000378         2811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7858_length_2811_cov_1.387454, whole genome shotgun sequence.
ACCESSION   DBMDYA010000378 DBMDYA010000000
VERSION     DBMDYA010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7858_length_2811_cov_1.387454"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="ACZPFK_09335"
     CDS             <1..1050
                     /locus_tag="ACZPFK_09335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1406557.1"
                     /translation="PARRASPRCGAWATGWRWMGMHSREVWGLTAGMLGITAAAGLAG
                     LWLWGAGAAALLWLLGGLLTALAAAFTLWRYRQIGALCRYLAGVYAGGEAVDIRDQRP
                     GELGALKDDLYKITTVLRRQAADLRRQKAFLSETLENIAHQLKTPLAAIQLQTELWQS
                     PGTGQAQRARCAAEAARQVERMDWLVKSLLKLSRLEADAVPFERRELPAAEVIRRAAR
                     GLLPLLGRRQVELAVHCPQDALCRCDPAWTAEALTNLIKNCAEHTPPGGRVSVHFAAT
                     VLFDEFRVEDTGGGIPAAALPHIFERFWRGPGSPPGSAGIGLPLARAVAQGQNGALTA
                     ANTAEGACFTLRLFR"
     gene            1195..1875
                     /locus_tag="ACZPFK_09340"
     CDS             1195..1875
                     /locus_tag="ACZPFK_09340"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406558.1"
                     /translation="MSYLKVKDLSRTYGQGEGRVEALRGVSFEVQRGEFVAVMGPSGS
                     GKSTLMHLLGGVERPTAGEIELEGVDLLALPEEKRTLFRRRQIGLVYQFYNLVPLLTV
                     AENIALPLLLDGRAPAPGQLEGLAARLELSDKLDAYPHQLSGGQQQRAGIGRALITCP
                     ALLLADEPTGNLDSENTRQVMALLRGFHQSMGQTIIMITHDRELALQAQRILTLADGR
                     VVRDEVNR"
     gene            1872..>2811
                     /locus_tag="ACZPFK_09345"
     CDS             1872..>2811
                     /locus_tag="ACZPFK_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1406559.1"
                     /translation="MKAIWFLTARQLRRGGWRSGLALCSALLSVVLLSGTLLGGQALS
                     RALVKGDGASLLAPLVRAAAGVLILFLLLATGILIRSSFSASLAQRVKMLGQLAGVGA
                     TRRQLRASVYLEALLLAAVAIPVGLSATATGLWVAFELLNRSGAFVAQFGALRLSLSP
                     GLLGLCAGVALLDLLLAAGGPARRAFRIAPLDAVRRPMDAGSPHRARPWRQGCGAPAQ
                     LARRSLTRAGARFRPLAASIAACVAAVLVCAGFIDGVRQAYAANVPSYRYRLYLWGEA
                     GARPDAALEEIAGWDDVLVAEETEYYSAWGQTLTLIL"
BASE COUNT          357 a          967 c         1056 g          431 t
ORIGIN      
        1 ccggcgcgcc gcgcatcgcc acggtgcggg gcatgggcta ccggctggag gtggatgggg
       61 atgcatagcc gcgaggtctg ggggctgacg gccgggatgc tcggcatcac cgccgccgcg
      121 gggctggcgg ggctgtggct ctggggtgcg ggggcggcgg ccctgctctg gctgctgggt
      181 ggtctgctca ccgccctggc ggccgcgttc accctttggc gctaccgcca gatcggggcc
      241 ctctgccgct atctggccgg ggtctatgcg ggcggcgagg ccgttgacat ccgcgaccag
      301 cgcccgggcg agctgggtgc cctcaaggac gatttataca agatcaccac cgtgctgcgg
      361 cggcaggccg ccgatctgcg gcggcaaaag gcctttctca gcgagacgct ggagaacatc
      421 gcgcaccagc tcaaaacacc gctggcggcc atccaactgc agaccgagct ctggcagagc
      481 cccggcacgg ggcaggcaca gcgggcgcgc tgtgcggcgg aggcggcccg ccaggtggag
      541 cggatggact ggctggtaaa gagcctgctc aagctcagcc gcctggaggc ggacgcggtg
      601 ccctttgagc gcagagagct gcccgcggcg gaagtgatcc ggcgggcggc ccgggggctg
      661 ctgccgctgc tggggcggcg gcaggtggag ctggcggtcc attgcccgca ggacgccctc
      721 tgtcgctgtg acccggcctg gaccgcagag gccctgacca atctcatcaa aaactgcgcc
      781 gagcacaccc cgccgggcgg ccgggtgagc gtgcactttg cggcgaccgt gctctttgac
      841 gagttccggg tcgaggacac cgggggcggc atcccggccg cggcgctgcc ccatatcttt
      901 gagcggttct ggcgcgggcc gggctctccg cccggctccg cgggcattgg cctgccgctg
      961 gcgcgcgccg tcgcccaggg gcagaacggc gccctcaccg ccgccaacac cgccgagggg
     1021 gcctgcttca cgctgcggct gttccggtga cggcatcgtc accccgcggt caccgccctg
     1081 tcagaaacgg cggttaaact gtggacaggc gtgggggatg cggctgtcgc cgccccaggc
     1141 ccggcaagcg ccgtcaaggc ggggccgagc gccccgcaag ggaggaaaaa acgaatgtcc
     1201 tatttaaagg tgaaagattt gtcgcgcacc tacggccagg gggagggccg ggtggaggcg
     1261 ctgcgcgggg tctcgtttga ggtgcagcgg ggggagtttg tcgcggtcat ggggccctcg
     1321 ggcagcggca aatccaccct gatgcatctg ctgggcgggg tcgagcggcc caccgccggg
     1381 gagatcgagc tggagggggt ggatctgctg gccctgccgg aggaaaagcg caccctgttc
     1441 cgccgcaggc agatcggcct ggtctatcag ttctacaacc tggtaccgct gctcacggtg
     1501 gcggagaata tcgccctgcc gctgctgctg gacggccgcg cccccgcgcc cggccagctg
     1561 gaggggctgg cggcgcggct ggagctcagc gataagctcg acgcttatcc tcaccagctc
     1621 tccggcgggc agcagcagcg ggccggcatc ggcagggccc tgatcacctg cccggcgctg
     1681 ctgctggcgg acgagcccac cggcaacctg gactcagaga acacccgtca ggtgatggcc
     1741 ctgctgcggg ggttccacca gtcgatgggc cagaccatta tcatgatcac ccacgaccgg
     1801 gagctggccc tgcaggccca gcgcatcctc acgctggcgg acggccgcgt ggtgcgggac
     1861 gaggtgaacc gatgaaagcg atctggtttc tcacggcccg ccagctgcgc aggggcggct
     1921 ggcgcagcgg tctcgccctc tgctcggccc tgctctcggt cgttctgctc tccggtaccc
     1981 tgctgggcgg gcaggcgctc tcccgcgcgc tggtcaaggg ggacggcgcc agcctgctgg
     2041 cgcccctggt gcgggcggcg gccggggtgc tgatcctctt tctgctgctg gccaccggca
     2101 tcctgatccg cagcagcttt tccgcctccc tggcgcagcg ggtgaagatg ctggggcagc
     2161 tggccggcgt gggggcaacg cggcgtcagc tgcgcgccag cgtctatctg gaggcgctgc
     2221 tgctggccgc cgtcgccata ccggtgggct tatcggcaac ggcgaccggc ctttgggtcg
     2281 cctttgagct gctcaaccgc agcggcgcct ttgtggcgca gttcggggcg ctgcggctct
     2341 ccctctcccc cggcctgctg gggctctgcg ccggggtggc cctgctcgac ctgctgctgg
     2401 ctgccggcgg ccccgcgcgg cgggcctttc gcatcgcgcc gctggacgcc gtgcgccgcc
     2461 cgatggatgc gggttcgccg caccgcgcgc ggccctggcg gcagggctgc ggcgcgcccg
     2521 cccagttggc ccgccgcagc ctgacccgcg ccggggcccg ctttcgcccc ctggcggcca
     2581 gcattgcagc ctgtgtggcc gcggtgctgg tctgcgccgg cttcatcgac ggggtgcggc
     2641 aggcctacgc cgccaacgtg cccagctatc gctaccgcct ctacctctgg ggggaagcgg
     2701 gcgcccgccc cgacgcggcg ctggaggaga tcgccggctg ggacgacgtg ctggtggccg
     2761 aggagaccga gtattacagc gcctgggggc agacgttgac cctaatcctg g
//
LOCUS       DBMDYA010000379         2806 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7872_length_2806_cov_1.399630, whole genome shotgun sequence.
ACCESSION   DBMDYA010000379 DBMDYA010000000
VERSION     DBMDYA010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_2806_cov_1.399630"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(122..334)
                     /locus_tag="ACZPFK_09350"
     CDS             complement(122..334)
                     /locus_tag="ACZPFK_09350"
                     /inference="COORDINATES: protein motif:HMM:NF022659.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HIR1406560.1"
                     /translation="MFWTIDRMEEGTAILLCEDGRRSALPAEQLPAGAAEGDWGRLVD
                     GAFLPDPAQTEKRRQKIRGLLKDLLG"
     gene            complement(338..1324)
                     /locus_tag="ACZPFK_09355"
     CDS             complement(338..1324)
                     /locus_tag="ACZPFK_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1406561.1"
                     /translation="MARRRGRRTKKQERLGRRVGLALAALLFAFCCVVTAGEHYGWPT
                     PRWSEVEAWFGVADAPAVVTDGDTELHFIDVGQADATLIRQGSDYALVDAGDLGGGDR
                     LVEYLRTAGVQRLRCVVMTHPHADHIGGMPEVLQNFPVDEFLLPDFELMEKLSTSDVL
                     ARTLEALKTQKENGCAVTTAARGQEVKVGAGTLKVLLAGVETDNANNLSVCVKFSADG
                     LSCLFTGDGEAAVEKELLAVEPNLRADIFQAGHHGSSTSNGKKLLAAVRPQYIVISCG
                     QDNSYGHPHDEVLRRFEQTGALICRTDQQGSVVFSVAKNGEVSVHTARESGG"
     gene            complement(1279..>1941)
                     /locus_tag="ACZPFK_09360"
     CDS             complement(1279..>1941)
                     /locus_tag="ACZPFK_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HIR1406562.1"
                     /translation="FRELVQHHISGQLKVAPEHCAPATLAAMGKPRIEVFNRFVKRFY
                     ELTKEAGKKQYLVPYLMSSHPGCTLKDAVILAEYLCDHHMKPEQVQDFYPTPGTVSTC
                     MFYTGLDPATMKPVYVAKSAEEKSLQRALLQYYEPRNRAKVRRALELAGRRDLMAKLL
                     PRSPGEEERPKARTARKGGTAQGVGKRSGRGPATKENGRRGSGKKQWRDGAAGAPKNR
                     RG"
     assembly_gap    1942..1951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1952..>2806)
                     /locus_tag="ACZPFK_09365"
     CDS             complement(<1952..>2806)
                     /locus_tag="ACZPFK_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1406563.1"
                     /translation="FAHYDYWSDTVMPSILEDSGADLLTFGMGEHQTTEIARRLAAGE
                     RPDSLTDIPGTCYLTDGAGLPAGYVECAGFAKVAADKAAYARACRIQMDNQDPLAGKP
                     VVQKQRQKYLVQNPPSKPLYRPELDKVYALPFTRTWHPSYDALGGVPGLAEVEFSIAQ
                     NRGCFGGCNFCAITLHQGRRVTSRSAESILAEGERITKSPNFKGYIHDVGGPTADFRL
                     PSCREQMKRGMCTDRKCLAPTPCPKLLVDHSEYVSILRRLRALPGVKKVFIRSGIRYD
                     YLMQDKDET"
BASE COUNT          412 a         1002 c          840 g          542 t
ORIGIN      
        1 cactctatca catccggccg gttgccccca ccaactatcg cgcaaaaatg ggttgcatcc
       61 ttggttgcgc ctcttttttt gccgggccgc gtcgtcggcc ccgccgcttt ttttcagccc
      121 cttatcccaa caggtctttc agcaggccgc gtattttctg cctgcgcttt tcggtctgcg
      181 ccgggtcggg caaaaaggcg ccgtccacca gccgtcccca gtcgccctcc gccgcgcccg
      241 cgggcagctg ttccgcgggc agcgcgctgc ggcgaccgtc ctcgcacagc aggattgccg
      301 tcccctcctc catgcggtca atggtccaga acatccctca tcccccgctt tcccgcgccg
      361 tgtgcacgct gacctcgccg tttttcgcca ccgaaaagac cacgctgccc tgctggtcgg
      421 tgcggcagat cagggccccc gtctgctcaa accgccgcag cacctcgtca tgggggtgcc
      481 catagctgtt gtcctgcccg caggagatca cgatatactg cggccgcacc gccgccagca
      541 gctttttgcc gttgctggtg ctgctgccgt gatgaccggc ttgaaagata tcggcgcgca
      601 ggttgggctc caccgccagc agctcttttt ccaccgcggc ctcgccgtct ccggtgaaga
      661 ggcaggacag gccgtccgca gaaaatttca cgcagacgga gaggttgttg gcgttgtcgg
      721 tctccacccc cgccagcagc actttcagcg tgcccgcgcc caccttcacc tcctggccgc
      781 gcgccgcggt ggtcacggcg cagccgtttt ctttttgggt cttcagcgcc tccagcgtgc
      841 gggccagcac gtcgctggtg gacagctttt ccatcagctc aaaatccggc agcaggaact
      901 cgtccaccgg aaaattctgc agcacctcgg gcatgccgcc gatgtggtcc gcgtggggat
      961 gggtcatcac cacgcagcgc agccgctgca cccccgccgt cctcagatac tccaccagcc
     1021 ggtcgccgcc gcccagatcg ccggcgtcga ccagcgcata gtcgctcccc tgccggatca
     1081 gcgtcgcatc cgcctgaccc acatcaatga aatggagctc ggtgtccccg tcggtgacca
     1141 ccgccggggc gtccgccacg ccgaaccagg cctccacctc gctccagcgc ggggtgggcc
     1201 agccatagtg ctcgcccgcc gtgacaacac agcaaaaggc gaacagcagt gccgccagcg
     1261 ccaggccgac gcgccgccct agcctctcct gttttttggt gcgcctgccg cgccgtctcg
     1321 ccattgcttt ttcccgctcc ctctccggcc attctctttt gtcgccggtc cacggccgct
     1381 gcgcttacca acgccctgcg ccgtcccgcc tttgcgggct gtgcgcgcct ttggccgctc
     1441 ctcctcccct gggctgcggg gcagcagctt cgccatcagg tcccgccgcc cggccagctc
     1501 cagcgcccgc cgcaccttgg cccgattgcg cggctcataa tactgcagca gcgcccgctg
     1561 cagggatttt tcctccgctg atttcgccac atacacgggc ttcatggtgg cggggtccag
     1621 ccccgtgtag aacatgcagg tggacacggt gcccggcgtg ggataaaaat cctgcacctg
     1681 ttcgggcttc atgtgatggt cgcagagata ttccgccagg atcaccgcgt ctttcagcgt
     1741 gcagcccggg tgactgctca tcagataggg caccagatac tgctttttgc cagcctcctt
     1801 ggtcagctcg taaaaccgct tgacaaagcg gttgaacacc tcgatgcgcg gcttgcccat
     1861 ggccgccagg gtggcggggg cgcagtgctc gggcgcaacc ttcagctgcc ccgagatgtg
     1921 atgctgcacc agttccctaa annnnnnnnn nggtctcgtc tttgtcctgc atcaggtagt
     1981 cgtagcggat gccgctgcgg atgaagacct ttttcacgcc gggcagcgcc cgcagccgcc
     2041 gcaggatcga gacgtactcg ctgtggtcca ccagcagctt cgggcagggg gtgggggcca
     2101 ggcacttgcg gtcggtacac atgccccgct tcatctgttc gcggcagctg ggcagcctga
     2161 aatccgccgt ggggcccccc acgtcgtgga tatatccctt gaaattgggg ctctttgtga
     2221 tgcgctcgcc ctccgccagg atcgactcgg cgctgcggct ggtgacccgc cgcccctggt
     2281 gcagcgtgat ggcgcagaag ttgcagccgc caaagcagcc gcggttctgg gcgatggaga
     2341 actccacctc cgccaggccc ggtacaccgc ccagggcgtc atagctgggg tgccaggtgc
     2401 gggtaaaggg cagcgcgtac accttgtcca gctccggccg gtaaagcggc ttgctgggcg
     2461 ggttctgcac cagatatttc tgccgctgct tttgcaccac cggcttgccc gccagcgggt
     2521 cctgattgtc catctggatg cggcacgccc gagcgtaggc ggccttgtcc gccgccacct
     2581 tggcaaagcc cgcgcactcc acatagcccg cgggcagccc tgcgccgtcg gtgaggtagc
     2641 aggtgccggg gatgtcggtg aggctgtcgg gccgttcccc ggccgccagc cgccgcgcga
     2701 tctcggtggt ctgatgctcg cccatgccga acgtgagcag gtctgcgccg gagtcctcca
     2761 gaatcgaggg catcaccgtg tcggaccagt aatcatagtg ggcgaa
//
LOCUS       DBMDYA010000380         2801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7888_length_2801_cov_1.105926, whole genome shotgun sequence.
ACCESSION   DBMDYA010000380 DBMDYA010000000
VERSION     DBMDYA010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_2801_cov_1.105926"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="ACZPFK_09370"
     CDS             complement(<1..660)
                     /locus_tag="ACZPFK_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR1406564.1"
                     /translation="MKKTIFLLVAFSLFSYLLTGCGSPAEDKQYDLAKVSETISSVSP
                     VPYPADMDDAYLTGMYGIDLADVITSVENAPQIPGRMEKVNPTPGAQPLVIVDYAHTP
                     EALEWTLRSTRELTPGKVVIVFGTDGDRDATKREHLAQIAAREADILWVTDENPRTED
                     PQSIRDYLLRGIASVRPGMEDVTEVTTCRRDAVRRAILAAKPGDTVIITGKGAEWYQE
                     IQ"
     gene            complement(1010..1459)
                     /locus_tag="ACZPFK_09375"
     CDS             complement(1010..1459)
                     /locus_tag="ACZPFK_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1406565.1"
                     /translation="MRKTASVIAALLMALTLFAGCGASYQDGVYHAEFKDYDSHGWKE
                     YVDVQVQDNKVTVLTFDGVNADGDKKSEDEDYRASMEPVKGTYPAKFYADITNQYLEK
                     GELKDVTTVAGATLSTDNFKALMLALESNMKDGVTDPVIVDSSASQK"
     gene            1701..2750
                     /locus_tag="ACZPFK_09380"
     CDS             1701..2750
                     /locus_tag="ACZPFK_09380"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1406566.1"
                     /translation="MTRKNKILLAAGCCAAAALVAVGVLRGLARTPHDSTTYAMSTIV
                     TQRAYGLQAEQAMRQVESTLREFEGRLSLFDQDSEIAAINAAAGDHPVRVSEATFGLL
                     RQSLALSATSEGAFDITIAPLTQLWGITTDHPRVPTQQEIDGALALVGDAEVQLDQQA
                     STVFLPRAGQAIDLGGIAKGNACVLAQRIYEDCGVKSAVLSIGGNVYVRGRNPDGEQF
                     RVGFRTPKRADESYIASVALEDQVMAVSGGYERYFEEGGVRYHHILSSETGYPAQSDI
                     VSVGVICEDGTRADFLSTTLFVWGTEKTREFMRSHDDVAVILLTEGEELLVSRSLQES
                     FRLSEQGAGYRVTFI"
BASE COUNT          522 a          853 c          914 g          512 t
ORIGIN      
        1 ttggatttcc tggtaccact cagcgccctt gcccgtaatg atgacggtgt cgccgggctt
       61 ggccgccagg atggcgcggc gcacggcgtc gcgtcggcac gtcgtaacct cggtgacgtc
      121 ctccatgccg gggcgcacgg aggcgatgcc acgcagcaga tagtcacgga tagactgagg
      181 atcctcggtg cgcgggttct cgtccgtgac ccacaggatg tcggcctcgc gtgccgcgat
      241 ctgcgccaaa tgctcacgct tggtcgcgtc gcggtcgccg tcggtgccaa agacgatgac
      301 gactttgccg ggggtcagct cgcgcgttga gcgcagcgtc cactcgagag cctcgggggt
      361 gtgcgcgtag tcgacgatca cgaggggctg ggcaccgggg gtcgggttga ccttctccat
      421 gcgtccgggg atctggggtg cattttcgac ggacgtgatg acgtcggcca ggtcgatgcc
      481 gtacatgccg gtgagatacg cgtcgtccat gtcggcggga tagggcaccg gcgagaccga
      541 ggagatggtt tcggacacct ttgcaagatc atattgcttg tcctctgcgg gcgagccgca
      601 gcccgtcagc aggtaggaaa agagggaaaa agccaccagc agaaaaatcg tctttttcat
      661 aaaacgcctc tccaaatttt tcatctcata ccagtatatc ctaactgaat ggaggcgatt
      721 tttcaagagg cacccatagt gttcgcacaa aagttaccaa ttattcacga atctttccag
      781 ccccgcgggg cggcaaaagc gcagcgcgca gcggaacaaa gcgtcctctg ccgcagcgga
      841 cagaaacacc ccccgatccg gggaacaggc cgcgggcgga cggcaaaggg cagcaaaaaa
      901 gcccgcggcc ggcgcgggct caaaggccgg cggcgccggc ctgatgcagc gaaaaaatcc
      961 agggcgcccg gcgaaagcct gtcggcgccg aacggcagcc ttgccgccgc tatttttgtg
     1021 aggcagagga atccacgatc accgggtcgg tgacgccgtc tttcatgttg gactccagcg
     1081 ccagcatcag cgctttgaag ttgtcggtgg acagggtcgc gcccgccaca gtggtgacgt
     1141 ctttcaactc gcctttttcc agatactggt tggtgatgtc ggcgtagaat ttggccggat
     1201 aggtgccctt gaccggctcc atcgaggcgc ggtagtcctc atcctccgac tttttgtccc
     1261 cgtcggcgtt cacgccgtcg aacgtgagga cggtgacctt gttgtcctgc acctggacgt
     1321 cgacatattc tttccagccg tggctgtcat agtccttgaa ctccgcgtgg tagacgccgt
     1381 cctgataaga ggcgccgcag cccgcaaaca gggtgagcgc catcagcagc gccgcgatca
     1441 cagacgccgt ttttctcatt cgcatttcac ctcccatccg cgtacgcgca actcattata
     1501 ataaaattcg cctatgctgt caaatttagc gggattttgt cacggaacag ggcgtcccag
     1561 gggaacaagt gcatggcacc accccacggc tgcttgacaa agggaggctt tcctcctaaa
     1621 attgtactct cagagcgccg cttttccgcc ttggcgcgag agggcggccc agatcaatct
     1681 tttgacgaca ggatgggacg atgacacgaa agaacaagat ccttttggcg gcggggtgct
     1741 gcgcggccgc ggcgctcgtc gccgtggggg tgctgcgcgg cctggcgcgc acaccgcacg
     1801 attccaccac ctatgccatg agcaccatcg tgacccagcg cgcttacggc ctgcaggccg
     1861 agcaggcgat gcgacaggtg gaaagcaccc tgcgggaatt tgagggcagg ctctccctgt
     1921 tcgaccagga cagcgagatc gcggcgatca acgcggcggc gggggatcac cccgtccggg
     1981 tgagcgaggc cacctttggc ctgctgcggc agagcctggc cctctcggcc acctcagagg
     2041 gggcgttcga catcaccatc gcccccctca cccaactgtg gggcatcacc accgatcatc
     2101 ccagggtgcc tacccagcag gagatcgacg gcgccctggc cctggtcggc gacgcggagg
     2161 tgcagctgga ccagcaggcc agcaccgtct ttttgccccg cgccggacag gccatcgacc
     2221 tgggcgggat cgccaagggc aacgcctgtg tgctggccca gcgcatctat gaggactgcg
     2281 gcgtcaaatc ggcggtgctg agcattggcg gcaacgtcta tgtgcggggc agaaaccccg
     2341 acggagagca gttccgggtc ggattccgca ccccgaaaag ggcggatgaa tcctatatcg
     2401 cctcggtggc gctggaggat caggtcatgg ctgtgtcggg cggctacgag cggtattttg
     2461 aggagggggg cgtccgctac caccacatcc tgtccagcga gaccggctac ccggcccaga
     2521 gcgacatcgt ctcggtgggg gtcatctgcg aggacggcac ccgggcggac tttttgtcca
     2581 ccaccctgtt cgtgtggggg acggagaaga cgcgggagtt catgcgcagc cacgacgacg
     2641 tggcggtcat cctgctcacc gagggggagg agctgctggt gtcccgctcg ctgcaggaga
     2701 gctttcgcct cagcgagcag ggggcgggct atcgcgttac ctttatataa ccgaagttgg
     2761 cggcggccgg gccgcccggc aggcggcagg ggagcagggc g
//
LOCUS       DBMDYA010000381         2798 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7898_length_2798_cov_1.466073, whole genome shotgun sequence.
ACCESSION   DBMDYA010000381 DBMDYA010000000
VERSION     DBMDYA010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7898_length_2798_cov_1.466073"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(79..324)
                     /locus_tag="ACZPFK_09385"
     CDS             complement(79..324)
                     /locus_tag="ACZPFK_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1406567.1"
                     /translation="MKSTGIVRKVDDMGRIVLPIELRRAMEIEDGSPLEIYVEEDSIV
                     LKACKPVCIFCGSDQSVEEFKGRCVCASCRGELRGGK"
     gene            complement(552..1643)
                     /locus_tag="ACZPFK_09390"
     CDS             complement(552..1643)
                     /locus_tag="ACZPFK_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406568.1"
                     /translation="MKKFLRVLALFLLPVALLYGLFAAVLVSTRELAGMEEIVAATLD
                     GSLTLYGTSHHENFATYKLRVTSALAPRLLVLGTSRSMQLRGEFFSEKSFYNAGGAVR
                     NMADYEDFLRRLPEEALPETLLVVLDQNMFNTNWRQSSPTAPQDYGELEMDFFDTLLR
                     TGLDYGNRKFSIAKTILPQQGVYGLAAAARGMGFAVDGSYRYGKTAEKNLDQPEKNFA
                     DTYRNIDFGELRFAYGEAPDALALSQLDGLLAFCAEKEIRVVGFLPPFPPAVNRRMEQ
                     NGGYGYLDTLYEEVARRFAGYGWEFYDFTAVDSADDEYLDGFHGGDRVYAKIALNLSG
                     NSEILSKNIDSATLEKLLAERGGNPRILP"
     gene            complement(1712..1984)
                     /locus_tag="ACZPFK_09395"
     CDS             complement(1712..1984)
                     /locus_tag="ACZPFK_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406569.1"
                     /translation="MSHLFTGFVPTIAHLKESLVLLGLSAEACVRFAVLFVALFAVDL
                     AARDRGWRAWLSARRPWVQAAVCYLLVAALVFWGNLGGGNSMYFKF"
     gene            complement(1981..>2798)
                     /locus_tag="ACZPFK_09400"
     CDS             complement(1981..>2798)
                     /locus_tag="ACZPFK_09400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1406570.1"
                     /translation="QPVGVSYYTFQMISYLVDVYRQKLAPEKNFFACSVSLSLFLQIT
                     SGPLTRPRDLLPQLRQERAFDTARGIASAQLVLLGLFKKIVVADALAYYTKAVYADPT
                     RLYGLSLMVAALFYTVQIYADFSGYSDMMRGFGGLLGLELAENFDAPYLSRSVKEFWK
                     RWHISLSGWLIEYVYIPLGGNRVSRPRYYFNLFFTFVLSGLWHGASAPMLVWGALHGA
                     YRVAGAATDGLRLRAYRALRLRRGCGRRWSPLCWWGLPGSFSARPISPRRGIS"
BASE COUNT          613 a          882 c          763 g          540 t
ORIGIN      
        1 aataaaaaac aatttatctt gatggccccg gccagaggac tggccggatg gccgtaaaaa
       61 atagcctgcg gctatttttt atttgccccc gcgcagctcg ccgcggcaag aggcgcagac
      121 acagcggccc ttgaactcct ccacactctg gtcgctgccg cagaaaatac agaccggctt
      181 gcaagccttg aggacgatgc tgtcctcctc tacatagatc tccagcggcg agccatcttc
      241 aatctccatg gcgcggcgca actcgatggg cagcacaata cggcccatgt cgtcaacctt
      301 gcgaacaatt ccagtggatt tcatctaagt aaaactccct ccctcattca tgtgtgattc
      361 cattttttgt gaatacttct ccatctttca gaaaaacgat tcccgttctc gacaaaatca
      421 tttttcttac acgtttaaaa taccatatcc ccccttatat gtcaataatt tccagttgga
      481 aaatattggt ttttttatct ttttttgtga cttaaaaaaa cgtttcccca tcattcgcga
      541 aatattgtct tttatggcag gattcgcgga tttccgcctc tttcggcaag caatttttct
      601 aaagtcgcag aatctatatt ttttgacaaa atctcgctgt ttcccgaaag attcaacgcg
      661 attttcgcgt agacacggtc cccgccgtga aagccgtcca ggtattcgtc gtccgcgctg
      721 tccaccgctg tgaaatcgta aaattcccat ccatatcccg caaagcgccg ggcgacttct
      781 tcatagagag tgtccagata gccgtatccc ccgttctgct ccatgcgtcg gttgaccgcg
      841 ggggggaacg gcggcagaaa ccccaccacc cggatctcct tttccgcgca aaaggccagc
      901 aggccgtcca gctgcgagag ggccagcgcg tccggcgcct cgccataggc aaagcgcagc
      961 tcaccgaaat cgatgttgcg gtaggtatcg gcaaagtttt tttccggctg gtccaggttt
     1021 ttttcagccg tcttgccgta gcgatagctg ccgtcgaccg caaagcccat gccgcgggcg
     1081 gcggcagcca ggccgtaaac gccctgctgg ggcaaaatgg tctttgcgat cgaaaacttg
     1141 cggttgccgt agtccaggcc ggtgcgcagc agcgtgtcga aaaaatccat ctccagctcg
     1201 ccgtagtcct gcggggccgt ggggctgctc tgccgccaat tggtgttgaa catgttctgg
     1261 tcgagcacca ccagcagggt ctcgggcagc gcctcctccg gcaagcggcg caaaaagtcc
     1321 tcataatcgg ccatattgcg caccgcgccg ccggcgttgt aaaagctctt ttcagaaaaa
     1381 aactctccgc gcagctgcat gctgcggctg gtgcccagca ccagcaggcg cggcgccagc
     1441 gcgcttgtca cccgcagctt gtaggtggca aagttttcgt ggtgcgaggt gccgtacaac
     1501 gtgaggctgc cgtccagcgt ggcggcgacg atctcctcca tgccggccag ctcgcgggtg
     1561 ctcaccagca ccgcggcgaa cagcccgtac agcagcgcca ccggcagcag gaacagcgcc
     1621 agaacgcgaa gaaacttttt cattttgccg tcccctctct cccgtttttt ctcgccgcac
     1681 ggggcccgcg ccgaaagccg tgcccgccct ctcaaaattt gaaatacatc gagttgccgc
     1741 cgccgaggtt gccccagaac accagcgcgg ccaccagcag atagcacacc gccgcctgca
     1801 cccacggccg ccgggccgag agccaggcgc gccagccccg gtccctggcg gcaaggtcca
     1861 ctgcgaacag ggcgacaaac agcacagcaa agcgcacaca ggcctcggcg gagagcccca
     1921 gcagcaccag gctctctttc aggtgggcga tggtgggcac aaagccggtg aacagatgac
     1981 tcacgatata ccacgccgtg gcgagattgg gcgcgccgaa aaagatccag gcaaacccca
     2041 ccagcacaaa ggtgaccagc gtctgccaca gccgcggcgc aagcgcagcg cccggtaggc
     2101 ccgcaggcgc agcccgtcgg tggcggcccc cgccacccgg tacgccccgt gcagcgcccc
     2161 ccacaccagc atgggcgcac ttgcgccgtg ccagaggccg ctgagcacaa aggtgaagaa
     2221 cagattgaaa tagtagcgcg ggcggctcac ccggttgcct cccagcggga tatagacgta
     2281 ttcaatcagc cagccggaaa gggagatgtg ccacctcttc cagaattctt tcaccgagcg
     2341 ggagagatag ggcgcgtcaa aattttcagc cagctccagc cccagcagcc cgccgaagcc
     2401 ccgcatcata tcgctgtagc cggaaaaatc cgcgtaaatc tgcacggtgt agaacagcgc
     2461 cgccaccatc aaactgaggc catacaggcg ggtggggtcg gcgtagacag ctttggtgta
     2521 ataggccagc gcgtcggcca ccacgatctt tttgaagagg cccagcagca ccagctgggc
     2581 cgaggcgatg ccccgcgccg tgtcaaaggc ccgctcctgc cgcagctggg gtaggaggtc
     2641 ccgtgggcgg gtgagagggc ccgaggtgat ctgcaaaaac agggataggc tcaccgagca
     2701 ggcaaaaaag tttttttccg gcgcgagctt ttgccggtag acatccacca gatagctgat
     2761 catctgaaag gtgtaatagc tcacccccac cggctgca
//
LOCUS       DBMDYA010000382         2797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7901_length_2797_cov_1.685460, whole genome shotgun sequence.
ACCESSION   DBMDYA010000382 DBMDYA010000000
VERSION     DBMDYA010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_2797_cov_1.685460"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..775)
                     /locus_tag="ACZPFK_09405"
     CDS             complement(167..775)
                     /locus_tag="ACZPFK_09405"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1406571.1"
                     /translation="MSKRVITISREFGSGGRAIGKMVADKLGIKFYDRELIQLVAKES
                     GFALDFVEETGEYSSTSSLLYNLSVGGIYTQGAFSPETLPPADKVQILQNNIIRDLAE
                     KEPCVIVGRSADYILRERSDCLNVFIHADMEHRVKRAVELYNISPKSPEKILAKKDKA
                     RAGQYRRYTDQVWGMADNYNLTLDSGMFGLEKCCDIIVALAK"
     gene            1028..1861
                     /locus_tag="ACZPFK_09410"
     CDS             1028..1861
                     /locus_tag="ACZPFK_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406572.1"
                     /translation="MVAFGSVAVFSRAADSIGGLRGTIIFYSVLLLVAGVLSMIFIPN
                     NKPEKVVQKAEAKESAPLKWKNFLEVAKMPRVWLCGVLVVCNYAALIFHGYVTPYLSQ
                     AFGLSDSTVATLSVIRTYFMMMVGAVAAGFIADKVGSRIKFIQWAFVGMTVFAACYLL
                     IPANRGAVWVIVANFIIYGLCLYSIKALYFSTIDEVLIPKRLAGTASGIISLVGYAPE
                     IFLYTVSGNMVDKAEGLNGYKLCFLWMVILSAIGFICAIVLRRLNAKAIEKAKLEGAP
                     V"
     gene            1895..>2797
                     /locus_tag="ACZPFK_09415"
     CDS             1895..>2797
                     /locus_tag="ACZPFK_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406573.1"
                     /translation="MSEQLLSSVNSDYNNDLHNWAEIERQVTNIAKAGFTHTQWIHDW
                     EGEYLYSASEMFQVRDLLRGVGVRAHSIHASEGGRRTIRVNGRPVFRNRTRLADIRKD
                     YTSLNEYTRLAGVELLKNRVDLCSHIGASVMVLHMQLPYEMFEKNPADKEDYYRQVCK
                     SFDELRDYAKAAGVRVALENLICTPPRHQEEEFERMFDRYDEDFLGFCFDSGHGSLMC
                     RDNYYYFLEKYYKRMYATHLQDNDSISEELAGDDSEVLLHDKHRPPFTGVLDWEKIAD
                     YVAKAPLQLPADFEVGLYGDTPEEE"
BASE COUNT          569 a          844 c          835 g          549 t
ORIGIN      
        1 aaagcggcgc tgatcccacc ccccctttgc ccgggagatg gggaccaacg ccgcttttgc
       61 gacaatagaa acgggacggc ggaaaaacaa cctatcccaa gggcgccgtt tgccgccgct
      121 gccgcggcaa acgaagcaca gggctaaaac cgtctgcggc cgcctttcat tttgccagcg
      181 ccacgatgat atcgcagcac ttttccagcc cgaacatgcc gctgtccagc gtcaggttgt
      241 agttgtccgc catgccccag acctggtcgg tgtagcgccg gtactggccc gcccgcgcct
      301 tgtctttttt ggcgaggatc ttttcaggcg actttggcga gatgttgtac agctccaccg
      361 cccgttttac ccggtgctcc atgtcggcgt ggataaaaac gttcaggcag tcgctgcgct
      421 cgcgcaggat atagtccgcc gagcggccca cgatcacaca gggctctttt tccgccaggt
      481 cgcggatgat gttgttctgc aggatctgca ccttgtcggc cgggggcagg gtctcgggcg
      541 aaaacgcgcc ctgggtgtag atgccgccca ccgacaggtt gtacagcagg ctgctggtgg
      601 aggaatactc cccggtctcc tcgacaaagt ccaaggcgaa gccgctctct ttcgccacca
      661 gctggatcag ctcgcggtca taaaacttga tgcccagctt atccgcgacc atcttgccga
      721 tcgcgcgccc gccgctgcca aactcgcggc tgatggtgat gacccttttg gacatacaac
      781 gaacctccgt ttccgaaaag cggacagact gtgcagagtg ccgaatgaaa aggcctgcgc
      841 cgctttgttt ttgtactttt attataactg caaacgcttc gctcggcaag ccgtcccggg
      901 agcaaaattt tcagaaaaaa gaaaaataca caacacagct tgtccacaac cagacaaaat
      961 gaacaaaatg tcaaataaaa gtgaataaat tgttgacaaa gagggcggcc gcggcctcat
     1021 cggcacactg gtcgctttcg gctcagtggc ggtcttctcc cgcgcggccg acagcatcgg
     1081 cggcctgcgg ggcaccatca tcttctacag cgtgctgctg ctggtcgcgg gcgtgctgtc
     1141 gatgatcttc atccccaaca acaagccgga aaaagtggtg cagaaagcgg aggccaaaga
     1201 gagcgccccc ctgaagtgga agaactttct cgaggtggcc aaaatgccgc gggtctggct
     1261 gtgcggcgtg ctggtggtct gcaactacgc ggccctcatc ttccacggct atgtcacccc
     1321 gtacctgtcg caggcgttcg gcctgagcga ttccaccgtg gccaccctca gcgtcatccg
     1381 cacctatttc atgatgatgg tgggcgccgt ggcggccggc ttcatcgccg acaaggtggg
     1441 cagccgcatc aagttcatcc agtgggcttt cgtgggtatg accgtctttg ccgcctgcta
     1501 tctgctgatc ccggccaacc ggggcgcggt ctgggtgatc gtggccaact tcatcatcta
     1561 cggcctgtgc ctgtacagca tcaaggcgct gtatttctcc accatcgacg aggtgctgat
     1621 ccccaagcgt ctggcgggca ccgcctccgg catcatctcg ctggtgggct atgcgcctga
     1681 gatcttcctc tacacggtca gcggcaacat ggtcgataag gccgagggcc tcaacggcta
     1741 caagctgtgc ttcctgtgga tggtcatcct ctccgccatc ggctttatct gcgccattgt
     1801 gctgcggcgg ctgaacgcga aggccatcga gaaagcgaaa ctagagggcg cgccggtctg
     1861 aggccgggcg ctgcaaaaaa caggaggcga gaccatgtca gagcaattgc tcagctccgt
     1921 caacagcgac tacaacaacg atttgcacaa ctgggccgag atcgagcgcc aggtgaccaa
     1981 catcgccaag gcgggcttta cccacaccca gtggattcac gactgggagg gggagtatct
     2041 ttacagcgcc agtgagatgt tccaggtgcg cgacctgctg cgcggcgtcg gggtcagggc
     2101 gcacagcatc cacgcgtccg agggcggccg ccgcaccatc cgggtgaacg gcaggccggt
     2161 gttccgcaac cgcacccggc ttgcggatat ccgcaaggac tacacctccc tcaacgagta
     2221 cacccgcctg gccggtgtgg agctgctgaa aaaccgggtg gatctgtgca gccatatcgg
     2281 cgcctcggtg atggtgctgc acatgcagct gccctatgag atgttcgaga agaaccccgc
     2341 cgacaaagag gactattacc gccaggtctg caagtccttt gacgagctgc gggattacgc
     2401 caaggccgcc ggggtgcggg tggcgctgga gaacctgatc tgcacgccgc cccgccatca
     2461 ggaggaggag tttgagcgga tgtttgaccg ctacgacgag gactttttgg gcttctgctt
     2521 cgattcgggc cacggcagcc tgatgtgccg cgacaattac tattactttt tggaaaagta
     2581 ttacaagcgg atgtacgcca cccatctgca ggacaacgac tcgatcagtg aggagctggc
     2641 cggggacgac agcgaggtgc tgctgcacga caagcaccgg ccgcctttca ccggtgtgct
     2701 ggactgggag aagatcgccg attatgtggc gaaagcgccg ctgcagctgc ccgcggattt
     2761 cgaggtgggc ctttacggag acaccccgga ggaggag
//
LOCUS       DBMDYA010000383         2794 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7909_length_2794_cov_1.438173, whole genome shotgun sequence.
ACCESSION   DBMDYA010000383 DBMDYA010000000
VERSION     DBMDYA010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_2794_cov_1.438173"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="ACZPFK_09420"
     CDS             <1..1051
                     /locus_tag="ACZPFK_09420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR1406574.1"
                     /translation="TMTQHQLAKWCDADIIVYVGCGERGNEMTQVLEEFGELVDPKSG
                     KPMTERTVLIANTSNMPVAAREASIYTGVTLAEYYRDMGYDVAMMADSTSRWAEALRE
                     ISGRLEEMPAEEGFPAYLPSRLAEFYERAGCTENLNGSSGSVTLIGAVSPQGSDFSEP
                     VTQNTKRFVRCFLALDKALAYARHYPAINWNISYSEYYEDLDPWYIKNVGPEFCALRG
                     RLLSLLSEESKLMEIVKLIGSDVLPDSQKLTIEIAKVIRTGFLQQNAFHKDDTYVPLV
                     KQMKMMEVILYLQKTGAALVAEGIPMSALLGTGLFERLVKMKYDVPNDRPELFKGYYT
                     EIDDAAKRLRAEHIA"
     gene            1068..2456
                     /locus_tag="ACZPFK_09425"
     CDS             1068..2456
                     /locus_tag="ACZPFK_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR1406575.1"
                     /translation="MAIEYLGLSEINGPLVALDGVEGASFEEMVEIRQKDEKRLGRVV
                     ALEGDRAIVQVFDGTSGLGLASTSTRFTGYPMRLALAPEIVGRVFNGAGQPIDGLGPV
                     YAEKFEDINGSPINPVSRTYPRNYIRTGISSIDTLMTLIRGQKLPIFSGSGMSHNKLA
                     AQIVRQAKLSDKDGENGEKFGVVFCAMGVTNDVADYFRRSFSESGAESRVTMFLNLSS
                     DPIIERILTPRCALTAAEYLAFEQDMHVLVVMTDMTSYAEALREFSSSKGEIPGRKGY
                     PGYLYSDLASLYERAGIVKGRGGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDR
                     YLDQTGVYPPIDVLPSLSRLMKDSIGEGYTRADHSDLYNQLFASYAKVSDARGLASVI
                     GEEELSPTDKQYLQFGKEFEQRFLNQGAGEDRTIDQSLDLGWELLSILPREELDRLDD
                     KVIEEHYNKHSA"
     gene            2527..>2794
                     /locus_tag="ACZPFK_09430"
     CDS             2527..>2794
                     /locus_tag="ACZPFK_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1406576.1"
                     /translation="MAENVIPTKGNLISLKRSLAQAKLGFELLDRKRNVLMREMMKLI
                     DESAKLQQDIGDTFSSAYKALQAANVTLGIDPIMIGTEPVENGLS"
BASE COUNT          552 a          925 c          870 g          447 t
ORIGIN      
        1 aaccatgacc cagcaccagc tggccaaatg gtgcgacgcc gacatcatcg tctatgtggg
       61 ctgcggcgag cgcggcaacg agatgaccca ggtgctggag gagttcggcg agctagtgga
      121 ccccaagagc ggcaagccga tgaccgagcg cacggtgttg atcgccaaca ccagcaacat
      181 gccggtggcg gcccgcgagg cctccatcta caccggcgtc accctggccg aatactaccg
      241 cgacatgggc tacgacgtgg ccatgatggc cgattccacc tcgcgctggg ccgaggccct
      301 gcgtgagatc tcgggccggc tggaggagat gcccgccgag gagggcttcc ccgcctatct
      361 gcccagccgc ctggccgagt tctacgagcg ggccggctgc accgaaaacc tgaacggctc
      421 ctccggcagc gtgaccctga tcggcgcggt ctcgccccag gggtcggatt tttcagagcc
      481 ggtgacccag aataccaagc gctttgtgcg ctgttttttg gcgctggaca aggcgctggc
      541 ctatgcccgc cactacccgg ccatcaactg gaacatcagc tacagcgagt actatgagga
      601 tctggatccc tggtacatca aaaacgtggg ccccgagttc tgcgccctgc gcggccggct
      661 gctgagcctg ctcagcgagg aatcgaagct gatggagatc gtcaagctga tcggctccga
      721 cgtgctgccg gacagccaga agctgaccat cgagatcgca aaggtcatcc gcaccggctt
      781 tttgcagcag aacgcctttc acaaggacga cacctatgtg cccctggtca agcagatgaa
      841 gatgatggag gtcatcctct acctgcaaaa gaccggcgcc gcgctggtgg cggagggcat
      901 ccccatgagc gccctgctgg gcaccggcct gtttgagcgg ctggtcaaga tgaaatacga
      961 cgtgcccaac gaccggcccg agctgttcaa gggctattac accgagatcg acgacgcggc
     1021 caagcgcctg cgcgccgagc acatcgccta gagaggagag attccaaatg gcaattgaat
     1081 atctgggtct ttccgagatc aacggccccc ttgtcgccct ggacggggtg gagggcgcct
     1141 cgtttgaaga gatggtggag atccgccaga aagacgaaaa gcgcctgggc cgcgtggtgg
     1201 cgctggaggg ggaccgggcc atcgtacagg tgttcgacgg cacctcgggc ctggggctcg
     1261 cctccaccag cacccgcttt accggttacc ccatgcgcct ggcgctggcc cccgagatcg
     1321 tgggccgcgt cttcaacggc gcgggccagc ccatcgacgg cctgggcccg gtctacgccg
     1381 aaaaatttga ggacatcaac ggcagcccca tcaacccggt cagccgtacc tatccccgca
     1441 actacatccg caccggcatc tccagcatcg acacgctgat gaccctgatc cgcggccaga
     1501 agctgccgat tttttccggc agcggcatga gccacaacaa gctggccgcc cagatcgtgc
     1561 ggcaggcgaa gctctcggac aaggacggtg aaaacgggga aaaattcggc gtggtcttct
     1621 gcgccatggg cgtcaccaac gacgtggccg actatttccg gcgcagcttt tccgagagcg
     1681 gcgccgagag ccgggtcacc atgtttttga acctctcctc cgaccccatc atcgagcgca
     1741 tcctcacccc gcgctgcgcg ctgacggcgg cggagtacct ggcctttgag caggatatgc
     1801 acgtgctggt ggtcatgacc gacatgacca gctatgccga ggccctgcgc gagttctcct
     1861 cctccaaggg cgagatcccc ggccgcaagg gttatcccgg ctatctctac tcggacctcg
     1921 ccagcctcta cgagcgggcg ggcatcgtca agggccgcgg cggcagcgtc acccagatcc
     1981 ccatcctgac catgcccaac gacgacatca cccaccccat cccggacctg accggctata
     2041 tcaccgaggg gcagatcgtg ctggaccggt atctggacca gaccggcgtc tatccgccca
     2101 tcgacgtgct gcccagcctg tcccgtctaa tgaaggacag catcggcgag ggctacaccc
     2161 gcgccgacca ctcggacctg tacaaccagc tcttcgccag ctacgccaag gtcagcgacg
     2221 cgcgcggcct cgccagcgtc atcggcgagg aggagctctc ccccaccgac aaacagtacc
     2281 tgcaattcgg caaggagttt gagcagcggt ttttgaacca gggcgcgggc gaggaccgca
     2341 ccatcgacca gagcctggac ctgggctggg agctgctgtc catcctgccg cgggaggagc
     2401 tggaccgcct ggacgacaag gtcattgagg agcactacaa caagcactcg gcctgagggc
     2461 gcggggcgcg ggcggccggt ccgccccgcc ccgcgcgccc ggccgttctg aaaggagggg
     2521 gccaacgtgg ctgagaacgt catccccacc aagggcaacc tgatcagcct caagcgcagc
     2581 ctggcgcagg caaagctggg ctttgagctg ctggaccgca agcgcaacgt gctgatgcgg
     2641 gagatgatga agctgatcga cgagtccgcc aagctgcagc aggatatcgg ggacactttt
     2701 tcctccgcct ataaggcgct gcaggcggcc aacgtgacgc tgggcatcga cccgatcatg
     2761 atcggcaccg agccggtgga aaacgggctc tcgc
//
LOCUS       DBMDYA010000384         2776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7959_length_2776_cov_1.156262, whole genome shotgun sequence.
ACCESSION   DBMDYA010000384 DBMDYA010000000
VERSION     DBMDYA010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7959_length_2776_cov_1.156262"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="carA"
                     /locus_tag="ACZPFK_09435"
     CDS             <1..816
                     /gene="carA"
                     /locus_tag="ACZPFK_09435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR1406577.1"
                     /translation="LEENGIPGIQGVDTRALTRIIRSEGSQKVLLCEPYLSHDEAMAK
                     LRKARLPRDQVARVSCKKRWYSRTFNPRFDVVAVDCGIKLNIIRKLNERGCNITVVPY
                     DTTADEILALRPDGLFLSNGPGDPEDVPQVIELVRALRGRLPIFGICLGHQLISLAYG
                     AKTYKMKFGHRGGNHPVKNLDTGRIEITSQNHSFAVDAASLEGTGLTLTHVNLLDGTA
                     EGVRSDADRLFSVQYHPESAPGPQDSAYLFDEFLALMERDKQEKEQREGNKNA"
     gene            809..>2776
                     /gene="carB"
                     /locus_tag="ACZPFK_09440"
     CDS             809..>2776
                     /gene="carB"
                     /locus_tag="ACZPFK_09440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HIR1406578.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDTAIADKVYMEPLNLEYVAKIIRYERPDAIVPGLGGQTGLNLAVQL
                     AKKGVLDECQVEILGTRFESIEQAEDREKFKELCESLGEPVLPSIIAESMEQGLAAAA
                     EIGYPVVLRPAFTLGGTGGGFADDEAEFREVMKNALKLSPVHQVLVEKSIKGYKEIEY
                     EVMRDKNDTAITICNMENIDPVGIHTGDSVVVAPSQTLTNKEYQLLRDSALRLIRALK
                     IEGGCNVQFALDPHSFKYYLIEVNPRVSRSSALASKASGYPIARVSAKIAVGMTLDEI
                     AIANTPASFEPALDYVVTKIARFPFDKFATASNRLSTQMKATGEVMSIGRTIEESLLK
                     AIRSLETGVWHLHSKKFDGTAADELLRYVREGTDDRLFAIAELIRQGVDLGLIYNATK
                     IDLLFLEKFKNIVDFEKVLAAHPKDAVVLRDAKRMGVSDRYIAERWGMTEAAVYKLRK
                     KLGVFPVYKMIDTCASEFDSYVPYFYSTYEEENESVVSDKKKIVVLGSGPIRIGQGVE
                     FDYSTVHAIWGIREAGYEAIIINNNPETVSTDYTTSDKLYFEPLTVEDVMNVLELEKP
                     LGVVASLGGQTAINLAGPLHELGVKIIGTGVE"
BASE COUNT          579 a          878 c          894 g          425 t
ORIGIN      
        1 ctggaggaga acggcatccc cggcattcag ggcgtcgaca cccgggcgct gacccgcatc
       61 atccgcagcg agggcagcca gaaggtgctg ctctgcgagc cctacctcag ccacgacgag
      121 gcgatggcaa agctgagaaa agcaaggctg ccccgcgacc aggtggcgcg ggtgagctgc
      181 aaaaagcgct ggtattcccg cacgttcaac ccgcgcttcg acgtggtggc ggtggactgc
      241 ggcatcaagc tgaacatcat ccgcaagctg aacgagcggg gctgcaacat cacggtggtg
      301 ccctacgaca ccactgcgga cgagatcctg gccctgcggc cggacgggct gttcctctcc
      361 aacgggccgg gcgaccccga ggatgtgccc caggtcatcg agctggtgcg cgcgctgcgc
      421 ggccggctgc ccatcttcgg catctgcctg ggccaccagc tcatcagcct cgcctacggc
      481 gccaagacct ataagatgaa gttcggccac cggggcggca accacccggt gaaaaatctg
      541 gataccggca ggatcgagat caccagccag aaccacagct ttgccgtgga cgcggcttca
      601 ctggagggca ccggcctgac cctgacccat gtgaacctgc tggacggcac cgccgagggg
      661 gtgcgcagcg acgcggaccg gctgttcagc gtgcagtacc acccggagag cgcccccggc
      721 ccccaggact cagcctatct cttcgacgag tttctggcgc tgatggagcg ggacaagcag
      781 gagaaagagc agagggaggg gaacaagaat gcctaagcgc accgacatcc gcaaaatcat
      841 ggtcattggc agcggcccca tcgtcatcgg ccaggcggcc gagttcgact acgccggcac
      901 ccaggcctgc ctggccctga aagaggaggg ctacgaggtg gtactggtca actcgaaccc
      961 cgccaccatc atgaccgaca ccgccatcgc cgacaaggtc tacatggagc ccttgaatct
     1021 ggagtatgtg gcgaagatca tccgctacga gcgcccggac gccatcgtgc cggggctggg
     1081 cggccagacc ggcctgaacc tggcggtgca gctggccaaa aaaggcgtgc tggacgagtg
     1141 ccaggtggag attttgggca cccgctttga gagcatcgag caggccgagg accgggaaaa
     1201 attcaaggag ctgtgtgaat cgctgggcga gccggtgctg cccagcatca tcgccgaaag
     1261 catggagcag ggccttgcgg ccgcggccga gatcggctac ccggtggtgc tgcgcccggc
     1321 ctttacgctg gggggcaccg gcggcggctt tgccgacgac gaggccgagt ttcgcgaggt
     1381 gatgaaaaac gccctcaagc tgtcgcccgt gcaccaggtg ctggtggaaa agagcatcaa
     1441 gggctataag gagatcgagt atgaggtgat gcgggacaaa aacgacaccg ccatcaccat
     1501 ctgcaatatg gagaacatcg acccggtggg catccacacc ggcgactcgg tggtggtcgc
     1561 ccccagccag accctgacca acaaggaata ccagctgctg cgcgactcgg cgctgcggct
     1621 gatccgggcc ctcaagatcg agggcggctg caacgtgcag ttcgcgctgg acccccactc
     1681 cttcaaatat tacctgatcg aggtcaaccc gcgcgtctcc cgctcgtcgg cgctggccag
     1741 caaggccagc ggctacccca tcgcccgggt gtcggccaag atcgcggtgg gcatgacgct
     1801 ggacgagatc gccatcgcca acacgccggc cagctttgag cccgcgctgg actatgtggt
     1861 gaccaagatc gcccgcttcc cgttcgacaa gttcgccacc gcctcgaacc ggctctccac
     1921 ccagatgaaa gccaccggcg aggtgatgag catcggccgc accatcgagg agagcctgct
     1981 gaaggccatt cgcagcctgg agaccggggt gtggcacctg cacagcaaaa aatttgacgg
     2041 caccgccgcc gatgagctgc tgcgctatgt gcgcgagggc accgacgacc ggctgttcgc
     2101 catcgccgag ctgatccgcc agggggtgga cctgggcctc atctacaacg ccaccaagat
     2161 cgacctgctg tttttggaaa agttcaagaa catcgtcgat tttgaaaagg ttctggcggc
     2221 gcatccgaaa gatgcggtgg tcctgcggga cgcaaagcgg atgggcgtca gcgacaggta
     2281 tatcgccgag cgctggggca tgaccgaggc ggcggtgtac aagctgcgca aaaagctggg
     2341 cgtcttcccg gtatacaaga tgatcgacac ctgcgcctcc gagttcgaca gctacgtgcc
     2401 ctatttttac tccacctatg aagaggagaa cgagtccgtc gtctcggaca agaaaaagat
     2461 cgtggtgctg ggcagcgggc ccatccgcat cggccagggg gtggagttcg actactccac
     2521 cgtgcacgcc atctggggca tccgcgaggc cgggtacgag gccatcatca tcaacaacaa
     2581 ccccgagacc gtctccaccg actacaccac cagcgacaag ctgtactttg agccgctcac
     2641 cgtcgaggac gtgatgaacg tgctggagct ggaaaaaccg ctgggcgtgg tggcctcgct
     2701 gggcggccag accgccatca acctggccgg gcccctgcac gagctggggg tgaagatcat
     2761 cggcaccggc gtggag
//
LOCUS       DBMDYA010000385         2768 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_7985_length_2768_cov_1.298838, whole genome shotgun sequence.
ACCESSION   DBMDYA010000385 DBMDYA010000000
VERSION     DBMDYA010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7985_length_2768_cov_1.298838"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            337..864
                     /locus_tag="ACZPFK_09445"
     CDS             337..864
                     /locus_tag="ACZPFK_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR1406579.1"
                     /translation="MFQQGDLIVYGNTGVCRVEAVGHPKGISGIPEDKLYYTLAPLYS
                     AGLIYAPLDTPVLMRPVLTREAAEKLIAQIPSIREDDCGSRDPKFLSEHYRGFFESCS
                     CEDLLQLIKTLYSKTQNSISNGRKPGQVDQRFMKRAEELLHGELAVALDIPAEEVADY
                     ISSQVGEEGAFSSLS"
     gene            1028..1723
                     /locus_tag="ACZPFK_09450"
     CDS             1028..1723
                     /locus_tag="ACZPFK_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1406580.1"
                     /translation="MKRKIIKIDEEKCNGCGLCAAACHEGAIGMVEGKARLLREDYCD
                     GLGDCLPACPTGAISFEEREAPAYDHEAVMAAKAQSAAPLPCGCPGSESKPLRRAPAA
                     APAGTDAPSELAQWPVQIKLAPVNAPYFSGADLLIAADCTAYAYGAFHQRFIRGRVTL
                     IGCPKLDEGDYAEKLAAILAHNEIRSVTVVRMEVPCCGGLENAVKRALQASGKFLPWH
                     VVVISTDGRILSE"
     gene            1736..2251
                     /locus_tag="ACZPFK_09455"
     CDS             1736..2251
                     /locus_tag="ACZPFK_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406581.1"
                     /translation="MEINEKSCFALWDQLLGALVALARAAENEPPSPSAHRAVLDGLA
                     CVLACYDARRLEETLSAVRAEKDRLAPLCATCTSPCGRTAAYGLRDLLAEPDARLRAL
                     KLSLLFSLQGLARCALAAQTDGLPDEETLSFFCRGLFALGYLFGPERLRELAEEGGLL
                     ALRRLEAGGPA"
BASE COUNT          471 a          920 c          890 g          487 t
ORIGIN      
        1 aaggttagcc gggcatttta tgcgcagtct gcaccaagtt tttttcttgc ccccccgcct
       61 tatttgcacc agaccgccaa aaaatggtaa acttaaatcg gtgtaacata atatcgacgg
      121 gccgcatgcc gtttcatcgg ccgtcccgcc ggccaggagc gccaaaaaaa gtagtgcacc
      181 agccgcggct gcggccgcgc tcccgactgg atggttgttt ttttgtcaaa aagacagaca
      241 gggccgcgcc tggtgggtcg cggtcttgtt tgtgtgtttt catcgccgcc cgccgggcgg
      301 cccgttgccc cgtgggggca gaacaggagg catgcgatgt ttcagcaggg ggacctgatc
      361 gtttacggca atacgggggt ctgccgggtg gaggccgtgg ggcacccgaa aggcatctcc
      421 ggtatcccgg aggataagct ctactacaca ctggccccgc tctactcggc gggcctgatc
      481 tatgccccgc tggacacccc ggtgctgatg cgccctgtgc tcacccgcga ggccgcggaa
      541 aagctgatcg cccagatccc ctccatccgc gaggacgact gcggtagccg cgaccccaaa
      601 tttttatccg agcactatcg gggctttttt gagagctgca gctgtgagga cctgctgcag
      661 ctgatcaaga ccctctattc caaaacgcag aactcgatca gcaacggccg caagcccggc
      721 caggtggacc agcgctttat gaagcgcgcc gaagagctgc tgcacggaga gctggccgtg
      781 gcgctggaca ttccggcgga ggaagtggcc gattatatct caagccaggt gggggaagag
      841 ggcgcttttt cctctctctc ctgatctctg ccggggctgc ggcgacgatg ggcagacgcc
      901 ttacgcggcg tctgcctttt ttgttggaaa aacaacatac atcttttctg ccccatgcta
      961 tgctgatggc atcggagggg agtttcgccg tttgcggccc cgcggacaag aaaggatgga
     1021 tcgacatatg aagcgcaaga tcatcaaaat agacgaggaa aaatgcaacg gctgcggcct
     1081 gtgtgccgcc gcctgccacg agggcgcgat cggcatggtg gagggcaagg cccgcctgct
     1141 gcgggaggac tactgcgacg ggctgggcga ctgcctgccg gcctgtccca ccggggccat
     1201 ctcttttgag gagagggagg cgcccgccta cgaccacgag gcggtgatgg ctgccaaagc
     1261 gcagagcgcc gcccccctgc cctgcggctg ccccggcagc gagagcaaac ccctgcgccg
     1321 ggcgcccgcc gctgcgcccg cagggacgga cgcgcccagc gagttggccc agtggccggt
     1381 gcaaatcaag ctggccccgg tgaacgcgcc ctatttcagc ggtgctgacc tgctcattgc
     1441 agccgactgc accgcctacg cctacggtgc gttccaccag cgctttatcc gcggccgggt
     1501 gaccctaatc ggatgcccca agctggatga gggcgactat gcggaaaagc tggccgcgat
     1561 cctggctcat aatgagatcc gcagcgtcac ggtggtgcgc atggaggtgc cctgctgcgg
     1621 tgggctggaa aacgccgtca agcgcgctct ccaggcaagc ggcaaatttt tgccctggca
     1681 tgtggtcgtg atctccacgg acggccgcat cctgtcggaa taaggcgggc gcgccatgga
     1741 gataaatgaa aaaagctgtt tcgccctttg ggatcagctg ctcggcgcac tggtcgcgct
     1801 ggccagggcg gcggaaaacg agcccccctc cccctcggcc caccgggcgg tgctggacgg
     1861 gctcgcctgt gtccttgcct gttatgacgc ccggcgcctg gaagagacct tgagcgccgt
     1921 ccgggcggaa aaagaccggc tggcgcccct gtgcgccacc tgtacctccc cctgcggccg
     1981 caccgctgcg tacggcctcc gcgatctgct ggccgagccg gacgcgcgcc tgcgcgccct
     2041 caagctgtca ctcctcttca gtctgcaggg gctggcccgc tgcgccctcg ccgcccagac
     2101 ggacggcctg cccgacgaag aaaccctctc ctttttttgc cgcggcctct ttgcgctggg
     2161 gtatctcttc ggccctgagc ggctgcggga gctggcggag gagggagggc tgctggcgct
     2221 gcgccggctg gaggccggag ggcctgcgtg acggcggcct gcggacccgg ccccttgccc
     2281 gggtccccaa aaagttttgt gcggcgcgcc ctgtggaaag ttcttttccg cagggcgcgc
     2341 ttatttgccc cggcgctgga aatagtgtat agtgaaaaca ttcctctccc cgcgccccgc
     2401 ggggcaaacg ttccgccggc cgcacagagg gccttgatcc ggaacgcggc gtcgggtaaa
     2461 cgcctcccac agcggcggag tcacggccgc cgcgccctgc ccctgcgccg ccaacagaac
     2521 ggtaaaatcc gcccaggacg gaagcttcgc gccgcgcccg gcgcatcccc gccccgcggc
     2581 caacccgtcc ctttgcacag aaaggagcgc tttcatgatc ccctctgagc tggagcctct
     2641 cacccgctgc cgcctgttct caggcatccc cgcagaggag cttcccgccc tgctgcgccg
     2701 cctcggggcg gcccggaagc ggtacggcca agacgagctg ctgctcacgg cggcagaggc
     2761 cgacggca
//
LOCUS       DBMDYA010000386         2764 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8002_length_2764_cov_1.269996, whole genome shotgun sequence.
ACCESSION   DBMDYA010000386 DBMDYA010000000
VERSION     DBMDYA010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8002_length_2764_cov_1.269996"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(82..>329)
                     /locus_tag="ACZPFK_09460"
     CDS             complement(82..>329)
                     /locus_tag="ACZPFK_09460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR1406582.1"
                     /translation="DRVDPAIDETLIKYVEDRKGHDRRYGIDPQKIKDELGWTPETTF
                     EVGIEKTIDWYLANVEWMERVTSGAYMEYYAEMYQNR"
     assembly_gap    330..339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<340..1123)
                     /gene="rfbB"
                     /locus_tag="ACZPFK_09465"
     CDS             complement(<340..1123)
                     /gene="rfbB"
                     /locus_tag="ACZPFK_09465"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1406583.1"
                     /translation="MKTYLVTGGAGFIGSNFVLYMLKKYDDIRIVNLDKLTYAGNLEN
                     LKSVERDPRHIFVQADICDREAVSALFEQYDVDVVVNFAAESHVDRSIKNPEIFVETN
                     VGGTVNMLQCAKNAWAEGEGFKAGKKYLQVSTDEVYGSLGETGYFTESTPLCPHSPYS
                     ASKASADMFVMAFHDTYGLPVNITRCSNNYGPYQFPEKLIPLMINNAKTRKALPVYGD
                     GMNVRDWLYVEDHCKAIDMVAQAGRDGEVYNAGGHNERPNIFI"
     gene            complement(1251..2168)
                     /gene="rfbD"
                     /locus_tag="ACZPFK_09470"
     CDS             complement(1251..2168)
                     /gene="rfbD"
                     /locus_tag="ACZPFK_09470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR1406584.1"
                     /translation="MKILISGSKGQLGTELQRMLAAGQSELGPLPQEVRGAAVACYDV
                     DTLDITDLAAVRRVIAAERPDVVINCSAFTNVDGCERETDAAFRVNALGPRNLAMAAA
                     ESGAKLVHVSTDYVFSGAPNGGVPLDEAALPAPVSAYGTTKLLGEEYVRQFCPRSFIL
                     RTAWLYSYTGKNFVFTMMNAGRRTGALTVVNDQLGNPTNAVDLGHHILKLAVTEQYGL
                     YHCTGNGVCSWYDFACEIIRLSGTPAAVTPCTSDEYAAAHPEAARRPAWSALDNRMLR
                     CTVGDEMRDWREALECFFAHYGTVDTGTP"
     gene            complement(2210..>2764)
                     /locus_tag="ACZPFK_09475"
     CDS             complement(2210..>2764)
                     /locus_tag="ACZPFK_09475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
                     /protein_id="HIR1406585.1"
                     /translation="FYGNGFGDLLRAAVKNAEGGRATIFGYYVNDPERFGIAEFDESG
                     KVLSVEEKPAHPKSNYAITGLYFYDRRVVELAKQVRPSARGELEITSLNDLYLRDGTL
                     DLQRLGRGYAWLDTGTMDSLVDAADFVRMIEKRQSIKISAPEEIAYKNGWISREKLLE
                     SARRYGKSPYGEHLKRVAEGKILY"
BASE COUNT          455 a          879 c          847 g          573 t
ORIGIN      
        1 gccaaccgga acaaaaacgc gccccgaaaa ttttcggggc gcgttttgcg tctgggccgc
       61 ggacgcggcc atttttcact tttaacggtt ctgatacatc tcggcatagt attccatata
      121 agcgccgctg gtgacccgct ccatccattc cacgttggcc agataccagt cgatggtctt
      181 ctcgatgccc acctcaaagg tggtctccgg ggtccagccc agctcgtcct tgatcttctg
      241 cgggtcgatg ccgtagcggc ggtcatggcc cttgcgatcc tccacatact tgatgagggt
      301 ctcgtcgatg gccgggtcca cccggtcggn nnnnnnnnnc gatgaagatg ttgggccgct
      361 cgttgtgccc gcccgcgttg tagacctcgc cgtcccgccc ggcctgggcc accatgtcga
      421 tggccttgca atggtcctcc acatacagcc agtcccgcac gttcatgccg tcgccgtaca
      481 ccggcagggc cttgcgggtc tttgcgttgt tgatcatgag ggggatcagc ttttcgggga
      541 attgataggg cccgtagttg ttggagcagc gggtgatgtt caccggcagc ccgtaggtgt
      601 cgtgaaaggc catcacgaac atgtcggcgc tggccttgct ggcggagtag gggctgtgcg
      661 ggcagagggg ggtgctctcg gtgaaatagc cggtctcccc cagcgagccg taaacctcgt
      721 cggtggacac ctgcaggtat tttttgcccg ccttgaagcc ctcgccctcg gcccaggcgt
      781 ttttggcgca ctgcagcatg ttcacggtgc cgcccacatt ggtctccaca aagatctccg
      841 ggtttttgat gctgcggtcc acgtggctct cggccgcaaa gttgaccacc acatccacgt
      901 cgtactgctc gaacagggcg ctgacggcct cgcggtcgca gatatccgcc tgcacaaaga
      961 tgtggcgcgg gtcccgctcc actgatttga gattttccag attgcccgca taggtcagct
     1021 tgtccaggtt gacaatgcgg atgtcatcgt attttttcag catatagagc acaaagttgg
     1081 agccgataaa cccggcgccg ccggtgacga gataagtctt cattttactt cctccgcgtt
     1141 cgcgttatag tggggctgtt gacaagccaa gggccgcaaa aaacgaaagc aggcttttct
     1201 ggctcaacga gtatttttgt ggaatgtgcc gcaacaaagc acacatgttt tcaaggggtg
     1261 cctgtgtcaa ccgtcccata gtgtgcaaaa aagcattcca gcgcctcgcg ccagtcccgc
     1321 atctcatcgc ccaccgtgca gcgcagcatg cggttgtcca gcgccgacca ggcggggcgg
     1381 cgggccgcct cggggtgggc ggccgcgtac tcgtcgctgg tgcagggggt cacggcggcc
     1441 ggcgtgccgg acagccggat gatctcgcag gcaaagtcgt accagctgca cacgccgttg
     1501 cccgtgcagt ggtagaggcc gtactgctcg gtgacggcca gcttcaggat atggtggccc
     1561 aggtccacgg cattggtggg gttgcccagt tggtcgttca ccaccgtcag cgcgccggtc
     1621 cttctgcccg cgttcatcat cgtgaagacg aagttcttgc ccgtatagct gtacagccag
     1681 gccgtgcgca ggatgaagct gcgcgggcag aactggcgca catattcctc gcccagcagc
     1741 ttggtggtgc cgtaggcgga caccggcgcg ggcagcgccg cctcgtccag cggcaccccg
     1801 ccgttgggcg cgccgctgaa cacataatcg gtggagacgt gcaccagctt ggcgccgctc
     1861 tccgccgcgg ccatggccag attgcgcggg cccagcgcgt tcacccgaaa cgccgcgtcg
     1921 gtctcccgct cgcagccgtc cacattggtg aacgccgagc agttgatcac cacatccggc
     1981 cgctcggcgg cgatcacccg gcgcaccgcc gcgagatcgg tgatatccag cgtgtcgacg
     2041 tcatagcagg ccactgccgc gccgcgcacc tcttggggca gggggcccag ctcgctctgc
     2101 ccggccgcca gcatccgctg cagctcggtg ccgagctggc ccttggaacc gctgatgaga
     2161 attttcatat gcaacgtcct tttccggccc gttttttcgg gccctgttct caatagagga
     2221 tcttgccctc ggccacccgt ttcaggtgct cgccgtaggg cgacttgccg taccgccggg
     2281 cggactccag cagtttttcc ctgctgatcc agccgttttt ataggcgatc tcctcggggg
     2341 ccgaaatctt gatgctctgg cgcttttcga tcatacgcac aaaatccgcc gcgtccacca
     2401 ggctgtccat ggtgccggtg tccagccagg cgtagccgcg ccccagccgc tgcaggtcca
     2461 gcgtgccgtc gcgcaggtag aggtcgttga ggctggtgat ctccagctcg ccgcgggccg
     2521 agggccgcac ctgctttgcc agctccacca cccgtcggtc ataaaaatac aggccggtga
     2581 tggcgtagtt ggatttggga tgggcgggct tctcctccac cgagaggacc ttgccgctct
     2641 cgtcgaactc ggcgatgccg aaccgctccg ggtcgttgac gtagtagccg aagatggtgg
     2701 cgcggccgcc ctcggcgttt ttcaccgccg cgcgcagcag atcgccaaag ccgttgccgt
     2761 aaaa
//
LOCUS       DBMDYA010000387         2764 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8004_length_2764_cov_0.942170, whole genome shotgun sequence.
ACCESSION   DBMDYA010000387 DBMDYA010000000
VERSION     DBMDYA010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8004_length_2764_cov_0.942170"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(7..570)
                     /locus_tag="ACZPFK_09480"
     CDS             complement(7..570)
                     /locus_tag="ACZPFK_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406586.1"
                     /translation="MRGKEKLTALLLCCALLLCGCTVTVRISQSGVEIDRDPAEKDPS
                     GETSAAAPGALFGPEDEVFVWCEGRAAGTEEEYAEISLAEQERKELEQLLQPEQWTPV
                     QEKPERDFPLVLGVDVRNGEEGTRLYLCRGEEGEALILRKKLKDGEETGESALYFVPS
                     QTVKDTERFLTRLLETGDPARADESGA"
     gene            complement(572..>2764)
                     /locus_tag="ACZPFK_09485"
     CDS             complement(572..>2764)
                     /locus_tag="ACZPFK_09485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1406587.1"
                     /translation="GGYLRSRRRLLRASRSSDALTAKMNAARAAVGVRRRVPLLVCPL
                     VEGPVLVGLASPRVLVPREDYDDETLAMCLRHELIHYRRRDVGLKLLMRLACCLYWYH
                     PCVWLMASLAGQDAELACDEEVVRGGDRQFRAAYSDSILRVLEQQRRQPPACSAAFGA
                     RGKRRLRERFLHIFDGGKKRAAVLLTALLLCVTLPAATLVACRGEASGGDDPAADAGL
                     GEARPLLTAEELPPEALPESALYLAETYEAVCGGSPHPEKDPDLYDPSWIYLGYGLLR
                     REGLVDEWLEQKKQSYPNSNYVVFTLYESAVLAQPFFVDPPPIQPLPLSENGESFGSI
                     AEDYRMQTSRLRLERLTRTEDGCLSAEFARIAEDGGFLGRITYLFAQAGPEVQPDEIL
                     AGAFPPDKPLWQIKAVLPDGQDASPAKPTPDPTPRADPVPENRTVEISTAQALVEMAA
                     EVNESEYGCLGVTYLLTADIDLAEVRFEPIGLNEDPEKSYWGDEAGWSGFNGTFDGQG
                     HTIANLTVLRWEGETPEDKNCAGLFAMIGADGEVKNLKLTNARIEGQGDSGILVGICR
                     GKAENCSVQGSIFGRDATGGFAGYLKPGAQITDCSARAQVMGACHVGGFGGSISDSVL
                     TGCTAEGTLTAYNSNTQMYPFSAPFRVGGFAGYLWGSRMENCHSGMRLIVRDAASYLG
                     AFVGEAFQSSCSGCTADAAAAGNWQLIDVIDWEDGVGEKMKDYDVRLV"
BASE COUNT          475 a         1010 c          760 g          519 t
ORIGIN      
        1 tctcttttac gccccgcttt catccgcccg ggcgggatcc cctgtttcga gaagacgcgt
       61 caaaaagcgc tccgtgtcct tgaccgtttg ggatgggacg aaatagagcg ctgattcccc
      121 ggtctcctcc ccatctttca gctttttgcg gaggatcagc gcctccccct cttccccccg
      181 gcacagatac agccgtgtgc cctcctcgcc gttccggacg tccacaccca gtaccagcgg
      241 gaaatccctt tccggcttct cctgcaccgg ggtccattgc tccggctgca gcaattgttc
      301 cagctctttc cgctcctgct ccgccagaga aatttccgca tattcctcct ctgtccctgc
      361 cgcgcggccc tcgcaccaca caaagacctc atcctccggg ccgaacagcg ccccaggcgc
      421 cgcagccgag gtctcgccgc ttgggtcctt ttcagccggg tcccggtcga tctcaacgcc
      481 gctctgtgaa atgcggacgg tcacggtgca gccgcagaga agcagggcgc agcagagcaa
      541 aagcgccgtc aacttttctt ttcctctcat ttcagaccag ccttacatca taatctttca
      601 tcttctcccc cacgccgtcc tcccaatcaa tgacgtcgat gagctgccag ttgcccgccg
      661 ccgccgcgtc cgccgtgcag cccgagcagc tgctctggaa agcctccccg acaaaagccc
      721 ccagatagct ggcggcgtcc cgtacgatca gccgcatccc gctgtggcag ttttccatcc
      781 ggctgcccca caaatatccc gcgaacccgc cgacccgaaa cggcgcggaa aaaggataca
      841 tctgcgtatt gctgttgtaa gcggtcagcg tgccctccgc cgtgcagccc gtcaaaacac
      901 tgtctgagat agagcctcca aagcctccta catggcaggc tcccatcacc tgagcccgcg
      961 cgctgcaatc ggttatttgc gcgcccggtt taagatagcc tgcgaagccc ccggtcgcat
     1021 ccctgccaaa aatgctgccc tgcaccgagc agttttccgc ctttcctctg cagatcccca
     1081 ccaggatccc cgagtcgccc tggccctcta ttctggcgtt tgtgagcttc aggtttttga
     1141 cctcgccgtc cgccccgatc atcgcaaaaa gccccgcaca atttttatcc tcgggggtct
     1201 ctccctccca gcgcaaaacg gtcaggttgg cgattgtgtg gccctgcccg tcaaaggtgc
     1261 cgttgaaccc cgaccacccc gcttcatcgc cccaataact tttctccggg tcctcgttca
     1321 ggccgatcgg ctcaaacctg acttccgcca ggtcgatatc cgccgtcagc aggtaggtca
     1381 cccccagaca gccatattcg ctctcattga cctctgccgc catctctaca agcgcctggg
     1441 cggtgctgat ctccacggtc ctgttctccg ggacggggtc ggcgcgtggg gtcgggtcgg
     1501 gcgttggctt tgctggggaa gcgtcctgcc cgtcgggcag gaccgccttg atctgccaga
     1561 gcggtttgtc cggcggaaaa gcgcccgcca aaatttcgtc gggctgcacc tccggcccgg
     1621 cctgtgcgaa aagataggtg atcctgccca aaaagccgcc atcctctgcg atgcgcgcaa
     1681 actctgccga caggcagccg tcctccgtgc gggtcaagcg ctcaaggcgc agccggcttg
     1741 tctgcattcg ataatcctcg gcgatcgacc cgaagctttc gccgttctcg ctcaacggca
     1801 gcggctgtat ggggggcgga tccacaaaaa acggctgggc aagcaccgcg ctttcataca
     1861 gtgtaaaaac gacataattg ctatttggat aagactgttt tttttgctcc agccattcgt
     1921 ccaccagccc ttccctgcgc agcaagccgt atcccagata gatccaggag gggtcgtaaa
     1981 ggtcagggtc cttttccgga tgaggggagc cgccgcacac cgcctcatag gtctccgcca
     2041 gatacaaagc gctttcgggc agcgcttccg gcggcagttc ctccgccgtc aacaggggcc
     2101 gggcctcccc tagccctgcg tcggccgccg ggtcatcccc tccgctcgcc tcgccccggc
     2161 aggccaccag ggtggcggcg ggcagcgtca cgcagagcag cagcgccgtc aacagcaccg
     2221 ccgcgcgctt tttgcccccg tcgaagatgt gcaaaaaccg ctcgcgcagc cgccgcttgc
     2281 cgcgcgcgcc gaacgctgcg ctgcaggcgg ggggctgccg gcgctgctgc tccagcacgc
     2341 gcaggatgct gtcgctgtag gcggcgcgga actgtctgtc cccgccccgc accacctcct
     2401 cgtcacaggc cagctcggcg tcctgcccgg ccagagaggc catcagccag acgcaggggt
     2461 gataccagta caggcagcag gccagccgca tcagcagctt gaggcccacg tcccgccgcc
     2521 ggtaatggat cagctcgtgg cgcaggcaca tcgccagcgt ctcgtcgtca tagtcttctc
     2581 ggggcaccag cacccgcggg gacgccaacc ccaccagcac cgggccctcc accagcgggc
     2641 agaccagcag cggcaccctg cggcgcacgc ctacagccgc gcgggccgca ttcatctttg
     2701 cggtcagcgc gtcgctggaa cgggacgccc gcagcaggcg gcggcgggag cgcagatacc
     2761 cgcc
//
LOCUS       DBMDYA010000388         2761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8012_length_2761_cov_1.939474, whole genome shotgun sequence.
ACCESSION   DBMDYA010000388 DBMDYA010000000
VERSION     DBMDYA010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8012_length_2761_cov_1.939474"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="ACZPFK_09490"
     CDS             complement(<1..188)
                     /locus_tag="ACZPFK_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406588.1"
                     /translation="MARRSSTRPRQSASAELFFDCAAIKKYPVEAAAMLQCIAVFVKM
                     GVTKNSGEDGRGSAQGGRR"
     gene            289..1062
                     /locus_tag="ACZPFK_09495"
     CDS             289..1062
                     /locus_tag="ACZPFK_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1406589.1"
                     /translation="MEHPQVLIDRAGCIGCGLCSRACVAHNIKMIDKKANAVLSSCLL
                     CGQCQAVCPQKAISMAGYEDDPIEKKGEPRLDPDQVLEVIRFRRSVRRFQPTPIPQEV
                     IGQILEAGRLTHTAKNRQDVSFAVLDQEKDRIEKMAVKLFRAVRPVAALFSPMAKNRE
                     IDDHFFFFSAPLALVILAEDKTNGLLAAQNMEFVAEAHGLGVLYSGFFTAAANASPKI
                     KKAIGVPKGKRVAMTLVLGYPDIRFLRSTPHKKLDVRSL"
     gene            1081..1449
                     /locus_tag="ACZPFK_09500"
     CDS             1081..1449
                     /locus_tag="ACZPFK_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1406590.1"
                     /translation="MEKGCEKNLKRIVAGFRQCRNAFTAIGDETRQLILLTLLESELS
                     GIRVGEIAQKTHLTRPSVSHHLKILKEAGIVAVRRAGTKNYYYLSEDETRWREIADLV
                     NTVYASIRHISAPSAEEGTT"
     gene            1446..2198
                     /locus_tag="ACZPFK_09505"
     CDS             1446..2198
                     /locus_tag="ACZPFK_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394413.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HIR1406591.1"
                     /translation="MILYFSGTGNSEYAAKRIGKEIGDEVLNLFEKIKNSDVSALHSA
                     RPWVVVAPTYAWRIPRILHKWLQSTPLTGNKDLYFVMTCGGSIGNAAAHLEKLCAEKG
                     MRYRGCAGIRMPENYIALFSTPTGEEAEPILRQAEKSIDSTALLIRRGDKLPQADITL
                     ADKANSGIVNDIFYPLFVHAKKFYVTDDCVSCKKCADVCPLGNIKLADGKPVWGDRCT
                     HCMACISRCPKEAIEYGTHSRGLPRYLCPKQA"
     gene            complement(2423..2686)
                     /locus_tag="ACZPFK_09510"
     CDS             complement(2423..2686)
                     /locus_tag="ACZPFK_09510"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR1406592.1"
                     /translation="MKELTLFTMRGCPHCRMAREYMRELCQEDPRYARVPVNEIDENE
                     NAALADSYDYYYVPCYFMGDKKLHEGACTKEKIRKVLDTALNA"
BASE COUNT          605 a          833 c          775 g          548 t
ORIGIN      
        1 cgcctcccgc cctgcgcact tccgcggccg tcttctcctg aatttttagt aacccccatt
       61 ttaacaaaaa cagcaatgca ttgcaacatc gcggcagctt caacaggata tttttttatg
      121 gctgcgcaat caaaaaacaa ttcagctgaa gcgctctgcc ggggacgggt ggaggagcgc
      181 cgtgccacta tttctgtctg ttttggtaac ggctttcttt accgatgttg ttttggcccc
      241 agccgcttga tacaatggtt aaagaacatg aacgaaagga gccaaaaaat ggaacatccg
      301 caggtgctga tcgaccgggc cggctgtatc ggatgcgggc tgtgcagccg ggcctgcgtc
      361 gcgcacaata taaaaatgat cgataaaaag gcgaatgcgg tcctgagcag ctgcctcctc
      421 tgcgggcaat gccaggctgt ctgcccccaa aaagcgattt cgatggccgg gtatgaagac
      481 gatccgatcg agaaaaaagg ggagccccgg ctcgaccccg atcaggtact ggaggtgatc
      541 cgcttccgca gaagtgtccg ccgatttcag ccgaccccta tcccgcaaga ggtgatcggg
      601 caaatcctgg aggccgggcg gctcacccac acggcaaaaa accggcagga cgtatccttt
      661 gccgtgctgg atcaagaaaa ggaccgcatc gaaaaaatgg cggtcaagct gttcagggcc
      721 gtgcggcccg ttgccgccct gttcagcccc atggcgaaaa accgcgagat cgacgaccac
      781 tttttctttt tcagcgcccc ccttgccctt gtgatcctgg cagaggacaa gacaaacggg
      841 ctgctcgccg cccaaaatat ggaattcgtg gcggaggccc acgggctggg cgttttatac
      901 agcggttttt tcacagcggc cgccaacgcc tcaccaaaaa tcaaaaaagc gatcggcgtg
      961 cccaaaggca aaagggtcgc gatgactctg gtgctgggct acccggatat ccgctttctg
     1021 cgctcgaccc ctcataaaaa gctggacgtc cgatctctat agcgcatagg aggcttttgg
     1081 atggaaaaag gatgcgaaaa aaatttaaag cggatcgtcg ccggtttccg gcagtgccgc
     1141 aacgctttca ccgccatcgg cgacgagacg cggcagctca ttctgctcac gctgctggaa
     1201 agcgagctgt ccggcatccg tgtgggcgag atcgcgcaaa agacccatct cacacggccc
     1261 tcggtctccc atcatctcaa aatattgaaa gaggcgggca ttgtcgccgt gcggcgcgcg
     1321 ggcacgaaaa attattacta tctaagcgag gacgagacgc ggtggagaga gatcgccgac
     1381 ctggtcaata ccgtctatgc cagcatccgg catatcagcg cgccgtccgc agaggaggga
     1441 acaacatgat tttatacttt tcaggcaccg gcaacagtga atatgctgcc aagaggatcg
     1501 gcaaggaaat cggggacgag gtcctgaacc tttttgaaaa gatcaaaaac agcgatgttt
     1561 cagcgcttca ctcggcgcgc ccctgggtcg tcgttgcgcc gacctatgcc tggcgcatcc
     1621 cccgcatcct gcacaagtgg ctgcaaagca cccctctcac cggaaacaaa gacctgtact
     1681 ttgtgatgac ctgcggcggg agcatcggga acgccgccgc ccatctcgaa aagctctgcg
     1741 ctgaaaaggg gatgcgatat cgcggctgtg cgggcatccg catgcccgaa aattacatcg
     1801 cgctgttctc caccccgacc ggggaggagg cggagccgat cctccggcag gcggaaaaat
     1861 ccatcgacag caccgcgctt ttgatccggc gcggcgacaa gctgccccaa gcggatatta
     1921 ccctcgccga caaggcaaac agcggcattg tgaacgacat tttttatccc ctgttcgttc
     1981 acgcaaaaaa gttttatgtc accgacgact gcgtttcctg taaaaaatgt gccgacgtct
     2041 gtcccctggg caatatcaag ctcgcggacg gaaaaccggt gtggggggac cgctgcaccc
     2101 actgcatggc ttgcatcagc cgctgcccca aggaagcgat cgaatatgga acgcacagca
     2161 ggggcctgcc gcgctatctc tgcccgaaac aggcctagca aaaccggcgg gccaaggccg
     2221 gggtcttccg gcccccgacg cgccttggcc ctctacgggc acaaaagtcc gccgccaagc
     2281 gggcgggttt tgcactccct gcgggcgccc ataaaaaaac agagagcggg ccgccggggg
     2341 cggctctgct ctctgtttct gtctgtgccc atgcgccgtg gcgacgcttt ttctcacggc
     2401 gcggcgtccg tccccccgcg cgtcaggcgt tcagcgcggt gtccagcacc ttgcggatct
     2461 tttctttggt acaggcgccc tcgtgcagct ttttgtcccc cataaaatag cagggcacat
     2521 aataatagtc gtagctgtcg gccagggccg cgttctcgtt ttcatcgatc tcgttcaccg
     2581 gcacccgggc atagcgcggg tcctcctggc agagctcccg catatattcc cgggccatgc
     2641 ggcaatgggg acagcctcgc atggtgaaca gggtcagttc tttcattctc ttcgcctccc
     2701 tcagtggacg gtccgccccg cgcggctcta tcgtgccggg ccgcacgggt cttcttttat
     2761 a
//
LOCUS       DBMDYA010000389         2760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8020_length_2760_cov_1.251974, whole genome shotgun sequence.
ACCESSION   DBMDYA010000389 DBMDYA010000000
VERSION     DBMDYA010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_2760_cov_1.251974"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="ACZPFK_09515"
     CDS             <1..457
                     /locus_tag="ACZPFK_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406593.1"
                     /translation="FLPVLLLSGWLTFHYAAVYGAAAVSWTAFFATSTLWLLPTLLLV
                     TALSMLVTVATGTPAAVALQALWWFQELMRGVGGLSDGSTYGSLLTPRHNSLGQYFAF
                     QQHLPRLLGGRLLAVALAALLAAATVFVFDSKRKGGFSWRGRFASAARS"
     gene            421..1038
                     /locus_tag="ACZPFK_09520"
     CDS             421..1038
                     /locus_tag="ACZPFK_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406594.1"
                     /translation="MARALRICRTQLTHLCLLPLLGAVAFWLLIPVLFGTAMLDARAA
                     SQLLEGVFPLAGALLLVPLFAPERRPGLLDCVAARRASHLWVCGLRAAAMLGVLALLD
                     AGLWLLMAAQGCAVSAAQLLAAFANGLALGALGLLASALSASEIVGALAALGWYTMDF
                     FSDSCGGASLFLLSRGRGAPKLFVLAAGLLLLGAAFLVFYRRMRR"
     gene            1314..2192
                     /locus_tag="ACZPFK_09525"
     CDS             1314..2192
                     /locus_tag="ACZPFK_09525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1406595.1"
                     /translation="MVFSMTGYGRAEQVVHGRDITVEVKSVNARYFEYSSRVPRAYTW
                     VDDRLKKRLSAAISRGKTELSLTVVDVGAAGTVISVNEELARGYLTALRSLSASLGIE
                     DDVTTGTLTRFSDIFTVARAEMDEEQVFADIAAVADEAIAAFVAMRGAEGEKLAEDVL
                     ARLEAVERMVGEVEQLAEGRVQAYTDRLYEKLKALLQDRTVDDARVLTEAAIFADKTA
                     VDEETVRLRSHLKQYREILASGGPVGRKLDFLTQELNRETNTIGSKTQDLAVTRLVVE
                     MKAEIEKIREQIQNIE"
     gene            2208..2477
                     /gene="remA"
                     /locus_tag="ACZPFK_09530"
     CDS             2208..2477
                     /gene="remA"
                     /locus_tag="ACZPFK_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm regulator RemA"
                     /protein_id="HIR1406596.1"
                     /translation="MKLINIGFGNMVSANRLIAIVSPESAPIKRIIQEARDRGSLIDA
                     TYGRRTRAVIITDSDHVVLSAVQPETVANRLNDADDIEDTELEDE"
     gene            2470..>2760
                     /locus_tag="ACZPFK_09535"
     CDS             2470..>2760
                     /locus_tag="ACZPFK_09535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1406597.1"
                     /translation="MSNGQHLIVLSGPAGSGKDTVVRELIEKHPEIEVSVSVTTRDKR
                     PGEREGVNYYYISSEEFERRIAAGEVLEYTNYCGNYYGTPKSEVDRRIAAGID"
BASE COUNT          430 a          854 c          939 g          537 t
ORIGIN      
        1 gtttttgccg gtgctgctgc tgagcggctg gctgactttc cactatgccg cggtctatgg
       61 cgccgccgcg gtcagctgga cggctttctt cgcgacctcc accctctggc tgctgccgac
      121 gctgctgctc gtgaccgccc tgtccatgct ggtcacggtg gccaccggca cccccgccgc
      181 ggtggcgctg caggcgctgt ggtggtttca agagctgatg cgtggtgtcg gcggcctctc
      241 ggacggcagc acctatggct ccctgttgac gccgcgccac aattcgctgg ggcagtattt
      301 cgccttccag cagcatctgc cccggctgct gggcggccgg ctgctggccg tggcgctggc
      361 ggcgctgctg gcggcggcga ccgtttttgt gttcgacagc aagagaaagg ggggcttttc
      421 atggcgcggg cgcttcgcat ctgccgcacg cagctgactc atctgtgtct gctgccgctg
      481 ctgggggccg tggctttttg gctgctgatc ccggttctct tcggcaccgc gatgctggac
      541 gcgcgggccg cctcccagct gttggagggc gtctttccgc tggcgggggc gctgcttctg
      601 gtgccgctgt ttgcgccgga gcgccgcccc ggcctgctgg attgcgtcgc cgcccggcgc
      661 gcctcccacc tgtgggtctg cgggctgcgg gccgccgcga tgctgggtgt actggcgctg
      721 ctggacgccg ggctctggct gctcatggcc gcgcagggat gtgccgtctc ggcggctcag
      781 ctgcttgcgg cgtttgccaa cgggctggcg ctgggggcgc tggggctttt ggcctcagcc
      841 ctgtccgcca gcgagatcgt gggcgcgcta gcggctctgg gctggtatac gatggacttt
      901 ttctccgaca gctgcggggg cgcttccctg tttttgctgt cccgcgggcg gggcgcgccc
      961 aagctttttg ttttggcggc ggggctgctg ctgctcgggg cggctttctt ggtcttttac
     1021 cggcgcatgc ggcggtaaaa aggaggggcg gccgaaagcg ctgccgccct ccttaccgct
     1081 tttctggggc cggtggccgc gcctttcccc ttttgaggga atgcgcggcc accggccctt
     1141 tcgcatcctg ttcggggcgc ttgtaaaaaa gcttttgttt tctccgcttc tctgctgttt
     1201 tctcctgctt ttacctcctt ttgaattgac aaaccaccgg tgttcagtgt attattatta
     1261 cctgtatagc ccaaaacaat aagtgcccgg cagggggcaa aagagggtgc gacatggttt
     1321 tcagcatgac cggctacggc cgggcggagc aggtggtgca cgggcgggat atcacggtgg
     1381 aggtcaaaag cgtcaatgcg cgctattttg agtacagctc ccgggtgccc cgcgcctata
     1441 cctgggtgga cgaccggctc aaaaaaaggc tttccgccgc catctcccgc ggcaagaccg
     1501 agctgtcgct cacggtggtg gacgtggggg cggcgggcac cgtcatcagc gtcaacgaag
     1561 agctggcccg cgggtacctc accgccctgc gcagcctctc ggcctccctg gggatagagg
     1621 acgatgtcac caccggcacg ctgacccgct tttcggatat cttcacggtg gcccgggccg
     1681 agatggatga agaacaggtc tttgccgata tcgccgccgt ggccgacgag gcgatcgcgg
     1741 cctttgtggc gatgcgcggc gccgaggggg aaaagctggc cgaggatgtg ctggcccgcc
     1801 ttgaggctgt ggagcgcatg gtgggcgagg tggaacagct ggcggagggg agggtgcagg
     1861 cctacaccga ccggctgtac gaaaagctga aagcgctgct gcaggaccgc accgtcgacg
     1921 acgcgcgggt gctcaccgag gccgccatct ttgccgacaa gaccgccgtg gacgaggaga
     1981 cggtgcggct gcgcagccat ctcaagcagt atcgggaaat tttggcctcc ggcggcccgg
     2041 tgggtcgcaa gctggacttt ttgacccagg agctcaaccg ggagaccaac accatcggct
     2101 cgaaaacgca ggatctggcc gtcacccgcc tggtggtgga gatgaaagcc gagatcgaaa
     2161 agatccgcga gcagatccag aatatcgaat agaaaggggt aaccgccatg aaactgatca
     2221 acatcggctt tggcaacatg gtctcggcca accgtctgat cgccatcgtc agcccggagt
     2281 ccgcccccat caagcggatc atccaggagg cgcgcgaccg cggcagcctg atcgacgcca
     2341 cctacggccg ccgcacccgc gcggtcatca tcaccgactc cgaccatgtg gtgctcagcg
     2401 ccgtgcagcc ggagacggtg gccaaccgcc tgaacgacgc cgacgacatc gaggatacgg
     2461 agctggaaga tgagtaacgg acaacatttg atcgtgcttt cgggccctgc cggtagcggc
     2521 aaggacaccg ttgtgcgcga gctgatcgaa aaacaccccg agatcgaggt gtcggtatcg
     2581 gtgaccaccc gcgacaaacg ccccggcgag cgggaggggg tcaactatta ttacatcagc
     2641 agcgaggagt ttgagcgccg tatcgcggcc ggggaggtgc tggagtacac caactactgc
     2701 ggcaattact acggcacccc caagtcggag gtggaccgcc gcatcgcggc cggcatcgac
//
LOCUS       DBMDYA010000390         2746 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8071_length_2746_cov_1.593951, whole genome shotgun sequence.
ACCESSION   DBMDYA010000390 DBMDYA010000000
VERSION     DBMDYA010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8071_length_2746_cov_1.593951"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(246..776)
                     /locus_tag="ACZPFK_09540"
     CDS             complement(246..776)
                     /locus_tag="ACZPFK_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR1406598.1"
                     /translation="MDHKELWKKCVAFHGHECGGLTIGYQAALYAAELLDLTFSSDEQ
                     VVCISENDACGVDAIQVMLGCSVGKGNLLFHMTGKQAFSFYDRRTGRSVRLVLKRKPR
                     EMSREESFQYYQSCSPDEMFDVKEAKISIPEHARLFDSYVCECCGETTGTNWIRLQGG
                     KKLCVDCYVQYDRFHV"
     gene            complement(1026..2162)
                     /locus_tag="ACZPFK_09545"
     CDS             complement(1026..2162)
                     /locus_tag="ACZPFK_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HIR1406599.1"
                     /translation="MKKTVVLILTAALALSLCACGGSSSAPAGGASSSAARGGSSASA
                     SGYEVTDLMGRKVTIPAGASRYACIGPGALRLYCYVADDAQLAGVEEIERSSQDGRPY
                     AMSIPGVAELPLIGPGGPANGPDAELLFAAGPDVIFTCYTAELSTVDELQAKTGIPVV
                     ALRYGTGRLFDQSVYDSLSLIGRITGNATRADEVIDYFEEVKSDLTARTADVSERPSA
                     YLGCQSSRGSHGIESTTGDYAIFDVLNAVNVVKEAGISEYVMLDKEKLLEMDPEVIVI
                     DAGGLSLLQEDYAANPQFYKTLSAFKNGRVYMQMPFNYYTTNLEIALADAYYIGSVLY
                     PEQFADLDPAEKFDELSSFFLGIDCYEQIAGQYYGGFQPVVLGG"
     gene            complement(2189..>2746)
                     /locus_tag="ACZPFK_09550"
     CDS             complement(2189..>2746)
                     /locus_tag="ACZPFK_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406600.1"
                     /translation="ITHMDGATLAKHIGYVSQSCEFAESTVFDAVLLGRKPFIKWDVT
                     GRDLEIVQEVLDLLSLESYALRSVNELSGGERQKVAIARVLAQQTPVLLFDEPTSNLD
                     LKNQLEVLRIIKKIVREKELTAVVTIHDLNLALRFADRFLIMKEGRVYADGGAETVNA
                     DSIREVYGVAASVAEFGSHRVVIPE"
BASE COUNT          528 a          874 c          794 g          550 t
ORIGIN      
        1 ttcggttgcc gcgacagcgg cgagaagcgg ggccattgaa ataagttgtt ttttattgcg
       61 cagcaataaa aaatagccgc aggctatttt ttcggccatc cgtttcggtt gccgcggcag
      121 cggcgagaaa cggggccatt gagatcagtt atttttttca aaataatcaa taaaaaattt
      181 tcgctttctc ctgaagcgcc ggtcttgtgc aatcgtcccc tcagcgtctg cccaaaggcg
      241 ctttctcaca catgaaagcg gtcgtactgc acatagcagt cgacacacag ctttttgccg
      301 ccctgcagcc tgatccagtt ggtccccgtc gtctcgccgc agcactcgca gacatacgag
      361 tcaaagaggc gggcgtgctc tggaatgctg atctttgctt ctttcacatc aaacatctcg
      421 tcggggctgc agctctggta atattggaac gactcctcac ggctcatctc ccggggtttg
      481 cgcttgagta ccaggcgcac ggaccgcccg gtcctgcggt cataaaacga aaaggcctgt
      541 ttgcccgtca tgtggaacag caggttgccc tttcccacac tgcaccccag cattacctgg
      601 atggcgtcca cgccgcaggc gtcgttttca gagatgcaga cgacctgctc gtcgctggaa
      661 aaggtgaggt ccagcagctc ggccgcatac agcgccgcct ggtaaccgat ggtcaggccg
      721 ccgcactcgt gcccgtgaaa ggcgacgcat ttcttccaaa gctctttgtg atccattttt
      781 tcattctcct ttttgcgata tgggcccgcc gcgaatgggg ccgcgcggaa agtgcaaaca
      841 gcgcggacgg cgcgggtagc ctttccggcg ccggggccgg aacacaccgg caagcactta
      901 cagccttgca agcccccggt aagtccccat atcccggcgc ccggtaagcc cttgaagccg
      961 ggccgggacc ggggcgccgc gactttcaag gcgcgggttt cccgctgttt ttaccgtatc
     1021 cgtgttcagc cgcccagaac gaccggctga aaaccgccgt aatactgccc ggcgatctgc
     1081 tcatagcagt cgatgccaag aaagaagctg gacaattcgt caaacttttc ggcggggtcc
     1141 aggtcggcaa actgctcggg atacagcaca ctgccgatat aataggcgtc cgccagggcg
     1201 atctccaggt tggtggtata gtagttgaac ggcatctgca tgtaaaccct gccgttttta
     1261 aaggcgctga gggtcttgta gaactgcggg tttgcggcgt agtcctcctg cagcagcgag
     1321 aggccgcccg catcgatgac gatcacttcc gggtccatct ccagcagctt ctccttgtcc
     1381 agcatcacgt attcgctgat gcccgcctcc ttgaccacgt tgacggcgtt gaggacgtca
     1441 aagatcgcgt agtcgccggt ggtgctctcg atgccgtgcg agccgcggct ggactggcag
     1501 ccgagatacg ccgagggcct ttcagagacg tcggccgtcc gcgcggtcaa gtcgcttttg
     1561 acctcttcaa agtaatcgat cacctcatcg gcgcgcgttg cattgcccgt gatccggccg
     1621 atcagggaca gcgagtcata taccgactgg tcgaaaagcc gccccgtgcc gtagcggagg
     1681 gcgacgacgg ggatacccgt cttggcctgc agctcatcga cagtggacag ctcggctgta
     1741 tagcaggtga agatgacatc cggcccagcc gcgaacagca gctccgcgtc gggcccattt
     1801 gcggggccgc ccgggccgat gaggggcagc tccgccacgc cggggatcga catggcgtag
     1861 ggccgcccat cctggctcga ccgctcgatc tcctccacgc cggcaagctg cgcatcgtcg
     1921 gcgacatagc agtaaagccg cagcgcgccg gggccgatgc aggcgtagcg ggaggccccc
     1981 gccgggatgg tcaccttgcg ccccatcagg tcggtgacct cgtacccaga agcagacgca
     2041 gacgaaccgc cccgggcggc cgaggacgag gcgccgcccg caggtgcgga cgacgagccg
     2101 ccgcaggcgc acaggctcag cgccagcgcg gcggtcaaga tcagtacaac tgtctttttc
     2161 atgaaaaacg ctccctttct ctcttcttct attcgggtat cacgacccga tggctgccaa
     2221 actccgccac cgaggccgcc acgccgtaca cctcacggat gctgtcggcg ttcaccgtct
     2281 ctgcgccccc gtcggcgtag acacggccct ctttcatgat gaggaaccgg tccgcaaagc
     2341 gcagcgcaag attcaggtcg tggatggtga ccacagcggt cagctctttt tcccgcacga
     2401 tctttttgat gatgcgcagc acctccagtt ggtttttcaa gtccagattg ctggtcggct
     2461 cgtcaaagag aagcaccggg gtctgctggg ccagcacccg cgcgatggcc accttttgcc
     2521 gctcgccgcc ggacagctcg ttcacgctgc gcagggcata gctctccagc gacaagaggt
     2581 ccagcacctc ctgcacgatc tccaggtccc ggcccgtcac atcccatttg ataaacggct
     2641 ttcgccccag cagcacagcg tcaaacaccg tgctctcagc aaactcgcag ctctgggaca
     2701 cataaccgat gtgctttgcc agcgtggcgc cgtccatgtg ggtgat
//
LOCUS       DBMDYA010000391         2734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8110_length_2734_cov_1.375997, whole genome shotgun sequence.
ACCESSION   DBMDYA010000391 DBMDYA010000000
VERSION     DBMDYA010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_2734_cov_1.375997"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..669
                     /locus_tag="ACZPFK_09555"
     CDS             121..669
                     /locus_tag="ACZPFK_09555"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1406601.1"
                     /translation="MGTLNIVLVEPQIPQNTGNISRTCAVTGARLHLVGPMGFTITDK
                     QLKRAGLDYWDLLDVRLYDGLPEFFEKNAGPFYYFTTKGRRRYSDVAYPDGAYLVFGR
                     EDRGLPEPLLFANEACCVRLPMLANPHARSLNLSNTVAIAAYEALRQWDFDGLKEAGE
                     LTAFDWADAPGVQERKGGSAEG"
     gene            666..1547
                     /locus_tag="ACZPFK_09560"
     CDS             666..1547
                     /locus_tag="ACZPFK_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1406602.1"
                     /translation="MKKYAVMTDSTCDIPQELAQKHGIDILSFTIMLDGKEYSEREDL
                     TPDRFYELLDAAQGMPTTAQITPMTFEEKFAAYDAAGVEELLYVAINSGGSSTYNNAL
                     FAAAEYGDAHPDSRMRISIVDSHTYSMGYGRFVIRAAEKLAAGAEMADVVDYLKARFA
                     QVEIVLSVHSLKIIKRSGRISAAAAFAGELMGLRPVITLIQGETRVVQKVRGDAKMLP
                     ALTAYMKAHAGERSPYMVGATKGASDRHGDARQLSRLCEEAMGYPPECIFRLGAAVTS
                     NTGTDAVALVYLTGDES"
     gene            1890..2168
                     /locus_tag="ACZPFK_09565"
     CDS             1890..2168
                     /locus_tag="ACZPFK_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIR1406603.1"
                     /translation="MSLQQTFKALGDPTRREILQRLGKGRLTAGQLGEGLGLTGATIS
                     HHLSCLKEAGLVNDTREGKYIYYELDTSVMEDIFRWAASLTGGDGHEK"
     gene            2241..>2734
                     /locus_tag="ACZPFK_09570"
     CDS             2241..>2734
                     /locus_tag="ACZPFK_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019468.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIR1406604.1"
                     /translation="MKKNRSELLCWLLAALPLALALFCLPTLPQRVPLHWDLAGEVDD
                     WGSPASALLIPAMTLGINALFALLPRIDPKRRNYQRFGGAYTAFRLVFNLFMLGMLCV
                     TLYSAYRPGALPVNKVIPAGVGALFCVMGNYMPKFKPNYFLGLRTPWTLSSERVWHST
                     HRLAG"
BASE COUNT          481 a          856 c          953 g          444 t
ORIGIN      
        1 ggccctgcgc tttaccgaga acgccgcccg cgccaccttt gccgcgcggc gcgacccgcg
       61 ctttgggctg gagtttgagc cgctgctggg cgagctgctg ccgccccggg aggaaccgtc
      121 atgggcacgc tgaacatcgt gctggtggag ccgcagatcc ctcagaacac cggcaacatc
      181 tcccgcacct gcgcggtgac cggggcccgg ctgcacctgg tgggcccgat ggggttcacc
      241 atcaccgata agcagctcaa gcgggcgggc ctggattatt gggatctgct ggacgtgcgc
      301 ctttacgacg ggctgccgga attttttgag aaaaacgccg gcccgtttta ctatttcacc
      361 accaaggggc gccgccgcta cagcgacgtg gcctacccgg acggggccta tctggtgttt
      421 ggccgggagg acaggggcct gcccgagccg ctgctgttcg ccaacgaggc gtgctgtgtg
      481 cggctgccga tgctggccaa tccgcacgcc cgcagtctga acctgtcgaa tacggtcgcc
      541 atcgcggcct atgaggcgct gcggcagtgg gattttgacg gcctgaaaga agcgggggag
      601 ctgaccgcct ttgactgggc ggacgccccc ggcgtgcagg agaggaaagg aggctcggcc
      661 gagggatgaa aaaatacgcg gtgatgaccg attccacctg cgatatcccg caggagctgg
      721 cgcaaaagca cggcatcgac atcctcagct ttaccatcat gttggacggc aaagagtatt
      781 ccgagcggga ggatctgacc cccgaccgct tttacgagct gctggacgcg gcccagggca
      841 tgcccaccac ggcccagatc accccgatga cctttgaaga gaaattcgcc gcctacgacg
      901 cggccggggt ggaggagctg ctctatgtgg cgatcaactc cggcggcagc agcacctaca
      961 acaacgcgct gttcgccgcg gccgaatatg gggacgcgca cccagacagc cggatgcgca
     1021 tctccatcgt ggacagccat acttactcga tgggctacgg ccgctttgtc atccgggcgg
     1081 cggaaaagct ggccgcgggc gccgagatgg cggatgtggt ggactatctg aaagcgcggt
     1141 tcgcccaggt ggagatcgtg ctctcggtgc acagcctcaa gatcatcaag cgcagcgggc
     1201 gcatctccgc ggcggcggcg tttgccggtg agctgatggg cctgcggccc gtcatcaccc
     1261 tgatacaggg cgagacccgg gtggtgcaga aggtgcgcgg cgacgccaag atgctgccgg
     1321 ccctgaccgc ctatatgaaa gcccacgccg gcgagcgcag cccctatatg gtgggggcca
     1381 ccaagggcgc gtcggaccgg cacggggacg cgcgccagct gtcgcggctc tgcgaggagg
     1441 ccatgggcta tccacccgag tgcatcttcc ggctgggggc ggcggtgacc agcaacaccg
     1501 gcaccgacgc cgtcgccctg gtctatctga cgggggacga aagctgagcg ccgggcggca
     1561 gagaaaaaag aatgacaagg cagaaaatgc ggccgcggcg gccgcatttt tttatgctcc
     1621 cgccccgggg ccgtcgggcg ggagcgcgcg gaagaaaaca ggagggagcg ggaggggggt
     1681 aggaaagagc gggagcgcgc agggggacgc agagagcccc gggggaagcg cgggacgatc
     1741 gccatagtct caggcccgcg cgcccggccc gggttcggca gctccccggc agggcgaccc
     1801 tgccccggcg cccctttccg ccttgccctt tttttcggtt tgcagtacaa taaacttaga
     1861 aagctgtcta aatgaaaggg ggcgggcgga tgtcgctgca gcagacgttc aaggccttgg
     1921 gcgaccccac gcggcgcgag attctgcagc ggcttggcaa ggggcggctc acggcggggc
     1981 agttgggcga gggccttggg ctcaccggcg ccaccatcag ccaccacctg tcctgtctga
     2041 aagaggccgg gctggtgaac gacacccgcg agggcaaata catctactac gagctggaca
     2101 cctcggtcat ggaggatatt ttccgctggg cggcgtcgct gacgggaggg gacggacatg
     2161 aaaaatagcg ccgtccgcgc cttgcggccg gcgcgggccg caggggcgaa tctggtttca
     2221 ggcagagggg ggaggacggc atgaaaaaga accgaagcga gctgctctgc tggctgctgg
     2281 cggcgctgcc gctggcgctg gccctttttt gcctgcccac cctgccgcag cgggtgcccc
     2341 tgcactggga tctggccggg gaggtggacg actggggctc gcccgcgtcg gccctgctga
     2401 tcccggccat gacgctgggc atcaacgcgc tgttcgcgct gctgccccgc atcgacccca
     2461 aacggcgcaa ttatcagcgc ttcggcggcg cttacaccgc gttccggctg gtgttcaacc
     2521 tgtttatgct gggcatgctg tgcgtcaccc tctacagcgc ctatcggccc ggcgcattgc
     2581 cggtgaacaa ggtgatcccg gccggggtgg gcgcactctt ctgtgtgatg ggcaattaca
     2641 tgcccaagtt caagccgaat tattttttgg gcctgcgcac cccctggacg ctgtccagcg
     2701 agcgggtgtg gcacagcacc caccgcctgg ccgg
//
LOCUS       DBMDYA010000392         2730 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8128_length_2730_cov_1.355649, whole genome shotgun sequence.
ACCESSION   DBMDYA010000392 DBMDYA010000000
VERSION     DBMDYA010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8128_length_2730_cov_1.355649"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2730)
                     /gene="mfd"
                     /locus_tag="ACZPFK_09575"
     CDS             complement(<1..>2730)
                     /gene="mfd"
                     /locus_tag="ACZPFK_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1406605.1"
                     /translation="VGGRCNLLAAPLEGALQLTMPRADFEANTLTLREGQTFKQQDLV
                     ARLVSAGYHRRDKVEGPGQFSVRGGIVDLYPPDMDRPARIEFWGDVVDTMSGFDLLTQ
                     RREVQLKKVYVSPAREVLFSDPAEAAALLRDAAARLKGAKKARLESVMAEDLAALDAG
                     TLPEHMDKYLALRYAQPMTALDYFENPLFFFEEPSAVREAAWALAFRTGEELKGLLEQ
                     QVLTAELAHFYEDYDALLLRADKAPAVVCENFSRTLPDFQPKELVSVTAHALPAWGGE
                     VKSLVEDLQNYKAAGYFCAVLAGTRRAADALARDLCAAGLTAVSAKGDPVPTAGAVAV
                     LPGHLSAGTDYPFAKFALITSRRQKVEEQKPRRKKAKGLSSLEDLKPGDYVVHQNHGI
                     GVYTSIERLDLQGVVKDYIKIQYAGADTLFVPVTQLDLISRYTAPGDGEKVKLAKLGG
                     GDWAKTKTRVKKATEEMAKDLLALYARRKTAKGFAFPSDGDWQRDFETRFEYEETDDQ
                     LTSAAEIKKDMERPWPMDRLLCGDVGVGKTEVALRAAFKAIMGGKQVAILVPTTILAW
                     QHFNTILQRMEAYPVKAAMLSRFRTPKQIKDALRGLRDGTVDIVVGTHRLLQKDVHFK
                     DLGLIIVDEEQRFGVKHKEKLKENFPGVDVLTLSATPIPRTLNMAMSGIRDMSTIEQP
                     PFERQPVETFVLEYDEGVLEQAITRELARGGQVYYLYNRVETIEACAVRVAKMAPGAR
                     IATAHGKMTEEQLSTVWQALLDGNIDVLVCTTIIETGVDVRNCNTLIIEDADRMGLAQ
                     LYQIRGRVGRSGRKAYAYFTFRPAKVLTDVAAKRLSAIREFTAFGSGFRIAMRDLQIR
                     GAGNLLGQSQHGHMDAVGYDMYVKLLNQAVASLKGEAAPPDKSDCLIDVTVDA"
BASE COUNT          412 a          902 c          914 g          502 t
ORIGIN      
        1 aggcgtccac ggtcacgtcg atcaggcaat cgctcttgtc cggtggggcg gcctcgccct
       61 tgagggaggc cacggcctga ttcagcagct tgacgtacat gtcgtagccc accgcgtcca
      121 tgtggccgtg ctggctctgc cccagcaggt tgcccgcgcc gcggatctgc agatcgcgca
      181 tggcgatgcg gaagccgctg ccgaaagcgg tgaactcgcg gatggcggac agccgctttg
      241 ccgccacgtc ggtgagtacc ttggccgggc ggaatgtgaa gtaagcgtag gccttgcggc
      301 cgctgcggcc cacccggccg cggatctggt agagctgggc cagccccatg cggtcggcgt
      361 cctcgatgat gagggtgttg cagttgcgca cgtccacgcc ggtctcgatg atggtagtgc
      421 agaccagcac gtcgatgttg ccgtccagca gcgcctgcca gacggtggag agctgctcct
      481 cggtcatctt gccgtgggcg gtggcaatgc gtgcgcccgg cgccatcttg gccaccctca
      541 ccgcacaggc ctcgatggtc tccacgcggt tgtacaggta ataaacctgg ccgccccggg
      601 ccagctcgcg ggtgatggcc tgctccagca cgccctcgtc gtattccagc acaaaggtct
      661 ccaccggctg gcgctcaaag gggggctgct caatggtgct catgtcccgg atgccgctca
      721 tggccatatt cagggtgcgg gggatggggg tggccgagag ggtcagcacg tccacgccgg
      781 ggaaattttc tttcagcttt tccttgtgct tgacgccgaa gcgctgctcc tcgtccacga
      841 tgatgaggcc caggtccttg aagtggacgt ccttttgcag cagccggtgg gtgcccacca
      901 cgatatccac cgtgccgtcc cgcaggcccc gcagggcgtc cttgatctgt ttcggggtgc
      961 ggaagcggga gagcatcgcc gccttgacgg ggtaggcctc catgcgctgc aaaatggtgt
     1021 tgaaatgctg ccaggcaagg atggtggtgg gtaccaggat ggcgacctgc ttgccgccca
     1081 tgatggcctt gaacgccgcg cgcagcgcca cctcggtctt gcccaccccc acgtcgccgc
     1141 agagcagccg gtccatgggc caggggcgct ccatgtcctt tttgatctcg gcggcggagg
     1201 tcagctggtc gtcggtctcc tcgtattcaa agcgggtctc aaagtcccgc tgccagtccc
     1261 cgtccgaggg gaaagcgaag ccctttgccg tcttgcggcg ggcatagagc gccagcaggt
     1321 ccttggccat ctcctcggtg gcctttttga ccctggtttt ggtcttggcc cagtcgccgc
     1381 cgcccagctt tgccagcttc accttttcgc catcccccgg cgcggtgtag cggctgatca
     1441 ggtccagctg ggtgaccggc acgaacaggg tgtccgcgcc cgcgtattgg atcttgatgt
     1501 agtccttgac gaccccctgc aggtccagcc gctcgatgga ggtgtagacg ccgatgccgt
     1561 ggttttgatg caccacgtaa tccccgggct tcagatcctc caggctggag aggcctttcg
     1621 cctttttgcg gcgcggcttc tgctcctcca ccttttgacg gcgggaggtg atgagggcga
     1681 attttgcaaa cgggtaatcg gtgcccgccg acagatgccc gggcagcacc gccaccgcgc
     1741 ccgccgtggg caccgggtcg cccttggcgg acaccgcggt cagcccggcg gcgcagaggt
     1801 cccgcgccag ggcgtcggcg gcgcgccggg tgccggccag cacggcgcaa aaatagccgg
     1861 cggctttgta gttttgcagg tcctccacca gcgacttcac ctcgcccccc caggcgggca
     1921 gggcgtgggc ggtgacgctg accagctctt tgggctgaaa atccggcagg gtgcgggaga
     1981 aattttcgca caccacggca ggggccttgt ccgcccgcag cagcagcgcg tcgtaatcct
     2041 cgtagaaatg cgccagctcg gcggtgagca cctgctgctc cagcaggcct ttcagctcct
     2101 ccccggtgcg gaacgccagg gcccacgcgg cctcccgcac ggcgctgggc tcctcaaaaa
     2161 agaagagcgg attctcgaag tagtcgaggg cggtcatcgg ctgtgcatac cgcagggcca
     2221 gatatttgtc catgtgctcg ggcagggtgc cggcgtccag ggcggccagg tcctccgcca
     2281 tcacgctttc cagccgggcc tttttcgccc ccttgaggcg ggcggcggcg tcgcgcagca
     2341 gggcggccgc ctcggctggg tcagagaaca gcacctccct ggccggggag acgtagacct
     2401 ttttcagctg cacttcacgg cgctgggtca gcaggtcaaa gccggacatg gtgtccacca
     2461 cgtcccccca gaactcgatg cgggccgggc ggtccatgtc gggcggatag agatcgacga
     2521 tgccgccgcg cacgctgaac tggcccgggc cctccacctt gtcccgccgg tgatatccgg
     2581 ccgagaccag ccgggccacc aggtcctgct gcttgaaagt ctggccctcc cgcagggtga
     2641 gggtgttggc ctcaaagtcg gcccggggca tggtgagctg caaagcgccc tccagcgggg
     2701 cggccaacag gttgcagcgg ccccccacga
//
LOCUS       DBMDYA010000393         2727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8140_length_2727_cov_1.485910, whole genome shotgun sequence.
ACCESSION   DBMDYA010000393 DBMDYA010000000
VERSION     DBMDYA010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8140_length_2727_cov_1.485910"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="ACZPFK_09580"
     CDS             <1..1180
                     /locus_tag="ACZPFK_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015563.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1406606.1"
                     /translation="QGFIDVADIIFFIVFAYGFVYMLIKNGTFDALIGSMLRGLGNKV
                     GALIPLAMVLFIFLGSTVGILEETYGMIPIFIGLAVAMGYDAIVGSAMVYVGAATGFA
                     AATLNPFTIGVAQTVAGVEIGSGIGYRILCAVIFGGIAIGYTMWYAHRVKKDPTKSLM
                     YGEKMELASSTDRDKLQATYATGRQKACGVLFVLTIAGLLVGTLKFGWYINQLAGLFL
                     IMMIVVGLVGGFGPSKIAETFVESTKGVIFGALVVGLSRGVLIVMNEAQIVDTIVNFL
                     ANLLARTSGYLSAVGMLVMQNIINFFIPSGSGQAAVTMPIMAPVSDMIGLSRQIAVLA
                     FQFGDGFSNMFWPTTVCMMCGIMNIPLNKWYKFIAPMFAMMFLAQILLMCGAVAIGYV
                     "
     gene            1313..2515
                     /locus_tag="ACZPFK_09585"
     CDS             1313..2515
                     /locus_tag="ACZPFK_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1406607.1"
                     /translation="MDCEQLKARVFALTDEMQPLLEEVCDAVFHHPELGLAEAWSSDY
                     LVKKIEAQGFAVQKPYCGLETAFRAEKGSGGPKIAFLAEYDALPGYGPGKDQLGHACG
                     HNWIAASSFGAAAVLGRLTEELGCTAVWIGTPAEETVGGKVDLVRAGAFDDVDLVLQM
                     HLGDRTELHPHFLAMDSIEFTFEGVATHASNTPERGVNALDACNLTFCGINALRQHIT
                     PDARIHGIITQGGVAPNITPSHCVARFYVRAARREYLNSLTEKVINCARGAELMTGAK
                     MSYRYFENPFDDLNNCAPLLPRMEANLRAAGVEEFRDPEPNSAGSSDIGNVSHVCPTL
                     YIPMETGNTDGSCCHDEAFLAHVNGPDAYRALNRGVKGMAATAVDALCDEALWQAIRA
                     WVKRDGRQ"
BASE COUNT          475 a          887 c          864 g          501 t
ORIGIN      
        1 gcagggcttc atcgacgtgg ccgacatcat ctttttcatc gtcttcgcct acggctttgt
       61 ctatatgctg atcaaaaacg gcacctttga tgcgctgatc ggctcgatgc tgcgcggcct
      121 gggcaacaag gtgggggcgc tgatcccgct ggcgatggtg ctgttcatct ttttgggctc
      181 cacggtgggc atcctggagg agacctacgg catgatcccg atcttcatcg gcctggcggt
      241 cgccatgggc tacgacgcca tcgtgggcag cgccatggtc tatgtgggcg cggccaccgg
      301 ctttgccgcc gccaccctca acccgttcac catcggcgtt gcccagacgg tggcgggggt
      361 ggagatcggc tccggcatcg gctaccgcat cctctgcgcg gtcatcttcg gcggcatcgc
      421 catcggctac accatgtggt acgcccaccg ggtcaaaaag gaccccacca agagcctgat
      481 gtacggcgaa aagatggagc tcgcctcctc caccgaccgc gataagctgc aggcgaccta
      541 cgccaccggc cgccagaaag cctgcggtgt gctgttcgtg ctcaccatcg ccgggctgct
      601 ggtgggcacc ctcaagttcg gctggtacat caaccagctg gcgggcctgt ttttgatcat
      661 gatgatcgtg gtgggcctgg tgggcggctt tggccccagc aaaatcgccg agacctttgt
      721 ggaatccacc aagggcgtca tcttcggcgc gctggtggtg ggcctctccc gcggggtgct
      781 gatcgtgatg aacgaggcgc agatcgtgga caccatcgtc aactttctgg ccaacctgct
      841 ggcccgcacc agcggctacc tgagcgcggt gggcatgctg gtgatgcaga acatcatcaa
      901 cttcttcatc ccctccggca gcggccaggc ggcggtcacc atgcccatca tggccccggt
      961 gtccgacatg atcggcctgt cccgccagat cgcggtgctg gcgttccagt tcggcgacgg
     1021 cttctccaac atgttctggc ccaccaccgt ctgcatgatg tgcggcatca tgaacatccc
     1081 gctcaacaag tggtacaagt tcatcgcgcc gatgttcgcc atgatgttcc tggcgcagat
     1141 tctgctgatg tgcggcgcgg tggccatcgg gtatgtataa ggcgtccctt tctgccgcat
     1201 gcgtcccgcc ctttgcggcg gagcggaatc cacatattga taaaaagcgt tcctccccgt
     1261 gcccgccgct ctgccgccgg gccggacgga ggcattgaaa ggatggttcc ccatggattg
     1321 tgagcagctc aaagcgcggg tcttcgcgct gacagacgag atgcagcccc tgttggaaga
     1381 ggtctgcgac gccgtgttcc accacccgga gctgggcctc gccgaggcct ggtcgtccga
     1441 ttacctggtc aaaaagatcg aggcccaggg ctttgcggtg caaaagccct attgcgggct
     1501 ggagacggcc tttcgggctg aaaagggcag cggcggcccc aagatcgcct ttctggcgga
     1561 atacgacgcc ctgcccggct acggccccgg caaggatcag ctgggccacg cctgcggcca
     1621 caactggatc gccgcctcct cctttggggc cgcggcggtg ctggggcgcc tgacggagga
     1681 gctgggctgc acggccgtct ggatcggcac cccggccgag gagacggtgg gcggcaaggt
     1741 ggacctggtg cgcgcgggtg cctttgacga cgtggacctg gtgctgcaga tgcatctggg
     1801 cgatcgcacc gagctgcacc cccacttttt ggccatggac tccatcgagt tcactttcga
     1861 gggggtggcg acccatgcct ccaacacgcc ggagcgcggg gtcaacgcgc tggacgcctg
     1921 caacctgact ttctgcggca tcaacgcgct gcgccagcac atcacgcccg acgcccgcat
     1981 ccacggcatc atcacccagg gcggcgtggc ccccaacatc acccccagcc actgtgtggc
     2041 ccgcttttat gtgcgcgcgg cgcggcggga gtatctgaac agcctcaccg aaaaggtcat
     2101 caactgcgcc cgcggggctg agctgatgac cggcgcgaag atgagttacc gctattttga
     2161 aaacccgttc gacgacctga acaactgcgc cccgctgctg ccgcggatgg aggcgaacct
     2221 gcgggccgcc ggggtggagg agttccgcga cccggagccc aacagcgccg ggtccagcga
     2281 catcggcaac gtctcccatg tctgccccac cctttatatc cccatggaga ccggcaacac
     2341 cgacggctcc tgctgccacg acgaggcctt tttggcccac gtcaacgggc cggacgccta
     2401 ccgggccctg aaccggggcg tcaagggcat ggccgccacc gcagtggacg cgctctgtga
     2461 cgaggcgcta tggcaggcca tccgggcctg ggtcaaaagg gacgggcggc aataaaagaa
     2521 gccaagacaa aacagccgcc ttagacgcat tgagacaaga aatgttggtt ctgggccgac
     2581 ggccaggacg tccggcagat cgtgacgctc agcagattta aaaaaggttg gcaatccatt
     2641 cgggttgcca accttttttg cacggcgcct tttgtcaaag ccgccggtcc gggcccgccg
     2701 cgcgccccgc gggcggggaa agccgcg
//
LOCUS       DBMDYA010000394         2725 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8148_length_2725_cov_1.459985, whole genome shotgun sequence.
ACCESSION   DBMDYA010000394 DBMDYA010000000
VERSION     DBMDYA010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8148_length_2725_cov_1.459985"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="ACZPFK_09590"
     CDS             complement(<1..777)
                     /locus_tag="ACZPFK_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406608.1"
                     /translation="MTKLARYLKPYILPILLCVVLLFVQANADLNLPNYMSDIVNVGI
                     QQSGVEHSYPEAISADGLALMKTFLSEEDRALADRAYTLVEEGVPEWEKLKKEYPAIT
                     GAVYTAGRLDAQDSAALDTAFGRAGYALVLLMQDLAGGAGQAAQEAGDSASFDLTQLY
                     PMLPMLAAQQPAVDKAVSAAAAAPEQTTGSTGALFAKSFLAELGADTAAIQSRYVMKT
                     GGKMLLVSLVGAVSSILVGLLASRVGAGAARTLRGDVFSKV"
     gene            complement(892..1440)
                     /locus_tag="ACZPFK_09595"
     CDS             complement(892..1440)
                     /locus_tag="ACZPFK_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013231.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1406609.1"
                     /translation="MSTSEEIDQRTRELFATAAEIKDLFFNYSPLPELSHGQFLMLRH
                     IYQFENDQCLFAAEEEPPGGEGGLKITALAKLMRNAPPTVSQKADDLERLGYLQRRRG
                     RRDRRAVYVSLTPQGQALMARAMRLYDHLGRRVVARLGDQQIRVFVETLRNLKEALAA
                     EQAEFAAALARGEEGQNAESNN"
     gene            complement(2294..2557)
                     /locus_tag="ACZPFK_09600"
     CDS             complement(2294..2557)
                     /locus_tag="ACZPFK_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406610.1"
                     /translation="MALLALLAAGAALLCPYVIWALGMLFRQTADPGTGTTFQAWPLC
                     AGLSGPLMRLNLRFPALYSLVGAALWALGFPRAQGRAAATTKQ"
BASE COUNT          495 a          899 c          888 g          443 t
ORIGIN      
        1 gacttttgag aacacgtccc cgcgcagggt gcgggccgcg cccgcgccca cccgggaggc
       61 cagcaggccg accaggatgc tggacaccgc gccgaccagc gagaccagca gcatcttgcc
      121 gccggtcttc atcacatagc gggactgaat agccgccgtg tccgcgccca gttctgccaa
      181 aaagcttttg gcgaaaagcg ccccggtgga gccggtggtc tgctccggcg cggcggccgc
      241 ggcggacacc gccttgtcca ccgcgggctg ctgtgccgcc agcatgggca gcatcgggta
      301 gagctgggtc aggtcaaagg aggcgctgtc gcccgcctcc tgcgccgcct gcccggcccc
      361 gcccgccagg tcctgcatca gcagcaccag ggcgtagccg gcgcggccga aagccgtgtc
      421 gagggccgcg ctgtcctgcg cgtccagccg cccggcggtg tagacggcgc cggtgatggc
      481 cgggtactcc tttttcagct tctcccactc ggggacgccc tcctccacca gcgtgtaggc
      541 gcggtcagcg agcgcgcgat cctcctcgct caaaaaggtc ttcatcagcg ccaggccgtc
      601 cgcgctgatc gcctccgggt agctgtgctc cacgccgctc tgctggatgc ccacgttgac
      661 gatgtcggac atgtagttgg gcaggttcag gtcggcgttg gcctgcacga agagcagcac
      721 cacgcacagc aggatgggca ggatgtaggg cttgagatat cttgccagct ttgtcatctg
      781 cgttcctctc ctctctcagg ttctgaatgg gcccccaagg gccgccgcgg cgcgggggac
      841 cgcccgcctg tgcggccgca gccggcaggg caggcgcggc gcgccgggcg ctcagttgtt
      901 cgattcggca ttttgcccct cctccccgcg cgccagggcg gccgcaaact cggcctgctc
      961 cgccgcaagg gcttctttta aatttctcag cgtctcgacg aagacgcgga tctgctggtc
     1021 ccccagccgg gccaccaccc ggcggcccag atggtcgtac agccgcatgg cgcgcgccat
     1081 cagcgcctgg ccctgggggg tgagcgagac atagaccgcc cgccggtcgc gccggccgcg
     1141 ccgccgctgc aaatagccca gccgctcaag gtcgtcggct ttctggctca cggtgggggg
     1201 cgcgttgcgc atgagcttcg ccagcgccgt gatcttgagc cccccctcgc cgccgggcgg
     1261 ctcctcctcc gccgcgaaaa ggcattggtc gttttcaaac tgatagatgt ggcgcagcat
     1321 caaaaactgc ccgtggctca actccggcag gggcgagtaa ttgaagaaca ggtccttgat
     1381 ctccgccgcg gtggcaaaca gctcccgcgt gcgctggtcg atctcctccg acgtggacac
     1441 gcccgaacca cctcctgtgc atagattggc cgccgccggg gcccggtgcg cacggccggt
     1501 gcgccacccg cgaaagcggt cggcaaagat tcgcaaggcg aatatttagg caggctaaac
     1561 aatttgcgaa agtggttcta tcatatgccc tttgcactaa aaaatcaatg aattttttgt
     1621 taaaagtgcg aaacaaaata gagggataag ccctttttca gaagagctcc ggggccggga
     1681 gacggagagg acaagcggac gcgccccgtt tgcacccacg gccgggagct tgtggacagg
     1741 gagagggaga tacgggtgcg cccccgcacc cggtattcat tttccgcacc acaaaaagcg
     1801 ccccgccgtt tatcggcagg gcgcgcttat ctaaaattat gggagaggcc cgggcggttt
     1861 ccagctggag gggcgcaagg tcccggggat acgtcttctg tcttctcggg caagaggaaa
     1921 cggctttcgc cccgcgggag acagagaggc aggcgggcgg gggatataac tgcggaaata
     1981 agaaataccg acagctcggt gggggcgttt taagataggc gcccgcgccg gagaaccgcg
     2041 gtttttttat ggagcagcaa taaaatttat cctgacggcc ccgtttcccg tcgctatcac
     2101 agcggccgcc ccacggcctt gaaatctcaa gcgcccaccc acggagggga cggatgcgcg
     2161 caaaaaacag cttattttga tggccccgtt tctcgccgct gcgcggcagc cgaaacggac
     2221 gctccaaaaa gcgacttgtt tctatggcca gggcccgggg acgggccgca tagccgcaaa
     2281 caatagcccg cggctattgt ttcgttgtgg ccgccgctct gccctgcgcg cggggaaagc
     2341 ccagcgccca aagggccgcg ccgaccagcg aatagagcgc cgggaagcgg agattgagtc
     2401 gcatcagcgg gccggagagc ccggcacaga gcggccaggc ctgaaaagtc gtgcccgtgc
     2461 cggggtccgc cgtttgacgg aaaagcatcc ccagggccca gatcacatag gggcagagca
     2521 aggcggcccc ggcggccagc agggccaaca gcgccacccg gaccggtttt acctagggcc
     2581 ggtctttcag gggctttgcc ccgaacagca gctccattgc ctgcagggcg caccagccca
     2641 aaagcagcag cgccgccgga tccagcagca ggctcaccag atcggccggg atcgtcagat
     2701 agcgggatgc cgccacctgg gcggc
//
LOCUS       DBMDYA010000395         2724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8156_length_2724_cov_1.199771, whole genome shotgun sequence.
ACCESSION   DBMDYA010000395 DBMDYA010000000
VERSION     DBMDYA010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8156_length_2724_cov_1.199771"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            387..1124
                     /locus_tag="ACZPFK_09605"
     CDS             387..1124
                     /locus_tag="ACZPFK_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1406611.1"
                     /translation="MDKKNKFSSMWKGKGFYIALGVCVFAAVISSALAINNMMGKLEA
                     QNPAGSAAASESGVNDPWEQGEEVGKTESKVPVLPGSSSQSQSAASSGSTSGAAQGAQ
                     QQPADGQPVQEGSFSLPVDGKVSAAYSGDELVYNNTLADWRTHNGIDIAAAAGAQVKA
                     AMAGTVAKVAEDDMWGNVVEVTSGDLTLRYCGLAEDVAVKQGDSVSLGQQLGALSEVP
                     AEVAEEPHLHLEVLRGEATINPEEIMK"
     gene            1632..2030
                     /locus_tag="ACZPFK_09610"
                     /pseudo
     CDS             1632..2030
                     /locus_tag="ACZPFK_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2232..>2724
                     /locus_tag="ACZPFK_09615"
     CDS             2232..>2724
                     /locus_tag="ACZPFK_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024256.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1406612.1"
                     /translation="MYAGNYDFWYESSRLMQQLIRNQNKKKEEKIKELQSFIQRFSAN
                     KSKSKQATSRKKVLESIQLEEMPASSRKYPFVGFSMERELGKDILFVEGLSKTVDGVK
                     VLDNVSFSVGRGEKIALIGDNEAGQTALFQILAGELEPDAGSFKWGVSTSRSYFPKDN
                     SAFF"
BASE COUNT          551 a          804 c          924 g          445 t
ORIGIN      
        1 ggcgcgaccg agagataacg catcggattc atctggcaac acctctttcc ggcgtttgcc
       61 ggggataggg ggcgaagaaa cgggaaagct gagagagaaa ggcttcctcg actattctag
      121 ccaatcccgg cgcaaaagac aagcgggcgg caggggcggg gccgaccgcc gggggcgaag
      181 cgcccgcgcg ctttcccgcc cggggcctgc ggagcgattt tcccccgcgg gctttgcggc
      241 ggccgtgcat aaaccggggc cggggggcca acaataggga taaaccgggg ccgggacggc
      301 ccggaaaacg gcgctcagcg cagaaaaaac agccgcgcgg cgggtggggg gacccatggc
      361 cgcttgtggc gcgaaaggaa cagaagatgg acaagaaaaa caaattttcc tcgatgtgga
      421 aaggcaaggg cttttacatc gcgctggggg tctgtgtctt tgctgcggtc atttccagcg
      481 cgctggccat caacaacatg atgggcaaac tggaggcgca aaatcccgcg ggcagcgccg
      541 cggcaagcga gagtggggtg aacgatccgt gggagcaggg agaagaagtc ggcaaaaccg
      601 agagcaaggt gcccgtattg ccgggctcgt catcacagtc gcagagtgcg gcgtcatcgg
      661 gttctacctc tggcgctgcg caaggggcgc agcagcagcc cgccgatggg cagcctgtgc
      721 aagagggatc gttcagcttg ccggtggacg gtaaggtgag cgccgcctac agcggcgacg
      781 agctggtcta caacaacacg ctcgccgatt ggcgcaccca caacggcatt gatatcgccg
      841 ccgccgcggg cgcccaggtc aaggcggcca tggccggcac ggtggccaag gtggccgagg
      901 acgatatgtg gggcaacgtg gtggaggtca ccagcggcga cctgacgctg cgctactgcg
      961 gccttgcaga ggacgtggcg gtcaagcagg gcgacagcgt ctcgctgggc cagcagctgg
     1021 gcgccctctc cgaggtgccc gcagaggtgg cggaggagcc gcacctgcat cttgaagtgc
     1081 tgcgcggcga ggccaccatc aacccggaag agatcatgaa ataggctgcg gcagcaccgc
     1141 aaaagccctg aaaggcgcga aaaacgcttt tcagggcttt ttggcgcccg ccgcccttgc
     1201 gcgccggggc ggttccatgg taaaatagcc gggcaacggt tttgacttcc cctttttccg
     1261 gcgctgcccc ggggccctga tgccccgtcc acgggacggg cgggcgcaaa aaagcggcgg
     1321 cggggcggcc ggtttttgat cgcccggttc aaaacagctg ctcttggggg ctttggttgc
     1381 cgccgctgcc gcgacggccg atgctcctct gcggcggcca agcggcctgc gggccaaaac
     1441 tcttgggccg ccgccctcag ggagggtcaa gcggccgcag gtgtggaccg gcgcccgccc
     1501 gcgcggggcc ggattttgat ttttctggag gaacggattt gttaacagtc agcgatgtga
     1561 gcctgcaatt tgacggggcc gacctgttca agcatgtgga tctgaaattt accgacggca
     1621 actgctacgg catcatcggc gccaacgggg cgggcaagag caccctgctg cgcattttgt
     1681 ccggcgagct ggagcccacc accggcagcg tgtccctgag ccccggcacc cgcatgagcg
     1741 tgctgcagca ggaccacttc aaatttgacg ggtacacggt gatggatacc gtcatcatgg
     1801 gcaaccgccg cctgtacgac gtgcgctgcg agaaagacgc cctctatatg aaagaggatt
     1861 tttcagaggc ggacggcgag cgggcggccg agctggaggg cgagttcgcc gagctgggcg
     1921 gctgggacgc ggagaccgag gcggctcagc tgctcaacgg ccttggcatc ccccccgccc
     1981 agcacgacgc gcagatgtcc agcctcgacg gccgcggcaa ggtgaaagtg ctgctggccc
     2041 aggccctgtt cgggcagccc aacatcgtgc tgctggacga gcccaccaac tatctggaca
     2101 tcgagtccat cgactggctg gaggagtttc tcatcgatta cccctatacg gtgctggtgg
     2161 tcagccacga ccgccatttt ctcaacgcgg tctgcaccca cacggtggat atcgactaca
     2221 accaggtgcg catgtatgcg ggcaactacg atttctggta cgaatcgtcc aggctgatgc
     2281 agcagctcat ccgcaaccag aacaagaaaa aagaggaaaa gatcaaagag ctgcagtcct
     2341 ttatccagcg cttttccgcc aacaaatcca agtcgaagca ggccaccagc cgcaaaaagg
     2401 tgctggagtc gatccagctg gaggagatgc ccgcctccag ccgcaaatac ccgtttgtgg
     2461 gcttttccat ggagcgggag ctgggcaagg atatcctttt cgtggagggc ctctccaaga
     2521 cggtggacgg ggtcaaggtg ctggacaacg tctctttcag cgtgggccgg ggcgagaaga
     2581 tcgccctgat cggggacaac gaggcggggc agacggccct gttccagatt ttggccggcg
     2641 agctggagcc ggacgcgggc agctttaagt ggggcgtatc caccagccgc agctatttcc
     2701 ccaaagacaa ttccgcgttt ttca
//
LOCUS       DBMDYA010000396         2721 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8166_length_2721_cov_1.366794, whole genome shotgun sequence.
ACCESSION   DBMDYA010000396 DBMDYA010000000
VERSION     DBMDYA010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8166_length_2721_cov_1.366794"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="sdaAA"
                     /locus_tag="ACZPFK_09620"
     CDS             complement(<1..705)
                     /gene="sdaAA"
                     /locus_tag="ACZPFK_09620"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1406613.1"
                     /translation="MYNMIEDLLEEQRRTGEPLHRIILKNEMELSELSEEEIYSGLKK
                     RYEVMAASAGKALQQPQKMVLGLIEGQASRQRQYVDGQSICGPFLNELMAMALSCSEV
                     NASMGRICAAPTAGSCGILPAVLLAVSRRYGSSEKEVLSALLVAGGFGAVIVKNSTVS
                     GAEGGCQAECGAAAAMAAAAAVTLAGGTAEMAANACGIALMNVMGLVCDPVAGLVQLP
                     CSYRNASGAVNAVLSAD"
     gene            complement(726..1388)
                     /gene="sdaAB"
                     /locus_tag="ACZPFK_09625"
     CDS             complement(726..1388)
                     /gene="sdaAB"
                     /locus_tag="ACZPFK_09625"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR1406614.1"
                     /translation="MNISLFEVLGPVMIGPSSSHTAGAAKLARVARIIAEHPFHRVTF
                     GLYGSFAKTGVGHGTRQALLAGAMGFQEDDERIKNIEPLAAEAGMEYEFYDVELDDAH
                     ENTAVITFYRDDGEKTQVTGCSVGGGRIKITDIDGLAANIRAEAPTLLITQNDKPGVV
                     GAVSGLLAEGGLNIAVMRVSRVAKGDIANCVIETDGRIGDELVRRIRQLPNVRSVRAI
                     NI"
     gene            complement(1661..2245)
                     /locus_tag="ACZPFK_09630"
     CDS             complement(1661..2245)
                     /locus_tag="ACZPFK_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015553.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HIR1406615.1"
                     /translation="MAKTLFSAVAAFVATNLDDLFLLMLLFGQARDGGGRRRVVAGQY
                     LGMGALTTLSLLGAWGAWLLPRRAVALLGLAPLALGIKAWRDGRKDAGDGADDAAGRG
                     LSVFAVALLTVANGGDNIGVYLPVFSGYSAAQMGLAAAAFAALTGLWCAAGAGLAALP
                     RVKGPLHRCQRVLVPLVLVGLGLFILLGGLLQAG"
     gene            2540..>2721
                     /locus_tag="ACZPFK_09635"
     CDS             2540..>2721
                     /locus_tag="ACZPFK_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406616.1"
                     /translation="METATYTKGILIEQTECDLKNRMTLGALLRRAQQAATGHCDAVG
                     VTTAYLLGYHAAFLLV"
BASE COUNT          433 a         1014 c          829 g          445 t
ORIGIN      
        1 atcggcggaa agtaccgcgt tgaccgcgcc cgaggcgttg cggtagctgc agggcagctg
       61 caccagcccc gccaccgggt cgcagacaag gcccatcacg ttcatgaggg cgatgccgca
      121 ggcgttggcc gccatctcgg cggtgccccc ggccagggtg accgcggccg ccgccgccat
      181 cgccgcagcc gcgccgcact ctgcctggca gccgccctcg gcgccggaca cggtggaatt
      241 tttgacgatg accgcgccga agccccccgc caccagcagg gccgagagga cctccttttc
      301 gctggagccg taccgccgcg acaccgccag cagaaccgcc ggcaggatgc cgcagctgcc
      361 cgccgtcggg gcagcacaga tgcgccccat cgaggcgttc acctcgctgc aggacagggc
      421 catagccatc agctcattga gaaagggccc gcagatgctc tggccgtcca catactgccg
      481 ctgccggctg gcctgcccct caatgaggcc cagcaccatc ttctgcggct gctgcagcgc
      541 cttgcccgcg ctggcggcca tcacctcgta tctctttttc aggccgctgt agatctcctc
      601 ctcactgagc tcggacagct ccatctcatt tttcaggatg atgcggtgca gcggctcgcc
      661 ggtgcggcgc tgctcctcca gcaggtcctc gatcatattg tacatcgtct ctccccctcc
      721 cctgttcaaa tattgatggc gcgcacgctg cgcacattgg gcagctgccg gatgcgccgc
      781 accagctcgt cgccgatgcg cccgtccgtt tcgatgacac agttggcgat atcccctttt
      841 gccacccgcg acacccgcat gacggcgata ttgaggccgc cctccgccag caggccgctg
      901 accgcgccca ccacgccggg cttgtcgttc tgggtgatga gcagggtggg cgcctcggcg
      961 cggatgttgg ccgccagacc gtcgatgtcg gtgatcttga tccgcccgcc gcccaccgaa
     1021 cagccggtca cctgtgtctt ttcgccgtcg tcgcggtaaa aggtgatcac cgccgtgttc
     1081 tcgtgggcgt cgtccagctc cacgtcgtaa aactcgtatt ccatgcccgc ctcggccgcc
     1141 agcggctcga tgtttttgat gcgctcgtcg tcctcttgaa agcccatcgc cccggccagc
     1201 agcgcctggc gggtgccgtg ccccacgccg gtctttgcaa aggacccgta caggccaaag
     1261 gtcacccggt gaaacggatg ctcggcgatg atgcgggcca cccgggccag ctttgccgcc
     1321 cccgcggtgt gggaggagga ggggccgatc atgaccgggc ccagcacctc gaacaggctg
     1381 atgttcatct catgttcccc cttgcgcggc aaacgccgcc cttttctatt actctacccc
     1441 atttgcccgc tttgcgcaag acaaaagcgg cgcgccttcg gcagggcttt tctccctcgg
     1501 cgtccgacga gcggtgttgg agcgcggtga gcgggcggcc cctccttgcc ttttgctcgc
     1561 aaggcgctgc ccccggcggg cgccccgcgg cgcgggccgt cgggagtttt gccccgggcg
     1621 gtgcagcggc ccgcgcaaaa gcgcggcggc ctgccgtttt tcaccctgcc tgcagcaggc
     1681 cccccaacaa aataaaaagc cccaggccca ccagcaccag cggcaccaat acccgctggc
     1741 accggtgcag cggccccttg acccgcggca gggcggcaag gcccgccccc gccgcgcacc
     1801 acagcccggt cagcgccgca aaggccgccg cggcaaggcc catctgggcg gcggagtagc
     1861 cgctgaacac cggcagatac accccgatgt tgtcgccccc gttggcgacg gtcaacaggg
     1921 ccaccgcgaa cacgctcagc cctctccccg ccgcgtcgtc cgctccgtcc ccggcgtctt
     1981 ttctgccgtc gcgccaggcc tttatcccta atgcaagggg cgccaggccc agcagggcca
     2041 cggcgcgccg gggcagcagc caggcgcccc aggcccccag caggctcagg gtggtcagcg
     2101 cgcccatgcc cagatactgc ccggccacga cacgccgccg cccgccgccg tcccgcgcct
     2161 gcccaaagag cagcatcagc aaaaacaggt cgtccaggtt ggtggccaca aaagcggcca
     2221 cagcggaaaa aagcgtcttc gccatcggct gcgcccccct ttcactcgtc ctgcaggccc
     2281 gtattttttg cgcccgggcc gcatagcggg cggcttgtcc ctcgtatgat atcatgtgcg
     2341 gcctcgcctt tcaagcgcgg ccgtttgcac gccggacggc gttggggtat aatggaagct
     2401 gcgaaaagcg gcgcggcggc ccagggccgt cttgggcgcg ctttgtcccc gcctcctcgg
     2461 cggcagacgc agccctcaca aaaccgccgc ccgggccctc ctttgggccc ctgaaacaaa
     2521 agagatccgg gggaagctga tggaaaccgc cacatacacc aaaggtattt tgatcgaaca
     2581 gaccgaatgc gacctgaaaa accgcatgac cttgggggcg ctgctgcgcc gcgcacagca
     2641 ggccgccacc ggccactgcg acgcggtggg ggtgaccacc gcctatctgc tgggctacca
     2701 cgccgccttt ttgctggtaa a
//
LOCUS       DBMDYA010000397         2712 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8205_length_2712_cov_1.472233, whole genome shotgun sequence.
ACCESSION   DBMDYA010000397 DBMDYA010000000
VERSION     DBMDYA010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8205_length_2712_cov_1.472233"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="ACZPFK_09640"
     CDS             <1..1406
                     /locus_tag="ACZPFK_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014871.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1406617.1"
                     /translation="DFSGGAVKVTSTNVREEAMALEPMASVTEVIDRWNAEFDEIKNE
                     VVFKSESNFWGGNIPGLTASNEEVQASVARRGETNAGSLVADARRWKALDWLKENWNS
                     EAYSELGLAEDTPVVVLEGGGSVRGSFRAGDVTLGQLMTAYSFGFENAEDTYVLITPK
                     VMYDMIEHGVNIFLSQDTETGMLEADGSIHGRFPQASGFTYVYDLTQPASSEYDKDAL
                     TMPETIGTRVQSITLEDGTVLDKDDNETKLMLVASAYEIGGGDSYWMLGALNGAENYG
                     GYQYIPEIATPAGNYGDAVADYVNAVYGGTVPADAYPLNSGRITRVNDPYKAAEFTST
                     LTFTDGGKPLASTALTAYINGEKSEVTTDGEGVLTLTLKNGANELRFLGDGYDSGSLY
                     LDNYCGLHNAAVENNPGEVLPDGAEEAPVSSVPAATTPEAPTAQNNTVLIVCLVVLLA
                     VVVAAVAVYFYRKKKSK"
     gene            complement(1591..1989)
                     /locus_tag="ACZPFK_09645"
     CDS             complement(1591..1989)
                     /locus_tag="ACZPFK_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014689.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HIR1406618.1"
                     /translation="MLGFFAGSFFASLINRYAGIVTLAILGVIGMGMIREGVRCSGGS
                     GDCAGRELTLKILLVQAVATSIDAFAVGVSFCAGGADILLAAPLIALTTFACSLLALL
                     VGKRFGEMLGHRAEVFGGIILILIGVKAIL"
     gene            complement(1995..2141)
                     /locus_tag="ACZPFK_09650"
     CDS             complement(1995..2141)
                     /locus_tag="ACZPFK_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406619.1"
                     /translation="MSLWDILLIGLGLSMDAVAVSISNASPIRGCRAPASWPSRRPSA
                     CFRG"
     gene            complement(2445..>2712)
                     /locus_tag="ACZPFK_09655"
     CDS             complement(2445..>2712)
                     /locus_tag="ACZPFK_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406620.1"
                     /translation="ADAAPRGGAEGGKGKRQRRGGKSAAAPAATAPDGALFREKPRHV
                     PPEIQQIRTLPQEWGAPAPAAAPPRRKKSENLLHARPRRTRRQG"
BASE COUNT          518 a          879 c          875 g          440 t
ORIGIN      
        1 tggatttcag cggcggcgcg gtcaaggtca cctccaccaa cgtgcgcgag gaggccatgg
       61 ccctggagcc catggcgtcg gtcacagagg tcatagaccg ctggaacgcc gagttcgacg
      121 agatcaaaaa cgaagtggtc ttcaaatccg agtccaattt ctggggcggc aatatccccg
      181 gcctcaccgc ctccaacgag gaggtccagg ccagtgtcgc ccgccgcggc gagacgaacg
      241 cgggctcgct ggtggccgac gcccggcgct ggaaagcgct ggactggctg aaagagaact
      301 ggaactccga ggcgtacagc gagctgggcc tggcggagga cacgccggtg gtggtgctgg
      361 agggcggcgg cagcgtgcgc ggctcgttcc gcgccggtga cgtgaccctg ggccagctga
      421 tgaccgccta ctcctttggc tttgagaacg cggaggacac ctatgtgctc atcaccccca
      481 aggtgatgta cgatatgatc gagcacggcg tgaacatctt cctctcccag gataccgaga
      541 ccggcatgct ggaggcggac ggctcgatcc acggccgctt cccgcaggcg tccggcttca
      601 cttacgttta cgacctcacc cagccggcca gcagcgagta cgacaaggac gcgctgacca
      661 tgccggagac catcggcacc cgcgtgcagt cgatcacgct ggaggacggc accgtgctgg
      721 acaaagacga caacgagacc aagctgatgc tggtggcgag cgcctatgag atcggcggcg
      781 gcgacagcta ctggatgctg ggcgcgctga acggggcgga gaactacggc ggctatcagt
      841 atatccccga gatcgccacc ccggccggca actacggcga cgctgtggcc gattatgtga
      901 acgcggtgta cggcggcacc gtgccggcgg acgcctatcc cctcaacagc ggccgcatca
      961 cccgggtcaa cgacccctac aaggccgctg agttcacctc gaccctcacg ttcaccgacg
     1021 gcggcaagcc gttggcgagc accgccctca ccgcctatat caacggcgag aagagcgagg
     1081 tcaccacgga cggcgagggc gtgctgacgc tgacgctgaa aaacggcgcc aacgagctgc
     1141 gcttcctcgg cgacgggtat gacagcggtt ctctttatct ggacaactac tgcggcctgc
     1201 acaacgcggc ggtggagaac aatcccggcg aggtgctccc cgacggcgct gaagaggctc
     1261 ccgtctcctc ggtgcccgca gcgaccacgc cggaggcccc gacggcgcag aacaacaccg
     1321 tgctgatcgt ctgcctggtg gtgctgctgg cggtggtcgt ggcggccgtg gccgtctact
     1381 tctatcgcaa gaaaaagagc aaataagggc ggcggtaaac cggcgcttga tcgaaaagct
     1441 ttataaaagg cggccgctga tgcggccgcc ttttttgccc tcgcccggca aagaggcctt
     1501 ttccgccggg atcgggcggc atgcgccttt catcggggct gcccggcccg caccgtccgc
     1561 cgtccttggt ccacgccggt cttttcgccc tcacaggatg gccttgaccc cgatcaggat
     1621 caggatgatg cccccgaaga cctcggcccg gtggcccagc atctcgccga agcgtttacc
     1681 caccagcagc gccagcaggc tgcaggcaaa ggtggtcagc gcgatgagcg gcgcggccag
     1741 caggatatcc gccccgcccg cgcaaaagct cacccccacg gcaaaggcgt cgatgctggt
     1801 ggcgaccgcc tgtaccagca gtatctttag agtcagctct ctgcccgcgc agtcgccgga
     1861 gccgccgctg cagcgcacgc cctcgcggat catccccatg ccgatcacac ccaggatcgc
     1921 cagcgtcacg atgcctgcat agcggttgat gagggaggca aagaagctgc ccgcgaaaaa
     1981 gcccagcacc ggcatcaccc cctgaaacag gccgaaggcc gccgggatgg ccagctggcg
     2041 ggagcgcgac agccccggat aggcgaggcg ttggagatgg agacggccac tgcgtccatc
     2101 gagaggccga ggccgatcag caaaatgtcc cataaactca tcttgatctc tcctgttttt
     2161 tcggtgtgat gtggtcgaaa aaggggcggc cggtgcggca aatgcaaaag cccatcaaaa
     2221 aagacctatg tatagccggt gaaagctata cataagtctc gttttaaaca caagccaggc
     2281 aagcattttg ccagaatgtt gacttgggaa cgcggaggta ccgcgcaaac tactccccta
     2341 tgtgaaaaaa atatctatcc tattttctca taatttgccg atttgtcaag agcgcgcccg
     2401 caaaaaggcc gctggccgtg cgtgcgcgcc gggccggagc tttttcagcc ctgccgccgg
     2461 gtgcggcggg gacgcgcgtg cagcagattt tccgattttt tgcgccgggg cggcgcagcg
     2521 gcaggggccg gggcgcccca ctcctggggc agggtgcgga tctgctggat ctccggcggc
     2581 acatggcgcg gtttttcccg gaaaagggcg ccgtcgggcg ccgtggcggc gggcgctgcc
     2641 gcgcttttgc cgccgcgcct ttgccgcttg cccttgccgc cctccgcccc gccgcggggg
     2701 gctgcgtccg cc
//
LOCUS       DBMDYA010000398         2707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8217_length_2707_cov_1.749424, whole genome shotgun sequence.
ACCESSION   DBMDYA010000398 DBMDYA010000000
VERSION     DBMDYA010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_2707_cov_1.749424"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="ACZPFK_09660"
     CDS             complement(<1..477)
                     /locus_tag="ACZPFK_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019194.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HIR1406621.1"
                     /translation="MNKILREKIGEAVASVLPITSIVLALSVTVAPMPLGTVMMFLAG
                     ALLLVVGAGLFSLGADIAMMPLGEAMGARLGGSRRWPLVALVSLALGALITLAEPDLQ
                     VLADQVPAIPDAVLIFTVAAGVGAFLVLAMLRTRLGWPLSRLLAFFYPLVFLLAFLA"
     gene            complement(736..1611)
                     /locus_tag="ACZPFK_09665"
     CDS             complement(736..1611)
                     /locus_tag="ACZPFK_09665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1406622.1"
                     /translation="MKKIGFIGVGVMGAPMVQNLMRAGYELTVYSRTKAKCDAVLQAG
                     AKWADSPAACAAGQDAVLTMVGYPKDVEQVYFGENGVFESVKKGMLLIDLTTTSPRLA
                     KRIYKHAQEAGAQALDAPVSGGDVGAQKGTLAIMVGGDEDAFARAEPLLKAMGSNVVY
                     EGPAGSGQHTKMANQIAIAGAVSGVCEALAYGRAQGLDLDRMLATIGSGAAGSWQMSG
                     NGPKMLAGDFAPGFYIKHFIKDMKIAEEEAESAGLELGILSDVLMMYEELEEQGMGDL
                     GTQALLKYYEPEDGE"
     gene            complement(1687..>2707)
                     /locus_tag="ACZPFK_09670"
     CDS             complement(1687..>2707)
                     /locus_tag="ACZPFK_09670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1406623.1"
                     /translation="QLEGASLSLPQGYRFLSIQVDGTGGTVFSGSEEEYSSFAYPGNG
                     RYLYRIQVEAPPAEEGERARAHGTLSYKFTVKVSVELNAFLSDERVKQGEIVAVRIEN
                     NLDGVQPFGESALGPVNFIPSGEDGWVAFVPVAYNREAGDYTVEVSCGDFSASLPVKV
                     EYKPYEKKEFASAADLPDAAEESAAASSQYRDAIWPLYDAMRPLVLWQGIFQKPVEGL
                     IKYEYGMGSLLPGAAVSQRHSGVDYTVAADNTPVTAPGGGEVFYAGNLVLTGNTVVIE
                     HGGGVKSYLYHLSSIAVTGGQTVEKGQQIGTLDSAGVLHYEIKIGNQSVDPAPVFSGQ
                     SGLYR"
BASE COUNT          481 a          962 c          749 g          515 t
ORIGIN      
        1 ggccaaaaac gccagtaaaa acaccagcgg ataaaaaaac gccagcaggc gggacagcgg
       61 ccagcccagc ctggtgcgca gcatagcgag caccagaaag gcccctaccc ccgccgcaac
      121 cgtaaagatc agcaccgcgt cggggatggc cggcacctga tccgccagta cctgcaggtc
      181 cggctcggcc aacgtgatca gcgcgcccag ggccaggctg accagcgcca ccagcggcca
      241 gcggcggctg ccgcccagcc gcgcgcccat cgcctccccc aagggcatca tcgcgatgtc
      301 cgcccccagg gaaaaaaggc ccgcccccac caccagcagc agggcgcccg ccagaaacat
      361 catgacggtt cccagcggca tcggcgccac ggtgacgctg agcgccagca cgatgctggt
      421 gatgggcagg accgaggcca ccgcctcgcc gatcttctcc cttaatatct tgttcatagc
      481 tcaccttctt ttctgcgcct cggccgcagc ccggccaaat gtaaactttt atttataatt
      541 acagtataac agatcatgtg gtcctgtttt atgaactttt tgtaaactgt cgcctcccgc
      601 acctttcttt tctgtttttt cagtttatga aaaaccaggt ttttctattc gaaatagccc
      661 ttatggacag aaaagcggcc gggctttggc gcccagccgc ttttctgaaa ggccataatc
      721 tgtgactttt atcctctact ccccgtcctc cggctcgtag tacttcagca gggcctgtgt
      781 gcccaggtcc cccatgccct gttcctccag ctcctcatac atcatcagca catccgagag
      841 gatgcccagc tccaggccgg cgctctccgc ctcctcctcg gcgatcttca tatccttgat
      901 aaaatgcttg atgtaaaagc cgggggcaaa gtcgcctgcc agcattttgg ggccgttgcc
      961 cgacatctgc cagctgcccg ccgcgccgct gccgatggtg gccagcatcc tgtccaggtc
     1021 cagcccctgc gcccggccgt aggccagggc ctcgcagacg ccggacaccg cgcccgcaat
     1081 ggcgatctgg ttggccatct tggtgtgctg gccgctgccc gccgggccct catagaccac
     1141 gttgctgccc atggctttca gcaggggctc cgcgcgcgca aaagcatcct catcgccgcc
     1201 caccatgatg gccagcgtcc ccttttgggc gcccacgtcg cccccggaca ccggcgcgtc
     1261 caacgcctgg gcccccgcct cctgcgcgtg cttgtagatc cgctttgcaa ggcgcgggct
     1321 ggtggtggtc aggtcgatga gcagcatccc ttttttcacg ctctcaaaaa cgccgttctc
     1381 accgaagtag acctgctcca catccttggg gtagcccacc atggtcagca ctgcgtcctg
     1441 gcccgcggcg caggccgccg ggctgtccgc ccattttgcc cccgcctgca gcaccgcgtc
     1501 acattttgcc ttggtgcggg aatagaccgt cagctcatac cccgccctca tcaggttctg
     1561 caccataggc gcccccatca cgcccacgcc gataaatccg atctttttca tctctccgcc
     1621 tcctatcatc ataagcgccg attcaacgga gggaagcgcc ttgacgccgc ccgccgcgcg
     1681 cacggctcac cgataaagcc cgctctgccc cgaaaaaacg ggggccgggt ccacgctctg
     1741 gttgccgatc ttgatctcat agtgcaggac gccggcagaa tccagcgtgc cgatctgctg
     1801 ccctttttcc accgtctgtc ccccggtcac tgcgatgctg gacaggtggt agagatagct
     1861 tttgaccccg ccgccgtgct cgatcaccac cgtgttgccg gtgaggacca gattgcccgc
     1921 atagaacacc tcgcccccgc cgggggcggt caccggcgtg ttgtccgccg caacggtata
     1981 gtcgacgccg ctgtggcgct gcgagaccgc ggccccgggc agcaggctgc ccatgccgta
     2041 ctcgtatttt atgagcccct ccaccggctt ttgaaagatg ccctgccaga gcacaagggg
     2101 gcgcatggcg tcgtagaggg gccagatggc gtcccggtac tggctgctgg ccgccgctga
     2161 ttcctctgcg gcgtccggca ggtcggcggc tgaggcgaac tcttttttct catagggctt
     2221 gtattccact ttgaccggca ggctggccga aaaatcgccg cagctcacct ccaccgtgta
     2281 gtcgcccgcc tcccggttat aggccaccgg cacaaaggcc acccagccgt cctcgccgga
     2341 ggggataaaa ttgaccgggc ccagcgcgct ctcgccgaac ggctgcaccc cgtccagatt
     2401 gttttcgatc cgcacggcca cgatctcgcc ctgcttcact cgctcgtcgg agagaaaggc
     2461 gttcagctcc accgagacct tgacggtgaa cttgtagctc agggtgccgt gcgcgcgggc
     2521 gcgctccccc tcctctgcgg gcggggcctc gacctggatg cgatacagat agcgcccgtt
     2581 gcccggatag gcgaaagagc tgtactcctc ctctgagccg gaaaagacgg tgccgcccgt
     2641 gccgtccacc tggatggaca aaaagcggta tccctgcggc agcgaaagag acgcgccctc
     2701 cagctgg
//
LOCUS       DBMDYA010000399         2705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8225_length_2705_cov_1.605223, whole genome shotgun sequence.
ACCESSION   DBMDYA010000399 DBMDYA010000000
VERSION     DBMDYA010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_2705_cov_1.605223"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="ACZPFK_09675"
     CDS             complement(<1..1241)
                     /locus_tag="ACZPFK_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1406624.1"
                     /translation="MSKQYEQEIRRRRTFAIISHPDAGKTTLTEKLLLYGGAIQLAGT
                     VKGKKTQRAAVSDWMEIEKQRGISVTSSVMQFNYDGYCINILDTPGHQDFSEDTYRTL
                     MAADSAVMVIDAAKGVERQTEKLFKVCLLRDIPIFTFINKMDRESLNPFDLLDDIEQK
                     LGIRTYPMNWPIGSGKGFKGVYNRENREILSYGDEAGSALGGQHKLQTVKATLGDPGL
                     DGLIGAAQHQTLADDIELLDGASYDFDLDAIRHAQLSPVFFGSALTNFGVEPFLQDFL
                     RLTTPPLPRRCDEGEVDPMDDDFSAFVFKIQANMNKAHRDRVAFMRICSGRFEKNMEV
                     LHVQQDRRLRLSQPQQILASEREIIEEAYAGDIIGVFDPGVFSIGDTLCAPGKKFRFE
                     GIPTFAPEHFSLVRQVDTMKR"
     gene            complement(1516..2532)
                     /locus_tag="ACZPFK_09680"
     CDS             complement(1516..2532)
                     /locus_tag="ACZPFK_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406625.1"
                     /translation="MSKSSMRDVARHAGVSVATVSHVINNTRFVAPDTRARVLESIRQ
                     LDYSPDAMARSFKTGRKNLVGFIVPDIANEFFSTIIEEAESVIAKKNMRLIVVNTKET
                     KQREADHLHALSSGIVDGLIVASTMESYGEFAKTLSTPLPMVFIDRSLQGCPCDTITI
                     SNYDSIYAGMETLIREGHTRIGYIAGLMRLSTTAERLAAYRDAMEDYRLPVEEGFVQY
                     GDSMSRSAVPHVARLLELGCTAVVVGNNVMADDVLYYLNEHDVKIGHGFSMIGYDDTD
                     KANYNMRRMHMVRQPAKELGRTAGKQILERIECPEGPVKNIVLHADFVPRRSPILAGS
                     TAGY"
BASE COUNT          473 a          837 c          819 g          576 t
ORIGIN      
        1 ttgcgcttca tggtgtccac ctggcgcacc agggagaagt gctcgggcgc aaaagtgggg
       61 atgccctcaa agcggaactt tttgcccggc gcgcagaggg tgtcgccgat ggagaagacg
      121 cccgggtcga acacgccgat gatgtcgccg gcataggcct cctcgatgat ctcccgctcg
      181 gaggccaaaa tctgctgcgg ctgcgagagg cgcagcctgc ggtcctgctg gacgtgcagc
      241 acctccatat tcttttcaaa ccggcccgag cagatgcgca taaacgccac tctgtcccgg
      301 tgggccttgt tcatgttggc ctggattttg aacacgaaag ccgagaagtc gtcgtccatg
      361 gggtctacct ccccctcgtc gcagcggcgg ggcagcggcg gggtggtgag gcgcagaaag
      421 tcctgcaaaa acggctccac accaaagttg gtcagcgcgc tgccgaagaa gacgggcgag
      481 agctgggcgt ggcggatggc gtccagatcg aagtcatagc tggcgccgtc cagcagctcg
      541 atgtcgtcgg ccagggtctg gtgctgtgcg gccccgatga ggccgtccag gcccgggtcg
      601 cccagcgtgg ccttgacggt ctgcagcttg tgctggccgc ccagggcgct gcccgcctcg
      661 tcgccgtagg acaagatttc ccggttctcg cggttgtaca cgcccttgaa ccccttgccg
      721 ctaccgatgg gccagttcat gggataggtg cggatgccca gcttctgctc gatgtcgtcc
      781 agcaggtcaa aggggttcag cgactcccgg tccatcttgt tgataaaagt aaagatgggg
      841 atgtcccgca gcagacagac cttgaacagc ttttcggtct gccgctccac gcccttggcc
      901 gcgtcgatca ccatcaccgc gctatcggcc gccatcaggg tgcggtaggt gtcctctgaa
      961 aagtcctggt gccccggggt gtccaggatg ttgatgcagt agccgtcgta gttgaactgc
     1021 atcaccgagc tggtgaccga gatgccgcgc tgcttttcga tctccatcca gtcggacacg
     1081 gcggcccgct gggtcttttt gcccttgacc gtgcccgcca gctggatggc gccgccgtag
     1141 agcagcagct tttcggtgag ggtggtcttg ccggcgtccg ggtggctgat gatggcgaac
     1201 gtgcgccgcc gccggatctc ctgttcatat tgtttggaca actttaagtt cctcccgctc
     1261 gcagaatggg tgggccgccc ctgcgggcgg acatacggta ttattttatc tcgcaggcgc
     1321 tcgaaaagca agaattttgt gggtgaaaat tcccgccgcg ccgccgggcg gccccagcgc
     1381 tgcggcgggg aggggcggtg agctgcttcc cgctccgccg ctttttcagc gcgccggcct
     1441 cccggcttct cagcagataa aagggccgtc ttctaaaaag aagacggccc tttcatctgc
     1501 ttgcgtctcc gcttgtcaat accccgccgt gctcccggcc aaaatcggcg agcgccgggg
     1561 cacaaaatca gcgtgcagca caatgttctt caccggcccc tccgggcatt cgatccgctc
     1621 gaggatctgt tttcccgccg tgcgccccag ctcttttgcg ggctggcgca ccatgtgcat
     1681 acggcgcatg ttgtaattgg ccttgtcggt atcgtcgtaa ccgatcatcg aaaagccgtg
     1741 gccgatcttg acgtcgtgct cgttgagata gtacagcacg tcgtccgcca tgacattgtt
     1801 gcccaccacc accgcggtgc agcccagctc cagcaggcgg gcaacatggg gcaccgcgct
     1861 gcggctcatc gaatcgccgt actgcacaaa gccctcctcc accggcagcc ggtagtcctc
     1921 catggcgtcc cggtaggcgg caaggcgctc cgccgtggtg gacagccgca tcaggcccgc
     1981 gatgtagccg atgcgggtat gcccctcccg gatcagggtc tccatcccgg catagatgga
     2041 atcatagttt gaaatggtga tcgtgtcgca ggggcagccc tgcagcgagc ggtcgatgaa
     2101 caccatgggc agcggcgtgg aaagggtctt ggcaaactcc ccatagctct ccatggtgct
     2161 ggccacgatc agcccgtcca caatgccgct ggacagcgcg tgcagatggt ccgcctcccg
     2221 ctgcttggtc tcctttgtgt tgaccacgat cagccgcata ttctttttcg cgatgacgct
     2281 ctccgcctct tctataatgg tggaaaagaa ctcgttggca atatcgggga cgataaagcc
     2341 gaccaggttt ttgcgtccgg tcttaaagct gcgcgccatc gcgtcggggc tgtaatccag
     2401 ctgccggatg ctctccagca cccgggcccg ggtgtccggc gcgacaaagc gggtgttgtt
     2461 gatgacatgg ctcacggtgg ccacggatac cccggcatgc cgcgctacat ctctcatact
     2521 cgattttgac attgctctca tccttgcgcc cggcgatccc gcggcattgc cgcggggctc
     2581 ttcagcctaa aaaagccccg atcgcctgtt ctcattttta aaacgggagt gggccatccg
     2641 caaccgttta ctttaactgt taaatcaagt atagatgttc tttcctatat ttgatttaac
     2701 agtta
//
LOCUS       DBMDYA010000400         2703 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8239_length_2703_cov_1.349347, whole genome shotgun sequence.
ACCESSION   DBMDYA010000400 DBMDYA010000000
VERSION     DBMDYA010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_2703_cov_1.349347"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="ACZPFK_09685"
     CDS             <1..438
                     /locus_tag="ACZPFK_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1406626.1"
                     /translation="LVQGLVERDPGGYLLVNEKKETSLPGLYAVGDCTRTFLRQVVTS
                     AADGAVAATASERYVQELQQLDDILTPGSGDVAFLFYDPYHSEEIDCVGALERKLCKT
                     WKVYRQDITRQDLLYRELKPGRTVCAAFYRDGKLLEVKAAEDC"
     gene            504..2312
                     /locus_tag="ACZPFK_09690"
     CDS             504..2312
                     /locus_tag="ACZPFK_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1406627.1"
                     /translation="MGQPLVFPHGVTVYDPEKCWNGYTLVPLINDGVLLFDMNGNEVR
                     RWNMHAMPPKLLPGGYVMGLSGYRHPDYGMQDGVNLIEIDYDGNIVWEFDHLEEIDDP
                     GRDHRWMARQHHDYQREGNPVGYYVPGMEAKPLQGNTLILCHQTIRNPLISDKKLLDD
                     VMIEVDWEGNILWKWSISDHFDELGFDEAAKNVLFRDPNLRASDGGVGDYLHVNCMSY
                     LGPNKWYDQGDQRFKPDNIIFDCREANVLAILDKESGKIVWRLGPDFSATPELRKLGW
                     IIGQHHFHMIPRGLPGEGNLLVFDNGGWAGYGVPNPASPIGIKNALRDYSRVLEFNPV
                     TLEVVWKLTPKELGHAIPTDASKFYSPYVSSAQRLPNGNTLIDEGSDGRVIEVTPEHE
                     IVWEWISPYYSHNEKGPRSNMIYRAYRYPYSYVPQEPVPTQVPIPRIDNTTYRVPGAG
                     AFGAKKVVDVPGTLPYYQDVALCVATDDEEDVSKREKIFEVDTGFFTPVTRENWDREV
                     LKVRGTPVLVLFGAERCTHCKALHPVLEEALKEEYEGQFAVRYVDVDANPELTAASHV
                     GGIPVVVVYRDGAEAVRFNGEKDYDDLCDLLDRALQ"
     gene            2445..>2703
                     /locus_tag="ACZPFK_09695"
     CDS             2445..>2703
                     /locus_tag="ACZPFK_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1406628.1"
                     /translation="MRGSFFSGLDAGQPSFTRLCLSGALCDRYRSYFLTRVYPAGQCI
                     HAQGEEITHFGLVTAGILKAVTCSATGEELCGSYFVEGNSFP"
BASE COUNT          533 a          843 c          828 g          499 t
ORIGIN      
        1 cttgtgcagg gcctggtcga gcgcgacccg ggcggctatc tgctggtgaa cgaaaagaaa
       61 gagaccagct tgcccggcct gtatgcggtg ggcgactgca cccgcacctt tctgcgtcag
      121 gtggtcacct cggccgcgga cggcgcggtg gcggccaccg cgtccgagcg ctatgtgcag
      181 gagctgcagc agctggacga tatcctgacg ccgggctccg gcgacgtggc ttttctcttt
      241 tacgacccct atcacagcga ggagatcgac tgcgtgggcg cactggagcg caagctgtgc
      301 aaaacctgga aagtctatcg ccaggacatc acccggcaag acctgctcta ccgggagctg
      361 aagccggggc gcaccgtctg tgcggcgttt taccgcgacg gcaagctgct ggaggtcaag
      421 gcggccgagg actgctgaac ctccgctttt ttgcctttcg atgttcaaat cagaccatct
      481 gattcaaaag gaggatgctc attatgggcc aaccgcttgt tttcccccac ggggtcaccg
      541 tctacgaccc tgaaaaatgc tggaacgggt acacgcttgt tccgctcatc aacgacggcg
      601 tgttgctgtt cgacatgaac ggcaacgagg tgcgccgctg gaacatgcac gccatgcccc
      661 ccaagctgct gcccggcggc tatgtgatgg ggctctcggg ctatcgccac cccgactacg
      721 gcatgcagga cggggtaaac ctgatcgaga tcgactacga cggcaatatc gtgtgggagt
      781 tcgaccacct ggaagagatc gacgacccgg ggcgcgacca ccgctggatg gcgcgccagc
      841 atcacgacta ccagcgcgag ggcaacccgg tagggtatta tgtgcccggc atggaggcaa
      901 agccgctgca gggcaacacg ttgatcctgt gccaccagac catccgcaat ccgctgatct
      961 cggacaaaaa gctgctggac gatgtgatga tcgaggtgga ctgggagggc aatatcctct
     1021 ggaaatggtc catcagcgac catttcgacg agttgggctt tgacgaggcc gccaaaaacg
     1081 tgctgttccg cgaccccaac ctgcgcgcct ccgacggcgg ggtgggggat tatctgcatg
     1141 tgaactgcat gagctacctg ggtcccaaca aatggtacga ccagggcgac cagcggttca
     1201 agcccgacaa tatcattttc gactgccgtg aggccaatgt gctggcgatc ctggacaaag
     1261 agagcggcaa gatcgtctgg cgcctcgggc cggatttcag cgccacgccg gagctgcgca
     1321 agctgggctg gatcatcggc cagcaccatt ttcatatgat cccccggggc ctgcccggcg
     1381 agggcaacct gctggtgttc gacaacggcg gctgggccgg ctacggcgtg ccgaaccccg
     1441 cctcccccat cggtatcaaa aatgccctgc gggattacag ccgcgtgctg gagttcaatc
     1501 ccgtcacgct ggaggtcgtc tggaagctca ccccgaaaga gctgggccac gccatcccca
     1561 ccgacgccag caaattctac agcccctatg tgagcagcgc ccagcgtctg cccaacggca
     1621 acaccctgat cgacgagggc tcggatgggc gggtcatcga ggtcaccccc gagcacgaga
     1681 tcgtgtggga gtggatttcc ccctactaca gccacaatga aaaggggccc cgcagcaata
     1741 tgatctaccg cgcctaccgc tatccctaca gctacgtgcc gcaggagccc gtccccaccc
     1801 aggtccccat cccgcgcatt gacaacacga cctaccgcgt ccccggcgcg ggcgctttcg
     1861 gcgcaaaaaa agtggttgat gttcccggca ctttgccgta ttatcaagat gtggctttgt
     1921 gcgtggctac cgacgacgaa gaagatgtga gcaagcggga aaagattttt gaggtggaca
     1981 ccggcttttt cacgcccgtg acccgtgaaa actgggaccg ggaggttttg aaagtgcggg
     2041 gcaccccggt gctggtgctg ttcggcgccg agcgctgcac ccactgcaag gcgctgcatc
     2101 cggtgctgga ggaagcgctg aaagaggagt atgagggcca gttcgccgtc cgctatgtgg
     2161 atgtggacgc caacccggag ctgaccgcgg ccagccatgt gggcggcatc ccggtggtcg
     2221 tcgtctaccg cgacggggcc gaagcagtcc gcttcaacgg cgaaaaggac tacgacgacc
     2281 tctgcgacct gctcgaccgc gccctgcaat gagcatccca gggcaacggc attttaggcc
     2341 ttgccgcccg taccgacgca caatataaaa agccacagag ggaagaactg ccccgcaaaa
     2401 ggcggcgctc ccctctgtgg tatttttttg tgaggtggac cattttgcgc ggctcttttt
     2461 tttccggtct ggacgccggc cagccctctt tcacccggct ctgtctcagc ggcgccctct
     2521 gcgaccgcta ccgctcttac tttctcaccc gcgtttatcc cgcagggcag tgcatccacg
     2581 cccaggggga ggagatcacc cattttgggc tcgtcaccgc gggcatttta aaggccgtca
     2641 cctgctccgc caccggggag gagctgtgcg gcagctattt tgtggagggg aacagcttcc
     2701 ccg
//
LOCUS       DBMDYA010000401         2699 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8258_length_2699_cov_1.486528, whole genome shotgun sequence.
ACCESSION   DBMDYA010000401 DBMDYA010000000
VERSION     DBMDYA010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_2699_cov_1.486528"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(281..1531)
                     /locus_tag="ACZPFK_09700"
     CDS             complement(281..1531)
                     /locus_tag="ACZPFK_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406629.1"
                     /translation="MKKQGSVHYAWVVVFCCVMMSFTSMIANAACGNFTTPVVQELGC
                     SVSSFTLFISIQAGSMALMYPIAAKVLTTKPIGKVVTAALLLQLIALGLMGTYHSVYS
                     FYLSGVLSGVGSAFSYLIALPIIINMWFKEKAGLALGIVTSFGSAFGILSSLLSAQLI
                     VHVGWRNAYFILALCGMVSLPVVFFLLKTPQEKNVLPYGAGQSAGGAAAQGGGEWGLT
                     RKQAFRHPMFYLAWLTCLCYSLGSGVPSYSATFATMELGQSIQFGSYAAVCVSLGMIA
                     CGFILGFINDRFGARAGMIWGAAFNVAGFSVMMLAIRSPMLVLPGCILLGLGGSMYTV
                     QAPLIARSVLGPKHYSEIWSVMMIGNSLMGAFSFGPIGLFYDKTGSYRGAFIMAMAVF
                     TAAVFVGSAALSLSQKYRREHGAA"
     gene            complement(1686..2111)
                     /locus_tag="ACZPFK_09705"
     CDS             complement(1686..2111)
                     /locus_tag="ACZPFK_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406630.1"
                     /translation="MREKLKNLLGFRSNTPWKKIVAVLYYLLCLTVWVVGLVTPLPIE
                     AGLWDVFVYKLSVTVIFLWMVSPAIFLSETPLRRRLPLFRQRIGSKSLMGMMIVFILF
                     TYLFAMTESWHTPEYKAAYEAYNTAAYNAFIVAGGGQVP"
     gene            complement(2185..>2699)
                     /locus_tag="ACZPFK_09710"
     CDS             complement(2185..>2699)
                     /locus_tag="ACZPFK_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1406631.1"
                     /translation="WNCITPSLAVAKATGADFTRCTALVSSTDSMFGRVYAEPLKVME
                     YRKKIDAQGVGMISEISGYHFKGAYEKKNLQNLAASSIKVGASAVEVMNGDFEMNERL
                     VKDIKELGDIPVILGGGTTVENCKDRLRYADGALVGSAFEGGKWGGPVIESIVAEYVK
                     RVRELEKELA"
BASE COUNT          530 a          838 c          878 g          453 t
ORIGIN      
        1 cgccgcgcct cctgaggcgg gcccggccgc gcggcttcaa aagacggcaa aacacaggca
       61 gccggcgctc ccgacggggg acgccggctg ttttctgtcg acggctttgc cgccggagac
      121 gggccgcggt acagaggaga cggccgcggg ctgttttccg ccgcttcgca gtaaaatttc
      181 tgatctcccc ggcttcgctt cccgccgccg tcaccgcggc gaaagcggag agccgggagg
      241 acggccgccg cgcggacgcc tttcaaggcg ttgcggcggg tcaggcggcc ccgtgctccc
      301 ggcggtattt ttgggagagg gagagggcgg cgctgcccac aaagaccgcc gcggtgaaca
      361 ccgccatggc catgataaag gcgccgcggt agctgccggt cttgtcatag aacaggccga
      421 tggggccaaa ggagaacgcg cccatcaggc tgttgccgat catcatgacc gaccagatct
      481 ccgagtaatg cttgggcccc agcacgctgc gggcgatcag cggcgcctgc accgtgtaca
      541 tgctgccccc gaggcccagc aggatgcagc ccggcaggac cagcatcggg ctgcggatgg
      601 ccagcatcat gacgctgaag ccggccacat tgaacgcggc cccccagatc atgcccgccc
      661 tggcgccgaa gcggtcgttg ataaagccga ggatgaagcc gcaggcgatc atgcccaggc
      721 tgacgcagac ggccgcataa gagccgaact ggatcgactg gcccagctcc atggtggcga
      781 aggtggccga gtagctgggc acgcccgagc ccaggctgta gcacaggcag gtcaaccagg
      841 ccagatagaa catcgggtgg cggaacgcct gcttgcgggt gaggccccac tcgccgccgc
      901 cctgggccgc ggcgccgccc gccgattggc cggcgccgta ggggagcacg tttttctcct
      961 gcggggtctt gagcagaaag aagaccaccg gcagagagac catcccgcac agggccagga
     1021 tgaagtaggc gttgcgccag cccacatgca cgatcagctg ggcggagagc aggctggaca
     1081 ggatgccgaa ggcgctgccg aaagaggtga cgatgcccag ggcgagacct gccttttctt
     1141 tgaaccacat gttgatgatg atgggcaggg cgatcaggta ggagaaagcg gagccgacgc
     1201 ccgagagcac gccggacagg taaaagctgt agaccgagtg gtaggtgccc atcagcccca
     1261 aagcgatgag ctgcagcagc agcgccgcgg tcaccacctt gccgatgggc ttggtggtca
     1321 gcaccttggc ggcgatgggg tacatcagcg ccatagagcc cgcctggatg ctgatgaaaa
     1381 gggtgaaaga gctgaccgag cagcccagct cctgcaccac cggggtggtg aaattgccgc
     1441 aggcggcgtt tgcgatcatg ctggtgaacg acatcatcac gcagcagaac accaccaccc
     1501 aggcgtagtg gaccgaacct tgttttttca tctgagatcc cccttgaaaa gaatgcgttt
     1561 ccctcccggc aaagctccgt gcggccggga acgcagaaaa aagaacgggc cgtcctcccc
     1621 atggctgatg cgggggaaaa cggcccgttt tccattttga agcagccgcg gcgcccctgc
     1681 cccgcttagg gcacctgccc gccgccggcc acgatgaaag cgttgtaggc cgcggtgttg
     1741 taggcctcat aggcggcctt gtactccggc gtgtgccagc tctcggtcat ggcgaacaga
     1801 taggtgaaaa ggataaaaac gatcatcatg cccatcagcg atttggagcc gatccgctgc
     1861 ctgaagagcg gcagccgccg gcgcaggggt gtttcggaga gaaagatcgc cgggctgacc
     1921 atccacaaaa agatcaccgt caccgagagc ttatagacga aaacgtccca gaggcccgcc
     1981 tcgatgggca ggggggtgac gagccccacc acccagacgg tcaggcagag caggtaatag
     2041 agaaccgcga cgatcttttt ccagggcgtg ttggagcgga agcccagcag atttttcaat
     2101 ttttccctca tgacaactat cctttttccc tattgatgcg ttttgatgtg tccggcgcgg
     2161 agccgccgcc ttgcaaagcg cgcttcaggc gagttctttt tccagctcgc gcacccgctt
     2221 gacgtactcg gcgacgatgg attcgatgac cgggccgccc catttgccgc cctcgaacgc
     2281 gctgcccacc agggcgccgt cggcgtagcg caggcggtcc ttgcagtttt ccacggtggt
     2341 gccgccgccc aggatgacgg ggatgtcgcc cagctccttg atgtccttga ccagacgctc
     2401 gttcatctca aaatcgccgt tcatcacctc gacggcgctc gcgcccacct tgatggagct
     2461 ggcggccagg ttctgaaggt tctttttttc ataagcgccc ttgaagtggt agccggagat
     2521 ctcggagatc atgcccacgc cctgcgcgtc gatctttttg cgatattcca tcaccttcag
     2581 cggctcggcg tacacgcggc cgaacatcga gtcggtggag ctgaccaggg cggtgcagcg
     2641 ggtgaagtcg gcgccggtgg cctttgccac ggccagcgag ggggtgatgc agttccaca
//
LOCUS       DBMDYA010000402         2692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8285_length_2692_cov_1.713624, whole genome shotgun sequence.
ACCESSION   DBMDYA010000402 DBMDYA010000000
VERSION     DBMDYA010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_2692_cov_1.713624"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..1281)
                     /locus_tag="ACZPFK_09715"
     CDS             complement(61..1281)
                     /locus_tag="ACZPFK_09715"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1406632.1"
                     /translation="MVGGGAAGMLAAGTAARRGLEVTVVEHMERTCKKILVTGKGRCN
                     VTNDCDPAAVLKNVRGNPKFLYSALNAFTPADTMALMEALGVPLKTERGRRVFPVSDR
                     AADVADALARYARGCKVLFAEAAAVEAADGAAVGVRLADGRSLPADGVILATGGRSYP
                     GTGSTGAGYRMAQALGHTVVPTTASLVALAVGGPARQECRAMMGLSLRNVRLTLLEGD
                     KPLYTEQGEMLFTHFGISGPLVLSASAYLRGDAVRGVRAVIDLKPALDEPTLYARVNR
                     DFEKFANRDAGNSLSGLLPAGMVPVMLARWGVPPGKKVNQVTREERRRLTGLLKGFEL
                     PISGKDAIERAVVTAGGVSVREVSPRTMESKLVKNLHFAGELLDVDAYTGGYNLQIAF
                     STGYAAGTHILEEK"
     gene            complement(1499..2071)
                     /locus_tag="ACZPFK_09720"
     CDS             complement(1499..2071)
                     /locus_tag="ACZPFK_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR1406633.1"
                     /translation="MKNTTIIYGLSGSFCNFEHSLKALEELVRQGAEVIPAMSFNAAS
                     LDTRFGTAAEFRRRLVEITGHEIIDTIQAAEPVGPKKLCDLLVVSPCTGNTLSKLACG
                     ITDTPITMACKSHLRCGRPVVLALASNDSLSASAQNLGRLLNTKNYYFVPLEQDDWLK
                     KPSSLVADFSQLPATVESALRGRQVQPIFF"
     gene            complement(2074..>2692)
                     /locus_tag="ACZPFK_09725"
     CDS             complement(2074..>2692)
                     /locus_tag="ACZPFK_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013430.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1406634.1"
                     /translation="ALLRAAKPGAIAYAGKVSGEAAAVAQAAGVELDDYLQREELAVM
                     NAIPTCEGAVEILLRERQATLWNSRVLITGFGRIAKLLCQRLTGFGALVTVAARKPRD
                     RALASALGYRTQTIDALRETVRGFDVIVNTVPAPLIDREMIGTMGSGAFLLDLASAPG
                     GIDLAAAGTAEIKAVWALSLPAKCAPVTAGRFVADTVLQMIEERD"
BASE COUNT          435 a          967 c          836 g          454 t
ORIGIN      
        1 ggctgctttt gcccgcgccg gacgggccgt cgattgcgat ctggatcatc atcgttcccc
       61 tcacttttct tccaggatat gggtgccggc cgcatagccg gtggaaaaag cgatctgcag
      121 attataaccg ccggtgtagg cgtccacatc cagcagctcg cccgcaaaat gcaggttttt
      181 gacgagcttt gattccatgg tgcgggggct gacctcacgg acggacaccc cacctgccgt
      241 gaccaccgcc cgctcgatgg cgtccttgcc ggagatgggc agctcaaacc ccttgagcag
      301 gccggtcagc ctgcgccgct cctcccgggt gacctggttc acctttttgc ccggcggcac
      361 gccccagcgg gccagcatca ccggcaccat gcccgcgggc agcaggcccg aaaggctgtt
      421 gcccgcgtcc cggttggcaa acttttcaaa gtcccggttc accctggcat acagcgtcgg
      481 ctcatccagc gccggcttga ggtcgatcac cgcgcgcacg ccccgcaccg cgtccccgcg
      541 cagataagcg ctggctgaga gcaccagcgg ccccgagatg ccgaaatggg tgaacagcat
      601 ctccccctgt tccgtgtaaa gcggcttgtc cccctccaac agggtcagcc gcacgttgcg
      661 cagggagaga cccatcatgg cccggcactc ctgccgggcc gggccgccga cggcgagggc
      721 caccaaactg gcggtggtgg gcaccacagt gtgccccagc gcctgcgcca tgcggtagcc
      781 ggcgccggtg gagccggtgc ccggataaga gcggccgccg gtggcgagga tgacgccgtc
      841 cgccggcagc gacctgccat cggccagccg cacccccacc gccgcgccgt ccgcggcttc
      901 caccgccgca gcctcggcaa agagcacctt gcagccccgc gcatagcgcg cgagggcgtc
      961 ggcgacatcc gccgcccggt cgctgaccgg aaagacccgc cgcccgcgct cggtcttcag
     1021 cggcacgccc agggcttcca tcagggccat cgtgtccgca ggcgtaaagg cgttgagagc
     1081 ggaatagaga aatttggggt tgccccgcac atttttgagc acagccgccg ggtcgcagtc
     1141 attggtgacg ttgcagcgcc ccttgcctgt gaccaggatc tttttgcagg tgcgctccat
     1201 atgctcgacc acggtcacct ccaggccgcg ccgcgccgcg gtgcccgcgg cgagcatacc
     1261 ggccgcgccg ccgcccacca caacgatctt tttcaggcgt cccgccccct ttgcacagaa
     1321 gaaatgccgg acaccaggcc cggcattttt taatttttgc ttattgtttt atttatacca
     1381 caaacgcttt gaaatggcaa tatttttgcg cggtgcggcg tctgtccctc tctcaagccc
     1441 aggctgggag acgtccgcgc ggggctggac ccacgcggcc cgccccagcc cacaggcctc
     1501 aaaaaaagat cggctgcacc tggcggcccc gcagggcgga ttccaccgtg gcgggcagct
     1561 ggctgaagtc ggccaccagc gagctgggct ttttcagcca gtcgtcctgc tccagcggca
     1621 caaaataata atttttggta ttgagcaacc gccccaggtt ctgggcgctg gcggagaggc
     1681 tgtcgttgga cgccagcgcc agcaccaccg gccggccgca gcgcagatgg ctcttgcagg
     1741 ccatcgtgat cggggtatcg gtgatgccgc aggccagctt gctcagcgtg ttgccggtgc
     1801 agggcgagac caccagcagg tcgcagagct ttttcggccc caccggctcc gcggcctgga
     1861 tggtgtcgat gatctcgtgc ccggtgatct ccaccaggcg gcggcggaac tccgccgcgg
     1921 tgccgaagcg ggtgtccagg ctggccgcgt tgaacgacat ggcggggatc acctccgccc
     1981 cctggcgcac cagctcctcc agcgctttca ggctgtgctc aaaattgcag aagctgccgc
     2041 tcagcccata aatgatcgtg gtattcttca tggtcaatcc ctctcctcta tcatctgtaa
     2101 gaccgtgtcg gcaacaaacc gcccggcggt caccggcgcg catttggcgg gcaggctgag
     2161 cgcccacacc gccttgatct cggccgtgcc cgcggccgcc aggtcgatgc cgcccggcgc
     2221 agaggcgaga tcgagcaaaa aggcgccgct ccccatcgtg ccgatcatct cgcggtcgat
     2281 cagcggcgca ggcaccgtgt tgacgatgac gtcaaagccg cgcaccgtct cccgcagggc
     2341 gtcgatggtc tgggtgcggt agcccagggc cgaggccagc gcccggtccc gcggcttgcg
     2401 cgccgccacc gtcaccagcg ccccaaaccc cgtcagccgc tggcagagca gctttgcgat
     2461 gcgcccaaag ccggtgatga gcacccggct gttccacagt gtcgcctgcc gctcccgcag
     2521 caggatctcc accgcgccct cgcaggtggg gatggcgttc atcaccgcca gctcctcccg
     2581 ctgcaggtag tcgtccagct cgacgcccgc ggcctgggcc accgcggcgg cctcgccgct
     2641 gaccttgccc gcataagcga ttgccccggg ctttgccgcc cgcagcagcg cg
//
LOCUS       DBMDYA010000403         2692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8289_length_2692_cov_1.297954, whole genome shotgun sequence.
ACCESSION   DBMDYA010000403 DBMDYA010000000
VERSION     DBMDYA010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8289_length_2692_cov_1.297954"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="ACZPFK_09730"
     CDS             <1..1008
                     /locus_tag="ACZPFK_09730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406635.1"
                     /translation="TNPCTRFVNGVVYAAVGLVGALSVIGGRLSVGGLSSFLSYANQY
                     TKPFNEISGVAAEFQAALASAARIFQVLDEASEPPEAPGARALRDCRGRVEAEHVDFS
                     YRPDAPLIQDLNVWAKPGQHIALVGPTGCGKTTIINLLMRFYDVDNGVIRVDGNEIRE
                     LTRGSLRGCYGMVLQDTWLFSGTVRENIAYGRPDAPEDEIVAAAKAAHAHSFIKRLPQ
                     GYDTPVAENGGNLSQGQRQLLCIARIMLTDPPMLILDEATSSIDTRTEAKIQRAFDEM
                     MTGRTSFVVAHRLSTIRRADCILVMKAGRIIEKGTHEELLAENGFYAQLYNSQFAPSE
                     G"
     gene            complement(1826..>2692)
                     /locus_tag="ACZPFK_09735"
     CDS             complement(1826..>2692)
                     /locus_tag="ACZPFK_09735"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1406636.1"
                     /translation="DLSLKLAGGVPMCYDDYRDGTLTLKLYDAALGFDVSALQNEFVA
                     AAAAEPLDPEKDGGEGVKLTLEIAPGSHLWGYDVTYADGATTLYLQKAPALGTEPGKP
                     LAGVTVLLDAGHGGSDTGAAGIGGATGTAEKDVNLALTLAARDRLQQLGATVLMTRTD
                     DSFPTLQERWAMEQAQRPDFFIALHHNSTGLTRDTAGAKGLEVYYFERGSGSFARNLM
                     DAVSAATGREADEPKWNYFYVNRVTFAPSVLFEFGYLVNPDEFEDCVSEQGIRAAANG
                     VAEGVLRSIPVA"
BASE COUNT          411 a          949 c          881 g          451 t
ORIGIN      
        1 accaacccct gcacccgctt cgtcaacggg gtggtctatg cggcggtggg gctggtgggc
       61 gccctgtcgg tgatcggcgg ccgcctgtcg gtgggcgggc tgtccagctt tctcagctat
      121 gccaaccagt acaccaagcc ctttaacgag atctcgggcg tggcggcgga gtttcaggcc
      181 gcgctggcca gcgcggcgcg catctttcag gtgctggacg aggcgtccga gccgccggag
      241 gcccccgggg ccagggccct gcgcgactgc cgcggccggg tggaggcgga gcatgtggac
      301 ttttcctatc gcccggacgc gccgctcatc caggacctga acgtctgggc gaagccgggc
      361 cagcacatcg cgctggtggg ccccaccggc tgcggcaaga ccaccatcat caatctgctg
      421 atgcgctttt acgatgtgga caacggcgtg atccgggtgg acggcaatga gatccgggag
      481 ctgacgcggg gcagcctgcg cggctgctac ggcatggtgc tgcaggatac ctggctgttc
      541 tcgggcaccg tgcgcgagaa catcgcctac ggccgccccg acgcgcccga ggatgagatc
      601 gtcgccgccg ccaaggcggc gcacgcccac agctttatca agcggctgcc ccagggatat
      661 gacaccccgg tggcggaaaa cggcggcaat ctgtcccagg ggcaaaggca gctgctctgc
      721 atcgcccgca tcatgctcac cgacccgccg atgctgatcc tggacgaggc gaccagcagc
      781 atcgacaccc gcaccgaggc caagatccag cgcgctttcg acgagatgat gaccgggcgc
      841 accagctttg tggtggcgca ccggctgtcc accatccgcc gggccgactg catcctggtg
      901 atgaaagccg ggcgcatcat cgaaaagggc acccacgagg agctgttggc cgaaaacggt
      961 ttttacgccc agctctacaa cagccaattc gcccccagcg agggatagga cgggagaggg
     1021 cgcggcccgg cggcaagctg cggccgggaa gagccgcgcc gcctctgcga ggggcggacg
     1081 gcacaaccgc cccgctttcg gcgcgttact ccggccacat ccacgcgcct caagcgcctt
     1141 tttcagagag ggcgcgagaa agggtgaaag gagtgggccc gccgggccat gttttcctct
     1201 tctctgccct ctttgcccgc tgcgcctccg gctttcggcg agagccgttt cccccgccgg
     1261 gagaagtaaa aattgcgtcg gcgcagctcc gcccgcaagc agcctcccca cgcactccgc
     1321 ccaggtggtt tgggtgcccc ccatgaggca gctccgcccg caagcagtca tccccatgct
     1381 ggctcaggcg gaccgcttgc ggcacaattg ccccgctttc agtgcgccgc cagagccaaa
     1441 ggcgcgcgcg tgccagggcc ctttcggcga tgctgcccgc caaaggccgt ctgaaagggc
     1501 ttccgatgcg gggccgggga tttttaggga cgcttgtgac agcccgtcgc tccctcgctg
     1561 cgcttttggg gcctttccgc cgcgcccttg ccccgctttc accgcgccat cctggttgcc
     1621 gacggcggga tgcgtgccga cccggatgcg cgggagcgct tctccgcctt tctcttgcct
     1681 tgtgggaaat aaaataaaaa gatcagcctc ggccgggcgc cgaggctgat cttttttgtt
     1741 ttttgctctt tctgccgccc catcgcctgc cgtcgccccg cagcgcagcg tagcaggagg
     1801 cgcgtccccg ctgtcccggc cgcgctcagg ccaccgggat gctgcgcagc acgccctcgg
     1861 ccaccccgtt cgccgccgcc cggatgccct gctcgctgac gcagtcctca aattcgtccg
     1921 ggttgaccag atagccgaat tcaaacagca ccgagggcgc aaaggtcacc cggttgacgt
     1981 agaaatagtt ccacttcggc tcgtcggcct cccgcccggt ggccgcggag acggcgtcca
     2041 tcaggttgcg ggcaaagctg ccgctgccgc gctcaaaata gtagacctcc agcccctttg
     2101 ccccggcggt gtccctggtg aggccggtgc tgttgtggtg cagcgcgatg aaaaagtccg
     2161 gccgctgagc ctgctccatg gcccagcgct cctgcagggt gggaaacgag tcgtcggtgc
     2221 gggtcatcag caccgtcgcg cccagctgct gcagccggtc gcgggcggcc agggtcaggg
     2281 ccaggttgac gtccttttcc gccgtgccgg tggcgccgcc gatgcccgcc gcgccggtgt
     2341 cgctgccgcc gtggcccgcg tccagcagca cggtcacccc cgccagcggc ttgcccggct
     2401 cggtgcccag cgccggggcc ttttgcaggt acagggtggt ggcgccgtcc gcgtaggtca
     2461 cgtcgtagcc ccagagatgg ctgcccggcg cgatctccag cgtcagcttg accccctcgc
     2521 cgccgtcctt ctccgggtcc agcggctcgg cggcggccgc ggccacaaac tcgttttgca
     2581 gcgcgctcac gtcaaagccc aaagcagcgt cgtaaagctt cagggtcagg gtcccgtccc
     2641 ggtaatcgtc gtagcacatg ggcacgccgc ccgccaactt cagcgacagg tc
//
LOCUS       DBMDYA010000404         2688 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8303_length_2688_cov_1.178972, whole genome shotgun sequence.
ACCESSION   DBMDYA010000404 DBMDYA010000000
VERSION     DBMDYA010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8303_length_2688_cov_1.178972"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="ACZPFK_09740"
     CDS             <1..791
                     /locus_tag="ACZPFK_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR1406637.1"
                     /translation="VFPELFLPGYPYGMTFGFTVGSRSADGRLDWKRYYDNSILVPGP
                     ETELLARAAKTAGAYLSIGVSERDERTATLYNTNLIFSPEGALVSVHRKLKPTGAERV
                     VWGDAQQDYFPVVDTPWGAMGSLICWESYMPLARVALYEKGVSLYISPNTNDNEEWQA
                     TIRHIAIEGHCYFINSDMFFTRDTYPADLHCPEEIARLPEIVCRGGSCIVDPYGHYVT
                     QPVWDKEAIIRAELELDRVPASRMEFDACGHYSRPDVLELRVHE"
     gene            complement(928..2025)
                     /locus_tag="ACZPFK_09745"
     CDS             complement(928..2025)
                     /locus_tag="ACZPFK_09745"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR1406638.1"
                     /translation="MSERELKLEEALAAFEFGGAVRHAERFGEGHINDTFAVTVQDGG
                     RWILQRLNTAIFKRPQEVMENILGVTRHLRGKIEARGGDPERETLNVLLTRGGGPLFT
                     DSAGGVWRCYRFIEGTVCLQAARSGEDFCNAARTFGQFQQLLSDYPAATLHETIPHFH
                     DTPDRYRNFEAAVAADKMGRAAEVEREIAFVRARKEDCRVLADLLAAGKLPLRVTHND
                     TKLNNILIDVVTGEGLCVIDLDTVMPGLSAHDYGDSIRFGASTAAEDEPDLSRVHFSL
                     PLFEAYTQGYLASAGEALTGLEKQTLPWGAKLMTLECGMRFLTDYLEGDTYFKTHRPG
                     QNLDRTRTQLRLVEEMEQNWDEMAQIVKKYS"
     gene            complement(2022..>2688)
                     /locus_tag="ACZPFK_09750"
     CDS             complement(2022..>2688)
                     /locus_tag="ACZPFK_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1406639.1"
                     /translation="YAVPEGRVKPWGTAHAVLAARRLVDGPFAVINADDYYGREAFEK
                     IYDYLLNHPDGDRYAYTMVGYHLRNTLTENGHVARGVCRVSGQGLLEQVTEHTKIFKD
                     GEDAKYTEDDGATWRPLAGETTVSMNMWGFTASFLKEAEQRFPAFLDRALAKDPQKGE
                     YFLPSVVSALIGEGKAEVKVLTSRDKWYGVTYKEDKPVVVAAIRAMIGAGVYPERLWG
                     NEA"
BASE COUNT          430 a          929 c          840 g          489 t
ORIGIN      
        1 tcgtcttccc cgagctcttc ctccccggct acccctacgg tatgactttc ggcttcacgg
       61 tgggcagccg cagcgccgac ggccgcctgg actggaagcg gtattacgac aattccatcc
      121 tggtgccggg ccccgagacg gagctgctgg cgagggcggc caagacggcg ggcgcctatc
      181 tgagcatcgg cgtctcggag cgggacgagc gcaccgcgac cctctacaac accaatctga
      241 tcttttcccc cgagggggcg ctggtgtcgg tgcaccgcaa gctcaagccc accggcgccg
      301 agcgggtggt ctggggcgac gcccagcagg attatttccc ggtcgtggac accccctggg
      361 gcgcgatggg cagcctgatc tgctgggaga gctacatgcc gctggcccgg gtcgcgctgt
      421 acgaaaaagg cgtgtccctg tacatctcgc ccaacaccaa cgacaacgag gagtggcagg
      481 cgaccatccg gcacatcgcc atcgaggggc attgctactt tatcaacagc gacatgtttt
      541 tcacccgcga cacctatccc gcagacctgc actgccccga ggagatcgcc cgtctgcccg
      601 agatcgtctg ccgcggcggc agctgcatcg tggaccccta cggccactat gtgacccagc
      661 ccgtctggga caaagaggcg atcatccggg ccgagctgga gctggaccgg gtccccgcca
      721 gccgcatgga gttcgatgcc tgcgggcact actcccgccc ggatgtgctc gagctgcggg
      781 tacacgaata aaagcgcggt cagcgatgcc cgccgttttg ggccacccgc cgggcgggtg
      841 gccctttttt tgcaaaaaag gccggtgcaa gggcgcgttt tttgagttca acgtgccctg
      901 caccggcctt tccgtttcag gctggggtca gctgtacttt ttcacgattt gggccatctc
      961 atcccaattt tgctccatct cctccaccag gcgcagctgg gtgcgggtgc ggtccaggtt
     1021 ctgccccggc cggtgggtct tgaaataggt gtccccctcg agataatcgg tcaaaaagcg
     1081 catgccgcac tccagcgtca tcagctttgc gccccagggc agggtctgct tttccagccc
     1141 ggtcagcgcc tcgcccgcgc tggcgagata cccctgggta taggcctcaa agagcggcag
     1201 gctgaagtgt actcgcgaca gatccggctc gtcctcggcg gccgtggagg ccccaaagcg
     1261 gatggaatcg ccgtaatcgt gggcggacag gcccggcatc acggtgtcca ggtcgatgac
     1321 gcagagcccc tcgcccgtga ccacgtcgat gaggatattg ttcagcttgg tgtcgttgtg
     1381 ggtgacccgc agcggcagct tgccggcggc cagcaggtcc gccaacaccc ggcagtcctc
     1441 tttgcgggcg cggacaaagg cgatctcccg ctccacctcg gcggcgcgcc ccatcttgtc
     1501 ggccgccacg gcggcctcaa agttgcggta gcggtccggc gtgtcgtgaa aatgcgggat
     1561 ggtctcgtgc agcgtcgccg ccgggtagtc gctcagcagc tgctggaact gcccgaacgt
     1621 cctggccgcg ttgcaaaaat cctcgccgct gcgggccgcc tgcaggcaga ccgtgccctc
     1681 aataaagcgg tagcagcgcc agacgccccc cgcgctgtcg gtgaacagcg gcccgccgcc
     1741 gcgggtgagc agcacgttga gggtctcccg ctccgggtcg ccgccgcggg cctcgatctt
     1801 gccgcgcagg tgccgcgtca ccccgaggat attttccatg acctcctgcg gccgtttaaa
     1861 aatggcggtg ttcagccgct gcaggatcca gcggccgcca tcctgcaccg tgacggcaaa
     1921 ggtgtcgttg atatggccct cgccgaaccg ctccgcgtgc cgcacggcgc cgccaaactc
     1981 aaacgccgcc agcgcctcct ccagcttcag ctcccgttcg ctcatgcctc gttcccccaa
     2041 aggcgctcgg ggtagacgcc cgcgccgatc atggcgcgga tggccgccac caccaccggc
     2101 ttatcctctt tgtaggtgac cccgtaccac ttgtcgcggc tggtgagcac cttgacctcg
     2161 gccttgccct cgccgatcag cgcgctgacc acgctgggca aaaagtactc ccccttttgc
     2221 gggtcctttg ccagcgcccg gtcgagaaaa gcgggaaagc gctgctccgc ctctttcaaa
     2281 aagctggcgg tgaagcccca catattcatc gagacggtgg tctccccggc caggggccgc
     2341 caggtggcgc cgtcgtcctc ggtgtatttc gcgtcctcgc cgtccttgaa gatcttggtg
     2401 tgctcggtga cctgctccag cagcccctgg ccggacacgc ggcagacccc gcgggccaca
     2461 tgcccgtttt cggtcagggt gttgcgcagg tggtacccca ccatcgtgta ggcgtaccgg
     2521 tccccgtccg ggtggttgag cagataatcg taaatctttt caaacgcctc gcggccgtaa
     2581 tagtcgtcgg cattgatgac ggcaaagggg ccgtccacca gccgccgcgc cgccagcacc
     2641 gcgtgggcgg tgccccaggg cttgacgcgc ccctccggca ccgcgtag
//
LOCUS       DBMDYA010000405         2686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8311_length_2686_cov_1.720696, whole genome shotgun sequence.
ACCESSION   DBMDYA010000405 DBMDYA010000000
VERSION     DBMDYA010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_2686_cov_1.720696"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="ACZPFK_09755"
     CDS             <1..381
                     /locus_tag="ACZPFK_09755"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1406640.1"
                     /translation="VLEGDGASYILGVGINLLQTAAFFEAQGLDHAASLYSATGRRCL
                     PRDAALALDGALTRRLESFAGGGFAALAEEYRACCINLGRQVYTDTVRGVAAAIDDAG
                     RLVVRTAQGQEAVFTGEVTVHGIY"
     gene            complement(864..1292)
                     /locus_tag="ACZPFK_09760"
     CDS             complement(864..1292)
                     /locus_tag="ACZPFK_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1406641.1"
                     /translation="MFVRSAKEEDLPGLYALCCEQAARSLPQDIFEAVFRAAAKDTHR
                     RLVVALEGEEIVGYADMQLELLLSRCALAAVFKEFYVQESMRGRGIGTGMLISLSGQA
                     KSIGCGELEASCQRVNLKSQEFLERHGFVRSQHKFTRTVK"
     gene            complement(1428..2612)
                     /locus_tag="ACZPFK_09765"
     CDS             complement(1428..2612)
                     /locus_tag="ACZPFK_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR1406642.1"
                     /translation="MKKNKPVKIKRYRRSFSGSGDRNNLLRRILLWVLILAVIFGLGW
                     LVAKPGLDFASSLWYSYKNRDPGSSAVSQPGAGGASSSQPAETQPTPTPTPQTAGGGW
                     ASVALSAADTPEKAAALAQQLKAQGVENAVLTLKDDRGYLYYPSQVPLAETAVASTTV
                     DAAAIAQAFRDAGLTPVAGLCAFKDSIAPYADRSMAVKYGSEGDITWLDSSAELGGKP
                     WLNPNSAAAQQYIADLIAEVRDLGFEKVLLQNFQFPEGYSLEAASYGVLNGGKEQLLA
                     ELGKRYEAIDGVEVWFEFPSAAVSGENVTAYGASPASFGLQRVFVRLDAVAATNEAGE
                     NVSVPPAADAPALAALLEPLRAGGVTAFGLRAAAITDPAALAAANTAAKDTGFTSFFV
                     LP"
BASE COUNT          428 a          861 c          916 g          481 t
ORIGIN      
        1 gtgctcgagg gggatggggc gagctatatc ctgggggtgg gcatcaattt gctgcagaca
       61 gccgcttttt ttgaggcgca gggcctggac catgccgcct ccctctactc cgccaccggg
      121 cggcgctgcc tgccgcggga cgccgccctg gcgttggacg gcgcgctgac gcgcaggctg
      181 gagagcttcg ccgggggcgg ctttgcggcc ctggcggagg agtaccgcgc ctgctgcatt
      241 aatctcgggc ggcaggtcta caccgacacg gtgcgcggcg tcgcggccgc gatcgacgat
      301 gcgggccgcc tggtggtgcg caccgcccaa gggcaggagg ctgtctttac cggcgaggtg
      361 acggtgcacg gcatctattg agccaggttg gcttcgcagg cccggcgctc gggatgcagg
      421 tgaagtgtga ttccgggtat ttttaacccg cagaggcggt cacacaggcc gttttctaaa
      481 aaagagccgc agcggagcgg gggctatttt tttcggccat ccgtttcggt tgccgcgtag
      541 cggcgggggc cgaaccgtca gaataaaaga cagacgcagt gaccgcttta gcctgccgtt
      601 tgattgtcgc gcagcggaga aaccggcgcg agcgggacct ctttctgcga gataaaaaat
      661 agccgcagag cggctgtttt ttcggccatg cggcccgtcc tcaggccacg gccatagaaa
      721 caagctcttt tgttggccat ctgcttcggt tgccgcgaca gcggcgagaa gcgggaccat
      781 taagataagt tgttttttat tgcggagcaa taaaaaagac cggcgagatc gcgctttgcg
      841 cgtccgccgg tctttttttc gcgttatttg acggtgcggg tgaacttgtg ctggctgcgc
      901 acaaagccgt gccgctccaa aaattcctgg ctcttgaggt tgacgcgctg gcagctggcc
      961 tccagctcgc cgcagccgat ggattttgcc tggccggaga gggagatcag catgccggtg
     1021 ccgatcccgc gcccgcgcat ggattcctgt acataaaatt ctttgaagac cgccgccagc
     1081 gcacagcggg acagcaacag ctccagctgc atgtcggcgt atcccacgat ctcctcgccc
     1141 tccagggcca ccaccagccg ccggtgggtg tcctttgccg ccgcgcggaa cacggcctcg
     1201 aagatgtcct ggggcaggct gcgcgcggcc tgctcacagc agagcgcgta caggcccggc
     1261 aggtcctcct ccttggcgct gcgcacgaac ataagcgcgt ccctcctctt ttcggcaggc
     1321 cggcgccgcc gcacggcttt tccggtcatg ccctgcaccg cattttttcc gcccttttcc
     1381 gccctccggc ccagggccct gccccgcccg gtacggcccc caggggatca ggggagcacg
     1441 aagaagctgg taaagcccgt gtcctttgcc gccgtgttcg ccgcggccag cgcggcgggg
     1501 tcggtgatgg cggcagcgcg caggccgaac gcggtgacgc ccccggcccg caatggctcc
     1561 aaaagcgccg ccagggcagg cgcgtcggcg gcgggcggca cggagacgtt ctcccccgcc
     1621 tcgttggtgg cggcaacggc gtccagccgc acaaagactc gctgcagacc gaaagacgcc
     1681 ggggaggcgc cgtaggccgt gacgttttct ccgctgaccg cggcggacgg gaactcgaac
     1741 cagacctcca ccccgtcgat cgcctcgtac cgcttgccca gctcggccag cagctgctcc
     1801 ttgccgccgt tcaggacgcc gtagctggcc gcctccaggc tgtagccctc ggggaactgg
     1861 aagttctgga gcagcacctt ttcaaagccc aggtcccgca cctctgcgat gaggtccgcg
     1921 atatactgct gcgccgccgc cgagttgggg ttcagccagg gcttgccgcc cagctctgcg
     1981 gaggaatcca gccaggtgat atcgccctcg ctgccgtatt tcaccgccat cgagcgatcg
     2041 gcgtaggggg cgatgctgtc tttaaacgcg cacaggccgg ccacaggggt gagccccgca
     2101 tcgcggaacg cctgcgcgat cgccgccgcg tcaacggtgg tggaggcgac ggccgtctcg
     2161 gcgagcggca cctgggaggg atagtagagg tagccgcggt catctttgag ggtgagcacc
     2221 gcgttttcca ccccctgggc cttgagctgc tgggccaggg cggcggcttt ttcgggtgtg
     2281 tcggcagccg agagcgccac cgaggcccag ccgccgccgg cggtctgcgg cgtgggggtt
     2341 ggggtgggct gcgtctcggc gggctgggag gaggatgcgc cgcccgcccc cggctgcgag
     2401 acggcggagc tgcccgggtc gcggttttta tagctgtacc agagcgagga cgcaaagtcc
     2461 agccctggtt ttgccaccag ccagcccagg ccgaagatca ccgccaggat caggacccac
     2521 agcaggatgc gccgcagcag gttgttgcgg tcccctgagc cggaaaagct gcggcggtag
     2581 cgtttgattt tgacaggctt gttcttcttc aagcgaaagc ctcctcctac agagtgcaga
     2641 gcgccggacg gcaaatggtc agatggcggg cgtgtggccg gtgaac
//
LOCUS       DBMDYA010000406         2682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8329_length_2682_cov_1.366912, whole genome shotgun sequence.
ACCESSION   DBMDYA010000406 DBMDYA010000000
VERSION     DBMDYA010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8329_length_2682_cov_1.366912"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="ACZPFK_09770"
     CDS             <1..283
                     /locus_tag="ACZPFK_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1406643.1"
                     /translation="ANQLRLEGRERREALVETGRTRIRPILMTALTTILAMCTMALGV
                     GDGAEMTQPMAIVTIGGLTYATLLTLLVVPVMYDIFCRKDLKPIDIEEA"
     gene            287..916
                     /locus_tag="ACZPFK_09775"
     CDS             287..916
                     /locus_tag="ACZPFK_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1406644.1"
                     /translation="MVEKRPCTEKETALFAAVTRLAASGRELHSLKVSEIAAEAGIGK
                     GTVYEYFKSKEELIGAAMLYNLEQLFARAEETLAGAEGFREGFYACIGMVREALQRRL
                     SGVQMLVSELRRGELETYLGGRMEELEQYRSRMDALIARVVLLGEAEGLFAAADPWGY
                     RRFVFGAAVASSAMAGGVCLQQGEARQLDGYAYEMLLRALRGPQREEKT"
     gene            1226..2470
                     /locus_tag="ACZPFK_09780"
     CDS             1226..2470
                     /locus_tag="ACZPFK_09780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR1406645.1"
                     /translation="MKKIIKCGSLFTAADESVQKNMAVVVEGNRITAVLPADKADTAG
                     AEVLDLSDKFVMPGLIDAHLHTNISGEPSILHAFVDGSEGAFTITSIKNAQRNLMAGF
                     TSVRDEGGFRYTDVAVRDAINKGMIVGPRMMVSGEALTGTGGHADSHLAPYVGGFTSL
                     GKVVNSADEGRKAARLNLKYGADQIKIMATGGVMSFGDDPKCSELSFEEMKAIIDIAK
                     AHGRITSAHAHGSDGIKIATRAGITSIEHGMLMDDECVELMAQHGTYLVPTIIAAKAI
                     VDNGVSGGMAPWLVEKAQLVLSGHKENLRKCREAGVPIAFGTDAGTCYNYHGAQTVEF
                     GLMVEFGFTPAQTLIAATRTNATMMRWIDRLGTVEAGKLADLAAFDKSPLEDIEVMNS
                     VSFVMKDGVVCKKDGAPCVTLQ"
     gene            2486..>2682
                     /locus_tag="ACZPFK_09785"
     CDS             2486..>2682
                     /locus_tag="ACZPFK_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406646.1"
                     /translation="MKQILKCGHLFSTAAKGFVADQNVYVQDNKIVEVAGGPVKEGWE
                     VVDLSGKYVLPGLIDTHVHCNL"
BASE COUNT          497 a          850 c          932 g          403 t
ORIGIN      
        1 cgccaaccag ctgcgcctgg agggcaggga gcggcgggag gccctggtgg agaccgggcg
       61 cacccgcatc cgccccatcc tgatgacggc cctgacgacg atcctggcca tgtgcaccat
      121 ggcgctgggc gttggggacg gcgccgagat gacccagccc atggccatcg tcaccatcgg
      181 cggcctcacc tacgccacgc tgctcaccct gctggtggtg ccggtgatgt acgatatctt
      241 ctgccgcaaa gacctgaagc ccatcgacat cgaggaggcg tagagcgtgg tggaaaagag
      301 gccctgtacc gaaaaggaga ccgccctttt tgcggcggtg acgcggctgg ccgcctccgg
      361 gcgggagctg cacagcctga aggtgtctga gatcgcggcc gaggcgggca tcggcaaggg
      421 caccgtctac gagtatttca agagcaagga ggagctgatc ggcgccgcta tgctatacaa
      481 cctggagcag ctgtttgccc gcgccgagga gactctggcc ggggcggagg gcttccgcga
      541 ggggttctac gcctgcatcg ggatggtgcg cgaggcgctg cagcgccgcc tctccggcgt
      601 gcagatgctg gtcagcgagc tgcggcgcgg cgagctggag acctacctgg gcggccgcat
      661 ggaggagctg gagcagtacc gcagccgcat ggacgcgctc atcgcccggg tggtcctgct
      721 gggcgaggcg gagggcctgt tcgccgccgc cgacccctgg ggctaccggc gctttgtctt
      781 cggcgcggcg gtggcctcca gcgccatggc gggcggcgtc tgcctgcagc agggggaggc
      841 gcgccagctg gacggctacg cctacgagat gcttctgcgg gccctgcggg ggccgcaaag
      901 ggaggaaaag acctaggcgg cccccgcgcc gcccctcctg ccgcccaagg cggcgctgtg
      961 gggcggtccg gggggcaaag ggccggggcg cttgcccctc tttccgccaa aagccacgcc
     1021 ctgcgccgcc gggacggggg attttttgtg gcctttcacg attgcgcggt cggcccaagt
     1081 ttgatacaat gatagccaaa gttttgaaaa aaggggaggc gctgcaaaag ccgggtcaag
     1141 gcccggccga ctgcggcgcc tctctgcaca agactggcac acccggcggc gcggccgccg
     1201 cgggaagtta agggaggaaa gacggatgaa aaaaatcatc aagtgcggca gcctgttcac
     1261 cgccgccgac gagtcggtgc agaaaaacat ggccgtcgtc gtcgagggca accgcatcac
     1321 cgcagtgctg cccgccgaca aggcggacac ggccggggcg gaggtgctgg acctgtcgga
     1381 caagttcgtg atgccgggcc tcatcgacgc ccatctgcac accaatatct ccggcgagcc
     1441 cagcatcctg cacgcctttg tggacggcag cgagggcgcg ttcaccatca ccagcatcaa
     1501 aaacgcccag cgcaacctga tggcgggctt taccagcgtg cgggacgagg gcggcttccg
     1561 ctacaccgac gtggcggtgc gggacgccat caacaagggc atgatcgtgg gcccgcgcat
     1621 gatggtgtcc ggcgaggcgc tcaccggcac cggcggccat gcggactccc acctggcgcc
     1681 ctatgtgggc ggcttcacct ccctgggcaa ggtcgtcaac agcgccgacg agggccgcaa
     1741 ggccgcccgg ctcaacctga aatacggcgc cgaccagatc aagatcatgg ccaccggcgg
     1801 cgtgatgagc tttggcgacg accccaagtg cagcgagctg tcgtttgagg agatgaaagc
     1861 catcatcgac atcgccaagg cccacggccg catcacctcc gcccacgccc acggcagcga
     1921 cggcatcaag atcgccaccc gcgccggcat cacctccatc gagcacggca tgctgatgga
     1981 cgacgagtgc gtcgagctga tggcgcagca cggcacctat ctggtgccca ccatcatcgc
     2041 ggccaaggcc attgtggaca acggcgtcag cggcggcatg gccccctggc tggtggaaaa
     2101 ggcgcagctg gtcctctccg gccacaagga gaacctgcgc aagtgccgcg aggcgggcgt
     2161 gcccatcgct ttcggcaccg acgcgggcac ctgctacaat taccacggcg cccagaccgt
     2221 ggagttcggg ctgatggtgg agttcggctt cacgcccgcc cagaccctga tcgcggccac
     2281 ccgcaccaac gccaccatga tgaggtggat cgaccggctg ggcaccgtgg aggcgggcaa
     2341 gctggcggac ctggcggcgt ttgacaagag cccgctggag gatatcgaag tgatgaacag
     2401 cgtctccttt gtgatgaagg acggcgtggt ctgcaaaaag gacggcgcgc cctgcgtcac
     2461 cttgcagtaa agggggaaaa cagtgatgaa acagattttg aaatgcgggc atctgttttc
     2521 gaccgcggcc aagggctttg tggcggatca gaatgtctat gtgcaggaca ataagatcgt
     2581 cgaggtggcg ggcggcccgg tgaaagaggg ctgggaggtc gtcgacctgt cgggcaagta
     2641 tgtgctgccc ggcctgatcg acacccatgt gcactgcaac ct
//
LOCUS       DBMDYA010000407         2677 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8347_length_2677_cov_1.589286, whole genome shotgun sequence.
ACCESSION   DBMDYA010000407 DBMDYA010000000
VERSION     DBMDYA010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_2677_cov_1.589286"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="ACZPFK_09790"
     CDS             complement(<1..441)
                     /locus_tag="ACZPFK_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406647.1"
                     /translation="MTKTVKWSRYLWLSLLCFSFFLLELVSIFGIEMLFLRVDVWAYT
                     PDQRARHSLITAALWAAAMAALLLYARRRYGFPTRAGIRQKSSARDWAAALLCLAGCK
                     VMTFIDWQTLKIIGEARGKTAFQFCAQYLYYLLEVGLVCLILLWG"
     gene            complement(489..815)
                     /locus_tag="ACZPFK_09795"
     CDS             complement(489..815)
                     /locus_tag="ACZPFK_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR1406648.1"
                     /translation="MTFPQAVITVAMVVLGALITRFLPFVLFPASKPTPAYVRYLGQV
                     LPYAVTGLLVVYCLRSVSVLRAPFGLPELAALAVIAAAHLWKKNTLLSLLAGTATYML
                     MVQVVF"
     gene            complement(818..1516)
                     /locus_tag="ACZPFK_09800"
     CDS             complement(818..1516)
                     /locus_tag="ACZPFK_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1406649.1"
                     /translation="MKTQAFKAALPHCLPVCAGFLFIGMSYGVYMSSLGFSFVWPMVM
                     SVAIFAGSMEFLTANLLVLNFDPLNALLLALTVNARHLFYGISMLDKYRAAGKKKWYL
                     IYGMCDESFAINASAKPPEGVDQGWFMFFVTLLNQCAWVGGATLGGLVGGLIPFDTTG
                     IDFVLTALFVVIFLGQWQETKQHLPALTGVLCSVLALLIFGADSFILPAMILILLALT
                     ALRKKLEKKEGSAL"
     gene            complement(2061..>2677)
                     /locus_tag="ACZPFK_09805"
     CDS             complement(2061..>2677)
                     /locus_tag="ACZPFK_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1406650.1"
                     /translation="LGVEIDASHQYNTFHLMRVGSLLIGALLLAASVLVAVLLFRRIS
                     NPSYRDLANTDYLTGCKSRNAFEVDLGNLQSARRQGQALLVSIDLNGLKKVNDTAGHA
                     GGDELIRRAADLLRRALLPGMVLYRVGGDEFAILAETPGEDAEQRLRAGLRTVLDARG
                     GGVSLSMGAARFDPARDKDLLDTYRRADLEMYGQKRRFYGGGRA"
BASE COUNT          523 a          889 c          831 g          434 t
ORIGIN      
        1 cccccagagc aaaatcaggc agaccagccc cacctccagc agatagtaga ggtattgggc
       61 gcagaattgg aacgccgttt tgccgcgggc ctcgccgatg attttcagcg tctgccaatc
      121 gatgaaagtc atcaccttgc agcccgccag gcagagcagc gcggcggccc agtcgcgggc
      181 cgagcttttc tgccgtatcc ccgcccgggt gggaaagccg taccgtctgc gggcgtacag
      241 cagcagcgcg gccatggcgg ctgcccacag ggccgcggtg atcaggctgt gccgcgccct
      301 ctggtcgggg gtataggccc agacgtccac ccgcaaaaaa agcatttcaa tgccgaagat
      361 cgagaccagc tccagcagaa aaaaggaaaa gcagagcagg ctgagccaga gataacggct
      421 ccatttcacc gtttttgtca tgcgcttccc tctctttgga ccgtcatttt ttcagcatgg
      481 ggatccgctt aaaacaccac ctgcaccatc agcatatagg tggcggtgcc cgccagcagc
      541 gagagcaggg tgttcttttt ccacagatgg gccgccgcga tcaccgcgag ggcggccagc
      601 tcgggcaggc cgaaaggggc gcgcagcacc gagacgctgc gcaggcaata gaccaccagc
      661 aggccggtga cggcgtaggg cagcacctgc cccagatagc gcacataggc cggggtgggc
      721 ttgctggccg ggaagagcac aaacggcaga aaacgggtga tgagggcgcc cagcaccacc
      781 atggcgacgg tgattaccgc ctggggaaaa gtcatcgtca cagcgcgctc ccctcctttt
      841 tttccagttt tttgcgcagc gcggtcagcg ccagcaggat caggatcatg gccggcagga
      901 taaagctgtc cgccccgaaa atcagcagcg ccagcaccga gcacaacacc ccggtgaggg
      961 cgggcagatg ctgtttggtc tcctgccatt ggcccaaaaa gatgaccacg aaaagggcgg
     1021 tgagcacaaa atcaataccg gtggtgtcaa aggggatcag cccgcccacc aggccgccca
     1081 gtgtggcccc acccacccag gcgcactggt tgagcagggt gacaaaaaac atgaaccagc
     1141 cctggtccac cccctcgggc ggcttggctg aggcgttgat ggcaaagctc tcgtcgcaca
     1201 tgccgtagat cagataccac ttctttttgc ccgcggcgcg atatttgtcc agcatcgaga
     1261 tgccgtaaaa caggtgccgg gcattgacgg tgagcgccag cagcagcgcg ttcagcgggt
     1321 caaagttcag caccagcagg ttggcggtga gaaactccat cgaccccgca aagatggcga
     1381 cgctcatcac catcggccat acgaacgaaa agcccaagct ggacatgtag acgccgtagg
     1441 acatgccgat gaacaaaaag cccgcgcaga ccggcaggca gtgcggcagc gcggctttaa
     1501 aggcttgtgt tttcatcctt tctcttcgct tcctttcctc tctgctcctc gcgggcccgg
     1561 cccgcggggc catttcgttc cattatactt tggcgcggcg ccagcgtcaa gaaaggcgaa
     1621 aacacatccc ccgactccgt gcggcaggga aaggcgcggc ccgcatctca aaacaccggc
     1681 ccgccttttc caaggcgggc cgtcagaatg ggctaggggc aaaaagccgc ctcctttcgc
     1741 gtgcaggtgg ctttggcatc ccgcgggcaa aaggcaaaaa agcgcagtcc tccggcgcag
     1801 ttgcggctgg ggacgccgat ctctgccggc aaaagagccc gtccgccgcg ccgtcgccgc
     1861 ctgcacgtta gccgggcctg cggcgctccg cctccctgca acggtttttt ctcccgcggc
     1921 cggcaccgcg cagtttttgc ccggcggggc aaagccgcgt gtttcccgtt ttcaccgcgc
     1981 ctttgggccg cgcgcgctcg gccgggtccc caggggccgg caggaaaaag ttttctgccc
     2041 accgccgcgg ggcccgccgc tcaggcccgg ccgccgccgt aaaagcggcg cttttggccg
     2101 tacatctcca gatcggcgcg ccggtaggtg tccagcaggt ccttgtctct ggccgggtcg
     2161 aaccgcgccg cccccatcga cagcgacacc ccgccgcccc gggcgtccag cacggtccgc
     2221 aggcccgcgc gcagccgctg ttccgcgtcc tcgccgggcg tctccgccag aatggcgaac
     2281 tcatcgccgc ccacccggta aagcaccatg ccggggagga gggcccgccg cagcaggtcc
     2341 gccgcccggc gaatgagctc gtcgccgccc gcatggccgg ccgtgtcatt gacctttttc
     2401 aggccgttga ggtcgatgga gaccaacaga gcctgcccct gccggcgcgc gctctgcaga
     2461 ttgcccaggt cgacctcgaa agcattgcgg cttttgcagc cggtcagata atcggtgttg
     2521 gcgaggtctc ggtagctggg gtttgagatg cggcggaaca gcagcaccgc caccagcacc
     2581 gaggcggcca gcagcagcgc gccgatgagc agcgagccca cccgcatcag gtggaaagtg
     2641 ttgtactggt gggaggcgtc gatctccacc cccagca
//
LOCUS       DBMDYA010000408         2672 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8362_length_2672_cov_1.189420, whole genome shotgun sequence.
ACCESSION   DBMDYA010000408 DBMDYA010000000
VERSION     DBMDYA010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_2672_cov_1.189420"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="ACZPFK_09810"
     CDS             <1..623
                     /locus_tag="ACZPFK_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406651.1"
                     /translation="LVDPKQVKTQKGQKQLKDYSVKQMLKTPQYYMMFSVVALATPAV
                     LLFSPIIVELGQQRGLDERTALLSIIIGSIGSAAGRLLMPLLSDKIGRRATDMILFGA
                     LAVLSFGFIFAGGWWVIAAYTALTFCYSGEAAVIPAIGTDLYGYKNAGVNYGFLALGM
                     SLGSVAFSLLANSFGGPLPRHITAIAAAAVGFAVLIFLKPTQGKRL"
     gene            982..1200
                     /locus_tag="ACZPFK_09815"
     CDS             982..1200
                     /locus_tag="ACZPFK_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406652.1"
                     /translation="MLSYRLLWEVMRQRKITTYVLINTYGISSRTVNNLKHDKSITMY
                     TLEKLCSILKCTPNDVVEFLPNPDELKE"
     gene            1465..1830
                     /locus_tag="ACZPFK_09820"
     CDS             1465..1830
                     /locus_tag="ACZPFK_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR1406653.1"
                     /translation="MDHNVIQMEHAMWEAALHRDADAFKKLVSPDAVMVYGGCRCLGR
                     EYADLLADFRIDGYSITHMEVIDASDRQVTLHYVIRTRSEDAGAQDLAGLFHVVSLWK
                     RKNETWKLIFNMDSRIMER"
     gene            1907..2671
                     /locus_tag="ACZPFK_09825"
     CDS             1907..2671
                     /locus_tag="ACZPFK_09825"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1406654.1"
                     /translation="MKLKSVLLEELSWREIGEAMEGGCRTVLVCAASNEQHGPHLAEN
                     TDYIFGLEMCRAVAEKMGDALVAPVIRPGLSQHHMRHPGSLTLRPETFRMLVEDYVDC
                     YVRHGFERIVLLASHGTNFAPLAALAPELQKKYPRTKIVCGMSLEDFMALYDEGDRMF
                     GLEPGACGGHACCFETSVILYLHPEYVQMSRAERGYMEGNTPAFAKSLFENGMMGVSP
                     CGIVGDATPADREKGRAFFELFCRRACACVQAALAQ"
BASE COUNT          525 a          751 c          863 g          533 t
ORIGIN      
        1 tgctggtgga cccaaagcag gtcaagacgc agaaaggcca aaaacagctc aaggactaca
       61 gcgtcaagca gatgctcaag accccccagt actatatgat gttctcggtg gtggcgctgg
      121 ctaccccggc ggtgctgctc ttttccccca tcatcgtgga gctggggcag cagcgggggc
      181 tggacgagcg cacggccctg ctctccatca tcatcggcag catcggcagc gccgccgggc
      241 ggctgctgat gccgctgctg tcggacaaga tcggccgccg cgccaccgat atgatcctgt
      301 tcggcgcgct ggcggtgctc tctttcggct ttatcttcgc cggcggctgg tgggtgatcg
      361 ccgcctatac ggcgctgacg ttctgctatt ccggcgaggc ggcggtgatc ccagccatcg
      421 gcaccgacct gtacggctac aaaaacgccg gggtcaacta cggctttctc gccctgggca
      481 tgagcctggg cagcgtggcc ttttcgctgc tggcaaacag cttcggcggc ccgctgcccc
      541 gccacatcac ggccattgcg gcggcggccg tcggctttgc ggtgctgatt tttttgaagc
      601 ccacccaggg caagcggctg taaaaccgcc ctgcggcccc tcccctctcc cgccctctcc
      661 catgccccgg ccccggcaaa ggccggggct ttttgcgtgg cggcagtgtt gcgggaaagg
      721 ggggagagca gggcggaacc ggggcgcggg ggcatccgca tcccgcttgc gctgtccggc
      781 aatgaccggc caggcaaacg ccggcgccag cgccgggcga acagacggga cgccccgcag
      841 aggccccgtc ccctctgcgc tttctctttt agggagcggt ggaaacgtca aataaattat
      901 agttatattt ttcagtattt caaatcttgg ctttgttcac gataatcaga gtagattgtg
      961 ttgaaaaggg gggaagcgac gttgctgagc tatcggctgc tttgggaagt gatgcgccag
     1021 cgcaaaatca ccacctatgt cttgatcaat acttatggaa taagctctcg gacagtcaac
     1081 aatttaaagc atgataaaag tattacgatg tatacgcttg aaaaactgtg ttcgatttta
     1141 aagtgcaccc caaatgatgt cgtggaattt ttgccgaatc ctgacgagct gaaagagtag
     1201 gggacggtcc tcgggccgcg gccaagaaat aggtcgtttt tattgcgaag caataacaac
     1261 agccgttttg cggctgttgt gcggccgtcc gctccggttg ccgcgacggc ggcgaaaaac
     1321 ggggcagccg ggataaaaag cagaaaggct gctggacatt tcgatgcgct gccggctgcc
     1381 gttaggacga aaagcttctg aggcgcgcgt gcgtttcagg cgcggggcgg acattcagag
     1441 ggcggaacag agccggaggg ggatatggat cacaacgtca tccagatgga acacgctatg
     1501 tgggaagcgg cgctgcacag ggatgcggac gcgtttaaaa agctggtgtc gcccgatgcc
     1561 gtgatggtat acggcggctg ccggtgcctg gggcgggagt acgcggatct cctggcggat
     1621 ttccggatag atggatattc gatcacccat atggaggtga tcgacgcaag cgaccgccaa
     1681 gtgacgttac actatgtcat ccgcacccgg tctgaggacg cgggcgcgca ggatctggcg
     1741 gggctttttc atgttgtatc tctgtggaaa agaaaaaacg aaacctggaa gctgatcttc
     1801 aatatggatt caagaataat ggaaagataa tgtgaagctt cgacgcgcct tgttgcataa
     1861 ggcggatttt ttctcacaga gacaaatatg gcggggaggg tgcgctatga aactgaagag
     1921 cgttttgctg gaggagctgt cctggcggga gatcggggag gcgatggagg gcggctgccg
     1981 caccgtgctg gtctgcgccg cgtccaacga gcagcacggc ccgcatctgg ccgagaacac
     2041 cgattatatc ttcggcctgg agatgtgccg cgcggtggcg gagaaaatgg gggacgccct
     2101 ggtggccccg gtcatccggc ccggcctgtc ccagcaccat atgcgccacc cgggcagcct
     2161 caccctgcgg ccggagacgt tccgcatgct ggtggaggat tatgtggact gttatgtgcg
     2221 ccacggcttt gagcgcattg tgctgctggc ctcgcacggc accaactttg ccccgctggc
     2281 tgccctggcg cccgagctgc aaaaaaaata cccccggaca aagatcgtct gcggcatgag
     2341 tttggaggat tttatggccc tttacgacga gggcgaccgg atgttcgggc tggagccggg
     2401 cgcctgcggc ggccacgcct gctgctttga gacctcggtc atcctctatc tgcacccgga
     2461 gtatgtgcag atgtcccgcg ccgagcgcgg ctacatggag ggcaacaccc cggcgtttgc
     2521 caaaagcctg tttgaaaacg gcatgatggg ggtctcgccc tgcggcatcg tgggcgacgc
     2581 caccccggcc gaccgggaaa aaggcagagc cttttttgag ctgttctgcc gccgcgcctg
     2641 cgcctgcgtg caggccgcgc tggcgcagtg ag
//
LOCUS       DBMDYA010000409         2667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8380_length_2667_cov_1.574435, whole genome shotgun sequence.
ACCESSION   DBMDYA010000409 DBMDYA010000000
VERSION     DBMDYA010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8380_length_2667_cov_1.574435"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            115..690
                     /locus_tag="ACZPFK_09830"
     CDS             115..690
                     /locus_tag="ACZPFK_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406655.1"
                     /translation="MSMLVSYSETPLVLDAVSALKVTHYYGQSALDDVYAYFRAFACG
                     AGLVFSLTSFERRPPRESRVAAALCLDPKAEDYLFFSTNEALESECALYRQQGAGPDL
                     RVRPLALPRPPARFGGSDEQGFYWGCELLLDEAFLRQAFGVCLRPGSVFTGNVYKYAA
                     GEAAFGAAFPSPHGAGLPCRAGFGEFVVVPY"
     gene            752..895
                     /locus_tag="ACZPFK_09835"
     CDS             752..895
                     /locus_tag="ACZPFK_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406656.1"
                     /translation="MLKGLLKLFLMLAGLFAAVTGLGYLSRKNADQYIEIYGDEDDEE
                     DAF"
     gene            complement(994..1764)
                     /locus_tag="ACZPFK_09840"
     CDS             complement(994..1764)
                     /locus_tag="ACZPFK_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1406657.1"
                     /translation="MLIKLAVCIVAGLGAGIGTGLAGLSAAAVISPMLITFLGFPAYE
                     AVGISLASDVLASAVSAYTYGKHKNLDVKNGLVMLASVLVFTLVGSYAASRMPNAAMG
                     GFSVFMTLLLGVKFILRPVMTAKGGRAQKTLARRVAESVLCGMAIGFICGFIGAGGGM
                     MLLLILTSVLGYELKTAVGTSVFIMAFTALTGAVSHMAIGGAPDPFALVICVLAPLAG
                     ARGSALFANRADPKTLNRFTGVVLTVLGAAMVIVRYLF"
     gene            complement(1754..2197)
                     /locus_tag="ACZPFK_09845"
     CDS             complement(1754..2197)
                     /locus_tag="ACZPFK_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1406658.1"
                     /translation="MNERQNLLLHGQKLKRAYDSVFRPVMARHGLTRNEVDVLLFLAN
                     NPGRDTAVELVELRGLAKSQVCRSVEALQARGMLVGEQDPKDRRRVHLALTGRAAAPV
                     REAQQAQRLFIDLLYRGVTEEERAILARVFDRIGKNVQEVLEHAD"
     gene            2378..>2667
                     /locus_tag="ACZPFK_09850"
     CDS             2378..>2667
                     /locus_tag="ACZPFK_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406659.1"
                     /translation="MKGWRAAAAVLLAVACLWQWTRGAERTAHIPPQTPRCDLAPALE
                     GGAPAGGSERARQLFLQTGLGPAGVSAVLGEGGAPALLRMQAQLYAPALWQCR"
BASE COUNT          432 a          921 c          869 g          445 t
ORIGIN      
        1 actgaaaaca acgacggcgg cggcccgccg gcccgcgccg cgcgcggccc catgccgcgg
       61 cccccggggc tctctcagcc cgcgcgcagc ctatctcagc gaggtgatcc ggttttgtcg
      121 atgcttgtca gctacagtga aacgccgctg gtcctggacg cggtgagcgc cctcaaggtg
      181 acccattact atgggcagag cgcgctggat gacgtatacg cctattttcg cgcctttgcc
      241 tgcggcgcgg ggctggtgtt cagcctgacc agctttgagc ggcgcccgcc ccgcgagagc
      301 cgggtcgccg ccgccctctg cctggaccca aaggccgagg actacctgtt cttcagcacc
      361 aacgaggcgc tggagagtga atgcgccctc taccgccagc agggggcggg gccggatctg
      421 cgggtgcggc cgctggccct gccgcgcccc cctgcccgct ttggcggctc ggacgagcag
      481 ggcttttact ggggctgtga gctgctgctg gacgaggcgt ttttgcggca ggcctttggg
      541 gtgtgcctgc gcccgggcag cgtctttacc ggcaacgtct acaaatacgc cgccggtgag
      601 gccgcattcg gggccgcgtt cccctctccg cacggcgcgg ggctgccctg cagggctggt
      661 tttggggaat ttgtagtagt tccgtactga gcttcgttac tgttatgagt atatttttcg
      721 tctcgatcaa aaggaatggg ggaactgggg aatgttgaaa ggattgctga agctttttct
      781 gatgctggcc ggcctcttcg ccgcagtcac ggggctgggc tatctcagcc gcaaaaacgc
      841 ggaccagtac atcgagatct acggcgacga ggacgatgag gaggacgcgt tctgacccct
      901 cttccgcctt tcccgtctgc tgcaaaaccg gcgcttaccg ctcttgtggg cggcaagcgc
      961 tgtttttttg ccgcgcttcg cgcccccgcg cgctcaaaac aggtagcgca cgatcaccat
     1021 cgccgccccc agcacggtca gcacgacgcc ggtgaagcgg ttcagcgttt tggggtcggc
     1081 ccggttggcg aacagggccg agccgcgcgc cccggccagg ggggccagca cgcagatcac
     1141 cagggcaaag gggtccggcg cgccgccgat ggccatgtgg gacaccgcgc cggtgagggc
     1201 cgtgaacgcc atgataaaga cgctggtgcc caccgccgtc ttcagctcgt agcccagcac
     1261 gctggtaagg atgagcagca gcatcatgcc gccccccgcg ccgatgaaac cgcagataaa
     1321 gccgatggcc atgccgcaga gaacggattc ggccacccgg cgcgccaggg ttttctgcgc
     1381 gcgtcccccc ttggcggtca tgaccgggcg caggatgaat ttgacgccca gcagcagggt
     1441 cataaagacc gaaaatccgc ccatggcggc gttgggcatg cgcgaggccg catagctgcc
     1501 caccagggtg aagaccaata ccgaggccag catcaccagg ccgtttttga cgtccaggtt
     1561 tttgtgcttg ccgtaggtgt aggccgagac cgccgaggcc agcacgtccg aggccagcga
     1621 gatgcccacc gcctcatagg ccggaaaccc caaaaacgtg atcagcatgg gggagatcac
     1681 cgccgcggcc gaaaggcccg ccagcccggt gccgatcccc gcccccagcc cggccacgat
     1741 gcagacggcc agtttaatca gcatgctcca gcacctcctg gacgtttttc ccgatccggt
     1801 cgaacacccg cgccagaatg gcgcgctcct cttcggtcac gccccggtag agcaggtcga
     1861 taaaaaggcg ctgcgcctgc tgcgcctccc gcaccggcgc cgccgcccgc ccggtcaggg
     1921 ccaggtggac ccggcggcgg tccttggggt cctgctcccc caccagcatc ccccgcgcct
     1981 gcagcgcctc caccgacctg cagacctgtg atttcgccag cccccgcagc tccaccagct
     2041 ccaccgccgt gtcgcgcccg gggttgttgg ccagaaacag cagcacgtcc acctcgttgc
     2101 gggtcagccc atgccgggcc atgaccggcc ggaaaacgct gtcataggcc cgcttcagct
     2161 tttgcccgtg cagcagcaga ttttgacgct cgttcatccc cgcgcccctc tctcttttca
     2221 aacggatagt tctaaaaaga actttttaag tttaccccac cgcccccttt cctgtcaagc
     2281 gggcgctcat aatcccgtcc cggcggggcc acactagagg gcaggtgcgg gcagggcgcg
     2341 aaaagcgccg gccgcagccg ggaaagaggg cgctgggatg aaaggatggc gggccgccgc
     2401 ggccgttctg ctggccgtgg cctgtctctg gcaatggacc cgcggcgcgg agcggaccgc
     2461 ccatatcccg ccgcagacgc cccgctgcga cctggccccg gcgctggagg ggggcgcccc
     2521 tgcgggcggg agcgagaggg cgcggcagct ctttttgcag accggcctcg ggcccgcggg
     2581 cgtgagcgcc gtactgggag agggcggcgc gcccgccctg ctgcggatgc aggcgcagct
     2641 ctacgccccc gccctttggc agtgccg
//
LOCUS       DBMDYA010000410         2661 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8404_length_2661_cov_1.333984, whole genome shotgun sequence.
ACCESSION   DBMDYA010000410 DBMDYA010000000
VERSION     DBMDYA010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8404_length_2661_cov_1.333984"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="ACZPFK_09855"
     CDS             complement(<1..513)
                     /locus_tag="ACZPFK_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1406660.1"
                     /translation="MPILTVENVTKRFGGFEALKGVSLTVERGEAVAIIGSSGSGKST
                     LLRCVNNLERVSGGRIVLDGDTLVETVDGAVKYPPEKELRRICMKTGMVFQQFNLFPH
                     LTCLENITIAPIQILKQNRADAIANADRLLRLVGLENKADSYPAMLSGGQQQRVAIAR
                     ALAIDPQIMLF"
     gene            complement(517..1176)
                     /locus_tag="ACZPFK_09860"
     CDS             complement(517..1176)
                     /locus_tag="ACZPFK_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1406661.1"
                     /translation="MDLSYIALLMPALMQGLWQTLKIFALTLIFSLPLGLVFTLGSIS
                     RVLPLRWLARAYIWIFRGTPLMLQLFFVYYGLPFLGLTFDRFPAAVLTFVLNYAAYFA
                     EIYRGGIQSIDRGQHEAAKTLGFTPWQTMRLIIIPQTVSRVIPPVCNETITLVKDTAL
                     VTAIGVGELLKAAQGAVNRDVNITAYVVAALIYLAITFVMTMAYERLEKYFSRHQKKE
                     A"
     gene            complement(1319..1969)
                     /locus_tag="ACZPFK_09865"
     CDS             complement(1319..1969)
                     /locus_tag="ACZPFK_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1406662.1"
                     /translation="MGFRDENNELVGFDIDLATAVCEELGVKAVFQPIDWKAKEMELS
                     TGKIDCVWNGMSITPERQETMSLSNPYLNNKIIVMTNQGVEIADKEDLANYKLGTQAD
                     SSALEVIKADPVYASIQGQLTEYDTYDQVIMDMQAGRLDAMIVDEVLGQYKNTQMDNA
                     FGVASFDFGDDLYAIGFRKGDAELTKAVNDAIQACIDSGKAAEVSEKWFGADIMVQ"
     gene            2557..2632
                     /locus_tag="ACZPFK_09870"
     tRNA            2557..2632
                     /locus_tag="ACZPFK_09870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2590..2592,aa:Gly,seq:gcc)
BASE COUNT          519 a          820 c          745 g          577 t
ORIGIN      
        1 gaagagcatg atctgcggat cgatggccag cgcccgggca atggcgaccc gctgctgctg
       61 cccgccggag agcatggcgg gatagctgtc ggctttgttc tccagcccca ccagccgcag
      121 caggcggtcg gcgttggcga tggcgtcggc ccggttctgc tttaagatct ggatgggcgc
      181 aatggtgata ttttcaaggc aggtcaggtg gggaaacagg ttgaactgct gaaagaccat
      241 gcccgttttc atgcagatgc ggcgcagctc cttttcgggc gggtacttca cggcgccgtc
      301 caccgtctcc accagcgtat ccccgtccag gacgatgcgg ccgccggaga cccgctccaa
      361 attgttgaca cagcgcagca gggtggactt gccgctgccg gaggagccga tgatcgccac
      421 cgcctccccg cgctccaccg tgagcgagac gcccttgagg gcctcaaagc cgccaaagcg
      481 cttggtgacg ttttccactg tcagaatcgg cacggctcag gcctcctttt tttgatgacg
      541 gctgaaatat ttttccagcc gttcatatgc catggtcatg acaaaggtga tggcaagata
      601 gatcagcgcg gccaccacat aggcggtgat gttcacgtcc cggttcaccg caccctgggc
      661 cgctttcagc agctcgccca cgccgatggc cgtcaccagg gcggtgtctt tcaccagggt
      721 gatggtctcg ttgcagaccg gcgggatcac ccggctgacc gtctgcggga tgatgatcag
      781 gcgcatggtc tgccagggcg taaagcccag cgtctttgcc gcctcgtgct gcccccggtc
      841 gatggactgg attccgccgc ggtagatctc ggcaaaatag gccgcataat tgagcacaaa
      901 ggtaagcacc gcagccggga agcgatcaaa ggtcagcccc aggaacggca gcccgtaata
      961 gacgaaaaag agctgcagca tcagcggcgt gccccggaag atccagatat aggcccgcgc
     1021 cagccagcgc agcggcaata cccgggaaat gctgcccagc gtgaacacca gccccaaagg
     1081 cagtgaaaag atcagggtca gcgcaaaaat cttcaaggtc tgccaaagcc cctgcatcag
     1141 cgccggcatc aacagtgcaa tataggaaag gtccatccgg tctcctccac cctcgctgcc
     1201 gcctgcgcgg cttttttgcg gtcagacagc aaagccggcg ggtgcgcccc gcacccgccg
     1261 cttcgccggt cacgaactta tttattctat cacattgcgg cccggtgcgc cagccccgtc
     1321 actgcaccat gatgtcggcg ccgaaccact tttcagagac ctcggccgcc ttgccgctgt
     1381 cgatgcaggc ctggatggcg tcgttgaccg ccttggtcag ttccgcgtcc cccttgcgga
     1441 agccgatggc atacaggtcg tcgccgaaat caaaggacgc gacgccgaac gcgttgtcca
     1501 tctgggtgtt cttgtactgg cccagcacct cgtcgacgat catcgcgtcg agacggccgg
     1561 cctgcatatc catgataacc tggtcgtagg tgtcgtactc ggtcagctgc ccctggatgg
     1621 aggcatagac cggatccgcc ttgatgacct ccagcgccga ggaatccgcc tgggtgccca
     1681 gcttataatt ggccaggtcc tctttgtccg cgatctcgac gccctggttg gtcatgacga
     1741 tgatcttgtt gttcaggtac ggattcgaca ggctcatggt ctcctgccgc tccggcgtga
     1801 tggacatgcc gttccagacg cagtcgatct tgccggtgga gagctccatc tctttggctt
     1861 tccagtcgat gggctgaaag accgccttga cccctagctc ctcgcagaca gcggtggcaa
     1921 ggtcgatgtc aaaacccacc agctcgttgt tttcatcccg gaagcccatc ggcgcaaagg
     1981 tgtcgtcgag gccgatgacc agctccccct tggcctggat gtcggccagc gtggtggagt
     2041 acccgcagaa cccgcccctg ccgcgctgcc gccggaagcc gccgtgcccg cggagctgcc
     2101 cgcgtcggag ccgccgcagg ccgtaaaact caaagccagc gcagccgcca gcaaaacgga
     2161 tatcatcttt ttcatcttga accctctcct cattcttttc tctctcatgc ggccgccggc
     2221 cgccgctttc tcttgagcac ggccttatta tacttcattc tgataaagct gtaaagtgct
     2281 aattggataa aacatcaatc tttgtgcaga cggccttttt gggtgcagct gagcagttga
     2341 tattttatga cattctcaca aaaacacttt ttctccgtgt gctttttctt tttggatttt
     2401 ttcggtttct tcgcccttgc ggctgctctt ttccctcccc tcaaaaaagt aaaatttatc
     2461 cttgacaaac cccttgcttc tcccttataa taaataaggc ccactgatcc aggtgttgca
     2521 gcggttataa aaacgtctgt tttgggcata atgaatgcgg ctttagctca tctggtagag
     2581 cgccaccttg ccaaggtgga ggtagcgagt tcgagcctcg taagccgctc caatatggtg
     2641 ctgtagccaa gtggtaaggc a
//
LOCUS       DBMDYA010000411         2661 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8405_length_2661_cov_1.319141, whole genome shotgun sequence.
ACCESSION   DBMDYA010000411 DBMDYA010000000
VERSION     DBMDYA010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_2661_cov_1.319141"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="ACZPFK_09875"
     CDS             <1..573
                     /locus_tag="ACZPFK_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406663.1"
                     /translation="LRITPTARLLRRCPVYVSAGGFRGDDLPILALGADNAPVLQRLL
                     PEFCPPEGPLCNPRRKSPPQYLWKPGSLSLLLAALLAVSLRAVPAASPLLGLLLCLAL
                     ASTLCNLEGIFKEGFCKNANRTFTLSYCRGLTRHLVSLYTNALSLTITRMPQAASAGR
                     CDLRVHTPSRRTYHLRGVEFYRARALKFNL"
     gene            623..1456
                     /locus_tag="ACZPFK_09880"
     CDS             623..1456
                     /locus_tag="ACZPFK_09880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1406664.1"
                     /translation="MIKAVVFDLDGTLLNTIQDLANAANHALRTLGLPEHTTEEFLHF
                     VGDGRRNMVLRMLPEALRADEAMVARASGLFDEHYNAHLRDCTAPYPGIPELLAALKA
                     KGLRLGVVSNKPHEFVTQIIESYFPGLFDSVAGQQGGCIKPDPSGVKQVIDAFALHPD
                     QVLYVGDSAVDIETAQNAGTASCGVLWGFRDEAELEEAGAQYIAAHPQDIATLIASRN
                     RAEGFNRGLALFTVAVMAVCLVGVFYFLFTGNGAGIGACVVLPLLMGFAATSMLKKKK
                     E"
     gene            1461..1640
                     /locus_tag="ACZPFK_09885"
     CDS             1461..1640
                     /locus_tag="ACZPFK_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HIR1406665.1"
                     /translation="MADKRFKKIYTQGVMGTQEIWQDVETGVQYLYITAGYSGGLTPL
                     LGTDGRPVVTPVEPD"
     gene            complement(2009..>2661)
                     /locus_tag="ACZPFK_09890"
     CDS             complement(2009..>2661)
                     /locus_tag="ACZPFK_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1406666.1"
                     /translation="KWPDGGYLPSGGALAKRYGVSLYTVRQALQRMEALGFIQVQNGR
                     GAQVVLSRVGAHDRAYFAGPTQKRDALIYLQALHLTAVLLGPAAEAAFDRIGPDGLRR
                     IEEGLRCNGVAAQKACLDEVVNALPWEALQQIFHELGRLLLWGQHLLFRPRAASRMPE
                     LEHRCTEALERFRCGDRQGFVEAVAGSYDDSFRWVRSFLCASGLWEAGELRGRGEA"
BASE COUNT          419 a          980 c          822 g          440 t
ORIGIN      
        1 ctgcgcatca cccccaccgc ccggctgctg cggcgctgcc cggtctatgt cagcgccggg
       61 gggttccgcg gcgacgacct ccccatcctg gcactggggg ccgataacgc cccggtcttg
      121 cagcgcctgc tgccggagtt ctgcccgccg gaggggccgc tgtgcaaccc gcgccgcaaa
      181 agcccgcccc aatatctgtg gaagcccggc tccctctcgc tgctgctggc ggcgctgctg
      241 gccgtctcct tgcgggcggt gccggcggcg tcccccctgc tggggctgct gctctgcctg
      301 gcgctggcca gcaccctttg caacctggag ggcattttta aagagggctt ttgcaaaaac
      361 gctaaccgca cctttacgct cagctactgc cgcggcctga cccgccacct ggtctcgctg
      421 tacaccaacg ccctctcgct gaccatcacc cgcatgccgc aggccgcctc cgcggggcgc
      481 tgcgacctgc gcgtccacac gccctcccgc cgcacctacc atctgcgcgg cgtcgagttc
      541 taccgcgccc gggcgctgaa gttcaatctc tgacccggcg gccccgccgc tgcggatccc
      601 acatagaaag aagtcgatcc ccatgatcaa agctgttgtt ttcgacctgg acggaaccct
      661 gctcaacacc atccaggacc tggccaacgc cgccaaccac gccctgcgca ccctgggcct
      721 gcccgaacat accaccgagg agtttttgca ctttgtgggg gacggccgcc gcaatatggt
      781 gctgcggatg ctgcccgagg ccctgcgcgc cgacgaggcg atggtggcgc gggccagcgg
      841 cctttttgac gagcattaca acgcccacct gcgcgactgc accgcgccct atcccggcat
      901 ccccgagctg cttgccgccc tcaaggccaa gggcctgcgg ctgggggtgg tctccaacaa
      961 gccccacgag tttgtgaccc agatcatcga gagctatttc cccggcctgt tcgacagcgt
     1021 ggccggccag cagggcggct gtatcaagcc cgacccctcc ggggtcaagc aggtgatcga
     1081 cgcgttcgcc ctgcatcccg atcaggtgct ctatgtgggg gacagcgccg tggatatcga
     1141 gacggcgcag aacgcaggca ccgccagctg cggggtgctg tggggctttc gggacgaggc
     1201 ggagctggag gaggcgggcg cgcagtacat cgccgcccac ccgcaggaca tcgccaccct
     1261 catcgcctcc cgcaaccggg ccgagggctt caaccgcggc ctggcccttt ttaccgtggc
     1321 ggtgatggcc gtctgtctgg tgggcgtatt ctactttttg ttcaccggca acggggcggg
     1381 catcggcgcc tgcgtggtgc tgccgcttct gatggggttt gcggcgacga gtatgctgaa
     1441 aaagaaaaag gagtgatcgc atggcggaca agcgctttaa aaagatctat acccaagggg
     1501 tgatgggcac ccaggagatc tggcaggatg tggagacggg ggtgcagtac ctgtacatca
     1561 cggcgggcta cagcggcggg ctgaccccgc tgctggggac ggacggcagg cccgtcgtca
     1621 ccccggtaga gccggactga cggcaggcgt cgccggcacg gcaaaagcgc cgcatcccca
     1681 aggcgttccg cccgggagtg cagcccaatt ttctgcgcgc cgcaagcccg cgggcttgca
     1741 ggcggcggcg gtgcagcggg cgcgcctttt ccgccgtcat acggcgatat aggcccccat
     1801 caaaaccgca gtaagacccg gcagcctctt taaaggcgcc gggctttttt tgtgcaaaac
     1861 gccgctgcag cggcttggaa ccggggcgca gcgtatggga gccgccggcc ccaaaccgtc
     1921 gccctcccgc ttatcgtcgc cccccttttt tgctctgcaa agcgtttgcg cccgccgcgc
     1981 ggcttccgcc cccaccgcgg tccccgggtc acgcctctcc tctgccccgc agctcgccgg
     2041 cctcccacag ccccgaggcg caaagaaagg atcgcaccca gcgaaacgag tcgtcgtagc
     2101 tgcccgccac ggcttccaca aacccctgcc ggtccccgca ccggaaccgc tccagtgcct
     2161 ccgtgcatct gtgctccagc tccggcatcc gtgaggccgc ccgcggccgg aacagaagat
     2221 gctgccccca gagcagcagc cggcccaatt cgtgaaagat ttgctgcagg gcctcccagg
     2281 gcagcgcatt caccacctcg tcgagacagg ctttctgtgc cgccaccccg ttgcagcgca
     2341 gcccctcctc tatccgccgc aggccgtccg gccctatgcg gtcaaaggcg gcctctgccg
     2401 ccgggcccag cagcacggcg gtgaggtgca gggcctggag gtagatcagc gcatcccgct
     2461 tttgtgtcgg gccggcgaag tacgcccggt cgtgcgcccc cacccgggac aaaacaacct
     2521 gcgctcccct gccgttctgc acctggataa agcccagggc ctccatcctc tgcagcgcct
     2581 gccgcaccgt gtacagcgat accccgtacc gttttgccag cgccccgccc gagggcaggt
     2641 acccgccgtc cggccatttc c
//
LOCUS       DBMDYA010000412         2661 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8406_length_2661_cov_1.202344, whole genome shotgun sequence.
ACCESSION   DBMDYA010000412 DBMDYA010000000
VERSION     DBMDYA010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8406_length_2661_cov_1.202344"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="ACZPFK_09895"
     CDS             <1..976
                     /locus_tag="ACZPFK_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1406667.1"
                     /translation="AASLATLPVLVAVGGGVSVFSVLTNLITVPLLAPLLALGLFTAV
                     TAGAPVLAVFSRTAALLCGLLIRLLNAVTGWVGALPFGVVYLEGLYPLAVILCGFVLG
                     AVLFRLPAGKRRRGFAALAALFLLAGALMGRMLSAGTVRVALVGATQQPAVVITRDGQ
                     AAVLWRGGRTNTAAVENYLERQNVRQVALLVDGSGDASADSLLRRCAPLCSCDVKREV
                     AAGVTFCPFDDIILTVRRQGEGDLYLIDASGVRLLCATGKTDLEGWPGVDVFLAGTQL
                     PSSLRAQTVLLGGREAGWVDGCGLPLLRGEEPAVWLRPGGSIKFLGVG"
     gene            977..2053
                     /gene="holA"
                     /locus_tag="ACZPFK_09900"
     CDS             977..2053
                     /gene="holA"
                     /locus_tag="ACZPFK_09900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1406668.1"
                     /translation="MAVLDLAGLNRVIDTEKKVPVYFLYSPDEYLLDSFAQRLLRMLS
                     ATEGEEPTVIPGPQPQMGAVVAAAGAISLFGTKRLVYLKRIEPTAMKADDIKLLCELM
                     EECESAVLLLTMMVKEPEKSWRGPAKLTVPAAAKPLLAAAEKAGLAAGLDKPGEASAA
                     RFAARKAQELGAAFAPGAAEELVGRCGIDLFLLSGEVEKLAAASGYGEITRDTVRALS
                     ARNIETDVFEMVRALMAGRSRDAFAKLGELLYLQNDPIAIAAAINGSFVDMARVKAAS
                     IQGVGYQQAFKAMGNKGSDYRYKKSLETARPFSMQQLTAFLEVLDRLDRRLKGSAVDK
                     GTLLQMALGEILLVGRGELNYAHS"
     gene            2040..>2661
                     /locus_tag="ACZPFK_09905"
     CDS             2040..>2661
                     /locus_tag="ACZPFK_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1406669.1"
                     /translation="MRIRELLIVEGKYDAAKLSGLVDGLILTTGGFSIYRDPEKRALI
                     RDLGRKRGIIILTDSDAAGFQLRNYIQNFARGAKIKNAYIPAVEGKEKRKKAPSKEGT
                     LGVEGLPAEVLLTALRRAGATEEAPRAGRRLTYTDLYRLGISGTAGSAARRRELLASI
                     GLPLRLSKKALLETLDAGYTYEELAAICEKKPVLFWDFHGTLTQPES"
BASE COUNT          422 a          803 c          959 g          477 t
ORIGIN      
        1 cgccgcctct ctggccacgc tgccggtgct ggtggcggtg ggcgggggtg tttccgtctt
       61 ttcggttctc accaatctga ttaccgtgcc gctgttggcc cctctcctgg cgctggggct
      121 cttcaccgca gtgacggcgg gcgcaccggt gctggcggtg ttcagccgca ctgcggcgct
      181 gctctgcggc cttttgattc gtctgctcaa cgccgttacc gggtgggtgg gcgcgctgcc
      241 ctttggggtc gtctatctgg aggggctgta tccgctggcg gtgatcctgt gcgggtttgt
      301 gctgggggcg gtgctgttcc gcctgcctgc gggcaaaaga cggcgcgggt ttgccgcgct
      361 tgcggctctt tttctgttgg cgggcgccct gatggggcgg atgctgtccg cgggcacggt
      421 gcgggtggcg ctggtgggcg ccacccagca gccggctgtg gtgatcaccc gggacggaca
      481 ggccgcggtg ctctggcggg gcggccggac caacacggcg gcggtggaaa attacctcga
      541 gcgccagaac gtgcgccagg tggcgctgct ggtggacggc agcggagacg cctccgcaga
      601 cagcctgctg cggcgctgcg cccctctgtg cagctgtgac gtgaagcgcg aggtggcggc
      661 gggcgttact ttttgcccgt ttgatgatat aatattgacg gtgcgccgcc agggagaggg
      721 cgacctgtac ctgatcgacg cgtccggcgt ccggctgttg tgcgccacgg gcaagaccga
      781 cctggagggc tggcccggcg tcgacgtttt tctggccggc acccagctgc cgtcgtccct
      841 gcgggcgcag acggtgctgc tgggcggcag ggaggcgggc tgggtggacg gctgcgggct
      901 gcccttgctg cgcggggagg agccggcggt ctggctcaga ccgggcggtt cgataaaatt
      961 tttgggggtg ggatagatgg cggtcttgga tctggccggg ctcaaccggg tgatcgacac
     1021 tgaaaaaaag gtgccggtgt atttcctcta ttcccccgat gaatatctgc tcgactcgtt
     1081 tgcccagcgg ctgctgcgga tgctctccgc caccgagggg gaggagccca ccgtgatccc
     1141 cggcccgcag ccccagatgg gcgcggtggt tgccgccgcc ggagccatct cgctgttcgg
     1201 taccaaaagg ctggtctatt tgaagcggat cgaacccacg gccatgaaag cggacgacat
     1261 caagctgctt tgcgagctga tggaggagtg cgagagcgcg gtgctgctgc tgaccatgat
     1321 ggtcaaagag cccgagaaga gctggcgcgg cccggcaaag ctcacggtgc ccgcggcggc
     1381 aaagccgctg cttgccgccg ccgaaaaagc aggccttgcc gccgggctgg acaagccggg
     1441 cgaggcctcg gcggcccggt tcgccgcccg caaggcgcag gagctggggg cagcatttgc
     1501 ccccggcgcg gctgaagagc tggtgggccg ctgcggcatc gacctttttc tgttgagcgg
     1561 cgaggtggaa aagctggccg ccgccagcgg ctacggtgag atcacgcggg acacggtgcg
     1621 ggcgttgtcg gcccgcaaca tcgagacgga cgtctttgag atggtgcgcg ccctgatggc
     1681 ggggcgcagc cgcgacgcgt tcgcaaagct gggcgagctg ctctatctgc aaaacgaccc
     1741 catcgccatc gcagcggcca tcaacggcag ctttgtggat atggcgcggg tcaaggccgc
     1801 gtcgatccag ggggtcggct accaacaggc tttcaaggcc atgggcaaca agggcagcga
     1861 ttaccgctac aaaaaaagcc tcgagaccgc gcgccccttt tccatgcagc agctcaccgc
     1921 ctttctcgag gtgctggatc ggctggaccg ccgcctcaag ggcagcgccg tggacaaagg
     1981 caccctgctg cagatggcgc tgggcgagat tttactggtg ggcagggggg agctgaatta
     2041 tgcgcattcg tgaactgctg atcgtggagg gcaagtacga cgcggcaaag ctgtcgggct
     2101 tggtggacgg cctcatcctc accaccggcg gcttttccat ctaccgcgat ccggaaaagc
     2161 gggccctgat ccgcgacctg ggcagaaagc gcggcatcat cattttgacc gattcagacg
     2221 cggccggttt tcagctgcgc aattacatcc agaatttcgc ccgcggagca aagatcaaaa
     2281 acgcctacat ccccgccgtg gagggcaagg agaaacgcaa aaaagccccc tcaaaagagg
     2341 ggacgctggg ggtggaggga ctgcccgcgg aggtgttgtt gacggccctg cgccgggccg
     2401 gcgccaccga ggaggccccg cgcgcagggc ggcggctcac ctataccgat ctttaccggc
     2461 tgggcatctc gggcacggcg ggcagcgccg cccgccgccg agagctgctg gcctccatcg
     2521 gcctgccgct gcggctgtcg aagaaagcgc tgctggagac gctggacgcc ggttatactt
     2581 atgaagagct ggcggcgatc tgtgaaaaaa aaccggtgct gttctgggat tttcacggta
     2641 ccctcaccca gccggaatcc g
//
LOCUS       DBMDYA010000413         2659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8411_length_2659_cov_1.299062, whole genome shotgun sequence.
ACCESSION   DBMDYA010000413 DBMDYA010000000
VERSION     DBMDYA010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8411_length_2659_cov_1.299062"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="ACZPFK_09910"
     CDS             <1..200
                     /locus_tag="ACZPFK_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406670.1"
                     /translation="TKSYKKAAVSTNLTPRPFCTVLFAAVSLIKGDIESLSPPRRPSG
                     AAGTKAKGDMYVEQSIPEICL"
     gene            166..1518
                     /locus_tag="ACZPFK_09915"
     CDS             166..1518
                     /locus_tag="ACZPFK_09915"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1406671.1"
                     /translation="MSNNPFQKFAFERILAYLQKDPEKHLPQLMEAVDRFAGEGPDSF
                     PTQRAVFRGIVNDPSSNWYQLILRVIRDTDPAVLNKIFYNFFLNANLIGWKKQEEVRR
                     NHHCNAPWAILLDPTSACNLHCTGCWAAEYGDRLNLSLEEIDGIITQGKELGVYLYIY
                     TGGEPLVRKADLIRLCEKHDDCVFLSFTNATLIDEAFADEMLRVKNFIPAISVEGFEA
                     ATDGRRGEGTFRKVTRAMELLQKKKLPFGISCCYTSQNFDDITSEAYYDQMIRWGAAF
                     VWYFHYMPVGADAVTALLPTPQQREYIYHKIREYRRTKAIFGMDFQNDGEFVGGCIAG
                     GRRYLHINANGDADPCVFIHFSDSNIREKTLLEVLTSPLFMAYHDGQPFNSNHLRPCP
                     MLENPEKLQELVARTGAHSTDLQCPESTAQLCGRCKAYADRWQPVAEQLWAASGHGGK
                     "
     gene            complement(1710..2396)
                     /locus_tag="ACZPFK_09920"
     CDS             complement(1710..2396)
                     /locus_tag="ACZPFK_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR1406672.1"
                     /translation="MSKVSVYVENMFAGLPHTRQAAAQKAEAKQRLEARYAALLAEGK
                     NEHEALGIVVAENDDLDDLRAKLGIRRGDAAKETLLAEYEAFTRRFAGGISAGVVLCL
                     LAVAALIVLSGLMEPGSSLPGALFFVLAAAGVGIFVFYGIKNGHYLERLQAAGCEPEP
                     LTLQRRKRLCDRISSAIMAAAAVVFVSMGVAGLWHPGWVALPIGGILCAVVWLILDGG
                     RGDSGEPEKK"
BASE COUNT          491 a          937 c          774 g          457 t
ORIGIN      
        1 cgacaaaatc atacaaaaag gccgcggtgt caacaaatct gacaccgcgg cctttttgta
       61 cagtgctttt cgccgccgta tcccttataa agggggatat agaatctctg tccccgccgc
      121 gccgccccag cggcgcggcg gggacaaaag cgaaaggtga tatgtatgtc gaacaatcca
      181 ttccagaaat ttgcctttga gcgcatcctg gcttatctgc aaaaagaccc cgaaaagcac
      241 ctgccgcagc tgatggaggc ggtggaccgg ttcgcagggg agggaccgga cagctttccc
      301 acccagcggg ccgtgttccg cggcatcgtc aacgatccct cctccaactg gtaccagctc
      361 atcctgcgcg tcatccggga caccgacccg gcggtgctga acaagatttt ttacaacttt
      421 tttctcaatg ccaacctgat cggctggaaa aagcaggagg aggtgcgccg gaaccatcac
      481 tgcaacgcgc cctgggccat tttgctggac cccaccagcg cctgcaacct gcactgcacc
      541 ggctgctggg ccgccgaata cggcgaccgg ctcaacctga gccttgagga gatcgacggc
      601 atcatcaccc agggcaagga gctgggcgtc tatctgtaca tctacaccgg cggcgagccg
      661 ctggtgcgca aggccgacct gatccggctg tgcgaaaagc acgacgactg cgtctttctc
      721 tctttcacca acgccacgct catcgacgag gcctttgccg acgagatgct gcgggtgaaa
      781 aacttcatcc ccgccatcag tgtggagggc tttgaggctg ccaccgacgg gcggcggggc
      841 gagggcactt tccgcaaggt cacccgcgcc atggaactgc tgcaaaagaa aaagctgccg
      901 ttcggcatca gctgctgcta caccagccag aacttcgacg acatcacctc ggaggcctac
      961 tacgaccaga tgatccggtg gggcgcggcc tttgtctggt acttccacta catgccggtg
     1021 ggcgcggacg cggtgaccgc cctgctgccc accccgcagc agcgggaata catctatcac
     1081 aaaatccgcg aatatcggcg caccaaggcc atcttcggca tggattttca aaacgacggc
     1141 gagtttgtgg gcggctgcat cgcgggcggc cggcgctacc tgcacatcaa cgccaacggg
     1201 gacgccgacc cctgcgtctt catccacttc tccgattcca acatccggga aaaaacgctg
     1261 ctggaggtgc tgacctcgcc gctgtttatg gcttatcacg acggccaacc ctttaacagc
     1321 aaccacctgc gcccctgccc catgctggaa aacccggaaa agctgcagga gctggtggcc
     1381 cgcaccggcg cgcactccac cgacctgcaa tgcccggaga gcaccgccca gctctgcggc
     1441 cgctgcaagg cctacgccga ccgctggcag ccggtggccg agcagctctg ggccgcgagc
     1501 gggcacggcg gcaaatagca gccccctccc cggcgggcgg ttcaaaagcg gcgcgcccct
     1561 ttgggcccgc gcggcgcgag aggcgcctca cacaaaacga cagcccggcg ctgagagccg
     1621 agagaaacgg gacctttgtg gtccccctct tcctccgctc aaaacgccgg gctgcttttt
     1681 ttgtttttcc cgcgggctga cgcctgcggt catttttttt cgggctcccc gctgtcgccg
     1741 cgtccgccgt ccaggatcag ccagaccacc gcgcagagga tgccgccgat gggcagcgcc
     1801 acccagcccg ggtgccagag ccccgccacc cccatcgaga cgaagaccac cgccgccgcc
     1861 gccatgatgg cgctgctgat gcggtcgcac agccgcttgc gccgctgcag ggtgagcggc
     1921 tccggctcgc agccggcagc ttgcaagcgc tcgaggtaat ggccgttttt gatgccgtaa
     1981 aagacaaaga tgcccacgcc cgcggccgcc agcacaaaga acagcgcgcc cggcaggctg
     2041 gaccccggct ccatcaggcc cgacagcacg atcagcgccg ccaccgccag caggcagagg
     2101 acaacgcccg ccgagatgcc gcccgcaaag cgccgggtaa aggcctcata ttccgccagc
     2161 agcgtctctt tggccgcgtc cccgcgccgg atgcccagct ttgcccgcag gtcgtccagg
     2221 tcatcgtttt cagccaccac gatgcccagc gcctcgtgct cgtttttccc ctcggccagc
     2281 agcgccgcat agcgcgcctc cagccgctgt tttgcctccg ctttctgggc cgccgcctgg
     2341 cgggtgtggg gcagcccggc aaacatattt tccacataaa cgctgacttt gctcattttg
     2401 ctctccctta aaacccgacg gcgttttccg cgcgccggcc cccctgcagg gctttcaacg
     2461 gcggcggcag gggccgggcc ggcggtgcgc gccgcgcggg ggcggccatg ttctgcgccc
     2521 cgccgccggc gctgcgcgcc gtcttttccc ctggcgccga tccacctctt gagtatagca
     2581 cagggccgct gctttcgtaa agttgctttt tggaaaaaac gggatttccg cctcccggcg
     2641 gtcctcgccg cccccctgc
//
LOCUS       DBMDYA010000414         2657 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8418_length_2657_cov_1.444053, whole genome shotgun sequence.
ACCESSION   DBMDYA010000414 DBMDYA010000000
VERSION     DBMDYA010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_2657_cov_1.444053"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="ACZPFK_09925"
     CDS             complement(<1..291)
                     /locus_tag="ACZPFK_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1406673.1"
                     /translation="MVDYRIRTFLALYEQMNYRRTAELLRMSQPAVTQQIHALEREYG
                     CRLFVYDGRRLHRTPQADQVAEFARVAVYNEQRMLRELGRPQVRAVRLGATKT"
     gene            437..>2657
                     /locus_tag="ACZPFK_09930"
     CDS             437..>2657
                     /locus_tag="ACZPFK_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1406674.1"
                     /translation="MKVLVIGGVAAGTKAAAKIKRQDRGAEVKILTKGADISYAGCGL
                     PYYVGGMIPSREALIVNTPEKYEGLTGAQVVTGCEVTGVDFAAKAVTAVRDGHTFSEG
                     YDKLIIATGAVPFVPQLPGVDLPGVFCMRAPDDAIGLRGYVQQNRCRSAVVVGAGFIG
                     LEIAENLHAQGLSVTVIDAAPQIMPNAFDPEMADYARRKLRGAGIRVLTGRSLSAVTG
                     EGRAQGVATGSGDLAADVVVLAIGVRPATAFLEGSGLEMFKGTVLVDEYMRTNVADVY
                     AAGDCAMVHNSLTGQPQWSAMGSTANLSARALACTLTGTPAVYGGCLGTGVVRLLDGL
                     NAGRTGLTEAQALAAGYDAAAVVCVTDDKAHYYPGAGAFITKLVADRATHRVLGVQVL
                     GAGAVDKMVDVAVTGISQGMRLEQFDTLDFAYAPPFSTAIHPFVQACYVLENKLSGAL
                     VSMTPAEYAAGAAKGYRVLDVQPAPSIPGAQWIDLSKVDGELPGIARDEKLLLVCARG
                     KRGYFLQNRLRRAGYTATKVLEGGVTFNEVKVEFEGDALPPAEIKRVKGLGCLQDKRY
                     PDRFNVRIITRNGKLTAEEQRAVAEAAERFGSGEVTMTTRLTLEIQGVPYAKLEEAMA
                     FVTEAGLETGGTGSKVRPVVSCKGTTCQYGLCDTFALSQKLHDLFYVGYHGVDLPHKF
                     KLAVGGCPNNCVKPNLNDLGIVGQRLPEADLSKCRGCKSCQVESACPIGAAKMVDGRL
                     "
BASE COUNT          447 a          874 c          926 g          410 t
ORIGIN      
        1 agtcttggtc gcccccagcc gcaccgcgcg cacctgcggg cgtcccagct cgcgcagcat
       61 ccgctgctcg ttgtacaccg ccacccgggc gaactccgcc acctggtcag cctgcggggt
      121 gcggtgcagc cggcgcccgt cgtagacgaa caggcggcag ccgtactccc gctccagcgc
      181 gtggatctgc tgggtcaccg ccggctggct catgcgcagc agctcggccg tgcggcggta
      241 gttcatctgc tcgtacaggg ccagaaaggt gcggatgcga tagtcgacca cgggatcacc
      301 tcataagaaa aattaatcaa cagataagat ttaaaaactt tattttatta taggataagt
      361 ggtatacttt gacaagacgt gcgctcatcc gacccggggc ggtggatggg cgtcaaaaaa
      421 cgggaggcag ggttttatga aagtactggt catcggcggc gtagccgccg gcacaaaagc
      481 ggccgccaag atcaagcggc aagaccgcgg cgccgaggtg aagatcctca ccaagggcgc
      541 ggacatctcc tacgcgggct gcggcctgcc ctattacgtg ggcggcatga tccccagccg
      601 cgaggcgttg atcgtcaaca cgcccgaaaa gtatgagggc ctgaccggcg cgcaggtggt
      661 gaccgggtgc gaggtgaccg gggtggattt tgccgccaag gccgtcaccg ccgtgcggga
      721 cgggcacacc ttcagcgagg gctatgacaa gctcatcatc gccaccggcg ccgtcccctt
      781 tgtgccgcag ctgcccggcg tggacctgcc cggcgttttt tgcatgcgcg cgccggacga
      841 cgccatcggg ctgcgcgggt atgtgcagca gaaccgctgc cgcagcgcgg tggtggtggg
      901 cgcgggcttc atcggcctgg agatcgccga aaatctgcac gcccagggcc tgtcggttac
      961 cgtgatcgac gcggcgccgc agatcatgcc caacgccttt gaccccgaga tggccgatta
     1021 cgcgcgccgc aagctgcgcg gggcgggcat ccgggtgctg accggccgca gcctgtccgc
     1081 cgtcaccggc gagggtcggg cccagggggt ggccaccggc agcggcgacc tggccgccga
     1141 cgtggtggtg ctcgccatcg gcgtgcggcc cgccaccgcc tttctcgagg gcagcgggct
     1201 ggagatgttc aagggcaccg tgctggtgga cgagtacatg cgcaccaatg tggccgacgt
     1261 ctacgccgcg ggcgactgcg ccatggtaca taacagcctg accggccagc cccagtggtc
     1321 ggccatgggc tccaccgcca acctctcggc ccgagcgctg gcctgcaccc tcaccggcac
     1381 ccctgcggtc tatggcggct gcctgggcac cggcgtggtg cggctgctgg acgggctgaa
     1441 cgccgggcgc accggcctga ccgaggcgca ggcgctggcc gccggctacg acgcggccgc
     1501 ggtggtctgc gtcaccgacg acaaggccca ctattacccc ggcgcggggg ctttcatcac
     1561 caagctggtg gccgaccggg ccacccaccg ggtgctgggc gtgcaggtgc tgggcgccgg
     1621 cgcggtggac aagatggtgg acgtggcggt gaccggcatt tcgcagggca tgcggctgga
     1681 gcagttcgac acgctggact ttgcctatgc gccgcccttt tccaccgcca tccacccctt
     1741 tgtgcaggcc tgctacgtgc tggaaaacaa gctgtccggc gcgctggtgt ccatgacccc
     1801 ggccgaatac gccgccggcg cggccaaggg ctaccgggtg ctggacgtgc agcccgcccc
     1861 cagtatcccc ggcgcccagt ggatcgacct gtcaaaggtg gacggcgagc tgcccggcat
     1921 cgcccgggat gaaaagctgc tgctggtctg cgcccgcggc aagcgcggtt actttttgca
     1981 gaaccggctg cgccgcgccg gctataccgc caccaaggta ctggagggcg gcgtgacttt
     2041 caacgaggta aaagtggagt ttgagggcga cgcgctgccc cccgccgaga tcaagcgggt
     2101 caaggggctg ggctgcctgc aggacaagcg ttacccggac cgcttcaacg tgcgcatcat
     2161 cacccgcaac ggcaagctga ccgcggagga gcagcgcgcg gtggccgagg ctgcggagcg
     2221 gttcggcagt ggcgaggtga ccatgaccac ccgcctgacg ctggagatcc agggcgtgcc
     2281 ctacgccaag ctggaagagg cgatggcctt tgtaaccgag gcgggcctgg agaccggcgg
     2341 caccggctcg aaggtgcggc cggtggtgtc ctgcaagggc acgacctgcc agtacggcct
     2401 gtgcgacacc tttgcgctga gccaaaagct gcacgacctg ttctatgtgg gctaccacgg
     2461 cgtggacctg ccccacaagt tcaagctggc ggtgggcggc tgccccaaca actgcgtcaa
     2521 gcccaacctg aacgacctgg gcatcgtggg ccagcgcctg cccgaggcgg acctgtcgaa
     2581 gtgccgcggc tgcaagagct gccaggtgga gagcgcctgc cccatcggcg cggccaaaat
     2641 ggtggacggc aggctgg
//
LOCUS       DBMDYA010000415         2653 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8430_length_2653_cov_1.449843, whole genome shotgun sequence.
ACCESSION   DBMDYA010000415 DBMDYA010000000
VERSION     DBMDYA010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8430_length_2653_cov_1.449843"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="ACZPFK_09935"
     CDS             complement(<1..608)
                     /locus_tag="ACZPFK_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1406675.1"
                     /translation="MPKKQPVYNNESISSLKGAERVRKRPGVIFGSDGIEGCEHAVFE
                     ILSNAIDEAREGYGDRVSVTLFKDGSIEVEDFGRGIPVDYNKKEERYNWELLFCEMYA
                     GGKYDAAASNYEYSLGLNGLGLCATQYASRWMKADVYRDGAHYHLDFERGENVGGLQK
                     EPTKSKRTGTVIRWLPDREVFTDIDIPVDYFKDVMKRQAVVNA"
     gene            complement(960..>1595)
                     /locus_tag="ACZPFK_09940"
     CDS             complement(960..>1595)
                     /locus_tag="ACZPFK_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1406676.1"
                     /translation="LQSVDGIYGAEDDTDYARVATLAMIAARVRDATGPDFKIGVQLM
                     FNCITPSLAVAKAVNADFIRCSVLVGASDSTYGPVVSNTLKVMNYRRQIEAQNVGMIA
                     EISGYHNKSGTEEGLVGALASTAMRAGANAVEVCSRDEALNEQLVLSAKKAGEIPVIL
                     GGSTTVENCQRRLRYADGALVGSEFEGGRWGGPVIESIVEQYCKKVRQIEG"
     assembly_gap    1596..1605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1606..1780)
                     /locus_tag="ACZPFK_09945"
     CDS             complement(<1606..1780)
                     /locus_tag="ACZPFK_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015402.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1406677.1"
                     /translation="MEKTIFDDLGGKKLVLGLIHLKPMPGTPFYEDGNLEASLKKALG
                     DAKALQNGGADGCL"
     gene            complement(1794..>2653)
                     /locus_tag="ACZPFK_09950"
     CDS             complement(1794..>2653)
                     /locus_tag="ACZPFK_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406678.1"
                     /translation="DSVVMATPTTGTLAAKAGCTTLGITAHPESRMGVQAQYILQLEI
                     PQLPVSSIASQTRNYLAALMTTLLMGLHAGRVRGVISERQVEERRAEILRYVRAVCDP
                     GVLDRVDDQMLRLASAGWKDYLGYDFVGAGSDFATAYFGTAKFFEYYGALSCLNDSED
                     WCHINYFMRDRGRIGSIVVASQNSASFSRSVETIASMQKSGRNVLVVTDAAPEAFIEG
                     VTVCRLPGTDYAYISPLMNYIPLCMLGNYIALLNNYEYFGGMGPENPLFSQEGGINTI
                     KSSKIVLID"
BASE COUNT          492 a          850 c          699 g          602 t
ORIGIN      
        1 gcgttgacca cagcctgccg cttcatcacg tctttgaaat agtccaccgg gatgtcgatg
       61 tcggtgaaga cctcccggtc cggcagccag cggatgacgg tgccggtgcg cttggacttt
      121 gtgggctctt tctgcaggcc gccgacattc tcgccccgct caaaatccag atgatagtgc
      181 gccccgtcgc ggtagacgtc cgctttcatc cagcgggagg cgtattgggt ggcgcagagg
      241 cccagaccgt tcaggcccag ggagtactcg tagttgctgg ccgcggcgtc gtatttgccg
      301 cccgcataca tctcgcagaa gagcagctcc cagttgtagc gctcctcctt tttgttgtag
      361 tccacgggga tgccgcggcc aaaatcctcc acctcgatgg agccgtcttt gaacagggtg
      421 accgacacgc ggtcgccata gccctcgcgg gcctcgtcga tggcgttgga caagatctca
      481 aacaccgcgt gctcgcagcc ctcgatgccg tcggagccga agatgacgcc cgggcgcttg
      541 cgcacccgct cggccccctt gaggctggag atgctctcgt tgttgtatac cggttgcttt
      601 ttgggcatgc ttgcctcctg taaggttcgt ttcgaaaagc cgcacccggg gacagtgccc
      661 gggtgcgcgt tcgttctctc ttttgatgtt ccttttttat taaacacttt ttattgtgtt
      721 tttgtcaaga agaacgcgcc atatctggta ttccttattc ccactctccc ccctctttat
      781 cccagccccg ccgcactttt ctcccgcaca aaaatatccc cgggaaattc ccggggatat
      841 tttttactga aaagtattcc ggctgcgcac cggccggtca aaccgtctct ccgacgcgtc
      901 aaaaattttt tctcttgctt cttcagcttg ctcgggacgc atctttccga gcagtctttt
      961 cagccctcga tctgccgcac ttttttacag tactgctcca cgatgctctc gatgacgggc
     1021 ccgccccagc gcccgccctc aaactcgctc cccaccaggg cgccgtccgc atatctgagg
     1081 cggcgttggc agttttccac cgtcgtgctg ccgcccagga tcacagggat ttcccccgcc
     1141 tttttcgcac tgagcaccag ctgctcattg agggcctcat cccggctgca cacctccacg
     1201 gcattcgccc cggcgcgcat cgccgtgctg gccagcgccc ccaccaggcc ctcttccgtg
     1261 ccgctcttgt tgtgatagcc ggagatctct gcgatcatgc ccacattctg ggcctcgatc
     1321 tggcgtcgat aattcatcac cttaagggta ttgctgacca caggtccata ggtggaatcg
     1381 ctcgccccca ccagcacgga acaacggatg aaatccgcat ttaccgcctt ggccaccgcc
     1441 agagacgggg tgatgcagtt gaacatcagc tgtacgccga tcttaaaatc cggccccgtc
     1501 gcatcccgca cgcgggccgc gatcatcgcc aaagtcgcca cccgggcgta atcggtatcg
     1561 tcctcggcgc cgtaaatacc gtccacagac tgcagnnnnn nnnnngcagg caaccgtccg
     1621 cgcccccgtt ctgcagcgct ttggcatcgc ccagggcctt tttcagcgac gcctccagat
     1681 tgccgtcctc ataaaacggg gtgccgggca tgggtttgag gtggatcaag cccaatacca
     1741 gcttttttcc tcccaggtcg tcaaaaattg ttttttccat ggttttgccc ctttcagtcg
     1801 atcaagacga ttttgctgga tttgatggta ttgatcccgc cctcttggct gaacagcggg
     1861 ttttcaggcc ccatcccccc aaagtattca tagttgttga gcagagcgat ataattgccc
     1921 agcatgcaca gcgggatata gttcatcagc gggctgatat atgcataatc cgtgcccgga
     1981 agacggcaga ccgtcacgcc ctctataaag gcctccggcg cggcgtcggt gacgaccagc
     2041 acgttgcggc cggatttttg catggaggct atggtctcca ctgagcggct gaacgaggcg
     2101 gaattttggg acgccaccac gatactgccg atgcggccgc ggtcgcgcat aaaataattg
     2161 atatggcacc agtcttcgct gtcattgaga cagctgagcg cgccgtaata ttcaaaaaat
     2221 ttggccgtcc caaaataggc ggtggcgaaa tcactgcccg cacccacaaa atcatagccg
     2281 agataatctt tccatccggc gctcgccagc cgcagcattt gatcgtcgac gcgatccagc
     2341 acgccggggt cgcacaccgc ccggacataa cgcaggatct ccgccctgcg ctcctccacc
     2401 tgccgctctg agatgacgcc ccgcactctg cccgcgtgca gccccatcag taaagtcgtc
     2461 atgagggccg ccaaataatt gcgcgtctgt gaagcgatgg aggagacggg cagctggggg
     2521 atttccaatt gaagaatgta ttgtgcctgc acgcccatac gggattccgg atgggcggtg
     2581 atgcccaagg tcgtgcagcc cgcttttgcc gccaaagttc ccgtcgtcgg cgtggccatc
     2641 acgaccgaat cct
//
LOCUS       DBMDYA010000416         2650 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8442_length_2650_cov_1.195763, whole genome shotgun sequence.
ACCESSION   DBMDYA010000416 DBMDYA010000000
VERSION     DBMDYA010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_2650_cov_1.195763"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="ACZPFK_09955"
     CDS             complement(<1..688)
                     /locus_tag="ACZPFK_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HIR1406679.1"
                     /translation="MKKGRPSVTKKKITLTALLIAGVLSLFAFLFVQDVSRQLWRQSV
                     NTIRESTRQGLNTLQVQLRREFETIGAMALRLKAVPSTRREVLENDLANYSMLDEGTR
                     LYLEDGTCYPTAEERDEAAADRLFAESRKSGILDPHISSVTGVNVFNLFIRLNLADGT
                     AAYLAKEYEVDSIVDSFSLSFYQNAGFSYVVDSDGNVLIRPPHPNSNKTVQNLFDILE
                     ESPNDPSKLQL"
     gene            complement(685..1962)
                     /locus_tag="ACZPFK_09960"
     CDS             complement(685..1962)
                     /locus_tag="ACZPFK_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1406680.1"
                     /translation="MKSKGIKRTAICLAAALAFSGCGAKNVVSVEPEISGEITTITFF
                     GNKYEPENVRVIEEIISDFMVENPDIRVSYESLKGNDYYVALEKRLAAGKGDDVFMVN
                     HDTLLTLEAGGQVAELTQLALTAGYTEQMLDQMTDGGKIFWLPTTVSAFGLYCNLDLL
                     KAHGQQVPETLAEWKAVCDYFVAQGITPVVANNDISLKTLAIGAGFCEAYQDGTAPQA
                     FARLNRGEEQLSAFLGAGFSLVKEFIDKGYVDAEKTLNTKKTSGDLAEFVQGKAPFML
                     TGAWAAGRVKEMAPGFRFEVTALPVLEEGALLVLNADTRLGLNAASPNQEAALKFIEY
                     FIRPENIEKFADQQCSFSPLNEKSEPSLKELRPLLACYEDGPTVIGTDGRLELQIWEL
                     TAEATQKLLAGEPLQQVMQWMDQQAAAESEGAE"
BASE COUNT          504 a          844 c          685 g          617 t
ORIGIN      
        1 ataattgcag ttttgaggga tcgttggggg attcctccag aatatcaaac aggttctgta
       61 ctgtcttgtt gctgttgggg tggggcggcc ggatcagaac atttccgtcg gaatccacca
      121 cataggaaaa tcccgcgttc tgataaaatg agaggctgaa gctgtccaca atggaatcca
      181 cctcgtactc cttcgccagg tacgcggccg tcccgtccgc caggttcagc cgtataaaca
      241 gattgaacac attgaccccg gtcacgctgc tgatatgggg atccaggatg ccgctttttc
      301 ggctttcggc gaacaggcgg tccgccgccg cttcgtcgcg ctcctccgcc gtgggatagc
      361 aggtcccatc ctccagatag agcctagtcc cctcgtccag catgctgtaa ttggcaagat
      421 cattttccaa tacctcgcgc cgcgtagacg gcaccgcttt caagcgcagg gccatggcgc
      481 cgatggtctc aaactccctg cgcaactgta cctggagcgt gttcagcccc tgccgtgtac
      541 tctcgcggat ggtgttgacc gactgccgcc aaagctgccg gctcacatcc tgtacaaaca
      601 gaaaggcaaa cagggacaaa acgcccgcga tgaggagggc cgtcagggtg atttttttct
      661 ttgtgacgct ggggcgtccc tttttcattc cgctccctcg ctttccgcag cagcctgctg
      721 atccatccac tgcatcacct gctgcagcgg ctcgccggcc agcagcttct gcgtggcctc
      781 ggcagtcagc tcccagatct gcagctccag cctgccgtcc gtcccgatga cggtcgggcc
      841 atcctcatag caggccagca ggggccgcag ctctttcagg gacggctcgc tcttttcgtt
      901 caacggggaa aaggagcact gctgatccgc aaatttttct atattttccg gccggataaa
      961 atattcgata aacttcaagg cggcttcctg gtttgggctc gcggcgttca ggccgaggcg
     1021 ggtgtcggcg ttgagcacca gcaacgcacc ctcctccagc acgggcaagg ccgtcacctc
     1081 aaaccgaaag ccgggcgcca tctctttcac tcggccggcc gcccaggcac ccgtcagcat
     1141 aaaaggcgct ttgccctgca caaactccgc cagatcgccc gatgtctttt tggtgttcag
     1201 tgttttctct gcgtccacat atcccttgtc gataaactct tttaccagag aaaatccggc
     1261 gccgagaaaa gcgctcagct gttcttcccc cctgttaagg cgggcaaagg cctgtggggc
     1321 ggtgccgtcc tgataggcct cgcaaaaccc tgcgccgatg gcgagggtct tgagggaaat
     1381 gtcattgttc gccaccacgg gcgtgatacc ctgtgcgacg aaatagtcgc acaccgcttt
     1441 ccattccgcc aatgtctccg gcacctgctg tccgtgcgcc ttgagcagat ccagattgca
     1501 atacagccca aacgcagaaa cagtcgtcgg cagccaaaaa atcttgccgc cgtcggtcat
     1561 ctggtccagc atctgctccg tatagcccgc cgtcagggcg agctgcgtca gctccgcgac
     1621 ctggccgccc gcttccagcg tcagcagcgt atcatggttc accatgaaca cgtcgtcccc
     1681 cttgcccgct gcgagacgtt tttccagagc gacatagtag tcgttgcctt ttaagctctc
     1741 atacgacact cggatatcgg ggttttccac cataaaatcg gagatgatct cctcaatcac
     1801 cctcacattt tcaggctcgt atttgttccc gaaaaaagtg atggtcgtga tctcgcctga
     1861 gatttccggc tcgacagaga ccacattctt ggccccgcag ccggaaaatg ccaaagcggc
     1921 ggcaaggcat attgccgtcc gcttgatccc tttgctcttc atctgcgtct gcgtcctcct
     1981 cttgatgtcc ggggcccttt gggtatggcg gcacaaagcg ctgccatggc cgtccgccgc
     2041 ccctcggcct ttcctgccgc ctgcgcacgg cacaggctgc gcggtgcttt tcccctgcag
     2101 ccttgaaagg cttgcctctc ccccccgcgg cggtcccgac ggagcggccc tgacctgcgc
     2161 ttctgcttac aatattttcc tgggcgccgc ggcaattttg atctcttttt cagctgtttt
     2221 gtgctcccat gctttcagaa ttctaatctg cgaaaagcct gaacccgccc aaaagaaagc
     2281 ggcctcttgt ttccttgcat cgccgaagta ctctgtccat atcattcaca gataatttta
     2341 ccacgcatgc tttcaaaacg caaactttgc atgcgggcca agaggcctgg ccctagcaag
     2401 acggacaatg tatccgcatg tcttttatca ggtgagagga cttaaataaa aaaggaaacg
     2461 cgggatttcc gcgtttcctt ttttatcctc tgagccgccg gcgggctttc cgcccccgca
     2521 agccgctgtt tttcaaaccg aagctgccgt ctcccggttc tgcgactgct cagatcgcgg
     2581 tccgggcgct gtcgatgcgc tccagtcccc aggtgcgcca tgcccaaagc gtttcttcgc
     2641 cctggcgggt
//
LOCUS       DBMDYA010000417         2645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8460_length_2645_cov_1.431211, whole genome shotgun sequence.
ACCESSION   DBMDYA010000417 DBMDYA010000000
VERSION     DBMDYA010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_2645_cov_1.431211"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(348..1196)
                     /locus_tag="ACZPFK_09965"
     CDS             complement(348..1196)
                     /locus_tag="ACZPFK_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1406681.1"
                     /translation="MPIQILADSCCDLTPALKSLLGLRHIPLELRPGLGQSFVDDETL
                     DTHLLLQAMKATKEATGTACPSPEAYAQAMRGCDECVVITLSAKLSGSYNAAVMGRDL
                     VLEEEPEKKVFILDSESAAAGETRLALLLFDLIRAGLPFDEVVAQACAFRDKMRTLFV
                     LEDLSNLIKNGRISRAAGVIGSMLSLRPIMSDNGHGEIACLEKVRGTANALAKLVVRV
                     SEMTRDAAAHSVQLVMSQCNCTERALGLKKALLEACPALEDVLIVPAAGVSTVYANDG
                     GIVIAF"
     gene            1405..1884
                     /locus_tag="ACZPFK_09970"
     CDS             1405..1884
                     /locus_tag="ACZPFK_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR1406682.1"
                     /translation="MENRVTEFFKQKGFDLQAIFTGEDTSTVAKAAAVLGVEDGMIAK
                     SLAFRLKDGTPIVLVVCGTGRIDNRKFKEQFHCKASMLSFEDTLAVTGHPVGGVCPFA
                     LPKGVGVYLDKSLKKYQVVYPAAGTPDSAVRVEVARFEEYTGGVWVDVCKEEAPAAE"
     gene            2150..>2645
                     /locus_tag="ACZPFK_09975"
     CDS             2150..>2645
                     /locus_tag="ACZPFK_09975"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1406683.1"
                     /translation="MKTDIEIAQGTELLPVARIAEAVGLPEESVSLYGRYKAKVDHRL
                     VARSEQPDGRVILVTAISPTPAGEGKTTTSVGLADALHRLGRRVMLCLREPSLGPVFG
                     VKGGAAGGGYAQVVPMEDINLHFTGDLHAIGAANNLLAAMIDNHIYQGNALGIDPRRV
                     LFKRC"
BASE COUNT          531 a          797 c          821 g          496 t
ORIGIN      
        1 gttttgcttt tgatatcagg atcgccttta cctccaccga ttccacaatc gtcggcccaa
       61 aaatcatccc aatacccagg gataccaatg gtgaaatcaa aattgatatc aatttcttgg
      121 agattcttca tagcagacat ttctttcttt ggcgattttg ttagtcaagc ctctttaatc
      181 tactcattat tttccccgtt ttaaaacgcg gtcacgatcc ctacgccagt gggttcgtcg
      241 atggttgcac ccatacacag atgatagtgc gaactccggt gaggccgcct tgcggcggcc
      301 tcccaagctc agagcaagcc atcccgcaaa tgatgagttt tggcttttca aaacgcgatg
      361 acaatgcctc catcatttgc gtagacggtg gataccccgg ccgcggggac gatcagcacg
      421 tcctccagcg ccgggcaggc ctccagcagc gcctttttca ggccaagcgc ccgctcagtg
      481 cagttgcatt ggctcatcac cagctgcacg ctgtgggccg ccgcgtcccg ggtcatctcg
      541 ctcacccgga ccaccagctt ggcaagtgcg ttggcggtgc cgcgcacctt ttccaggcag
      601 gcgatttcgc cgtggccgtt gtcgctcatg atgggccgca ggctgagcat actgccgatc
      661 acacccgccg ccctgctgat gcggccgttc ttgatcaggt tcgacaggtc ctccagcaca
      721 aacagggtgc gcattttgtc ccgaaaagcg caggcctgcg ccaccacctc gtcaaagggc
      781 agcccggccc ggatgaggtc gaacaacagc agggccagcc gggtctcgcc cgccgccgcg
      841 ctctcggaat ccaggatgaa gacctttttc tccggctctt cctccagcac cagatcgcgc
      901 cccatcacgg cggcgttgta gctgccggac agcttggcgg acagggtgat caccacgcac
      961 tcgtcgcatc cgcgcatggc ctgcgcgtag gcctcggggg aggggcaggc ggtgccggta
     1021 gcctcctttg tggctttcat ggcctgcagc agcagatggg tgtccagcgt ctcgtcgtcg
     1081 acaaaggact gtcccaggcc ggggcgcagc tccagcggga tatggcgcag ccccagcagg
     1141 cttttgagcg cgggggtcag gtcgcagcag ctgtcggcaa ggatctggat tggcataaca
     1201 gggggcctcc ttcggccgca gagggccggt ctttcggttg gatacgagtt ccattgtaag
     1261 gattgccgcc ggttttgtca aacgaaacag ggcggatgga cccggcgggc aatacttgta
     1321 attatacttt ggatttgtta atattctgta tatacggcaa aaaaggtttg cgcgccgttg
     1381 ccgcaggaga ggggaaagag aaaagtggag aacagagtga ccgaattttt caaacagaaa
     1441 ggctttgacc tgcaagcgat ctttaccggc gaggacacca gcaccgtggc caaggcggcc
     1501 gccgtgctgg gggtagagga cggcatgatc gccaagagcc tcgctttccg cctcaaggac
     1561 ggcaccccca tcgtgctggt ggtctgcggc accggccgca tcgacaaccg caagttcaaa
     1621 gagcagttcc actgcaaggc cagcatgctc agctttgagg acacactggc cgtcactggg
     1681 catccggtgg gcggggtctg cccctttgcc ctgcccaagg gggtgggcgt ttacctggac
     1741 aagtcgctga aaaaatatca ggtggtctac cccgcggcgg gcacgcccga cagcgcggtg
     1801 cgggtcgagg tggcgcggtt tgaagagtac accggcggcg tctgggtgga cgtctgcaag
     1861 gaggaagcgc ccgccgcgga ataggatgga aaaaggccgg gccccccgcg cgggaggatg
     1921 cccggttttt tcgcgccgcg aggcgcggcg gagaagaaca caccgtgacg acagaggacg
     1981 gttgacaaac caagagccgc agaaaacgca ggcgattttt ttggcaggcg aaacaaaaaa
     2041 cgcaggcaag cctttgtggc tgaacgagta cttttgtgaa atacgccgca ataaagcgtc
     2101 catgttttca gcgggcgcgt ttgtcaacag ccccgacagg aggcaaagca tgaaaactga
     2161 tatcgagatc gcccagggca ccgagctgct gcccgtggcc cgcatcgcgg aggccgtggg
     2221 cctgccggag gagagcgtca gcctctacgg ccgctataag gcaaaggtgg accaccgcct
     2281 ggtggcccgc agcgagcagc cggacggccg ggtcatcctg gtcaccgcca tcagccccac
     2341 cccggcggga gagggcaaaa ccaccaccag cgtgggcctg gcggatgcgc tgcaccggct
     2401 gggcaggcgg gtgatgctct gcctgcgcga gcccagtctg gggccggtgt tcggcgtcaa
     2461 gggcggcgcc gcgggcggcg gctatgccca ggtggtgccc atggaggaca tcaacctgca
     2521 cttcaccggc gacctgcatg ccatcggcgc ggccaacaac ctgttggccg ccatgatcga
     2581 caaccatatc tatcagggca atgcgctggg catcgacccg cgccgcgtcc tctttaaacg
     2641 ctgca
//
LOCUS       DBMDYA010000418         2644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8465_length_2644_cov_1.797877, whole genome shotgun sequence.
ACCESSION   DBMDYA010000418 DBMDYA010000000
VERSION     DBMDYA010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8465_length_2644_cov_1.797877"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /gene="ptsP"
                     /locus_tag="ACZPFK_09980"
     CDS             <1..1299
                     /gene="ptsP"
                     /locus_tag="ACZPFK_09980"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1406684.1"
                     /translation="YTAKMRSIPDEYLSQRAGDIQDACRRVVDILDGRPRERMTVDAP
                     CILVSDELLPSDIVSVDHSLLLGVVTAGGSAQSHASIIARTFGIPAVVLAGPQVLTVP
                     DGTPCALDGAGGLLVLEPDEPTYARYAHRINLAKRRRVSQERLRTTPCISRDGVPISL
                     LANCSGPTDITRALELGADGVGLVRSEFLFLDGALPDEACQTAFYRDCVRAAQGRPIT
                     IRTLDIGADKEVAGLTGEEPNPALGLRGLRFSLAHSELFHTQLAALLKAGLEGPLKVM
                     FPMVASLQDLERALAAVEEVQRTLTLRGEAFSKNVEFGIMIETPAAALMADELAARAA
                     FFSVGTNDLTQYTHAADRVNPAVEQYYTPASPAVLRLLRFVARSAADAGIEVSVCGES
                     AADPELALLYARAGIRKLSMAAPSIAEVKERLLDETISIG"
     gene            1367..2239
                     /locus_tag="ACZPFK_09985"
     CDS             1367..2239
                     /locus_tag="ACZPFK_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1406685.1"
                     /translation="MSNYVIITDSTGDLSAELVREMEVEVIPMEFTVGDKPYKNYPDN
                     HDLSAQEFYARLRQGAAATTAQVNVTTYVETATPFLQAGKDVLMLIFSSGLSGSFNSS
                     CLAAEELRAKFPGRTVRTVDTLAASMGEGLLVWHACQLRKAGKTLDEVAAWAEANRNR
                     LCHWFTVDDLHFLRRGGRVSGAAAVMGTMLNIKPVLHVDDEGHLIPMEKVRGRRQSLD
                     ALVKHLDETIAEGENGTVFLSHGDCREDAEYVAEKIRGRHGIERVEINFIGPVIGSHS
                     GPGTVALFWLGSHK"
     gene            complement(2408..>2644)
                     /locus_tag="ACZPFK_09990"
     CDS             complement(2408..>2644)
                     /locus_tag="ACZPFK_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase family protein"
                     /protein_id="HIR1406686.1"
                     /translation="KSRMWKQYGMLCSEQEGAAFYTAAAQFGCRALMMVGITTGIRYD
                     ENGNEMFVELPDRTPAHTMDDLVQLALKTAAHAE"
BASE COUNT          446 a          881 c          890 g          427 t
ORIGIN      
        1 tacacggcca agatgcgcag catcccggac gaatacctgt cacagcgggc cggggatatc
       61 caggacgcct gccgcagggt ggtggacatt ctcgacggcc gcccgcggga gcggatgacc
      121 gtggacgcgc cctgcatcct ggtgtccgac gagctgctgc ccagcgacat cgtatcagtg
      181 gatcacagcc tgctgctggg cgtcgtcacg gcgggcggct ccgcccagag ccacgcctcc
      241 atcatcgccc gcacgttcgg catccccgcc gtggtgctgg ccggcccgca ggtgctcacc
      301 gtgccggacg gcaccccctg tgccctggac ggcgcgggcg ggctgctggt gctggagccg
      361 gacgagccga cctacgcccg ctatgcccac cgcatcaatc tggccaagcg ccgccgcgtc
      421 tcgcaggagc gtctgcgcac caccccctgc atcagccgcg acggggtccc gatcagcctg
      481 ctggccaatt gcagcggccc cacagacatc acccgcgcgc tggagctggg cgcggacggg
      541 gtgggcctgg tgcgcagcga atttctgttt ttggacggcg cgctgccgga cgaagcgtgc
      601 cagaccgctt tttaccgcga ctgtgtgcgc gccgcccaag gccgccccat caccatccgc
      661 accctggaca tcggcgcgga caaggaggtc gccggcctca ccggggagga gcccaacccc
      721 gccctggggc tgcgggggct gcgcttttct ctggcgcaca gcgagctgtt ccacacccag
      781 ctggcggcgc tgctgaaagc cgggctggag ggcccgctga aagtgatgtt ccccatggtg
      841 gcctcgctgc aggatctgga gcgggcgctg gccgccgtgg aggaggtgca gcggacgctc
      901 accctgcgcg gggaggcctt ttcaaaaaac gtcgaatttg gtattatgat agagaccccg
      961 gccgccgccc tgatggcgga tgagctggcc gcccgcgccg cttttttcag cgtgggcacc
     1021 aacgacctga cccagtacac ccatgcggcc gaccgggtca accccgcggt ggagcagtat
     1081 tacacccccg catcccccgc cgtgctgcgc ttgctgcggt ttgtggcccg cagcgccgcc
     1141 gacgcgggca tcgaggtttc cgtctgcggc gagtccgccg ccgacccgga gctggcgctg
     1201 ctctatgcgc gcgcgggcat ccgcaagctt tcaatggcgg ctccctcgat cgcggaggtc
     1261 aaagagcggc tgctggacga gaccatctcg atcgggtaga ccgggcgggg gcggccccgc
     1321 tgcgggcaaa aaaagccggg tccggcccgg cggtaaggag cgtttcatgt cgaattatgt
     1381 gatcatcacc gattccaccg gtgatctctc cgccgaactc gtgcgcgaga tggaggtgga
     1441 ggtcatcccg atggagttca ccgttgggga caagccctac aagaattatc ccgacaacca
     1501 cgacctctcc gcacaggagt tttacgcccg actgcggcag ggggcggcgg ccaccacggc
     1561 ccaggtgaac gtgacgacct atgtggagac cgcgacgcca tttttacagg cgggcaagga
     1621 cgtgctgatg ctgatcttct cctccggtct ctcgggcagc ttcaactcca gctgcctcgc
     1681 ggcggaggag ctgcgggcca agttccccgg ccgcacggtg cgcacagtgg acaccctggc
     1741 cgccagcatg ggcgagggcc tgctggtctg gcacgcctgc caactgcgca aggcgggcaa
     1801 gacgctggac gaggttgccg cctgggccga ggccaaccgc aaccggctgt gccactggtt
     1861 cacggtggac gacctgcact ttctgcgcag gggcggccgg gtgagcggcg ccgcggccgt
     1921 gatgggcacg atgctgaaca tcaagccggt gctgcatgtg gacgacgagg ggcatctgat
     1981 ccccatggaa aaggtgcgcg gccgccggca gagcctggac gcactggtca agcatctgga
     2041 cgagaccatc gcggagggcg agaacggcac tgtttttctc agccacggcg actgccgcga
     2101 ggacgcggaa tatgtggccg aaaagatccg cggccgccac ggcatcgagc gggtggagat
     2161 caacttcatc ggcccggtga tcggcagcca ttccggcccc ggcacggtgg cgctgttctg
     2221 gctgggcagc cacaaataaa agcgcccggc gcccggtaaa agaggctttt tcggccatcc
     2281 ggcccggctt gccatggcca aaaaggaaac ataaaaacac ccggcaaagg ccgggtgttt
     2341 tttgattcaa atacaaaagt cgcacaaagg ccctatgcgg ccctgccgtt cgccacctgc
     2401 ggccgcttta ttccgcgtgg gcggcggtct tgagggccag ctgtaccaga tcgtccatgg
     2461 tgtgggcggg ggtgcggtcc ggcagctcca caaacatctc gttgccgttc tcgtcgtagc
     2521 ggatgccggt ggtgatgccc accatcatca gggcgcggca gccgaactgg gccgcggcgg
     2581 tgtaaaaggc ggcgccctcc tgctcagagc agagcatgcc gtactgcttc cacatcctgg
     2641 actt
//
LOCUS       DBMDYA010000419         2643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8470_length_2643_cov_1.308419, whole genome shotgun sequence.
ACCESSION   DBMDYA010000419 DBMDYA010000000
VERSION     DBMDYA010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8470_length_2643_cov_1.308419"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(512..>819)
                     /locus_tag="ACZPFK_09995"
     CDS             complement(512..>819)
                     /locus_tag="ACZPFK_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1406687.1"
                     /translation="PTRFQHLVAGTYKKWAGENGVKYFSVASGGGTGRTLHPDNVAAG
                     PASYGLTDSMGRMHGDAQFAGSSSVPAHVEMMGLMGMGNNPMVGATVACAVAVSEAK"
     assembly_gap    820..829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(830..1496)
                     /locus_tag="ACZPFK_10000"
                     /pseudo
     CDS             complement(830..1496)
                     /locus_tag="ACZPFK_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
     gene            complement(1515..2207)
                     /locus_tag="ACZPFK_10005"
     CDS             complement(1515..2207)
                     /locus_tag="ACZPFK_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1406688.1"
                     /translation="MTYSQEVERMCTVAKGVHHGPAPIPEEGEWVKAYEIKDISGLTH
                     GVGWCAPQQGACKLTLNVKDGIIQEALVETLGCSGMTHSAAMAGEILPGKTLLEALNT
                     DLVCDAINVAMRELFLQIVYGRSQTAFSEGGLPIGAGLEDLGKGLRSQVGTIFSTGAK
                     GVRYLEMAEGYILSIALDDEDHTIGYKFVRVGKMLEDIRHGVDAKEAYEKNVGTYGRY
                     DGAAKYIDPREE"
     gene            complement(2383..>2643)
                     /locus_tag="ACZPFK_10010"
     CDS             complement(2383..>2643)
                     /locus_tag="ACZPFK_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1406689.1"
                     /translation="KGTVARTAAALEAQGLLLRSPDPADRRVNRVSLSPRGQALLPRV
                     EAVIDRLHAVIYRGLTQQEQDAACRLLVQMCDNLRGALGGAR"
BASE COUNT          411 a          892 c          854 g          476 t
ORIGIN      
        1 cccaccccgc aaaaaagagg cggcgcggaa aaaccgtacc agggaggtga cggatggaga
       61 ggcgaaccgc aaagtgagga aaatgaccgc tcggcggcaa ggacggcact gccaccccca
      121 gacggcctgc ggccgccaac ttcccggcca aggggaaacg gcacggtaaa actgcggtgg
      181 ggaactggcg cgcgctgtcg cagccggcgg gcgagggcgg gaacgccgct attttccgca
      241 aaacacaaaa gcctgaggcg cgcgcaggtt gtcgcccccg ggcaggcctc cgagctgtgc
      301 cgccgcaggc tgggggcggg gtcgggctgc gttctccgcc gcccctccgg caaaaggcgc
      361 cgggcggcgc tttgccccgc gtcgggcttg gcgcatcgtc cccatcgccg gcctccagcc
      421 cgcaaagcgc tggaaaaggc ggcaaagagg gccgctcctg ccggagcggc cctctttttt
      481 tcgtctcaca gcctcacgga tgcaagcggg cttatttcgc ctcgctgacg gccacggcgc
      541 aggccacggt cgcgccgacc atggggttgt tgcccatgcc catcaggccc atcatctcca
      601 cgtgcgcggg cacggaggag gagccggcga actgggcgtc gccgtgcatg cggcccatcg
      661 aatcggtcag gccgtagctg gccgggccgg cggccacgtt gtccgggtgc agggtgcggc
      721 cggtgccgcc gcccgaggcg accgagaagt acttgacccc gttctcaccg gcccactttt
      781 tgtaggtgcc ggccaccaga tgctggaagc gggtcgggtn nnnnnnnnna gatgtccacc
      841 tgctcttttt tcatgatggc gacgccctcg agcacatcgt tggcgccgta gcacttgacc
      901 gcggcgcggt cgccgtcgga gtacttgatc tcctcgacca ccttcaggtc gccggtcttg
      961 aagtcgtact cggtcttgac ataggtgaag ccgttgatgc ggctgatgat ctgggcggcg
     1021 tccttgccca ggccgttcag gatgacgcgc agcggctctt tgcgggcctt gttggcggtg
     1081 cgggcgatgc cgatggcgcc ctcggccgcg gcaaagctct cgtgaccggc caggaacgcg
     1141 aagcatttgg tctcgtcgcg cagcagcatg gcgcccaggt tgccgtggcc caggccgacc
     1201 tggcgctgct cggccaccga gccgggcacg cagaacgcct gcaggccgat gccgatggcc
     1261 tcggccgcgt cggcggcgct ggtgacgccc tttttcagcg ccacggcgca gcccagggtg
     1321 tacgcccaga cggcgttgtc aaacgcgatg ggctgcacgc ccttgacgat cttctcgcag
     1381 tccacgccct tggacaggca gaggtcgcgg gcggcctcca gatcagcaat gccgtactct
     1441 ttcaggcagg cattgatctt ttccattctt ctctctttac cttcgaaatt gaccatcttt
     1501 gagtaccccc tttcttattc ctctcgcgga tcgatgtatt tggcggcgcc gtcatagcgg
     1561 ccataggtgc cgacgttctt ctcgtaggct tccttggcgt ccacgccgtg gcggatatcc
     1621 tccagcatct tgcccacgcg cacaaacttg tagccgatgg tgtgatcctc gtcgtccagc
     1681 gcgatggaga ggatatagcc ctcggccatc tccagataac gcacgccctt ggcgccggtg
     1741 gagaagatgg tgcccacctg gctgcgcagg cccttgccca ggtcctccag gccggcgccg
     1801 atgggcaggc cgccctcgga gaacgcggtc tggctgcggc cgtagacgat ctgcaaaaac
     1861 agctcgcgca tggccacgtt gatggcgtcg cagaccaggt cggtgttcag ggcctccagc
     1921 agggtcttgc cgggcaggat ctcgccggcc atggcggccg aatgggtcat gccggagcag
     1981 cccagcgtct ccaccagggc ctcctggatg atgccgtcct tgacgttcag ggtcagcttg
     2041 caggcgccct gctgcggcgc gcaccagccc acgccgtggg tcagaccgga aatgtctttg
     2101 atctcatacg cttttaccca ctcgccctcc tcggggatcg gggccggacc atgatgtaca
     2161 cccttggcca cagtgcacat gcgttctact tcttgcgaat aggtcatagt agcactcctt
     2221 catctttttc cgggacggcg agccgcccct ttttcccgct tgcatcggaa cacctttatt
     2281 ataaaggccg ccggggtaaa cttcaagact ccggccgcca tttcgggcgc tttcgtttaa
     2341 aatttaacag ttgccgcccc ggcgcggggc cgggccgccg cgtcacctgg cgccgcccag
     2401 cgcccctctc aggttgtcgc acatctgcac caacaggcgg caggcggcgt cctgctcctg
     2461 ctgcgtcaag ccgcggtaga tcaccgcgtg cagccggtcg atcaccgctt ccacccgcgg
     2521 cagcagcgcc tgcccccggg gggacagtga gacgcggttc acccgccggt cggcggggtc
     2581 ggggctgcgc agcagcaggc cctgggcctc cagcgccgcc gcggtgcggg ccacggtgcc
     2641 ctt
//
LOCUS       DBMDYA010000420         2636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8496_length_2636_cov_2.018146, whole genome shotgun sequence.
ACCESSION   DBMDYA010000420 DBMDYA010000000
VERSION     DBMDYA010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_2636_cov_2.018146"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            116..697
                     /gene="pgsA"
                     /locus_tag="ACZPFK_10015"
     CDS             116..697
                     /gene="pgsA"
                     /locus_tag="ACZPFK_10015"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1406690.1"
                     /translation="MNLPNKLTLARMALVPVFMVFAALGQYGTPSFNSAWYLVAGIVF
                     AAASFTDFLDGHLARKNHMVTDFGKFADPLADKLLTTVAFLYMMRDGLCSPVVLAVIL
                     AREFAVSGLRMVAAGAKDGRVIAANIWGKVKTVLQMLTIIFYYFAAALAGPTGVMAVG
                     LITQALCWLVALVTAASGIQYLWNNRQFINTAK"
     gene            726..1625
                     /locus_tag="ACZPFK_10020"
     CDS             726..1625
                     /locus_tag="ACZPFK_10020"
                     /inference="COORDINATES: protein motif:HMM:NF022337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HIR1406691.1"
                     /translation="MKCQVCGAESGKYPLCRSCNQKRSTGEVIKCAKCNTWHYANAPC
                     PFSTVEEPQCGPFLYKAKSSLVTKNELGYLNCIKSVLPDGYQVHVQANLASFISRTDG
                     AKFQNELYRNVDFLITNLSYQPLIAIEINDQTHLTQERRERDKKVLQICEEAGIPMIR
                     LWTSYGVNPDYIQKRITETIASLPVKRISHFSKEIKTDVPNATSFLSPQKKGCYIATC
                     VYGSYDCPQVWTLRRYRDQYLATTLHGKLFIKLYYALSPFFVKILGKRKFFKAFWKRA
                     LDSFIVKLNQQGIPGIPYKDSAE"
     misc_binding    1698..1946
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2083..>2636
                     /locus_tag="ACZPFK_10025"
     CDS             2083..>2636
                     /locus_tag="ACZPFK_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1406692.1"
                     /translation="MNIPHRLYLTEDQMPRQWYNLRADLPCQPDPMLDPETLRPVSEK
                     DLYPIFCQELARQELDARSRYVDIPEGILEMYRIYRPSPLCRAYQLEKALDTPAHIYY
                     KFEGNNTSGSHKLNSAIAQAYYAKEQGLSSLTTETGAGQWGTALAEACAHFGLDLTVF
                     MVKVSYEQKPFRKAVMQTFGADVTA"
BASE COUNT          605 a          758 c          692 g          581 t
ORIGIN      
        1 tggtctatgt ggattccccc tccgtcccgc tgcaggaggg cggcttttac agcgtgcggg
       61 tcgacgacag cgacgtcttt gacctgtacg ctcatctgga agagggggaa acgcgatgaa
      121 tctgcccaac aagctcactc tggcccgcat ggctctggtg ccggtgttta tggttttcgc
      181 cgccctgggg cagtacggca cgcccagctt taacagcgcc tggtatctgg tggccggcat
      241 cgtcttcgcc gccgccagct tcaccgactt tttagacgga catctggccc gcaagaacca
      301 catggtcacg gacttcggca aatttgccga cccgctggcc gacaagctgc tcaccacagt
      361 ggcttttttg tacatgatgc gggacggcct gtgctcgccg gtggtgctgg ccgtcatcct
      421 ggcgcgggag ttcgcggtgt cgggcctgcg catggtggcc gccggggcca aggacggccg
      481 cgtcattgcc gccaatatct ggggcaaggt caaaaccgtg ctgcagatgc tcaccatcat
      541 cttctattat tttgccgccg cgctcgccgg ccccaccggc gtgatggccg tcggcctcat
      601 cacccaggcg ctctgctggc tggtggccct cgtcaccgcc gcctccggca tccagtatct
      661 ctggaacaac cgccagttca tcaacacggc aaagtaacgc cctaaaggga ataaggaaaa
      721 gcactatgaa atgtcaagtc tgcggagctg aaagcggcaa atatcctctt tgcagaagtt
      781 gtaatcaaaa gagaagcacc ggtgaagtga taaagtgtgc taaatgtaac acatggcatt
      841 atgccaatgc tccctgtcct ttctctacag tggaagaacc ccagtgtggg ccttttctct
      901 ataaagcgaa atcttcttta gtcacaaaaa acgagttggg atatcttaac tgcatcaaat
      961 ctgttttgcc cgacggatac caggtacacg tgcaggccaa tctggcctct ttcatttcgc
     1021 ggacagatgg ggccaaattt caaaatgaac tctatcgcaa tgttgacttt cttattacaa
     1081 atctctctta ccagcccttg atcgcgatcg agatcaacga ccagacccat ctcacgcagg
     1141 aacggcggga aagggataaa aaagttttgc aaatctgtga agaagccggt attcccatga
     1201 tccgcctttg gacttcttat ggggtgaatc ccgattatat tcaaaagcgc atcaccgaga
     1261 cgatcgcttc tctccctgta aaaagaatct ctcacttttc aaaagagatt aaaacggatg
     1321 tcccaaatgc gacgtctttc ctttccccac agaaaaaagg atgttacatt gccacctgtg
     1381 tatacggttc ttatgactgc ccccaagtgt ggacactccg gcgctatcgc gaccagtatc
     1441 tagccacgac tttgcacgga aaacttttta tcaagcttta ctatgctctc agcccctttt
     1501 ttgtaaaaat actggggaag agaaaatttt ttaaagcttt ctggaagcgg gctttagact
     1561 cgttcatcgt caaactcaat caacaaggca tcccaggaat cccctataaa gactccgcag
     1621 aatagttaaa acagaaaggt gtctttttca aaaaacggtt gacacctcgc cctggaagcg
     1681 ctataatagc gccatattcc aaaccgttga ataagaagag taggctttca agacgctttc
     1741 agagaaccgt tgggtggtgc gaaacggcaa gcggcttttc agctgaatgg acttatgagg
     1801 gcaggacgaa cggccgcacg cccagtagtg cctgacggga tcttcacccg ttatcaagga
     1861 agcgcatatg atcgtatgcg gagcgagtgg gcgcatttgc gccaatcagg gtggtaccgc
     1921 agaagcacaa ggcttttgtc ccggtttttt tgccggggca aaggcctttt tgttttgccc
     1981 cggcagcccg ggcttcagag ccgcttttgt ccctgggtcg atatgcgccg acgcgccccc
     2041 ctcggcgggc ccgtttgcag ataaatagaa gaggtgaacg agatgaacat tccgcacaga
     2101 ctgtatttga ccgaagacca gatgccccgc cagtggtata acctgcgggc cgacctgccc
     2161 tgccagcccg acccgatgct ggacccggag acgctgcggc ccgtctcaga gaaagacctg
     2221 taccccatct tctgccaaga gctggcccgc caggagctgg acgcccgcag ccgctacgtg
     2281 gacatcccgg aggggattct ggagatgtac cgcatctacc gcccctcccc cctctgccgc
     2341 gcctaccagc tggaaaaggc gctggacacc ccggcccaca tctattacaa attcgagggc
     2401 aacaacacct cgggcagcca caagctgaac tctgccatcg cccaggccta ttatgccaag
     2461 gagcagggcc tctccagcct gaccaccgag accggggcgg gccagtgggg cacggcgttg
     2521 gcggaggcct gcgcccattt cgggctggac ctgaccgtct ttatggtcaa ggtctcctac
     2581 gagcaaaagc ccttccgcaa ggcggtgatg cagacctttg gggcggatgt gaccgc
//
LOCUS       DBMDYA010000421         2629 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8522_length_2629_cov_1.524921, whole genome shotgun sequence.
ACCESSION   DBMDYA010000421 DBMDYA010000000
VERSION     DBMDYA010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8522_length_2629_cov_1.524921"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            13..141
                     /locus_tag="ACZPFK_10030"
     CDS             13..141
                     /locus_tag="ACZPFK_10030"
                     /inference="COORDINATES: protein motif:HMM:NF018401.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HIR1406693.1"
                     /translation="MCADAGQSAVAGRVVLAGKVAIVAVTLPLFQSLVELLAELLR"
     gene            249..1322
                     /locus_tag="ACZPFK_10035"
     CDS             249..1322
                     /locus_tag="ACZPFK_10035"
                     /inference="COORDINATES: protein motif:HMM:NF021088.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR1406694.1"
                     /translation="MKKIVSLLVLLLVLALPVSAASQGEEEDGLPQQVTEFLDKYGDG
                     ARQLQELTPQSLWDALLRAAKEQLQRPLKMFYQLAAVLVLAALAKAVTADWANMQVGR
                     QVDMAVILASFLFACRPLLQLLAEIGEMILECKTFVLSFVPVFATVMATCGQPAAGAV
                     YTGFFLSSVVVAATLITGVLLPFMKIYMALNISGGLSSTVDLSGLAAMVSKFIKWGLG
                     LIATVFGAVIGLQSLLAGAADSVALKAGKFLIGSSVPVIGGAVSDAIGTVYAGLKVVK
                     GTAGAAGICAIVVLFAPLLLRCLAYYLSLSLAAAAATVTGNGRAGKVFGGFCECLELY
                     ISILIFFAVMIILSTALMISLGN"
     gene            1340..1819
                     /locus_tag="ACZPFK_10040"
     CDS             1340..1819
                     /locus_tag="ACZPFK_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406695.1"
                     /translation="MELIRRWVLVLCVSAVLASVLQSVLPEKGSFSVIKLVLSLYILI
                     TLISPVREFSAADLSLPLEQAQTAYVQADLTESILGQAEQKLEATVRRGLQTAGLDPG
                     AVSVQLALDESGEATLAGVTVACDGDGEMIREAVDGALGCEAPLALLPRNGMEETWN"
     gene            1810..2361
                     /locus_tag="ACZPFK_10045"
     CDS             1810..2361
                     /locus_tag="ACZPFK_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406696.1"
                     /translation="MELKKMWERFKAPEGKKKRISALFLLGLAGILLIFLSDLTGQKT
                     KTPAETEGENLELYTQKLEARLLDTVEVIQGVGKVKIMLTLEGGAETVYASTEKSAKT
                     QTVSDSGANSSQQSSYENELVLVDAGSGRQALVETAREPEIKGVAIVCEGGDDVAVIK
                     RITDVVSVVLGIPTNRICVTKMT"
     gene            2376..>2629
                     /locus_tag="ACZPFK_10050"
     CDS             2376..>2629
                     /locus_tag="ACZPFK_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406697.1"
                     /translation="MKSMLNRKKITLLTLVVALGVAVYLNWEYAKTGSSDFGLTAGSS
                     ASTVQTAAQPAGDAGLEVQGEVSSQPPVADKNYGDAQLVSL"
BASE COUNT          481 a          729 c          919 g          500 t
ORIGIN      
        1 ggggcggcgg atatctgcgc cgacgccggg cagagcgccg tggccgggcg ggtggtgctg
       61 gcgggcaagg tggccatcgt ggcggtgacc ctgccgctgt ttcaaagcct ggtcgagctg
      121 ttggcggagc tgctgcggta aaaagggcgg gcgcaggctg aacgtcccgc ctttccgggc
      181 gagggcgcgc gtttcgcaaa gagcttttgg ggccgcggcg gggctgcaaa tttttgagga
      241 gcaaaaggat gaaaaaaata gtgtcgctgc tggttctgct gctggtgctg gcgcttcccg
      301 tatccgccgc ctcacagggg gaggaggagg acggcttgcc gcagcaggtg actgaatttc
      361 tggacaagta cggcgatggg gcccggcagc tgcaggagct gacgccgcaa agcctttggg
      421 acgccttgct cagggccgca aaagaacagc tgcaaaggcc gctgaagatg ttttaccagc
      481 tggctgcagt gctggtgctg gccgcgctgg ccaaggcggt gacggccgac tgggccaaca
      541 tgcaggtggg caggcaggtg gatatggcgg tgatcctcgc ctcctttctg ttcgcctgca
      601 ggccgctgct gcaattgctc gccgagatcg gcgagatgat cctggagtgc aagacctttg
      661 tcctcagctt tgtgcccgtc ttcgccaccg tgatggccac ctgcggccag ccggcggcgg
      721 gtgcggtgta cactggcttt ttcctctcca gcgtggtggt ggcggccacc ctgatcaccg
      781 gcgtgctgtt gccgttcatg aagatctata tggcgctcaa tatctccggc gggctctcct
      841 ccaccgtgga cctcagcggc ctggcggcca tggtctcaaa attcatcaag tgggggctgg
      901 gcctcatcgc caccgtgttc ggcgccgtga tcgggctgca gagccttttg gccggggcgg
      961 ccgacagcgt ggccctcaag gcgggcaaat ttttgatcgg cagcagcgtg ccggtgatcg
     1021 gcggcgcggt ctcggacgcc atcggcacgg tctacgccgg gctcaaggtg gtcaagggca
     1081 cggccggggc ggcgggcatc tgcgccattg tggttctgtt cgcccctctg ctgctgcgct
     1141 gccttgccta ctatctttcc ctcagcctcg ccgccgcggc cgccacggtg accggcaacg
     1201 gcagggcggg caaggtgttc gggggctttt gcgagtgcct ggagctgtat atctcgatcc
     1261 tgatcttctt tgcggtgatg atcatcctct ccacggcgtt gatgatctca ctgggaaatt
     1321 gagagaggag gcgacgcgga tggagctgat ccgcaggtgg gtattggtgc tctgcgtcag
     1381 cgcggtgctg gcctcggtgc tgcagagcgt gctgccggag aagggttctt tttctgtcat
     1441 aaagctggtg ctgtccctat atattttaat aaccctcatc tcgccggtca gggagttttc
     1501 cgccgcagac ctcagcctgc cgctggaaca ggcgcagacc gcttatgtgc aggcggatct
     1561 gacggaaagc atcctcggcc aggcggaaca gaaactggag gcaacggtca ggcgggggct
     1621 gcaaacagcc gggctcgacc ccggagccgt ctcggtgcaa ctcgcgctgg atgaaagcgg
     1681 agaggcgacc ctggccggtg tgaccgtggc gtgcgacggg gatggggaaa tgattcggga
     1741 ggcggtggac ggcgcgctgg gctgcgaggc gccgctggct ctcttgcccc ggaatggaat
     1801 ggaagagaca tggaactgaa aaagatgtgg gaacgcttca aagccccgga ggggaaaaag
     1861 aaaaggatca gcgccctgtt tcttctcggt ctggcgggca ttctgctgat ctttctctcc
     1921 gacctgacgg ggcagaagac aaagacgccg gccgagacgg agggcgaaaa cctggagctt
     1981 tacactcaaa agctggaggc gcggctgctg gacaccgttg aggtcattca gggcgtggga
     2041 aaggtaaaga tcatgctgac gctggagggc ggggccgaga cggtgtacgc ctccacggaa
     2101 aagagcgcaa agacgcagac agtttccgac tcgggcgcaa actccagcca gcagagcagc
     2161 tatgagaacg agcttgttct cgtggacgcc gggtccggca ggcaggcgct ggtggagacc
     2221 gcgcgggagc cggagatcaa aggggtggcc atcgtatgcg agggcggcga cgacgtcgcc
     2281 gtgatcaagc gcattaccga tgtcgtatcg gtagttctgg gcataccaac caaccgtatc
     2341 tgtgtaacaa aaatgacata acggaggaaa tatgtatgaa aagcatgctc aaccgaaaga
     2401 agatcaccct tttgaccctt gttgtcgcgc tcggcgttgc ggtctacctg aactgggaat
     2461 acgccaagac cggctcttct gattttggcc tgacggcggg ctcttccgcc agcacggtgc
     2521 agaccgccgc acagccggcg ggagacgccg ggctggaggt gcagggagag gtctccagcc
     2581 agccgccggt ggcggacaaa aactacggcg acgcccagct cgtttcgct
//
LOCUS       DBMDYA010000422         2628 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8523_length_2628_cov_1.667194, whole genome shotgun sequence.
ACCESSION   DBMDYA010000422 DBMDYA010000000
VERSION     DBMDYA010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_2628_cov_1.667194"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(341..1291)
                     /locus_tag="ACZPFK_10055"
     CDS             complement(341..1291)
                     /locus_tag="ACZPFK_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1406698.1"
                     /translation="MKYTIGIDLGGTNIVAGLVDEQYHLLDKAAVKTQPEKGFDFITD
                     NMARLVATLLQRAKLAPADVSSIGIGSPGTPDKETGDIIFSNNLNWHDVPLASSVQGK
                     TGIRTVVANDADCAALGEFVAGAGAEDSSMALITIGTGVGSGIVMDGKLFCPAGSNAP
                     ELGHTTLICGGEPCTCGRNGCAEAYISFSALIRDGNRAADAHPDSMLAKLRSEGQALN
                     GKNIFDCGKAGDPAAKELMDRFIEYMGQFLANVGNGFGVRAIVVGGGISKEGEWLCRR
                     LREIVVREMFVGEMFAPPIRMATLGNDAGVIGAAALERYR"
     gene            complement(1423..1569)
                     /locus_tag="ACZPFK_10060"
     CDS             complement(1423..1569)
                     /locus_tag="ACZPFK_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406699.1"
                     /translation="MIYVCENCKFLFERQGEVFHCPGCGSGHIRPADEEECQQYIKSK
                     ERAK"
     gene            complement(1819..>2628)
                     /locus_tag="ACZPFK_10065"
     CDS             complement(1819..>2628)
                     /locus_tag="ACZPFK_10065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1406700.1"
                     /translation="GEGYKGMSRGFTGAEDHAGVDLRADRGSPILAALDGTVADAGVN
                     ETDGAYVLLDHDGGMETFYAHCDELRVEKGQAVERGQTVATVGSTGQSTGNHCHFEVR
                     LDGVAADPAPYLEQRPQGEAPAEGTFAFPVGEGYTCLTRGFTGADGHGGLDLAAAYGT
                     PVFAAQSGVVTYAGQTRGGYGVHIVLDHGGGVTTLYGHCSALAVVEGQVVEQGQIIAY
                     VGSTGDSTANHCHFEISVNGAPVDPAVWFSGETRAAYGLTTGEDASEAQGQ"
BASE COUNT          412 a          933 c          781 g          502 t
ORIGIN      
        1 tcctttatcc tgcacctgcg gcaatggtgc ggctttccct cctcctcgct caagcagccc
       61 gcacacggct ttttagaggc acagtgctga tagcagacaa ggcagttcat tccgcaggga
      121 gcaaacagcc tctcctcgat cttttccgtt gacatcttca tccgccgtgt ccctttccgc
      181 ttccgcctgc gcggaagtct ccctgccgcc ggccacagcc gccgaaaaga gcgtcttttc
      241 gccgcggccg tcaaaaacaa ggagggggcg gcgagccgct ccctccaaag tcgtcccacg
      301 cgctgaaaac cgccgctttt tatctccccc cgcgccgggc tcagcgatac cgctccagcg
      361 cggcggcgcc gatgacgccc gcgtcgttgc ccagcgtggc catgcggatg ggcggcgcaa
      421 acatctcgcc cacgaacatc tcgcgcacca cgatctcgcg cagacggcgg cagagccact
      481 cgccctcttt gctgatgccg ccgccgacca cgatggcccg cacgccgaag ccgttgccca
      541 cgttggccaa aaactggccc atatactcga taaagcggtc catcagctct ttggcggccg
      601 gatcgcccgc cttgccgcag tcaaagatat tcttgccgtt gagggcttgt ccctcgctgc
      661 gcagcttggc cagcatgctg tcgggatggg catcggcggc gcggttgccg tcgcggatca
      721 gcgcgctgaa gctgatatac gcctccgcgc agccgttgcg gccgcaggtg cagggctcgc
      781 cgccgcagat cagggtggtg tgccccagct cgggcgcgtt ggagcccgcg gggcagaaca
      841 gcttgccgtc catgacgatg ccgctgccca cgccggtgcc gatggtgatc agcgccatgg
      901 aggagtcctc cgcgcccgcg cccgcgacaa actcgcccag cgccgcgcag tcggcgtcgt
      961 tcgccaccac ggtgcggatg ccggtcttgc cctgcacgga ggaggccagc ggcacatcgt
     1021 gccagttcaa attgttggag aagatgatgt ccccggtctc cttgtccggg gtgccggggc
     1081 tgccgatgcc gatgctgctc acgtcggcgg gggcgagctt tgcccgctgc agcagggtcg
     1141 ccaccagccg cgccatgttg tcggtgataa agtcaaagcc cttttccggc tgggtcttga
     1201 ccgccgcctt gtccagcaga tggtactgct cgtctaccag cccggccacg atgttggtgc
     1261 cgcccaggtc gatgccaatg gtgtatttca tcccgcaaaa ccccttatct ctcttgccgc
     1321 ctgtgcggcg gtcgttttct ggtcttattg taccacccgc ccccggcgcg cacaagccga
     1381 attttgcgct gcggcccccg caaacgcaca aaaaaagagg gcctatttgg ccctctcctt
     1441 gctctttatg tactgttggc actcctcttc gtcagcgggg cggatatggc cgctgccgca
     1501 gccgggacag tgaaacacct cgccctgccg ctcgaacagg aatttgcagt tctcgcagac
     1561 gtagatcaaa ctttgcttcc ccccgcttgg aacccatgtt tcatgataca actacaatta
     1621 taatggagcg aggcggaaag tcaagcagaa gcctgtcgat tattttcgcc gctttcttca
     1681 atttgcggct gttttgagac gttttttcgc gttttggcac agcagaaatt tccagctatc
     1741 cgcccttgcg gcgcacccac ccgtcccctt tcggggaaaa ggggcgcggc gcttgcccgg
     1801 ttctccgtcg ccccggcgct attggccctg ggcctccgaa gcgtcctccc cggtcgtcag
     1861 cccgtaggcg gcccgggtct cgccgctgaa ccagaccgcc gggtccaccg gcgcgccgtt
     1921 tacgctgatc tcaaaatggc agtggttggc ggtggagtct ccggtactgc ccacataggc
     1981 gatgatctgg ccctgttcca ccacctggcc ctccaccacc gcaagggcgc tgcagtggcc
     2041 gtaaagggtc gtcaccccgc cgccgtggtc aaggacgata tggacgccgt agccgccccg
     2101 agtctggccc gcataggtca ccacgccgct ctgggcggca aagaccgggg tgccgtaggc
     2161 cgcggccaga tcgaggccgc cgtgcccatc cgccccggtg aagccacggg tgagacatgt
     2221 gtagccctct cccaccggga aagcgaacgt cccctcggcc ggcgcttcgc cctgagggcg
     2281 ctgttccaga tagggggcgg ggtccgccgc cacgccgtcc aggcgcacct caaagtggca
     2341 gtggttgccg gtggactggc ccgtgctgcc cacggtggcg accgtctggc cccgctccac
     2401 cgcctggcct ttctccaccc gcagctcgtc gcagtgggcg taaaaggtct ccattccgcc
     2461 gtcatggtcg agcagcacat aggcgccgtc cgtctcgttc acgcccgcgt ccgccaccgt
     2521 cccgtcaagg gcggccagga tcgggctccc ccggtcggcg cgcaggtcca caccggcgtg
     2581 atcctccgcg ccggtaaagc cgcggctcat gcccttatat ccctcccc
//
LOCUS       DBMDYA010000423         2625 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8535_length_2625_cov_1.561410, whole genome shotgun sequence.
ACCESSION   DBMDYA010000423 DBMDYA010000000
VERSION     DBMDYA010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8535_length_2625_cov_1.561410"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="ACZPFK_10070"
     CDS             <1..361
                     /locus_tag="ACZPFK_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR1406701.1"
                     /translation="VEITDSVREIVAASGVRSGVAVVSTPDVDAGVLITSFYDPKGHE
                     DIIDDFVRIWPARDNFHFEGEVTEGAAHSKSSIAGQSVDFIVEDGELKLGGSQGIFFA
                     EYAAPRAREYRVAVFGA"
     gene            complement(451..1362)
                     /locus_tag="ACZPFK_10075"
     CDS             complement(451..1362)
                     /locus_tag="ACZPFK_10075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1406702.1"
                     /translation="MDYIAAGNVMSDIIEREDGSRSAMHLGGPAFFALAGIRLWTDSV
                     KLCSGVGADFEEIYGPWMRANRVPADAVSVRADHTTLHVLRYLENGKYAHESIYGAQN
                     LGYLKTRPEDLEAALDHTKGVYMAQNTDRVYWAKLARLKERHGFKFMLELEHNNGWED
                     VREICKLADYFSCNLNEASALLGIPREREEEIIAQLLRLPVEQIFFRVGARGSYALYG
                     NTATFVPAVDIGRPVDPTGCGNCSTGSAMYAYTEGFDPIMTAVMANIAAAYNVLQYGP
                     YPHFTEQTRREAVALAEKLRADYPPLP"
     gene            1458..>2625
                     /locus_tag="ACZPFK_10080"
     CDS             1458..>2625
                     /locus_tag="ACZPFK_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013473.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406703.1"
                     /translation="MHCPQDDRKLNRYFCLLQASIFCMMAIHSGFFSLMLSQQGVSKM
                     ELGRIAAVTGAASLVAQPLLGLISDRWRAERLLYAVGAVLAPLAYYNIQHTTRLGLIA
                     FWAALFVGFSLGAQILSTGWIAALNAAGHKLDFGFSRGVGSLSFALGSVVIGQAINRF
                     GMGCLPVLLAVFGLTLAAAAFLLPRPQRARPAQEADAPRAAGFGEAMHELVKTRPYVL
                     LVLCSFLGNLPSGAFTTYFSVFFSQEGGSASLLGIALFVLAIVEVPVMLNYRRLERRF
                     GVETLLVVSMLGYGLKNLCLALAPSLPFIVACLLLQAAGLALAIPASQSMIAKITDTA
                     CCATAQCLWSSLGEGLGMIASSLFCSWLVSFSSIRAVFAVTSLFGFAAAALFWAL"
BASE COUNT          377 a          832 c          895 g          521 t
ORIGIN      
        1 agtggagatc accgacagcg tccgcgagat cgtcgcggcc agcggcgtgc gcagcggcgt
       61 ggcggtggtc agcaccccgg acgtggacgc gggggtgctc atcacctcgt tctacgaccc
      121 caagggccac gaggacatca tcgacgattt tgtgcgcatc tggcccgcgc gggacaactt
      181 ccatttcgag ggggaggtca ccgagggggc ggcccactcg aaatcctcca tcgccgggca
      241 gtcggtggat ttcatcgtgg aggacgggga gctgaagctg ggcggctccc agggcatctt
      301 ttttgccgag tatgccgcgc cccgcgcccg cgagtaccgc gtggcggtgt tcggcgcgtg
      361 aggccctgct gagaaataaa aacgggaagg cgttctcagc cttcccgttt tttgagatgg
      421 gggcgccgtg ccgggcggca cggcgcgggt tcagggcagc ggcgggtaat cggcccgcag
      481 cttttcagcc aaggccacgg cctcccggcg ggtctgttct gtaaagtggg ggtagggccc
      541 gtactgcagc acgttatagg cggcggcgat gttcgccatg acggcggtca tgatggggtc
      601 gaagccctcg gtgtaggcgt acatggcgct gcccgtggag cagttgccgc agccggtggg
      661 gtccaccggc cggccgatat ccacggcggg cacaaaggtg gcggtgttgc cgtacagcgc
      721 gtaggacccg cgggcgccca cccggaagaa gatctgctcc accggcagcc gcagcagctg
      781 cgcgatgatc tcctcctccc gctcgcgggg gatgcccagc agcgcggagg cctcgttcag
      841 gttgcaggaa aaatagtccg ccagcttgca gatctcgcgc acgtcctccc agccgttgtt
      901 gtgctccagc tccagcatga atttgaagcc gtgccgctcc ttgagacggg ccagcttggc
      961 ccagtacact ctgtcggtgt tctgtgccat gtagaccccc ttggtgtggt ccagcgcggc
     1021 ctccagatcc tcggggcggg ttttcaggta gcccaggttc tgcgccccgt agatggattc
     1081 gtgcgcgtat ttgccgtttt ccagatagcg caggacatgc agggtggtgt ggtcggcgcg
     1141 caccgacacc gcgtcggcgg gcacccggtt ggcccgcatc caggggccgt agatctcttc
     1201 aaaatcagcc ccgacgccgg agcagagctt gacgctgtcg gtccacagcc ggatgccggc
     1261 cagggcgaaa aaggccggcc ctcccaggtg catggcgctg cgggagccgt cctcccgctc
     1321 gatgatgtcg ctcatcacat ttccggcggc gatgtaatcc atggatatca gtcctttcaa
     1381 aagcgatacg gataaatttg tttttgtgcc gaggcggccc tgcaacggcc gcgggcggag
     1441 aaaaagagag gtaaactatg cactgtcccc aggacgaccg aaagctgaac cgatattttt
     1501 gcctgctgca ggcgagtatc ttttgcatga tggcgatcca cagcggcttt ttttcgctga
     1561 tgctgtccca acagggcgtc agcaagatgg agctgggcag gatcgcggcg gtgacgggcg
     1621 ccgcctcgct ggtggcgcag cccctgctgg ggctgatcag cgaccgctgg cgggcggagc
     1681 ggctgctcta cgccgtgggg gcggtgctgg ctcctcttgc ctactacaac atccagcaca
     1741 ccacccggct ggggctcatc gctttttggg ccgcgctctt tgtgggcttc agcctggggg
     1801 cgcagatcct cagcacgggc tggatcgcgg ccttgaacgc cgcgggccac aagctggact
     1861 tcggcttttc acggggggtc ggctcgctca gctttgccct ggggtcggtc gtgatcgggc
     1921 aggccatcaa ccgcttcggc atgggctgcc tgccggtcct gctggcggtg ttcgggctga
     1981 ccctggccgc cgccgcgttt cttctgcccc ggccgcagcg cgcccgcccg gcacaagagg
     2041 cggacgcccc gcgcgccgcc ggcttcggcg aggcgatgca tgagctggtc aaaacccggc
     2101 cctatgtgct gctggtgctt tgcagctttc tgggcaatct gccctccggc gctttcacca
     2161 cctacttctc ggtctttttt tcacaggagg gcggcagcgc ctctctgctg gggatcgcgc
     2221 tcttcgtgct ggccatcgtg gaggtgccgg tcatgctgaa ttaccggcgg ctggagcggc
     2281 gcttcggcgt ggagacgctg ctggtggtct cgatgctggg gtacgggctg aagaacctct
     2341 gcctggccct tgctcccagc ctgcccttta tcgtcgcctg tctgctgctg caggccgccg
     2401 gcctggcgct ggcgatcccg gcctcgcaga gcatgatcgc caagatcacg gacaccgcct
     2461 gctgcgccac ggcgcagtgt ctgtggtcct ctctcgggga ggggctgggc atgatcgcct
     2521 ccagcctgtt ctgctcctgg ctggtctctt tcagcagcat ccgcgcggtg ttcgccgtca
     2581 ccagcctgtt cggctttgcg gcggcggccc tcttttgggc gctcg
//
LOCUS       DBMDYA010000424         2622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8544_length_2622_cov_1.737406, whole genome shotgun sequence.
ACCESSION   DBMDYA010000424 DBMDYA010000000
VERSION     DBMDYA010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8544_length_2622_cov_1.737406"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="ACZPFK_10085"
     CDS             complement(<1..2001)
                     /locus_tag="ACZPFK_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1406704.1"
                     /translation="MGKKKILIPNRALRQAICAAVFPEEERGQAYPIESRDLPALRAY
                     QGELRYRGAGGDLSGLAYLENLESLVIESASLRGVRLAVPPRLKKVNLQYCDALEDVS
                     FQNAAALLELSVSGAPELRTLDLSGCFGLERLSVQDCDSLEQLDFSRCRALQSLSCEG
                     LPHGWKMDLSGCPHLKTLTCSAGDAELDLSVLPEPCELNCKGENIKAIKMGAKPCLTS
                     VWLRGLSIEELDLSGCSNLTELHVEDTPLKTIDPAACPELETLVLQRTELETVDLRKN
                     PELLWLDFTGTSLRHLVTGWGDFSGEETGPRGIGANFLPGTPVCRMKPQFSAELQTWW
                     DDPEEYVLRRQGGTDGRTGEWVKGTFLPGVHLNNEPIGTRTCWTPTIRFLSAYEAPVL
                     DGLRVGDPAAKAEALYGSAAMCSEELGLRGWRFEDFYLFLRGTETIEQIGVVKAPHEE
                     ESYSRILERLPELPTDIYKFSEEYPDFPTKWFYGGNLVASSAGLTISNPPLGDYPREV
                     TIYSNYAGEITLEDPDTDYVFCFEDRQFYQEMNYWRQMDAMLENALAVSPSGNGVVIR
                     YRNNSEDKGTFFYALDGGYPLNGYLEMRACSLLGDGYLILSHLNQYGSSFVEIVDLES
                     GEPVPFLTPGERHLLPDQEEGHRESYQLEGADRILIEIDNDLP"
     gene            complement(2443..>2622)
                     /locus_tag="ACZPFK_10090"
     CDS             complement(2443..>2622)
                     /locus_tag="ACZPFK_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406705.1"
                     /translation="RWLASRPQRWEPQEEETLSALARQAVAAATAKVLRPGTQPPQMQ
                     EIASLLPGVQVSMLR"
BASE COUNT          506 a          873 c          669 g          574 t
ORIGIN      
        1 cggcagatcg ttgtcgatct cgatcaaaat gcggtccgct ccctccagct gatagctctc
       61 ccggtggcct tcttcctggt cgggcagcag atgccgttcc cccggcgtga gaaaagggac
      121 gggctctccg ctctcaaggt cgacgatctc aacaaagctg gagccgtact gattcagatg
      181 cgataaaatc aggtagccgt cccccaacag gctgcacgcc ctcatttcaa gatagccgtt
      241 caggggatat cccccatcca aggcgtaaaa gaatgtgccc ttgtcctcac tgttgtttct
      301 atatctgatt accactccat tgccgctggg cgatacagcc agcgcatttt ccagcatcgc
      361 atccatctgg cgccaatagt tcatctcttg ataaaattgc cggtcttcaa aacaaaaaac
      421 gtagtctgtg tccgggtctt ccagcgtgat ttcgcccgca taattgctgt aaatggtaac
      481 ttcgcgcgga tagtccccca gcggagggtt tgaaatggtc agccctgcgc tgcttgcgac
      541 cagattgcct ccgtaaaacc actttgtggg gaaatcggga tattcttctg aaaatttgta
      601 aatatccgtc ggcagctccg gcaagcgctc gagaatacga gaatagctct cctcctcgtg
      661 gggggccttg acgacgccga tctgctcgat cgtctccgtt ccccgcagaa agagataaaa
      721 atcttcaaac cgccatcccc gcagcccgag ctcttcactg cacatcgcgg cggagccgta
      781 gagcgcctcc gctttcgccg cgggatcgcc gacccgcaag ccgtccaata ccggcgcctc
      841 gtaggcgctc aaaaaacgga tggtgggcgt ccagcaggtc cgggtgccga tgggctcgtt
      901 attcaaatgt acccccggca aaaacgtccc tttcacccac tcgcccgtcc tgccgtccgt
      961 cccaccctgc cgtctgagca catactcttc cgggtcgtcc caccacgtct gcaattctgc
     1021 agaaaactgc ggcttcatcc tgcagaccgg cgttcctggt aaaaagttcg ctcctatccc
     1081 ccgcggcccg gtctcctcgc cgctgaaatc tccccatccc gtgaccagat gcctgagcga
     1141 cgtccctgta aaatcgagcc atagaagctc cgggtttttg cggaggtcta ctgtttccag
     1201 ctctgttcgc tgcaaaacca gcgtttccag ctccgggcag gccgccgggt cgatcgtttt
     1261 cagcggcgta tcctccacat gcagctccgt caaatttgaa catccggaaa ggtccagttc
     1321 ctctatggag aggcctctca gccagacgct cgtcaagcac ggttttgcgc ccattttgat
     1381 cgcttttata ttttccccct tacaattcag ctcgcagggc tccggcaaca ccgagagatc
     1441 gagctctgca tcgcctgcgg aacatgtcaa tgttttcaag tgagggcagc cgctcaaatc
     1501 cattttccac ccgtgcggca ggccctcgca ggagaggctc tgcaaggcgc gacatcggga
     1561 aaagtcgagc tgttccagac tgtcgcagtc ctgcaccgac agccgttcca gcccgaagca
     1621 ccccgaaagg tccagcgtcc tcagctccgg cgccccgctg acgctcagct caagcaaggc
     1681 cgccgcattc tgaaaagaga catcttccag cgcatcgcag tattggaggt tcaccttttt
     1741 cagccggggc ggcacggcaa gccgcacccc gcgcaaggag gcgctttcaa tcacaaggct
     1801 ctccagattt tccagatagg ccaggccgga caggtcgccg ccggcccctc tatagcgcag
     1861 ctctccctga taggcgcgca gcgcgggcag gtcccgagat tcgatgggat aggcttgtcc
     1921 ccgctcctct tccggaaaaa ccgccgcaca gatcgcctgg cgcagggcgc ggtttggaat
     1981 cagtatcttc ttttttccca cttctctccc gctcctgccg cccgcctctg cgaacagtcc
     2041 cggcaagaaa caaagaacga tcaaaaaaca aagcgccctc tgcccggcct ttttcaccat
     2101 ttttatcccc gccttaaaaa taagcctttg ttttttcatc cttgtccctt ttctttcccg
     2161 ctgccaaaag ccgtttttta ttcctcctac ggaaccgtcg ctcccgctgc gtgcccagca
     2221 gggttcacgc gggtctttca gaaaggaacg cctccccgtc aaagcggaaa agtccccggt
     2281 cgtctttctt tgtttatccc tctccgggaa agagagcctg cccccgcctc tatggagccg
     2341 cgaggcgacc tcttcgcgga acgcgtgcgt ccgccctttc atcgctgttg gtcccccact
     2401 ttttccgtca aggaatccat cgcctttgca gtggggcctc tctcagcgga gcatcgagac
     2461 ctggaccccc ggcagcaggg aggcgatctc ctgcatctgc gggggctggg tgcccgggcg
     2521 cagcaccttg gcggtggccg cggccaccgc ctgccgcgcc agcgccgaga gggtttcttc
     2581 ctcctgcggt tcccagcgct gtggccggga ggccagccag cg
//
LOCUS       DBMDYA010000425         2616 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8572_length_2616_cov_1.520080, whole genome shotgun sequence.
ACCESSION   DBMDYA010000425 DBMDYA010000000
VERSION     DBMDYA010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8572_length_2616_cov_1.520080"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="ACZPFK_10095"
     CDS             <1..432
                     /locus_tag="ACZPFK_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIR1406706.1"
                     /translation="APARRAAAPEKLEKKSAAPAARQGGKYAAFAAKKTDFRALAPNP
                     DADPQNPFFGKAVVFTGDLALPRAEAAAAVSQLGASVKSSVSRHTDYLVVGMQDAALV
                     GAKGCSTKEQKAAQLNESGKAEIKVLDEPQFMALLAGAKGK"
     gene            complement(656..1246)
                     /locus_tag="ACZPFK_10100"
     CDS             complement(656..1246)
                     /locus_tag="ACZPFK_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406707.1"
                     /translation="MFLPYDLFQKNRTCPPNGQKTPLSRAARVIITARRRLVNKKIRL
                     FQLATFNLLFSFICSVFLRAALMPFFVLKQDKSPARPGFCPAGSGRSGDRRFFHPAYS
                     KPPRVGNNGSCGVSPTKKSASQSAPPSRAAAWRKNKTGPYFNPHRPLRAVTYRDSIAD
                     TAAEVSIHTALAGGDFLEPGSGSDQACFNPHRPCRR"
     gene            1250..1678
                     /gene="rplM"
                     /locus_tag="ACZPFK_10105"
     CDS             1250..1678
                     /gene="rplM"
                     /locus_tag="ACZPFK_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1406708.1"
                     /translation="MSTTLAKPGQIERKWYILDAAGKPLGRVAAKAAVLLRGKDKVIF
                     TPNVDCGDHVIIINCDQAVLTGSKLDKKFYRTHSGWIGGLKEVNYRTLMAEKSDKAMM
                     IAVKGMLPGNSLGAAALKRLRVYKGAEHDNAAQKPEVYAL"
     gene            1697..2095
                     /gene="rpsI"
                     /locus_tag="ACZPFK_10110"
     CDS             1697..2095
                     /gene="rpsI"
                     /locus_tag="ACZPFK_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1406709.1"
                     /translation="MYETKPYFYGTGRRKSSVARVRVYNGTGKITINDRDIDDYFGLE
                     TLKLVVRQPLVLAEIENKFDIVVRVAGGGVSGQAGAIRHGLSRALLLYDENLRPVMKK
                     AGFLTRDPRMKERKKYGLKAARRAPQFSKR"
BASE COUNT          569 a          754 c          807 g          486 t
ORIGIN      
        1 gcgccggccc gccgcgccgc cgcgccggaa aagctggaga agaagagcgc cgcgcccgcc
       61 gcgcggcagg gcggcaagta cgcggccttt gcagcgaaaa agacggattt tcgcgccctg
      121 gcccccaacc cggacgccga cccgcagaac ccgtttttcg gcaaggcggt ggtctttacc
      181 ggcgatctgg cgctcccccg cgcagaggcc gcggccgccg tgtcgcagct tggggcaagc
      241 gtgaagtcct cggtgtcgag gcacaccgat tatctcgtgg tgggcatgca ggacgccgcg
      301 ctggtggggg cgaaaggctg cagcaccaaa gagcaaaagg cggcccagct gaacgagagc
      361 ggcaaagcgg agatcaaggt gttggacgaa ccgcagttca tggccctgct ggccggtgcg
      421 aaaggcaagt gaacacgttt tctcccgcgc gttcgcaccg caaaaagagg agacgcagcg
      481 aagaaagaca gcggccgcgg tgatcatttg agaaagaacc gtgctcacag cgtgcaaagt
      541 tgaagaagat agacaaaata agcgcggaaa cgttgtgtat cttcgcagtc accgcccgca
      601 agggcggtgt ggattgaaac gagcagacca gtgattacgc ctggcgcgat gtgggtcatc
      661 gcctgcaagg gcggtgtggg ttgaaacaag cttgatctga accagatcca ggttccagaa
      721 agtcaccgcc cgcaagggcg gtgtggattg aaacctcggc tgctgtatcc gctatggagt
      781 ctcggtaggt caccgcccgc aaggggcggt gcgggttgaa atagggcccg gttttgtttt
      841 tgcgccacgc cgccgcccgc gagggcggtg cggattgcga cgcgctcttt ttagtggggc
      901 tgacgccgca gcttccgttg ttgcccacgc ggggcggctt ggaatacgcg ggatgaaaga
      961 aacggcggtc accgcttcgt ccgctgcctg cggggcaaaa gcccggccgc gccgggcttt
     1021 tatcttgttt taaaacaaaa aatggcatga gagcggcgcg gagaaaaaca gagcaaataa
     1081 aagaaaataa aagattgaaa gttgccaatt gaaataaacg gatttttttg ttgacaagcc
     1141 tgcggcgggc tgttataata actcgtgcag cccttgaaag gggcgttttc tgcccgtttg
     1201 gcgggcaggt tcgatttttt tgaaagagat catacgggag gaacacgata tgagcacaac
     1261 gttagcaaaa cccggccaga tcgagcgcaa gtggtacatc ctggatgccg ctggcaagcc
     1321 tctgggccgt gttgccgcca aggcagccgt tctgctgcgc ggcaaagaca aggtcatctt
     1381 caccccgaac gtcgattgcg gcgaccatgt catcatcatc aactgtgacc aggccgtcct
     1441 gaccggctcc aagctggaca agaagttcta ccgcacccat tccggctgga tcggcggcct
     1501 gaaagaggtc aactaccgca ccctgatggc tgaaaagtcc gacaaggcca tgatgatcgc
     1561 ggtcaagggc atgctgcccg gcaacagcct gggcgccgcg gcgctcaagc gtctgcgcgt
     1621 ctacaagggc gccgagcatg acaacgccgc acagaagcct gaagtttacg cactgtaaag
     1681 agggggtaac aaagagatgt acgagacaaa accttatttc tacggcaccg gccgccgcaa
     1741 atcttccgtt gcccgcgtcc gtgtgtataa cggcaccggc aagatcacca tcaacgaccg
     1801 cgacatcgac gattacttcg gccttgagac cctgaagctg gtcgtgcgcc agccgctggt
     1861 cctcgccgag atcgaaaaca aattcgacat tgtggtccgc gttgcgggcg gcggcgtttc
     1921 cggccaggcc ggcgccatcc gtcacggcct ttcccgcgcg ctgctgctgt atgacgagaa
     1981 cctgcgcccc gtgatgaaga aagcgggctt cctgacccgc gatccgcgca tgaaagagcg
     2041 caagaagtac ggcctcaaag ccgctcgtcg cgcgccccag ttcagcaagc gctgatcatc
     2101 cattttatct gttttctcca aaaggccccg ctcctgtgag cggggccttt ttatgatacg
     2161 gcggtggggg aggcctgtcc cggccgctgc ctgggtactg cggccgctcc ccattgcacg
     2221 gtcggcattt tcggcttttc atctcttagc cgccctcctg tggcacggcg gatcacacga
     2281 caaaaacagg cgcaggggga cacgatgcca gggcggacag gtgtaaaaga caatagtcga
     2341 gccgctgccc tctattgccg gtgcggcggg acctgcgcgc tctgtgggct gcaaattttt
     2401 gaggacaaaa gacgatagag gcccggcgcc acggcggggt ctgtatgcct ttttgagctg
     2461 tacagcccgc gcgctttctt tcaccggtaa tttgtccggc ccggcacaag aacgttttct
     2521 cacccccttt ccttttcgcg cggtaaaaaa ggagggctcc cgccctgttg gctattgccc
     2581 ccaaaagggt aaaaaaacct ttacagctta cggatt
//
LOCUS       DBMDYA010000426         2605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8604_length_2605_cov_1.253594, whole genome shotgun sequence.
ACCESSION   DBMDYA010000426 DBMDYA010000000
VERSION     DBMDYA010000426.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8604_length_2605_cov_1.253594"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="ACZPFK_10115"
     CDS             complement(<1..562)
                     /locus_tag="ACZPFK_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1406710.1"
                     /translation="MPLTYDLHLHSCLSPCGDNAMTPANIAGFARLNGIRLISLTDHN
                     SAENLPYAQKACDAYGVKLLPGIEMNTAEEIHLLCYLPTVEAALALSREIYALLPDIP
                     CDEAVFGEQIVMDEEDHELQRVEKLLVSACGMGLEEAAARCAALGGVAVPAHVDRESY
                     SVLSVLGIMPEAPVFPAVEVHDPARLP"
     gene            complement(948..1286)
                     /locus_tag="ACZPFK_10120"
     CDS             complement(948..1286)
                     /locus_tag="ACZPFK_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406711.1"
                     /translation="MTVAELCERCGFTPAFLLPEPDKEVCGGFCGDLLSWAMGRAGEG
                     CAWCTVMGSVNAVAVASLADAALIVLCHDAALMEDARARAAEQGINIALTGLPEFEAA
                     VAVAGQLGLR"
     assembly_gap    1394..1403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          435 a          863 c          861 g          436 t
ORIGIN      
        1 gcggcaggcg ggccgggtcg tggacctcca cggccggaaa aaccggggcc tccggcatga
       61 tgcccagcac cgagaggacg gaatagctct cccggtccac atgagccggc accgccaccc
      121 cgcccagggc ggcacagcgg gcggcggcct cctccagtcc catgccgcag gcgctcacca
      181 acagcttttc cacccgctgc agctcgtggt cctcctcatc catcacgatc tgctcgccga
      241 agaccgcctc gtcgcagggg atgtccggca aaagcgcgta aatttcacgc gaaagggcca
      301 gcgccgcctc cacggtgggc aggtagcaga gcagatggat ctcctcggcg gtgttcatct
      361 cgatgccggg cagcagcttt accccgtagg cgtcgcaggc tttctgggca tagggcaggt
      421 tctcggccga gttgtggtcg gtaagcgaga tcaggcggat gccgttgagg cgggcgaagc
      481 ccgcgatgtt ggccggggtc atggcgttgt cgccgcaggg ggacaggcag gagtgcagat
      541 gcaggtcata ggtcagcggc atgggtcggc cccctttcgg cgggagggga aacgccggcc
      601 cggcgtcctc tccccccctc tcttattcat tggacgttaa aggacagaaa agacaaaaac
      661 agcgccccgg cccatggggc gcggagcctg tgcaagcagg gccggtgggc gcggccgtgg
      721 cccatagcct tattctcccg ccgttttttg cgcctggcgg ggcgatgccg gagggctggg
      781 ggacgccgcc cggcgaccct gtggtcaagg ttcgcccaga ccgcgccttt cgcgggggct
      841 tcacggcctc ggggaaacgg acagggaaaa cggagacaga gccacgccct gccgcctgcc
      901 ggcagcctat cgcggctcgt ccggcggagg gaaaagccca aaagggatta ccgcagcccc
      961 aattggccgg cgacggccac cgcggcctca aactccggca ggccggtcag ggcgatgttg
     1021 atgccctgtt cggcggcccg ggcccgggcg tcctccatca acgccgcgtc atgacagagc
     1081 acgatcagcg ccgcgtcggc aagactcgcc accgccaccg cgttgacgct gcccatcacg
     1141 gtgcaccagg cgcagccctc tccggcccgt cccatggccc aggagagcag atcgccgcaa
     1201 aagccgccgc agacctcttt gtccggctcc ggcaggagaa aggcgggggt aaacccgcag
     1261 cgttcacaca gttcggcaac cgtcatggcg gcggcctcct ttcctgcccc cgcgcggggg
     1321 gcgtcccggc gtctgggcgg ccctccccgg ccgggaaagg gggagcgggc cgcgcacgcc
     1381 ttttttattg cttnnnnnnn nnncccaggc taaaaagcag ctggtcggga cggcggaaaa
     1441 cagctttcca ccgccctgcg ggagagggac tcaggaaaac aggacaaaat agaacgatga
     1501 ggagaagata agaggaaagc cgttcgccct gccgcgcggg gaatggagca gaagccgcct
     1561 ccgcccgcgc accgggccgg gggtgttttt gtcttcccgc cggaaacggg gcaaaagcct
     1621 gccgctttgc gctgaaagtt gcgccggaga cggtgggctg gttttgccct cccgcgcggc
     1681 gggcggagca aaagccgctt cccgcagggc tgaggacgct ttcgctctct tgggagaaaa
     1741 catggaacaa aagcccacag ctttgcgctg gaggttgcgc cggagagggc gggatggttt
     1801 cgcctcttct gcgcagaggg tatggcaaag gtgcctgcgc cgcactccga cccttatcag
     1861 ctgcgtttgc ctcttccgca cagtgggcat ggcaaagggc cgcaaaacga tcacacggcc
     1921 gcgcggcgcc tcccggggcg ctgaaccccg actttcgggc ggtgacacct gcagagcaga
     1981 agcgccgggc cgctttctgt gcgggcactt tgtttgcaca ttgctgcaat ccgctcttgc
     2041 ggggcgcgct gttcttgcgg gcgacgcctc atgtaggacg gacttgacgc aatggaaatc
     2101 cccgtttcaa tccgcgccgc tcccgcgagc ggcggcgaga ctgcgatcag ctcaccggcc
     2161 agatcagcag tttccatccg cgccgctctt gcgagcggcg gccaactgat attgtcgcta
     2221 aatcttgtac atttagcgtt tccatccgca ccgctctcgc gagcggcgac tggcttggtg
     2281 gtgctggatt ttgcagagac accgtttttc atccgcgccg ctctggcagg cggcctgtcc
     2341 ggagcatcct ttttgatgtg ctttgaggta cgacatgaag cttccatccg cgccgctctt
     2401 gcgggcaggg acggcggctt tttttccgcg cgcgtcctcg gtgccccgtt gcgtcccgat
     2461 ccctgcgtcc gctgtgcgag cggcaaaaag gggaggcgct cagcccttgc ccgcgcggcc
     2521 gtccttgtcg atctgcagcg ccttgagcac cgcctccatc cgctcgcgca tcagcaccac
     2581 gcagtcgtcc aggtcgccgt cgccc
//
LOCUS       DBMDYA010000427         2603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8609_length_2603_cov_1.884093, whole genome shotgun sequence.
ACCESSION   DBMDYA010000427 DBMDYA010000000
VERSION     DBMDYA010000427.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8609_length_2603_cov_1.884093"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="ACZPFK_10125"
     CDS             complement(<1..656)
                     /locus_tag="ACZPFK_10125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1406712.1"
                     /translation="MKYTAKLMQKPAFCRAENRFVAEGIKLCLDAAASGLAMETVYYT
                     YRAENRWPQLRQLPCEQVRISEPVAEKLSGQRTPQGVFAVCAMPAFDLAAADPAGRYI
                     ALEDVQDPANVGAVLRSAAAFGFSGAILTPGCADPFGQKAMRASMGAVFKLPLFETAD
                     LAGELRRLGGLGAQTFAAALRGAQDLRAVRCAGGGLALAVGNEGNGLSEGVIAACGTV
                     V"
     gene            complement(792..1145)
                     /gene="rplT"
                     /locus_tag="ACZPFK_10130"
     CDS             complement(792..1145)
                     /gene="rplT"
                     /locus_tag="ACZPFK_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1406713.1"
                     /translation="MARIKGAMMTRKRRKKTLKLAKGYWGSKSKHFKMAKQQVMKSGN
                     YAFVGRKAKKRDFRRLWIARINAACRAQGINYSTFMNGLKKAGIDLNRKMLSEMAIND
                     SASFAKLVETAKAAK"
     gene            complement(1230..1427)
                     /gene="rpmI"
                     /locus_tag="ACZPFK_10135"
     CDS             complement(1230..1427)
                     /gene="rpmI"
                     /locus_tag="ACZPFK_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1406714.1"
                     /translation="MPKMKTHTGAKKRFALTKNGKVKRGKAFRSHILTKKTTKRMRGL
                     RKIGYVADANEATIKKLLPYA"
     gene            complement(1429..1992)
                     /gene="infC"
                     /locus_tag="ACZPFK_10140"
     CDS             complement(1429..1992)
                     /gene="infC"
                     /locus_tag="ACZPFK_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1406715.1"
                     /translation="MWRCFTISGKRELELNEQIRDREVRVVGSDSEQLGIMSSRDALR
                     LAAEKNLDLVKIAPTATPPVCRIMDYGKFRFEQQKKEKEARKNQRIVDIKEIRLSLNI
                     DTNDFNTKVNHAKRFLNEGDKVKASIRFRGREMAHTNLGYEVMKRFAQVMSEFATIEK
                     QPKLEGRSMQMYLAPLPKEKVQNKEEK"
     gene            2423..>2603
                     /locus_tag="ACZPFK_10145"
     CDS             2423..>2603
                     /locus_tag="ACZPFK_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HIR1406716.1"
                     /translation="MNWEPWTGCHQISDGCANCYFYGPNAKRYGQNTIQKTDKFDWPI
                     RRNAKGEYCIKGDKIL"
BASE COUNT          468 a          816 c          747 g          572 t
ORIGIN      
        1 accaccgtcc cacaggcggc gatcaccccc tcggacaagc cgttgccctc gttgcccacg
       61 gcgagcgcca gcccgccgcc ggcgcagcgg accgcccgca ggtcctgcgc gccgcgcagg
      121 gcggcggcga aagtctgcgc ccccagcccg cccagccggc gcagctcgcc cgccaggtcg
      181 gccgtctcaa agagcggcag cttgaacacc gcgcccatcg aggcgcgcat ggccttttgc
      241 ccgaagggat cggcgcagcc gggcgtcagg atggcgcccg aaaaaccgaa agccgccgcg
      301 ctgcgcagca ccgcgcccac attggccggg tcctgtacgt cctccagggc gatatagcgc
      361 cccgccgggt cggcggctgc caggtcgaaa gccggcatcg cgcagacggc aaagaccccc
      421 tgtggcgtcc tttgtccgga cagcttttcc gccaccggct cgctgatccg cacctgctcg
      481 cagggcagct gccgcagctg cggccagcga ttttcggccc tataggtata atatactgtt
      541 tccatagcca ggccggaagc ggccgcgtcg aggcacagct ttatgccctc tgcgacaaag
      601 cggttttccg ccctgcagaa ggctggcttt tgcatcagct ttgcggtgta tttcacccgc
      661 tcgttgtccc ggctggtgat ctcgcccgtc gtttttcttc acccggcctt tccggtccgc
      721 cccggttttt caccggggaa taaaacaaag ggacgcccgc gcgcggtcgc gtacgggcgt
      781 tgtttgcaaa cttattttgc agcctttgcc gtctccacca gcttcgcgaa agacgccgaa
      841 tcattgatcg ccatctcgga gagcattttg cggttcaggt cgataccggc ttttttcagg
      901 ccgttcataa aggtggagta attgatgccc tgggcgcggc aggccgcgtt gatgcgggcg
      961 atccacaggc ggcggaaatc gcgctttttc gccttgcggc cgacaaacgc atagttgccg
     1021 ctcttcatga cctgctgttt ggccatcttg aaatgcttgg atttgctgcc ccagtagccc
     1081 ttggccagct tcagcgtttt ctttcttctc ttgcgggtca tcatagcgcc cttgatacga
     1141 gccataattt atttctcctt tatttcacga atagtggcgg gcgggcagcg ccgcgccccg
     1201 gtttggtttt cgactggaag ccgcgccttt tatgcgtagg gcagcagctt tttgatggtc
     1261 gcctcgttgg cgtcggcgac atagccgatc ttgcgcaggc cgcgcatccg tttggtggtc
     1321 ttcttggtca ggatgtggct gcggaacgcc ttaccgcgct tgaccttgcc gtttttcgtg
     1381 agggcgaaac gctttttcgc accggtgtgg gttttcatct tgggcatttt atttttcctc
     1441 cttgttttga accttttcct tggggagcgg agcgaggtac atctgcatgc tgcgtccctc
     1501 cagcttgggc tgtttttcaa tggtggcgaa ctcggacatg acctgggcga agcgtttcat
     1561 cacctcatag cccaggttgg tgtgcgccat ctcgcggccg cggaaccgga tggaggcctt
     1621 caccttgtcg ccctcgttca aaaagcgctt ggcgtggttg accttcgtgt tgaagtcgtt
     1681 ggtgtcgatg ttgagagaca gccggatctc cttgatgtcc acgatccgct ggtttttgcg
     1741 cgcttccttt tccttcttct gctgttcgaa gcggaatttg ccgtaatcca tgatccggca
     1801 gacgggcggc gtcgctgtgg gcgcgatctt gacaaggtcg agattctttt cggccgccaa
     1861 gcgcagcgca tcacgggaag acatgatgcc cagctgctcg ctgtcactgc cgaccacgcg
     1921 tacctctctg tctcgaatct gctcgttcaa ctcaagttct ctcttgccgc taattgtaaa
     1981 acacctccaa ataaactatt gatgcggtcc ttttttgatc agcggagcgc ccggcgggcc
     2041 acccgtcttt tgccgccgcc ctgtgacaaa acggcaaaaa acaaaaaagc ggaggcgcaa
     2101 cgcccccact ctcacatccg tatcgacagg cccaaagcct gaaaacgtct gttgacccgg
     2161 cacccttggg gcacgcaagg tgagtgcggc aatgcgcagc ttctttactg actaaattag
     2221 tttagcacag gggccccgcc ctgtcaagcc ccaaaacaca cctgcggcgg cccgcccgcg
     2281 ggcaggccca gacagtcttt cagccccgct tgaggcgggc gggggcgata tgatatcata
     2341 gacccaaggc cctctcccgc gccagcggga gggacgggcc ctcaaaattt ggtctcaaac
     2401 ggaaccgacg gaggaaaagc cgatgaactg ggaaccatgg accggctgcc atcagatcag
     2461 cgacggctgc gcaaattgct acttttatgg gccgaacgca aagcgctacg gccaaaacac
     2521 catacaaaag accgacaagt tcgactggcc catccgccgg aatgcaaagg gggaatactg
     2581 catcaagggg gacaagatcc tcg
//
LOCUS       DBMDYA010000428         2581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8687_length_2581_cov_1.387097, whole genome shotgun sequence.
ACCESSION   DBMDYA010000428 DBMDYA010000000
VERSION     DBMDYA010000428.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_2581_cov_1.387097"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(258..542)
                     /locus_tag="ACZPFK_10150"
     CDS             complement(258..542)
                     /locus_tag="ACZPFK_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR1406717.1"
                     /translation="MYQDKTLVCKDCGNEFVFTAGEQEFYASRGFENEPQRCKACRDA
                     RKNGGRGQREMFTAVCASCGKEARVPFKPREDRPVYCSECFARMKEEENG"
     gene            complement(684..1274)
                     /locus_tag="ACZPFK_10155"
     CDS             complement(684..1274)
                     /locus_tag="ACZPFK_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1406718.1"
                     /translation="MDCRKHVAKERLSPMSEQTEAVRVRSARGGDEAALAALIAKFMP
                     VIRRFARAAVCPGLDFDDAVQEGYIGLFHAVKSFEEEGGQASFSTYATVCIRNAVVAA
                     RRAAGRKKHQTLSGALPLDEQVTAPGPEELAILKEQVHEAVKSIDTELSSFEKKALLL
                     SLRGQSYTEIATELSKTPKAVDNALVRVRRKLKRTP"
     gene            complement(1457..>2581)
                     /locus_tag="ACZPFK_10160"
     CDS             complement(1457..>2581)
                     /locus_tag="ACZPFK_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406719.1"
                     /translation="KKDLDTARRVMSISMRLVLIISVLFTAVSLAFPRQLMGLFSHEE
                     AVVDAASSYLFVLAFSYIFFSVSSNYIMSLRAVEQVKVSAVIYGISFFVNVFFNYCFI
                     FGKFGFPRMEVAGAAVGTIFARVSEFIMVLIYMAFFEKRVQFKPRHLLSFDRGLLPDY
                     IRHALPVVGNEVLWGLGAAATNLVIGRIGSSFVAANSIANVMNQLCGVFIFGIANAAA
                     VLTGKAIGQGKSGHAQQTAQTLWAMSFGVGVFSCLVLLALRGPVMMIYNVTPEAHAAA
                     TSILTVLALLQISFAPDITNIVGILRGGGDTRTAFLFDCGGLWLIGVPLGILTGLVLR
                     LPAPIVFLCMKMDSPVKLVLGSVRMFSGKWIRNVTRAEEG"
BASE COUNT          547 a          807 c          716 g          511 t
ORIGIN      
        1 gccgggcaac cttttttcga aagcgcgtct ttctccgggg cgtctcccac gcccgcaggg
       61 ccgggaggga gaaagagggc tcgggcccct ccttgtcccc aaggccttac ggctttcttc
      121 atccatttca aagggaggct ttttcggctg cgggccgctg ccaaccgccg ttcgcagcgc
      181 cgctctcgcc cccacaaaaa aacgcgcctt tctcacgaaa ggcgcgtttt tgcagtcact
      241 gttccggggg acggctctca gccgttctcc tcctctttca tgcgcgcgaa gcactcgctg
      301 caatagacgg gacgatcctc gcggggctta aagggcacgc gggcttcttt gccgcagctg
      361 gcgcaaacgg cggtgaacat ctcgcgctgg ccacggccgc cgttcttgcg ggcgtcgcgg
      421 caggccttgc agcgctgcgg ctcgttttcg aagccacggc tggcatagaa ttcctgttca
      481 ccggcggtga aaacgaactc gttgccgcaa tctttgcata ccaatgtttt gtcctgatac
      541 atgcttagtt ctcctctaac atagtttttt gcccgcggaa ggattcaaac cgacaccttt
      601 ggtaaggacc aacgctctgt tttaagctac atggggcgta tatcaaaagg ccctgaggcc
      661 ctttttgaac cgacagcacc gaatcacggg gttctcttga gttttctgcg cactctcaca
      721 agggcattgt ccactgcctt cggtgttttt gacagttcag tcgcgatctc ggtgtagctt
      781 tgtccccgca gggagaggag gagcgccttt ttttcaaagc tggacagctc tgtatcgatg
      841 ctcttcaccg cttcgtgaac ctgttctttt aaaatagcga gctcttcggg cccgggtgca
      901 gtcacctgtt catccagcgg cagcgcgccg ctgagcgttt gatgcttttt gcggccggcc
      961 gcacgccgtg ccgccacaac ggcatttcgg atgcagaccg tcgcataggt ggaaaaggag
     1021 gcctgtccac cctcttcctc aaagctcttc accgcgtgaa aaaggccgat atatccctct
     1081 tgcaccgcgt cgtcaaaatc aagccccgga cacaccgccg cacgcgcgaa ccggcgaatc
     1141 acgggcatga actttgcaat cagcgcggcc agggccgcct cgtcgccgcc gcgcgcactg
     1201 cgcaccctga cggcttcagt ttgctcggac ataggggaaa gcctttcctt tgcaacatgt
     1261 ttcctgcaat ccattatagt atagggtcaa acccgcaaat tgtcaatagt ttccgcaaaa
     1321 acacggctct ctatcacaaa atttttgttc aatctgcacg atattgcatc gtcaagagag
     1381 ggcaggatgc ctgcccgtca agcatctttc gcgcccatga cccgcctttt taaaagcgaa
     1441 cgcggcaaac gtgcggtcag ccctcttccg cccgcgtgac attgcggatc catttgcctg
     1501 aaaacatgcg caccgacccc agcaccagct tgaccggcga gtccatcttc atgcacaaaa
     1561 acacgatggg cgcgggcagt cgcagcacca ggccggtgag gatgcccagc ggcaccccga
     1621 tcagccaaag gccgccgcag tcgaacaaaa aggcggtgcg cgtgtccccg cccccgcgca
     1681 ggatgcccac gatgttggtg atatccggcg caaaggagat ctgcagcaaa gccagcaccg
     1741 tgaggatcga ggtggcggcg gcatgggcct ccggcgtgac gttgtagatc atcatcaccg
     1801 ggccgcgcag ggccagcagc accaggcagc tgaacacccc caccccaaag gacatggccc
     1861 acagggtctg ggcggtctgc tgcgcgtggc cgcttttgcc ctgcccgata gccttgcccg
     1921 tgagcacggc ggccgcgttg gcgatgccga agataaagac gccgcagagc tggttcatga
     1981 cgttggcgat gctgttggcc gccacaaagc tggagccgat gcgcccgatc accagattgg
     2041 tcgccgcggc ccccaggccc cacagcacct cgttgcccac caccggcagg gcgtgacgaa
     2101 tgtagtcggg cagcaaaccg cggtcaaagg acagcagatg ccgcggcttg aactgaaccc
     2161 gcttttcaaa aaacgccatg tagatcagca ccatgataaa ctcggacacg cgggcgaaga
     2221 tcgtgcccac ggccgctcct gcgacctcca tgcgcggaaa gccgaatttg ccgaaaatga
     2281 agcagtagtt gaagaataca ttgacgaaaa acgagatgcc gtagatgacg gcggacactt
     2341 ttacctgctc taccgcgcgc aggctcatga tatagttgct ggaaactgag aaaaagatat
     2401 agctgaatgc cagcacgaac agatagctgc tggcggcgtc caccaccgcc tcctcatggg
     2461 agaacaggcc catgagctgg cgtggaaagg ccagcgacac cgcggtaaaa agaaccgaga
     2521 tgatgagcac cagccgcatc gagatgctca tcacccgccg cgccgtatcc aggtcctttt
     2581 t
//
LOCUS       DBMDYA010000429         2579 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8693_length_2579_cov_1.552462, whole genome shotgun sequence.
ACCESSION   DBMDYA010000429 DBMDYA010000000
VERSION     DBMDYA010000429.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_2579_cov_1.552462"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="ACZPFK_10165"
     CDS             <1..562
                     /locus_tag="ACZPFK_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1406720.1"
                     /translation="EPTTPAQANDQDFTKNQQLVLDNKALFMPNGTWIVGEMAKAPRA
                     DGFKWGMTALPAVEAGGDGYSYTWFEQAWIPAGAANPDAAKQFVAFLYSDKACEIFAK
                     AGAIQPVLNIADKLEGDNQMFYAIYDNGAKAAMGNFAATDAIEGLDIRTVWFDPVNSL
                     VAGTMTEEQWIDGIKSASDQLRGALK"
     gene            713..1600
                     /locus_tag="ACZPFK_10170"
     CDS             713..1600
                     /locus_tag="ACZPFK_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1406721.1"
                     /translation="MEKGKGRKRFLFFCVAPAVLLFTIFMVIPTLNVFRMSLFRRSAY
                     SPNEEFVGLENFVKLFNDANFIRSMQNTILLIVVVTIVTFGFALVFAGILTREKIRGQ
                     NFFRVIFYIPNILSVVVISAIFSAIYDTNNGILNSLLSLFSPEKVAILWKGEGLVMTS
                     LIIAMVWQAIGYYMVMYMASMSSVPESVYESANLDGAGRVTQFFRITLPLIWTNIRTT
                     LTFFIISTINMAFLFVKAMTSGGPNGASEVALSYMYKQAYTNSSYGYGMAIGALVFLF
                     SFALSAVVNKVTERDALEF"
     gene            1614..2501
                     /locus_tag="ACZPFK_10175"
     CDS             1614..2501
                     /locus_tag="ACZPFK_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1406722.1"
                     /translation="MEQVKKASGGDRLYKVFIYVALITLAVLILVPVAWVFLASVKEN
                     SEFYGNPWALPAGFHFQNFVEAWTEARMGDYMLSSIFVTALALVILLVVALPAAYALS
                     RFNFRGRHFFNTCFMAGLFINVNYIVVPIFLMLVDGDKFLRGVFGKGFLLNNIVVLAL
                     VYASTALPFTIYLLSGYFSTLPHAYEEAAYIDGAGYGRTMVKIIFPMAKPSIITIILF
                     NFLSFWNEYIIAMTLLTDPNGTRTLPVGLMNLTQAQQSAAQYGRLYAGLVLVMLPTLL
                     YICVQKKLTQGMTLGGLKG"
BASE COUNT          484 a          824 c          727 g          544 t
ORIGIN      
        1 cgagcccacc accccggcgc aggccaacga ccaggacttc accaaaaacc agcagctggt
       61 gctggataac aaagccctgt tcatgcccaa cggcacctgg atcgtgggcg agatggcgaa
      121 agccccccgc gccgacggct tcaagtgggg catgaccgcc ctgcccgccg tggaggccgg
      181 tggcgacggc tacagctaca cctggttcga gcaggcctgg atccccgccg gcgccgccaa
      241 ccccgacgcc gccaagcagt tcgtcgcttt cctgtacagc gacaaggcct gcgagatctt
      301 tgccaaggcc ggcgccatcc agcccgtgct caacatcgcc gacaagctgg agggcgacaa
      361 ccagatgttc tacgccatct atgacaacgg cgcgaaagcc gccatgggca acttcgccgc
      421 caccgacgcc atcgagggcc tggacatccg caccgtctgg tttgaccccg tcaacagcct
      481 ggtggccggc acgatgaccg aagagcagtg gatcgacggc atcaagtccg ccagcgacca
      541 gctgcgcggc gcgctgaaat aagcaccgca ggcaccgctg aagcaacaac acagcaggaa
      601 aagcggcggt gagcagtccc ctgctcaccg ccgctttttt caggggggag ggcaaggcgc
      661 gggaaaagcg tcccgcgccc cctgcgcccc tgttcagaaa ggagatgagc tcgtggaaaa
      721 aggcaagggc cgcaagcggt ttttgttttt ctgcgtggcg ccggccgttc ttttgttcac
      781 catcttcatg gtgatcccaa ccctcaacgt cttccgcatg tccctgttcc gccgcagcgc
      841 ctattccccc aatgaggagt ttgtggggct ggagaacttt gtcaagctct tcaacgacgc
      901 caacttcatc cgctcgatgc agaacacgat tttgctgatc gtggtggtta ccatcgtgac
      961 ctttgggttt gcgctggtct ttgcgggcat tttgacccgt gaaaagatcc gcgggcagaa
     1021 tttcttccgg gtgatcttct acatccccaa catcctgtcg gtggtggtca tctcggccat
     1081 cttctcggcc atctacgaca ccaacaacgg catcctcaac agcctgctct ccctcttttc
     1141 acctgaaaag gtcgccatcc tgtggaaagg cgagggcctg gtgatgacca gcctgatcat
     1201 cgccatggtc tggcaggcca tcggctatta catggtcatg tacatggcct cgatgtccag
     1261 cgtgcccgag agcgtttatg agagcgccaa cctggacggc gcgggccggg tgacccagtt
     1321 tttccgcatc acgctgccgc tgatctggac caacatccgc accaccctga ccttttttat
     1381 catctccacc atcaacatgg cgttcctgtt tgtcaaggcc atgaccagcg gcggccccaa
     1441 cggcgcctct gaggtggcgc tgagctatat gtacaagcag gcctatacca actcttccta
     1501 cggctacggc atggccatcg gcgcgctggt gttcctgttt tcctttgcgc tgtccgccgt
     1561 ggtgaacaag gtcaccgagc gcgacgcgct ggaattctaa gggggtattt ttcatggagc
     1621 aagtcaaaaa ggcctccggc ggcgaccggc tgtacaaggt tttcatctat gtggcgctca
     1681 tcaccctggc ggtgctgatc ctggtgccgg tggcctgggt gtttctggcc tccgtcaagg
     1741 agaacagcga attttacggc aacccctggg ccctgcccgc aggctttcac tttcaaaact
     1801 tcgtcgaggc ctggaccgag gcgcgcatgg gcgactacat gctcagttcc attttcgtca
     1861 cggcgttggc gctggtgatc ctgctggtgg tggcgctgcc cgccgcctat gcgctgtccc
     1921 gcttcaactt ccgcggccgg catttcttta acacctgttt catggcgggg ttgttcatca
     1981 acgtcaacta catcgtggtg cccatctttt tgatgctggt ggacggcgac aaattcctgc
     2041 gcggggtgtt cggcaaaggg tttttgctca acaacatcgt ggtgctggcc ctggtctacg
     2101 cctcgacggc gctgcccttt accatctatc tgctgtcggg ctatttttcc accctgcccc
     2161 acgcctatga ggaggcggcc tacatcgacg gcgcgggcta cggccgcacc atggtcaaga
     2221 tcatcttccc gatggcgaag cccagcatca tcaccatcat cctgttcaac tttctctcgt
     2281 tctggaacga gtacatcatc gccatgaccc tgctcaccga cccgaacggc acccgcaccc
     2341 tgccggtggg cctgatgaac ctgacccagg cgcagcaatc cgccgcccag tacggccgcc
     2401 tgtatgccgg cctggtgctg gtgatgctgc ccacgctgct gtatatctgc gtgcagaaaa
     2461 agctgaccca gggcatgacc ctgggcgggc tgaaaggatg aggtgagacg atgcaatttt
     2521 ggaaagagca tgaaaaactg cgcctggcgc tcatcgccgc ctttttcctg gtggggctg
//
LOCUS       DBMDYA010000430         2574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8718_length_2574_cov_1.469875, whole genome shotgun sequence.
ACCESSION   DBMDYA010000430 DBMDYA010000000
VERSION     DBMDYA010000430.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8718_length_2574_cov_1.469875"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="ACZPFK_10180"
     CDS             complement(<1..874)
                     /locus_tag="ACZPFK_10180"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1406723.1"
                     /translation="MKELELKYGCNPNQKPAKLLVREGELPLEVLNGKPGYINFMDAL
                     NSWQLVRELKKATGLPAAASFKHVSPAGAALGLPLSDTLKKIYFVEGMELSPLACAYA
                     RARGADRMSSFGDWIALSDECDAATASVIKREVSDGVIAPGYSAEALQILKSKKKGNY
                     NVVRIDPDYQPAAIEHKDVFGITFEQGRNSLQVTEAMLENVVTANKALTAEQKRDLML
                     ALITLKYTQSNSVCYAKDGQAIGVGAGQQSRIHCTRLAGNKADIWHLRQHPKVLGLQF
                     KEGIGRADRDNAIDV"
     gene            complement(992..1762)
                     /locus_tag="ACZPFK_10185"
     CDS             complement(992..1762)
                     /locus_tag="ACZPFK_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019443.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1406724.1"
                     /translation="MATQDIAELLRQNSYPGRGIILGRSEDGRNAVAAYFIMGRSANS
                     RNRVFTERDGGIITEAADESKMVDPTLIIYAPVRVLGDQITIVTNGDQTDTIYDCMEN
                     GESFSKALRKRSFEPDGPNYTPRVSSYLKVTGGRMRYRMGIIKSDDGDPDSVERFFYE
                     YPKPLPGEGRFLHTYDGDGDPIPSFTGEPRRIAIRGDIDAFTRTLWESLNEANKVSLF
                     VRYIPLGDGRPETRIVNKYKKVDAAPVGKEPFHPGMIK"
     gene            complement(1862..2449)
                     /gene="purN"
                     /locus_tag="ACZPFK_10190"
                     /pseudo
     CDS             complement(1862..2449)
                     /gene="purN"
                     /locus_tag="ACZPFK_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            complement(2443..2565)
                     /locus_tag="ACZPFK_10195"
     CDS             complement(2443..2565)
                     /locus_tag="ACZPFK_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014788.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406725.1"
                     /translation="MCLVVAAADADRALAVLRENGEDAYLLGELAAGEGGVELW"
BASE COUNT          371 a          865 c          850 g          488 t
ORIGIN      
        1 agacgtcgat ggcgttgtcc cggtcggcgc ggccgatgcc ctctttgaat tgcaggccga
       61 gcaccttcgg gtgctggcgc aggtgccaga tgtccgcctt gttgcccgcc agccgggtgc
      121 agtggatgcg gctctgctgg cccgcgccca cgccgatggc ctgcccgtcc tttgcatagc
      181 agaccgagtt ggactgggtg tatttcaggg tgatgagggc cagcatcagg tcccgcttct
      241 gctccgcggt caaggccttg ttggcggtga ccacattctc cagcatggcc tcggtgacct
      301 gcaggctgtt gcgcccctgc tcaaaggtga tgccgaacac gtccttgtgc tcgatggcgg
      361 cgggctggta atccgggtcg atgcgcacga cgttgtaatt gccctttttc ttgcttttca
      421 aaatctgcag cgcctcggcg gaatagcccg gcgcgatgac gccgtccgag acctcccgct
      481 tgatgacgct cgccgtggcc gcgtcgcact cgtccgagag ggcgatccag tcgccgaagc
      541 tggacatgcg atccgcgccg cgggcgcggg cataggcgca ggccaggggc gagagctcca
      601 tgccctcgac gaaatagatc tttttcaggg tgtcggacag gggcaggccc agcgcggcgc
      661 ccgccgggct gacatgcttg aagctcgccg cagcgggcag gccggtggct tttttcagct
      721 cccgcaccag ctgccaggag ttgagcgcgt ccataaagtt gatgtatccg ggcttgccgt
      781 tcagcacttc cagcggcagc tctccctcgc gcaccagcag ctttgcgggc ttttgattgg
      841 ggttgcagcc gtatttgagt tccagctctt tcattgcaaa acgcgtcctt tcggtttgtt
      901 cctgttttct gcgcccccgg ggcggccgcg gccttgcctg gccgccgcac gccggcggcg
      961 gaaagccccg tgtgcgggga gaaacggggt cttacttgat catgccgggg tggaaaggct
     1021 ctttgcccac cggggcggcg tccacctttt tatacttgtt gacgatgcgg gtctcggggc
     1081 gcccgtcgcc cagcgggata tagcgcacaa aaaggctcac cttgttggcc tcgttgaggc
     1141 tttcccacag ggtgcgggta aaggcgtcga tgtccccccg gatggcgatg cggcgcggct
     1201 cgccggtaaa gctggggatg gggtccccgt cgccgtcata ggtgtgcaga aagcgcccct
     1261 ccccgggcag cggcttcggg tattcgtaaa agaaccgctc caccgaatcc gggtcgccgt
     1321 catcgctttt gatgatgccc atgcggtagc gcatgcggcc gccggtgact ttcagataac
     1381 tgctcacccg cggggtgtaa ttggggccgt ccggctcgaa cgagcgcttg cgcagcgctt
     1441 tcgaaaagct ctcgccgttt tccatgcagt cgtagatggt gtcggtctga tcgccgttgg
     1501 taacaatggt gatctggtcg cccagcaccc gcaccggcgc gtagatgatc agggtcgggt
     1561 ctaccatctt gctctcgtcg gcggcctcgg tgatgatgcc cccgtcccgc tcggtaaaga
     1621 cgcggttgcg gctgttggcg ctgcggccca tgatgaaata agcggcgacg gcgttgcggc
     1681 cgtcctcgct gcggccgagg atgatgcccc ggccgggata gctgttttga cgcagcagct
     1741 ctgcgatgtc ctgtgtggcc atagctctcg gctccttttc tggttcgatt gatttttgta
     1801 cccgcccctc tgcggcgggg cggcaggggc aggtgtggga ggcaagcggc tccacgccct
     1861 cctcgcgggc gcagatcagc tctaccgcgc ggggcagcag ctgccattcc gcctgctcca
     1921 tcacccggcg ctgcagggtc tcgggcgtgt cgccgggcag gacctccacc gccttttgca
     1981 gcaggatctc gccgccgtcc ggtacctcgt tgacaaagtg caccgtggcc ccggtcaccc
     2041 gcaccccctt ggccagcgcc gcctcgtgga cccgcaggcc gtacatgccc gccccgcaaa
     2101 agctggggat caggctgggg tggacgttca ggatgcggcc cggataggcc gccaccaccg
     2161 ccgggcccag gatggagagg aagcccgcca gcaccacgac ccggatgtcg tgcgcccgca
     2221 gcacctcgcg cagcgcggcg tcaaaggacg cgccgtcggg gtattctttg cggcagacca
     2281 cctgcgcggg cacatcggcc tttgccgccc gccccagcgc ggcgacgccg gggcggctgg
     2341 agaccaccag cgcgatctgc gcgtggggca gcgcccccgc ggccgcggcg tccaggatcg
     2401 cctgcaaatt ggtgccgccc ccggagacca gcaccgccac ctttaccaca gctcgacccc
     2461 gccttcccca gccgccagct cgcccagcag gtaggcgtcc tcgccgttct cgcgcagcac
     2521 ggcgagcgcc cggtccgcgt cggcggccgc caccaccagg cacatgccca caga
//
LOCUS       DBMDYA010000431         2567 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8751_length_2567_cov_1.461882, whole genome shotgun sequence.
ACCESSION   DBMDYA010000431 DBMDYA010000000
VERSION     DBMDYA010000431.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8751_length_2567_cov_1.461882"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..771
                     /locus_tag="ACZPFK_10200"
     CDS             145..771
                     /locus_tag="ACZPFK_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR1406726.1"
                     /translation="MYPTCASCTVQACAKGTTEGMPKNCPMRDPGFFEQTFPEYQKAE
                     NHDFYVTASEIEGLGYGEWCRLKETLELFKAMRYQRIGIAFCQGLSREARIISQIFVR
                     HGFEVASVICKTGGIEKEQVGIARERKVNPEAAEMMCNPISQAKLLNLHGTQFNVLVG
                     LCVGHDSMFYKYSDALVTTLIAKDRVLAHNPAGAVYCSEGYYKKKLGL"
     gene            complement(1153..1992)
                     /locus_tag="ACZPFK_10205"
     CDS             complement(1153..1992)
                     /locus_tag="ACZPFK_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406727.1"
                     /translation="MKIGYTGWTWDTDEYRDWAPFNEFHKENFEQFLREVSNLGYETV
                     EDFSFIADYYEDDVQGFKDVVAKYGLKFENLYFYFTDDEAADCENAKKYIAFAKEVGA
                     HHMNMQGVMWKDTPFTRPTDKEKILSYARRSNIIGKLCAEAGIKACMHPHANTAVFTE
                     EQIDLFCANTDPQYVWLCLDTAHVTLAGMDAPKAAKKYGKRVGYMHLKDLDPNPEAHP
                     EWPMRRFLPLGYGCVDFRGVVRGLKEEGYDGILCVELDYQPVCNYKSAMDSRNYLRNV
                     LGF"
     gene            complement(2491..2566)
                     /locus_tag="ACZPFK_10210"
     tRNA            complement(2491..2566)
                     /locus_tag="ACZPFK_10210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2531..2533),aa:Asn,seq:gtt)
BASE COUNT          574 a          665 c          769 g          559 t
ORIGIN      
        1 ttgccggaaa atgtgctttc agcataccac ggcgcggaca aaaagtcgaa ttgatctgcc
       61 tggcgttgac aaaggccgcc cacgggccta agatggtagg gagaaaaaat gcgggcgttt
      121 ttccgcccgc agaaagaggt aaccatgtat ccgacctgtg ccagttgtac cgtccaggcc
      181 tgtgccaagg gcacgaccga ggggatgccc aagaactgtc ccatgcggga ccccggcttt
      241 tttgagcaga cctttcccga atatcaaaaa gcggaaaacc acgattttta tgtcacggcc
      301 tctgagatcg aggggctggg ctatggggag tggtgccgcc tcaaagagac gctggagctg
      361 tttaaggcca tgaggtacca gcgcatcggc attgcctttt gccagggcct gtcgcgggag
      421 gcgcgcatca tcagccagat ttttgtgcgg cacgggtttg aggtggcatc ggtcatctgc
      481 aagacgggcg gcattgaaaa agagcaggtg ggcattgccc gggaacgcaa ggtgaacccg
      541 gaggcggccg aaatgatgtg taaccccatt tcccaggcaa agctgctcaa cctgcacggc
      601 acccagttca acgtgctggt ggggctctgc gtggggcacg attccatgtt ttataaatac
      661 tcggatgcgc tggtgaccac cctcatcgcc aaggaccggg tgctggccca caaccccgcc
      721 ggcgcggtct actgctcaga ggggtattac aagaaaaaac tggggctgtg agaacgatgc
      781 gggcgggcgg ctgaggacaa agcacaccgc tcttttggcg gcgcgggttt tccggtctgc
      841 ccggctgaga gaggcgggga gaaaagcggg cgaaaagccg ccccgcgccc tgcgggaagc
      901 cgtttttgct gcattgcttt aatgcccgga cagggagggg cggtggtcgg gcccggggcc
      961 gcggtagagc cgctttttac atccgccctt tgcctctgtt tggctgttct gcgccggcgg
     1021 cgcccgcaag cggaaaggca tgggccggcc gccctataag gtcggttttg cagtataaaa
     1081 agggacggtc ttttgccgct acgcggcaaa agaccgtccc ttttttggga tacaggagag
     1141 agtgtgaaaa gcttaaaagc ccaggacgtt gcgcaggtag ttgcggctgt ccatggcgga
     1201 tttgtagttg cagaccggct gatagtcgag ctcgacgcag aggatgccgt cataaccctc
     1261 ttctttcagg ccgcgcacca caccccggaa atccacacag ccgtagccca gcggcagaaa
     1321 acggcgcatc ggccattcgg gatgggcctc cgggttggga tccaggtctt tcagatgcat
     1381 atagcccacc cgtttgccgt actttttcgc ggccttgggg gcgtccatgc ccgccagggt
     1441 gacatgggcg gtgtccaggc agagccatac atactgggga tcagtgttgg cgcagaagag
     1501 gtcgatctgc tcctcggtga acacggcggt gttggcgtgg ggatgcatgc aggccttaat
     1561 gcccgcctct gcgcacagct tgccgatgat attgctgcgg cgcgcatagc tgaggatttt
     1621 ttccttgtcg gtggggcggg taaagggggt gtctttccac atcacaccct gcatgttcat
     1681 gtggtgggcg cccacctctt tggcaaaggc gatatatttt ttcgcattct cgcagtccgc
     1741 cgcctcgtcg tcggtgaaat aaaagtagag attctcaaat ttcaggccat atttggcgac
     1801 cacatccttg aagccctgta cgtcgtcctc ataatagtcg gcaataaagc tgaaatcctc
     1861 cacggtctcg tagcccaggt tcgagacctc gcgcaaaaat tgctcaaagt tttctttgtg
     1921 aaactcattg aacggggccc agtcacgata ttcgtcggtg tcccaggtcc aaccggtgta
     1981 cccaattttc ataacagatc atccctccgt ttttttggtt gctgcatggt ttacagggcg
     2041 ctcgcccggc attttccttg ggctgccggc gccgcgggcg cgatcgtctt ttgagaaaca
     2101 aattcgtttc tgtaccgagt atatttgagt aactttgttg cgtaaactgt tgcaatcgca
     2161 aaaaaacgga ggaggttttt gacggatttg tacaaagagg gggcgagaca ctgcggaatg
     2221 caacggaaat atccgtgaat tccctaccgt cgctcacttt gctctaatgc ggggcccgga
     2281 gaaaatgccg cggccgttgt cataaagaag aggaaatgac ttttgtacct gaccatacaa
     2341 ctaagtattt caggcgtcaa agcgacaaaa agatgaaagg gacaaaagaa tgaggaaaga
     2401 atgtcagaga gggacagcaa aaaatgtggt atttgcaaaa gaaaaaccgc accaaacagc
     2461 cgaaaaacgg cggtttggtg cggttttttc tggctccccc tgttgggctc gaaccaacga
     2521 ccctgcggtt aacagccgca tgctctaccg actgagctaa ggaggaa
//
LOCUS       DBMDYA010000432         2565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8755_length_2565_cov_1.512987, whole genome shotgun sequence.
ACCESSION   DBMDYA010000432 DBMDYA010000000
VERSION     DBMDYA010000432.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8755_length_2565_cov_1.512987"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            621..1910
                     /locus_tag="ACZPFK_10215"
     CDS             621..1910
                     /locus_tag="ACZPFK_10215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1406728.1"
                     /translation="MKRKKRKITPSLLALSVLPLLAFGLIVSLITSRLIYTSLRDEVE
                     YSLNVLIHNAYAIGQLLYPGEYSERDGELYKGGQPLAGRFEIVDEMQASAGVDATLFY
                     GDERYLTTIRTPDGARASGTPAQPEVVQRVLRDGEDFFSDRVLVNGVPYFGYYMPLTK
                     ADGTAVGMLFVGRPRAQVMKSIDRNILQVCLSEAGILAIAVGLAIYYSRKMVYSLNKT
                     EKFLGEIASGDLTAHIDPYLLERQDEVGEMGRFAVVLQRSIEDLVGKDPLTGLYNRRS
                     CDVVLQSLAERAQRGGRPFTVVMGDIDYFKKVNDDYGHQAGDAVLKAVAETLSAHMER
                     RGFVFRWGGEEFLLLYEEMDEKAALLRLKRLQGELARAKVEWQGTPIRVTMTFGVADS
                     THSPQPEALIRLADENLYLGKQEGRDRVVSAREEREL"
     gene            1913..2392
                     /locus_tag="ACZPFK_10220"
     CDS             1913..2392
                     /locus_tag="ACZPFK_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406729.1"
                     /translation="MGHWGFSWTGLLFLALLMAPNLLWTKNKPKGYSAAGEDRRLLAL
                     ERIGEAGTVCTALVFRDTNPGPWSAWSLWLAAAALLMLLYECWWARYFKSGKQLSDFY
                     SSFLGVPLAGATLPVAAFGLLGVYGRLLPLLFFTLVLGVGHIGIHLQHYRALARGRG"
BASE COUNT          453 a          726 c          928 g          458 t
ORIGIN      
        1 catcaacctc tcccgcaagg acgcgctggc cgacatggca aagcgccagg ccgagcgcgc
       61 cccggccgga gagtaagccg ggcgcaaaaa gtgcccgcag cggccgtttt ccggccgccc
      121 gggccatctt ttggccgcgg tgtcgggctg agttgttttt atcgccaagc aatcaaaaag
      181 agggagaaag cggtcaccgc tttctccctc tttttggata gcgattttat agcgagtgac
      241 tggaaagaga agataagaga cagatatttc ctgaaacaag aagtgttgct cttgtttttg
      301 tgtcggaaac tgttatactg gaaacattgg gcggccctct gccctgtccg tcgcccgcaa
      361 gggagctttt gcggggaggg cgggaggagg catgtccctg tgccgccggc cgggagagcc
      421 cgggccgatg cggacgatgg aggaggccgg ggcatccccg aggcgctgtc tgagccgagg
      481 gggcgcccgg ggcagccggg ccgttttggg cttcgcccgg gggaggggca gagggaagac
      541 gcgtccgcct cccaactttg ggaaaaggaa aaaaatacgg gcccgcggcg tcggcggcgg
      601 agaccggagc gaggtttgtc atgaagcgga aaaagcgaaa aattaccccc agcctgttgg
      661 cgctctccgt cctgcccctg ctcgctttcg ggttgatcgt ctcgctcatc acctcgcgcc
      721 tgatctatac cagcctgcgg gacgaggtgg aatacagctt gaacgtgctg atccacaacg
      781 cctacgccat tggccagctg ctgtatcccg gcgaatacag cgagcgggac ggcgagctgt
      841 acaagggcgg gcagcctctg gccggccgct ttgagatcgt ggatgagatg caggcgtcgg
      901 cgggggtgga tgcgacgctg ttttatgggg acgagcgcta tctcaccacc atccgcacgc
      961 cggacggcgc gcgcgcgtcg ggcacccctg cgcagccgga ggtcgtccag cgggtgctgc
     1021 gggacggcga ggattttttt tccgatcggg tgctggtcaa cggggtgccc tatttcgggt
     1081 attacatgcc cttgaccaag gcggacggca cggcggtggg gatgcttttt gtgggcaggc
     1141 cgcgcgcgca ggtgatgaag agcatcgacc ggaacatcct gcaggtctgc ctctcggagg
     1201 cgggtatcct cgccatagcc gtggggcttg cgatttatta cagccgcaag atggtctact
     1261 cactgaacaa gacggaaaaa tttctcggcg agatcgccag cggcgacctg accgcccata
     1321 tcgaccccta cctgctggag cggcaggacg aggtgggcga gatggggcgc tttgccgtgg
     1381 tgctgcagcg gtccatcgag gacctggtgg gcaaggaccc gctcaccggc ctgtacaacc
     1441 ggcgcagctg cgacgtggtg ctgcaaagcc tggctgagcg ggcgcagcgg ggcgggcgcc
     1501 cctttacggt ggtcatgggg gacatcgatt attttaaaaa ggtaaacgac gactacgggc
     1561 accaggcggg ggatgcggtg ctcaaggcgg tggcggagac gctgtccgcc catatggagc
     1621 ggcggggatt cgtcttccgc tggggcggcg aggagttttt gctcctctat gaggagatgg
     1681 acgagaaagc ggcgcttctg cgcctgaaaa ggctgcaggg cgagctggcg cgggccaagg
     1741 tggaatggca gggcacgccg atccgcgtca ccatgacgtt cggtgtggcc gacagcaccc
     1801 acagcccgca gccggaagcg ctgatccgtc tggcggatga aaacctttat ctcggcaagc
     1861 aggaggggcg ggaccgggtg gtctcggccc gggaggagag agaattatga ccttggggca
     1921 ttggggattt tcatggacgg ggctgctgtt tttggcgctg ctgatggcgc cgaacctgct
     1981 ctggaccaaa aacaagccca aaggctacag cgccgccggt gaggaccgcc gcctgctggc
     2041 cttggagcgg atcggggagg cggggaccgt ctgtacggcg ctggtgttcc gcgacaccaa
     2101 cccgggcccc tggtcggcct ggagcctttg gctggccgcg gcggcgctgc tgatgctgct
     2161 gtacgagtgc tggtgggcgc gctatttcaa aagcggcaag cagctgtctg atttttacag
     2221 ctcgtttttg ggggtgccgc tggccggggc caccctgccg gtggcggcgt ttgggctgct
     2281 gggggtctat ggccgcctgc tgccgctgct cttttttacc ctggtgctgg gggtggggca
     2341 catcggcatc cacctgcagc actaccgggc gctggcgcgg ggcaggggct gaaagggcgc
     2401 gcccggcgct tcgggtcacc cgccctcccg cacgccagag cggaagaggc gtggcccggt
     2461 gcagaaaaac aggggcgcgg gccggtccgg cgcgcgccgc ggaagaggag aggaacgcat
     2521 gcggccctat caacataagg tgcagtacta cgagaccgac cggat
//
LOCUS       DBMDYA010000433         2560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8781_length_2560_cov_1.742985, whole genome shotgun sequence.
ACCESSION   DBMDYA010000433 DBMDYA010000000
VERSION     DBMDYA010000433.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_2560_cov_1.742985"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="ACZPFK_10225"
     CDS             complement(<1..2010)
                     /locus_tag="ACZPFK_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1406730.1"
                     /translation="MERFARFIVRRRKAVLVLAVLLLIPSVLGAVGTYINYDILTYLP
                     PELDSMIGEKYLEDDFKTASTAMVTVENMPTADLLQLKDQLAAVPGVRKVLGASDLGD
                     VTIPKEMLPDDLRRFFYNDEGAQLLVVTFDDTSASASTMAALAQIKGLLRENCLLGGM
                     SAILQDTKALVDEEMPLYILCAVGCSLAVLFLSLKETVVPLIFMLGIAFPIVYNFGTN
                     LFLGQISYITEALATVLQLGVTMDFSIFLLHRFEEERQRRPAEEAMVKAVCNTFSSIA
                     GSSLTTVAGFLALCTMSLTLGRDIGLVMAKGVLLGVLSTVTILPALIMTFQKAIGRHI
                     HRTLIPKLTRVSHFVVRRYLPILAVFTLVFIPFAVAQAKTPVYYTLFDSLPQDMGSIV
                     GTERLKADFDMTTTHFIMVDEKLTGSEMKALSDELAAVDGVNQVLSYEKFLGAGIPQQ
                     IIPEEVSGIFHAGGHRMILANSSYESGSERQNNQLTELNAIVKRYDPAGVISGEGAMT
                     KDLIEVADTDFRNVSIASIAAVFAIIAVVFRSASIPVLLVAVIESAIMINMGIPYFTG
                     SVLPFVASIVIGTIQLGATVDYAILTTTRFREELSLGRTVKEAVQVAVEQCSQSILTS
                     GLTFFAATASVAVISRMELLRSLCLLISRGALISMLVILLVLPALL"
     gene            2233..>2560
                     /locus_tag="ACZPFK_10230"
     CDS             2233..>2560
                     /locus_tag="ACZPFK_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1406731.1"
                     /translation="MSSRLEQQKQEKKQNLLDAAYALFTEKGVAKTSVDEIVRGANVA
                     KGTFYLYFRDKGDVLRELIYKVSYRVLGEAYDYAVAHESGDFIEDVILLVDYSIEYFK
                     RNKLALR"
BASE COUNT          550 a          767 c          750 g          493 t
ORIGIN      
        1 gagcagcgcc ggcagcacca gcaggatgac caacatgctg atgagggcgc cgcggctgat
       61 gagcaggcag aggctgcgca gcagctccat gcgggagatg accgcaacgc tggcggtggc
      121 tgcaaaaaag gtcagcccac tggtgaggat gctctggctg cactgctcca ccgccacctg
      181 taccgcctct ttcaccgtgc gccccaggct cagctcctcc cggaagcggg tggtggtgag
      241 gatggcgtaa tccaccgtcg cccccagctg tatggtgccg atgacgatac ttgccacaaa
      301 gggcagcacc gagccggtga aataggggat gcccatgttg atcatgatgg cgctctcgat
      361 gaccgccacc aaaagcaccg ggatggaggc ggaccgaaag accactgcga tgatggcaaa
      421 gaccgcggcg atggaggcga tactcacatt gcggaaatcc gtgtccgcga cctcgatcag
      481 gtccttggtc atcgcgccct cgccgctgat gacccccgcc gggtcatacc gcttgactat
      541 ggcgttcagc tctgtgagct ggttgttctg ccgctctgag ccgctctcat agctggagtt
      601 cgccaggatc atgcggtggc cgccggcgtg gaagatgccg ctgacctcct ccgggatgat
      661 ctgctggggg atgcccgcac ccaaaaattt ttcatagctg agcacctggt tgacgccgtc
      721 caccgcggcc agctcgtcgc tgagggcttt catctcgctg ccggtcagct tttcgtccac
      781 catgatgaaa tgagtggtgg tcatgtcaaa atccgctttc agccgctcgg tgcccacaat
      841 gctgcccatg tcctggggca gactgtcgaa cagggtgtaa tagaccggcg ttttggcctg
      901 cgccacggca aaggggataa agaccagcgt gaagaccgct aaaatgggca ggtaccggcg
      961 caccacaaaa tgagagacac gcgtcagctt ggggatgagg gtgcggtgga tgtgcctgcc
     1021 gatcgctttt tgaaacgtca tgatcagcgc gggcaggatg gtgacggtgc tcagcacccc
     1081 caacagcacc cctttcgcca tcacaaggcc gatgtcgcgg cccagggtca gcgacatggt
     1141 gcaaagcgcc aaaaaaccgg cgacggtggt cagcgagctg cccgcgatgg aagaaaaggt
     1201 gttgcagacg gccttgacca tggcctcctc ggccgggcgc cgctgccgct cctcctcgaa
     1261 ccggtgcagc agaaagatgg aaaaatccat cgtcacaccc agctgcagca cggtggccaa
     1321 cgcctcggtg atataagaga tctggcccag aaagagattg gtgccgaaat tgtagacgat
     1381 gggaaaagcg atgcccagca tgaagatcag cggcaccacc gtctctttca agctcaaaaa
     1441 cagcaccgcc agcgagcagc ccaccgcgca caggatgtac agcggcatct cctcgtccac
     1501 cagggccttg gtgtcctgca aaatggccga catgccgccc agcaagcagt tctcccgcag
     1561 cagccccttg atctgcgcca gggcggccat ggtggaggcg ctggcgctgg tgtcgtcaaa
     1621 ggtcaccacc agcagctggg ccccctcgtc gttgtaaaaa aagcgccgca gatcgtcggg
     1681 cagcatctcc ttggggatgg tcacgtcccc caggtcactg gcccccagca ccttgcgcac
     1741 ccccggcacc gccgccagct ggtctttcag ctgcagcagg tccgccgtgg gcatgttttc
     1801 caccgtgacc atcgcagtgg aggcggtctt gaaatcgtcc tcaagatact tttcaccgat
     1861 catcgaatcc agctcgggcg gcagataggt gaggatgtcg tagttgatat aggtgcccac
     1921 cgcccccagc accgacggga tcagcagcag caccgccagc accagcaccg cttttcggcg
     1981 gcggacgatg aaccgtgcaa accgttccat gggatcatct cctttggtct tttcaaaact
     2041 gactatcagt caacaagcta cagtatagct cagctctgac cattggtcaa ttcttaaaat
     2101 gaccgatagt cattcatatt tcttgctgac ttttggtcat tttgctgaat cgcaacagcg
     2161 ctcttgtttt tcgccttttt tcaccctata ctaaaggcgt aagctcagtc ccttttgaga
     2221 ggaggcgcgc agatgagcag ccgtttggaa cagcaaaagc aggaaaaaaa gcagaatctt
     2281 ctcgacgcgg catatgccct gttcactgaa aagggggtcg ccaagacctc ggtggacgag
     2341 atcgtgcggg gcgccaacgt cgccaagggc accttttatc tctatttccg cgacaagggc
     2401 gatgtgctgc gcgagctcat ctacaaggtc agctaccggg tgctgggcga ggcctacgac
     2461 tacgccgtgg cccacgagag cggcgatttt atcgaggatg tcattttgct ggtggactat
     2521 agcatcgaat acttcaagcg caacaagctg gccctgcggc
//
LOCUS       DBMDYA010000434         2556 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8800_length_2556_cov_1.795927, whole genome shotgun sequence.
ACCESSION   DBMDYA010000434 DBMDYA010000000
VERSION     DBMDYA010000434.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8800_length_2556_cov_1.795927"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="xseB"
                     /locus_tag="ACZPFK_10235"
     CDS             <1..238
                     /gene="xseB"
                     /locus_tag="ACZPFK_10235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1406732.1"
                     /translation="ACRMKKKQSFEEMLDELNGMIARLSDDDTPLEEALKLYAQAAEK
                     IAACSGVLEHAKVQIEEIGKTMEQQTEGGRDEEL"
     gene            225..1109
                     /locus_tag="ACZPFK_10240"
     CDS             225..1109
                     /locus_tag="ACZPFK_10240"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1406733.1"
                     /translation="MKSYNEQYAAYVDALEERLELECGRRFLPGSAVGEAARYSLMAG
                     GKRVRGVLTLAFCDLLGGDGDAAAAFGAAVEMLHCYSLIHDDLPCMDDADTRRGQAAC
                     HKKFGEATALLAADALLTEAFEVIAASGAGDAQKAAAVACLAAAAGGRGMVYGQELDL
                     AFEKKQPSEQDLTRIHTHKTGDLIHAAGLLGVLASDHTARDEEECGAYTRAIGLVFQI
                     VDDVLDCTSTAQQMGKPVGNDAAAGKITFATLKGPEAAMLQAQQLTGQACDRLTGVYG
                     GRAAFLAELARSLAARRS"
     gene            1127..1600
                     /locus_tag="ACZPFK_10245"
     CDS             1127..1600
                     /locus_tag="ACZPFK_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1406734.1"
                     /translation="MQKLWSFLACNYILYVPLLSWLAAQICKTIIDLILSGKLDLERM
                     FGAGGMPSAHSALVCSMTIATARKFGVSSPFFAFAFILAAIVMYDAMGVRRAAGEQAK
                     VLNRIVDDWMNEEDDEENPLRENGKRLKEKVGHTPFEVLSGALLGILMAMIFPVT"
     gene            1616..>2556
                     /locus_tag="ACZPFK_10250"
     CDS             1616..>2556
                     /locus_tag="ACZPFK_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR1406735.1"
                     /translation="MQNILDKIISGELQLKELSADRLERLCAEIRGFLIEHVSKTGGH
                     LSSNLGTVELTVALHRVFTTPQDAIVFDVGHQCYTHKILTGRAPRFDTLRRAGGLSGF
                     PNREESPHDAFIAGHGNTALSAAIGIARAKKLKGEPGTVVAVVGDGAFTGGMVYEGMN
                     NIDTLDNLVVVLNDNKMSISKNVGSVSRYLTSLRTSPSYHTAKHRTEDILDKTPVVGR
                     YIKEALVYSKSVLRRSIYNSTFFEEMGFQYIGPLDGHNLEDLIAAFASAKEYDSPLFL
                     HVETVKGKGFTPAEQNPAAFHGVGAFDTRNLPDPDGP"
BASE COUNT          494 a          777 c          855 g          430 t
ORIGIN      
        1 agcttgccgg atgaagaaaa aacagagctt tgaagagatg ttggacgagc tgaacggcat
       61 gatcgcccgg ctctcggacg acgacacccc tctcgaggag gcgctcaagc tctacgcgca
      121 ggcggcggag aagatcgccg cctgcagcgg agtgctggag catgccaagg tgcagatcga
      181 ggagatcggc aaaaccatgg aacagcagac ggagggcggc cgcgatgaag agctataacg
      241 agcagtacgc ggcctatgtg gacgcgctgg aagagaggct ggagctggag tgcgggcgca
      301 ggtttttgcc gggctccgcg gtgggcgagg cggcgcgcta cagcctgatg gccgggggca
      361 agcgggtgcg cggcgttctc acactggcgt tttgtgatct gctgggcggc gacggagacg
      421 cggccgccgc ttttggcgcc gcggtggaga tgctgcactg ctattcgctg atccacgacg
      481 acctgccctg catggacgac gcggacaccc ggcgcggcca ggctgcgtgc cacaaaaaat
      541 tcggcgaggc cacggctttg ctggctgcgg acgcgctgct caccgaggcc tttgaggtga
      601 tcgcggcaag cggcgcgggg gacgcgcaga aagccgccgc ggtcgcctgt ctcgccgccg
      661 ccgccggggg ccgcggcatg gtctacgggc aagagctgga cctcgccttt gaaaagaagc
      721 agccctccga gcaggacctc acccgcatcc acacgcacaa gaccggcgat ctgatccatg
      781 cggcggggct gctgggggtg ctggcgtcgg accacacggc gagggacgag gaggagtgcg
      841 gcgcttatac ccgcgcgatc ggccttgtct ttcagatcgt ggacgacgtg ctggactgca
      901 cctccaccgc gcagcagatg ggcaagccgg tgggcaacga cgccgcggcg gggaaaatca
      961 cctttgcgac gctcaagggg ccggaggcgg ccatgctgca ggcgcagcag ctcaccgggc
     1021 aggcctgcga ccggctgacg ggcgtctacg gcggccgggc ggcgttcctg gccgagctgg
     1081 cacgctcgct ggccgcaagg agaagctgaa aagaggggcg gatcaaatgc aaaagctatg
     1141 gagttttctg gcgtgcaact acatcctgta tgtaccgctt ctttcctggc tcgccgcgca
     1201 gatctgcaag acgatcatcg acctgatcct cagcggcaag ctggacctgg agcggatgtt
     1261 cggcgccggg ggcatgccca gcgcccactc ggccctggtc tgctccatga ccattgccac
     1321 cgcgcgcaaa tttggggtgt cctcgccctt tttcgccttt gcgttcatcc tcgccgccat
     1381 cgtcatgtac gacgcgatgg gggtgcgccg cgccgcgggc gagcaggcca aggtgctcaa
     1441 ccgcatcgtg gacgactgga tgaacgagga ggacgacgag gaaaatcccc tgcgggaaaa
     1501 cgggaagcgc ctcaaggaaa aagtgggcca caccccgttt gaggtgctca gcggcgcgct
     1561 gctcggcatt ttgatggcga tgatctttcc cgttacatga taggagttgg aatacatgca
     1621 aaacatcctg gataagatca tatccggaga attgcagttg aaagagcttt ccgcagaccg
     1681 gctggaacgg ctgtgcgccg agatccgcgg ctttttgatc gaacacgtct ccaagaccgg
     1741 cggacatctg tcctcaaacc tcggcaccgt ggagctgacc gtggcgctgc accgggtctt
     1801 taccaccccg caggacgcca tcgtcttcga cgtggggcac cagtgctaca cccacaagat
     1861 cctcaccggg cgcgcgccgc gctttgacac cctgcgccgt gccggggggc tgtccggctt
     1921 ccccaaccgg gaggagagcc cccacgacgc ctttatcgcc gggcacggca acacggcgct
     1981 gtcggccgcc atcggtatcg cccgggcgaa aaagctgaaa ggggaaccgg gcaccgtggt
     2041 tgcggtggtc ggggatggcg cttttaccgg cggcatggtc tacgagggca tgaacaacat
     2101 cgataccctc gacaatctgg tggtggtgct caacgacaac aagatgtcca tctccaagaa
     2161 cgtgggttcc gtttcccgct atctcaccag cctgcgcacc agcccgtctt accacacggc
     2221 caagcaccgc accgaggata ttttggacaa gacgccggtg gtggggcgct atatcaagga
     2281 ggcgcttgtc tactccaaat ccgtgctgcg gcgcagcatt tacaattcga ccttttttga
     2341 ggagatgggc tttcagtaca tcggcccgct ggacgggcat aacctggagg acctgatcgc
     2401 ggcttttgcc agcgccaaag agtacgacag cccgcttttt ctgcatgtgg agaccgtcaa
     2461 gggaaagggc tttactccgg cggagcaaaa cccggccgcg ttccacggcg tgggggcgtt
     2521 cgacacccgc aacctgccgg acccggacgg gccgat
//
LOCUS       DBMDYA010000435         2547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8837_length_2547_cov_1.192968, whole genome shotgun sequence.
ACCESSION   DBMDYA010000435 DBMDYA010000000
VERSION     DBMDYA010000435.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_2547_cov_1.192968"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="ACZPFK_10255"
     CDS             <1..1957
                     /locus_tag="ACZPFK_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1406736.1"
                     /translation="GYYTEEHILICLSSSPSNAKVIRTAARMAEAFHGSFTALFVETP
                     GTTELDEGNRDRLRRNLRLAEQLGARIATVYGENVARQIADYARESGVSKIVLGRSAP
                     GGRGVLARPGLVEQLTALAPNLDIYIIPDTRPPYQPRRHGAARPAWSAADLLKSAGIL
                     AATSLLALLFRRWGFTEANIVTLYILGVLFISMCTHSWAYGAASSAAGVLVFNYLFTE
                     PLLTLKFNDKSYYVTFVVMFLASFITGTLTTRVRRQAQQAASKAYRTEILLETSQKLQ
                     HSETEAQIFAEMGGQLLRLVHRTVLLYPVREGALGEPQVFLPPGAQPAESCDTADERA
                     VAAWVAKNNKHAGATTDTLPGARCLYLAVRGRDAVFAVAGLALEGEPLEIFERNLLIA
                     MLGEFGLAVEKQRATEEKRKFAVEAEQERLRADLLRAISHDLRTPLTSISGNAGILMA
                     NGKVLDEGKRQQLYTDIYDDAMWLINLVENLLAVTRIENGTMDIQGEPELVNDVVEEA
                     LAHVDRKLSLHRLALDLPDELLMARMDARLIVQVLINLVDNAVKYTPPGSTITVGARR
                     DGPMVRIEVADDGPGVPDEAKERLFDMFYTAQGKRGDGRRGLGLGLSLCRSIVNAHGG
                     DISVRDNQPAGTVFSFTLKAEEVTCHE"
     gene            1950..>2547
                     /locus_tag="ACZPFK_10260"
     CDS             1950..>2547
                     /locus_tag="ACZPFK_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1406737.1"
                     /translation="MNRPQILIVEDDKAVRNLIATTLETREYAYHTAENGAAALLEAV
                     SRPPDVMILDLGLPDMDGVEIIKKVREWSSLPIIVVSARSEESDKIGALDAGADDYLT
                     KPFSPEELLARLRVAIRRSRYQQAAPGEAASVYKNGELVIDYAAGCASMAGREIHLTP
                     IEYRLLCLLARNTGRVLTHNYILREVWGSALESDIPSLR"
BASE COUNT          437 a          859 c          868 g          383 t
ORIGIN      
        1 cggctattac accgaggagc atatcctcat ctgcctttcc agctcgccct ccaacgccaa
       61 ggtgatccgc accgcggccc gcatggccga ggcctttcac ggcagcttca ccgccctgtt
      121 cgtcgagacc cccggcacca ctgagctgga cgagggcaac cgggaccggc tgcgccgcaa
      181 cctgcgtctg gccgagcagc tgggcgcgcg catcgccacg gtctacggcg aaaacgtggc
      241 ccggcagatc gccgactacg cgcgggagag cggggtgtcc aagatcgtgc tgggccgctc
      301 cgcccccggc gggcgcgggg tgctggcgcg ccccggcctg gtggagcagc tcaccgccct
      361 ggcgcccaat ctggatatct atatcatccc ggacacccgc ccgccgtatc agccgcggcg
      421 gcacggcgcg gcgcgcccgg cctggagcgc cgccgacctg ctcaagtcgg cgggcatcct
      481 ggcggccacc tcgctgctgg cgctgctgtt ccggcgctgg ggcttcaccg aggccaacat
      541 cgtcacgctg tatatcctgg gggtgctctt catctccatg tgcacccaca gctgggccta
      601 cggcgcggcc tcctcggcgg cgggggtgct ggtcttcaac tatctgttca ccgaaccgct
      661 gctcaccctc aagttcaacg acaaaagcta ttacgtcacc tttgtggtga tgttcctggc
      721 ctcctttatc accggcaccc tcaccacgcg ggtgcgtcgg caggcgcagc aggccgcctc
      781 caaggcctac cgcaccgaga ttttgctcga gaccagccaa aagctgcagc acagcgagac
      841 cgaggcgcag atctttgccg agatgggcgg ccagctgctg cgcctggtgc accggacggt
      901 gctgctctac ccggtgcggg agggggcgct gggcgagccg caggtctttc tgccgcccgg
      961 ggcacagccg gcggaaagct gcgacacggc cgacgagcgg gcggtggccg cctgggtggc
     1021 gaaaaacaac aagcacgccg gcgccaccac cgacacgctg cccggcgccc gctgcctgta
     1081 tctggcggtg cgcggccggg acgcggtctt tgcggtggcc ggtctggcgc tggagggcga
     1141 gccgctggag atctttgagc gcaacctgct catcgccatg ctgggcgagt tcggcctggc
     1201 ggtggaaaaa cagcgcgcca ccgaggaaaa gcgcaagttt gccgtggagg ccgagcagga
     1261 gcggctgcgc gccgatctgc tgcgggccat cagccacgac ctgcgcaccc cgctgaccag
     1321 catctcgggc aacgcgggca ttttgatggc caacggcaag gtgctggacg agggcaagcg
     1381 tcagcagctt tataccgata tctacgacga cgccatgtgg ctgatcaatc tggtggaaaa
     1441 cctgctggcc gtcacccgca tcgaaaacgg caccatggac atccaggggg agccggagct
     1501 ggtcaacgac gtggtggaag aggccctcgc ccatgtggac cgcaagctga gcctgcaccg
     1561 cctggcgctg gacctgcccg acgagctgct gatggcccgc atggacgcgc ggctgatcgt
     1621 gcaggtgctg atcaatctgg tggacaacgc cgtcaagtac accccaccgg gcagcaccat
     1681 cacggtcggc gcccggcggg acgggccgat ggtgcgcata gaggtggcgg acgacgggcc
     1741 cggtgtgccc gacgaggcaa aagagcggct gttcgacatg ttctataccg cccagggcaa
     1801 gcgcggggac ggccgccgcg gcctggggct gggcctgtcg ctctgccgct ccatcgtcaa
     1861 cgcccacggg ggcgacatct cggtgcggga taaccagccg gccgggacgg tgttttcctt
     1921 tacattaaaa gcggaagagg tgacctgcca tgaatagacc gcagatcctc attgttgagg
     1981 acgacaaggc ggtgcgcaac ctgatcgcca ctaccctgga gacgcgggag tacgcctacc
     2041 acacggcgga gaacggcgcg gcggcgctgc tggaggccgt gtcccggccg cccgacgtga
     2101 tgatcctgga cctggggctg cccgacatgg acggggtgga gatcatcaaa aaggtgcgcg
     2161 agtggagcag cctgcccatc atcgtcgtca gtgcccgaag tgaggagagc gacaagatcg
     2221 gcgcgctgga cgccggcgcg gacgactacc tgaccaagcc tttcagcccc gaggagctgc
     2281 tggcccggct gcgggtggcc atccgccgca gccgttacca gcaggccgcg ccgggcgagg
     2341 cggccagcgt ctacaaaaac ggcgagctgg tcatcgacta cgcggcgggc tgcgcctcaa
     2401 tggcgggcag ggagatccac ctcaccccca ttgaataccg gctgctctgc ctgctggccc
     2461 gcaacacggg ccgggtgctc acccacaact acatcctgcg ggaggtgtgg ggcagcgcgc
     2521 tggagagcga catcccctcg ctgcggg
//
LOCUS       DBMDYA010000436         2538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8871_length_2538_cov_1.784571, whole genome shotgun sequence.
ACCESSION   DBMDYA010000436 DBMDYA010000000
VERSION     DBMDYA010000436.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_2538_cov_1.784571"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="ACZPFK_10265"
     CDS             <1..245
                     /locus_tag="ACZPFK_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HIR1406738.1"
                     /translation="SEILILDCPTRGIDVGVKAAIYRLMEELKAQGKAILMISEELPE
                     VIGMSDRILVLKDGEVQKEFLRGPDVTESVIIEYMI"
     gene            260..1243
                     /locus_tag="ACZPFK_10270"
     CDS             260..1243
                     /locus_tag="ACZPFK_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406739.1"
                     /translation="MNEAATKERKGNALLALWKDKERRGKLIRSLVPFVGLIFIIVFF
                     QITTNGRLLMVKNLKTVLNQTFSIILGACGMVFVISQGNLDFSMGSVSGMAAALAAMV
                     CGAGPLVSLAVALVIGAAIGTLNGLMHVGFKAPANIVTLCTQFTFRGVVQVITATGLI
                     IPMAWSWMDGIPVKIVVSIVLVLITIVVFEYTKFGKYCKAIGSGGAASIQSGVPLNKM
                     KVLAFLLSGVAAGLCGFFSMIRAGSVAPTTGTGLEMNVLLAIVLGGMPLSGGASSKVR
                     AVILGSAMLAFLMNGLVIWGLNDLVQQGVKGAIFLVAVALSFERGNIAVIK"
     gene            1303..2505
                     /locus_tag="ACZPFK_10275"
     CDS             1303..2505
                     /locus_tag="ACZPFK_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1406740.1"
                     /translation="MKKLLALALTLTMALTLLAGCGGSDSSSGAPANDGTSTASSLSA
                     AVSAKGEKVKMGILIWGTTDALGRNSTMMVQKLVDQVGGEVVIDSSYTSPETQIQSAE
                     NLIAGGCNAILIVNSSDTMLPSLAKLCEENEVYFGLQWRRVVSDEIKAELDKCPYFVG
                     NTCEDETEIATRLATNLANAGVTQLAMIGRPVGDTTHDARWQGVVNVCDSTDMELVAE
                     YRGQSGAASAAETMEAVEKFITGYPDLEAIFLTGGTSSQLEGALAALDKHNKRGEIKL
                     AVVDFIDADQMKEYLEDGTLASIAGGHYVDPIFTAAMLINAVEGNKLSDKPEQIDLQF
                     IDFQSVNDALDYYDYVENDADGIYAYTEEELASMIKSLNPDLTIDSLRQMAADYSIED
                     VMERHGNA"
BASE COUNT          555 a          638 c          736 g          609 t
ORIGIN      
        1 gatcggaaat attgattctt gactgtccca cccgcggtat cgatgtgggg gtcaaggccg
       61 ctatttatcg cttgatggaa gagctcaagg cccagggcaa agcgattctt atgatctcgg
      121 aggagctgcc ggaggtcatc ggcatgagtg accgcatctt ggtactcaag gacggagagg
      181 tgcaaaagga gttcttgcgc ggccccgacg tcaccgagtc tgtgatcatc gagtacatga
      241 tttaaggagg gccgcaaaaa tgaacgaagc tgcaacaaaa gagagaaagg gaaatgccct
      301 gctcgctctc tggaaagaca aggagcgtcg cggtaaactg atccgttcgt tggttccgtt
      361 tgtagggttg atttttatca ttgtcttttt ccagatcact accaacggac gcctactgat
      421 ggtaaaaaat ttaaaaactg tgctcaacca gaccttttcc attattctcg gcgcctgcgg
      481 tatggtgttc gtcatttccc aggggaatct ggatttttcg atgggttctg tctccggtat
      541 ggctgccgcg ctggctgcga tggtctgcgg cgcgggaccc ctggtctctt tggcagtggc
      601 gcttgttatc ggcgctgcga ttggcaccct gaatggtttg atgcacgtgg gatttaaggc
      661 cccggccaat attgtcaccc tctgcactca gtttaccttt cgcggggtgg tgcaggtcat
      721 cacagcgact ggcctgatca tccctatggc ttggagttgg atggatggaa ttccagttaa
      781 aattgtagtg tccatagtat tggtcctgat taccatcgtg gtattcgagt acaccaaatt
      841 cggcaaatac tgcaaggcca tcggttcggg gggcgcggct tcaattcagt ccggcgtacc
      901 gctgaacaag atgaaagtac tggctttctt gctctctggc gttgcggccg gcctgtgcgg
      961 atttttctct atgattcgtg ccggctcagt ggcccccacc actggcacag ggctggaaat
     1021 gaatgtattg ctggccattg tgctgggcgg tatgccgctt tccggtggcg ccagttccaa
     1081 ggtgcgggcg gtgattctgg gcagtgctat gctggccttt ctgatgaacg gtttggtaat
     1141 ctggggcctg aacgacctgg tacagcaggg cgtcaaaggg gcaattttcc tggtcgcagt
     1201 cgccctttct tttgagcgtg gcaacatcgc ggtcatcaaa taacttcgca atcgtttttg
     1261 agggcccttt taaaaggctc tgcaataaaa ggaggtaaag atatgaaaaa attattggca
     1321 ctggctttga cgctgaccat ggcgttaacc ctgctcgccg gttgcggcgg cagcgactcc
     1381 tcttctgggg cccccgcaaa tgatggcacc agcaccgctt ccagcctgag cgcagccgtt
     1441 tcggctaagg gtgaaaaagt caagatgggg atcctcatct ggggcaccac ggacgctttg
     1501 ggccgcaatt ctaccatgat ggtgcagaaa ctggtcgatc aggtgggcgg cgaggtcgtc
     1561 atagattcca gctatacttc cccggaaacc cagatccagt ccgctgaaaa cctgattgca
     1621 ggcggctgca atgccatcct gatcgtcaac agctctgaca ccatgctgcc cagcttggct
     1681 aagctttgcg aggagaacga ggtctatttt ggtcttcagt ggcgccgtgt ggtctcagat
     1741 gaaattaagg cggagcttga taaatgcccg tattttgtgg gcaacacctg tgaagacgaa
     1801 actgagatcg cgacccgcct tgcaacgaat ttggcaaatg ccggtgtcac ccagctggct
     1861 atgattggcc gtcctgtagg cgataccacc catgatgcgc gttggcaggg cgttgtgaat
     1921 gtctgcgatt ccaccgatat ggagctggtc gccgagtacc gcggccagag cggcgcagcg
     1981 agtgcggctg agaccatgga agcggtggag aaattcatca ccggttaccc ggatctggaa
     2041 gccattttcc tgaccggcgg cacgagctct cagttggagg gcgcattggc tgccctggac
     2101 aagcacaata aacgcggcga gatcaaattg gcagttgtgg acttcattga cgcggatcaa
     2161 atgaaagagt acctggagga tggcactctg gcctcgatcg cgggcggaca ttatgtcgac
     2221 ccgatcttca ccgccgctat gctgatcaac gcggttgagg gcaacaagct ctctgacaag
     2281 cccgagcaga tcgacctgca gtttatcgat ttccaatccg tgaacgacgc gttggattac
     2341 tatgattatg tcgagaacga tgctgatggc atctacgcct atactgagga agaactggct
     2401 tctatgatca agagcttgaa ccctgacctg accattgaca gcctgcgcca gatggccgca
     2461 gattacagca tcgaagacgt catggagcgg cacggtaacg cgtgaccgac cgtctcagaa
     2521 acagccggtc acacattt
//
LOCUS       DBMDYA010000437         2538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8872_length_2538_cov_1.673369, whole genome shotgun sequence.
ACCESSION   DBMDYA010000437 DBMDYA010000000
VERSION     DBMDYA010000437.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8872_length_2538_cov_1.673369"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2307)
                     /locus_tag="ACZPFK_10280"
     CDS             complement(<1..2307)
                     /locus_tag="ACZPFK_10280"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HIR1406741.1"
                     /translation="MYKVVKRDGTLAEFNISKISAAITKAFEALEKQYHPSVIDLLAL
                     KVTSDFEPKIKDGCIQVEDIQDSVEEVLSLAGYADVAKAYILYRKQREKVRNVKSSLL
                     DYKKLVGDYLNVNDWRVKENSTVTYSVGGLILSNSGAITANYWLSEIYDEEIAEAHRS
                     AAIHLHDLSMLTGYCAGWSLRQLIEEGLGGVPGKITSSPAKHLASLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFVKVDHLTYYEVKKCIESFIYGVNTPSRWGTQAPFSNITL
                     DWTVPPDLAEQPAIVGGKPMDFKYKDCKAEMDMVNKAFIEIMIQGDAEGRGFQYPIPT
                     YSITKDFDWSDTENNRLLFEMTAKYGTPYFSNYINSDMEPSDVRSMCCRLRLDLRELR
                     KKSGGFFGSGESTGSVGVVTINLPRIAYLAKDEADFYKRLDRLMDVSARSLHIKRTVI
                     TKLLNEGLYPYTKRYLGSFDNHFSTIGLLGMNEAGLNAKWLGEDLTHPETQKFAKDVL
                     NHMRERLSDYQEEYGELYNLEATPAESTSYRLAKHDKARYPDIVTAGDSDGAPYYTNS
                     SHLPVGYTADIFEALDVQDELQTLYTSGTVFHAFLGEKLPDWKAAATLVRTIAENYTL
                     PYYTLSPTYSICKHHGYLAGEQYTCPECGGRTEVYSRITGYYRPVQNWNDGKTQEFKE
                     RKVYDIGNSHLKEGHKPAANTVTVPQKPAAGNGARVLLFTTSTCPKCKVAGAMLDSAA
                     LPFEKIVADQSGNEELARAYGISQAPTLV"
BASE COUNT          415 a          740 c          829 g          554 t
ORIGIN      
        1 caccagggtg ggcgcctggc tgatgccgta ggcgcgcgcc agctcctcgt tgcccgattg
       61 gtcggccacg atcttttcaa acggcagggc tgccgaatcc agcatcgcgc cggccacctt
      121 gcacttgggg caggtggagg tggtgaacag cagcacccgg gcgccgttgc ccgccgccgg
      181 cttctgcggc acggtgacgg tgttggcggc cggcttgtgc ccctctttca gatgggagtt
      241 gccgatgtcg tacaccttgc gctctttgaa ctcctgggtc ttgccgtcgt tccagttctg
      301 caccgggcgg tagtagccgg tgatgcggct gtagacctcg gtgcggccgc cgcactccgg
      361 gcaggtgtac tgctcgccgg ccagatagcc gtgatgcttg cagatggaat aggtgggcga
      421 cagggtgtag tagggcaggg tgtaattttc ggcgatggtg cgcaccaggg ttgcggccgc
      481 tttccaatcc ggcagctttt cgcccaggaa agcgtggaac accgtgcccg aggtgtagag
      541 ggtctgcagc tcgtcctgca cgtccagcgc ctcaaagatg tcggcggtat agcccaccgg
      601 caggtgggag ctgttggtgt agtagggcgc gccgtcgctg tcgccggcgg tgacgatatc
      661 cgggtagcgg gccttgtcgt gctttgccag ccggtagctg gtggactcgg cgggggtggc
      721 ctccaggttg tacagctcgc cgtactcctc ctgatagtcg ctcaggcgct cgcgcatgtg
      781 gttgagcacg tccttggcga acttctgggt ctcggggtgg gtgaggtcct cgcccagcca
      841 cttcgcgttg aggcccgcct cgttcatgcc cagcaggccg atggtggaga agtggttgtc
      901 gaacgagccc agataccgct tggtgtaggg gtacaggccc tcgttcagca gcttggtgat
      961 gacggtgcgc ttgatgtgca gcgagcgggc ggagacgtcc atcagcctgt caagccgctt
     1021 gtagaaatcc gcctcgtcct tggcaagata cgcgatgcgg ggcaggttga tggtgaccac
     1081 gcccaccgag ccggtgctct cgccgctgcc gaagaagccg ccggattttt tgcgcagctc
     1141 gcgcagatcc agccgcaggc ggcagcacat gctgcgcacg tcgctgggct ccatatcgct
     1201 gttgatgtag ttggagaaat agggggtgcc gtacttggcg gtcatctcga acagcagccg
     1261 gttgttctcg gtgtcggacc agtcgaaatc cttggtgatg gaataggtgg ggatggggta
     1321 ctggaagccg cgcccctcgg cgtcgccctg gatcatgatc tcgatgaacg ccttgttgac
     1381 catgtccatc tcggccttgc agtccttata cttgaagtcc atcggcttgc cgcccacgat
     1441 ggcgggctgc tcggccagat cgggcggcac cgtccagtcc agcgtgatgt tggaaaacgg
     1501 ggcctgggtg ccccagcggc tgggggtgtt gacgccgtag atgaaagact cgatgcactt
     1561 tttgacctcg taataggtca ggtgatccac cttgacaaag ggcgcgaggt aggtgtcgaa
     1621 agaggagaac gcctgcgcgc ccgcccactc gttctgcatg atgcccaaaa agttgaccat
     1681 ctggttgcac aggctggcca gatgcttggc cggtgaggag gtgatcttgc cgggcacgcc
     1741 gcccagcccc tcctcgatca gctgccgcag cgaccagccc gcgcagtagc cggtgagcat
     1801 cgacaggtcg tgcagatgga tggcggcgct gcggtgggcc tcggcgatct cctcgtcgta
     1861 gatctcgctg agccagtagt tggcggtgat ggcgccgctg ttgctcagga tcagcccgcc
     1921 caccgaatag gtgacggtgg agttctcttt gacgcgccag tcgttgacgt tcaggtaatc
     1981 gcccaccagt tttttatagt ccagcaggga ggatttgacg ttgcgcactt tctcgcgctg
     2041 cttgcggtag aggatatagg ccttggccac gtccgcgtag cccgccaggc tgagcacttc
     2101 ctccacgctg tcctggatat cctccacctg gatgcagccg tccttgatct tgggctcaaa
     2161 gtcagaggtg accttgagcg ccagcaggtc gatcacactg ggatgatact gcttttcaag
     2221 cgcctcgaac gccttggtga tagccgcgct gatcttgctg atgttgaact ccgcgagggt
     2281 gccgtccctc ttcactacct tgtacatgtt cctgcctgcc tcgattcttt gaattctgct
     2341 ctccgccccg ccgcggcggc cgcccgcctc cgggcgccgc ccgacggaca aaaaaagaga
     2401 gacccgccgg gacctctggc gccctttggc gcgctgggag aatgcctccc taaaaggata
     2461 acacaggggt ccctgcaagt caatattttg tgtgatatat tttgatttat actagatata
     2521 gttatacgcc gcgtattt
//
LOCUS       DBMDYA010000438         2523 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8925_length_2523_cov_1.748555, whole genome shotgun sequence.
ACCESSION   DBMDYA010000438 DBMDYA010000000
VERSION     DBMDYA010000438.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8925_length_2523_cov_1.748555"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="ACZPFK_10285"
     CDS             <1..682
                     /locus_tag="ACZPFK_10285"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1406742.1"
                     /translation="VRNSIGYPHLNVKIGATHGGLAVGEDGATHQCIEDFALMRAIPG
                     MTVVCPADDAEAQAAVRAAAALEGPVYLRFGRDPVPVLHDVLPRFELGRALLLRPGSD
                     VTIAATGPLVSIALDAAVLLAGRGVSARVLDFHTIKPIDVEMLTLASAETGAIVTAED
                     HNIVGGLGSAVCEALAQRLPVPVLRVGVEDKFGMSGHGPELYEAYGLTAEHIAAACAD
                     AIALKRRG"
     gene            complement(1097..1363)
                     /locus_tag="ACZPFK_10290"
     CDS             complement(1097..1363)
                     /locus_tag="ACZPFK_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406743.1"
                     /translation="MEKEIWIESFDYFKAGNRFAGSSGAFCFKIFPEKEEARLRVDTW
                     QGPNCCEKTEEKEERSFELSDEGLRQAVEWINEQNKSFQAQRGG"
     gene            1667..2506
                     /locus_tag="ACZPFK_10295"
     CDS             1667..2506
                     /locus_tag="ACZPFK_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1406744.1"
                     /translation="MDKQEAYVLIEGLKVVFDIVRLVDVTTTTQYTLTRDGRLIAEPY
                     ECYAVWHKDRRCENCVSAKAFAKKGQLAKYEFIGKDVYYVMSKYVLVDEQPYMLEMVS
                     RVTDETLFAAYGKSEFVNTIEGLNKKLYTDPLTGAYNRRYYNDQLCGLLSAANSFALV
                     DVDRFKQVNDSFGHPAGDLALKAVAGVLLCGLRRTDAVIRYGGDEFFLVFHDIPRQAL
                     AKKLEAMRERVAGIVVKEYPSLRLSVSIGATCGFDDPDEVLKRADKALYQAKKRRDCV
                     CVL"
BASE COUNT          392 a          859 c          815 g          457 t
ORIGIN      
        1 ggtgcgcaac tccatcggct atccccacct caacgtcaag atcggcgcca cccacggcgg
       61 cctggcggtg ggggaggacg gcgccaccca ccagtgcatt gaggatttcg cgctcatgcg
      121 ggccatcccg ggcatgacgg tggtttgccc tgcggacgac gcggaggcgc aggcggccgt
      181 gcgggccgcc gcggccctgg agggcccggt gtacctgcgc ttcggccgcg acccggtgcc
      241 ggtgctgcac gacgtgctgc cccggtttga gctgggccgc gccctgctgc tgcggccggg
      301 cagcgacgtg accatcgccg ccaccgggcc gctggtgtcc atcgcgctgg acgccgccgt
      361 gctgctggcc gggcgcgggg tatcggcgcg ggtgctggac tttcacacca tcaagcccat
      421 cgacgtggag atgctgaccc tggcctccgc cgaaaccggc gccatcgtca cggcggagga
      481 ccacaacatc gtgggcggcc tgggctccgc cgtctgcgag gccctggcgc agcgcctgcc
      541 ggtgccggtg ctgcgggtcg gggtggagga caagttcggc atgtccggcc acgggcccga
      601 gctgtatgag gcctacggcc tgaccgcgga gcacatcgcc gcggcctgtg ccgacgccat
      661 cgccctcaaa cgccgcggct gaggccgttc cctgtacttc cgccggggcg ggcggcggcc
      721 gcaaaaggcc cggcccggcg gcgctctttt gccagcgcgg tgtcgatcgg gaaccgcgcc
      781 cgcctggttc cggcgggcaa ggccctccgc cgcgacgacg cctctgcggg ccccgtcggc
      841 gcgctgcctc tctccatgtg tgggcggttt taccctcccg cacagtcccg ccccctgcgg
      901 gccctatcgg cccggggcgc gcggccgccc tgggccgggg ccttttccgc aggcagccga
      961 tgccccgcag gcttttcccg gcggccaaaa tcaaatcatc catcctcagc gcttttgtgc
     1021 gttttctccc tttttcatgc agaaagccgc cctctcacga gggcggcttt ctgttcgatt
     1081 cccgtttgtt ctgctctcag ccgccgcgct gcgcctgaaa gcttttgttc tgctcgttga
     1141 tccactccac cgcctgccgc agcccctcgt cggagagctc gaagctccgc tcctctttct
     1201 cctcggtctt ttcgcagcag ttggggccct gccaggtgtc gacccgcaga cgggcctcct
     1261 ctttttccgg aaaaatttta aagcagaacg cgccgctgct gccggcaaag cggttgccgg
     1321 ccttgaaata atcaaagctc tcgatccaga tctccttttc cacgtcggcc ctccctcact
     1381 gtgcgcccct gcgccgatgc tttcagttta ccaccaatgc ccgcccgcgc acaaggggcc
     1441 gccgcccgca cccgccccct gcggaccgaa aggcgtcgtc atttgtcttc cgtccgcgtt
     1501 cgttatataa ataaacggcg gaggagcgcg cctgcgcgcc gccgttcaga ggcaggccgg
     1561 tgaggaacgg cataccatga gagcggcgat tactttttac agcagcggcg cggcgcccgg
     1621 catcttgcct ggcctgcgcc gctgggaact gttggaggag agacggatgg acaaacagga
     1681 ggcctatgtc ctcatcgagg ggctgaaggt cgtctttgat atcgtgcggc tggtggacgt
     1741 caccaccacc acccaatata ccctcacccg cgacggccgg ctgatcgcgg agccctatga
     1801 gtgctacgcg gtctggcaca aggaccggcg ctgcgagaac tgcgtctcgg ccaaggcgtt
     1861 tgccaaaaag gggcagctgg ccaagtatga gttcatcggc aaggacgtct actatgtgat
     1921 gtccaaatat gttttggtgg acgaacagcc ctatatgctg gagatggtct cccgggtcac
     1981 cgacgagacc ctgtttgcag cctatggaaa aagcgagttt gtaaacacca tcgaggggct
     2041 gaacaaaaag ctttataccg acccgctgac cggcgcttac aaccggcgct attataacga
     2101 ccagctctgc ggcctgctga gcgccgccaa cagcttcgcg ctggtggacg tggatcgctt
     2161 caagcaggtg aacgacagct tcggccaccc ggcgggcgac ctggcgctga aagccgtggc
     2221 cggagtgctg ctctgcgggc tgcggcgcac cgacgcggtc atccgctacg gcggcgacga
     2281 gttttttctc gtctttcacg atatcccccg ccaggcgctg gcgaaaaagc tggaggcgat
     2341 gcgcgagcgg gtggcgggca tcgtggtgaa agagtatccc agcctgcgcc tctcggtgag
     2401 catcggcgcc acctgtggct tcgacgaccc ggacgaggtg ctgaagcggg ccgacaaagc
     2461 cctctaccag gccaaaaaga ggcgcgactg cgtctgcgtg ctctgaggcg cgggcgcggc
     2521 ttg
//
LOCUS       DBMDYA010000439         2520 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8944_length_2520_cov_1.403059, whole genome shotgun sequence.
ACCESSION   DBMDYA010000439 DBMDYA010000000
VERSION     DBMDYA010000439.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8944_length_2520_cov_1.403059"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="ACZPFK_10300"
     CDS             <1..690
                     /locus_tag="ACZPFK_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406745.1"
                     /translation="RGEMGLTGPQGPEGPMGPEGPMGLQGPAGERGPVGPMGEMGLTG
                     PQGPEGPMGPQGIEGPAGPQGPAGERGPQGPVGPQGPQGAAGTCPRVYAEYQFYDQCQ
                     DDATYIPLFRYFWNGSGIDLQSDNVTVKLAQGGAYLFSYSASATVSAGGYLKITPVIG
                     TSNEIPYSFTAHSQSSAVAITAANTFLFYAPVDIYVRLKVNASESVRLFGFLDIIRIA
                     DYRWPPDEEQG"
     gene            919..1278
                     /locus_tag="ACZPFK_10305"
     CDS             919..1278
                     /locus_tag="ACZPFK_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR1406746.1"
                     /translation="MKKVISRCPVCDSELTVTRLKCASCDTVIENNFRLSKFDYLSEE
                     ELYFTETFLRCRGNIKEVEKELGISYPTVRSKLDAVIKKLGYDAGADSQASRREEVLK
                     ALENGEITAEQAIAQLK"
     gene            1296..1673
                     /locus_tag="ACZPFK_10310"
     CDS             1296..1673
                     /locus_tag="ACZPFK_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR1406747.1"
                     /translation="MDEKLRILKMVEDGTVTAEQGAELMAALDAVPAEAALRSCYDKK
                     MFRILVDDVSGDKVKIQFPVGAIKKILKVTGKLPIPEKDLQGVDLSAMMDAVSECLED
                     EIEGDFVNVESADGTTVRVFVDQ"
     gene            1694..1957
                     /locus_tag="ACZPFK_10315"
     CDS             1694..1957
                     /locus_tag="ACZPFK_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406748.1"
                     /translation="MKVKIRTKELHFSMPLPVSMIGFVVRLIPAGVFDKMAADTPEPY
                     RCLVTKETVTMVLESCLDVLKENRGLEAVHVEAHDGTFVSIRL"
BASE COUNT          564 a          718 c          733 g          505 t
ORIGIN      
        1 aggggcgaga tgggcctcac cggtccgcag ggacccgagg gtcccatggg cccggagggt
       61 ccgatgggct tgcaggggcc cgcaggcgag cgcggccccg tcggccccat gggcgagatg
      121 ggcctcaccg gcccgcaggg acccgagggg ccgatgggcc cacaaggtat tgagggcccc
      181 gccggtcccc aagggcccgc tggagagcgg ggtccgcaag ggcctgtcgg cccccaaggg
      241 ccgcaggggg cggccgggac ctgcccccgg gtctacgccg agtatcagtt ctatgatcag
      301 tgccaggacg acgccaccta tatcccgctg ttccgctatt tctggaacgg cagcggcatc
      361 gacctgcagt cggacaacgt cactgtaaaa ctggcgcagg gaggggctta tctgttcagc
      421 tactccgcca gcgccaccgt atccgcgggc ggatatctca agatcacccc cgtgatcggc
      481 acctcgaacg agatccccta ttctttcact gcgcatagcc agagcagcgc cgtggcgatc
      541 accgcggcaa acacgttcct gttctacgcg cccgtggaca tctacgttcg cctcaaggtg
      601 aacgcatctg aaagcgtgcg gctgttcggg ttcctcgaca tcatccgcat tgcggactac
      661 cgctggccgc cggacgaaga gcagggctga cccccctctc tccgctctca aattcatcaa
      721 agggccgcct tttgaagggc ggcccttttt gcggtttgct ctcgcctttg ccgccttttt
      781 tctccggctc gcttttcgca aaaatatttt tatttaaaaa cttgaaaaaa ttaatattta
      841 tattaaaaat atcaatatta atattgacat attttatttt tatgttatat tgttgacaga
      901 cagaaaggaa gtggatgcat gaaaaaagtg atcagccgct gtccggtatg cgacagtgaa
      961 ctgactgtga cccggctcaa atgcgcttcc tgcgacacgg tgatcgaaaa caatttccgg
     1021 ctcagcaagt tcgactatct ctctgaggag gagctctact tcaccgagac ctttctccgc
     1081 tgccgcggca atatcaaaga ggtggaaaaa gagctgggca tctcctaccc caccgtccgc
     1141 tccaaactgg acgcggtaat caaaaagctg ggctatgatg cgggggcgga cagccaggcg
     1201 agccgacggg aagaggtttt aaaggccctt gaaaacggtg agatcacggc cgagcaggcg
     1261 atcgcacagc tgaaataaca aaaaggagac caatcatgga cgaaaaattg aggatcttga
     1321 aaatggtgga ggacgggacc gtcaccgcag aacagggagc ggagctgatg gccgccctgg
     1381 acgcggtgcc ggcggaggcc gcgctgcgca gctgttatga caaaaagatg ttccgcatcc
     1441 tcgtggacga cgtctcgggg gacaaggtga aaatccagtt tcccgtgggg gcgatcaaaa
     1501 agatcctgaa agtcaccggc aaactgccca tccccgagaa agacctgcag ggggttgacc
     1561 tgtccgccat gatggacgcg gtgtccgagt gcctggagga tgagatcgag ggggatttcg
     1621 tcaacgtgga atccgccgac ggcacgaccg tcagggtatt cgtcgatcaa taaaaaaagt
     1681 aaagagtgga agcatgaaag taaagatcag gacgaaagag ctgcattttt ccatgccgct
     1741 gccggtctcg atgatcggct ttgtcgtaag gctgatcccc gccggggtat tcgataaaat
     1801 ggcggcggac acgccggagc cctatcgctg tcttgtcaca aaggagaccg tcacgatggt
     1861 cttagagagc tgtctggacg ttttgaaaga gaaccgtggc ctggaagccg tgcatgtgga
     1921 agctcacgac ggcacctttg tctccatccg gctctgaaca aaagcgcctg tcctccccgg
     1981 ggtacccccc tcgccgcccg ggtccacccc cttactgcct gtttacagga ttgaaaacac
     2041 cccacaaaaa gccctctccg tctttgcgga gagggctttt tgtggggcaa agggaactgg
     2101 cacgaccgcc tctttcagag gcaaaaagag cagagcgctg cgcgccgcac tttccggctt
     2161 tcttgggaca gcgcgccctt ttccgtctca agcgacggcc gcccggactt ttttcgtgaa
     2221 aggggaacaa aaaaatcagg cgcctgccgc ggcgggatca aaaaagcccg gcaagccgca
     2281 ccacaaattt tttcagggca gagcccccca ccggaaagca aggggtcctg ccctgaacct
     2341 ttttcggaca agccgcgtag tcgagcgttt tttcaggtgc tttcccgttc ccgctttgag
     2401 gcgggcaatt ccgccagctg caggatcagg ctgtccgcaa tttttttcat ctccggggtg
     2461 agctgccggt attttttcat cacctcggag gaagtatctt tccgcggcgg ggtaaaagcc
//
LOCUS       DBMDYA010000440         2514 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8964_length_2514_cov_1.481558, whole genome shotgun sequence.
ACCESSION   DBMDYA010000440 DBMDYA010000000
VERSION     DBMDYA010000440.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8964_length_2514_cov_1.481558"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(163..1332)
                     /locus_tag="ACZPFK_10320"
     CDS             complement(163..1332)
                     /locus_tag="ACZPFK_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1406749.1"
                     /translation="MEKPTSDPGAQQPGKRNNTMSTSKRVVMIVCIVLAALVLVGGIA
                     FAAVYFYLDSQINEGDAGQLTSDVVTTAPEMKEKVVHYLVCGIDYDADDDGRDYGEGQ
                     GMTDVILYVSLDVEKNTMNVLQIPRDTYVGEQAPTGGTCKINAVYSHGADAANRISNL
                     AKLLNDQFQLPVDHYVTIDMAAFKSLLNVLGGIEMYVPWDIVQKDKTTGEETLVAEQG
                     VHKISGETAELILRNRNYSQADYKRLETQQYFYKALFSTFKSFPMSDVIKVMPGFITY
                     VNTDMNVGDMLSFASTVMGMESADIGFVRCPGGPVTRLNTATSRPEACYGINRENLAA
                     LLNDHFRPYVDPVAADSLGLPLIPEEDFTLGQTNEALHSMDALDTEPSANSDEGE"
     gene            complement(1722..2381)
                     /gene="trhA"
                     /locus_tag="ACZPFK_10325"
     CDS             complement(1722..2381)
                     /gene="trhA"
                     /locus_tag="ACZPFK_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1406750.1"
                     /translation="MSTLFRTAQDPISSETHALGAVLSLLGGFVFVFRALGTGASVRD
                     LAGAAVFTLSLIALYAASAIYHYYPGDVNSGGVKRRLRKMDHSMIYVLIAGSYTPFAL
                     KFLPTAKGLRFCLILWGIALAGIVMKLLWINAPRLLTSALYLAMGWSIVFVLRDFASC
                     GAACMALVALGGVSYTVGAVLYAVKRPNFSPEFGFHELFHLFILGGSLLHYLAVFFFV
                     L"
BASE COUNT          607 a          682 c          732 g          493 t
ORIGIN      
        1 aaatcaatcg gcaaaaaaac aaaaaggtaa acgttttttt ggcgcgcgcg gaaaaataaa
       61 agcagaattt agctaaatcc tcccaggagc cgcagggaca caaaactccc ggcccacagg
      121 ccgggaattt ttttaagata aatcggtcgg cggcaaaata gctcattcgc cctcgtcgct
      181 gtttgcagag ggttccgtgt ccagggcgtc catgctgtgc agagcctcgt tggtctggcc
      241 cagggtgaaa tcctcctccg ggatcagggg caagcccagg gaatcggccg ccaccggatc
      301 cacataaggg cggaaatggt cgttcagcag agcggcgaga ttttcccggt tgatgccgta
      361 acaggcctcc ggccgggaag tggcggtgtt cagccgggtg acggggccgc cggggcagcg
      421 gacaaagccg atgtcggcgc tctccatgcc cattacggtg gaggcgaaag agagcatgtc
      481 ccccacgttc atgtctgtgt tgacataggt gatgaagccg ggcatcacct tgatgacgtc
      541 gctcatggga aagctcttaa aagtcgagaa aagggccttg tagaaatact gctgcgtctc
      601 cagccgcttg taatccgcct ggctgtagtt gcggttgcgc aaaatcagct cagcggtctc
      661 gccgctgatt ttgtgcacgc cctgctcagc gaccagggtt tcctccccgg tggtcttatc
      721 cttttgcaca atatcccagg ggacgtacat ctcgataccg cccagcacgt tgagcagcga
      781 tttgaacgca gccatgtcga tggtgacata gtgatccacc ggcagttgga actgatcgtt
      841 gagcagcttt gccaggttgg agatgcggtt ggcggcgtct gccccatggc tgtagaccgc
      901 gttgatcttg caggtgccgc cggtgggcgc ctgctcgccc acataggtgt cgcgggggat
      961 ctgcagcacg ttcatcgtgt ttttttccac gtccagcgag acgtaaagga tgacgtcggt
     1021 catgccctgc ccctcgccat aatcgcggcc gtcgtcgtcc gcgtcatagt cgatcccgca
     1081 gacgagatag tggacgacct tttctttcat ttcgggggcg gtggtcacca cgtcgctggt
     1141 gagctgcccg gcgtccccct cgttgatctg ggaatcaaga taaaaataga cggcggcaaa
     1201 agcgatgcct cccaccagta ccagtgcagc cagcacgata cagacgatca tgacgaccct
     1261 cttggacgtg ctcatcgtat tgttccgttt gccgggctgc tgtgcgcccg ggtctgaagt
     1321 tggtttctcc aaagcaggac ctcctaaaga aaaaagatag gccgagcggc cgaacggccc
     1381 cggggagagg ccgcaaaaaa cgaaatactg gggggcggaa cagcatcaca ttgaatccgc
     1441 aaagccagga ccggcgattt gcagacgttc atcgccctct ccgccgagaa aaagcagacc
     1501 atgccagaac gtaattcctc aattattata ccataaaagc cgcctgaaac cacgaaattt
     1561 gtaaatttaa ggcaaatgct ccacccccgt cggcgcgggg tttttgagaa gggatccgct
     1621 ccggcttttc tctattataa gaaaaagaaa gggccgcagc catcgactgg gcttacggtc
     1681 cgggtaaaat gctttcaatt ttgtaagggc ggcggcaaaa atcagagcac gaagaaaaag
     1741 acggccagat aatgcagcag gctgccgccc aggatgaata gatgaaagag ctcatgaaag
     1801 ccgaattccg gcgaaaaatt gggccgtttg acggcgtaga gcaccgcccc gacggtatag
     1861 gacacgccgc ccagcgccac cagggccatg caggcggcgc cgcagctggc aaagtcgcgc
     1921 aggacaaaga cgatggacca ccccatggcc agatagagcg ccgaggtgag caggcggggg
     1981 gcgttgatcc agagcagctt catcacgata ccggccagcg cgatccccca gaggatcagg
     2041 caaaagcgca gtccctttgc agtgggcagg aatttgagcg caaagggcgt gtagctgccc
     2101 gcgatcagca catagatcat actgtggtcc attttgcgca gccgccgctt gacgccgccc
     2161 gagttgacgt cccccgggta atagtgatag atggcgctgg ccgcgtacag cgcgatcagg
     2221 gagagcgtga ataccgccgc gccggccaaa tcgcggacgg aggccccggt gcccagggcg
     2281 cgaaagacaa agacaaaacc gcccaacagc gatagcactg cccccaaagc gtgggtctcg
     2341 ctgctgatgg ggtcttgtgc ggtgcggaag agagtgctca tgacaattct cctaatttta
     2401 aaatatgatt ttcaatatta tattaactta tttctgaaaa tgtttcaagc gttggcccaa
     2461 taaaataaac gggtaatact ttcagtatag gcagagcgga gcgcaaaata aaca
//
LOCUS       DBMDYA010000441         2512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8968_length_2512_cov_1.242638, whole genome shotgun sequence.
ACCESSION   DBMDYA010000441 DBMDYA010000000
VERSION     DBMDYA010000441.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8968_length_2512_cov_1.242638"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="ACZPFK_10330"
     CDS             <1..1131
                     /locus_tag="ACZPFK_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406751.1"
                     /translation="MQSLGASNITVSLSQKSSDDSGGAVTMEGVSIRMFGPSRPADED
                     LLTDEMIAEYREAFGDKVAAVSITESLGSGAVTQKDTSLNVTITGANLDYLTAESLEV
                     INGRFLNQNDLDGQKKVCVVTDEFAEKLFGEGADPIGKSFEATVNNKSYTFYVVGVTP
                     YDDEGSVAMFGSDVSYPVYIPLTTAKKLNGSAPGYQSFTVVGSSVGDNAQFLSDTESF
                     FASYYTRNQSWTVGASSMESMMSTVTEMISTVQMAIAAIAAISLLVGGIGVMNIMMVS
                     ITERTREIGTRKALGAPGYAIRLQFITESVVICLIGGVIGIVLGVVLGAWGASLLGYA
                     AKPSVLAVAASVGFSMAIGVFFGYYPASKAAKLDPIEALRYE"
     gene            complement(1284..1496)
                     /locus_tag="ACZPFK_10335"
     CDS             complement(1284..1496)
                     /locus_tag="ACZPFK_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR1406752.1"
                     /translation="MNNGTVKWFNDDKGFGFISNDDGSGDVFVHFSAIQSEGFKTLAE
                     GQKVTFDSEVDPKNSSKMRAVNVHIA"
     gene            complement(1874..2350)
                     /locus_tag="ACZPFK_10340"
     CDS             complement(1874..2350)
                     /locus_tag="ACZPFK_10340"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1406753.1"
                     /translation="MNIGLDREFYTQVETVADPDSLLVLCNKYHQLPDGYEPADLTQI
                     SAAHSNGGKTLYLRAEAAAAFEALCEAAAAEGYTILGQSGYRSYAYQQQLYDNYAARD
                     GQAAADTYSARPGFSEHQTGLAMDICNGALSYTEFGQTAEYAWAKENLHRYGFVLH"
BASE COUNT          481 a          771 c          756 g          504 t
ORIGIN      
        1 atgcagagcc tgggcgcgag caacatcacc gtcagcctga gtcagaaaag ctcggacgac
       61 agcggcggcg ccgtcacgat ggagggcgtc agcatccgca tgttcggccc cagccggccc
      121 gccgacgagg acctgctcac cgacgagatg atcgccgagt accgcgaggc gttcggcgac
      181 aaggtggccg ccgtctccat caccgaatcg ctgggcagcg gcgcggtcac ccaaaaggac
      241 accagcctga acgtcactat caccggcgcc aatctggact atctcaccgc cgagagcctg
      301 gaggtcatca acggccgctt tctcaaccag aacgacctgg acggccagaa aaaggtctgc
      361 gtggtgaccg acgagtttgc tgaaaagctc ttcggggagg gagcggaccc catcggcaaa
      421 tcctttgagg cgacggtgaa caacaaatcc tacacctttt acgtcgtggg ggtcaccccc
      481 tatgacgacg agggctccgt ggccatgttc ggcagcgatg tgagctatcc tgtttatatc
      541 cccctcacca ccgccaaaaa gctgaacggc tctgcgccgg gctatcagag ctttaccgtg
      601 gtgggcagtt ccgtggggga caacgcccag tttttatccg ataccgagag ctttttcgcc
      661 agctactaca cccgcaacca gagctggacg gtgggggcct ccagcatgga gagcatgatg
      721 agcaccgtca cggaaatgat ctccaccgtg cagatggcca ttgcggccat cgcggccatc
      781 agcctgttgg tgggcggcat cggcgtgatg aacatcatga tggtctcaat caccgagcgc
      841 acccgcgaga tcggtacccg caaggccctg ggcgcgcccg ggtacgccat ccggctgcag
      901 ttcatcaccg agtcggtggt gatctgcctc atcggcggcg tcatcggcat tgtgctgggc
      961 gtggtgctgg gggcctgggg cgcctccctg ctgggctacg cggccaagcc gtccgtcctg
     1021 gcggtggcgg cctcggtggg cttctccatg gccatcggcg tcttttttgg ctactatccg
     1081 gccagcaagg ccgccaagct ggaccccatc gaagcgctgc gctatgagta gcgccttgaa
     1141 ccctgcatcc gcggccgccc gtcggcccat ccgcagcccc gggaccgcgg ccgcatccgc
     1201 cttataatat agatgaaaaa gagccgcttc tccgtcgatc tgcgacgggg aagcggctct
     1261 tttcaatttg tggtttgccc tgctcaggca atgtgcacgt tgacggcgcg catcttgctg
     1321 gagttcttgg ggtcgacctc ggagtcaaac gtgactttct ggccctcggc gagggttttg
     1381 aagccctcgg actggatggc ggagaagtgc acaaacacgt cgccgctgcc gtcgtcgttg
     1441 gagatgaagc cgaagccctt gtcgtcgttg aaccatttta cagtaccgtt attcataaaa
     1501 ggtacctcct aaaagtagtg ttattccagt gcaaatcatc gtgccgggct ccgggcgaaa
     1561 acgacctgga cagccgtttt ttatagcttt cggtgaaacc tataaaaaaa cggcgccgta
     1621 taaaatcaaa atggaaattg atctcataca gcgcccaatc aattattaca tttagaatac
     1681 ttacttagta taacgctgaa ttttacaaat gtcaaatttt atttttcggc ggccctcatc
     1741 cctgcaggta ggccgcacag tactcgtcaa aggtgatccc cagctcgtga atcttttcgg
     1801 cggtctcggt ccccacatag cggatgtgcc attcctcggt catatacccg gtgatccact
     1861 gggtctcggg cagctagtgg agcacaaagc cgtaccggtg caggttctcc tttgcccagg
     1921 cgtattccgc cgtctgcccg aactcggtat aggacagcgc gccgttgcag atgtccatcg
     1981 ccagcccggt ctggtgctcg gaaaagcccg gccgggcgct gtaggtatcc gccgcggcct
     2041 gcccgtcccg cgccgcatag ttgtcgtaca gctgctgctg ataggcatag ctgcggtagc
     2101 cggattggcc caggatcgtg taaccctccg cggccgcggc ctcgcacagc gcctcaaagg
     2161 cggcggccgc ctccgcccgc agataaaggg tcttgccgcc gttggagtgc gcggccgaga
     2221 tctgggtcag gtcggcgggc tcatagccgt ccggcagctg atggtatttg ttgcagagca
     2281 ccagcaggct gtccgggtcg gccaccgtct ccacctgggt gtaaaactca cggtcaaggc
     2341 cgatgttcac ataggttacc gcctgctgcg gggtgaactc ggggtgcagc gcggcgtaat
     2401 ccgcataacg ggccgcattc tgcgccagat aaccgggcgc ggcggcgaac ggatccgccg
     2461 gcgcgggcgt ttcggtggat ccgttcgccg ccgcgcccgg ttatctggcg ca
//
LOCUS       DBMDYA010000442         2502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_8999_length_2502_cov_1.705539, whole genome shotgun sequence.
ACCESSION   DBMDYA010000442 DBMDYA010000000
VERSION     DBMDYA010000442.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_2502_cov_1.705539"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="ACZPFK_10345"
     CDS             complement(<1..391)
                     /locus_tag="ACZPFK_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR1406754.1"
                     /translation="MNEFDAGLLEGKKRLHFVGIGGSGMFPIVQILHAAGYTITGSDV
                     NEGDIINYERAMGIDVKIPHAAENVEGADLLVYTAAILPGNPEIGRAQELGIPCVERS
                     VMLGYVQRLYAKSICVSGTHGKTTTTGM"
     gene            792..1526
                     /locus_tag="ACZPFK_10350"
     CDS             792..1526
                     /locus_tag="ACZPFK_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406755.1"
                     /translation="MEAAVEMLELLCRAAEGDGAAEAKLEASCAGANGFSALPSRRVR
                     DAVQAALLARRPQAVYLPARAGCLGRFGLPGVRFELPLQALPAQMLLRWWGLIRACGG
                     RPARVVRGFGFSPSFQRDLERLDELYFAPPPASLRELKQQLRQPLPQREEELFAAFAA
                     VDPGFEPRCALLRQLRASGEPYTPEMLAIRPASLRALGLSGGSIAPVRELLLDAVIRA
                     PELNRYETLARMAVELGPLARGRKGF"
     gene            1523..>2502
                     /locus_tag="ACZPFK_10355"
     CDS             1523..>2502
                     /locus_tag="ACZPFK_10355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1406756.1"
                     /translation="MSTKNREMPDCAHVDVADGFRAASALLVLWFHFWQQNWLRPEIA
                     LPFLAPVGLASINLEWLPRSGYLFVDALLMLSGFCLFLPVARAVAEERPDAGRIDARD
                     FYRRRVARILPSYLFCLAVILIFCVLPDWPYAAAKDFWTDLLAHLTFTHSFFPASYLG
                     TQLNGALWTLSIEAQFYLLFPLLARWFARAPLRAWAVMNGISLASVAALALLRPDLGM
                     WLNQLPLQLCTYADGMLAALCFVRLARRPRRGRWEGLVFSALALACIGGVAGLVKRQG
                     GAPDLQLGQLLLRLPLSALFAALLVCLALSARPLRWLFSNRLMRFLSKIY"
BASE COUNT          343 a          783 c          857 g          519 t
ORIGIN      
        1 tcatgccggt ggtggtggtc ttgccgtggg tgccggagac gcagatcgac ttggcataca
       61 gccgctgcac atagcccagc atcacgctgc gctccacaca ggggatgccc agctcctgcg
      121 cgcgcccgat ctccgggttg cccggcagaa tggcggcggt atacaccagc aggtcggctc
      181 cctccacatt ttcagcggcg tgggggatct tcacgtcgat gcccatcgcc cgctcgtaat
      241 tgatgatgtc gccctcgttg acgtcgctgc cggtgatggt atacccggcc gcgtgcagga
      301 tctgcacgat aggaaacatg ccgctgcccc cgatgcccac gaaatgcagt ctctttttac
      361 cctctaacaa tccggcgtca aactcgttca atctagtctc cccgcttcct attttcactc
      421 tgccaccagt ctatggtggg catgcccggc gcgttccaaa caaaattgcg gcccctgcgc
      481 gggcaaaaag gcgttcaaag cgcaaaaaat cctcgttacc ggcccatcgg cgggacaggc
      541 tctccccgct caaaaccatg gttaatatta tagtattttt tgtcgccgaa agaaacacct
      601 ttgccgaaat ttcgcagtct gttataatag gagaacagat ggggcgtctg gcggccgccg
      661 cctgtaaggg gcggggtgcg gatgagggcc aaagctttga ccgcggccct gcggcggtgc
      721 ggcgcagagc ggcgcgatgg gagcctttct cgccccaggc tgcggaataa aaggatgcgg
      781 gaggtgcgcc gatggaagct gcggtggaga tgctggaact gctgtgccgc gcggcggagg
      841 gggacggtgc ggccgaggca aagctggaag cgtcctgtgc gggtgcgaac ggtttttcgg
      901 ccctgccgtc ccgcagggtg cgggatgcgg tgcaggcggc gctgctcgcc cgccggccgc
      961 aggcggtcta tttaccggcg cgcgcgggct gcctcggccg cttcggcctg ccgggcgtcc
     1021 gctttgagct gccgctgcag gccctgcccg cgcagatgct gctgcgctgg tgggggctga
     1081 tacgggcttg cggcggccgc ccggcgcggg tggtgcgggg ctttgggttc tcaccgtcgt
     1141 ttcagcggga cctggagcgg ctggacgagc tgtattttgc ccctccgccg gcttcgctgc
     1201 gggagctgaa gcagcagctg cggcagcccc tgccgcagcg ggaggaggag ttgtttgcgg
     1261 cctttgccgc agtggacccc ggatttgagc cgcgctgtgc gctgctgcgg cagctgcggg
     1321 cgagcgggga gccctacacg ccggaaatgc tggccatccg gcccgcctcg ctgcgggccc
     1381 tgggcctgtc cggcggcagc atcgccccgg tgcgggaatt gctgctggac gcggtgatcc
     1441 gcgccccgga gctcaatcga tatgagacat tggcgcggat ggcggtggag ctgggaccgc
     1501 tggcgcgggg gaggaaaggc ttttgagcac gaaaaacaga gaaatgcccg actgcgcgca
     1561 tgtggatgtg gcggacggtt ttcgcgccgc cagcgccctg ctggtgctct ggttccactt
     1621 ttggcagcag aactggcttc ggccggagat cgcgctcccc tttctcgccc cggtggggct
     1681 cgcctcgatc aatctggaat ggctgccgcg cagcggctat ttgtttgtgg acgcgctgct
     1741 gatgctcagc gggttttgcc tctttttgcc ggtcgcccgc gccgtggccg aggaacggcc
     1801 ggacgcgggg cgcatcgatg cgcgcgattt ttaccgcaga cgggtggcgc gcatcctgcc
     1861 cagctacctg ttctgtcttg ccgtgatcct gattttttgc gttctgccgg attggcccta
     1921 tgcggcggca aaagattttt ggaccgatct gttggcccat ctcaccttta cccattcttt
     1981 ttttccggcc tcctacctgg gcacccagct caacggcgcg ctgtggacgc tgagcatcga
     2041 ggcgcagttt tacctcctct ttccgctgct ggcgcgctgg tttgcccgcg cgccgctgcg
     2101 cgcctgggcg gtgatgaacg ggatctcgct ggcgtcggtg gcggcgctgg ccctgctgcg
     2161 gcccgacctg gggatgtggc tcaaccagct gcccttgcag ctttgcacct acgccgacgg
     2221 catgctggcg gcgctctgct ttgtgcggct ggcaaggcgg ccgcggcgcg gacggtggga
     2281 ggggctcgtc ttttccgcgc ttgccctggc ctgtattggg ggcgtcgcag ggctggtaaa
     2341 gcgccaggga ggcgcaccgg acctgcagct tggccagctg ctgctgcgcc tgccgctctc
     2401 agcgctgttc gcggcgctgc tggtctgcct ggccctgtcc gcgcgcccgc tgcgctggct
     2461 gttctccaac cgtctgatgc gctttttgtc gaagatctat tt
//
LOCUS       DBMDYA010000443         2499 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9012_length_2499_cov_1.245621, whole genome shotgun sequence.
ACCESSION   DBMDYA010000443 DBMDYA010000000
VERSION     DBMDYA010000443.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_2499_cov_1.245621"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            323..1507
                     /locus_tag="ACZPFK_10360"
     CDS             323..1507
                     /locus_tag="ACZPFK_10360"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR1406757.1"
                     /translation="MSKEIVLVGACRTAIGKMGGALSNVPAEQLGAIVMKEAMNRAGI
                     APEQIDEVLFGNVLTAAMGQNPARQAAVHAGIPYSVPALTINNVCGSGLKTVNMAAAM
                     IEAGEADVIMAGGMENMSMAPYALTKARFGYRMNNSTMVDTMVNDALWDAFNDYHMGI
                     TAENVAEEYEITREMQDEFAAWSQQKCEAARKAGKFDDEIVGVPVKVKKETVIFNKDE
                     GPRDGVTAEGIAKLRPAFRADGTVTAANASGINDGAAAIIVMSAEKAKELGVKPMARW
                     VGGASGGVDPSVMGVGPATSTKKLFDKTGLRINQMDLIEANEAFAAQSLAVGKLLNWD
                     AEKVNVNGGAIALGHPVGASGCRILVTLLYEMQKRGSKYGLATLCVGGGMGVSTIVEG
                     IK"
     gene            1554..2330
                     /locus_tag="ACZPFK_10365"
     CDS             1554..2330
                     /locus_tag="ACZPFK_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR1406758.1"
                     /translation="MSFVKVEQKEGYAILTIDREKALNALNSEVLGDLEAAVDALDLD
                     AVRALIITGAGSKSFVAGADIGEMSGLTKAEGEAFGKKGNDVFRKIESLPIPVIAAVN
                     GFALGGGCELSLACDIRIASENARFGQPEVGLGITPGFGGTQRLARTIGVGKAKELLY
                     TCAQVKAPEALALGLVNAVYPIDELLPAAEKMAAAIAANAPIAVRATKKAVNDGLQVG
                     IDEAIVIEEKLFGSCFESADQKEGMAAFLERRPHAPYQNK"
BASE COUNT          534 a          810 c          776 g          379 t
ORIGIN      
        1 tgtccatctc cccttaacag aagcggggcg caaacctggt aagataaaag tgccgcccga
       61 ccgggtgcgc gcgcaggtgc aagaggggta ggggacgtcg cgtcaaatcc gacaacgaag
      121 tttgttaaat tttcgcaaaa ttggacagtt caccaaaact gacacaacct gtttaatttt
      181 taacaatccc ccttgaaagc cccaccccat tgtgatatat ttaacatgta cccggagggg
      241 ggaacgcccc tctcccaccc gagagaaaca gagacagggc agaccgaaaa aaggcaacat
      301 taaaaaatgg agggaaccaa ttatgtctaa ggaaatcgta ctcgtgggcg cctgccgcac
      361 cgccatcggc aagatgggcg gcgcgctgtc caacgtcccc gccgagcagc tcggcgcgat
      421 cgtgatgaaa gaggccatga accgggccgg catcgccccg gagcagattg acgaagtgct
      481 tttcggcaac gtgctcaccg cggccatggg ccagaacccc gcccgccagg ccgccgtcca
      541 cgccggcatc ccctacagcg tgcccgccct caccatcaac aatgtctgcg gcagcggcct
      601 gaagaccgtc aacatggcgg ccgccatgat cgaggccggc gaggccgacg tcatcatggc
      661 cggcggcatg gagaacatgt ccatggctcc ctacgcgctg accaaggccc gcttcggcta
      721 ccgcatgaac aattccacca tggtcgacac catggtcaac gacgccctgt gggacgcttt
      781 caatgattat cacatgggca tcaccgccga gaacgtggcc gaggagtatg agatcacccg
      841 cgagatgcag gatgagttcg ccgcctggag ccagcagaag tgcgaggccg cccgcaaggc
      901 cggcaagttc gacgacgaga tcgtcggcgt gcccgtgaag gtcaaaaaag agaccgtcat
      961 cttcaacaag gacgagggcc cccgcgacgg cgtcaccgcc gagggcatcg ccaagctgcg
     1021 cccggcgttc cgcgccgacg gcaccgtgac cgcggccaac gcctccggca tcaacgacgg
     1081 cgccgccgcc atcatcgtca tgagcgctga aaaagccaag gagctgggcg tcaagccgat
     1141 ggcccgctgg gtcggcggcg cttccggcgg cgtggatccc tccgtcatgg gcgtgggccc
     1201 cgccacctcc accaaaaagc tgttcgacaa gaccggcctg cgcatcaacc agatggatct
     1261 gatcgaggcc aacgaggcct ttgccgccca gagcctggct gtgggcaagc tgctcaactg
     1321 ggatgccgaa aaggtcaacg tcaacggcgg tgccatcgcg ctgggccacc ctgtgggcgc
     1381 ttccggctgc cgcatcctgg tcaccctgct ctatgagatg cagaagcgcg gctccaagta
     1441 cggcctggcc accctgtgcg tcggcggcgg catgggcgtc tccaccatcg tcgagggcat
     1501 caaataagaa cgccccccga ggcgccacct gaaacgagaa ggagaatcgt aacatgagct
     1561 ttgtgaaagt ggaacagaaa gagggctacg ctatcctcac catcgaccgc gaaaaggcgc
     1621 tcaacgccct caacagcgag gtgctgggcg acctggaggc cgctgtggac gcgctggatc
     1681 tggacgccgt gcgcgccctg atcatcaccg gcgcgggcag caagagcttt gtcgcgggcg
     1741 ccgacatcgg cgagatgagc ggcctgacca aggccgaggg cgaggctttc ggcaaaaagg
     1801 gcaacgacgt cttccgcaag atcgagagcc tgcccatccc ggtcatcgcc gcagtgaacg
     1861 gctttgcgct gggcggcggc tgcgagctgt ccctggcctg cgatatccgc atcgccagcg
     1921 agaacgcccg cttcggccag cccgaggtcg gtctcggcat caccccgggc ttcggcggca
     1981 cccagcgcct ggcgcgcacc atcggtgtgg gcaaggccaa agagctgctt tacacctgcg
     2041 cccaggtcaa ggcgcccgag gcgctggctt tgggtctcgt gaacgccgtc taccccatcg
     2101 acgagctgct gcccgccgcc gagaagatgg ccgccgccat cgccgccaac gcgcccatcg
     2161 cggtgcgcgc caccaaaaag gcggtcaacg acgggttgca ggtgggcatc gacgaggcca
     2221 tcgtcatcga agagaagctg ttcggcagct gctttgagag cgccgatcag aaagagggca
     2281 tggccgcgtt cctggagcgc cgcccgcacg ccccttacca gaacaaatga gatcggaccg
     2341 gcgtgggcgg gcaactgccc gcgctggtcg cgtttcaccc ggtaaagacc acacaggttt
     2401 tacataagtt ttgcgttcca gtgagaggga atgccggcgc gggcggctgc gcccggccgc
     2461 cggcactgcc cgggatcagg atcattttaa aaattagga
//
LOCUS       DBMDYA010000444         2499 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9013_length_2499_cov_1.221018, whole genome shotgun sequence.
ACCESSION   DBMDYA010000444 DBMDYA010000000
VERSION     DBMDYA010000444.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_2499_cov_1.221018"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="ACZPFK_10370"
     CDS             complement(<1..2033)
                     /locus_tag="ACZPFK_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1406759.1"
                     /translation="MKKRWACLLLAASMLLATGCGAGADASGGNSGAGGTQARGRFVE
                     SDVTPEGSGAGLPSGLYTHPDGSVDYLWYDLTEGEGKNSLHHHRSTDGGVTWEEQGAS
                     WQQEFEELRAVLALSDGGLIVISSAEEKGVSLWKITPGGQRTQLELPELEERLGTGGM
                     VIDARLLSDDRLYLQFMGGKMESGSSDGAGVSPAGAAEVDGGESAPSAVFDAGGIADA
                     VFDLSTGALLTDLGHENVYQVAAAGERFVVSDYEGNLSAYNMKDGAPDSSFSGSLGGA
                     LETTLGAAADDEGNFYYINTQGIYRLVQGGSLSEALMEGSRYSFGSPTYGINAFARTA
                     EGDFLAALSLPDGGSRLMRYVYDPEALTRPDKELRVYSLTDSPTARAAITAFQQSRQD
                     VQVNYEVALEEGASAQQREDALRTLNTELVAGQGPDVLILDGLPLESYIDKGALADLD
                     GLLDTDGVIPQLLEPFRTENGIFALPTRFGIPALFTTPERSSGWNTLTQLAEAVAAGN
                     AKPAAGDPDSAFSSIPEEQRPVLSFQSWQEFFDLFYSANASALAGRQKGVEETNLRTF
                     LQALKMISDRCGLMEESGGWGGGLAISSSGGGGFTNVSGGVMRYGMKQALAGCGEVVD
                     LMLIQYAAGVEDGAAMGLLPGLADGAWVPHVLAGISETSGQKELAADFLEAMLS"
     gene            2246..>2499
                     /locus_tag="ACZPFK_10375"
     CDS             2246..>2499
                     /locus_tag="ACZPFK_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1406760.1"
                     /translation="MRILMIEDDRELCEACAPQLRAAGFEADFCHDGGEALYYLRQPL
                     YDLCVLDRMLPGADGLSLLAAARAEGVRTPVLMLTAMGRVG"
BASE COUNT          428 a          887 c          747 g          437 t
ORIGIN      
        1 gagagcatgg cctcgagaaa atccgccgcc agctcttttt gcccgctggt ttcgctgatc
       61 cccgccagga catgcggcac ccaggcgcca tccgccaggc cgggcagcag ccccatggcg
      121 gccccgtcct cgacgcccgc ggcgtactgg ataagcatca ggtccaccac ctcgccacag
      181 cccgccaggg cctgcttcat gccgtagcgc atcacgccgc cggacacatt ggtaaaaccg
      241 ccgccaccgc tgctggagat ggcgagcccg ccgccccagc cgccgctctc ctccatgagg
      301 ccgcagcggt ctgagatcat cttcagcgcc tgtaggaaag tgcgcagatt ggtctcctcc
      361 acccccttct gccgcccggc cagggccgag gcgttggcgc tgtaaaacag gtcgaaaaac
      421 tcctgccagc tttgaaaaga gagcacgggc cgctgctcct ccggtattga cgaaaaagcc
      481 gagtccgggt cgccggccgc cggttttgcg ttgcccgccg ccaccgcctc cgccagctgg
      541 gtgagggtgt tccagcccga gctgcgctcc ggtgtggtga aaagcgccgg gatgccgaac
      601 cgggtgggca gggcaaatat cccgttttcc gtcctaaagg gctccagcag ctgcgggatc
      661 acgccgtccg tgtccagcag gccgtccagg tccgccaaag cgcccttgtc gatataggat
      721 tcaaggggca gaccgtccag gatcagcacg tccggcccct gcccggccac cagctcggtg
      781 ttcagggtgc gcagcgcgtc ctcgcgctgt tgggcgctcg cgccctcctc cagcgcgacc
      841 tcatagttca cctgcacgtc ttgacgcgac tgctgaaaag ccgtgatcgc cgcccgcgcg
      901 gtgggggaat cggtgaggga gtagacccga agttccttgt ccggccgggt gagcgcctcg
      961 ggatcataga catagcgcat cagccgactg ccgccgtcgg gcagggacag ggccgccaaa
     1021 aaatccccct ctgcggtgcg cgcaaatgcg ttgatgccat aggtgggcga gccaaagctg
     1081 tagcggctgc cctccatcag ggcctcggac aagctgccgc cctgcacgag ccggtaaatg
     1141 ccctgggtgt tgatgtaata aaagttgccc tcgtcatccg ccgccgcgcc caaagtcgtc
     1201 tccagggcgc cgcccaggct gccgctgaag ctgctgtccg gcgcgccgtc tttcatattg
     1261 taagcgctca ggttgccctc atagtccgag accacaaacc gctcccccgc cgcggccacc
     1321 tgatagacgt tctcatgccc caaatcagtg agcagcgcgc cggtggagag atcgaacacc
     1381 gcgtcggcga tgccgcccgc gtcaaagacg gcggaagggg cgctctcgcc cccgtccacc
     1441 tcagcggccc ccgccggtga aacgcccgcg ccgtctgagc ttccgctttc catctttccg
     1501 cccataaact ggagatagag acggtcgtcg gacaacagcc gggcatctat gaccatgccc
     1561 cccgtgccca gcctctcttc cagctccggc agctccagct gggtcctctg gccgcccggc
     1621 gtgattttcc acagagaaac gcccttttct tcggcgctgc tgatcacaat caagccgccg
     1681 tccgacagcg ccagcaccgc ccgcagctcc tcaaactcct gctgccacga ggcgccctgc
     1741 tcctcccagg tgacaccgcc gtccgtcgac cggtggtggt gcaagctatt tttgccctcc
     1801 ccctccgtca ggtcatacca gagataatcg acgctgccgt ccggatgggt gtagagaccg
     1861 gagggcaggc cggcgccgct gccctccggc gtgacgtcgc tctctacaaa acggcctctg
     1921 gcctgtgtgc cccccgcgcc gctgttgccg ccggaggcat ccgcccccgc gccgcagccg
     1981 gtggccagca gcatcgacgc cgccagcagc aggcaggccc atcgtttttt catcttcaag
     2041 atgatcgcct ctctctcttg tccttgcggg gccgcgcccc gtccacggct caaactatac
     2101 cgccgcacac cttgaaaaat ccttaaaaaa cagaggtgaa agccattttc acagtttttt
     2161 catggatcgg cgcgcccttt tcaggacgtt ttaaggtaat ataaggtaga ctggccccaa
     2221 cggacaaaat aaaggagcat ctcagatgag gatcttgatg atcgaggacg accgggagct
     2281 gtgcgaggcc tgcgccccgc agctgcgggc cgcgggcttt gaggccgact tttgccatga
     2341 cggcggggag gcgctttatt acctgcgcca gcccctctat gacctgtgcg tcctggaccg
     2401 catgctgccc ggcgccgacg ggctgagcct gctggccgcc gcccgcgccg agggggtgcg
     2461 cacgccggtg ctgatgctca ccgccatggg gcgggtggg
//
LOCUS       DBMDYA010000445         2489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9056_length_2489_cov_1.453936, whole genome shotgun sequence.
ACCESSION   DBMDYA010000445 DBMDYA010000000
VERSION     DBMDYA010000445.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9056_length_2489_cov_1.453936"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="ACZPFK_10380"
     CDS             <1..451
                     /locus_tag="ACZPFK_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406761.1"
                     /translation="MYVADDGEGAGGGGGIVEVNGYDTAVVTLVTDDVGEGEKNLYWT
                     PARGAASFAENWSQKTADDLPEGGGHQYYTEGRAKAAAGTVFDEKFAQKTTDALAEGA
                     QHQYYTAGRATENFKANIAGTSVLTLKDVDQLVTKQKSYIIDAGRLG"
     gene            494..1849
                     /locus_tag="ACZPFK_10385"
     CDS             494..1849
                     /locus_tag="ACZPFK_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406762.1"
                     /translation="MFEVTASGCEYFGSPDAGDAMGTLAAGDVYRALAASATEQGGCT
                     WLQFEKDGRTCCVALRKDRVRLYTRAATADSAAGGASDFSAESAATSSAPRGAEGGGS
                     VGGVSDFSAESASTSSAPRGAEGGGSAGEVLDFSAESASTLSAPRGAESGGALSMRMQ
                     QKRATAAVWENSSLILLEGEIGLELDADNNTIGQKIGNGLDVWSALPYSAGGGHVIQD
                     SAGTELPKEGVLQFAGMEVTDDPAGGKTVVHGLGVKLADRAPVPGVPLHAVLKPVGIA
                     PPVPPEGRADGTTITVDNETGVWSVAQAPKADRLATTRKIGEASFDGTADVTLAQMGT
                     LPALSYSDKEIQTGETWCDGRPIYVRTIPVIIPNNTNTNFSTGATNVKECWVSAPSFF
                     QNENIFLPLPYTHVQGFNYQVMVTCQIENGTLSVSLGTTGSSFGGKNGYVTVKYTKTT
                     D"
     gene            1887..2444
                     /locus_tag="ACZPFK_10390"
     CDS             1887..2444
                     /locus_tag="ACZPFK_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406763.1"
                     /translation="MSENVTAYAPYDSAGNRMPLACPVEYLYDENGYGAGERRFASGE
                     QGAKTDTLATGAVYSTKEVKTGETWIDGRPIYRKVFTFELPEISSGNTSARFNLDTDI
                     DLGAIIDLTARFNQNGKTNYGVPMGADFVNPAFSTFVFWSSAVAKRLLIDYGSGVSKQ
                     PALAIIKYTKSTDQATAEQGANENA"
BASE COUNT          576 a          641 c          804 g          468 t
ORIGIN      
        1 gatgtacgtc gccgacgatg gcgagggcgc agggggcggt ggcggcatcg tggaggtcaa
       61 cggctacgac accgccgttg tgacgctggt cactgacgat gtgggcgagg gtgaaaagaa
      121 cctctattgg acgcccgccc ggggtgcggc cagctttgcg gaaaactgga gccaaaaaac
      181 cgccgatgat ctgccggagg gcggcggcca ccagtattac accgaggggc gggcaaaagc
      241 ggcggcgggc actgtttttg atgagaagtt cgcccagaaa acgaccgacg ccctggcgga
      301 gggcgcgcag caccagtatt acaccgccgg gcgcgccacg gaaaatttta aagcgaacat
      361 tgccggcacc tctgtgctga ccctcaagga tgtggatcag cttgtgacaa agcagaagtc
      421 ttatatcatc gacgccggca gacttggata ggagggatgg catatcgaat ggatcgcgct
      481 cagcgcggac tcggtgtttg aagtgacggc gtccggctgc gagtacttcg gctcgccgga
      541 tgcgggcgac gcgatgggaa cgctggcggc cggggacgtc taccgggctc ttgccgcctc
      601 cgccacagag cagggaggct gtacctggct tcaatttgaa aaggatgggc ggacctgctg
      661 cgtcgctttg cgcaaggacc gcgtccggct gtacacccgc gccgccacag ccgacagcgc
      721 cgcgggtggg gcttcggatt tctcggccga gagcgcagcc acttcgtccg cgccgcgggg
      781 cgcggagggc ggcggctctg tgggtggggt ttcggatttc tcggccgaga gcgcaagcac
      841 ttcgtccgcg ccgcggggtg cggagggcgg cggctctgcg ggcgaggtct tggatttctc
      901 ggccgagagc gcaagcacgc tttccgcgcc tcgtggcgcg gaaagcggcg gcgcgctctc
      961 catgcgcatg cagcaaaaac gcgccaccgc cgccgtgtgg gaaaattctt cgctaatcct
     1021 gctggaaggc gagatcgggc tggaactcga cgcagacaac aacacgatcg ggcaaaagat
     1081 cggcaacggc ttggacgtgt ggagcgcgct gccctactcc gccggcggcg ggcatgtaat
     1141 ccaggactcg gcggggaccg aattgcccaa agagggagtt ctgcaatttg cgggcatgga
     1201 ggtgaccgac gacccggcgg gcggcaagac ggtggtacat ggcctggggg tgaaactggc
     1261 ggaccgcgcg ccggtgccgg gggtgcctct gcacgcggtg ttaaagccgg tgggcatcgc
     1321 cccgcctgtg ccccccgagg gccgcgccga cggcacgaca atcaccgtgg ataatgagac
     1381 cggggtgtgg agcgtggccc aggcgccgaa agcggacagg ctggccacga cgagaaagat
     1441 cggcgaggcc agctttgacg ggacggcgga tgtcacgctg gcgcagatgg gcactctccc
     1501 tgctttgtct tattccgata aagagataca gactggtgaa acatggtgtg atggcaggcc
     1561 catttacgtt cgaacaattc cagttattat cccgaataat acaaacacta acttttcaac
     1621 tggcgcaacg aacgtaaaag aatgttgggt atctgcacct tctttttttc aaaacgaaaa
     1681 tatttttttg ccgttgcctt atactcatgt acaaggtttt aattatcaag tgatggtaac
     1741 ttgccaaatt gaaaatggaa cattatctgt tagcttggga acaacaggga gttcgtttgg
     1801 cggcaaaaat ggatatgtaa cagtaaagta cacaaagact actgattaat tggtggaatg
     1861 acaagataca atggaggcga acaacaatgt ctgaaaacgt gacggcctat gcaccgtatg
     1921 acagcgcggg caaccggatg ccgctggcct gcccggtgga atatctctac gatgagaacg
     1981 ggtacggggc cggggagcgg cggtttgcct cgggggagca gggggcaaaa acagacacac
     2041 tggccaccgg agcggtctac agcacaaagg aggttaagac gggagaaaca tggatcgatg
     2101 ggcgaccgat ttatcgcaag gtatttactt ttgaattgcc agaaatttca agtggaaaca
     2161 cctctgcccg atttaattta gatacggata tagacctggg ggcgatcatt gatctcaccg
     2221 cacgattcaa tcaaaacgga aaaacaaatt atggggttcc aatgggtgcc gattttgtta
     2281 atccggcatt cagcactttt gttttttggt cctcggcggt ggctaaaagg ctgctaattg
     2341 actacggttc tggcgtgtcc aagcaacccg cgctggccat catcaaatat accaaaagca
     2401 ccgaccaagc gacagcagag caaggggcga atgaaaatgc ctgaagagct gacgaccaaa
     2461 caatttattg atatggagac cggcaagcc
//
LOCUS       DBMDYA010000446         2487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9063_length_2487_cov_1.521375, whole genome shotgun sequence.
ACCESSION   DBMDYA010000446 DBMDYA010000000
VERSION     DBMDYA010000446.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9063_length_2487_cov_1.521375"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(15..2468)
                     /locus_tag="ACZPFK_10395"
     CDS             complement(15..2468)
                     /locus_tag="ACZPFK_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1406764.1"
                     /translation="MQVRGARVHNLKNIDVDIPLNKIVGVTGVSGSGKSSLALGVLYA
                     EGSRRYLDALSTYTRRRMTQAAKAEVDEVLYVPASLALHQRPGVPGIRSTFGTGTELL
                     NSLRLMFSRLASHRCPKGHYLPPTLAVAAGHELTCPQCGETFFPPSAEELAFNSQGAC
                     RTCGGTGIVRTVDRASLVPDESLTIDEGAVAPWNSLMWSLMVDVCREMGVRTDVPFRE
                     LTDQEKDIVYNGPAVKKHVIYHSKTTNQGGELDFTYYNAVYTVENALAKVKDEKGMKR
                     VEKFLKEDVCPDCGGTRLSAAARAPKLRGVSLDEACRFTLTELVGWVEGVPASLPAEM
                     RPMAQSICESFQIVARRLGYLTLDRAASTLSTGERQRMQLARAVRNRTTGVLYVLDEP
                     SIGLHPSNIVGLTAVMNDLVADGNSVLLVDHDTQILSAADWLIEMGPQAGADGGRVVA
                     QGTVAELCENGASLIGPFLSGKAQVRVRRSTRPQELFAAGRIRLSTAAIHTVKPLRVE
                     LPKGKLTAVTGVSGSGKTTLILESLIPGLEAAVAGAPLPAHVLSIEAEGISRVKLIDA
                     TPIGINVRSTVATYANVHDELRKLCAKTPDAKRLGFKAGDFSYNTGKLRCPVCDGTGV
                     ISLDVQFLPDVDIPCPDCRGSRYAKDAGRVRLPGPSGQAHSLPELMAMDINTALEACA
                     EMKIVRQRLQVLQSLGLGYLTLGEATPSLSGGEAQRLKLASEMGRGQSDSVFVFDEPT
                     IGLHPLDVRTLLGVFQTLIESGATVIVIEHDLDVIRNADYIVDMGPGGGAEGGRIVAA
                     GTPAEIARNPASITGGYLR"
BASE COUNT          369 a          846 c          871 g          401 t
ORIGIN      
        1 cgcacgccgc gcgtttaccg cagatagccc cccgtgatgc tggccgggtt gcgggcgatc
       61 tcggcggggg tgccggccgc gacgatccgg ccgccctccg cgcctccgcc ggggcccatg
      121 tccacgatgt aatccgcgtt ccggatcacg tccaggtcat gctcgatcac gatgacggtg
      181 gcgccgctct caatgagggt ctgaaagacg cccagcagcg tgcgcacatc cagcgggtgc
      241 aggccgatgg tgggctcgtc gaagacaaag acagagtccg actggccgcg gcccatctcg
      301 ctggccagct tcagccgctg cgcctcgccg cccgagaggc tgggggtcgc ctcgcccagg
      361 gtgaggtagc ccaggcccag gctctgcagc acctgcaggc gctgccggac gatcttcatc
      421 tcggcacagg cctccagggc ggtgttgatg tccatcgcca tcagctccgg cagcgagtgg
      481 gcctggccgg agggcccggg cagccgcacc cggcccgcgt ccttggcgta acgggagccg
      541 cggcagtccg ggcaggggat gtccacatcc ggcaaaaact gcacgtccag gctgatgacc
      601 cccgtgccgt cgcacacggg gcagcgcagc ttgcccgtgt tgtaggaaaa gtcgccggcc
      661 ttgaagccca ggcgcttggc gtccggcgtt tttgcgcaga gcttgcgcag ctcgtcatga
      721 acgtttgcat aggtcgccac ggtggagcgg acgttgatgc cgatgggggt ggcgtcgatc
      781 agcttcaccc ggctgatgcc ctccgcctcg atcgagagga cgtgcgccgg cagcggcgcg
      841 ccggccacag cggcctccag cccggggatc aggctctcca ggatcagcgt cgtcttgccg
      901 gagccggaca cgccggtgac ggcggtcagc ttgcccttgg gcagctccac ccgcaggggc
      961 ttcacggtgt ggatggccgc tgtggacagg cggatgcggc cggcggcgaa cagctcctgc
     1021 gggcgggtgc tccgccgcac ccgcacctgc gccttgccgg agaggaacgg cccgatcagg
     1081 gaggcgccgt tttcacacag ctccgccacc gtgccctgcg ccacgacgcg tccgccgtcc
     1141 gcaccggcct gggggcccat ctcgatgagc cagtccgccg ccgataagat ctgggtgtcg
     1201 tggtccacca gcagcaccga gttgccgtcc gcgaccagat cgttcatgac cgcggtcagc
     1261 cccacgatgt tggagggatg cagcccgatg gagggctcgt ccagcacgta cagcacgccg
     1321 gtggtgcggt tgcgcaccgc gcgggcgagc tgcatgcgct ggcgctcccc ggtggagagg
     1381 gtggaggcgg cgcggtcgag ggtgagatag cccagccgcc gcgccacgat ctgaaacgac
     1441 tcgcagatgc tctgcgccat ggggcgcatc tccgcgggca gcgaggcggg caccccctcc
     1501 acccacccca ccagctcggt cagggtgaac cggcaggcct cgtccaggct gacgccgcgc
     1561 agcttgggcg cccgggcggc cgcggaaaga cgggtgccgc cgcagtcggg gcagacgtcc
     1621 tccttgagaa acttttccac ccgcttcatg cccttttcgt ccttgacctt cgccagggca
     1681 ttttccaccg tgtagacggc gttataatag gtgaaatcca gctcaccgcc ctggttggtg
     1741 gtcttggagt ggtagatgac atgctttttg acggcggggc cgttgtagac gatgtccttt
     1801 tcctggtcgg tcagctcgcg gaaagggacg tccgtgcgca cccccatctc gcggcagacg
     1861 tccaccatca gcgaccacat cagcgagttc cagggggcca ccgccccctc gtcgatggtg
     1921 agggattcgt cgggcaccag cgaggcgcgg tcgacggtgc gcacgatgcc ggtgccgccg
     1981 caggtgcggc aggcgccctg gctgttaaag gccagctcct cggcgctggg cgggaaaaag
     2041 gtctccccgc actgcgggca ggtcagctca tgccccgccg ccaccgccag ggtgggaggc
     2101 aggtagtgcc cctttgggca gcggtggctg gccagccgcg agaacatcag ccgcaggctg
     2161 ttcagcagct cggtcccggt gccgaacgtg ctgcggatgc cgggcacgcc ggggcgctgg
     2221 tgcagggcca gcgaggcggg cacatacagc acctcgtcca cctccgcttt cgcggcctgg
     2281 gtcatgcgcc tgcgggtgta ggtagagagg gcgtccaggt accgccggga gccctcggcg
     2341 tacaggaccc ccagcgccag cgacgatttg cccgagccgg acacgccggt gacgccgacg
     2401 atcttgttca gcgggatgtc gacgtcgatg tttttcaggt tgtgcaccct tgcgccccgg
     2461 acctggatcc tttttggggc ctgggat
//
LOCUS       DBMDYA010000447         2484 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9077_length_2484_cov_1.657574, whole genome shotgun sequence.
ACCESSION   DBMDYA010000447 DBMDYA010000000
VERSION     DBMDYA010000447.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9077_length_2484_cov_1.657574"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(122..952)
                     /locus_tag="ACZPFK_10400"
     CDS             complement(122..952)
                     /locus_tag="ACZPFK_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406765.1"
                     /translation="MRKLKWEQFGIMNMNYLFYTLDYFLDAARGMGVKNINLWTANPH
                     VHLEDATDEDFKKIGEKIASRGLHCFCVCPEQVVYPFNIAAEDDLIRDRSISSMKRAA
                     ELCRILQAPLLHVCPGWGFYDQPDGLKVAWELARSAIAEIAAFAAEQGVTLALEPLQI
                     VESNLVGDIPSAKRMLDELGLPNVKLLVDTCHMAVKGERLEDYFHTFGKDIVHIHLNE
                     SGQVPWGYGNLPLKEYISQMEEQDFDGTITLEVCSRKHYVDPNPSVGRGVETVKAAIR
                     "
     gene            complement(981..1805)
                     /locus_tag="ACZPFK_10405"
     CDS             complement(981..1805)
                     /locus_tag="ACZPFK_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1406766.1"
                     /translation="MKIALLSLLFRRYHLEKVFEVASEQGYDGVEIWGGRPQAYPYDM
                     DAARCEDVLRMKEKYRLEVPMYTPEVLGYPFNIATTDEKERAETIAYLKKAIDVSRAI
                     GAPRVQLATGHAGHGTSRKKNRQNVVYVLKTLAEHAEKAGIDLVLEAVTLMESNVVIF
                     LDDLVELIDEVGSPRLKGMLDTVTPTVHWETFADYFEKLGENMEYIHFVDSDGRDQHH
                     FPIGEGKIPMEGLMAILHKYGYDGWLSSELVSPYGRDPELFAAQEIRNIRRVLAGR"
     gene            complement(1879..>2484)
                     /locus_tag="ACZPFK_10410"
     CDS             complement(1879..>2484)
                     /locus_tag="ACZPFK_10410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1406767.1"
                     /translation="NCLYDFCESRALPVKREDLLPYLGDGDRQAVSYICQMGGMDEEE
                     VLSAVRPQFWEGVKSLETRPGIRRYVTYAKKNGIKLAVVSNAGRDYLRSWLKRLLLDK
                     YFDCVVSRESALLPKPAPDLYLKAAELLHLEPARVIAVEDSVRGLRAASAAGMRAVAF
                     PHPASGPAVRALFPLCIDLAKVPPEKLIMQACTTPRQKSDL"
BASE COUNT          515 a          778 c          619 g          572 t
ORIGIN      
        1 cctgtttccc ggctgataac ccggatcccc gggcagttct ctgagatgaa ggagggcgat
       61 gatcggtttc gcggtcccaa aaagttcctt gatcgtctgc atcgtgatat tcctttcttc
      121 cttatcggat agcggccttt acggtctcga cccctctgcc aacagagggg ttggggtcga
      181 cataatgctt tctggaacag acctccagcg tgatggtccc atcaaaatcc tgctcttcca
      241 tctgtgagat gtactctttc aggggtaagt tcccatatcc ccagggcacc tgcccggatt
      301 cattgaggtg gatgtggacg atatccttgc caaatgtgtg aaaatagtcc tccagccgtt
      361 cgccctttac cgccatgtgg caggtatcca ccaacagctt cacattgggc aatcccaact
      421 cgtccagcat gcgctttgcg ctggggatat cccccaccag gtttgactcc acgatctgaa
      481 gcggctccag cgccaacgtg acgccctgct ccgcggcgaa cgccgcgatc tcggctatgg
      541 cgctgcgggc cagctcccag gcgaccttga ggccgtccgg ctgatcataa aacccccagc
      601 ccgggcagac gtgcagcaga ggcgcctgca ggatccggca cagttcagcc gcacgtttca
      661 tggatgagat ggatctgtcg cggatcaggt cgtcctctgc ggcgatgttg aacggataga
      721 cgacctgttc cgggcacacg cagaaacagt gcagcccccg tgaagcgatt ttttctccga
      781 tctttttgaa atcctcgtcg gtggcatcct ccagatggac atgggggttt gcagtccaca
      841 ggttgatgtt tttcaccccc atgccccggg ctgcgtccag aaagtagtcc agcgtgtaaa
      901 agaggtagtt catattcata atgccgaact gttcccattt taattttctc ataatctttc
      961 caccctcctc tgcagcgccc tcagcggcct gccagcaccc ggcgaatatt gcggatctcc
     1021 tgcgccgcaa acagctccgg gtcccggcca tagggggaga ccagctctga gctgagccaa
     1081 ccgtcatagc cgtatttgtg caggatcgcc atcagcccct ccatggggat cttcccctcc
     1141 ccgatcggga aatggtgctg atcgcgcccg tcgctgtcta cgaaatggat atactccata
     1201 ttttcaccca gcttttcgaa atagtcggca aaggtctccc aatgtaccgt cggcgtcaca
     1261 gtatccagca tccccttgag ccggggactg cccacctcgt cgatgagctc gacgagatca
     1321 tcgagaaaga taaccacatt gctctccatc agggtgaccg cctccaggac caagtcgatc
     1381 cccgcttttt ccgcgtgctc cgcaagcgtt ttcaacacgt agacgacatt ttgccggttc
     1441 tttttacggc tggtaccgtg gcccgcatgg cctgtggcca actgcacccg cggggctccg
     1501 atcgcccggg aaacgtcgat ggcctttttg agataggcta tggtttccgc ccgctccttt
     1561 tcatccgtcg ttgcgatatt aaacggatat cccagtactt ccggcgtata catggggacc
     1621 tcaaggcgat acttctcttt catacgcagc acatcctcac agcgcgccgc gtccatatca
     1681 tagggatacg cctgcggtcg ccctccccaa atctccacgc cgtcatagcc ctgctcggaa
     1741 gcgacctcga aaactttctc cagatgatac ctgcggaaca gtagggacag taacgcaatt
     1801 ttcactttca atcttccttt cttgtgctct tatacgcccg ctaaaattga gggccgtgaa
     1861 aaaatttgtc tccagccgtc atagatcgct cttttgtctg ggcgtcgtgc aggcctgcat
     1921 gataagcttc tcaggaggga ctttcgccag gtcgatacag agagggaaaa gcgctctcac
     1981 cgccgggccg gacgccggat ggggaaacgc caccgcgcgc atccccgcag cagacgccgc
     2041 acgcaggccg cgcacggaat cctccactgc gattacccgt gccggctcca agtgaagcag
     2101 ctctgccgct tttaaataga gatccggcgc cggctttggc aataacgcgc tctcgcggga
     2161 cacgacgcag tcaaaatact tgtccagtaa aagccttttc agccagctgc gcagataatc
     2221 ccggccggcg tttgacacga ccgccagttt tatcccattc ttttttgcat aagtgacata
     2281 tcggcgaatg ccgggccgcg tctccaggct tttcacaccc tcccaaaatt gcggccgcac
     2341 tgccgacagg acctcttcct catccatacc ccccatttgg caaatatagg agacggcctg
     2401 cctatcacca tcaccgagat aaggcaaaag atcctctctt tttaccggca gggcccggct
     2461 ttcgcaaaaa tcatacagac agtt
//
LOCUS       DBMDYA010000448         2471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9115_length_2471_cov_1.505485, whole genome shotgun sequence.
ACCESSION   DBMDYA010000448 DBMDYA010000000
VERSION     DBMDYA010000448.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9115_length_2471_cov_1.505485"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="ACZPFK_10415"
     CDS             <1..2134
                     /locus_tag="ACZPFK_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406768.1"
                     /translation="RFLKLTACAVSLALAAACVSPALAASQEVRKDENVFVILNADGS
                     VKEQIVSDWLHSDAGLSGAEDKSSLENIQVLKGDAAPTREGEALTWRTQENDVYYQGR
                     SGQTPPVSAAITYTLNGEEKTVEELLGQSGHLKITIALQNNEKTQKEIGGKLREVYTP
                     FATVVAADLPSGVFANVTAQHGTVQTDSSNQLACFVALPGLRQSFDGLLTGDLAGLED
                     LLLDEVSIEADVERFEMPTILLACATSMEELQQTADLSGLSGQLDELTEGTARLAEAA
                     LTLDGKMAELVSSYGTFDAGVDSALAGSQQVKTGADGLLAGAGDLLDGAAALQAGSGA
                     LAASLNDRLVPQLKNAAAQQSGLQTDMKALNTKLEELKKGGVTLPDEAVQQVAAAVTP
                     AVEEAFTTVAATAGGAASAETAAQIVARLAAQSQQYKDAAAAATANGDAAAAAQATAT
                     AAVLDNVIGSIQAAAADPSVQQAVAGSTAAAVQNSGEVAAAGQRIGAAVTGAVKAAAP
                     GVVGGVIGGLQGDMDKVNSEAEALLGGMEQLTGALYNSAEPEDPETVVGAANAIAGGA
                     DAVNGGAAQLRDGAAQLSGGADSLAQGLTKLSSSSKTVKAALGQFKDGTVQLAEGAGA
                     LDGGVQGMAGAEELANLPLIAEIADEMQAQADAYTSYTGAAEGTRASVKFVMKVEGPK
                     APQADAGEQAQAEQPTTFWERVKNLFQF"
     gene            2151..>2471
                     /locus_tag="ACZPFK_10420"
     CDS             2151..>2471
                     /locus_tag="ACZPFK_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406769.1"
                     /translation="MQFLIALFSGGAKMLFSPLCPHVLRLNAGKTKHPALKGRVFARE
                     HLPVRGSCALRIVDFTPQPRILWFLPCELPARAGNLREVSQRRSAAFALRDGGGFERG
                     AVQKK"
BASE COUNT          468 a          762 c          889 g          352 t
ORIGIN      
        1 acgctttttg aaattgacag cctgcgccgt cagcctggcg ctggccgcag cctgtgtcag
       61 cccggctctg gccgcctcgc aggaggtccg caaggacgag aacgtctttg tcatcctgaa
      121 cgccgacggc tcggtaaaag aacagatcgt cagcgactgg ctgcacagcg acgcggggct
      181 ttccggcgct gaggacaaga gcagcctgga gaatatccag gtgctgaaag gggacgctgc
      241 gcccacccgg gagggggagg ccctcacctg gcggacgcag gaaaacgacg tctactacca
      301 ggggcgctcc ggccagaccc cgccggtgag cgcagccatc acctacactc tgaacggcga
      361 agaaaagacc gtcgaggagc tgctggggca aagcggccat ctgaagatca ccatcgcgct
      421 gcaaaacaac gaaaaaacgc aaaaggagat cggcggcaaa ctgcgggagg tttatacccc
      481 gtttgcgacc gtcgtcgcgg cggacctgcc cagcggggtc tttgcaaacg tcacagccca
      541 gcacggcacc gtgcagaccg attcgagcaa tcagcttgct tgctttgtgg ccctgccagg
      601 cctgcggcag agctttgacg gcctgctcac cggcgatctg gcggggctag aagacctgct
      661 gctggacgag gtgtccattg aggcggatgt ggagcgcttt gagatgccca ccatcctgct
      721 cgcctgcgcc accagcatgg aagagcttca gcagaccgca gacctctcgg gcctgtccgg
      781 ccagctggac gagttgaccg agggcacggc ccggctggcg gaggccgccc ttaccctgga
      841 cggcaaaatg gctgagctgg tgagcagcta cggcacgttt gacgccggtg tcgacagcgc
      901 cctcgccggc tcgcagcagg tcaaaacggg ggcggacggc ctgctggcgg gcgcgggcga
      961 cctgctggac ggcgccgcgg cgctgcaggc gggcagcggg gcgctggccg caagcctgaa
     1021 cgaccggttg gtgccgcagc tgaaaaacgc ggcggcccag cagagcgggc tgcagacgga
     1081 tatgaaagcc ctgaatacca agctggaaga gctgaaaaag ggcggcgtca ccctgccgga
     1141 cgaagcggtg cagcaggtgg ctgccgcggt gacgcctgcg gtggaggagg ccttcaccac
     1201 cgtggcggcc acggcggggg gcgcggcgag cgccgagacg gcggcgcaga tcgtggcccg
     1261 gcttgcggcc cagtcgcagc agtacaagga cgccgcggct gcggccactg caaacggcga
     1321 cgctgcggct gcggcacagg ccacggcgac ggccgcggtg ttggacaatg tgatcggctc
     1381 gatccaggcc gcggcagcgg acccgtccgt gcagcaggcg gtcgcgggca gcaccgctgc
     1441 ggccgtgcag aacagcggtg aggtcgccgc cgcggggcag aggatcggcg ccgccgtgac
     1501 cggtgcggtc aaggcggcgg ccccaggtgt ggtcggcggt gtgatcggcg gtctgcaggg
     1561 ggatatggac aaggtcaaca gcgaggccga ggccctgctg ggcggcatgg aacagctcac
     1621 cggggccctg tacaacagcg ccgagccgga ggaccctgag accgtggtcg gggccgccaa
     1681 cgccatcgcc ggcggcgcag acgcggtaaa cggcggcgcg gcccagctca gggacggcgc
     1741 ggcccagctg agcggcgggg cggacagcct tgcccagggc ctgaccaagc tgtccagctc
     1801 cagcaaaacg gtcaaggccg ccctggggca atttaaggac ggcacggtgc agctggccga
     1861 gggtgcgggc gccctggacg gcggcgtgca ggggatggcc ggggccgaag agctggcgaa
     1921 cctgccgctg atcgccgaga tcgcagacga gatgcaggcc caggccgacg cctacaccag
     1981 ctacaccggc gcggccgagg ggacccgtgc cagcgtcaag ttcgtcatga aagtcgaggg
     2041 gcccaaggcg ccgcaggcgg atgccgggga gcaggcacag gcggaacagc ccaccacgtt
     2101 ctgggagcgg gtcaaaaacc tgtttcagtt ttaaagcggt cttttgattt ttgcagttcc
     2161 tgatagcgct gttttccggc ggggcgaaaa tgcttttttc accgctctgt ccgcatgttt
     2221 tgaggttgaa cgccggcaag acaaaacatc ctgctttaaa agggcgcgtt ttcgcaagag
     2281 agcatctccc tgtcagagga agctgcgcgc tgcgcatcgt cgacttcacc ccgcagccac
     2341 ggattttgtg gtttctcccc tgcgagctgc ccgcgcgggc aggcaatctg cgtgaagttt
     2401 cacagcgccg cagcgcggct ttcgcgctca gggatggcgg cggttttgaa agaggcgcag
     2461 tacaaaaaaa g
//
LOCUS       DBMDYA010000449         2460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9152_length_2460_cov_1.167020, whole genome shotgun sequence.
ACCESSION   DBMDYA010000449 DBMDYA010000000
VERSION     DBMDYA010000449.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9152_length_2460_cov_1.167020"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(299..1756)
                     /locus_tag="ACZPFK_10425"
     CDS             complement(299..1756)
                     /locus_tag="ACZPFK_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015563.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR1406770.1"
                     /translation="MGEKKQEKKGLQISAKTFIITLAVLLFIVVLAGVLTLVLPQGSF
                     EYALVDGRETVVPGTYHLVEAARLPVWHWLTAPVEVLFSDEGVTAAMIIVFLLLIGGT
                     FQVLDESRVLNYIITAVIHKFGHKKYRLMLLMVFLCMLVGSTIGMFEETVTLVPITVA
                     LALSLGWDSLVGLGMSILSVGFGFASATFNPFTVGITQKLAGLPAFSGLALRAAVFAL
                     VYAMLSAFLLRYAKRVEKDPRSSLVWEHDLSIRDKYLSSVDEGILAEREIRRAALVFG
                     CGFGVVLAYILAGFFVEGLSDYSLPVMAVCFTVGGLAAGRIAGLKKGLAKNFFSGMGS
                     IAPSVLLILLALSAKFILTEGGIMDTILNAAYGMISGTSPYLAVLLLLAFVLLLQFFI
                     GSATAKAFLIMPLVLPLVDLVGLTRQTAVQAFLFGDGFTNMIFPTNAVLLISIGLVGV
                     SYGKWFKWTWKLQLGTLLLSVAVLMGCVALGYGPF"
     gene            2063..>2460
                     /locus_tag="ACZPFK_10430"
     CDS             2063..>2460
                     /locus_tag="ACZPFK_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406771.1"
                     /translation="MAEKRAVGKRGAFPRGLLVLLLGALAVRVLLVATTDGYLYDTNT
                     FSAWAGLLNERGLSGFYTGGFFADYPPGYMFVLWAAGRVTEALGLAALSGPALLVLCL
                     APVLCDLGLAALLYRVALPRGGRRGALLAAA"
BASE COUNT          458 a          830 c          757 g          415 t
ORIGIN      
        1 gatgctcttt ttcctgtgca ccgcgggcac ggcgctggtc ttttcgctgc tctatttctt
       61 cacctattcc ctcaccgccc gcacctatta caagctggtg ggccacacca tctgacccgc
      121 cggctcaacg ccaaggagga acgatatgaa gctgaggcat tttttgatcc tgggcgccct
      181 ggccgcagcc atcctgctgc tctttcgcag ggcgcaccgc ttttgcaacc cggagctggg
      241 ataacaacaa aacagggccg ctgctttgca gcggccctgt tttgccgaga acagcttttt
      301 aaaacggccc gtagcccagc gccacgcagc ccatcagcac cgccacgctg agcagcaggg
      361 tgccgagctg cagcttccag gtccacttga accacttgcc gtacgacacc cccaccaggc
      421 cgatggagat cagcagcacc gcgttggtgg ggaagatcat gttggtgaag ccgtccccga
      481 acaaaaacgc ctgcaccgcc gtctgccggg tgaggccgac cagatccacc aggggcagca
      541 ccagcggcat gatgagaaaa gcctttgccg tggccgagcc gatgaagaac tgcagtagca
      601 gcacaaaggc cagcagcagc aacacggcca gatacggcga ggtgccggag atcataccgt
      661 aggcggcgtt gaggatggtg tccatgatgc cgccctcggt gaggatgaac ttggcgctga
      721 gggccagcag gatcagcagc acgctgggcg cgatggagcc catgccggag aaaaagtttt
      781 tcgccagccc ctttttcagc cccgcgatgc gcccggcggc caggcccccc accgtgaagc
      841 agactgccat caccggcagc gaatagtcgg acaggccctc cacaaaaaag cccgccagga
      901 tataggccag caccacgccg aagccgcagc cgaacaccag cgccgcccgg cggatctccc
      961 gctcggccag gatgccctcg tccacgctgg acaggtattt atcccggatg gagaggtcgt
     1021 gctcccacac cagcgaggaa cgggggtcct tttccacccg ctttgcatac cgcagcaaaa
     1081 aggcggacag catggcgtag accagcgcaa agaccgccgc ccgcagcgcc agcccgctga
     1141 aagcgggcag cccggccagc ttttgggtga tgcccaccgt aaaggggttg aacgtggcgg
     1201 acgcaaagcc gaagcccacc gagaggatgc tcatgcccag ccccaccagc gaatcccacc
     1261 ccagcgagag ggccagggcc accgtgatgg gcaccagggt gaccgtctcc tcaaacatgc
     1321 cgatggtgga gcccaccagc atgcacaaaa agaccatcag cagcatcagc ctgtattttt
     1381 tgtgcccgaa tttgtggatc accgccgtga tgatatagtt gagcacccgg ctctcgtcca
     1441 gtacctgaaa ggtgccgccg atgagcaaaa gaaagacgat gatcatggct gcggtgacgc
     1501 cctcgtcgct gaacagcacc tccaccggcg cggtcagcca gtgccagacc ggcagccgcg
     1561 cggcctccac caggtgatag gtgcccggca ccacggtctc gcgcccatcc accagcgcgt
     1621 actcaaagct gccctggggc agtaccagcg tcagcacccc ggccagcacc acgatgaaca
     1681 gcagcacagc cagcgtgatg ataaaggtct tggcgctgat ctgcagcccc tttttttcct
     1741 gctttttctc ccccataacc ggctcccctc ggcggcccga cgggccgccc gttgcttttg
     1801 ggcgccgccg cgccccctgt agccggcgcg cgggccctgt tttcctccat actacccgac
     1861 cggggcggcc ctgtcaaatg ccgggcggcg tttccctttg cctgccgtct caaaaatgct
     1921 ataatggaaa aaaccggcgg ggcggcgtct gcccccggcg gagacgcgcc cgcgccgggg
     1981 ccgcggcttt ggcctgcggc cccggaggac gtatcctaat ttaaaattaa cccgcgcacg
     2041 gcgcggaaaa tgaggtggga ctatggcgga aaagcgggct gtgggaaaga gaggcgcttt
     2101 cccccgcggg cttttggtgc tgctgctggg cgccctggcc gtgcgggtgc tgctggtggc
     2161 gacgaccgac ggctatctct acgacaccaa cacgttttcc gcctgggcgg gcctgctgaa
     2221 cgagcggggc ctgtccgggt tttacaccgg cggctttttt gccgactatc cccccggcta
     2281 tatgttcgtg ctctgggccg cgggccgggt gaccgaagcg ctgggtctgg cggcgctctc
     2341 cgggccggcg ctgctggtcc tctgcctggc gccggtgctc tgcgacctgg gcctggcggc
     2401 tctgctctac cgcgttgctc tgccccgggg tgggcggcgc ggggcgctgc tggcggcggc
//
LOCUS       DBMDYA010000450         2459 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9156_length_2459_cov_1.491094, whole genome shotgun sequence.
ACCESSION   DBMDYA010000450 DBMDYA010000000
VERSION     DBMDYA010000450.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9156_length_2459_cov_1.491094"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="ACZPFK_10435"
     CDS             complement(<1..530)
                     /locus_tag="ACZPFK_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HflX-like GTP-binding protein"
                     /protein_id="HIR1406772.1"
                     /translation="MEELKQGPRTRAALFAADCGDRDIDRSLAELRALAESAGMEVLL
                     EIVQRRDAPDTGCYLGEGRLAEAKRLCADNEVAVCVFDDELTGTQIKNLEDVLELPVV
                     DRTLLILDIFAARAVTNEGKLQTELAALQYRLPRLSGLRDGLSRQGGGGAGGGGARRG
                     AGESKLEYDRRHLRRR"
     gene            complement(637..1212)
                     /locus_tag="ACZPFK_10440"
     CDS             complement(637..1212)
                     /locus_tag="ACZPFK_10440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1406773.1"
                     /translation="MKRHPGTKTLQTPRLLLRRFAEADAQAMFEGWAGRPEVVRFMRY
                     NAHESVAETRAILRGWAFAYEEEEDFYTWAVTLRETGALAGSIGLTQHEGVPEPGYTL
                     APAFWGQGIVREALAAVLAYLADEGGAERFAACHAVANPASGAVLRSAGFQPEGEGSY
                     ESFDHTRVWPCIYYRLEAKDLVRGAPYRIMG"
     gene            1455..2321
                     /locus_tag="ACZPFK_10445"
     CDS             1455..2321
                     /locus_tag="ACZPFK_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1406774.1"
                     /translation="MKKIISLGIALSLMAALLAGCGGTDSSSGGGASASPTQAPGAAL
                     SGSVGTNGSTSMEKVIGSLSEAFMGQNGEVKVTYDPTGSGTGIQAVVDGTTDIGLSSR
                     ALKEEEKAKGLQETTVALDGIAIIVNKDNPVEDLTLEQIAGLATGEIANWSEVGGSDA
                     AVVMEGREAGSGTRDGFESIVGVSDACSYANESTSTGAVIANVAANPNAIGYASLSAV
                     DDTVKAITVEGVAASEETVLDGSYKIQRPFIFVTKQGAALSAAAQAFFDFATSADANE
                     IIAGAGAVPVAE"
BASE COUNT          438 a          851 c          777 g          393 t
ORIGIN      
        1 atgcgccgcc gcagatgacg gcggtcatat tccagcttgc tctcgcccgc gccgcgccgg
       61 gccccgccgc cgcccgcgcc gccgccgccc tggcgggaca ggccgtcccg caggccggaa
      121 aggcgcggca gccggtactg cagggcggcg agctcggtct gcagcttgcc ctcattggtc
      181 accgcgcggg ccgcaaagat gtccaggatc agcagcgtgc ggtccaccac cggcaattcc
      241 agcacgtcct ccaggttttt gatctgggtg ccggtgagct cgtcgtcaaa gacgcagacg
      301 gccacctcgt tgtccgcgca aaggcgcttt gcctccgcca gccgcccctc gcccagatag
      361 cagccggtgt ccggcgcgtc gcgccgctgc acgatctcca gcagcacctc catcccggcg
      421 ctctcggcca gggcgcgcag ctcggccagg ctgcggtcga tatcccggtc cccgcaatcg
      481 gccgcgaaca gggcggcgcg ggtgcggggg ccttgcttca attcttccat cctcatcctc
      541 cattttgccg ctttgcgcgg atgcggcccg ccgccggggc aaaaacgcgc ccgattgccc
      601 cggcccgtcc gcctgcggct ttgccgcgcg ccgccctcag cccataatcc gatagggcgc
      661 gccgcgcacc agatcttttg cctctaagcg atagtaaata cagggccaca cgcgggtgtg
      721 gtcaaagctc tcatagctgc cctcgccctc gggctgaaag cccgccgagc gcagcaccgc
      781 gccgctggcc gggtttgcca cggcgtgaca ggcggcaaag cgctcggcgc cgccctcatc
      841 ggccagatag gccagcaccg ccgccagcgc ctctctgacg atcccctgtc cccaaaaggc
      901 gggggccaga gtgtagcccg gctcaggcac cccctcgtgc tgggtgaggc cgatggaacc
      961 ggccagggcc ccggtctccc gcagggtgac ggcccaggtg taaaagtcct cctcttcctc
     1021 ataggcaaag gcccagccgc gcaggatggc ccgggtctcc gccacgctct catgtgcgtt
     1081 gtagcgcatg aagcgcacca cctccggccg cccggcccag ccctcaaaca tggcctgggc
     1141 gtccgcctcg gcaaagcggc gcagcagcag ccggggcgtc tgtaaagttt tggtgccggg
     1201 atggcgtttc atacgccgcc cccctcctct tgtgatataa gtagtattta ttgtagccgc
     1261 aaatgtgcaa aaaagcaatg aaaagccgcg ccgcaggggc agggggcctt taccctctct
     1321 ttacaaaatg catacacaaa gctggtgttc cgcacaaagc ctgggctggt aaaatggcat
     1381 cgtagcgaag agctgcggaa agctcgcgtc agaccttaca gagctttgag tgatgaaagg
     1441 aaaaggggat cgttatgaaa aagatcattt ctctcggcat tgcattatct ctcatggccg
     1501 cgctgctcgc cggctgcggc ggcacggatt ccagctcggg cggcggcgcc tccgcctccc
     1561 ccacccaggc gcccggcgcg gcgctgtccg gctcggtggg caccaacggc tccacctcga
     1621 tggaaaaggt catcggcagc ctctccgagg cctttatggg ccagaacggg gaggtgaaag
     1681 tcacctatga ccccaccggc tccggcaccg gcatccaggc ggtcgtcgac ggcaccaccg
     1741 acatcggcct gtcctcccgc gcgctgaaag aggaggaaaa ggcaaagggc ctgcaggaga
     1801 ccaccgtcgc gctggacggc atcgccatca tcgtcaacaa ggacaacccg gtggaagacc
     1861 tgaccctgga gcagatcgcc gggcttgcca ccggcgagat cgccaactgg agcgaggtcg
     1921 gcggcagcga cgccgcggtg gtcatggagg gccgcgaggc gggcagcggc acccgcgacg
     1981 gctttgagag catcgtgggc gtctcggacg cctgcagcta cgccaacgag tccacctcca
     2041 ccggcgcggt catcgccaac gtggccgcca accccaacgc catcggttat gccagcctgt
     2101 ccgcggtgga cgacaccgtc aaggccatca ccgtcgaggg ggtcgccgcc agcgaggaga
     2161 ccgttttgga cggcagctac aagatccagc gcccgtttat ctttgtgacc aaacagggcg
     2221 cggccctgag cgcggcggcc caggcgttct tcgacttcgc caccagcgcc gacgcgaacg
     2281 agatcatcgc gggcgcgggc gccgtgccgg tcgcggaata agcgcggttt ttagaaagag
     2341 atcacggaaa ctccccgggg acgcctgccg tccccggggc agttgccggg ttcggaggtt
     2401 tttgtgaaaa cgaaaaaatt tgtggaaaaa ctgatgaacg ccgtcttttt cctctgcgg
//
LOCUS       DBMDYA010000451         2450 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9194_length_2450_cov_1.455939, whole genome shotgun sequence.
ACCESSION   DBMDYA010000451 DBMDYA010000000
VERSION     DBMDYA010000451.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9194_length_2450_cov_1.455939"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            67..366
                     /locus_tag="ACZPFK_10450"
     CDS             67..366
                     /locus_tag="ACZPFK_10450"
                     /inference="COORDINATES: protein motif:HMM:NF027405.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1406775.1"
                     /translation="MDLDFCKEFIQPELLALVPALNFLGTVLKRSASVPDKNIPLLLG
                     GAGVLLSAVWVVGQNTPRDLSQVLAAVFTALVQGLLCAAGSVYLHQLMKQRKKPS"
     gene            complement(543..1862)
                     /locus_tag="ACZPFK_10455"
     CDS             complement(543..1862)
                     /locus_tag="ACZPFK_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1406776.1"
                     /translation="MRQKPNILVFFTDQQRADSCGCYGSPNGLTPFLDSLGEDGVVFD
                     NAVSCQPVCGPARAVIQTGQFATRNGCYRNAIDLPVDGQTLARRMKAAGYKTGYVGKW
                     HLAGTGVEPVPQELRAGYEDYWITADLLEFSSGPGSGFVFDKQNRKREFEKYRVDAIT
                     DYAVETLRAHDPADPLFLFVSYLEPHHQNNQNRYVAPDGYAERYRDAWVPDDLVALDK
                     PDADWRANLPDYYGCIRRLDENLERLVGVLKERGMYEDTVIVFTCDHGSHFRTRNGEY
                     KRSCHEASVHIPLVFRGGAFRGGRHAPQAVSLVDLAPTLLDLAGAAPAEGMDGRSFAA
                     MARGDIPGWDEAVLIQISESEVARALRTPRYKYCVTAPHKNPWLDASSDLYVEECLYD
                     LQQDPWELKNLVADPASRQIKEGLRALLLEKIAASGEKTPVILPLER"
     gene            complement(1902..>2450)
                     /locus_tag="ACZPFK_10460"
     CDS             complement(1902..>2450)
                     /locus_tag="ACZPFK_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1406777.1"
                     /translation="GEIEIRLGLEVEYYPRYFEQLLRAVEGYPIEYFLLGQHYLQDEM
                     GAPYSGALTRDPRILEGYCSQVIGGMETGCYSCLAHPDLINFHGDGALYEQQMRRLCR
                     EALRHGLPLEINFLGISESRSYPDERFWKIAGEQGCPVVFGCDAHTPAAVLNPEAERR
                     AQELVRRYGLQRVEKLTLRAPV"
BASE COUNT          376 a          848 c          783 g          443 t
ORIGIN      
        1 aactaaaaca aagggccgcg gacggcgccg ccgggcgtca gggctcggcc ggggggagtg
       61 aatgccatgg atctggattt ctgcaaggag tttatccaac ccgagctgct ggcgctggtg
      121 ccggcgctca actttctcgg caccgtgctc aagcgctcgg cgtcggtgcc ggacaagaac
      181 atcccgctgc tgctgggcgg cgccggtgtg ctgctctcgg ccgtctgggt ggtggggcag
      241 aacacgcccc gggacctctc ccaggtgctg gccgccgttt ttaccgctct ggtccagggg
      301 ctgctctgcg cggcgggcag cgtctatctg caccagctga tgaaacagcg caaaaagccg
      361 tcctgagggg ggcgcttttc ccgggcggcg gcgcggcaaa gaggccgggc ggggtttgct
      421 cctttcaaga gcaagccccg cccggccttt tacttcgctg cctgttcccg cccggccctc
      481 ggcctccccc aaagcctctt tgaggagggg tgcatcttcc ccctagccag cggcgggccc
      541 gctcagcgct ccagcggcag gatcaccggc gtcttttcgc ccgaggccgc gatcttttcc
      601 agcaacaggg cgcgcagccc ctccttgatc tgccggctgg cggggtccgc caccaggttc
      661 ttcagctccc acggatcctg ctgcagatcg tagaggcact cctccacgta caggtcgctg
      721 ctcgcgtcca gccaggggtt tttgtgcggc gcggtgacgc agtacttgta gcgcggcgtg
      781 cgcagcgcgc gggcgacctc gctctcgctg atctggatga gcaccgcctc gtcccagccc
      841 gggatgtccc cccgcgccat ggcggcgaaa gagcggccgt ccatcccctc ggccggcgcg
      901 gcccccgcca gatccagcag cgtgggggcc aggtccacca gcgacaccgc ctgcggggcg
      961 tgtctgccgc cccggaacgc cccgccgcgg aacaccagcg ggatgtgcac gctggcctca
     1021 tggcaggagc gcttgtactc gccgttgcgg gtgcggaagt gggagccgtg gtcgcaggtg
     1081 aagacgatga cggtgtcctc gtacatcccc cgctctttca gcacccccac cagccgctcc
     1141 aggttttcgt ccaggcggcg gatacagcca taatagtcgg gcaggttggc cctccagtcg
     1201 gcgtccggct tatccagggc caccaggtcg tcgggcaccc aggcgtcccg gtaccgctcg
     1261 gcgtagccgt cgggggccac atagcggttc tggttgttct ggtggtgcgg ctcgagatag
     1321 gacacgaaca ggaacagcgg gtcggcgggg tcgtgggccc gcaaagtctc gacggcatag
     1381 tcggtgatgg cgtccacccg gtatttctca aactcacgct tgcggttctg cttgtcaaag
     1441 acaaagccgc tgcccggccc ggaggaaaac tccagcaggt cggccgtgat ccagtagtcc
     1501 tcatatccgg cgcgcagctc ctgcggcacc ggctccaccc ccgtgccggc cagatgccat
     1561 ttgcccacgt agccggtctt gtagccggcg gctttcatgc ggcgcgccag cgtctgccca
     1621 tccaccggca ggtcgatggc gttgcgataa cagccgttgc gggtggcgaa ctggccggtc
     1681 tggatcaccg cccgcgccgg gccgcacacc ggctggcagc tgacggcatt gtcaaagacc
     1741 acgccgtcct cccccagtga atccaaaaac ggggtcagcc cgttggggct gccgtagcag
     1801 ccgcagctgt cggctcgctg ctggtccgtg aaaaagacga gaatattcgg tttctgtctc
     1861 atatcctgct cctttttgca tttccgtccc gcgccgtgcg ctcaaaccgg ggcgcgcagc
     1921 gtcagcttct ctaccctttg caggccgtat ctgcgcacca attcctgcgc ccgccgctcc
     1981 gcttcggggt ttaagaccgc ggccggcgtg tgggcgtcgc agccgaagac caccgggcag
     2041 ccctgctccc cggcgatctt ccaaaaccgc tcgtccgggt agcttctgct ctcgctgata
     2101 ccgagaaagt tgatctccag cggcaggccg tgccgcagcg cctcgcggca caagcggcgc
     2161 atctgctgct cgtacagggc gccgtcgccg tgaaaattga tcaggtccgg gtgggcgagg
     2221 cagctgtagc agcccgtctc catacccccg atcacctggc tgcaatagcc ctctaaaatg
     2281 cgcgggtccc gggtgagcgc gccgctgtag ggcgcgccca tttcatcctg cagatagtgc
     2341 tgccctagca aaaaatattc gatgggatag ccctccaccg cccgcagcag ctgctcgaaa
     2401 tagcgggggt agtactccac ctccagcccc aggcggatct ctatctcgcc
//
LOCUS       DBMDYA010000452         2443 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9223_length_2443_cov_1.241674, whole genome shotgun sequence.
ACCESSION   DBMDYA010000452 DBMDYA010000000
VERSION     DBMDYA010000452.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9223_length_2443_cov_1.241674"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="ACZPFK_10465"
     CDS             complement(<1..379)
                     /locus_tag="ACZPFK_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1406778.1"
                     /translation="MRAVVHPGPACGSVQAPASKSVAHRAMLAAALAEGETVLEGVSP
                     SADLSATLAAARAFGAAVRPLAPDAWQMRGVPLPQAPSGPVDCGESGSTLRFLIPLLA
                     LTGAEVTLTGRGRLPQRPQEVYQQ"
     gene            complement(379..1395)
                     /gene="aroF"
                     /locus_tag="ACZPFK_10470"
     CDS             complement(379..1395)
                     /gene="aroF"
                     /locus_tag="ACZPFK_10470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1406779.1"
                     /translation="MIITMKKLAPQEEIDRMVQSIELKGLHVTMISGTDYNVFGVVGD
                     TTILDDRKLSASPYVDNVQRVSAPYKLANRVFHPADTVVDVSGVKIGSSEPIVVMGGP
                     CSIESEAHTDEMARLVSEAGGRIFRGGAYKPRTSPYAFQGLGTEGIRYMAAAGRKYGL
                     PIVSELMSIDKLDEFVENVDLIQIGARNMQNFDLLKEIGKTKKPVLLKRGLANTIEEW
                     LMSAEYIMAGGNENVVLCERGIRTFEKYTRNTLDLSVIPIIKQRSHLPIVIDPSHATG
                     DRKLVESMSLAAIAAGADGLIIETHCDPPHALSDGAQCLYPSQFAFLIEKGREIARVV
                     GRDL"
     gene            complement(1436..1879)
                     /gene="aroQ"
                     /locus_tag="ACZPFK_10475"
     CDS             complement(1436..1879)
                     /gene="aroQ"
                     /locus_tag="ACZPFK_10475"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR1406780.1"
                     /translation="MRILVLNGPNINLLGLREPGVYGSDSYADLLELIRRTGEEEEVE
                     TEVLQSNHEGDLVDGIQQALGRVDGLVLNPGAYTHTSIALLDALKAVRLPAVEVHISA
                     VERREAFRQVSYIRPACFLTITGEGIAGYARAIRELNAHILAAQA"
     gene            complement(1860..>2443)
                     /locus_tag="ACZPFK_10480"
     CDS             complement(1860..>2443)
                     /locus_tag="ACZPFK_10480"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1406781.1"
                     /translation="AQAKTAAELFLGRPLPDEEIDRVHRGLQAERSNCVLIAMPGAGK
                     TTVGSRAARMLGKTFVDTDDEITAHTGRTPAQIIEQEGEAAFREIESRVIAEIAKKGG
                     QLIATGGGAVTRAENMQRLRQNGVILFIDRELSLLATSGRPLSNNFEALRQRYIERYP
                     LYNRYCDLKLDNDETIETAARRVEEAFYAYFGA"
BASE COUNT          367 a          801 c          829 g          446 t
ORIGIN      
        1 gctgctgata gacctcctgc gggcgctggg gcagcctgcc gcggccggtg agggtgacct
       61 cggcgccggt gagcgccagc agggggatca aaaagcgcag ggtggagccg gattccccgc
      121 aatccaccgg ccccgacggg gcctggggca gaggcacgcc gcgcatctgc caggcgtccg
      181 gggccagcgg gcgcaccgcc gcaccaaagg cccgcgccgc cgccagggtg gcgctcaggt
      241 ccgccgaggg cgagacgccc tccagcaccg tttccccctc ggcgagcgcc gccgccagca
      301 tggcccggtg ggcgacgctc ttggaggccg gggcctgcac gctgccgcag gcgggcccgg
      361 ggtggacgac ggcccgcatc agagatcgcg ccccaccacc cgggcgatct cacggccttt
      421 ttcgatcaaa aaggcaaact ggctggggta gaggcactgc gcgccgtccg ataaggcgtg
      481 gggcgggtcg cagtgggtct cgatgatgag cccatccgcc ccggcggcga tggcggccag
      541 ggacatcgac tccaccagct tgcggtcgcc ggtggcgtgg ctggggtcga tgacgatggg
      601 cagatggctg cgctgcttga tgatggggat gaccgacagg tccaacgtgt tgcgggtgta
      661 tttttcaaag gtgcggatgc cccgctcgca gaggacgacg ttctcattgc cgccggccat
      721 gatatactcg gcgctcatca gccattcctc gatggtattt gccaggccgc gcttgagcag
      781 caccggcttt ttggtcttgc ctatctcttt gagcaggtcg aagttctgca tgttgcgcgc
      841 cccgatctgg atcagatcca cgttctcgac gaattcatcc agcttgtcga tgctcatcag
      901 ctcgctgaca atgggcaggc cgtattttct gcccgctgcc gccatgtagc ggatgccctc
      961 ggtgcccagg ccctgaaagg cgtaggggct ggtgcgcggc ttgtaggcac cgccgcgaaa
     1021 aatgcgtccg cccgcctcgc tcaccaagcg cgccatctcg tcggtatggg cctcgctctc
     1081 gatggagcag gggccgccca tcaccacgat gggctcgctg ctgccgatct tgacgccgga
     1141 gacgtccacc accgtgtcgg ccgggtggaa cacgcggttg gccagcttat agggggcgga
     1201 cacccgctgt acattgtcca catagggcga ggcggacagc ttgcggtcgt ccaggatcgt
     1261 ggtgtcgccc accacgccga agacgttgta atcggtgccg ctgatcatgg tgacgtgcag
     1321 gcctttcagc tcgatgctct gtaccatgcg gtcgatttct tcctgcgggg cgagcttttt
     1381 catggtgatg atcataatca ggtatctccg tttctgtgtt ggggttgcgg ggcggtcagg
     1441 cctgcgccgc caggatgtgg gcattcagct cccggatggc ccgggcgtag cccgcgatgc
     1501 cctcgccggt gatggtcaaa aagcaggcgg gccggatgta ggacacctgc cggaacgcct
     1561 cccgccgctc gacggcggag atgtgcacct ccaccgccgg cagacgcacg gccttgagcg
     1621 cgtccagcag ggcgatgctg gtgtgggtgt aggccccggg gttgagcacc aggccgtcca
     1681 cccggcccag ggcctgctgg attccgtcca ccaggtcgcc ctcgtggttg gactgcagaa
     1741 cctcggtctc aacctcctcc tcttcgccgg tgcgccgtat cagctccagc aggtcggcat
     1801 agctgtcgct gccgtaaacg ccgggctcgc gcaggcccag caggttgatg ttgggcccgt
     1861 taagcaccaa aatacgcata aaatgcctcc tccacccgcc gcgcggcggt ctcgatggtc
     1921 tcgtcgttgt ccagcttcag gtcgcagtag cggttgtaga gcggatagcg ctcgatatac
     1981 cgctggcgca gggcctcaaa gttgttggag agcggccgcc ccgacgtggc cagcagcgag
     2041 agctcccggt cgatgaagag gatgaccccg ttctgccgca gccgctgcat attttcggcc
     2101 cgggtcaccg caccgccgcc ggtggcgatg agctggccgc cctttttggc gatctcggcg
     2161 atgaccctgc tctcgatctc gcgaaaggcg gcttcgccct cctgctcgat gatctgcgca
     2221 ggggtgcgcc cggtgtgggc ggtgatctcg tcgtcggtgt ccacaaaggt cttgcccagc
     2281 atgcgggcgg cccggctgcc caccgtggtc ttgcccgcgc cgggcatggc gatgagcacg
     2341 cagttggagc gctcggcctg caggccgcga tgtacccggt cgatctcctc gtccggcagg
     2401 ggcctgccca aaaacagctc cgccgccgtc ttggcctggg cca
//
LOCUS       DBMDYA010000453         2439 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9238_length_2439_cov_1.315227, whole genome shotgun sequence.
ACCESSION   DBMDYA010000453 DBMDYA010000000
VERSION     DBMDYA010000453.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9238_length_2439_cov_1.315227"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /gene="ftsH"
                     /locus_tag="ACZPFK_10485"
     CDS             <1..1343
                     /gene="ftsH"
                     /locus_tag="ACZPFK_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1406782.1"
                     /translation="KYREIGAKLPKGALLVGPPGTGKTLLAQAVAGEAGVPFFSISGS
                     EFVEMFVGMGAARVRDLFKQAGEKAPCIVFIDEIDAIGKKRDNSGMGGNDEREQTLNQ
                     LLTEMDGFDAGKGVVILAATNRPETLDPALLRPGRFDRRIPVELPDLAGREAILKVHS
                     KDVRIAPNADFNAVARATSGASGADLANIINEAALGAVKAGRKAVVQADLDEAVETVI
                     AGYQRKNAVISTKDRLIVSYHECGHALVAALQKHSAPVHKITIVPRTSGALGYTMQVD
                     EEEQVLMSRETILDKIATFTAGRAAEELIFGSVTSGASNDIEQATKLARAMVTQLGMT
                     DEFDMTALANQQSAYLGGDASLVCSADTAARVDQKVVEIIREQHQKALGLLRENKAKL
                     HELARELYERETLTGDEFMEILARPVPLPAAVEEAAAAPTSHTTSPAGQEETDA"
     gene            1340..1735
                     /locus_tag="ACZPFK_10490"
     CDS             1340..1735
                     /locus_tag="ACZPFK_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406783.1"
                     /translation="MNGFRNFFGNRYGIDNLNITLMVVALALSIISRFFPRSVLLPAL
                     GMLLIVLVFYRALSTKLVVRRSENEKFLGLWYAVRDWFQHPHFGGGVRDGKRCFKCPN
                     CGQKCRVPKGKGMIAITCPRCGQDFIKKT"
     gene            1800..>2439
                     /locus_tag="ACZPFK_10495"
     CDS             1800..>2439
                     /locus_tag="ACZPFK_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1406784.1"
                     /translation="MGGWFTTEQIDGETFALSEYGHPEEAHCYLLCGGSRALLIDTGL
                     GVGDLKSEVERLTSLPILVATTHVHWDHIGGHGQFRQIAVFEKEAAWLSGGFPLPLAV
                     VRRNLAAGGCRFPEGFDPEQYRIFQGGAGRLLRDGEVIRLGGRSVQVLHTPGHSPGHC
                     CFYEPDRGFLYTGDLIYKGCLDAFYPTTDPKAFLRSAQKAAALPARRLLPGHH"
BASE COUNT          440 a          810 c          811 g          378 t
ORIGIN      
        1 gcaaataccg ggagatcggc gccaagctgc ccaagggcgc gctgctggtg ggccctccgg
       61 gcaccggcaa aacgctgctg gcgcaggcgg tcgccggcga ggcgggcgtg ccgttcttct
      121 ccatctcggg cagcgagttc gtggagatgt tcgtgggcat gggcgccgct cgggtgcgcg
      181 acctgttcaa gcaggccggt gaaaaggctc cctgcatcgt ctttatcgat gagatcgacg
      241 ccatcggcaa aaagcgcgac aacagcggca tgggcggcaa cgacgagcgc gagcagaccc
      301 tcaaccagct gctcaccgag atggacggct tcgacgcggg caagggcgtg gtgattttgg
      361 ccgccaccaa ccggcccgaa acgctggacc ccgccctgct gcggcccggc cgctttgacc
      421 ggcgcatccc ggtggagctg cccgacctgg cgggccgcga ggcgattttg aaagtgcact
      481 ccaaagacgt gcgcatcgcc ccgaacgcgg acttcaacgc cgtcgcgcgc gccaccagcg
      541 gcgcctcggg cgcggatctc gccaacatca tcaacgaggc cgccctgggc gcggtgaaag
      601 ccgggcgcaa ggcggtggta caggcggatc tggacgaggc ggtggagacc gtcatcgccg
      661 ggtaccagcg caaaaacgcg gtcatcagca ccaaggaccg gctgatcgtc agctaccacg
      721 agtgcggcca cgccctggtg gcggcgctgc aaaagcactc ggccccggtg cacaagatca
      781 ccattgtgcc gcgcaccagc ggcgcgctgg gttataccat gcaggtggat gaagaggagc
      841 aggtgctgat gagccgggag accatcctgg acaagatcgc cactttcacc gcgggccgcg
      901 ccgccgagga gctgatcttc ggctcggtca ccagcggcgc ctccaacgat atcgagcagg
      961 ccaccaagct ggcgcgggcc atggtcaccc agctgggcat gaccgacgag ttcgacatga
     1021 cggcgctggc aaaccagcag agcgcctacc tgggtgggga cgcctctctg gtctgtagcg
     1081 ccgacacggc cgcccgcgtc gaccaaaagg tggtggagat catccgcgag cagcaccaaa
     1141 aggcgctggg gctgctgcgg gagaacaagg ccaagctgca cgagctcgcc cgcgagctgt
     1201 atgagcgcga gaccctcacc ggcgacgagt tcatggagat cctcgcccgc ccggtgccgc
     1261 tgcccgccgc cgtggaagag gcggccgcgg cccccacatc ccacaccaca tcgcccgcag
     1321 ggcaggagga aactgacgca tgaacggctt tcgcaatttt ttcggcaacc gctacggcat
     1381 cgacaacctg aacatcaccc tgatggtggt ggcgctggcg ctctccatca tctcccgctt
     1441 tttcccccgc tcggtgctgc tgccggcgct gggcatgctg ttgatcgtgc tggtctttta
     1501 tcgcgcgctg tccaccaagc tggtggtgcg ccggtctgaa aacgaaaaat ttctcgggct
     1561 ctggtacgcg gtgcgggatt ggttccagca tccgcatttc ggcggcgggg tccgggacgg
     1621 taagcgctgc ttcaaatgcc ccaactgcgg ccaaaagtgc cgcgtgccca aaggcaaggg
     1681 catgatcgcc atcacctgcc cccgctgcgg gcaggatttc atcaaaaaaa cctgagctct
     1741 ccggcggacg cccaaaggcg cgccgcaaaa aacgggctca caaaaaggag cgtgcgcgcg
     1801 tgggaggctg gttcacgaca gagcagatcg acggcgagac ctttgccctc agcgaatatg
     1861 gccacccgga ggaggctcac tgctatctgc tgtgcggcgg cagccgggcc ctgctgatcg
     1921 acaccgggct gggggtgggc gacctcaaga gcgaggtgga gcgcctgacc tctttgccga
     1981 ttttggtggc gacgacccac gtgcactggg accacatcgg cgggcacggg cagttccggc
     2041 agatcgccgt ctttgagaaa gaggcggcct ggctttccgg cgggttcccg ctgccgctcg
     2101 ccgtcgtgag gcggaatctg gccgcggggg gctgccgctt ccccgagggg ttcgacccgg
     2161 agcaataccg catcttccag ggcggcgccg gacgcctcct gcgcgacggc gaggtcattc
     2221 gcctcggggg ccgcagcgtc caggtgctgc acacccccgg tcactccccc ggccactgct
     2281 gcttttatga gccggacagg ggctttctct acacggggga cctgatttac aagggctgtc
     2341 tcgacgcctt ttatcccaca accgacccaa aggccttttt gcgcagcgcc caaaaagcgg
     2401 cggcgctgcc ggcgcgccgc ctgctgcccg gccaccaca
//
LOCUS       DBMDYA010000454         2434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9255_length_2434_cov_1.771967, whole genome shotgun sequence.
ACCESSION   DBMDYA010000454 DBMDYA010000000
VERSION     DBMDYA010000454.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_2434_cov_1.771967"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPFK_10500"
     CDS             complement(<1..572)
                     /locus_tag="ACZPFK_10500"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014759.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR1406785.1"
                     /translation="MDKLLIIDGSNLLFQMFYGMPARIVNRDGKAIQGTLGFVGAAIK
                     MIKMTAPTHLVALFDGEQQNSRTELLAEYKANRPDFSQVEAKDNPFSQLGDVYRALTF
                     MGVRHAEVQGVETDDVVASYVKAYGAQMSVVIASFDSDFFQLIGDNVAVLRYRGKKSV
                     LCDAAYLREKLDVAPCRYADFKSLTGDSAD"
     gene            complement(825..2186)
                     /locus_tag="ACZPFK_10505"
     CDS             complement(825..2186)
                     /locus_tag="ACZPFK_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406786.1"
                     /translation="MRISDLLSLSFENLRRRKGRTTLTIIGVIVGTCSIVVMVSLGIA
                     MNVGFDEMISQWGDLTQIQVYNWSGGNSDVPKLDDKMVAELDALEHVKVATPLYNPRY
                     LNAQVFAGKGDRYFMDAYNIVGIYPEAIKELEYELISGKYLDEATTSSKKLPVLVGQY
                     AGYGFTDSKKRGEKAYRWQGQTDAQGNPLDPFVDVNKDKLTLKTVKQEDNSKQLTYEL
                     QVTGVMLEDYKKGYVTSQGIVMDLNTLKRLEKEYMKANGIKDSNYDKQGYDNIYVKVD
                     DVDNVEAVEKAIQDYGYDTYSMSSERDRMREQSQMIQMILGGLGAVSLFVAALSIANT
                     MTMAIYERTREIGVMKVLGCKLGKIRQMFLLEAAMIGFVGGVLGVGISYALSFLMNHF
                     GPMLQNVLGSILPMYGSKMSVIPPWLALVGILFSTAIGILSGIMPANRAVKISALEAI
                     RHE"
     gene            complement(2173..>2434)
                     /locus_tag="ACZPFK_10510"
     CDS             complement(2173..>2434)
                     /locus_tag="ACZPFK_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406787.1"
                     /translation="DEKGQEKTLEKEFSVEVQQNDMGGMDPGIMDPGMMDPTMGQPTG
                     GMPWWGWVLIAAGAVVVVVVIVVIVKKQKAKKQRLEDEDEDI"
BASE COUNT          461 a          759 c          657 g          557 t
ORIGIN      
        1 ttgtcggcgc tgtcgccggt gagggacttg aaatccgcgt accggcaggg ggcgacgtcc
       61 agcttttctc tcagataagc ggcgtcgcag aggacggatt ttttgccccg gtagcgcagc
      121 accgccacgt tgtcgccgat cagctgaaaa aagtcgctgt caaaggacgc aatcaccacg
      181 gacatctgcg ccccataggc ctttacatag cttgcaacca cgtcgtcggt ctcaactccc
      241 tggacctcgg catggcggac gcccataaac gtcagggcgc gatacacgtc tcccagctgg
      301 gaaaatgggt tgtctttggc ctccacctgc gagaagtcgg gccggttcgc cttatactcc
      361 gccagcaatt ccgtgcggct gttttgctgt tcgccgtcaa agagcgccac caaatgggtg
      421 ggggccgtca tcttgatcat tttgatcgcc gcgcccacaa agcccaaggt accctgtatc
      481 gcctttccat cccggttcac gatgcgcgcg ggcatcccgt aaaacatttg gaacaacagg
      541 tttgagccgt cgatgatcaa cagcttgtcc atataacccc gcctttcaag cttaaaatcg
      601 cccgctgaat cgggcttgcc ccgcgttttt tctaacgtgt tacacaagct ggggaaaaac
      661 agctttatct tgacagcttg tttctcgttg ctgggcggca accaaaacga atgaaagctc
      721 ccgcaacaca gaacgccttt ccctgccgca agcagaggga aaggcgttct gtgtgttttt
      781 tgtaccgctt cttccggcgg cgcgcccttt gggcgtgcaa ggcctcactc gtgccggatt
      841 gcctccagcg cggagatttt caccgcccgg ttggccggca tgatgccgga caggatgccg
      901 atggcggtgg agaacaggat gcccaccagc gccagccagg gcgggataac cgacatcttg
      961 gagccgtaca tgggcaggat gctgcccaat acattttgca gcatggggcc gaaatggttc
     1021 atcagaaaag agagggcata gctgatgccc acgcccagca ccccgcccac aaagccgatc
     1081 atcgcggcct ccagcaggaa catctggcga atcttgccca gcttgcagcc cagcaccttc
     1141 atgacgccga tctcgcgggt gcgctcgtag atcgccatgg tcatggtgtt ggcgatcgag
     1201 agcgccgcca caaacaggga cactgcgccg agaccgccca aaatcatctg gatcatctgc
     1261 gactgctcgc gcatccggtc gcgctcgctg ctcatggaat aggtgtcgta gccgtagtcc
     1321 tgaatggcct tttccaccgc ctccacgttg tccacgtcgt ccactttcac atagatgtta
     1381 tcgtagccct gtttgtcgta attggagtcc ttgatgccgt ttgctttcat gtactctttt
     1441 tccagccgct tcagggtgtt caagtccatg acgatgccct gcgaggtgac gtagcccttt
     1501 ttgtagtcct ccagcatcac gccggtgacc tgcagctcat aggtgagctg cttcgagtta
     1561 tcctcctgct tcaccgtttt gagggtcagc ttatccttgt tgacgtccac aaacgggtcc
     1621 agcgggttgc cctgggcgtc ggtctgcccc tgccagcgat aggctttctc gccccgcttt
     1681 ttgctgtcgg taaagccgta gcccgcgtac tgccccacca gcaccggcag ctttttgctt
     1741 gaggtcgtgg cttcatccag gtatttgccg ctgatgagct catattccag ctctttgatg
     1801 gcctcgggat agatacccac gatgttgtag gcgtccatga aataccggtc gcccttgccg
     1861 gcaaaaacct gcgcattgag ataacgggga ttgtaaaggg gcgtggccac cttgacgtgc
     1921 tccagcgcgt cgagctccgc caccattttg tcgtccagct ttgggacgtc gctgttgccg
     1981 ccgctccagt tatagacctg tatctgggtc aggtcgcccc actgggagat catctcgtca
     2041 aagcccacgt tcatggcgat acccagcgag accatgacca cgatggaaca ggtgcccacg
     2101 atgacgccga tgatggtgag cgtcgtgcgg cccttgcggc ggcgcaggtt ctcaaagctg
     2161 agcgagagaa gatcagatat cctcatcttc gtcctccaga cgctgctttt tcgctttttg
     2221 ctttttcacg atcacgacga tcacgaccac gaccaccacc gcgccggccg cgatcagcac
     2281 ccagccccac cagggcatgc cgccggtggg ctggcccatg gtcgggtcca tcatgccggg
     2341 gtccatgatc cccgggtcca tgccgcccat atcgttctgc tgtacctcga ccgagaactc
     2401 cttttccagc gtcttctcct gacccttttc atcc
//
LOCUS       DBMDYA010000455         2430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9266_length_2430_cov_1.566337, whole genome shotgun sequence.
ACCESSION   DBMDYA010000455 DBMDYA010000000
VERSION     DBMDYA010000455.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_2430_cov_1.566337"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(177..1694)
                     /locus_tag="ACZPFK_10515"
     CDS             complement(177..1694)
                     /locus_tag="ACZPFK_10515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1406788.1"
                     /translation="MAKFFLGIDIGTYESKGVLMDGECRIKAVHTERHGLENPRENYF
                     EHDAEKVWWGDFCRITHALMQETGLKSSDIAAVGASTLGADLVAVDEECRPLRKAILY
                     GIDARAGKEIEGLNKKYGLEKVLEFNGRPLCSNDVPPKILWLKNNEPLIHEKAYKFLT
                     GSSFITAKLTGRYVIDRFLAYGAFTPLYDTRDGSVNMEYCEEFCRPDQLARVCDTTDI
                     AGVVTGRAAQETGLMEGTPVIAGTDDSAAEAISTGVLEKGDMMVQIGSSLYLIGLCGK
                     LIRDTRVWSGGFLIPGTFSVQGGTNNAGTLTRWYRDNIFFDKAGEEDEGGPNAYDAML
                     RGIEEIPPGSGGLITLPYFAGERTPINDPQARGMLFGLTLRHTRQHMYRSALEAVGYS
                     LAQHFDIFRKNGVDIRTIYAVGGGTKAPVWMQILSDILGVEIVTADVTVGASFGDAVM
                     AAIGVGHFAGFTEVRNYIRPGRRYYPNERAHCLYEPYVKMFGELYQRNRDLMHLL"
     gene            complement(1712..>2430)
                     /locus_tag="ACZPFK_10520"
     CDS             complement(1712..>2430)
                     /locus_tag="ACZPFK_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIR1406789.1"
                     /translation="NRLEDLVACARRELRALQDGGVDGILFSNEFSFPYLDHLEHVTT
                     AAMAYIIGRLKEEIRVPFGVHAISDPMATIELAAAVEAGFVRGVFTGGYVGEAGIRSK
                     NAGEYARKKREFGMDDLLMFYMINAESDGDLSGRSLEVIARATVFKCQPAGLCMSGIH
                     AGEDADTSLLQRIRDAVPGTPVFSNTGTRKGNVAEKLKYCDGAFVGTSFKKDGKFENF
                     VDYARVKEFMDTVHANRGDL"
BASE COUNT          500 a          781 c          581 g          568 t
ORIGIN      
        1 gttcacagac agcaagccct tttggctgct tgatcatatc tttgtctaac tttttgggtg
       61 cagttcattt cgcgcatcgg cccgttgtct tttttacttt tatcgctttc catctttcag
      121 aatatcgctg tgcattccgg caaaagcacg gtacttccat cgctcgtctc tcgccctcac
      181 aaaagatgca tcagatcccg gttgcgctgg taaagctccc cgaacatttt cacatacggc
      241 tcgtagaggc aatgagccct ctcattggga taatagcgcc tgccgggacg gatataattt
      301 cggacctcag taaaccccgc aaaatgtccc accccgatcg cagccattac ggcatcgcca
      361 aaggaggcgc ccaccgtgac atccgcggtc acgatttcaa cccccaagat atcggacagg
      421 atctgcatcc acaccggggc tttggtcccg ccgccgaccg cataaatcgt gcggatatcc
      481 actccgtttt tgcgaaaaat gtcgaagtgc tgcgccagag aataccccac cgcctccagc
      541 gcggaccggt acatatgttg ccgggtgtgc cgcagcgtca ggccaaacag catccctctc
      601 gcctgagggt cattgattgg cgtgcgctct ccggcaaaat agggcagggt gataagccct
      661 cccgaccccg gggggatctc ctcgatcccc ctcagcatcg cgtcataggc attgggtccc
      721 ccctcgtcct cctctcccgc cttgtcaaaa aagatattgt cccgatacca gcgggtcaag
      781 gtgcccgcgt tattggtccc cccctggaca ctgaaagtgc ccggaatgag aaatccaccg
      841 ctccagacac gcgtatcgcg gatcaatttg ccgcacagcc cgatcagata gagagaagaa
      901 ccgatctgga ccatcatatc ccctttttcc agcacgcctg tgctgatcgc ctcagccgcg
      961 gaatcatctg tgccggcgat taccggggtt ccttccataa ggccggtctc ctgcgccgcc
     1021 cggccggtga cgacgcctgc gatatcggtc gtatcacata cgcgcgccag ctggtccggc
     1081 cggcagaact cttcgcagta ttccatattg accgagccgt cgcgggtatc atagagcggg
     1141 gtaaaggccc catatgcaag aaagcggtcg attacatagc gtcccgtaag ttttgccgtg
     1201 ataaaagaag agccggtcaa aaatttatac gccttttcgt ggatcagcgg ctcgttgttt
     1261 ttcagccaga gaattttggg gggcacgtcg ttggaacaga gcgggcgacc gttaaattcc
     1321 agtacctttt ccagcccata tttcttattc agcccctcaa tctcctttcc ggcccgcgcg
     1381 tcaatcccgt acaggatcgc tttgcgcagc ggccggcatt cttcatccac tgcaaccagg
     1441 tccgccccca gcgtgctggc tcctaccgcc gcaatatcag agcttttcag gcctgtttcc
     1501 tgcataaggg cgtgggtaat cctgcaaaaa tctccccacc acaccttttc cgcatcgtgc
     1561 tcaaaataat tttcccgggg attttccagc ccatggcgct ctgtgtggac cgctttgatc
     1621 cggcattctc cgtccatcaa tacccctttg ctctcgtagg taccgatatc aatgcccaga
     1681 aaaaactttg ccatcccttg cccctttccg ctcacaggtc gccgcggttg gcgtggaccg
     1741 tgtccataaa ctctttgact cttgcatagt cgacaaaatt ttcaaacttg ccgtcttttt
     1801 taaaagaggt ccccacaaaa gccccgtcgc agtacttcag cttttccgct acgtttccct
     1861 ttctcgtgcc cgtgttgctg aagaccggcg ttcccggcac tgcgtcccgt atcctctgca
     1921 gcagactggt gtccgcatct tcaccggcgt ggatgccgga catgcaaagc ccggcaggct
     1981 ggcatttaaa gaccgtagcc cgcgcaatca cctccaggct gcgtccgctc agatcgccat
     2041 ccgactctgc attgatcata taaaacatca gcaggtcatc catgccaaac tccctttttt
     2101 tgcgggcata ttcacctgca ttttttgagc ggatcccagc ctcgcccaca tagccgcctg
     2161 tgaacacccc gcgcacaaac cccgcctcca ccgcagcggc cagttcgatc gtcgccatag
     2221 ggtcgctgat ggcatgtact ccaaagggca cacggatctc ctctttcagc cgcccgatga
     2281 tatatgccat cgcggcggtg gtgacgtgtt ccagatggtc cagataggga aagctgaatt
     2341 cgttggaaaa caggatgccg tccacaccgc cgtcctgcag ggcgcgaagc tcccgccggg
     2401 cacaggctac aaggtcttcc agcctgtttc
//
LOCUS       DBMDYA010000456         2429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9277_length_2429_cov_1.120704, whole genome shotgun sequence.
ACCESSION   DBMDYA010000456 DBMDYA010000000
VERSION     DBMDYA010000456.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9277_length_2429_cov_1.120704"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="ACZPFK_10525"
     CDS             <1..325
                     /locus_tag="ACZPFK_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1406790.1"
                     /translation="SAYSFMIFNLLCAPCFAAMGAIKREMNNGKWTAAAIGYMCGFAY
                     AAALIVYQLGGLITGQVGFGVGTVAALAVLAALLFLLFRPNRYNSDYKRGSAPSLKLD
                     GKVKV"
     gene            444..599
                     /locus_tag="ACZPFK_10530"
     CDS             444..599
                     /locus_tag="ACZPFK_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1406791.1"
                     /translation="MNLPTLVIGLVVLAAVAAAVASMVRKKKKGGGCGCGCSGCASAG
                     ICHPQKR"
     gene            1399..2085
                     /gene="sfsA"
                     /locus_tag="ACZPFK_10535"
     CDS             1399..2085
                     /gene="sfsA"
                     /locus_tag="ACZPFK_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR1406792.1"
                     /translation="MYYLDVRPARFVSRPNRFVARVEQEGRIFAAHVKNTGRCAELLL
                     PGARVYLEYSRNPARKTPCDLIAVEKERPGLPPLLINMDSAAPNAVAAEWLAAGGLGP
                     LEGLRPEYPLGDSRFDFYAEERGRPVLIEVKGCTLERDGVALFPDAPTLRGLKHVREL
                     TALARRGWRCCVLFVIQMKGPRRFAPNRATQPAFAQALQDAAAAGVEVLARDCLVAPD
                     ALTIDGEVEV"
BASE COUNT          464 a          745 c          693 g          527 t
ORIGIN      
        1 gtccgcctac agctttatga tcttcaacct gctctgcgcc ccctgcttcg ccgccatggg
       61 cgccatcaag cgcgagatga acaacggcaa gtggacggcg gccgccatcg gctatatgtg
      121 cggcttcgcc tacgccgccg ccctgatcgt ctatcagctg ggcggcctca tcaccggcca
      181 ggtcggcttc ggcgtcggca ccgtggcggc gctggcggtg ctggcggcgc tgctcttcct
      241 gctcttccgc cccaacagat acaactcgga ttacaagcgc ggctcggccc cctcgctcaa
      301 gctggacggc aaggtcaagg tatgaggccg caaaaggccc tgccttccct gtcccgcccg
      361 caccggggct ggaacgcccg tgagagcgcc cgggttcgct ccgcatacca aggcattttt
      421 cgcaaagaaa ggactgagcg tttatgaatc tccccaccct cgtgatcggc cttgtcgtgc
      481 tggccgcggt ggccgccgcg gtggcgagca tggtgcgcaa aaaaaagaaa ggcggcggct
      541 gcggatgcgg ctgctccggc tgtgccagcg ccggtatctg ccacccgcaa aagcgctgaa
      601 ccacgcaaat atcctccctg aaagagacgg ctgcttgcag ccgtctcctc ctttttgcac
      661 tcgcttcccg ccatgccgga acgaccgcct gccgcaaagc gcgctggcga tccgctttaa
      721 aatgtgatag catgacaaga gaaaaaagaa aaagagatgc ctttttggcc gtgcccagac
      781 aaggaaccga cgctgcgaaa aagacagttg gggtacatag ccgagttggc tgaaagcgca
      841 attgcgcact ggaggtactg tgtccaactc tatagggctc atgtcggtgt gagagaaacc
      901 aacttgtccg tcatacgggg gggggtgcgc cgatggccgt ttttcttgtg ccgcgtgctg
      961 cggcgggcaa gcgcccgccc tgtccgcccg ccttgcctgc ccagcctgct ccgccgccct
     1021 ttttcccgca ccgcggacgg tctctggttt tgctccgtaa aaaatgccct ttcggcgttg
     1081 atccgcaaag cattgctgcg caaaatattg ttctgtaaag ctctgggacg ccttacactg
     1141 catcccaaat tgccacttca ctacctaaaa agagaatggc aaaatcattc tctcacggtc
     1201 cctcgctgcg cttttgtata gagaatattt tgcggtgacc cgtacacccc cacgatttgg
     1261 gatacggagt ggacaaaagc caagggcgca gtcctgcatg aattgcgccg cagatttgaa
     1321 tctgctctgc gccaggctcg tacggctaaa ctactatttt cttgtccggg tccggccttt
     1381 tccggggagg tgtttgtttt gtattatctc gacgtgcggc ccgcgcgctt tgtcagccgc
     1441 cccaaccgtt ttgtcgcgcg ggtggaacag gaggggcgga tttttgccgc ccatgtgaag
     1501 aacaccggcc gctgcgccga gctgctgctg cccggcgcca gggtctatct ggagtacagc
     1561 cgcaacccgg cccgcaagac cccctgcgac ctgatcgcag tggaaaagga acgccccggc
     1621 ctgccgcccc tgctcatcaa tatggactcg gccgccccca acgccgtggc ggccgagtgg
     1681 ctggccgccg gcggcctggg gccgctggag gggctgcgcc ccgaatatcc cctgggagac
     1741 tcccgctttg acttttacgc cgaagagcgc ggccgcccgg tgctgatcga ggtgaaaggc
     1801 tgtacgctgg agcgggacgg agtggccctc ttccccgacg cgcctaccct gcgggggctg
     1861 aagcacgtgc gcgagctgac cgccctcgcc cgccggggct ggcgctgctg cgtgctcttt
     1921 gtcatccaga tgaaagggcc ccgccggttc gccccgaacc gcgccaccca gcccgccttt
     1981 gcccaggccc tgcaggacgc cgcggcggca ggggtggagg tgctggcccg cgactgcctg
     2041 gtcgccccgg acgccctgac catcgacggg gaggtggagg tatgagcgga agttatcttg
     2101 gatgtttcgg taatattgaa gatgcgtttt ttatcgttga taatcttgaa taacttgcat
     2161 aaattgttaa taagattgcg cctaacaaag cccttagaat cggtgaccgg gatattcttt
     2221 ttataccttc cctaaattta tctcaggttg cactctgttc ttttatgcaa tctatgatac
     2281 tcccataacg tgtgcaagca gcttactgta ttgccatgct ctacaactac atgaatatta
     2341 aacatcatgc tgcgcagctt tgacatgcac aaaatgtcta atggtttaag atatcagaaa
     2401 tgtttaatct ttttcaatat ggttttgca
//
LOCUS       DBMDYA010000457         2424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9302_length_2424_cov_1.205768, whole genome shotgun sequence.
ACCESSION   DBMDYA010000457 DBMDYA010000000
VERSION     DBMDYA010000457.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9302_length_2424_cov_1.205768"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="ACZPFK_10540"
     CDS             <1..1744
                     /locus_tag="ACZPFK_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1406793.1"
                     /translation="DMSAPFDPGPAAAGAGAGQDGARAPFDLDLGPAAPKAEKKQDGL
                     FEHPAPDVEQLIKKAVAPRAEAAKPAIDPSGQVLLSPGAPGQNAYRYPPLDLFERGSA
                     ARGGADAAELKRNADLLVNTLASFGVQTKLMDISRGPSVTRYELQPAAGVKISRITGL
                     ADDIALNLAAAGVRIEAPIPGKAAVGIEVPNKHASAVDMHSILSSNEFKNSKSPIVIP
                     LGKDIAGSVQVADLTKMPHLLIAGSTGSGKSVCINAIIISIVYRCSPEDVKMILIDPK
                     VVELSDYNGIPHLLMPVVTEPRKASGALGSAVAEMERRYQLFAQNNVRDIKSYNKLAS
                     GSAELEHLPYIAIVIDELADLMMVAGKEVEDYICRIAQKARAAGMHLIVATQRPSVDV
                     ITGLIKANIPSRIAFAVSSQIDSRTILDGAGAEKLLGMGDMLFMPVGANKPVRIQGTY
                     VKDGEITAVIDYVKQDNAAQYDENMILDMEKYASAPSGKGTAAGDGEDSDAHDPMLQA
                     AIETVIEANMASTSLLQRKLKLGYARAARIMDTMEELHVIGPYEGSKPRAVLISRQQF
                     IEMFTNGSVEETGG"
     gene            2041..>2424
                     /locus_tag="ACZPFK_10545"
     CDS             2041..>2424
                     /locus_tag="ACZPFK_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR1406794.1"
                     /translation="MEKFLPITGDEMRARGWQYCDFVVVSGDAYVDHPSFGPAIISRL
                     LESQGYRVGFLSQPDFTTPESFKEFGLPRYAFLIGAGNVDSMVAHYSVAKVRRTEDEY
                     TPGGRAGKRPDRAATVYTRLAKQAYP"
BASE COUNT          463 a          785 c          829 g          347 t
ORIGIN      
        1 tgatatgagc gcgccctttg accccgggcc cgcggcggcg ggtgcgggcg ccggacagga
       61 cggcgcccgc gcgccgtttg atctggacct ggggcccgcc gcccccaaag cggagaaaaa
      121 gcaggacggc ctgttcgagc acccggcgcc ggatgtggag cagctcatca aaaaagcggt
      181 ggcccccagg gcggaggcgg caaagcccgc catcgacccc agcgggcagg tgctgctgtc
      241 gcccggcgcc ccggggcaga acgcctaccg ctatccgccg ctggacctct ttgagcgcgg
      301 cagcgcggcg cgcggcggcg ccgacgcggc ggagctcaag cgcaatgcgg acctgctggt
      361 gaacacgctg gccagctttg gggtgcagac caagctgatg gatatcagcc gcggcccctc
      421 ggtcacccgc tatgagctgc agcccgcggc gggggtcaag atcagccgca tcaccggttt
      481 ggccgacgat atcgcgctga acctggccgc ggcgggggtg cgcatcgagg cgccgatccc
      541 cggcaaggcg gcggtgggca tcgaggtgcc caacaagcac gccagtgcgg tggacatgca
      601 cagcatcctc agctcgaatg agttcaaaaa cagcaagagc cccatcgtca tcccgctggg
      661 caaggatatc gccggcagcg tgcaggtggc ggatctcacc aaaatgcccc atctcttgat
      721 cgccggctcc accggcagcg gcaaatcggt ctgcatcaac gccattatca tcagcatcgt
      781 ctaccgctgc agccccgagg acgtcaagat gatcctcatc gaccccaagg tggtggagct
      841 gtccgactac aacggcatcc cacatttgct gatgccggtg gtcaccgagc cgcgcaaggc
      901 cagcggcgcg ctgggcagcg cggtggcgga gatggagcgc cgctatcagc tgtttgcgca
      961 gaacaacgtg cgcgacatca aaagctacaa caagctggcc tcgggcagcg ccgagctgga
     1021 gcacctgccc tacatcgcca tcgtcatcga cgagctggcc gacctgatga tggtcgcggg
     1081 caaggaggtg gaggactata tctgccgcat cgcccaaaag gcgcgcgccg caggtatgca
     1141 tctgatcgtc gccacccagc gccccagtgt ggacgtcatc accggcctca tcaaggccaa
     1201 catccccagc cgcatcgcct ttgcggtgtc cagccagatc gacagccgca ccatcctgga
     1261 cggcgcgggc gccgaaaagc tgctgggcat gggggacatg ctgttcatgc ccgtgggcgc
     1321 gaacaagccg gtgcgcatcc agggcaccta tgtcaaggac ggcgagatca cggcggtgat
     1381 cgattacgtc aagcaggaca acgccgccca gtacgacgag aacatgattt tggatatgga
     1441 aaaatatgcc tccgcaccct ccggcaaggg cacggcggcg ggggacgggg aggacagcga
     1501 cgcccacgac cccatgctgc aggccgccat cgagacggtc atcgaggcca atatggcctc
     1561 cacctcgctg ctgcagcgca agctgaagct gggctacgcc cgcgcggcgc gcatcatgga
     1621 caccatggag gagctgcatg tcatcggccc ctatgagggg tccaagccgc gggcggtgct
     1681 catcagccgc cagcagttca tcgagatgtt caccaacggc tcggtggagg agaccggcgg
     1741 gtaaaaaggg cagagacagg ggcttccggc cggcagggac ggaagccctg cgccgttatg
     1801 accgccctac gccgggtcgg cgggctcggt gcgcccgacg ccgggcccgc agcccacggg
     1861 ttcggggcgg gagtgggcgg gatatgcccc tgtgggtgcc gggcctgcac cccagacggg
     1921 gcgcggcggg atacggcgca ggagatcccg ctgctcgccg gggagctcat agcctgcaac
     1981 ccgccgggga tgcgggggga tacgcagaag cgtgaaagag agaaacaaca cggagggact
     2041 atggaaaaat ttttgcccat cacgggcgac gagatgcggg cgcggggctg gcagtactgc
     2101 gattttgtgg tggtctcggg cgacgcctat gtggaccatc ccagcttcgg cccggccatc
     2161 atctcccggc tgctggagag ccagggctat cgggtgggct ttttgtccca gccggatttc
     2221 accacccccg agagcttcaa ggagttcggc ctgccgcgct acgcctttct catcggggcg
     2281 ggtaatgtgg acagtatggt ggcccactac tcggtagcaa aggtgcggcg cacagaggat
     2341 gagtacaccc ccggcggcag ggcgggcaag cggccggacc gggccgccac cgtctacacg
     2401 cggctggcaa agcaggccta tccc
//
LOCUS       DBMDYA010000458         2423 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9307_length_2423_cov_1.630491, whole genome shotgun sequence.
ACCESSION   DBMDYA010000458 DBMDYA010000000
VERSION     DBMDYA010000458.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9307_length_2423_cov_1.630491"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(580..1647)
                     /locus_tag="ACZPFK_10550"
     CDS             complement(580..1647)
                     /locus_tag="ACZPFK_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1406795.1"
                     /translation="MSTYREFRAEAKKKKRRRNFQRCCLILLIAVLLGGLTWFVVQVA
                     GRIGTAASSSAAPASSQAAQSLATDAVGDDGNAGAASSSSPVWQGIGPIQQTINNFQI
                     ISPDRRMISLPENGRVDLSYFDDATFVGDSITTGWAVYRAADGLLPNAHVVAEISTSP
                     PVGGVQWVHDRNKPGQYVYDPMQAIADTVPKKVYVMFGTNMLVNQSEATEDKLISDYS
                     AFIDDLGVRVPGCEVYIQSILIPTAAGTASRPGLNPERINRVNDRLAALAFEKGCHYL
                     NVQEYLCRDGVLNWDIAAKDGIHMNQDGYRAWLDYLATHTAYSAAASYVGGTPYPAAP
                     AQQAPAEPAAETPAPTDAPAA"
     gene            complement(1815..>2423)
                     /locus_tag="ACZPFK_10555"
     CDS             complement(1815..>2423)
                     /locus_tag="ACZPFK_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1406796.1"
                     /translation="VALNTDSPELQALVAQRYPETVLVERPQELCGDTVPKMAVFQQT
                     LAEMERRGGGRYDHLIDLDITSPIRRESDVAGAFAAAEARADLDLVFSVAQARRNPYF
                     NMVKVVGDHVEKVIESPYTARQQAPAIYDLNASIYVFRRDFLAENETGILWDGKCGIY
                     EMFDSGILDIDSEEDYLLMEVIARHLYATAPEFAAVRDNIRA"
BASE COUNT          412 a          802 c          764 g          445 t
ORIGIN      
        1 aggggactgg ccggatggcc gccaaaaata gcctgcggct attttttata ttgtaccacg
       61 cccaagacgc ggtcacccgg caaaaagttt gaaagttttg tggaaacttt tcttgtcccc
      121 tgcttttccc actccccgca cttcccgcat tctccgcact cccctttctc ctccccggct
      181 gcacacccgc ggcgcgcgcg gcaaaacatc tttcgccccg ttgggcgcac cggggcaaaa
      241 aaacattgat ttcgaaccga cggctttgac gcgccgcaga cgcagaaaag gttggcaatc
      301 cctgtcgatt gccaaccttt tctgcactgt gcttttttaa gaccaccgtc ccaaagccgc
      361 cccacacccc ggcaggaaaa gctgcggcgc cgcgctggta cgcttcaccc gggggttgcc
      421 tgcccccgcg ggcagcccgc agggcaaaac cgcaaaagcg ctcttgcggt gactgcgggg
      481 tgaatgtgac ggctctctgc gctctcccct ggggacaagg tttccctgct gcaacgcgcc
      541 ccccaccgga cgggcggtcc gaactcgttc attcagaggt caggcggccg gcgcatccgt
      601 cggggcgggc gtttccgccg cgggctcggc cggcgcctgc tgcgcgggcg cggcgggata
      661 gggcgtgccg cccacataac tggctgcggc gctgtaggcc gtgtgggtgg ccagatagtc
      721 cagccaggcg cggtagccgt cctggttcat gtggatgccg tccttggcgg cgatgtccca
      781 gttgagcacg ccgtcgcggc agagatactc ctgcacgttc aggtaatgac agcccttttc
      841 aaaggccagc gccgccagac ggtcgttcac ccggttgatg cgctccgggt tcaggcccgg
      901 ccttgaggcc gtgcccgcgg cggttgggat gaggatggac tggatataca cctcgcaccc
      961 cggcacgcgg acgcccaggt cgtcgataaa ggcgctgtaa tcgctgatga gcttatcctc
     1021 ggtggcctcg ctctggttga ccagcatgtt ggtgccgaac atcacataga cctttttggg
     1081 caccgtgtcg gcaatggcct gcatggggtc gtagacatac tgacccggct tattgcggtc
     1141 gtgcacccac tgcacgccgc ccacgggggg cgaggtgctg atctcggcca ccacatgggc
     1201 gttgggcagc agcccgtcgg cagcgcggta aacggcccaa ccggtggtga tcgagtcgcc
     1261 cacaaaggtg gcgtcatcaa aatacgacag gtccacccgc ccgttttcgg gcaaagagat
     1321 catgcgccgg tccggcgaga tgatctgaaa attgttgatg gtctgctgga tcgggccgat
     1381 gccctgccac accggcgagg aggagctcgc cgccccggca ttcccgtcgt ccccgacggc
     1441 gtccgtcgcc agggactggg ccgcctgcga ggaggcgggc gcggcggagg agctcgccgc
     1501 cgtgccgatg cggcctgcga cctggacgac gaaccaggtc aggccgccca gcaggacggc
     1561 gatgagcagg atgaggcagc agcgctgaaa attgcggcgg cgctttttct ttttggcctc
     1621 ggcccgaaat tcgcgatacg tggacaatct atcatctcca tgggcacaaa gggccccgtt
     1681 tttttgatgc gcgcggcaga gccgcccccc gggccgcccg ccgtctgttc ttatcatacc
     1741 ccgcagcgtg caaaacttca acgcacaggc cgtggaaaaa tctgttgttt gccgtcgaaa
     1801 ccgccccggc gcgctcaggc gcggatattg tcgcgcacgg ccgcaaactc cggcgccgtg
     1861 gcgtatagat ggcgggcgat gacctccatc agcaggtagt cctcctccga gtcgatgtcc
     1921 aggatgcccg agtcgaacat ctcgtagatg ccgcacttgc cgtcccagag gatgccggtc
     1981 tcgttctccg ccagaaaatc ccggcgaaaa acatagatgc tggcgttcag gtcatagatc
     2041 gccggcgcct gctggcgggc ggtatagggg ctctcgatca ccttttccac atggtcgccc
     2101 accaccttga ccatgttgaa ataggggttg cgccgcgcct gggccacgct gaagaccagg
     2161 tccaggtccg cccgggcctc ggcggcggca aaagccccgg ccacatcgct ctcccgccgg
     2221 atggggctgg tgatgtccag gtcgatcaga tggtcgtagc gcccgccgcc ccggcgctcc
     2281 atctcggcca gcgtctgctg aaagaccgcc atcttgggca ccgtgtcgcc gcacagctcc
     2341 tgcgggcgct cgaccaggac ggtctcgggg tagcgctgcg ccaccagggc ctgcagctcg
     2401 ggcgagtcgg tgttcagcgc cac
//
LOCUS       DBMDYA010000459         2420 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9319_length_2420_cov_1.177663, whole genome shotgun sequence.
ACCESSION   DBMDYA010000459 DBMDYA010000000
VERSION     DBMDYA010000459.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9319_length_2420_cov_1.177663"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="ACZPFK_10560"
     CDS             complement(<1..523)
                     /locus_tag="ACZPFK_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR1406797.1"
                     /translation="MGKSVYSVVLSDQVVAAIDKLAYAEGTSRSKLIDRVLAQYAGCD
                     TPGQRMTDIFSCIEQMAGLEEQFRLLINPSDELLQLKSAVPYKYNPTVKYAVELYEND
                     PTALGELRVSMRTQSRPLLAVLGRFFLLWVQLEEQSFQDGSYLSFEVADGKYTRQLRH
                     LPGASSAQLGEAIA"
     gene            1372..>2420
                     /locus_tag="ACZPFK_10565"
     CDS             1372..>2420
                     /locus_tag="ACZPFK_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406798.1"
                     /translation="MSKRSEQGLSLGGNMLWNSAGSFFYFACQYLPTILVVRMAADLN
                     APGVFNYAMNTTNAFLTIAVYGMRAYQVSDLEGRYSDREYVSSRLFTCLLALAACAAY
                     GGVLRLPGQTLAALLLYMLFRVGEALVDVYSGIDQRADRMDVIGKSFILRGALSCLGF
                     ALGMKFTGSLLLSVGLMAAVSLGVALAYDRPWAARLGDTAGRAAAGRVRALLVECAPL
                     MAYSFLNTAVSTIPRQFLQLQHGNAATAIFGSITAPTVVLQLGATYVFTPLISLFTRR
                     WLARDRRGFGALFGRVLLAIGGIGIAGFAGVKLLGRWGLALLYSTSENGRQILANEGL
                     LTPMVAATVLTALVL"
BASE COUNT          365 a          775 c          837 g          443 t
ORIGIN      
        1 cggcgatcgc ctcgcccagc tgggcgctgg aggcgccggg cagatgccgc agctggcggg
       61 tgtacttgcc gtccgccacc tcaaaggaga gataggagcc gtcctggaac gactgctcct
      121 cgagctgcac ccacagcagg aaaaaccgcc ccagcacggc aagcagcggc cgcgactggg
      181 tgcgcatcga gacccgcagc tcgcccaggg cggtggggtc gttctcgtac agctccaccg
      241 catacttgac ggtggggttg tatttgtagg gcaccgcgct cttcagctgc agcagctcgt
      301 cgctggggtt gatgagcagc cggaactgct cctccagccc ggccatctgc tcgatacagc
      361 tgaagatatc ggtcatccgc tgcccggggg tatcgcagcc cgcgtactgg gccagcaccc
      421 ggtcgatcag cttgctgcgg ctggtgccct cggcgtaggc cagcttgtcg atggcggcca
      481 ccacctggtc gctgagcacc acgctgtaca cacttttgcc catctctgcg cctccttttt
      541 cggatgatat tattctatca ctcggtaaat aatttgtcaa tataattata taattttatt
      601 ttaaaattat tctgattttt aattttttta acaagaactg gaaggccccc ctctgctttt
      661 ccatccggct tataccagtc ttttggattt catcctcctc tatcccccga aaggcgggct
      721 tgtcccggcc tggtcggcgg cgcaagtcgt gacggtttta aaggtgacgg ttttaagggt
      781 gacgagcggg ctgcggtggc gcgtcgcggg gcgggcaagc ggcggcaagc catggcggcg
      841 cgggttgcag cagggcaagc cgtgggtggg caagcggcgg cgagacgggc cggggtgagg
      901 cgaaccgcgg cggggtgggc gaaccacaac ggggtgggcg aaccacaacg gggtgggcga
      961 accgcgccgg ggtgggcaaa ccacgccggg gtgggcaaac cacgccgggg tgggcaaacc
     1021 acgccggggt gagccgaggt tcaacggggc aagcggcgcg gctgcggagg ggcggcggaa
     1081 aaaagcgcct caccggcccg gccgggcgag gcgcgggccc cacttttatc gcagggcaag
     1141 gccgccgtga cgggcctggg cgcggccgcc gcgcaccgcg ggggaagagc aaaactggcg
     1201 gggcgggaga ttttccgccg caaatgtggt ataatttcct ttatcaacgc gcccggacag
     1261 gcgtccgcgg cgcagggctg cggcaaaggg ccgttcgccc gtcccgccgc ccgctccgcc
     1321 ccgcgcgccc cggctgcggg ggcgcgggcc gcgtcaaaag ggagtttatc catgtccaag
     1381 agatcggaac aagggctgtc gctgggcggc aatatgctgt ggaacagcgc gggctctttc
     1441 ttttactttg cctgccaata cctgcccacc atcctggtgg tgcgcatggc cgccgacctg
     1501 aacgcgccgg gcgtgttcaa ctacgcgatg aacaccacca acgccttttt gaccatcgcc
     1561 gtctacggca tgcgcgccta tcaggtgagc gacctggagg gccggtacag cgaccgggag
     1621 tacgtctcca gccgcctgtt cacctgcctg ctggccctgg ccgcctgcgc cgcctacggc
     1681 ggcgtgctgc ggctgcccgg ccagaccctg gccgccctgc tgctgtacat gctgttccgg
     1741 gtgggggagg cgctggtgga cgtctacagc ggcatcgacc agcgggccga ccgcatggac
     1801 gtgatcggca agagcttcat tttgcggggg gcgctgtcct gcctgggctt tgcgctgggc
     1861 atgaagttca ccggcagcct gctgctctcg gtggggctga tggccgctgt ctcgctgggc
     1921 gtggccctgg cctacgatcg cccctgggcc gcgcggctgg gcgacacggc gggccgtgcc
     1981 gcggcgggcc gggtgcgggc cctgctggtt gagtgcgcgc ccctgatggc ctacagtttt
     2041 ttgaacaccg ccgtctccac catcccgcgc cagtttttgc agctgcagca cggcaacgcg
     2101 gccaccgcca tcttcggcag catcaccgcg cccaccgtgg tgctgcagct gggcgcgacc
     2161 tatgttttca cgccgctgat ctcgctgttc acccgccgct ggctggcgcg ggaccggcgc
     2221 ggcttcggcg cgctcttcgg gcgggtgctg ctggccatcg gcggcatcgg catcgccggt
     2281 tttgcggggg tgaagctgct gggccgctgg gggctggcgc tgctctattc caccagcgag
     2341 aacggccggc agatcctggc caacgagggg ctgctgaccc ccatggtggc cgccacggtg
     2401 ctcaccgcct tggtgctgtt
//
LOCUS       DBMDYA010000460         2414 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9338_length_2414_cov_1.280588, whole genome shotgun sequence.
ACCESSION   DBMDYA010000460 DBMDYA010000000
VERSION     DBMDYA010000460.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_2414_cov_1.280588"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(196..927)
                     /locus_tag="ACZPFK_10570"
     CDS             complement(196..927)
                     /locus_tag="ACZPFK_10570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1406799.1"
                     /translation="MAQYRLDRLLAEAAGIPRAEARRAILKGQVALDGAVCRRPDQKA
                     QDTAALALAGRPLRWQKYVYLMLDKPQGVVSASRDGRDTTVADLVRAQYPRRELFPAG
                     RLDKDSTGFVLLTDDGAFAHDLLAPKRHVDKVYEVLLDAPVTAGMLDGFAAGVVLADG
                     EAMLPAGLKADPANPCRATVVLHQGVYHQIKRMFGVFGAGVCALRRVAIGGVPLDPAL
                     GPGGSRPLTGEELARLRAACTAAGK"
     gene            complement(1252..>1592)
                     /locus_tag="ACZPFK_10575"
     CDS             complement(1252..>1592)
                     /locus_tag="ACZPFK_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1406800.1"
                     /translation="RLRALLQQESWIIEGVYHDWVGESFERAGRIILLDPPGWRCGLR
                     VAARFLRRKLGLERGKKESLGSLVRLLCWSHRYRKNELPQIRRALRAYGDKTVVLCSK
                     REVKDYLAGC"
     assembly_gap    1593..1602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1955..>2414)
                     /locus_tag="ACZPFK_10580"
     CDS             complement(1955..>2414)
                     /locus_tag="ACZPFK_10580"
                     /inference="COORDINATES: protein motif:HMM:NF027438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HIR1406801.1"
                     /translation="KWDDSFVAFNEVIKQSGLDLSKYKGKTVEKWDFVAPNKSTETDT
                     VSAILLVYKKQVIGAYLIGRPSGEVSALTAPAASPAPSAAPTEGQPEGSVPTMTDAEQ
                     AQQQGTELTPEQLAQVSAAAQAAGLTDEQIAAAAAAIEAAAQAPDALPSE"
BASE COUNT          348 a          884 c          771 g          401 t
ORIGIN      
        1 tattttttat cgctccacga taaaaaacaa cttatcttac tggccccggc ccgggacggg
       61 ccggatggcc gaaaaatagc ctgcggctat tttttatcgc tccgcgataa aaaataactt
      121 atcttaatgg ccccggcccg ggacgggccg gatggccgaa aaatagccta tggctatttt
      181 ccccctcccc cctcttcact ttccggccgc agtgcaggcg gcgcgcaggc gggccagctc
      241 ttcgccggtg aggggacggc tgccgccggg gccgagggcg gggtccagcg gcacgccgcc
      301 gatggccacc cgccgcagcg cgcacacccc ggccccaaag acgccgaaca tgcgcttgat
      361 ctggtgatag accccctggt gcagcaccac cgtggcccgg caggggttcg ccgggtccgc
      421 tttcagcccc gcgggcagca tggcctcgcc gtcggccagc accacgccgg cggcaaagcc
      481 gtccagcatc ccggccgtca ccggcgcgtc cagcagcacc tcatagacct tgtccacgtg
      541 gcgcttcggg gccagcaggt cgtgggcgaa agccccgtcg tcggtgagca gcacaaagcc
      601 ggtggaatcc ttgtccagcc gcccggccgg aaagagctcc cgccgcgggt actgcgcccg
      661 caccaggtcc gccaccgtcg tgtcgcgccc gtcgcggctg gcgctgacca cgccctgcgg
      721 cttatccagc atgagataga catatttctg ccagcgcagc ggccgcccgg ccagcgccag
      781 cgcggcggtg tcctgcgctt tctggtccgg cctgcggcag accgcgccgt ccagcgccac
      841 ctgccctttc aagatcgccc tgcgggcctc ggcgcgcggg atgcccgccg cctcggccag
      901 cagccggtcc aaccggtatt gtgccatgga tgaccgcctc cctggggccg cctgtcgcgg
      961 ccacggggat attatagcac ggaaagtcgg ttttgggcga gggaatatct gggcggccgc
     1021 cgcgcgggtg gccggatgcc ccgggcgcgg aaagccgtcc cgccgcctgc ctcccgccca
     1081 cgccgtctct gagcaccgct tcaaacgggc gatcgggccg cagaagacca gcgcccaccc
     1141 gcttcacacc gtccgccgga gcgtttttcc ggcgggaccg cgcgggcggg ccgggcgatt
     1201 gccgccggac ggcggcgtgt ctgggccgcc cggtcccggc gcgtcggcgc ttcagcaccc
     1261 ggcgagatag tcctttacct cccgcttgct gcagaggacg accgttttat ccccgtaggc
     1321 ccgcagagcg cggcggatct gcggcagctc gtttttgcgg taccggtgcg accagcacag
     1381 cagccgcacg agagagccca gcgactcttt tttcccccgc tcaaggccca gcttgcgccg
     1441 caaaaaccgc gccgcgaccc gcaggccgca tcgccacccg ggcgggtcga gcagaatgat
     1501 ccgccctgct ctctcaaagc tctcgcccac ccagtcgtgg tagacgccct cgatgatcca
     1561 gctttcttgc tgcaaaaggg cacgcagcct gtnnnnnnnn nnagcgccgg aattgtccca
     1621 gaacagatcg tccagctccc agcgcggcac gccggtcttt tggctcagcc gccgggccag
     1681 ccaggtcttg cccgcgccgc tgccgccgat gatatggatg cgcatcgccc cgcgccctcc
     1741 ccgcgttcgc cgggccgggc ccggcgtttt ttccattata gcacagccgg cggtatgcgg
     1801 gtaattcgaa cggtgaacgg gtaattctgc cgccgccgcg cgggcaatct cgccgccgcc
     1861 gcaaagttcc cccgtggcgc gggccgggca aaaaaaatgc agcggcagaa tgccgctgca
     1921 cagcgctgtc ttggttttga gagggcgcgc ccgcctattc ggagggcagg gcgtccggcg
     1981 cctgggccgc ggcctcgatg gccgccgcgg ccgcggcgat ctgctcgtcg gtcaggcccg
     2041 ccgcctgggc cgccgcgctc acctgcgcca gctgctcggg cgtcagctcc gtcccctgct
     2101 gctgggcctg ctcggcgtcg gtcatggtgg gcaccgagcc ctccggctgc ccctcggtgg
     2161 gggcggcgct gggcgcgggc gacgccgccg gcgcggtcag cgcgctcacc tcgccgctgg
     2221 gccgcccgat cagataggcc ccgatcacct gctttttgta gaccagcaga atggcgctga
     2281 ccgtatcggt ctcggtggat ttgttcggcg cgacgaaatc ccatttttcc accgttttgc
     2341 ccttgtactt gctcaggtcc aggccgctct gcttgatgac ctcgttgaaa gccacaaagc
     2401 tgtcgtccca cttt
//
LOCUS       DBMDYA010000461         2407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9372_length_2407_cov_1.176496, whole genome shotgun sequence.
ACCESSION   DBMDYA010000461 DBMDYA010000000
VERSION     DBMDYA010000461.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9372_length_2407_cov_1.176496"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="ACZPFK_10585"
     CDS             complement(<1..118)
                     /locus_tag="ACZPFK_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406802.1"
                     /translation="MNNNFETVIGLEVHAELNTKTKIFCGCENRFGAEVNTLC"
     gene            complement(126..1574)
                     /gene="gatA"
                     /locus_tag="ACZPFK_10590"
     CDS             complement(126..1574)
                     /gene="gatA"
                     /locus_tag="ACZPFK_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR1406803.1"
                     /translation="MKKLYEYSATELSSLLRGKEVSAAEITEAALARIEAVEGRVDAF
                     LTVTADEAREAAKQVDARIAAGEELAPLAGIPVAIKDNICTKGVRTTCASRMLENFVP
                     PYDATVIERLKAQGAVLPGKVNLDEFAMGGSCENSYFKTTKNPWDTSRVPGGSSGGSA
                     AAVASCEVPLALGSDTGGSVRCPASFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPLG
                     RTVDDVALLYGAICGHDPRDATSKNAALGAVSGEVKGLRLGVPKEYFGEGVSAEVKAA
                     VYAAVERLKGLGAEVVEVSLPSTDYALAAYYIISSAEASSNLARYDGVKYGFSGNREG
                     TLNDLYLSTRSQGFGAEVKRRIMLGTYVLSSGYYDAYYKRAKLLQGVIGQEFADAFEK
                     CDAIVTPVNPTTAFKLGEKCDDPVQMYATDVCTVTVNIAGLPGVSLPCGADAAGLPIG
                     MQVIGPKFSEARLLSIAKCYETAVGGFAVKEM"
     gene            complement(1593..1862)
                     /gene="gatC"
                     /locus_tag="ACZPFK_10595"
     CDS             complement(1593..1862)
                     /gene="gatC"
                     /locus_tag="ACZPFK_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR1406804.1"
                     /translation="MEIDVRRIAKLAKLDIPEEELPRMERELASIVSMVEKLPELASK
                     ESLLDPENTMELRPDVVQPSFPRDEMLKNVPDTAAGCLMVPKVVE"
     gene            complement(1894..>2407)
                     /locus_tag="ACZPFK_10600"
     CDS             complement(1894..>2407)
                     /locus_tag="ACZPFK_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1406805.1"
                     /translation="AIEASKGNNLDRLINGLGIRNIGDTAATLLAERFSSIDGIMAAS
                     EEEIAAIDGYGGIMAESVVEFFSKPGSRDLVEKLRAAGVNMAYTGEEKTDYLAGKTIV
                     VTGTLPTLSRDEAEELIKKNGGKATGSVSKRTSYVLAGEAAGSKLTKANDLGVPVIDE
                     AAFFEMIHRN"
BASE COUNT          388 a          801 c          732 g          486 t
ORIGIN      
        1 agcaaagggt gttgacctcg gcgccgaaac ggttttcgca gccgcagaag atctttgttt
       61 tcgtattcag ctcagcgtgg acctcaaggc cgatgacggt ttcaaagttg ttgttcatct
      121 ctcgctcaca tctcctttac tgcaaagccg cccacggccg tctcatagca cttggcgatc
      181 gagagcagcc gcgcttcgct gaatttgggg ccgatgacct gcatgccgat gggcaggccc
      241 gcggcgtcgg cgccgcaggg caggctgacg ccgggcaggc cggcgatatt caccgtgacg
      301 gtgcacacgt cggtggcgta catctgcacc gggtcgtcgc atttttcacc cagcttgaaa
      361 gcggtggtgg ggttgaccgg cgtgacgatg gcgtcgcact tttcaaaagc gtcggcgaac
      421 tcctgcccga tgaccccctg cagcagcttg gcgcgcttgt agtaggcgtc gtaatagcct
      481 gagctgagca cgtaagtacc aagcatgatg cggcgcttca cctctgcgcc aaagccctgg
      541 ctgcgggtgg agagataaag gtcgttcagc gtgccctccc ggttgcctga aaagccgtat
      601 ttcaccccgt cgtaccgggc caggttgctg ctggcctcgg cggaggagat gatataatag
      661 gcggccagcg cgtagtcggt ggagggcagc gagacctcta cgacctccgc ccccagcccc
      721 ttgaggcgtt ccaccgcggc atacaccgcc gctttgacct cggccgagac gccctcgccg
      781 aagtactcct tgggcacgcc gaggcgcagg cctttcacct cgcccgacac cgcgccgagc
      841 gccgcgtttt tcgaggtggc gtccctgggg tcgtggccgc agatggcgcc gtaaagcagg
      901 gccacgtcgt ccaccgtgcg gccgagcggg ccgatctggt ccagcgagga ggcaaaggcc
      961 accaggccat aacggctgac cgcgccgtag gtgggcttca gccccacgat gccgcagaag
     1021 ctggccgggc agcgcaccga gccgccggtg tccgagccca gggccagcgg cacctcgcag
     1081 gaggcaaccg ccgccgcgct gccgccggag gagccgcccg gcacccggct ggtgtcccag
     1141 gggttcttcg tggttttgaa ataagagttc tcgcagctgc cgcccatggc gaactcgtcc
     1201 agattcacct tgccgggcag cacggcgccc tgggctttca accgctctat gacggtggcg
     1261 tcataggggg gcacaaaatt ttccagcatg cgggaggcgc aggtggtccg cacccccttg
     1321 gtgcagatgt tgtccttgat ggccacgggg atgcctgcca gcggagccag ctcctcccct
     1381 gccgcgatcc tcgcatccac ctgtttggcg gcctcgcgcg cctcgtcggc tgtgacggtc
     1441 aaaaaggcgt ccacccggcc ctccacggcc tcgatgcgcg caagcgccgc ctcggtgatc
     1501 tcggcggcgc tgacctcctt gccgcgcagc agggaggaga gctcggtggc cgaatactca
     1561 tacagctttt tcatctttct tcctccctct ttttactcta cgaccttggg caccatcagg
     1621 cagccagccg cggtgtcggg cacgtttttg agcatttcgt cccgcgggaa agacggctgc
     1681 accacgtccg ggcgcagctc catcgtattc tccgggtcga gcaggctctc cttgctggcc
     1741 agctcgggaa gcttttccac catgctcacg atactggcaa gctcccgctc catgcggggg
     1801 agctcctcct ccgggatgtc gagcttcgcg agcttggcaa tgcgtcgcac atcgatctcc
     1861 atactggaca ctcctttttt attctttcta ttttcaattc ctgtgaatca tctcaaaaaa
     1921 cgccgcctcg tcgatgaccg gtacccccag gtcgttcgcc ttggtcagct tgctgcccgc
     1981 ggcctcgccc gccagcacat agctggtgcg ctttgaaacc gagccggtgg ccttgccgcc
     2041 gttctttttg atcagctcct ccgcctcgtc gcgggagagg gtgggcaggg tgccggtgac
     2101 cacgatggtc ttgcccgcca gataatcggt cttttcctcg ccggtatacg ccatgttgac
     2161 cccggccgcg cgcagctttt ccaccagatc gcggctgccc ggctttgaga aaaactcgac
     2221 cacactctcg gccataatgc cgccgtagcc gtcgatggcg gcgatctcct cctcgctcgc
     2281 ggccatgatg ccgtcgatgc tcgaaaagcg ctcagccagc agggttgccg ctgtatcgcc
     2341 gatattgcgg atgcccaggc cgttgatcag cctgtccagg ttgttgccct tagacgcctc
     2401 gatggcg
//
LOCUS       DBMDYA010000462         2406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9378_length_2406_cov_1.438612, whole genome shotgun sequence.
ACCESSION   DBMDYA010000462 DBMDYA010000000
VERSION     DBMDYA010000462.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9378_length_2406_cov_1.438612"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="ACZPFK_10605"
     CDS             complement(<1..313)
                     /locus_tag="ACZPFK_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406806.1"
                     /translation="MRDIGKNIKDLREQKGLTQEALAEQLFVTRQTVSNYETGRTRPD
                     IEILMRIAGALDSPVDAVLYGPPVPQSRRHARRRMAAGGGLLALTAASAALLHPIAAQ
                     VA"
     gene            444..653
                     /locus_tag="ACZPFK_10610"
     CDS             444..653
                     /locus_tag="ACZPFK_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406807.1"
                     /translation="MDDSREAEASPVYNTDSNSLTLSDGREGEPEAPGKRKLSTGKDP
                     LRKKNALKGKRGLSGRKDVPRGKTL"
     gene            815..2230
                     /locus_tag="ACZPFK_10615"
     CDS             815..2230
                     /locus_tag="ACZPFK_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406808.1"
                     /translation="MDAAERQDAKYRQMIETPIPRLVCTLAVPTIISMLVTSIYNMAD
                     TFFVGKIGTSATGAVGVVFSLMAIFQAVGFTIGIGSGNLVSRLLGSRKRQEAEHVACT
                     GFYTAFAAGVVLMVLGLVFIDPLMRVLGATETILPYARAYARYILLGAPFITSSFVLN
                     NLLRFQGSAMYAMVGITAGGVLNILLDPLFIFVFGLETAGAAIATVLSQIVSFSILLI
                     QCWRGGNIRMNPKKVRLKWDIYHEVLRGGLPSFYRQVLASIATICLNLAAGPFGDAAI
                     AAMSIVSRVMMFAQSALIGFGQGFQPVCGFNYGAGRYDRVRKAFWFCVKVAGVGLLLI
                     AVAGECFAPQVIGLFRKGDAAVTTIGTAALRLQCLVFPLMGWSICLNMLQQNIGKSKA
                     ASILSAARQGLFFLPLILTLPRFLGVLGIQLSQPLSDLCTFALLVPMGISLLRELREK
                     DEQSRAARADEQGRAPGEGAD"
BASE COUNT          414 a          768 c          796 g          428 t
ORIGIN      
        1 ccgccacctg ggcggcgatg gggtggagga gcgcggccga ggctgccgtc agagccaaca
       61 ggccgcctcc tgccgccatg cgccggcggg cgtggcggcg gctctgcggc accggcgggc
      121 cgtacagcac cgcgtccacg gggctgtcca gcgcccccgc aatccgcatc agtatctcga
      181 tatccggccg ggtgcgcccg gtctcgtagt tcgacacggt ctgccgcgtc acgaacagct
      241 gttccgcgag cgcctcctgg gtcaggccct tttgctcgcg caggtctttg atgttttttc
      301 cgatgtcccg cacagccatc acctccctta aagcatagcg ccgggaggtg gaaatgtcac
      361 gcaaggattc cttgcgcggc cggcggaagc agaggtgcgg gccgcaaaca gacaattcgg
      421 agccgcggcg aaaaaagcgc cccttggacg actcgcggga ggcggaggcc tcgcccgtat
      481 acaatacgga ctccaacagc ctgacccttt cagatggccg ggagggagag ccggaagccc
      541 cggggaaaag aaagctctcg acaggaaagg acccgctcag gaagaaaaac gcgctcaagg
      601 ggaaaagggg gctctcgggg agaaaggatg ttcccagggg aaagacgctc tgaaagggga
      661 aagccgcctc ctgccctgcg gcaggcggag taagaaaagc agccaaatag ttgcacgcac
      721 aactaatttg cgctagaatg aaaaggacaa ttacagaagc gcccgtccgg cgcgccggag
      781 cgcgcggcgg cgggaaacag aaaggaagaa acgaatggac gcagcggaac gtcaggacgc
      841 aaaatacaga cagatgatcg agacccccat cccaaggctt gtctgcacgc tggcggtgcc
      901 caccatcatc agcatgctgg tcacctccat ctacaacatg gccgacacct ttttcgtcgg
      961 caagatcggc acctcggcca ccggcgcggt gggggtggtc ttttcgctga tggcgatctt
     1021 ccaggcggtg ggcttcacca tcggcatcgg cagcggcaac cttgtgtcgc ggctgctggg
     1081 cagccgcaag cggcaggagg cggagcacgt ggcctgcacc ggcttttaca ccgcctttgc
     1141 ggcgggcgtc gtgctgatgg tactggggct tgtctttatc gacccgctga tgcgggtgct
     1201 gggcgccacc gagaccattc tgccctatgc ccgcgcctat gcgcgctata tcctgctggg
     1261 cgcgcccttt atcaccagct cgtttgtgct caacaacctg ctgcgctttc agggcagcgc
     1321 catgtacgcc atggtgggca tcaccgccgg cggggtgctg aatatcctgc tggaccccct
     1381 cttcatcttt gtgtttgggc tggagacggc gggcgccgcc atcgccaccg tgctcagcca
     1441 gatcgtcagc ttttccatcc tgctgatcca gtgctggcgc gggggcaaca tccgcatgaa
     1501 ccctaaaaag gtgcggctca aatgggatat ctatcacgag gtgctgcgcg gcggcctgcc
     1561 ctccttttac cggcaggtgc tggccagcat cgccaccatc tgcctcaacc tggcggccgg
     1621 gccgttcggc gacgcggcca tcgcggccat gtccatcgtc tcccgcgtga tgatgttcgc
     1681 ccagtcggcc ctgatcggct ttgggcaggg ctttcagccg gtgtgcggct tcaactacgg
     1741 cgcgggccgg tatgaccggg tgcgcaaggc cttttggttc tgcgtcaagg tggccggcgt
     1801 cggcctgctg ctgatcgcgg tggcgggcga gtgctttgcg ccgcaggtga tcgggctgtt
     1861 ccgcaagggg gacgcggcgg ttaccaccat cggcaccgcg gccttgcggc tgcagtgtct
     1921 cgtcttcccg ctgatgggct ggagcatctg cctgaacatg ctgcagcaga acatcggcaa
     1981 gagcaaggcc gcgtcgatcc tgtcggcggc gcggcagggc ctgttctttt tgccgctgat
     2041 cctgacgctg ccccggtttt tgggggtgct gggcatccag ctcagccagc ccctgagcga
     2101 cctttgcacc tttgcgctgt tggtgcccat ggggatatcg ctgctgcgcg agttgcggga
     2161 aaaagacgag caaagccgcg ccgcgcgtgc ggacgagcaa ggccgcgcgc cgggggaggg
     2221 ggccgactga tgcccgagca gttctacgcc aaccgttacg cctccatcct ctaccgcttt
     2281 ggacagcggt ttttggcccg cgccctgcgg gaggaggctc tgccgctgga acccgcccag
     2341 ctgcccgtct ttctgcgcgc ggcacaccgg ccgggcatcg cgcaggagga catcgccgcc
     2401 gccacc
//
LOCUS       DBMDYA010000463         2406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9379_length_2406_cov_1.344902, whole genome shotgun sequence.
ACCESSION   DBMDYA010000463 DBMDYA010000000
VERSION     DBMDYA010000463.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9379_length_2406_cov_1.344902"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            227..1564
                     /locus_tag="ACZPFK_10620"
     CDS             227..1564
                     /locus_tag="ACZPFK_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1406809.1"
                     /translation="MEQRSLFDDREWTNPLASRLRPETLDDFVGQRHLLGEGKVLRQL
                     IDRDEIPSMIFWGPPGVGKTTLARIIARRTQARFIDFSAVTSGIKEIKKVMDEAEANR
                     RMGERTILFVDEIHRFNKAQQDAFLPFVEKGSIVLIGATTENPSFEINSALLSRCKVF
                     VLQALAPEDLAALLRRAVTDPHGFGSRPVEIGDDELSMIANFAGGDARTALNTLEMAV
                     LNGELEDGRTRVTKEIIEQCVSKKSLLYDKNGEEHYNLISALHKSMRNSDADAAVYWL
                     ARMLEAGEDPLYVARRVVRFASEDVGMADSRALEVAVAAYQACHFIGMPECSVHLTHA
                     VVFLSLAPKSNALYTAYESAKRDAQKMLAEPVPLQIRNAPTRLMKDLHYGEGYIYAHD
                     TEEKLSAMQCLPDSVQGRTYYRPTEEGAEARVKQRKEQIEVWKREQREKQKKG"
     gene            1965..>2406
                     /locus_tag="ACZPFK_10625"
     CDS             1965..>2406
                     /locus_tag="ACZPFK_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1406810.1"
                     /translation="MKKTLACIVALALAAGMLAACGQGGSSVSTPPQGGAGTGSSAAN
                     GKDYHFIYVTPAGANAYWVDVQDGIDAAARDLGVTVETVGPDDVDQIKQIEAMEAAAV
                     DKPDGIMTMALNPESFTDPVDKIVEGGVPVVLLDGDAPESGRSFY"
BASE COUNT          526 a          682 c          763 g          435 t
ORIGIN      
        1 ttgcggagca ataaaaaata gctgcaggct gttttttcgg ccatccggcc agtcctctgg
       61 ccggggctat caagataaat tgttttttgt tgcggagcaa taaaaaatag ccgcaggcta
      121 ttttttcggc catccggcca gtcctctggc cggggccatc aagaaaaagc cgcccttcgg
      181 ccgggcatgc cccggctttg agggactggg agggaggggg acagagatgg aacagcgctc
      241 actgtttgac gaccgggagt ggaccaaccc cctggccagc aggctgcggc cggagacgct
      301 ggacgatttt gtgggccagc gccatctgct gggcgagggc aaggtgctgc gccagctcat
      361 cgaccgggac gagatcccgt ccatgatttt ttgggggccg cccggcgtgg gcaagaccac
      421 cttggcccgc atcatcgcac ggcgcaccca ggcgcgcttt attgattttt ccgcggtgac
      481 cagcggcatc aaagagatca aaaaggtgat ggatgaggcg gaggccaacc gccgcatggg
      541 ggagaggacc atcctttttg tggacgagat ccaccgcttc aacaaggccc agcaagacgc
      601 ctttctcccc tttgtggaaa aaggcagcat cgtcctgatc ggcgccacca ccgaaaaccc
      661 ctcctttgag atcaactcgg cgctgctctc gcgctgcaag gtgtttgttc tgcaggccct
      721 cgcgccggag gacttggccg ccctgctgcg gcgggccgtc accgacccgc acggcttcgg
      781 cagccggccg gtggagattg gggacgacga gctgtcgatg atcgcaaact ttgccggcgg
      841 cgacgcgcgc accgccttga acacgctgga gatggccgtg ctcaacgggg agcttgagga
      901 cgggcgcacc cgcgtcacca aagagatcat cgagcagtgc gtgagcaaaa aatcgcttct
      961 ctacgacaaa aacggggaag agcattacaa cttgatttcg gcgctgcaca aatcgatgcg
     1021 caacagcgac gccgacgcgg cggtctattg gctggcgcgg atgctggagg cgggggagga
     1081 cccgctctat gtggcgcggc gggtggtgcg ctttgccagc gaggatgtgg gcatggcgga
     1141 cagccgggcg ctggaggtgg cggtggcggc ctatcaggcc tgccatttta tcggcatgcc
     1201 cgagtgcagc gtccatctca cccacgcggt ggtgtttttg tccctggccc ccaagtcgaa
     1261 cgcactctat acggcctacg agagcgccaa gcgggacgcg cagaaaatgc tggccgagcc
     1321 ggtgcccctg cagatccgca acgcgcccac acggctgatg aaggacctgc attacggcga
     1381 gggctatatc tacgcccacg acactgaaga aaagctcagc gccatgcagt gcctgcccga
     1441 ttccgtgcag gggcggacct attaccgccc caccgaggag ggggccgagg caagggtcaa
     1501 acagcgcaaa gagcagatcg aggtctggaa gcgggaacag agggaaaaac agaaaaaagg
     1561 gtgaggccct cttcagcccc ccgcctttgt ccggcgggtg cggcgacgct ctgcgcgcaa
     1621 atcccccgcc gggaaagacg cttttcagaa aaggctcaag aggaacattt cataaccaaa
     1681 aacaagaccg gcgcacagcg catgccacgc tgtgcgccgg tcttgtttaa aacagaaaac
     1741 ggtttcgttc gctcttgaaa agggacaaca gaaaccggtt tagataaaac tctgaaaatc
     1801 agctttgttt tttgactatt attgcccttg ttttgtcgaa aaggaagctt tatactttga
     1861 ttgtggagta aaacttcatt aaaacaaaat atttggagta aatatcccgc aacaagcgtt
     1921 tgcgctgcac tctcgcagcg tagcaataaa aggaggaaat aaaaatgaaa aagaccttgg
     1981 catgtatcgt cgcactcgct ttggcggccg gtatgctggc agcctgcggg cagggcggtt
     2041 cttccgtctc cacgcccccg cagggcggcg ccggcacggg cagctccgcg gcgaatggaa
     2101 aggattatca ttttatctat gtgacccccg cgggagccaa cgcctactgg gtggatgtgc
     2161 aggacggcat cgacgcggcg gcgcgggacc tgggggtgac cgtggagacc gtcgggccgg
     2221 acgatgtgga ccagatcaag cagatcgagg ccatggaggc ggccgccgtc gacaagccgg
     2281 acgggatcat gaccatggcg ctgaacccgg aatcattcac cgaccccgtc gacaagatcg
     2341 tggagggcgg cgtgcccgtg gtcctgctgg acggcgacgc gccggagagc ggtcgctctt
     2401 tctatg
//
LOCUS       DBMDYA010000464         2404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9387_length_2404_cov_1.491099, whole genome shotgun sequence.
ACCESSION   DBMDYA010000464 DBMDYA010000000
VERSION     DBMDYA010000464.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9387_length_2404_cov_1.491099"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="ACZPFK_10630"
     CDS             <1..585
                     /locus_tag="ACZPFK_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1406811.1"
                     /translation="SDEIWSDIVLNGNRHIPTQSVSEDAKKRTIAIYAPSKTFNLAGL
                     VGSYHIVYDEYLRDRLNASTGKSGYNHMNVLSMHALIGGYGEEGEAWVDELCDVLSAN
                     VNYAYEYIRAKFDGVSLFKPQGTYMMYLNCEGWCKAHGKSMDELLQAGASVGVIWQDG
                     RPFRHPYGIRINLALPQSLLREALERLDRYVFNA"
     gene            828..1190
                     /locus_tag="ACZPFK_10635"
     CDS             828..1190
                     /locus_tag="ACZPFK_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024088.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HIR1406812.1"
                     /translation="MKKRSLPIRPWMGLLGLLGFLGFLPKYGGGHNYLFFLFFAFFSW
                     FFWGLLGAEKADERLLANQARAMKTIGVLFALLCFSLIFLLDRGVAADTVLLVGALGY
                     AACFIAAPALVWYLDRRG"
     gene            1195..1395
                     /locus_tag="ACZPFK_10640"
     CDS             1195..1395
                     /locus_tag="ACZPFK_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1406813.1"
                     /translation="MGGFVSYLKKYRLLAGLTQGELAELVDVRRETIVRLESGRYNPS
                     LKLAIDIARAVQAPIEEIFVFE"
     gene            complement(1803..>2404)
                     /locus_tag="ACZPFK_10645"
     CDS             complement(1803..>2404)
                     /locus_tag="ACZPFK_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406814.1"
                     /translation="YVEKVIGGVLYRMTECDNVEIFADRGLYLCVQQGSFYNVDAYLW
                     DQATGTLTPNPAYKGLNLLFDLPLDGSLADPQKAQAYLDGMWKEPSQEAPQTEDDPLA
                     QTIAAYQDKSAEELIAAGTLAEDFSRTLRRDENGDYLGAGDMMPTVLAEQVAGCQTGD
                     CWVYSLGASSAADGVVRAQATIATFNGDGTITLATYFLD"
BASE COUNT          476 a          678 c          770 g          480 t
ORIGIN      
        1 tctgacgaga tctggtccga catcgtgctc aacggcaacc gccatatccc aacccagtcc
       61 gtctcagagg atgccaaaaa gcgcacgatc gccatctatg ccccctccaa aacctttaat
      121 ctggccggcc tggtggggtc ttatcacatc gtctatgacg agtacctcag agaccggttg
      181 aacgcctcca ccggcaaatc gggctacaac catatgaacg tcctctcgat gcacgccttg
      241 atcggcggct acggcgagga gggagaagct tgggtggacg agctctgcga cgtgctcagc
      301 gccaatgtga actatgccta tgagtatatc cgggcgaagt tcgatggggt gagcctcttc
      361 aaaccgcagg gcacctatat gatgtatctg aactgcgagg gctggtgcaa ggcacacggc
      421 aagagcatgg acgagctgct gcaggccggg gcctcggtgg gggtgatctg gcaggacggc
      481 aggcctttcc gccaccccta cggcatacgg atcaacctgg cgctgcccca aagcctcctg
      541 cgggaggcgc tggaaagact ggaccgctac gtcttcaacg cctgagaggc ccattacggc
      601 gctgccgccg cagaaaaata gaaaaaagga cagccagccg ccctatttta aagggcggcc
      661 agctgtcctt tttgatgctg cgccgtggca gcggagcggg caccccccca gggcccctga
      721 gaaaaaacag caaaacacct tgacgagaga aagaaaaagt gatattttta tcactaaaag
      781 atataaatat atcaccatat ccgcgccttg acgcggggga ggttgctatg aaaaagagat
      841 cgcttccgat ccgtccctgg atggggcttc tggggctgtt gggctttctg ggattcctgc
      901 cgaaatacgg gggcgggcac aactatctgt tctttctttt ctttgccttt ttttcctggt
      961 ttttctgggg cctcctcggg gcggaaaagg cggacgagcg gctgctggca aatcaggccc
     1021 gggccatgaa gaccatcggc gtgctgtttg cgctgctctg cttttcgctg atttttctgc
     1081 tggatcgcgg cgtcgctgcg gacaccgtgc tgctggtggg ggcgctggga tatgcggcct
     1141 gcttcatcgc cgccccggct ctggtctggt atctggaccg cagggggtga ggggatgggg
     1201 ggctttgtca gctatctcaa aaaatatcgg ctgctggcgg ggctgacgca gggggaactg
     1261 gcggaactgg tggacgtgcg acgggagacg atcgtgcggt tggaatcagg gcgctacaac
     1321 ccctcgctca agctggccat cgacattgcc cgcgcggtgc aggcgccgat cgaggagatc
     1381 tttgtgtttg aataaacggg gacaaaaggc gcctttcggg cagcccggca tcccacgtcc
     1441 gggcccgggc ctctggctct aaaggccgag aggctgtgga aagagggggg cggatagggg
     1501 acgatccggt tttggcggga gaaagacttc ctgcaaaaga gcgctctccc gcagggagcc
     1561 gtcgaagccg ccggacagct acaaagggcc tgcggtcgcc cgggaaatgc cggggaggac
     1621 tgtggggagg cggccggtcc ctggcggcag gcgcaagctt ttccgcgctt gccgcacaag
     1681 ctctttttcc gggggctgcg gcagaccgca agaggcgact gagaaaagaa tgaaagcgca
     1741 aaagagcttg acgatctttg cggatcgtca agctcttttg atgcgctcga gattggatag
     1801 ggtcagtcca gaaaataggt tgccagggta atggtgccgt cgccattgaa tgtggcgatg
     1861 gtggcctggg cccggacaac gccgtctgcg gcgctgctgg cgcccagact gtacacccag
     1921 cagtcgcccg tctggcagcc ggcgacctgc tccgccaaca ctgtgggcat catgtcgccc
     1981 gctcccagat aatcgccgtt ttcatcgcgc cgcagggtac ggctgaagtc ctccgccagg
     2041 gtgccggcgg caatcagctc ttcggcgctc ttgtcctgat acgcggcgat cgtctgtgca
     2101 agggggtcgt cttccgtctg cggggcctct tgcgacggct ctttccacat gccgtcaagg
     2161 taggcctggg ctttttgcgg gtcagccagg ctgccgtcca gcggcaggtc aaacaacagg
     2221 ttcaggccct tgtaggcggg gttgggggtg agagtgccgg tggcttggtc ccacagatag
     2281 gcgtccacgt tgtagaacga tccctgctgc acacagagat aaaggccgcg atcagcgaaa
     2341 atctccacat tgtcgcattc cgtcatgcgg tacagcacgc cgccgatcac cttttccaca
     2401 taag
//
LOCUS       DBMDYA010000465         2399 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9414_length_2399_cov_1.488686, whole genome shotgun sequence.
ACCESSION   DBMDYA010000465 DBMDYA010000000
VERSION     DBMDYA010000465.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9414_length_2399_cov_1.488686"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="ACZPFK_10650"
     CDS             <1..249
                     /locus_tag="ACZPFK_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1406815.1"
                     /translation="TEHMDQLHKVAEALLERETLSGEEFKAIMEGRELPPPKEEQSKK
                     DAADGQKSPVDAAEPRHEQAEESGAPMPPPLTQNEENQ"
     gene            complement(875..2101)
                     /locus_tag="ACZPFK_10655"
     CDS             complement(875..2101)
                     /locus_tag="ACZPFK_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1406816.1"
                     /translation="MCSFYVLVCNISHLLLQLNDRSDFMTDREAEIYKIIRDNPMISQ
                     NEIAQRLGITRSAVSVYLNNMTKKGILRGRGYLLNDAVYPVIIGPGHIDILSHCEEGQ
                     TTAGTRGTGFYCRSATVAYGGAVKNVANYLARLECHPRAIFSVGADFFGTHFLRDCRE
                     NGINVDGSLVVPDGVTPIYNEISTSSGRVASAYMKDNLADRLTASFLHTKQRLLKGAN
                     EIVFHDSLSFSAGEYITSACAEAYTIFFSVMYPDTVKFKDILSRFKLVVLPIDILLQI
                     ALPEAAVTADTYSVSDLKEAFSRMREMGVTNLMISCTPSQICYVQKDMFFLHNSATPA
                     AFRNKEVRTHRDAFVAGIVYCHENDVPVDKMLDFLAASKLVAMGSDNFFETHYCLSLV
                     EEKVRELNSELKLFRY"
BASE COUNT          620 a          584 c          661 g          534 t
ORIGIN      
        1 accgagcaca tggatcagct gcacaaggtg gcggaggcgc tgctggagcg ggagaccctc
       61 tccggcgagg agttcaaggc catcatggag ggccgcgagc tgccgccgcc caaggaggag
      121 cagagcaaaa aggacgccgc agacggacaa aaatcaccgg tagatgcggc agagcctcgg
      181 cacgaacagg cggaggagag cggagcccct atgcccccgc ctctgacgca gaacgaggaa
      241 aatcaataga aaagcggcgg ctaggcgctt tgaggtaagt gaacgtcggc attgagccga
      301 tggactggca cccgttatat aaaaaaaggt tggcaatctg cagagattgc caaccttttt
      361 tattgtctgc ctcataaaaa aagccggttc aaactgttgt aggccctcag cagggaaagg
      421 ggcaatacag aaaaattcgc gctgcttgcc tgcggcgatg cttgcttgcc tactcgcgca
      481 ggcatatcgc aggatgaaac cgcaaccccc tctgtggctg cggggcggat ttgagggcac
      541 tccgcgcaca cgtcttcctt ttatagggga tgttttttgt tgaaacacac tcttctgcgt
      601 gttctttttg tgcgcgcttg cgctgagtgt cggcaatcaa ggagtgaaaa ccgtgttcct
      661 ctgctggttg catggtttct ttttacagtt ttgtgatacg gaacaacccg agattccctc
      721 cggccgtcga aaaaagcgca aaagggccta tcccagggga aaggccccgg atgggatgat
      781 gggcgaatcc cccacccgct gtgctcattc tgtgcaggaa tcttaaaagc aaacagcctc
      841 cggtttggcc gggggctgtt tgctttttta gaagtcaata acgaaagagt ttgagctcgc
      901 tgtttaattc cctgactttt tcttctacaa gagagagaca ataatgcgtt tcaaagaaat
      961 tgtccgaccc catggccacc aactttgaag cggccaaaaa atccagcatt ttgtccaccg
     1021 ggacgtcgtt ttcgtggcag tatacgatcc ccgccacaaa tgcgtcgcga tgggtgcgca
     1081 cttccttatt gcgaaaggcg gccggcgttg cggagttgtg cagaaagaac atgtcctttt
     1141 gcacgtaaca gatctgagac ggcgtgcagg agatcatcag attggtgacc cccatctcgc
     1201 gcatgcggga aaaagcttcc tttaggtcgc tgacggaata ggtgtcggcg gtcacggcgg
     1261 cctccggcag ggcgatctgc aacaggatat cgatggggag caccaccagc ttgaagcggc
     1321 tcaggatgtc cttgaatttg accgtgtcgg gatacatgac ggagaaaaag atggtatagg
     1381 cctccgcgca ggccgaggtg atatattcgc cggccgaaaa agagagggag tcgtgaaaga
     1441 cgatctcatt tgcgcctttc aacaagcgct gcttggtgtg taaaaaagag gcggtcagcc
     1501 ggtccgccag gttgtctttc atataggccg aggcaaccct gccgctggag gtggagatct
     1561 cgttgtagat gggggtgacc ccgtccggca ctaccaggga accgtccaca ttgatcccgt
     1621 tttcacggca gtcgcgcaga aaatgggtgc cgaaaaaatc agcgccaacc gagaaaatag
     1681 cccggggatg acattccaga cgcgccagat agttggcgac atttttgacg gcgccgccgt
     1741 aggcgacggt ggcgctgcgg cagtaaaacc ctgtgcctct tgtgccggcg gtagtttgtc
     1801 cctcctcaca atggctgagg atatcaatgt gtccggggcc gataatcaca gggtagaccg
     1861 catcgttcag cagatatccc ctaccgcgca aaatgccttt ctttgtcata ttgttgagat
     1921 agacagagac cgccgaacgg gtgatgccca gccgctgtgc gatttcattt tgcgagatca
     1981 tcgggttatc ccggatgatt ttatagattt cagcttctcg atccgtcata aaatcactcc
     2041 tgtcgttcaa ttgtaacagt aaatgggaaa tattgcaaac taaaacataa aaagaacaaa
     2101 ttgtgaatta tacataaaaa atatataaaa tttgttgata attacaattg aaaaatagat
     2161 tgatcggcag atataatata gcaaaagcaa gtgtaatttg tcaaacgctg tataagttta
     2221 acaaaaatca gaaaaaagca gcaaaagcaa acgcttgcgt tcggcccggc ggccattctg
     2281 ccgctgcaac ccctattcga aggaggaaaa aatgaagaag attttatctc ttgttttggc
     2341 gctttccctg atggtcacgg cgactgcctg cggaggcaaa agcgcaagca cttcccaga
//
LOCUS       DBMDYA010000466         2398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9417_length_2398_cov_1.843709, whole genome shotgun sequence.
ACCESSION   DBMDYA010000466 DBMDYA010000000
VERSION     DBMDYA010000466.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9417_length_2398_cov_1.843709"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="cas1"
                     /locus_tag="ACZPFK_10660"
     CDS             complement(<1..762)
                     /gene="cas1"
                     /locus_tag="ACZPFK_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR1406817.1"
                     /translation="MRKLLNTLFVLSEDSYLALEGETVLVLAEEEKRGQFPLHTLESI
                     LSFSYKGASPALMGACAARGVNLCFFTPRGRFLARCCGEDRGNVLLRRTQYRAADDER
                     QSCLAARSFIFGKVYNGRWCLERTARGHSMRADAEALKNASRQMAQLLPRILETEDPD
                     ELRGLEGQAATAYFSVFDEMILNQKDSFFFSGRSRRPPLDNMNALLSFAYTLLASDCA
                     AALEGAGLDSYVGFLHRDRPGRVSLALDLMEELRSV"
     gene            complement(759..1415)
                     /gene="cas4"
                     /locus_tag="ACZPFK_10665"
     CDS             complement(759..1415)
                     /gene="cas4"
                     /locus_tag="ACZPFK_10665"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR1406818.1"
                     /translation="MDDGDFLMLSELQHFCFCHRQWALIHIEQQWSENLRTVDGQLFH
                     RRAHDEQQTERRGDLLITRGLRVVSHRLGVSGICDVVEFHRAEPGITLFGQEGGWQPY
                     PVEYKKGEPNAFGADELQLCGQALCLEEMLACEIPEGSLFYGETRRRKRVPFDAALRE
                     KVEGMLGEMRALYARGHTPRVKPSKSCNACSLKEICLPRLQSVPAASGYLKAHLEEAT
                     "
     gene            complement(1418..2308)
                     /gene="cas7c"
                     /locus_tag="ACZPFK_10670"
     CDS             complement(1418..2308)
                     /gene="cas7c"
                     /locus_tag="ACZPFK_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HIR1406819.1"
                     /translation="MAEAIKNRYEFVVLFDVENGNPNGDPDAGNMPRLDPETGYGIVT
                     DVCLKRKIRNYVETVKEGEAGYRIYIKDGVPLNRSDAEAYAYLGTDDKKVKEDKKKDP
                     ELDCKIRDFMCQNFYDIRTFGAVMTTFVKAALNCGQVRGPVQLGFARSIDPLIPQEIT
                     ITRVAITTETDAERKGTEMGRKYIIPYGLYRAEGFISANLARKTTGFSEEDLALFWQA
                     VINMFENDHSAARGKMAVRELIVFKHDSELGNAPSYKLFDTVKVTRKKEVASARSYQD
                     YEVAVDTQALPEGVSCTRMI"
BASE COUNT          435 a          787 c          682 g          494 t
ORIGIN      
        1 cacactccgc agttcttcca tcaggtccag ggcgagcgac accctgccgg ggcgatcccg
       61 atgcaaaaag cccacgtaag aatccaagcc agcaccctcc agcgccgccg cgcagtcgga
      121 tgcgagcagc gtataggcaa aggagagaag cgcgttcatg ttgtccaggg gtggcctccg
      181 gctgcgcccg ctaaaaaaga aactgtcttt ctggttcaaa atcatctcat cgaacacact
      241 gaaataagcc gtggcggcct gtccctccag cccccgcagc tcgtccgggt cctctgtctc
      301 caaaatgcgc ggcagcagct gagccatctg ccgggaggcg tttttcagcg cttcggcatc
      361 cgcgcgcatg ctgtgacccc gggccgtgcg ctccaaacac cagcggccgt tgtagacctt
      421 gccgaaaata aagctgcgcg cggcaaggca gctttgccgt tcatcatccg ccgcccggta
      481 ctgcgtcctg cgcagcagca cgttgccccg gtcctccccg cagcagcgcg ccaaaaagcg
      541 gccccgcggc gtaaaaaagc aaaggtttac gccgcgtgcc gcacaggcgc ccatcagcgc
      601 cgggctggcc cccttataag aaaagctgag aatgctttcc agcgtgtgca gcgggaactg
      661 cccgcgcttt tcctcctctg cgaggacgag aacagtctcc ccctccagcg ccagataact
      721 gtcctctgag agcacaaaca gggtgttgag cagctttctc atgtcgcctc ctccagatgc
      781 gctttcagat acccggacgc ggcgggcacg ctctgcagcc ggggcaggca gatctctttc
      841 agcgaacagg cgttgcagga cttggacggc ttgacccgcg gggtgtgccc gcgcgcatag
      901 agggcgcgca tctcccccag catgccctcg actttctccc gcagcgccgc atcaaagggc
      961 acccgctttc tgcgccgcgt ctcgccgtaa aacaagctgc cctcggggat ctcacaggcc
     1021 agcatctcct ctaggcagag cgcctgcccg cagagctgca gctcgtcggc gccaaaggca
     1081 ttgggctccc cttttttgta ctccacagga tagggctgcc aaccgccctc ctggccaaag
     1141 agggtgatgc cgggctctgc gcggtggaac tcgaccacat cgcagatgcc cgacaccccc
     1201 agccggtggg agacaacgcg caggccccgc gtgatgagca ggtctccgcg ccgctcggtc
     1261 tgctgctcgt cgtgggcgcg ccggtgaaag agctgcccgt ccactgtgcg caggttctcc
     1321 gaccactgct gctcgatatg gatcagcgcc cactgcctgt ggcaaaagca aaaatgctgc
     1381 agctcagaca gcatcaaaaa atcgccgtcg tccatcctca gatcatccgg gtgcaggaca
     1441 cgccctccgg cagggcctgc gtatccaccg ccacctcata atcctgatag ctgcgcgcgc
     1501 ttgccacctc ttttttgcgc gtcaccttta cggtgtcaaa gagcttgtag gacggcgcgt
     1561 tccccagctc gctgtcgtgc ttgaagacga tcagctcgcg caccgccatc ttccctctgg
     1621 ccgccgagtg atcgttttcg aacatattga tgaccgcctg ccagaaaagg gccaagtcct
     1681 cttccgaaaa gccggtggtc ttgcgcgcca ggttggcgga gatgaacccc tcggcccggt
     1741 agaggccgta agggatgatg tacttgcggc ccatctcggt gccctttctc tccgcgtccg
     1801 tctccgtggt gatcgccacg cgggtgatgg tgatctcctg ggggatcagc gggtcgatcg
     1861 agcgggcaaa gcccagctgt accggtcccc gaacctggcc gcagttgagg gcggccttga
     1921 caaaggtggt catcaccgcg ccgaacgtgc ggatgtcata aaaattttga cacatgaagt
     1981 cgcggatctt gcagtccagc tccgggtctt ttttcttgtc ctctttcacc tttttgtcgt
     2041 cggtgccgag ataggcatag gcctccgcat cgctgcggtt gaggggcacg ccgtccttga
     2101 tatagatgcg gtatccggcc tcgccctcct tgacggtctc cacatagttg cgtatctttc
     2161 gtttgaggca cacatcggtg acgatgccgt agccggtctc cgggtcgagg cggggcatat
     2221 tgcccgcgtc cgggtcgccg ttggggttgc cgttttccac gtcgaagagc accacaaatt
     2281 catacctgtt tttgatcgcc tcagccattt tccttgtcct ccctgtcttc ttttttcgtg
     2341 tagcgctgct gggtctgata gtagtaaccg agatagaatg tcccttggtc tttgatct
//
LOCUS       DBMDYA010000467         2396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9425_length_2396_cov_1.780828, whole genome shotgun sequence.
ACCESSION   DBMDYA010000467 DBMDYA010000000
VERSION     DBMDYA010000467.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_2396_cov_1.780828"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="ACZPFK_10675"
     CDS             <1..393
                     /locus_tag="ACZPFK_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1406820.1"
                     /translation="YTYLLCAEKKASVVWIPIEVLDEMLTRDPQLAGALLRYVSQRGL
                     ENQLYLNCMNYQTIRQRIAYWAVGIQRIEPGRAVRLPPSQTIFANLLHVSRPSLNQEL
                     KQMEREGYFRLESKKMCDVDTLRLSRLL"
     gene            471..1151
                     /locus_tag="ACZPFK_10680"
     CDS             471..1151
                     /locus_tag="ACZPFK_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406821.1"
                     /translation="MAHIMSFFKFLFSKITKKGLSIAAGCAAGACIFGMSLYIAYLSG
                     QNSVLQAAQNAQGDPSQPASSSSSSAAAPASSSASSGSFTPSSVPAPTEAPPTPTSVP
                     LPDNSQRISAIRAEIASLQTQIDAANATYNATVPPLQAAADAESAQEQALWDAQIAAR
                     READAHPDLEGKQAAARNTAANYSACIGRKDAALAGIAQAQADLDAALGSANIRLAEL
                     QAELAALQ"
     gene            complement(1421..1767)
                     /gene="ssrA"
                     /locus_tag="ACZPFK_10685"
     tmRNA           complement(1421..1767)
                     /gene="ssrA"
                     /locus_tag="ACZPFK_10685"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1830..2294)
                     /gene="smpB"
                     /locus_tag="ACZPFK_10690"
     CDS             complement(1830..2294)
                     /gene="smpB"
                     /locus_tag="ACZPFK_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1406822.1"
                     /translation="MAENFKTIADNRKARHDYFVLEAMEAGIELVGTEVKSIRTGSVN
                     LKDSWVDVVNGELMVLGMHISPYEKGNIFNRDPVRPRRLLAHKKEIRRLAAEIKQNGL
                     TLVPLKLYFKGSRVKVEVGLCKGKKLYDKRATAAQKDAKRDIDRAMKSHSVG"
BASE COUNT          506 a          722 c          625 g          543 t
ORIGIN      
        1 tatacctatc tgctctgtgc ggagaaaaaa gcctcggttg tctggatccc catcgaggtg
       61 ctggacgaga tgctcacccg cgacccgcag ctggcggggg cgctgctgcg ctatgtgtcc
      121 cagcgggggc tggaaaatca gctctacctg aactgcatga attaccagac catccgccag
      181 cgcatcgcct attgggcggt gggcatccag cgcatcgagc cggggcgcgc ggtgcgcctg
      241 cccccctcgc agacgatctt tgccaacctg ctgcatgtga gccgcccctc gctgaaccaa
      301 gagctgaaac agatggagcg cgagggttat tttcggctgg aaagcaaaaa aatgtgcgat
      361 gtggacactc tgcggctgag caggctgctg taagggcgtc cgtttatcca acccaaagag
      421 gcaagcggct ccctgcagct tgccataaac gtcaaaaaag tgtggttatt atggcacaca
      481 tcatgagttt tttcaaattc cttttttcca aaattacaaa aaaagggtta tccatcgcgg
      541 cgggatgcgc tgcgggagcc tgcatcttcg gcatgtctct ttatattgcc tatctgtccg
      601 gccaaaacag cgtattgcaa gccgcgcaaa acgcccaagg cgatccctcc caaccagcct
      661 cttcttcaag ctcatctgcc gccgcaccag cttcctcatc ggcttcttcc ggcagtttca
      721 cgccctcttc tgtcccggct cctacggagg cacctcctac gccaacctcg gttcccctgc
      781 cggataactc gcagcgaatc agcgctatcc gcgccgagat tgcttctctt cagactcaga
      841 tagacgccgc aaatgccact tacaatgcca cggtgccgcc cctgcaagct gccgcagatg
      901 cggagtcagc gcaagagcag gccctttggg atgcccaaat cgctgcgcgg cgggaagcgg
      961 atgcccaccc cgatctagag ggcaaacagg cagcagccag aaacacggca gccaattact
     1021 ctgcgtgtat cggccgaaaa gacgccgctc ttgccggcat tgcccaagca caggccgatt
     1081 tggatgcggc tctcgggtcg gccaacatac ggcttgcgga attacaggcc gaactcgccg
     1141 cacttcaata attactataa acggctaacc gtcaaatttt tcctcgaggg gcaagctcag
     1201 cctcctgtcg accactcaaa aaagcatgga tactttcggc gtcaacggtt attctctcac
     1261 cgacaaagcc aacaggcggc ggaggattta ggaaaaattt ttgacaaggt tatttctctt
     1321 gcttaaaagt gggttaaaaa atgggttatc ccaacaaatt caacaagata aaaagaccga
     1381 acaaacagct ttataaggcc atttgttcgg tctttctctc tggtggagat gacgggagtc
     1441 gaacccgtgt ccaaaaagag ttctgtcaga gtatctccgg gtgcagctta tcattgggat
     1501 tcccccgcct ggccgccggt aagcaggccg ccagggtcgg tagcttcatg agttcatgac
     1561 ggtccgcaaa gcttagggcc gttcacgtgc accgcttatt ttatgcccaa gcaccacccc
     1621 gcggtaaggt gggaaggaca gcagcctaat ttaggctgcg actgctaatt tattgttgtt
     1681 agctattttt ttgcggcttt ttgagtgggt ccgcaccact acccgctgct ccggcttcgc
     1741 tctccctgtc gaaaccttta catccccctg tattgcccgg cccgcgccgg gcggtttggt
     1801 gtgaaaaaat actgctgcgc acagcgcgtt cagccgacgc tgtgcgattt catggcgcgg
     1861 tcgatatccc gctttgcgtc cttttgggct gcggtggcgc gcttgtcgta cagctttttg
     1921 cccttgcaaa gccccacctc cactttgacc cgcgagccct tgaagtagag tttgagcggt
     1981 actaaagtga ggccgttctg cttgatctcc gccgcgaggc ggcgaatctc ctttttgtgg
     2041 gccagcagcc tgcgcggccg caccgggtcc cggttgaaga tattcccttt ctcgtaaggg
     2101 ctgatgtgca tgcccagcac catgagctcg ccgttcacca catccaccca ggaatctttc
     2161 agattcaccg agccggtgcg gatgctcttg acctcggtgc cgaccagctc gatgccggcc
     2221 tccattgcct cgaggacaaa atagtcgtgc cgcgccttgc ggttatccgc gatcgtcttg
     2281 aaattttcgg ccattccgtc cccctccttt cggcctacat caggctaata ctatacccgt
     2341 tttgcaggcc gctgtcaagg ggaaagcgca aggccctaca gctccccgcg ggaaat
//
LOCUS       DBMDYA010000468         2396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9427_length_2396_cov_1.290196, whole genome shotgun sequence.
ACCESSION   DBMDYA010000468 DBMDYA010000000
VERSION     DBMDYA010000468.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9427_length_2396_cov_1.290196"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="ACZPFK_10695"
     CDS             complement(<1..1137)
                     /locus_tag="ACZPFK_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1406823.1"
                     /translation="MAKKQPKKKAPLQRPQLGDAVEILDAGSVIESRITETLESNYMP
                     YAMSVIVSRALPEIDGFKPAHRKLLYTMYQMGLLKGNRTKSANIVGSTMHLNPHGDAA
                     IYDTLVRLSRGNESLLLPYISSKGNFGKAYSRDMAYAASRYTEAKLEDVCRELFADID
                     KDTVDFVDNYDATTTEPTLLPVTFPAILANNTLGIAVGMASNICSFNLAELCETTIAL
                     LRDKKHDILSTLKAPDFVGGGQLLYDEGELRSIYETGRGSVRVRARYQYDRENNMIEI
                     TQIPPTTTVEAIMDKIAELVKAGRVREISDMRDETDLGGLKLTLDLKRGQDPDKLMGR
                     LFKMTPLEDSFSCNFNILIAGQPRVMGVREILAEWSAFRTECVRR"
     gene            complement(1203..>2396)
                     /locus_tag="ACZPFK_10700"
     CDS             complement(1203..>2396)
                     /locus_tag="ACZPFK_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1406824.1"
                     /translation="KVKMSAAFCFSNKRQVLEYYHNSSWLEHGGSPERAVKTAFVSQI
                     DAYLKNKCLYKKGESKITFADVQDCLIYVSSSFSTVTSYENQTKKAITNRGIAQVMTE
                     FLKHTLEVYFIENPDEAQKLCSQVLVNKQSREHAEKTRLSIKKTLTSQIDIANRVAKF
                     VDCRTKDLTRRELYIVEGDSAMGAVKTSRDSEFQAIMPIRGKILNCLKSDYSRIFKSD
                     IITDLVKVLGCGVELDGKVKNDFAAFSMDNLRWSKVIICTDADVDGYQIRTLVLTMLY
                     RLVPTLINEGYVYIAESPLYEIESKGKTYFAYTEPEKTDILARIGQDKYTIQRSKGLG
                     ENEPEMMWLTTMNPETRRLIKVLPEEAQRTAEVFDLLLGDNLAGRKEHIATHGYEYLD
                     DLDVS"
BASE COUNT          389 a          710 c          749 g          548 t
ORIGIN      
        1 ccggcgcacg cactcggtgc ggaaggcgct ccactcggcc agaatctccc gcacccccat
       61 cacccggggc tgtcccgcga tgaggatgtt gaagttgcag gagaagctgt cctccaacgg
      121 cgtcatcttg aacagccgtc ccatcagctt gtcggggtcc tgcccgcgct tgaggtccag
      181 cgtcaatttc aggccgccca ggtcggtctc gtcgcgcatg tcgctgatct cccgcacccg
      241 gcccgccttg accagctcgg cgatcttatc catgatcgcc tccaccgtgg tggtgggggg
      301 gatctgggtg atctcgatca tgttgttctc tctgtcgtac tgataccgcg cccgcacccg
      361 cacgctgcct cgcccggtct catagatgga gcgcagctcc ccctcatcgt agaggagctg
      421 gccgccgccg acgaaatccg gcgctttcag ggtggagagg atatcgtgct ttttgtcccg
      481 cagcagggcg atggtggtct cgcacagctc ggccaggttg aacgagcaga tgttggaggc
      541 catgcccacc gcgatgccca gcgtgttgtt ggccaggatg gcgggaaagg tcaccggcag
      601 cagggtgggc tcggtggtgg tggcgtcgta gttgtcgaca aaatccaccg tgtccttgtc
      661 gatgtcggcg aacagctcgc ggcagacatc ctccagcttg gcctcggtgt agcgggaggc
      721 cgcgtaagcc atgtcgcggg aataggcctt gccgaagttg cccttgctgg agatataggg
      781 cagcagcagg ctctcgttgc cgcgggacag gcgcaccagc gtgtcgtaga tggccgcgtc
      841 gccgtggggg ttgaggtgca tggtgctgcc cacgatgttg gccgacttgg tgcggttgcc
      901 cttgagcagg cccatctggt acatggtata cagcagcttg cggtgcgcgg gcttgaagcc
      961 gtcgatctcg ggcagcgcgc gggagacgat gacgctcatc gcgtagggca tgtaattgct
     1021 ctccagcgtc tcggtgatgc ggctctcgat gacgctgccc gcgtccagga tctccaccgc
     1081 gtcgccgagc tgcggccgct ggagcggcgc ttttttcttg ggctgttttt ttgccattct
     1141 tttccctctt acagtttatt cacaaggctc gcacagatgc gcggccgaaa gccccggccc
     1201 cgtcaggaca catccagatc gtccagatac tcgtagccgt gggtggcgat atgctctttg
     1261 cggcccgcaa ggttgtcgcc cagcagcagg tcaaagacct ctgccgtgcg ctgggcctcc
     1321 tcgggcagca ccttgatgag gcggcgggtc tcggggttca tggtggtcag ccacatcatc
     1381 tccggctcgt tctcgcccag ccccttggag cgctggatgg tgtacttgtc ctgcccgatg
     1441 cgggccagga tgtccgtttt ttccggttcg gtataggcaa aataggtctt gcccttgctc
     1501 tcgatctcat acaggggcga ctctgcgatg tagacgtagc cctcgttgat gagggtgggc
     1561 accagccggt agagcatggt gagcaccagc gtgcggatct gatagccgtc cacgtcggcg
     1621 tcggtgcaga tgatgacctt gctccagcgc aggttgtcca tgctgaaagc ggcaaaatca
     1681 tttttcacct tgccgtccag ctccacgccg caccccagca ccttgacgag gtcggtgatg
     1741 atatcgcttt tgaagatgcg gctgtaatcc gatttgaggc agttcaaaat tttgccgcgg
     1801 atgggcatga tggcctgaaa ctcgctgtcc cggctggtct tgaccgcccc catagcggaa
     1861 tcgccctcca cgatatagag ctcgcgccgg gtgaggtcct tggtgcggca gtcgacaaac
     1921 ttggccaccc ggttggcgat gtcgatctgg gaggtcaggg tctttttgat ggacaggcgg
     1981 gtcttttcgg cgtgctcccg gctctgctta ttgaccagca cctggctgca gagcttttgc
     2041 gcctcgtctg gattctcgat gaaatagacc tccagcgtat gcttcaaaaa ctcggtcatc
     2101 acctgggcga tgccccggtt ggtgatggcc tttttggtct ggttctcata gctggtcacg
     2161 gtggaaaagc tgctggacac atagatgaga cagtcctgca cgtcggcaaa ggtgattttg
     2221 ctctcgccct ttttgtacag gcacttgttc ttgaggtagg cgtcgatctg gctgacaaag
     2281 gccgttttca ccgcccgctc ggggctgccg ccgtgctcca gccagctgga gttgtggtaa
     2341 tactccagca cctgccgctt gtttgaaaag cagaacgcgg cgctcatctt cacctt
//
LOCUS       DBMDYA010000469         2394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9435_length_2394_cov_1.370257, whole genome shotgun sequence.
ACCESSION   DBMDYA010000469 DBMDYA010000000
VERSION     DBMDYA010000469.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_2394_cov_1.370257"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="ku"
                     /locus_tag="ACZPFK_10705"
     CDS             complement(<1..573)
                     /gene="ku"
                     /locus_tag="ACZPFK_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR1406825.1"
                     /translation="MAVAYKGNIVFGLVQIPVTLHTATRDLDVRFNQLHRGDHRRIRY
                     KKVCGHCQKEVTGKDIVRGFEYADDKFVIITDEDLARIKTPEDRTVNVLQFVDRGAVS
                     PVYFDRTFHAAPATGGEKAYRVLADAMRETHTAALCRTVMENRDTLVLLVPDEEGILA
                     ETLFYDEEVRRLPKISVTEAPATAELKMAKT"
     gene            complement(1046..1849)
                     /locus_tag="ACZPFK_10710"
     CDS             complement(1046..1849)
                     /locus_tag="ACZPFK_10710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1406826.1"
                     /translation="MTQPLVSVIVPVYNAAPDLARCIESVRRQSYERIEVLLVNDGSS
                     DASLPICQMYARVDARVRVIDRLNSGVSAARNAALAQAAGEYLQFVDSDDYLDPDATR
                     LLVERALQTGADMVISHYWRVIGEEKTEHGFLCGDAVMDQVQFAKNLMEEPASFYYGV
                     MWNKLYRREIIRRHGIQCNVELGWSEDFLFNLEYIRYARQFAALPTPIYYYVKNEKSI
                     TATQIHWRSALETKRSLFAYYRDLYTSLGLYEKYKPQIFKYLIASAEHP"
     gene            complement(2167..>2394)
                     /locus_tag="ACZPFK_10715"
     CDS             complement(2167..>2394)
                     /locus_tag="ACZPFK_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406827.1"
                     /translation="ASYEALMLACADKLSNLRSIAADYEAEGEAVWNRFKRGRAQQRW
                     YYAEMLRAFAPLADTEMYREFAGLFEEVFGC"
BASE COUNT          402 a          803 c          705 g          484 t
ORIGIN      
        1 ggtctttgcc atcttcagct ccgccgtcgc cggggcctcg gtgacgctga tcttgggcag
       61 ccgccgcacc tcctcgtcgt aaaaaagcgt ctcggccaga atgccctcct cgtccggcac
      121 caaaaggaca agggtgtccc ggttttccat caccgtgcgg cagagcgcgg cggtgtgggt
      181 ctcgcgcatg gcatcggcca gcacgcgata ggccttttcg ccgcctgtcg cgggcgcggc
      241 gtgaaaggtg cggtcaaaat aaaccggcga gaccgcgccc ctgtccacaa attgcagcac
      301 gttcaccgtg cggtcctccg gcgttttgat gcgggccagg tcctcgtcgg tgatgatgac
      361 gaacttgtcg tccgcatatt caaagccgcg cacgatatcc ttgccggtga cctccttttg
      421 acagtggccg caaacctttt tatagcggat gcggcggtga tccccccggt gcagctggtt
      481 aaagcgcaca tccaggtcgc gggtggcggt gtgcagcgtc accgggatct gcaccaaacc
      541 aaagacgata ttgcctttat aggcgacagc cattttaaaa cgcctccttt tccggggggc
      601 gcacagccgc cggcaaaact gcccgcaggc catgaaaaaa gcctcccgcc ggtagcgttc
      661 ccggcgggag gcttttttat gtgagaccag agactgcgcg cacggcgcct gcctttttta
      721 ttcaggcggc tgccgcgccc gcatttgcgc aaggggccct atgccgccct gagcggaatt
      781 tttctccatg cgagaacgtc gggccgttcc cgcatggacg gccgctgcgc ttgaggttac
      841 tgcgttgctt gtggccgccc ggagcggagc ttccctcttt ccgagaacgg caacggccgt
      901 tctcgcatgg acggccgctg cgcttgaggt tactgcgttg cttatagccg tcctgagcgg
      961 agcctccctc tttccgagaa cggcaacagc cgttctcgca tggacggccg ctgcgcttga
     1021 ggttactgcg ttgcttatag ccgtcctaag gatgctccgc gctggcaatc agatatttaa
     1081 aaatctgcgg cttatacttt tcgtacaggc caaggctggt gtagaggtcg cggtaatagg
     1141 cgaacaggct ccgcttggtc tccagcgcgc tgcgccagtg gatctgggtg gcggtgatgc
     1201 tcttttcgtt ctttacataa tagtagatgg gggtgggcag cgccgcaaac tgccgggcat
     1261 agcggatata ttccagattg aagagaaagt cctcactcca gcccagctcc acattgcact
     1321 ggattccgtg ccggcggatg atttcccgcc ggtagagctt gttccacatc acaccgtaat
     1381 aaaagctggc cggttcctcc atcaggtttt tggcgaactg tacctgatcc atcaccgcgt
     1441 cgccgcagag gaagccgtgc tcggtctttt cctccccgat cacccgccag taatgggaga
     1501 tgaccatgtc cgccccggtc tgcagcgccc gttccaccag cagccgtgtg gcgtccgggt
     1561 ccaggtagtc gtcgctgtcc acgaattgca gatactcccc cgccgcctgc gcgagggcgg
     1621 cattgcgggc ggccgacacc ccgctgttaa gccggtcgat cacccgcact ctggcatcca
     1681 cgcgggcgta catctggcag atgggcaggc tggcgtcgct gctgccgtcg ttgaccagca
     1741 gcacctcaat gcgctcatag ctctgccgcc gcacgctctc gatgcagcgc gccaggtccg
     1801 gcgcggcgtt atacaccggc acgatgacgc tcaccagcgg ctgtgtcatc cctttctccc
     1861 cctctttcgc cgggccgggc ccggcgtttt ttctcagact ttcaagctca aaacagcaaa
     1921 tccaaaccgg gctcggcgac cccctggccc ggcaccggcc gtctttatcc ccagcaccag
     1981 acgaatcagc ggggccgccg tcccgcccgc atcccccgcg gctgcggcaa gctctccgcc
     2041 gtcgggtatg ccgcagcgcg gtgcgccaca gggcccgcgc cgggggcctg gcggtctttt
     2101 tccctggcag ggcgttcccg caaaacggcg gctgcggtct ccgccccttt cgctcacggc
     2161 ccggcctcag cagccgaaga cctcttcaaa caacccggca aactcgcggt acatctccgt
     2221 gtccgccagg ggggcaaagg cgcgcagcat ttcggcataa taccagcgct gctgtgcccg
     2281 cccgcgcttg aaacggttcc acaccgcctc tccctcggcc tcatagtccg ccgcgatgct
     2341 gcgcaggttg gacagcttgt cggcgcaggc caacatcagc gcctcatagg acgc
//
LOCUS       DBMDYA010000470         2387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9456_length_2387_cov_1.669729, whole genome shotgun sequence.
ACCESSION   DBMDYA010000470 DBMDYA010000000
VERSION     DBMDYA010000470.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9456_length_2387_cov_1.669729"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(373..1665)
                     /locus_tag="ACZPFK_10720"
     CDS             complement(373..1665)
                     /locus_tag="ACZPFK_10720"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR1406828.1"
                     /translation="MDHKLICEILNSEMELATGCTEPAAIALCAANARAHLKGEVAEL
                     TVLASVNIIKNAMAAGIPGTSYTGIDYAAALGAVGGKVERQLQVVDEASEEERRQAVA
                     LVQGGHVRMGKKDTPEKLYVEVEVKSADGHQARAIIASAHTNVVRIEEDGVAVVDHEL
                     GTLVNGVPVDVIDETLSIQTIFDFVNELDREKDDLHMIEQAIAVNTRIAEEGMKGDYG
                     LNIGRNIAKAKKAGYMSDDLVTNAMEVTASGADARMAGANVPVVTNSGSGNQGITATM
                     PVVSAAKTLGIDEDKMFRAVTLSHLIGIHIHCRFGLLSGLCGATVAGTGSACGLVYLL
                     GGSAQQIGYAVNNMLGDVTGMLCDGAKADCALKISTCVNTAFQCAFMALDNVCVQPSD
                     GIVDKDPEQTIKNFVRLGNEGSPVMDDIVLDVMLTKQK"
     gene            complement(2025..>2387)
                     /locus_tag="ACZPFK_10725"
     CDS             complement(2025..>2387)
                     /locus_tag="ACZPFK_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406829.1"
                     /translation="ALMMLVFDGLKNAQPADIVAMIVPMIVVIVVGVLGMGIVSFLIS
                     KVLKVGPAMAFATALTALYGFPPNLILTTEACKALAESPEENDYLMANMLPQMIVGGF
                     TTVTISSVLIAGVFANWL"
BASE COUNT          416 a          753 c          702 g          516 t
ORIGIN      
        1 cgcttcccaa tctccgggcc ggagagccgc cccaacatca ggtttgaaag ccgcttcccg
       61 cctcctgttt ttgtgtgccc gggaattttt ccctctttcc ccccggccgt cgggatgcgg
      121 tgtggcgggc aggtttttta gcgtggctcc gcccctccgg acgcgggagc cggcccgctt
      181 cctgtcgggc cgctgggccg cgtttttttc cggcttctcc gcgcggcggc gagagcattc
      241 ccacggcagc gaagaagaaa aaagagaaaa gagccgcccg gcaacagccg agcagctctt
      301 tcagcgctct gtggggacgg agcaaactgc cgcctcccgt actttttgga ggctcggaat
      361 gcgccgaggc ccttatttct gctttgtcag catcacgtcc aacacgatgt cgtccatgac
      421 gggggagccc tcgttgccca ggcggacgaa gtttttgatc gtctgctccg ggtccttgtc
      481 cacgatgccg tcgctgggct gcacgcagac gttgtccagc gccatgaacg cgcactggaa
      541 cgccgtgttc acgcaggtac tgatcttcag cgcgcagtcc gccttggcgc cgtcgcagag
      601 catgccggtg acgtcgccca gcatgttgtt gaccgcatag ccgatctgct gcgcgctgcc
      661 gcccagcagg tagaccaggc cgcaggccga gccggtgccg gccacggtgg cgccgcacag
      721 gccgctcagc aggccgaagc ggcagtggat gtggatgccg atcagatggg agagggtgac
      781 ggcgcggaac atcttgtcct cgtcgatgcc cagcgtcttg gcggcgctga ccaccggcat
      841 ggtggcggtg atgccctggt tgcccgagcc ggagttggtg accacgggca cgttggcgcc
      901 cgccatgcgc gcgtcggcgc cgctggcggt gacctccatg gcgttggtga ccaggtcgtc
      961 gctcatgtag cccgcctttt tcgccttggc gatgttgcgg ccgatgttca ggccgtaatc
     1021 gcctttcatg ccctcctcgg cgatgcgggt gttgaccgcg atggcctgct cgatcatgtg
     1081 caggtcgtcc ttttcgcggt ccagctcatt gacgaaatca aagatcgtct ggatggagag
     1141 ggtctcgtcg atcacgtcca ccggcacgcc gttgaccagg gtgcccagct cgtggtccac
     1201 cacggccacg ccgtcctctt caatgcgcac cacattggtg tgggcggagg cgatgatggc
     1261 cctggcctgg tgcccgtcgg cgctcttgac ctccacctcc acatacagct tttcaggggt
     1321 gtcctttttg cccatgcgca catggccgcc ctgcaccagc gcgaccgcct gacggcgctc
     1381 ctcctcgctg gcctcgtcca ccacctgcag ctggcgctcc accttgccgc ccacggcgcc
     1441 cagcgccgcg gcgtagtcga tgcccgtata gcttgtcccg gggatgcccg ccgccattgc
     1501 gtttttgatg atgttgacgc ttgccagcac cgtcagctcc gccacctcgc ctttcaagtg
     1561 agccctggcg tttgcggcac aaagcgcaat cgcggcaggc tcggtacatc cggtggcgag
     1621 ttccatctcg ctgttgagaa tctcacagat cagcttgtgg tccataagta tctcctcctt
     1681 aaattttagt ttatcctcgc cgcctgggac ggcgatcttt accgcacaaa agatcaggaa
     1741 ctttttattc acaatctttt cataattata ccgtcaaggg catacaaatt caattctttt
     1801 tcttttttat ggtggaacta ccaccatttt cacaaaattg cttctacaag tttagtcatt
     1861 gcgtatatgg aaaagccaca ggcctttttc tgcatttcag agcataaaaa gacccccgcg
     1921 gaacagcgcc tgcgagggcc ttttttattt accgaactca ttttacagca aagggaaagg
     1981 acctttggcc aatcccgcgc gccgcgcggc aaggcccccg ctgcttagag ccagttcgcg
     2041 aaaacgcctg cgatcagcac tgaggagatg gtgacggtgg tgaagccgcc gacgatcatc
     2101 tggggcagca tattggccat cagatagtcg ttctcctccg ggctctcagc cagggccttg
     2161 caggcctcgg tggtgaggat caggttcggc gggaagccgt acagggcggt cagtgcggtg
     2221 gcaaacgcca tggccgggcc aaccttgagc accttgctga tgaggaaaga tacaatgccc
     2281 atgcccagca cgccgaccac aatgacgacg atcatgggta cgatcatggc cacgatgtcc
     2341 gcaggctgcg catttttgag gccgtcaaag acgagcatca tcagcgc
//
LOCUS       DBMDYA010000471         2386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9460_length_2386_cov_1.595186, whole genome shotgun sequence.
ACCESSION   DBMDYA010000471 DBMDYA010000000
VERSION     DBMDYA010000471.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9460_length_2386_cov_1.595186"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="ACZPFK_10730"
     CDS             <1..1454
                     /locus_tag="ACZPFK_10730"
                     /inference="COORDINATES: protein motif:HMM:NF046734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6973 domain-containing protein"
                     /protein_id="HIR1406830.1"
                     /translation="GEPGFEQKVALVQQALQRDREAHRRQEGNHYTAGNTAGRGSGSG
                     GFGGGNLSGESFPGAGTSGVPGGPAVGRSRPAFPTAGQGGAGQGAFHFPRTLPQKGQF
                     KSGRLNMPERGGVFGQGGAPRLPGYGGEWNRPQRVPVTYGPGEAPQRQHRPQWGQDGQ
                     GGGRLEVTKRPSDNASLDRWRERKWNDEREIERQKPSVTSIYAKDNNGFVKEPAGNSL
                     KKLSGYDTLKKNGEEEKRRRLPDKKLFLKVKDEEKKEPDYTDSEWWLQHVEDLEEQFG
                     IDDLPLVGDLIAGWWGEEADKAKLKPAEKQVAFSHPLATAQLSFFKVHEKAGQAKSIV
                     FAGAEDLNVDGARPNAFLHAYWNALMAYYIGASNAEEFATAHETYTKEQYNDEKILET
                     IADNFPIGEQAAMDMHNNEVGRALAAKMPVGPTAPLTNMLVLETLLKNYYDGSMEKLN
                     EAKKEYPGFSNAELLLLHYTADELTNGNLVWLQ"
     gene            1688..2173
                     /locus_tag="ACZPFK_10735"
     CDS             1688..2173
                     /locus_tag="ACZPFK_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406831.1"
                     /translation="MKKVLLAVLCVLLGFCLLVAAGLVCLYFMFPVDEEARFRVEQER
                     KLTEYAQTHEEFTLGEILEFEWDTAYNYPHGYGTGTPLKEKYGLEFEIPEANDEVRGQ
                     LIFFKDGEFVINLIYERFSFRLDNRVEVFTPETVFILKKEGETKRLIPKNPDDVVWAD
                     P"
BASE COUNT          624 a          534 c          756 g          472 t
ORIGIN      
        1 gcggcgagcc gggctttgag caaaaagtgg cgctggtgca gcaggcgctg cagcgggacc
       61 gggaggcgca ccgccgacag gaggggaacc actacacggc ggggaacacg gcgggccgcg
      121 gcagcggcag cggcggcttt ggcggcggca acctgagcgg ggagagcttt cccggcgcag
      181 ggacgagcgg cgtccccggc gggcccgcgg tcggccgcag ccgcccggcc tttccgacgg
      241 cgggccaggg cggcgcgggg cagggggctt ttcactttcc ccgcaccctc ccgcagaaag
      301 gccagtttaa aagcggccgc ctgaatatgc ccgaaagggg cggcgttttt ggccaaggcg
      361 gcgcgccgcg cctgcccggg tatggcggtg aatggaatag gccgcagagg gtgcccgtga
      421 cctacgggcc gggggaggcc ccccagaggc agcaccggcc gcagtgggga caggacggac
      481 aagggggagg caggctggag gtaacaaaga gaccaagcga caacgcttct ttggacagat
      541 ggagggagag aaagtggaat gatgagagag agattgagcg tcaaaagcct agtgtcacgt
      601 ccatatatgc aaaagacaat aacggatttg taaaagagcc ggcgggaaat tcgttgaaaa
      661 aactgtctgg gtatgatacc cttaaaaaaa atggggaaga agaaaagcgt aggcgtttac
      721 cggacaaaaa actttttctt aaggtcaagg atgaagagaa gaaagaaccg gattatacgg
      781 acagcgagtg gtggcttcag catgtggagg atttggaaga acagtttggc attgatgatc
      841 tccccctggt tggagacctt attgccggct ggtggggaga agaagcggac aaagcaaagt
      901 taaaaccggc tgaaaaacaa gtggcattca gtcatccgct tgccacagcc caactgagtt
      961 tttttaaagt acatgaaaaa gcaggtcaag caaaaagtat tgtctttgcc ggtgcggaag
     1021 accttaatgt ggacggcgca cggcccaatg cttttttgca tgcatactgg aacgctttga
     1081 tggcgtatta tataggcgct tccaatgcag aggaatttgc tacggcccac gagacctata
     1141 caaaagaaca gtataacgac gaaaagatat tagaaacaat tgcagataat ttccctattg
     1201 gagaacaggc ggcgatggat atgcataata atgaggttgg acgggcgtta gctgccaaaa
     1261 tgcctgtggg ccccacggcc ccgctgacca atatgctggt tttagaaacc ttgttgaaaa
     1321 attattacga cggttcaatg gaaaagctga acgaggcaaa aaaagagtat cccggtttca
     1381 gcaatgcgga acttttgctg ctgcattata cggcggacga gttgacaaac ggcaatttgg
     1441 tctggctgca atgataaaga atagccgcgg gctatttttt tcggccaccc gtttcggttg
     1501 tcgcgacagc ggcgagaaac ggggccatta agggcaattg ttttttacgc gcatccccca
     1561 ctccgtgggc aggcgctcaa gattttaagg ccgtggagcg ggacgcccca catggctgtt
     1621 tttatagttt tgcgatgcca aactataaaa atgcggaaca agaaatggag cggacatgaa
     1681 aaggaagttg aaaaaagtgc tgttggctgt gctctgtgtg ctgttgggct tctgcctgct
     1741 ggtcgctgcg ggcctggtct gcctgtattt catgttcccg gtggacgaag aagctcggtt
     1801 tagagtggaa caagagcgaa aactgactga gtacgcacag acgcacgagg aatttacact
     1861 gggggaaata ctagagttcg agtgggacac ggcctataat tacccgcatg gatatggtac
     1921 tggaacaccg ctgaaagaaa aatacggtct ggaatttgag atccctgagg caaacgatga
     1981 agtgcgcgga cagttgattt tttttaaaga tggagagttc gtcatcaatc tgatctatga
     2041 gagattcagt ttccgattgg acaatagggt tgaggtcttt actccggaga cggtttttat
     2101 attaaaaaaa gaaggagaaa cgaaaaggct tatcccgaaa aaccccgatg atgtggtgtg
     2161 ggcagaccca taggcacggc aagccgcggg gcttgccggt cagtctgcgc caaagaccgg
     2221 tttgggaatc tgcaaaattg agataaggac cggcggagag ggtgctctcc gccggtcctt
     2281 tttgcctgcg acggtttttc accggcccgg cggcgagagg gcgggcgctg gcagccgccg
     2341 gagaggggcg cgccccacac cttgatacag ccgtgagaga aaaggg
//
LOCUS       DBMDYA010000472         2375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9501_length_2375_cov_1.543536, whole genome shotgun sequence.
ACCESSION   DBMDYA010000472 DBMDYA010000000
VERSION     DBMDYA010000472.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9501_length_2375_cov_1.543536"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="ACZPFK_10740"
     CDS             complement(<1..896)
                     /locus_tag="ACZPFK_10740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042577 - lipid phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046839 - phospholipid dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1406832.1"
                     /translation="MKLKKDQKLLLAGLGLLAALLLAAAAFYDLAIDRALYSPQNPFG
                     IVLEAFCYWPLYLPFVLLGAVWTFLYRDNPSRHVLGEVLVIVVFYVLFTQSLPNLARR
                     GLLPLSGTALTLAAVVLTFVASVAVISLASRWDRPSLARLDFLAKLGIALCVADNVVI
                     NLLKVLWTRMRFDDMAAAGDFSAFTPWYRWGAVAGNTSFPSGHTAAACGVLVLLALPA
                     LFDWWKGKELALTCGCYAYITLTALSRLIMGRHYLSDTVAAAVIMTLLYLALTHTRRC
                     RRGLEKALALPPAPPAADAPREG"
     gene            complement(904..1572)
                     /locus_tag="ACZPFK_10745"
     CDS             complement(904..1572)
                     /locus_tag="ACZPFK_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406833.1"
                     /translation="METARSSDNFFIRILLFILLSGFMLPRLPGKLDGFIIIKCPGPA
                     KSTVFVRFLFLCAFLQNNFFQFLISLQIFSSLKVFTLIFPTFYICFKQIYEKKRSKLP
                     NIAFSVFLLSKNLKRKQKKAIAFQRRTMLAFIMLFFRLSRSGAILPLKLVQKSTFAMF
                     FLFLLSSRFVWVRDRGRRASAFLDTPAKIWYTVSCRRRVFSAADGRRERGAPASRAAG
                     PERV"
     gene            1538..>2375
                     /locus_tag="ACZPFK_10750"
     CDS             1538..>2375
                     /locus_tag="ACZPFK_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1406834.1"
                     /translation="MKKLSLLLAVSMLMTLLAGCGGGASSGSGGPSSGSSPGAGTSQA
                     AAGAYVIANTPKCVGISWWDRMLVGNDRFVASTGNEVYQVGPAGQSDTAVQVSTIEDV
                     IATGVDAITVIPSDPDAVETTLAKAREQGIVVISHEAEGMQNVDYDLEAFVNREYGAH
                     MMDVLAKEMGEEGGYCIMMGMLTMSSHQQWTEGAIERQKEAYPNMYMVCDPVESQAGG
                     GSQEGSYKVAKEVIASYPDVAGFIGCDMVNPPGIAMAVEEAGKAGEIAVTGTCLVSVA
                     GEY"
BASE COUNT          543 a          672 c          771 g          389 t
ORIGIN      
        1 ccctcccggg gcgcgtccgc cgccgggggc gcgggcggca gcgccagggc cttttccagc
       61 ccgcggcggc agcgccgcgt gtgggtgagg gccagataga gcagggtcat gatcaccgcc
      121 gccgccaccg tgtcggacag gtaatgccgc cccatgatga gccgtgagag ggcggtgagg
      181 gtgatgtagg cgtagcagcc gcaggtgagg gcaagctcct tgcctttcca ccaatcgaac
      241 agggccggca gggccagcag caccagcacc ccgcaggcgg ccgccgtgtg cccggagggg
      301 aagctggtgt tgcccgccac ggcgccccag cggtaccagg gggtaaaggc ggaaaaatcc
      361 cccgccgccg ccatgtcgtc gaaccgcatg cgggtccaca gcactttcag caggttgatg
      421 accacattgt cggccacaca gagcgcgatg cccagcttgg ccagaaagtc gagccgggcc
      481 agcgatggcc ggtcccagcg gctggccagg ctgatgaccg ccaccgaggc cacaaaggtc
      541 agcaccaccg ccgccagcgt gagcgccgtg ccggacaggg gcagcaggcc gcgccgggcc
      601 aggttgggca gcgactgggt gaacagcacg taaaagacca cgatcaccag cacctcgccc
      661 agcacatggc gggaggggtt gtcccggtaa agaaaggtcc acaccgcgcc cagcagcaca
      721 aagggcaggt agagcggcca gtagcagaac gcctccagca caatgccgaa ggggttttgg
      781 ggcgaataga gcgcccggtc gatggcgagg tcgtaaaagg cggccgccgc cagcagcagg
      841 gcggccagta ggcccagccc cgccagcaaa agcttttgat cttttttcag tttcacttgt
      901 gcgtcaaacc ctttcagggc cagcggcccg tgatgcgggg gcgccccttt cgcgccgccc
      961 gtctgcggcg gaaaaaacgc gtctccggca ggacacagtg taccatattt ttgccggggt
     1021 gtcgagaaac gccgaggcgc ggcgaccgcg atctcttacc caaacaaagc gagaagaaag
     1081 cagaaataag aaaaacattg caaaagtgct tttttgcacc agcttaagcg ggagaatcgc
     1141 gccggaacgc gataaccgaa agaaaagcat gataaaagca agcattgtcc tccgctgaaa
     1201 ggcgattgct ttcttttgct ttctttttag gttttttgat aataaaaaaa cagagaaagc
     1261 aatatttggc aatttgctgc gctttttttc gtaaatttgt ttaaaacaaa tataaaatgt
     1321 tggaaaaatt agggtgaaaa ctttcagaga ggaaaaaatt tgtagactta tcaaaaattg
     1381 aaaaaaattg ttctgcaaaa atgcacaaag aaaaagaaag cgaacaaaaa cggtcgattt
     1441 ggcagggccg gggcatttga ttatgataaa gccatcgagc ttccccggca gacgggggag
     1501 catgaacccc gacaacagga taaaaaggag gatacgaatg aaaaagttat cactgcttct
     1561 ggccgtttcc atgctgatga ctctgctggc cggttgcggc ggcggcgcct cgtccggcag
     1621 cggcggccca tcgtccggct cgtctccggg cgcgggcact tcccaggcgg ccgcaggggc
     1681 ctacgtcatc gcaaacaccc caaaatgcgt gggcatctcc tggtgggacc gcatgctggt
     1741 gggcaacgac cgcttcgtgg cgtccaccgg caacgaggtg taccaggtgg ggcccgcagg
     1801 gcagtccgac acggcggtac aggtctccac catcgaggat gtgatcgcca ccggcgtgga
     1861 cgcgatcacc gtcatcccct cggacccgga cgccgtggag acgaccctgg ccaaggcccg
     1921 ggagcagggc atcgtggtca tcagccacga ggcggagggc atgcagaacg tggactacga
     1981 tctggaggca tttgtcaacc gggagtacgg cgcgcacatg atggacgtgc tcgccaagga
     2041 gatgggcgag gagggcggct actgcatcat gatgggcatg ctgacgatgt cctcccatca
     2101 gcagtggacc gagggcgcca tcgagcgcca gaaagaagct tatcccaaca tgtacatggt
     2161 ctgcgatccg gtggaatccc aggcgggcgg cggctcgcag gagggctcct acaaggtggc
     2221 caaggaggtc atcgcctctt acccggacgt ggcgggcttt atcggctgtg atatggtgaa
     2281 ccctccgggc attgccatgg cggtggaaga ggccggcaag gcgggggaga tcgcggtgac
     2341 gggcacctgc ctggtatcgg ttgcgggcga atatc
//
LOCUS       DBMDYA010000473         2370 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9520_length_2370_cov_1.260026, whole genome shotgun sequence.
ACCESSION   DBMDYA010000473 DBMDYA010000000
VERSION     DBMDYA010000473.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_2370_cov_1.260026"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(155..400)
                     /locus_tag="ACZPFK_10755"
     CDS             complement(155..400)
                     /locus_tag="ACZPFK_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406835.1"
                     /translation="MRRFANPSTARLTDWAVAERLEARTGASRQDGRGTVSGQKSTGQ
                     PVDSPRRRWAVFGWLGALTALHDETGGGAVFGVAATA"
     gene            complement(731..>2370)
                     /locus_tag="ACZPFK_10760"
     CDS             complement(731..>2370)
                     /locus_tag="ACZPFK_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1406836.1"
                     /translation="AHIRRGDWLVKMAAFGAVCLHWFNPLVWLAFSLACRDMEMSCDE
                     RVLRELGDGVRAEYSRSLLVMASGRRIWNGGPLAFGENDAKSRIKNVLNFRRPAFWVM
                     VAAVAAVAALAVGLALDPAAAPASLRWAQGLDAAEVERIELLVEPSAPEQRYHLFTAE
                     ELPGVVEHIRQSRGRLVKEPEGLAGGGMTLTVTMKDGSVHTVANNANTYLVIDDESFD
                     AGYDWLSGWDYRGDALAPEGGGPSYQLDQLEKNIRYDPASGTLSLTIPAGLDTDALSL
                     QVGGRLTAGGGMSWHAFEEESAAGGWAAGKTYTAQVDADEVESISISAVLDSAARDWV
                     IAPPDFEVRVEALGAGEDEGPQDTGGLSFPHPMGGTAVTLADGGEAEVQLVMTSGKLW
                     TEDDPQFAAGGPLHAGEENYLGEFEIVVSRGGLTLSSMPFVPATGDTACFYGPVTLEV
                     MDYNDDQDPDFALGTYLWSGGNTFNLYSVDREGTLYQIGTVERHDEVAPSVLFGVPAN
                     GGHLVTLQWNQEKGDYDYLYYHYDAEQKLFLPEEPDA"
BASE COUNT          369 a          902 c          719 g          380 t
ORIGIN      
        1 gccccagccg ctcaaacata gcccgacgga cggggcggga ctgcccacgg ccatcgaggc
       61 ggccttcgcc ctgacgcggt accgccgccg cttgtcccgc cgtggggcgc tcgtcggtgc
      121 gccccagccg ctcgagcacg gcccgaccag cgggttatgc ggtggcggcc acaccaaaca
      181 ccgcacctcc acccgtctcg tcgtgaagcg ccgtcaacgc gcccagccac ccaaatacgg
      241 cccagcggcg gcgcgggctg tcgacaggct gtccagtcga cttttgtccc gacacggtgc
      301 cccggccgtc ctgtcgtgag gcgcccgtcc gcgcctccag ccgttccgcc acggcccaat
      361 cggtgaggcg ggctgtcgac ggatttgcaa atcgacgcac acatcactcc cccagccgct
      421 ccaccccagg gacggccgcc cgggcgcttt tccgcctctg tgggcgctcc ccccacccca
      481 cagggccagg tcctccgcca ggctcgccta ttttttcggc caaacctttt ctcagaccaa
      541 gcctttcacc acgcctatgc ctccgccgcg gggcgctgtg acacaaaaaa cggggccgcc
      601 cgggcgcggg gaaaacctct gttttctccc cggcccgggc cgtcccgttt ttctgtaaag
      661 ccgtgcccac ccccgcgcgg cgcctcgccc tgtcggggca acgcagcgcc gtttctcttt
      721 tcagagcgcg ctaggcatcc ggctcctcgg gcaaaaagag cttctgctct gcgtcatagt
      781 gataatacag gtaatcataa tcgccttttt cctggttcca ctgcagggtg acgagatgcc
      841 ctccgttggc gggcacgccg aacagcaccg acggggcgac ctcgtcgtgc cgctccaccg
      901 tgccgatctg gtaaagcgtc ccctcgcggt cgacactgta gaggttgaac gtgtttccgc
      961 cgctccacag ataggtcccc agcgcaaagt ccgggtcctg atcgtcgttg taatccatga
     1021 cctccagggt caccggcccg taaaagcagg cggtgtcgcc ggtggcgggc acaaagggca
     1081 tgctggagag ggtgagcccg ccgcgggaga ccacgatctc aaattcgccg agatagtttt
     1141 cttcccccgc gtgcagcggc ccgcccgccg caaactgggg gtcgtcctcg gtccagagct
     1201 tgcccgaggt catcaccagc tgaacctccg cctcgccgcc gtccgccagc gtcaccgccg
     1261 tgccgcccat gggatgcgga aagctgaggc ccccggtgtc ctgcggcccc tcgtcctccc
     1321 ccgcgcccag ggcctcaacc cgcacctcaa aatccggcgg ggcgatcacc cagtcgcggg
     1381 cggcgctgtc caggaccgcg ctgatggaaa tgctctccac ctcatcggcg tccacctggg
     1441 cggtataggt cttgcccgcc gcccagccgc ccgccgcgct ttcctcctca aaagcgtgcc
     1501 agctcatgcc gccgcccgcg gtcagcctgc cgcccacctg caggctgagc gcatcggtat
     1561 ccagccccgc cgggatggtg agggacagcg tgccgcttgc cgggtcgtac cggatgtttt
     1621 tttccagctg atccagctgg taagagggcc cgccgccctc cggcgcgagg gcgtcgcccc
     1681 ggtagtccca gcccgagagc cagtcatacc cggcgtcaaa gctctcgtcg tcgatcacca
     1741 gataggtgtt ggcattgttc gccaccgtgt gtacactgcc gtctttcatg gtcacggtga
     1801 gggtcatgcc gccgccggca aggccctccg gctctttcac cagccgcccg cggctctgcc
     1861 ggatgtgctc caccacgccg ggcagctcct cggcggtgaa cagatgatac cgctgctccg
     1921 gcgcggaggg ctccacaagc agctcgatgc gctccacctc ggccgcgtcc aggccctggg
     1981 cccagcgcag cgacgcgggc gcggcggcgg ggtcgagggc aaggcccacc gccagcgcag
     2041 cgacagccgc gacggcggcc accatcaccc aaaacgcggg gcggcggaag ttgagcacgt
     2101 ttttgatgcg gctcttggcg tcgttttctc caaaggccag cggcccgccg ttccagatgc
     2161 ggcggcccga ggccatgacc agcagcgagc gggaatactc cgcccgcacc ccatcgccca
     2221 gctcgcgcag cacccgctcg tcgcaggaca tctccatgtc ccggcaggcc agtgaaaagg
     2281 ccagccagac cagtgggttg aaccagtgca ggcagaccgc cccaaaggcg gccatcttca
     2341 ccagccagtc cccgcgccgg atgtgcgccc
//
LOCUS       DBMDYA010000474         2362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9557_length_2362_cov_1.490491, whole genome shotgun sequence.
ACCESSION   DBMDYA010000474 DBMDYA010000000
VERSION     DBMDYA010000474.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_2362_cov_1.490491"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="ACZPFK_10765"
     CDS             complement(<1..1451)
                     /locus_tag="ACZPFK_10765"
                     /EC_number="3.6.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1406837.1"
                     /translation="MLIKYKAGDVAKDLGQPNKELLEVVTKKFGEGKKYTTPLTEQEL
                     NYVFEHYTKTKQVASFDEYLASAPQPKPAAPAEPLKKADGTVVELKRTGGSNIDFKAP
                     PKKEKPAQPAQPKREVVTVRVDTRGGDVNLDKFNEKYESLAGAPTRGAAQASGNGKHG
                     KQKVGPGGKQKFAQRNNRRGQPFKRRETEAERLQRLQLEKARKAQLKVSIPDEITVGE
                     LASRLKQTASNVIKKLMGLGVMASVSEVVDYDTAALVADEFGAKVEHEVHVTIEERLF
                     EDVADDPEALEERPPVVVVMGHVDHGKTSILDAIRKTRVTAGEAGGITQAIGAYQVEA
                     GGKAITFLDTPGHEAFTSMRARGADLTDIAVLVVAADDGIMPQTVESINHAKAAGTTI
                     IVAINKMDKPQANPEQVKEQLTKYELVPEEWGGETICVPVSALTGQGIPELLENINLL
                     AEVKELRANPNRRAKGAVVEARLDKGQGPVATIL"
     gene            complement(1476..>1749)
                     /locus_tag="ACZPFK_10770"
     CDS             complement(1476..>1749)
                     /locus_tag="ACZPFK_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406838.1"
                     /translation="PVAESVAKGKAQLVLLAADLAEGSRKKALRFCDAAGLEPRALPL
                     TQQELLAVTPKRTGVFAVIDKGLAQLVERQLAPAGPGGGNPSPAEH"
     assembly_gap    1750..1759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1814..2098)
                     /gene="rnpM"
                     /locus_tag="ACZPFK_10775"
                     /gene_synonym="ylxR"
     CDS             complement(1814..2098)
                     /gene="rnpM"
                     /locus_tag="ACZPFK_10775"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1406839.1"
                     /translation="MVTKKVPVRRCVGCNEGKPKKELVRIVRSAEGEISVDVTGKKSG
                     RGAYICPSRDCLAKAQKARRLERAFSCAIPPEIYERLAAEVEQRAEEADG"
     gene            complement(2138..>2362)
                     /locus_tag="ACZPFK_10780"
     CDS             complement(2138..>2362)
                     /locus_tag="ACZPFK_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1406840.1"
                     /translation="ISAALSPATVVKVEILDGEAKSCKVTVPDNQLSLAIGNKGQNAR
                     LTARLTGYNIDIRPESGYYGEEPEAAPAAE"
BASE COUNT          290 a          815 c          762 g          485 t
ORIGIN      
        1 agaatggtgg ccacggggcc ctggcccttg tccaggcgcg cctcaacgac cgcgcccttg
       61 gcgcggcggt tggggttggc ccgcagctct ttgacctcgg ccagcagatt gatgttctcc
      121 agcagctcgg ggatgccctg cccggtgagg gcggagaccg gcacacagat ggtttcgccg
      181 ccccactcct cgggcaccag ctcgtatttg gtgagctgct ccttgacctg ctcggggttt
      241 gcctggggtt tatccatctt gttgatggcc acgatgatgg tggtgcccgc cgccttggcg
      301 tggttgatgg actcgacggt ctggggcatg atgccgtcgt ccgccgccac caccagcacc
      361 gcgatatcgg tcaggtcggc gccgcgggcg cgcatggagg tgaaagcctc atggcccggg
      421 gtgtccagga acgtgatcgc cttgccgccg gcctccacct gataggcgcc gatggcctgg
      481 gtgatgccgc ccgcctcgcc cgcggtgacg cgggtcttgc ggatggcgtc caggatggag
      541 gtcttgccgt ggtccacgtg gcccatgacc accaccaccg gcgggcgctc ctcgagggcc
      601 tccgggtcgt cggccacatc ctcaaacagg cgctcctcga tggtgacatg cacctcgtgc
      661 tccaccttgg cgccgaactc atccgccacc agggcggccg tgtcgtagtc caccacctcg
      721 gacaccgagg ccatcacgcc caggcccatc agctttttga tgacgttgga cgcggtctgc
      781 ttgaggcggg aggccagctc gcccaccgtg atctcgtcgg ggatcgagac tttcagctgg
      841 gccttgcggg ccttttccag ctgcaggcgc tgcaggcgct cggcctcggt ctcgcggcgc
      901 ttgaacggct ggccgcgccg gttgttgcgc tgggcgaact tctgcttgcc gcccggcccc
      961 accttctgct tgccgtgctt gccgttgccc gaggcctgcg ccgcgccgcg ggtgggcgcg
     1021 ccggccaggc tctcgtattt ctcgttgaat ttgtccaggt tgacgtcgcc gccgcgggtg
     1081 tccacccgca cggtcaccac ctcgcgcttg ggctgggcgg gctgggcggg cttttccttt
     1141 ttcggcgggg ccttgaagtc gatgttgctg ccgccggtgc gcttgagctc caccacggtg
     1201 ccgtccgctt ttttcagcgg ctccgccggc gcggcgggct tgggctgcgg ggcgctggcc
     1261 agatactcgt cgaagctggc cacctgcttc gtcttggtgt agtgctcaaa gacgtagttc
     1321 agctcctgct ccgtgagggg ggtggtatat tttttgccct ccccaaattt ttttgtgacg
     1381 acctccagca gctctttgtt gggctgcccc aggtctttgg cgacgtcgcc ggctttgtat
     1441 ttaatcagca tataagcagt acctccatcg gttggttaat gttcggcggg ggacgggttg
     1501 ccgccccccg gcccggccgg ggcgagctgg cgttccacca gctgcgcgag gcctttatcg
     1561 atcacggcga agacgccggt cctttttggt gtgacggcca gcagctcctg ctgggtgagg
     1621 ggcagcgcgc ggggctcgag gcccgcggcg tcacaaaagc gcagcgcctt tttgcggctg
     1681 ccctccgcca gatcggcggc cagcagcacc agctgcgcct tgccctttgc cacgctctcc
     1741 gccaccgggn nnnnnnnnna agcccatcac cagcttgccc gccttgcggc agagcgacag
     1801 cgcgccaagc agcttatcca tccgcttcct ccgcgcgctg ctccacctcg gcggcgagcc
     1861 gctcgtagat ctcgggcggg atcgcgcagc tgaacgcccg ctcgaggcgc cgcgctttct
     1921 gcgccttggc cagacagtcg cggctcgggc agatataggc cccgcgcccc gactttttgc
     1981 cggtcacatc cacgctgatc tccccctcgg cgctgcgcac gatgcgcacc agctcctttt
     2041 tgggcttgcc ctcgttgcag ccgacacacc ggcgcaccgg gacctttttt gtcaccatct
     2101 tgagcatccc tccgtcctgc cgtaaccccg ccccgcgcta ctcggccgcg ggggccgctt
     2161 caggctcctc gccgtaatag ccgctctcgg ggcggatatc gatattgtac ccggtcagcc
     2221 gcgcggtcag ccgcgcgttc tggcccttgt tgccgatggc cagcgagagc tggttgtcgg
     2281 gcacggtcac cttgcagctc ttggcctcgc cgtccaaaat ttccactttc accaccgtgg
     2341 ccggcgagag ggcggcggag at
//
LOCUS       DBMDYA010000475         2362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9562_length_2362_cov_1.254755, whole genome shotgun sequence.
ACCESSION   DBMDYA010000475 DBMDYA010000000
VERSION     DBMDYA010000475.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9562_length_2362_cov_1.254755"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="ACZPFK_10785"
     CDS             <1..970
                     /locus_tag="ACZPFK_10785"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1406841.1"
                     /translation="LEMAGKDPAAVIGGKLPLIHGYGKAGKGENIVVESCEFSNTFLH
                     LSPDTSILLNIDADHLDFFKTMENLKASFRKFAELTQRRIIANGDDANTVETLAGIGR
                     EVVTFGQTEGCDYIAKNIRMVRPAFYGYEVWHRGERLAEVTLSVPGKHNVYNSMAAFV
                     ASTLAGCTAAEAVRGIDAFTGTGRRFEVLGHTACGATVADDYAHHPTELEATLSAAKQ
                     MGFERVIAVFQPFTYSRTKLLLKEFARVLQIADQVVMSEIMGSRETNDAFNIYTADLA
                     ALIPGSVWFPGFDGIVDYIEKNAKKGDLVITLGCGDIYKAAKRMIV"
     gene            complement(1325..2026)
                     /locus_tag="ACZPFK_10790"
     CDS             complement(1325..2026)
                     /locus_tag="ACZPFK_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR1406842.1"
                     /translation="MSEEKRRPGACLMVAIVDRGKGEKAALLCGGRRAPHTICPAQGT
                     ANSEILDVLGLARTSKDLVLGLLPPGAAQGAPLRLAQGMELTRPGRGIAFTLPLSGAS
                     SRAVRLAQEGDFETREEGSERPMGEQMHYDLILAIVNEGCADLAVDATTAAGARGGTL
                     LHGRGTGDAAGFLGVPLPPEREIVAILVPASLRHAVLASVNAAAGLSSEAQGLVFSLP
                     VDEVAGLHPAAGGLD"
     gene            complement(2019..>2362)
                     /locus_tag="ACZPFK_10795"
     CDS             complement(2019..>2362)
                     /locus_tag="ACZPFK_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HIR1406843.1"
                     /translation="VSIFWFLIPGYALALGLMRFVPPIFTGIAFDSGGVASGPMTATF
                     LLPFAMGACESLGSNLLTDAFGVVAMVAMTPLVTIQLMGFVYSRRANRAAGQTEGDEI
                     VEYDCGEVQYE"
BASE COUNT          430 a          811 c          696 g          425 t
ORIGIN      
        1 cctggagatg gcgggcaagg accccgccgc agttatcggc ggcaaattac cgctgatcca
       61 cggctacggc aaggcgggca agggagaaaa catcgtggtg gagagctgcg aattctccaa
      121 caccttttta catctcagcc ccgacaccag catcctgctc aacatcgacg cggatcatct
      181 ggactttttc aagaccatgg aaaatctgaa agccagcttt cgcaaatttg ccgagttgac
      241 ccagcggcgc atcatcgcca acggggatga cgccaacacc gtggagaccc tggccggtat
      301 cgggcgtgag gtcgtcacct ttgggcagac ggagggctgc gactacatcg ccaaaaacat
      361 ccgcatggtg cgccccgcgt tttacggcta cgaggtctgg caccggggcg aacgccttgc
      421 cgaggtgacc ctgtcggtgc ccggcaagca caatgtctac aactccatgg ccgcctttgt
      481 ggcgtccacc ctggcgggct gtaccgccgc tgaggctgtg cgcggcattg atgctttcac
      541 aggcactggg cgccgctttg aggtgttggg gcacaccgcc tgcggggcca cggtggcgga
      601 tgattacgcc caccacccca ctgagctgga ggccactctc tccgccgcca agcagatggg
      661 ctttgagcgg gtgatcgctg tgttccagcc gttcacctac tcccgcacca agctgctgct
      721 gaaagagttt gcccgtgttt tgcaaattgc cgaccaggtg gtgatgagcg agatcatggg
      781 cagccgcgag acgaacgacg ctttcaacat ctacaccgcc gatctggcgg ccctcatccc
      841 cggcagcgtg tggttccccg gctttgacgg catcgtggat tacatcgaaa aaaacgccaa
      901 aaagggcgat ctcgtcatca ccctgggctg cggcgacatc tacaaggccg ccaagcggat
      961 gatcgtctga aaaggcgggc tcacgcgaac gcgcggcccc ggcagggaaa ggccgcctcc
     1021 gggattttga tggaaaagcc cttttctgcc gcgtcggtgg caccagcctt acaggcttgc
     1081 cagcgctatt ttttaccagc acgcttttcc gcgagcagca gctgctgtcc cgctttgcac
     1141 cgtcgggcaa gaccaagcgt gtcccaccct cgctgtgctt gccctccctg cgctcttgtt
     1201 ccagtgaaaa agtccccggc tgtggccggg ggcttttttc gtatttttcg tattttgcgt
     1261 gtaccgggcg gcgagggccg ggcaggccct ttttttcgac tgtgcccggc gccaaaaacg
     1321 ttttttagtc caggcctccc gccgcggggt gcagacccgc cacctcatcc accggcaaag
     1381 agaagaccag gccctgcgcc tcgctggaga ggcctgcggc ggcattgacc gacgccagca
     1441 ccgcatggcg cagcgacgct ggtaccagaa tcgcgacgat ctcccgctcc ggcggcagcg
     1501 gcacccccaa aaagcccgcc gcatcgccgg tgccgcgccc gtgcagcagg gttccgcccc
     1561 gggcgcccgc cgctgtggtc gcgtccacgg cgagatcggc gcagccctcg ttgacgattg
     1621 ccaggatcag gtcgtaatgc atctgttctc ccatgggtct ttcacttccc tcttctctag
     1681 tctcaaagtc gccctcctgc gccaggcgca ccgcccggct gcttgctccc gagagaggca
     1741 gggtaaaggc gatgccccgg cccggccgcg tgagctccat cccctgcgcc aggcgcagcg
     1801 gcgccccctg cgcggcacca gggggcaata accccagcac caggtctttg gaggtgcggg
     1861 ccaggccgag aacatccagt atctcgctgt tcgccgtccc ctgtgccggg cagatggtgt
     1921 ggggcgcccg cctgcccccg cacaggagcg ccgccttttc gccctttccc ctgtccacga
     1981 tcgccaccat caggcaggcg cccggccgcc ttttctcttc actcatactg cacctccccg
     2041 cagtcgtact ccacgatctc gtccccctcc gtctgcccgg ccgcccggtt tgccctgcgg
     2101 ctgtagacaa agcccatcaa ctggatggtg accagcgggg tcatggccac catggccacc
     2161 accccgaacg catcggtcag aagattgctg cccaggctct cacaggcccc catggcaaag
     2221 ggcagcaaaa aggtggccgt catcgggccg ctggccacgc ccccagagtc aaacgcgatg
     2281 ccggtgaaga tcggcggcac aaagcgcatg agccccagcg ccagcgcgta accggggatc
     2341 agaaaccaaa aaatcgaaac gc
//
LOCUS       DBMDYA010000476         2361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9567_length_2361_cov_1.491593, whole genome shotgun sequence.
ACCESSION   DBMDYA010000476 DBMDYA010000000
VERSION     DBMDYA010000476.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9567_length_2361_cov_1.491593"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="ACZPFK_10800"
     CDS             <1..271
                     /locus_tag="ACZPFK_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406844.1"
                     /translation="RQKGRLRCRSILDCGVGRDEIVKALRVALSPAFALQCKAARSPY
                     NGGDTSGMIVSLLAAFLNSALAHQPKLFYDMPACAGCKGEKDHDD"
     gene            405..1391
                     /locus_tag="ACZPFK_10805"
     CDS             405..1391
                     /locus_tag="ACZPFK_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1406845.1"
                     /translation="MKLLIVGLGSMGKRRARLLRGLEPGAELFGVDSNPGRRAEAEGM
                     GVRAFESIPAAVAARRYDAALVCTAPLSHAAIIAQLLDAGLPVFTELNLVADGYRENM
                     AKAREKGLLLFLSSTMLYRGETRHIAGRVRAFGGPVNYLYHIGQYLPDWHPWEDYKNF
                     FVGDARTGGCREIFGIELPWLLDAFGPAASVQARADRLTSLDLPYPDCWFVTLTHENG
                     ARGVLAVDVVCPKAVRSFEAFGEGLHLFWEGNPRELYEFDPEAKEKRFVPTYQTVEQD
                     PRYSDNIVENAYVDELAEFLACLRGETTARYSFEKDLATLGLIDEIERSAGQ"
     gene            1388..2314
                     /locus_tag="ACZPFK_10810"
     CDS             1388..2314
                     /locus_tag="ACZPFK_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR1406846.1"
                     /translation="MRALFVGLGSIGTRHLKNLCGRCASRGIPLQVDALRSSPRPLPP
                     QTAALLRQQFTESDKLDHYDLLFITNPTNLHFSAIRELAGKADCLFIEKPIFEDGRYS
                     LEDAGLAGKKALVAAPMRYCGAFLALKEELQGRRVYSARLICSSYLPDWRPGVDYRRV
                     YSARREMGGGVTIDLIHEWDYLVDLFGFPQQSYNLKGTYSQLEIDSDDLSVYIARYPS
                     LLAEIHLDYFGRTYRRTAEFFVQEGTITADFGAGTLTRADGSVLHCEEEVNARYLREM
                     DALLDYALGDAQVSANPPQMALQVLKLTLGEE"
BASE COUNT          395 a          741 c          807 g          418 t
ORIGIN      
        1 ccggcaaaag gggcggctgc gctgccgctc gatcctggac tgcggcgtcg gcagggacga
       61 gatcgtcaag gccctgcggg tggcgctttc gcccgccttt gcgctgcagt gcaaggcggc
      121 ccgcagcccc tataacggcg gcgacaccag cggcatgatc gtcagcctgc tggccgcctt
      181 tttgaacagc gcgctggcac atcagcccaa gctgttctac gacatgcccg cctgtgcggg
      241 ctgcaagggc gaaaaggatc acgacgactg agcgggcgca gagagggcgc gcttctgcag
      301 cccgggcggc ggggcccggc gggtgtcccc gctccgccct cttttcagcc ccggcgcaca
      361 gccgcgcccg gtcggatcat gatgagaaat gtgaggaaaa cgatatgaaa ctgctgattg
      421 tcggccttgg cagcatgggc aagcgccgcg ccaggctgct gcgcggcttg gagccggggg
      481 cggagctctt cggcgtggat tcgaaccccg gccgcagggc cgaggcggag gggatgggcg
      541 tgcgcgcctt tgagagcatc cccgccgcgg tggcggcgcg gcgctatgac gcggcgctgg
      601 tctgcaccgc gccgctctcc cacgcggcga tcatcgccca gctgctggac gcggggctgc
      661 cggtcttcac cgagctgaac ctggtggcgg acggctaccg ggagaacatg gcaaaggcgc
      721 gggaaaaagg gctgctgctc tttctctcca gcaccatgct ctaccggggc gagacccggc
      781 acatcgccgg gcgggtgcgg gcgttcgggg ggccggtgaa ctatctctac catatcggcc
      841 aatacctgcc cgactggcac ccgtgggagg attacaaaaa cttttttgtg ggcgacgccc
      901 gcaccggcgg ctgccgcgag atcttcggca tcgagctgcc ctggctgctg gacgcgttcg
      961 gcccggcggc gtcggtgcag gcgcgggccg accggctgac cagcctggac ttgccctatc
     1021 ccgactgctg gttcgtcacc ttgacccatg aaaacggcgc ccgcggcgtg ctggcggtgg
     1081 atgttgtctg ccccaaggcg gtgcgcagct ttgaggcgtt cggcgagggg ctgcacctgt
     1141 tctgggaggg caacccgcgg gagctgtacg agtttgaccc ggaggccaaa gaaaagcgct
     1201 tcgtgcccac ctatcagacg gtggagcagg acccccgtta cagcgacaac atcgtcgaaa
     1261 acgcctatgt ggacgagctg gcggagtttt tggcctgcct gcgcggcgag accacggccc
     1321 gttacagctt tgaaaaggat cttgccaccc tgggcctgat tgacgagatc gagaggagtg
     1381 cagggcaatg agggcgctgt ttgtgggcct gggctccatc ggcacgcggc atttgaaaaa
     1441 cctgtgcggg cgctgcgcct ctcgcggcat ccccctgcag gtggacgcgc tgcgctcctc
     1501 gccgcgcccg ctgccgccgc agacggcggc gctgcttcga cagcagttta cagagagtga
     1561 taaactggac cattatgacc ttttgtttat cacaaacccc accaatctgc acttttccgc
     1621 catccgcgag ctggcgggca aggcggattg cctttttatc gaaaaaccga tttttgagga
     1681 cggccgctac agtttggagg acgcgggcct ggccggcaaa aaagcgctgg tggccgcccc
     1741 catgcgctac tgcggcgcct ttttggcctt gaaagaggag ctgcagggca ggcgggtcta
     1801 ctcggcgcgg ctgatctgct ccagctatct gccggactgg cggcccggcg tggactaccg
     1861 ccgggtctat tcggcccggc gggagatggg cggcggggtg accatcgacc tgatccacga
     1921 gtgggactat ctggtggacc tgttcggctt cccgcagcag agctataatc tcaaggggac
     1981 ctattcgcag ctggagatcg acagcgacga cctgtcggtc tatatcgccc gctatccctc
     2041 gctgctggcc gagatccatc tggattactt cggccgcacc taccggcgca ccgcggagtt
     2101 ctttgtgcag gagggcacca tcacggcgga tttcggcgcc ggcaccctca cccgcgccga
     2161 cggcagcgtg ctgcactgcg aggaggaggt caacgcccgc tatctgcggg agatggacgc
     2221 gctgctggac tatgccctgg gggacgcaca ggtgagcgcc aacccgccgc agatggcgct
     2281 gcaggtgctg aagctgacgc tgggcgagga gtagctttgc ccgttttaag tgaaaagctc
     2341 tcctgcttta caccgtgaaa a
//
LOCUS       DBMDYA010000477         2357 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9583_length_2357_cov_1.184397, whole genome shotgun sequence.
ACCESSION   DBMDYA010000477 DBMDYA010000000
VERSION     DBMDYA010000477.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9583_length_2357_cov_1.184397"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="ACZPFK_10815"
     CDS             complement(<1..1980)
                     /locus_tag="ACZPFK_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR1406847.1"
                     /translation="MRTEAFEILAPAGNAEMLKAAVFAGASSVYLGLTGFNARRTAGN
                     FTPQALAQAVAFCHARDVKVNVTLNTLCYDGELEGLRAAVAAIAASGADAVIVQDLAA
                     AELVRASAPGLALHGSTQMSVMNLAGARQLAEMGFSRVILARELTAQEIKRITAGCGI
                     ETEIFVHGALCMSVSGQCLMSAFYGGRSGNRGGCAGPCRLPYRLDGQEGHFLSLKDLS
                     LIDRLPEVREMGVRCAKIEGRLRTPEYVAAAVSAARAALEGRAYDRALLEKAFSRAGF
                     TSGFYDNAYLSEKMFGRRDEAQAADTKAALPRLRELYRRERPSVPVSLSLTLEDEGAK
                     LAAADRDGNRAVAYSETVPEPAQRPEQALREGYEKALDKTGGTPFFAEKIEVAGAAGR
                     YLPASAVNELRRQALAALLQKREAPRPWPYAGQPLPQAGRAPAEPAAQRLFLRFCTPQ
                     QCGEALAGLGDRLIFPLGCAAELPAAWREKTVLALPRALFDEELVAAQLRRAAGLGFA
                     AFEIENIGGLPLVRENAPGAKILGGFGLNVTNGAAARRYAGLGLAALTLSPELPLAAA
                     NRLGAGVPTGLLAYGHLPLMLTRACPVRNRKTCAECRAQGVLVDRKGRRLTVACTGPG
                     GDGARELLNPVPLWLGDRRREVGTDFTVLYFTGESA"
     gene            complement(1970..>2357)
                     /locus_tag="ACZPFK_10820"
     CDS             complement(1970..>2357)
                     /locus_tag="ACZPFK_10820"
                     /inference="COORDINATES: protein motif:HMM:NF017016.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1406848.1"
                     /translation="TIHLQRKEVLAMPVNKIRLEICGCSYLISTTDSEQYTLSLAEKL
                     DHNMNEIMAQNPSASVTAAAVLSALDYLDELEKSSASADNMRSQIKDYLEDAAKAKLE
                     AEEAKKEIERLRREIGYLKGSAAGEN"
BASE COUNT          330 a          892 c          772 g          363 t
ORIGIN      
        1 ggcgctctcg ccggtgaagt agagcacggt gaaatccgtg cccacctcgc gcctgcggtc
       61 gcccagccag agcggcaccg ggttgagcag ctcgcgggcg ccgtccccgc ccgggccggt
      121 gcaggccacc gtcagcctgc ggcccttgcg gtccaccagc accccctggg cccggcactc
      181 ggcgcaggtc ttgcggttgc gcaccgggca ggcccgggtc agcatcagcg gcagatggcc
      241 gtaggccagc aggcctgtgg ggacccccgc ccccaggcgg ttggccgcgg ccagcggcag
      301 ctcgggcgag agggtcaggg ccgccaaccc cagcccggca tagcggcgcg cggccgcgcc
      361 gttggtcacg ttgaggccga agccgcccag gatttttgcc cccggcgcgt tttctcgcac
      421 cagcggcagg ccgccgatgt tctcgatctc gaacgcggcg aagcccagcc cggccgcccg
      481 ccgcagctgc gcggccacca gctcctcatc aaagagggcc cggggcaggg ccagcacggt
      541 tttttcccgc caggcggcgg gcagctccgc cgcgcacccc agcgggaaga tcagccgatc
      601 ccccagcccg gccagcgcct ccccgcactg ctgcggggtg caaaaccgca aaaaaagccg
      661 ctgcgcggcc ggctcggccg gcgcccggcc cgcctggggc aggggctgcc cggcataggg
      721 ccaggggcgg ggcgcctcgc gcttttgcag cagcgcggcc agcgcctgcc gccgcagctc
      781 gttcacggcg gaggcgggca gatagcgccc ggccgccccg gcgacctcga ttttctcggc
      841 aaaaaagggg gtgccgccgg tcttgtccag cgccttttca tagccctccc gcagggcctg
      901 ctccggcctt tgggcgggct cgggcacagt ctctgaatag gccaccgccc ggttgccgtc
      961 ccggtcggcc gccgccagct tggcgccctc gtcctccagc gtgagggaga gcgacacggg
     1021 caccgacggc cgctcgcgcc ggtagagctc ccgcaggcgc ggcagcgccg ccttggtgtc
     1081 cgcggcctgc gcctcgtccc tgcggccgaa catcttttcg ctgagatagg cgttgtcgta
     1141 aaagccggag gtgaagcccg cgcggctgaa cgccttttcc agcagcgccc ggtcataggc
     1201 ccggccctcc agcgccgccc gcgccgcgct caccgcggcg gcgacatact ccggcgtgcg
     1261 cagccgcccc tcgatcttgg cgcagcgcac ccccatctcc cgcacctcgg gcagccggtc
     1321 gatcagcgag aggtctttca ggctcaaaaa atgcccctcc tggccgtcca gccggtaggg
     1381 caggcggcag ggcccggcgc agccgccccg gttaccgctg cggccgccgt agaacgcgct
     1441 catcaggcac tggccggaca ccgacatgca cagcgcgccg tgcacgaaga tctctgtctc
     1501 aatgccgcag cccgcggtga tccgctttat ctcctgcgcg gtcagctccc gcgccaggat
     1561 gacccgggaa aagcccatct ccgccagctg gcgcgcgccg gccaggttca tcaccgacat
     1621 ctgggtggag ccgtgcagcg ccagcccggg ggccgaggcg cgcaccagct cggccgccgc
     1681 caggtcctgc acgatcaccg cgtccgcgcc cgacgccgcg atggccgcca cggcggcgcg
     1741 caggccctcc agctcgccgt catagcacag ggtgttcagg gtcacgttca ctttcacgtc
     1801 ccgcgcgtgg cagaaagcca ccgcctgcgc cagggcctgc ggggtgaaat tgcccgccgt
     1861 gcgccgtgcg ttgaagccgg tcagccccag atagacgctg ctcgcgccgg caaacaccgc
     1921 cgctttgagc atttcggcgt tgcctgccgg cgcgaggatc tcaaacgctt cagttctcac
     1981 cggccgccga ccctttcagg tagccgatct cccggcgcag ccgctcgatc tccttttttg
     2041 cttcctccgc ctccagcttc gcctttgcgg cgtcctccag ataatccttg atctggctgc
     2101 gcatgttgtc ggccgaggcg ctggattttt ccagctcgtc cagatagtcc agcgccgaga
     2161 gcaccgccgc cgccgtcacc gacgcggagg ggttctgcgc catgatctcg ttcatgttgt
     2221 ggtccagctt ctccgcgagg gagagggtgt actgctcgct gtccgtggtg gagatcaggt
     2281 agctgcagcc acagatttcc agcctgattt tattcaccgg catcgccaaa acctctttcc
     2341 gttgcaaatg gatggtc
//
LOCUS       DBMDYA010000478         2354 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9599_length_2354_cov_1.367510, whole genome shotgun sequence.
ACCESSION   DBMDYA010000478 DBMDYA010000000
VERSION     DBMDYA010000478.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9599_length_2354_cov_1.367510"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="plsY"
                     /locus_tag="ACZPFK_10825"
     CDS             complement(<1..622)
                     /gene="plsY"
                     /locus_tag="ACZPFK_10825"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR1406849.1"
                     /translation="MLFLAAILSALCAYLLGSLNFAIIVSRLFFHKDIRQFGSGNAGM
                     TNILRTFGKGAAAGVLCGDVLKGTCAVLLGRLFFTLLAPGAGVIYGAYIAAIAAVLGH
                     LFPLYFGFKGGKGISVATGAIFAIEPAVILALIAVFLVMVVCTRIVSLSSITVAALYP
                     VFTFVYFTFWGDGSSLPWVNTLFAAVMGALIIYMHRANIQRLRNGTE"
     gene            complement(714..2039)
                     /gene="der"
                     /locus_tag="ACZPFK_10830"
     CDS             complement(714..2039)
                     /gene="der"
                     /locus_tag="ACZPFK_10830"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1406850.1"
                     /translation="MAKPIVAIVGRPNVGKSTLFNKLVGQRLSIVEDTPGVTRDRIYG
                     TCEWQDRQFLLVDTGGIEPDNDAGLLGHIRRQAELAIETAQCIIFVTDLRGGVTASDE
                     DIARLLMRSGKPVVLAVNKCDALGEPPIELYDFYALGLGEPYPVSSVHGHGTGDLLEE
                     VCGHLTFEDEDAQESDRIPVAVIGRPNVGKSSLVNHILGEKRMIVADEAGTTRDAIDA
                     EVDNQYGSFTFIDTAGLRKKGRVEDGVERYAVIRSLAAVERARVCVILIDGTVGFTEQ
                     DSKVAGFAHEQGKACIIAVNKWDAVEKDDKTMDATRKKLMDDFSFMSYAPIIFISAKT
                     GQRVDRLFELIQYVDSQNALRITTGMLNDLLARATARVQPPSDKGKRLKIFYMTQAST
                     RPPTFVAFVNSRQLFHFSYQRYLENQIRETFGLEGTPVRLLIRERGEGR"
     gene            complement(2101..>2354)
                     /locus_tag="ACZPFK_10835"
     CDS             complement(2101..>2354)
                     /locus_tag="ACZPFK_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1406851.1"
                     /translation="NNFFGGNVTVTGLLTGQDIAAQAAGQMLSHRLLLPGTTLRSEQD
                     ILLDDMSVEELGARLGARVTVIPQDGAAFARAALGLPPG"
BASE COUNT          446 a          779 c          727 g          402 t
ORIGIN      
        1 attcggtgcc gttgcgcagc cgctgaatgt tcgcccggtg catatagatg atgagcgcgc
       61 ccatcaccgc tgcaaacagg gtgttgaccc agggcaggct ggacccgtcc ccccagaaag
      121 tgaaatacac aaaggtgaac accgggtaga gcgccgccac cgtgatggaa gacagagaga
      181 cgatgcgggt gcagacgacc atgaccagaa agaccgcgat cagggccagg atgacggcgg
      241 gctcgatggc gaagattgcg ccagtggcca ccgagatgcc cttgccgccc ttaaagccga
      301 aatagagcgg gaacagatgc cccagcaccg cggcgatggc ggcgatgtag gcgccgtaga
      361 tcacccccgc ccccggggcc agcagggtga agaacagtct gcccagcaat accgcacagg
      421 tgcccttgag cacgtcgccg cagagcacgc ctgcggcggc ccccttgccg aacgtgcgca
      481 ggatgttggt catgcccgcg ttgccgctgc cgaactgccg gatgtccttg tgaaaaaaca
      541 gccgcgagac gatgatcgca aaattgagcg agccgagcaa ataggcacag agcgccgaca
      601 ggatcgccgc gagaaacagc atgagcttcc cctccccttg cgcggggccg cagcgcccgg
      661 cgcgtatgag aaaattttac cggctctact ttaccacatc cggccgcaaa agtctaccgc
      721 ccctcgcccc gctcgcggat cagcagccgc accggcgtgc cctccaggcc aaaggtctcg
      781 cggatctggt tttcgagata gcgctgatac gaaaaatgga acagctgccg cgaattgaca
      841 aaggccacaa acgtgggcgg ccgggtggag gcctgggtca tgtaaaaaat cttcagccgc
      901 ttgcccttgt ccgagggcgg ctgtacccgg gcggtggccc gcgccagcag gtcgttgagc
      961 atgccggtgg tgatgcgcag ggcgttctgg ctgtccacat attggatgag ctcaaacagc
     1021 cggtccaccc gctggccggt cttggcgctg ataaagatga tcggcgcata ggacatgaag
     1081 ctgaaatcat ccatcagctt tttgcgcgtc gcgtccatgg tcttgtcgtc cttttccacc
     1141 gcatcccact tgttgaccgc tatgatacag gccttgccct gctcatgggc gaagcccgcc
     1201 accttggaat cctgctcggt aaagcccacg gtgccgtcga tcaggatgac gcagaccctg
     1261 gcccgctcca ccgccgccaa gctgcggata accgcatacc gctccacccc gtcctccacg
     1321 cggccctttt tgcgcaggcc cgccgtgtcg ataaaggtga agctgccgta ctggttgtcc
     1381 acctcggcgt cgatggcgtc gcgggtggtg cccgcctcgt cggccacgat catgcgcttt
     1441 tcgcccagga tatggttgac caggctggac ttgcccacat tggggcggcc gatgaccgcc
     1501 accgggatac ggtcggactc ctgcgcgtcc tcgtcctcaa aggtcaggtg gccgcacacc
     1561 tcctccagca ggtcgccggt gccgtgcccg tgtacggagg agacgggata gggctcgccc
     1621 agacccagcg cgtaaaaatc gtacagctct atgggcggct cgcccagcgc gtcgcatttg
     1681 ttcaccgcca gcaccaccgg cttgccgctg cgcatcagca gccgcgcgat atcctcgtcg
     1741 ctggccgtca caccgccgcg gagatcggtg acaaagatga tgcactgggc ggtctcgatg
     1801 gcaagctccg cctgccggcg gatgtggccc agcagccccg cgtcgttgtc gggctcgatg
     1861 ccgccggtgt ccaccagcaa aaactgccgg tcctgccatt cgcaggtgcc gtagatacga
     1921 tcccgcgtga cgccgggggt gtcctccacg atggagaggc gctggccgac cagcttgttg
     1981 aacagggtcg atttgcccac gttggggcgg ccgacaatgg ccacaatcgg ttttgccata
     2041 gcttttctaa ctcctttcgc cgcccgcagg gcagccgaaa aacgatcttg tgaaaggggc
     2101 tcaccctggc ggcagcccca gcgccgcgcg cgcgaacgcc gcgccgtcct gcgggatgac
     2161 ggtcacccgg gcgcccagcc gcgcgcccag ttcctccacc gacatatcgt ccagcaggat
     2221 atcctgctcg ctgcgcagcg tggtgcccgg cagcagcagg cggtgcgaca gcatctgtcc
     2281 cgccgcctgg gccgcgatgt cctgcccggt caaaaggccg gtcacagtga cgttaccgcc
     2341 gaaaaagttg tttt
//
LOCUS       DBMDYA010000479         2350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9610_length_2350_cov_1.951534, whole genome shotgun sequence.
ACCESSION   DBMDYA010000479 DBMDYA010000000
VERSION     DBMDYA010000479.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9610_length_2350_cov_1.951534"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(151..660)
                     /locus_tag="ACZPFK_10840"
     CDS             complement(151..660)
                     /locus_tag="ACZPFK_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1406852.1"
                     /translation="MSVFCPDYIFKSVDRITPEFLRRRGITALVLDVDNTLTAHDSQE
                     LAPGVARWLDTMRHSGIEMRISSNNVARRVRPFAEKVGLPFVSFSLKPASRGLRLARR
                     AFGVEKGRMALVGDQIFTDTLAAKLYGVTMLLVQPVAPDTKWNIRLKRVLERPFLARY
                     YKKGGRLYE"
     gene            complement(807..983)
                     /gene="rpsU"
                     /locus_tag="ACZPFK_10845"
     CDS             complement(807..983)
                     /gene="rpsU"
                     /locus_tag="ACZPFK_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1406853.1"
                     /translation="MSEIRVKDNESLDSALRRFKRSCARSGVLAEVRKRECYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(1174..>2350)
                     /locus_tag="ACZPFK_10850"
     CDS             complement(1174..>2350)
                     /locus_tag="ACZPFK_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1406854.1"
                     /translation="TSFKLRDWVFSRQRYWGEPIPMVYCEKCGWQPLPESELPLKLPE
                     VQNYEPTEDGESPLAHLTDWVNTTCPCCGGPARRETDTMPQWAGSSWYFLRYCDPHND
                     QAIASPEALNYWMPVDWYNGGMEHTTLHLLYSRFWHKFLYDIGVVPTAEPYAKRTSHG
                     MILGENGEKMSKSRGNVVNPDDIVDQYGADTMRLYEMFIGDFEKAAPWSSSSIKGCKR
                     FLERVFDLSERLLPGEGYRPQLETSFHKAIKKVGEDILQLKMNTAIATLMSLLNEVDD
                     AGGVTRDEYRTFLLLLNPFAPHITEELWQKAGFEGQIAHAAWPVYDEDKCKDAQIELA
                     VQVCGKVRGHFVAAADVSAAQAIAAAKQAEGVAALLEGKTVVKEIYVPGKLVNLVVR"
BASE COUNT          431 a          735 c          706 g          478 t
ORIGIN      
        1 ccgttttttt cggtcagccg cacgccgcgg ccgcactggt attcaaaggc attcagaccg
       61 taagagctga gatagtccgg cacctgaaac gccgtcctgt agcccagctc ctcaaaatcg
      121 tcggcgatgc ccgccgtgcc gaaatgcagt tcactcatac aatctaccgc cttttttgta
      181 atagcgggcg aggaaaggcc tctccagcac ccgcttgagc cggatgttcc acttggtgtc
      241 cggcgccacc ggctgcacca gcagcatcgt caccccgtag agcttggctg ccagcgtgtc
      301 ggtgaagatc tggtcgccca ccagcgccat gcgccccttt tccaccccaa aggcccggcg
      361 cgccagccgc agcccgcgcg aggccggctt gaggctgaaa gagacgaacg gcagaccgac
      421 cttttcggca aacgggcgca cccgcctggc gacattattg gagctgatgc gcatctctat
      481 accgctgtgc cgcatcgtgt ccagccagcg ggccacgccg ggcgccagct cttggctgtc
      541 gtgggccgtg agggtgttgt ccacgtccag caccagggcg gtgatgcccc gccgacgcaa
      601 aaattcaggg gtgatgcggt ccacgctttt gaagatgtag tccggacaaa aaacactcaa
      661 ctgggctgca ctcctttttt gctgggacgc cgggcgcact gagaacccgg caaggttcct
      721 gaaagtggca aaaaagcgga cctccggggc aagttctccc tcaggtccgc ttttagttgc
      781 tgtcacagtc gcggcagcct gcaaacttat ttgaacttgc gcttgcgggc ggcttcggac
      841 tttttcttac gtttcacgga aggcttttca tagcactcgc gcttgcgtac ttcggcaaga
      901 acacccgaac gcgcgcagga acgcttaaaa cggcgcagtg cactgtcaag tgattcattg
      961 tccttcactc ggatttccga catctttgtc cctcccttaa cacttgaggt aggagtatga
     1021 cgcccgggag gaagctgcgg agcagttcat ccggccgctc ccgccagaga atcaacatta
     1081 gaattatact ctatttacag cgtccatgtc aacctcaaag ttgagcctga cggcggaaac
     1141 ccgccccggg cctgtaaatt ttttctttgc cgctcagcgg accaccaggt tgaccagctt
     1201 gccgggcaca tagatctcct tgaccaccgt cttgccctcc agcagggcgg ccacgccctc
     1261 cgcctgcttt gcagcggcga tcgcctgtgc ggcgctgacg tccgccgcgg ccacaaagtg
     1321 gccccgcacc ttgccgcaaa cctgcacggc gagctcgatc tgggcgtctt tgcacttgtc
     1381 ctcgtcgtaa acgggccagg cggcgtgggc gatttgcccc tcaaaaccgg ccttctgcca
     1441 caactcctcg gtgatgtggg gggcaaacgg attgagcagc agcaggaaag tgcggtattc
     1501 gtcccgcgtg acgccgcccg cgtcgtccac ctcgttgagc aggctcatca gggtggcgat
     1561 ggcggtgttc atcttgagct gcaggatatc ctcgcccacc tttttgatgg ccttgtggaa
     1621 gctggtctcc agctgcgggc ggtagccctc gccgggcagc agccgctcgg acagatcaaa
     1681 gacccgctcc aaaaagcgct tgcagccctt gatgctggag gaggaccagg gggcggcctt
     1741 ttcaaaatcg ccgatgaaca tctcgtacag gcgcatggtg tcggcgccgt actgatccac
     1801 gatatcatcc gggttgacca cgttgccgcg ggacttggac atcttttcgc cgttctcgcc
     1861 caggatcatg ccgtggctgg tgcgcttggc atacggctcg gcggtgggca ccacgccgat
     1921 gtcgtacaaa aatttgtgcc agaaacgaga gtacagcagg tgcagggtgg tgtgctccat
     1981 gccgccgttg taccaatcca ccggcatcca gtagttgagg gcctcggggc tggcgatggc
     2041 ctggtcgttg tggggatcgc aatagcgcag gaaataccag ctggagcccg cccactgggg
     2101 catggtgtcg gtctcacgcc gcgccgggcc gccgcagcag gggcaggtgg tgttgaccca
     2161 atcggtgaga tgggccagcg ggctctcgcc gtcctcggtg ggctcgtagt tctgcacctc
     2221 cggcagcttc aggggcagct cgctctcagg gaggggctgc cagccgcatt tttcgcagta
     2281 gaccatgggg atgggctcgc cccaatagcg ctggcggctg aacacccagt cgcgcagctt
     2341 gaagctggtt
//
LOCUS       DBMDYA010000480         2350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9613_length_2350_cov_1.677190, whole genome shotgun sequence.
ACCESSION   DBMDYA010000480 DBMDYA010000000
VERSION     DBMDYA010000480.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_2350_cov_1.677190"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(253..>2350)
                     /locus_tag="ACZPFK_10855"
     CDS             complement(253..>2350)
                     /locus_tag="ACZPFK_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1406855.1"
                     /translation="KYGQDLVEAARAHKLDPVIGRDTEIRNVVRILSRKTKNNPVLIG
                     EPGVGKTAIAEGLAQRIVAGDVPEGLKNKTVFSLDMGALVAGAKYRGEFEERLKAVLA
                     EVKKSEGRIILFIDELHTIVGAGKTEGSMDAGNLLKPLLARGELHCIGATTLNEYRQY
                     IEKDAALERRFQPVLVDEPTVEDTISILRGLKERYEVYHGVKITDGALIAAATLSNRY
                     ITDRFLPDKAIDLVDEACATIRTEMDSMPAEMDDVRRQILQHEIEEAALKKEDDKLSQ
                     EHLEEIRRELAGLRDEFAGMKAKWENEKQSIGKVQKLREEIEQVNADIEKAQREYDLN
                     RAAELKYGKLPQLQKELEASEREAVSAGQNQLLRDRVTDEEIAEIVARWTGIPVAKLM
                     EGERQKLLHLDEVLHERVIGQNEAVTKVAEAILRNRAGIGDENRPIGSFLFLGPTGVG
                     KTELAKALAQALFDDEKNMVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRR
                     KPYSVVLFDEIEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTILILTSNLGSPYIL
                     DGIQPDGTISAEARGQVDALLKQSFRPEFLNRLDEIVYYKPLTKGEIGGIVDLLLADL
                     AKRLEAKQLKLSVTAAAKQFIIDGGYDPVYGARPLKRFIQSKVETAIARAILQADPAP
                     GTTLAVDVQDGALTVR"
BASE COUNT          318 a          806 c          740 g          486 t
ORIGIN      
        1 cgccccacgg cctgcacggc aaaacgtcca aagcggcggc atccgcgggc ggcgtgcgcc
       61 gacgccgtca aggcggcggg ccgcaagccg ccggtggggg aaaagcctcg tctctacagc
      121 gcgcaaaaga acggccgcgg aacactgagg tgttccgcgg ccgttctttt ataatggagc
      181 ttcttcaggg cgagcccttg cacaggcgct ctcttttatc ccgtcccgcg tctatgctga
      241 gcggccggta tttcaccgca ccgtcagggc gccgtcctgc acgtccacgg ccagcgtggt
      301 gcccggggcg gggtcggcct gcaaaatggc ccgggcgatg gccgtctcca ccttgctctg
      361 gatgaaccgc ttcagcgggc gcgcgccgta gaccgggtcg tagccgccgt cgatgatgaa
      421 ctgctttgcc gccgcggtga cgctcagctt cagctgcttt gcctccagcc gctttgccag
      481 gtcggccagc agcaggtcca caatgccgcc gatctcgccc ttggtgaggg gcttgtagta
      541 gacgatttcg tccagccggt tgaggaactc gggccggaag ctctgcttca gcagcgcgtc
      601 cacctgcccc cgcgcctcgg cgctgatcgt gccgtccggc tggatgccgt ccaggatata
      661 cgggctgccc aggttggagg tgaggatgag gatggtgttt ttaaaatcca ccgtgcggcc
      721 ctggctgtcg gtgatgcggc cgtcgtccag cacctgcagc agcacgttga aaacgtccgg
      781 gtgcgccttt tcgatctcgt cgaacagcac cacgctgtag ggcttgcggc gcacggcctc
      841 ggtgagctgg ccgccctcct catagcccac atagcccgga ggcgcgccga tcaggcggga
      901 gaccgagaac ttctccatat actcgctcat atcgatgcgc accatgtttt tctcgtcgtc
      961 gaacagggcc tgggccagcg ctttcgccag ctcggttttg cccacgccgg tggggccgag
     1021 gaacaaaaag ctgccgatgg gccggttctc gtcgccgatg cccgcccggt tgcgcaggat
     1081 ggcctcggcc accttggtca ccgcctcgtt ctggccgatg actctctcgt gcagcacctc
     1141 gtccaggtgc agcagcttct gccgctcgcc ctccatcagc ttcgccaccg ggatgccggt
     1201 ccagcgggcc acgatctcgg cgatctcctc gtcggtgacc cggtcgcgca gcagctggtt
     1261 ctgccccgcc gagaccgcct cccgctcgct ggcctccagc tccttttgca gctggggcag
     1321 cttgccgtat ttcagctcgg cggcccggtt caggtcgtac tcgcgctggg ccttttcgat
     1381 gtcggcgttc acctgctcga tctcctcgcg cagcttttgc accttgccga tcgactgctt
     1441 ctcgttctcc catttcgctt tcatgccggc gaactcgtcc cgcagcccgg ccagctcccg
     1501 gcgaatttcc tcaagatgct cctgcgagag cttgtcgtcc tcttttttga gggccgcctc
     1561 ctcgatctcg tgctgcaaaa tctggcggcg cacgtcgtcc atctcggcgg gcatcgagtc
     1621 catctcggtg cggatggtgg cgcaggcctc gtccaccagg tcgatggcct tgtcgggcag
     1681 aaagcggtcg gtgatgtagc ggttgctcag ggtggccgcc gcgatcaggg cgccgtcggt
     1741 gatcttgacg ccgtggtaga cctcgtaccg ctctttcagg ccgcgcagga tgctgatggt
     1801 gtcctctaca gtgggttcgt ccaccagcac cggctggaag cgccgctcca gcgccgcgtc
     1861 cttttcgatg tactgccggt actcgttgag ggtggtggcg ccgatgcagt gcagctcgcc
     1921 ccgggccagc aggggcttta acaggttgcc cgcgtccatg ctgccctcgg tcttgcccgc
     1981 gcccacgatg gtgtgcagct cgtcgatgaa caggatgatg cggccctcgc tctttttcac
     2041 ctcggccagc acggctttca gccgctcctc aaactcgccc cggtactttg cgcccgccac
     2101 cagcgcgccc atgtccaggc tgaaaaccgt cttgttcttc agcccctcgg gcacgtcgcc
     2161 ggccacgatg cgctgggcca ggccctcggc gatggcggtc ttgcccacgc ccggctcgcc
     2221 gatgagcacg gggttgtttt tggtcttgcg ggagaggatg cgcaccacgt tgcggatctc
     2281 ggtgtcgcgg ccgatgaccg ggtccagctt gtgggcccgg gcggcctcca ccaggtcctg
     2341 gccgtacttt
//
LOCUS       DBMDYA010000481         2350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9614_length_2350_cov_1.525122, whole genome shotgun sequence.
ACCESSION   DBMDYA010000481 DBMDYA010000000
VERSION     DBMDYA010000481.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9614_length_2350_cov_1.525122"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            477..962
                     /gene="greA"
                     /locus_tag="ACZPFK_10860"
     CDS             477..962
                     /gene="greA"
                     /locus_tag="ACZPFK_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1406856.1"
                     /translation="MVNNKEMVVTAEGLKALEDELEELKTVKRKEVAEKIKVARGFGD
                     LSENSEYDEAKNEQGLVESRIIFLEKMLKNVRVLDHSELTNDQVMVGSRVKVKDDDGD
                     VDEYSIVGSTEADPMEGKISDESAVGKGLLGKRVGEVAEITVPSGAVVHYEVLEISTA
                     Q"
     gene            1075..>2350
                     /gene="lysS"
                     /locus_tag="ACZPFK_10865"
     CDS             1075..>2350
                     /gene="lysS"
                     /locus_tag="ACZPFK_10865"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1406857.1"
                     /translation="MEENGHAQQAADLLEKAQGAVQEAAAEVKAGARKAAKKAAEVGE
                     ELSAAAYSELLQIRRDKLSALQEAGCDPFRQTKYPVDAHAAGIKADFADTPEGETGRT
                     VSVAGRLMSKRIMGKASFAELRDGSGDIQIYLRRDVVGEEPYAAYKKYDIGDVVGVTG
                     EVFRTKMGEISVKVHEITLLAKSLLPLPEKFHGLKDREARYRQRYVDLIVNPEVKDTF
                     VKRSLILRELRSYLDGKGFLEVDTPILTPFEIGASARPFVTHHNTLDMDMVLRIETEL
                     YLKRLIVGGMERVYEVGRIFRNEGMDPKHNPEFTTIELYQAFTDYFGMMDLVEEMMKT
                     VAQNVCGGLVVPYQGQQIDLSHWERMTMVEAVKKYAGVDFAAVAGDEEAVALAKEHHV
                     ELPEVPTKGAILAEFFDAFVEDKLIQPTFIYDY"
BASE COUNT          514 a          675 c          787 g          374 t
ORIGIN      
        1 cgacaagctg ccccaagcgg atattaccct cgccgacaag gcaaacagcg gcattgtgaa
       61 cgacattttt tatcccctgt tcgttcacgc aaaaaagttt atggaccgct cctccgacga
      121 cgactgaggc aaagcctccc caattttgct ttcccctctt ctcatactcc tctcttaaca
      181 gccgcagggc ccctctttca ggaggggccc tgcggctgtg cgggggggta taagggggcg
      241 gggcggcgca tatactgaga tgtacccatc aggcggcggg cgccgcgcgc ctggaaaaac
      301 atacctgcgg gcagcaagct gtaaaattgt gaaaagcgcc tcgcgcactt gacacggggc
      361 ggcttctata ctataataca acttactgtt tagccggggt ggggcatacc ctctccccgg
      421 tctttcgcat attatgcggg cggtggagcg aaccccgccg aggaaaagga gacaggatcg
      481 tgaacaacaa agaaatggtc gtcaccgccg agggcctgaa agccctggag gacgagctgg
      541 aagagctgaa aacagtcaaa cgcaaagagg tcgcggagaa gatcaaggtc gcgcgcggct
      601 ttggcgacct gtctgaaaac agcgaatacg acgaggccaa aaacgagcag ggcctggtgg
      661 agagccgcat catctttttg gagaagatgc tcaaaaacgt gcgcgtgctg gaccacagcg
      721 agctgaccaa cgaccaggtc atggtcggca gccgcgtcaa ggtcaaggac gacgacgggg
      781 atgtggacga gtacagcatc gtcggctcca ctgaggcgga cccgatggag ggcaagatct
      841 cggacgagag cgcggtgggc aagggcctgc tgggcaagcg cgtgggcgag gtggccgaga
      901 tcacagtgcc cagcggcgcg gtcgtccact acgaggtgct ggagatctcc accgcgcagt
      961 gaccgcccaa agacagcaga tagagggggg cggcactgcc gcccctcttt ttggttcgag
     1021 cagggggcag cccggcggca gtccgggcgc agggaagtaa aaaggaggcg caatatggaa
     1081 gaaaatggac atgcgcagca ggccgcggac ctgctggaaa aggcgcaggg cgcggtgcag
     1141 gaggcggccg ccgaggtgaa agccggggcc cgcaaggccg caaaaaaagc cgccgaggtg
     1201 ggggaggagc tctccgccgc ggcctacagc gagctgctgc agatccgcag ggacaagctg
     1261 tcggcgctgc aggaggcggg ctgcgacccg ttccggcaga ccaaataccc ggtggacgcc
     1321 cacgcggccg ggatcaaggc ggactttgcc gacacgccgg agggcgagac cggcaggacc
     1381 gtgtcggtgg cgggccgcct gatgagcaag cgcatcatgg gcaaggccag ctttgccgag
     1441 ctgcgggacg gctctggcga catccagatc tacctgcgcc gcgacgtggt gggcgaggag
     1501 ccctacgcgg cctataaaaa atatgacatc ggcgacgtgg tgggggtgac gggcgaggtt
     1561 ttccgcacca agatgggcga gatctcggtc aaggtgcacg agatcaccct gctggcgaaa
     1621 tccctgctgc cgctgcccga aaaatttcac ggtctaaagg accgggaggc ccgctaccgt
     1681 cagcggtatg tggacctgat cgtcaacccg gaggtcaagg acacctttgt caagcgcagc
     1741 ctcatcctgc gcgagctgcg cagctatctg gacggcaagg gctttctgga ggtggacacc
     1801 cctatcctca cgccgtttga gatcggcgcc tcggcgcggc ctttcgtcac ccaccacaac
     1861 acgctggaca tggacatggt gctgcgcatc gagaccgagc tgtacttaaa gcggctgatc
     1921 gtgggcggca tggagcgggt ctacgaggtg gggcgcatct tccgcaacga gggcatggac
     1981 cccaagcaca accccgagtt caccaccatc gagctctacc aggcgttcac cgattatttc
     2041 ggcatgatgg acctggtgga ggagatgatg aagaccgtgg cccaaaacgt ctgcggcggc
     2101 ctggtggtgc cctaccaggg ccagcagatc gacctctccc actgggagcg gatgaccatg
     2161 gtggaggcag tgaaaaaata tgcgggcgtg gattttgccg ccgtcgccgg cgacgaggag
     2221 gcggtggccc tggccaagga gcaccatgtg gagctgcccg aggtgcccac caagggggcg
     2281 atcctggccg agttcttcga cgcctttgtg gaggacaagc tcatccagcc caccttcatc
     2341 tatgattacc
//
LOCUS       DBMDYA010000482         2342 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9644_length_2342_cov_1.342704, whole genome shotgun sequence.
ACCESSION   DBMDYA010000482 DBMDYA010000000
VERSION     DBMDYA010000482.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9644_length_2342_cov_1.342704"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="ACZPFK_10870"
     CDS             complement(<1..661)
                     /locus_tag="ACZPFK_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538204.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1406858.1"
                     /translation="MVYRIYVEKRPGFDVEAQGLLEELRGLLGIRGLAGLRLLNRYDV
                     EGISDELFRQCVGTVFSEPQLDLTYGELPPQQGEGAVFAVEYLPGQFDQRADSASECI
                     QLISCSERPMVRTAKVYLLSGSLSAQELAAVKKYVINPVEAREASLEARQTLAASYPE
                     PGKVEVLAGFNDLDKAGLAAFVQKYGLAMDEDDAAFCQQYFRSEQREPTITEIRMIDT
                     YW"
     gene            complement(692..1978)
                     /gene="purD"
                     /locus_tag="ACZPFK_10875"
     CDS             complement(692..1978)
                     /gene="purD"
                     /locus_tag="ACZPFK_10875"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1406859.1"
                     /translation="MKVAVIGGGGREHAIIRKLKENPQISALYALPGNGGIGYDAVCV
                     DIRATDLEGIVAFAVREALDFVVVAPDDPLALGLVDRLEAAGIPAFGPTAKAAQIESS
                     KVFSKGLMKQYGIPTARYEVFADPGAARAYLEANTRWPIVLKADGLALGKGVLICKDR
                     AAALDGVRTIMEDRVFGAAGSQMVVEEFLTGPEVSVLAFTDGKAMVPMVSSMDHKRAL
                     DGDEGLNTGGMGTVAPNPYYTGAVAKECMEKIFLPTMAAMNAEGRTFKGCLYFGLMLT
                     PDGPKVIEYNCRFGDPETQVVLPLLESDLFTVMRAVSAGRLSETPVRFRDGAAACVVL
                     ASGGYPQKYATGLPIEGLEEGQLPGAEGVTVYHAGTRRQDGGLVTAGGRVLGVTAAAS
                     DLPAALKRAYAAAERIRFEGMHYRRDIGKRALEARG"
     gene            complement(2065..>2342)
                     /locus_tag="ACZPFK_10880"
     CDS             complement(2065..>2342)
                     /locus_tag="ACZPFK_10880"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922295.1"
                     /note="Catalyzes the formylation of AICAR with
                     10-formyl-tetrahydrofolate to yield FAICAR and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1406860.1"
                     /translation="VLGDEVWRNTFAVRPEPLTAAEKKSYLAGIKGVVLGSDAFFPFG
                     DNIERAHRSGVEAIVEPGGSIRDDQVIETCDKYGIVMAFCGIRLFHH"
BASE COUNT          382 a          847 c          702 g          411 t
ORIGIN      
        1 tccagtaggt gtcgatcatg cggatctcgg tgatggtggg ctcgcgctgc tcagagcgga
       61 agtactgctg gcagaacgcg gcgtcatcct catccatggc gagaccatat ttttgcacaa
      121 aggccgccag accggccttg tccaggtcgt tgaagccggc caggacctcc accttgccgg
      181 gctcgggata ggaggcggcc agcgtctgac gcgcctccaa tgaggcctcc cgcgcctcca
      241 ccggattgat gacgtatttt ttgacggccg ccagctcctg cgccgagagg ctgcccgaga
      301 gcaggtagac ctttgcggtg cgcaccatgg gccgctcgga acagctgatc agctggatgc
      361 actcggaggc tgaatccgcc cgctggtcga actggccggg cagatattcc accgcaaaaa
      421 ccgcgccctc cccctgctgc gggggcagct cgccgtaggt caggtccagc tgcggctcgc
      481 tgaagaccgt tcccacgcac tggcggaaca gctcgtcaga gatgccctcc acgtcgtagc
      541 ggttcagcag ccgcagcccg gccagccctc ggatgcccag caggccgcgc agctcctcca
      601 gcaggccctg cgcctccacg tcaaaaccgg ggcgtttttc cacataaatg cgataaacca
      661 tgcgctgtgt gctcctttat cactttactc gttatccgcg cgcctccagc gcccgtttgc
      721 cgatatccct gcggtagtgc atgccctcaa agcggatccg ctccgccgcg gcataggccc
      781 gtttcagggc cgccggcagg tcggacgctg cggcggtgac ccccagcacc cgacccccgg
      841 ccgtgaccag cccgccgtcc tgccgccgcg tgcccgcgtg gtagacggtg accccctccg
      901 caccgggcaa ttgcccctct tccaggccct cgatgggcag gccggtggca tacttctgcg
      961 gatagccgcc cgaggccagc accacacagg cagccgcccc gtcccggaac cgcaccggcg
     1021 tctccgaaag ccggcccgcg ctcacggccc gcatcaccgt gaacaggtcg ctctccagca
     1081 gcggcagcac cacctgggtc tccgggtcgc cgaaccggca gttatactcg atgaccttgg
     1141 ggccatccgg ggtgagcatg aggccgaaat agagacagcc cttgaacgtg cgcccctcgg
     1201 cgttcatggc cgccatggtg ggcagaaaga tcttctccat acactctttg gccacggccc
     1261 ctgtataata ggggtttggg gccaccgtgc ccatgccgcc ggtgttcagg ccctcgtctc
     1321 cgtccagcgc ccgcttgtgg tccatactgc tgaccatggg gaccatggcc ttgccgtcgg
     1381 taaaggccag cacgctgacc tccggcccgg tgaggaactc ctcgaccacc atttggctgc
     1441 ccgcggcccc gaaaacccgg tcctccatga tggtgcgcac cccgtccagg gcggcggccc
     1501 ggtccttgca gatcagcacg cccttgccca gcgccaggcc gtccgctttc agcacgatgg
     1561 gccagcgcgt gttcgcctcc aggtacgccc gggccgcgcc ggggtcggca aagacctcat
     1621 agcgggcggt ggggatgccg tattgtttca tcagcccttt gctgaacacc ttgctgctct
     1681 cgatctgggc cgctttggcg gtggggccga aagcggggat gcccgcggcc tccagccggt
     1741 ccaccagccc cagggccagc gggtcgtccg gcgcaaccac cacaaaatcc agcgcctccc
     1801 gcactgcaaa ggccacaata ccctccaggt cggtggcgcg gatatcaacg cagacggcgt
     1861 catacccgat ccccccgttg ccgggcagcg cgtagagcgc gctgatctgt gggttctctt
     1921 tcagcttgcg gatgatggcg tgctcacgcc cgccgccgcc gatcactgct actttcattg
     1981 ccgcttcctc cctctctcaa caacaagaaa agcccagagc ccggccccgg gccggtcgtg
     2041 agcttttctt gcgccctctg cgcatcagtg atggaacagc cggatgccgc agaacgccat
     2101 gacgatgccg tatttgtcgc aggtctcgat gacctggtcg tcgcggatcg agccgcccgg
     2161 ctccacgatg gcctccacgc cgctgcggtg ggcgcgctcg atgttgtccc caaacgggaa
     2221 aaaggcgtcg ctgcccagca ccacgccctt gatgccggca agatagctct ttttctccgc
     2281 cgctgtcagc ggctcgggcc gcacggcaaa ggtgttgcgc cagacctcgt ctcccagcac
     2341 at
//
LOCUS       DBMDYA010000483         2341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9653_length_2341_cov_1.188839, whole genome shotgun sequence.
ACCESSION   DBMDYA010000483 DBMDYA010000000
VERSION     DBMDYA010000483.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_2341_cov_1.188839"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="recO"
                     /locus_tag="ACZPFK_10885"
     CDS             complement(<1..640)
                     /gene="recO"
                     /locus_tag="ACZPFK_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1406861.1"
                     /translation="MAQQVKTTGIVLREVRTGESDRILTILTPGHGVISASAKSSQRL
                     KNKLFAATGLFCYSEFVLFEGKTMYIVDEATPLEVFFDLRGSVEGMSLAMYLAELMGT
                     LAPEGEEAGAQLRLLLNSLALICRRRNLYQVKAVCELRALSHAGYMPDLVACAGCGCY
                     EGDAFFFDVAQGRLLCAECAAKAGRAVNLPGAELSAMRHILYSGDDKVFSFIL"
     gene            complement(585..755)
                     /locus_tag="ACZPFK_10890"
     CDS             complement(585..755)
                     /locus_tag="ACZPFK_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406862.1"
                     /translation="MTEQDYWQRFAASGDIADYILYRGSSIAAEMRAKESAGYGTAGE
                     DHGDRPARSQDG"
     gene            complement(918..>1146)
                     /locus_tag="ACZPFK_10895"
     CDS             complement(918..>1146)
                     /locus_tag="ACZPFK_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1406863.1"
                     /translation="DGGLVDIEVTIFCEKKSHKGMVIGRGGAMLKKIASAARADCEEF
                     LGQRVNLQCWVKVKDDWRDNDFYLNNFGFRQ"
     assembly_gap    1147..1156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1157..1827)
                     /gene="era"
                     /locus_tag="ACZPFK_10900"
     CDS             complement(<1157..1827)
                     /gene="era"
                     /locus_tag="ACZPFK_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1406864.1"
                     /translation="MTSSTGTKSLFVAIVGRPNVGKSSLLNALIGEKLAIVTSKPQTT
                     RTRITGILTRGRTQFVFLDTPGIHQPRTKLGKRMVKTVEDTVGDGDVTLMVFEPYGDL
                     TEAEMGLVQEIRTSRTPAVAVVNKTDCLKKAADLNVRRQFLEGLGVFREIVGASAVER
                     QGLDALLDLLEGYAAPGPHYFPDDAYTDLPEKAIVAELVREKLLLNLRDELPHGTAVS
                     VERFH"
     gene            complement(1805..>2341)
                     /locus_tag="ACZPFK_10905"
     CDS             complement(1805..>2341)
                     /locus_tag="ACZPFK_10905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR1406865.1"
                     /translation="APHHRLHAGIKGGGHPLRKSGFFRGFGYAWSGLVTAVREERNLR
                     FHLVAGAFVLYFSRFYDFTRGERLLLVALICGVVALELVNSSIERAVARPGPDKFFIA
                     GVVKDMAAAAVLVFSIGAAVCGAVLFFDPAALRSMLNHFLSAWYRLPLLLAAATAGYL
                     FVFHYRKGARSFDLFDRD"
BASE COUNT          458 a          796 c          675 g          402 t
ORIGIN      
        1 gcaagataaa gctgaacacc ttgtcatcgc cgctgtacag gatgtggcgc atggccgaga
       61 gctcggcccc cggcaggttc acggccctgc cggcctttgc cgcgcactcg gcgcagagca
      121 gccgcccctg tgccacgtca aagaaaaagg catccccctc gtagcagccg cagcccgcgc
      181 aggccaccag gtcgggcatg tagcccgcat gcgagagcgc gcgcagctcg cacaccgcct
      241 tgacctgata caggttgcgg cggcggcaga tgagggcgag gctgttcagc agcagccgca
      301 gctgggcccc ggcctcctcg ccctccgggg caagggtgcc catcagctcg gccagataca
      361 tggccaggct catcccctcc acgctgccgc gcaggtcgaa aaacacctcc agcggggtcg
      421 cctcgtccac gatatacatg gtcttgccct caaagagtac gaactcggaa taacagaaaa
      481 gccccgtcgc ggcaaagagc ttgttcttga gccgctgaga gcttttggcc gacgccgaaa
      541 tcaccccgtg gccgggggtg aggatggtaa gtatccggtc ggactcaccc gtcctgactt
      601 ctcgcaggac gatccccgtg gtcttcacct gctgtgccat aaccggcgct ctcctttgcc
      661 cgcatttcgg ccgcgatcga gctgccgcgg taaaggatat aatccgcgat gtccccgctc
      721 gccgcaaacc tctgccaata atcctgttcc gtcatgcctt gccgcctccc ggtaccagtt
      781 tctatcctta ctgttaccgg gagaaaagcc ccccatacac ggaaaagtgt ggcaggcgca
      841 aagcgcctgc cacagcgttc tctcccctgt tttatcctat cacaggcggg gcgcccgcgt
      901 caatgcggcc gcgcgcccta ttgacgaaag ccgaagttgt tcagataaaa atcgttgtcc
      961 cgccagtcgt ccttgacctt gacccagcac tgcaggttca cccgttggcc gagaaactcc
     1021 tcgcagtcgg cccgcgccgc gctggcgatc tttttgagca tcgccccgcc cctgccgatc
     1081 accatgccct tgtggctctt tttttcgcaa aaaatggtga cctcgatatc caccaggccg
     1141 ccgtccnnnn nnnnnntcgt ggaacctttc caccgagacc gccgtgccgt ggggcagctc
     1201 atcgcgcagg ttcagcagca gcttttcgcg caccagctcg gccacgatgg ccttttcggg
     1261 cagatcggta taggcgtcgt cgggaaaata atgcgggccc ggggcggcgt atccctccag
     1321 caggtccaaa agagcgtcaa gcccctgccg ttccaccgcc gaagccccca cgatctcccg
     1381 aaacacgccc agaccctcca gaaattggcg ccgcacattc aggtcggcag cctttttcag
     1441 gcagtcggtc ttgttgacga ccgccaccgc cggggtgcgg gaagtgcgga tctcctgcac
     1501 cagccccatc tccgcctcgg tcaagtctcc atagggctca aagaccatca gcgtcacgtc
     1561 gccgtcgccc acggtgtcct ccaccgtctt caccatccgc ttgcccagct tggtgcgggg
     1621 ctggtggatg cccggcgtat ccagaaacac gaactgggtc cgcccgcggg tgaggatgcc
     1681 ggtgatgcgg gtgcgggtgg tctggggctt tgaggtgacg atggccagtt tttccccgat
     1741 cagggcgttg agcaggctgg acttgcccac gttgggcctg cccacaatgg cgacaaaaag
     1801 ggatttagtc ccggtcgaag aggtcaaagc tgcgcgctcc ttttctgtaa tggaatacga
     1861 agagataccc agccgtcgcc gcggccaaca gcagcggcag acggtaccag gcggagagga
     1921 aatggttcag catcgagcgc agtgccgccg gatcaaaaaa gagcacggcg ccgcacacgg
     1981 ccgccccgat gctgaagacc agcaccgcgg ccgccgccat atccttgacc accccagcga
     2041 taaagaactt gtccggcccg ggccgggcga ccgcccgctc gatggacgag ttgaccagct
     2101 ccagcgccac gacgccgcag atcagcgcca ccagcagcag ccgctcgccc cgcgtgaaat
     2161 cataaaagcg ggaaaaatag agcacaaacg cacccgccac cagatgaaag cgcagattgc
     2221 gctcttcccg cacggcggtg acaaggccgg accaggcgta gccgaagccg cgaaaaaaac
     2281 cgctctttcg caagggatgc ccgcccccct ttattccggc gtgtaggcga tggtgcgggg
     2341 c
//
LOCUS       DBMDYA010000484         2326 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9734_length_2326_cov_1.435056, whole genome shotgun sequence.
ACCESSION   DBMDYA010000484 DBMDYA010000000
VERSION     DBMDYA010000484.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9734_length_2326_cov_1.435056"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /gene="feoB"
                     /locus_tag="ACZPFK_10910"
     CDS             complement(<1..1398)
                     /gene="feoB"
                     /locus_tag="ACZPFK_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HIR1406866.1"
                     /translation="MGLTSGSTGIHAVDTGLVVKKQRESDLVVALAGNPNVGKSTVFN
                     ALTGMNQHTGNWPGKTVANAQGGCTHKGQGFVLVDIPGSYSLMAHSAEEEVARDFICF
                     GAPDAVAVVCDATCLERNLNLVLQTLEITGSVVVCVNLMDEARRKGVSLDLGLVSERL
                     GVPVIPAAARSGEGLKELMDALAALPRESRAKTHTIRYPKPVEQAAALLEPALEALCG
                     DRLPARWAALRLLDGDEKLRRSLSEFCGVGLEEQPAVAAALARARALLTQAGLTGEKL
                     EDAIVSALVREAERTCRGAVRVQPTRQELRDRKIDRALTSKWFGYPLMLLLLAAVLWL
                     TIAGANVPSELLSKGLFWVQDQLSRAFLALHAPEWLRGALVDGVFRTLAWVVSVMLPP
                     MAIFFPLFTLLEDLGYLPRVAFNLDKYFKKCCACGKQALTMCMGFGCNAAGIVGCRII
                     DSPRERLIAMITNNFV"
     gene            complement(1439..1675)
                     /locus_tag="ACZPFK_10915"
     CDS             complement(1439..1675)
                     /locus_tag="ACZPFK_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1406867.1"
                     /translation="MTGIDSLTNLKVGQRARVTALHSGGSMRRRLQDIGLIAGTEVEC
                     VQKSPAGDPVAYLIRGALIALRSEDSAGVMIACP"
     gene            1778..2209
                     /locus_tag="ACZPFK_10920"
     CDS             1778..2209
                     /locus_tag="ACZPFK_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1406868.1"
                     /translation="MDGPSHFHTQKGYELAGGSPLTAAMEDYLEMLCRLCSTEGFTRV
                     HLLAERLNVQPSSASKMVDGLKREGLVRAEKYGLLRLTPRGEEVGRYLLYRHGVLHRA
                     LCLLNHTDSELEQTERIEHFIDRRTVENLARLLEQHDAPPR"
BASE COUNT          407 a          833 c          710 g          376 t
ORIGIN      
        1 cacaaagttg ttggtgatca tggcgatcag ccgctcccgc ggcgagtcga tgatacggca
       61 gccgacgatg cccgccgcgt tgcagccgaa gcccatgcac atggtgaggg cctgcttgcc
      121 gcaggcgcag cactttttaa aatacttatc caggttgaaa gccacccggg gcagatagcc
      181 cagatcttcc agcagggtaa acagcggaaa gaaaatcgcc atgggcggca gcatcaccga
      241 gaccacccag gccaaggtgc ggaagacgcc gtccaccagc gcgccccgca gccattccgg
      301 cgcatggagg gcgagaaacg cgcggctgag ctgatcctgc acccagaaca ggcctttcga
      361 cagcagctca gagggcacat tggcgcccgc gatggtcagc cagagcaccg ccgccagcaa
      421 cagcagcatc agcggatagc cgaaccactt gctggtgagg gcccggtcga tcttgcggtc
      481 ccgcagctct tgtcgggtgg gctgcacccg cacggcgccg cggcaggtgc gctcggcctc
      541 ccgcaccagc gccgagacaa tcgcgtcctc cagcttctcc cctgtgaggc cggcctgtgt
      601 caacagcgcc cgcgcccggg ccagggcggc cgccacggcc ggctgctcct ccaaccccac
      661 gccgcagaac tccgacaggg agcggcgcag tttttcatcc ccgtccagca gccgcagcgc
      721 cgcccagcgg gcgggcagcc tgtcgccgca gagcgcttcc agcgccggtt ccagcagggc
      781 cgccgcctgc tccaccggct ttgggtagcg gatggtgtgg gtcttggccc ggctctcccg
      841 cggcagggcc gccagggcgt ccatcagctc tttcagcccc tcgccgctgc gggccgccgc
      901 cgggatcacc ggcaccccca gccgctcgga taccagcccc aggtccagcg agacgccctt
      961 gcgccgcgct tcgtccatca ggttgacgca caccaccacg ctgccggtga tctccagcgt
     1021 ctgcagcacc aggttgaggt tgcgctccag acaggtggcg tcacagacca cggccaccgc
     1081 gtccggcgcg ccaaagcaga tgaaatcccg cgcgacctcc tcctccgccg agtgcgccat
     1141 cagcgaatag ctgcccggga tatccaccag cacgaagccc tgccccttgt gggtgcagcc
     1201 cccctgcgca ttcgccaccg ttttgcccgg ccagttgccg gtatgctggt tcatgcccgt
     1261 cagggcgttg aacaccgtgg atttgcccac gttggggttg cccgccaggg ccaccaccag
     1321 atcagattcc cgctgctttt tcacgaccag gcccgtgtcc accgcatgga tgccggtgga
     1381 gccgctggtc agacccaagc cccatcacct ctcccgtctc tgttttgtcc gccctctttc
     1441 aggggcaggc gatcatcacc ccagccgaat cctctgagcg cagggcgatc agcgcgccgc
     1501 ggatcaggta ggccaccggg tcgccggccg ggcttttttg cacgcattcc acctctgtgc
     1561 ccgcgatcag gccgatgtcc tgcaggcggc ggcgcatact gccacccgaa tgcagggcgg
     1621 tcacccgcgc ccgctggccc accttgagat tggtcaaact atcaataccg gtcataaccg
     1681 attcctcctc gccatatgtt agggacagga aactttcttt cctgttgcca atatatgcgg
     1741 cccgcacgga tgtgtgccgc gggaggaggc tccggggatg gacgggccgt cgcattttca
     1801 cacccaaaag ggctacgagc tcgccggggg cagcccgctc accgccgcca tggaggatta
     1861 tctggagatg ctctgccgcc tgtgcagcac agagggcttt acccgcgtac acctgttggc
     1921 ggagcggctc aacgtccagc cctcctccgc ctccaaaatg gtggatgggc tgaagcggga
     1981 gggcctggtg agggctgaaa aatacggcct gctgcgcctg acgccccgcg gcgaggaggt
     2041 gggccgctac ctgctctacc gtcacggcgt gctgcaccgg gccctctgcc tgctgaacca
     2101 caccgacagt gaactggaac agaccgagcg catcgagcac tttatcgacc gccgcacggt
     2161 ggaaaacctg gcccgtctgc tggaacagca cgacgccccg ccccgctgag gctccgggcg
     2221 gtaaaaactg ttgtaaagcc gccggggcag gcgccccgcc gccgcgcggc aaccgaaacg
     2281 gatggccgaa aaaacgaccg ccgctttgct gcggctattt tttatt
//
LOCUS       DBMDYA010000485         2308 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9803_length_2308_cov_1.333484, whole genome shotgun sequence.
ACCESSION   DBMDYA010000485 DBMDYA010000000
VERSION     DBMDYA010000485.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_2308_cov_1.333484"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="ACZPFK_10925"
     CDS             <1..256
                     /locus_tag="ACZPFK_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013527.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR1406869.1"
                     /translation="GIGKKTAAQLLHQFDGLEPLIARAQEVQKPSLRAAIAQNAGLLR
                     RNYRLIKLEAGAPLPFCREELRYRYNGLTTNEVLAGIGLK"
     gene            complement(413..1063)
                     /locus_tag="ACZPFK_10930"
     CDS             complement(413..1063)
                     /locus_tag="ACZPFK_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013003.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR1406870.1"
                     /translation="MIREPLDFSGAVVFDEQGYLAEFLARAAEDETPVEDLVFHRLTF
                     DNLLLTGLELRRCRFEGCRFVACRLPKLYLRDCALEQCDLSGCRLTDATYWRVFWKDC
                     KFSGTQFASSSLRECSFLRCAGEFVNFSGCSLRQTGFEECRLPEASFDSCKPVRLSFA
                     DCALRRADFSGTALKGIDLSSDDIEGMLAAGPELRGAVVSALQACELARLLGVIIK"
     gene            complement(1060..1680)
                     /locus_tag="ACZPFK_10935"
     CDS             complement(1060..1680)
                     /locus_tag="ACZPFK_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406871.1"
                     /translation="MSKNELKMPLIEDGARWAYGGDQEWCPSAWHRGCGCGPTTGANL
                     SAYYAMTRPSMRGLYRGSLSPFSKQEYIDTIEDIFRFITPSYMGFPYAGKYALKYRAY
                     ALYRGVRLSSRGCGAAHSEDEMFDFVRRGIDSGNPVALLILRHRAPELEDDNWHWVTV
                     SGYLEGADGKQVILSNCGEREVRPASMLFERHPKNTVRLLYFQEEA"
     gene            complement(1827..>2308)
                     /locus_tag="ACZPFK_10940"
     CDS             complement(1827..>2308)
                     /locus_tag="ACZPFK_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406872.1"
                     /translation="GDGLSVAAVSLVGQSMGQRRVDLAQVYGSICQRIGFLISILLGI
                     VFFTCGRGIVGLFTTDPAILDNGSVILDMMSLIVLLQISQVIFNGCLRGAGDTRFVAV
                     ISLISVTFVRPFSSWLLCYPLGWGLYGAWIGLAIDQIVRFALSRYRFGSGKWTKIEI"
BASE COUNT          498 a          701 c          745 g          364 t
ORIGIN      
        1 cgggatcggc aaaaagacgg cggcgcagct gctccaccag tttgacggac tggaacccct
       61 gatcgcccgt gcgcaggagg tgcaaaagcc ctctctccgc gcagcgattg cccaaaacgc
      121 aggcctgctg cgcaggaact acaggctcat aaaactggag gccggggcgc cgctgccgtt
      181 ttgcagagaa gagctgcggt atcggtataa cgggttgaca acgaatgagg tgctggccgg
      241 gatcgggctg aaatagagcg ccggggccgg caggcttttc tgttgtttga aaaaccgacc
      301 tgcaaaagca gctcggtttg cccgccgccc gatttcaaac aaacacaccc ggccgtcact
      361 tcgacggccg ggtgtttgca aaagcgagaa aagagcgtga caggggacag ggctacttga
      421 tgatgacccc cagcaggcgg gccagctcgc aggcctgcag agcactgacg acagcgcccc
      481 gcagctcggg ccccgcggcc agcatgccct cgatgtcgtc gctgctcaga tcgatcccct
      541 tgagggccgt gccgctgaaa tccgcccggc gcagcgcgca gtcggcaaag ctgaggcgca
      601 ccggcttgca gctgtcgaag ctggcctcgg gcaaacggca ctcctcaaag ccggtctggc
      661 gcagggaaca gccgctgaaa ttgacaaatt ccccggcgca gcgcagaaag ctgcactccc
      721 gcagtgagct ggaggcgaat tgtgtgccgg aaaatttgca gtctttccag aaaacgcgcc
      781 aataggtggc gtcggtcagg cggcagccgg acaggtcgca ttgctccagc gcgcagtcgc
      841 gcagatagag cttgggcaga cggcaggcga cgaagcggca accctcaaac cggcatcggc
      901 gaagctcgag gccggtcagc agcagattgt cgaaagtcag gcggtggaag acgaggtcct
      961 ccaccggcgt ctcatcctcc gccgcccgcg cgaggaactc ggccagatag ccctgctcgt
     1021 cgaacaccac cgcgccggaa aagtccagag gttcccgtat catgcttcct cctgaaaata
     1081 gagcaggcgc accgtgtttt tggggtggcg ctcgaacagc atggaggcgg gccgcacctc
     1141 gcgctcgccg cagttgctca gaatgacctg cttgccgtcc gccccctcaa ggtagccgct
     1201 cacggtcacc cagtgccagt tatcgtcctc cagctccggc gcgcggtggc gcagaatcag
     1261 cagggccacg gggttgccgg agtcgatgcc ccggcgcaca aaatcgaaca tctcatcctc
     1321 gctgtgggcg gcgccgcagc ctctgctgga caggcgcacc ccgcggtaaa gggcgtacgc
     1381 ccggtacttg agggcgtatt ttccggcgta ggggaagccc atgtagctgg gggtgataaa
     1441 gcggaaaatg tcctcgatgg tgtcgatgta ctcctgcttg gagaaagggg agagggagcc
     1501 ccggtaaagg ccgcgcatcg aggggcgggt catggcgtag taggcggaaa ggttggcgcc
     1561 cgtcgtgggg ccgcagccgc agccgcggtg ccaggcggag gggcaccatt cctggtcgcc
     1621 gccgtaggcc cagcgcgcgc cgtcctcgat cagaggcatt ttgagttcat ttttggacaa
     1681 agcgggtcac ctcctgaaga aatgcgtttt tcgccgcgcc gcagcccggc gaaaaaagag
     1741 agcagcgccc cccagctgta atcagagcaa aagaggcact gctttttatt atagcacgtt
     1801 tgtccacaga tagcacttaa gagggttcag atttcgatct tggtccattt gccgctgccg
     1861 aagcgatagc gggacagcgc gaagcggacg atctgatcga tggctaagcc gatccaggcg
     1921 ccgtagagac cccagcccag cggatagcag agcagccagc tggaaaaggg gcgcacaaag
     1981 gtgacgctga tgaggctgat gaccgccaca aaccgggtgt cgccggcccc gcgtaggcag
     2041 ccgttgaaaa taacctggga gatctgcagc aacacgatga gggacatcat atccaggatg
     2101 accgagccgt tgtccaaaat ggcggggtcg gtcgtgaaaa gccccacgat ccctcgtcca
     2161 caggtgaaaa agacgatccc cagcaggatc gagatcaaaa agccgatgcg ctggcagatg
     2221 ctgccgtaca cctgcgccag gtccacgcgg cgctgcccca tgctctgccc aaccagggag
     2281 acggcggcca ccgagaggcc gtcgccaa
//
LOCUS       DBMDYA010000486         2302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9836_length_2302_cov_1.700591, whole genome shotgun sequence.
ACCESSION   DBMDYA010000486 DBMDYA010000000
VERSION     DBMDYA010000486.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9836_length_2302_cov_1.700591"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            288..833
                     /locus_tag="ACZPFK_10945"
     CDS             288..833
                     /locus_tag="ACZPFK_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406873.1"
                     /translation="MSENVTAYAPYDSEGKRMPLACPVEYLYDENGYGAGERRFASAE
                     QGAKADALAAGAAYRTEEVKTGETWIDGRPIYRKILKTAVPGNGVFWYALGSSIDMCV
                     KIEGIICDTVNNSVSPLPIINPWLNISDTIRLSANTDRATSNKNSILIASAGNEFKGQ
                     PIYLTVYYTRPTDAPEVTSNG"
     gene            826..2232
                     /locus_tag="ACZPFK_10950"
     CDS             826..2232
                     /locus_tag="ACZPFK_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019236.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HIR1406874.1"
                     /translation="MAEELTTKQFVDIETGKPISNPVYIGDVYDKDGADAVLRLFGQD
                     KTRDTCGRVGDYRITDDPVLAENHLWPDGGPIDPVAWPELHAYAARLGWQTDAAGRFL
                     LPDLRGKYLVGADSRDPDFAAGKESGEKAHTLTKAELPNVSIGLGAAVIKSDTEAIRV
                     TDSGPLWYRMGRSVQETAGPLGNGEPHNNLPPYRAVYIQLRAKPDPVVAQEVAADRLA
                     TARRIGSASFDGSADITLAQMGVITQSNMTLHVSPTGNDNTGKGTQGAPYKTIARAIQ
                     DVPQIVNHTITISLAPGNYGSETISLGMSGKGALQIVGSGNSLANATNYKFGYLRFFN
                     FACSLNIYGISTTSSPDWGGSLQFENCSHVFVQYCDCQAGKDNGKAGVYMDKGYCYVH
                     TCNFHGCSSAVFAVRGGVMAVTNSTGNNNTTGFNASMGGVAVKMVDQTNNLGGTNATS
                     AYNGGIIVGPGGGIIGNI"
BASE COUNT          597 a          579 c          687 g          439 t
ORIGIN      
        1 acagcatcgc taagatactc ggtcaccggg acgttgaggt gacggcccgc atctacgtac
       61 acaacgagct ggacgagctc aaacgggcga cgcgctacac gcgccgtcag cagccgctcc
      121 cgatccagcc ctccgccgcg tagcctctgc cgccgcccgc ccccgatgac tgttgagata
      181 gacatcatca ggaaggataa catgcaaaaa gcgtacatgc ccagagaggc ccagcggtta
      241 tccgggatga atgcggacga agcctaaaat agtggaggcg aaagaaaatg tcagaaaacg
      301 tgacagccta cgcgccgtat gacagcgagg gcaagcggat gccgctggcc tgcccggtgg
      361 aatatctgta cgacgaaaac gggtacggtg ccggggagcg gcggtttgca tcggcggagc
      421 agggggcaaa agcggacgcg ctggccgctg gagcggccta ccgcaccgaa gaggtcaaga
      481 cgggggaaac ctggattgac gggcggccga tttatcggaa aattttaaaa acggcagtgc
      541 cggggaacgg ggtattctgg tatgcgctgg gaagttcaat agatatgtgc gtaaaaatag
      601 aggggataat ttgcgatact gtgaacaact ctgtgtctcc tcttcctatc atcaatcctt
      661 ggcttaacat ctcagacacc atccgtttaa gtgccaacac agatagagcc acatcgaata
      721 aaaacagcat actaattgca tcggccggaa atgaatttaa aggacaaccg atttatctaa
      781 ccgtttacta tactagaccc acggacgcgc cggaggtgac gagcaatggc tgaagaactg
      841 acgacaaaac aattcgtgga catcgagacg ggcaagccga tctcaaaccc ggtgtacatc
      901 ggggatgtgt acgacaagga cggcgcggac gcggtgctgc ggctgttcgg gcaggacaag
      961 acgcgggaca cctgcggacg ggtgggtgat taccgcatca ccgacgaccc ggtgctggcg
     1021 gaaaaccacc tctggccgga cggcggcccc atcgacccgg tggcctggcc ggagctgcac
     1081 gcctacgccg cgcgcctcgg ctggcagacc gatgcggcgg gtcggtttct gctgccggac
     1141 ctgcggggga agtacctggt gggcgccgac agccgcgacc cggactttgc agcgggaaaa
     1201 gaaagcgggg agaaagcaca tactcttacc aaggcagaac tgccaaatgt ctctatcggt
     1261 ctcggggcag cagtgattaa aagcgatacg gaagctatcc gcgttaccga ctcaggcccg
     1321 ctctggtatc gtatgggaag atctgtacag gagacggccg gacctctggg caacggcgag
     1381 cctcataaca atctccctcc ctaccgcgcg gtgtacatcc agcttcgcgc caagcccgac
     1441 ccggtggtgg cgcaggaggt ggcggcggac aggctggcca cagcgcgcag gatcggcagc
     1501 gccagctttg acggcagcgc ggatatcacg ctggcacaga tgggggtcat aacacagagc
     1561 aatatgacat tgcatgtctc tcctacaggc aacgacaata cgggaaaagg gacacagggg
     1621 gccccttata aaacgatcgc acgggctatc caagatgtgc cgcagattgt caatcacacc
     1681 ataacgatca gtttggcacc cggaaattac ggcagtgaaa caatttcttt agggatgtca
     1741 gggaaaggcg ctttgcagat tgtaggatct ggaaacagtc ttgcaaatgc tacaaattat
     1801 aagttcggat atttgagatt ctttaatttc gcctgctctt taaatattta tgggatcagt
     1861 acgacttctt cccctgattg gggcggctcg ctgcaatttg agaactgttc tcatgtcttc
     1921 gtgcagtatt gtgactgcca agctggaaag gacaatggaa aagctggtgt ttatatggat
     1981 aagggttatt gctacgtcca tacctgcaat tttcatggtt gctcaagtgc agtgttcgcg
     2041 gtacgtggag gagttatggc ggtaaccaat tccacgggta ataacaatac cacgggattt
     2101 aacgcatcaa tgggcggtgt tgcagtaaaa atggttgacc agaccaacaa tcttggcggg
     2161 acgaacgcaa caagtgcata caacggcggc attatcgtgg ggccgggcgg aggaatcatt
     2221 gggaacattt aaggataagg agaaatgtag atggaaatca agggaatcga tgtgagcaga
     2281 tatcaaaacg ccatcgactg gc
//
LOCUS       DBMDYA010000487         2299 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9858_length_2299_cov_1.270701, whole genome shotgun sequence.
ACCESSION   DBMDYA010000487 DBMDYA010000000
VERSION     DBMDYA010000487.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_2299_cov_1.270701"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="cmk"
                     /locus_tag="ACZPFK_10955"
     CDS             <1..586
                     /gene="cmk"
                     /locus_tag="ACZPFK_10955"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1406875.1"
                     /translation="LYVDTGAMYRAIGLYMLRAGVDTSDAAAVLPRLGEVRLELKYLD
                     GTQHVFLNGEDVSEEIRREEVSMAASSVSAHQGVRDFLTETQRSIAQRNNVLMDGRDI
                     GTIILPNAQIKIFLTASAEERARRRTRELREKGQPAEYEEVLADIRRRDYNDSHRAAA
                     PLRQAKDAVLVDTSDDDFEAAYQKLYRLIQGRLG"
     gene            588..1211
                     /locus_tag="ACZPFK_10960"
     CDS             588..1211
                     /locus_tag="ACZPFK_10960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1406876.1"
                     /translation="MFWFYHLCFTLASVIFRLPYRIRVRGRENLPRDRGYVLCPNHLS
                     AIDPVFVVLARGFGKPMWVMAKEELFHNRFVAWFFRHVGVFPVERGKGDKTVLNNAIE
                     SVRNGRSMLIFPEGTRNKKGEGLLPLKSGAFVVAEAAHADIVPCRVLYRGGRQHLFCT
                     VTVVFGAPIPIDSLNLGGEHAASKLRAAKAVLASRLGELYEQNKQYE"
     gene            1267..>2299
                     /gene="ispH"
                     /locus_tag="ACZPFK_10965"
     CDS             1267..>2299
                     /gene="ispH"
                     /locus_tag="ACZPFK_10965"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR1406877.1"
                     /translation="MGIIRAKTAGFCFGVDRAVRMTEQLAREGRRVATLGPLIHNPQC
                     VAALEAQGVVTADSVGDIPQGYEVVIRSHGVGEDVYAALAEKGAVVHDATCPFVAKIQ
                     RIAAEAGRAGATLLIAGDRDHPEVQGIVGHACGEVHVFADLEALEACSTPQLRQKPAF
                     VVAQTTFEVTKWLESTKFIKKVYTSPRIFDTICSATWARQQEAEELARRCDLMVVIGG
                     RRSSNTQKLVRVAGKHCKTLAVETAAEITAGDFAGVRRAGVTAGASTPSSIIEEVLNK
                     MSEVVQNEEMSFDEMIDQSLKPVFNGKVVTGIVTGISPSEITVDIGTKQTGFVAASEW
                     SDDPTVKLED"
BASE COUNT          432 a          666 c          808 g          393 t
ORIGIN      
        1 cctctatgtg gacaccggcg ccatgtaccg ggccattggc ctgtatatgc tgcgcgcggg
       61 cgtggatacc tcggacgcgg cggcggtgct gccccgcctg ggagaggtgc gcctggagct
      121 gaaatatctt gacggcacgc agcacgtgtt tctcaacggg gaggacgtgt cggaggagat
      181 ccggcgcgaa gaggtctcga tggcggccag cagcgtgtcc gcccatcagg gggtgcggga
      241 ctttctcacc gagacccagc gctccatcgc gcagcggaac aacgtgctca tggacggccg
      301 ggatatcggc accatcatcc tgccgaacgc ccagatcaag atatttttga ccgccagcgc
      361 cgaggagcgg gcccgccgcc gcacccgcga gctgcgggaa aagggccagc cggcggagta
      421 tgaggaggtg ctggcggaca tccgccggcg cgactacaac gacagccacc gcgccgccgc
      481 gcccttgcgg caggcaaagg acgcggtgct ggtggatacc agcgacgatg atttcgaggc
      541 cgcgtaccaa aagctgtacc ggttgatcca ggggaggctt ggctgaaatg ttttggtttt
      601 atcacctctg cttcaccctc gcctcggtga tcttccggct gccctaccgc atccgggtgc
      661 gcggcaggga aaacctgccc cgggaccggg gctatgtgct ctgccccaac catctctccg
      721 ccatcgaccc ggtgttcgtg gtgttggcgc gggggttcgg caagcccatg tgggtcatgg
      781 ccaaggagga gctgtttcac aaccggtttg tggcctggtt cttccggcat gtgggcgtgt
      841 tcccggttga gcggggcaaa ggggacaaga cggtgctgaa taacgccatc gaatcggtgc
      901 gcaacggccg ctcgatgctg atcttccctg aggggacccg caacaaaaag ggggaggggc
      961 tgctgccgct caagagcggc gctttcgtgg tcgccgaggc ggcccacgcc gacatcgtgc
     1021 cctgccgggt gctctaccgg ggcggcaggc agcacctgtt ctgcaccgtg accgtggtat
     1081 tcggcgcgcc catccccatc gacagcctga acctgggcgg cgagcacgcg gccagcaagc
     1141 tgcgggcggc caaagcggtg ctggcgtccc gtttgggcga gctgtacgag cagaacaaac
     1201 aatacgaatg agcgggcggg gagggtgctt tccacagggg gagcccccct gcccgggagg
     1261 aaatttatgg ggatcatccg tgcaaagacc gcgggcttct gctttggggt ggaccgcgcg
     1321 gtgcgcatga ccgagcagct ggcccgagag gggcgcaggg tcgccacgct gggcccgctg
     1381 atccacaatc cgcagtgcgt cgccgcgctg gaggcgcagg gcgtcgtgac ggcggattcg
     1441 gtgggggata taccgcaggg ctacgaggtg gtcatccgca gccacggcgt gggcgaggac
     1501 gtctatgccg cgctggccga aaagggcgcg gtggtgcacg acgcgacctg cccctttgtg
     1561 gccaagatcc agcgcatcgc ggccgaggcc ggacgggcgg gtgcgacgct gctcatcgcg
     1621 ggcgacaggg accatccgga ggtgcagggc attgtgggcc acgcctgcgg ggaggtgcac
     1681 gtttttgccg atttggaggc gctggaggcc tgctccaccc cacagctgcg gcaaaaaccg
     1741 gcatttgttg tagcccagac gacttttgaa gttacaaaat ggttagaaag cacaaagttt
     1801 ataaaaaaag tgtatacaag cccgcgaatt tttgatacaa tatgtagtgc aacgtgggcg
     1861 aggcagcagg aggcggaaga gctcgcgcgc agatgcgacc tcatggtggt catcggcggg
     1921 cggcgttcct ccaacaccca gaagctggtt cgtgtcgcag gcaagcactg caagacgctg
     1981 gcggtggaga ccgcggcgga gatcaccgca ggcgattttg cgggggtgcg gcgtgccggc
     2041 gtcacggcgg gggcttccac gccgtcgtct ataattgagg aggtacttaa caagatgagc
     2101 gaggtagtac aaaacgaaga gatgagcttt gatgagatga tcgatcagtc cttgaagccg
     2161 gtcttcaacg gcaaggtcgt gaccggtatc gtcaccggaa tctcgccgag cgagatcacc
     2221 gtggacatcg gcaccaagca gaccggcttt gtcgccgcgt ccgagtggtc tgacgatccc
     2281 accgtgaagc tggaagatg
//
LOCUS       DBMDYA010000488         2294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9886_length_2294_cov_1.162791, whole genome shotgun sequence.
ACCESSION   DBMDYA010000488 DBMDYA010000000
VERSION     DBMDYA010000488.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_2294_cov_1.162791"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            254..1573
                     /gene="rsxC"
                     /locus_tag="ACZPFK_10970"
     CDS             254..1573
                     /gene="rsxC"
                     /locus_tag="ACZPFK_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR1406878.1"
                     /translation="MIKNVFKRAAKGAHVPHNKNTQNTMTVDMPVPEKILLPMQQHIG
                     GTAVPMVKKGDPVFVGTLVGRAEGYISAPIHSGVSGTVTDVREIQISSGARVPAVEIT
                     SDGMQTPDPAIAPPAVTDYESFTTAIRASGLVGLGGAGFPTSVKLTMKDLSVIDTLII
                     NGAECEPYITADNREFIENPEDVMSGIVAVKKWLNIKSVIIAIERNKPEAMDVMFGLI
                     KGDPSITVKPLPSVYPQGAEKILIESCTGREVPRGGLPSDVGCIVMNVSSVAFVAAYL
                     RTGMPLTSRRLTVDGGAVAEPKNVRVIVGTPIADVLAFCGGTKGEVGEIITGGPMMGV
                     SVVDTAFPILKQNNAVLAFPRAEAELPPVAECIHCGRCIEACPVGLSPVIIAKAYNAH
                     NLPELDKLMVDLCMECGCCSFVCPAKRPVTETMRSAKAMLKKGAKKK"
     gene            1573..>2294
                     /locus_tag="ACZPFK_10975"
     CDS             1573..>2294
                     /locus_tag="ACZPFK_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1406879.1"
                     /translation="MEHTNMLVKASPHIRDNSSTQKIMLDVIIALCPAIVASGIIFGW
                     RALLVIGVTAAASVAFEYLYCYLMKKPITISDLSAVVTGILLAFNLPSTIPLWIAILG
                     SFVAVVIAKQMFGGLGHNFANPAIVGRIVLAVSFAGRMTAYAYPAVPGGVDALAGATP
                     LVATQHADLPLLQLFLGAHGGVLGETCAAALLLGGVYLVARRVISPAIPVTYLATVAL
                     VSLLAGQEPLVQLLLPGQQADQG"
BASE COUNT          420 a          764 c          732 g          378 t
ORIGIN      
        1 tctgcaggaa acaccgcgaa aaacagagaa aagggcgaac cccatgccgg tgcggacatt
       61 ttggaaaaaa tgtccgcacc gggtggccgg aaagccgcca tttaccggga ttttctgatt
      121 gtaaatgcgc ggaaaacaag gtataataag ggctggtctg accatccccc gggcgcggcg
      181 ccccggatgg cggtcggcgc cgcagcccgc ggccccgccc agacgagaga aaaagaacag
      241 gaggaacgtt cacttgataa aaaatgtttt caagcgtgcg gcaaagggcg cccatgtgcc
      301 ccacaacaag aacacgcaga ataccatgac agtggacatg ccggtgccgg agaagatcct
      361 gctgcccatg cagcagcaca tcggcggcac ggcggtgccg atggtgaaaa agggcgatcc
      421 ggtctttgtg ggcaccctcg tgggccgggc ggaggggtat atctccgcgc ccatccactc
      481 gggcgtatcc ggcacggtca ctgacgtgcg ggagatccag atctccagcg gcgcgcgggt
      541 gcccgcggtg gagatcacct ccgacggcat gcagaccccg gaccccgcca ttgccccccc
      601 ggcggtgacc gactatgagt ctttcaccac cgccatccgc gccagcggac tggtgggctt
      661 ggggggcgcg ggctttccca cctcggtcaa gctgaccatg aaagacctgt cggtcatcga
      721 caccctgatc atcaacggcg ccgagtgcga gccctatatc accgccgaca accgcgagtt
      781 catcgagaac cccgaggacg tgatgagcgg catcgtggcc gtgaagaaat ggctgaacat
      841 caaaagcgtc atcatcgcca tcgagcgcaa caagcccgag gccatggacg tgatgttcgg
      901 cctcatcaag ggcgacccct ccatcaccgt caagcccctg cccagcgtct acccgcaggg
      961 cgcggagaag atcctcatcg agagctgtac cggccgcgag gtgccccggg gcggcctgcc
     1021 cagcgatgtg ggctgcatcg tgatgaacgt gtccagcgtg gcctttgtgg cggcctatct
     1081 gcgcaccggc atgccgctga cctcccgccg cctgaccgtg gacggcggcg ccgtggccga
     1141 gcccaaaaac gtgcgggtca tcgtgggcac ccccatcgcc gacgtgctgg ccttttgtgg
     1201 cggcaccaag ggcgaggtgg gcgagatcat caccggcggc ccgatgatgg gcgtctcggt
     1261 ggtggacacc gctttcccca tcctcaaaca gaacaacgcc gtgctggcct ttccgcgcgc
     1321 ggaggccgag ctgccgccgg tggccgagtg catccattgc ggccgctgca tcgaggcctg
     1381 ccccgtgggc ctgtcgccgg tgatcatcgc caaggcatac aacgcccaca acctgcccga
     1441 gctggacaag ctgatggtgg acctgtgcat ggagtgcggc tgctgcagct ttgtctgccc
     1501 ggccaagcgc ccggtcaccg agaccatgcg ctccgccaag gccatgctga agaaaggggc
     1561 gaagaaaaag tgatggaaca cacgaatatg ctggtcaagg cttcgcccca tatccgggac
     1621 aattcctcga cccaaaagat catgctggac gtgatcatcg cgctctgccc ggccatcgtc
     1681 gccagcggca tcatcttcgg ctggcgggcg ctgctggtca ttggcgtgac cgccgcggcc
     1741 agcgtggctt ttgagtacct gtactgctac ctcatgaaaa agccgatcac catcagcgac
     1801 ctgtccgccg tggtcaccgg catcctgctg gcgttcaacc tgccctccac gatccccctg
     1861 tggatcgcca tcctgggcag ctttgtggcc gtggtcatcg caaagcagat gttcggcggc
     1921 ctgggccaca acttcgcaaa cccggccatc gtaggccgca tcgtgctggc cgtcagcttt
     1981 gcgggccgca tgaccgccta tgcctacccg gcggtgccgg gcggcgttga cgcgctggcc
     2041 ggggccaccc cgctggtggc cacccaacac gccgacctgc cgctgctgca gctctttttg
     2101 ggcgcgcacg gcggggtgct gggcgagacc tgcgccgcgg cgctgctgct gggcggcgtc
     2161 tatctggtgg cgcggcgggt catctccccg gccatcccgg tgacctatct cgccaccgtg
     2221 gccctggtca gcctgctggc cgggcaggag ccgctggtgc agctgctcct gcccggccag
     2281 caggctgacc aggg
//
LOCUS       DBMDYA010000489         2292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9892_length_2292_cov_1.396623, whole genome shotgun sequence.
ACCESSION   DBMDYA010000489 DBMDYA010000000
VERSION     DBMDYA010000489.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9892_length_2292_cov_1.396623"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            219..1094
                     /locus_tag="ACZPFK_10980"
     CDS             219..1094
                     /locus_tag="ACZPFK_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1406880.1"
                     /translation="MSIQNLSGKRVAKDAFTLCAVLASSLLQTYTIQTFMTPAKLISS
                     GFTGVAQLLNMLTEGLAFHVDTSLAIILLNVPVALLCARGISPRFTLFSCIQFGCTSL
                     FLKVLHFSPILNDVILNVAFGGFLYGLSVVVALKGNASTGGTDFVALYFSNKFGKSLW
                     QYVFIFNTVVVLIFGASRGWLYAGYSIVFQFISTKTISTFHTRYERLTMQITTERAEE
                     LLQAYTAHYRHGVSCVDGYGGYSRQPFTVLTTVVSSYEVKEIIHLMKEVDPKVIINIY
                     RTEDFVGGFYQKPID"
     gene            complement(1704..>2292)
                     /locus_tag="ACZPFK_10985"
     CDS             complement(1704..>2292)
                     /locus_tag="ACZPFK_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1406881.1"
                     /translation="AVRKPMFYLAWLTCLCYSAASSVAGYAATFTTMELGQSIRFGSL
                     ATACVSLGMIGCGFLLGVLNDRFGARAGLIWGAVFNVIGFSGMILSIRNVSFLLPSCT
                     VLGLGGSMYTVQAPLIARSVLGPRHYSEIWSVMMIGNSLMGALSFGPMGLFYDKTGSY
                     LGAFLLAMALFTAALFTGWAALSLSARFRAKAAAE"
BASE COUNT          483 a          696 c          664 g          449 t
ORIGIN      
        1 ccgcggtcct gtgctaaaat gaaaaacgaa gagactgggg cggctgggct ggaaccgggc
       61 aatcaagccc ggctttcagg ctatgatccc ccgacgtgcc tccggccgag gcaaaagctc
      121 ttgttccgga gcgctttttc tcccaagcct ctgctgcggc ccgcgcggcg gggcaaacgg
      181 ccgagtcctc tttttgaaac agaaaaggaa gatgtgcgat gagcattcaa aacttgagcg
      241 gcaaacgggt ggcgaaagac gcctttaccc tttgcgcggt gttggcgtcc tcgctgctgc
      301 agacctatac catccagacg ttcatgaccc cggcaaagct gatctccagc ggttttaccg
      361 gcgttgccca gctgctgaac atgctcaccg aggggctcgc ttttcatgtg gacacctcgc
      421 tggccatcat cctgctgaac gtgccggtgg cgctgctctg cgcccgcggc atcagcccgc
      481 gttttacgct gttcagttgc atccagttcg gctgcacctc cctcttttta aaggtgctgc
      541 acttcagccc catcctgaac gacgtcattt tgaatgtggc gttcggcggc tttctctacg
      601 gcctgagcgt ggtcgtcgcc ctcaagggca acgcttccac cggcggcacc gattttgtgg
      661 cgctgtattt ttccaataaa ttcggcaaat cgctgtggca gtatgtgttt atcttcaaca
      721 ccgtggtggt gctgatcttc ggcgcctccc gcggctggct ctacgccggt tattccatcg
      781 tcttccagtt tatctccacc aagaccatct ccactttcca cacccggtac gagcggctga
      841 cgatgcagat caccaccgag cgcgcggagg agctgctgca ggcctacacc gcccattacc
      901 ggcacggggt ctcctgcgtg gacggttacg gcggctattc ccgtcagccg ttcaccgtgc
      961 tgaccaccgt ggtctcctct tatgaggtca aggagatcat ccacctgatg aaagaggtgg
     1021 accccaaggt gatcatcaac atctaccgca ccgaggattt tgtgggcggt ttttatcaaa
     1081 aacccatcga ctgaaaaaag ctagccgcat agcggctagc tttttttatc gctccgcgat
     1141 aaaaaacaaa ttattttaat ggccccggcc cagggatggg ccggatggcc gaaaaaatag
     1201 cctgcggcta ttttttatag ctttgcatcg caaagctata aaaacggcta tgtgggccgt
     1261 cccggcccac ggccttaaaa tcttgagcgc ccgcccaagg gagtgggcgg atgcgcgtaa
     1321 aaaatagcct gcggctattt tttattgctc cgcaataaaa aacgacttat ctctatggcc
     1381 ccggccggag gaccggccgg atggccgaaa aaatagccgc acagcggcta tttttgatga
     1441 tttgcgccgg tcgcagcacg ggtcggccgc atcgggcgga aagaagagag aaagcggcgt
     1501 cccgttttca cgccgttccg gcagggaacg actgccgcct gattctgacg gaaaagtccg
     1561 cctgcaagga agcggaacgc cgcaatcctc tctggcccgc aagcgtcgct gacgcggaag
     1621 agcggacctt tcccgagaac agccaaggct gcgcagtctt gccctgccgg gcaaagccga
     1681 acctctcccg tcctcgccgg ggtttattcc gccgcggcct ttgcgcggaa acgggcgctc
     1741 agagaaagtg cggcccagcc ggtgaagaga gcggcggtaa aaagggccat ggccagcaga
     1801 aaggccccca gataagaccc tgtcttgtca taaaagagtc ccatgggccc aaaggagagc
     1861 gcgcccatca ggctgttgcc gatcatcatg accgaccaga tctccgagta gtgcctgggc
     1921 cccagcacgc tgcgggcgat cagcggcgcc tgcaccgtgt acatgctgcc cccgaggccc
     1981 agcaccgtgc aggagggcag caaaaaggag acgttgcgga tggacaggat catgccggaa
     2041 aagccgatca cgttgaagac ggccccccag atgagcccgg ctctggcgcc gaagcggtcg
     2101 ttgaggaccc ccaggaggaa gccgcagccg atcatgccca agcttacaca cgcggtggcg
     2161 agagagccga accggatgct ctgccccagc tccatcgtgg tgaaagtggc ggcgtaaccc
     2221 gctacgctgg aggccgcgct gtagcacagg caggtcagcc aggcgagata aaacatgggc
     2281 ttgcgcaccg cc
//
LOCUS       DBMDYA010000490         2291 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9902_length_2291_cov_1.472146, whole genome shotgun sequence.
ACCESSION   DBMDYA010000490 DBMDYA010000000
VERSION     DBMDYA010000490.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9902_length_2291_cov_1.472146"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2025)
                     /locus_tag="ACZPFK_10990"
     CDS             complement(<1..2025)
                     /locus_tag="ACZPFK_10990"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR1406882.1"
                     /translation="MITVTGTVFQLDTAHTSYLFEARDGLLQQLYYGPRIKTHDVEPL
                     REKTAAGYGSEVVYKAAAKPLSLDSRPLEFSPKNKGDYRLPAFSARTQAGFTADFVYE
                     GFRVEEGLPAPEGLPAPHGADGCLAVTLKTADGLEVELFYGVFEQADVIARSLRFING
                     SRAPVQLLRALSFQLDLPGCGWTLATLDGAWARERHVTEHRLAPGVVAFGSRTGASSN
                     RTNPFFMIYEDGAGEFAGRVYGCNLVYSGSFEGAAEVSPLGSTRIMEGVQSDGFYWPL
                     APGERFEAPWAVLTCSDAGKNGMSGNMHRFVKEHILPPAFAHKLRPVLLNNWEATYFD
                     FNERRLLKLAREAAALGVELFVLDDGWFGARDSDSAGLGDYEVNRKKLPSGLDGLGDK
                     VRALGMRFGLWMEPEMVNEDSDLYRAHPDWAVKTPGVEPGRGRNQLVLDLCRGEVRDY
                     IVENVVRTIKTAGAAYVKWDMNRHVSDNYSPALDEQGRFAHRFVQGLYEVLRRVTEAC
                     PEVLFEGCASGGDRFDLGVLCFMPQIWTSDDTDAYERQLIQTGTSYGYPPCVMAAHVS
                     ASPNHQASRPSPLEARFDVAAFGLLGYELDLTVASAAEKKAMKEQIAWYKAHRRVLQQ
                     GDFYRLKSPFGPGDTRGLTARETQWIAVSPDKSEAVAGEFMGLLVPSSAKP"
BASE COUNT          389 a          792 c          714 g          396 t
ORIGIN      
        1 cggctttgcg ctgctgggca ccagcaggcc catgaactcg cccgccaccg cctcgctctt
       61 gtccggcgag acggcgatcc actgggtctc ccgcgcggtc aggccgcggg tgtcgcccgg
      121 cccgaacggg cttttcagcc ggtaaaaatc gccctgctgc agcacccggc ggtgcgcttt
      181 ataccaggcg atctgctctt tcatcgcctt tttctccgcc gccgaggcga cggtgaggtc
      241 cagctcatag cccagcagcc caaaggccgc cacgtcgaac cgcgcctcca ggggcgaagg
      301 gcgggaggcc tggtggttgg ggctggccga gacgtgggcc gccatcacac aggggggata
      361 gccgtagctg gtgccggtct ggatgagctg gcgctcatag gcgtcggtgt cgtcgctggt
      421 ccagatctgg ggcatgaagc agagaacccc caggtcaaag cggtcgccgc cggaggcgca
      481 gccctcgaac agcacctcgg gacaggcctc ggtgacgcgg cgcaagacct catacagccc
      541 ctggacaaag cggtgggcga accggccctg ctcgtccagg gccggggaat agttgtcgga
      601 cacatgccgg ttcatatccc acttcacata ggcggcgccg gccgtcttga tcgtgcgtac
      661 cacgttttcg acgatgtagt cgcgcacctc cccgcggcag aggtccagca ccagctggtt
      721 gcggccgcgg cccggctcca cgccgggggt cttcacggcc cagtccgggt gggcgcggta
      781 gaggtcggaa tcctcgttga ccatctccgg ctccatccag agaccgaacc gcatgcccag
      841 cgcccgcacc ttgtccccca gcccgtccag gccggagggc agttttttgc ggttcacctc
      901 atagtcgcca aggcccgcgc tgtcgctgtc ccgggcgccg aaccagccgt cgtccagcac
      961 gaacagctcc acgcccaggg ccgctgcctc ccgggccagc ttcagcaggc ggcgctcgtt
     1021 gaaatcgaaa taggtggcct cccagttgtt gagcagcacc gggcgcagct tatgggcaaa
     1081 ggccgggggc aggatatgct cctttacaaa gcggtgcata ttgccgctca tgccgttttt
     1141 gcccgcgtcc gagcaggtca gcacggccca gggcgcctcg aaccgctccc ccggggccag
     1201 cggccagtag aagccgtcgc tctgcacccc ctccatgatg cgggtggagc ccagcggcga
     1261 gacctcggcc gcgccctcaa agctgccgga gtagaccagg ttgcagccgt agacccgccc
     1321 ggcaaactcg cccgccccgt cctcgtagat cataaaaaac gggttggtgc ggttggagga
     1381 ggcgccggtg cggctgccga acgccaccac gccgggggcc aggcggtgct cggtgacatg
     1441 gcgctcccgc gcccaggcgc cgtccagcgt cgccagcgtc cagccgcagc ccggcaggtc
     1501 cagctgaaag ctcagggcgc gcagcagctg caccggcgcg cgggagccat tgataaagcg
     1561 caggctgcgg gcgatcacgt ccgcctgctc aaacacgcca tagaacagct ccacctccag
     1621 gccgtccgcc gttttcaggg tcaccgccag gcagccgtcc gcgccatggg gggcgggcag
     1681 cccctcgggc gcgggcagcc cctcctccac gcgaaaaccc tcgtagacga aatcggccgt
     1741 aaagccggcc tgcgtgcgcg ccgaaaaagc gggcagccgg tagtccccct tgtttttggg
     1801 cgagaactcc agcggtcggc tgtccagcga gagcggcttt gccgccgcct tatagaccac
     1861 ctcgctgccg tagcccgccg ccgtcttttc ccgcagcggc tccacgtcgt gggtcttgat
     1921 cctgggcccg taatagagct gctgcagcag gccgtcgcgc gcctcaaaca gatagctggt
     1981 atgcgccgtg tccagctgaa ataccgtgcc ggtgaccgtg atcacaagcc gcgccccctt
     2041 tgtcgccgaa ttttgttcca ccaggcattt tggggccgtt gacaaagcca tttcttaaaa
     2101 acagggaagc tttgttgcgg cctgttgcac aaaaatactc gttaagccgg aaaagctgcg
     2161 ttttttgttt catccgccaa aagattcgtc tgcgttttcc gcgcccctca gtttgtcaac
     2221 agccccattt tgtctccggt tcaattatac acccctaccg gatggtaaaa caaccgccgt
     2281 tgtgctatac t
//
LOCUS       DBMDYA010000491         2290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9910_length_2290_cov_1.378712, whole genome shotgun sequence.
ACCESSION   DBMDYA010000491 DBMDYA010000000
VERSION     DBMDYA010000491.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9910_length_2290_cov_1.378712"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="ACZPFK_10995"
     CDS             complement(<1..1089)
                     /locus_tag="ACZPFK_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1406883.1"
                     /translation="MANGQKQRQNGEAVRAGGGITGYHRAIRRKWAVLAGLFAAMLFF
                     MILSVNAGSAAISPWEVLKTVVGAGDERGFVVVWRIRMPRVVAAVVAGAGLSIAGCVM
                     QTCLKNPLASPSTLGVSAAATFGANVAIIGLGAGAVLSTASDAVAINNPYLVTVCAFV
                     CSFAAILLILALSKLKGFSPESIVLAGVALSSLFSAGTTLIQYFGDDVAIAAALFWTF
                     GDLGRISWPEVGILAAVTAAAILYFLLMRWDYNALANGEETAKSLGVKTFRVRFWGLM
                     VSALLTAVSVSFMGMIGFIGLIGPQIMKRVVGADHRFLIPASALMGAVILLLADTCAR
                     TVISPIILPVGAITSLLGAPMFFYMLLKG"
     gene            complement(1089..1604)
                     /locus_tag="ACZPFK_11000"
     CDS             complement(1089..1604)
                     /locus_tag="ACZPFK_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012481.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1406884.1"
                     /translation="MRNSVIYFSETGNTEAIARFVADDARSVEGMEARLFRPAEGPDK
                     SFVDESAAVRFGTPTHCASLCWQMKKWFDTEWDVRLGGRLGAAFATENSPNGGGAEAA
                     ILSVIPHLLVRGMPAHSSVAERGRPFIHIGPAAVRDQIAEKEELCGLFGRRVALKADK
                     LFGASSRRGAR"
     gene            complement(1601..>2290)
                     /locus_tag="ACZPFK_11005"
     CDS             complement(1601..>2290)
                     /locus_tag="ACZPFK_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1406885.1"
                     /translation="EGDEFLRSLRQKPPLTPKMRVLDVGCGAGAYALALASQAAEVVG
                     VDFSPKMIEAARRSAARAHRANVSFLERDWHRCSSEEFRGRFDLAFAHTTPAVADYAT
                     LVKMMEASKRYCALCKPARRSDQVFDRIREIAGCAGRENDASVAYAFDTIWGHGYDPE
                     VTYSKAVWQSRRTAAEAEKWYLGRLKGSCALTAAAEEEIRRYLAAISVDGFVTERTET
                     TLVNFFWEMNG"
BASE COUNT          434 a          786 c          705 g          365 t
ORIGIN      
        1 tcctttcagc agcatataga aaaacatcgg cgcgcccagc agcgaggtga tggcccccac
       61 cggcaggatg atgggcgaga tcaccgttct ggcgcaggtg tcggccagca gcaggatcac
      121 ggcccccatc agcgcggagg cagggatgag aaagcggtga tcggcgccca cgacgcgctt
      181 catgatctgc ggcccgatga ggccgataaa accgatcatg cccataaacg acacggacac
      241 ggcggtgagc agggcggaga ccatcaggcc ccaaaagcgc acgcggaacg tctttacccc
      301 caggcttttg gccgtctcct ccccgttggc gagcgcgttg tagtcccagc gcatcaacag
      361 aaaatagagg atcgccgcgg ccgtgacggc cgccaggatg cccacctccg gccaggagat
      421 cctgcccagg tcgccaaagg tccaaaacag ggccgcggca atcgccacat cgtcgccgaa
      481 atactggatc agagtggtgc ctgcgctgaa cagcgagctg agcgccacgc cggccagcac
      541 gatggactcg ggtgaaaacc ctttcagctt tgagagggcc aggatcagca ggattgccgc
      601 gaaagagcag acaaaggcgc agaccgtgac cagatacgga ttgttgatcg ccaccgcgtc
      661 ggaggcggtg ctcagcacag cccccgcccc gaggccgatg atcgccacgt ttgcgccaaa
      721 ggtcgccgcc gccgagaccc cgagagtgga gggcgaggcg agcgggtttt tcaggcaggt
      781 ctgcatcacg cagccggcaa tcgaaaggcc cgcccccgcg accaccgccg cgaccacacg
      841 gggcatacgg atgcgccaga cgaccacaaa gccgcgctcg tcccccgccc cgaccacggt
      901 cttgagcacc tcccaggggg agatggccgc ggagccggca ttgacggaaa ggatcataaa
      961 aaagagcatc gcggcaaaga ggccggcgag gaccgcccat ttgcggcgga tggcgcggtg
     1021 atatcccgtt atgcctccgc ctgcccgcac ggcctcaccg ttctgccgct gtttttgtcc
     1081 gtttgccatc accgtgcccc ccggcgggaa gaagccccga acaatttatc ggctttcagc
     1141 gcgacccgtc ggccgaacag cccgcacagt tcttcttttt cggcgatctg gtcccgcacc
     1201 gccgcgggcc cgatatggat gaaagggcgg ccgcgctctg cgacgctgga atgggccggc
     1261 atgcccctga ccagaagatg ggggattaca ctcaggatgg ccgcctccgc cccgccgccg
     1321 ttgggggaat tttcggtggc gaacgccgcc cccagcctgc cccccagccg cacgtcccac
     1381 tcggtgtcga accatttttt catctgccag cacaggctgg cgcagtgggt cggggtgccg
     1441 aaccgcacgg cggcgctctc gtcgacaaag ctcttgtcgg gcccctctgc cggacggaaa
     1501 agccgcgcct ccatcccctc cacgctgcgg gcgtcgtcgg ccacaaagcg ggctatcgct
     1561 tcggtgttgc ccgtctcgct gaaataaatg acgctgttcc tcatccgttc atctcccaga
     1621 aaaaattgac gagcgtggtc tcggtcctct ccgtcacaaa gccgtcgaca ctgatcgcgg
     1681 caagatatct cctgatctcc tcctcggccg cggccgtgag agcgcagctg cctttcagtc
     1741 gccccagata ccacttctcc gcctctgcgg ccgtcctgcg ggactgccag acggctttgc
     1801 tgtaggtcac ctccgggtca tagccgtggc cccagatcgt gtcgaacgca taggcgacgg
     1861 aggcgtcgtt ctcgcgcccc gcacagcccg cgatttcacg gatgcggtcg aacacctggt
     1921 ccgagcggcg ggcgggcttg cacagggcgc agtaccgctt tgacgcctcc atcatcttca
     1981 ccagcgtggc atagtccgcg acggccgggg tggtgtgggc gaacgccagg tcaaatcttc
     2041 ctctgaattc ctccgaagaa cagcggtgcc aatcccgctc caaaaaggac acgttggccc
     2101 ggtgcgcccg cgcggcgctg cgccgggccg cctcgatcat tttgggtgaa aaatccaccc
     2161 ccaccacctc cgccgcctgc gaagccaggg caagggcgta agcgcccgcg ccgcagccga
     2221 cgtcgagaac acgcatcttt ggggtgagcg ggggcttttg ccgcaggctg cgcaggaact
     2281 cgtcgccctc
//
LOCUS       DBMDYA010000492         2282 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9946_length_2282_cov_1.338835, whole genome shotgun sequence.
ACCESSION   DBMDYA010000492 DBMDYA010000000
VERSION     DBMDYA010000492.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_2282_cov_1.338835"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(102..464)
                     /locus_tag="ACZPFK_11010"
     CDS             complement(102..464)
                     /locus_tag="ACZPFK_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1406886.1"
                     /translation="MANALDQFMDAAREVANKAVDAAGNLAAKGKDKLDRMSLENELA
                     KAQRQLGALVYSLRKSGEENPDLVEHYIEVIADVEAKLNEHEAAQAERYCVSICPECG
                     AEVGEDAGFCAHCGAKLS"
     gene            complement(626..1369)
                     /locus_tag="ACZPFK_11015"
     CDS             complement(626..1369)
                     /locus_tag="ACZPFK_11015"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR1406887.1"
                     /translation="MSCRPARPGAFCCKNGSYSCVISLDNFFKNLEDYPRLPDGSKDY
                     ENVQAIDVPLVNRCLSELLETGRTRIPDFDFLSERRKEEWLDLDLNGGVVVVEGIHAL
                     NPALTASLPRSKVYKIYAGLREEYSYRGQRVLPTRDIRLARRMVRDYKFRGHSLEKTL
                     GMWDNVCIGEDQFIKVFKPEADLLLDTSFSYEICCLAPSITPLAGKLPENSPYAERLY
                     QLAGAFSLCAPVDEGLIPADSMLQEFLGE"
     gene            1524..1599
                     /locus_tag="ACZPFK_11020"
     tRNA            1524..1599
                     /locus_tag="ACZPFK_11020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1557..1559,aa:Ala,seq:cgc)
BASE COUNT          431 a          688 c          712 g          451 t
ORIGIN      
        1 taaaactttc catgtccttt tgtgccccat gcacaggtca gcaggcgggg ccacggcccg
       61 gccgacgcgc cttttccagg taccagaagc gggccctgcc gtcaggacag cttagccccg
      121 cagtgagcgc aaaacccggc atcctccccg acctccgcgc cgcattcggg gcagatcgaa
      181 acgcagtagc gctccgcctg ggcggcctca tgctcgttga gcttcgcctc cacgtcggcg
      241 atgacctcga tatagtgttc caccaggtcg ggattctcct cgccggattt ccgcagtgag
      301 tagaccagtg cccccagctg gcgctgtgcc ttggccagct cgttttccag gctcatgcgg
      361 tccagtttgt ccttgccctt ggcggccagg tttccggctg cgtccacagc cttgttcgcc
      421 acctcccggg cggcgtccat aaattgatcc agtgcgtttg ccattgtgcg tacccctctc
      481 tcttagtatg gccccccgca cggcccttaa acgccgctgg gagggcggta tccgcagatc
      541 tgcgcctcgg gggcgggttc cccgcaggag gcctccccca ttatagcggt gccggcccca
      601 atctgcaacc ggtccgccgg aaaattcact cgcccaaaaa ttcctgcaac atcgaatccg
      661 ccgggatcag cccctcgtcg acgggggcac agagcgaaaa cgccccggcc agctggtaga
      721 ggcgctccgc ataggggctg ttctccggca gcttgcccgc cagcggcgtg atggagggcg
      781 ccaggcagca gatttcatag ctgaaagagg tgtccaacag caggtcggcc tccggcttga
      841 aaaccttgat gaactggtcc tcgccgatgc agacgttgtc ccacatgccc aaggtttttt
      901 ccaggctgtg gccgcggaac ttgtaatccc gcaccatgcg ccgcgccagg cggatgtccc
      961 gggtgggcag cacccgctgg ccgcgatagg agtattcctc ccgcaggccg gcgtagatct
     1021 tgtagacctt gctgcgcggc agcgaggcgg tgagcgccgg gttgagcgcg tggatgccct
     1081 ccaccaccac cacgccgccg ttcagatcca ggtccagcca ttcctccttg cgccgctcgg
     1141 acagaaagtc gaagtccggg atgcgggtgc ggccggtctc cagcagctcg ctcaggcagc
     1201 ggttgaccag cggcacgtcg atggcctgca cgttttcata gtccttgctg ccgtccggca
     1261 ggcgggggta atcctccagg tttttgaaaa agttgtccag cgagatcacg caggaatagc
     1321 ttccgttttt gcagcagaat gcgcccggcc gggccgggcg gcaagacatc ggataacatc
     1381 tctattatac cccgcgtgca ggcgggattg caatcttggg cggctgcggg ggcgaaaatc
     1441 aaaaaaaata aaaaattact cttgcataaa tcgagatcat gtcttataat ataaaagcgc
     1501 cctcggagag ggcagtcaaa aatgggcccg tagctcagct gggagagcgt acggttcgca
     1561 tccgtaaggt cgagagttcg atcctcttcg ggtccaccaa aagcaccgca cagaatagcc
     1621 gaaacggctt gcctgtgcgg tgttttgttt taaaaagatt tggatcagcc cacacaaaag
     1681 cgcgaaaaac cgaaaaaata gggcttgatg tggattgacc ctcgccgggt tgagcgtccg
     1741 catgggagag caagcggttt ttgtcattta tctgaaaaat gcaacgatac gggccttgcc
     1801 ccataaaatt gccgggccat agggattcaa gagtaaaggc gcttcccctt gcagcaccgt
     1861 gggcgcgcct cgccatccgg gtgaagcgcg gctggccact gtttgcagat gcggcgtttt
     1921 ctgatcgcgc cgatcactgc tctgccgcag gttcggaggc ggcgtcagag gcggggggag
     1981 cgggaacggc agaagccaca ccgcccgggg tgggggtggc cacgcccccg gcggcggccg
     2041 aagccggagg cgaacagctc tcttgggcgt cacaggcgtg agcgggtgcg gcggtggagg
     2101 cgctttcttg tttttggggc ggcgaggacg gcgcttgctc ttttttttgc gcgtcttttg
     2161 ccggagcggg gcgggggggc gttttggagg cggcgagcgg caccgtttca tctttccctc
     2221 tcaacaagcg caaaaggaaa ggctctctgt agggagtaaa ggcgtcggaa aaattgtcgg
     2281 ac
//
LOCUS       DBMDYA010000493         2277 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9977_length_2277_cov_1.210478, whole genome shotgun sequence.
ACCESSION   DBMDYA010000493 DBMDYA010000000
VERSION     DBMDYA010000493.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_2277_cov_1.210478"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(193..987)
                     /locus_tag="ACZPFK_11025"
     CDS             complement(193..987)
                     /locus_tag="ACZPFK_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013625.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR1406888.1"
                     /translation="MANTPNAAISPVYPGHVLQSGSSGSEVARVQKYLDALRVKYPTL
                     TTLTVDGRYGSGTSSTVKQYQAIARLSIDGKVGRDTWNALIADYNATIGGSADTYPGL
                     TLRPGMRSEDVRHMQLRLNEIAQKYTGINTQTVDGAYGSNMSDAVRRFQRQFGLSADG
                     ILGAKTWNKIIEVHSRMTSGGPEHVVTPYPGVVLRTGSSGDSVRFVQSYLNAIQGRPI
                     LTVDGRYGQNTARLVGSFQASKGLKVDGKVGRDTWAALIPAFNATL"
     gene            complement(1082..1987)
                     /locus_tag="ACZPFK_11030"
     CDS             complement(1082..1987)
                     /locus_tag="ACZPFK_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406889.1"
                     /translation="MKRSIRPMRFNPGQEMQSREIELRYCLDADSPTVEFHAHPFFEV
                     YLFCEGDLQSYVVGSRSYQLKRGDILLIPPAVMHHPIFRQNAHQYKRYVLWLSDDFLK
                     NIVALDPDMDYAMRLCQEREEYMVRCSTPSLAQTLENYLQSIWFEMRGEGLCRTACAH
                     YACINFLVQLNRTISDKNIVSGVHGHKSPLLDKLITYIHENYASPITLQQTAERFFVS
                     PSTVEHLFSQKLGKSFYRYVIECRIITAQSLILSGVPLKTVSRSCGYPDYSNFYKAFT
                     REVGVSPSEFRTFAPPNHFHIPPLD"
BASE COUNT          468 a          620 c          754 g          435 t
ORIGIN      
        1 ccgccgcgct cctgcggacg tccgcgcccg gccggccccc cccccgcggc cgccactgct
       61 ggaacgcccg gcgcgacggc gggggaaaaa agagataaaa aagggacggc cggctggatt
      121 gccggccgtc tttctttgat tttcaggtcg attttcaggg cccgcctcat ggcggggcgg
      181 gtcaacagag cgctacaggg tggcgttgaa cgcggggatc agcgccgccc aggtgtcgcg
      241 gcccaccttg ccgtccacct tcaggccctt gctggcctgg aaagaaccca ccagccgggc
      301 ggtgttctgg ccgtatctgc cgtccaccgt caggatgggc cggccctgaa tggcgttgag
      361 gtagctctgg acaaaacgga cagaatcgcc gcttgagccg gtgcgcagca cgacgccggg
      421 atagggggtg acgacgtgct cggggccgcc ggatgtcatg cggctgtgca cctcgatgat
      481 cttgttccag gttttggcgc cgaggatgcc gtctgccgag aggccgaact gccgctggaa
      541 gcggcgcacc gcgtcggaca tgttcgagcc gtaggccccg tccaccgtct gggtgttgat
      601 gccggtgtat ttctgggcga tctcgttgag ccgcagctgc atgtggcgca cgtcctcgct
      661 gcgcatgccc gggcgcaggg tgaggccggg ataggtgtcc gcgctgccgc cgatggtggc
      721 gttgtagtcg gcgatcagcg cgttccaggt gtcccgcccc accttgccgt cgatggacag
      781 cctggcgatg gcctggtact gcttgacggt gctggaggtg ccggagccgt acctgccgtc
      841 caccgtgagg gtggtgaggg tggggtattt gacccgcagg gcgtccagat acttctgcac
      901 ccgggccacc tcgctgccgg aactgccgct ctgcagcaca tgccccggat agacggggct
      961 gatggccgcg ttgggggtat tagccataaa tatgatctcc tttccggccc gggcggggcg
     1021 ggaaagggcg cgcccccgcc tggcctatcc ccaggatatg cgcctggaga ggaaagggtg
     1081 ttcagtccag agggggaatg tgaaagtggt tgggcggcgc aaaggtgcgg aactcgctgg
     1141 gcgagacgcc cacctcgcgg gtgaacgcct tgtaaaaatt gctgtaatcc gggtagccgc
     1201 agctgcggct gaccgttttc agcggcacgc cgctgaggat gagggactgg gcggtgatga
     1261 tgcggcactc gatgacatag cggtaaaagg atttgcccag cttttggctg aagaggtgct
     1321 ccaccgtgga cgggctgaca aaaaagcgct cggccgtctg ctgcagggtg atgggggagg
     1381 cgtagttttc atggatatag gtgatgagct tgtccagcag ggggctcttg tgcccgtgga
     1441 cgccggagac aatgtttttg tccgagatgg tgcggttgag ctgcaccaga aaattgatgc
     1501 aggcgtagtg ggcgcaggcg gtcctgcaca gcccctcgcc gcgcatctcg aaccagatgc
     1561 tctgcagata attttccagc gtctgcgcca gcgagggggt ggaacagcgc accatatact
     1621 cctcccgctc ctggcacagc cgcatggcgt agtccatatc cgggtccagc gcgacgatgt
     1681 ttttgagaaa gtcgtcggac agccacagca catagcgctt gtactgatgg gcgttctgcc
     1741 gaaagatggg gtggtgcatc acagcgggcg ggatgagcag gatatccccc cttttgagct
     1801 gatagctgcg cgagcccacc acataactct gcaggtcgcc ctcgcagaac aggtacactt
     1861 caaaaaaagg atgggcgtgg aactccacgg tgggagagtc ggcgtcaaga caatagcgca
     1921 gctcgatctc gcgggactgc atctcctgcc cgggattgaa gcgcatgggg cggatggaac
     1981 gtttcatggc ggacctccgg atctgcggct gcagtatccc aaaattgaca tatacaaaat
     2041 atataaaaaa aacaaaagat gattatggaa tctgtggtta tcatacgaca aacttgcaat
     2101 aaatgcaata taattcgata aaaaagcaat cgtaattgca aatttttcat gctaagatag
     2161 tccacaggaa gaaagtcgca ggggcagcag gatggaagcc cggcggctga taaaagagaa
     2221 atcttcaaaa gagaaaggtg aaaaacaatg aagaacatga aaaagcttct tgccgct
//
LOCUS       DBMDYA010000494         2276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_9984_length_2276_cov_1.377931, whole genome shotgun sequence.
ACCESSION   DBMDYA010000494 DBMDYA010000000
VERSION     DBMDYA010000494.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9984_length_2276_cov_1.377931"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="ACZPFK_11035"
     CDS             complement(<1..221)
                     /locus_tag="ACZPFK_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015084.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1406890.1"
                     /translation="MSLCYLDEVDSTNNWLRRELQGLQDGAAVYTGSQTAGKGRRGRS
                     WQNRPGEALYYSRLVRRPLKDPATLPLMVS"
     gene            complement(218..709)
                     /locus_tag="ACZPFK_11040"
     CDS             complement(218..709)
                     /locus_tag="ACZPFK_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1406891.1"
                     /translation="MGRPTKLIVVSDSHGNRDGLRAVLNAHRDADALIFLGDGLNDLT
                     AVRRSGPCPLVYEVRGNCDYDRTVPSERLVSLGGLLIFMTHGNGYEVKVTTTPLRKAA
                     ARRGADVVLFGHTHSPYYAYIDGVNLFNPGSISVPRVGRPTYGLLLIENGEPEFLHRE
                     VPR"
     gene            complement(712..1572)
                     /gene="rsmI"
                     /locus_tag="ACZPFK_11045"
     CDS             complement(712..1572)
                     /gene="rsmI"
                     /locus_tag="ACZPFK_11045"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1406892.1"
                     /translation="MSERENGCGTLYVVATPIGNLGDLTPRAAETFAQVDFIAAEDTR
                     VTLKLLNHLGLKKPMVSYYEHNLRERGEIILRRLQQGESCALCSDAGMPAVSDPGEVL
                     VTDALARGIPVVPVPAASAAVTALAVSGQPTGRFCFEGFLPMNRRQRRERLAETKDER
                     RTLIFYEAPHKLTSTLADLLEAYGDRSVTVCRELTKLHEQIWKTSLAQALHFYEENAP
                     RGEFVLVVAGAPQPQPEAWTLQQAAALARSLAAGGASATAACKEAAAKSGFAKSEIYR
                     LLTAETEKEG"
     gene            complement(1569..>2276)
                     /locus_tag="ACZPFK_11050"
     CDS             complement(1569..>2276)
                     /locus_tag="ACZPFK_11050"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1406893.1"
                     /translation="DRHRFGTDAMLLSDFCGVHRDWAACDLGSGCGIIPLRWHDAGHR
                     GPCWGVELDPQGTALLQRATRECGAAHITALSADLRALPPALPRAGLELVSCNPPYFT
                     GGFVSQKPGRGAARHEVSCTVEDVCAAAAQLLKDGGRLCLCNRPARLTDCMVAMRLQG
                     IEPKRLRFVRQRPGKGPWLFLLDGRRHGKPGLVLMEDLVIEAAGGGFSAELLRIYGDP
                     GREDGPGPTPKKEEGGL"
BASE COUNT          346 a          814 c          747 g          369 t
ORIGIN      
        1 gagaccatca ggggcagggt ggcggggtct ttcaggggcc gccgcaccag ccggctgtaa
       61 taaagggcct cgccgggccg gttctgccag ctgcgtcctc gtcgcccctt gcctgcggtc
      121 tggctgcccg tatagacggc cgctccgtcc tgcaacccct gcagctcgcg ccgcagccag
      181 ttgttggtgg aatccacctc gtccagataa cacaggctca tcgcggcacc tcccggtgca
      241 aaaattccgg ctcgccgttc tcgatcaaaa gcagcccgta ggtgggcctg cccacccgcg
      301 gcacgctgat ggagcccggg ttgaacaggt tgacgccgtc gatataggcg tagtaggggc
      361 tgtgggtatg gccgaacagc accacgtccg ccccgcgccg ggcagcggcc ttgcgcagcg
      421 gggtagtggt caccttgact tcatagccgt tgccgtgggt cataaagatc agcagcccac
      481 ccaggctcac cagccgctca gaggggacgg tgcggtcgta atcgcagttg ccccgcacct
      541 catagaccag ggggcagggg ccgctgcgcc gcacggccgt caggtcgttc aggccgtcgc
      601 cgagaaagat cagcgcgtcg gcgtcccggt gggcgttcag caccgcccgc agcccgtccc
      661 ggttgccgtg ggagtcggat acgacgatca atttggtagg cctgcccaac cctatccctc
      721 cttttccgtc tctgccgtca gcaggcggta gatctcgctt ttcgcaaagc cgcttttggc
      781 ggcggcctcc ttgcaggcgg ccgtggcgga cgccccgcct gcggcgaggc tgcgggccag
      841 cgcggcggcc tgctgcagcg tccaggcctc gggctggggc tgcggcgcgc ccgccaccac
      901 cagcacgaac tcgcccctcg gggcgttttc ctcataaaaa tgcagcgcct gggcgaggct
      961 ggtcttccat atctgctcgt ggagcttggt cagctcacgg cagacggtga cgctgcggtc
     1021 gccgtaggcc tccagcagat cggccagggt ggaggtgagc ttgtgggggg cctcgtaaaa
     1081 gatcagggtg cggcgctcgt ccttggtctc ggccaggcgc tcgcgccgct ggcggcggtt
     1141 catgggcaaa aagccctcaa agcaaaaccg cccggtgggc tgccccgaca ctgccagcgc
     1201 ggtcaccgcg gcgctggcgg ccggcaccgg caccaccggg atgccccttg ccagcgcgtc
     1261 ggtgaccagc acctcgcccg ggtcgctgac tgcgggcatc cccgcgtcgc tgcacagggc
     1321 acagctctcg ccctgctgca gccgccgcag aatgatctcg ccccgctcgc gcaggttgtg
     1381 ttcataatag ctgaccatcg gttttttgag ccccagatgg ttgagcagct tgagggtgac
     1441 gcgggtgtcc tcggcggcga tgaaatccac ctgtgcaaag gtctcggccg cccgcggggt
     1501 cagatccccc aggttgccga tgggggtcgc caccacatat aaagtgccgc atccgttttc
     1561 tctctcgctc aaaggccgcc ctcctctttt ttcggcgtcg gtccgggacc gtcctcccgc
     1621 cccgggtcgc cgtagatgcg cagcagctcg gcggaaaaac cgccccccgc cgcttcgatc
     1681 accaggtcct ccatcagaac cagccccggt ttgccgtgtc gcctgccgtc caacagaaaa
     1741 agccagggcc ctttgcccgg ccgctggcgc acaaagcgca gccgcttggg ctcgatgccc
     1801 tgcaggcgca tggccaccat gcagtcggtc agccgcgccg gccggttgca caggcaaagg
     1861 cggccgccgt ccttcagcag ctgcgccgcg gccgcgcaga catcctccac cgtacagctc
     1921 acctcatgcc gggccgcgcc cctgcccggc ttttgggaca caaagccgcc ggtaaaatag
     1981 ggtgggttac agctcaccag ctccagcccc gcccgcggca gggcgggggg cagagcccgc
     2041 aaatccgccg agagcgccgt gatgtgcgcc gcgccgcact cgcgcgtcgc gcgctgcaac
     2101 agcgccgtgc cctgcgggtc cagctcgacc ccccagcagg ggccgcgatg gcccgcgtcg
     2161 tgccagcgca gcgggatgat gccgcagccg ctgcccaggt cgcaggcagc ccaatcccgg
     2221 tggacgccgc agaagtcgga cagcagcatg gcgtcggtgc cgaaccggtg cctgtc
//
LOCUS       DBMDYA010000495         2272 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10006_length_2272_cov_1.285583, whole genome shotgun sequence.
ACCESSION   DBMDYA010000495 DBMDYA010000000
VERSION     DBMDYA010000495.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10006_length_2272_cov_1.285583"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="ACZPFK_11055"
     CDS             complement(<1..1013)
                     /locus_tag="ACZPFK_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406894.1"
                     /translation="MSKLNRFESLHLYIQSISLYRETAESPALRFTRQMAALYDPKSW
                     PGKDLVLEMAYTGEGEEEVFQLRLGRDGAEVCADQAAFAPYTARVETPLRLWRQIGAG
                     EVDAMQALAEQRYHILGDLRFMSRWDDFFSAGAPARAAGQNGAGPATPVRDGRRSPSF
                     SLLLLPWIVFSCAQPLGTAVGAAVTLAACLLLAPASLRWQLTPFDAAEGVLLGAYAAV
                     APLLGPGWEPRLRALATLGYALLWLGTGLFARVPLTAWYSSHDFGGRRAWGNPLFLRT
                     NRILTLVWGVYFLFSAALPLWALGAGAAPLAIALAGQLAPLPLTAFTFWFQRWYPASA
                     ARGR"
     assembly_gap    1084..1093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1094..1810)
                     /locus_tag="ACZPFK_11060"
     CDS             complement(<1094..1810)
                     /locus_tag="ACZPFK_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1406895.1"
                     /translation="MKVLLLNGSPRGEGSGTLKLARAFLEGLQDACGAEVETVHLIEK
                     KILPCRGCFGCWTGTPGRCVQRDDMDGLLPRYRQAELVVWCLPLYYFGMPSHAKAFLD
                     RLLPSNLPRMEPRPQGGMGHPPRWPETRRQRHLLISTCGFCARQNNYEALEKQFEIAF
                     GGEEGRLTQILCPEGELFRVPQLRGRTGAYLALLREAGREFGRAGRIAPGTRAALEKL
                     LYPPEAFLKMADASWEVAGDP"
     gene            complement(1807..>2272)
                     /locus_tag="ACZPFK_11065"
     CDS             complement(1807..>2272)
                     /locus_tag="ACZPFK_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406896.1"
                     /translation="LAAAGRGAGIAFKPGDAVTTCFVSFTVLVLCYAALDLAGWVWPL
                     DSTQALRLFALTAAIGVLMQLTALLPVRRLAWQLLLQFADVAAVVFGLGGLMGAFPWE
                     GPVLLLVGGMLTFTYFASLLVFRMIEFSCNRRDSLRINRMLARTQAAKGESR"
BASE COUNT          404 a          834 c          731 g          293 t
ORIGIN      
        1 cggcctcggg ctgcggatgc cggataccag cgctgaaacc aaaaggtgaa cgccgtcagc
       61 ggcagcggcg ccaactgccc ggccagcgcg attgccagcg gggccgcccc ggcccccagc
      121 gcccacagcg gcagcgcggc gctgaacaaa aagtagacgc cccacaccag cgttaaaatg
      181 cggttggtgc gcagaaacag cgggttgccc caggcgcgcc ggccgccaaa atcgtggctg
      241 gaataccagg ccgtcagcgg cacccgggca aagaggcccg tgcccagcca gagcagggcg
      301 taccccagcg tcgccagggc ccgcagccgc ggctcccacc cggggcccag cagtggggcc
      361 acagcggcat aagcccccag cagcaccccc tcggccgcat cgaacggggt cagctgccag
      421 cgcagcgagg ccggcgccaa aagcaggcag gcggccagcg tcaccgccgc ccccaccgcg
      481 gtgcccaggg gctgcgcgca ggaaaagacg atccagggca acagcagcag cgagaagctg
      541 ggcgaccgcc gcccgtccct gaccggcgtc gccgggccgg ccccgttttg gcccgcggcg
      601 cgcgccggtg caccggcgct gaaaaagtcg tcccagcggc tcataaagcg caggtcgccc
      661 aggatgtgat accgctgctc cgccagcgcc tgcatggcgt cgacctcccc ggcgccgatc
      721 tgtcgccaca gccgcagcgg ggtctccacc cgcgctgtgt agggcgcgaa cgccgcctgg
      781 tccgcgcaga cctccgcgcc gtcccggccc aaccgcagct gaaaaacctc ctcctccccc
      841 tccccggtat aggccatctc cagcaccaaa tccttgcccg gccagctttt ggggtcatag
      901 agggccgcca tctgccgggt gaagcgcagg gccgggctct cggcggtctc ccggtagagg
      961 gagatggact ggatgtagag gtggagggat tcaaagcggt tgagtttgct catgtcggcg
     1021 gcttcctttt cgtctttatt ttcttttatt ctaccgggtt tgccccccac cgtcaaccaa
     1081 aggnnnnnnn nnncgggtcc ccggccacct cccagctggc gtcggccatt tttaaaaagg
     1141 cctccggcgg gtagagcagc ttttccagcg cggcgcgggt gccgggggcg atgcggcccg
     1201 cgcggccgaa ctcccttccc gcctcccgca gcagcgccag ataggcgccg gtgcgcccgc
     1261 gcagctgcgg cacccggaac aactccccct ccgggcagag gatctgggtc aggcggccct
     1321 cttccccgcc gaaagcgatc tcaaactgct tttccagcgc ctcatagttg ttctgccgcg
     1381 cgcagaaccc gcaggtggag atcagcagat ggcgctggcg ccgcgtctcc ggccagcggg
     1441 gcgggtgccc catgccgccc tgcggccggg gctccatgcg gggcaggttc gagggcagca
     1501 gccggtcgag aaaggccttg gcgtgggagg gcatgccgaa atagtaaagg ggcaggcacc
     1561 agaccaccag ctccgcctgc cggtaacggg gcagcagccc gtccatatcg tcccgctgca
     1621 cacagcggcc cggcgtgccc gtccagcagc cgaagcagcc gcggcagggc agaatctttt
     1681 tttcgatcag atggaccgtc tccacctcgg cgccgcaggc atcctgcagc ccctctaaaa
     1741 aggcgcgggc cagcttcagc gtgccgctgc cctcgccgcg cgggctgccg ttgagcagaa
     1801 gcactttcat cggctctccc cctttgccgc ctgcgtgcgg gccagcatgc ggttgatgcg
     1861 cagggaatcg cgtcggttac aggagaactc gatcatgcga aaaaccagca gcgaggcaaa
     1921 ataggtaaag gtcagcatgc cgcccaccag cagcagcacc ggtccctccc agggaaaggc
     1981 ccccatcagg ccccccaggc caaagaccac cgccgccacg tccgcgaact ggagcagcag
     2041 ctgccaggcc agccgccgca ccggcagcag cgcggtgagc tgcatcaaca ccccgatggc
     2101 cgccgtgagc gcgaacagac gcagcgcctg ggtgctgtcc agcggccaca cccagcccgc
     2161 caggtccagt gccgcatagc agagcaccag cacggtaaag gacacaaagc aggtggtcac
     2221 cgcgtccccc ggcttgaacg cgatgccggc gccccgcccc gccgcggcca ag
//
LOCUS       DBMDYA010000496         2261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10065_length_2261_cov_1.542130, whole genome shotgun sequence.
ACCESSION   DBMDYA010000496 DBMDYA010000000
VERSION     DBMDYA010000496.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10065_length_2261_cov_1.542130"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="ACZPFK_11070"
     CDS             complement(<1..410)
                     /locus_tag="ACZPFK_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HIR1406897.1"
                     /translation="MLQKNEVMTVSDLSKALNVSLSTARRDVNLMLVEGSVERLRGGA
                     FRISRASPVIPPEMQPTAPQADHSDTDAKTRIAQKAARLVRDNEIIFVDSGSTLRRIP
                     QFITAQNVTLVVRSLAVLETVPQNGSISVILLGG"
     gene            782..1792
                     /locus_tag="ACZPFK_11075"
     CDS             782..1792
                     /locus_tag="ACZPFK_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406898.1"
                     /translation="MTQYFSMPTAVVLTLLAAAMWGSWMQVVKLKKDFPIAGIVFWLY
                     LFSFVFVWAITLVLAPSLLPQGIWAVTMQNLPVIGKILLGGAMMSVGMLLSLTVMHSI
                     GLMLATTLSGTITSILGIFTSVSEEGLPDNPFALPLIIATTVVFLLASFVCSYASQQC
                     GKDRLLAQGGRGTAVKQKSTVTFKVVLMILLNSVLANGWSIGTASGTAAGLPPILTCA
                     YLCTGSTISTLLVCGFLFTRQKCWKTIFCVGTSKRPLLLGVVSGLCHYGGNLMSIYSM
                     PTISATLSFLFGRTANLWTYFWGFYYKEFQGARKKTLAVLSLGLLLYFGGLGLLFLYN
                     FS"
     gene            1949..>2261
                     /locus_tag="ACZPFK_11080"
     CDS             1949..>2261
                     /locus_tag="ACZPFK_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1406899.1"
                     /translation="MKYNALGKTGLLVSEVGLGCEFLQGKSEAVVRAVLDAALAKGVN
                     IIDVFMSEPEVRTNIGKALVGRRDQVYIQGHLCAVWKNGQYGRSRDPEEVRFFFEDLL
                     TR"
BASE COUNT          421 a          632 c          692 g          516 t
ORIGIN      
        1 tccccgccca gcaggatgac ggagatggag ccgttttgcg gcacggtctc cagcaccgcc
       61 aggctgcgca ccaccagagt cacgttttga gcggtgatga actgggggat cctgcgcagc
      121 gtgctgccgg aatccacaaa aatgatctcg ttgtcccgca ccagccgcgc cgctttttgg
      181 gcgatcctgg tcttggcgtc ggtgtcgctg tggtccgcct gcggcgctgt gggctgcatt
      241 tccggcggga tgaccggcga ggcgcggctg atgcggaacg cccctccgcg cagccgctcg
      301 accgagccct ccaccagcat caggttgaca tccctgcgcg cggtggagag ggagacgttc
      361 aacgctttgg agagatcgga caccgtcatc acttcatttt tttgcagcaa agagaggatg
      421 tctttgcgtc ggaaataagg agccagcata taatacccca tttttgacga atcgagcagt
      481 cgccattttt cttgacatta ttataacaac agcctcctca atttacaatc tttatctctt
      541 tcactatccc tccgcccccg ccgcccgccc gggccgggcg ccgcggacgg gcagcagctt
      601 ttatcctctt ttcctctctg taccacccgg ggaaggataa aatttgattg gattttttgt
      661 gaaataaaaa gtaaactttg aattgatttt atcgaaaaat ggagttaaaa tgaatttgtt
      721 tggaaattat tttgaacttt ttgaaaagag aagaaatgcg gaataaaagg agttgttgtt
      781 ggtgacccaa tatttttcca tgccgacggc ggtggtgctg accctgctgg cggcggcgat
      841 gtggggcagc tggatgcagg tggtaaagct gaaaaaggac tttcccatcg cgggcatcgt
      901 gttctggctc tatttgttct cgttcgtgtt cgtctgggcc atcaccctgg tgctcgcccc
      961 ctcgctgctg ccgcagggga tctgggcggt gacgatgcag aatctgccgg tgatcggcaa
     1021 gatcctgctg gggggcgcca tgatgtcggt ggggatgctt ttgagcctga ccgtcatgca
     1081 cagtatcggg ctgatgctgg ccaccaccct gagcggcacc atcaccagca tcctgggcat
     1141 cttcacctcc gtctcggagg agggactgcc cgacaatccc tttgccctgc cgctcatcat
     1201 cgccaccacc gtggtgtttt tgctggcgag ctttgtctgc tcgtacgcct cgcagcagtg
     1261 cggaaaagac cggctgctgg cgcagggggg gcgggggacg gccgtgaagc agaagagcac
     1321 ggtcactttc aaggtcgtgc tgatgatttt gctcaattcg gtgctggcca acggctggtc
     1381 gatcggcacc gcgtccggca ccgcggccgg gcttcccccc attttgacct gtgcctacct
     1441 gtgcacgggc agcacgatca gcacgctgtt ggtctgcggt ttcctcttca cccggcaaaa
     1501 atgctggaag accatcttct gcgtcggcac cagcaagcgg ccgctgctgc tgggcgtggt
     1561 ctcgggcctg tgccactacg gcggcaacct gatgtccatc tactccatgc ccaccatcag
     1621 cgccaccctg tctttcttgt tcgggcgcac ggcgaacctg tggacctatt tctggggctt
     1681 ttattacaag gagttccagg gcgcgcgaaa aaagaccctt gcagtgctga gcctgggctt
     1741 gctgctctat ttcggcggcc tggggctgtt gttcctgtat aatttcagct gacccggccc
     1801 tcttggggcg ccggaaaata cggaagaaaa gagaccatcg tttggagcac agcccgcacc
     1861 gggcaggccg cccggcgctg gtctctttgt tgtttttcag ttctcttgac agggccggcg
     1921 gacggcgccg gcagaaaggg tgaaaaaaat gaagtacaac gctttgggaa agaccggttt
     1981 actggtgagc gaggtggggc tcggctgcga attcctccag gggaaaagcg aggcggtggt
     2041 gcgcgcggtg ctggacgccg cgctggcgaa aggggtcaac atcatcgacg tgttcatgtc
     2101 cgagccggag gtgcgcacca acatcggcaa ggcgctggtc ggccggcggg accaggtgta
     2161 tatccagggc catctgtgcg cggtgtggaa gaacggccag tatggccgca gccgcgaccc
     2221 cgaggaggtg cgctttttct ttgaggatct tttgaccagg c
//
LOCUS       DBMDYA010000497         2259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10084_length_2259_cov_1.484708, whole genome shotgun sequence.
ACCESSION   DBMDYA010000497 DBMDYA010000000
VERSION     DBMDYA010000497.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_2259_cov_1.484708"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="ACZPFK_11085"
     CDS             <1..1825
                     /locus_tag="ACZPFK_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1406900.1"
                     /translation="AAIPEGLPAIVTIVLALSVGRILKKGAVIRKLHAVETLGSAGVI
                     CTDKTGTLTQNKMTVKELYCGGRGISVGGDGYAAQGSFSCAGKYVDAARDPLLGRLFT
                     VMALCTEARATLKKDRYEVQGDPTETALLVAAGKAGVTREKLLAVWKLTGERPFDSAR
                     KMMSVTARGRGGELLLCKGAPDVLLGHCSRVALPGGSRAMTAADRREIENAAEAMAKR
                     ALRVIGLAESPNPGAGEKDLVFLGLAGMIDPPRPEVAPAVRQCRRAGIKPVMITGDHK
                     DTAAAIAREVGILRPGDEVLTGAEIEKLTDAELAARCRRVSVYARVSPAHKLRIVKAW
                     KAAGKVVAMTGDGVNDAPAVKAADIGVAMGISGTDVTKEAASVVILDDNFATIVSSVE
                     EGRVIYQNIRSFIRYLLTCNLGEVLSMLFSMMLGAPVALLPIQILMVNLLTDGLPAIA
                     LGMEPPSHKIMEKPPRPKNEGLFAHGLMATIIVRGIFLGACTSGSFYAVYQMSGGDIQ
                     LARSAGYLVLVLSQMVHIFECRGKGFSLIGNPYLIFASLVSVSSTLLTVYLPVLQPIF
                     RTTAVGPEYALPVLAGIFAGPVLFFVLRKVWHLLRRGRRAS"
     gene            complement(1842..>2259)
                     /locus_tag="ACZPFK_11090"
     CDS             complement(1842..>2259)
                     /locus_tag="ACZPFK_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1406901.1"
                     /translation="GLLHLIADAIAESGFPAIVGPSLASRSKIEVEQSDFHTPGVLHK
                     AGVLIALTTDHPVTRIQYLPLCAALAAKEGLGEEAALRAITIDAARICRVDGRLDSLR
                     VGKDADLAIFDGSPLVIASRVRATVVGGRIVYRPEG"
BASE COUNT          361 a          739 c          799 g          360 t
ORIGIN      
        1 ggccgccatc ccggagggcc tgcccgccat cgtcaccatc gtgctggccc tctcggtggg
       61 gcgcatcctg aaaaaaggcg ccgtcatccg caagctgcac gcggtggaaa cgctgggcag
      121 cgccggggtc atctgcaccg acaagaccgg caccctgacc cagaacaaga tgacggtgaa
      181 agagctctat tgcggtgggc ggggcatttc ggtggggggc gacggctacg cggcgcaggg
      241 cagcttttcc tgcgcgggca agtatgtgga cgccgccagg gacccgctgc tgggccgcct
      301 tttcaccgtg atggccctgt gcacggaggc gcgggccacc ctgaaaaaag accgctatga
      361 ggtacaggga gaccccaccg agaccgccct gctggtggcg gcgggcaagg cgggggtcac
      421 aagagaaaaa ctgctggccg tgtggaagct caccggcgag cgcccgtttg actcggcgcg
      481 caagatgatg agcgtcacgg cccggggccg cggcggcgag ctgctgctgt gcaagggcgc
      541 gcccgacgtg ctgctggggc actgcagcag ggtggcgctg cccggcggca gccgggccat
      601 gacggcggcg gaccggcggg agatcgagaa cgccgccgag gcgatggcaa agcgggccct
      661 gcgggtgatc ggcctggccg agagccccaa ccccggcgcg ggggaaaaag acctggtctt
      721 tctcggcctc gcgggcatga tcgatccgcc ccggcccgag gtggcgcccg cggtgcggca
      781 gtgccgccgc gcaggcatca agccggtgat gatcaccggc gaccacaagg acacggccgc
      841 cgccattgcg cgggaggtgg gcatcctgcg gcccggggac gaggtgctca ccggtgcgga
      901 gatcgaaaag ctgaccgacg cggagctggc ggccaggtgc cgccgggtgt cggtctacgc
      961 gcgggtgagc ccggcccaca agctgcgcat cgtcaaggcc tggaaagcgg ccggcaaggt
     1021 ggtggccatg accggggacg gcgtgaacga cgccccggcg gtcaaggcgg cggatatcgg
     1081 ggtcgccatg ggcatctccg gcaccgacgt caccaaggag gccgccagcg tggtcatcct
     1141 ggacgacaat ttcgccacca tcgtctcgtc ggtggaggag ggccgggtca tctaccagaa
     1201 catccgcagc tttatccgct acctgctgac ctgcaatctg ggcgaggtgc tcagcatgct
     1261 cttttcgatg atgctgggcg cgccggtggc gctgctgccc atccagatcc tgatggtcaa
     1321 cctgctcacc gacggcctgc cggccatcgc gcttggcatg gagcccccct cccacaaaat
     1381 catggaaaag ccgcccagac cgaagaacga gggccttttt gcccacggtc tgatggccac
     1441 catcatcgtg cgcggcatct ttctcggcgc ctgcacctcc ggctcttttt acgcggtcta
     1501 ccagatgagc ggcggggata tccagctggc ccgcagcgcc gggtatctgg tgctggtgct
     1561 ctcccagatg gtgcacatct tcgagtgccg aggcaaggga ttttcactta tcggcaaccc
     1621 ctatctcatc ttcgcctcgc tcgtttcggt gtcctccacc ctgctcaccg tctatctgcc
     1681 ggtgctgcag cccatcttcc gcaccactgc ggtggggccg gagtacgcgc tgccggtgct
     1741 ggcgggcatc tttgcggggc cggtgctctt ttttgtgctg cgcaaggtct ggcacctgct
     1801 ccgccgcggc cgccgcgcct cctaagggtg aggcggggcg ttcagccctc gggccggtag
     1861 acgatgcggc cgcccacgac ggtggcgcgc acacggctgg cgatcaccag ggggctgccg
     1921 tcaaagatgg cgagatccgc gtccttgccc acccgcaggc tgtccagcct gccgtccacc
     1981 cggcagatgc gggcggcgtc gatggtgatg gcgcgcagcg cggcctcctc gcccaatccc
     2041 tcttttgccg ccagggcggc gcagagcggc aggtattgga tgcgggtgac cgggtggtcg
     2101 gtggtcaggg cgatcaggac accggccttg tgcaggaccc cgggggtgtg aaagtcggat
     2161 tgctccacct cgatcttgct gcgcgaggcc agggacggcc ccacaatagc agggaaaccg
     2221 ctctcggcaa tggcgtcggc gatgagatgg agcaatccg
//
LOCUS       DBMDYA010000498         2235 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10202_length_2235_cov_1.254452, whole genome shotgun sequence.
ACCESSION   DBMDYA010000498 DBMDYA010000000
VERSION     DBMDYA010000498.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10202_length_2235_cov_1.254452"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="ACZPFK_11095"
     CDS             complement(<1..1473)
                     /locus_tag="ACZPFK_11095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR1406902.1"
                     /translation="MKLNSDKTWNLADMFPDDAAWERQMEEVRALGAQLAARKGHAAD
                     SAAALLDTATLYDSLNCKGAQCAVYAECRFHTDMASPQGKEMVGKLEVLFTALGEQTA
                     FLAPELMQYSYADFERYCAACPQLRVYDQLARDFFDRKEHVLDDVGEAMLTRMADLGG
                     TYRQVFEDLVINDTPSPEVEGPDGKPVKVTEAGYGAALQNPDRPFRARYYNALLGLYG
                     GHINTLSSSYYGRVKADAYLAKSRRYPSARAAALSGNFIPENVYDNLVSTARAHTGPL
                     QEYVSLRKRVLGIEDFHFYDFFVPLAEDVAREYPFEEAAELVLAATAPLGEDYAEKMR
                     RALENRWIDVYPKQNKQTGAYSTGTYGFHPYMLLNYDGTLEDVFTLVHELGHSMHTMY
                     SCENQPFVYSDYSIFCAEVASTANEQLLYHYLLEHCESRELRRLLLAKHLDDLRSTFY
                     RQTMFADFERETHARVEAGEPLLPATLCGIHKKLNEDYYGP"
     gene            1792..2178
                     /locus_tag="ACZPFK_11100"
     CDS             1792..2178
                     /locus_tag="ACZPFK_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1406903.1"
                     /translation="MRIEHVALYTFDLEKLKRFYTAYFGAVGDKPYHNPRTGLCTCFL
                     TFPDGGGRLELMTRPQLQRQERPAFCTGYTHLAFSLGGREAVDALTARLAADGYEVKS
                     GPRVTGDGYYESCVLDPDGNEVELTA"
BASE COUNT          407 a          726 c          688 g          414 t
ORIGIN      
        1 cggcccgtaa tagtcctcgt tcagcttttt gtggataccg cagagggtcg cgggcagcag
       61 cggctccccc gcctccaccc gggcgtgggt ctcgcgctca aaatccgcaa acatggtctg
      121 ccggtaaaag gtggagcgca gatcgtccag gtgctttgcc agcagcaggc ggcgcagctc
      181 gcgcgactcg cagtgctcca gcagatagtg atagagcagc tgctcgttgg ccgtagaggc
      241 cacctcggcg cagaagatgg agtagtcgga gtagacaaag ggctggttct cgcaggagta
      301 catggtgtgc atggagtgcc ccagctcatg caccagcgta aagacatcct ccagcgtgcc
      361 gtcatagttg agcagcatat agggatggaa cccgtaggtg ccggtggaat aggcgccggt
      421 ctgcttgttc tgcttgggat agacgtcgat ccagcggttc tccagcgccc ggcgcatctt
      481 ctcggcatag tcctccccca gcggggcggt ggcggcaagc accagctccg ccgcctcctc
      541 aaacgggtac tcccgcgcca catcctccgc cagggggacg aaaaagtcat agaaatggaa
      601 atcctcgatg cccagcaccc gcttgcgcag cgatacatat tcctgcagcg gcccggtgtg
      661 ggcgcgggcg gtggagacca ggttgtcgta gacattctcc gggataaagt tgcccgagag
      721 cgcggccgcc cgggcgctgg gatagcggcg gctcttggcc agataggcgt cggcttttac
      781 ccggccgtaa tagctggagg acagcgtgtt gatgtgcccg ccgtaaaggc ccagcagggc
      841 gttgtaatac cgggcgcgga acgggcggtc cgggttttgc agcgccgcgc cgtagcccgc
      901 ctcggtgacc ttgaccggct tgccgtccgg tccctccacc tcgggcgagg gggtgtcgtt
      961 gatgaccaga tcctcaaaca cctgccgata ggtgccgccc aggtcggcca tgcgggtgag
     1021 catcgcctcg ccaacgtcgt ccaacacgtg ctccttgcgg tcaaaaaagt cccgcgccag
     1081 ctggtcgtaa acccgcagct ggggacatgc ggcgcaatag cgctcaaaat ccgcatagct
     1141 gtactgcatc agctccggcg cgagaaaagc cgtctgctcg cccagcgctg tgaacaggac
     1201 ctccagcttg cccaccatct ccttgccctg gggcgaggcc atgtcggtgt gaaagcggca
     1261 ctcggcgtaa accgcacact gcgccccctt gcagttcagg ctgtcataga gggtcgcggt
     1321 gtccagcagc gccgcggcgc tgtccgccgc gtgtcccttg cgggctgcga gctgtgcgcc
     1381 cagggcgcgc acctcctcca tctggcgctc ccacgccgca tcgtcgggga acatgtcggc
     1441 caggttccag gttttatcac tgttcagctt caaatccgtc tctccttttc ccggcggcag
     1501 ggctttttac gcccgctcaa agcgccgccg cagcgatcgt tttcacacgt tattgtagca
     1561 cgcggttgtt gcgaaaaaaa gaacaaattt cctgcgcggc gcaaagaggc gcccgccgcc
     1621 ccccgttttg ccccttgctg gattttcgcc tcgccctgtg ctacaatcag gctgtatctc
     1681 agctgttgcc gccgcagggg acgagctttc gtcaaatgca gaatccccct ctctctccgc
     1741 cgccgtcagg ccgcacgggc ggcacgaacc agaaagtgag gttatcccgc catgcggatc
     1801 gaacacgtcg ccctttacac ttttgatctg gaaaagctga aacgctttta caccgcttat
     1861 ttcggcgctg tgggggacaa gccctaccac aacccccgca ccggcctttg cacctgcttt
     1921 ctcacctttc ccgacggcgg cggccggctg gagctgatga cccgccccca gctgcaaagg
     1981 caggagcggc ccgccttttg caccggctat acccatctgg ccttttcgct gggcggcagg
     2041 gaggcggtgg acgccctcac cgcccgtctg gcggcggacg gatatgaagt aaaaagcggc
     2101 ccccgggtca ctggggacgg ctattacgag agctgtgtgc tggacccgga cggcaacgag
     2161 gtggagctga ccgcctgagc ggccgccccc cataccaaaa caggagaacg acgcacgcat
     2221 gagaaagacc atcgg
//
LOCUS       DBMDYA010000499         2230 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10232_length_2230_cov_1.939878, whole genome shotgun sequence.
ACCESSION   DBMDYA010000499 DBMDYA010000000
VERSION     DBMDYA010000499.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_2230_cov_1.939878"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            508..735
                     /gene="acpP"
                     /locus_tag="ACZPFK_11105"
     CDS             508..735
                     /gene="acpP"
                     /locus_tag="ACZPFK_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1406904.1"
                     /translation="MVFEKVREILCDQLDLEEDKVTMDSDIVEDFEADSLDVVDLVMS
                     LEDEFNIEIPDEEIENIKTVGDVVRYIEENT"
     gene            824..>2230
                     /gene="proS"
                     /locus_tag="ACZPFK_11110"
     CDS             824..>2230
                     /gene="proS"
                     /locus_tag="ACZPFK_11110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1406905.1"
                     /translation="MSEQNKKMVEAITPINEDFAQWYTDICKKAELVDYSSVKGCVIL
                     RPYGYAIWENIQHILDGMFKATGHQNVAMPLFIPESLLQKEKDHVEGFAPECAWVTMG
                     GGEQLEERLCVRPTSETLFCDHFAHVLQSYRDLPLLYNQWCSVVRWEKTTRPFLRSRE
                     FWWQEGHTIHETAEEAVAETEQQLNCYAEFCEKYLRIPVIKGRKTESEKFSGAEATYT
                     IEAMMHDGKALQSGTSHYFGDGFSRAFEVTFAGRDNKLQYPFQTSWGMSTRIVGAIIM
                     THGDDEGLILPPAVAPIQVVVVPVAQHKPGVLDKAQELKERLSKEFRVHLDASDNSPG
                     WKFSQYEMKGVPLRLEIGPKDIEKGQCVLVRRDNREKTFVPLDQLEAEVGRLLAELGD
                     NLYRRALENRENRTFSAVTMEEVKQLAEEHTGFIKTMWCGDAACEEKMKEEAGLSSRC
                     MPFEQEHLSDVCPCCGKPA"
BASE COUNT          492 a          648 c          706 g          384 t
ORIGIN      
        1 cgcggccgcg gcgaaagcca atatcgccga ctacaagccg gtgatgagca aggacgagta
       61 catcgcgttt atggagagca tgcagaccac cgagaccgta gagcccaccc cgctgcccat
      121 cgtcggccag aaagagcagt gagcacggcg cggccccgcg cccggctgca gggccgccgc
      181 gcaggagctt gaggcccgtg ggccgctcaa aatctctgtg gccgctttga tcgctttgcc
      241 gtgcaaagct gtcaaaggat gcgacggcaa aagcgaaaag cacaccctga acaaggcgca
      301 gagctctttt cggggtgtgc ttttctcacg ccctttttcg ggcgggacgg tttgcggccc
      361 gcccctttcc gcgccttttg ggggccggta gagggcggcg gcgccgccgg cggcaagatt
      421 ctcaaaatct tttgccgata cgccttgaca aggcatctaa aagacggtat aaaagtgtat
      481 aggaaaattc tctaggagga acgatcaatg gtttttgaaa aagtcaggga gatcctttgc
      541 gatcagctgg atctggaaga ggataaggtc acgatggact ctgatatcgt cgaggacttc
      601 gaggcggatt ccctcgacgt ggtcgacctg gtaatgtccc tcgaggatga gttcaacatt
      661 gagatccccg acgaggagat cgaaaacatc aagactgtcg gcgacgtggt gcgctatatc
      721 gaagaaaata cctgattgtt gaaagcgtag aacaacaaag cctcccaacc gtgaacgtag
      781 gaggctttgt tttttcggat gagaaaaggg agtgtcaagg cgaatgagcg aacagaacaa
      841 aaaaatggtg gaggccatca cccccatcaa cgaggacttt gcgcagtggt acaccgacat
      901 ctgcaaaaag gcggagctgg tggactattc ctcggtcaag ggctgcgtga tcctgcgccc
      961 ctatggctat gccatctggg agaatatcca gcacatcctg gacggcatgt tcaaggccac
     1021 cggccaccag aacgtggcca tgccgctgtt tatccccgag agcctgctgc aaaaggaaaa
     1081 agaccacgtg gagggcttcg cccccgagtg cgcctgggtg accatgggcg gcggcgagca
     1141 gctggaggag cgcctttgcg tgcggcccac cagcgagacc ctgttctgcg accacttcgc
     1201 ccatgtgctg cagtcctacc gcgacctgcc gctgctgtat aaccagtggt gcagcgtggt
     1261 gcggtgggaa aagaccaccc gcccgttcct gcgcagccgc gagttctggt ggcaggaggg
     1321 gcacaccatc cacgagaccg ccgaggaggc ggtggcggag accgagcagc agctcaactg
     1381 ctatgccgag ttctgcgaaa aatacctgcg catcccggtg atcaagggcc gcaagaccga
     1441 gagcgaaaag ttctcgggcg cagaggccac ctacaccatc gaggccatga tgcacgacgg
     1501 caaggcgctg cagagcggca ccagccacta tttcggcgac gggttctccc gtgcttttga
     1561 ggtgaccttt gccggccgcg acaacaagct gcagtacccg ttccagacca gctggggcat
     1621 gtccacccgc atcgtcggcg ccatcatcat gacccatggc gatgacgagg ggctgatttt
     1681 gccccccgcc gtggccccca tccaggtggt tgtcgtgccg gtggcccagc acaagccggg
     1741 ggtcctggac aaggcgcagg agctgaaaga gcgcctgtcc aaggagttcc gggtgcatct
     1801 ggacgcctcg gacaactcgc ccggctggaa gttcagccag tacgagatga aaggcgtgcc
     1861 gctgcgcctg gagatcggcc ccaaggatat cgaaaagggc cagtgcgtgc tggtgcggcg
     1921 ggacaaccgt gaaaagacct ttgtgcccct ggatcagctg gaggccgagg tcggccgcct
     1981 gctggcggag ctgggcgaca acctctaccg gcgcgcgctg gagaaccgtg agaaccgcac
     2041 gttcagcgcc gtcaccatgg aggaggtcaa gcagctggcc gaggagcata ccggctttat
     2101 caagaccatg tggtgcggcg acgcagcctg cgaggagaag atgaaagagg aggccggcct
     2161 ttccagccgc tgcatgcctt tcgagcagga acacctctcc gacgtctgcc cctgctgcgg
     2221 caagcccgcc
//
LOCUS       DBMDYA010000500         2222 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10279_length_2222_cov_1.580387, whole genome shotgun sequence.
ACCESSION   DBMDYA010000500 DBMDYA010000000
VERSION     DBMDYA010000500.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10279_length_2222_cov_1.580387"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="ACZPFK_11115"
     CDS             complement(<1..966)
                     /locus_tag="ACZPFK_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIR1406906.1"
                     /translation="MTAYNRPAVTVDLKNVIGPAFYESHRAIREQRAHTLVEDGGRGS
                     LKSSFCSTEVVLWLLKWPQSHALVMRQMGNTLEDSVYSQMLWAIAKLGLTEHFLQKKS
                     PLRLVYKPTGQTIYFRGLDDEMKIKGIKPKFGYIGCLWFEEADQLRRGENAVLSVKQS
                     AFRGSGDNPTLTLVSFNPPANARNWANRYAREQQPGKLVHHSSYLDAPREWLGKEFLD
                     GADWLRKTKPLKYRHMYLGEMVGSGTQVFDNIVSRKITAREITGFDNIISGVDWGYYP
                     DPWVFIRTYYHAGTRTLYIFDEARGNKLQNDYTARLVKERVAPGEL"
     gene            complement(953..1264)
                     /locus_tag="ACZPFK_11120"
     CDS             complement(953..1264)
                     /locus_tag="ACZPFK_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406907.1"
                     /translation="MRTKSSSIEGLLGAIEQQKNDRDFNILKDLKMLEQQYTKTSKRD
                     YLQLLRKLIDKYEAEEMATVHAALLKKVQAGDLDAIRLWNDMQKESGSSAAEVSIVDS
                     I"
     gene            complement(1533..1733)
                     /locus_tag="ACZPFK_11125"
     CDS             complement(1533..1733)
                     /locus_tag="ACZPFK_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406908.1"
                     /translation="MDKIKKALVHKLLQYRPPQGTELCERCVWPLGAGDRRLCPFPHC
                     VRRYGWNFKEDLKGLKNSTKKP"
     gene            complement(1726..>2222)
                     /locus_tag="ACZPFK_11130"
     CDS             complement(1726..>2222)
                     /locus_tag="ACZPFK_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014417.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HIR1406909.1"
                     /translation="TRSWAAAAAPLRDVCGELRRVGIQGRAPAERRMIRRRGAWREEE
                     LLLLPSYVFAGLDYTAAAYHRAQAIPGVIRWLGMASGAPLPLDTADVLRWGLDSPATM
                     LPSTVLFEGGQWCVVNGPLLRFAGEIVKMDRRQRRATVVTRLGGSEQTIHFGIISQEA
                     PSDG"
BASE COUNT          382 a          732 c          612 g          496 t
ORIGIN      
        1 cagttcgccc ggggccaccc gttctttcac gagccgggcc gtgtagtcat tctgcagctt
       61 attgccccgg gcctcgtcga agatgtacag cgtacgggtg cctgcgtggt agtatgtgcg
      121 gatgaacacc cacgggtcgg ggtagtaccc ccagtccacg ccgctgatga tgttgtcgaa
      181 gcccgtgatt tccctcgccg tgatcttccg gctcacgatg ttgtcgaaca cctgcgtgcc
      241 gctgcccacc atctcgccca ggtacatgtg ccggtatttg agcggcttcg tcttccgcag
      301 ccagtccgca ccgtccagga actccttgcc cagccactcc cgcggcgcgt ccagatacga
      361 cgaatgatgc accagcttac ccggctgttg ctcccgcgca taccggttgg cccagttgcg
      421 tgcgttggcg ggtgggttga agctgacgag cgttaaggtc ggattatccc ccgagccgcg
      481 gaacgcggac tgcttcacgc tcagcaccgc gttttcgccc cgccgcagct ggtccgcttc
      541 ctcaaaccac aagcagccaa tgtacccaaa cttcggctta atgcccttta ttttcatctc
      601 atcatccaag ccgcggaaat agatggtctg gcccgtgggc ttgtagacaa gacgcagcgg
      661 actttttttc tgcaggaagt gttcggtgag ccccaacttt gcgatggccc acagcatctg
      721 ggagtacacg ctgtcctcca gcgtgttgcc catctgccgc atcaccagag cgtggctctg
      781 cggccacttc agcagccaca gcaccacctc tgtgctacag aatgagcttt tcaagctgcc
      841 gcgcccaccg tcttctacca gtgtgtgtgc gcgctgctca cggatcgcac ggtgtgattc
      901 ataaaacgcc gggccgatca cgttctttaa atccaccgtc accgccgggc ggttatatgc
      961 tgtcaacgat gctcacctcc gccgcgctgc tgccactctc tttctgcatg tcgttccaca
     1021 gccggatcgc gtccaggtct ccagcttgca ctttcttcag cagcgccgca tgcaccgtcg
     1081 ccatctcctc agcctcgtat ttgtcaatga gcttgcggag caactgcagg tagtcccgct
     1141 tgctggtctt tgtatattgc tgctccagca ttttaaggtc ttttaaaata ttaaaatcgc
     1201 ggtcattttt ctgctgctca atggccccca aaaggccctc gatgctggat gattttgtcc
     1261 gcatctgctt gtgctccttt ccgcctcgac gtaagaggcc ccaattttgc ccgctgtgcg
     1321 caaaaaggcc ccaggcgggc agtttcaccg cctcggggct ttaaacgttc ttaaacggct
     1381 ttctgccgct tttaaaaggg gattcttggc cttgtgcaaa tcaaagggct attttaggcg
     1441 ggcatgcttc gccacccggc catttctgcc cggttcggtg ctcgctatgc ccgccgatcc
     1501 actccgcagg ggacgtatca accctgcttc ggttacggtt tctttgtact gtttttaagg
     1561 ccttttaaat cttctttaaa attccagcca tagcgtcgca cgcaatgcgg gaaaggacag
     1621 aggcggcggt cgccggcacc gaggggccac acgcaccgct cgcacagctc cgtgccctgc
     1681 ggcgggcggt actgcagcaa cttgtgcacc agggcctttt tgatcttatc catcgctcgg
     1741 cgcctcctgc gagatgatcc cgaaatggat cgtctgctca ctgccgccga gccgcgtcac
     1801 aaccgtggcc cggcgctgtc ggcgatccat cttgacgatc tcgcccgcaa agcgcagcag
     1861 cgggccgttc acaacgcacc actggccgcc ctcaaacaac acggtgctgg gcagcatggt
     1921 ggccgggctg tccaggcccc agcgcagcac gtcggcggta tccaagggca gcggggcgcc
     1981 gctggccatg cccagccagc ggatgacgcc cgggattgct tgggcccggt gataggcggc
     2041 tgccgtatag tcgaggcctg caaacacgta gctgggcagc agcagcagct cctcttcgcg
     2101 ccatgcgccc cggcgccgga tcatacggcg ctcggccggg gcgcggccct ggatacccac
     2161 tcgccgcaac tcgccgcaca cgtcgcgaag aggagctgct gctgctgccc agctacgtgt
     2221 tt
//
LOCUS       DBMDYA010000501         2218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10299_length_2218_cov_1.741615, whole genome shotgun sequence.
ACCESSION   DBMDYA010000501 DBMDYA010000000
VERSION     DBMDYA010000501.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_2218_cov_1.741615"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(271..1335)
                     /gene="ispG"
                     /locus_tag="ACZPFK_11135"
                     /gene_synonym="gcpE"
     CDS             complement(271..1335)
                     /gene="ispG"
                     /locus_tag="ACZPFK_11135"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1406910.1"
                     /translation="MKKGSATMRKLRREVKIGTLAIGGDNPIAVQSMLNVPIRDLAGN
                     VAQAKRLEAAGCQIIRVTVPSAEDARVVSAIKEQVALPLVADIHFDYRAAIAAAEAGA
                     DKIRINPGNIGGDDRVAAVVKACKAKRLPIRIGVNAGSLEKDLLQKYGSPTPEALCES
                     AMRHVRILERHDFEDIVISIKSSHVPTVIAAYRLLAQRCDYPLHIGVTEAGTYRMGLI
                     KSAMGIGGLLLDGIGDTLRVSLTDEPEKEVEAGFDILRAAGFEVSGPEVVSCPTCGRT
                     NIKVAEIAQEVERRLKGCKKPIKVAVMGCVVNGPGEAREADIGIAGGVDSAVLFIKGQ
                     QVRTLRGDIVGQLIEEIGRL"
     gene            complement(1356..>2218)
                     /gene="rseP"
                     /locus_tag="ACZPFK_11140"
     CDS             complement(1356..>2218)
                     /gene="rseP"
                     /locus_tag="ACZPFK_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1406911.1"
                     /translation="LRLLPIGGFVSMEGEDEASDDERSFTRAPVWKRIIVVVAGAVMN
                     MILGFLVLVILVSQQSAITSRTVSQFAENASTQASGLQIGDEILAVNGRRCFIADDVV
                     YEFVRTQDGTADLTVLRDGKKVELPDVRFTTQPLEDGTTSLVIDFKVLPIEKTVFNVV
                     KEAGLWTCSLSRMIFLSLIDLVTGRVAINNLSGPVGIVTVISEAASIGWQPILMILAM
                     ITVNLGIFNLIPFPALDGGKLFLLLIEAIRRKPLPEKYEIVINLVGFAALMCLMLFVT
                     YNDIARLVTG"
BASE COUNT          383 a          761 c          669 g          405 t
ORIGIN      
        1 ggtgagatag acggcgtttt gggcgcgctc caccgcaaca gcctccacgg ccgcgccctc
       61 cagcttgtcc ccgatctgtc cggccacctc gggccagagc tttgacacca atgcctccaa
      121 accgtctcta cgctcccctg cttctatact ttgacgatgc gccccgtcca gggcggcccg
      181 gcggacgccg tgccacaaaa aacgctctct gcccggctgc ccgccgcggc ggcaaaagag
      241 ccgcgcgcct ttgccgcgca cggcccggtt ctataagcgt ccaatctcct cgatcagttg
      301 gcccacgatg tcgccgcgca gggtgcgcac ctgctgcccc ttgataaaga gcaccgccga
      361 atccaccccg ccggcgatgc cgatatccgc ctcccgcgcc tcgccggggc cgttcacgac
      421 gcagcccatg accgcgacct tgatgggctt tttgcagccc ttgagtcgcc gttccacctc
      481 ctgggcgatc tcggccacct tgatgttggt gcgcccgcag gtggggcagc tgacgacctc
      541 cggcccggac acctcaaagc cggcggcgcg caggatgtcg aaccccgcct ccacctcttt
      601 ttcgggctcg tcggtcagcg acacccgcag cgtgtccccg atgccgtcca gcagcaagcc
      661 gccgatgccc atggcggatt tgatcagccc catgcggtag gtgcccgcct cggtcacgcc
      721 gatatgtagc ggatagtcgc agcgctgcgc cagcagccgg taggccgcga tgacggtggg
      781 cacatggctg gacttgatgc tgatgacgat atcctcaaaa tcatgccgct ccagaatgcg
      841 cacgtggcgc atggcgctct cgcagagcgc ctccggggtg gggctgccgt acttttgcag
      901 cagatccttt tccagactgc cggcattgac cccgatgcgg atgggaagcc ttttcgcttt
      961 acaggctttc accactgccg ccacccggtc gtcgccgccg atattgcccg ggttgatgcg
     1021 gatcttgtcc gccccggcct cggctgcggc aatggccgcc cggtagtcga agtggatatc
     1081 cgccaccagc ggcagcgcga cctgctcttt gatggcggat accacgcgcg cgtcctcggc
     1141 cgagggcacc gttacccgga tgatctggca tcccgccgcc tccagccgct tggcctgggc
     1201 cacattgccc gcgaggtcgc ggatggggac gttgagcata ctctgcaccg cgatggggtt
     1261 gtcaccgccg atcgccagcg tgccgatctt cacttctctg cgcagtttcc gcatggttgc
     1321 acttcccttt ttcatttcag cgccgcaagg gcggctcacc cggtgaccag ccgggcaata
     1381 tcgttgtagg tgacaaaaag catcaggcac atcagggctg caaagcccac cagattgatg
     1441 acgatctcat acttttccgg cagcggcttg cgccggatcg cctcgatgag cagcaggaac
     1501 agcttgccgc cgtccagcgc aggaaacggg atgaggttga agatgcccag gttgaccgta
     1561 atcatggcca ggatcatcag aatgggctgc cagccgatgg aggccgcctc gctgatgacg
     1621 gtgacgatgc ccaccgggcc ggacaggttg ttgatcgcca cccggcccgt gaccagatcg
     1681 atcaggctga gaaagatcat gcgggagagc gagcaggtcc acagccctgc ctctttgacg
     1741 acgttgaaga ccgtcttttc gatgggcaga accttgaaat cgatcaccaa gctggtggtg
     1801 ccgtcctcca gcggctgggt ggtgaagcgc acgtcgggca gctccacctt tttgccgtcc
     1861 cgcagcacgg tcaggtctgc ggtgccgtcc tgcgtgcgga caaactcata gaccacgtcg
     1921 tcggcaataa agcagcggcg gccgttgacg gccaagatct cgtcgccgat ctgcaggccg
     1981 ctggcctggg tggaggcgtt ctcggcaaac tggctcaccg tgcggctggt gatggccgac
     2041 tgctgcgaca ccaggatcac cagcaccaaa aagccgagga tcatattcat caccgcgccg
     2101 gcgaccacca cgatgatgcg cttccacacc ggagcccggg tgaaagagcg ctcgtcgtcg
     2161 gacgcctcgt cctccccctc catgctgacg aagccgccga tgggcagcag ccgcagcg
//
LOCUS       DBMDYA010000502         2211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10329_length_2211_cov_1.710900, whole genome shotgun sequence.
ACCESSION   DBMDYA010000502 DBMDYA010000000
VERSION     DBMDYA010000502.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10329_length_2211_cov_1.710900"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="ACZPFK_11145"
     CDS             <1..566
                     /locus_tag="ACZPFK_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024117.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1406912.1"
                     /translation="RNEAETPLRRPSFMYSSGLSAAERGTALHAVLQYADFAAARRDL
                     AAELERLVREGRVSEVTAASADRAALEAFFRSPLMERMLSADRLLREYAFFTRIPAGS
                     AAALAGPLAYEPVLVQGVADCVIEKDGELFLVDYKTDRHRTPEQLRDLYAPQLALYRE
                     ALQRRLGLPVARCSLYAFALGREIEVF"
     gene            1037..1942
                     /locus_tag="ACZPFK_11150"
     CDS             1037..1942
                     /locus_tag="ACZPFK_11150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR1406913.1"
                     /translation="MKILNLGSLNIDKVYSVGHFVSAGETISSANMETFSGGKGLNQS
                     IALARAGAEVYHAGAVGPDGEGLRALLEGSGVHVDYLQTLGTVTGHAVIQLTPKGQNC
                     IIISAGANGELTESYIDGVLEHFAPGDLLLVQNETNNVAYAMRQAKWKGMKIAFNASP
                     ISEALLGYPLELVDYYLINEVEGKALAGGEESGNEEILAGLKARFPEAAIVLTVGKDG
                     GLYQYKDERAGHGIYDVPVADTTAAGDTFCGYFLACVAKGLPAAEGLRMASLASSLAV
                     SQKGAANSIPSWDEVAAFGKTAKSL"
     gene            2010..>2211
                     /locus_tag="ACZPFK_11155"
     CDS             2010..>2211
                     /locus_tag="ACZPFK_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1406914.1"
                     /translation="MAKHLTAPTPHIQAQVGEIAETVLLPGDPLRAKFIADTYLENVK
                     QFNATRNMFGFTGTYKGRPVSVM"
BASE COUNT          436 a          663 c          732 g          380 t
ORIGIN      
        1 agcggaacga ggccgaaacc ccgctgcggc gtccctcttt tatgtattcg tccggcctct
       61 cggccgccga gcggggcacc gccctgcacg cggtgctgca gtacgccgat ttcgccgccg
      121 cgcgccgcga tctggcggcc gagctggagc ggctggtgcg ggaggggcgg gtgagcgagg
      181 tcacggcggc ttccgccgac cgcgccgcgc tggaggcctt tttccgctct ccgctgatgg
      241 agcggatgct ctccgccgac cgcctgctgc gcgagtacgc gttctttacc cgcatcccgg
      301 cgggcagcgc cgccgcgctg gcggggccgc tggcctatga gccggtgctg gtgcagggcg
      361 tggccgactg cgtgatcgaa aaggacggcg agctgttttt ggtggactat aagaccgacc
      421 gccaccgcac gccggagcag ctgcgcgacc tctatgcccc tcagctggcg ctctaccgcg
      481 aggcgctgca gcgccgcctg ggtctgcccg tggcccggtg cagcctctat gcgttcgccc
      541 tcggtcgcga gatcgaggtc ttttgaccgg aacggcccgc ccgtctgccc ggcaaaaggg
      601 gcctggggca cagcggcttt gactcgtgcc tgcgggcagg agagaaaccc gcgcggcggg
      661 ccgaagacga aaatgaaaaa ttgcaggacc ggttctcggc gccatgcggc gcagggggcc
      721 ggttttttgc gccgtctctc ttttctcccg cctctgcgtc ttgccgtttg ccaccgcggt
      781 ttacggcttt tttcgccgga aaagcagatg ctttaacagg gcgttgtagc attttgcaca
      841 tagaatagaa aagaaattga cagaatcacc aaaaaagaaa aaatccgttg acaaggattg
      901 agacgaatga tatattctaa tcacaagata gtttagcagt taaacagtca atgtgatgaa
      961 ctaaaatgaa gcgttgtccc ggctcaaaga aaaagcgccg cggagaaacg ctgcaaaagg
     1021 ggaagaagag gaagccatga agattttgaa tctgggctcg ctgaacattg acaaggtgta
     1081 cagcgtgggg cactttgtga gcgccggcga gacaatctcc tctgccaata tggagacgtt
     1141 cagcggcggc aagggcctga accagtcgat cgctctggcc cgcgcgggcg ccgaggtgta
     1201 ccacgcgggg gccgtcggcc cggacggaga gggcctgcgg gcgctgctgg agggatccgg
     1261 cgtgcacgtc gactacctgc agacgctggg cacggtgacc ggtcacgcgg tcatccagct
     1321 gacccccaag ggacagaact gcatcatcat ctcggcgggc gccaacggcg agctgaccga
     1381 gagctacata gacggcgtgc tcgagcactt tgcgcccggc gacctgctgc tggtgcagaa
     1441 cgagaccaac aacgtggcct acgccatgcg ccaggcaaaa tggaaaggca tgaagatcgc
     1501 tttcaacgcc tcccccatca gcgaggcgct gctgggctac ccgctggagc tggtggacta
     1561 ctacctgatc aacgaggtgg agggcaaggc gctggccggc ggcgaagaga gcggcaacga
     1621 ggagatcctg gccgggctga aagcccgttt cccggaggcg gctatcgtgc tgacggtggg
     1681 caaggacggg ggcctgtacc agtacaagga cgagcgcgcg ggccacggca tctacgacgt
     1741 gccggtggcg gacaccacgg ctgccgggga cacgttctgc gggtatttcc tggcctgtgt
     1801 ggcaaagggg ctgcccgccg cggagggtct gcggatggcg agccttgcca gcagcctggc
     1861 ggtgagccaa aagggcgccg ccaacagcat ccccagctgg gatgaggtcg ccgctttcgg
     1921 caagaccgcc aagagcttgt gagagatttc aagattgcag cggcgctttg ccgcttcaaa
     1981 atacccgatt gcagaaagga atatggatta tggctaaaca tctgactgcc cccaccccgc
     2041 acatccaggc ccaggtcggc gagatcgccg agaccgtact gctgcccggc gacccgctgc
     2101 gggcaaaatt tatcgcggat acttatcttg agaacgtgaa gcagttcaac gccacccgca
     2161 atatgttcgg cttcaccggc acctacaagg gccgccccgt ctcggtgatg g
//
LOCUS       DBMDYA010000503         2207 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10353_length_2207_cov_1.623457, whole genome shotgun sequence.
ACCESSION   DBMDYA010000503 DBMDYA010000000
VERSION     DBMDYA010000503.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10353_length_2207_cov_1.623457"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="ACZPFK_11160"
     CDS             complement(<1..738)
                     /locus_tag="ACZPFK_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1406915.1"
                     /translation="MYTVFDPHFLHLSGKTEDYLDLAVQYGIGGIGVDSELLDDEPRA
                     LEVAKQVWDRGLGWSIMFTPVDFYAPATDDQIFEEGLECFKRRCAVAEKMKVKYCYNH
                     VWSSNADRAYDENFEWHIRRLRRVQQVCEDHGIFYGLEFLGPREVLYRFAHPFIHTIA
                     GVTALADAAGGKAGFLFDTYHWSCEGARTDDLYFALAHVDRMCGFHVNDGVFGRTLPF
                     QRDMERAMPLTTGVIDAATPYKLFQRAG"
     gene            complement(754..>2207)
                     /locus_tag="ACZPFK_11165"
     CDS             complement(754..>2207)
                     /locus_tag="ACZPFK_11165"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     D-xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR1406916.1"
                     /translation="QKADVDPRRIIGIGIDFTSCTMLPLDEELVPLCYNPLYRKSPHS
                     WPKLWKHHAAQQQADRINQLARARGERFIKRYGNASSCEWMFAKILETLEKAPEVYTA
                     THRFVEAADWIVYLLTGTLCKSSVNAGFKAFWSSDEGYPSEDFFAALHPAMKNVIAEK
                     IGPEVHGISAPAGHLCPRMAQATGLSTDTMVAVGNIDAHISFPATGATQDSTMLMIMG
                     TSLCHILISSRETQINGIGGIVRDGVLPGFFGYEAGQAAVGDIYDWFVSNAVPSEYLE
                     EVQKRNINIYDLMNEKMARLAPGASGVLALDWWNGNRSLLVDSDLSGLLLGVTLSTTA
                     EDIYRALVEATAFGSRMIVEAFEAGGVPVSQVYACGGLAFKAPQVMQIFSDILGKPIA
                     ISSIKQTSALGAAMYGAVAAGRSRGGYDTIFEAAERMTRPPSVTYRPNPAYREIYGRL
                     YREYVKLHDYFGKGPNHVMQTLRQIKNDVCTAG"
BASE COUNT          481 a          639 c          626 g          461 t
ORIGIN      
        1 tcctgcccgt tgaaacagct tataaggggt agcggcgtca atcacccctg tggtcagcgg
       61 catagcgcgt tccatatccc gctggaaagg aagcgtgcgg ccgaaaacgc cgtcgtttac
      121 atgaaaacca cacatgcggt ccacatgggc caacgcaaag tagaggtcgt cggtgcgcgc
      181 gccctcgcag gaccaatgat aggtgtcaaa aaggaaacct gcttttccgc cggcggcatc
      241 ggccagtgcc gtcacaccgg caatggtgtg gatgaaaggg tgtgcaaaac gatagagcac
      301 ttcacggggg ccgagaaact caagcccgta aaaaatgccg tgatcctcac agacctgctg
      361 tacacggcgc agacgccgga tatgccattc aaaattttcg tcgtaggcgc ggtctgcgtt
      421 gctggaccag acatgattgt agcagtattt cactttcatc ttttcggcaa cggcgcagcg
      481 ccgcttaaag cattcaagcc cctcttcaaa aatttgatcg tctgtggcag gggcataaaa
      541 atctaccggt gtaaacatga tgctccagcc caggccgcga tcccatacct gctttgcgac
      601 ctctagggcc cggggttcgt cgtccagcag ctccgagtcg acgccgattc ctccaatacc
      661 atattgaacc gccaaatcca gatagtcctc agtttttccg gaaagatgta aaaagtgggg
      721 gtcaaaaaca gtgtacataa aagtcaactc ctttcatcct gctgtgcaga catcgttttt
      781 gatctgacgc agcgtttgca tgacgtgatt gggccctttg ccgaaataat cgtgcagctt
      841 cacgtattcc cgatagagtc gcccgtagat ctcccgatag gcaggattgg ggcggtaggt
      901 gacggaggga ggacgggtca tccgctccgc ggcctcaaag atcgtgtcat agccaccgcg
      961 cgaacggccg gccgcgacgg cgccatacat cgccgccccc agggcggagg tctgcttgat
     1021 gctggagatg gcgatcggtt tgccgagaat atccgaaaag atctgcatca cctgcggcgc
     1081 tttgaaggcg agaccgccgc aggcatatac ctgcgagacg ggcacgccgc cggcctcgaa
     1141 cgcctcgacg atcatgcgcg agccgaatgc cgtggcctcc acaagggcgc ggtagatatc
     1201 ctccgctgtg gtggacagcg tcacccccag caaaagcccg ctcaggtcag aatccaccag
     1261 cagtgaccgg ttgccgttcc accagtccag cgccagcaca ccggaagcgc cgggcgccag
     1321 ccgcgccatt ttttcattca tcaggtcgta gatgttgatg ttgcgctttt gcacctcttc
     1381 cagatactcc gagggcaccg cattgctcac aaaccagtca tagatatctc ctactgccgc
     1441 ttggccggct tcatagccga aaaatcccgg cagaacgccg tcccgcacaa tgccgccgat
     1501 gccgttgatc tgagtctcgc gggaggaaat caggatgtgg cacagcgagg tgcccataat
     1561 catcagcatg gtggaatcct gagtggcccc ggtggcggga aaggaaatat gtgcgtcaat
     1621 gttgcccact gcgaccattg tatcggtgct caggcccgtg gcttgcgcca tccgcgggca
     1681 gagatgcccg gcgggggccg agatgccatg tacctccggg ccgatttttt ccgcgattac
     1741 atttttcatg gccgggtgga gagctgcaaa aaaatcttca gaaggatagc cctcgtcgct
     1801 gctccaaaat gccttgaaac cggcgttcac actgcttttg cagagtgtgc ctgtgagcag
     1861 gtaaacgatc cagtcggcgg cctccacgaa gcggtgggtt gcggtatata cctccggcgc
     1921 tttttccaat gtctccagga tcttggcgaa catccactcg cacgaggacg cgtttccata
     1981 tcgcttgata aagcgctcgc cgcgcgccct tgcaagctga ttgatcctat cggcttgctg
     2041 ctgtgccgcg tgatgcttcc acagcttcgg ccagctgtgg gggcttttcc ggtacagggg
     2101 gttataacaa agcggaacga gctcttcgtc cagagggagc attgtacagg aggtgaaatc
     2161 aatgccgatg ccgatgatcc gccggggatc gacgtcggct ttctgcc
//
LOCUS       DBMDYA010000504         2205 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10367_length_2205_cov_1.423004, whole genome shotgun sequence.
ACCESSION   DBMDYA010000504 DBMDYA010000000
VERSION     DBMDYA010000504.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10367_length_2205_cov_1.423004"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            721..>2205
                     /locus_tag="ACZPFK_11170"
     CDS             721..>2205
                     /locus_tag="ACZPFK_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406917.1"
                     /translation="MSARRTGEDETTGIWRKYEAGRDHHNGEGMYRRVERCHRFYEGD
                     QWAGIKAGGEELPMLNFIRPICTYKIATVAMVDTAIIYSAMDGDPQAAAVCEALTRFA
                     AVQWEKGKLDSKKWAVIKNACIAGDHYLYCYDARQPSESVARERTPQLRMRLVGKTSL
                     YLADEQEPDLNSQEWILIAERRPVAQVREEARKNGLAEAEIARIAADEVDETGVGYTG
                     AQEVKSDLGKCTSLLYMEKTPEGLKFCRSTETVVYQPEQLVPGLDVYPVCGMRWSERM
                     GSARGVGVVEGLIPNQIEVNRTMARRAVCVKRYSFPTAVYDQDRVANPEALGKVGASL
                     RVKNLAGNPLSSMVQYLTPVGIGGDAAALQGEIVAMSRELEGAGDAATGQVDPTKASG
                     EAIKAARDQSALNLNEQTAAYRQFAEDLALVWYKLWAAYSTDGMAVRLPDGRGGETET
                     VIPHEVLAGLDVDIRIDISPVDPYSVLSRGLALENALAHGHITFE"
BASE COUNT          468 a          685 c          812 g          240 t
ORIGIN      
        1 cggtacgacg ccatcctggc gaacatcgac gcctacgacg gtacggcgga ggggcagcgg
       61 gatgtttaaa agagaaaagc cgcccttggc ggcacgggcc cacgcggcgg cccctgacag
      121 gacggccgga ccgggcgcgg cggtgaaagt atccgccgag aacaaaggcg gcccgggcgg
      181 gttgccgaga ggttccagcg ggggcccaaa aggcccggcg cggggccaaa gcggctcggc
      241 caacgataaa ggcggcccgg ccgggttgcc gagaggttcc agcgggggcc caaaaggctc
      301 gggtggagac gaaagcggct cgatcaggga cggaagaaac ctgggcggga acgagagcgc
      361 tccgaccggg aacaaaagcg tcccggccgt tgataaaaac gtcccggccg ttgataaaag
      421 aagcccgatc gcgaacgaaa gaagccaggg cggcgatcca aaagtcccaa ccagcgacaa
      481 aaacggcccg ctcgttgaca aaagaacccc ggccgttgac aaaagcgccc cggccgttga
      541 aaaaagaagc ccgatcaggg acggaagaaa cccgggcggg gagcaaagcg cccccatcag
      601 cgacaaaagc gtcccgggcg ggaacaaaag cggtccgagc gacgacaaaa acgtcccgga
      661 cgttgacaaa agcgccccgg acggccgggc aaaacgggag acgaaaaagg aggcgatggc
      721 gtgagcgcac ggcggacagg ggaggacgag accaccggga tctggcgcaa atacgaggcg
      781 gggcgcgacc accacaacgg cgagggcatg taccgccggg tggagcgctg ccaccgcttt
      841 tatgagggcg accagtgggc gggaatcaag gcgggcggcg aagaactgcc gatgctgaac
      901 ttcatccggc ccatctgcac ctacaagatc gccaccgtgg ccatggtgga cacggccatc
      961 atctactcgg ccatggacgg ggacccgcag gccgccgcgg tctgcgaggc gctgacccgc
     1021 tttgcggccg tccagtggga aaaaggcaag ctggacagca agaagtgggc ggtcatcaaa
     1081 aacgcctgca tcgcgggcga ccactatctg tactgctacg acgcgcggca gccctccgag
     1141 agcgtggccc gcgagcgcac gccccagctg cggatgcggc tggtgggcaa gaccagcctt
     1201 tatctcgccg acgagcagga gccggacctc aatagccagg agtggatcct catcgccgag
     1261 cggcggccgg tggcccaggt gcgggaggag gcgcgcaaaa acggcctggc ggaggccgag
     1321 atcgcccgca tcgcggcgga cgaggtggac gagaccggcg tgggctacac cggcgcccag
     1381 gaggtgaagt ccgatctggg caaatgcacc agcctgctct acatggaaaa gacccccgag
     1441 gggctgaagt tctgccgctc caccgagacg gtggtctacc agcccgagca gctggtgccg
     1501 gggctggacg tttacccggt gtgcggcatg cgctggagcg agcggatggg cagcgcccgg
     1561 ggcgtggggg tggtcgaggg gctcatcccc aaccagatcg aggtgaaccg caccatggcc
     1621 cggcgggccg tctgcgtgaa gcggtacagc ttccccaccg cggtgtacga ccaggaccgg
     1681 gtggcaaacc cggaggcgct gggcaaggtg ggggccagcc tgcgggtgaa aaacctggcg
     1741 ggcaacccgc tgagcagcat ggtgcagtac ctgacgccgg tgggcatcgg cggcgacgcg
     1801 gcggcgctgc agggcgagat cgtggccatg agccgcgagc tggagggcgc gggcgacgcg
     1861 gccaccggcc aggtggaccc caccaaggcc agcggcgagg ccatcaaggc ggcgcgggac
     1921 cagagcgcgc tgaacctgaa cgagcagacc gccgcctacc gccagttcgc cgaggatctg
     1981 gccctggtgt ggtacaagct gtgggccgcc tattcgaccg acggcatggc cgtgcggctg
     2041 cccgacggcc ggggcggcga gaccgagacg gtcatcccgc acgaggtcct ggccgggctg
     2101 gacgtggaca tccgcatcga catctcgccg gtggacccct acagcgtgct cagccgcggg
     2161 ctggcgctgg aaaacgcgct ggcccacggg cacatcacct ttgag
//
LOCUS       DBMDYA010000505         2195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10415_length_2195_cov_1.216332, whole genome shotgun sequence.
ACCESSION   DBMDYA010000505 DBMDYA010000000
VERSION     DBMDYA010000505.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_2195_cov_1.216332"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="ACZPFK_11175"
     CDS             <1..645
                     /locus_tag="ACZPFK_11175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1406918.1"
                     /translation="DYSDWCHLTSIEKYAQNHKIMVVMPSVANSFYQDMFIGPKYLTF
                     LTEELPAYVSLLFPASSRREDNFVAGLSMGGYGAWHVALAKPEQYAAAASLSGARSFP
                     YGMEDRLADPASPWPFRAIFESCDIDKLAASEVNLLVQIKNILAAKKPLPRLFQTVGT
                     EDFTYEVNQQMRRALEQLGVEFTYEEHPGIHDWDYWDAHIQRALDWMPLANTTV"
     gene            complement(888..1190)
                     /locus_tag="ACZPFK_11180"
     CDS             complement(888..1190)
                     /locus_tag="ACZPFK_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6506 family protein"
                     /protein_id="HIR1406919.1"
                     /translation="MEKRKFAFLLKGEDYDPIVQQTFFETPFMTTYVFTVRNFEEAAA
                     KVQALAEEGFGVIELCGAFGPEKAREYQQLTGGRVAIGYVTHSPEQDHLFAAFFGE"
     gene            complement(1361..1702)
                     /gene="rplQ"
                     /locus_tag="ACZPFK_11185"
     CDS             complement(1361..1702)
                     /gene="rplQ"
                     /locus_tag="ACZPFK_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR1406920.1"
                     /translation="MPGTRKLGRTSDSRRAMLRAMVTYLFEKGKIETTVTRAKEVRAV
                     AEKMITLGKQDSLHAKRQIYSFITKEDVAKKLIDELGPKYADRNGGYTRIIKIGARRG
                     DAAEMAVIELV"
     gene            complement(1776..>2195)
                     /locus_tag="ACZPFK_11190"
     CDS             complement(1776..>2195)
                     /locus_tag="ACZPFK_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR1406921.1"
                     /translation="NYTVENTRVGQITDYDKLTLEVWTDGIINAKEAVSLAARIMTEH
                     LNLFVNLSQEAMDAEIMVEKDDKGKEKVLEMTIEELDLSVRSFNCLKRAGINTVEDLI
                     SKSEDEMMKVRNLGRKSLEEVISKLESLGFKLSKDDE"
BASE COUNT          412 a          703 c          569 g          511 t
ORIGIN      
        1 gattactccg actggtgcca cctcaccagc attgaaaaat acgcccagaa ccacaagatc
       61 atggtcgtca tgccctcggt ggccaacagc ttttatcagg atatgttcat cgggccaaaa
      121 tacctcacct ttctcaccga ggagctgccc gcctatgtca gcctgctgtt ccccgccagc
      181 agcagacggg aggacaactt tgtcgccggg ctttccatgg gcggttacgg cgcctggcat
      241 gtggctttgg caaagccgga gcaatacgcc gccgcagcca gcttgtcggg ggcacggagc
      301 ttcccctacg gcatggagga tcgccttgcc gatcccgcct cgccctggcc gttccgcgcc
      361 atctttgaaa gctgcgatat cgacaagctg gccgcctccg aggtcaacct gctggtccag
      421 atcaaaaata tcctggccgc caaaaagccc ctcccccgcc tgttccagac cgtgggcaca
      481 gaggacttca cctatgaggt caaccagcag atgcgccgtg ccctggagca gctgggcgtg
      541 gagttcacct atgaggagca tcccggcatc cacgactggg actattggga cgcccatatc
      601 cagcgcgccc tggactggat gccgctggca aacaccaccg tttgagcttc ttttacaggg
      661 ctcgtcaaac cgggacaaga caaaacagag cgtgatattt tttaaaaata tcacgctctg
      721 tttttaattg aagacccgct aaaccgggca catcatcggg atgcagccaa caaggcggtc
      781 catcgtcatc cccgcggtca atgccgttct gttgccacgc cgccctgttg ccctgccgtc
      841 ccgctgtcat gtcgccctgt cgttatgcca ttcgattgct ctatcgctca ctcgccgaaa
      901 aaagccgcga acaagtggtc ctgctccggg ctgtgggtga cgtatccgat cgccactctg
      961 cccccggtga gctgctgata ctcccgggct ttttcagggc caaaggcgcc gcagagctcg
     1021 atgacgccaa agccctcctc cgccaacgcc tgcaccttgg cggccgcctc ttcaaaattg
     1081 cgcactgtga acacataggt cgtcataaag ggcgtctcaa agaatgtctg ctggacgatc
     1141 gggtcatagt cctcgccctt gagcaaaaaa gcaaattttc tcttttccat ctttcagtcc
     1201 ctcctttctg ttccgccgtc gtcgtcccgg cccatcccgg gctcttttca gccctattgt
     1261 agcacgcggc gcgttcggat gcaaaaaaac ttcccgccca cggcggcccc ggcggggccg
     1321 tttctgtgcg ggaagttttc agttgggctc aggctgtcgc tcagaccagc tcgataaccg
     1381 ccatctcggc agcgtcgccg cggcgcgcgc cgatcttgat gatgcgggta tagccgccgt
     1441 tgcggtcggc atacttcggg ccaagctcgt cgatcagttt tttggcaaca tcctctttgg
     1501 tgataaaaga atagatctgc cgtttcgcat gcagggagtc ctgcttgccg agtgtgatca
     1561 tcttttcggc aacagcgcga acttctttcg ctcgggtaac cgtggtctcg atcttgcctt
     1621 tttcaaaaag gtaggtgacc atcgccctga gcatagccct gcggctatcg ctggttctgc
     1681 caagctttct tgtaccgggc atcccaattc actcctttgc agtaatcagg gaaccggcgg
     1741 caggcactgg gccgcgccgc gttcctccgt gtcttttact cgtcgtcctt actcagcttg
     1801 aagccgagag attccagctt ggagatgacc tcctccaggc tcttgcggcc caggttgcgc
     1861 accttcatca tctcgtcctc gctcttgctg atgaggtctt cgaccgtatt gatgcccgcg
     1921 cgcttgaggc agttgaacga acgcaccgac aaatccagct cctcgatcgt catctcgagg
     1981 accttttcct tgcccttgtc gtccttctcc accatgatct cggcgtccat cgcctcctgg
     2041 gacaggttga caaacaggtt cagatgctcg gtcatgatcc gcgcggcaag gctgaccgcc
     2101 tctttcgcgt tgatgatgcc gtcggtccaa acctccagcg tcagcttgtc gtagtcggtg
     2161 atctggccca cacgggtatt ctccacagta tagtt
//
LOCUS       DBMDYA010000506         2193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10427_length_2193_cov_1.390057, whole genome shotgun sequence.
ACCESSION   DBMDYA010000506 DBMDYA010000000
VERSION     DBMDYA010000506.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_2193_cov_1.390057"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="ACZPFK_11195"
     CDS             complement(<1..394)
                     /locus_tag="ACZPFK_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MntP/YtaF family protein"
                     /protein_id="HIR1406922.1"
                     /translation="MEGMDLSGFLPGLVMAAALTTDSFVAGLGLGAQEIRVPFFSALI
                     VSVMGTGCLAASLLLGDLLEPVLSQSAAKAVSFLLLFGLGAFKFFESTLKIALRRHGG
                     RGSVRFTALGLQFLLAVYACPEEADADHS"
     gene            595..>2193
                     /locus_tag="ACZPFK_11200"
     CDS             595..>2193
                     /locus_tag="ACZPFK_11200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1406923.1"
                     /translation="MKQKKKLLRLAALVAAAAAAVGAMLIWYSRALSARLEDEIIGTL
                     AEVSGQSAVLLRGEVENMTGTLEDLASVLGQWEEWDPRRAVEELKQVAQNHGLKRMGI
                     ITADGHTFATDGAQLDLAARAYFEAGLQGESRVSVGLSDQAGGGRITVYSVPVHRGEE
                     VLAVLFATADTSRYLPMVKLDLFDGQGVCYLVEGDGAPIMDSGSGALAGGFDNFFEAL
                     AAASADGVQTAAKLRDAVQAGGSGYLTLQSGGTQKYLYYQPVGLNGWYLFTLAPTAVL
                     GRQRASILHSAYLLCLVITLVFAALLTLLLRMQRQNQRRLEELVYVDPVTGGWSHERF
                     CREARRALDAAGAAHWAVWDLDIQYFKYINDIYGHEEGDRALRYIWGCLSACADGRGL
                     FARRSADRFELLLPYGDPAALPRQLESFCALLHSGRAQRRYELSPAIGVCEIDDPSLE
                     VEELLDRAQLARKAIKKGQPGPWAFYDEAMRRGRNREREMEDRMEASLREGGFFVLLQ
                     PEYTADGGRPSGAEALVRWRGPDGG"
BASE COUNT          365 a          754 c          722 g          352 t
ORIGIN      
        1 tgctgtggtc ggcgtcggcc tcctcggggc aggcgtatac ggccagtaaa aactgcagcc
       61 ccagcgcggt aaatcgcacg ctgcccctgc ccccgtgccg tcgcagggcg atcttcagcg
      121 tgctctcaaa aaatttaaaa gccccgaggc cgaacaacag caaaaagctg accgcctttg
      181 ccgccgactg cgacagcacc ggctccagca aatcgcccag cagcagcgag gcggcaaggc
      241 atccggtacc catcaccgac acgatgagcg cgctgaaaaa cggcacccgt atttcctggg
      301 cgcccagccc cagccccgcc acaaacgagt cggtggtcag cgcggcggcc atcaccagcc
      361 ccggtaaaaa accgctgaga tccatcccct ccaccccgct ctccgccgcc cctctgccgg
      421 ggccgctcat agaccagagt atgcgccgcc cggcaaaagc gtccgtgaaa aagccgttcc
      481 cgttttgctc ccgccgccag cgggactttg tcccgctttt ctgttataat ggggacagac
      541 tttccctgtc ccgcgaccag gaggcggggc tgatccacga aagggggccg aaacgtgaaa
      601 cagaaaaaga agctgctgcg tctggccgcg ctggtggccg cagctgcggc cgcagtgggc
      661 gccatgctga tctggtactc ccgtgcgctc tcggcccggc tcgaggacga gatcatcggc
      721 accctggccg aggtctcggg ccagagcgct gttttgctgc gcggcgaggt ggaaaacatg
      781 accggcacgc tggaagattt agccagcgtt ctcggccaat gggaggaatg ggacccccgc
      841 cgggcggtgg aggaactgaa acaggtggcg cagaaccacg gcctcaagcg catgggcatc
      901 atcactgcgg acggacacac ctttgccacc gacggcgcgc agctggacct ggccgcgcgg
      961 gcctacttcg aggctgggct gcagggcgag agccgcgtct cggtcggcct gtccgatcag
     1021 gcggggggcg gccgcatcac cgtctacagc gtccccgtcc accgcggcga ggaggtgctg
     1081 gccgtcctct tcgcgaccgc ggatacctcc cgctatctgc ccatggtcaa gctggacctc
     1141 ttcgacggcc agggcgtctg ctatctggtg gagggggatg gagcccccat catggactct
     1201 ggcagcggcg cgctggccgg cggcttcgac aacttctttg aggcgcttgc cgccgcctcc
     1261 gccgacggcg tccagacggc ggcaaagctg cgcgacgccg tacaggccgg cggctccggt
     1321 tacctgaccc tgcagagcgg cgggacgcaa aaatatctct actatcagcc ggtggggctc
     1381 aacggctggt atcttttcac cttggcgccc accgccgtcc tggggcggca gcgcgcctcc
     1441 atcctccact cggcctatct gctctgtctc gtcatcaccc tggtcttcgc cgcgctgctg
     1501 acgctgctgc tgcgcatgca gcgccaaaat caacgacggc tggaagaact ggtctatgtc
     1561 gaccccgtca ccgggggctg gtcccacgag cggttctgcc gcgaggcgcg gcgcgcgctg
     1621 gacgcggccg gggccgccca ctgggcggtc tgggacctgg acatccaata cttcaaatat
     1681 atcaacgata tctacggcca cgaggagggg gaccgggcgc tgcggtatat ctggggctgc
     1741 ctttcggcct gtgcggacgg ccggggtctt tttgcccgcc gctccgccga ccgctttgag
     1801 ctgctgctgc cttacgggga cccggcggcg ctgccaagac agctggagag cttttgcgcc
     1861 ctgctgcaca gcgggcgcgc ccagcgccgt tatgagctct cccccgccat cggcgtgtgc
     1921 gagatcgacg acccgtccct ggaggtggaa gagctgctcg accgcgcgca gctggcccgc
     1981 aaggccatca aaaaggggca gcccggcccc tgggcttttt acgacgaggc catgcgccgc
     2041 ggccgcaacc gcgagcggga gatggaggac cggatggagg cctcgctgcg ggagggcggc
     2101 ttttttgtgc tgcttcagcc cgaatacacc gccgacggcg gccggccgtc cggggccgag
     2161 gccctggtgc gctggcgcgg gccggacggc ggc
//
LOCUS       DBMDYA010000507         2191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10436_length_2191_cov_1.865072, whole genome shotgun sequence.
ACCESSION   DBMDYA010000507 DBMDYA010000000
VERSION     DBMDYA010000507.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_2191_cov_1.865072"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="ACZPFK_11205"
     CDS             <1..1184
                     /locus_tag="ACZPFK_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406924.1"
                     /translation="RVMELSETYDLRIEPDKKVKEISVPMQQRVEIFKVLWRKAEIII
                     FDEPTAVLTPQEIDEFCEIALKLKEQGKTLLFISHKLGEVMRISDRVTVMRLGKVVCT
                     KDIGDTDEKDLAHLMVGRDIELGGGERKPVEADKTVLAVKGVSMTAGGVKKLDNIDLE
                     VHPGEILGVIGIDGNGQEELVDAICGKARVDGGEILLEGENIAGKSVKEIKDMGLSTV
                     YEDRHKDGLVLKYSVRDNLILGYQDRPQYLRKWKAFLDPKAIQKNAEDLQKAFDIRCG
                     SLEVFAGTLSGGNQQKIILAREVSAKPRLLMVVQPTRGLDMGAIEFVQDKVVEQRNAG
                     CGVLLFTLELDEVLQLSDRIAVIHNGKIIKTVVNKNVTKQQIGRYMLGVLDDGTEEAA
                     Q"
     gene            1181..>2191
                     /locus_tag="ACZPFK_11210"
     CDS             1181..>2191
                     /locus_tag="ACZPFK_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406925.1"
                     /translation="MKKKLDVVKLKQGLGDTVSSLLALLLAFVVSGLIVMIPGANPFE
                     VFASLFKGGLGSGMALAGTLNRMAPILLAGLAITAGNSCGVFNMGFTSQFLMGFLCAG
                     FVGYMVPLPAPLLIPLTILAAVFGAMLWAFFPIMLNIKRNVNIIFSCIMLNYIAKYLL
                     NFLVQKFPGYVPTSGGSPNIQEAAELPYLITKPYKISMGVLFSLLCLVALYVILFKMR
                     TGYEMRAAGLSRTAAYSAGIAVQKKAVAGMLLSGVFTGICAALEVMGTTGRVLEDYDP
                     GYMGLGIAIAMLGKQNPVMILISGFLFAAMKNGTTLMQMNTGISAQFVLVIEGLLIIF
                     LGI"
BASE COUNT          439 a          664 c          684 g          404 t
ORIGIN      
        1 agcgggtgat ggagctctcg gagacctatg accttcgcat cgagccggac aaaaaggtca
       61 aggagatctc ggtgcccatg cagcagcggg tggagatctt caaagtgctc tggcgcaagg
      121 cagagatcat catcttcgac gagcccaccg ccgtgctgac cccgcaggag atcgacgaat
      181 tctgcgagat cgccctcaag ctgaaagagc agggcaaaac gctgctcttc atcagccaca
      241 agctgggcga ggtcatgcgc atctccgacc gggtcaccgt catgcgcctg ggcaaggtgg
      301 tgtgcaccaa ggacattggg gacaccgacg aaaaggacct ggcacacctg atggtgggcc
      361 gcgacatcga gctgggcggc ggcgagcgca agccggtgga ggccgataaa acggttctcg
      421 cggtaaaagg cgtcagcatg accgccggcg gcgtcaaaaa gctggacaac atcgacctgg
      481 aggttcaccc gggcgagatt ttgggtgtga tcggcatcga cggcaacggc caggaagagc
      541 tggtcgacgc catctgcggc aaggccaggg tggacggggg cgagatcctg ctggagggtg
      601 agaacatcgc cggcaaatcc gtcaaggaga tcaaggacat gggcctttcc accgtctatg
      661 aggaccggca caaggacggc ctggtgctga aatactcggt gcgggacaac ctgattctgg
      721 gctatcagga caggccccag tacctgcgca aatggaaagc gtttttggac ccgaaagcga
      781 tccagaagaa cgccgaggac ctgcaaaaag cgttcgacat ccgctgcggc tcgctggagg
      841 tctttgcggg caccctctcg ggcggcaacc agcagaagat catcctggcc cgcgaggtca
      901 gcgcgaagcc ccgcctgctg atggtcgtac agcccacccg cggcctggac atgggcgcca
      961 ttgagttcgt gcaggacaag gtggtggagc agcgcaacgc cggctgcggc gtgctgctgt
     1021 tcacgctgga gctggacgag gtactgcagc tcagcgaccg catcgccgtc atccacaacg
     1081 gcaagatcat caagacggtg gtcaataaga acgtcaccaa gcagcagatc ggccgctata
     1141 tgctgggcgt gctggacgac ggaacggagg aggctgcgca atgaaaaaga aactggacgt
     1201 tgtaaaactg aaacagggcc tcggcgacac ggtctcctcg cttctggcgc tgctgctcgc
     1261 ctttgtggtc agcggcctga tcgtcatgat ccccggcgcc aatccctttg aggtcttcgc
     1321 ctcgctgttc aagggcggct taggctccgg catggccctg gcgggcaccc tcaaccgcat
     1381 ggcgcccatc ctgctggccg gcctcgccat cacggcgggc aacagctgcg gcgtgttcaa
     1441 catgggcttt accagccagt ttttgatggg ttttctctgc gcgggctttg tgggctacat
     1501 ggtaccgctc cccgcgccgc tgctcatccc gctgaccatc ctggccgcgg tattcggggc
     1561 tatgctgtgg gcgttcttcc ccatcatgct caacattaag cgcaatgtca acatcatctt
     1621 ctcctgcatc atgctcaact acatcgccaa atacctgctc aactttctgg tgcagaagtt
     1681 cccgggctat gtgcccacca gcggcggctc gcccaatatc caggaggccg ccgagctgcc
     1741 ctatctgatc accaagccct ataagatcag catgggcgtt ctgttctcgc tgctctgcct
     1801 ggtggcgctg tacgtcatcc tgttcaagat gcgcaccggc tatgagatgc gcgccgccgg
     1861 cctcagccgc accgccgcct actcggccgg catcgcggtg cagaaaaagg cggtggcggg
     1921 catgctgctc agcggagtgt tcaccggcat ctgcgcggcg ctggaggtca tgggcaccac
     1981 cggccgcgtg ctggaggact acgacccggg ctatatgggt ctcggcatcg ccatcgccat
     2041 gctgggcaag cagaacccgg tgatgatttt gatctccggc ttcctgttcg cggccatgaa
     2101 gaacggcacc accctcatgc agatgaacac cggtatctcg gcccagttcg tgctggtcat
     2161 cgaggggctg ctgatcatct tcctgggcat c
//
LOCUS       DBMDYA010000508         2186 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10464_length_2186_cov_1.909832, whole genome shotgun sequence.
ACCESSION   DBMDYA010000508 DBMDYA010000000
VERSION     DBMDYA010000508.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10464_length_2186_cov_1.909832"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="ACZPFK_11215"
     CDS             <1..295
                     /locus_tag="ACZPFK_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1406926.1"
                     /translation="VKYVDAGRPVSTLAVVGGSGGDFIPAALAAGADCLLTGEAGHHD
                     ALDAKRLGLSLVAAGHFATEFPLMPVLGDKLKRRFPELRVLVSKRCRDPFTYL"
     gene            421..1041
                     /locus_tag="ACZPFK_11220"
     CDS             421..1041
                     /locus_tag="ACZPFK_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406927.1"
                     /translation="MREEFDDYQREEQQRSLLEAELAPGEAILWQGAPQGKIRTRSAD
                     LLRVFGLFWLGFSLCWELPILLALFSGYLMGIVYFLFGLPFVGVGIWLAFLRPYRQRR
                     EIESALYAVTDRRVIVLTTYPSRVVRSLPLKAVRSMEKRFRLDGRGELYFGVGSRAHG
                     YEDGYDGGARRRGAVFAFYGVDADAAQCAMQQRLSALRKGADGNGA"
     gene            1031..>2186
                     /locus_tag="ACZPFK_11225"
     CDS             1031..>2186
                     /locus_tag="ACZPFK_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1406928.1"
                     /translation="MALDAATLVLTARELDARLAGARVDKIFQPTRDEVVLMMRSRAG
                     GAKLLLSARSGAARIGVTTESFENPQVPPSFCMLLRKYFSGGRLDGVHAEKGERLVFF
                     DFTCTSEMGDQVPVTLAAELMGRYANIVVVNGERRVVDALKRVDLDASAVRPLLPGLA
                     YTLPPRQDKPDFFETDENEFLRRVFSFDAPAADAVMKAVSGIGPAVCREIAYRALGEG
                     RFFTGELNAGQKQALAAAAAGIRAEFAAGGRPTVVVDETGKPVEYSFTPLTQYQPRCE
                     LRPCESYCGMLEDYYAAKDQAERLRQKSRNLGKTVQNLCERAKRKQAARLEEQAATEK
                     ADHLRVWGELLQANLYAVQRGAKEATVENYYDGAQVTIPLDVRLSPSANAQ"
BASE COUNT          340 a          684 c          799 g          363 t
ORIGIN      
        1 ggtgaagtac gtggacgcgg gcaggccggt ttccacgctg gccgtggtgg gcggctcggg
       61 gggcgatttc atccccgccg cgctggcggc gggcgccgac tgcctgctca ccggggaggc
      121 cggccatcac gacgcgctgg acgcgaagcg gctgggcctc agcctggtgg ccgcggggca
      181 tttcgccacc gagttcccgc tgatgccggt tctgggcgac aagctcaagc gccgctttcc
      241 cgagctgcgg gtgctggtca gcaagcgctg ccgcgacccg ttcacctacc tgtgaaccgg
      301 cgccgcgggc agcctgcgcc gccctcaccg cggtctgctg ccccaggaat ggcagcggag
      361 gacgcgagag gtgaacggcg aaattcacgc gcatgcgcgg gtacttaaaa ggagagacag
      421 atgcgggaag aatttgacga ttatcagcgc gaggaacagc agcgcagcct tttggaggcg
      481 gagttggcgc cgggggaggc catcctctgg cagggggcgc ctcagggtaa gatccgcaca
      541 cggagcgccg acctgttgcg cgtgtttggg ctcttctggc tgggattttc gctgtgttgg
      601 gagcttccga tccttttggc tctgttttcg ggctatctga tggggattgt ttactttctg
      661 ttcgggctgc cttttgtggg cgttggcatc tggctggcat ttttgcggcc gtaccggcag
      721 cgccgcgaga tagagagcgc tttgtatgca gtcaccgacc gacgggtgat agtgctgacc
      781 acctacccct cccgggtggt gcgctcgctg ccgctgaaag ccgtgcgctc gatggaaaag
      841 cgtttcaggc tggacggcag gggcgagctg tactttgggg tgggctccag ggcccatggc
      901 tatgaggacg gctatgacgg cggcgcgcgg cggcgcggcg ctgtcttcgc cttttacggc
      961 gtcgacgccg acgcggccca gtgcgcgatg cagcagcggc tgtccgcgct gagaaagggg
     1021 gcggacggca atggcgcttg acgcggccac cctggtcctg acggcgaggg agcttgacgc
     1081 gcggctggcg ggggcgcggg tcgacaagat ctttcagccc acccgcgacg aggtggtgct
     1141 gatgatgcgc agccgcgccg ggggcgccaa gctgctgctc tcggcccgca gcggggccgc
     1201 ccgcatcggc gtcaccactg agagctttga aaatccgcag gtgccgccct ccttttgcat
     1261 gctgctgcgc aagtattttt ccggcgggcg cctggacggc gtgcacgccg aaaaagggga
     1321 gcgcctggtc tttttcgact tcacctgcac cagcgagatg ggggatcagg tgcccgtcac
     1381 cctggcggcc gagctgatgg gccgctacgc caacatcgtg gtcgtcaacg gggagcgccg
     1441 ggtcgtcgac gccctcaagc gggtggacct ggacgcctcg gccgtgcggc cgctgctgcc
     1501 agggctggcc tatacgctgc ccccgcgcca ggacaagccg gactttttcg agacagatga
     1561 gaacgaattt ctgcggcggg tcttttcctt tgacgcgccg gccgcggacg cggtgatgaa
     1621 agcggtcagc ggcatcggcc cggcggtctg ccgggagatc gcctaccgcg cgctgggcga
     1681 gggacgattt tttaccggcg agctgaacgc ggggcaaaag caggcgctgg cggccgccgc
     1741 cgcaggcatc cgcgccgagt ttgcggcggg cgggcggccc actgtggtgg tggacgagac
     1801 cggcaagccg gtggagtaca gctttacccc cctcacccag taccagcccc gctgcgagct
     1861 gcgcccgtgc gagagctatt gcggcatgct ggaggactac tacgcggcca aggaccaggc
     1921 cgagcggctg cggcagaaaa gccgcaacct gggcaagacg gtgcagaacc tctgcgagcg
     1981 ggcaaagcgc aagcaggccg cccgcctgga agagcaggcc gccaccgaga aagccgacca
     2041 cctgcgggtc tggggcgagc tgctgcaggc caatctctac gccgtgcagc gcggggccaa
     2101 ggaggccacg gtggagaact actacgacgg cgcccaggtc accatcccgc tggacgtgcg
     2161 gctctccccg tcggccaacg cgcagc
//
LOCUS       DBMDYA010000509         2183 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10484_length_2183_cov_1.942363, whole genome shotgun sequence.
ACCESSION   DBMDYA010000509 DBMDYA010000000
VERSION     DBMDYA010000509.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10484_length_2183_cov_1.942363"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(274..1515)
                     /locus_tag="ACZPFK_11230"
     CDS             complement(274..1515)
                     /locus_tag="ACZPFK_11230"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1406929.1"
                     /translation="MKILVINCGSSSLKYQLIDMDGEKVLCKGNCERIGIPGGMITHK
                     TADGYEIKVDCSFQNHTEAFTKLISMMTTGDGAVIENIKEIDAIGHRVAQGADKFPHS
                     CLLTEQVIQQIADLSELAPLHNPAHIQGIRAAQKVFGADLPQACVFDTSFHHSMPPKA
                     YMFAIPYEYYKKYNIRRYGFHGTSHRYVSKTCAKLMGKPIDQLRIITCHLGNGSSITA
                     VDHGRSIDTTMGMSPTGGLMMGTRSGDLDPSVVTYIAEKTGEHGNEFLRVFNERSGLL
                     GVSGISSDNRDIERAASLGNEQAQLAHDMLCYQIKKYIGGYIAAMGGLDALVFTGGIG
                     ENSDEVRGDVCANMEYLGIKIDEEKNKTKGDIVDLTAEGATVKTFIICTNEELMIARD
                     TKALVEVDAMMHAKKEVAPEE"
     gene            1768..>2183
                     /locus_tag="ACZPFK_11235"
     CDS             1768..>2183
                     /locus_tag="ACZPFK_11235"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR1406930.1"
                     /translation="MERCSWCLGTEEMKRYHDEEWGVPLHDDQKQFEFICLEVLQCGL
                     NWDMMIRKRQIFRRCFEGFDPARVAAYGEAEIERILSTEGMIRSRRKVEAIIGNARCL
                     LALQREHGSFSAWLWGLAGGAPILYAGHQRGGLPSQ"
BASE COUNT          380 a          654 c          679 g          470 t
ORIGIN      
        1 caaaatgggc tattgaggac cctgagaccg ctctgccacc caccggcccg gccttggctt
       61 cggctatgct gaagcccggc ccgccccggg ccgcccaaag cgcgcgggtc ggcggtcgtt
      121 ttcctccaat tcaaaacaaa aacgggaagc cggggagatt gctccccccg cttcccgttt
      181 tatttttatc tgattttatt cggattttac agggcaaaag ccgccctttc ccggggccgc
      241 cgcgcctgtc aaagggcctt tccgcgacga ggatcactcc tcaggcgcga cctctttttt
      301 ggcgtgcatc atggcgtcga cctccaccag cgccttggtg tcgcgggcga tcatcagctc
      361 ttcgttggtg cagatgatga aagtcttgac cgtggcgccc tcggcggtca gatcgacgat
      421 gtcgcccttg gtcttgtttt tctcctcgtc gatcttgatg cccaggtatt ccatattggc
      481 gcagacgtcg ccccgcacct cgtcgctgtt ctcgccgatg ccgccggtga agaccagcgc
      541 gtccaggccg cccatggcgg cgatatagcc gccgatgtac tttttgatct ggtagcagag
      601 catatcgtgg gccagctgcg cctgctcgtt gcccagcgag gccgcccgct cgatatcgcg
      661 gttgtcgctg gagatgcccg acacgcccag caggccgctg cgctcgttaa acacgcgcaa
      721 gaactcgttg ccgtgctcgc cggtcttctc cgcgatatag gtgaccaccg aggggtccag
      781 gtcgccgctg cgggtgccca tcatcaggcc gcccgtgggg gacatgccca tggtggtgtc
      841 gatgctgcgg ccgtggtcca ccgcagtgat ggacgagccg ttgcccagat ggcaggtgat
      901 gatgcgcagc tggtcaatgg gcttgcccat cagcttggcg caggtcttgc tcacatagcg
      961 gtgggaggtg ccgtggaagc cgtagcgccg gatattgtat tttttgtaat actcataggg
     1021 gatggcgaac atgtacgcct tggggggcat cgaatggtgg aacgaggtgt cgaagacgca
     1081 ggcctgcggc agatcggcgc cgaacacctt ctgcgcggcg cggatgccct gaatgtgcgc
     1141 ggggttgtgc agcggtgcca gctcggacag gtcggcgatc tgctggatga cctgctcggt
     1201 cagcaggcag gagtggggga acttgtccgc cccctgggcg acccggtggc cgatggcgtc
     1261 gatctctttg atgttctcga tgacggcgcc gtcgccggtg gtcatcatgg agatcagctt
     1321 ggtgaaagcc tcggtgtggt tctggaacga gcagtccacc ttgatctcat agccgtccgc
     1381 agtcttgtgg gtgatcatgc cgccggggat gccgatcctc tcgcagttgc ccttgcagag
     1441 gaccttttct ccgtccatat cgatcagctg atacttcagg gaactggagc cgcagttgat
     1501 aaccagaatc ttcataaacg ccaaacctcc atttatagtt tcacattcag gatgtcttct
     1561 ctctttaacc aatttattat ataataaaca cagggccttt tcaagaaacc gagccgtttt
     1621 tttgcctttc cagccgcaaa aacagcgctt tttcagcgcg agacggcggt tttgtgtgcg
     1681 gcgggcgcaa aacggcgccg cttttttgcc cggtgaggcg aaaaggcagt caagagccgg
     1741 agagcccggc caaaaggagg gacaggcttg gagcgctgca gttggtgcct tggcaccgag
     1801 gagatgaagc ggtatcacga cgaggagtgg ggcgtgccgc tccacgacga ccaaaagcag
     1861 ttcgaattta tctgtcttga ggttctgcaa tgcggcctca actgggacat gatgatccgc
     1921 aagcggcaga tcttccgccg ctgctttgag ggttttgacc ccgcccgcgt ggccgcctac
     1981 ggcgaggcgg agatcgagcg catcctctcc accgagggga tgatccgctc gcgccgcaag
     2041 gtggaggcca tcatcggcaa cgcccgctgc ctgctggcgc tccagcggga gcacggctct
     2101 ttcagcgcct ggctctgggg cctggccggc ggcgccccca tcctgtatgc gggccaccag
     2161 cgcggcggcc tgccgtccca aaa
//
LOCUS       DBMDYA010000510         2183 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10487_length_2183_cov_1.359750, whole genome shotgun sequence.
ACCESSION   DBMDYA010000510 DBMDYA010000000
VERSION     DBMDYA010000510.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10487_length_2183_cov_1.359750"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            110..1243
                     /locus_tag="ACZPFK_11240"
                     /pseudo
     CDS             110..1243
                     /locus_tag="ACZPFK_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            1505..2098
                     /gene="rdgB"
                     /locus_tag="ACZPFK_11245"
     CDS             1505..2098
                     /gene="rdgB"
                     /locus_tag="ACZPFK_11245"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1406931.1"
                     /translation="MTPTHREAVLEEIRARLKEGAPCRVVATSLIEAGVDVEVEETGT
                     TFEENSLLKARAVMEASGLPAIADDSGLCVDALNGAPGVYSARYGGPGLDDPGRVRLL
                     LENMRGQMPRTCRFVSVITCCFPNGDVLSARGECEGTVAFTPMGEGGFGYDPVFFVPP
                     LKKTFAQLTAEEKNAISHRGKALEAFQVKLREYLEKS"
BASE COUNT          457 a          595 c          731 g          400 t
ORIGIN      
        1 tgaaaaaata gaaaaaataa caaaaatatg ccttaaaaaa taaaaataat agactttttg
       61 acgaggcaaa gttataatgt aaagaagaaa atggaaaaaa gaggatatca tgaagattta
      121 tttggcgcac cgcaccgaga atggccgcga acagacgctg ctggatcatt tgcagggtac
      181 cgccagccgc gcagcacggt tcgctcggag gttcggcgca gaggagctcg cctatcagat
      241 cggcatggcg catgatatcg gcaaatattc tgaggcgttt caacggcatc tgtgtgaggg
      301 cgcgccctcg aaagaccacg ccaccgcggg tggcattgag atcggcaacc tcaatggggt
      361 gccggccgcc ttttgcgtga tggggcatca caccgggctg cctgacggcg gctcttccgc
      421 ggacagcaag gacagcggta ccttgatggg gcggcgaaaa aagccgctgg aggattacgc
      481 tgcctttcat caggagatag agctgaaaaa ggcgtttttc aggtacccgg ccctgctggg
      541 gcaagggcag tttaccgctt ctttctggac gcggatgctg ttttcctgtt tggtggacgc
      601 cgattttctc gacaccgagc agtttatgca gggcacgccc gcggcccgcg gccggggcga
      661 ggggatagag gcactctgcg caaagctgga tcagtatgtg gagggctggt ggaatcccga
      721 ccgccccatt aatcaaaagc gctgccgtat cctgcgggaa tgcatggaaa agggagaaag
      781 cgaggcgccg gggctctttt cgctcaccgt ccccacgggg ggcggcaaaa cggtgagctc
      841 gctggcgttc ggcctctatc acgcccgtgc ccaggggctg gagcgggtca tttacgtcgc
      901 cccctattgc tcgattattg agcagacggc gaaaaaattc cgcgagatcc tggggccgga
      961 aaatgtgctc gagcatcacg ccaatgtgga tctgggcaag gacccggtga aaaaactggc
     1021 ggcggaaaac tgggacatgc cggtcgtcgt caccaccgcc gtccagttct tcgaatccct
     1081 ttttgcaagc aaaacttccc gctgccgcaa gctgcacaac atcgccggca gtgtgattat
     1141 ttttgacgag gcgcagacgc tgccgctgcc ctatctggag ccgtgcctgc gggtgatcgg
     1201 ggagctgacg gtcaactacg gctccagctg tgtgctctgc acggctaccc agccggcgtt
     1261 ggggccgatg ctgcagcaga tctcaccgct gctgcgctgc cgcgagctct gtaccggcgt
     1321 cgaggagctc tatcagtttt ttcgccgcgt gacctttgtg cggtcggggc ggctgacgga
     1381 cgaggcgctg gcgacgcggc tgaatggcga gagccaggtg ctgtgcatcg tggggacccg
     1441 caagcaggcg caggcggtat accatctgct gcagggcgag gggagctacc acctctccac
     1501 cttgatgacc ccaacccaca gagaggcggt tctcgaagag atcagggcgc ggctgaaaga
     1561 gggcgcgccc tgcagggtag tggcgaccag cctgattgag gcgggggtgg atgtggaggt
     1621 ggaggagacc ggcaccacct ttgaagagaa ttccctgctg aaggccaggg cggtcatgga
     1681 ggccagcggt ctgcctgcca tcgccgacga ctccgggctg tgcgtggacg ccctcaacgg
     1741 cgcgccgggg gtgtactccg cccggtacgg agggcctggg ctggacgacc cgggacgggt
     1801 gcgcctgctg ctggagaaca tgcggggcca gatgccccgg acctgccgct ttgtatcggt
     1861 gatcacctgc tgcttcccca acggagacgt gctctctgcc agaggagaat gtgagggcac
     1921 cgtggccttc acccccatgg gggagggagg cttcggctat gacccggtgt tttttgtgcc
     1981 gccgctgaaa aaaacctttg ctcagctgac ggcggaggag aagaacgcca tctcccaccg
     2041 ggggaaggct ctggaggcgt ttcaggtgaa gctgagggag tatctggaga aaagctgacg
     2101 cccggcccca aatgggagcc cagcggagcg ggtcccattt ggagaggacg agcaacgaaa
     2161 tagagcggat gtccgccggg agg
//
LOCUS       DBMDYA010000511         2180 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10506_length_2180_cov_1.072150, whole genome shotgun sequence.
ACCESSION   DBMDYA010000511 DBMDYA010000000
VERSION     DBMDYA010000511.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_2180_cov_1.072150"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZPFK_11250"
     CDS             complement(<1..476)
                     /locus_tag="ACZPFK_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIR1406932.1"
                     /translation="MILRENFWELLLLNFCFCACVLPFLALGLFFLSAGALTPALVCL
                     ALSAPFSGPACCAMARVFCDWQRGRAYAPIRRFWSAYRESWGRGALLGLFYQPLLALA
                     GFACRFYFGSPAGSLLFLLCGWLSLFFSLVLLASSFYGYMMAANLALSLPQVLKNA"
     gene            complement(630..1208)
                     /locus_tag="ACZPFK_11255"
     CDS             complement(630..1208)
                     /locus_tag="ACZPFK_11255"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1406933.1"
                     /translation="MPDPVLLEQKRALRRAVRAAAAGFYPQHGAAYSRRLLGRLLSLP
                     EYQTCLTLFCFVGTGCEADTLPLIRHALQSGRRVAVPLCTGPGRMEARLVPDLSALAS
                     RGAYGIPEPAADAPLLDKEAIDFAVVPCLCCDRDGRRLGHGGGYYDRYLADAAFPWAV
                     VCPQALLAAAVPADPWDVIAPIVATDQAIYRA"
     gene            1491..>2180
                     /locus_tag="ACZPFK_11260"
     CDS             1491..>2180
                     /locus_tag="ACZPFK_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1406934.1"
                     /translation="MIRLIASDIDGTLLQGGERALSEELFGLIRQLHDKGIRFIPASG
                     RQYLNLRSLFAPVAGELIYLCENGALVMEQDRLLSKTALERDAGLELIRQIEGFGPLE
                     ALVSGERTCYVSTRRPDYVEHIRYFVGNHTTALENLSEIAEPFLKISAWCPDGVTPEI
                     ERFFESRWADRFSIAVAGECWLDFTLANKGTGLRAVAERLGVAPGEIAAFGDNFNDLP
                     MMRFAGVSYAME"
BASE COUNT          491 a          624 c          725 g          340 t
ORIGIN      
        1 aaagcgtttt tcagcacctg cggcagcgag agggccagat ttgcggccat catatagcca
       61 taaaaagagg atgccaggag cacaagggaa aaaaagagcg agagccagcc gcagaggaga
      121 aagagcaatg agcccgcagg gctgccaaag taaaagcggc aggcaaagcc ggcgagcgcg
      181 agcagcggct gataaaacag ccccagcagc gccccacgcc cccagctttc gcgataagcg
      241 gaccagaaac gcctgatggg cgcataggcg cggccgcgct gccagtcgca gaacacacgc
      301 gccatcgcgc agcaggcggg gccggagaat ggggcggaaa gcgccagaca gaccagggcg
      361 ggcgttaaag cgcccgccga cagaaaaaac agccccagcg caagaaaggg gagcacacag
      421 gcacaaaagc agaagttgag cagcaaaagc tcccaaaagt tttcccgcag gatcacagcc
      481 gaagaaaccc ggccctgcga ctggccggga catggagggc ctgcgggcgc cgcgcggccc
      541 ttggaatgaa agaaaggcat aaaactcctt tgcatgaggg aatggacgaa agaaaacgga
      601 aaaaggagaa ggggtgagaa aaggggagat caggcgcggt aaatcgcttg gtcggtcgcc
      661 acgatgggcg cgatgacgtc ccaggggtcc gcgggcacgg cggcggcgag cagcgcttgg
      721 gggcagacga ccgcccaggg gaaagcggcg tccgcgagat agcggtcgta gtagccgccc
      781 ccgtggccga gcctgcgccc atcccggtcg cagcaaaggc agggcaccac ggcaaagtcg
      841 atcgcctcct tgtccagcag cggggcgtcc gccgctggct ccgggatgcc gtacgcccca
      901 cgggaggcga gcgcgctgag atccgggaca aggcgcgctt ccatccgtcc cggcccggtg
      961 cacaggggga ccgccacccg gcgtccgctt tgcagcgcgt gccggatcag gggcagcgtg
     1021 tccgcctcgc agcccgtacc cacaaagcag aacagggtga ggcaggtctg atattcgggc
     1081 agggagagca gccgccccaa aagccggcgg gaataggcgg cgccgtgctg tggatagaag
     1141 cccgcggcgg cggcgcggac cgcgcgtcgc agcgcccttt tctgctctaa aagaacgggg
     1201 tccggcatga caaacttcct ctctgccgtt ttcaaaagca cggctttgtg atattatggg
     1261 gctgttgaca aagtcgctcc ttgaaaacat ggaccctttg ttccggcata cttcacaaaa
     1321 atacgcgtga agccagcaaa gcttgcctgc gtatttcgtt ttatccgccg aaaaaatcgc
     1381 ctgcgttttc tgcggctttt ggtttgtcaa cagtcccatt atggaccgta gtgtttatct
     1441 tagcacagac aaagggaaag gaaaagggaa aatttttctc agggatgaaa atgatccgat
     1501 tgatcgcgag cgacattgac gggacgctgc tgcagggcgg agaacgcgcc ctcagcgaag
     1561 agctgttcgg cctgatccgg cagcttcatg acaagggaat ccgctttatc ccggccagcg
     1621 gccggcaata tctcaacctg cgcagcctgt ttgcgccggt ggcgggcgag ctgatttatc
     1681 tgtgcgaaaa cggggcgttg gtgatggaac aggaccgcct tttgagcaag acggcgctgg
     1741 agcgcgacgc cgggctggag ctcatccgcc agatcgaggg atttgggccg ctggaggcgc
     1801 tggtcagcgg cgagcgcacc tgttatgtct cgacgcgcag gcccgactat gtggaacata
     1861 tccgctattt tgtgggcaac cacaccacgg cgctggagaa tctctccgag atagcggagc
     1921 cgtttctcaa aatttcagcc tggtgcccgg acggggtcac cccggagatc gagcgctttt
     1981 ttgagagccg ctgggcggac cgcttcagca ttgcggtggc gggggagtgc tggctggatt
     2041 ttacccttgc caacaagggc accggcctgc gggccgtcgc cgagcgcctg ggggtcgccc
     2101 ccggggaaat cgcggccttt ggggacaatt tcaacgacct gcccatgatg cggttcgccg
     2161 gcgtctccta tgcgatggaa
//
LOCUS       DBMDYA010000512         2173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10540_length_2173_cov_1.404440, whole genome shotgun sequence.
ACCESSION   DBMDYA010000512 DBMDYA010000000
VERSION     DBMDYA010000512.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10540_length_2173_cov_1.404440"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="ACZPFK_11265"
     CDS             complement(<1..547)
                     /locus_tag="ACZPFK_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1406935.1"
                     /translation="MKAKKQSEKKKRRFSLWKLFENSQFNVVLSIIIAVLAWLIVSMY
                     VKPETEGTVSNIPVKLDYSAQTLQAMGLDVVTEGDAPTASVRVTGDRAVLGGLSAEDI
                     TVYPRLSNVTEAGQYDLELVAVKNDSSATYTIKSVQPAKITVRFATLTQKKFVVETDT
                     SAVKPQEGYILTNAYATPGEVT"
     gene            complement(525..1481)
                     /gene="cdaA"
                     /locus_tag="ACZPFK_11270"
     CDS             complement(525..1481)
                     /gene="cdaA"
                     /locus_tag="ACZPFK_11270"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1406936.1"
                     /translation="MGLAESWSAFIRAMGTGFWGFLESVGNVLRDFKPADLLDIAVVA
                     FLTYELIVLVRQTRAAQLVKGILMLFVASFLSDLWAMRTLSYVLQKILQFGVLALVVV
                     FQPEIRRALEQVGRTNIFAISIFKGKTPDEQMRQRWKTAIATVCDAAEQMSDSRTGAL
                     IVFERRSNLSEIIKTGTVLNADINVEAIGTIFYEGTPLHDGAVVVRDARIKAAGCVLP
                     LSANQDISKDMGTRHRAALGMSENSDALCVVVSEETGIISMAKNGVLIRRLDRQNLYN
                     LLQMELVPDPPAEEERRTKRVLNRLRGKKAKEVGADESEKAE"
     gene            complement(1648..2124)
                     /gene="ispF"
                     /locus_tag="ACZPFK_11275"
     CDS             complement(1648..2124)
                     /gene="ispF"
                     /locus_tag="ACZPFK_11275"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1406937.1"
                     /translation="MRVGHGYDVHRLVAGRKLILGGVEIPWEKGLLGHSDADVLTHAV
                     MDALLGAAALGDIGRHFPDRDPAYAGANSLELLRRVAGLLREAGWAAENIDATLLCQA
                     PKLAPHIPEMRRRLAEAAGLPEASVSVKATTEEGLGFTGAGEGIAAHAVCLLRAGA"
BASE COUNT          356 a          764 c          649 g          404 t
ORIGIN      
        1 gggtgacctc ccccggcgtg gcgtaggcgt tggtgagaat atatccctcc tgcggcttca
       61 ccgcgctggt gtcggtctcc accacaaatt ttttctgggt gagggtggcg aaccgcaccg
      121 tgatcttggc gggctgcacc gacttgatgg tgtaggtggc cgagctgtcg ttcttcaccg
      181 ccaccagctc caggtcgtac tgccccgcct cggtgacatt gctcagcctg gggtagacgg
      241 tgatgtcctc cgcgctcagg ccgcccagca cggcgcggtc gccggtgacc cggacgctgg
      301 ccgtgggcgc gtccccctcg gtcaccacgt ccagccccat ggcctgcagg gtctgggcgc
      361 tgtaatcgag cttcaccggg atattggaca ccgtgccctc ggtctccggc ttgacgtaca
      421 tcgagacgat cagccaggcc agcacggcga tgatgatgga gagcaccacg ttgaactggc
      481 tgttctcaaa cagcttccag aggctgaagc ggcgcttttt cttctcactc tgctttttcg
      541 ctttcatcgg cgcctacctc cttcgctttt ttgccgcgca gccggttcag cacgcgcttt
      601 gtgcgccgct cctcctccgc gggcgggtcg ggcaccagct ccatctgaag caggttgtac
      661 aggttctgcc ggtccagccg gcggatcagc acgccgtttt tagccatcga gatgatgccg
      721 gtctcctccg acaccaccac gcaaagcgcg tcggagtttt cactcatgcc cagcgccgcc
      781 cggtggcggg tgcccatatc cttgctgatg tcctggttgg cagacagcgg cagcacgcag
      841 cccgcagcct tgatgcgcgc gtcccgcacc accaccgcgc cgtcgtgcag cggcgtgccc
      901 tcgtaaaaga tggtgccgat ggcctccaca ttgatgtccg cgttgagcac cgtgccggtc
      961 ttgatgatct cgctcaggtt ggagcggcgc tcaaacacga tcagcgcgcc ggtgcgtgaa
     1021 tcgctcatct gctcggcggc gtcgcagacc gtggcgatgg ccgtcttcca gcgctggcgc
     1081 atctgctcgt ccggcgtttt gcccttaaag atcgagatcg caaagatgtt ggtgcggccc
     1141 acctgctcta aggcgcggcg gatctcgggc tgaaagacca ccaccagcgc cagcacgccg
     1201 aactgcaaaa tcttctgcag cacatagctg agggtgcgca tggcccacag gtcggagagg
     1261 aacgacgcca cgaacagcat caggatgccc ttgaccagct gcgcggcgcg ggtctgccgc
     1321 accagcacga tcagctcata ggtcaaaaag gcgaccaccg cgatgtccag caggtcggcg
     1381 ggcttaaaat cccgcaggac gttgcccacg ctctccaaaa agccccaaaa gccggtgccc
     1441 atcgctcgga taaaagcact ccaggattct gccaggccca tattatagcc tccgttccca
     1501 agaaagccgg gggtttgcct cccagcatct attttactct atcttttcaa gtataccaca
     1561 acacgggcct tttctcaatg caattgcggg ccgcggcggc cgctccgccg ctgtttcccg
     1621 ccccgcacac acaaaggggc gagagggtca ggcccccgcc cgcagcagac agacggcgtg
     1681 ggctgcgatg ccctcgcccg cgccggtaaa gcccaggccc tcctcggtgg tggcctttac
     1741 cgacaccgac gcctccggca ggccggcggc ctcggcgagg cgccgccgca tctccgggat
     1801 atgcggggcc agcttggggg cttggcaaag gagggtcgcg tcgatattct ccgccgccca
     1861 gcccgcctcc cgcagcagcc cggccacccg gcgcagcaat tccaggctgt tcgccccggc
     1921 gtaggccggg tcccggtccg ggaagtgccg cccgatatcc cccagcgccg ccgcgcccaa
     1981 cagcgcgtcc atgaccgcat gggtcagcac atccgcgtcc gagtggccca gcaggccctt
     2041 ttcccaaggg atctccaccc cgcccaagat cagcttgcgg cccgccacca gacggtggac
     2101 gtcatatccg tgcccgacgc gcatcgccgg cgcctccttt ttttcgtacg gcgccaggtc
     2161 ctcccgcgtc gtg
//
LOCUS       DBMDYA010000513         2172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10543_length_2172_cov_1.519556, whole genome shotgun sequence.
ACCESSION   DBMDYA010000513 DBMDYA010000000
VERSION     DBMDYA010000513.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10543_length_2172_cov_1.519556"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="ACZPFK_11280"
     CDS             <1..240
                     /locus_tag="ACZPFK_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406938.1"
                     /translation="LAGEEELEAVFDMITSVPARNCLLEGYGIREGQQADLVILDADS
                     PAEAVRRGGGVLLQYAGGRYILKDHGDGENKKALG"
     gene            complement(640..1578)
                     /locus_tag="ACZPFK_11285"
     CDS             complement(640..1578)
                     /locus_tag="ACZPFK_11285"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406939.1"
                     /translation="MNKRPVVIDCDPGVDDTLALCLAASRPELDIRAINPVAGNVGYE
                     STSQNALRLADLIGVDCRVGRGAEEPLLAPCRTAGSIHGQGGLGGYQLPEAGRDFDPD
                     YAWDVLYQEAVRAGGELELVTLGPLTNVAIALKRHPDLAGLIKKITMMAGTAGQGNTN
                     VYAEFNVWVDPHACDIVFRSGIPIAMCGLDGNDSCRLDTGEMAEVFDRPGRLSEVISH
                     VAHFIHQRNTEQWHLPGSTINDLVTMACFIEPSMAVYEPCYTVCETRSTISAGQTVVD
                     LYHVSGKEPNVDVLLRADKERYKALLQGMMDFYAAQ"
     gene            complement(1594..>2172)
                     /locus_tag="ACZPFK_11290"
     CDS             complement(1594..>2172)
                     /locus_tag="ACZPFK_11290"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406940.1"
                     /translation="IAVGPLTNIALLLKAYPHLKARIERIAIMGGGAFRGNASPAAEF
                     NIYVDPEAARMVFKAGIPMIMCGLDVTMKAYATGEDIDAFAALGTRAGKFCADIFRFY
                     YDFHMSGVRGKLPGCAVHDAVTVMALTHPELMRGEDAYVEVDIDGEHTWGCTASDFRE
                     RNCSGEKNARVILDIDREAFIQQLLKAAASFS"
BASE COUNT          383 a          638 c          749 g          402 t
ORIGIN      
        1 ctggcggggg aagaagaatt ggaggctgtt ttcgacatga ttacttccgt cccggcgcgc
       61 aactgcctgt tggaggggta tggtatccgt gagggccagc aggccgacct ggtgatcctg
      121 gatgcggatt cgcctgcgga ggcggtgcgc cgcgggggcg gggtgctgct gcaatatgcg
      181 ggcggccggt acattttgaa agaccacggc gacggggaga acaagaaagc gctgggctga
      241 ggcgtattga gacaaaaaac cgcttgtttt gggcgcgttg aggtgagagg acactaattt
      301 tgaatcgacg gtcctgacgt ttcgcgtatg taaaaaggcc ggcaatccgc agagattgcc
      361 ggcctttgtt ctgctgtgct tccctgctgc ccaccgagcc tctccacgga gagagggccg
      421 ccagacgcgc tgccgttggc ggagtgcggg gccaaagacg cacctcgctc gccgccctcg
      481 gtcgggagga cgccccccgc tttgcgggcg ccaaagaata caaagcgctg agatagaaaa
      541 agcacgcggg aaaaatcccc gcgtgctttt ttatctttgg ccctattctt ggccgggcgg
      601 tccaaaacag agctccccgt ccaaaaaaaa ttctgtgcgt tattgggcgg cgtaaaaatc
      661 catcatgccc tggagcagcg ctttgtagcg ctccttgtcg gcgcgcagca gcacatccac
      721 gttgggctct ttgccgctca cgtggtacag gtctaccacc gtctggcccg ccgagatggt
      781 gctgcgggtc tcgcagacgg tataacaggg ctcatagacc gccatggagg gttcgataaa
      841 gcaggccatg gtcaccaggt cgttgatggt ggagccgggc agatgccact gctcggtgtt
      901 gcgctgatgg atgaaatgcg ccacatggct gatgacctcg gagaggcggc cgggccggtc
      961 gaagacctcg gccatctcgc cggtgtccag gcggcagctg tcgttgccgt ccagcccgca
     1021 catggcgatg gggatgccgg accggaagac gatgtcgcag gcatgggggt ccacccagac
     1081 gttgaactcg gcgtagacgt tggtgttgcc ctgcccggcg gtgccggcca tcatggtgat
     1141 ttttttgatg aggccggcga gatccgggtg ccgcttgagg gcgatggcca cgttggtcag
     1201 cgggcccagg gtgaccagct ccagctcgcc gccggcgcgc acggcctcct gataaagcac
     1261 gtcccaggcg tagtcggggt caaaatcgcg tccggcctca ggcagctgat agccgcccag
     1321 gccgccctgg ccgtggatgg agccggcggt gcggcagggg gcgagcaggg gctcctcagc
     1381 gccccggccc acgcggcagt ccacgccgat caggtcggcc aggcgcaggg cgttctgcga
     1441 ggtgctctca taccccacat tgcccgccac cgggttgatg gcgcggatat ccagctccgg
     1501 ccggctggcg gccaggcaga gggccagggt gtcgtccacc ccgggatcac agtcgatgac
     1561 gacgggtcgt ttgttcatga tttggttcct cctttacgaa aagctggcgg ccgctttcag
     1621 cagctgttgg atgaaagcct cccggtcgat gtccaggatc acgcgggcgt ttttctcacc
     1681 cgagcagttc cgctcacgga agtcggaggc ggtgcagccc caggtgtgct cgccgtcgat
     1741 gtccacctcc acataggcgt cctccccccg catcagctcg gggtgggtga gggccatcac
     1801 ggtcacggcg tcgtggacgg cgcagccggg cagcttgccg cgcacgccgc tcatatggaa
     1861 atcatagtaa aagcggaaga tgtcggcgca gaatttgccc gcccgggtgc ccagggcggc
     1921 aaaggcgtcg atgtcctcgc ctgtggcata ggctttcatg gtcacgtcca ggccgcacat
     1981 gatcatgggg atgccggcct tgaagaccat gcgggccgcc tcggggtcca catagatgtt
     2041 gaactcggcg gcgggggagg cgttgccgcg gaaggcgccg ccgcccatga tggcgatccg
     2101 ctcgatgcgg gccttgagat ggggataggc tttcagcagc agggcaatat tggtgagcgg
     2161 acccacggcg at
//
LOCUS       DBMDYA010000514         2172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10545_length_2172_cov_1.253018, whole genome shotgun sequence.
ACCESSION   DBMDYA010000514 DBMDYA010000000
VERSION     DBMDYA010000514.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_2172_cov_1.253018"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="ACZPFK_11295"
     CDS             <1..463
                     /locus_tag="ACZPFK_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1406941.1"
                     /translation="DRLGDIPVGSAVRFRPVAYDDREYYAVVEDTPANIRRQLTTTGY
                     RSVADIYATVEAADEYLENGLTVTSTVLIADRQQLMTIPYKAIDQDEKGEYVYRIENG
                     RAVKNYIVTGLELEDTVEVQSGLDLGASIVEDCSAVRSAGHLVRLKGEKAA"
     gene            460..1650
                     /locus_tag="ACZPFK_11300"
     CDS             460..1650
                     /locus_tag="ACZPFK_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406942.1"
                     /translation="MKKKLGLLAAEFKSEFAPYRTAALSIAIAITAIVIVSVISGVGS
                     SLIDTELSGLGLDGVALSVAEKTDRNQMTAALYQQLKEVSGVKDSTPIVMDSGAYELG
                     AKTGNAFFWGIAPKAENIVTLELLHGQMIGSEEVLGARKVCIVDEKIARDAYQRTNIV
                     GKTIRLTINGVTDGYTVIGVVKAGSSLLNGLTGNVMPNFIYLPYSTLSFFSAKEGFDQ
                     ILFTARQPDGLDEVKEEITAALRGITQGGETDFVLNDLAAQRDSIDRIVLVASGALAL
                     IAGISLVVGGLAVSSSVGTAVHTRRREIGIKKSLGAPPSVIVKEFLAQILFTSLGAAL
                     TGTLFSGLGCWLLLTVFGLPLLLDLPLLLGGIFATIGLSILFGVFPALRAAKMKPVDA
                     LRGE"
     gene            1657..>2172
                     /locus_tag="ACZPFK_11305"
     CDS             1657..>2172
                     /locus_tag="ACZPFK_11305"
                     /inference="COORDINATES: protein motif:HMM:NF045454.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HIR1406943.1"
                     /translation="MKFSKPNLPQGAACLCVVSGGNKQVIRALEQRGVTCYITRPSRA
                     LSAPVASHADMLALSFKPGALILAADQRELHARLQKNGVEAVRFDGLKPGYPTETALN
                     AAVIGKWLIGGETALSTLKSFETNKLEVLSVRQGYARCSVVPVAENALMTADKGIAAA
                     ARGAGLEVLELQ"
BASE COUNT          424 a          646 c          708 g          394 t
ORIGIN      
        1 ggaccggctc ggggatatcc cggtgggcag cgccgtgcgc tttcggcccg tcgcctatga
       61 cgatcgcgag tactatgcgg tcgtggagga cacccccgca aacatccgcc gccagctcac
      121 caccaccggc taccgcagcg tcgcggacat ctatgccaca gtggaggcag ccgatgaata
      181 tctggaaaac ggccttacgg tcacctccac cgtgctgatc gcagaccggc agcagctgat
      241 gacaatcccc tataaggcaa tcgatcagga cgaaaaaggc gaatacgtct atcggatcga
      301 gaacggacgc gccgtcaaga actatatcgt caccgggctg gagctggagg acacggtgga
      361 ggtgcaaagc ggcctggacc tcggggcgtc catcgtggag gattgctcgg cggtgcgcag
      421 cgccgggcac ctggtgcggc tgaaagggga gaaagcggct tgaagaaaaa acttggcctg
      481 ctcgcggcgg agttcaaaag cgaattcgcc ccctatcgca ccgcggcgct ctccatcgcc
      541 atcgccatca cggcgatcgt catcgtctcg gtgatctccg gcgtcggctc ctccctgatc
      601 gacaccgagc tttccggcct ggggctggac ggggtcgcgc tctcggtggc ggaaaagacc
      661 gaccgcaacc agatgacggc ggcgctctat cagcagctga aagaggtctc cggcgtcaag
      721 gacagcaccc ccatcgtcat ggacagcggc gcctatgagc tgggtgccaa gacgggaaac
      781 gcctttttct gggggatcgc tcccaaagcg gaaaacattg taacgctgga gcttttgcac
      841 ggccagatga tcggcagcga ggaggttctg ggggcccgaa aggtatgcat cgtggacgaa
      901 aaaatcgccc gcgacgcata ccagcggacc aatatcgtcg gcaagacgat ccggctgacc
      961 atcaacgggg tgacggacgg ctatacggtg atcggcgtcg tcaaggccgg cagcagcctg
     1021 ctcaacggcc tcaccggcaa cgtgatgccg aattttatct atttgcccta ctcaacgctg
     1081 tcgtttttca gcgcaaagga gggctttgac cagatccttt tcaccgcgcg ccagccggat
     1141 gggctggatg aggtaaaaga ggagatcacc gccgccctgc gcggcatcac acagggcggg
     1201 gagacagatt ttgtgctcaa tgacctggcg gcccagcggg acagcatcga ccgcatcgtc
     1261 ctggtggcct ctggggcgct ggccctgatc gcaggcatct ccctggtggt gggcgggctg
     1321 gcggtgagca gctcggtggg cacagcggta cacacccggc ggcgggagat cggcatcaaa
     1381 aaaagcctgg gggccccgcc gtcggtgatc gtaaaagagt ttttggcaca gatcctcttc
     1441 acctccctcg gggcggccct gacgggaacc ctgttcagcg gcctgggctg ctggctgctg
     1501 ctcacggtgt tcgggctgcc gctgttattg gatctgcctt tgcttctcgg cggtattttt
     1561 gctacaatag ggctgtcgat cctgttcggc gtttttcccg cgctgcgcgc ggccaaaatg
     1621 aaacccgtgg atgcattgag aggggagtag aggcggatga aattcagcaa gcccaatctg
     1681 ccgcagggcg ccgcgtgtct gtgcgtggtc tccggcggca acaagcaggt gatccgagcg
     1741 ctggagcagc gcggcgtcac ctgttacata acgcggccgt cacgggcgct ctcggcgccg
     1801 gtggcgagcc acgcggatat gttggcgctt tctttcaagc cgggagcctt gatcttggcc
     1861 gccgaccagc gcgagctgca cgcccgcctg cagaaaaacg gcgtggaagc ggtgcggttt
     1921 gatgggctca agcccggata tcccaccgag acagccctca acgcggcggt gatcggcaag
     1981 tggttgatcg ggggcgagac cgctctttcc accctgaaaa gtttcgaaac aaacaaattg
     2041 gaagtgctgt ccgtccgcca gggctatgcg cgctgttccg tcgtgccggt tgccgaaaac
     2101 gctttgatga ccgcggataa aggcattgcc gccgcggccc gcggtgccgg gcttgaagtt
     2161 ctggaactgc ag
//
LOCUS       DBMDYA010000515         2168 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10564_length_2168_cov_2.060958, whole genome shotgun sequence.
ACCESSION   DBMDYA010000515 DBMDYA010000000
VERSION     DBMDYA010000515.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_2168_cov_2.060958"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="glgB"
                     /locus_tag="ACZPFK_11310"
     CDS             complement(<1..1817)
                     /gene="glgB"
                     /locus_tag="ACZPFK_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1406944.1"
                     /translation="MELAKERADFLAGRSFTAWRWLGGRFAPGAAGAVFRVYAPGARG
                     VGLIAEWNGWQPAPMAREPDGFWVLEDPAAQEGGLYKYRILGADGRTVDRADPFALCT
                     ELRPGCASRLARLAPTRARTGWRPAPGRGYDRPMSIYELHAGSWRRRADGGWLFYGEL
                     AQPLIPYLQRHGFTHVELLPLAEHPFDGSWGYQVSGYFSATSRYGDPRGLQYLIDELH
                     RAGIGVLMDFVPVHFVADDFGLHRFDGTPLYEYEHPDIAQSAWGSCNFDYYKPVVRSF
                     LQSAADFWLSFYHCDGLRMDAVSNALYWQGDPSRGVNVGGVEFLRGLNSGLHRRHPDA
                     LLMAEDSSTFLKVTAPVEYDGLGFDYKWDMGWMHDTLDFFAAPPDARGRLRGQLAFSM
                     QYFAGELYLLPLSHDEVVHGKKTILDKLPGGYEEKFAQCRALFAYMFCHPGKKLNFMG
                     NELGQFREWDEGRELDWNLLDYPMHAAFDRCFAALGELYLAHPALWDGEYDLKNWALL
                     DTGDPEVFAFSRTGGGERLLFALNLSGALKQEARVAWRDRAPLQELLNTDEQRFGGAG
                     RANGPLAPAYGGVVLQLAPFAAAIFACGGRAADAALPQAR"
BASE COUNT          382 a          764 c          713 g          309 t
ORIGIN      
        1 cgcgcctgtg ggagagcggc gtcggcggcc cgcccgccgc aggcaaagat cgcggccgca
       61 aagggggcca gctgcagcac cacgccgccg taggccgggg cgagcgggcc gttggcacgc
      121 ccggcgcccc caaagcgctg ctcgtcggtg ttcagcagct cctgcagcgg cgcgcggtcc
      181 cgccaggcca cccgggcttc ctgcttcagc gcgccggaaa ggttcagcgc gaacagcagc
      241 cgctcgccgc cgcccgtgcg cgaaaaggcg aatacctccg ggtcgccggt gtccagcagc
      301 gcccagtttt tgaggtcgta ctccccgtcc caaagggccg ggtgcgcgag atagagctcg
      361 cccagcgcgg caaagcagcg gtcaaaggcg gcgtgcatcg ggtagtccag caggttccag
      421 tccagctcgc ggccctcgtc ccactcgcgg aactgcccca gctcgttgcc catgaaattg
      481 agctttttgc ccgggtggca gaacatgtag gcgaacaggg cgcggcactg ggcgaatttt
      541 tcctcatagc cgccgggcag cttgtccagg atcgtctttt tgccgtgcac cacctcgtcg
      601 tgggagaggg gcagcaggta gagctcgccg gcgaaatact gcatcgaaaa ggccagctgg
      661 ccccgcagcc gcccgcgggc gtccggcggc gcggcaaaaa agtccagcgt gtcgtgcatc
      721 cagcccatgt cccacttgta gtcgaacccc aggccgtcgt actccaccgg ggcggtcacc
      781 ttgagaaagg tggaggagtc ctccgccatc agcagcgcgt ccgggtggcg gcggtgcagg
      841 ccgctgttga gcccgcgcag aaactccacc ccgcccacgt tcaccccccg cgaggggtcg
      901 ccctgccagt agagcgcgtt ggagaccgcg tccatccgca gcccgtcgca gtggtaaaag
      961 gagagccaga aatccgccgc ggactgcaaa aacgagcgca ccaccggctt gtaatagtcg
     1021 aaattgcagc tgccccaggc gctctgggcg atgtccgggt gctcatattc ataaagcggc
     1081 gtgccgtcga agcggtgcag gccgaaatcg tccgccacaa agtgcacggg cacaaaatcc
     1141 atcagcaccc cgatgcccgc ccggtgcagc tcgtcgatca ggtactgcag gccgcggggg
     1201 tcgccgtagc ggctggtggc gctgaaatag ccgctcacct ggtaccccca gctgccgtca
     1261 aaggggtgct cggccagcgg cagcagctcc acatgggtga agccgtgccg ctgcagatag
     1321 gggatcagcg gctgcgccag ctcgccgtag aacagccagc cgccgtccgc acgccgccgc
     1381 caggagccgg cgtgcagctc atagatgctc atgggccggt cgtacccgcg ccccggcgcg
     1441 ggacgccagc cggtcctggc acgggtgggg gcaagccgcg ccaggcgcga ggcgcagccc
     1501 ggccgcagct cggtgcacag cgcaaagggg tccgcccggt ccaccgtgcg gccgtccgcc
     1561 cccagaatgc ggtatttgta cagcccgccc tcctgggcgg cggggtcctc cagcacccaa
     1621 aagccgtccg gctcccgcgc catcggggcc ggctgccagc cgttccactc cgcgatcagc
     1681 cccaccccgc gcgcgccggg cgcatagacg cgaaagaccg cgcccgccgc ccccggcgca
     1741 aagcgcccgc ccagccagcg ccaggccgta aagctgcgcc cggccaaaaa atccgcccgc
     1801 tcctttgcca actccatccc catgaccgcc cctttccacc gccgggccgc cccgacacgg
     1861 ttttgcttgt ccaccccaat tgtatcattt ttctgccgcc gggtgctatt ttttgcgaca
     1921 gagacataaa tctgtacaaa tgacacacgg ggcggcgcgc cgcccgcgag agtaaatttg
     1981 gcagggggat ctgccgggga ggcctgagat gtttgtgttt acgatgaaca aaaacggact
     2041 gaaaaaagcc gctgtcgtgg tcgggtgcgc cgcgctgctg gtgctgtccg cagtggtggg
     2101 gagcggcgcg gtgaaaaagg gggacgccaa gaccaccgcg ctgaccggga cgtccgacac
     2161 cgcaaagc
//
LOCUS       DBMDYA010000516         2160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10604_length_2160_cov_1.629917, whole genome shotgun sequence.
ACCESSION   DBMDYA010000516 DBMDYA010000000
VERSION     DBMDYA010000516.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10604_length_2160_cov_1.629917"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            26..101
                     /locus_tag="ACZPFK_11315"
     tRNA            26..101
                     /locus_tag="ACZPFK_11315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Lys,seq:ctt)
     gene            134..209
                     /locus_tag="ACZPFK_11320"
     tRNA            134..209
                     /locus_tag="ACZPFK_11320"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:167..169,aa:Asn,seq:gtt)
     gene            complement(439..825)
                     /locus_tag="ACZPFK_11325"
     CDS             complement(439..825)
                     /locus_tag="ACZPFK_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1406945.1"
                     /translation="MDIFDKISARLTDLGMTQADLARATGISTGLISQWKKRTQKPSA
                     EKLKKVADALGLSVDELLSAEAPPAAPREQASAIAQTREERKLLLLARRASELPDAEY
                     KKLLKNFEETIDIYLEARGIAPRGKL"
     gene            1176..>2160
                     /locus_tag="ACZPFK_11330"
     CDS             1176..>2160
                     /locus_tag="ACZPFK_11330"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1406946.1"
                     /translation="MSDKKKIILDVDTGTDDAVAIMTAYLDPQIDLIGVCTVAGNKPL
                     LNTTENTLRVKDLLKAGFPVYRGCSVAMVSTLLPNRHGGYQGQRTQVEGMEKPIEIHT
                     DYLELPPSVSKVEPQHAVWYYIDTLMKSEGDITLVPVGPLTNVAMAMRLEPEICKKIK
                     EIVLMGGGRNRTNTSMAAEFNIWADPEAAQIVMESGCPIRIVPLDATHHACINADETR
                     YLRTLHNPVADAVADCVDHRILAYSHLQPMERLDSAPVHDALAVCAVVHPEVLKNLLF
                     TRVDVDISGGFADGQTIVDPRTYTDRPRNAYFAMDADRDLFAGWMMDCIRRF"
BASE COUNT          448 a          647 c          651 g          414 t
ORIGIN      
        1 gctgcgccgc ccgcgcagct tccatgcacc tctagctcag ttggtagagc agctgactct
       61 taatcagcgg gcccagggtt cgagtccctg gaggtgcacc aggcgaactc ctgccggcag
      121 gcagttctta tattcctcct tagctcagtc ggtagagcat gcggctgtta accgcagggt
      181 cgttggttcg agcccaacag ggggagccag aaacaaaccg catcagaccg ccgtttttcg
      241 gctgtttggt gcggtttttc ttttgcaaat aacccacttt ttattttact ttttcgcgct
      301 tttctttttt gaaacaaagg tgcaggaacg atatcttcgc gccgcggtgc ccgctttctc
      361 ctctctgatt tcatggccgc aggctccgca cctgccgcgg gggcagctcg cagaaaaaag
      421 ggaaaaaaga ggaggtcttt acaattttcc tctcggcgcg atccccctgg cctccaggta
      481 gatgtcgatg gtctcctcaa aattcttcaa aagctttttg tattcggcgt cgggcagctc
      541 ggaggcccgg cgggccaaca gaagcagctt gcgctcttcg cgggtctggg caatggcgga
      601 ggcctgctcc cgcggcgcgg cagggggcgc ttccgcgctc agcagttcgt cgacagagag
      661 ccccaaggca tcggcgacct ttttcagctt ttcggccgag ggcttttggg tgcgcttttt
      721 ccactgggag atcaggccgg tggaaatgcc ggtggcgcgc gccagatcgg cctgcgtcat
      781 tcccaaatcc gtcagccggg cgctgatctt gtcaaagatg tccatcgttc atcctctcgc
      841 tttaaaaaat tttgcagatg tttagcactg aaacttagca aactaagtat acgtcaaaaa
      901 cggaaaaaac acaagcaaaa acgcgaaagg acgcccgatc catccctttc cacagcagcg
      961 ggaaaaccgg acaagaggag aggggagaaa cagtcctccc aagccagccg cactgcctgc
     1021 cgggcacacg gcgaaaggtg aaaagtgcgg ctttcgcctt gacccggggc atgggacagg
     1081 gtatgatggt aaatatcgct ccctctccgc cgggatgaaa tgaggcggca cccagggccg
     1141 ggcttgatcc ggctggagtt taaggagact ctttcatgtc tgacaagaaa aaaatcatcc
     1201 tcgacgtgga caccggcacc gatgacgccg tggccatcat gaccgcctat ctcgacccgc
     1261 agatcgacct gatcggcgtg tgcacggtgg ccggcaacaa gccgctgctc aacaccaccg
     1321 agaacaccct gcgggtgaaa gacctgctga aagcgggttt tcccgtgtac cgggggtgca
     1381 gcgtggcgat ggtcagcacc ctgctgccaa accgccacgg cggctatcag ggtcagcgca
     1441 cacaggtgga ggggatggaa aagcccatcg agatccacac ggactatctg gagctgccgc
     1501 cctcggtgag caaggtggaa ccccagcacg ccgtctggta ctacattgat accctgatga
     1561 aaagcgaggg ggatatcacg ctggtgccgg tggggccgct gaccaacgtg gcgatggcga
     1621 tgcggctgga gccggagatc tgcaaaaaga tcaaggagat cgtgctgatg ggcggcggca
     1681 gaaaccgcac caacacctcg atggcggccg agttcaacat ctgggcggac ccggaggcgg
     1741 cacagatcgt gatggagagc ggctgtccca tccgcatcgt gccgctggac gccacccacc
     1801 acgcctgcat caacgccgac gagacccggt acctgcgcac cctgcacaac ccggtggcgg
     1861 acgcggtggc ggactgtgtg gaccaccgca ttttggccta cagccacctg cagcccatgg
     1921 agcgcctgga ttcggcgccg gtacacgacg cgctggcggt ctgcgcggtg gtccaccccg
     1981 aggtgctcaa aaacctgctt ttcacgcggg tggatgtgga tatcagcggc ggctttgccg
     2041 acggccagac catcgtggac ccgcgcacct acaccgaccg gccgcgcaac gcctatttcg
     2101 ccatggacgc ggaccgggac ctcttcgcgg gctggatgat ggactgcatc cggcgctttc
//
LOCUS       DBMDYA010000517         2158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10616_length_2158_cov_1.619835, whole genome shotgun sequence.
ACCESSION   DBMDYA010000517 DBMDYA010000000
VERSION     DBMDYA010000517.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10616_length_2158_cov_1.619835"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(85..456)
                     /locus_tag="ACZPFK_11335"
     CDS             complement(85..456)
                     /locus_tag="ACZPFK_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1406947.1"
                     /translation="MKSLFEQMGGTYRQEGDYLIPNLYLPDEPDYQIGKYGRMRRSYL
                     KAHRPVLYANYLTSGTLHQHLAEIDQACNERMEIIVSAMAKQEGVTEALKAADQMEWV
                     RRMNNIRSRAEEIVLTELVYE"
     gene            698..1093
                     /gene="rbsD"
                     /locus_tag="ACZPFK_11340"
     CDS             698..1093
                     /gene="rbsD"
                     /locus_tag="ACZPFK_11340"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIR1406948.1"
                     /translation="MKRDRLLNREIISEVAALGHTEYLCIADCGLPVPKGVKVIDVSI
                     TAGKPSFLDVLDAVDEELVVESIILAEEIDDKNQPLAGEMNKRFEGKPICKVPHEEFK
                     KLTEKAKCIIRTGENSSYANVILVGGVNF"
     gene            1169..>2158
                     /locus_tag="ACZPFK_11345"
     CDS             1169..>2158
                     /locus_tag="ACZPFK_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1406949.1"
                     /translation="MKKFLSLVLAAAMVFAMTACGGNDTSSTPAAGGSSAAASGASGA
                     SGEKVKIGLSIAGLTMPYYVRMYDGFMAAAEKEGYEVVFADGGADAESTVKAVEDLIS
                     SDIDALAVSTNYSEALVDSFAKCKELGIPIFYIGNLNLIPEAKEFMTFWEGTRSDSAG
                     YLGGEWVAQYLDGLNYKKELNIVQVSLVADALQQRYDGFVKGLEDNGYTVNTINTVGD
                     LTREKSLSGCEDMLTAHDDIDIFFGTAGTSALGAYDATQNAGRTEVMVVGFDGEDEEL
                     QHIDEGGNYIATVCQQPALESEVTVEAIKTVLGGGTVEGGHETPAIVYGTGVGY"
BASE COUNT          483 a          632 c          636 g          407 t
ORIGIN      
        1 gaccgacttg tagccagcac cgaggaaaag gattaaaacg cagaaatgcc cgttatcggc
       61 catacagcca ataacgggca tttgtcattc atatacaagc tcggtcagaa cgatttcctc
      121 ggcacggctg cggatgttgt tcatccggcg tacccattcc atctggtcgg cggctttgag
      181 cgcttcggtc acaccctctt gctttgccat agcagaaacg atgatttcca tacgctcatt
      241 gcaggcttgg tcgatctcgg caagatgctg gtgcagtgtt ccgctcgtaa gatagtttgc
      301 atagagaaca ggtcgatgag ctttcagata gctacggcgc atacgcccat acttgccgat
      361 ctggtagtcc ggctcatctg gcaaatagag gtttgggata aggtaatcgc cctcttgccg
      421 gtatgtgccg cccatttgct caaacagtga tttcataaaa attagctcct ttgcgtgttg
      481 atttccagcg gttataaggt gtttttactc ctttcctaca actgctgttc cattcaccac
      541 aaatgagccg cagtcatatg tattaggtgg cagaaaccac cctttacata ttaactcttg
      601 cactcaaatg cgtgactgat caccacgctt tgatacagcc ggtcccggcc gggcgcaaac
      661 ccgccggggc gggcaataaa agacgaggag gtgaagcatg aaacgcgata gactactgaa
      721 ccgggagatc atctcggagg tcgccgcgct gggtcacacc gagtacctgt gcatcgcgga
      781 ctgcggcctg ccggtcccca agggggtcaa ggtgatcgac gtctcgatca ccgcaggcaa
      841 gccgtcgttt ttggatgtgt tggacgcggt ggacgaggag ctggtggtgg aatccatcat
      901 cctcgccgag gagatcgacg acaaaaacca gcccctggcc ggcgagatga acaagcggtt
      961 cgagggcaag cccatctgca aggtgcctca cgaggaattt aaaaagctga ccgaaaaggc
     1021 aaagtgcatc atccgcacgg gcgagaacag ctcgtatgcg aacgtcatcc tcgtgggcgg
     1081 ggtaaacttt taaatacggc gtggagggcc gcggcagaat gccgtaaggc cggccccgcc
     1141 tcaataggga aaatcaagga ggaaaattat gaaaaagttc ttgagcctcg tgctcgcagc
     1201 agcaatggtc tttgcgatga ccgcctgcgg cggcaacgac accagcagca ccccggcagc
     1261 gggcggcagc agcgccgcgg cgtccggtgc gtccggcgcc tcgggtgaaa aggtcaaaat
     1321 cggcctgtcc atcgccggcc tgaccatgcc gtactatgtg cgcatgtatg acggcttcat
     1381 ggcggccgcc gagaaagagg gctacgaggt cgtcttcgcc gacggcggcg ccgacgccga
     1441 gtccaccgtc aaggcggtcg aggacctgat cagcagcgac atcgacgccc tggccgtcag
     1501 caccaactac agcgaggcgc tggtcgacag ctttgccaag tgcaaagagc tgggcatccc
     1561 catcttctac atcggcaacc tgaacctgat ccccgaagcc aaagagttca tgactttctg
     1621 ggagggcacc cgttccgaca gcgccggtta cctgggcggc gagtgggtcg cgcagtacct
     1681 ggacggcctg aactacaaaa aagagctgaa catcgtgcag gtctcgctgg tcgccgacgc
     1741 gctgcagcag cgctatgacg gcttcgtcaa gggccttgag gacaacggct acaccgtcaa
     1801 caccatcaac accgtgggcg acctgacccg tgaaaaatcc ctgtccggct gcgaggacat
     1861 gctgaccgcg cacgacgaca tcgacatctt cttcggcacc gccggcacct ccgccctggg
     1921 cgcctatgac gccacccaga acgcgggccg caccgaggtc atggtcgtcg gcttcgacgg
     1981 cgaggacgag gagctgcagc acatcgatga gggcggcaac tacatcgcca ccgtctgcca
     2041 gcagcccgcc ctggagtctg aggtcacggt tgaggccatc aagaccgtgc tgggcggcgg
     2101 caccgtcgag ggcggccacg agaccccggc catcgtgtac ggcaccggcg tgggctat
//
LOCUS       DBMDYA010000518         2158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10617_length_2158_cov_1.425863, whole genome shotgun sequence.
ACCESSION   DBMDYA010000518 DBMDYA010000000
VERSION     DBMDYA010000518.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10617_length_2158_cov_1.425863"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="ACZPFK_11350"
     CDS             complement(<1..880)
                     /locus_tag="ACZPFK_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIR1406950.1"
                     /translation="MDETKARVPGRTRYSFGFGCIGRDAAYTLVTNFIMTYLTLAVGL
                     SNWQLAWVGVIMVAARVWDAVNDPMMGTIIDNTHTRWGKFKPYIITGAVLNSIFIVLL
                     FSDFAGLGKTAFLVVFAVTYILWGMTYTMNDISYWSMLPSLTVNPKEREKVSSLARIG
                     ANIGLFVVTAAVPVVTQMGKMTEMYRIIAVAIAALFIGCQLLVVLGVKEQKNAVTAAQ
                     SHTTLRGMVRIILKNDQLLAIIVCMLLFNIGYFTTTGFGMQFFYFDYGVYGGMEFTIF
                     AISIGVAQIVTLALYPK"
     gene            complement(1032..>2158)
                     /locus_tag="ACZPFK_11355"
     CDS             complement(1032..>2158)
                     /locus_tag="ACZPFK_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1406951.1"
                     /translation="GVGKTTVARIIAENSGMRLHKLNGTSASTADIRTVIGEIGTLMG
                     HGGILLYLDEIQYLNKKQQQSLLECIEDGSITLVASTTENPYFYVYTALLSRCTVFEF
                     KHLSPQDIKEALARAKAILEQEEDGRIEIEPAALDLMATACGGDVRKSVSSLELLAGA
                     AQPQDGVRRITLEMARQVAQRSSMNYDRDADNHYDTISALQKSIRGSDPDAAIHYLAR
                     LMEAGDLLSACRRLLVIAAEDVGLAYPQAIPIVKACVDSALQLGLPEAQLPLADAVVL
                     LATAPKSNSAHNAILAAREDLRRGRSGQVPRQLQNKHYDGADAKVKGQRYLYPHDYPD
                     HWVAQQYLPDELVGTVYYEFGPNKTEQAAKAYWDRVKGKK"
BASE COUNT          379 a          689 c          646 g          444 t
ORIGIN      
        1 ttttcgggta aagggccagc gtcacgatct gcgccacgcc gatggagatg gcgaagatcg
       61 tgaactccat gccgccgtac acgccgtagt cgaaataaaa gaactgcatg ccgaagcctg
      121 tggtggtgaa atagccgatg ttgaacagca gcatacagac aatgatggcc agcagttggt
      181 cgtttttgag gatgatcctc accatgccgc gcaaggtggt gtggctctgg gccgccgtga
      241 ccgcattttt ttgttccttg acgcccagca ccaccagcag ctgacagccg atgaacagcg
      301 cggcgatggc gacggcgatg atgcggtaca tttcggtcat cttgcccatc tgggtcacca
      361 ccggcaccgc cgccgtgacc acaaacaggc cgatattcgc cccgatgcgg gccagggagg
      421 agaccttttc ccgctctttg gggttgaccg tcaggctggg cagcatgctc cagtaagaga
      481 tatcgttcat cgtgtaggtc atgccccaga ggatgtaggt cacggcaaac accaccaaaa
      541 acgccgtctt gcccaagccc gcaaaatcag agaacagcag cacgatgaaa atgctgttga
      601 gcaccgcgcc ggtgatgatg tagggcttga atttgcccca gcgggtgtgg gtgttgtcga
      661 tgatggtgcc catcatgggg tcgttgaccg catcccagac acgggccgcc accatgatga
      721 cccccaccca ggccagctgc cagttgctca gccccacggc cagggtcagg taggtcatga
      781 taaaattggt caccagcgtg taggccgcgt ctcggccgat acagccgaag ccgaagctgt
      841 accttgtccg tcccggcacc cttgcctttg tctcgtccat cccttgtgcc ccctgtcttt
      901 ccaataaatt cccgcgcgtt ttcacgatat ccttttctgc ccgggttcaa agcttcaaaa
      961 aggccggagg gttgtcgctt tacaaccgct ccggccctcc cgtctttttt cgtcctgccc
     1021 gcgccgcccg gttacttttt ccccttgacc ctgtcccaat aagccttggc ggcctgctcg
     1081 gtcttgttgg gtccgaactc atagtagacc gtgcccacca gctcgtcggg cagatactgc
     1141 tgggcgaccc agtggtcggg gtagtcgtgg ggatagagat agcgctgccc ctttaccttt
     1201 gcgtccgcgc cgtcgtagtg cttgttctgc aactgccgcg gcacctggcc gctgcggccg
     1261 cggcgcaaat cctctcgcgc ggcgaggatg gcgttatgcg ccgaattgct cttgggcgcg
     1321 gtggccagca gcaccacggc gtccgccagc ggcagctgcg cctccggcag gcccagctgc
     1381 agggcgctgt ccacacaggc cttgacgatg gggatggcct ggggataagc gagccccacg
     1441 tcctcggcgg cgatgaccag cagcctgcgg caggccgaga gcaggtcccc cgcctccatc
     1501 aggcgcgcca gataatggat cgccgcgtcc gggtccgagc cgcggatgct cttttgcagc
     1561 gccgagatgg tgtcgtaatg gttgtcggca tcccggtcat agttcatgct gctgcgctgg
     1621 gccacctgcc gcgccatctc cagcgtgata cggcgcacac catcctgcgg ctgtgccgcc
     1681 ccggccagca gctccaggct gctcacgctt ttgcgcacgt cgccgccgca ggccgtggcc
     1741 atcaggtcca gcgcggcggg ttcgatctcg atcctgccgt cctcctcctg ctccaaaatg
     1801 gctttggccc gcgccagcgc ctccttgata tcctgcggtg agaggtgctt gaactcaaac
     1861 accgtgcagc gggagagcag ggcagtgtac acataaaaat aggggttctc ggtggtggag
     1921 gcgaccagcg tgatactgcc gtcctcgata cactccagca ggctctgctg ctgctttttg
     1981 ttcagatact ggatctcgtc cagatacagc aaaatgccgc cgtggcccat cagggtgccg
     2041 atctcgccga tcacagtgcg gatgtccgcc gtggaggcgg aggtgccgtt gagcttgtgc
     2101 agccgcatgc cgctgttttc cgcgatgatg cgcgccaccg tggtcttgcc cacccccg
//
LOCUS       DBMDYA010000519         2156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10625_length_2156_cov_1.962044, whole genome shotgun sequence.
ACCESSION   DBMDYA010000519 DBMDYA010000000
VERSION     DBMDYA010000519.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10625_length_2156_cov_1.962044"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="ACZPFK_11360"
     CDS             <1..798
                     /locus_tag="ACZPFK_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1406952.1"
                     /translation="LGMKSAGTAWATVLSQIAAFAASLVFMYRKRAHFQFDFKLRSFA
                     MKKAHLKVLIELGVPLAASSTFIHLSQLYCNAQVNAFGLVASAVNSVGNKVVRFANII
                     TSSINTGAAAMIGQNLGARKFDRVKKVTYIALALAFVMALLNCAVCVFAPTVVFRAFS
                     SDPAVIEAGVPFMHISVITFLLSAWQGPYNGVVTGSGHAKLSFVIGMLDGVVLRIGIS
                     LFLANVMGMGVYGYYYGNALARLAPCVICTWYFYSGRWAKRKLLSEA"
     gene            complement(1059..>2156)
                     /gene="larC"
                     /locus_tag="ACZPFK_11365"
     CDS             complement(1059..>2156)
                     /gene="larC"
                     /locus_tag="ACZPFK_11365"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR1406953.1"
                     /translation="NRDHEHGHDHGHDHDHEHNRDHEHGHDHEHGHEHGHDHDHGHDH
                     GHSHHHTGMEEIGGLLARLAVSEKVRRDAQAVYGLIAEAESHAHGVPVSQIHFHEVGD
                     MDAVADIVGVCMLMEELAPDRVLASPVHVGCGEVRCAHGVLPVPAPATARILRGVPVY
                     GGAVRGELCTPTGAALLKHFVSAFGGLPPLCVSAVGYGMGQKEFEAANCLRALLGETA
                     DSGEEIVELRCNLDDMTPEALGFAQEQLFAGGALDVYTCAVGMKKNRPGVLLSCMCRE
                     KQREALLRLLFRHTTTLGVREYRCARYALRRSERILETPYGPVRVKEAQGWGVHRSKP
                     EHDDLARIARQRGCSLAQAAELARAAKETAP"
BASE COUNT          319 a          769 c          676 g          392 t
ORIGIN      
        1 ctgggcatga aatccgccgg caccgcctgg gcgaccgtgc tctcccagat tgcggctttt
       61 gcggcctcgc tggtcttcat gtaccgcaag cgggcgcact tccagttcga ctttaagctg
      121 cgcagcttcg ccatgaaaaa ggcccacctg aaagtgctga tcgagctggg ggttccgctt
      181 gcggcctcga gcacgttcat ccacctctcg cagctgtact gcaacgcgca ggtgaacgct
      241 ttcgggctgg tggcttcggc cgtcaacagc gtgggcaaca aggtggtgcg ctttgccaac
      301 atcatcacct ccagcatcaa caccggcgcg gccgccatga tcggccagaa cctgggggcg
      361 cgcaaattcg accgggtgaa aaaggtcacc tacatcgcgc tggccctcgc ttttgtgatg
      421 gcgctgctca actgcgcggt ctgtgtcttt gcgcccacgg tggtctttcg cgctttcagc
      481 tccgacccgg cggtcatcga ggcgggggta ccctttatgc acatctcggt catcaccttt
      541 ctgctgtccg cctggcaggg cccctacaac ggggtggtta ccggctccgg ccacgccaag
      601 ctgagctttg tcatcggcat gctggacggc gtggtgctgc gcatcggtat cagcctgttt
      661 ttggccaatg tcatgggcat gggggtctac ggctattact acggcaacgc cctggcccgg
      721 ctggccccct gcgtcatctg cacctggtat ttctacagcg ggcgctgggc caagcgcaag
      781 ctgctgtcgg aggcctgacc ccaccggctc atactttgga ggagggaccg cggcacccag
      841 cccagtgggg cggccagaac gccgccgggg cgcgtccccc cctcctctgc cagacgatat
      901 caaacgacaa aaaagccgcc cggatggtgt atcgacaccg tccgggcggc ttttttcgct
      961 ctcagctcca ccttttgcgg cgggctcggt acttttcact tttgacgggc ttacagaacg
     1021 ggcgcccccc tgcctcgccg cggcggcaaa cgaagccgct agggcgccgt ctcctttgcc
     1081 gcgcgggcca gctctgccgc ctgggccagc gagcagcccc tctgccgggc gatgcgcgcc
     1141 agatcgtcgt gctccggctt ggagcggtgt acgccccagc cctgcgcctc cttgacccgc
     1201 accgggccat agggggtttc caggattcgc tcgctgcgcc gcagcgcata gcgcgcgcag
     1261 cggtactccc gcacccccag cgtggtggtg tgccggaaca gcagccgcag cagcgcctcg
     1321 cgctgctttt cccggcacat gcaggagagc agcacgccgg ggcggttctt cttcatgccc
     1381 acggcgcagg tgtatacgtc cagcgcgccg cccgcgaaca gctgctcctg ggcaaagccc
     1441 agcgcctccg gggtcatatc gtccaggttg cagcgcagct ccacgatctc ctcgccgctg
     1501 tccgccgttt cgcccagcag ggcgcgcagg caattggccg cctcaaactc cttttgcccc
     1561 atgccgtagc ccaccgccga gacgcagagc ggcggcaggc cgccgaacgc ggagacgaaa
     1621 tgcttcagca gcgcggcgcc ggtgggggtg cacagctcac cccgcaccgc gcccccatac
     1681 accggcacgc cccgcaggat gcgggccgtg gccggggcgg gcaccggcag cacgccgtgg
     1741 gcgcagcgca cctcgccgca gcccacatgc accggcgagg ccagcacccg gtcgggggcc
     1801 agctcctcca tcagcatgca gacccccacg atgtccgcca cggcgtccat atcgcccacc
     1861 tcgtgaaaat ggatctggga caccggcacc ccgtgggcat ggctctccgc ctccgcgatc
     1921 aggccataga ccgcctgcgc gtcccgccgc accttttcag agaccgccag ccgcgccagc
     1981 agcccgccga tctcctccat accggtgtgg tgatggctgt ggccgtggtc gtgcccatga
     2041 tcgtggtcgt ggccgtgctc gtgaccgtgc tcgtggtcgt gaccgtgctc gtggtcgcga
     2101 ttgtgctcat gatcgtggtc gtggccgtgg tcgtgaccgt gctcgtggtc gcgatt
//
LOCUS       DBMDYA010000520         2156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10627_length_2156_cov_1.675426, whole genome shotgun sequence.
ACCESSION   DBMDYA010000520 DBMDYA010000000
VERSION     DBMDYA010000520.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_2156_cov_1.675426"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..714)
                     /locus_tag="ACZPFK_11370"
     CDS             complement(73..714)
                     /locus_tag="ACZPFK_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HIR1406954.1"
                     /translation="MKEALDAVTRQRMERTAEALRRNNMEAYLTDGRAGVAPLVKSLL
                     HQGDTVSVGGSVSLDECGILELLRGGEYRFLDRYAPGLTREQQEELFRQSFFADAYLC
                     STNAVTENGELYNVDGNANRVAAMTFGPKSVILVVGTNKIVRDLDEAARRVKEVAAPA
                     NCKRLGCATPCAVTGQCADCKGDGRICCTTVIHQKQRAKGRIKVILVAEPLGY"
     gene            999..1814
                     /locus_tag="ACZPFK_11375"
     CDS             999..1814
                     /locus_tag="ACZPFK_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1406955.1"
                     /translation="MQKQKVTACIVTYGGFEEAAQGAESLLRCTQGVDLTLYLVDNAS
                     PDGTGEKLAQRFAGAQGVEVIELAENKGFGGGHNAVLERLDSVYHAVVNPDITLDSDA
                     VSALCGYLDEHPDVVMATPRLRFPDGREQQVAKRRPTLMALLARQLPLRFLKKYEDHY
                     LMLDEDLSTEKDVQFCSGCFFVIRTEAFQKLGGFDRRYFMYVEDADLTQEALRLGRVV
                     YWPGAQVTHAWHRNPSRQLSSFFQQLRSMGLYFKKWGFRWGFSLREPPAKEKT"
     gene            1836..>2156
                     /locus_tag="ACZPFK_11380"
     CDS             1836..>2156
                     /locus_tag="ACZPFK_11380"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
                     /protein_id="HIR1406956.1"
                     /translation="MKGIVLAGGAGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLA
                     GIREILIISTPEDTPRFEHLLGDGGDFGLSLSYKVQPSPDGLAQAFLLGEEFIGGEPC
                     AMVLG"
BASE COUNT          362 a          705 c          699 g          390 t
ORIGIN      
        1 ctcacgccgt ccgccttttc atgaggcttt tcgccgcatt tcggcgcgcg gcgcggctga
       61 cggagcgcgc ggtcaatagc ccagcggctc ggcgaccaaa atcaccttga tgcgcccttt
      121 ggcccgctgc ttttgatgga tcaccgtggt gcagcagatg cggccgtcgc ccttgcagtc
      181 ggcgcactgg ccggtgacgg cgcagggggt ggcgcagccc agccgcttgc agtttgccgg
      241 ggcggccacc tccttgaccc ggcgcgcggc ctcgtccagg tcgcgcacga tcttgttggt
      301 tcccaccacc aggatgacgc tcttggggcc aaaggtcatg gcggccaccc ggttggcgtt
      361 gccgtccaca ttgtacagct cgccgttctc ggtcacggcg ttggtgctgc acagataagc
      421 gtccgcgaaa aagctttggc ggaacagctc ctcctgctgc tcccgggtca gccccggcgc
      481 atagcgatcg agaaagcggt actcgccgcc gcgcagcagc tccagaatgc cgcactcgtc
      541 cagcgagacg ctgccgccca ccgagacggt gtccccctgg tgcagcaggc tcttcaccag
      601 cggcgccacc ccggcgcggc cgtcggtgag ataggcctcc atgttgttgc ggcgcagcgc
      661 ctcggcggtg cgctccatcc gctgccgggt cacggcgtcc agagcttctt tcatatcgtc
      721 ttcctccctt ttttgaaagg ccggcctcgg gcgccggcgg cggtgcttat gataagacca
      781 tcataatacg cgggctgcaa aaagacaagc gggcggggtg cccccctccc ctccgcgtca
      841 gccggtcttc tccccgccgg gcggcccgcg ccccgtcctt tgacaaaaag cgttgattag
      901 ccgcggggcg gatggtataa taatcaagat aaaggcctgt ccccggacaa gcccaaagag
      961 aactgggcgc gggcgctgtc ccgcgcggat ggagacaaat gcaaaaacag aaagtcaccg
     1021 cctgcatcgt cacctacggc ggttttgaag aagcggcgca gggggccgag tccctgctgc
     1081 gctgcaccca gggggtcgac ctgacccttt atctggtgga taatgcctcc cccgacggca
     1141 ccggcgaaaa gctggcgcag cgctttgccg gcgcgcaggg cgtcgaggtg atcgagctgg
     1201 cggagaacaa gggcttcggc ggcggccaca acgcggtgct ggagcggctg gacagcgtct
     1261 accacgcggt ggtcaacccg gacatcacgc tggacagcga cgccgtcagc gccctgtgcg
     1321 gttatctgga cgagcacccg gacgtggtga tggccacgcc gcggctgcgc ttcccggacg
     1381 ggcgggagca gcaggtggcg aagcgccgcc ccaccctgat ggcgctgctg gcgcggcagc
     1441 tgccgctgcg ctttctcaaa aagtatgagg atcactacct gatgctggac gaggacctct
     1501 ccacggaaaa ggacgtgcag ttctgttccg gctgcttttt cgtcatccgc accgaggctt
     1561 ttcaaaaact gggcgggttt gaccgccgct acttcatgta tgtggaggac gccgacctca
     1621 cccaggaggc gctgcggctg gggcgggtgg tttattggcc gggggcgcag gtcacccatg
     1681 cctggcaccg caacccctcg cggcagctgt ccagcttttt tcagcagctt cggtccatgg
     1741 gactctattt caaaaaatgg ggctttcgct ggggcttttc cctgcgggag ccgcctgcga
     1801 aagaaaaaac ataacaaggc aaaggagcgt tcagtatgaa agggatcgta ctcgcaggcg
     1861 gggcgggcac ccgcctgtac ccgctgacca tggtgaccag caagcagctg ctgccggtct
     1921 acgacaagcc gatgatctat tacccgctct ccaccctgat gctggcgggc atccgggaga
     1981 tcctcatcat ctccacgccg gaggacaccc cccgctttga gcatctgctg ggggacggcg
     2041 gcgatttcgg cctctccctc tcctacaagg tgcagccctc gccggacggc ctggcgcagg
     2101 cgtttctgct gggggaggag ttcatcggcg gcgagccctg cgccatggtg ctgggg
//
LOCUS       DBMDYA010000521         2145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10696_length_2145_cov_1.914384, whole genome shotgun sequence.
ACCESSION   DBMDYA010000521 DBMDYA010000000
VERSION     DBMDYA010000521.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_2145_cov_1.914384"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            47..574
                     /locus_tag="ACZPFK_11385"
     CDS             47..574
                     /locus_tag="ACZPFK_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016121.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1406957.1"
                     /translation="MKSKSNTFENIVKPILVLTVICIVVSGLLALTNMVTAPVIARNA
                     QRKADAGRIALLPAADAFQQIEYSDDVVSEVWAAENGAGYVITGSAKGYGGDLPVMVG
                     IGADGVITAIQVMDNSETPNVGKKTMEPAFTDQFKGKSGSDEVAAISGATISSTAVFK
                     VVDAAMAAYQTVKGA"
     gene            578..1273
                     /gene="rsxE"
                     /locus_tag="ACZPFK_11390"
     CDS             578..1273
                     /gene="rsxE"
                     /locus_tag="ACZPFK_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR1406958.1"
                     /translation="MEKEKKTGKLSILTNGLLKENPSLRLVLGTCPTLAITTAVFSSI
                     GMGLAATFVLIFSNMAISACRKIIPDKVRIPAFITIIAGFVTVVMMLVQAFVPDLYDA
                     LGVYLPLIVVNCIILGRAEMFASKNKVLDSALDGLGMGLGFTLTLTLMGFVRELLGAG
                     TLFGLQILPQGVEPVAILASPPGGFFVFGVLMALAILLDQKRGKKVDADPEKMGCAGC
                     PNSKKCNGEGGCE"
     gene            1273..1869
                     /locus_tag="ACZPFK_11395"
     CDS             1273..1869
                     /locus_tag="ACZPFK_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1406959.1"
                     /translation="MAAKLAFIFFSMILTNNYVLVKFLGICPFLGVSKKLDSAVGMSA
                     AVTFVMVVATLATWPVYTYLLEPNGLAYLQTIVFILVIAILVQLLETVMKKFMPPLYK
                     ALGIYLPLITTNCTILGVTILNLDSGYSYLESIVCALGAGLGFMLAMVLFSGVRKRLE
                     DAQPPKCFEGLPITLVAASIVSLSFTGFGGLIENIFGM"
     gene            1885..>2145
                     /locus_tag="ACZPFK_11400"
     CDS             1885..>2145
                     /locus_tag="ACZPFK_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1406960.1"
                     /translation="MSIVLAIAIVSVIGLVGAAILVVAAKYMTVEEDPRVGQVTEALP
                     GANCGACGYAGCADYAKAIVSGEAQVGRCVPGGQKSADAVAAI"
BASE COUNT          390 a          671 c          674 g          410 t
ORIGIN      
        1 tcaacgacct cacccgccag aaaccgctgg gaggtgcaaa aaagaaatga aaagcaaaag
       61 caacacgttt gaaaatatcg taaagcccat tttggtgctg accgtcatct gtatcgtggt
      121 gtccggcctg ctggccctca ccaacatggt gaccgcgccg gtcatcgccc gcaacgcgca
      181 gcgcaaggcg gatgcgggcc gcatcgccct gctgccggcg gccgacgcct ttcagcagat
      241 cgagtacagc gacgacgtgg tcagcgaggt ctgggccgcc gagaacggcg cgggctatgt
      301 gatcaccggc tcggccaagg gctacggcgg cgatctgccg gtcatggtgg gcatcggcgc
      361 cgacggcgtc atcaccgcca tccaggtcat ggacaactcc gagaccccca acgtgggcaa
      421 aaagacgatg gagcccgcct ttaccgacca gttcaagggc aaaagcggct ccgacgaggt
      481 ggccgcgatc tccggcgcca ccatctcctc caccgccgtc ttcaaggtgg tggacgccgc
      541 catggcggcc tatcaaacgg taaagggggc ctaagagatg gaaaaagaga aaaagaccgg
      601 caagctgtcc atcctcacca acggcctgct caaagagaac cccagcctgc ggctggtgct
      661 gggcacctgc cctacgctgg ccatcaccac ggcggtgttc agcagcatcg gcatgggcct
      721 ggccgccact tttgtgctga ttttctcgaa tatggcgatc tccgcctgcc gcaagatcat
      781 ccccgacaag gtgcgcatcc cggcgttcat caccatcatc gccggctttg tgaccgtggt
      841 catgatgctg gtgcaggcct ttgtgcccga cctgtacgac gcgctgggcg tctatctgcc
      901 cctgatcgtg gtcaactgca tcatcctcgg ccgcgccgag atgttcgcca gcaagaacaa
      961 ggtgctggac tcggcgctgg acggcctggg catggggctg ggctttacgc tgaccctgac
     1021 cctgatgggc tttgtgcgcg agctgctggg cgctggcaca ctgttcggcc tgcagatcct
     1081 gccccagggc gtcgagccgg tggcgatcct tgccagcccc ccgggcggct tcttcgtgtt
     1141 cggcgttctg atggccctgg ccattttgct ggaccagaag cggggcaaaa aagtggacgc
     1201 ggacccggag aagatgggct gcgccggctg cccgaacagc aagaaatgca atggggaagg
     1261 agggtgtgaa taatggccgc gaagcttgca ttcatctttt tcagtatgat cctgaccaac
     1321 aactatgtgc tggtcaaatt tctcggcatc tgccctttcc tcggcgtgtc caaaaagctg
     1381 gactccgcgg tgggcatgtc cgccgccgtc acctttgtga tggtggtggc gaccctggcc
     1441 acctggcccg tttacaccta tctgctggag ccgaacggcc ttgcctatct gcagaccatc
     1501 gtcttcatcc tggtcatcgc cattctggtg cagctgctgg agacggtgat gaaaaagttc
     1561 atgccgccgc tgtacaaggc cctgggcatc tatctgcccc tgatcaccac caactgcacc
     1621 atcctgggcg tcaccatcct caacctggac agcggctaca gctatctgga gagcatcgtc
     1681 tgcgccctgg gcgcgggcct tggctttatg ctggcgatgg tgctgttctc cggcgtgcgc
     1741 aagcggctgg aggacgccca gccccccaaa tgctttgagg ggctgcccat cacgctggtc
     1801 gcggccagca tcgtgtcgct ctcctttacc gggttcggcg gcctgatcga aaacatcttc
     1861 ggcatgtgag ggagggctga gagcatgagt attgtattag cgatcgcgat tgtttcggtg
     1921 atcggcctcg tgggcgcggc catcctggtg gtcgccgcca agtatatgac ggtggaggag
     1981 gacccccgcg tgggccaggt caccgaggcc ctgccggggg ccaactgcgg cgcctgcggc
     2041 tatgcgggct gcgcggacta cgccaaggcc atcgtgtccg gcgaggcgca ggtgggccgc
     2101 tgtgtgcccg gcgggcagaa gtcggccgac gccgtggccg ccatc
//
LOCUS       DBMDYA010000522         2145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10699_length_2145_cov_1.649706, whole genome shotgun sequence.
ACCESSION   DBMDYA010000522 DBMDYA010000000
VERSION     DBMDYA010000522.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10699_length_2145_cov_1.649706"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="ACZPFK_11405"
     CDS             complement(<1..739)
                     /locus_tag="ACZPFK_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1406961.1"
                     /translation="MERIKAIVFNKHGVGHMTRVARFPRPGETVRALEWHFGEDAGKG
                     TNVAVALGRLGVKNAFVCKVGADEGGELGAKWLKSAGVDLKHYMMCRQVKTDVGLVIT
                     QSDGENMIIGSAQHPCYMSREELFAAIEDYREAEFFLTGLEIDPKLPIEGCRYAKARG
                     MTTLLNASPLVEEIRQPLDCVDYLFVNELEGTQLSGQEKGGHSWYQIAELVRRRYQPQ
                     TVVMTLGNKGCVLCNAEGSKFFTSYEAP"
     gene            complement(757..1356)
                     /locus_tag="ACZPFK_11410"
     CDS             complement(757..1356)
                     /locus_tag="ACZPFK_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase family"
                     /protein_id="HIR1406962.1"
                     /translation="MDEYLDWESRLIPEDAPYGSLHAVMKKNVFSKIDPALMCPAEQI
                     IFPDPCNLDYYDDKIEELGGIDTIWAGIGCKGLIAFCEAPHSRYYRISQEQYKNSKTR
                     IVELNEDTLVALSQRRFGGYYDKIPPKAVTVGMKSILSAKRIVCMLRCGAWKQTALRV
                     MLLCKQTTLEYPVTFAMKYIPEKIVFCDEETLEHPLCKR"
     gene            complement(1774..>2145)
                     /locus_tag="ACZPFK_11415"
     CDS             complement(1774..>2145)
                     /locus_tag="ACZPFK_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406963.1"
                     /translation="RSPLYEKGVTPAIDAAIKPLGSFNGFASTDYGDVMHICPGVQNT
                     DCTAATLAGGHSWMVTACSGSSIGMKGMLRAAKVMAAGAWQMVERPELLKAAKEEFEA
                     AMGGKKYVCPITDEVAKPFEK"
BASE COUNT          454 a          656 c          536 g          499 t
ORIGIN      
        1 agggcgcctc ataggaagta aaaaatttgc tcccctccgc attgcaaagg acgcagccct
       61 tgttgcccag cgtcatcacc actgtttgag gctggtaacg cctgcggacg agttcggcga
      121 tttgatacca gctatgcccc cctttctcct ggccgctcag ctgggtcccc tccagctcgt
      181 tgacgaagag gtaatccaca caatccagcg gctgccggat ctcttccacc aaggggctgg
      241 cgttgagcag agtcgtcatc ccccgcgcct ttgcatagcg gcatccctcg atcggcaact
      301 tgggatcgat ctccaggccg gtcagaaaga actccgcctc ccggtaatcc tcgatagcgg
      361 caaacaattc ttcccggctc atataacagg ggtgctgtgc gctcccgata atcatgttct
      421 cgccgtcgct ctgggtaatc accagcccca cgtccgtttt gacctggcga cacatcatgt
      481 aatgcttcaa atccaccccg gcacttttca accatttcgc accgagctca ccgccctcat
      541 ctgcccccac tttgcataca aaagcatttt ttacgcccaa gcgccccaac gccaccgcaa
      601 cattcgtccc ctttccggcg tcctctccaa aatgccactc caaagcccgc acggtttcac
      661 cgggccgtgg aaaccgcgcc acgcgcgtca tatgtccaac tccgtgtttg ttaaaaacaa
      721 ttgcttttat tctctccatt gcaaacttcc cacagcttac cttttacaga gcggatgttc
      781 cagtgtttcc tcatcgcaga acacgatttt ttcagggata tatttcattg caaacgtgac
      841 ggggtattcc agcgtcgtct gtttacacaa aagcatgacc ctcagcgccg tctgcttcca
      901 cgcgccgcaa cgcagcatgc aaacgatcct cttcgcgctc aggatcgatt tcatgcccac
      961 cgtaaccgct ttagggggaa tcttgtcata atacccgcca aagcgccgct gcgacagggc
     1021 aaccaaggta tcctcattga gctccacgat cctggtcttg gagtttttat actgctcctg
     1081 actgatgcgg tagtagcgtg aatgaggtgc ctcgcaaaat gcgatcaggc ccttgcagcc
     1141 aatgccggcc cagatggtgt cgatcccgcc cagctcctcg atcttgtcgt catagtagtc
     1201 gagattgcag ggatccggaa agatgatctg ctctgcaggg cacatcagcg caggatcaat
     1261 tttgctgaag acgttttttt tcatcacagc gtgcaggctg ccataaggtg cgtcttccgg
     1321 gatcaaacgg ctttcccagt ccagatactc gtccatatgg tatccgcgta tcagcactct
     1381 gcgggcgggc gctcaagagt tcaaggctct ggaccgggac agccccgagg accgctttta
     1441 gggcttcgcg atgcaaaaca aataaaaagc agccgcagcg aagcggaggc tattttttcg
     1501 gccatgtgtc ccgtcttcgg gccacagtcc tggagataaa gcatttttat tgcatagcaa
     1561 caaaaaatag ctgctgtgcg gcttttttgg ctatccggcc gctcctgcaa ccgggaccgt
     1621 taagacaaac ttttttcggc ccgccgtttc ggttgccgcg gcagcggtga aaaataggac
     1681 caggaagatc gttgtttttt ataattacag caacaaaaag cgcaaacaaa aagagagatg
     1741 cacagcgcat ctctcttttt gttgtcggaa cgcttatttc tcgaaaggct tcgcaacctc
     1801 gtcggtgatc gggcaaacgt attttttgcc gcccatggct gcctcgaact cctctttggc
     1861 cgctttcagc agctcggggc gctcgaccat ttgccaggcg ccagccgcca tgaccttggc
     1921 cgcccgcagc atccctttca tgccgatgga cgagccggag caggcggtga ccatccagct
     1981 gtggccgccc gcaagggtgg ccgcggtgca gtcggtgttc tgcacgcccg ggcagatgtg
     2041 catcacatca ccgtagtcgg tggaggcaaa gccgttgaag ctgcccagcg gcttgatcgc
     2101 ggcgtcgatg gccggggtga cccctttttc atacagcggc gagcg
//
LOCUS       DBMDYA010000523         2141 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10726_length_2141_cov_1.243137, whole genome shotgun sequence.
ACCESSION   DBMDYA010000523 DBMDYA010000000
VERSION     DBMDYA010000523.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_2141_cov_1.243137"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(377..451)
                     /locus_tag="ACZPFK_11420"
     tRNA            complement(377..451)
                     /locus_tag="ACZPFK_11420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Gln,seq:ttg)
     gene            complement(518..594)
                     /locus_tag="ACZPFK_11425"
     tRNA            complement(518..594)
                     /locus_tag="ACZPFK_11425"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(558..560),aa:His,seq:gtg)
     gene            complement(601..677)
                     /locus_tag="ACZPFK_11430"
     tRNA            complement(601..677)
                     /locus_tag="ACZPFK_11430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Arg,seq:tct)
     gene            891..1169
                     /locus_tag="ACZPFK_11435"
     CDS             891..1169
                     /locus_tag="ACZPFK_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR1406964.1"
                     /translation="MDAFTISFLIVINLVTLLLCWSDKRAAVNHTRRIPERLLMILTI
                     AGGSAGMLAGMLIFRHKTKDSRFRITVPVVLVAETVFCILFYTMPHNT"
     gene            1267..>2141
                     /locus_tag="ACZPFK_11440"
     CDS             1267..>2141
                     /locus_tag="ACZPFK_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1406965.1"
                     /translation="MQGKKVTIEDVAARLGLAKSTVSKALSDATDINQETRERVLHCA
                     SAMGYHMKKRHAAPGGEVAVFVEGVEYENVDQFGHEILLGFQSEASKAHFGVRIVPLG
                     QGEDFRARYEELLAGPRFLGSFFIGFRPHSSFISEFEAAGRPAVLLDNDYDSALTAHV
                     GSDNDAGMEYALAHLAALGHRRVGFLGGEEESCVTAERLAAFEAAARRHGIEAEGRVA
                     FASFYRDYDPSLALRLVEGGATALVCCSDRIALAALKKLAAAGVAVPGQVSVVGYDGI
                     PLAEYASPALTTLSQ"
BASE COUNT          414 a          605 c          692 g          430 t
ORIGIN      
        1 cgtgctgctg atggccctga tgaccgaacg cgcgggcaag ggctctgcgg caaaggacgc
       61 ccacgcctga agcccctgcg gcgattgccc ctgacgcgcc cggaccgctt gacggcccga
      121 atacctttga tgtcaaaaag gggtgcaaaa ataaaaggcc cttgaggcct ttcggcgccc
      181 aggggctttg cagcgatgta ataaaatcgc tttttaaggg attcgcctcc ggcggcagac
      241 caagcggtat ctgccgcttt ccctttctgg ggtgacagaa gatttttaga actggggcgg
      301 cgaaagccga caccaagagg ggacagcgta cccttatgaa aaaaacgagt gaaagcaacc
      361 gctttcactc gttttttggt tgggctggct ggattcgaac cagcggatgc aggagtcaaa
      421 gtcctgtgcc ttaccgcttg gcgacagccc aagatagagg gatggtctgt aaagaaaaaa
      481 gcgaactcaa aggtttgaat tcgctttctt ttttaaatgg ggtgggagat gggactcgaa
      541 cccacgacac ccagatccac aatctggtgc tctaaccaac tgaactacac ccaccatatt
      601 tggtgcgccc gaagggactc gaacccctgg cccactgctt agaaggcagt tgctctatcc
      661 acctgagcta cgggcgcatg ccgtgcccta ccgagctgcg tattatatga tactataccg
      721 tccgggcaaa agtcaaggcc tttgcgaact ttttttgtcg gggagcaaaa aagcagaggc
      781 gcggcccgca gccggcgggt aattctgcaa aatcggttgg aaaaccggcg tttttctgct
      841 atactttgct ttattgagaa gaaaatctgg atgaataaag gggaagcgat atggacgctt
      901 ttacgatctc gtttttgatc gtcatcaacc tggtcaccct gctgctctgc tggagcgata
      961 agcgggcggc ggtgaaccac acgcgccgca tcccggagcg gctgctgatg atcctcacca
     1021 tcgccggcgg ctcggcgggc atgctggcgg gcatgctgat cttccgccac aagaccaagg
     1081 attcgcgttt tcgcatcacc gtgccggtgg tgctggtggc ggagacggtt ttttgcatcc
     1141 tgttttatac gatgccgcac aatacctgac gcggggcggg gccacagctg tggccccttt
     1201 ttcacatacc gggccgcttt gccgccgtgg cggcccgtga ggacggcagc tttggagggg
     1261 tgagggatgc aggggaaaaa agtgaccatc gaggatgtgg ccgcccgcct ggggctggcc
     1321 aagagcacgg tcagcaaggc gctctcggac gccaccgaca tcaaccagga aacgcgggag
     1381 cgggtgctgc actgcgcctc cgccatgggc tatcacatga aaaagcggca tgcggcgccc
     1441 ggcggcgagg tggcggtctt tgtcgagggc gtggagtacg aaaatgtgga ccagtttggg
     1501 catgagattt tgctgggctt tcagtcggag gcgagcaagg ctcatttcgg ggtgcgcatc
     1561 gtgcccctgg ggcaggggga ggattttcgc gcccgctatg aggagctctt ggcgggaccg
     1621 cgctttttgg gcagcttttt tatcggcttt cgcccccatt cctcctttat cagcgagttt
     1681 gaggcggcgg gccgccctgc ggtgctgctg gacaacgact atgattccgc cctcaccgcc
     1741 catgtgggca gcgacaacga cgcgggcatg gagtatgcgc tggcgcatct cgccgcgctg
     1801 gggcaccggc gcgtgggctt tctcggcggc gaggaggaga gctgtgtcac cgcagagcgg
     1861 ctggccgcct ttgaggccgc ggcccgccgc cacggcatag aggcggaggg ccgcgtcgcc
     1921 tttgccagct tttaccgcga ttacgacccc tcgctggcgc tgcgcctggt cgagggcggg
     1981 gccaccgcgc tggtgtgctg cagcgaccgc atcgccctgg cggcgctgaa aaagctggcg
     2041 gcggcggggg tagccgtgcc cgggcaggtc agcgtggtgg gctacgacgg catcccgttg
     2101 gcggaatacg cctcgcccgc cttgaccacc ctatcacaga a
//
LOCUS       DBMDYA010000524         2140 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10731_length_2140_cov_1.658656, whole genome shotgun sequence.
ACCESSION   DBMDYA010000524 DBMDYA010000000
VERSION     DBMDYA010000524.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10731_length_2140_cov_1.658656"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            216..809
                     /locus_tag="ACZPFK_11445"
     CDS             216..809
                     /locus_tag="ACZPFK_11445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1406966.1"
                     /translation="MTDQNTPRLETDRLILRKFAPKDLQALLRIYGDRQANTYLPWFP
                     LRTEREAQALLEQKYLAGYARPTGYLYAVCRKERDVPVGYVHLGVEEPYYLGYGLRRA
                     FWGRGLAAEAARAVIDRARADGVPYLTATHDVDNPRSGAVMKKLGMRYQYSYREQWQP
                     KDIPVVFRLYQLNLDGQERVYQGYWQASKQHFVEEDV"
     gene            986..1939
                     /locus_tag="ACZPFK_11450"
     CDS             986..1939
                     /locus_tag="ACZPFK_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR1406967.1"
                     /translation="MTSDKTVVLNAARVNYDGKAGFEGIASQVTVYDDTPPEKMLERV
                     QGHAVVVTKEIPVPGELIRQFPATVKLLCEAGTGYNNIDLAAAREKGITVCNVPAYSS
                     ERVAQTVLLLMLNLASSMTRQQRMLARGDHTNFTRCLGVDHVELNGKTLGVVGVGNIG
                     RAVIRAAQALEMNILATAHRPRPDEGGIHYVPLEELLRQSDFVSLHCPLTEETRHMIN
                     GRTLALMKPTAFVVNTARGALIDEAALIEALQAGRIAGAGLDVQESEPPAEDSPLYTL
                     ENVVLTPHMGWKGLETRQRLVAGVGKNIRAFCEGRPVNVIV"
BASE COUNT          439 a          616 c          751 g          334 t
ORIGIN      
        1 caggccccag atgcgtcagg acgtgtgctg agctgaactt gtgcttcctc cccgcagggc
       61 aaagcccttt gtggtcggag actcagcttc attatagcgg atgcggggca aagaatcaag
      121 aggggcgcgg gcgcttttgt ggcacggagg ctttacggcg caggaaagag cggttagaat
      181 agaaccgtaa agaaaagcga gggggagaaa aggcgatgac tgaccaaaac acgcctaggc
      241 tggagaccga ccggctgatc ctgagaaaat ttgccccaaa agacctgcag gcgctgctgc
      301 ggatttacgg cgaccggcag gccaacacct atctgccctg gttccccctg cgcaccgaga
      361 gggaggcgca ggccctgctg gagcaaaaat atctggcggg ctatgcgcgc cccacaggct
      421 atctctatgc cgtctgcaga aaagagcggg acgtccccgt cggctatgtc catctgggcg
      481 tggaggaacc ctactacctg ggctacggcc tgcggcgggc attttggggc agggggcttg
      541 cggcggaggc ggcgcgggcg gtgattgacc gggccagagc ggacggagtg ccctatctca
      601 cagccaccca tgacgtcgac aacccacgca gcggcgcggt gatgaaaaag ctgggcatgc
      661 gctatcagta cagctaccgg gagcagtggc agcccaagga tatcccggtc gttttcaggc
      721 tgtatcagct gaacctggat gggcaggagc gggtttacca ggggtattgg caggcctcaa
      781 agcagcattt tgtagaggaa gacgtgtgaa aggggggggc gcccctttcc aagcgtctgc
      841 ggcgcccgcg tcaaaagggg cagcccgcgc gccgggctgc ggaggccttt gccggggaca
      901 gggctgcgga gggctttatt gcaacggccg cccggctgtg ctacactcag attgaaaaat
      961 tttctctgcg aaaggggaac acgaaatgac gagcgataag acggtcgtgc tcaacgccgc
     1021 acgcgtgaat tatgacggca aagcgggctt tgaggggatc gcgtcccagg tgacggtcta
     1081 cgacgacact ccgccggaaa aaatgctgga acgggtgcag ggccacgcgg tggtggtcac
     1141 caaggagatc ccggtaccgg gggagctgat ccggcagttc cccgcgacgg tgaagctgct
     1201 gtgtgaggcg ggcaccggct acaacaacat cgaccttgcc gccgcgcggg agaaagggat
     1261 caccgtctgc aacgtgcccg cctacagcag cgagcgggtg gcccagaccg tgctgctgct
     1321 gatgctgaac ctggcctcgt cgatgacaag gcagcagcgg atgctggccc ggggcgacca
     1381 caccaacttc acccgctgcc tgggggtgga ccatgtggag ctgaacggca agacgctggg
     1441 cgttgtgggc gtgggcaata tcggccgggc ggtgatccgg gccgcgcagg cgctggagat
     1501 gaatatcctg gcgaccgccc accgcccccg cccggatgag ggcggcatcc actatgtgcc
     1561 gctggaagag ctgctgcggc agagcgactt cgtctcgctg cactgcccgc tgacggagga
     1621 gacaaggcac atgatcaacg ggcggacact ggccctgatg aaaccgaccg cttttgtcgt
     1681 caacacggct cgtggggcgc tcatcgacga ggcggcgctc atcgaggccc tgcaggccgg
     1741 ccgcatcgcg ggggcgggcc tggacgtgca ggagagcgag cccccggcgg aggacagccc
     1801 gctctacacg ctggaaaacg tggtgctcac cccgcacatg ggctggaaag gccttgagac
     1861 ccgccaaagg ctggtggcgg gggtgggcaa aaatatccgc gccttttgcg agggcaggcc
     1921 ggtcaacgtg atcgtttaaa gcaagcgcgc cgccggggcc gcgccgcttt ttgggggcgc
     1981 acccagagca ggccccgctt gcggcgatta aaaccggccg tttttcccat actgtcagca
     2041 gaccgcaggg acggtctgct gaagagaaag gagagacggc aatgagagcg aaagtgaacg
     2101 agaactgcat cggctgcggc ctgtgcgcgg gcacctgccc
//
LOCUS       DBMDYA010000525         2130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10788_length_2130_cov_1.472154, whole genome shotgun sequence.
ACCESSION   DBMDYA010000525 DBMDYA010000000
VERSION     DBMDYA010000525.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_2130_cov_1.472154"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="ACZPFK_11455"
     CDS             <1..195
                     /locus_tag="ACZPFK_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406968.1"
                     /translation="KLRNVPVECLPVFTDKSVQLAVPKSSRVFRQKDLMSTLEKEKYL
                     EDKGLDQELVAGLLREALQK"
     gene            complement(1028..>2130)
                     /locus_tag="ACZPFK_11460"
     CDS             complement(1028..>2130)
                     /locus_tag="ACZPFK_11460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1406969.1"
                     /translation="AKVTLVCGEGIIPYNRPGLSRLVAEGAGFESVALEPYGWYEARR
                     IELVCDARVERVDPDRREALLSDGRALPYTALLLATGANAFCPVKGGAGAPPVRVLRS
                     FADASALVEAVRPGARALVVGGGILGLEAAMALYRRGARVRVVELAERVLSIQADPFA
                     SGRIEQALRALGMEFDTGVSVSAVEADGAHLSDGRVLPAELVLVSAGIRSELDLARGL
                     GLALGRGICVDASMKTSLPGIYAAGDCAEFEGRVAGLWGAALEMGRTAGRAMCGCEAV
                     YRPPVPATAFEAPGISLFAVGSVNGEGLQAVERRDDEAGVYRKLALRDGRLVGVLMLG
                     RPAGGAAAVAAVERGAALDEALPLLQQQGEKV"
BASE COUNT          358 a          793 c          657 g          322 t
ORIGIN      
        1 aagctgcgca acgtgccggt ggaatgcctg ccggtcttca ccgacaaatc ggtgcagctg
       61 gcggtgccca agagcagccg cgtctttcgc cagaaagatc tgatgagcac cctggaaaaa
      121 gagaagtatc tcgaggacaa ggggctggat caggagctgg tcgcgggcct gctgcgcgaa
      181 gccctgcaaa agtgaaccgg ccgcagcggc cccgcgccgc ccgtgccttt tcatgatata
      241 ctaaaacccc aaaaaagaca aacgcccgcc ccgccgcacg caggggcggg cgtttgttgc
      301 cccagggccc cggccggaaa atcccccaaa gcttctcgcg agggccccgc tggggagccg
      361 ggccggcggc ccccaaagca gcccgcggct gtttttccat tgtcctctca ttctgctcca
      421 cgctcggaca ccgccccacg gcctttttca gagcggccga tcatcccgcc ccctgggctg
      481 tgctggtgtc ggttcagcgc ttttttcagg ccctcctgcg gaggcagccg tttggccccc
      541 tttcagggga gccgccgcgc cgggacgaaa gagggacggg acgcgcagaa aaaatcaggg
      601 tctgacctcc tcgctcccct tttttgctgt agcagagaga cgggcaaggg ctgcccatgt
      661 ttattccccg ccgccgctgc cgctggaacg gaggcgcgca acaagcgctt tccctctgcg
      721 gccccgggcg gcttcccttt ctattgtgcg cggggagggc acccggcatc cccgcgccgg
      781 ggcaaatact ttcgcatccg gcccaagaca aagagaaaac ccgcagcttg cgctgcgggt
      841 tttcttcaag gggtgtgttt tggtgtgagg aggaatcatg tacagaaagc cggttgcggc
      901 ttccgtcttt ctgtgattct cattataggg gccagttgag acgtttttat gaaacgcctg
      961 tgaataaaaa atgaatttgg agaaaagttt gtgacagacg ccaaaaagcc cggtgctgcg
     1021 ccgtttttca caccttttcg ccctgctgct gcagcagggg cagggcctcg tccaacgcgg
     1081 cgccccgctc caccgcggcc accgccgccg ccccgcccgc agggcggccc agcatcagca
     1141 cgcccaccag gcggccgtcc cgcaaagcga gcttgcggta gacgccggcc tcatcgtccc
     1201 gccgctccac cgcttgcagc ccctcgccgt tgacgctgcc caccgcgaac aggctgatgc
     1261 ccggcgcctc gaaagccgtc gcgggcaccg gcggccggta aaccgcctca cagccgcaca
     1321 tcgccctgcc cgccgtgcgc cccatctcca gcgccgcgcc ccacaggccg gccacccggc
     1381 cctcgaactc ggcgcagtcg cccgccgcgt agatgccggg caggctggtt ttcatcgagg
     1441 cgtccacgca gatgccccgc cccagcgcca ggcccagccc gcgcgccaga tccagctcgc
     1501 tgcggatgcc cgcgctcacc aaaaccagct cggcgggcaa gaccctgccg tccgacaggt
     1561 gggcgccgtc tgcctcgacc gcgctcaccg agacgccggt gtcaaactcc atccccagcg
     1621 cgcgcagcgc ctgttcgatg cggccgctgg caaacgggtc cgcctggatg ctgaggacgc
     1681 gctctgccag ctccaccacc cgcaccctcg cgccgcgccg gtacagggcc atggccgcct
     1741 ccagccccag gatgcccccg cccaccacca gggcgcgggc gccgggacgg acggcctcca
     1801 ccagggcgct ggcgtcggca aagctgcgca gcacccgcac cgggggcgcg cccgccccgc
     1861 ccttgaccgg gcaaaaggcg ttggccccgg tggcgagcag cagcgccgtg tagggcaaag
     1921 ccctcccatc cgagagcagg gcctcgcggc ggtcggggtc gacgcgctcc acccgggcgt
     1981 cgcagaccag ctcgatccgc cttgcctcgt accagccgta gggctccagc gccaccgact
     2041 cgaaccccgc cccctcggcc acgagccgcg agaggcctgg gcggttataa gggatgatgc
     2101 cctcaccgca gaccagggtc acctttgcgg
//
LOCUS       DBMDYA010000526         2127 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10803_length_2127_cov_1.789733, whole genome shotgun sequence.
ACCESSION   DBMDYA010000526 DBMDYA010000000
VERSION     DBMDYA010000526.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10803_length_2127_cov_1.789733"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="ffh"
                     /locus_tag="ACZPFK_11465"
     CDS             <1..1143
                     /gene="ffh"
                     /locus_tag="ACZPFK_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1406970.1"
                     /translation="ESLTPAQQVIKIVSEELTALMGSESRGIHINAKPPTVVMMCGLQ
                     GSGKTTHSAKIAKYFLKQGHRPLLVACDIYRPAAIDQLKVVGEKAGVPVFELGAERPE
                     VIVKKAYAHARDHGNDLVILDTAGRLHVDDELMAELSRIKEAVPVDETLLVIDAMVGQ
                     DAVNVASSFNEKVGVDGVVLTKLDGDTRGGAALSVRAVTGKPIKFAGVGEKLDDLEVF
                     HPDRMASRILGMGDVMTLIEKAQETQDEKKAQEAAKRLLENKFDMNDLLDQIAQMKKM
                     GGAAAMMSMLPGGAKVSESDLEGSDKMFKRMEAIIQSMTPAERAKPALIDPRRKRRIA
                     AGSGTKVEDVNRILRQYEQMNKLMKQMKKRPKGGRRRFGGMGGMFG"
     gene            1241..1483
                     /gene="rpsP"
                     /locus_tag="ACZPFK_11470"
     CDS             1241..1483
                     /gene="rpsP"
                     /locus_tag="ACZPFK_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1406971.1"
                     /translation="MAVKIRLRRMGAKKNPFYRVVVADSRFPRDGRFIEEIGTYDPSK
                     DPAEVKIDAEKAKKWIANGAQPTDTVRALLKKNEIL"
     gene            1523..1756
                     /locus_tag="ACZPFK_11475"
     CDS             1523..1756
                     /locus_tag="ACZPFK_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1406972.1"
                     /translation="MKELLITLAQGLVEDKDAVKVTADAPDAEGTTVYHLSVAEGDMG
                     RVIGKQGRIAKAIRVVMRAAAVRVGEKVMVEID"
     gene            1866..>2127
                     /locus_tag="ACZPFK_11480"
     CDS             1866..>2127
                     /locus_tag="ACZPFK_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1406973.1"
                     /translation="MQKRYLEAGKFVTTHGVMGELKAYPYCDSAEFLCGFDRLYPAPG
                     GGRPWQVESARAHKGMALLKLEGVDSLDAARPLVNKLFYIDRE"
BASE COUNT          473 a          633 c          706 g          315 t
ORIGIN      
        1 gagagcctga cccccgccca gcaggtcatc aagatcgtca gcgaggagct caccgccctg
       61 atgggcagcg agagcagggg catccacatc aacgcaaagc ctcccacggt ggtcatgatg
      121 tgcggcctgc agggcagcgg taaaaccacc cactcggcca agatcgccaa gtattttctc
      181 aagcagggcc atcggccgct gctggtcgcc tgtgacatct accgtccggc ggccatcgac
      241 cagttgaagg tggtgggtga aaaggcgggg gtgcccgtct ttgagctggg cgccgagcgg
      301 ccggaggtca tcgtcaaaaa ggcctacgcc cacgccagag accacggcaa cgacctggtc
      361 atcctggaca cagccggccg cctgcacgtg gacgacgagc tgatggccga gctgagccgc
      421 atcaaagagg ctgtgccggt ggatgagacg ctgctggtca tcgacgcgat ggtgggccag
      481 gacgcggtca atgtggcctc cagcttcaat gaaaaggtcg gcgtggacgg cgtggtgctc
      541 accaagctgg acggcgacac ccgcggcggc gccgcgctca gcgtgcgcgc ggtcaccggc
      601 aagcccatca aattcgcggg cgtgggcgaa aagctggacg acctggaggt ctttcacccg
      661 gaccgcatgg ccagccgcat cctgggcatg ggcgatgtga tgaccctgat cgaaaaggcg
      721 caggagaccc aggacgaaaa aaaggcgcag gaggcggcca agcggctgct ggaaaataaa
      781 ttcgacatga acgacctgct ggaccagatc gcccagatga aaaagatggg cggcgcggcg
      841 gcgatgatgt cgatgctgcc cggcggcgca aaggtcagcg agagcgacct tgagggcagc
      901 gacaagatgt tcaagcgcat ggaggccatc atccagagca tgacccccgc cgagcgggcg
      961 aagcccgccc tcatcgaccc caggcgcaag cgccgcatcg cggcgggcag cggcaccaag
     1021 gtcgaggacg tcaaccgcat cctgcgccag tacgagcaga tgaacaagct gatgaagcag
     1081 atgaaaaagc gccccaaggg cggccgcaga cgattcggcg gcatgggggg catgttcggc
     1141 tgagcgccgc cgacgcccca cccatcccaa aaggttttta ccgggagacc ggtcaaaata
     1201 cagtttagtt attcaattta tactatggag gaactctatc atggctgtta agatcagact
     1261 gcgccgcatg ggcgccaaga agaacccgtt ttaccgcgtt gtcgtcgccg attcccgctt
     1321 cccgcgcgac ggccgtttca tcgaggagat cggtacctac gaccccagca aggaccccgc
     1381 cgaggtgaag atcgacgctg agaaagcaaa aaagtggatc gccaacggcg cccagcccac
     1441 cgataccgtc cgcgcgctgc tcaaaaagaa cgagatcctg taagcgcagg cacggcataa
     1501 cgcaaagagg taatcaccta tgttgaaaga gcttttgatc accctcgccc agggccttgt
     1561 ggaagacaag gacgcggtga aagtcacggc cgacgcgccg gacgccgagg gcaccaccgt
     1621 ctaccacctg agcgtggcgg agggggatat gggccgcgtg atcggcaagc agggacgcat
     1681 cgccaaagcg atccgtgtgg tgatgcgcgc cgccgccgtg cgcgtgggcg aaaaggtcat
     1741 ggtcgagatc gactgaccgc agttttacag caaaaagagc atgccgggcg cgtccgcagg
     1801 gacctgtccg gcatgttttg ccccgcgggg gataaggcgt ttccacgggg cggaaagggg
     1861 accagatgca gaaaagatac ctggaggcgg gcaagttcgt caccacccac ggcgtgatgg
     1921 gggagctgaa agcctatccc tattgcgaca gcgccgagtt tttgtgcggc tttgaccggc
     1981 tctatcccgc gcccggcggc gggcgtccct ggcaggtgga gtccgcccgc gcccacaagg
     2041 gtatggcgct gctcaagctg gagggggtgg acagcctgga tgcagcgcgc ccgctggtga
     2101 acaagctctt ttacatcgac cgggagg
//
LOCUS       DBMDYA010000527         2126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10812_length_2126_cov_1.811358, whole genome shotgun sequence.
ACCESSION   DBMDYA010000527 DBMDYA010000000
VERSION     DBMDYA010000527.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10812_length_2126_cov_1.811358"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="ACZPFK_11485"
     CDS             complement(<1..227)
                     /locus_tag="ACZPFK_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406974.1"
                     /translation="MTTAMTLERAREAVGQRWRDPGVRGGRQARRRGLRRWFAHHKLA
                     LWLCCYLGVPAAVLGTVCLVCGFLALPALLF"
     gene            468..1610
                     /locus_tag="ACZPFK_11490"
     CDS             468..1610
                     /locus_tag="ACZPFK_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406975.1"
                     /translation="MIEFQNIVKSYKSKTVLNGVSLTVRTGELVALIGASGCGKTTLL
                     KTVNRLGPADSGEVLLDGEPVSRLPLARLRRGIGYVVQDGGLFPHLTVEENLDLVLKN
                     VKLPAAARPARVTELLRMVELPPDEYRALYPCQLSGGQRQRVGVARAFAADPGLILMD
                     EPFSALDPLTRAELQDTVVGLQRRTGKTILFVTHDMDEAVKLAQRICFLEDGRVVQYD
                     TPENVLRSPADEYVADFVGKNRLWGNPEFIKARDIMKKNPSRVSRGRSVLQALQVMNH
                     GGVDSVLVTEGKRLEGVVWLSDLQKFKDYRAPLDEFISQDYISVYEDTSLQKIIDTID
                     YSLSGVLPVISHQGELLGYLTKSILLATLCKQYETPGTQSERSGVI"
     gene            1607..>2126
                     /locus_tag="ACZPFK_11495"
     CDS             1607..>2126
                     /locus_tag="ACZPFK_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1406976.1"
                     /translation="MTLDSFWAAFLDRRGEIWELFLQHIDLSAMAVLLSLALGVPLGL
                     LITKSKPAASLVIGVANVLQSIPSLALLAFLVPFIGIGEKPAILMVVLYALLPIIKNT
                     YTGITGIDPRLLEAADGIGLTRLQRLFRVELPMAAPFIMAGVRISAVGAVGTVTIAAF
                     AGAKGLGWLISLG"
BASE COUNT          379 a          704 c          673 g          370 t
ORIGIN      
        1 aaaaacagca gcgccggcag ggccagaaag ccgcagacaa ggcagaccgt ccccagcacc
       61 gccgcgggca cgcccaggta gcagcacagc cagagcgcca gcttgtggtg cgcgaaccaa
      121 cgccgcaggc cccggcggcg cgcctgccgc ccgccgcgca cacctggatc ccgccagcgc
      181 tgccccaccg cttccctcgc gcgctccagc gtcatcgccg tcgtcatatc cattccctcc
      241 ctgcgggcag cgcccgctct ttttttccac ctccactcta ccatcggccg cataaaacag
      301 gggttagcct tttgccccgg gcgctaaatt tgcgtaaagg cagccgggcc ccgcattctt
      361 aacggaatct ttatctatta aggaattatt ttacgtccgt ctaatcccgc aggccttata
      421 ataggggcaa gcggcggggc aagccgcgcg aaaggatgat ggacgctgtg atcgaatttc
      481 aaaacatcgt caagtcctac aaatcgaaaa cggtgctgaa cggcgtgagc ctgacggtgc
      541 gcaccggtga gcttgtggcg ctgatcggcg cctccggctg cggcaagacg acgctgctca
      601 agaccgtgaa ccggctgggt ccggccgaca gcggggaggt gctgctggac ggcgagcccg
      661 tctcccggct gccactggcc cggctgcggc ggggcatcgg ctatgtggtg caggacggcg
      721 ggcttttccc ccatctgacg gtggaagaaa acctggacct ggtgctcaaa aacgtcaagc
      781 tgcccgccgc cgcccgcccg gcccgggtaa cggagctgct gcgcatggtg gagctgccgc
      841 ccgacgaata ccgcgcgctc tatccctgcc agctgtcggg cggccagcgc cagcgggtgg
      901 gcgtggcccg tgcctttgcc gcagaccccg ggctgatcct gatggacgag cccttttccg
      961 cgttggaccc actgacccgg gctgagctgc aggacaccgt ggtgggcctg cagcggcgca
     1021 ccggcaagac catcctcttt gtcacccacg acatggacga ggcggtgaag ctggcccagc
     1081 gcatctgttt tcttgaggac gggcgggtgg tgcagtacga cacgcccgaa aacgtgctgc
     1141 gcagcccggc ggacgagtac gtggccgact ttgtgggcaa gaaccggctg tggggcaacc
     1201 cggagttcat caaggcccgc gacatcatga aaaagaaccc cagccgcgtc tcccgcgggc
     1261 ggtcggtgct gcaggcgctg caggtaatga accacggcgg cgtggacagc gtactggtca
     1321 ccgagggcaa acggctggag ggtgtggttt ggctgtcaga cctgcaaaaa ttcaaggact
     1381 accgcgcccc gctggacgag tttatctccc aggactatat ctcggtgtat gaggacacca
     1441 gcctgcaaaa gatcatcgac accatcgact acagcctctc gggcgtgctg ccggtcatca
     1501 gccaccaggg cgaactgctg ggctatctga ccaaaagcat tctgctggcc accctgtgca
     1561 agcagtacga gacgcccggc acccagagcg aaaggagcgg cgtgatatga cactggacag
     1621 cttttgggcc gcgtttctcg accggcgcgg cgagatctgg gagctctttt tgcagcacat
     1681 cgacctgtcc gccatggccg tgctgctctc gttggcgctc ggtgtgccgc tgggcctgct
     1741 catcaccaaa agcaagcccg ccgcctcgct ggtcatcggc gtcgccaacg tgctgcagtc
     1801 gatccccagc ctggcgctgc tggcttttct cgtgcccttt atcggcatcg gcgaaaagcc
     1861 cgctattttg atggtggtgc tctacgcgct gctgccgatc atcaaaaaca cctacaccgg
     1921 catcaccggc atcgacccca ggctgctgga ggcggcggac ggcatcggcc tcacccggct
     1981 gcagcggctg ttccgggtgg agctgcccat ggccgcgcct ttcatcatgg ccggcgtgcg
     2041 catctcggcc gtgggcgcgg tgggcaccgt gaccatcgcc gcctttgccg gggccaaggg
     2101 gctgggctgg ctgatcagcc tgggtc
//
LOCUS       DBMDYA010000528         2125 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10822_length_2125_cov_1.013340, whole genome shotgun sequence.
ACCESSION   DBMDYA010000528 DBMDYA010000000
VERSION     DBMDYA010000528.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10822_length_2125_cov_1.013340"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="ACZPFK_11500"
     CDS             complement(<1..235)
                     /locus_tag="ACZPFK_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1406977.1"
                     /translation="MPTFNQLVRKGREVMVKKSTAPALLRGYNSQKKQYTEQNSPQKR
                     GVCTAVRTMTPKKPNSALRKVARVKLTNGIEVTS"
     gene            complement(689..>899)
                     /locus_tag="ACZPFK_11505"
     CDS             complement(689..>899)
                     /locus_tag="ACZPFK_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406978.1"
                     /translation="SLATDSFLSAASFQETTRVLTEAAIKGKVDPLLGLKENVIIGKL
                     IPAGTGLGEEEAAAFEAEESSPEVG"
     assembly_gap    900..909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<910..>2125)
                     /locus_tag="ACZPFK_11510"
     CDS             complement(<910..>2125)
                     /locus_tag="ACZPFK_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1406979.1"
                     /translation="FMMSNSGARGSMNQIRQLAGMRGLMANTAGKTIEIPIKANYREG
                     LNILEYFISSRGARKGLADTALRTADSGYLTRRLVDVSQDVIVREEDCGTTEGIWVED
                     IRVGNELYEPLDERLLGRYPVSDILDPETGALLVSKDTMMHEEEAKKVIEAGIKKVHI
                     RSVLGCRAQHGCCAKCYGANLANGRPVTIGEAVGIISAQSIGEPGTQLTMRTFHTGGI
                     ANAEDITQGLPRVEELFEARRPKSLAIMTEIAGTVHVDDTKKNRHIVVSGVDENGAPV
                     EKSYMIPFGQRVRVAEGDVLELGDMVTEGNAYPQDILAIKGVAATEDYIIQQVQQTYR
                     MQGVDINDKHIEVIVHQMMRKARVDDAGSTSLIGGALADKQEIEAKNAEIRRRIADGE
                     TDLKEAKYTTVLL"
BASE COUNT          353 a          646 c          615 g          501 t
ORIGIN      
        1 aagaagtgac ctcgatacca tttgtgagct tgacacgagc aaccttgcga agtgcggagt
       61 tcggcttttt gggggtcatg gtgcgcaccg cggtgcagac gccgcgcttt tgcggggagt
      121 tctgctcggt gtactgcttc ttctggctgt tatagccgcg gagcagcgcg ggagcggtgg
      181 actttttcac catcacctca cggcctttgc ggacgagctg gttaaaggtg ggcatgattt
      241 tcctcctttc ctgtgaaaaa tttttgaggg caaaagccct ctctatgttg tggcggcaag
      301 ccgccggaca accgtaaaac aggcgcagag caacgcaaaa cacgcccttg gcgcaacatt
      361 tatattttac acgtttcttt ttgcgctgtc aacagaaaag ccgcgtaaaa ttcagcattt
      421 tgccgatttt tcagcgctgc ggtatccttg tccgcaaaca tgccgacaac agatatagtt
      481 taccattttc aggaatctca tacaatcttt tttaaaaatt ttgcgtgcgg ttttttcttg
      541 cccggtatgg ctttttattg cctgcgcttt ccccttttct ttttttcatt ttcatcgccc
      601 ttttttctgg tgcgaccctg caaaaaagag ggcagcccaa aggccgccct cttttttcgt
      661 tttactcttt taagttttgc atcctatatc agccgacctc ggggctggat tcctccgcct
      721 cgaacgcggc ggcctcctct tcgcccaggc cggtgcccgc ggggatcagc ttgccgatga
      781 tgacgttctc tttcaggccc agcagcgggt cgaccttgcc cttgatggcg gcttcggtca
      841 gcacgcgggt ggtctcctgg aaagaggctg cggacaggaa cgaatcggtg gccagcgagn
      901 nnnnnnnnnc cagcagcacc gtggtgtact tggcctcttt caggtcggtc tcgccgtccg
      961 caatgcggcg gcggatctcg gcgttcttcg cctcgatctc ctgcttgtca gccagcgcgc
     1021 cgccgatcag gctggtggag cccgcatcgt ccacccgggc cttgcgcatc atctgatgca
     1081 cgatgacctc gatgtgcttg tcgttgatgt caacgccctg catgcggtag gtctgctgca
     1141 cctgctggat gatatagtcc tcggtggcgg ccacgccctt gatcgccagg atatcctgcg
     1201 ggtaggcatt gccctcggtg accatgtcgc ccagctccag cacatccccc tctgccacac
     1261 gcacgcgctg gccgaacggg atcatatagc tcttctccac cggcgcgccg ttctcatcca
     1321 caccggagac cacaatatgg cggtttttct tggtgtcgtc cacgtgcacc gtgcccgcga
     1381 tctcggtcat gatggccagg gatttgggcc gccgggcctc gaacagctcc tcaacgcggg
     1441 gcaaaccctg ggtgatatcc tcggcgttgg cgatgccgcc ggtgtggaac gtgcgcatgg
     1501 tcagctgggt gccgggctcg ccgatggact gggccgaaat gatgcccacg gcctcgccga
     1561 tggtgaccgg gcggccgttg gccaggttgg cgccgtagca cttggcgcag cagccatgct
     1621 gcgcgcggca gcccagcaca ctgcggatgt gcaccttttt gatgcccgcc tcgatgacct
     1681 ttttggcctc ttcctcatgc atcatggtat ccttggaaac cagcagcgcg ccggtctccg
     1741 gatcgaggat atcgctgacc ggatagcggc ccagcagccg ctcgtccagc ggctcgtaca
     1801 gctcgttgcc cacccggatg tcctccaccc agatgccctc ggtggtgccg cagtcctcct
     1861 cgcggacgat gacgtcctgc gagacgtcca ccagacggcg ggtcagataa cccgagtcgg
     1921 cggtgcgcag cgcagtgtcg gccaagccct tgcgggcgcc gcggctggag atgaagtact
     1981 ccaggatgtt caggccctcg cggtagttgg ccttgatggg gatctcgatg gtcttgccgg
     2041 cggtgttggc catcaggccg cgcatgcccg ccagctggcg gatctgattc atactgccgc
     2101 gggcgcccga attggacatc ataaa
//
LOCUS       DBMDYA010000529         2123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10830_length_2123_cov_1.605836, whole genome shotgun sequence.
ACCESSION   DBMDYA010000529 DBMDYA010000000
VERSION     DBMDYA010000529.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10830_length_2123_cov_1.605836"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..440)
                     /locus_tag="ACZPFK_11515"
     CDS             complement(108..440)
                     /locus_tag="ACZPFK_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406980.1"
                     /translation="MAATKIAWTKVPLNGGVALPALAALSAEGGEIAFDGQDTKTLIL
                     VENSASSAGEITFKAGSGIQGVADLTVSVPASTTQAFVLESGAFKSKGVVKVTGATTM
                     KVGALLLP"
     gene            complement(1114..1950)
                     /locus_tag="ACZPFK_11520"
     CDS             complement(1114..1950)
                     /locus_tag="ACZPFK_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406981.1"
                     /translation="MAYQNFIPDVWSTAINRELERSLVYAEGCNRQYEGDVKKMGDAV
                     RILGVGKPTITTTENKEIVLDDPEKVDDTSVLLPIRQISYFNYRVDDIDKRQATGGVL
                     EALSKEATQGLAFAMDTHIAGVAAAREALKYSDAAVAITKDNVLEEIDKALEKLYEND
                     VRPNGKIMMEIPPWLYMRLKQAYTVLDTDNSKLLENGRVGRYGNVIVKMSNNVARDKE
                     GNSLVMVHTDKAIAFVNPMTHTEAYRPEKGFSDAVKGFVLYQAKIVRPKELMVMNVKA
                     GA"
BASE COUNT          334 a          688 c          554 g          547 t
ORIGIN      
        1 tctgattttg tggccgtcca tgcgagaacg gcggggccgt tctcctcagc atctacttcc
       61 gcttccgtga ccggctatgg ctttctcgcc ccaccggtct tattctttta cggcagcaaa
      121 agcgcgccca ctttcattgt ggtggcgccg gtcactttta ccacgccctt gctcttgaat
      181 gcgccgctct ccagcacaaa ggcctgcgtg gtggaggcgg gcacgctcac cgtcagatcc
      241 gccaccccct ggatgccgct gcccgcctta aaggtgattt ccccggcgct ggaagcgctg
      301 ttctccacca ggatcagggt cttggtgtcc tgcccgtcaa aggcgatctc gcccccctcc
      361 gcgctcagcg ccgccagggc gggcagggca acgccgccgt tcagcggcac tttcgtccat
      421 gcaatttttg ttgcagccat ctctcattcc tcactttctc cgggtcacgc cccggctttc
      481 acgttcattt atgtccgttc tttgatcttt ctcaaaccgc gcaccagctc ctgcgtgttc
      541 taagcgcaag cggaacacgc gcagatcatg aggcgttaaa aacacgacgg attagtcccc
      601 ggtttttcaa agctgtactg ctttgaaaaa gccgtgggcc tctgttccgg cgtgtttagc
      661 cgaatgacct atagccacgg gtccgccgca ttggctgcag accttattgt aacctttggt
      721 gttaccggcc aatgctgcga ccttgctgcg caaggtccgt ttctttgatc tttctcaaac
      781 cgcgcaccag cccctgcgtg ttctaagcgc aagcggaaca cgcgtagatg atgaggcgtt
      841 aagaacacga cggattagtc cccggttttt caaagctgta ttgctttgaa aaatccgtgg
      901 gcccctgttc cgacgtgttt agccgaatga cctctagcca cgggtccgct gcgtggcaaa
      961 cagcgggacc ttttggttcc ttttcggtca cgaaaaggaa ctcatcgaag attttaaaat
     1021 gtttttttcc tctccgtcgc aaggtccatt tttgcgctct tttcttccgc ttccgtgcgg
     1081 cccgatggca acgacgcttt tccgcgctct cctttacgcc ccggctttca cgttcatcac
     1141 catcagttcc ttgggtctta ctatctttgc ctgatagagc acaaagccct ttaccgcgtc
     1201 tgaaaagccc ttttccgggc ggtaggcctc ggtgtgggtc atggggttga caaaggcgat
     1261 ggccttgtcg gtgtgcacca tcaccaggct gttgccctcc ttgtccctgg ccacgttgtt
     1321 gctcatcttg acgatcacgt tgccgtagcg gcccacgcgg ccgttctcca gcagcttcga
     1381 gttgtcggtg tccagcacgg tgtaggcctg tttcagccgc atgtacagcc agggcgggat
     1441 ctccatcatg atcttgccgt tgggccgcac gtcgttttcg tacagctttt ccagcgcctt
     1501 gtcgatctcc tccagcacgt tgtccttggt gatcgccacc gcggcgtcgc tgtatttgag
     1561 cgcctcccgg gccgcggcca cgcccgcgat gtgggtgtcc atggcaaagg ccagcccctg
     1621 ggtggcctcc ttgctcagcg cctccagcac gccgccggtg gcctgccgct tgtcgatgtc
     1681 gtccacccgg tagttgaaat agctgatctg ccggatgggc agcagcacgc tggtgtcgtc
     1741 caccttttcg gggtcgtcca gcacgatctc cttgttctcg gtggtggtga tagtgggctt
     1801 gcccacgccc aaaatgcgca ccgcgtcgcc catctttttc acgtcgccct catactgccg
     1861 gttgcagccc tccgcgtaga ccagcgagcg ctccagctcc cggttgatcg ccgtggacca
     1921 gacgtccggg ataaaattct gatatgccat ctgttgaaac tcctcctttt tttgaacggc
     1981 aagccgttct tccatctgtt tctttccgct ttcagccgtc ggccgcccat tttcgccgcc
     2041 gtccgcacgg gaacaaacgg ccgttctgcc cgcgttccct ctcggacggt tttaaaagcc
     2101 gacccgtccc cgttttcggc agc
//
LOCUS       DBMDYA010000530         2120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10843_length_2120_cov_1.572561, whole genome shotgun sequence.
ACCESSION   DBMDYA010000530 DBMDYA010000000
VERSION     DBMDYA010000530.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10843_length_2120_cov_1.572561"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(145..966)
                     /locus_tag="ACZPFK_11525"
     CDS             complement(145..966)
                     /locus_tag="ACZPFK_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1406982.1"
                     /translation="MKQVVRRPCFLRQIRRRMTNCYWEIGEAWMKQRGLKIIKLLAMT
                     LVPSGVFTAFYIASGWFWRGIPSLALFCVVAVPTLFLFEMVVVLYANKKEYGSYGVQI
                     AFTENEKTVWWKTFFYGFLLFSFAGLMTVTIAPLENTLMAEAFQKINSILPAYFDWNN
                     FELMKQYSKGTLLFTSVLYIITNVFIGPIIEEIYFRGYLTSRLKDFGIAAPILITIVF
                     SLYHWWLSFNNVFRICAFTVAAVIAYRKKNIYITMVFHCLCNLFSSISFIMTLWG"
     gene            complement(1527..>2120)
                     /locus_tag="ACZPFK_11530"
     CDS             complement(1527..>2120)
                     /locus_tag="ACZPFK_11530"
                     /inference="COORDINATES: protein motif:HMM:NF018816.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1406983.1"
                     /translation="NQRGEHFMQNRASFNVRRLAVTGLLAALVFIFSWMQIPIGQIAR
                     IHLGNVFCALSGLLFGPLTGGLASGFGSMLFDLTNPAYIAECWITFITKFFIGFLAGL
                     IAGRGEKQGLGRDVLGAAVGSVAYVALYLVKSFIVMTIEGQAQGAIVTQLYTKGATSL
                     TNAVMAVIASVLLARALRPALRRAGILPLQDGRAAKG"
BASE COUNT          574 a          526 c          519 g          501 t
ORIGIN      
        1 ttatttgaat ggacctgttt cccgccgctg ctgcagcaac cgagacggct ggccgaaaaa
       61 agaagctgcg gccatggaaa acggtaaaac gagacatgca catagtccga taaggcgaat
      121 gagttcggac cgacaaacgg gaaatcaacc ccacaaagtc attataaaac taatagatga
      181 aaataaattg cacaggcagt ggaataccat tgtaatatat atgttctttt ttctgtaagc
      241 gattaccgca gccactgtga atgcacaaat tctaaagacg ttgttaaagg aaagccacca
      301 gtgatacaac gaaaatacaa tcgtaataag gataggagcg gcaattccaa agtcttttaa
      361 tctgcttgtc agatatcctc taaaatagat ctcctctata attgggccta tgaatacgtt
      421 cgttataata taaaggacac ttgtaaatag gagtgttcct ttagaatact gcttcataag
      481 ttcaaaattg ttccagtcaa agtatgccgg taaaattgaa ttgatttttt ggaaagcttc
      541 cgccatcagc gtattttcaa gcggtgcaat cgttacagtc ataagacctg caaagctgaa
      601 caataaaaaa ccatagaaaa acgttttcca ccaaacagtt ttttcattct ctgtaaaggc
      661 tatttgaact ccatagcttc catactcctt tttattggca tacaaaacga caaccatctc
      721 aaacaagaac aaagtcggaa ctgctacaac acaaaataga gcgagagagg ggatccctct
      781 ccaaaaccac cctgaagcta tgtagaaagc agtaaaaact ccacttggca caagcgtcat
      841 tgctaataac tttataatct taaggcctct ttgcttcatc catgcctctc caatctccca
      901 atagcaattt gtcattctgc gccttatttg ccttaagaag catggacgcc ttaccacttg
      961 tttcattcta tgatttcagt gtgattctgt caaatataga aatattgatt taaaatttct
     1021 cgtaagctat tgttttgttt gggcgccaat cgggcgccat tctgccgtct tgttcaacca
     1081 tccatacttc tgtaaaattg acagctgaat aaagttgagt catcactgct tttcattgct
     1141 ttgcaataaa aattatcttg atgactccgg cccgaggacg ggccggatgg ctgaaaaaac
     1201 agtctccgct tcgctgcgac tgtttttcat tgctgcgcaa taaaaaacaa cttatcttga
     1261 tagccccggc ccgaggacgg gccggatggc tgaaaaaaca gtctccgctt tgctgcgact
     1321 gttttttggg cttcccggga cgagaaaata agaaagcgcg ccttgaaaaa gaggagaggt
     1381 cttttcaaag cgcgctttct gtgtttagga ggagacccct gcggctgacg tcgggtccgg
     1441 cgttcgccgc tgccgtactg tttttcagac cgcaggaggc ggccgcaaga cggggcaggg
     1501 cgaaagtatc caccctattt gtgtctttat ccctttgcgg cgcgcccgtc ctgcagcggc
     1561 aggatgccgg cccggcgcag ggcggggcgc agggcacgcg ccagcagcac cgaggcgatc
     1621 acagccatca cggcgttggt cagggaggtg gcgcccttgg tgtagagctg ggtgacgatg
     1681 gcgccctggg cctggccctc gatggtcatg acgataaagg acttcaccag gtagagcgcc
     1741 acgtaggcca cactgcccac cgccgcgccc agcacgtccc ggccgaggcc ctgcttttcg
     1801 ccgcggcccg cgatcaggcc ggcgagaaag ccgatgaaaa atttggtgat gaaagtgatc
     1861 cagcactcgg cgatgtaggc cgggttggtc aggtcgaaca gcatgctgcc gaagccggag
     1921 gcaagcccgc cggtgagcgg accgaacaac agccccgaca gggcgcagaa cacgttgccc
     1981 agatggatgc gggcgatctg gccgatgggg atctgcatcc agctgaagat gaagaccagg
     2041 gccgcgagca gcccggtgac cgccagacgg cgcacgttga aagaagctct gttttgcata
     2101 aaatgttccc ctctctgatt
//
LOCUS       DBMDYA010000531         2118 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10852_length_2118_cov_1.642043, whole genome shotgun sequence.
ACCESSION   DBMDYA010000531 DBMDYA010000000
VERSION     DBMDYA010000531.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_2118_cov_1.642043"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            160..591
                     /locus_tag="ACZPFK_11535"
     CDS             160..591
                     /locus_tag="ACZPFK_11535"
                     /inference="COORDINATES: protein motif:HMM:NF017984.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR1406984.1"
                     /translation="MDASAYPEAGEELELEPVRLQGKTRYLLASERQAFDTGDGEGQA
                     LLLLGPHDPYLDLRDRAVVLPDPALQRRVWKTAANPGAVLKGGRIVGVWQAKAAGGGL
                     DARLSVWEALTAAERRSLEQRAEEYAAFRRLPLRGFALEEG"
     gene            691..1566
                     /locus_tag="ACZPFK_11540"
     CDS             691..1566
                     /locus_tag="ACZPFK_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1406985.1"
                     /translation="MQIELKRGGAAALVDTHGAELFSYLTPEKEQALWGGSPDSWPGR
                     SPVLFPLIGAVKGRILRIDGRQTPVKNHGFGRDLDYAVIARSDVSVTLRAQADEATLA
                     VYPYPFALEITHALTGAGFVTRYRVINTGSRPLSYCIGGHVGFCCPRGAGAFEDYDLL
                     LPERMDVRLMPAQPGPLPGQQPSRLLFSQVDGFGLDRALFDQGTLYLEQPALHSITLR
                     DRRSGRGVSMSYAGFPVMALWTKERAGAPFLCIEPWHGLPEMEGESGLFEEKPYRIPL
                     APGESRALEYAVAVF"
BASE COUNT          358 a          708 c          715 g          337 t
ORIGIN      
        1 cgggaatctg tggagtcggt acaggaggca gaaatatctc aggaagaaat gatattgggg
       61 acagcggggg cagaaacagc tgaaactgcg gagactcagg aggagcaggc tgacagcgta
      121 ccggaaacca atggggagac agcggaagtt gtaaatcaga tggatgcttc agcgtatccg
      181 gaggccgggg aggagttgga gctggaaccg gtgcggctgc agggcaagac ccgctatctg
      241 ctcgcctccg agcggcaggc tttcgacaca ggggacggcg aggggcaggc gctgcttttg
      301 ctggggcccc acgaccccta tctggacctg cgggaccgtg cggtggtgct gccggacccc
      361 gctctgcagc ggcgggtctg gaagacggcg gcgaacccgg gcgcggtgct gaagggcggc
      421 cggatcgtcg gcgtctggca ggccaaggcg gccgggggcg ggctggatgc ccgcctctcg
      481 gtgtgggagg cgctgacggc ggcggagcgc cgcagtctgg aacagcgggc ggaggaatac
      541 gccgctttcc gccggcttcc cctgcgcggc tttgcgctgg aagagggctg acccgcaccg
      601 gcgcaccggc cggcccggcg ggcgctgtaa aaaacggaaa agccggcggc ccaccgttgc
      661 cgccgacaag agatttaagg agggatcgcc atgcagatcg aattgaaacg gggcggcgcc
      721 gccgccctcg tcgacaccca cggggcggag ttgttctcct acctcacccc ggaaaaggag
      781 caggcgctct ggggcggcag cccggacagc tggcccgggc gcagcccggt gctgttcccg
      841 ctgataggcg cggtaaaggg ccgcatcctg cgcatcgacg ggcggcaaac cccggtcaaa
      901 aaccacggct tcggccgcga tttggactac gccgtcattg cccgcagcga cgtctcggtc
      961 accctgcgcg cccaggccga cgaggccacg ctggccgttt atccctatcc ctttgcgctg
     1021 gagatcaccc acgccctcac cggcgcgggc tttgtcaccc gctaccgggt gatcaatacc
     1081 ggcagccggc cgctgtccta ctgcatcggc ggccatgtgg gcttttgctg cccgcggggc
     1141 gcgggcgcct ttgaggacta tgacctgctg ctgccagagc ggatggacgt gcggctgatg
     1201 cccgcccagc ccgggccgct gcccggccag cagcccagcc gtcttttgtt ctcgcaggtg
     1261 gacggcttcg ggctggaccg cgccctcttc gaccagggga ccctttacct ggaacagcct
     1321 gcgctgcaca gcatcacgct gcgggaccgc cgcagcggcc gcggcgtgtc catgagctac
     1381 gccgggtttc cggtgatggc cctgtggacc aaagagcggg cgggcgcgcc ctttctctgc
     1441 atcgagccct ggcacggcct gcccgagatg gagggcgaga gcggcctgtt tgaagaaaag
     1501 ccctaccgca tccccttggc ccccggcgag agccgcgcgc tggaatacgc ggtggccgtc
     1561 ttctgagacc cgctcccgca gggcccgtcc ggcgcaaaaa gcagcggatt ccgctccgca
     1621 ggccaggacg cttcacaaac taaaaaaggt tggcgatctc tgtgaatcgc caaccttttt
     1681 tgaatttgtt cccctttcaa atcgccgggc caaagctgcc gcacgtcccc aaacaggaaa
     1741 gccgcaattc ccagccggaa gaatcccggc agctcccttt ccgggcgctg agcgggtctg
     1801 agccgccctg cgcacggatt cctcgcctta agcccatatc ccctgcgaaa acgccccttt
     1861 gagagcgttt tttgtctcaa accacattgt ccaaccgcca gtcggaccgt ctcacctcgc
     1921 ccgcgtcctg tccagccgtg ccaaagccgg gcctctgcac gtgcggtttg agacaaaccg
     1981 gcccggccgc gcccgctgcg ctcatcctcc tcgggttgcc agatacgcgg agatggcggc
     2041 ggcggctttt ttgcccgcgc ccatggcgga gatgacggtg gcggcgccgg tcaccgcgtc
     2101 gccgcccgcc cagacgcc
//
LOCUS       DBMDYA010000532         2111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10898_length_2111_cov_1.613930, whole genome shotgun sequence.
ACCESSION   DBMDYA010000532 DBMDYA010000000
VERSION     DBMDYA010000532.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_2111_cov_1.613930"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="ACZPFK_11545"
     CDS             <1..782
                     /locus_tag="ACZPFK_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR1406986.1"
                     /translation="GDIARLHEAVLAYARDFMIDKKGFTYVIPPFMMHGSVVQGVMSF
                     PEMDAMMYKIEGEDLYLIGTSEHTMIGRFVDQIIPEDQLPQTLTSYSPCFRKEKGSHG
                     IEERGVYRIHQFEKQEMIVVCKPEDSMNWYEKLWKNSVELFRSLDIPVRQLECCSGDL
                     ADLKVKSCDIEAWSPRQKKYFEVCSCSNLGDAQARRLRIRIKGADGKNYLAHTLNNTC
                     VAPPRMLIALLENNLQADGSVKIPAVLQPYMGGKTVLTPKK"
     gene            959..1035
                     /locus_tag="ACZPFK_11550"
     tRNA            959..1035
                     /locus_tag="ACZPFK_11550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:993..995,aa:Met,seq:cat)
     gene            1119..1193
                     /locus_tag="ACZPFK_11555"
     tRNA            1119..1193
                     /locus_tag="ACZPFK_11555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1152..1154,aa:Glu,seq:ttc)
     gene            1785..>2111
                     /locus_tag="ACZPFK_11560"
     CDS             1785..>2111
                     /locus_tag="ACZPFK_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR1406987.1"
                     /translation="MALIQVNFISNTLRRTVPLQVILPVDKGDGNRFLNDPQRRYPTL
                     YLLHGLFGNCTDWVSNTRVQMWAEEKNLAVVMPSGDNSFYVDQPGPNSDYGAFIGEEL
                     VELTRRM"
BASE COUNT          499 a          571 c          666 g          375 t
ORIGIN      
        1 tgggggatat cgcccgcctg cacgaggcgg tgctggccta tgcgcgcgac tttatgatcg
       61 acaaaaaagg gttcacctat gtcatccccc cttttatgat gcacggctcg gtggtgcagg
      121 gggtgatgag cttccccgag atggacgcca tgatgtacaa gatcgagggg gaggatctct
      181 acctgatcgg cacctccgag cacacgatga tcggccgttt tgtggatcag atcatccccg
      241 aggaccagct gccgcagacc ttgaccagct acagcccttg cttccgcaaa gaaaagggca
      301 gccatggcat tgaggagcgg ggcgtttacc gcatccatca gtttgaaaag caggagatga
      361 tcgtggtctg caagccggag gacagcatga actggtatga aaagctgtgg aaaaacagcg
      421 tggagctgtt ccgcagcctg gatatcccgg tgcgccagct ggaatgctgc agcggcgatc
      481 tggcggacct gaaagtcaag agctgcgata tcgaggcctg gagcccgcgg cagaagaagt
      541 attttgaggt ctgcagctgc tccaacctgg gggatgccca ggcacggcgg ctccgcatcc
      601 gcatcaaggg cgcggacggc aagaactatc tggcccatac cctcaacaac acctgcgtgg
      661 cgcccccgcg catgctgatc gccctgctgg agaacaatct gcaggcggac ggcagtgtaa
      721 agatcccggc ggtgctgcag ccgtatatgg gcggcaagac cgtgctgacg ccgaaaaagt
      781 aaagttgaat ggaaaaatat ggaaaaccgg gtggagaact ctccgcccgg ttttttcaaa
      841 aaaaaggggc aaactaacag ggacaaaaaa ccgcgatacg accgcaaaaa aggttgacaa
      901 gccgccaaaa acccgatata atatcaaatg caccattcat aaatggcttg ccaaaaatgg
      961 cggtatagct cagttggcta gagcattcgg ttcatacccg aagtgtcgta ggttcgagtc
     1021 ccactaccgc taccagcaca aagtttaggt aaaccagtag ggtaccgctt gcaacagcgg
     1081 gtggtgtgag gctttacgat tttacctaag ctttgcacgg cccgatggtc aagcggtcaa
     1141 gacaccaccc tttcacggtg gtatcccggg ttcgattccc ggtcgggtca ccaaaaacgc
     1201 cccgccgaca ggcggggtct ttttttgtgg ccaccgggtg agaagccggg acaagcgcag
     1261 gggagagtga gggagaacgg cagtgccgtt ctcgccgggc tggccgaaaa gacgggcagc
     1321 ggagagacaa gcttgcagcc ggccccagat tcccggtcgg gtcaccaaaa aacgccccgc
     1381 cgacaggcgg ggtctttttt tgtgcccacc gggtgagaag ccgggacaag cgcaggagaa
     1441 agcaggggag aacggctgcg tcgttctcgc tgggccggcc gaaaagacgg gcagcggaga
     1501 gacaaccgtg agggaaagga acggcagcgc cgatgggccg cggcaatgcc ttgtgcctat
     1561 cgcaggacat aaaaagagag tattggacaa atttccgctt tccgctacaa taaaggggag
     1621 ggcgtaaaag cgtcacttcg ccccgcctgt agggcgtgga gcggtgcgca ggcctgcgcg
     1681 ccgggcgcat ggcgcttttg ctccaccggg ccctgccggg ccgcgcgggc gaaaccggcc
     1741 gggatcgcgg cagaaaatga tttgaaatag gggaggtttc gaccatggcg ctcatccagg
     1801 taaattttat ctccaacacg ctgagacgga ccgtaccgct gcaggtgatc ctgccggtgg
     1861 acaaggggga cggcaaccgc tttctcaatg acccgcagcg gcggtatccc accctttatc
     1921 tgctgcacgg cttgttcggc aactgcaccg actgggtcag caatacccgc gttcagatgt
     1981 gggcggagga aaagaatctg gccgtggtca tgcccagcgg ggacaactct ttttatgtgg
     2041 accagcccgg gcccaacagc gattacggcg cctttatcgg ggaagagctg gtggagctga
     2101 cccgcaggat g
//
LOCUS       DBMDYA010000533         2110 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10905_length_2110_cov_1.450473, whole genome shotgun sequence.
ACCESSION   DBMDYA010000533 DBMDYA010000000
VERSION     DBMDYA010000533.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10905_length_2110_cov_1.450473"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="ACZPFK_11565"
     CDS             <1..732
                     /locus_tag="ACZPFK_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1406988.1"
                     /translation="VVGGILLGLAANLVMALIVGFVGSKLSGLYFTMALIALHAIIYT
                     LAGQLSFTGYALGLSMPREYLMSRHALYFLVLGLAALMMLIFVWVRKSRIGTNFVALK
                     ENPDLAVSLGSNIRGWRILAVIISAMMASIMGAFYAFYMMANTPEVFASTISLRIIMV
                     CMVGGLGNVWGPVMGCWLIVFDELVRGIMPSKFASFAVVVYALVLIVMALLKPKGIMG
                     SKIFQPKETDARRVEHKKAGAKAQK"
     gene            749..1489
                     /locus_tag="ACZPFK_11570"
     CDS             749..1489
                     /locus_tag="ACZPFK_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406989.1"
                     /translation="MFFRVENSTIAFGGLVANKDVNVEVEKGQIVGLIGPNGAGKSTL
                     FKSISGFNKIDSGHIWFDGREITNLEPNHVCKAGIACTFQKAQSFADMTLEESVLVGA
                     YCRRKSKKEALAYAREMIGFVGLAEKQNVKISKLNMYERKKAELAAALATEPKMLLLD
                     ELFAGLVPTEVGYILEYVKKVNRELGITLLIVEHVLKVIMSLCDKIYVLEYGQIIASG
                     SPAEVTSNPKVIKAYLGDDYDVTADQQP"
     gene            1461..>2110
                     /locus_tag="ACZPFK_11575"
     CDS             1461..>2110
                     /locus_tag="ACZPFK_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1406990.1"
                     /translation="MMLLQISNLEVVLGGLQILQDVSLGVNEGELVVVVGANGAGKST
                     LLRTVCGMNPCAAGKVEFNGKDITNQASHDGAGEGLCMVPEGRQLFPELTVRENLLMG
                     AYHYRKDKKRVLDNLERCYAMFPVLKKYEGRIASTFSGGEQQMISIARGLMSEPKIMM
                     IDELSLGLAPLVVEELFKTIRQLNATGMSILLIEQNARQALRIADRAYVLESGKIT"
BASE COUNT          429 a          612 c          665 g          404 t
ORIGIN      
        1 gtggtgggcg gcatcctgct gggcctcgcc gccaacctgg tgatggcgct gatcgtcggc
       61 tttgtgggct caaagctctc ggggctgtat tttacgatgg ccctcatcgc cctgcacgcc
      121 atcatctaca cgctggccgg gcagctgtcg ttcaccggct acgcgctggg cctgtccatg
      181 ccgcgcgagt acctgatgag tcgccacgcc ctctactttt tggtgctggg gctggccgcg
      241 ctgatgatgc tcatctttgt ctgggtgcgc aaaagccgca tcggcaccaa ctttgtcgcc
      301 ctcaaggaaa acccggacct ggccgtctcg ctgggctcca acatccgcgg ctggcgcatc
      361 ctggcggtga ttatcagcgc catgatggcc tctatcatgg gcgcgttcta cgccttttac
      421 atgatggcaa acacccccga ggtgttcgcc tccaccatct cgctgcgcat catcatggtc
      481 tgcatggtgg gcggcctggg caatgtctgg ggcccggtca tgggctgctg gctgatcgtc
      541 tttgacgagc tggtgcgcgg catcatgcca agcaaattcg cctcttttgc ggtggtcgtc
      601 tacgcgctgg tgctcatcgt gatggcgctg ctcaagccca agggcattat gggcagcaag
      661 atttttcagc ccaaggagac cgacgcccgc agggtggaac acaaaaaggc cggcgcaaag
      721 gcacaaaaat aagggaggag gtaccgcgat gttttttagg gtggagaatt cgaccatcgc
      781 gttcggcggc cttgtggcga acaaggacgt gaacgtcgag gtggaaaaag gacagatcgt
      841 gggcctcatc gggcccaacg gcgcgggcaa atccacgctg ttcaaatcca tttccggctt
      901 caacaagatc gacagcggcc acatctggtt cgacggcagg gagatcacca acctggagcc
      961 gaaccacgtg tgcaaggcgg gcatcgcctg cacgtttcaa aaggcgcagt cctttgccga
     1021 catgacgctg gaggaatcgg tgctggtggg cgcctactgc cgccgcaaga gcaaaaaaga
     1081 ggctctggcc tacgcccgcg agatgatcgg gtttgtgggc ctggccgaaa agcagaacgt
     1141 gaagatctca aaattgaata tgtacgagcg caaaaaggcc gagctggccg ctgcgctggc
     1201 caccgagcct aagatgctgc tgctcgacga gctgttcgcc ggcctggtgc ccaccgaggt
     1261 gggctatatc ctggaatatg tcaaaaaggt caaccgggag ctgggcatca cgctgctcat
     1321 cgtcgagcac gtgctcaagg tgatcatgag cctgtgcgac aagatctatg tgctggagta
     1381 cggccagatc atcgccagcg gcagcccggc ggaggtcacc agcaacccca aggttatcaa
     1441 agcttattta ggagatgatt atgatgttac tgcagatcag caaccttgaa gtggtgctgg
     1501 gcggccttca gatcctgcag gacgtcagcc tgggggtcaa cgagggggag ctcgtcgtcg
     1561 tggtcggcgc caatggggcg ggcaaatcca ccctgctgcg caccgtctgc ggcatgaacc
     1621 cctgcgccgc cggcaaggtg gagtttaacg gcaaggacat caccaaccag gcctcccacg
     1681 acggggcggg cgaggggctg tgcatggtgc cggaggggcg gcagctcttc cccgagctga
     1741 ccgtacgcga aaacctgctg atgggggcct accattaccg caaggacaaa aagcgggtgc
     1801 tggacaatct ggagcgctgc tacgccatgt tcccggtact gaaaaaatac gagggccgca
     1861 tcgccagcac cttttcgggc ggcgagcagc agatgatctc catcgcccgc ggcctgatga
     1921 gcgagcccaa aatcatgatg atcgacgagc tgtcgctggg ccttgcgccg ctcgtggtgg
     1981 aagagctgtt taagaccatc aggcagctca acgccaccgg catgagcatc cttttgatcg
     2041 agcagaacgc ccggcaggcg ctgcgcatcg ccgaccgcgc ctatgtgctg gagagcggta
     2101 agatcaccat
//
LOCUS       DBMDYA010000534         2110 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10907_length_2110_cov_1.237929, whole genome shotgun sequence.
ACCESSION   DBMDYA010000534 DBMDYA010000000
VERSION     DBMDYA010000534.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10907_length_2110_cov_1.237929"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="ACZPFK_11580"
     CDS             <1..525
                     /locus_tag="ACZPFK_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406991.1"
                     /translation="CWRTVSFVRPQLSVVDYPDGYFAGRNEQIAQVERLKARLGEEER
                     VFYICTGDDGRRWFMSYYDFYPDVLLDYSLGGAGISPESRLNTVSIPRFFTREQAARF
                     LAEGVTLTPEVWCEYLEASGCTAVYLERVDESFQQVYGALFEGGAESGGLYRVQGDAQ
                     NMRFLPIPEEVSAR"
     gene            522..1184
                     /locus_tag="ACZPFK_11585"
     CDS             522..1184
                     /locus_tag="ACZPFK_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406992.1"
                     /translation="MNRIKKLGGALARLPGRDKRLVALCYLLFFGLSVLASLYSFAED
                     RVQRALGNVTTAGISAADFDWTDLEQGADGALLTTSADPRMVLRDSPAYVRGVRIEAV
                     FRNKDPGEFCIFYKPRPGMEDFDANYRVWAHVEEDGSYTFTLPRGKIYGLRLDPGIYA
                     GIEMEITSITLNPQRSFGSYFVPSRLWLLCQLVAPALIASAVRCAAEVFCRGPRKRGT
                     GT"
     gene            1181..>2110
                     /locus_tag="ACZPFK_11590"
     CDS             1181..>2110
                     /locus_tag="ACZPFK_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1406993.1"
                     /translation="MSSFFTLLSFFSLLIFAAWLHLYCSLPAALAPLVTACALMLWFS
                     FWGCLGLLAAAGWICFLLALAALVDLIRRSDSRFWKSFFCPGVLLFCLASLGVIFLFS
                     LRKPLFLEWDEFSFWGTAAKLVKQNDELYTTAKIGWAWTATQKPGLIVFSYFFQFFGV
                     FEEWRVMAALDVLQFAVFGALLAPLTRRQWACAVPATAAAFLVPAVLVLYRALDVPSN
                     LYMSAHSDIPMGVLFAGVFAVAYGCNGERKNLRWPTLVTLAALTLTRDTALPLALIAA
                     AAGAAAVEEHRQRRGHRPAHRPGKPDAGDAEQAR"
BASE COUNT          321 a          619 c          697 g          473 t
ORIGIN      
        1 tgctggcgca cggtcagctt cgtgcggccc cagctctcgg tggtggatta cccagacggc
       61 tactttgcgg gccgaaacga gcagatcgcc caggtggagc ggctcaaagc gcgcctggga
      121 gaagaggagc gggtttttta catctgcacg ggggacgatg gtcggcgctg gtttatgagc
      181 tattatgatt tttatcccga cgtgctgctg gactacagcc tgggcggcgc gggcatctcg
      241 cccgagagca ggctgaacac ggtctcgatc ccgcggtttt tcacccggga gcaggcggcg
      301 cgctttctcg ccgagggcgt gaccctgacg ccggaggtct ggtgcgaata tctcgaggcc
      361 agcggctgca ccgcggtgta tctggagcgg gtggacgaga gctttcagca ggtctacggc
      421 gcgctgtttg agggcggcgc cgagagcggc ggcctctacc gcgtccaggg ggacgcgcag
      481 aacatgcgct ttctccccat cccggaggag gtgagcgccc ggtgaaccgg atcaaaaaac
      541 tgggcggcgc cctggcccgc ctgccgggca gggacaaacg gctggtggcc ctgtgctatc
      601 tcttgttctt cggcctcagc gtgctggcct cgctttacag ctttgccgag gaccgggttc
      661 agcgggccct gggcaatgtg acgaccgccg ggatcagcgc cgccgatttt gactggaccg
      721 acctggagca gggggcggac ggcgcgctgc tgaccacctc cgccgacccg cgcatggtgc
      781 tgcgggactc gcccgcctat gtgcgcgggg tgcgcatcga agcggtcttc cgcaacaagg
      841 acccgggcga gttctgcatc ttttacaagc cgcgccccgg catggaggat ttcgacgcca
      901 actaccgggt ctgggcgcat gtggaggagg acggcagcta tactttcaca ctgccgcgcg
      961 gcaagatcta cggtctgcgg ctcgaccccg gcatctacgc cggcatcgag atggagataa
     1021 caagcatcac cctgaacccg cagcggagct ttgggtcgta ttttgtgccg tcccggctct
     1081 ggctgctctg ccaactggtc gccccggcgc tcatcgccag tgcggttcga tgcgcggccg
     1141 aggtcttttg caggggaccg aggaaaaggg ggacggggac atgagctcgt tttttacact
     1201 cctgagcttt ttctcactcc tgatttttgc agcgtggctg catctgtact gctctcttcc
     1261 ggctgcgctg gcacccttgg tgaccgcctg tgccctcatg ctctggtttt ctttttgggg
     1321 ctgtctcggc cttttggccg cagcggggtg gatttgcttt cttctcgcgc tggcggcgct
     1381 tgtggatttg attcggcgca gtgacagcag gttttggaaa agcttttttt gtcccggtgt
     1441 gttgctgttc tgcttggcca gcctgggtgt gattttcctt ttttctctgc gcaagcccct
     1501 ttttttggag tgggacgagt tcagtttttg gggaacagcc gcaaaactgg tcaaacagaa
     1561 tgatgagctg tacaccactg caaagatcgg atgggcgtgg actgcgacgc aaaagccggg
     1621 ccttatcgtc ttcagctatt tttttcagtt ttttggcgtt tttgaagagt ggcgcgtgat
     1681 ggcggccctg gatgtgctgc agttcgcagt gttcggcgcc ctgcttgccc cgctcacacg
     1741 gcgtcaatgg gcgtgcgctg tgccggccac ggccgctgct tttttggtgc ccgcggtgct
     1801 tgtactttac cgcgctttgg atgtgccgag caatctctat atgtccgccc attccgatat
     1861 ccccatgggg gttctctttg cgggagtgtt tgcggtggcg tatggctgca atggagaacg
     1921 gaaaaatctg cgctggccca ccctggtgac gttggcggcc cttactttga ccagggacac
     1981 ggctttgccg ttggcactga tcgcggcggc ggccggcgcc gcggcggtag aagaacaccg
     2041 acagcgccgt ggccaccgcc cagcccaccg gccaggcaaa ccagacgccg gcgacgccga
     2101 acaggcccgc
//
LOCUS       DBMDYA010000535         2109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10913_length_2109_cov_1.397908, whole genome shotgun sequence.
ACCESSION   DBMDYA010000535 DBMDYA010000000
VERSION     DBMDYA010000535.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10913_length_2109_cov_1.397908"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="ACZPFK_11595"
     CDS             <1..1174
                     /locus_tag="ACZPFK_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1406994.1"
                     /translation="LVTMVPRNILSAEVDAFLSRTSFLILGLVTAFTGVLIAAIRAQS
                     RYRARIEEMAFGDPVTGGESNICFLMEAEALLNRTPRQAFFLVSLNLRNFSMLNEQGG
                     RAHGDGVLKEVYQLLRQELVLPGERVARGEADTFYLLLAVVDRPLALRRLERMTVQLS
                     SLGGAAGPLRAKVGIYALPEGQIDLALAENRADLARKMGEGDGQPGCAFYTEEMGARL
                     REEHELLTELEPGLEHGEFLVYFQPKFSPQTEEIGGAEALVRWQHPTRGFISPGVFIP
                     LCESSDLICRVDLFVFEEVCRRLSQWAALGRPLIPISVNLSRRHLMDSDFLLDYKAIA
                     DRYQVPAGLIELELTESLMFSLAEFHQMRDLLNEIHRLGFSCSLDDFGFGFSSLGL"
     gene            1201..1440
                     /locus_tag="ACZPFK_11600"
     CDS             1201..1440
                     /locus_tag="ACZPFK_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1406995.1"
                     /translation="MDRSFFVHGQEEPRAQVVIRSMVQMAQKLGVSVVAEGVEQREQV
                     EFLREIGCDLIQGFYYSPPLSTEKFEALAFGAPLV"
     gene            complement(1585..>2109)
                     /locus_tag="ACZPFK_11605"
     CDS             complement(1585..>2109)
                     /locus_tag="ACZPFK_11605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1406996.1"
                     /translation="RRMFPLSRERDETFIGGLSMGGFGALRNGLCYADTFGGIAALSG
                     ALHMFEFEPGDPRREMLVREDACMGDWQQASASDKNPAVALRELSLRVKAGEAEFPRI
                     YMACGTEDGLLGANRSFRDKLLAAGAPLTYREGPGAHDWAFWDAQLPQVLDWLPLESA
                     AAGLNSGNVRPEKK"
BASE COUNT          344 a          673 c          651 g          441 t
ORIGIN      
        1 tcttgtgacg atggtgcctc gcaacatcct ctccgccgag gtagacgcgt tcctctcccg
       61 cacatccttt ttgatcctcg gtcttgtgac cgctttcacc ggcgtgctca tcgcggcgat
      121 ccgggcccag agccgttatc gcgcccgcat cgaggagatg gcctttggcg accctgtcac
      181 cggtggcgag agcaacatct gctttttaat ggaggcggaa gccttgctca accgcacgcc
      241 gcggcaggcg ttcttcctgg tttcgctgaa cctgcgcaac ttcagcatgc tcaacgagca
      301 gggcggccgc gcccatgggg acggcgtgct caaagaggtc taccagctgc tgcggcagga
      361 gctggtgctg cccggcgagc gggtggcccg cggggaggcg gatacctttt atctgctgct
      421 tgcggtcgtg gaccgcccgt tggcgcttcg ccgcttagag cgcatgaccg tgcagctctc
      481 ctctcttggg ggcgctgccg ggccgctgcg ggccaaggtg gggatctacg cgctccccga
      541 ggggcagatt gacctggcgc tggcggaaaa ccgagccgat ctcgcccgca agatggggga
      601 gggagacgga cagcccggct gcgcttttta taccgaagag atgggcgccc gcctgcggga
      661 ggagcacgaa ttgttgacgg agttggaacc gggcctggag cacggcgagt ttctggtgta
      721 ttttcagccc aaattttctc cccaaacaga ggagatcggc ggcgcggaag ccctggtgcg
      781 ctggcagcat ccgacccggg gcttcatcag cccaggggtt ttcatcccct tgtgtgagag
      841 cagcgacctg atctgccgtg tggacctctt tgtctttgaa gaggtctgcc gccgcctttc
      901 ccagtgggcg gccctgggcc ggcccctgat ccccatctcg gtgaatctgt cgcgccgaca
      961 cctcatggac tccgatttcc tgttggatta caaggctatc gccgaccgct atcaggtgcc
     1021 ggcgggcctg atcgagctgg aactgaccga gtcgttgatg ttttcattag cggaatttca
     1081 tcagatgcgc gacctgctga acgagatcca tcggctgggc ttctcctgct cgctggacga
     1141 tttcggtttt ggtttttctt ccctggggct ctgaaagagc tgcagatcga ctgcctcaag
     1201 ctggatcgca gcttttttgt ccatggccag gaggagccgc gcgctcaggt tgtgatccgt
     1261 tcgatggtgc aaatggccca aaagctgggc gtctccgtcg tggccgaggg ggtggagcag
     1321 cgcgagcagg tggaatttct gcgggagatc ggctgcgacc tcatccaggg cttttattac
     1381 tcgcctcctc tctcgacaga aaaatttgag gcgcttgcct ttggggcgcc gcttgtttag
     1441 caaagcgcgc cgcctttttt aaaagcagaa gacagtcccc cagcctctgt aacctgaaag
     1501 cgggcacaaa aatacaattt gtatttttgt gcccgcttat tcttttcctt ttgcctctgc
     1561 ggcccgctta ccggccgttt tcgcctattt tttctccggg cgcacgttgc cgctgttcag
     1621 gccggcggcc gcactctcca gaggcagcca gtccagcacc tgcggcagct gcgcgtccca
     1681 aaacgcccag tcatgcgcgc ccggcccctc ccggtaggtc agcggcgcgc ccgccgccag
     1741 cagcttgtcg cgaaacgagc ggttcgcccc caacagcccg tcctcggtgc cgcaggccat
     1801 atagatgcgc gggaactccg cctccccggc cttgacgcgc agggacagct cccgcagcgc
     1861 cacggccggg tttttgtcgc tggccgacgc ctgctgccaa tcgcccatac aggcgtcctc
     1921 gcggaccagc atctcccggc gcgggtcgcc gggttcaaat tcaaacatgt gcagcgcgcc
     1981 ggacagcgcc gcaataccgc caaaggtatc ggcgtagcac aagccgttgc gcagcgcgcc
     2041 gaacccgccc atggagaggc cgccgatgaa agtctcgtcc cgttcccgcg agagcggaaa
     2101 catcctgcg
//
LOCUS       DBMDYA010000536         2108 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10921_length_2108_cov_1.778774, whole genome shotgun sequence.
ACCESSION   DBMDYA010000536 DBMDYA010000000
VERSION     DBMDYA010000536.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10921_length_2108_cov_1.778774"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /gene="cas8c"
                     /locus_tag="ACZPFK_11610"
     CDS             complement(<1..1638)
                     /gene="cas8c"
                     /locus_tag="ACZPFK_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HIR1406997.1"
                     /translation="MILQALANYYQVLAARKEIAPPGRASVGVSFALELAPDGALLRV
                     LPLKVQPAEGKKELPQSMWVPEQVKRSSGVAANFLCDNSSYLLGIDEKGKPGRTLQCF
                     AAAGELHRAVLDGVDSPAARAILNYFDSWDPGAALSHPAVAPLADQLLAGANLIFSVN
                     DKWAQEDEKISAAWDAFKTGHSGEKEMVCLVTGERAPAARLHAAIKKVHDAQPTGASL
                     VSFNAPSLESFGKEMADDTGQGWNAPVSESAAFAYTTALNHLVADHNHVQRIGDTTVV
                     FWAEDGEPAYQDVFGSSVFGAPNPKMTETDLKSIFKSLAHGMPVDFEGIPLNPENHFY
                     VLGLAPNVARLSVRFFFADTFRRLLRHVEEHYQRLEIVKPAYEKFETLPLWRLLNETV
                     NQNATKKDASPPMAAAVARAVLTGGDYPASLFEAVMLRIRAEREITYGRAAILKAYFI
                     KNRSVSLPEEVTQVRLKEECNYVPYLLGRLFSVYEAIQQEANPGINATIKDKYFNSAA
                     AMPATVFPVLIGLADKHLKKLKKEERIYNHYNKMLTGL"
     gene            complement(1635..>2108)
                     /locus_tag="ACZPFK_11615"
     CDS             complement(1635..>2108)
                     /locus_tag="ACZPFK_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HIR1406998.1"
                     /translation="LTSIRRNEVKSKASAKEALSAMRGADRQLGIASSQDILQRASLV
                     LTDVHYVLDAHFEMTERAAPSDNPGKFQDILTRRLEKGQCYHMPCFGCREFPAYFRAW
                     RGGPIPVPRECRDRRDLGLMLYDLDYSNPRDIQPMFFRAVLENGTVQVAGEEVYR"
BASE COUNT          388 a          671 c          631 g          418 t
ORIGIN      
        1 gagcccggtg agcatcttat tgtaatggtt atagatcctc tcttcttttt tcagcttttt
       61 gaggtgcttg tccgccaggc cgatcaacac ggggaatacc gtggcgggca tcgccgccgc
      121 gctgttgaaa tatttatcct tgatggtggc gttgatgccg gggttcgcct cctgctggat
      181 ggcctcgtac accgaaaaca gccgtcccag cagatagggt acatagttgc actcctcttt
      241 taatctcacc tgtgtgacct cctctggcag cgaaacgctg cggtttttta taaaataggc
      301 tttcaggatc gccgcccggc cgtaggtgat ctcccgctcc gcccggatgc ggagcatgac
      361 ggcttcgaac agcgaggcgg gataatcccc gccggtcagc accgcccggg cgacggccgc
      421 ggccatgggc ggcgaggcgt ctttctttgt ggcgttctgg ttcaccgtct cattcaacag
      481 ccgccagagc ggcagggtct cgaatttttc ataggccggc ttgacgatct ccagccgctg
      541 atagtgctcc tccacatgcc ggagcaggcg ccggaacgta tcggcgaaaa agaaccgcac
      601 cgacaggcgc gccacattgg gggcaagccc cagcacatag aaatgattct ccggattgag
      661 cgggatgccc tcaaaatcca ccggcatgcc atgggccaag cttttgaaga tgctcttcag
      721 gtctgtctcc gtcattttgg ggttcggcgc gccgaaaaca gaagagccga aaacatcctg
      781 ataggcaggc tcgccatcct ccgcccaaaa gacgacggtg gtgtccccga tccgctggac
      841 gtggttatgg tcggccacca aatggttcag cgccgtggtg taggcaaacg cggcgctctc
      901 gctgaccggg gcgttccagc cctggccggt atcgtccgcc atctctttgc cgaaagattc
      961 cagcgagggg gcgttgaacg agaccaaaga ggccccggtg ggctgcgcat catgcacctt
     1021 tttgatggcg gcatgcagcc gcgcggcggg cgcccgctcg cccgtgacca ggcagaccat
     1081 ctctttctcc ccgctatggc ccgtcttgaa cgcgtcccag gcggcgctga ttttttcatc
     1141 ttcctgggcc catttgtcgt ttaccgaaaa gatcaggttt gccccggcca aaagctggtc
     1201 cgccaaaggc gctaccgcgg ggtggctcaa ggccgcgccc gggtcccagc tgtcgaaata
     1261 attgaggatc gcccgggcgg cagggctgtc caccccatcc agaacggccc ggtgaagctc
     1321 acctgccgcc gcaaagcatt gcagcgtgcg gcccggctta cccttttcgt cgatccccag
     1381 cagataggag ctgttatcgc acaaaaaatt ggccgccacg ccggaggacc gcttcacctg
     1441 ctcggggacc cacatgctct gcggcagctc ttttttcccc tcagcgggtt ggactttcag
     1501 cggcaggaca cgcagcaggg cgccgtcggg ggcaagctcc agtgcaaacg acacgcccac
     1561 gctcgccctg ccggggggag cgatctcctt acgcgctgcg agcacctgat aataattcgc
     1621 caaagcctgc aggatcatcg gtacacctcc tcgcccgcga cctggaccgt gccgttctcc
     1681 aaaaccgcgc ggaaaaacat gggctggatg tccctggggt tagagtaatc caggtcgtag
     1741 agcatcaggc ccaggtcgcg cctgtcccgg cactctctcg gcacagggat ggggccgccc
     1801 cgccaggcgc ggaaatatgc cggaaactcc cggcagccga aacagggcat gtgatagcac
     1861 tgcccttttt ccagccggcg ggtcaggata tcctggaact tgcccgggtt gtcggagggg
     1921 gcggcccgct cggtcatctc aaaatgcgcg tccagcacat agtgcacatc cgtcagcacc
     1981 agcgacgcgc gctggaggat atcctgcgag gacgcgatgc ccagctgcct gtccgcccct
     2041 cgcatcgccg agagggcttc cttggcggac gccttgctct tgacctcgtt gcggcggatg
     2101 ctggtgag
//
LOCUS       DBMDYA010000537         2108 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10922_length_2108_cov_1.655705, whole genome shotgun sequence.
ACCESSION   DBMDYA010000537 DBMDYA010000000
VERSION     DBMDYA010000537.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10922_length_2108_cov_1.655705"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="ACZPFK_11620"
     CDS             <1..1756
                     /locus_tag="ACZPFK_11620"
                     /inference="COORDINATES: protein motif:HMM:NF022812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HIR1406999.1"
                     /translation="GTAMRDESLAQAEALKGTEWENAALRNAAFFSVGLALQQPETEA
                     PAAVRDVVAQELELIEKAEGVGASPVMNLGGDAAEKMEMEDYSQYIPRGYYTTEEALE
                     RYFKAMMWYGRLTFRQSDEDQSRSALLMTLALTGGEALETWEKIYTVTSFFVGDSDDA
                     GIYEYAPLIEKAYGGRPVVKELPQKQTEWTAFLQALKELKPPAINSIPIFDEGIQPDK
                     EEAVAGFRFLGQRFTLDASIFQQLVYRQVGANNADERRMLPSALDIPAALGSAEAEKL
                     LTEAGAMEYQGYGENLAQLQSYLAQPPQQLWTGSLYGGWLNTLRPLLAEKEEGWPQFM
                     QNEAWTRKNLNSFLGSWTELKHDTVLYAKQVYAEMGGAGMEERDDRGYVEPEPVLYSR
                     LAGLSTATAEGLRHYGVLRDADAENLARMAELARQLMVISEKELRDELLSDEEYDLIR
                     GFGGQLEHFWYDALADESGDGATITSLDHPAAIVTDVATDPDGGRVLEVGAGKVNEIF
                     VIVSVDGVLRLARGGVFSFYEFAQPLDQRLTDEEWRKMLGIYRFDENGNFDDQSVEVP
                     RPGWTSSFFTPAYYSWQQ"
     gene            1753..>2108
                     /locus_tag="ACZPFK_11625"
     CDS             1753..>2108
                     /locus_tag="ACZPFK_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407000.1"
                     /translation="MMKKAGRAGRLRRWGVLCLVLAALCGALAVGLLWQDGAFLARWQ
                     QKTEPWDLDGDGRTETLFLERRSFSVLSGGQTLWKTDKDWKVEDYLCGDIDGDGAEEL
                     LLLVWKRGSYGPRRPF"
BASE COUNT          396 a          616 c          741 g          355 t
ORIGIN      
        1 aggcaccgcg atgcgggacg agagcctggc ccaggccgaa gcgctcaagg gcaccgagtg
       61 ggaaaatgcg gccctgcgca acgcggcgtt cttttccgtc gggctggcgc tgcagcagcc
      121 ggaaacggag gccccggccg cggtgcggga cgttgtcgcg caggagctgg agcttatcga
      181 gaaagcggag ggcgtcggcg cctccccggt gatgaacctg ggcggcgacg cggcggaaaa
      241 gatggagatg gaggactatt cccagtatat cccccgcggc tattatacca ccgaggaggc
      301 gctggagcgc tatttcaagg ccatgatgtg gtatgggcgg ttgacctttc ggcagagtga
      361 tgaagaccag agccgcagcg cgctgctgat gacgctggcg ttgaccggcg gcgaggcgct
      421 ggagacctgg gaaaagatct ataccgtcac cagctttttt gtgggcgaca gcgacgacgc
      481 cggtatctat gagtacgcgc cgctgatcga aaaggcctac ggcggccgtc cggtggtaaa
      541 agagctgccg caaaaacaga cggaatggac ggcatttttg caggcgctca aagagctgaa
      601 accgcccgcc atcaactcca tccccatctt tgacgagggc atccagccgg acaaggagga
      661 ggccgtcgcg ggcttccgct ttttggggca gcggttcacg ctggacgcct ctatttttca
      721 gcagctggtt taccgccagg tgggggccaa caacgccgat gagcggcgca tgctgccctc
      781 ggcgctggac atcccggcag cgctgggctc cgcggaggcc gaaaagctcc tgaccgaggc
      841 cggggcaatg gagtaccagg gctacggtga aaacctggcc cagcttcaaa gctacctcgc
      901 acagcccccg cagcagctct ggaccggcag cctttacggc ggctggttga acaccctgcg
      961 ccccctgctc gccgaaaagg aggagggctg gccccagttc atgcagaacg aggcgtggac
     1021 gcgcaaaaac ctgaacagct ttctcggcag ctggacggag ctcaagcatg acaccgtcct
     1081 ctatgcaaaa caggtctacg ccgagatggg cggcgccggc atggaggagc gggacgaccg
     1141 cggctatgtg gagccggagc cggtgctcta ctcccgtctg gcgggccttt ccaccgccac
     1201 tgcggagggg ctgcggcact acggcgtgct gcgggacgcg gacgccgaga atctggcccg
     1261 catggcggag cttgcccggc agctgatggt catctcggaa aaagagctgc gggacgagct
     1321 gctgagcgac gaggagtacg accttatccg cggcttcggc ggtcagctgg agcatttctg
     1381 gtacgacgcg ctggcggatg agagcggcga cggcgccacc atcaccagtc tggaccaccc
     1441 ggcggcgatc gtcaccgacg tggccaccga cccggacggc ggccgggtgc tggaggtggg
     1501 cgccggcaag gtgaacgaaa ttttcgtcat cgtcagcgtg gacggcgttt tgcggctggc
     1561 gcgcggcggc gtgttctctt tttacgagtt tgcccagccc ctggaccagc gcctcaccga
     1621 cgaggagtgg cgcaagatgc tgggcatcta ccggtttgac gaaaacggca attttgacga
     1681 tcagagcgtc gaggtgcccc ggccgggttg gacctcttct tttttcactc cggcctacta
     1741 tagctggcag caatgatgaa aaaagcaggc agagcgggcc gcctgcgccg gtggggcgtg
     1801 ctctgccttg tgctggctgc tctctgcggc gcgctggccg tggggctgct ctggcaggac
     1861 ggggctttcc tcgcccgctg gcagcaaaaa acagagcctt gggacctgga cggtgacggc
     1921 cggacagaga cgcttttttt ggagcggcgc tctttctcgg tgctgagcgg cgggcaaacg
     1981 ctctggaaaa cggacaaaga ctggaaagta gaggactatc tgtgcggcga tatcgacggc
     2041 gacggcgcgg aggaactgct gctgctggtg tggaagcggg ggagctacgg accgcggcgg
     2101 cctttctg
//
LOCUS       DBMDYA010000538         2107 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10932_length_2107_cov_1.442173, whole genome shotgun sequence.
ACCESSION   DBMDYA010000538 DBMDYA010000000
VERSION     DBMDYA010000538.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10932_length_2107_cov_1.442173"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="ACZPFK_11630"
     CDS             <1..946
                     /locus_tag="ACZPFK_11630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1407001.1"
                     /translation="TTARDMALITRAALQNSEFVRVFSTAEYVIPATNKQAEARNIGA
                     SNKMIFDFTKYYYPGIIGGKSGYTTEAGHTLVSAAERDGRTLISVVLAEPKNAAIYED
                     SVALLDYGFDAFTSVTLTPQELGAEIEAADATLATEEPVTLLLAAGTGVESLQKSYQV
                     EKSDRYGMTVRVGLSLPEGDAAQYGEVASAELRYVVPLAAASAGAGAAPEKGTDWKAV
                     GLTALRWLGVAAAAALAVFVCLLLYVRLRYDIRRLLRRYRRRRGAGRAQSMPQHRAAP
                     APRQMARRPAPPARPRAVGEGRSLPPAQRPLRGSTRYD"
     gene            1249..>2107
                     /locus_tag="ACZPFK_11635"
     CDS             1249..>2107
                     /locus_tag="ACZPFK_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1407002.1"
                     /translation="MERIVYRNANIVGYGKGYSLELVDGRVTGITPTDKMPAEGVDLK
                     GRLIAPRFVDSHMHLDKAMVPAAAGAKGLLAAIAASAEYLQQVPQDCLLEDVTRRSGQ
                     VVDMALAAGTGCIKTNLLIGGAMGWASLLSLQELRHRYRGGMRLLGAVPLEEGEERAF
                     ERHAAAGEIDFIAGYPSLTKDYRAEVDRIFRMAEKYGLPVDLHVDESDVPDVSCFCYV
                     LQKTIEMGMQGRVTCGHVTALSAPGLPQREIDRAVGLAVRAGVCVTTLTSCNLFLMSS
                     SRRGPTLVRE"
BASE COUNT          350 a          649 c          772 g          336 t
ORIGIN      
        1 caccaccgcg cgggatatgg ccctcatcac ccgcgccgcg ctgcagaaca gcgaatttgt
       61 gcgggtcttt tccaccgccg aatacgtgat accggccacc aacaagcagg cggaggcccg
      121 caacatcggc gccagcaata agatgatctt tgatttcacc aaatattact atcccggcat
      181 catcggcggc aagagcggct ataccaccga ggcggggcac acgctggtct cggccgcaga
      241 gcgggacggg cgcaccctca tcagcgtggt gctggccgag cccaaaaacg ccgccatata
      301 tgaggacagc gtggcgctgt tggattacgg ctttgacgcc ttcaccagcg tgactttgac
      361 cccgcaggag ctgggggcgg agatcgaagc ggcggacgcg accctggcca ccgaggagcc
      421 ggtcactctg ttgctggccg cgggcaccgg ggtggagagc ctgcaaaaga gctatcaggt
      481 ggaaaagagc gaccgctacg gcatgacggt gcgggtgggc ctctcgctgc cggagggcga
      541 cgccgcgcaa tacggcgagg tggcgagcgc cgagctgcgg tacgtggtgc cgctggccgc
      601 cgcgtcggcg ggcgcggggg cggcccccga aaaagggacg gattggaaag cggtcggcct
      661 cacggcgctc aggtggctgg gcgttgcggc ggccgccgcg ctggcggtct ttgtgtgcct
      721 gctgctctat gtgcggctgc ggtacgacat ccgccgcctg ctgcgccgct atcgccgccg
      781 caggggcgcc gggcgcgcgc agagcatgcc ccagcacaga gcggcgcccg ccccccggca
      841 gatggcacgc agacccgcgc cccccgcgcg cccgcgggcg gtgggggagg gccgcagcct
      901 gccaccggcc cagcgcccgc tgcggggcag cacgcgctac gactgagaca cgatcctttt
      961 ccgctgtgta ttgccgggtg aaaacgtcag acggtccggc aataaaatga taggtcctga
     1021 gcggcgcggc agcagctgtg tcccgggctc cgcatcaggc cccttgtcgg caaaggggcc
     1081 tggggaggcg cgcttcagcc cccccttgag ggcggctggg ccgcttttgg ggcggggccg
     1141 agtgggacgg ggcggggacg gcttttttgt agggcgggag cgcccgcttt gcaaaagccg
     1201 gctcctaccg ctgtgtgcgg ggcgtctggg gccaaaggag cggacgatat ggaacggatc
     1261 gtttatcgca acgccaacat cgtgggctat ggaaagggct attccctgga gctggtggat
     1321 ggcagagtca ccggcatcac gcctacggac aagatgccgg cagagggcgt ggatctgaaa
     1381 gggcggctga tcgcgccccg ctttgtggac agccacatgc atctggacaa ggcgatggtc
     1441 cccgctgcag cgggggcaaa gggattgctg gccgcaatcg cggcttccgc ggagtatctg
     1501 cagcaggtgc cacaggactg cctgctggag gacgtcaccc gccgcagcgg gcaggtggtc
     1561 gacatggcgc tcgccgccgg cacgggctgt atcaagacga acctgttgat cggcggtgcg
     1621 atgggctggg cctccctgct ctctttgcag gagctgaggc acagataccg gggcgggatg
     1681 cggctgttgg gcgccgtccc gctggaggag ggggaggagc gggcctttga gcggcacgcc
     1741 gccgcgggcg agatcgattt tatcgcggga taccccagcc tgacaaagga ttatcgggcc
     1801 gaggtggacc gcatcttccg catggcggaa aagtacggtt tgccggtgga cctccatgtg
     1861 gatgagtcgg acgtgccgga tgtcagctgc ttttgctatg tgctgcaaaa aaccattgag
     1921 atggggatgc aggggcgggt cacctgcggc cacgtcaccg cgctttccgc cccgggtctg
     1981 ccgcaaaggg agattgaccg ggccgtcggc ctggcggtgc gcgccggggt ctgcgtgacc
     2041 accctgacct cctgcaacct gtttttgatg agcagttccc ggcggggccc caccctggtg
     2101 cgggagc
//
LOCUS       DBMDYA010000539         2102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10970_length_2102_cov_1.297851, whole genome shotgun sequence.
ACCESSION   DBMDYA010000539 DBMDYA010000000
VERSION     DBMDYA010000539.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_2102_cov_1.297851"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(30..446)
                     /locus_tag="ACZPFK_11640"
     CDS             complement(30..446)
                     /locus_tag="ACZPFK_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407003.1"
                     /translation="MAEHLWFWLIDLIIPGLLLATGAAWRRFAPRRIDPHRGYRTARS
                     TASGVAWRYAQRYFSALCLRFGATLLLLVSADRLINLLAGLMPPARLAYLNTAVGLGL
                     FLSIVPLVELALAGRFDGRVDGGDTRAGARPLRPAA"
     gene            778..1617
                     /locus_tag="ACZPFK_11645"
     CDS             778..1617
                     /locus_tag="ACZPFK_11645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1407004.1"
                     /translation="MAKIRIICVDLDGTLLRNDHSTIPPENLEAIREAATAGVAVVPA
                     TGRILGRVPRAVRQIPEIRYAIVINGAEVVDLADGGLLHSAYLPVQTALRIVARGRRL
                     GLTPEVYQHDHMLIQRDDLQLLQTRPIERDHLNYLLRHETPLEDIEAHLRSHPEGICK
                     VNLPYFLDEGLRWRLRAELEAAEEVEAATSMEKNLELNARGASKGAAVRALCRALGVS
                     PQEVMAIGDGDNDRELLAAAGCPVAMGNATPQLRALAGRVTGTNEEGGVAMAIRAALK
                     GEL"
     gene            1621..>2102
                     /locus_tag="ACZPFK_11650"
     CDS             1621..>2102
                     /locus_tag="ACZPFK_11650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1407005.1"
                     /translation="MGYLALLEQFEPCCEQEEGDKRVVLDYCRTFPQNILTRENEFAH
                     LTSSGLVLNPSLTRVLMVYHNIYHSWSWTGGHCDGDPDLLAVALREVQEETGLAQVRP
                     LTGELMGLDILPVPGHVKRGRYVPAHMHLSPAFVLIAPEGQAVREKPDENSGVRWFDA
                     "
BASE COUNT          355 a          675 c          741 g          331 t
ORIGIN      
        1 actgcggcgg ccaggctgcg ccgggccggt caggccgcgg ggcgcagggg gcgggccccg
       61 gcgcgggtgt cgccgccgtc cacccgtccg tcgaagcgcc cggcgagcgc cagctccacc
      121 agcggcacga tggaaaggaa cagccccagg ccgacggcgg tgttaagata ggccagccgc
      181 gcaggaggca tcagcccggc cagcaggttg atgagccggt ccgccgacac aagcagcaac
      241 agcgtggcgc caaagcgcag gcaaagggcg ctgaaatagc gctgcgcata gcgccaggcc
      301 acgccgctgg cagtgctgcg cgcggtgcgg tagccccgat gcgggtcgat gcggcgcggg
      361 gcgaagcgcc gccacgccgc gccggtggcc aacaaaagcc ccggtatgat caggtcaatc
      421 agccaaaacc agagatgctc cgccatggtg ttctccgccc tttcctgcca gaatttgtcg
      481 ttctattcta agaaacgagc ttccggcccg gtttattaca attgcatggg gccattttaa
      541 cagatgattt cttatctttt ccttaagatc ggcggcgtcg cgcgatcttt cacactcttt
      601 tcaccgcggc tttttgtctg ccctgttcct gcggcccctg tccgcctccg ccggggcaaa
      661 gcgccgccgc ggctcttttt ttttgcctcc gcttcgatta taatggtagg gattggaaga
      721 tacatgcccc ggcccggccg ggccgccccc cggggcgcgg ggcgaaaggg gagccacatg
      781 gcaaagatca ggatcatctg cgtcgatctg gacggcacgc tgctgcggaa cgaccacagc
      841 accatcccgc cggaaaatct ggaggccatc cgggaggccg ccacggcggg ggtggcggtg
      901 gtgcccgcca ccggccgtat tttggggcgg gtgccgcgcg cggtgcggca gatccccgag
      961 atccgctacg ccatcgtcat caacggggcc gaggtggtgg acctcgccga cggaggcctg
     1021 ctgcacagcg cctacctgcc ggtgcagacc gcgctgcgca tcgtggcgcg cgggcggcgg
     1081 ctgggcctca cgcccgaggt gtaccagcac gatcacatgc tgatccagcg ggacgatctg
     1141 cagctgctgc agacgcggcc catcgagcgg gaccacctga attatctgct gcggcacgag
     1201 acgcctctgg aggatatcga ggcccatctg cgcagccacc cggagggcat ctgcaaggtg
     1261 aacctgccct attttctcga cgaggggctg cggtggcggc tgcgggcgga gctggaggcc
     1321 gccgaggagg tggaggccgc cacctcgatg gaaaaaaatc tggagttgaa cgcgcgcggc
     1381 gcctcgaaag gggcggcggt acgggcgctc tgccgcgcgc tgggggtctc gccgcaggag
     1441 gtgatggcca tcggggacgg cgacaacgac cgcgagctgc tggcggcggc cggctgcccg
     1501 gtggccatgg gcaacgccac cccgcagctg cgcgcactgg cgggccgggt caccggcacc
     1561 aacgaggagg gcggggtcgc aatggcgatc cgcgccgcat tgaaagggga gctttgagag
     1621 atgggatatc tggcgctttt ggaacagttc gagccctgct gcgagcagga agagggggac
     1681 aagcgggtcg ttttggacta ttgccgcacc tttccgcaga atatcctcac ccgtgaaaac
     1741 gagttcgccc atctcacctc cagcgggctg gtgctgaacc cgtccctgac gcgggtgctg
     1801 atggtctacc acaatatcta ccacagctgg agctggaccg gcggccactg cgacggcgac
     1861 ccggatcttc tcgccgtggc cctgcgcgag gtgcaggagg agaccggcct ggcccaggtg
     1921 cggccgctca ccggcgagct gatgggcctg gatatcctgc cggtgcccgg ccatgtgaag
     1981 cggggccgct acgtgcccgc ccacatgcat ctgtcgccgg ctttcgtgct gatcgcgccg
     2041 gaggggcagg cggtgcggga aaagccggat gaaaacagcg gcgtgcgctg gttcgacgcg
     2101 ga
//
LOCUS       DBMDYA010000540         2099 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_10985_length_2099_cov_1.500501, whole genome shotgun sequence.
ACCESSION   DBMDYA010000540 DBMDYA010000000
VERSION     DBMDYA010000540.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10985_length_2099_cov_1.500501"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(307..1188)
                     /locus_tag="ACZPFK_11655"
     CDS             complement(307..1188)
                     /locus_tag="ACZPFK_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407006.1"
                     /translation="MREQIEAVQRMQDYIEAHLGEEITPADLARAALFSPWHACRLFT
                     AWLGLTPADYIRRLRLSRSALRLRDESCRVTDVAFEAGFGSVDGYQRAFYRAFGCNPR
                     QYAAHPVPLYLFNPYGVKYREMERKPMMESVKNVFVRLVEKPARNVVIKRGGHAADYF
                     AYCEEVGCDVWGLLRSIRGLGGEPVCLWLPDAYIAPGTSRYVQGVEVAEGEEVQAPEG
                     FDVIRLPAAEYLMFQGEPFAEEDYCQAIEEVQAAMKKFDPSGMGYVWDESNPRIQLEP
                     FGARGYIELAPVCKKRP"
     gene            complement(1456..1944)
                     /gene="ybaK"
                     /locus_tag="ACZPFK_11660"
     CDS             complement(1456..1944)
                     /gene="ybaK"
                     /locus_tag="ACZPFK_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1407007.1"
                     /translation="MAKKEEKTNVMRLLDREKIAYLPHAYDHADGVIDGAGVARKLGQ
                     DPARVFKTLVTRGASGNYAVFCLPVEKELDLKKAARAAGEKSVSMIHVAEINKVTGYI
                     RGGCSPLGMKKRYTTVFDASVRTLETVMVSAGRVGFQIELAPADLLQLADARTADLVT
                     DG"
     gene            complement(1914..>2099)
                     /locus_tag="ACZPFK_11665"
     CDS             complement(1914..>2099)
                     /locus_tag="ACZPFK_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407008.1"
                     /translation="HNKNHGKIAARRQESPRGRAGARCRAARFQKTPHPSAQLPGDAG
                     ECRRGIMKWQKKRKRPM"
BASE COUNT          353 a          741 c          615 g          390 t
ORIGIN      
        1 ccgcgctgcg cgcggagcag gcggcccccc ggccgccgca gggggcctaa gcgggcgcgg
       61 cggggcggca gcgcccggcc ggtattttga aaagagcgcg ccggccaggg gccgaggccc
      121 aacaacgccc tgaacacccg ttctcccgcc cgccgcgaac tatgtgcgcg gcggatataa
      181 aagaataaaa accggggccg ccgatccaaa gggatgaggc ggccccggtt ttttgctttg
      241 tctttcttgc tggccctgcc ggggcggcct gccgccgccg tggccggagc ttggttcggc
      301 cgggcgctac ggccgttttt tgcacaccgg cgcgagctcg atatagccgc gggcgccgaa
      361 aggctccagc tggatgcgcg ggttcgactc atcccagaca taccccatcc ccgacgggtc
      421 gaattttttc atcgccgcct gcacctcttc gatcgcctgg caatagtcct cctccgcaaa
      481 gggctcgccc tggaacatca ggtactcggc ggcgggcagg cgtatgacgt caaagccctc
      541 gggcgcctgg acctcctctc cctccgccac ctcgacgccc tgcacatagc gggaggtgcc
      601 gggcgcgata taagcgtcgg gcagccagag acagaccggc tcgcccccca ggccgcggat
      661 gctgcgcaaa aggccccata cgtcgcagcc cacctcttca caataggcaa aataatcggc
      721 ggcgtgcccc cccctcttga tgacgacgtt ccgcgccggt ttttccacga gccggacaaa
      781 gacgtttttg acagattcca tcatcggttt tctctccatt tctctgtatt ttacgccgta
      841 ggggttgaag aggtagagcg gcaccggatg agcggcatac tgtcgcggat tgcagccgaa
      901 cgcgcggtaa aacgcccgct gatagccgtc cacgctgccg aaaccggcct caaaggccac
      961 atccgtcacc cggcagctct cgtcccgcag ccgcagggcc gagcgcgaca gccgcaggcg
     1021 gcggatataa tccgcagggg tcaaccccag ccaggccgtg aacagccggc aggcgtgcca
     1081 gggtgaaaac agcgccgctc tggccagatc ggcgggggtg atctcctccc ccaggtgcgc
     1141 ctcgatatag tcctgcatcc gctgtaccgc ctcgatctgt tcccgcatcc tcttcacctc
     1201 cccgacatct tcatcataga caaaacggaa aaatatttct cgactatttt tgccccgctg
     1261 ccccccgccc aatcaaagcc cgcgtcagct gcgggcgatg caaaccgaaa cggtactctt
     1321 gccaattgcc gggccgccgc gccgccgcgc gcagcgggca gcgcaaaaac aatccccctg
     1381 cgcgtgggaa acgctgtttt tcccacaccc agagggatgg cttctctgtg ttttcggacc
     1441 cgccccgcgt cctgctcagc cgtccgtcac caggtcggcc gtgcgcgcat cggccagctg
     1501 cagcaggtcc gccggcgcga gctcgatctg aaagccgacc cgccccgccg agaccatcac
     1561 ggtctccagc gtccgcacgc tggcgtcgaa cactgtggta tatcgctttt tcatgcccag
     1621 cgggctgcag ccgccgcgga tatagccggt gaccttgttg atctccgcca catggatcat
     1681 cgacaccgat ttttccccgg ccgcgcgggc cgcctttttc aggtccagct ccttttccac
     1741 cggcaggcag aagacggcgt aattgcccga ggcgccccgg gtgaccaggg tcttgaaaac
     1801 gcgggcgggg tcctgtccca gcttgcgggc cacccccgcc ccgtcgatca ccccgtccgc
     1861 gtggtcgtag gcatggggca gataggcgat tttctctctg tccagcaggc gcatcacatt
     1921 ggtcttttcc tctttttttg ccatttcata atccccctcc ggcattctcc cgcatcgccc
     1981 ggcaactgag ccgatgggtg aggtgttttc tggaagcgcg ccgcccggca gcgtgcccca
     2041 gcgcggccac gcgggctttc ctgccgccgt gcggcgattt ttccatggtt tttattatg
//
LOCUS       DBMDYA010000541         2096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11007_length_2096_cov_1.419048, whole genome shotgun sequence.
ACCESSION   DBMDYA010000541 DBMDYA010000000
VERSION     DBMDYA010000541.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11007_length_2096_cov_1.419048"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..593
                     /locus_tag="ACZPFK_11670"
     CDS             60..593
                     /locus_tag="ACZPFK_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407009.1"
                     /translation="MTGQKQKRPGGTGLGVGASSILVIFVLLCLVTFAALLLVSARAD
                     WNLSRKAADHTLEYHAASNAAEETLAGADALLADCWRLAKGDESDYLARARAALAGLG
                     EVAEDGDGLRFSFSVQVNEGQSLACELRVRPPRPEKGTFYEIVRWQVESAGEWQPDEG
                     LPVYGGDGAAGALPGRP"
     gene            1027..2088
                     /locus_tag="ACZPFK_11675"
     CDS             1027..2088
                     /locus_tag="ACZPFK_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR1407010.1"
                     /translation="MDITVQLKDAVARGASDIFIVAGRPLVYKLNGQLTNVDEARLLP
                     PDTEEFVRQIYKLAKDRPMDRLMETGDDDFSFAVQGISRFRVSAYKQRSSLAAVVRVI
                     TFELPDPAALHIPEQVLRLADRTKGMVLVTGSAGSGKSTTLACMIDRINCTRADHIIT
                     LEDPLEYLHSHKKSIVSQRETGSDTESYLAALRGALRQSPDVILLGEMRDYETITVAM
                     TAAETGHLLFSSLHTVGAANTIDRILDVFPPTQQHQIAVQLAMVLQAVVSQQLLPTVD
                     GGLLPAFEIMNVNPAIRNMIRENKIHQIDTVIGSSADEDMISMDASILNLYRAGRISE
                     RTALTYAANPEILAKKLKL"
BASE COUNT          357 a          693 c          716 g          330 t
ORIGIN      
        1 cacggcgggc tgagacgcgg gcagaccgcg atcatcgaca gggaaaggag gctgcggcca
       61 tgaccggaca aaagcagaag cgcccggggg gcaccgggct gggggtgggc gcgtcctcca
      121 tcctggtgat ctttgtgctg ctctgtctcg tgacctttgc ggcgctgttg ctggtgagcg
      181 cccgggcgga ctggaacctc tcccgcaagg ccgccgacca cacgctggaa taccacgccg
      241 cctccaacgc ggccgaggag acgctggccg gggccgacgc cctgctggcg gactgctggc
      301 ggctggcaaa gggggacgag agcgattatc tggcgcgcgc ccgcgccgcc ctggccgggc
      361 tgggcgaggt ggcggaggac ggcgacgggc tgcgcttctc cttttccgtg caggtgaacg
      421 agggccagtc gctggcctgc gagctgcgcg tgcggccgcc ccggccggaa aagggtactt
      481 tttacgagat cgtgcgctgg caggtggaga gcgcgggcga atggcagccg gacgaggggc
      541 tgccggtcta cggcggcgac ggagccgccg gggccctgcc cggcaggccc tgatcttttc
      601 ctcttatggg gtttttactt gccgcctgag cggacgttaa atgtcggtcg tgcggatgga
      661 aagcgtctcc gtgggcggat gttacctgcc ggttgtgcgg gtggaaagcg cgtccgtggg
      721 cggatgtttc ctgccggttg ccgcgggtgg gcggcccggg gcacacggaa aattttgccc
      781 cccggcgcgc ccccgcaaac aggccgtttt ttcgccggcc ccgtcttccg cccctggaca
      841 agcccccttg ccgacagcct cctgttcaag agcccgcctt gcccgacggc ggaggcaggg
      901 cttttgtgcg ggcccccgcg gcggagccgc agtctttcaa ccgcaaagag acggacgggc
      961 gccgggccca accggcgcgc cgcccgtttt gatctctcca ccgaactcca cttgaaagga
     1021 aacagcatgg atatcaccgt acagctcaag gacgcggtgg cgcggggcgc ctccgatatc
     1081 ttcatcgtcg ccgggcggcc gctggtgtac aagctcaacg gccagctcac caacgtggac
     1141 gaagcgcggc tgctgccgcc ggacaccgag gagtttgtgc gccagatcta caagctggcc
     1201 aaggaccggc ccatggaccg gctgatggag accggcgacg acgatttttc ctttgcggtg
     1261 cagggcatct cccgctttcg ggtcagcgcc tacaagcagc gctccagcct ggcggcggtg
     1321 gtgcgggtca tcacctttga gctgccggac cccgcggcgc tgcacatccc cgagcaggtg
     1381 ctgcggctgg ccgaccgcac caagggcatg gtgctggtga ccggctccgc cggcagcggc
     1441 aaatccacca ccctcgcctg catgatcgac cgcatcaact gcacccgggc cgaccacatc
     1501 atcacgctgg aggacccgct ggaatacctg cacagccaca aaaagagcat cgtcagccag
     1561 cgggagaccg gcagcgacac cgagagctat ctcgccgccc tgcgcggggc gctgcgccag
     1621 agcccggacg tgattctgct gggcgagatg cgggactatg agaccatcac ggtggccatg
     1681 accgcggccg agacggggca tctgctcttt tccagcctgc acacggtggg ggccgccaac
     1741 accatcgacc gcattctgga cgtttttccc cccacccagc agcaccagat cgcggtgcag
     1801 ctggccatgg tgctgcaggc ggtggtgagc cagcagctgc tgcccaccgt ggacggcggc
     1861 ctgctgcccg cctttgagat catgaacgtc aaccccgcca tccgcaacat gatccgcgag
     1921 aacaagatcc atcagatcga cacggtgatc ggctcgtcgg cggacgagga catgatctcg
     1981 atggacgcca gcatcctgaa cctttaccgg gcgggcagga tcagcgagcg caccgcgctg
     2041 acctacgcgg cgaacccgga aattctggca aaaaagctca agctgtgaga gcggga
//
LOCUS       DBMDYA010000542         2091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11033_length_2091_cov_1.479397, whole genome shotgun sequence.
ACCESSION   DBMDYA010000542 DBMDYA010000000
VERSION     DBMDYA010000542.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_2091_cov_1.479397"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(666..893)
                     /locus_tag="ACZPFK_11680"
     CDS             complement(666..893)
                     /locus_tag="ACZPFK_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407011.1"
                     /translation="MPYPNIHAERGRRGMTMEALARSLGVTRKTLYNWMARGKVPPPK
                     LEKMARLFGCSADYLLGAPGQGQAPGQTQAE"
     gene            1085..1447
                     /locus_tag="ACZPFK_11685"
     CDS             1085..1447
                     /locus_tag="ACZPFK_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407012.1"
                     /translation="MIRLQELRAQKGITMKEAAARLGMPYTTYVNYEKGLREPSSETL
                     ILLADFYETTVDQLLGRDADTAAAAQGPQAQKERKLQLLARRAAGLPEAEYDRILKNF
                     EDTIELYLQARGIDPEGK"
     gene            1447..2040
                     /locus_tag="ACZPFK_11690"
     CDS             1447..2040
                     /locus_tag="ACZPFK_11690"
                     /inference="COORDINATES: protein motif:HMM:NF017885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1407013.1"
                     /translation="MADYARSQLAATDLLLRQQITGLAVEPERLAYDRNIRFDTLQGY
                     CAATGARLSDFVCGDEALRDGCTLRYGEFYLVLANSHGVANYKRRKFTLAHEVGHIYL
                     GHTWHGPEEEKEANWFASQLLMPECVLMEIWRRDGKLLAQDVAGWFFVSDAAAHNRVR
                     QLQRARRYRFGAAEKQLVQRYLPHILAALRDPYPVSV"
BASE COUNT          382 a          705 c          659 g          345 t
ORIGIN      
        1 tcggacaaga ccacaaggcc aaagccgcac gcctcccgcc tcttccgcga gggacccacg
       61 cgcgccggaa aaagcagcgc aagccccgca ccacgcctcc cgcctccctg gcaagaggga
      121 ttcccgcccc gcagagcgtc ggagggaggg gcaaagcgcc gctccgcccg ggaagaagcg
      181 gagttttcgt cccgccgcgt gaaaaacccg cgggagacgg aaaggacagt ggcgccgatc
      241 tgacccgaaa ggggaacgtg gcctttgtcc cgccgtgcga acctccctca agacggcccg
      301 cggccgccgg tcccctgaaa gggggccatg aaacgcgccg cctcggacaa gaccacaaag
      361 ccaaagccgc gcgccgcgtg tttcctccgg caagagagac gcgcgccgca gagagcagcg
      421 caaaccccgc accgctccgc ccgcctcccc ggcaaggggg aagcggctcc cttgccgatc
      481 tggaggctaa gggaggccaa ggtcttgccc gcactccccc accaagaggc caacgcgcgc
      541 tgtaaagacc ggcgcaggcc gtaaaccgcc acgcgcggtt cccgccggtc tctccgctcc
      601 cgttttcccg ttctttccgg ccctcccgcc gcgctcctcc gtggcccgga cggcttctgc
      661 cgccgtcact ccgcctgcgt ttgccccggc gcttgcccct gtccgggcgc gcccagcagg
      721 taatcggccg agcagccgaa cagcctggcc attttttcca gcttcggcgg gggcaccttc
      781 ccgcgcgcca tccagttgta aagcgtcttt ctcgtcacgc ccaggctgcg ggccagggcc
      841 tccatggtca tgccccggcg gccgcgctcg gcgtggatgt tgggatacgg catccgctca
      901 cctccttatg ttgatacccg ttatgggtac tggatctata gtatactcaa aatgagtatt
      961 tgtaaagcaa aataataccc gatttgagca aatttctttt gtgcatttcg ctcaaatcgg
     1021 gtatttccca ttgacaaatt actcataatg tgtatattat agagtcgaga aagaggtgac
     1081 cgctgtgatc cggctgcagg agcttagggc ccaaaagggc atcaccatga aagaggcggc
     1141 agcacgcctg ggcatgccct ataccaccta cgtcaactat gaaaaggggc tgcgcgagcc
     1201 cagctcggag acgctgatcc tgctggcgga tttttacgag accacggtgg accagctgct
     1261 ggggcgggac gcggacaccg cggcggccgc gcagggcccg caggcccaaa aagagcgcaa
     1321 gctgcagctg ctggcgcggc gggccgccgg cctgccggag gccgagtacg accgcatttt
     1381 gaaaaatttt gaggacacga tcgagctcta cctgcaggcg aggggcatcg atccggaggg
     1441 caaataatgg ctgattacgc ccgcagccag ctggccgcca ccgacctgct gctgcgccag
     1501 cagatcaccg gtttggccgt ggagccggag cggctggcct acgaccgcaa catccgtttt
     1561 gacacgctgc agggctattg tgcggccacc ggggcgcggc tgtccgattt tgtctgcggc
     1621 gacgaggccc tgcgggacgg ctgcaccctg cgctacggcg agttttatct ggtgctggcc
     1681 aactcgcacg gggtggccaa ctacaagcgc cgcaagttca cgctggccca cgaggtgggc
     1741 catatctatc tgggccacac ctggcacggc ccggaggagg aaaaagaggc caactggttc
     1801 gccagccagc tgctgatgcc cgagtgcgtg ctgatggaga tctggcgccg ggacggcaag
     1861 ctgctggcgc aggacgtggc gggctggttc tttgtctcgg acgcggcggc gcacaaccgg
     1921 gtgcgccagc tgcagcgggc gcgccgctac cgcttcggcg ccgcggagaa gcagctcgtc
     1981 cagcgctatc tgccccacat cctggccgcc ctgcgcgacc cctatcccgt gtcggtgtag
     2041 ccggggcgcg tttgccgaag aggcgtttta ttcttccctt accgggccgc g
//
LOCUS       DBMDYA010000543         2090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11039_length_2090_cov_1.246858, whole genome shotgun sequence.
ACCESSION   DBMDYA010000543 DBMDYA010000000
VERSION     DBMDYA010000543.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11039_length_2090_cov_1.246858"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="ACZPFK_11695"
     CDS             complement(<1..1155)
                     /locus_tag="ACZPFK_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1407014.1"
                     /translation="MRRTEKTDKRKPVERLQPDPAAGITFAQAAQRTARGWDNRAAAP
                     LSKTTWQIVRDNVFTPFNLLFCVLAACLFAVGSYSNMMFLGIVVCNTLIGIIQELRVK
                     RELSKITLMAAPTAQVVREGQELRLPVEELVLDDVVRLAAGCQIPADAVLLQGALEVN
                     EALLTGEADNVAKVPGDRLLSGSFVVSGRGAARLDAVGADAFAARITHEAKRTKKNRS
                     EMMRSLDKLLRWIGLALIPVAVILFVKQYTLLHTGLEYAVTSTVGAVVGMIPEGLYLL
                     TSLALAVGVLNLARRRTLVHELSCIENLARVDVLCLDKTGTLTEGSMEVLKAMPLAQG
                     LDGPGLEALLGSFVHSGSDDNATARALKERFPDGGLLAGAEEIAFSSARKW"
     gene            complement(1736..>2090)
                     /locus_tag="ACZPFK_11700"
     CDS             complement(1736..>2090)
                     /locus_tag="ACZPFK_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR1407015.1"
                     /translation="LSLSSRSVMGVELPTTALARRPLKPLYSFGNTNAALDYAYLCFD
                     RVKQLTARLASIDNSVYRLAVAIKKTQRRANMLENVNIPKFEENIRFISGVLEERERE
                     EFTRLKVIKRTKGKS"
BASE COUNT          366 a          696 c          685 g          343 t
ORIGIN      
        1 ccacttgcgg gcggaggaaa aggcgatctc ctctgcgccc gccaacaggc cgccgtcggg
       61 aaagcgctct ttcagcgcgc gggcggtggc gttgtcgtcg ctgccgctgt gcacaaagct
      121 gcccagcagc gcctccagcc ccgggccgtc cagcccctgt gccagcggca tggctttcag
      181 cacctccatg ctgccctcgg tgagggtgcc ggtcttgtcc aggcagagca cgtccacccg
      241 ggccagattt tcgatgcagg acagctcgtg caccagcgtg cggcggcggg ccaggttgag
      301 cacccccacg gccagggcca ggctggtgag caggtaaagc ccctcgggga tcatgcccac
      361 caccgccccc accgtggagg tgacggcgta ttccaggccg gtgtgcagca gcgtgtactg
      421 cttgacgaac aggatgaccg ccaccgggat cagggccagc ccgatccagc gcagcagctt
      481 gtcgagcgag cgcatcatct cggagcggtt cttcttggtg cgcttggcct cgtgggtgat
      541 gcgggccgca aaggcgtccg cgcccaccgc gtccagccgc gccgcgccgc ggcccgacac
      601 cacaaagctg cccgagagca gccggtcccc cgggaccttg gccacattgt ccgcctcgcc
      661 ggtgagcaga gcctcgttga cctccagcgc gccctgcagc agcaccgcgt cggcggggat
      721 ctggcagccg gccgccaggc gcaccacgtc gtccagcacc agctcttcca ccggcagccg
      781 cagctcctgc ccctcccgca ccacctgggc ggtgggggcg gccatcagcg tgatcttgga
      841 cagctcccgc ttgacccgca gctcctggat aatgccgatg agggtgttgc agaccacgat
      901 gcccagaaac atcatattac tataagagcc caccgcaaac aggcaggcgg ccagcacgca
      961 gaacagcagg ttgaacgggg tgaacacatt gtcccgcacg atctgccagg tggttttgga
     1021 gagcggggcc gccgcccggt tgtcccagcc ccgtgcggtg cgctgggccg cctgggcaaa
     1081 ggtgatgccc gccgccgggt ccggctgcag ccgctccacg ggtttgcgct tgtccgtttt
     1141 ctctgtccgt ctcatcactg cgattcctct tccgttttgt ttctacccat aatacgagcc
     1201 gcgggcgcgg cgggttccaa agaaaatcta aagaatctgt tacgatttgt gaaacagccc
     1261 gctggaaaag cgggcaaagc ggcgcaaaag gccgccggcg ggcgaggggc cgggggcgct
     1321 catgattcca gtgtctgcaa aaaagccgcc tccatgcggc gcggcgaaaa agaaaaacgg
     1381 ccccgcccga agcgcagtgg ggcggggaaa aatcggtttt gcccggcaag gcaaaaccgc
     1441 gcagcatcgg ctggttgcgg aaaaggttcg gctgcgagaa agcagcgcgc cgcaggcccc
     1501 tatcacctgc aggcggtggg gacgcagcag acaggctctt ccgtcacaaa caggcgacgg
     1561 ttcttccctg tcggcactgc gcgaaagagg ccggacggga tggttttgat atgtccgaca
     1621 tcgctccaag cgcggcgata ccgccggccg gtcgcggcgg cctgcagcgg gggcagcggc
     1681 caaggggcaa aacaaacgcg cccgggcagc cagcccgggc gcgttgccgt agttttcagc
     1741 tctttccctt ggtcctcttg atgaccttga ggcgggtgaa ctcctcccgc tcccgctcct
     1801 ccagcacgcc ggagataaag cggatgttct cctcaaattt cgggatattc acgttttcca
     1861 gcatattggc gcggcgctgg gtctttttga tggccaccgc cagccggtag acgctgttgt
     1921 cgatgctggc cagccgcgcc gtcagctgct tgacgcggtc aaaacacagg taggcgtagt
     1981 ccagcgcggc gttggtgttg ccaaagctgt acagcggctt cagcgggcgg cgggccagcg
     2041 ccgtggtggg cagctccacc cccatgacgc tgcgggagga cagcgagagc
//
LOCUS       DBMDYA010000544         2072 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11150_length_2072_cov_1.413496, whole genome shotgun sequence.
ACCESSION   DBMDYA010000544 DBMDYA010000000
VERSION     DBMDYA010000544.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_2072_cov_1.413496"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="ACZPFK_11705"
     CDS             complement(<1..468)
                     /locus_tag="ACZPFK_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1407016.1"
                     /translation="MRKTKIICTLGPSTDSEETLRQMVAAGMNVARFNFSHGTYEEHL
                     ARLALLRKVRNEAEEPIAALLDTKGPEIRLRDFKGGKVFLKAGSTFVLTTREVEGTEE
                     ICSITYRDLPQDVKVGTRLLLDDGLISMVVSKIEGTEITCAVQNSGYVSNHKSV"
     gene            443..682
                     /locus_tag="ACZPFK_11710"
     CDS             443..682
                     /locus_tag="ACZPFK_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407017.1"
                     /translation="MQMILVLRKAQTSLSSGGGNCFPSLFTESEILYTIPPCLPTAFS
                     VDFTFQELFGQKNRERENRKRERSEQNGEKMVVFV"
     gene            800..1975
                     /locus_tag="ACZPFK_11715"
     CDS             800..1975
                     /locus_tag="ACZPFK_11715"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1407018.1"
                     /translation="MAKRAFLIVLDSFGIGAEPDAAAFGDEGTNTLGAIAAHPNFNCP
                     NLQKLGMFNIDGVTAGERCAAPAGAFARLQEQSMGKDTTIGHWEIAGVVSPEPLPTFP
                     DGFPAELIAEYEAKTGHKVLCNKPYSGTQVLKDYGEQAIAENALIVYTSADSVFQVAA
                     SEELVPVPELYRYCEIAREMLTGKYGVGRVIARPFLGKTADTFYRTTNRHDLSLKPPR
                     ATMLDVLKEAGKDVIAVGKIYDIFDGEGVTEKIKTTGNTNGIAFTKALQTRDFEGLAF
                     VNLVDFDMLYGHRRDVAGYAAAASEFDAFLSEFIPGMREDDILMVTADHGCDPSYTKT
                     TDHTREYVPLLICGKRVKEGADLGTRMGFGTIARTICDYLGVDNQLDGESVLPEILK"
BASE COUNT          440 a          644 c          610 g          378 t
ORIGIN      
        1 cacactttta tggttggaga cgtagccgct gttctgcacc gcgcaggtga tctcggtgcc
       61 ctcgatcttg gacaccacca tcgagatcag gccgtcgtcc agcagcagac gggtgccgac
      121 tttcacgtcc tggggcaggt cgcggtaggt gatggaacag atctcctcgg tgccctccac
      181 ctcccgtgtg gtcagcacaa aggtggagcc cgctttcaaa aagacctttc cgcccttaaa
      241 atcccgcagg cggatctccg gccccttggt gtccagcaga gcggcgatgg gctcctccgc
      301 ctcgttgcgc acctttcgca gcagagcgag acgggcgaga tgctcttcat aggtaccgtg
      361 agaaaaattg aaacgggcga cattcatgcc cgcggccacc atttggcgca gcgtttcctc
      421 cgaatcggtg gaggggccga gggtgcagat gatcttggtc ttacgcaaag cgcaaacctc
      481 cttatcaagc ggcgggggaa attgcttccc ctctcttttt acggaatcag aaatattata
      541 caccatcccg ccctgtttgc ctacagcctt ttctgtcgat tttacatttc aggaactttt
      601 tggtcaaaaa aacagggaaa gagaaaaccg gaaaagggag cggagcgagc aaaacggtga
      661 aaaaatggtt gtctttgtgt gaaaaacaga ggaaagaaag caaaccgcat tgtccgcgcc
      721 tttcttttat gatacaataa agccaccgga tctgatgatc ggcagaacct atcattttat
      781 gatataggag cgtagcaaaa tggcaaaaag agcatttctc atcgtactgg acagctttgg
      841 catcggcgcg gagccggacg ccgccgcttt cggcgacgag ggcaccaata ccctgggcgc
      901 cattgcggcc caccccaact tcaactgccc caatctgcaa aagctgggca tgttcaacat
      961 cgacggcgtg acggcgggcg aaaggtgcgc cgccccggcc ggggctttcg cccgcctgca
     1021 ggagcagagc atgggcaagg acaccaccat cggccactgg gagatcgccg gcgtcgtcag
     1081 ccccgagccg ctgcccactt tcccggacgg attccccgcc gagctgatcg ccgagtatga
     1141 ggccaagacc ggccacaagg tgctgtgcaa caagccctat tccggcaccc aggtcctcaa
     1201 ggattacggc gagcaggcca tagcggagaa cgccctcatc gtctatacca gcgcggacag
     1261 cgtgttccag gtggcggcca gcgaagagct ggtgccggtg ccggagttgt accgctactg
     1321 cgagatcgcc cgcgagatgc tcacgggcaa gtacggcgtg ggccgggtca tcgcccgccc
     1381 gttcctgggc aagacggcgg acacgttcta ccgcaccacc aaccgtcacg acctgagcct
     1441 gaagccgccc cgcgccacca tgctggacgt gctgaaagag gccggcaagg acgtgatcgc
     1501 ggtgggcaag atctacgaca tcttcgacgg cgagggcgtg accgaaaaga tcaagaccac
     1561 cggcaacacc aacggcatcg ctttcaccaa ggcgctgcag acccgtgact tcgagggcct
     1621 ggcctttgtc aacctggtgg acttcgacat gctgtacggc caccgccgcg acgtggcggg
     1681 ctatgccgcg gccgccagcg aattcgacgc ttttctgtct gagttcatcc ccggcatgcg
     1741 ggaggacgat atcctgatgg tcaccgccga ccacggctgc gacccctctt acaccaagac
     1801 caccgaccac acccgcgagt acgtgcccct gctgatctgc ggcaagcggg tcaaggaggg
     1861 cgccgacctg ggcacccgca tgggctttgg caccattgcc cgcaccatct gcgactatct
     1921 cggcgtcgac aatcagctgg acggcgagag cgtgctgcct gaaattttga aataagccgc
     1981 cgcgcggcag agggccgtcc catcggaaca gcgatagggg gcggccctct ttttttgtcc
     2041 ctcctgcctg cgggggcgcc gcccgcgggc ag
//
LOCUS       DBMDYA010000545         2070 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11162_length_2070_cov_1.605891, whole genome shotgun sequence.
ACCESSION   DBMDYA010000545 DBMDYA010000000
VERSION     DBMDYA010000545.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11162_length_2070_cov_1.605891"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            680..>2070
                     /locus_tag="ACZPFK_11720"
     CDS             680..>2070
                     /locus_tag="ACZPFK_11720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1407019.1"
                     /translation="MLQLILGTGGSGKTTELLQRVRQRAGQGKRCLYLVPEQFSQTAE
                     LLLYDALGDRLSAFAEAVSFRTLAERIERECGGQALPTLTDAGRCVFVRRAITSLGEE
                     VRYYRRHRHNTAFLAECAAAIGELKTAGASSGLLADAAARAGSEKLAELSLIYAATEA
                     AMEGKAIDPSDRVTRAAEKLSPGLFAETWFYVDDFDGFTAPQYALLRRLLPWAEGVTV
                     TLCCDGLSDAEGGFGLFSPVKKTAQRLIRLAKAEGEQVQTPIVLRGNRRAAKPGLAAV
                     EALLTGGAGDAPLPSEGVTLDRPATREEECARVAASIRALAEDGTRYSEIAVVCRLLE
                     DFRAPLQAALARQQIPFFFDRSVTAEYTAPVAFLRAALGLARGGVSTRYLLALLKTGL
                     CGVPVEAVSALENYAFTWQPSAAAWREPFTLNPAGLGRELRPEDEAELALAETARRAV
                     MGPVGDFLEHVKNP"
BASE COUNT          328 a          645 c          734 g          363 t
ORIGIN      
        1 accctgcaaa tccgccaggc cgggcataga agattgcccg gcctgccttg tggcaccggc
       61 ggatttgatt ggctgagttt tgcgcttggg ggtcgcccgc agggtacccc cacagctgaa
      121 atgatagtgg gcgaccctgc aaatccgcct ggccgggcat agaagattgc ccggcctgcc
      181 ttgtggcacc ggcggatttg attagctgag ttttgcgctt ggggtcgccc gcagagtacc
      241 cccacagctg aaatgaccca aggttaaaga aaagctcggg agaatggcgc agccattctc
      301 acaggggtgc gcagggagac cagagcggaa gtataagata ccaacgcatc cccagccttt
      361 gcggcaccgg cggatttggt cggctgagct ttgcgcttgg ggtcgcccgc agggtacctc
      421 cacagctgaa atgacccgag gttaaaggaa agctcggcag aatggcgcac ccatcctcac
      481 agatgcgcgc gggtgaaaag tgcggggcgg cgggaccgct ccgccgcgcc aggggcggaa
      541 ctcgcaggcc tgcagtcagg cttcttccat ttcctgtcat tgaccttttt cgggccaacg
      601 tttacaataa aaccagcggc aaaagccgct ggttttgttt tttcatctct tttatcaaat
      661 aaaaaagggg gcggcgcttt tgttacagct tattttgggg acgggcgggt ccggcaagac
      721 caccgagctg ctgcaacggg tccggcagag ggcggggcag ggcaagcgct gcctgtatct
      781 tgtgcccgag cagttcagcc agacggcgga gctgctgctc tacgacgcac tgggcgaccg
      841 cctctccgcc tttgcggagg cggtgtcgtt ccgcacgctg gccgagcgca ttgagcggga
      901 gtgcggcggc caggcgctgc ccaccctcac cgacgccggc cgctgtgtct ttgtgcggcg
      961 ggccatcacc tcgttggggg aggaggtgcg ctattaccgc cgccatcgcc acaacaccgc
     1021 gtttttggcc gaatgtgccg ccgccatcgg cgagctgaag accgccgggg cctccagcgg
     1081 tttgttggcg gacgccgccg cgcgggcggg cagtgaaaag ctggccgagc tctcgctgat
     1141 ctacgccgcc accgaggcgg cgatggaggg caaggccatc gaccccagcg accgggtcac
     1201 ccgcgcggcg gaaaagctgt cgccggggct gtttgcggag acttggtttt atgtggacga
     1261 cttcgacggc tttaccgccc cccagtatgc gctgctgcgg cggctgctcc cctgggcgga
     1321 gggggtgacg gtgaccctct gctgtgacgg cctcagcgac gcggagggcg gtttcggcct
     1381 gttcagcccg gtcaaaaaga cggcgcagcg gctgatccgt ctggcaaagg ccgagggcga
     1441 gcaggtgcag acgcccatcg tgctgcgcgg caaccggcgc gcggcgaagc ccggcctcgc
     1501 ggcggtggaa gcgctgctca ccggcggagc gggcgatgcg cccctgccct cagagggggt
     1561 cacgctggac cggcccgcca cccgtgagga ggagtgcgcg cgggtggcgg cgtcgatccg
     1621 cgctttggcc gaggatggga cgcgctacag cgagatcgcc gtcgtctgcc gcctgctgga
     1681 ggatttccgc gccccgctgc aggcggcgtt ggcgcgtcag cagatcccgt ttttctttga
     1741 ccgctcggtc acggccgagt acaccgcccc tgtggcgttc ctgcgggcgg cgctggggct
     1801 ggcgcggggc ggcgtttcca cccgctacct gctggctctg ctcaagaccg gtctgtgcgg
     1861 cgtgcccgtg gaggcggtgt cggcgctgga aaattacgcc tttacctggc agcccagcgc
     1921 ggccgcctgg cgggagccgt ttaccctgaa ccccgccggc ctggggcggg agctgcggcc
     1981 ggaggacgag gccgagctgg cgctggctga gacagcccgc cgggcggtga tggggccggt
     2041 gggcgatttt ctcgagcatg tcaaaaaccc
//
LOCUS       DBMDYA010000546         2060 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11225_length_2060_cov_1.191424, whole genome shotgun sequence.
ACCESSION   DBMDYA010000546 DBMDYA010000000
VERSION     DBMDYA010000546.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11225_length_2060_cov_1.191424"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="ACZPFK_11725"
     CDS             <1..582
                     /locus_tag="ACZPFK_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIR1407020.1"
                     /translation="VLEGRSLPDPERPGYFAIAPVHEATLGWLADTILSFADSRATLQ
                     VPDLSDPLTKKLYSTYLSYLEPERGFKYPLEMHEDARGSFTEFLRSPDRGQVSVNVSR
                     PGIVKGNHWHDTKNEKFLVVSGRACIRFRRPDGDAVYEYHVCGDKLEVVDIPTGYTHS
                     IENEGEGDLVTVMWANECFDPDRPDTWFLPVEG"
     gene            649..1776
                     /gene="wecB"
                     /locus_tag="ACZPFK_11730"
     CDS             649..1776
                     /gene="wecB"
                     /locus_tag="ACZPFK_11730"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR1407021.1"
                     /translation="MKKLKLMTIVGTRPEIIRLSEVIKCCDKYFDHLLVHTGQNYDYR
                     LNGIFFEDLGLRQPDYYLDCVGKDLGETMGGIIAKSYALMARQRPDAVLLLGDTNSAL
                     SAISAKRLKIPIFHMEAGNRCWDWNVSEMINRKIVDHISDINLPYTEHSRRYLLSEGL
                     DGKTIFVTGSPMREVLAAHAEAIENSPVLEQLGLEAGSYILLSAHREENIDNEENFMS
                     LMGAVNRIAGHTGMPVIYSCHPRSRKYIEARRFQFHPLVREMQPFGFLDYNKLQKNAY
                     CVLSDSGTLSEESAMLGFAGVLIRTSTERPEVLDKGSVVIGGIKGDDVEQAMELAVAM
                     RDNAEPVVMAADYADTNVSVKVVKLIQSYAKIVNETVWLKR"
     gene            1868..>2060
                     /locus_tag="ACZPFK_11735"
     CDS             1868..>2060
                     /locus_tag="ACZPFK_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1407022.1"
                     /translation="MAKRILVVSQHYWPETFRSTDVCQGFAELGYEVDVLCGLPNYPK
                     GELAPGYGFFKRRREARGGV"
BASE COUNT          394 a          672 c          663 g          331 t
ORIGIN      
        1 gtgctggagg ggcgcagcct gcccgacccc gagcgcccgg gctatttcgc catcgccccg
       61 gtgcacgagg ccacgctggg ctggctggcg gacaccatcc tctctttcgc cgacagccgc
      121 gccaccctgc aggtgcccga cctctcggac ccgctgacga aaaagctcta cagcacctat
      181 ctgtcctacc tggagccgga gcgcggcttt aaatacccgc tggagatgca cgaggacgcg
      241 cgcggcagct tcaccgagtt tctgcgcagc ccggaccggg gccaggtgag cgtcaacgtc
      301 agccgccccg gcatcgtcaa gggcaaccac tggcacgaca ccaaaaacga aaagttcctg
      361 gtcgtttccg gcagggcctg catccgcttt cgccggccgg acggcgacgc cgtctacgag
      421 taccacgtct gcggggacaa gctggaggtg gtggacatcc ccaccggcta cacccactcg
      481 atcgaaaacg agggcgaggg cgacctggtg accgtgatgt gggccaacga gtgctttgac
      541 cccgaccgtc cggacacctg gtttttgccg gtggaggggt gagggtggcc cgcgttttgc
      601 cctttgccct gatttgacca aaccgcccga acggaggctg ttgtaaaaat gaaaaagctc
      661 aagctcatga ccatcgtcgg cacccggccc gagatcatcc ggctgtcgga ggtcatcaaa
      721 tgctgcgata aatacttcga ccatctgctg gtgcacaccg ggcagaacta cgattaccgc
      781 ctgaacggca tcttttttga ggacctgggc ctgcgccagc cggactatta cctggactgc
      841 gtgggcaagg acctgggcga gaccatgggc ggcatcatcg ccaagagcta tgcgctgatg
      901 gcccggcagc ggcccgacgc ggtgctgctg ctgggcgaca ccaacagcgc cctgtccgcc
      961 atctcggcaa agaggctgaa gatccccatc ttccacatgg aggctggcaa ccgctgctgg
     1021 gactggaacg tgtccgagat gatcaaccgc aagattgtgg accacatcag cgacatcaac
     1081 ctgccctaca ccgagcactc ccgccgctac ctgctcagcg aggggctgga cggcaagacg
     1141 atctttgtca ccggcagccc catgcgcgag gtgctggccg cccacgccga ggccatcgag
     1201 aacagccccg tgctggagca gctgggcctc gaggcgggca gctacatcct gctctcggcc
     1261 caccgggagg agaacatcga caatgaggaa aatttcatgt ccctgatggg ggcggtgaac
     1321 cgcatcgccg ggcacaccgg catgccggtg atctactcct gccacccgcg cagccgcaag
     1381 tacatcgagg cccggcgctt ccaatttcac ccgctggtgc gggagatgca gcccttcggc
     1441 tttctcgatt acaacaagct gcagaagaac gcctactgcg tgctgtccga ctccggcacc
     1501 ctgtcggagg agagcgccat gctgggcttt gccggggtgc tcatccgcac cagcaccgag
     1561 cggcccgagg tgctggacaa gggcagcgtg gtgatcggcg gcatcaaggg cgacgacgtg
     1621 gagcaagcca tggagctggc cgtggccatg cgcgacaacg ccgagccggt ggtgatggcg
     1681 gcggactacg ccgacaccaa cgtgtcggtc aaggtggtca agctgatcca gagctatgcc
     1741 aagatcgtca acgagacggt ctggctcaag cggtaggccg cgacgaaaaa agcggaggcg
     1801 ggcggggccg gcggcatatc gccgtccggg gccgcgctct gccccgcgag caaaaggtgg
     1861 tgcctttttg gcaaaacgca tcctggtcgt cagccagcac tactggcccg agacctttcg
     1921 cagcaccgac gtctgccagg gcttcgccga gctgggctac gaggtggacg tgctctgcgg
     1981 gctgcccaac taccccaagg gcgagctggc gcccggctac ggctttttca agcgccgccg
     2041 ggaggcccgc ggcggggtgg
//
LOCUS       DBMDYA010000547         2057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11243_length_2057_cov_1.486196, whole genome shotgun sequence.
ACCESSION   DBMDYA010000547 DBMDYA010000000
VERSION     DBMDYA010000547.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11243_length_2057_cov_1.486196"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="ACZPFK_11740"
     CDS             complement(<1..586)
                     /locus_tag="ACZPFK_11740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR1407023.1"
                     /translation="MNIHGKDIKIFAGNSNKDLAISIAECLGLPLGKSEIGMFSDGEI
                     SVSIGESVRGSDVFVVQSTSSPVNRHLMELLLMIDAFRRASAGRITAVIPYYGYARQD
                     RKNKARDPISAKLVADLICKAGADRVLTMDLHAAQIQGFFDVPVDHMLGMPILAAYYE
                     KKIAEEKDKDFSVVSPDLGSVTRARTFAERLGLPL"
     gene            complement(664..1425)
                     /locus_tag="ACZPFK_11745"
     CDS             complement(664..1425)
                     /locus_tag="ACZPFK_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HIR1407024.1"
                     /translation="MSENTGSARSRYEAARAAFDAAFERHLDAGVEFISKDGVCIDPE
                     VEIAPGVLILPGTILRGKTVIGAGSVIGPDSLLEDAVVGENVTFNASQGRACRIGDGA
                     TIGPWVQLRPDSVIGKNVHIGDFVEIKNSTIGEGTAVAHLTYIGDADVGRYCNFGCGV
                     VVVNYDGEVKSRTSIGDYCFIGCNTNLVAPVRVGDGAYTAAGSTITEDVPPEALGIAR
                     ARQVTKEGWAGPKLAAYIAKKQKLEAAARGREGEK"
     gene            1799..>2057
                     /locus_tag="ACZPFK_11750"
     CDS             1799..>2057
                     /locus_tag="ACZPFK_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR1407025.1"
                     /translation="MSKRFLYLFKEGHEAFGGDQTKMKNILGGKGAGLAEMTNAGMPV
                     PQGFTITTEACTQYYADGREINEEIKADIFKYMGLLEEITGK"
BASE COUNT          376 a          630 c          631 g          420 t
ORIGIN      
        1 caaggggcag gcccagccgc tcggcaaagg tgcgcgcgcg ggtgacggag cccaggtcgg
       61 gggagaccac gctaaagtct ttgtcctttt cttcggcgat ctttttttcg taataggcgg
      121 ccaggatcgg catgcccagc atgtggtcca ccggcacgtc gaaaaagccc tggatctgcg
      181 ccgcgtgcag gtccatcgtc agcacccggt cggcgccggc cttgcagatc aggtcggcca
      241 ccagcttggc gctgatgggg tcgcgggcct tgttcttgcg gtcctgccgg gcatagccgt
      301 aataggggat gacggcggtg atgcgccccg cggaggcgcg ccggaacgcg tcgatcatca
      361 gcagcagctc catcaggtgg cggttgaccg ggctggaggt gctctgcacc acaaagacgt
      421 cgctaccgcg gaccgactcg ccgatggaga cgctgatctc gccgtcagag aacatgccga
      481 tctcggattt gcccagcggc aggcccaggc attcggcgat ggagatggcc aggtccttgt
      541 tggagttgcc cgcgaagatc ttgatgtctt ttccgtggat gttcatttta tttcccccgt
      601 ttatataatg tagagcgcgt gttgccccgc ttgccgtctg cggcccgggg ccgttttttc
      661 aagctacttt tccccctcgc ggccgcgggc cgccgcctcc agcttttgtt tttttgcgat
      721 gtaggcagcc aacttgggtc ctgcccagcc ctctttggtc acctggcgcg cccgggcgat
      781 gcccagcgcc tcggggggca catcctcggt gatggtgctg cccgccgccg tgtaggcgcc
      841 gtcgccgacc cgcaccgggg ccaccaggtt ggtgttgcag ccgataaagc agtagtcgcc
      901 gatggaggtg cggctcttga cttcgccgtc gtagttgacc accaccacgc cgcagccgaa
      961 attgcagtat ctgcccacgt cggcgtcgcc gatgtaggtc agatgggcca ccgcggtgcc
     1021 ctcgccgatg gtggagtttt tgatctcgac aaagtcgccg atgtgcacgt ttttaccgat
     1081 gacgctgtcc ggccgcagct gcacccaggg gccgatggtg gccccgtccc cgatgcggca
     1141 ggcgcgcccc tggctggcgt tgaacgtgac gttctcgccc accaccgcgt cctccagcag
     1201 gctgtcgggg ccgatcacgc tgcccgcgcc gatcaccgtt ttgccccgca ggatggtgcc
     1261 gggcaaaata agaaccccgg gtgcgatctc cacctcaggg tcgatacaga cgccgtcctt
     1321 gctgataaat tccacccccg cgtccagatg gcgctcaaag gcggcgtcga acgcggcgcg
     1381 cgcggcctcg tagcggctgc gcgcggaacc agtattttcg ctcatggcaa ctcctccggt
     1441 ttggacattt gaagcctcgg ggatggcgca aaatatgttt ttcgacgaaa aacagccggc
     1501 gggcctctgc ccgctgggcc atccttacat ctatcatttt acaggttcca caagtttttt
     1561 caagggcccc cggtcaaaca tcatggaaaa tagataattt tatcaatttc ccataaataa
     1621 gtatggtaaa ccaaaaaaaa gtttgttata attatggtgc gactggacag gccctttccg
     1681 ggcctgtttt ggtgttctgc gcggccccgc cgcagacggg gaagctcccg cgcggcgcgg
     1741 ttccggcggg aagcgcgccg ggaagaaacc tgcaattttt ttacggaggt agataaattt
     1801 gagcaaacga ttcctgtacc tgttcaaaga gggccacgag gctttcggcg gcgaccagac
     1861 caagatgaaa aacatcctgg gcggcaaggg cgccggcctg gccgaaatga ccaacgcggg
     1921 tatgcctgtc ccccagggct tcaccatcac caccgaggcc tgcacccagt actatgcgga
     1981 cggccgtgag atcaacgagg agatcaaggc cgacatcttc aagtatatgg gcctgctgga
     2041 ggagatcacc ggcaaaa
//
LOCUS       DBMDYA010000548         2055 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11254_length_2055_cov_1.124360, whole genome shotgun sequence.
ACCESSION   DBMDYA010000548 DBMDYA010000000
VERSION     DBMDYA010000548.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11254_length_2055_cov_1.124360"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            124..>2055
                     /locus_tag="ACZPFK_11755"
     CDS             124..>2055
                     /locus_tag="ACZPFK_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1407026.1"
                     /translation="MPEWTREQEIAIQDAGGALLVSASAGSGKTAVLVERALRLIAGP
                     DPVDADRLLILTFSNAAAAELRSRLGLKLEERIRSQPADLKLRRQQMLLQRASISTVS
                     AFCMQLLREHFSSLDIPPDFAVADDAAVYELRQSVLAEVLEEAYQKPDFAAFASIFGR
                     ARSDRQAGEALLAVYDHLRALPFFDRALAQLLALYDHSGPVAESPWGAELLGGAQQGA
                     EAGLALCDAALRVVDGEPALAAYRPALEGDRACFERARALLAAGDWDGARALLSGYLP
                     GKLSPVRGFDGPEKQLAQELRAGAKAQMAAILEDQLLCGEEEFRQDVAAARPLVAALC
                     EAVRLFDERFFAAKLADKVLEFSDFEHLALRLLAGEDGAPTPLAAEVRGRFDAVMVDE
                     YQDTNAIQDLLYRLLARPDGSNLFFVGDVKQSIYRFRKAMPEIFIEKKDRFAPYDGVH
                     YPASLILEHNFRSSKGVIEGINYFFSQLMSRQVGELDYCGGELLLPGSAAQDTGLPGA
                     ELLVVDTGENPAFGEADAVAELIVRMVREGARVRGGDGALRPCGFGDFCILLRSPRGA
                     AAAFADALAARGVPAHTDLGDGILTLPEVQPLIAALRVLDNPAQDVPLAALMTSPMFD
                     FSSAELARLRAETPEGSLFG"
BASE COUNT          280 a          682 c          770 g          323 t
ORIGIN      
        1 gccggccctg cgactggtgc gattaccgca gcatctgccg ccatgtggac ggcagaaacg
       61 agcggcaggt cgccgcgccg aaagacgcct ttgaggcgga gagcggagag gaggggagcg
      121 ccgatgcctg agtggacccg agaacaagag atcgccatcc aggacgcggg cggcgccctg
      181 ctggtctccg cctccgcggg cagcggcaag acggcggtgc tggtggagcg cgccctgcgg
      241 ctgatcgccg ggccggaccc ggtggacgcg gaccggctgc tgatcctcac cttttccaac
      301 gcggccgcgg ccgagctgcg cagccgcctg ggcctcaagc tggaggagcg catccgcagc
      361 cagcccgcag acctgaagct gcgccgccag cagatgctgc tgcagcgggc cagcatctca
      421 acggtcagcg ctttttgcat gcagctgctg cgggagcatt tcagcagcct ggacatcccg
      481 cccgactttg cggtcgcgga cgacgcggcg gtctacgagc tgcgccagtc ggtgctggcc
      541 gaggtgctgg aggaggcgta ccaaaagccg gattttgccg ctttcgcctc catcttcggc
      601 cgcgcccgca gcgaccggca ggcgggggag gcgctgctgg cggtctatga ccatctgcgg
      661 gcgttgccgt tttttgaccg ggcgctggcc cagctgctgg ccctgtacga tcacagcggc
      721 ccggtggcgg aaagcccctg gggggccgag ctgctgggcg gcgcacagca gggggccgag
      781 gccggccttg ccctctgcga cgcggcgctg cgggtggtgg acggcgagcc cgccctggcc
      841 gcctaccgcc ccgccctgga gggggaccgg gcctgctttg agcgggcgcg ggcgctgctg
      901 gcggcggggg attgggacgg cgcgcgggcg ctgctgtccg gctatctgcc gggcaagctg
      961 tcgccggtgc gcggcttcga cggccccgaa aagcagctgg cgcaggagct gcgcgccggc
     1021 gccaaggccc agatggcggc gatcctggag gatcagctgc tctgcggcga ggaggagttc
     1081 cggcaggacg tggccgccgc gcggccgctg gtggcggccc tctgcgaggc ggtgcgcctg
     1141 ttcgacgagc gctttttcgc cgccaagctg gcggacaagg tgttggaatt ttccgacttt
     1201 gaacatctgg cgctgcggct gctggcgggg gaggacggcg cgcccacccc gctggccgcc
     1261 gaggtgcgcg gccggttcga cgcggtgatg gtggacgaat accaggacac caacgccatc
     1321 caggacctgc tctaccgcct gctggcacgc cccgacggct ccaacctgtt ttttgtgggc
     1381 gacgtcaagc agagcatcta ccgcttccgc aaggcgatgc ccgagatttt catcgaaaaa
     1441 aaagaccgct ttgcccccta tgacggtgtg cattacccgg ccagcctgat tttggagcac
     1501 aatttccgct cctccaaagg ggtcatcgag ggaatcaact atttcttttc ccagctgatg
     1561 agccggcagg tgggcgagct ggactactgc ggcggcgagc tgctgctgcc gggctctgcg
     1621 gcgcaggaca ccggcctgcc cggcgcggag ctgctggtgg tggacaccgg cgagaacccg
     1681 gcctttggcg aggcggacgc ggtggcggag ctgatcgtgc gcatggtgcg cgagggcgcc
     1741 cgggtgcgcg gcggcgacgg cgcgctgcgc ccctgcggct tcggcgattt ctgcattttg
     1801 ctgcgctctc cgcgcggggc ggcggcggcc tttgccgacg cgctggcggc gcggggcgtg
     1861 cccgcccaca ccgatctcgg cgacggcatc ctcaccctgc ccgaggtgca gccgctgatc
     1921 gccgccctgc gggtgctgga caatccggcg caggacgtgc cgctggcggc gctgatgacc
     1981 tcgccgatgt tcgatttttc cagcgcggag ctggcgcgcc tgcgggccga gacgcccgag
     2041 ggcagcctgt tcggc
//
LOCUS       DBMDYA010000549         2050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11287_length_2050_cov_0.953309, whole genome shotgun sequence.
ACCESSION   DBMDYA010000549 DBMDYA010000000
VERSION     DBMDYA010000549.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_2050_cov_0.953309"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="ACZPFK_11760"
     CDS             complement(<1..1720)
                     /locus_tag="ACZPFK_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1407027.1"
                     /translation="MKLKFKHQPFQAEAAAAVCDVFNGQPLRTANYRIDLGDTSNLQQ
                     SMYQLTDVGFRNHPLVPELTRSRLLENLRAVQIRNNLKPSDSLAGPGINLTIEMETGT
                     GKTYTYVKTMYELNRRYGWSKFIIVVPSVAIREGVAKSLETTKQHFSDEYGKKIRFFT
                     YSSDKLTEVDNFASDSGIYAMIINMQAFNSSKNQKIIDKKLDSFRSRRPIDIIAQTNP
                     ILIIDEPQSVEGKQTKESLKKFNALFTLRYSATHKEKYDMVYRLDAMDAYNQHLVKKI
                     AALGITLTGSTATNGFVYVEGVDVYKNKAPTARLGFEVKGKTGTRTVVRKVQGGNDLY
                     TLSGELDEYADRFVILPDGIDGRDNSVTFLNGLKLYAGQISGNEQMTALQRRIQIRET
                     IRTHIQRERELYPRGIKVLSLFFIDEVSKYRLYDGDNDDGRNGEYAKMFEEEYANVVG
                     QMQRQFGDDAYMRYLDGIDVHKTHQGYFSIDKKKGKKVRFVEGKIDRKTQLSDDVDAY
                     DLIMKDKERLLSLDEPVRFIFSHSALREGWDNPNVFQICTLKPQSESEIRSRQEIGRG
                     LRLCVNQ"
     gene            complement(1720..>2050)
                     /locus_tag="ACZPFK_11765"
     CDS             complement(1720..>2050)
                     /locus_tag="ACZPFK_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIR1407028.1"
                     /translation="LMLGETVSNIKEDRTDMDLLYACLLDWGVEINLPHTSTQVAGCT
                     VHNVDNGALAACFDKAVPRAVVEYIANLQPLRAVFRDEAFATDAEKINVTEIFKNLSP
                     DTKVKVI"
BASE COUNT          392 a          559 c          586 g          513 t
ORIGIN      
        1 gctggttgac acacaggcgt aggccgcgac cgatctcctg gcggctgcgg atctcagact
       61 cgctctgagg tttcagtgtg caaatctgga acacgttggg attgtcccag ccttcgcgca
      121 gggccgagtg ggaaaagata aaccgcaccg gttcgtccag gctgagcagc cgttccttgt
      181 ccttcataat caggtcgtag gcatccacat catcggacag ctgcgttttg cggtcgatct
      241 ttccttccac aaagcggact tttttgccct tctttttgtc gatagaaaaa tacccctggt
      301 gggtcttgtg cacgtcaatg ccgtccagat aacgcatata ggcgtcatcg ccaaactgac
      361 gctgcatttg acccaccacg tttgcgtact cctcctcaaa cattttggcg tactcgccgt
      421 tgcggccatc gtcgttgtcg ccgtcatata ggcggtactt ggatacttcg tcgatgaaaa
      481 acagtgacag caccttgatg cccctgggat acaattcccg ctcccgctgg atatgggtac
      541 ggatggtctc tcggatctgg atccggcgct gcaaggctgt catctgttcg ttgccgctga
      601 tctgtccagc atagagtttg aggccgttga ggaaagtgac cgagttgtcc cgcccatcga
      661 tgccgtccgg caaaatcaca aagcggtcgg catactcgtc cagctcaccg gagagggtgt
      721 acaggtcgtt gccgccctgc accttgcgca ccacggttcg ggtgccggtc ttgcccttta
      781 cctcaaaacc caggcgggcc gtgggggcct tgttcttgta gacgtccacg ccctccacat
      841 agacgaaacc attggtggcg gtggagccgg tcaaggtaat gcccagcgcc gcgatctttt
      901 tgaccaggtg ctggttgtag gcgtccatcg cgtccagacg gtacaccatg tcatatttct
      961 ctttgtgggt ggctgaatag cgcagggtga acaaggcgtt gaactttttt aggctttctt
     1021 tggtctgttt gccctccacg ctctgcggtt catcgatgat caggatcggg ttggtctggg
     1081 caataatatc gatagggcgg cggctgcgga aactgtccag ctttttgtcg atgatcttct
     1141 ggtttttgct ggagttgaac gcctgcatat tgatgatcat ggcatagata ccgctgtccg
     1201 aggcaaaatt gtccacctcc gtcagcttat ccgatgaata ggtaaaaaac cggatcttct
     1261 tgccgtattc gtcggaaaaa tgctgtttgg ttgtttccag cgatttggcc acgccctcgc
     1321 ggatagccac cgagggcacc acgatgatga acttgctcca accgtagcgg cggttgagct
     1381 cgtacatggt tttgacgtag gtgtaggttt tgccggtgcc ggtctccatc tcaatggtca
     1441 ggttaatgcc ggggccagcc aggctatcgg agggtttcaa attattgcgg atctgcaccg
     1501 cgcgcaggtt ttccaaaagg cggctgcggg tcagctccgg caccagggga tgattgcgga
     1561 agcccacgtc ggtcagttgg tacatactct gctgcagatt gctcgtgtcg cccaggtcga
     1621 tgcggtaatt ggcagtgcgc agaggctggc cgttgaacac atcacagacc gcagctgcag
     1681 cctctgcctg gaagggctga tgtttgaatt tgagtttcat cagatcacct tcaccttcgt
     1741 gtcgggcgaa agatttttga agatctccgt cacgttgatc ttttcggcat cggtggcaaa
     1801 agcttcatcg cggaacactg cccgcagcgg ctgcaggttg gctatgtact cgaccacagc
     1861 gcggggcacc gccttgtcaa agcaggcggc cagggcgcca ttgtccacgt tatgtacggt
     1921 gcagcccgcc acctgggtgc tggtgtgggg caagttgatc tccacacccc agtccagcag
     1981 gcaggcatag agaaggtcca tatcggtgcg gtcctccttg atgttgctga ccgtttcgcc
     2041 cagcatcagc
//
LOCUS       DBMDYA010000550         2049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11303_length_2049_cov_1.095483, whole genome shotgun sequence.
ACCESSION   DBMDYA010000550 DBMDYA010000000
VERSION     DBMDYA010000550.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11303_length_2049_cov_1.095483"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="ACZPFK_11770"
     CDS             complement(<1..666)
                     /locus_tag="ACZPFK_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1407029.1"
                     /translation="MTHPLSQRAGLSATALKLIAVAAMTGDHLGWAFLPNASAAGVLV
                     HLLGRITMPLMCFFIAEGYRCTRSVPRYALRLFFCALASAIPYSYYQTGELFSNPFSV
                     LYTLLLGLLAIWACDRIPQPFLRAAVLLGLFLLSVPGDWGAYAVGWCLIFFLLRDRPG
                     PCAACFCALCLLHVLEAFSAGVSCGWGAAELFCATFVQSGCLLALPLLSFYDGRRGGE
                     RVHT"
     gene            1489..2001
                     /locus_tag="ACZPFK_11775"
     CDS             1489..2001
                     /locus_tag="ACZPFK_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1407030.1"
                     /translation="MLLSKPQRMARCAMIAALYTAVSLLLAPFSYGAVQVRVAEALTL
                     LPVFTPDAVVGVTLGCLLTNLLGSSPIDAVFGTLATLAAALLTYALRRVRWRSLPVAA
                     ALMPVLVNALVVGAEITVFFMDAPFSLPALAFNMLTVGLGEVVSCLGLGLLLVKCVEK
                     NRTLGEFLTA"
BASE COUNT          345 a          651 c          726 g          327 t
ORIGIN      
        1 tgtgtgcacc cgttcgccgc cgcggcggcc gtcgtagaaa gagagcagcg gcagcgccag
       61 caggcagccg ctctgcacaa aggtcgcaca gaacagctcc gccgcgcccc agccgcagga
      121 gacgcccgcc gaaaaggcct ccagcacatg cagcaggcac agcgcgcaaa aacaggctgc
      181 gcacgggccg ggccggtcgc gcagcagaaa aaagatcagg caccagccca ccgcgtaggc
      241 gccccagtcg ccgggcacag agagcagaaa caggcccagt agcaccgcgg cgcggagaaa
      301 gggctgcggg atgcggtcgc aggcccagat cgccagcagc cccaacagca gggtatagag
      361 cacgctgaac gggttggaaa agagctcgcc cgtctgataa taggagtagg ggatcgctga
      421 ggcaagcgca cagaaaaaca gccgcagcgc atagcggggc acgctgcggg tgcagcggta
      481 gccctcggcg ataaaaaagc acatcagcgg catggtgatg cgccccagca ggtgtaccag
      541 cacgcccgcg gcgctggcat tgggcaaaaa ggcccagccc agatggtcgc cggtcatggc
      601 ggcgacggcg atgagcttca gggccgtggc cgacaggccc gcccgttggg agaggggatg
      661 tgtcataggt gcacctcgac tttgcggggg gatggatgct tgaatcatca ttatacgcct
      721 cccgccccgg gatgaaaagg gggcactgtc ggtcgtgttg gggtggctgc cggccgctgg
      781 tgcggctgcc ggctgtgagg gcccgctgtt cgtccgctgg gacggttgcc ggtcgcgggg
      841 gtcgttgccg tcgacagact ggctgcaaaa aattcttttc cccgtccccg gggccgcttt
      901 tcctcggttt ttgggctgcg tgcgatttcc gccctgtgca tccggcgcgg tgcggccgaa
      961 gcgactttag ccggggagag cgggcagggg ggacaaaatg ggggcgtttt ttcaatttgt
     1021 cccgcgggta cggagaggac aagccccggg cgttctgcgc gtagacgccc ccagcccccg
     1081 aaagggcaag acccggaggg aaaaacgttc cgtccggagc ggcgacgcgg gccggatatt
     1141 tttggcccgg ccccctggcg aaaaacgggc ggatatggta caatgatgca cggccgactt
     1201 tggcggccgc agcggaaaag ggaaccctgc cctggggccg gtttggcccc gcttcctgcg
     1261 gcgcgcagtt tgcggccggg cgccctgcgg ggcgccgtca ccccggcggg aattctgcgg
     1321 cgccgccggg agaaaatctg ccgcggggcc tggggctgtt ttccccagag gcaaggacac
     1381 gtgaaacgac agggcccgcc tggggcggga cagggtgcag ctgcctgtcc cgcccggcgg
     1441 gcccgggtcc gggttgtcca tgccgctgcc cgaaaggagt tttgatccat gctcttgtcc
     1501 aaaccgcagc gcatggcgcg ctgcgccatg atcgccgccc tctacaccgc cgtcagcctg
     1561 ctgctggcgc ctttctctta cggcgccgtc caggtgcggg tggccgaggc cctcaccctg
     1621 ctgcccgtct tcacgccgga cgccgtggtg ggggtgacgc tgggctgcct gctcaccaac
     1681 ctgttgggct cgtcgcccat cgacgcggtc ttcggcaccc tggccaccct ggcggcggcc
     1741 ttgctcacct atgcgctgcg cagggttcgc tggcgctcgc tgccggtggc cgcggcgttg
     1801 atgccggtgc tggtgaacgc tctggtggtg ggcgccgaga tcaccgtctt tttcatggac
     1861 gcgccgttca gcctgcccgc gctggcgttc aatatgctca ccgtgggcct cggcgaggtc
     1921 gtcagctgtc tgggtctggg cctgctgctg gtcaaatgtg tggagaagaa ccgcaccttg
     1981 ggcgagtttc tcaccgcctg accggtttcg gcggccaata aaagaacaac agggtaaaaa
     2041 ggcccgccg
//
LOCUS       DBMDYA010000551         2039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11368_length_2039_cov_1.593395, whole genome shotgun sequence.
ACCESSION   DBMDYA010000551 DBMDYA010000000
VERSION     DBMDYA010000551.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11368_length_2039_cov_1.593395"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="ACZPFK_11780"
     CDS             <1..435
                     /locus_tag="ACZPFK_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407031.1"
                     /translation="GWMGGKVFGMPTLLIAHITFCVPYVIFNVSPKLPQMDVRMWEAA
                     LDLGCTPVQAFFKVVLPEIMPAVLSSFLICLTYSIDDFIISYFTSGTVQTLPIAIYSM
                     TRKKVSPEINALSTIIFVVILSIILIINAKDSRQAKKEAVRD"
     gene            489..1703
                     /locus_tag="ACZPFK_11785"
     CDS             489..1703
                     /locus_tag="ACZPFK_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1407032.1"
                     /translation="MKKVVSCILSVFLLALTLAAPAQAAVAVEDNGYDWERFQGQDIT
                     LNVYNWGLYISDGSDGGMDINKEFEELTGIKVNYTTYDTNESMYAKIKSGGADYDVVV
                     PSDYMIGKMIKEGLLAKLDFSNIPNMSLIGSQYKGRDYDPGDEYCVPYTWGVVGIIYN
                     TTMVEGEIDSWNALWDPAYKGNVLMFNNSRDAFAIASKKLGLSLNPSTAEEVQQAAEE
                     LKKQKSVVQAYVMDEIFDKMEGGEAALAPYYAGDAIVMMEENPDLAFAIPKEGTNYFV
                     DAMCVPATSAHKEAAEMYINFMCETEVALANCEFIGYSTPQVEAEQLLPDELKNSPIM
                     YPSQEVLANTETFNVLSDELNKAMDEAWSNVKSYDEGGSAWLIPTLLAVAVALIVIIL
                     WRKSVKKKRNDY"
BASE COUNT          471 a          556 c          609 g          403 t
ORIGIN      
        1 ggctggatgg gcggcaaggt gttcggcatg ccgacgctgc tcatcgccca catcaccttt
       61 tgcgtgccct atgtcatctt caatgtgtcg cccaagctgc cccagatgga cgtgcggatg
      121 tgggaggcgg cccttgacct gggctgcacg ccggtacagg cgtttttcaa ggtggtgctg
      181 cccgagatca tgccggcggt gctgtccagc tttttgatct gcctgaccta ttccatcgac
      241 gattttatca tcagctattt caccagcggc accgtgcaga cgctgcccat tgccatctac
      301 agcatgaccc gcaaaaaggt cagcccagag atcaacgcgc tgtccaccat catctttgtg
      361 gtgattttgt ccatcatcct gatcatcaac gccaaggaca gccgccaagc aaaaaaagaa
      421 gccgtgcgcg attgacgcgg ataaatcaaa aaaagagagt gtcgtgacaa cgagaggaga
      481 aacttgaaat gaaaaaagtg gtaagctgca tcttgtctgt ctttttgctg gcgctgaccc
      541 tggcggcgcc ggcgcaggcc gcagtggcgg ttgaggacaa cggttacgac tgggagcgct
      601 ttcaagggca ggatatcacc ctcaacgtct ataactgggg cctttatatc tcggacggct
      661 ccgacggcgg tatggatatc aacaaggagt ttgaagagct gaccggcatc aaggtgaact
      721 ataccaccta tgataccaac gagagcatgt acgccaagat caaatccggc ggcgcggact
      781 acgacgtggt ggtccccagc gactacatga tcggcaagat gatcaaggag gggctgctcg
      841 ccaagctgga tttttccaac attcccaaca tgtccctgat cggcagccag tacaagggac
      901 gggactacga cccgggcgat gaatactgcg tgccctacac ctggggagtg gtgggcatca
      961 tctacaacac cacgatggtg gagggggaga tcgacagctg gaacgccctg tgggaccccg
     1021 cctataaggg caatgtgctg atgttcaaca acagccgcga cgcctttgcc atcgcctcca
     1081 aaaagctggg cctgagcctg aatccctcca ctgcggagga ggtgcagcag gcggccgagg
     1141 agctgaaaaa gcaaaagagc gtcgtccagg cctatgtgat ggacgagatc ttcgataaga
     1201 tggagggcgg cgaggcggcg ctggctccgt actatgcggg cgacgccatc gtcatgatgg
     1261 aggaaaaccc cgatctggcg ttcgccatcc ccaaagaggg caccaactat tttgtggacg
     1321 ccatgtgcgt gcccgccacc agcgcccaca aagaagcggc cgagatgtat atcaatttca
     1381 tgtgcgagac cgaggtggcg ctggccaact gcgagtttat cggctattcc accccgcagg
     1441 tggaggcgga acagctgctg ccggacgagc tgaagaacag ccccatcatg tacccgtccc
     1501 aggaggtgct cgccaacacc gagacgttca acgtgctgtc cgacgagctg aacaaggcca
     1561 tggacgaggc gtggagcaac gtcaagagct atgacgaggg cggcagcgcc tggctcatcc
     1621 ccacgcttct cgcggtggcg gtggcgctga tcgtcatcat cctctggcgc aaaagcgtca
     1681 agaaaaaacg caacgattat tgagcggata aaaaaggggc ccggaaatta tttccgggct
     1741 cttttgtgtg ttcttcctgt gaaaaaggaa gcggcggcag gcgcggcggt ttacattttt
     1801 cgtcttttgg gataaaatga tagggtctga atttgacttt tttcggtcgc cgccgcccgg
     1861 cttgccggct gtgcccggtg cggcggcgaa cattttagat agaaacgggg cgatctcatg
     1921 ccacaggaat gcctgcggat ggagcatatc tgcaaggcct acaccgaggg ctttgtcacc
     1981 atcaaggacg ccagcctcac cctggctgcg ggggaaattc acggcctgct gggggagaa
//
LOCUS       DBMDYA010000552         2033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11403_length_2033_cov_1.251035, whole genome shotgun sequence.
ACCESSION   DBMDYA010000552 DBMDYA010000000
VERSION     DBMDYA010000552.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11403_length_2033_cov_1.251035"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..1231)
                     /locus_tag="ACZPFK_11790"
     CDS             complement(167..1231)
                     /locus_tag="ACZPFK_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1407033.1"
                     /translation="MMAATPKTKRLTDGELSVFCAQIAMLLRSGISVTEGLGIMVEDT
                     ENAEGRAILQTALDHCELGEALTAGLAASGAFPRYLLDMAAIGEETGRLDEVMDALAA
                     YYEREEALHEAVRSAVRYPLVMIVMMVVVLGVLLVKVLPVFNEVFLQLGAEMSGISLS
                     ILHVGQALSRYSVVLVVIAAAVAAFCLWCSKTAAGRAFKAKHFGGRVFGSRKLAAKIG
                     SARFASGMSLMLASGLDTDKSLAMVGRLVDNPQVQQKIVRCQKLIEEEGQNFGDALVG
                     SELFSGVYARMVTVGFKTGSADAVMKKLADRYQDEVDAEVTRLVSLVEPTLVAVFSVL
                     VGFILLSVMLPLMGIMSSIG"
     gene            complement(1276..>2033)
                     /locus_tag="ACZPFK_11795"
     CDS             complement(1276..>2033)
                     /locus_tag="ACZPFK_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1407034.1"
                     /translation="TIDCSNTADGYFMVNYAGGSPKVRMLVDGPDGVQYKFALNGGWE
                     TFPLSAGAGSYQINVYESAGGNEYYPLYGVTVNAPVKDEFATFLRPNQFVRYDAGTAA
                     VATAAELAQGAETDLEVVDRVYNYVLEHVDYDYGKAEVASRGSVQNFLPDVDETLASG
                     GGICFDYAAVMATMLRTQRIPTRLDIGWAGDIYHAWVSVYVDDVGWINGIIQFDGHEW
                     KRMDPTFADNGNQEEWIMDFIATDSNYNVLLVY"
BASE COUNT          352 a          718 c          630 g          333 t
ORIGIN      
        1 aaaaaaatca ccggcagggg ccgggccgca gcgcgggccc ggcgggggaa agcgcaggcg
       61 gcgggccgtg cggccccgcg cctgtctggg gcgctgcgcg cgccccggct ggagaaaaac
      121 ggcaaagcgt ctctttcggc tttggccgcg cggccgcgca gggcgctcag ccgatggagg
      181 acatgatgcc catcagcggc agcatcaccg acagcaggat aaagccgacc agcaccgaga
      241 acacggccac cagcgtgggc tccaccagcg agaccagcct tgtcacctcg gcgtccacct
      301 cgtcctggta gcggtcggcc agctttttca tcaccgcgtc ggcgctgccg gtcttaaagc
      361 ccaccgtcac catccgcgcg tagacgccgc tgaacagctc tgagcccacc agcgcgtcgc
      421 cgaagttctg gccctcctcc tcgatcagct tctggcagcg gacgattttc tgctgcacct
      481 gggggttatc caccaggcgg cccaccatgg ccaggctctt gtcggtgtcc agcccgctgg
      541 ccagcatcag cgacatgccg ctggcaaagc gggcgctgcc gatcttggcc gccagcttgc
      601 ggctgccgaa gacccggccg ccgaaatgct tcgctttaaa agcgcgcccc gccgcggttt
      661 ttgagcacca caggcagaaa gccgccacgg cggcggcgat caccaccagc accaccgagt
      721 agcgggacag cgcctgcccc acatgcagga tggagaggga gatgccgctc atctcggcgc
      781 ccagctgcag aaaaacctcg ttgaacaccg gcagcacctt gaccagcagc acgcccagca
      841 ccaccaccat catcacgatc atgaccagcg ggtagcgcac cgcgctgcgc acggcctcgt
      901 gcagggcctc ctcccgctcg taataggcgg ccagggcgtc catgacctcg tccagacggc
      961 cggtctcctc gccgatggcc gccatatcca gcagatagcg gggaaaagcc ccgctggccg
     1021 cgagccccgc cgtcagggcc tcgcccagct cgcagtggtc cagcgccgtc tgcaggatgg
     1081 cgcgcccctc cgcgttttca gtgtcctcca ccataatgcc cagcccctcg gtcaccgaga
     1141 tgcccgagcg cagcagcatg gcgatctggg cgcaaaagac cgacagctcg ccgtcggtga
     1201 gccgctttgt ctttggtgtt gccgccatca tatcctcctc ctgtccgcag gttgtgctcc
     1261 gtgcaaggcc gccggtcagt acaccagcag cacgttgtag ttgctgtccg tggcgataaa
     1321 atccatgatc cactcctcct ggttgccgtt gtcggcaaag gtggggtcca tccgcttcca
     1381 ctcgtggccg tcgaactgga tgatgccgtt gatccagccc acgtcgtcca catagacgct
     1441 gacccaggcg tggtagatgt ccccggccca gccgatgtcc agccgggtgg ggatgcgctg
     1501 ggtgcgcagc atggtggcca tcaccgccgc gtagtcaaag cagatgccgc cgccgctggc
     1561 cagcgtctcg tccacgtcgg gcaaaaagtt ctgcaccgac ccgcgggagg ccacctccgc
     1621 cttgccgtag tcgtagtcca cgtgctccag cacataattg taaacccggt ccaccacctc
     1681 gagatcggtc tcggcgccct gggccagctc ggccgcggtg gccaccgcgg cggtgcccgc
     1741 gtcgtagcgc acaaactggt tgggccgcaa aaaggtggcg aactcgtctt tcaccggcgc
     1801 gttgacggtg acgccgtaga gcgggtagta ctcgttgccg cccgcgctct cgtagacgtt
     1861 gatctgatag ctgcccgcgc cggctgacag cggaaaggtc tcccagccgc cgttgagggc
     1921 gaatttgtac tgcacgccgt ccgggccgtc caccagcatg cgcaccttgg ggctgccgcc
     1981 ggcgtagttg accataaaat agccgtcggc ggtgttggag cagtcgatgg tca
//
LOCUS       DBMDYA010000553         2021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11479_length_2021_cov_1.525521, whole genome shotgun sequence.
ACCESSION   DBMDYA010000553 DBMDYA010000000
VERSION     DBMDYA010000553.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11479_length_2021_cov_1.525521"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="ACZPFK_11800"
     CDS             <1..339
                     /locus_tag="ACZPFK_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407035.1"
                     /translation="RPQSRPARRCTARGSRKNVAAAEDNRNWVTETFVGMVDDRLQVD
                     TLASIEYMDGTATEYLNSAEDEALISKEKYDNRRNNILGSSALQEKIVLSKDRAVCQF
                     TDEEALKNIF"
     gene            complement(646..1599)
                     /gene="gluQRS"
                     /locus_tag="ACZPFK_11805"
     CDS             complement(646..1599)
                     /gene="gluQRS"
                     /locus_tag="ACZPFK_11805"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR1407036.1"
                     /translation="MEEKVAGRFAPSPSGRLHLGNLFCSLLAWLSAKSRGGRVVLRIE
                     DLDPARTSRAFADQLERDLEFLGLFWDEGGSGGGPHAPYYQSERAPLYEQCLAALEAR
                     GLVYPCFCSRAQLHAAEAPHAADGEPIYTGRCRALSPEEVRRRARTRSPALRLEVPPE
                     AVSLTDGHYGFFSQNLARDCGDFILRRSDGVFAYQLAVVADDAAMGVTEVVRGRDLLA
                     SAPRQIYLYRLLGAPPPSFCHAPLLLASDGRRLSKRDADLGLDALRARGFSAADITGR
                     LAFAAGLLDSPQPASPQELLPLFSWNKVPTQDIPLPAGLFD"
BASE COUNT          465 a          559 c          611 g          386 t
ORIGIN      
        1 cggccacaga gccgtcccgc ccggcgctgt acggcccgcg ggagcaggaa aaatgttgcg
       61 gctgctgagg acaatagaaa ttgggtcacg gaaacatttg tgggcatggt agacgaccgg
      121 ttacaggttg atacattggc cagtatagaa tatatggacg ggacagcaac agaatattta
      181 aattctgcag aagacgaagc acttatttca aaagaaaaat acgataatag acgcaataac
      241 attttaggta gctctgcttt gcaagaaaag atcgttttat caaaagacag agccgtctgt
      301 caatttacag atgaagaagc cctaaaaaat atattttgaa gctgcttaac gaatcttctt
      361 ttgcaaaaga aagcaaataa gtatttttaa aaggtgatat agaaattttt gttttcttcc
      421 gtcaagactt tatatttttt tctatcgttt atgtcagaaa ttcttttcat ctgcatattc
      481 tgtaaggttt ttaagacatt ttcacgaaga ataagaagac ttttatcttg atgactgcga
      541 tccgagcccg ggccgtatgc gcgtcgcacc ctacagcctt aaaattttga gagcccgccc
      601 acagattgga ccgaagcacg gaataaaact tgccgaaaac acacttcaat cgaacagccc
      661 ggcgggcagg gggatatcct gggtcgggac cttgttccag gaaaaaaggg gcaacagctc
      721 ctgaggggag gcgggctggg ggctgtccaa caagccggcg gcaaaggcca ggcggccggt
      781 gatatccgcg gcggaaaatc cccgcgcccg cagggcgtcc agccccagat ctgcgtccct
      841 tttggacagg cgacggccgt ccgacgccag cagcagcggc gcgtggcaga aagacggcgg
      901 cggcgcgccc agcagccggt agagataaat ctggcgcggc gcagaggcga gcaggtcccg
      961 gccgcgcacc acctcggtca cccccatggc ggcatcgtcc gccaccactg ccagttgata
     1021 ggcaaagacg ccgtccgacc ggcgcaggat gaagtcgccg cagtcccgcg ccaggttttg
     1081 agagaaaaag ccgtagtggc cgtccgtcag gctgacggcc tccggcggca cctccagccg
     1141 cagcgccggg gaacgggtgc gggcgcggcg gcgcacctcc tcgggggaga gggcgcggca
     1201 gcgaccggta tagataggct cgccgtcggc ggcatgaggg gcctcggcgg cgtggagctg
     1261 ggcacggctg caaaaacagg gatagaccag gccgcgcgcc tccagcgcgg cgagacattg
     1321 ttcgtacagg ggggctcgct cgctctggta gtagggcgcg tgcgggccgc cgccgctgcc
     1381 gccctcgtcc caaaagaggc cgagaaactc aaggtcccgt tccagctggt cggcaaaggc
     1441 gcggctcgtg cgcgccgggt ccagatcctc gatgcgcagc accactctgc cgccccggct
     1501 tttggcggag agccacgcca acagggaaca gaacaggttg cccagatgca gccggccgct
     1561 ggggctgggg gcaaaacggc ccgccacttt ttcttccatc cgctcacctc cgtggaacag
     1621 ggtatttatc tatgatacct attataatag ggcggcagtg gtcctttcaa cgcttttggc
     1681 aaagcagctg gtaaaaactg ctttcaaaaa tcagcggacc ggggacgcag ggggtatttg
     1741 gcccggatta tagcggcgcg gggaggaaag aggatcttct cgggtgcaga aaaggcaaaa
     1801 gggacgatac gcaaggtatc gtccctttcc aatttgcccg gatggcccgt tttcggcgct
     1861 gtacggcggc cgaggcaggc ggataaaaac ggcctgcgct ttgccgcagc ggcctgttgc
     1921 tgttcgctcc cgcgcctccc gatctttcgg gcacagcttc gccgcacctg tttcaccgct
     1981 tccattcccc gcgctgtcgc ggcaaccgaa acggatggtc a
//
LOCUS       DBMDYA010000554         2017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11495_length_2017_cov_1.570459, whole genome shotgun sequence.
ACCESSION   DBMDYA010000554 DBMDYA010000000
VERSION     DBMDYA010000554.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11495_length_2017_cov_1.570459"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            321..590
                     /locus_tag="ACZPFK_11810"
     CDS             321..590
                     /locus_tag="ACZPFK_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024426.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HIR1407037.1"
                     /translation="MENLNAKDYDAVVAGMSDEMKEALPADKLAEVWAPVAEQLGAFD
                     SFSKDSTAEKDGLAVVIVTAKYENAGLTYTLTFDTELRLCGLYMK"
     regulatory      658..765
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            842..1420
                     /gene="thiT"
                     /locus_tag="ACZPFK_11815"
     CDS             842..1420
                     /gene="thiT"
                     /locus_tag="ACZPFK_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR1407038.1"
                     /translation="MTKTYSKTRTLVECALMIAIGTVLAQIKVFEMPNGGSVTLLSML
                     PFVLVSFRHGVKWGLFTGFVNSLLQMLLGFYAPPSNTVWALVGMIMLDYVLAFSCLGL
                     ACVFAKPFKNKLLGVAVGTAAVCFLRFLCSYMSTVFVWFSTAPAGMPVWLFAITYNGA
                     YMLPELILTTAGAVLLYRFAPVLFSSVGAKKA"
     gene            1768..>2017
                     /locus_tag="ACZPFK_11820"
     CDS             1768..>2017
                     /locus_tag="ACZPFK_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR1407039.1"
                     /translation="MFETRPAQEVLSDLGSGVTGLSESEAARRLASDGENALKEKEPP
                     TRLQLFLAQLKDPMIYVLLAAAVISVALGEFSDAVIILG"
BASE COUNT          378 a          589 c          577 g          473 t
ORIGIN      
        1 acacttacaa ggctcagcaa aaggcggcgg caaaggccgc tgcgaaagcg gcggaatgac
       61 gggtttgcct ttccagccgc cgagagggag ggtttcgtcc tcttttctct cgacggcccc
      121 gtcctttatc cggcccggca ttttgaaagc ggttttgacg gcacaaaagc cgggatgtgc
      181 aaaaaaggag gaccacctca tgaaaaaact gttttgcctg ctgctctgtt ccctgctctg
      241 cgccgggctg ctcgccggct gcggggggcc tgtcggacaa ctacgatgaa gaggaggtcg
      301 tctccctcgc ccacgcggtg gtggagaact tgaacgcaaa agactacgac gccgtcgtcg
      361 cgggcatgag cgacgagatg aaagaggcgc tgcccgccga caagctggcg gaggtgtggg
      421 cgcccgttgc ggaacagctg ggcgctttcg actcgttctc caaggacagc accgcagaaa
      481 aagacggcct tgctgtggtc atcgtgaccg cgaaatacga aaacgccggt ctcacctata
      541 ccctcacttt cgacaccgag ctgcggctgt gcgggctcta tatgaaatag acggcggccc
      601 ggactatttt ttcgattttt cttgacaagc cgctcccgcc tgctataatg aggacggaca
      661 taaaaacagg ggtgccgaaa ggctgagatt tgcgtcgtgc gcggcgcatg aaccctttaa
      721 cctgatccgg ataatgccgg cgtaggaagt ttacagatca tcttcaaggt taaacacctg
      781 tttttattgt aaaaacaggt gtttttgttt ttatgtacta cttttttgtg aggttggtaa
      841 gatgaccaag acctattcca agacccgcac cctcgtcgag tgcgcgctga tgatcgccat
      901 cggcaccgtg cttgcccaga tcaaggtgtt cgagatgccc aacggcggca gcgtgaccct
      961 gctctccatg ctgcctttcg tgctcgtctc gttccgccac ggcgtcaagt ggggcctgtt
     1021 caccggcttt gtgaacagcc ttttgcagat gctgctgggc ttttacgcgc cgccctccaa
     1081 caccgtctgg gcgctggtgg gcatgattat gctggactat gtattggcat tctcctgcct
     1141 ggggctggcc tgcgtgttcg ccaagccgtt caaaaacaag ctgctgggcg tcgccgtggg
     1201 caccgccgcg gtctgctttc tgcggtttct gtgcagctat atgagcaccg tcttcgtctg
     1261 gttctccacc gcgccggcgg gcatgccggt ctggctgttc gccatcacct acaacggcgc
     1321 ctatatgctg cccgagctga tcctcaccac ggcgggcgcg gtgctgctgt accgcttcgc
     1381 ccccgtgctc ttctcttcgg tgggcgccaa aaaggcctga gctccaccgg acaatccggc
     1441 ggctttgtcg cacttttggc agtttcttcc tctttttgtc ttgacaacgc ctttttgcgg
     1501 ctttaatatt aaaggtacaa aagggagtag tttcaatccc cgcttccaac agccgccggt
     1561 tcttccggct ggggcggaga ccgcttgagg taacgagact tttgacgcgg cgtcacaggg
     1621 ccgtgtcaaa agtctttttt tgtcgcctct catccgcgcc gtgtgggatc gcgcaggcac
     1681 agggcgttat ccgccgcttt ttagcccata ctcaggaaag cacagttttc ccttttgatt
     1741 ttgcaacagt ttactggagg tatcgcgatg ttcgaaacga ggcccgccca agaggttctc
     1801 tcagacctgg gctccggcgt caccggcctg tcggagagcg aagccgcccg tcggcttgcc
     1861 agcgacggcg aaaacgcatt gaaagaaaaa gagcccccca cccgcttgca gctgtttctg
     1921 gcccagctca aagacccgat gatctacgtg ctgctcgccg ccgccgtgat ctcggtggcc
     1981 ctgggcgaat tttcagacgc ggtcatcatt ttgggcg
//
LOCUS       DBMDYA010000555         2015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11515_length_2015_cov_1.032915, whole genome shotgun sequence.
ACCESSION   DBMDYA010000555 DBMDYA010000000
VERSION     DBMDYA010000555.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11515_length_2015_cov_1.032915"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="ACZPFK_11825"
     CDS             <1..501
                     /locus_tag="ACZPFK_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407040.1"
                     /translation="DCLATPLPLETVTEEGEVSIRGRVIAHLICCNSLGELECYDKPC
                     EYVLPKRYTAGSGGVTVDVNALFEGARAQKNGGEASCEVTLSVSGTVMERQKLEVLDE
                     ITCEEPLPPDEDEIALRIYYGRAGEQLFEIAKYYHADPEQIAQQSGVEGDVLQENTRL
                     LIPAAE"
     gene            519..1988
                     /gene="spoIVA"
                     /locus_tag="ACZPFK_11830"
     CDS             519..1988
                     /gene="spoIVA"
                     /locus_tag="ACZPFK_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1407041.1"
                     /translation="MDYSIYKELIERTGGDIYIGVVGPVRTGKSTFIKKFMETLVLPA
                     IQNENVKLRAVDELPQSAAGRTIMTTEPKFIPEQAVNVELEEGISFRTRLIDCVGYMV
                     SGAMGSVEGEKPRMVKSPWFDHAVPFDVAAETGTKKVICEHSTIGLVITTDGSISDIP
                     REAYEASEQRVIDEMKATGKPFTVLLNCVSPASTASVALAKQLEERYGVGVLAVSCVD
                     LDEAAICEILKSVLYEFPIREIGFSMPKWVTMLGPEHWLQQDVYAAVQQFAAGVDRVR
                     DLRREQQIESEFVARSGVAKVDLGTGVARVEVTLQPDIFYRVLGETTGLEICDEASLM
                     PCIINLARIKKEYEKIKDAMDQVEATGYGIVMPTIDELHLEEPEIVKQSGRYGVKLRA
                     SAPSIQMLKVNITTEINPIVGSEKQSEELVHSMLDEFEEDPIRIWESNIFGKSLHELV
                     NDGLQGKLLHMPSEARMKLQETMERVINEGCQGLICIIL"
BASE COUNT          425 a          585 c          687 g          318 t
ORIGIN      
        1 gactgcctgg ccaccccgct gccgctggag accgtcaccg aggaggggga ggtcagcatc
       61 cgcgggcggg tcatcgccca tctcatctgc tgcaacagcc tgggcgagct ggagtgctac
      121 gacaagccct gcgagtatgt tctgcccaag agatataccg ccggcagcgg cggcgtcacc
      181 gtggatgtga acgccctttt tgagggggcg cgggcccaga aaaacggcgg cgaggccagc
      241 tgcgaggtca ctctctcggt gagcggcacc gtgatggagc gccaaaagct ggaggtgctc
      301 gacgagatca cctgcgagga gccgctgccg ccggacgagg atgagatcgc gctgcgcatc
      361 tactacggcc gcgcgggcga gcagctgttc gagatcgcaa agtactacca cgcggacccg
      421 gagcagatcg cacagcagag cggcgtcgag ggcgacgtgc tgcaggagaa cacccggctg
      481 ctgatcccgg cggcggagtg aggggaggtg agcatcagat ggattacagc atttataaag
      541 aactgatcga gcgcacgggc ggggacatct atatcggggt ggtcggcccg gtgcgcaccg
      601 gcaagagtac ttttatcaaa aaatttatgg agacgctggt gttgccggcg atccagaatg
      661 agaacgtcaa gctgcgggcg gtggatgaac tgccgcagag cgcggcgggc cgcaccatca
      721 tgaccaccga gccgaaattc atccccgagc aggcggtcaa tgtggagctc gaggaaggca
      781 tcagcttccg cacccggctc atcgactgcg tggggtatat ggtcagcggc gcgatgggca
      841 gtgtggaggg cgaaaagccg cgcatggtga agagcccctg gttcgaccac gccgtcccct
      901 ttgacgtggc cgcggagacc ggcaccaaaa aggtcatctg cgagcactcc accatcgggc
      961 tggtcatcac caccgacggc agcatcagcg acatcccccg cgaggcctat gaggccagcg
     1021 agcagcgggt catcgacgag atgaaagcca ccggcaagcc ctttaccgtg ctgctcaact
     1081 gtgtgtcgcc ggcctctacg gcaagcgtgg ccctggcaaa gcagctggag gagcggtacg
     1141 gcgtcggcgt gctggcggtg agctgcgtcg atctggacga ggcggcaatc tgcgagatcc
     1201 tcaagagcgt gctctatgag ttccccatcc gcgagatcgg cttctcaatg cccaagtggg
     1261 tgaccatgct ggggccggag cactggctgc agcaggacgt ctacgcggcg gtgcagcagt
     1321 tcgcggcagg ggtcgaccgg gtgcgcgacc tgcggcgcga gcagcagatc gagagcgagt
     1381 tcgtggcgcg ctcgggcgtg gcaaaggtcg atctgggcac cggtgtggcg cgggtcgagg
     1441 tcaccctgca gccggatatc ttttaccggg tgctgggcga gaccacaggc ctggagatct
     1501 gcgacgaggc cagcctgatg ccctgcatca tcaacctggc gcgcatcaaa aaagagtatg
     1561 aaaagatcaa ggatgccatg gaccaggtgg aggccaccgg ctacggcatc gtgatgccca
     1621 ccatcgacga gctgcacttg gaggagccgg agatcgtcaa gcagtccggc cgttacggcg
     1681 tgaagctgcg ggccagcgcg ccctccatcc agatgctcaa ggtcaacatc accaccgaga
     1741 tcaaccccat cgtgggctct gagaagcaga gcgaagagct ggtgcacagc atgctggatg
     1801 aatttgagga ggaccccatc cgcatctggg agtccaacat cttcggcaag tccctgcacg
     1861 agctggtcaa cgacgggctg cagggcaagc tgctgcacat gcccagcgag gcgcgcatga
     1921 agctgcagga gaccatggag cgggtcatca acgagggctg ccagggcctg atctgcatca
     1981 tcctttgacg ggtatttatt taaaacaaaa ccagg
//
LOCUS       DBMDYA010000556         1997 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11626_length_1997_cov_1.519515, whole genome shotgun sequence.
ACCESSION   DBMDYA010000556 DBMDYA010000000
VERSION     DBMDYA010000556.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11626_length_1997_cov_1.519515"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="ACZPFK_11835"
     CDS             <1..763
                     /locus_tag="ACZPFK_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR1407042.1"
                     /translation="LMEKGIIARKLKEYRQEAELTQQEVAAKIGRPQSTIAGWESARS
                     QPDADTLYSLLVLYDVSPNTFFGYDITSMDVSSEERELLCMFRKLSDFGKRLVRLVAE
                     EVAGEEESHSKPVPVSSQLSAPEATTSCLYLQLSEQPASAGTGVYLGPEAFRPIKVVA
                     NDKTCSAAFCVRVSGDSMEPIYRDGDIVMVSKEQPRNDDICLVTFDGCGYIKRIAPMK
                     LISENKKYPPIPLNNDVIVNGRVIGKLWPGWILEM"
     gene            complement(1325..>1997)
                     /locus_tag="ACZPFK_11840"
     CDS             complement(1325..>1997)
                     /locus_tag="ACZPFK_11840"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1407043.1"
                     /translation="QVRNTLGYPHLNVKIGATHGGISVGEDGASHQCCEDFALMRTIP
                     GMVVVSPSDDVEARAAVKAAYEHDGPVYLRFGRLAVPVINDGPDYKFELGKGVTLRDG
                     KDITVIATGLMVAEALAAAETLAAEGIDVRVLNIHTIKPLDEELVLKAARETGKIVTA
                     EEHNIIGGLGEAVCSCLAENCPTPVRRIGVNDEFGHSGPAAALLKQFGLSAEHIAEVV
                     RDMVK"
BASE COUNT          420 a          527 c          543 g          507 t
ORIGIN      
        1 tttaatggaa aagggaataa ttgcaagaaa gctaaaagaa taccgccaag aagctgaact
       61 tactcaacag gaggttgcgg ctaaaattgg ccgcccccaa tcaacaattg ctgggtggga
      121 aagcgcgagg tctcaacccg acgcagatac tttgtattca ctcctggttc tctatgacgt
      181 atcacctaat acgttcttcg gctatgacat cacttcaatg gatgtttcgt ctgaagaacg
      241 tgaattatta tgtatgttcc gaaagctcag cgattttggg aaacgcttag ttcgtctcgt
      301 tgctgaggaa gtggctgggg aagaggagtc tcattctaaa cctgtacctg tttcgtctca
      361 attatctgcg ccggaagcga cgacaagttg cctatattta caactttcag agcagcccgc
      421 cagcgctggc acgggggttt atttggggcc agaggctttc cgtccgatca aagtggtggc
      481 aaatgataaa acctgtagtg ctgcattctg cgtgcgtgtg agtggggaca gcatggagcc
      541 gatctaccgg gacggggata ttgtcatggt ttccaaagag cagcctcgca atgatgatat
      601 ctgtctcgta acctttgatg gatgtggtta tattaagcgg atcgccccaa tgaaactaat
      661 ctccgaaaat aaaaaatatc cgcccattcc tttgaacaat gatgtgatcg tcaatgggcg
      721 agtgattgga aaactatggc cgggctggat actggaaatg taacaaaagt tcatatttta
      781 agtgtgcatt taatttttaa taattattct tattatcctg ataaagccgc atcacacctt
      841 atgtgtgcac aaaattttta agtgtgcaga tttgcacact taaaacaaaa agaaccgttt
      901 gcagagagag cctcgtacgc tcaatccgta aacggttctt ttttatgtgt ttataagccg
      961 taaaaacggc ggtttcatgc ggttttgtgc tgctttttag tctccttgtt tacgccccgt
     1021 aaacggtttg taaacgcctg ttttcatccc tctggccatc gacctgtcac ccggcaaaac
     1081 gcctcaaaat gcccgaaatt cagttgcaag ttccgtttca gcattcgtca ttttccgccg
     1141 ccgtcttgcg acccgccgtc accctaaaac cgctttgcgt ggccgttttt caggtttttg
     1201 cgggttcttg cacctcaaac tctaggccgt tcgtggtttt ctcattcttc gtgcgttttc
     1261 acattcatcc catgggatga agtctctttt ttcggttttt caataaagct ctgttcgagc
     1321 agccttattt taccatgtcg cgcacgacct cggcgatgtg ctccgccgag aggccgaact
     1381 gcttgagcag cgcggcggcc gggccggagt ggccgaactc gtcgttgacg ccgatgcggc
     1441 gcaccggggt ggggcagttc tcggccaggc agctgcagac cgcctcgccc aggccgccga
     1501 tgatgttgtg ctcctcggcg gtgacgatct tgccggtctc ccgcgccgcc ttgagcacca
     1561 gctcctcgtc cagcggcttg atggtgtgga tgttcagcac ccgcacgtcg atgccctcgg
     1621 cggccagggt ctcggccgcg gccagcgcct cggcgaccat caggccggtg gcgatgacgg
     1681 tgatgtcctt gccgtcccgc agggtcacgc ccttgcccag ctcaaacttg taatccgggc
     1741 cgtcgttgat gaccggcacc gccaggcggc cgaaacgcag gtaaacgggg ccgtcgtgct
     1801 cataggcggc cttgaccgcg gcgcgcgcct ccacgtcgtc ggaggggctg accacgacca
     1861 tgccggggat ggtgcgcatg agggcgaagt cctcgcagca ctggtgggac gcgccgtcct
     1921 cgcccacgct gatgccgccg tgggtggcgc cgatcttgac gttgaggtgg gggtagccca
     1981 gggtgttgcg cacctgc
//
LOCUS       DBMDYA010000557         1996 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11631_length_1996_cov_1.774670, whole genome shotgun sequence.
ACCESSION   DBMDYA010000557 DBMDYA010000000
VERSION     DBMDYA010000557.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11631_length_1996_cov_1.774670"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="ACZPFK_11845"
     CDS             complement(<1..439)
                     /locus_tag="ACZPFK_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1407044.1"
                     /translation="MEKQLLSPPADLPRVEKQFRRGQIILEPTRENSFFHILIAGEAD
                     VCYQSENGEVLSMYTYRAADFFGEIELLSDRRQPLLVRARSDCRVLLHRDALLEWLHR
                     DFAFTRAVLKRLCEKLLEGSQARARLTLLPIRERYLLAVRRHRD"
     gene            659..1612
                     /locus_tag="ACZPFK_11850"
     CDS             659..1612
                     /locus_tag="ACZPFK_11850"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1407045.1"
                     /translation="MRKLIIDTDTGSDDAVALLMALRSPEVEVLGITTVGGNVPLEQA
                     TQNALMTVEVCGSRVPVYKGAAKPLFRELVTAVNVHGRDGMGDLDLIHPQSAAQATHA
                     VDYLIETVRANPGEVEIVTIGPATNLALALLKEPQVMRRTKHIWSMGTAGFGPGNTTP
                     VAEFNVYVDAESYAVLLNGGVPLTIVGIDQCKGNAVWTAQDMEEIRAGSRVGCFAVDC
                     NSVLRAFQKRRCGQDIVDLPDPVTMAVALWDDVALDKARCHCYCCTREEEAYGQVILH
                     EEGRVYAIDRPERPVNAEVVRRIDVPLYKARLKELLIGAEA"
BASE COUNT          354 a          641 c          674 g          327 t
ORIGIN      
        1 tgtcccggtg gcggcgcacg gcgagcaggt agcgctcgcg gatgggcagc agggtcagcc
       61 gcgcccgcgc ctgtgagccc tccagcagct tttcgcacag ccgcttgagc acggcgcggg
      121 tgaaagcgaa atcccgatgc agccattcca gcagggcgtc ccggtgcagc agcacccggc
      181 agtcgctgcg ggcccgcacc agcagcggct gccgccggtc ggacagcagt tcgatctcgc
      241 cgaaaaaatc cgccgcccgg taggtgtaca tggacagcac ctcgccgttt tcgctctggt
      301 agcagacgtc cgcctcaccg gcgatgagga tgtggaaaaa actgttttcc cgcgtcggct
      361 ccaggatgat ctgcccgcgg cggaactgct tttccacccg gggcaggtcg gcgggtgggg
      421 ataagagctg cttttccatc ccgatccctc cttttgccgg tcggcggcgc gccgtgccgg
      481 ccgctttcaa aaaaacagcg atccctgtcg gctcacgctt tgcccgcccc ggcgtttttg
      541 gtcatatgac ctgttttttt gattatacca cctttagaat gaaagaaaaa gcgagggaac
      601 aaggtctgcg gctgcacgga cggccgcctt ttcccccccc aaaaaggagt ggtctcacat
      661 gagaaaattg atcatcgaca ccgacaccgg cagcgacgac gcggtcgcgc tgctgatggc
      721 cctgcgcagc ccggaggtgg aggtgctggg catcaccacc gtcggcggca acgtgccgct
      781 ggagcaggcg acccagaacg ccctgatgac cgtcgaggtc tgcggcagcc gggtgccggt
      841 ctacaagggg gcggcaaagc cgctcttccg cgagctggtg acggcggtca acgtgcacgg
      901 ccgggacggc atgggcgacc tggacctgat ccatccccag agcgcggcgc aggccaccca
      961 cgcggtggac tatctgatcg agacggtgcg cgcgaacccg ggcgaggtgg agatcgtcac
     1021 catcggcccg gccaccaacc tggccctggc cctgctcaag gagccgcagg tcatgcggcg
     1081 gacaaagcat atctggtcga tgggcaccgc cggcttcggc cccggcaaca ccaccccggt
     1141 ggcggagttc aacgtctatg tggacgccga gagctatgcg gtgctgctga acggcggcgt
     1201 ccccctcacg atcgtgggca tcgaccagtg caagggtaac gcggtgtgga cggcgcagga
     1261 catggaggag atccgggccg gcagccgggt ggggtgcttt gcggtggact gcaacagcgt
     1321 gctgcgcgcg tttcaaaagc ggcgctgcgg ccaggatatc gtggacctgc cggaccctgt
     1381 caccatggcc gttgcgctgt gggacgacgt ggcgctggac aaggcccgct gccactgcta
     1441 ctgctgcacg cgggaggagg aggcctacgg gcaggtcatc ctgcacgagg agggcagggt
     1501 gtacgccatc gaccgcccgg aacgccccgt caacgccgag gtggtgcgcc gcatcgacgt
     1561 acccctttac aaggcgcggc tcaaagagct gctcatcggc gcggaggcat aatgcggggc
     1621 tgcccgcctt gagaaagggc ccccttgccc cggcgaccgg cgacggtctt aaaaaacgtt
     1681 gccccgagag gcggctctgc gcgcaggaca ccgcccaatt caccccgcag ccacaggagg
     1741 ggggaggggc ggcttcgccc ggcggactgc ctgcgcgggc aggcggccga cgtgaatttt
     1801 ccgggcagtc catcgcggct ttccctcccg gggacgcgcg gcggttttaa tccggcggtt
     1861 ttcaaggtga acacagtgca gaaaagggtt ggcaatccct gtggattgcc aacccttttt
     1921 gcatgtgccg ggcgtcaaag cggtcggtcc gaaaccaaag cttttttgcc tcaacgcgcc
     1981 cgaaaggcgc ctcttt
//
LOCUS       DBMDYA010000558         1995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11640_length_1995_cov_0.920803, whole genome shotgun sequence.
ACCESSION   DBMDYA010000558 DBMDYA010000000
VERSION     DBMDYA010000558.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11640_length_1995_cov_0.920803"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="ACZPFK_11855"
     CDS             complement(<1..681)
                     /locus_tag="ACZPFK_11855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1407046.1"
                     /translation="MYKKVLLIYNPVAGKATVPGKLSTIIESFSRAGSEVTVHPILPG
                     NGAEEVLAARGDAYDLVVCCGGDGTLNHTVNGLMRMKRPPLFGYLPSGSTNDFARSLE
                     LPQKIEDGCAALLEGSPFFYDIGSFNDRHFNYVAAFGAFTDVSYLTPQNDKNALGHLA
                     YLIEGVKRLPIGQHFPARVECGGTYCRGMIVTDLREHPFQAEYVRFAVEVDSGRAVRE
                     LMSVFWESQ"
     gene            complement(886..1305)
                     /locus_tag="ACZPFK_11860"
                     /pseudo
     CDS             complement(886..1305)
                     /locus_tag="ACZPFK_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558447.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            1330..>1995
                     /locus_tag="ACZPFK_11865"
     CDS             1330..>1995
                     /locus_tag="ACZPFK_11865"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1407047.1"
                     /translation="MSHGLSGVEALSGIPGTVGASPVQNVGAYGHEVAETISSVEAYD
                     RLTGTVVRLLPGDLGFAYRSSAIKRSVGEAGLNGRPWGPTGRWVVLSVDFRLERSPLS
                     APVMYSELARRLGVEAGERADASLVRSTVLELRRGKGMVLDPGDHDTWSAGSFFTNPI
                     LPEAAAASLPEGAPRFGAGEGLVKTSAAWLIDHAGCGKGFHLPEAGDPPRASLSTKHV
                     LALT"
BASE COUNT          323 a          648 c          660 g          364 t
ORIGIN      
        1 ctgtgactcc caaaacacgg acataagctc cctgacagcc cttccgctgt ccacctccac
       61 tgcaaagcgc acatactctg cctggaaagg atgttccctc aggtccgtca caatcatgcc
      121 gcggcagtag gtgccgccgc actccaccct ggccgggaag tgctgcccga taggcaggcg
      181 cttgaccccc tcgatcagat aggccagatg gcccagggcg tttttgtcgt tttgcggggt
      241 cagataggag acgtcggtaa aggcgccgaa agcggccaca tagttgaagt gccggtcgtt
      301 gaaggacccg atgtcgtaaa agaacgggct gccctccagg agggcggcgc agccgtcctc
      361 tattttttgc ggcagctcca gcgagcgcgc aaaatcgttg gtggagccgc tgggcagata
      421 gccgaacagg ggcgggcgct tcatccgcat caggccgttc acggtgtggt tgagagtgcc
      481 gtcgccgccg cagcagacca cgaggtcgta ggcgtccccg cgcgcggcca gcacctcctc
      541 ggcgccgttg cctggcagga tgggatgaac cgtcacctcg ctgcccgcgc gggaaaagct
      601 ctcgatgatg gtggagagct tgcccggcac cgtggccttg cccgccaccg ggttatagat
      661 gagcaacact tttttgtaca tgagaccgcc ccttgcctgt gtcttggaat agagcgggcg
      721 ccgctgaaaa gcgcccgctt tcgctgccag tatatcatct aattatgaac aaaaatggac
      781 gggaaaatct cacagccgta aacaaacgcc gcgccgcggg cagacaaaag ccccgcaggg
      841 cattcagctc tgcggggcca aagacagttg cgttggacgg ggcgattaca gctcggcgaa
      901 gtacttgatg gtgcgcacca tctggctggt gtagctgttc tcgttgtcgt accaggacac
      961 gacctgcacc tgataggtgt cgtcgtcgat cttggtgacc atggtctggg tggaatcgaa
     1021 caggctgccg tagcgcatgc cgatgacgtc ggaggagacg atctcctcct cgttgtagcc
     1081 gaagctctcg gaggcggcgg ctttcatggc ggcgttgatg gactctttgc tgacgtcctt
     1141 gcccttgacg acggccacga ggatggtggt ggagccggtg ggcacgggca cgcgctgggc
     1201 agagccgatc agcttgccgt tcagctcggg gatgaccagg ccgatggcct tggcggcgcc
     1261 ggtgctgttg ggcacgatgt tctgcgcgcc ggcgcgggca cggtctggga ccagttcgtc
     1321 tcctggacgc tgtcgcacgg gctgtccggc gtcgaggccc tttccggcat ccccgggacc
     1381 gtcggcgcgt cccccgtgca gaacgtgggc gcctacggcc acgaggtcgc cgaaaccatc
     1441 tcctcggtag aggcctacga ccgcctcacc ggaaccgtcg tgcgcctgct gcccggcgac
     1501 ctcggattcg cctaccgctc ctcggcgatc aagcgcagcg tcggcgaggc gggcctgaat
     1561 ggccgcccgt ggggcccgac cggccgctgg gtggtcctat ccgtggactt ccgtttggag
     1621 cgctcgcccc tgagtgcccc cgtcatgtac tccgagctcg cccggcgcct cggcgtcgag
     1681 gccggggagc gcgccgacgc gtccctggtg cgttcgaccg tcctggagct gcgccgcggc
     1741 aagggcatgg tcctcgaccc gggtgatcac gatacgtgga gcgccggttc cttcttcacg
     1801 aacccgatcc tgcccgaggc cgctgccgcc tccctgcccg agggcgcgcc tcgctttggt
     1861 gcgggggagg gcctcgtgaa gaccagcgct gcctggctca tcgaccacgc gggctgtggt
     1921 aagggcttcc acctgcccga ggccggggac cctccgcgcg cgtccctgtc caccaagcac
     1981 gtgctggccc tgacg
//
LOCUS       DBMDYA010000559         1984 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11688_length_1984_cov_1.627191, whole genome shotgun sequence.
ACCESSION   DBMDYA010000559 DBMDYA010000000
VERSION     DBMDYA010000559.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_1984_cov_1.627191"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="thyX"
                     /locus_tag="ACZPFK_11870"
     CDS             <1..675
                     /gene="thyX"
                     /locus_tag="ACZPFK_11870"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR1407048.1"
                     /translation="GLTAEKAREFVLRLAEMGHESPIEHVSFTFAIEGVSRSLLAQIT
                     RHRIASYSVQSQRYVRLENFEYVVPPEIEKNEAAKTAYLEMMRAEAESYEKIASLLKE
                     DHKNALMAAGEDEKSATRKAEKMAIEDARFVLPNACDTKMIVTMNARSLYNFFAHRCC
                     SRAQWEIRALAQEMLRLVYPVAPALFAKAGPPCVKGPCPEGRMSCGRASEVRDRYAAL
                     KEELVK"
     gene            679..1341
                     /locus_tag="ACZPFK_11875"
     CDS             679..1341
                     /locus_tag="ACZPFK_11875"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1407049.1"
                     /translation="MGRLIVIEGLDGSGKATQSALLQRALQGQGIDSRSISFPNYASP
                     ASALVRMYLGGEFGSRPEDVNAYAASAFYAVDRFASFKTDWGGFYEGGGVLVADRYTT
                     SNAVHQCSKLPRADWDAYLDWLFDFEYRKIGIPAPDAVLYLDVDPAVSQRLMTGRYAG
                     REEKKDIHERDVAYLEKSRAAAAYCAGRLGWHTIRCCPDGVLRAPEEIASEVLAAARR
                     AL"
     gene            1425..>1984
                     /locus_tag="ACZPFK_11880"
     CDS             1425..>1984
                     /locus_tag="ACZPFK_11880"
                     /inference="COORDINATES: protein motif:HMM:NF021445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR1407050.1"
                     /translation="MQFHEITAADGEWARPRLEGAGFKSCEFAFANVYMWSHVYGTEI
                     AEYQGYVVARNIGRTHHHFLYPVGGTDLEAVLSACVQDALTAGKEPMFYSIPESEVPV
                     IEALFPGKFSFSESRDEQDYLYLSSDLQELPGKIFQKKRNHISRFGKDHPDYVFEPIT
                     PENLPEVRDFNNRWCETYGCDADEGLA"
BASE COUNT          392 a          588 c          674 g          330 t
ORIGIN      
        1 gggctgacgg ccgaaaaggc gcgcgaattt gtgctgcgtc tggcggagat ggggcacgag
       61 agccccatcg agcacgtcag cttcaccttt gccatcgagg gggtgtcccg cagcctgctg
      121 gctcagatca cccgccaccg catcgcctct tacagcgtgc agagccagcg gtatgtgcgg
      181 ctggaaaatt ttgaatacgt ggtgccgcct gagatcgaaa aaaatgaggc ggcgaaaacg
      241 gcgtatcttg agatgatgcg cgccgaggcc gagagctatg aaaagatcgc ctcgctgctc
      301 aaggaggatc acaaaaacgc cctgatggcc gcgggggagg acgagaagtc tgcgacccgc
      361 aaggcggaaa agatggccat tgaggacgcg cgctttgtgc tgcccaacgc ctgtgacacc
      421 aaaatgatcg tcaccatgaa cgcccgcagc ctgtacaact ttttcgcgca ccggtgctgc
      481 agccgggccc agtgggagat ccgggcgctg gcacaggaga tgctgcggct ggtctatccc
      541 gtcgcgccgg ccctgttcgc caaggccggg ccgccctgcg tcaagggccc ctgccccgag
      601 ggcaggatga gctgcggccg cgcctccgag gtgcgggacc gctatgcggc gctgaaagag
      661 gagctggtga agtaagccat gggcagactg atcgtgatcg aagggctgga cggctccggc
      721 aaggcaaccc agagcgccct gctgcagcgg gcgctgcagg ggcagggcat cgacagccgc
      781 agtatcagct tccccaacta tgcatcgccg gccagcgcgc tggtcagaat gtatctggga
      841 ggggagttcg gtagccgccc ggaggatgtg aacgcctatg cggcctccgc cttttacgcg
      901 gtggaccgct ttgcgagctt taagaccgac tggggaggct tttacgaggg gggcggggtg
      961 ctggtcgccg accgctatac cacctccaac gccgtgcatc agtgctccaa gctgccccgg
     1021 gccgactggg acgcctatct tgactggctg ttcgattttg agtaccgcaa gatcggcatc
     1081 cctgcgccgg acgcggtctt gtatctggat gtggacccag ccgtcagcca gcggctgatg
     1141 accgggcggt acgcgggccg cgaggagaaa aaggacatcc acgagcggga cgtggcttat
     1201 ctggaaaaga gccgggccgc cgccgcttac tgcgccggca ggctgggctg gcacaccatc
     1261 cgctgctgcc cggacggcgt tctgcgcgcg ccggaggaga tcgcctcgga ggtgctcgcg
     1321 gcagcccggc gggcactgta gcaaaagggc cggcccaggg gtttcaaaga gaaaacgcgg
     1381 cccgcgcggc cgcaaaaggc aacagatgag aagggaacgg gtttatgcaa tttcatgaga
     1441 tcaccgcggc ggacggcgaa tgggccaggc cgcgcctgga gggcgcgggc tttaagtcct
     1501 gcgagttcgc gttcgccaac gtctatatgt ggagccatgt ctacggcacc gagatcgccg
     1561 aataccaggg ctatgtggtg gcgcgcaaca tcgggcgcac ccatcaccat tttctctacc
     1621 cggtgggcgg cacggacctg gaggcggtgc tgagcgcctg cgtgcaggac gcgctgacgg
     1681 cgggtaagga gccgatgttc tactccatcc cggaaagcga ggtgccggtg atcgaggcgc
     1741 ttttccccgg gaaattctct ttcagcgaga gccgggacga acaggattac ctctatctct
     1801 ccagtgatct gcaggagctg ccgggcaaaa tctttcaaaa aaagcgcaac cacatcagcc
     1861 ggtttggcaa ggaccacccg gattatgtgt ttgagcccat caccccggaa aacctgcccg
     1921 aggtgcgcga tttcaacaac cgctggtgcg agacctatgg ctgcgacgcg gacgaggggc
     1981 tggc
//
LOCUS       DBMDYA010000560         1983 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11696_length_1983_cov_1.347503, whole genome shotgun sequence.
ACCESSION   DBMDYA010000560 DBMDYA010000000
VERSION     DBMDYA010000560.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11696_length_1983_cov_1.347503"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="ACZPFK_11885"
     CDS             <1..331
                     /locus_tag="ACZPFK_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407051.1"
                     /translation="VHGEPAAADSFAAYLTEARDLPAFVPARGESVDLLAARPHLAPA
                     PPLAETAPVTVTAARTPKKKAKPGPLVSAIALLQEMEASGKKLPKRELNELMRAVDRL
                     WGRMQEK"
     gene            536..1099
                     /locus_tag="ACZPFK_11890"
     CDS             536..1099
                     /locus_tag="ACZPFK_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407052.1"
                     /translation="MKTEIRFPACEVENPSGLPYIDELNRMAPFTVALDLPSGWTLRE
                     SNGGETVPPGEFYTPVYLYEGEKLIGYIGFNTFEPYEEEIAPEEYYKTVYPPLRLPSI
                     FHWDPYTPVATGEDGETGVVDIWYLDPDKIDEHPGAMPEVPSLETRGILCYDKGLGVY
                     VGIAFLPGSIEEDQLQAIARSVSLSAA"
     gene            1576..>1983
                     /locus_tag="ACZPFK_11895"
     CDS             1576..>1983
                     /locus_tag="ACZPFK_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1407053.1"
                     /translation="MAKVQVFTNSSSEMSPALAAEFGVIVIPDVVVFSPEEQYLNNVE
                     IDPPTFFQKLQGRKKLPTTSHPNIAQYMDAFSAAGDCDEILFVGLTSKMSGSFSTACT
                     AIRMLQEQGFATPITAYDSLQNCYALCMMALEAA"
BASE COUNT          375 a          594 c          655 g          359 t
ORIGIN      
        1 ggtgcacggc gagcccgcgg cggcggattc gttcgccgct tatctcaccg aggcgcggga
       61 cctgcccgcc tttgtgcccg cccgcgggga gagcgtcgat ctgctggcgg cccggccgca
      121 cctggcgccg gcgccgccgc tggccgaaac cgcgccggtc acagtgaccg cggcccgcac
      181 gccgaaaaag aaagcgaagc ccgggccgct ggtgtccgcc attgcgctgc tgcaggagat
      241 ggaggccagc gggaaaaagc tgcccaagcg ggagctgaac gagctgatgc gcgcggtgga
      301 ccggctgtgg ggccgcatgc aggagaagtg agcaaagacg gttttacagg gcggctaggg
      361 ccggggcggc ccggccgcgg agaggggcaa acagatgaaa aagggacttt ttgtgttggc
      421 ggcgtttttt tgcctggccg cgttggcggc ctgcggcggc ccggccgcct cgggacaggg
      481 gggaatgaaa atggcttcct cggcctctgc tcccgccgtt tcagcggagg cggaagtgaa
      541 gacggagatc cgctttcccg cctgtgaggt ggagaatcca tccgggctgc cctatatcga
      601 cgagctcaac cgcatggcgc cgtttaccgt ggcgctggac ctgccgtccg gctggacgct
      661 gcgggaatcc aatggcgggg agaccgtgcc gccgggggag ttctacacgc cggtctacct
      721 ctacgagggc gaaaagctga tcgggtacat cggctttaac acctttgagc cgtatgagga
      781 ggagattgcg cccgaggagt attacaagac cgtgtacccg ccgctgcggc tgcccagcat
      841 cttccactgg gatccctata cgccggtggc gacaggcgag gacggcgaga cgggcgtggt
      901 ggatatctgg tatctcgacc ccgacaagat cgacgagcac ccgggcgcga tgccggaggt
      961 gccgtcgctg gagacccggg gtatcctctg ctacgacaag gggctcggcg tctatgtggg
     1021 catcgccttt ttgcccggca gcatagagga ggaccaactg caggccatcg cccgcagcgt
     1081 gagcctgtcg gcggcgtgag gctctggcgg tggtcacgag gggcggacgg gccctgcgcc
     1141 cggccggacg gcgaaaagcg ctcaaagggg cggctgcgtc cacctccttt tctcaaaaat
     1201 tttgcgggct cctgccgggc gacctcccgg taggggcccg cttgcgtctg tgaaagtgct
     1261 caacgactgt ttcgagcttc gtcacccggc tccggtaaag ggcccgcccg cgtctgtgag
     1321 ggaggtcgga gaatgccgtc cgccgtcctc ggggcccgtt ctcccacttc tccgcgcctt
     1381 ttcatacccg atccctccgc ggtcaccaaa agcaaaaaac taacaaattt gttggataaa
     1441 gagagactgg agcgcagaat agtgaaaaaa tttgcggaaa gaggtcgttt catatagctt
     1501 tttaaataga aatcagttaa aatgaaaccg attgaagccg caaaatcacc agagaacggt
     1561 caggagggct gctttatggc aaaggtccag gttttcacca attcctcttc cgagatgtcc
     1621 ccggcgctgg ccgcagaatt tggggtcatc gtgatcccgg acgtggtcgt cttcagccct
     1681 gaggagcagt acctgaacaa cgtggagatc gacccgccca ctttttttca aaagctgcag
     1741 gggcgcaaaa agctgcccac cacctcgcat cccaacatcg cccagtacat ggacgctttt
     1801 tccgcggcgg gggactgcga cgagatcctt tttgtcggct tgaccagcaa gatgtccggc
     1861 tcgttcagca ccgcctgcac ggcgatccgc atgctgcagg agcagggctt tgccaccccc
     1921 atcacggctt atgactcgct gcagaactgc tatgcgctgt gcatgatggc gctggaggcg
     1981 gcc
//
LOCUS       DBMDYA010000561         1981 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11709_length_1981_cov_1.214894, whole genome shotgun sequence.
ACCESSION   DBMDYA010000561 DBMDYA010000000
VERSION     DBMDYA010000561.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_1981_cov_1.214894"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            266..1144
                     /locus_tag="ACZPFK_11900"
     CDS             266..1144
                     /locus_tag="ACZPFK_11900"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1407054.1"
                     /translation="MSIWDGLFQGIIQGATEFLPISSDGHLTLYQHLTGNSGEGALFF
                     NLMLHLGTLVAVFVAYRKDIWALIKTFFAMVGDIFRGKFSFKTKDENRRMIFMFVMAC
                     VPLLAFLLVKDWVTAITEDDDIVVEGFCFLWTAVLLFLASKCVKGRKTAREMKVRDAL
                     VVGVFQGTAIMPGISRSGSTISSGLLLGFSKEYCVKFSFILGIPAILGASVLEAKDAF
                     AAGLEIEWAPVLVGIVTAAVVGYLCIRLIQYLVASDKFIIFSYYTAALGVVTLAAGIL
                     EHAWGSNIVELVSRLF"
     gene            1324..1926
                     /locus_tag="ACZPFK_11905"
     CDS             1324..1926
                     /locus_tag="ACZPFK_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407055.1"
                     /translation="MSKSWSILLFGVGAVLLALTFIKGAPAWNTLSGWLFGLFGVITY
                     AVAPVVLYLAFLIAAGRPAKAKLWQGALLLCFASALALIFSRLDLSGGKLWETFLSLG
                     AAGQELRGGGLLSALIGWPLLSLCGRPAANVIIVVVVVVFFMLLTSTTPVDIYRFFRE
                     KASGARGRFDEYERNAAAARAAREERRRQELAEAAAELEA"
BASE COUNT          344 a          569 c          656 g          412 t
ORIGIN      
        1 ggcacggcgg gcaaaagcaa aggaaaataa tgggcgtttt taaaaatttg acgaccaggg
       61 cgcaaaaaag cgcggccctc cggctgtttg ccggcggcgc cgcgcttttt ttattcaccg
      121 cgcccgcgtg cgcctcgacg ctttttgttt tacaagggcc ggttttggtg gtaaaataag
      181 atgtctgggc ccgctctcgg caaagaggaa gccgcgggcc gttttcgccc tgtgcgagag
      241 aaaaatatta cccggaggcg cgtacatgtc gatttgggac ggactttttc aagggatcat
      301 ccagggagcg accgaatttt tgcccatttc cagcgacgga catctcaccc tttaccagca
      361 tctcaccggc aactcgggcg agggcgcgct ctttttcaat ctgatgctcc acctgggcac
      421 gctggtggcg gtatttgtcg cctaccgcaa ggacatctgg gcgctgatca agaccttttt
      481 tgccatggtg ggggatatct tccgcgggaa attcagcttt aagaccaaag acgaaaaccg
      541 gcgcatgatc tttatgtttg tgatggcctg cgtgccgctg ctggcctttt tgctggtgaa
      601 agactgggtc accgccatca ccgaggacga cgatatcgtg gtggagggct tttgcttcct
      661 ctggacggcg gtgctgcttt ttctcgcctc aaaatgcgtc aagggccgca agacggcccg
      721 cgagatgaaa gtgcgggacg cgctggtcgt cggcgtcttt cagggcacgg ccatcatgcc
      781 gggcatctcc cgctccggct ccaccatctc cagcggcctg ctgctgggct tttcaaaaga
      841 gtattgcgtc aagttcagct tcattttggg catcccggcc atcctgggcg ccagcgtgct
      901 ggaggccaag gacgcctttg ctgcgggtct ggagatcgaa tgggccccgg tgctggtcgg
      961 catcgtcacc gcggcggtgg tgggctatct ctgcatccgg ctcatccagt acctggtggc
     1021 cagcgacaaa tttatcatct tttcctacta taccgccgcg ctgggcgtgg ttacgctggc
     1081 ggcgggcatt ctggagcatg cctggggcag caacatcgta gagctggtct cgcggctttt
     1141 ttaagcgcag gggcggggca cagccggccg gtctttgcgc agaaaaagcg ggctcaaaga
     1201 agcggataaa aagaaaaaca gaagtgaaat tacaggggta atcagcgaat ggcagcgaga
     1261 agtcaaaaca gcaggacggc gaccaaaaag agggggaggc cctctaaaaa acagcagccc
     1321 gaggtgagca aatcctggag catcctgctc ttcggcgtcg gggcggtact gctggcgctc
     1381 actttcatca agggggcccc ggcctggaac acgctgtccg gctggctgtt cgggctgttc
     1441 ggcgtcatta catatgcggt ggccccggtg gtgctgtacc tcgcctttct catcgccgcg
     1501 gggcggcccg ccaaggccaa gctctggcag ggggcgctgc tgctctgctt tgcctcggcg
     1561 ctggcgctga tcttttcccg cctcgacctg tccggcggca agctgtggga gacctttctc
     1621 tcgctgggcg cggccgggca ggagctgcgc ggcggcgggc tgctgtccgc gctgatcggc
     1681 tggccgctgc tctccctctg cggccgcccg gcggcgaatg tgatcatcgt cgtggtggtt
     1741 gttgtgtttt ttatgctgct caccagcacc acaccggtgg atatctaccg ctttttccgc
     1801 gaaaaggcgt cgggcgcgcg ggggcggttt gacgagtacg agcgcaacgc cgcggcggcc
     1861 cgcgccgccc gtgaggagcg ccgccggcag gagctggccg aggcggcggc ggagctggag
     1921 gcgtaggagg ccgcgcgcgc ggcggcccgc ggggcggaga tcgacatccc gctgggcggc
     1981 g
//
LOCUS       DBMDYA010000562         1977 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11731_length_1977_cov_1.024520, whole genome shotgun sequence.
ACCESSION   DBMDYA010000562 DBMDYA010000000
VERSION     DBMDYA010000562.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_1977_cov_1.024520"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="ACZPFK_11910"
     CDS             <1..323
                     /locus_tag="ACZPFK_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1407056.1"
                     /translation="AKQPQLPSAFFADNDNIASSCMVSLKKAGYRIPEDVSIIGFDNL
                     PVCKVMEPALSTMSVAKEQLGAYAVDFLIRRIDGSADSPLKIEIKPELVERDSVKQMP
                     AEAE"
     gene            917..1672
                     /gene="larE"
                     /locus_tag="ACZPFK_11915"
     CDS             917..1672
                     /gene="larE"
                     /locus_tag="ACZPFK_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR1407057.1"
                     /translation="MELQSFFAQNPKVALGFSGGVDSSYLLCAGLRCGADVRAYYVKT
                     PFQPQFELDDALRLAAGLGAAVTVLEPELFCRDQVASNPADRCYHCKKALFSALRERA
                     LRDGYPVLIDGTNASDDPGDRPGVRALRELEVRSPLRECGLGKQEIRRLSRREGLFTW
                     DKPAYACLATRVPTGRRITPGLLARIEKAEDALFALGFTDLRVRVYGDAARLQLPPGQ
                     LQRALECRGEITAALRPYFYPVLLDMEGRGTNG"
     gene            1665..>1977
                     /locus_tag="ACZPFK_11920"
     CDS             1665..>1977
                     /locus_tag="ACZPFK_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR1407058.1"
                     /translation="MDKKGTRALLEQVARGTLPVEEALLRLKQQPFEDLGFAKIDHHR
                     ALRQGVAEVIYGAGKTPDQIAQIAAAMLRRGQQTILITRLSPEAAARVGAQLPLEYHE
                     MG"
BASE COUNT          298 a          618 c          734 g          327 t
ORIGIN      
        1 tggctaagca gccgcagctg cccagcgcct tttttgcaga caacgacaac atcgcctcct
       61 cttgcatggt ctcgctgaaa aaggcgggct accgcatccc cgaggacgtc tcgatcatcg
      121 ggttcgacaa cctgccggtc tgcaaggtga tggagccggc cctctccacc atgtcggtgg
      181 ccaaggaaca gctgggggcc tatgcggtgg attttctgat ccggcgcatc gacggctcgg
      241 cggattcgcc gctcaagatc gagatcaagc ccgagctggt ggagcgggac agcgtgaagc
      301 agatgcccgc ggaggcggag tgaggggcag gcgggcggcc ccgccgcctg ggatcgtccc
      361 gagacccgcg ccccgcgggg gcggagcgtg aggcgggccc tcttgctttt tggcgggatt
      421 tatagtataa atgaaacgta cggggcggtg aaagccccgt tgccctcaga agagggcggc
      481 ttgccagatg attttccgcg cgtttttaaa aaaggattgc catgaaggcg gtcgatctcc
      541 ccgggcgcag cgcccgcggg gtattggtcg tgttcatggc atttttttgt gcccggcggc
      601 ccctgtgcgg gctgcgggcc gtgggcggcg gacgggggat cggcgggagc ggaaaacccg
      661 gagaaagcgc ttttcccccg cgggctccgc gctttgcccc gcgcttttcg ccgggcggcg
      721 ctcacagacc ggagaaccgc agcgtgcgga gagcggggac ggcgcgtttc gcggccgtcc
      781 ctctgagcgg gatgaggggc cgcagaggcg gcgtcccgcg ggcgttccgc ctgccggcgg
      841 acggccccaa cgggtcccgg gcggcgggca cccctatttc aattccaatg gcggcgcggc
      901 cgccggggcg gtcttgatgg aactgcaaag cttttttgcc caaaacccca aggtggccct
      961 gggcttttcg gggggcgtgg attcctctta tctgctctgc gcgggcctgc gctgcggcgc
     1021 ggacgtgcgc gcctattatg tcaagacgcc gtttcagccg cagtttgagc tggacgacgc
     1081 gctgcggctg gccgccgggc tgggcgcggc ggtgaccgtg ctggagccgg agttgttctg
     1141 ccgcgaccag gtggcgtcca acccggccga ccgctgctat cactgcaaaa aggcgctctt
     1201 ttccgccctg cgggagcggg cgctgcggga tggatacccg gttttgatcg acggcaccaa
     1261 cgcctcggac gacccgggcg accggcccgg ggtgcgcgcc ctgcgggagc tggaggtgcg
     1321 ctcgcccctg cgcgagtgcg ggcttggcaa gcaggagatc cggcgtctgt cccgccggga
     1381 gggcctgttc acctgggata agccggccta tgcctgcctg gccacccggg tgcccaccgg
     1441 caggcggatc acccccgggc tgcttgcgcg catcgaaaag gccgaggacg cgctgttcgc
     1501 cctgggcttt accgacctgc gggtgcgggt gtacggggac gcggcacggc tgcagctgcc
     1561 gccgggccag ctgcagcgcg cgctggagtg caggggagag atcacggcgg cgctgcggcc
     1621 ctatttttac ccggtgctgc tggacatgga ggggagaggg acaaatggat aaaaagggga
     1681 ccagagcgct gctggaacag gtggcgcggg ggacgctgcc ggtggaggag gcgctgctgc
     1741 ggctcaagca gcagccgttt gaggatttgg gctttgccaa gatcgaccat caccgcgccc
     1801 tgcgccaggg ggtggcggag gtgatctacg gcgcgggcaa aacgccggac cagattgcac
     1861 agatcgccgc ggccatgctg cggcgcggcc agcagaccat cctcatcacc cgcctctccc
     1921 ccgaggcggc cgcccgggtg ggcgcgcagc tgccgctgga gtatcatgag atgggcc
//
LOCUS       DBMDYA010000563         1972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11760_length_1972_cov_1.609834, whole genome shotgun sequence.
ACCESSION   DBMDYA010000563 DBMDYA010000000
VERSION     DBMDYA010000563.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11760_length_1972_cov_1.609834"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="ACZPFK_11925"
     CDS             <1..754
                     /locus_tag="ACZPFK_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR1407059.1"
                     /translation="VNKTVTFTGRSAHAAAAPHEGVDALNAATSAMVNIALQQESFRD
                     EDTIRVHGFVSEGGTAINVIADHVTMQYSVRGKTPEAYRDASLKVDRSLKAAAMATGC
                     GVTIETLPGYMSGVPNYHTEVLDSVLKDLTDEKYNKRFDMGPEMHMTGSDDFGDVATL
                     MPLVHFYSGGYTGSLHNPDVRVTDEYLAYVVTAKIFALTAYRLMKDGAAAAKENIADY
                     KPRMTKAEYLDFMESMLTTETVEPTPLPIVGK"
     gene            1026..>1972
                     /locus_tag="ACZPFK_11930"
     CDS             1026..>1972
                     /locus_tag="ACZPFK_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1407060.1"
                     /translation="MDKIEQKICEIIDSKKEEIMAFGRDVFCHAELGFKEVRTSGKFV
                     EEMKKLGLDAETGLAITGVKSYLKPKGSTEGPTLAVIGEMDALPIPNHPDAWSETGAS
                     HCCGHNAQLTGVVGAAMALADPEVAAAMGGNLCFMAVPAEEFVDVEYKAQLMKDGKLG
                     YGGGKCELIRVGAFDDVDIALGHHIFPGADYGVYNNSSNGFVNKTVTFKGRAAHAAGM
                     PHEGVDALNAATAAMVNIALQQESFRDEDTVRVHGFISGGGTAVNVIADNVTMQYSVR
                     AKTPEAYKDAAAKVDRSLKAAAMATGCGVTIETMAGYLS"
BASE COUNT          400 a          654 c          628 g          290 t
ORIGIN      
        1 tgtcaacaag accgtcacgt tcaccggccg ctcggcccac gccgcggccg cccctcacga
       61 gggcgtggac gcgctgaacg ccgccacctc ggccatggtg aatatcgccc tgcagcagga
      121 gagcttccgc gacgaggaca ccatccgcgt gcacggcttc gtgtccgagg gcggcaccgc
      181 catcaacgtc atcgcggacc atgtcaccat gcagtactcg gtgcgcggca aaacgcccga
      241 ggcctaccgg gacgcctcgc tgaaagtgga ccgctcgctg aaagcggccg ccatggccac
      301 cggctgcggc gtcaccatcg agaccctgcc cggctacatg tccggcgtgc ccaactatca
      361 caccgaggtg ctggacagcg tgctcaagga tctgaccgac gaaaagtaca acaagcgttt
      421 cgacatgggc cccgagatgc acatgaccgg gtcggacgac ttcggcgacg tggccaccct
      481 gatgccgctg gtgcactttt acagcggcgg ctacaccggc agcctgcaca accccgacgt
      541 gcgggtcacc gacgagtacc tggcctatgt ggtcacagcc aagattttcg cgctgaccgc
      601 ctaccggctg atgaaggacg gcgcggccgc ggcaaaggag aacatcgccg actacaagcc
      661 ccgcatgacc aaggcggagt atctggactt tatggagagc atgctcacca ccgagaccgt
      721 ggagcccacc ccgctgccca tcgtcggcaa ataaaagctc cgttgtacag gatcacagtc
      781 ccatccgcgg gcagcgcgcc aaacgcgctg tccgcttttt tgcgcccacg gcgggggagg
      841 gctgttttga gtgcccggcc ctcgcgcctc cccgcgttcc cccgctcttt gccaaagcgg
      901 ggcgggagga gctgttttcg ctggacaatc ccctcgggtg acgctataat ggaaccatcg
      961 gaaaacatcc gcgctggcgc attgaagcgc cagcggggaa aacgcattca gggagggacg
     1021 gaatcatgga caagatcgaa caaaaaatct gtgagatcat cgacagcaaa aaagaggaga
     1081 tcatggcctt tggccgggac gtcttctgcc acgccgagct gggcttcaaa gaggtgcgca
     1141 ccagcggcaa gttcgtcgag gagatgaaaa agctggggct ggacgccgag acgggcctcg
     1201 ccatcaccgg cgtcaagagc tatctgaaac ccaagggctc caccgagggg ccgaccctcg
     1261 ccgtgatcgg cgagatggac gcgctgccca tccccaacca cccggacgcc tggtctgaga
     1321 ccggggcctc ccactgctgc ggccacaacg cccagctcac cggcgttgtg ggcgcggcga
     1381 tggcgctggc cgacccggag gttgcggcgg ccatgggcgg caacctctgc tttatggcgg
     1441 tgccggccga ggagtttgtg gacgtggagt acaaggcgca gctgatgaaa gacggcaagc
     1501 tgggctacgg cggcggcaag tgcgagctga tccgggtggg cgcgttcgac gacgtggaca
     1561 tcgccctggg ccatcacatc ttccccggcg cggactacgg cgtctacaac aacagcagca
     1621 acggctttgt caacaagacc gtcacgttca aggggcgggc cgcccatgca gccggtatgc
     1681 cccacgaggg cgtggacgcg ctgaacgccg ccaccgcagc catggtgaac atcgccctgc
     1741 agcaggagag cttccgcgat gaggacaccg tgcgggtgca cggcttcatc tccggcggcg
     1801 gcaccgcggt caacgtcatc gcggacaacg tcaccatgca gtactcggtg cgcgccaaga
     1861 cgcccgaggc ctacaaggac gcggccgcaa aggtggaccg ctcgctgaaa gcggccgcca
     1921 tggccaccgg ctgcggcgtc accatcgaga caatggccgg ctacctgtcc aa
//
LOCUS       DBMDYA010000564         1970 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11772_length_1970_cov_1.390583, whole genome shotgun sequence.
ACCESSION   DBMDYA010000564 DBMDYA010000000
VERSION     DBMDYA010000564.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_1970_cov_1.390583"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1970
                     /locus_tag="ACZPFK_11935"
     CDS             <1..>1970
                     /locus_tag="ACZPFK_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1407061.1"
                     /translation="QSLLKDLAGLIHRSIKFTASTTAVADRIEKEIAALTAANEGLHV
                     TPLMNQGDYIHLIIDSVLQNLVIGGLLAILVLIFFLKDAKPTLIIACSIPISLLFAIT
                     LMYFTGVTLNIISLSGLALGVGMLVDNSIVVIENIYRLRSEGAPAHKAAVAGANQVAG
                     AIFASTLTTVCVFLPIVFTQGISRELFTDMGLTIGYSLMASLIVALTLVPALGSTVLK
                     TTVEKKHPWFDAVVRGYEKLLRFSLAHRAPVLLAAVALLGLACYGAATMGTAFIPAMD
                     SPQMSATLELPKGTDTRTLYETTDEVVARMLAVDGVETVGAMEGGGMMGGSSSGGMSF
                     YILLGDDVKATNAEVARQLRDAVADLDCTLEVSASTMDMSALGGSGIEIELKGRDLDQ
                     LQAAADEVAALVESTEGTLEVSTGSGEADPEMRVTVDRDKAMRKGLTTAQVYAELAAA
                     LKAETTATTLTQGQNDYPVIVVGGADALTRGSLADYRFTVTNAEGEEESFPLSDIAEI
                     SERPGVSAIRRDAGSRYRTVSAGIDADHNVGLVSRELEKKLAAYQPPAGVTVTLQGES
                     ETINSAMGDLVKMILLAVVFIYLIMVAQFQSLLSPFIVLFTLPLAFTGGLLLLWVTGK
                     ELSISAMLGFLVLAGVVVNNGIVFVDYANQLRL"
BASE COUNT          333 a          671 c          647 g          319 t
ORIGIN      
        1 caaagcctgc tcaaagacct tgcgggcctc atccatcgtt ccataaaatt caccgcctcc
       61 accaccgccg tggccgaccg catcgaaaag gagattgcgg ccctgacggc ggcaaacgag
      121 ggcctgcacg tcaccccgct gatgaaccag ggcgactaca tccacctgat tatcgacagc
      181 gtgctgcaaa acctggtcat cggcggcctg ctggcgattc tggtgctgat cttcttcctc
      241 aaggatgcga agcccaccct catcatcgcc tgcagcatcc ccatcagcct gctgttcgcc
      301 atcacgctga tgtacttcac cggcgtgacg ctgaacatca tctccctgtc cggcctggcg
      361 ctgggcgtgg gcatgctggt ggacaacagc atcgtggtca tcgagaacat ctaccgactg
      421 cgcagcgagg gggcgcccgc ccacaaggcg gccgtggcgg gcgcaaacca ggtggcgggc
      481 gcgatcttcg cctccaccct caccacggtc tgcgtctttt tgcccatcgt cttcacccag
      541 ggcatctccc gcgagctgtt caccgacatg ggcctcacca tcggctacag cctgatggcc
      601 agcctgatcg tcgccctgac gctggtgccg gcgctgggct ccacggtgct caagaccacg
      661 gtggaaaaaa agcatccctg gtttgacgcc gtggtgcgcg gctatgaaaa gctgctgcgc
      721 ttctcgctgg cccaccgggc tccggtgctg ctcgccgcgg tggccctgct gggcctggcc
      781 tgctacgggg cggccaccat gggcaccgct ttcatcccgg ccatggacag cccgcagatg
      841 agcgccacgc tggagctgcc caagggcacg gacacccgca ccctgtacga gaccaccgac
      901 gaggtggtgg cgcggatgct cgcggtggac ggggtcgaga ccgtgggcgc catggagggc
      961 ggcggcatga tgggcggctc ctcctccggc ggcatgagct tttatatcct gctgggcgac
     1021 gacgtaaagg ccaccaacgc cgaggtcgcc cggcagctgc gggacgcggt ggccgacctg
     1081 gactgcacgc tggaggtgag cgcctccacg atggatatgt cggccctggg cggcagcggc
     1141 atcgagatcg agctcaaggg ccgcgacctg gaccagctgc aggcggcggc cgacgaggtg
     1201 gcggccctgg tggagagcac cgagggcacc ctggaggtct cgaccggcag cggcgaggcg
     1261 gacccggaga tgcgggtcac ggtggatcgg gacaaggcga tgcgcaaggg gctgaccacg
     1321 gcccaggtct acgccgagct ggccgcggcg ctcaaggccg agaccaccgc caccaccctc
     1381 acccaggggc agaacgacta tccggtgatc gtggtgggcg gagccgacgc cctcacccgc
     1441 ggcagcctgg ccgattaccg gttcaccgtc accaacgccg agggcgagga ggagagcttc
     1501 ccgctgtccg acatcgccga gatcagcgag cggcccggcg tctccgccat ccggcgcgac
     1561 gcgggctccc gctacaggac ggtcagcgcc ggcatcgacg ccgaccacaa cgtgggcctg
     1621 gtcagccgcg agctggaaaa gaagctggcg gcctaccagc cgcccgcggg cgtcacggtg
     1681 acgctgcagg gcgagagcga gaccatcaac agcgccatgg gggatctggt caagatgatc
     1741 ctgctggcgg tggtgttcat ctacctcatc atggtggccc agttccagag cctgctctcg
     1801 ccgttcatcg tcctgttcac cctgcccctg gctttcaccg gcggcctgct gctgctgtgg
     1861 gtcaccggca aagagctgtc catcagcgcc atgctgggct ttctggtgct ggcgggcgtg
     1921 gtggtcaaca acggcatcgt ctttgtggat tacgccaacc agctgcgcct
//
LOCUS       DBMDYA010000565         1968 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11780_length_1968_cov_1.448313, whole genome shotgun sequence.
ACCESSION   DBMDYA010000565 DBMDYA010000000
VERSION     DBMDYA010000565.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11780_length_1968_cov_1.448313"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(137..1267)
                     /gene="ftsZ"
                     /locus_tag="ACZPFK_11940"
     CDS             complement(137..1267)
                     /gene="ftsZ"
                     /locus_tag="ACZPFK_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1407062.1"
                     /translation="MAFILDKETENVTNIKVIGVGGGGGNAVNRMVDSGLKGVEFISM
                     NTDQQALSASHATHKVQLGAKLTRGRGAGADPEVGKRSAEETRDEIASALKGAQMIFI
                     TAGMGGGTGTGAAPVVASIAKEMGILTVGIVTKPFAFEGKRKMSLAEEGIANLREQVD
                     SLIVIPNERLKLISQEKITLLNAFEAADNVLRQGVESISALINIPAFINLDFADVRSI
                     MKDAGHAHMGVGSATGHTKAEESAKAAISSPLLETSISGARGVIINITSSPDIGLEEV
                     ETAASLITTSAHPEANIIWGAAFDEAMSDEMRITVIATGFENPPSSLAGEKSEPQKGA
                     SDSAFDINLQFSGETPAEGGSGFDDDNSYYDDVLTLLNNHGK"
     gene            complement(1521..>1968)
                     /locus_tag="ACZPFK_11945"
     CDS             complement(1521..>1968)
                     /locus_tag="ACZPFK_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1407063.1"
                     /translation="VFLVTPAVEAYYIESEGSALLLSENLKVLNTAAPAPEGLCRLDG
                     VSASAPVAGKPFAAADETSSELVKTVLGAVRQSGLGQVVSVDFTDPYELSLNYAGRIT
                     VGLGTTAQLDYKLEIVKKTLEDPYFTDTTTGALDASDAGKAVFQPG"
BASE COUNT          335 a          615 c          595 g          423 t
ORIGIN      
        1 gtcacacatt atagcatccc ccgcccccga acaaaaggga tttttctcaa aaaagggggg
       61 acggcggccc tttgccaaaa attagcaagg cgggcgcgcc cccttggccg gagcgcgccc
      121 gcgatctgat ccattcttat ttgccgtggt tgttcagcag ggtcaggacg tcgtcgtaat
      181 agctgttgtc gtcgtcaaag ccggagccgc cctctgccgg tgtctcgccg gaaaactgca
      241 ggttaatgtc aaaagctgaa tcggaagcgc ccttttgcgg ctctgatttt tcgcctgcca
      301 gcgaggaggg cgggttctca aagccggtgg cgatgacggt gatgcgcatc tcgtcgctca
      361 tcgcctcgtc aaaggcggcg ccccagatga tattggcctc ggggtgcgcg gaggtggtga
      421 tgagggaggc ggccgtctcc acctcctcca ggccgatatc cggcgaggag gtgatgttga
      481 tgatcacgcc gcgggcgccg gagatgctgg tctccagcag cggggaggag atggcggcct
      541 tggccgactc ctccgccttg gtgtggccgg tggcggagcc cacgcccatg tgcgcgtggc
      601 ccgcgtcttt catgatgctg cgcacgtccg caaaatccag attgatgaaa gcggggatat
      661 tgatgagggc cgagatgctc tcgacgccct ggcgcagcac gttgtccgcc gcctcaaagg
      721 cgttgagcag ggtgatcttc tcctggctga tcagcttcag gcgctcgttg gggatgacga
      781 tcaggctgtc cacctgctcg cgcaggttgg cgatcccctc ctccgccagg ctcatcttgc
      841 gcttgccctc aaaggcaaag ggcttggtga caatgcccac ggtgaggatg cccatctcct
      901 tggcgatgga ggccaccacc ggcgcggccc cggtgccggt gccgccgccc atgcccgcgg
      961 tgataaagat catctgcgcg cctttcaggg ccgacgcgat ctcgtcccgc gtctcctccg
     1021 cgctgcgctt gcccacctcc gggtcggcgc ccgcgccgcg gccgcgggtg agctttgccc
     1081 ccagctgcac cttgtgggtt gcatgggagg cggaaagggc ctgttggtcg gtgttcatcg
     1141 aaataaactc gacgcccttg aggccggagt ccaccatgcg gttgactgcg ttgccgccgc
     1201 cgccgcccac gccgatcacc ttgatgttgg tgacgttctc cgtctctttg tcgagaatga
     1261 aagccatttg aaattccccc tacagcgtct gttgccgaaa cggcggccgg cgggacggcc
     1321 tgtcccgttc cgccctgtat ccgcgcatct atatttagtg gttttatatc aaagcattct
     1381 tgcagcactc tatatattac tttatttaga gtaacagatt ttctaaaaca attcaatagt
     1441 ttttgcaata atgtacaaat ttcgctcccc gtttttgttt acaaacgggc agttgggaaa
     1501 attttcgaca gcgttttcac tcagccgggc tgaaagaccg ccttgcccgc gtcgctggcg
     1561 tccagcgcgc ccgtggtggt gtcggtgaaa taggggtctt ccagtgtttt tttgacgatc
     1621 tccagcttgt aatccagctg ggcggtggtg cccagaccca ccgtgatgcg ccccgcgtag
     1681 ttgagcgaga gctcatacgg gtcggtgaag tccaccgaga ccacctgccc cagcccgctc
     1741 tgccgcaccg cccccagcac cgttttcacc agctcgctgg aggtctcgtc cgccgcggca
     1801 aacggcttgc ccgccaccgg cgcggacgcg gaaacgccgt ccagacggca gagcccctcc
     1861 ggcgccggcg cggcggtgtt cagcactttc aggttttcgc tgagcagcag cgcgctgccc
     1921 tcgctttcga tgtaataggc ctccactgcc ggcgtgacca gaaacacc
//
LOCUS       DBMDYA010000566         1967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11788_length_1967_cov_1.228832, whole genome shotgun sequence.
ACCESSION   DBMDYA010000566 DBMDYA010000000
VERSION     DBMDYA010000566.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11788_length_1967_cov_1.228832"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..176)
                     /locus_tag="ACZPFK_11950"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="ACZPFK_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491642.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            339..1592
                     /locus_tag="ACZPFK_11955"
     CDS             339..1592
                     /locus_tag="ACZPFK_11955"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1407064.1"
                     /translation="MIQDFFDVSEELLEIDRQAMAQCAARFAAIEEVKEYNQLKMLKA
                     FTSCGVAGSHLVGTTGYGYDDRGRDTLDKVFAAAVGAQDALCRPHFLSGTHALAVALF
                     GVLRAGDTLFAATGRPYDTLGGVIGLDGSKCGSLAEFGVRYDEAELGQDGSPDLVAVA
                     EKSRGAKVCYIQRSRGYASRPALSLQTIGAIAQAARRANPDVIVMVDNCYGEFTQKAE
                     PVSVGADLMIGSLIKNAGGGIAPTGGYIAGRADLVELCGHRLTAPGTGRELGCTMDVL
                     RQLYLGLYYAPGVTAEALKTSVYASSLFSLLGYKTSPAYDADRNDIITSIETGSPEGL
                     VAVCQGVQAGSPVDSFVTPEPCPMPGYDAEVIMAAGAFTMGSSIELSCDGPMRAPYTA
                     YLQGGLNFAASRAGLLLAATRLKKG"
BASE COUNT          311 a          660 c          705 g          291 t
ORIGIN      
        1 gtctggaggg gtacatcgac cggctcgcgg ctgaccacgc cgctggcctc gtgctcaatg
       61 gggcgggtct cgttggtctc gatggggccg aggccgtcaa tgggctggcc cagcacgtcc
      121 accacccggc cgatcaggcc gtcgcccacg gggacgtcgg cggcccgccc ggtgcggctg
      181 ctctgcgaag cgccgctgcg cgggttttat gaaaggctgg gttttgccgc ctgcggcacg
      241 gcgctggagg cggtttgccc ccctggggag ggggacggaa agacgataaa aagccccttt
      301 tgagtgggga cggaagagca aaggagaacg cggaacagat gatccaggat ttttttgacg
      361 tttcggagga gctgctggag atcgaccggc aggctatggc ccagtgcgcg gcgcggtttg
      421 ccgccatcga ggaggtcaag gagtacaatc agctgaagat gctgaaggct ttcaccagct
      481 gcggcgtggc gggcagccac cttgtcggca ccaccggcta cggctacgac gaccgcggcc
      541 gggacacgct ggacaaggtg ttcgccgccg cggtgggggc gcaggacgcc ctctgccgcc
      601 cgcatttcct ctccggcacc cacgccttgg cggtggcgct gttcggggtg ctgcgggccg
      661 gggacaccct gtttgccgcc accgggcggc cttacgacac gctgggcggg gtcatcgggc
      721 tcgacggcag caaatgcggc agcctggctg agttcggcgt gcgctacgat gaggcggagc
      781 tcggccagga cggcagcccc gatcttgtgg cggtggcgga gaagagccgg ggcgcaaagg
      841 tctgctatat ccagcgcagc cggggctatg ccagccgccc ggccctgtcg ctgcagacca
      901 tcggcgccat cgcccaggcg gccaggcggg cgaacccgga tgtgatcgtg atggtggaca
      961 actgctacgg cgagttcacc cagaaagcgg agccggtgag cgtgggcgcc gacctgatga
     1021 tcggcagctt gatcaaaaat gcgggcggcg gcatcgcccc caccggcggc tatatcgccg
     1081 gccgggccga tctggtggag ctgtgcggcc accgcttgac cgcccccggc accgggcggg
     1141 agctgggctg caccatggac gtgctgcgcc agctgtactt gggcctttac tacgcccccg
     1201 gcgtcaccgc cgaggcgctc aagaccagcg tctacgccag cagcctgttc agcctgctcg
     1261 gctacaagac ctcgccggcc tacgacgcgg accgcaacga catcatcacc agcatcgaga
     1321 ccggcagccc cgaggggctg gtggccgtct gccagggggt gcaggcgggc agcccggtgg
     1381 acagcttcgt caccccggag ccctgcccca tgcccggcta cgacgccgag gtcatcatgg
     1441 cggccggggc gttcacgatg ggcagcagca tcgagctgtc ctgcgacggc cccatgcgcg
     1501 cgccctatac ggcctacctg cagggcgggc tcaactttgc ggccagccgc gccgggctgc
     1561 tgctggccgc cacacggctg aaaaagggct gagctctgcg gcaaggcgga tcgctttaca
     1621 gacaaaaaag gggggagggc cggcggccct cccccctttt ttgtccgcgc cgggctcctg
     1681 ccggggagcg cgggccggtg gcggccacgg gcccgcgctt ggcgaaaaag ggaacgcacc
     1741 cggcggaaaa gggctgcgcc cggcggcggc tttccggcct gtgcggcagg cccagggcag
     1801 aaagccgccg cttggcacgc ccaacggctc gcagcgcccc gtttgcttgc aaaccggttt
     1861 ttcccctgaa cagctgatgt ctcccctact tgaccgatcg gccaaaaccg cagctttccc
     1921 ggcaggccag aaccgcacgc gcccggtcgg ccgaagccgc gcgcgcc
//
LOCUS       DBMDYA010000567         1966 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11797_length_1966_cov_1.284182, whole genome shotgun sequence.
ACCESSION   DBMDYA010000567 DBMDYA010000000
VERSION     DBMDYA010000567.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11797_length_1966_cov_1.284182"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            101..>1966
                     /gene="ileS"
                     /locus_tag="ACZPFK_11960"
     CDS             101..>1966
                     /gene="ileS"
                     /locus_tag="ACZPFK_11960"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1407065.1"
                     /translation="MRAGLPAKEPVMLAQWLADDLYHEIVKSNEGKPLYVLHDGPPYA
                     NGKIHMGTAMNKVLKDIIVRYKNMAGFCSPYVPGYDTHGLPIELKALEAAGDNKANVT
                     QLELREACRDFALKHVDIMSDEFQRLGVVGDFKNPYLTLAPEFEATQVRIFGEMAKKG
                     YIYKGKKAVYWCPTDKTALAEAEIEYSDDECDSIYVAFQVVKDNGALAQAGIPMEKTR
                     FVIWTTTTWTLPANLAICLGPDFDYVFVKVGDTYYVMAEALVESTMNACGISEYEVQD
                     VVLKGSAFELMETKHPFLDRNSLLIVGDHVTLDSGTGCVHTAPGHGVEDFEVCQNHYP
                     QIPVICPVDEAGYLTEEAGPFAGLNVLGDANKVILAHIKESGHLLGVQHMTHQYPHCW
                     RCHKPIIFRATEQWFCSVEAFKDATYQAIDSVNWLPAWGKERITGMVRDRNDWCISRQ
                     RTWGVPIPVFYCKKCGKYHITESSINTVAELFAKEGSDAWYKYSADEILHGTETCACG
                     GTEFEKDRDIMDVWFDSGSSHMAVLETRPELCWPCDLYIEGGDQYRGWFQSSLLTSVA
                     VRGVAPYKNVISCGWVVDGQGRAMHKSLGNGIAPEEIIKDYGADIIRLWAASSDYQ"
BASE COUNT          409 a          616 c          602 g          339 t
ORIGIN      
        1 tccgggcgac cggtagacca agagacagag attgaggaga ggattatcag tgcaggaaga
       61 ttacaacaag accctgaatc tgcccaaaac ggatttcagc atgcgcgcgg ggctgcccgc
      121 caaggagccg gtgatgctgg cccagtggct ggcggacgac ctctatcacg agatcgtcaa
      181 gagcaatgag ggcaagcccc tctatgtgct tcacgacggc ccgccgtatg ccaacggcaa
      241 gatccacatg ggcaccgcga tgaacaaggt cctcaaggac atcatcgtgc gctacaaaaa
      301 tatggcgggc ttctgctcgc cctatgtgcc gggctacgac acccacggcc tgcccatcga
      361 gctgaaagcg ctggaggcgg cgggcgacaa caaggccaac gtgacccagc tggagctgcg
      421 cgaggcctgc cgcgattttg cgctcaagca tgtggacatc atgtcggacg agtttcagcg
      481 gctgggcgtc gtgggcgatt tcaaaaaccc gtatctgacc ctcgcgccgg agtttgaggc
      541 cacccaggtg cgcatcttcg gcgagatggc gaaaaagggc tatatctata agggcaaaaa
      601 ggccgtctac tggtgcccca ccgacaagac cgccctggcc gaggcggaga tcgagtacag
      661 cgacgacgag tgcgactcca tctatgtggc tttccaggtc gtcaaggaca acggcgcgct
      721 ggcccaggcg ggtatcccga tggaaaagac ccgcttcgtg atctggacca ccaccacctg
      781 gacactgccc gccaacctgg ccatctgctt gggcccggat ttcgactatg tctttgtcaa
      841 ggtaggggat acttactacg tcatggccga ggcgctggtg gaatccacca tgaatgcttg
      901 cggcatcagt gagtacgagg tgcaggacgt ggtgctcaag ggcagcgcgt tcgagctcat
      961 ggagaccaag cacccgttcc tcgaccgcaa ctccctgctc atcgtcggcg accacgtcac
     1021 gctggattcc ggcacgggct gcgtccacac cgctccgggc cacggcgtcg aggactttga
     1081 ggtctgccag aaccactatc cccagatccc cgtcatctgc cccgtggacg aggcgggcta
     1141 cctgaccgaa gaggcggggc cctttgcggg cctgaacgtg ctgggggatg ccaacaaggt
     1201 cattctggcc cacatcaaag agagcggcca cctgctgggc gtgcagcaca tgacccacca
     1261 gtatccccat tgctggcgct gtcacaagcc catcatcttc cgcgccaccg agcagtggtt
     1321 ctgcagcgtg gaggcgttca aggatgcgac ctaccaggcc atcgacagcg tcaactggct
     1381 gcccgcctgg ggcaaagagc gcatcaccgg catggtgcgc gaccgcaacg actggtgcat
     1441 ctcgcgccag cgcacctggg gcgtgcccat cccggtgttc tactgcaaaa agtgcggcaa
     1501 ataccacatc accgagtcct ccatcaacac ggtcgccgag ctgttcgcca aagagggcag
     1561 cgacgcttgg tacaagtact ccgccgacga gatcctgcac ggcaccgaga cctgtgcctg
     1621 cggcggcacc gagtttgaga aagaccggga tatcatggac gtgtggtttg attccggctc
     1681 ctcccacatg gcggtgctgg agacccgccc cgagctgtgc tggccctgcg acctgtacat
     1741 cgagggcggc gaccaatatc gcggctggtt ccagtcctcg ctgctgacca gcgtggcggt
     1801 gcgcggcgtc gcaccctaca aaaacgtcat cagctgcggc tgggtggtgg acggccaggg
     1861 ccgcgccatg cacaagtcgc tgggcaacgg cattgccccc gaggagatca tcaaggacta
     1921 cggcgctgat atcatccgcc tgtgggccgc ctcgtctgat tatcaa
//
LOCUS       DBMDYA010000568         1960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11834_length_1960_cov_1.381926, whole genome shotgun sequence.
ACCESSION   DBMDYA010000568 DBMDYA010000000
VERSION     DBMDYA010000568.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11834_length_1960_cov_1.381926"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="ACZPFK_11965"
     CDS             complement(<1..670)
                     /locus_tag="ACZPFK_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1407066.1"
                     /translation="MHSRKKQQNKPREDKQRSAVDAPFLIIVLILVVFGLIMLFSASY
                     ATALYRFGDSFHFIKDQVLFAVVGVTAMFIASRVDYHIFHRLAWPLMVVSLLLLVVVL
                     FMPALNNAHRWIQIPGLGTLQPSEIAKFAVIVLFAHIISINYAKMKTFSYGVIPFMVV
                     LVPIIVLMILEPHLSGTILIVAIGGIMMFVGGTGLKWFGLGGGLVAGGITAAVLAFPD
                     LVPYA"
     gene            complement(723..1736)
                     /gene="mraY"
                     /locus_tag="ACZPFK_11970"
     CDS             complement(723..1736)
                     /gene="mraY"
                     /locus_tag="ACZPFK_11970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1407067.1"
                     /translation="MARFVLIIAALLSLAVTAASGFFILPWLKKLHYGQTIKEIGPTW
                     HNKKQGTPMMGGLMFILGSTVAIATAFTTLAVTEPDMMGGMQAQQNRTLLIAVLATFG
                     FSFVGFVDDYLKVVHKQNLGLMARYKIVMQVVVATAFLVGMQLNGTLSTALSLPIFGR
                     LELGYWFYPLAYILIIGFVNAVNLTDGLDGLSSSVTFVAMLGYMTIATLLGYHTMGIF
                     GAAMAGGCAGFLFWNFYPAKVFMGDTGSMFLGGAVVTISFGVGHPELLFLLGIIYLAE
                     AASVMIQVTYFKLTHGKRIFKMTPIHHHFEMCGWSEVKIDGVFAFVTLIGVVLGFVYI
                     YLN"
BASE COUNT          419 a          612 c          597 g          332 t
ORIGIN      
        1 tggcgtaggg caccagatcg ggaaaggcca gcaccgccgc ggtgatgccc cccgccacca
       61 gaccgccgcc caggccgaac catttcagcc ccgtgccgcc cacgaacatc atgatgccgc
      121 cgatggccac gatcagaatc gtgccggaga ggtgcggctc caggatcatc agcacgatga
      181 tcggcaccag caccaccata aacgggatga cgccgtagga gaaagtcttc atctttgcgt
      241 agttgataga gatgatgtgg gcaaagagga cgatgacggc aaacttggcg atctcgctgg
      301 gctgcagggt gcccaggccc gggatctgga tccagcggtg ggcgttgttc agcgccggca
      361 tgaagagcac caccaccagc agcagcagcg agacgaccat cagcggccag gccagccggt
      421 gaaagatatg gtagtccacc cgcgaggcga tgaacatggc cgtcaccccg accaccgcga
      481 agagcacctg gtccttgata aagtgaaagc tgtcgccaaa gcgatagagc gcggtggcgt
      541 aggaggcgga aaaaagcatg atcaggccga agaccaccag gatcagcacg atgatgagaa
      601 acggcgcgtc caccgccgag cgctgcttgt cttcgcgggg tttgttctgt tgttttttgc
      661 ggctgtgcaa tgcgcgtttc tccttttgcc gtgtttttcc ccgccgccct gcggccgggg
      721 gatcagttca gatagatgta gacaaagccc agcaccacgc cgatcagggt gacaaaggca
      781 aagaccccgt cgatcttgac ctcgctccag ccgcacatct caaaatggtg atggatgggg
      841 gtcatcttga aaatgcgctt gccgtgggtc agcttgaagt aggtgacctg gatcatcacc
      901 gaggccgcct cggccagata gatgatgccc agcaggaaca gcagctcggg gtggccgacg
      961 ccgaacgaga tggtcaccac cgccccgccc aggaacatgc tgcccgtatc ccccatgaac
     1021 accttggcgg ggtaaaagtt ccagaacaaa aagcccgcgc agccgccggc catggccgcg
     1081 ccgaagatgc ccatcgtgtg atagcccagc agggtggcga tggtcatgta ccccagcatc
     1141 gccacaaagg tgacgctgga ggacaggccg tcgagcccgt cggtgagatt gaccgcgttg
     1201 acaaagccga tgatcaaaat gtacgcgagc gggtagaacc aatagcccag ctccagccgc
     1261 ccgaagatgg gcaggctcag cgcggtggac agggtgccgt tgagctgcat gcccaccaga
     1321 aaagcggtgg ccaccaccac ctgcatcacg atcttgtagc gcgccatcag gcccaggttc
     1381 tgcttgtgca ccaccttgag gtagtcatcc acaaagccca cgaaagaaaa gccgaaagtc
     1441 gccagcaccg cgatgagcag ggtgcggttc tgctgcgcct gcatcccgcc catcatatcc
     1501 ggctcggtga ccgccagggt ggtgaacgcc gtggcgatcg ccaccgtgct gcccaggatg
     1561 aacatcaggc cgcccatcat cggcgtgccc tgttttttgt tgtgccaggt ggggccgatc
     1621 tccttgatag tctgcccgta gtgcaatttt ttaagccagg gcaggataaa aaagccggag
     1681 gccgccgtga cggcgagcga cagcagcgcc gcgatgatca gcacaaatct tgccatttta
     1741 ccgattcctt cctctccccc ggcccgcgcc ggggttcgct tccgttttct ttttcagcgc
     1801 cgcgccagac gggtcagagc gggcggccgc aatacagctc tgcgatctcc tcaaaggcca
     1861 tgccgcggct ggccttgaac agcaccgtgt ccccgggccg gcaataggcc cgcagcagcg
     1921 ccgcggcgtc ctcctttgcc gcgcaggggt gtacctccgc
//
LOCUS       DBMDYA010000569         1959 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11838_length_1959_cov_1.350915, whole genome shotgun sequence.
ACCESSION   DBMDYA010000569 DBMDYA010000000
VERSION     DBMDYA010000569.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11838_length_1959_cov_1.350915"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="ACZPFK_11975"
     CDS             <1..398
                     /locus_tag="ACZPFK_11975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR1407068.1"
                     /translation="HFLRCDYAQKSLTVFFNVKDWALNPEGTMHGGLISTVFDGTFGA
                     LCHYWGKQRFITTVSLSTTFLKPVLPGDTLVVTAKATSIGRTLMSMTAEGTTHDGERL
                     AATAFTTFMFLDKEFSREGWMCPPERDAL"
     gene            496..1347
                     /locus_tag="ACZPFK_11980"
     CDS             496..1347
                     /locus_tag="ACZPFK_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HIR1407069.1"
                     /translation="MDALRVLVVEDESREAALLEDYLASLQDFQPAGIADGQARALSM
                     VVERSPDIVLLDLRLRQGDGTGFLSDLEALHLAYKPFILVVSRLLTLATQTTVCGLGA
                     DYCVSKDNIDCSPRGVVGLLRNFEPFIRSHTAVDLQVNEHPITPQLQEKQLRRLMALT
                     LARISIPTGDLVNRELVEAMFFVLPILEKDLPLPDLKNTVYPLIAEKYEIKDPRSIER
                     GIRNILEKVWDRTEKTLLKNYYDFPVSKDTGYPSPREFIFNFAYANFTSLPREKAPIV
                     AIENVAK"
     gene            1687..1902
                     /locus_tag="ACZPFK_11985"
     CDS             1687..1902
                     /locus_tag="ACZPFK_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407070.1"
                     /translation="MFELLQETFTDEDGISTRCFSLVDRGRQLKIPYLCTDRCKAEIL
                     QERLNSEALPPFQLQNLIEDYIQSLYM"
BASE COUNT          467 a          506 c          465 g          521 t
ORIGIN      
        1 cgcattttct gcgctgcgac tatgcgcaaa aaagcctcac cgtgtttttc aacgtcaaag
       61 actgggctct caaccccgag ggcaccatgc acggcggcct gatctccacc gttttcgacg
      121 gcactttcgg ggcgctgtgc cactattggg gcaaacagcg gttcatcacg acggtcagcc
      181 tttccaccac ctttttaaag ccggttttgc ccggcgacac cctggtggtc accgcaaagg
      241 cgacttctat cggccgcacc ctgatgagca tgacggcgga gggcaccacc catgacggcg
      301 agcgcctggc ggcaaccgcc tttaccacct ttatgttttt ggataaggag tttagccggg
      361 aggggtggat gtgtcccccg gaaagggatg cattataaaa aagtttaatt ttgtcccatt
      421 ttttatccac aaatcgtcaa aatatgataa aataatacaa acaagctttt atcaacaata
      481 aaaaggatga gaaatatgga tgccctccgt gttttagtcg tggaagacga atcccgcgag
      541 gcagccttgc tggaagacta tcttgccagc ctgcaggact ttcaaccggc cggcatcgca
      601 gatgggcagg cgcgtgcgct ttccatggtc gtggagcgat cccccgacat cgtcctgctg
      661 gatcttcggc tgcgccaagg ggatgggacc ggctttctct ctgatctgga agcgctgcat
      721 ctggcctata agccttttat cctggtggtt tctcgtctgc tcacccttgc cacccagacg
      781 acggtctgcg gccttggcgc cgattattgc gtttcaaagg acaatattga ctgctccccg
      841 cggggtgtcg tgggcttgtt gcggaacttt gagcctttta tccgcagcca cacggcggtc
      901 gatctgcagg tcaacgagca ccccatcacc cctcaactgc aagaaaagca gctgagacgg
      961 ttgatggccc tcacgctggc ccgcatctct atccccaccg gcgatctcgt caaccgcgag
     1021 ctggtggaag cgatgttttt tgtgttgccc atcctggaaa aggatcttcc cctgcccgat
     1081 ttaaaaaaca cggtctatcc cttgattgca gaaaaatatg aaatcaagga tccgcgctca
     1141 attgagcgcg gaatccgcaa tatcctggaa aaagtatggg accgcaccga aaaaacactt
     1201 ttaaaaaatt actacgattt tcccgtcagc aaggataccg gttatccttc tccccgcgag
     1261 ttcatcttca attttgctta cgctaatttc acctctctgc cgcgtgaaaa agcgccgatc
     1321 gtcgccattg aaaatgtggc aaaataaaat tgtgttttct cttgagaaag ggcctttatc
     1381 caaaaattga aaagccgtgc gtcttgtagc ttttgggcaa gagacgtgcg gcttttattt
     1441 ttgttgtctt aaagttgaag tttgccaata aattaaacag ttttctctcg ctggggatag
     1501 cgcccaaagg ggtatcaaat cgacaaaaat cgcgcgcaga aatcctcttt tcccctaaat
     1561 tgctttttgt tatagttaaa cagcggccaa ggggggcaag cgaaagcgca gaggccccgg
     1621 ggaacctctt tcctctcgct tttcccccga ctggtatctc ttaaattttg gtgacgaggt
     1681 gtgtttatgt ttgaattatt gcaagagacc tttacggatg aagacggaat ttctacccgc
     1741 tgcttcagtt tggtggaccg cggacgacag ttaaaaatcc cttatctgtg cacagaccgc
     1801 tgcaaggcag aaattttgca ggaacggctc aactccgagg cattgccccc ttttcaactg
     1861 caaaatttga tagaagatta tatccaaagt ctttatatgt aagttccata tcctatttgt
     1921 tttatatgat tttcctcttt cttatatgaa ttttgtcat
//
LOCUS       DBMDYA010000570         1949 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11892_length_1949_cov_1.452922, whole genome shotgun sequence.
ACCESSION   DBMDYA010000570 DBMDYA010000000
VERSION     DBMDYA010000570.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_1949_cov_1.452922"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(215..1300)
                     /locus_tag="ACZPFK_11990"
     CDS             complement(215..1300)
                     /locus_tag="ACZPFK_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1407071.1"
                     /translation="MANRVFQGVIYQMKDAVDRVLGVVDETGTIISCSELTKIGEVRD
                     GIAADRLSTGDVFVRDGYTYHMFSSNKRNDYAVFVEGTDDLAGRYASLITISLQNIKQ
                     YHDEKFDKANFIKNVVLDNILPGDIYAKARELHFSTDVSRVVLLIRVTSGNDISAYDV
                     VSGLFPDKQKDFVFNISENDTVLVKEIKRGIDREDIIKLAASIVDTLNGEHYIKSVIG
                     IGTPIANVKDLATSFKEAQIALEVSKVFDTEQTVVSYDNLGIARLIYQLPTTLCEMFL
                     KEVFKQGSIDSLDSETLFTIQRFFENNLNVSETSRGLFVHRNTLVYRLEKIKKLTGLD
                     LREFDDAIVFKVALMVKKYLNSNPAKY"
BASE COUNT          391 a          545 c          555 g          458 t
ORIGIN      
        1 ctttttgacg tttttgaatt ctatcattgg gtgaatctcc tttgcccgcg cgccgaaagt
       61 gaaaacgcag ccccgcaggg cggcggccgg cgggggctgc ccccgccgca tggccttgcg
      121 ccctgcggga ccctttgcat ccgcggcgcg ggccggcagc gtcacggccg ggcgcggatg
      181 cgttcgcccg tcgctttttc agcgcgcgcc cgctttaata ttttgcgggg ttggaattga
      241 ggtatttttt gaccatcagc gccaccttga agacgatggc gtcgtcgaac tcacgcaggt
      301 cgaggccggt gagctttttg atcttttcaa gccggtagac cagggtgttg cggtgcacga
      361 acagcccgcg gctggtctcg gaaacgttga ggttgttctc aaagaaccgc tggatggtga
      421 acagggtctc agagtccagc gagtcgatgc tgccctgctt gaagacctct ttgaggaaca
      481 tctcgcacag ggtggtgggc agctggtaga tcagccgggc aatgcccagg ttgtcgtagc
      541 tgaccacggt ctgctcggtg tcgaacacct tgctcacctc cagcgcgatc tgggcctctt
      601 taaagctggt ggccaggtcc ttgacgttgg cgatgggggt gccgatgccg atgacgcttt
      661 tgatgtagtg ctcgccgttg agggtgtcca cgatgctggc ggccagcttg atgatgtcct
      721 cgcggtcgat gccgcgcttg atctccttga ccagcacggt gtcgttttcg ctgatgttga
      781 acacaaaatc cttctgtttg tcggggaaca ggccgctcac cacgtcgtag gcggagatgt
      841 cgttgccgct ggtgacccgg atgagcagca ccacgcggga cacgtcggtg gagaagtgca
      901 gctcccgcgc cttggcgtag atatcgccgg gcaggatatt gtccagcacc acgtttttga
      961 tgaaattggc cttgtcgaac ttttcatcgt ggtactgctt gatgttctgc agcgagatgg
     1021 tgatcagcga cgcatagcgc cccgccagat cgtcggtgcc ctccacaaag acggcgtaat
     1081 cgttgcgctt gttggagctg aacatgtgat aggtgtagcc atcccgcaca aagacgtcgc
     1141 cggtcgacag ccggtcggcc gcgatgccgt cccgcacctc gccgatcttg gtcagttcgc
     1201 tgcaggagat gatggtgccg gtttcgtcca cgacgcccag gacgcggtca accgcgtcct
     1261 tcatctggta gatcacgccc tgaaacactc tgttcgccat aatgatcgat ttccttctct
     1321 ggcagtggct gccgattgtg aatttcacca atttgccgtg tagtatattt ctattttaca
     1381 caatgctttc gtcttttgca ataggaatcg acaaaataac cagccgcttc ctgtgcaagt
     1441 tgataaaatt ccctgcgcag cgggaaaccg cgctcaaaaa gcctccaaaa actggaaccg
     1501 acccgcccgc tttttgatac ctttgtataa cgttttgtca caaagccata aaagcagtcc
     1561 cgcgggccgc ccccggccca ccgcctgcgc gcgcccgcag ggggaaaggg gtaaaaacga
     1621 ttttatttcc atggtcccat ttctcgccgc tgcgcggcaa ccgaaacgga tggcccgaaa
     1681 aatagcctgc ggctattttt tatcgctccg cgataaaaaa caacttatct taatggcccc
     1741 ggtccaagga tggaccggat ggccgaaaaa tagcctgcgg ctatttttta tcgctccgcg
     1801 ataaaaaata acttatctta atggccccgg cccaaggatg ggccggatgg ccgaaaaata
     1861 gcctatggct attttttatc gctccgcgat aaaaaataac ttatcttaat ggccccggcc
     1921 caaggatggg ccggatggcc gaaaaatag
//
LOCUS       DBMDYA010000571         1949 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11894_length_1949_cov_1.108766, whole genome shotgun sequence.
ACCESSION   DBMDYA010000571 DBMDYA010000000
VERSION     DBMDYA010000571.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11894_length_1949_cov_1.108766"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..149)
                     /locus_tag="ACZPFK_11995"
     tRNA            complement(73..149)
                     /locus_tag="ACZPFK_11995"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Asp,seq:gtc)
     gene            complement(153..228)
                     /locus_tag="ACZPFK_12000"
     tRNA            complement(153..228)
                     /locus_tag="ACZPFK_12000"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Trp,seq:cca)
     gene            complement(409..1476)
                     /gene="mnmA"
                     /locus_tag="ACZPFK_12005"
     CDS             complement(409..1476)
                     /gene="mnmA"
                     /locus_tag="ACZPFK_12005"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1407072.1"
                     /translation="MRYSSGRVLVALSGGVDSTVCVQLLKDQGFSVEALVLRFSPAHE
                     KAVAAARQAAQQLDVPLTVRDCEPEFEREVIRPFCAEYCAGRTPNPCVICNPRVKFRL
                     LCAAADELGIPLVASGHYARIARQDGAFYVRRAVSTARDQSYMLYRLPQAILSRLLLP
                     VGEFEKREIRERAAALGLAAADAPDSQEICFIPGGDYASYIEGCGCRGPAGRFIAPDG
                     SVLGPHKGVLHYTVGQRRGLGVALGRPAFVKRILPGGDIELAFSGDEYYAAVQLTDLA
                     ETGVGLTPGGRYQVKIRSAAKPAPCTVAAREAHSALVVFDEPARAPAPGQSAVLYRGD
                     LLVGGGVISEILPQVSAGQRP"
     gene            complement(1497..1925)
                     /gene="nifU"
                     /locus_tag="ACZPFK_12010"
     CDS             complement(1497..1925)
                     /gene="nifU"
                     /locus_tag="ACZPFK_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR1407073.1"
                     /translation="MDHFSNPRNVGEIADADGVGEVGNPTCGDIMKMYLKIDGGVITD
                     VKFKTFGCGAAIATSSIATEMIKGRSLEEALKLSNKAVIEALDGLPPVKIHCSVLAEQ
                     AVKAALSDYYRRQGVDPEPIVGKLVEDCHACEAAGCGAPQ"
BASE COUNT          376 a          659 c          610 g          304 t
ORIGIN      
        1 gtctatgcga ttcgaacgca cgacctcttc cttaccaagg aagtgctctg ccgactgagc
       61 taagacagca aatggcgacc cggatgaggc tcgaactcac gacctctagc gtgacaggct
      121 agcgttctga ccaactgaac taccgggcca catggcaggg gcagaaggac ttgaaccctc
      181 ggcacgtggt tttggagacc actgctctac caactgagct atacccctat cttgggcagg
      241 cccttaagtg agggcgcttt aatataataa cgaaaacagt aaaaaaagtc aagccccccg
      301 cccaaaaatt ttgacagatt tttcagaggg cggcaaagcg ccgaaaaggc ggggccgagg
      361 cgtccgaaaa cgaaaggccc ggggccccag cggcgacgcg gccctgccct atggccgctg
      421 tccggcagag acctgcggca ggatctcgct gatcaccccg ccgcccacca gcaggtcgcc
      481 tcggtacagc acggcggact ggccgggggc cggtgcgcgg gcaggctcgt cgaaaacgac
      541 gagggcgctg tgcgcctccc gcgcggcgac cgtgcagggg gcgggctttg ccgcagagcg
      601 aatcttgacc tgatagcggc cgcccggggt cagcccgacg cccgtctcgg ccaggtcggt
      661 cagctgcacg gcggcgtagt actcgtcgcc ggaaaacgcc agctcgatgt ccccgccggg
      721 caggatgcgc ttgacaaagg ccgggcgccc cagcgccacc cccagcccgc ggcgctggcc
      781 caccgtgtag tgcagcacgc ccttgtgcgg ccccagcacc gagccgtcgg gcgcgataaa
      841 gcggccggcc gggccgcggc agccgcagcc ctcgatgtaa gacgcatagt cgccgccagg
      901 gataaagcag atctcctggc tgtccggcgc gtccgcggcg gcaagaccga gcgcggcggc
      961 cctctcccga atctcccgct tttcaaactc ccccaccggc agcagcagcc ggctcaggat
     1021 cgcttggggc aggcggtaga gcatatagct ctggtcccgc gccgtgctca ccgcgcgacg
     1081 cacataaaaa gccccatcct gccgggcgat acgggcgtag tgcccgctgg cgaccagcgg
     1141 gatgcccagt tcgtcggcgg cggcacagag cagccggaac ttgacccgcg ggttacagat
     1201 cacacagggg ttcggcgtgc gcccggcaca gtattccgca caaaaaggcc tgatgacctc
     1261 ccgctcgaac tccggctcgc agtcgcgcac cgtcaggggc acatccaact gctgcgcagc
     1321 ctgccgggcg gcggccacgg ctttttcatg cgcgggggaa aagcgaagga ccagggcctc
     1381 cacagaaaag ccctggtctt tgagcagctg gacgcagacg gtggaatcca cgccgcccga
     1441 cagggcgaca aggacgcggc cagacgaata gcgcataaaa acgatccctc cattttttac
     1501 tgcggcgcgc cgcagcccgc cgcctcacag gcatgacagt cttccaccag cttgccgacg
     1561 atgggctccg ggtccacgcc ctggcggcgg taatagtcgg acagcgcggc tttcaccgcc
     1621 tgttcggcca gcacgctgca gtggatcttg accgggggca ggccgtccag cgcctcgatc
     1681 accgccttgt tggagagctt gagggcctct tccaacgatc ggcccttgat catctcggtg
     1741 gcgatgctgg aggtggcgat ggccgccccg cagccaaagg ttttgaactt gacgtcggtg
     1801 atcacaccgc cgtcgatctt caggtacatc ttcatgatgt cgccgcaggt ggggttgccc
     1861 acctcaccga ccccgtcggc gtcggcgatc tcccccacat tgcgcgggtt ggaaaaatga
     1921 tccatcactt ttccaacccg cgcaatgtg
//
LOCUS       DBMDYA010000572         1946 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11911_length_1946_cov_1.570190, whole genome shotgun sequence.
ACCESSION   DBMDYA010000572 DBMDYA010000000
VERSION     DBMDYA010000572.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11911_length_1946_cov_1.570190"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            45..>1946
                     /gene="feoB"
                     /locus_tag="ACZPFK_12015"
     CDS             45..>1946
                     /gene="feoB"
                     /locus_tag="ACZPFK_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1407074.1"
                     /translation="MIFALAGNQNCGKTTLFNQLTGSNQHVGNFPGVTVEQKEGTVRK
                     EKDVYVVDLPGIYSLSPYTSEEIVTRDYLLSGKADGVINIVDATNIERNLYLSMQLIE
                     LGLPMVIALNMMDEVRANGTSIDIQKLSQELGVPILPISASKDEGVDELVKVAVETVR
                     QRRLPARLDFCSGAVHRAIHSIAHMVEDHAAAIGVPPRFAATKLVEGDEPMLARLKLS
                     DNEVDMLWHTVTEMEGEVGTDREAAMADMRYTFIEKLCADTVTKQGESKEHLRSIRID
                     AVLTHKYFAIPIFIGIMALVFWLTFGVVGALLSDLLDLGVQSLTALTEQALVSFGTNP
                     VLRSLIIDGVFAGVGSVLSFLPIIVVLFFFLSILEDSGYMARVAFVMDSLLRKIGLSG
                     RSFVPMLIGFGCSVPAIMATRTLSSERDHKMTILLIPFMSCSAKLPIYALFTAAFFPE
                     HGALVMILLYVVGMALAVLAGLIFKDTLFAGNPVPFVMELPNYRLPSPKSVALLIWDK
                     VKDFLTRAFTVIFVASIAIWVLQTFDIRFNIVADSADSMLAAVGRFIAPVFAPLGFGD
                     WRAATALISGFTMKEAVVSTFAVLVGGGNLNAALAGIFTPLAAFAFLIFTLLYTPCVA
                     AVGAVKRELN"
BASE COUNT          324 a          610 c          621 g          391 t
ORIGIN      
        1 catctgatgc gtcatgcgga ctcggctaaa gtgagggaga gcttatgatt tttgcactgg
       61 cgggaaacca gaactgcggc aagaccaccc tgttcaacca gctcaccggt tccaaccagc
      121 acgtgggcaa ttttccgggt gtgacggtgg aacaaaagga gggcacggtc cgcaaggaaa
      181 aagacgttta cgtggtggac ctgcccggca tctactcgct gtcgccctat accagcgagg
      241 agatcgtcac tcgcgactat ctgctgtccg gcaaggcgga cggggtgatc aacatcgtgg
      301 acgccaccaa catcgagcgc aatctctacc tctcgatgca gctcatcgag ctggggctgc
      361 cgatggtgat cgcgctgaac atgatggacg aggtgcgggc caacggcacc tccatcgaca
      421 tccaaaagct gtcgcaggag ctgggcgtgc ccatcctgcc catctccgcc tccaaggacg
      481 agggggtgga cgagctggtg aaagtggcgg tggaaacggt gcgtcagcgg cggctgccgg
      541 cgcggctgga cttctgctcc ggcgcggtgc accgggccat tcactccatc gcccacatgg
      601 tggaggacca cgccgcggcc atcggcgtgc cgccccgttt tgccgccacc aagctggtgg
      661 agggggacga gccgatgctg gcccggctga agctgtcgga caacgaggtg gatatgctct
      721 ggcataccgt caccgagatg gagggggagg tgggcaccga ccgcgaggcc gccatggcgg
      781 atatgcgcta tacctttatc gaaaagctct gcgccgacac ggtgaccaag cagggcgaga
      841 gcaaggagca tctgcgcagc atccgcatcg acgcggtgct cacccacaaa tacttcgcca
      901 tccccatctt catcggcatc atggcgctgg tgttctggct cacgttcggg gtggtggggg
      961 ctcttctcag cgacctgctg gacctggggg tgcaaagcct gaccgcgctg accgaacagg
     1021 cgctggtcag cttcggcacc aacccggtgc tgcgcagcct catcatcgac ggcgtctttg
     1081 cgggcgtggg ctcggtgctc agctttttgc ccatcatcgt ggtgctgttc ttttttctct
     1141 cgattctgga ggattcgggc tatatggccc gggtggcctt tgtcatggac agcctgctgc
     1201 gcaagatcgg cctgtcgggc cgcagctttg tgcccatgct gatcggcttc ggctgcagcg
     1261 tgccggccat catggcgacg cgcaccctct ccagcgagcg ggaccacaag atgaccattc
     1321 tgctgatccc gttcatgagc tgcagcgcca agctgcccat ctacgcgctg ttcacggccg
     1381 cctttttccc cgagcacggg gcgctggtga tgatcctgct gtacgtggtg ggcatggcgc
     1441 tggccgtgct ggcggggctg atcttcaagg acaccctctt tgccggcaac ccggtgcctt
     1501 tcgtgatgga gctgcccaac taccgcctgc ccagccccaa gagcgtggcc cttttgatct
     1561 gggacaaggt gaaagacttt ctcacccggg cgttcacggt catctttgtg gcctccatcg
     1621 ccatctgggt gctgcagacc tttgacatcc gcttcaacat cgtggcggac agcgccgatt
     1681 ccatgctggc ggcggtgggc cgcttcatcg ccccggtctt tgcgccgctg ggcttcggcg
     1741 actggcgcgc cgccacggcg ctgatctccg gcttcaccat gaaagaggcg gtggtctcca
     1801 cctttgccgt gctggtgggc ggcggcaacc tgaacgcggc gctggccggc atcttcaccc
     1861 cgctggccgc ctttgccttt ttgatcttca cgctgctgta caccccctgt gtggcggcag
     1921 tcggcgcggt caagcgggag ctgaac
//
LOCUS       DBMDYA010000573         1944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11926_length_1944_cov_2.019533, whole genome shotgun sequence.
ACCESSION   DBMDYA010000573 DBMDYA010000000
VERSION     DBMDYA010000573.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11926_length_1944_cov_2.019533"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="ACZPFK_12020"
     CDS             complement(<1..106)
                     /locus_tag="ACZPFK_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR1407075.1"
                     /translation="MAEKQNSAQGLHIGFIMDGNGRWAQKRGLPRTAGH"
     gene            complement(224..778)
                     /gene="frr"
                     /locus_tag="ACZPFK_12025"
     CDS             complement(224..778)
                     /gene="frr"
                     /locus_tag="ACZPFK_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1407076.1"
                     /translation="MSEMTQKYEAKMEGAVDHLFKELQGIRAGRANPGILDKVTVDYY
                     GTPTPINQVAAVAVAEARILTIQPWDASMMHAIEKAILTSDIGINPTNDGRLMRLVFP
                     APTEERRKALTKEAQKMAEEAKIAVRNVRRDAMDKFKAKKKAGELTEDDQKQLEEEMQ
                     KITDKFIKKVDKVCEDKIKEIMEI"
     gene            complement(780..1493)
                     /gene="pyrH"
                     /locus_tag="ACZPFK_12030"
     CDS             complement(780..1493)
                     /gene="pyrH"
                     /locus_tag="ACZPFK_12030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1407077.1"
                     /translation="MELKYKRILLKLSGEALAGDQKMGIDYNVVLNICKSIQRAQALG
                     AEIAIVVGGGNFWRGRSSGNMERTRADQIGMLATVMNALAVADALEHLGLEVRVQTAI
                     SMQQIAEPYIRNRATRHLEKGRVVILACGTGNPFFSTDTASALRAAEINAEIVFKATM
                     VDGVYDKDPHRFDDAKRYHTLTFSQVLADQLAVMDSTAATMCRDNGLPLLVFDISDPE
                     NIVRALCGEDVGTLVKEDE"
     gene            complement(1688..>1944)
                     /locus_tag="ACZPFK_12035"
     CDS             complement(1688..>1944)
                     /locus_tag="ACZPFK_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407078.1"
                     /translation="MRLMWVTLGTNIASLLLCIAIFADGSIFNRHLVQDLAAAGVQLS
                     EGAWAVEHFGTFLLALLIFAFVLDIATDLWRTLRYRDTVQ"
BASE COUNT          321 a          639 c          568 g          416 t
ORIGIN      
        1 tgtgccccgc cgtgcgcggc aggccgcgct tttgggccca gcggccgttg ccgtccatga
       61 taaaaccgat gtgcagtccc tgtgcgctgt tctgtttctc cgccatctcg ccctcccggt
      121 cgtccctgaa aaggccgagc cgcagctcgg ccggagggga agcgctgtcc ccagccctcc
      181 ccaccagccc gcgcggcccg gcgtcctgcc ccgtccacaa cggtcagatc tccatgatct
      241 ccttgatctt gtcctcgcag accttgtcga cttttttgat gaacttatcg gtgatcttct
      301 gcatttcctc ctcgagctgc ttttgatcgt cctcggtcag ctcgccggct ttctttttgg
      361 ccttgaattt gtccatggcg tcgcggcgca cgttgcgcac cgcgatcttc gcctcctcgg
      421 ccatcttctg cgcctctttg gtgagggcct tgcggcgctc ctccgtgggg gccgggaaga
      481 ccagccgcat caggcggccg tcgttggtgg ggttgatgcc gatatcgctg gtgaggatcg
      541 ccttttcgat ggcgtgcatc atcgaggcgt cccagggctg gatggtgagg atacgggcct
      601 cggccaccgc caccgcggcc acctggttga tgggggtcgg ggtgccgtag taatcgacgg
      661 tcaccttgtc caggatgccc gggttcgcgc ggcccgcgcg gatgccctgc agctccttga
      721 agagatggtc gacggcgccc tccattttcg cttcgtactt ctgggtcatc tcgctcatgt
      781 cattcatcct ccttgactaa tgtgccaacg tcctcgccgc agagcgcgcg gacaatattt
      841 tccgggtcgg aaatgtcgaa caccaacagc ggcaggccgt tatcgcggca catggtcgca
      901 gcggtggaat ccatcaccgc cagctggtcc gccagcacct gcgaaaaggt gagggtgtgg
      961 tagcgcttcg cgtcgtcaaa gcggtgcggg tccttgtcgt agacgccgtc caccatcgtc
     1021 gccttaaaga cgatctccgc gttgatctcc gcggcgcgca gcgctgaggc ggtatcggtg
     1081 gagaagaacg ggttgccggt gccgcaggcc aggatcacca cccggccctt ttccagatgc
     1141 cgggtggccc ggttgcggat atagggctcc gcgatctgct gcatgctgat agcggtctgc
     1201 acccgcacct ccaggcccag gtgctccagc gcgtcggcca ccgccagggc gttcatcacg
     1261 gtggccagca tgccgatctg gtcggcgcgg gtgcgctcca tattgccgct ggagcggccg
     1321 cgccagaagt tgccgccgcc cactacgatc gcgatctcag cgcccagggc ctgcgctctc
     1381 tggatgctct tgcagatgtt cagcaccaca ttatagtcaa tgcccatttt ctggtcgccc
     1441 gccagcgcct cgccggaaag cttcaacagg atccgcttat atttcagttc caaaaataaa
     1501 ccaccgccca gttttttctc ttttgcgccg ccgtgacggg cccggcccgt ttttcacccg
     1561 gcggtcattg ctcactctat tttacaatag ttggagtttc ttgtaaagcg caatcaccgc
     1621 ccaaaaagaa aacagacacg gaaatttcca tgtctgttct gtgaactgaa cgccgtgttg
     1681 ccgcctctca ctgcacggta tcccgatagc gcagcgtgcg ccagagatcg gtcgcgatat
     1741 ccagcacaaa ggcgaagatc agcaacgcca gcagaaaagt gccgaaatgc tccaccgccc
     1801 aggcgccctc gctcagctgt acgcccgccg cggccaagtc ctgcaccagg tgccggttga
     1861 agatgctgcc gtcggcgaag atggcgatgc acagcagcag cgaagcgata ttcgtgccca
     1921 gcgtgaccca catcaggcgc atgg
//
LOCUS       DBMDYA010000574         1940 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11955_length_1940_cov_1.727026, whole genome shotgun sequence.
ACCESSION   DBMDYA010000574 DBMDYA010000000
VERSION     DBMDYA010000574.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11955_length_1940_cov_1.727026"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="ACZPFK_12040"
     CDS             <1..932
                     /locus_tag="ACZPFK_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR1407079.1"
                     /translation="AHVFDDLDACVTWHPSSYTAPWANSMLANLSVRFRFKGVPAHAA
                     SAAELGRSALDAVELMNVGANYLREHVNEKVRMHYVTTNGGSVPNTVPEDAEVWYMVR
                     APKFAMVRDTFRRLKGIARGAAQMTETELAEVRLESAAWEVLTNEVICGVIDRNMKAM
                     GGPGFAEEDRGFAAEMVKTFGPGEQVKGFAPFFVPAELEGQGLFEGVADPVGRGVCIM
                     ASSDVGDVSWITPLCQFGAAVWPLGIPTHTWRTTASTSSHLAMRGMLFAAKTMAATLY
                     DLCADPALLKEAQTEFSRATEKQKYVCALDEEI"
     gene            complement(1453..>1940)
                     /locus_tag="ACZPFK_12045"
     CDS             complement(1453..>1940)
                     /locus_tag="ACZPFK_12045"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1407080.1"
                     /translation="WLNHMAAQGLALVAVGFCRYTFEECLPGEYAVRLELMEQLPESA
                     EGQQYLRFIEETGAEYLGAIARWAYFRKRTERGGFDLFSDLDSRLAHLKRILVLFIPF
                     IFLNLWPACWNLSLFLQNRFWLSLFCSVLGAVMVALLTYGFFRIWRKRRRLLQERSLH
                     E"
BASE COUNT          361 a          600 c          652 g          327 t
ORIGIN      
        1 aagcccacgt ctttgacgac ctggacgcct gtgtcacctg gcacccgtcc tcttataccg
       61 ccccctgggc caacagtatg ctggcgaacc tgtcggtgcg ctttcgcttc aagggcgtcc
      121 cggcccacgc ggcctcggcg gcggagctgg gccgcagcgc gctggacgcg gtggagctga
      181 tgaacgtggg ggccaactac ctgcgcgagc acgtaaacga aaaggtgcgc atgcactatg
      241 tcaccaccaa cggcggcagt gtgcccaaca cggtgcccga ggacgccgag gtctggtata
      301 tggtgcgggc gcccaagttt gcgatggtgc gggacacatt ccggcggctc aagggcatcg
      361 cgcggggcgc cgcccagatg accgagaccg agctggctga ggtgcggctg gaatccgcgg
      421 cctgggaggt gctcaccaat gaggtgatct gcggtgtgat cgaccgcaat atgaaagcca
      481 tgggcggccc gggctttgca gaggaggacc gcggctttgc cgcggagatg gtgaagacct
      541 ttggtcccgg cgagcaggta aaagggttcg cgcctttctt cgtgcctgca gagttggagg
      601 ggcagggcct gttcgagggg gtggcggacc cggtgggccg cggcgtctgc atcatggcct
      661 ccagcgacgt gggagacgtg agctggatca cgccgctgtg ccagttcggc gccgcggtct
      721 ggcccctggg catccccacc cacacctggc gcaccacggc ctccaccagc agccatctgg
      781 ccatgcgggg catgctcttt gccgccaaga ctatggcagc caccctgtac gacctctgcg
      841 ccgacccggc gctgctgaaa gaggcacaaa cggagttcag ccgtgcgacg gaaaagcaaa
      901 agtacgtctg cgcgctggac gaggagatct gagcatccat cggcattggg acaaaaaggc
      961 cgtcgacagc gattgtttgt ccccccgggc gcttggcgcc cggggggctt cgttttaaaa
     1021 tcacagactg tggcggacgg tacacgggcc gccttttgcg acggtcattc cctttcagcc
     1081 gttgccgttg agaccggggc tgctccggct ctgctcaggc acctcagctt ctgcggatga
     1141 cggcccaaag cggaggaccg tgcacctggc cctgggatga gcaaaaagaa aagcccgctg
     1201 cgcagagcgg gaaacggcgg tccgcagggc gtcggagacg cgcagggcaa gcctcccgtg
     1261 ctgcaggctc ccctgcgtca gctggccgac gggcggcgga gcctttttgg tgagcgcttt
     1321 ggaacgggga ttttttaagg acggcctcgg gactttcttg cctcctctgc gcttgccgaa
     1381 gaaaaggaac ggctgtcgcc tggcgacagc cgttcctttt cttttctgtt ccgtgacgca
     1441 tccgggcgcg cattattcgt gcagcgaccg ctcctgcagc aggcggcggc gcttgcgcca
     1501 gatcctgaaa aagccgtaag tgagcagcgc gaccatcacc gcccccagga cactgcagaa
     1561 caagctgagc cagaaccggt tctggagaaa gagcgagagg ttccagcagg cgggccagag
     1621 attgagaaaa atgaacggga tgaaaaggac gagaatgcgc ttgagatggg caaggcggga
     1681 gtcgagatcc gagaacagat caaagccgcc gcgttccgtc cgcttgcgga aataggccca
     1741 gcgggcgatg gcgcccagat actcggcgcc ggtctcctcg atgaagcgca ggtactgctg
     1801 cccctcggcg ctctcgggca attgctccat cagctcgagc cgcaccgcgt attctccggg
     1861 caggcactcc tcgaacgtgt agcggcaaaa ccccaccgcc accagcgcca ggccctgggc
     1921 ggccatgtgg ttcagccact
//
LOCUS       DBMDYA010000575         1933 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_11994_length_1933_cov_1.370087, whole genome shotgun sequence.
ACCESSION   DBMDYA010000575 DBMDYA010000000
VERSION     DBMDYA010000575.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11994_length_1933_cov_1.370087"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="ACZPFK_12050"
     CDS             complement(<1..194)
                     /locus_tag="ACZPFK_12050"
                     /inference="COORDINATES: protein motif:HMM:NF027405.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1407081.1"
                     /translation="MELNGFGIAGVACITVICYLAALGVKQTKLANKWLPTICGALGA
                     VLGPVCMLVVPDFPAGDPVTA"
     gene            complement(267..1349)
                     /locus_tag="ACZPFK_12055"
     CDS             complement(267..1349)
                     /locus_tag="ACZPFK_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1407082.1"
                     /translation="MKNGIDVSKYQNAIDWPRVAASGVHFAFVRVGWAGYEGGIDEGL
                     DPCFARNMAGAAAAGLAVGAYVYSYCKTPAAARRAAREAATLLAPYRLTMPLAFDIED
                     AATYKALGRAQSSAVAAAFLEEAKAQGYYPLLYTYTSFAQSYLDMSALSAYDLWLADY
                     RGYMGLKGASIWQHTSDGWVDGISGRVDLNIAYKDYPALIGRQGGQKEDKDDMMQFLE
                     VFGSKNCQCFTGPDVNAVDRSHNNGTLASGTYYPLMADCGLGADGYRWVRVLAGGAER
                     YAVVLDDRCRITCLSAGDAVRAVQAQGAGGDAAALARRVKELESSNAGLLARASAAEA
                     QSAEASRRAAEYLKRIKAAAAALEVL"
     gene            complement(1409..>1933)
                     /locus_tag="ACZPFK_12060"
     CDS             complement(1409..>1933)
                     /locus_tag="ACZPFK_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1407083.1"
                     /translation="TGVRRGELCGFLKSDFAPRRHLYQVARAVRRGQAGPELGEPKAH
                     SRRTNPLSPHAQQAYDLLCARYPDSPYLLPGPDGGLMRPDTWSRRLKAEMERLTAAHP
                     DLPQLTAHELRHTYGTSLRRHGADIYSIAKILGHRDVEVTARIYVHNELDELKRATRY
                     TRRQQPLPVQPSAA"
BASE COUNT          284 a          671 c          626 g          352 t
ORIGIN      
        1 gcggtgaccg ggtcgccggc cgggaaatcc ggcaccacca gcatgcacac cggccccagc
       61 accgccccca gcgccccgca gatcgtgggc agccacttgt ttgcaagctt tgtctgcttc
      121 actcccagcg ccgccagata gcagatcacc gtgatgcagg ccacgcctgc aatgccaaac
      181 ccgttcagtt ccatttactt ttcctccttg atgtttttcc ttgatatcgt cgatctgcat
      241 tcagaagctc atgtcaaact gcggcatcac agcacctcca gcgcggcggc ggccgccttg
      301 atccttttga gatactccgc cgcccggcgg gacgcttcgg cgctctgcgc ctctgccgcc
      361 gaggcccggg ccagcaggcc ggcgttgctg ctctccagct ctttgacccg ccgggccaga
      421 gccgccgcgt cgccgcccgc gccctgggcc tgcaccgccc gcaccgcgtc cccggcggaa
      481 aggcaggtga tcctgcagcg gtcgtccagc accacggcgt agcgctccgc gcccccggcc
      541 agcacccgca cccagcggta gccgtcggct ccgaggccgc agtcggccat gagggggtaa
      601 tatgtaccgc tcgccagcgt gccgttgttg tgggacctgt ccaccgcgtt gacgtccggc
      661 ccagtgaagc actgacagtt tttgctgccg aacacctcca gaaattgcat catgtcgtcc
      721 ttgtcctcct tttgtccgcc ctggcggccg atcagggcgg ggtaatcctt gtaggcgatg
      781 ttgagatcga cccggcccga gatgccgtcc acccagccgt cgctggtgtg ctgccagatg
      841 ctcgcgcctt tgagccccat ataccctcgg taatccgcca gccagaggtc gtaggccgac
      901 agggcgctca tgtcgagata gctctgtgcg aagctggtgt aggtgtagag cagaggataa
      961 tacccttgcg cctttgcctc ttccaaaaag gccgccgcca cggcgctgct ctgggcccgg
     1021 cccagagcct tgtaggtggc cgcgtcctcg atgtcaaagg ccagcggcat ggtcaatcgg
     1081 tagggggcca gcagggtcgc ggcctcccgg gccgccctgc gggcggcggc gggcgtcttg
     1141 caatagctgt agacgtaggc ccccacggca agccccgcgg cggccgcgcc cgccatgttg
     1201 cgggcaaagc aggggtccag cccctcgtcg atacctccct catacccggc ccagccaacc
     1261 cgcacaaagg cgaagtggac gcccgatgcc gccaccctgg gccagtcgat ggcgttttgg
     1321 tattttgaga cgtcgatgcc gtttttcact ttttacctcc ttgtagggtg ttttattgta
     1381 aacagtcgct ggcgagcggc ggcagaggct acgcggcgga gggctggacc gggagcggct
     1441 gctgacggcg cgtgtagcgc gtcgctcgct tgagctcgtc cagctcgtta tgcacgtaga
     1501 ttctcgccgt cacctcaacg tcccggtgac cgagtatctt tgcgatgctg tagatgtccg
     1561 cgccgtggcg gcgcaggctg gtgccgtagg tgtgccgcag ctcgtgggcc gtgagctggg
     1621 gcaggtccgg gtgggcggcc gtcagccgct ccatctccgc cttgaggcgg cgggaccagg
     1681 tgtccggccg catgaggccg ccgtccggcc cgggcagcag gtaggggctg tccgggtagc
     1741 gggcgcagag caggtcatag gcctgctgcg cgtgcgggct cagcgggttc gtgcggcggc
     1801 tgtgcgcctt gggctcgccc agctccggcc cggcttgccc ccggcgcacc gcccgggcca
     1861 cctggtaaag atgccgccgc ggggcaaaat cagacttgag aaacccgcac agctcgcccc
     1921 ggcgcacccc ggt
//
LOCUS       DBMDYA010000576         1930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12009_length_1930_cov_1.622745, whole genome shotgun sequence.
ACCESSION   DBMDYA010000576 DBMDYA010000000
VERSION     DBMDYA010000576.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_1930_cov_1.622745"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            166..>1930
                     /locus_tag="ACZPFK_12065"
     CDS             166..>1930
                     /locus_tag="ACZPFK_12065"
                     /inference="COORDINATES: protein motif:HMM:NF045973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIR1407084.1"
                     /translation="MKRPDRTEAALWAALMLPVLWLAAAVAQAIDMGGGLAGLAAHLS
                     ALIAAPFSVHWSASTGKFLLLALICYPLAVFCYLEEKSRQHPGAEHGTARWGSAFSLN
                     RKYRDRKRPSENYIFTSHVQMGMDGFRHKHNLNVVVIGGSGAGKTRFYVKPNLMQCAE
                     NCSYIILDSKGEILRSVGGLFERMGIAVTVVDLVHFAGHYNPMAYLETDEDAIKLAYA
                     LVHNTKPKDAPAGGDKFWDDSSMMLISAMLLYLMYEAPPYEQNFAMLMHMILNGPVAE
                     GEAGPNPLDLLFEGLEGREPDHPAVLQYHSFKLGSTRTLQSVLISAAANLYMFNTRRF
                     AEMTSQDETFLPRLGLEHRAIFCVIPDHDQTYNFLVTILYTQLFDQLFRLADSNPDYH
                     GALPVHVRLMMDEFSNVALPSNFQNILSVCRSRNISCDIILQNIAQIKKLFKDDWEGL
                     LGNCDTMLYLGGNEYGTFEYLSKILGKETERTTSQSIGKGSRGSSSESRQTAGRELLT
                     PDEVRRIERGDALLLMSSEDPVIDRKFDLLRHPNVRYTPDGGSEPYVLPPDYMSEATT
                     LSAAELATLEPDKVPAEILDQY"
BASE COUNT          419 a          552 c          576 g          383 t
ORIGIN      
        1 aaatgggcca ggtgctttct gagcaggcgc aacgtccgtc tgtgcgggct actctcgcgg
       61 cattaaaagc aatcgcccaa aagacggcac aggcccagga acaggcaatc gaaaccgcag
      121 caagggaggg acgcgagtga acggcacttc aatgcggggt aacccgtgaa acgccccgac
      181 cgcacagaag cggccctctg ggcggcgctg atgctgccgg tactctggct ggcagcggcg
      241 gtggcacagg caatcgacat gggcggcggg cttgcagggc tcgccgccca tctctctgcc
      301 ctgatcgccg cgcccttctc ggtgcattgg agcgccagca ccggcaaatt tttgctgctg
      361 gcattgatct gctacccgct ggccgtgttt tgctatttag aggaaaaatc ccggcagcat
      421 cccggcgcag agcatggcac ggcacgttgg ggcagtgcgt tctcgctcaa ccggaaatac
      481 cgtgaccgca aacgcccatc cgaaaactat atttttactt ctcatgtgca gatgggcatg
      541 gatggctttc ggcacaagca caatctcaat gtggtcgtca tcggcggcag cggcgcagga
      601 aagacgagat tctacgttaa gcctaatctg atgcaatgcg cggagaactg ctcgtacatc
      661 atcctcgaca gcaagggcga gatcctgcgc agcgtgggcg gcctgtttga gcggatgggc
      721 atcgccgtca cggtcgttga ccttgtgcat ttcgccgggc attataaccc gatggcttac
      781 cttgaaacag acgaggacgc gatcaagctg gcctatgcgt tggtgcataa caccaaaccc
      841 aaggatgcgc cagcaggcgg ggataagttt tgggatgatt cctcgatgat gctgatctcg
      901 gccatgctgc tctatctgat gtatgaggcc ccgccatacg agcagaattt tgcgatgctg
      961 atgcacatga tcctcaacgg cccggtagcc gagggggagg ccgggccaaa cccgctcgat
     1021 cttctgtttg agggactgga gggtcgggaa cccgatcacc ccgccgtgct gcagtatcac
     1081 tcgttcaagc tgggcagcac caggacgctg caatcggttc tgatttcggc ggcggcaaac
     1141 ctgtatatgt tcaacacgcg ccgctttgcc gagatgacca gccaggacga gacctttctg
     1201 ccgcggctgg ggctggagca ccgcgcgatc ttctgcgtga tccccgacca tgaccagacc
     1261 tacaacttcc tcgtcaccat cctgtacacg cagctctttg accagttgtt tcggctggcc
     1321 gattcaaacc cggattatca cggcgcgctg ccggttcatg tccgcctgat gatggacgag
     1381 ttcagcaacg tggccctgcc cagcaacttc caaaacatcc tctcggtctg ccgcagccgg
     1441 aatatcagct gcgatattat cctgcaaaac atcgcacaga tcaaaaagct gttcaaggac
     1501 gattgggagg ggctgctcgg aaactgcgat actatgctgt atttgggtgg gaatgagtac
     1561 gggacatttg agtatttatc aaagattttg ggcaaagaga ccgagcgcac cacctcgcag
     1621 agcatcggca agggcagccg tggctcgtcg agcgagagcc gccagacagc gggccgagag
     1681 cttttaaccc cggacgaggt gcggcgcatc gagcgcgggg acgcgctgtt gttaatgagc
     1741 agcgaggacc cggtgattga tcggaagttt gatctgctcc gccacccgaa tgtccgttac
     1801 accccggacg gcggcagtga gccgtatgtg ctgccgcccg attacatgag cgaagcgacc
     1861 accctatccg cagcagaact ggcaacgttg gagccggaca aggttccggc agaaattttg
     1921 gatcaatatg
//
LOCUS       DBMDYA010000577         1918 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12099_length_1918_cov_1.364887, whole genome shotgun sequence.
ACCESSION   DBMDYA010000577 DBMDYA010000000
VERSION     DBMDYA010000577.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12099_length_1918_cov_1.364887"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(4..1701)
                     /locus_tag="ACZPFK_12070"
     CDS             complement(4..1701)
                     /locus_tag="ACZPFK_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1407085.1"
                     /translation="MSYEKYCAGCHGTFPAGESVCPACGAECPNDNPGGTLPVGLFLA
                     ERYVIGRVLEVDGEGITYEAIDQDSGLPVIIKEYLPVTLCSKRDETGAVVVKAGSEVP
                     YKTTLVDFVDLYKNLMQLAGRQGLARVEALFKANNTVYAVCEYQKSISLTDWLNRQTE
                     LLSFERCYGLLAPVFAGLTALHSAGVVHRGVCPDNIRITPDGRAFLTGYATLALRTLN
                     SELKPCLYEGYSAPEQYSTTEFQGPYTDVYSLAAVLYKMLAGTAPVATAGRVDGCPTL
                     KQIGAEVSSQVSRAVDHAMWGDVNQRTQSVAELAQELAGDAKAAGLGSTRVMKAAPTQ
                     RPGRGGMGGNKGLIVGGCIALGLVVVFLIWMLIRGLVPRANSSSSLSSSSAASASASA
                     TPEPSQAPAQTVPNFVGQLYSDLQGNAEYQQKYIFNVTEQYSDNVEKGKVISQAPLSG
                     EDLPPDHKVDLVVSKGKQSVTIPEYRNYTRGDVVNVLTGAGIKAENIKFAEIANDGTM
                     NPGESVKGDREVGSTMNPETDIITVYMAGPLPTPTPEPTPTPTPEKIPVATPEPQQE"
BASE COUNT          308 a          629 c          611 g          370 t
ORIGIN      
        1 gggtcactcc tgctgaggct ccggcgtggc gacggggatc ttctccggcg tcggtgtggg
       61 cgtaggctcc ggcgtggggg tgggcagcgg ccccgccata taaacggtga tgatgtccgt
      121 ctccggattc atcgtggacc ccacctcccg gtcgcctttc accgactcgc ccgggttcat
      181 ggtgccgtcg ttggcgatct cggcaaattt gatgttctcc gccttgatcc ccgcgccggt
      241 gagcacgttg accacgtcgc cgcgcgtgta attgcgatat tcggggatgg tcaccgactg
      301 cttgcccttg ctgaccacca ggtccacctt gtggtcgggg ggcagatcct cgccggagag
      361 cggcgcctgg ctgatgacct tgcccttttc cacattgtcg gagtactgct cggtcacatt
      421 aaagatatat ttttgctggt actcggcgtt gccctgcagg tcgctgtaga gctggcccac
      481 aaagttgggc accgtctgcg ccggcgcctg cgagggctcc ggcgtggcgg aggcggaagc
      541 cgaggccgcg gaggagcttg agaggctgga ggaggaattc gcccgcggca ccaggccgcg
      601 gatcagcatc cagataagga ataccaccac caggcccagc gcgatgcagc cgccgacgat
      661 caggcccttg ttgccgccca tcccgccgcg gccggggcgc tgcgtgggcg cggctttcat
      721 cacccgcgtg gaccccaggc cggcggcctt ggcgtccccg gccagctcct gcgcgagctc
      781 tgccaccgac tgggtgcgct ggttcacatc gccccacatg gcatggtcca cagcgcggga
      841 gacctgtgag gacacctccg cgccgatctg cttcagggtg gggcagccgt ccacccggcc
      901 cgcagtggcc accggcgcgg tgcccgccag catcttatac agtaccgcag ccagcgagta
      961 gacgtccgta tacggcccct gaaactcggt ggtggaatac tgctctgggg cagaatagcc
     1021 ctcatagagg cagggcttca gctctgagtt gagggtgcgc agcgccaggg tggcatagcc
     1081 ggtcaaaaag gcgcggccat ccggcgtgat gcggatattg tccggacaca cgccccggtg
     1141 cacgaccccc gcgctgtgca gggccgtcag cccggcgaac accggggcca acaggccata
     1201 gcagcgctca aaggagagca gctcggtctg gcggttgagc cagtcggtga gggagatgct
     1261 cttctgatat tcacagaccg catagacggt gttgttcgcc ttgaacagcg cctctacccg
     1321 ggcaaggccc tggcgtccgg ccagctgcat caggtttttg tacaggtcga caaagtcgac
     1381 cagcgtggtc ttgtagggca cctcgctgcc ggctttcacc accaccgcgc cggtttcatc
     1441 ccgcttggag cagagggtga ccggcaggta ttccttaatg atcaccggca ggccggagtc
     1501 ctggtcgatg gcctcgtagg tgatgccctc cccgtcgacc tccagcaccc ggccgatcac
     1561 atagcgctct gccagaaaca ggcccacggg cagggtgccg cccgggttgt cgttggggca
     1621 ctcggcgccg caggccgggc agacgctctc ccccgccggg aaagtgccgt gacagccggc
     1681 gcagtatttt tcgtagctca taacttttca gccctttctt tgcacagggt ttctcttttt
     1741 cctcatccac catctgtgaa agcacacacc cgccgacttt ggcgccggtt cagcggtcca
     1801 ggtcctcttc gttctccagg ttgtgccaga agttctgcac atcctcgtcc tcttccaaaa
     1861 agtccagcag ctggcccatc cgcttgagat cctcctccgc ggagagggcg acggtggt
//
LOCUS       DBMDYA010000578         1914 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12136_length_1914_cov_1.119140, whole genome shotgun sequence.
ACCESSION   DBMDYA010000578 DBMDYA010000000
VERSION     DBMDYA010000578.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12136_length_1914_cov_1.119140"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="ACZPFK_12075"
     CDS             <1..516
                     /locus_tag="ACZPFK_12075"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1407086.1"
                     /translation="LFVKPVRAGSSFGITRVAAPEGLADAVALALRHDGQVLLEAAVR
                     GFEVGCAVLGNHSLTIGGPDEIELADGFFDFTEKYTLKTSRIHLPARIPEDKARQIRE
                     AAATVYRALGCRGFARVDLFLTPAGEIVFNEVNTIPGFTEHSRFPGMMKQAGLSFPEV
                     VNTAVRLAMEP"
     gene            513..1274
                     /locus_tag="ACZPFK_12080"
     CDS             513..1274
                     /locus_tag="ACZPFK_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1407087.1"
                     /translation="MKQSIALPSPARVLPRGELRLVNRSHPLPAELAPPALAPALPGH
                     PDILLEARAAAALTALVSALGRPGEIVPVSGFRPHWEQQQIWDDSLRDKGPDFTAKYV
                     AWPGCSEHETGLAIDLGFNRPPIDFIRPAFPRKGICQRFREQAAAYGFIERYRAGKEQ
                     VTGIGAEPWHFRFVGAPHAALMEARGLALEEYIQLLEDCAAKGEHLYHEDARGRWELW
                     PVPELPSVFTALPRGTALFYTGAGFLVAALRRAGR"
     gene            1271..>1914
                     /locus_tag="ACZPFK_12085"
     CDS             1271..>1914
                     /locus_tag="ACZPFK_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1407088.1"
                     /translation="MRETHTVFRAGAAPARPGTQGTRAGYPELDRFRLVAALLVVAIH
                     TSPLLSLGETADFLLTRVAARVAVPFFLMTSGFFLLPGLPAHGGFTNRQRQALGRFFR
                     RTALLWGAALLLYLPLNLYGGKLSANAPGEPLRYFLLEGGFYHLWYLPAALLGMALCL
                     ALLRLGRTAALGAAGALYLVGVFGDSWWGVAAKLPPLRSFYDGLLGFFGETRNGL"
BASE COUNT          230 a          677 c          658 g          349 t
ORIGIN      
        1 ctctttgtca agccggtgcg ggccgggtcc tctttcggca tcacccgcgt tgccgcgccc
       61 gaggggctgg ccgatgccgt ggcgctggcg ctgcggcacg acgggcaggt cctgctggag
      121 gctgcggtgc gcggctttga ggtgggctgc gcggtgctgg gcaaccacag cctgaccatc
      181 ggcggccccg atgagatcga actcgccgat ggattttttg atttcacgga aaaatacacc
      241 ctcaagacct cccgcatcca tctgcccgcc cgcatccccg aggacaaggc gcggcagatc
      301 cgcgaggctg cggccacggt ctaccgcgcc ctgggctgcc gcggctttgc gcgggtggac
      361 ctgttcctca ccccggcggg ggagatcgtc tttaatgagg tgaacaccat acccgggttc
      421 accgagcaca gccgttttcc cggcatgatg aagcaggcgg ggctctcttt ccccgaagtc
      481 gtaaatacgg cggtcaggct ggcgatggag ccgtgaaaca gagtatcgcc ctcccctcgc
      541 ccgcacgtgt gctgccgcgg ggcgagctgc ggctggtgaa ccgcagccat ccgctgcccg
      601 cagagctcgc gccgcccgcc ctcgcccccg cgctgccagg gcatccggac atcctgctgg
      661 aggcccgcgc cgcggccgcg ctcaccgcgc tggtgagcgc gctgggccgc cccggcgaga
      721 tcgtgccggt gagcggcttt cgtccccact gggagcagca gcagatctgg gacgactccc
      781 tgcgggacaa ggggccggat ttcacggcca agtatgtggc ctggcccggc tgcagcgagc
      841 acgagaccgg ccttgccatt gatctcggct tcaaccggcc tcccattgat ttcatccgcc
      901 ccgcctttcc ccgtaagggg atctgccagc gcttccgcga gcaggccgcc gcttacggct
      961 ttatcgagcg gtaccgggcg ggcaaggagc aggtcaccgg catcggcgcc gagccctggc
     1021 acttccgctt tgtgggcgcg ccccacgccg ccctgatgga ggcgcggggg ctggcgctgg
     1081 aggagtatat ccagctgctg gaggactgcg ccgccaaggg cgagcacctg tatcacgagg
     1141 acgcccgcgg ccgctgggag ctttggcccg tgcccgagct gccctccgtc tttaccgccc
     1201 tgccccgggg caccgccctc ttttacaccg gcgccggctt tctggtggcg gcgctgcgga
     1261 gggcgggccg atgagggaga cgcacactgt ttttcgcgcc ggggcggccc ctgcccggcc
     1321 cgggacccag gggacgcgcg cgggctatcc cgagctggat cgcttccgtc tcgttgcggc
     1381 gctgctggtg gtggcgatcc acacctcgcc gctgctctcg ctgggcgaga ccgctgattt
     1441 tctgctcacc cgggtggccg cgcgggtggc ggtgccgttt tttctcatga ccagcggctt
     1501 ttttctgctg cccggcctgc ccgcgcacgg cggcttcacc aaccggcagc ggcaggcgct
     1561 gggacgtttc ttccggcgca ccgccctgct ctggggcgcg gcgctgctgc tctatctgcc
     1621 cctcaacctc tatggcggca agctctcggc taacgccccg ggcgagcctc tgcgctattt
     1681 tctgctggag ggcggctttt accatctctg gtatctgccc gcggcgctgc tgggcatggc
     1741 gctctgcctg gccctgctgc ggctggggcg caccgccgcg ctgggcgccg ccggcgcgct
     1801 ctatctggtg ggggtctttg gggacagctg gtggggtgtc gccgccaagc tgcccccgct
     1861 gcggtcgttc tacgacggcc tgctgggctt ttttggcgag acccgcaacg gcct
//
LOCUS       DBMDYA010000579         1910 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12166_length_1910_cov_1.561636, whole genome shotgun sequence.
ACCESSION   DBMDYA010000579 DBMDYA010000000
VERSION     DBMDYA010000579.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12166_length_1910_cov_1.561636"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="ACZPFK_12090"
     CDS             <1..532
                     /locus_tag="ACZPFK_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1407089.1"
                     /translation="EVDTFERMELIKDLRTGAIDVIVGINLLREGLDLPEVTLVAILD
                     ADKEGFLRSETSLIQTIGRAARNAEGKVIMYADTVTPSMERALTETDRRRRIQMAYNE
                     AHGIVPTTIKKEIRDGIGISTTDDDENRGHRMSKMEKEKLIERLTREMKEAAKILEFE
                     QAAFLRDRIEKLRKGK"
     gene            535..>1910
                     /locus_tag="ACZPFK_12095"
     CDS             535..>1910
                     /locus_tag="ACZPFK_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR1407090.1"
                     /translation="MSRDKIIIKGAREHNLKDIDVELPREKLIVMTGLSGSGKSSLAF
                     DTIYADGQRRYVESLSSYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARIGVPHCPICGREIHQQTVDQMVDAILKLPQGTRFQVLAPVVRGR
                     KGTHVKEFDAAARSGFARVRVDGNMYELDEEIKLEKNLKHTIEIVVDRLVISEDVRSR
                     LADSLETALSLTGSIVTVDVIGGKEMTFSQNYACPEHGVSVEELAPRMFSFNNPFGAC
                     ETCTGLGTFMRVDPELILPHKNLSIKQGAIKASGWYYAAGSISEMYYKGLGQKYGFTL
                     DTPIKDMSKEAVQALLYGTRGEKLTLYRETDSGTGRYTTDFEGIVNNLERRFRETSSE
                     WTKDEIAGFMTGVECPDCHGDRLKKTSLAVTVGGINISRFTQMSVKEELAFLDKIELS
                     EREHLIGDR"
BASE COUNT          420 a          594 c          604 g          292 t
ORIGIN      
        1 cgaggtggac acctttgagc gcatggagct catcaaggac ctgcgcaccg gcgccatcga
       61 cgtcatcgtg ggcattaacc tgctgcgcga ggggctggac ctgcccgagg tgacgttggt
      121 ggccatcctg gacgcggaca aagagggctt tctgcgcagc gagaccagcc tgatccagac
      181 catcggccgg gccgcccgca acgcggaggg caaggtcatc atgtatgctg acacggtcac
      241 ccccagcatg gagcgggcgc tcaccgagac cgaccgccgc cgccgcatcc agatggccta
      301 caacgaggcc cacggcatcg tgcccaccac catcaaaaag gagatccggg acggcatcgg
      361 catctccacc accgacgacg acgagaaccg cggccaccgc atgagcaaga tggaaaagga
      421 aaagctcatc gagcggctga cccgcgagat gaaagaggcg gccaagattc tggagttcga
      481 gcaggccgct ttcctgcgcg accgcatcga gaaattgaga aagggcaaat aactttgagc
      541 agagataaga tcatcatcaa gggcgccaga gagcacaatt taaaggacat cgacgtggag
      601 ctgccgcgcg aaaagctgat cgtcatgacc ggcctgtccg gcagcggcaa gtccagcctg
      661 gcgttcgaca ccatctacgc cgacggccag cgccgctatg tggagagcct gtccagctat
      721 gcgcgccagt ttttgggcca gatggacaag ccggatgtgg actccatcga ggggctgtcg
      781 ccggccatct ccatcgacca gaagacgacc tcccgcaacc cgcgctccac cgtgggcacg
      841 gtcaccgaga tctacgacta tctgcgcctg ctctacgcgc gcatcggcgt cccgcactgt
      901 cccatctgcg gccgggagat ccaccagcag acggtggatc agatggtgga cgccatcctg
      961 aagctgccgc agggcacccg ctttcaggtg ctggcgccgg tggtgcgggg gcgcaagggc
     1021 acccatgtaa aagagttcga cgccgcagcg cgcagcggct ttgcgcgcgt gcgggtcgac
     1081 ggcaacatgt acgagctgga cgaggaaatc aagctggaaa agaacctcaa gcacaccatc
     1141 gagatcgtgg tggaccgcct ggtgatcagc gaggatgtgc gcagccgtct ggcggattcg
     1201 ctcgagacgg cgctgagcct caccggcagc atcgtcaccg tcgatgtgat cggcggcaag
     1261 gagatgacct tttcacagaa ttacgcctgc cccgagcacg gcgtctcggt ggaggagctc
     1321 gccccgcgca tgttcagctt caacaacccg ttcggcgcct gcgagacctg cacgggcctg
     1381 ggcactttca tgcgggtgga cccggagctc atcctgccgc acaagaacct ctccatcaag
     1441 cagggggcca tcaaggccag cggctggtat tatgcggccg gctcgatcag cgagatgtac
     1501 tacaagggcc tggggcaaaa gtacggcttc acgctggaca cccccatcaa ggacatgtcc
     1561 aaagaggcgg tacaggccct gctctacggc acgagggggg agaagctgac cctctaccgg
     1621 gagaccgaca gcggcaccgg ccgttacacc accgattttg agggcattgt caacaacctg
     1681 gagcggcgct tccgcgagac tagcagtgag tggacaaaag acgagatcgc cggttttatg
     1741 accggcgtcg agtgcccgga ctgccacgga gaccggctga aaaagacctc gctggccgtc
     1801 acggtgggcg gcatcaacat cagccgcttt acccagatgt cggtcaagga ggagctcgct
     1861 tttctggaca agatcgagct cagcgagcgc gagcacctga tcggcgaccg
//
LOCUS       DBMDYA010000580         1910 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12167_length_1910_cov_1.296849, whole genome shotgun sequence.
ACCESSION   DBMDYA010000580 DBMDYA010000000
VERSION     DBMDYA010000580.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12167_length_1910_cov_1.296849"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="ACZPFK_12100"
     CDS             <1..416
                     /locus_tag="ACZPFK_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407091.1"
                     /translation="STFLLFGLWNILPWLLGARPVGIGNVTHVWLTLWYKLLPCLFFL
                     GSALIVYKILLTLGCKQKKALLGLYLFTTSPIAIFSQFVFVQYDSFTVFFVLLGIWLY
                     LKKKPWGFILSLGVAATFKYYALVILVVFLLLRDS"
     gene            complement(420..716)
                     /locus_tag="ACZPFK_12105"
     CDS             complement(420..716)
                     /locus_tag="ACZPFK_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407092.1"
                     /translation="MSIGMRSVYWVEVLRLIPTKDHLDREKLDAVLRWFDPEDLSNLE
                     PLKVASDEQQNLYLLDGHCRAWALVGNRGGESAGGGGKKPSPLRWFSFERSLPT"
     assembly_gap    849..858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            949..>1910
                     /locus_tag="ACZPFK_12110"
     CDS             949..>1910
                     /locus_tag="ACZPFK_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1407093.1"
                     /translation="MHYLDNAATTVVDPELAKLCYDTMLEVFGNPSSLYKPGMIAERA
                     VDHARAQVAATLGVTAAELFFTACGSESDNIALQGAAERTKNWAKNIVVTGFEHPAVY
                     RTLELLAARGWEIRTVPPDAEGHIDETAFLDAVDKQTGVVAFMFVNNEIGTLLDPVAL
                     AKRVRAKNSRASIHVDGVQGWCKHPLRLSGGEIDSFALSGHKMHCPKGVGALYLRKNY
                     RIGSVFGGGGQERGLRPGTENVPYIVTLGAAAEKYGKTIAPRLAAAGELNALLRAELA
                     RRPGVTVNSPADASPYVLNFSIPGVRSETMLHFLESKEVYVSSGS"
BASE COUNT          365 a          516 c          564 g          455 t
ORIGIN      
        1 cgagtacttt cttacttttt ggcctttgga atattttacc ttggcttctg ggagcccgtc
       61 ctgtaggcat tggtaatgta actcatgtat ggctgacttt gtggtataaa ttgttacctt
      121 gtcttttctt tttgggcagc gctctgatag tttacaaaat attgttaacg ttgggatgca
      181 aacagaaaaa agcgttgctc ggcttatatc tttttacgac atctcccata gccatattta
      241 gtcaattcgt atttgttcaa tacgacagct ttacagtttt ttttgttttg cttgggattt
      301 ggctgtatct aaaaaagaaa ccctggggat ttattctttc attaggagtc gcggcgactt
      361 ttaagtatta tgcgctcgtt atacttgtgg tttttttgtt gttaagagat agttaaagtt
      421 taagttggga gagatctttc aaaggaaaac cagcgaagag gagatggctt ttttccacca
      481 ccaccggcgc tttctccacc cctgttgccc accagggccc aggcgcggca gtggccgtcc
      541 agcagataga ggttttgctg ctcgtcgctg gcgaccttga gcggctctaa gttactcagg
      601 tcctcggggt cgaaccagcg cagcacggcg tccagctttt cccggtccag atggtccttg
      661 gtggggatca gccgcagcac ttccacccag tagacgctcc tcatcccaat gctcattttt
      721 gctcctcctc aaagacgctc ccgctgcgcc cagcttttgc gtgtctgggc ggaaaggcga
      781 ggtggcgggt tcgtttctgc attttaactt ttttcatttt aaacattccg ggcccggcgc
      841 gcaagggcnn nnnnnnnnat ttggtcaaaa actgtgagcg ctgaggcggt tttaacgctg
      901 cctcatgccc tgaaaaaccg ccccgaacgg gcgtcggagg cgttttgtat gcactatctc
      961 gacaatgcgg ccaccacggt ggtggacccc gagttggcaa agctctgcta cgacacaatg
     1021 ctggaggtct tcggtaaccc ctcctcgctc tacaagccgg ggatgatcgc cgagcgggcg
     1081 gtggatcacg cccgcgccca ggtggcggcg acgctggggg tgacggcggc ggagctcttt
     1141 tttaccgcct gcgggtcgga gagcgacaac atcgccctgc agggggcggc ggagcgcacc
     1201 aaaaactggg ccaagaatat tgtggtcacc ggctttgagc acccggcggt ttaccgcacg
     1261 ttggagctgt tggcggcgcg gggctgggag atccgcaccg tcccgccgga cgcagaggga
     1321 cacatcgacg agacggcttt tctggacgcg gtggacaagc agaccggcgt cgtggccttt
     1381 atgtttgtca acaacgagat cggcaccctg ctggacccgg tggcgctggc aaaaagggtc
     1441 cgcgcaaaaa acagccgcgc ctccatccat gtggacggcg tccagggctg gtgcaagcat
     1501 ccgctgaggc tgtccggagg cgagatcgac agctttgccc tctccggcca caagatgcac
     1561 tgccccaagg gcgtcggcgc gctttatctg cgcaaaaatt accgtatcgg gtcggtcttc
     1621 ggcggcggcg ggcaggagcg cggtctgcgt ccgggcactg aaaacgtacc ctatatcgtc
     1681 acgctggggg cggcggcgga aaaatacggc aagaccatcg cccctcggct ggcggcggca
     1741 ggggagctga acgcgctgct gcgggcggag ctggcccggc ggccgggcgt cacagtgaac
     1801 tccccggcgg acgcttcgcc gtatgtgctc aacttttcca tccccggcgt gcgcagcgag
     1861 acgatgctgc actttttaga gagcaaagag gtttacgtct ccagcggttc
//
LOCUS       DBMDYA010000581         1909 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12173_length_1909_cov_1.324668, whole genome shotgun sequence.
ACCESSION   DBMDYA010000581 DBMDYA010000000
VERSION     DBMDYA010000581.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12173_length_1909_cov_1.324668"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="ACZPFK_12115"
     CDS             complement(<1..92)
                     /locus_tag="ACZPFK_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uS14 family ribosomal protein"
                     /protein_id="HIR1407094.1"
                     /translation="MAKTSMKVRQSREPKFSTRGYNRCKICGRP"
     gene            complement(106..645)
                     /gene="rplE"
                     /locus_tag="ACZPFK_12120"
     CDS             complement(106..645)
                     /gene="rplE"
                     /locus_tag="ACZPFK_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1407095.1"
                     /translation="MARLKDKYASEIAPALMSKFGYKSVMQIPKLDKVVINCGCGEAR
                     DNPKIMDAVLADLAAITGQHPVVCKAKKSVANFKLREGMPIGAKVTLRGDKMYEFVDR
                     LFSVALPRVRDFRGINGDSFDGRGNYSLGIKEQLIFPEIDYDKIDAIRGMDIVFVTTA
                     KTDEEAKELIRALGAPFAN"
     gene            complement(662..976)
                     /gene="rplX"
                     /locus_tag="ACZPFK_12125"
     CDS             complement(662..976)
                     /gene="rplX"
                     /locus_tag="ACZPFK_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1407096.1"
                     /translation="MSKVHVKTGDTVMIISGKDAGKSGKVLQVSPKEGKVIVEGLNMV
                     TKHVKPRREGEQGGIVKAEGALYAAKVMPVCPKCGKATRVGHIVNNGKKVRVCKKCGA
                     EF"
     gene            complement(989..1351)
                     /gene="rplN"
                     /locus_tag="ACZPFK_12130"
     CDS             complement(989..1351)
                     /gene="rplN"
                     /locus_tag="ACZPFK_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1407097.1"
                     /translation="MQTYLKVADNTGAKELMCIRVLGGSRKRYANIGDVIVASVKKTT
                     PGGGMVKKGEVVKAVIVRSKKGLRRDDGSYIRFDENAAVIIKEDKNPRGTRIFGPVAR
                     ELREAGYLKILSLAPESL"
     gene            complement(1389..1646)
                     /gene="rpsQ"
                     /locus_tag="ACZPFK_12135"
     CDS             complement(1389..1646)
                     /gene="rpsQ"
                     /locus_tag="ACZPFK_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1407098.1"
                     /translation="MEERNLRKTRVGTVVSDKMDKTIVVAIEDNVSHPLYKKIIKRTV
                     KFKAHDEKNEAKAGDRVKIMETRPLSKDKRWRLVEIVEKAK"
     gene            complement(1666..1863)
                     /gene="rpmC"
                     /locus_tag="ACZPFK_12140"
     CDS             complement(1666..1863)
                     /gene="rpmC"
                     /locus_tag="ACZPFK_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1407099.1"
                     /translation="MKANELTKMSAADLDKKLAELKEELFNLRFQHTINQLDNPLRIK
                     AVKRDIARVKTAQRMLADKAE"
BASE COUNT          309 a          573 c          522 g          505 t
ORIGIN      
        1 tgagggcggc cgcagatctt gcagcggttg tagccacggg tcgagaactt cggctcgcgg
       61 ctctgccgca ctttcatgga tgttttagcc attgaaaccc ctcccttagt ttgcgaacgg
      121 agcgcccaga gcacggatga gctctttggc ctcctcgtcg gtcttcgcgg tggtgacgaa
      181 aacaatgtcc ataccgcgga tcgcgtcgat cttgtcgtag tcgatctccg ggaagatcag
      241 ctgctccttg atgcccagcg aatagttgcc gcggccgtcg aaagaatcgc cgttgatgcc
      301 gcggaagtcg cgcacgcggg gaagtgccac gctgaacagg cggtcgacga actcgtacat
      361 cttgtcgccc cgcagggtga ccttggcgcc gatgggcatg ccctcgcgca gcttgaagtt
      421 cgcaaccgac tttttcgcct tgcagacgac cgggtgctgg ccggtgatcg cggccaggtc
      481 cgcgagaacg gcgtccatga tcttcgggtt gtcacgggcc tcgccgcagc cgcagttgat
      541 gacgaccttg tcgagcttgg ggatctgcat cacgctcttg tagccgaatt tgctcatcag
      601 ggcgggagcg atctcgctgg cgtatttgtc ttttaatctt gccatctgtc aacttcctcc
      661 cttaaaattc cgcgccgcat tttttgcaga cgcgcacttt tttgccgttg ttcacgatgt
      721 ggcccacacg ggtggccttg ccgcacttgg ggcagaccgg catgaccttg gcggcgtaca
      781 gcgcgccctc ggcctttacg atgccgccct gctcgccctc acggcggggc ttgacgtgct
      841 tggtgaccat gttcaggccc tcaacgatga ccttgccctc cttggggctg acctgcaata
      901 ccttgccgga tttcccagcg tccttgccac tgatgatcat gacggtatcg ccggtcttaa
      961 catgaacttt actcatttta aaacctcctt acagcgattc cggggcaagg ctcaggatct
     1021 tcagatagcc ggcctcgcgc agttcgcggg ccaccggtcc aaagatacgg gtgcctctcg
     1081 ggtttttgtc ctctttgatg atgacggccg cattctcgtc gaagcggatg taggagccgt
     1141 cgtcgcggcg caggcccttt ttgctgcgga cgataacggc ctttacgacc tcgccctttt
     1201 tgaccatgcc gccgcccggc gtagtcttct tgacagaagc tacgatcacg tcgccaatat
     1261 tagcgtatct ctttcgggaa ccgcccagca cacgaataca catgagttcc ttcgcaccgg
     1321 tgttgtcggc cactttgaga taggtttgca tctgtaccac agttgcgtcc tcctttcaaa
     1381 gctttcagtt atttcgcctt ttctacgatc tcgaccagtc tccagcgctt gtccttggac
     1441 agcgggcggg tctccatgat cttcacgcgg tcgccggctt tggcctcgtt cttctcgtcg
     1501 tgggccttga acttgacggt gcgtttgatg atctttttat agagagggtg gctcacgttg
     1561 tcctcgatcg ccacaacgat ggttttgtcc atcttgtcgg acacgacggt gcccacgcgg
     1621 gtcttgcgta aattgcgctc ttccattctt taactcctcc ctctcttatt cagccttgtc
     1681 tgcgagcatg cgctgcgcgg ttttcacgcg ggcgatgtca cgcttgacgg ccttgatcct
     1741 aagcgggttg tcgagttggt tgatggtgtg ctgaaagcgc aggttgaaca gctcttcctt
     1801 cagctcggcc agctttttgt ccaggtcggc cgcactcatc ttagtgagtt cgttcgcttt
     1861 catgccttgg caacctcctc ttcttctctc ttcacaaact tgcacttga
//
LOCUS       DBMDYA010000582         1904 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12223_length_1904_cov_2.230172, whole genome shotgun sequence.
ACCESSION   DBMDYA010000582 DBMDYA010000000
VERSION     DBMDYA010000582.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12223_length_1904_cov_2.230172"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="ACZPFK_12145"
     CDS             complement(<1..114)
                     /locus_tag="ACZPFK_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1407100.1"
                     /translation="MQYSAKVMDHFSNPRNVGEIADADGVGEVGNPTCGDIM"
     gene            complement(180..1388)
                     /gene="nifS"
                     /locus_tag="ACZPFK_12150"
     CDS             complement(180..1388)
                     /gene="nifS"
                     /locus_tag="ACZPFK_12150"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR1407101.1"
                     /translation="MEQAGKVIYLDNAATTPVSAPVLDAMLPYFSENYGNPSSIYGIA
                     RRSKEAIDTARAQVAAALNCFPEEIFFTGCGSESDNWVVKGVAHKLGAQGKRHLVTTA
                     IEHHAILHSMAALEKEGFEVTYLPVDADGLVSADQVRAALRPDTALVSVMYANNEIGT
                     IEPVAEIGAVCREAGVLFHTDAVQATGAVKIDVRAQNIDALSLSAHKFNGPKGVGALY
                     LKKGLRLPNLIDGGGQESGRRAGTENVAGIVGLGRALELANAQLTEKSAHLAQLRDYV
                     IRTVEQSIPNVKLNGHPTRRLPNNVNLSFEGIEGEGMLLLLDMKGICASSGSACTSGS
                     LDPSHVLLAIGLPHEIAHGSLRLTFGPQNTMEDAEAVCNALREIIPRLRAMSPVWDKI
                     EKGEAKPLLG"
     gene            complement(1667..>1904)
                     /locus_tag="ACZPFK_12155"
     CDS             complement(1667..>1904)
                     /locus_tag="ACZPFK_12155"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR1407102.1"
                     /translation="LMFRYLPIEEQLSTPDLGRYRSFGIAAFQVLEGRWEKKAFASDV
                     SADEVFLTRLAQRCTQAQLNPIHLRDVVEDTIGR"
BASE COUNT          350 a          581 c          556 g          417 t
ORIGIN      
        1 catgatgtcg ccgcaggtgg ggttgcccac ctcaccgacc ccgtcggcgt cggcgatctc
       61 ccccacattg cgcgggttgg aaaaatgatc catcactttt gcactgtatt gcatccgtaa
      121 tctctcctta ctatatcaaa agtctgccaa aatgtacagg ggaccggcag gccgcgcgtt
      181 cagcccagca gcggctttgc ctctcctttc tctatcttgt cccacaccgg gctcatggcc
      241 cgcaggcggg ggatgatctc ccgcagcgca ttgcagaccg cctcggcgtc ctccatggtg
      301 ttctggggac cgaaagtcag ccgcaggctg ccgtgggcga tctcgtgggg caggccgatg
      361 gccagcagca catggctggg gtccaggctg cccgaggtgc aggcggagcc ggaggaggcg
      421 cagatgcctt tcatgtcgag caaaagcagc atgccctcgc cctcgatccc ctcaaaagag
      481 aggttgacat tgttgggcag ccgccgggta gggtggccgt tcagcttgac attggggatg
      541 ctctgctcca cggtgcggat cacatagtcg cgcagctggg ccagatgggc ggatttttcc
      601 gtcagctgcg cgtttgccag ctccagcgcc cggcccagcc ccacgatacc ggccacattt
      661 tcggtgcccg cgcgccggcc gctctcctgc ccgcctccgt cgatgaggtt gggcagccgc
      721 agcccttttt tcagatagag ggcgccgacc cccttggggc cgttgaactt gtgcgcggac
      781 aggctgagcg catcgatgtt ttgcgcccgc acgtcgatct tcaccgcgcc tgtggcctgc
      841 accgcatcgg tgtgaaagag gaccccggcc tcgcggcaga cggcgccgat ctccgccacc
      901 ggctcgatgg tgccgatctc attgttggcg tacatgacgg ataccagagc ggtgtccggg
      961 cgaagcgcag cccgcacctg gtcggcagaa accaggccgt cggcgtccac cggcagataa
     1021 gtgacctcga acccctcctt ttccagcgca gccatgctgt gcaggatggc gtgatgttcg
     1081 atggcggtgg tgacgaggtg ccgtttgccc tgggctccca gcttgtgggc gacgcccttg
     1141 accacccagt tgtcgctctc ggagccgcag ccggtgaaaa aaatttcctc cggaaagcag
     1201 ttgagggcgg cggcgacctg ggcgcgggca gtgtcaatgg cctctttgct gcgacgcgca
     1261 ataccgtaaa tgctggaggg gttgccatag ttttcgctga aatagggcag catggcgtcc
     1321 agcaccgggg ccgacaccgg cgtggtggcc gcgttatcca gataaataac cttaccagct
     1381 tgttccaact gaatcacctg tgctttctat ttgatttcct tgtttaatat atactatttt
     1441 actaggaatt gcaacaggga atccatatta ttttagtggg atatcttttt tttgcttacc
     1501 gccccttgaa aaagcgctct tttgcacggt gtttcgtgat ctctgcgccg cttttttgag
     1561 gttctaccct gaactatttt tgcagtttcg cttttggcgg ctgtctccgc cccgtccaaa
     1621 aataaaaaga gggggcccgg ccctctccaa aggcccggcc cctgctttat cgcccgatgg
     1681 tgtcctctac cacatctctc agatggatag gatttaactg ggcctgggta cagcgctggg
     1741 caagacgggt caaaaaaacc tcgtccgcgg acacatcgga ggcgaatgcc ttcttttccc
     1801 atcggccctc caaaacctga aacgccgcga tcccgaaaga gcggtagcgc cccaagtcag
     1861 gggtggacaa ctgctcttca atgggcagat accgaaacat caag
//
LOCUS       DBMDYA010000583         1900 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12260_length_1900_cov_1.560867, whole genome shotgun sequence.
ACCESSION   DBMDYA010000583 DBMDYA010000000
VERSION     DBMDYA010000583.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12260_length_1900_cov_1.560867"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="ACZPFK_12160"
     CDS             <1..915
                     /locus_tag="ACZPFK_12160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1407103.1"
                     /translation="EMKQSDFSGILTQGGTILGTKRTPFKMMQVIEDDQVDKVAEMKK
                     TYKKAKLDCLLCLGGNGTHKTANLLSQEGLNIIGLPKTIDNDIWGTDVTFGFHTAVDI
                     ATEVIDRIHTTASSHRRVMVIELMGNKAGWLTLYSGIAGGADIILIPELPYDMDKVIK
                     AIEKRNANGRTFSILAVAEGAYDVEEAKMKKKERAAARAAAGVKTATNRIAADIERIT
                     GFETRVCVPGHMQRGGTPSAYDRVLATQFGAYAAKLVAEDKYGVAVAMVNSVVTDNPL
                     PEIAGKSKLVPADCPMLDLARSMGVSLG"
     gene            complement(902..1219)
                     /locus_tag="ACZPFK_12165"
     CDS             complement(902..1219)
                     /locus_tag="ACZPFK_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407104.1"
                     /translation="MPELFSFSSPLVSNLYPFSPGCACRRAPSAQGPAAAPLGSLKAV
                     ANRYHFNSKKFYLSIHNRAKTCKNFHFLLKKRFFMQKQPVSETGGRLLRLPRFHRAGF
                     NPG"
     gene            1201..>1900
                     /locus_tag="ACZPFK_12170"
     CDS             1201..>1900
                     /locus_tag="ACZPFK_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1407105.1"
                     /translation="MKKVLALLLAAAMAFSMVACGGNGGSSAAAGGNGGGGAAAGGDG
                     PRTSMVYGMSAEPSTLDPMNIASMNTFTVTYALYDTLTAPDGNGSYVPYLCDDVKVSE
                     DGLTYTILLNKEVKFQDGTTLTTEDIAFSIQRTIAAGWAADMARYIESVEAVDENTVV
                     MTLNAPFNGMMGSLASPFFSIMSKAYCEEQGDEGLKRAPMGTGAYKLAEWVSGDHITL
                     EANEDYFAGAPAIKT"
BASE COUNT          410 a          592 c          593 g          305 t
ORIGIN      
        1 gagatgaagc agagtgattt ttccggcatc ctgacccaag gcggcaccat cctgggcacc
       61 aagcgcaccc cgttcaagat gatgcaggtc atcgaggacg accaggtgga caaggtggcc
      121 gagatgaaaa agacctacaa aaaggccaag ctggactgcc tgctctgcct gggcggcaac
      181 ggcacccaca agacggcgaa cctgctgtcg caggaggggc tgaacatcat cggcctgccc
      241 aagaccatcg acaacgatat ctggggcacc gacgtgacgt tcggctttca caccgcggtg
      301 gacatcgcca ccgaggtcat cgaccgcatc cacaccaccg cctccagcca ccgccgagtc
      361 atggtcatcg agctgatggg caacaaggcg gggtggctca ccctttacag cggcattgct
      421 ggcggcgcgg atatcatcct catccccgag ctgccctatg acatggacaa ggtcatcaag
      481 gccatcgaaa agcgcaatgc caacggccgc acgttctcga tcctcgccgt ggccgagggc
      541 gcctacgacg tcgaagaggc caagatgaaa aagaaagagc gggccgccgc ccgtgccgcc
      601 gctggcgtca agacggcgac gaaccgcatt gcagcggaca tcgagcgcat caccggcttt
      661 gagacccggg tctgcgtgcc cggccatatg cagcgcggcg gcaccccctc cgcttacgac
      721 cgggtgttgg ccacccagtt cggggcctat gccgcaaagc tggtggccga ggacaagtac
      781 ggcgtggcgg tggcgatggt caacagcgtc gtgacggaca accccctgcc cgagatcgcg
      841 ggcaagagca agctggtgcc cgccgactgc cccatgctgg atctggcgcg cagcatgggc
      901 gtcagcctgg gttgaacccg gcgcggtgaa agcgcggcag gcgcaacagc cggcctccgg
      961 tttcggagac cggctgtttt tgcataaaaa agcgcttttt taaaagaaaa tgaaaatttt
     1021 tgcaggtttt cgccctgtta tgtattgaca aataaaattt tttgctgtta aaatgatatc
     1081 tgtttgcaac tgctttcaaa gagcccagcg gcgcggccgc cggaccctgg gcagaggggg
     1141 cgcgccggca ggcgcacccc ggagaaaagg gatacagatt tgagacaagg ggagaagaaa
     1201 atgaaaaaag ttctggcatt gctgctggct gccgcgatgg cgttttcgat ggtagcctgc
     1261 gggggcaacg gcggcagcag cgcggctgcg ggcggcaacg gtggcggcgg cgctgccgcg
     1321 ggcggcgacg gcccccgcac ctcgatggtc tacggcatga gcgccgagcc cagcaccctg
     1381 gacccgatga acatcgccag catgaacacc tttaccgtca cctacgcgct gtacgacacc
     1441 ctcaccgcac cggacggcaa cggcagctat gtgccgtatc tgtgcgacga cgtgaaggtt
     1501 tcggaggacg gcctcaccta caccatcctc ctcaacaaag aggtcaagtt ccaggacggc
     1561 accaccctca ccaccgagga catcgcgttt tccatccagc gcaccatcgc ggccggctgg
     1621 gctgcggata tggcgcgcta catcgagagc gttgaggccg tggacgagaa caccgtcgtc
     1681 atgaccctga acgcgccgtt caacggcatg atgggctcgc tggcctctcc gttcttctcc
     1741 atcatgagca aggcctattg cgaggagcag ggcgacgagg ggctcaagcg cgcgcccatg
     1801 ggcaccggcg cttacaagct ggccgagtgg gtctcgggcg accacatcac gctggaggcg
     1861 aacgaggatt acttcgccgg cgcgcccgcc atcaagacca
//
LOCUS       DBMDYA010000584         1898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12279_length_1898_cov_1.661658, whole genome shotgun sequence.
ACCESSION   DBMDYA010000584 DBMDYA010000000
VERSION     DBMDYA010000584.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12279_length_1898_cov_1.661658"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            73..774
                     /locus_tag="ACZPFK_12175"
     CDS             73..774
                     /locus_tag="ACZPFK_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR1407106.1"
                     /translation="MPLSYFLFYNCMYGLAASFTVPLFMLRREKNILSSVGIKKLGIR
                     QYGVLTAFVLLSIGGQLLPQMIAGETIPWHFLPMGIVPLSMTTFFEEFLFRGFVQTRA
                     EKSFGRPAAILISGLMFSLYHLGYPGFRTFEELLLLFFVGLGFAFAYQLSGDNLIVSF
                     SVNLPNAFVTYVLRHGQFPAMDFSATIAGGVTLAVVALILCWVWRKRPAIGRREWMPP
                     AATKIESGWESKAFK"
     gene            813..>1898
                     /gene="nagA"
                     /locus_tag="ACZPFK_12180"
     CDS             813..>1898
                     /gene="nagA"
                     /locus_tag="ACZPFK_12180"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1407107.1"
                     /translation="MQIKNGTLYTQGFFFEPHDLITSGERIARIAPTGIDTPETEVVD
                     ASGCYVVPGLVDIHIHGAAGSDFCDGTPQAVDAIAAYLGAQGVTSFLGTSMAFGEERL
                     TAIFESARPLVGADRGGAVLRGINMEGPFFSRAKKGAQAEEYLRDPDTDLFDRLFEAS
                     GRNVRLVDVAPELPGALPFIEHAARRCWVSLAHTAADYEQARAGFRAGADHVTHLFNA
                     MPPFGHREPGVVGAAADLAGYVELISDGIHLHPATVRAAFGLFGAERVCLISDAMSAC
                     GMPDGSYELGGQAVRVEQGRATLADGTIAGSATCLTDCLRRAVSFGVPLEDALRAATA
                     NPAKSVGLYEELGSLSEGKRADLLVLDK"
BASE COUNT          307 a          549 c          626 g          416 t
ORIGIN      
        1 cgcacggctt gctgacagcg agtcttttta gtcttgctta aggtcccggc agggccggga
       61 tgggcagccg tcatgcctct gtcctatttt cttttttaca attgtatgta tggcttggcg
      121 gccagcttta cagtccctct ttttatgctg cgcagagaga aaaatatctt gtcttccgtg
      181 gggattaaaa aattggggat ccggcaatac ggcgttttga ccgcatttgt cctgctttcg
      241 atcggcgggc agcttcttcc gcagatgatt gccggtgaaa cgatcccttg gcatttttta
      301 cctatgggaa ttgtgccgct gagcatgacc acgttctttg aggagttttt atttcgcgga
      361 tttgtgcaga ccagggcgga aaagagcttt gggcggcccg ctgcgattct gatctcaggg
      421 ctcatgtttt cgctttatca tctgggatat cccgggtttc gcacctttga agagcttctt
      481 cttttatttt ttgtgggcct tggatttgcc tttgcctatc aactttccgg cgacaatctg
      541 atcgtttctt tctctgtaaa tctgcccaat gcttttgtta cctatgtgct gaggcacggg
      601 caatttcccg ctatggattt cagcgccacg atagcgggcg gcgttaccct ggctgtggtc
      661 gcgctgatcc tttgctgggt gtggcgcaag aggccggcaa ttggccgcag ggaatggatg
      721 cccccggcgg cgacaaaaat agaaagcgga tgggaatcaa aggccttcaa ataaatttgt
      781 gccgctgtaa agcggcggaa tggatgaatt ttatgcagat caagaacgga actctttaca
      841 cccagggctt tttctttgaa ccccacgacc tgatcaccag cggggagaga atcgcccgca
      901 tcgcgccgac cggcatcgac acccccgaga cggaggtcgt ggacgcttcg ggctgctacg
      961 tggtgcccgg gctggtggac attcatatcc acggtgcggc gggcagcgat ttctgcgacg
     1021 gcacgccgca ggcggtggac gccatcgccg cttatttggg cgcgcagggg gtgaccagct
     1081 ttctgggcac cagcatggcc tttggagaag agcggctgac cgccatcttc gagagcgccc
     1141 ggccgctggt tggggcggac cggggcggcg cggtgctgcg cggcatcaac atggaggggc
     1201 cctttttctc ccgggcaaaa aagggcgccc aggcggaaga gtatctccgg gaccccgaca
     1261 cggacctgtt cgaccggctg ttcgaggcct cgggccgcaa tgtgcggctg gtggacgtgg
     1321 cgccggagct gccgggcgcg ctgccgttca tcgagcacgc cgcgcggcgc tgctgggttt
     1381 cgctggccca cacggcggct gattacgagc aggcgcgggc gggctttcgc gccggggcgg
     1441 accacgtcac ccatctgttc aacgccatgc cgccctttgg ccaccgggag ccgggcgtcg
     1501 tgggcgccgc cgccgacctg gccggctatg tggagctgat ctcggacggc atccacctgc
     1561 acccggccac ggtgcgggcc gctttcgggc ttttcggcgc ggagcgggtc tgcctgatca
     1621 gcgacgccat gagcgcctgc ggtatgccgg acggcagcta cgagctgggc ggccaggccg
     1681 tccgggtgga gcaggggcgc gccacgctgg ccgacggcac catcgcgggc agcgccacct
     1741 gcctgacgga ttgcctgcgg cgggcggtct ctttcggcgt gccgctggag gacgccctgc
     1801 gggccgccac cgccaacccg gcaaagtcgg tgggcctgta cgaggagctc ggcagcctgt
     1861 cggagggcaa gcgcgccgac ctgctggtgc tggacaag
//
LOCUS       DBMDYA010000585         1897 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12296_length_1897_cov_1.239421, whole genome shotgun sequence.
ACCESSION   DBMDYA010000585 DBMDYA010000000
VERSION     DBMDYA010000585.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12296_length_1897_cov_1.239421"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(135..389)
                     /gene="rpsR"
                     /locus_tag="ACZPFK_12185"
     CDS             complement(135..389)
                     /gene="rpsR"
                     /locus_tag="ACZPFK_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1407108.1"
                     /translation="MERTERPERSGRPQRRGRKKVCSFCVDRVDHIDYKDVVKLRKYI
                     SERSKIIPRRVTGTCAEHQRELTVAIKRARHLALLPFVSD"
     gene            complement(417..878)
                     /locus_tag="ACZPFK_12190"
     CDS             complement(417..878)
                     /locus_tag="ACZPFK_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1407109.1"
                     /translation="MLNVVALLGRLTADPELRKTPNGVSVTTFTVAVNRSYVKQGGER
                     QTDFIDVVAWRSTAEFITKYFKKGQMIAVQGSIQTRSYEDKQGNKRKAVEISADNVYF
                     ADSKGSSQGGQHNNSYGAPDYSSAPAPAAPSFSQGSADDFAVVDDNEDLPF"
     gene            complement(891..1175)
                     /gene="rpsF"
                     /locus_tag="ACZPFK_12195"
     CDS             complement(891..1175)
                     /gene="rpsF"
                     /locus_tag="ACZPFK_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1407110.1"
                     /translation="MKKYETVMVFSTAITEEETQALVEKFKNLIAKNGTVESVDDWGK
                     RRLAYPINDENDGYYVLVNFSCEPDFPAELDRVYKITDGVLRSLIVAKDE"
BASE COUNT          408 a          515 c          581 g          393 t
ORIGIN      
        1 ttcgccggtc tctctttccc ggataagaag ctttgaacac aaaaaaggct ttcgctttgc
       61 gaaagccttt tttgtgttca aaacagcaga gaaaaagccc gccgtgcttt gcacggcggg
      121 cttgaaaagg ctttttagtc gctcacaaag gggagcaggg ccagatgacg ggcccgcttg
      181 atggcaacgg tcagctcgcg ctggtgctcg gcacaagtgc cggtcacgcg gcgggggatg
      241 atcttggagc gctcagaaat atatttgcgc agcttgacga catccttgta gtcgatgtgg
      301 tcgacgcgat cgacgcagaa gctgcaaacc tttttgcggc cccttctctg gggacggccg
      361 cttctctcag gcctttcggt tctttccaaa gttcttacct ccttataaaa gtgggatcaa
      421 aacggcagat cctcgttgtc atccaccacg gcgaagtcat cggccgaacc ctgcgagaac
      481 gagggggcgg ccggagccgg ggcggaggag taatcgggcg caccgtagga gttgttgtgc
      541 tgtccgccct gagaagaacc cttggagtcc gcaaaataga cgttatcagc cgaaatctcg
      601 acagccttgc gcttgttgcc ctgcttgtcc tcataggaac gggtctggat gctgccctgc
      661 acggcgatca tctggccctt cttgaagtat ttggtgataa actcagcagt gctgcgccag
      721 gcgaccacgt cgatgaagtc ggtctgacgc tccccgccct gcttcacata gctgcggttg
      781 accgcaaccg tgaaagtggt gacggaaacg ccgttggggg tcttgcggag ctcggggtcc
      841 gccgtcaggc ggccgagcag tgccacaacg ttcagcataa aacttcctcc ttactcgtcc
      901 ttggcaacga tcagggaccg cagaacgccg tcggtgatct tgtacacgcg gtcgagctca
      961 gccgggaaat cgggctcgca gctgaagttg accagcacat agtagccgtc gttctcgtcg
     1021 ttgatggggt aggccagacg gcgcttgccc caatcgtcaa cagattcgac agtaccattt
     1081 ttcgcgatga ggttcttgaa cttttcgacc agagcctgcg tctcctcctc ggtaatcgcg
     1141 gtggagaaca ccatcacagt ttcgtacttc ttcattctgt acacctcctt ttggacatat
     1201 ggccccttgc ggggcaagga tcgctgcgca agcggataag ccgcctttgc acagcacttt
     1261 attatacccc ggatggggaa acaaagtcaa gagaaaaacg aaaagtcctt ttgcagagaa
     1321 ccgcccgtgc agcgggctgc ggcagcccgc tggggttgcc tttgagaaaa acgggccgat
     1381 atggtatgat gcataaaaag aggccggtcc tcttttgggc aaagcggttg aaacaaactt
     1441 cgcatgaaag aggggggcaa ggcgtgaatc ctgtattgtg gatcgcggtg gcggcggcgc
     1501 tgctctgcgc ggcaacggcc gtttactttg ggaccagaaa gaaaaagtga agccgtcctt
     1561 tcccggtcgg cccgcttgga cgcggctcag gacgccctgc ggagagaaat ctcccatcgc
     1621 ataaagggga tggggggcaa ataaaagagc gcggcacgaa aacgcagtga cgacggggga
     1681 gatatggctt tgcccggtca gaactgcgct gaaaggccgc gctctttttg gggctgccca
     1741 tgccttttgg atgttgcggc aaaacaggga cgccgcgccc ggtgcggtct cggccgtggc
     1801 gccaagaaag aaagcgggcg gaaaggccgt caaaagaggc ggcgccccga cgtcgccgca
     1861 ggggcgggcg tcacagggtg cgggccaact ctttcaa
//
LOCUS       DBMDYA010000586         1896 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12303_length_1896_cov_1.644568, whole genome shotgun sequence.
ACCESSION   DBMDYA010000586 DBMDYA010000000
VERSION     DBMDYA010000586.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_1896_cov_1.644568"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="ACZPFK_12200"
     CDS             <1..711
                     /locus_tag="ACZPFK_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014325.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1407111.1"
                     /translation="FKSLGIYRDSVKSVLIVGGGKIAFYLAAQLLHLGMRVKIVEQDE
                     ATCELLSDALPKATILHGDGTDQELLLEEGLGSIDACVSLTGIDEENIIVSMFAAMKQ
                     VKKVITKVNRANLFSIMRSVGIESVVSPRAVTGELILRYVRAMRNSKGNSVQTLYKLL
                     DGRMEALEFIVGESAPFTGKTLQALQFKKDILIAGIIRGGKLIHPQGSDVMLPGDLVI
                     VVTTTPGLRDLKDTLAEA"
     gene            719..>1896
                     /locus_tag="ACZPFK_12205"
     CDS             719..>1896
                     /locus_tag="ACZPFK_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1407112.1"
                     /translation="MNYPMVFYLIGNLLKVEAGLMVLPLLVAVFYGEDTVLSFLITIL
                     LLLIIGLLLSFRKPKDRAMYAREGFLIVGISWVLLSVFGALPFTLSGEIPRFVDAFFE
                     TVSGFTTTGASILTDVESLSRGILFWRSFTHWVGGMGVLVFVLAILPQSDAQTLYVMK
                     AEVPGPTCGKLVAKVRHTALILYGIYLVMTLLLIVLLLLGGMPLFDSVLHSFGTAGTG
                     GFGIKAASIGHYNSAYIDGVIGVFMILFGVNFNLYYFLLLGDFASVFKSEELRWYLGI
                     IAASVGLITLNILPLYGNNPLHSFRYAFFQVSSIITTTGYTTADYSAWPVFSQTILVL
                     IMFVGAMAGSTGGGIKVSRVAILFKNAVCGLRSMLSPRSVRTLKFEGKPLEKQTMAGI
                     "
BASE COUNT          334 a          600 c          550 g          412 t
ORIGIN      
        1 ttcaagagcc tcggcatcta ccgcgacagt gtgaaatccg tgctgatcgt gggcggcggc
       61 aagatcgcct tttatctggc cgcgcagctt ttgcatctgg gcatgcgggt caagatcgtg
      121 gagcaggacg aggcgacctg cgagctgctc agcgatgcgc tgcccaaggc gaccatcctg
      181 cacggcgacg gcaccgacca ggagcttttg ctggaggagg ggctgggctc gatcgacgcc
      241 tgcgtctcgc tgacaggcat cgacgaggag aatatcatcg tctcaatgtt cgccgccatg
      301 aagcaggtaa aaaaagtgat caccaaggtc aaccgtgcca acctgttctc catcatgcgc
      361 agcgtcggca tcgagagcgt cgtatccccc cgcgccgtca ccggcgagct gatcctgcgg
      421 tatgtgcgtg ccatgcgcaa ctcgaaaggc aacagcgtac agacgctgta caagctgctg
      481 gacggccgca tggaggcgct ggagtttatc gtcggcgaga gcgccccttt cacgggaaaa
      541 acgctgcagg cgctgcagtt caaaaaagac atcctcatcg ccggcatcat ccgcggcggc
      601 aaactgatcc atccgcaggg cagcgacgtc atgctgccgg gggatctggt catcgtcgtc
      661 acgaccaccc cgggcctgcg ggacctcaag gacaccttgg cggaggcttg actggcatat
      721 gaattatccc atggtctttt atctgatcgg caacctttta aaagtggagg cgggcctgat
      781 ggtgctgccg ctgctggtcg ccgtctttta cggcgaggac acggtgctgt ctttcctcat
      841 caccatcctg ctgctgctca tcatcgggct gctgctctct ttccgcaagc cgaaagaccg
      901 cgccatgtac gcgcgcgagg gctttctcat cgtggggatc tcctgggtgc tgctgtcggt
      961 attcggcgcc ctgcccttta cgctgtcggg cgagatcccc cgctttgtgg atgcgttttt
     1021 cgagaccgtg tccggcttca ccaccaccgg cgccagcatc ctcaccgatg tggagtcgct
     1081 gagccgtggc atcctgttct ggcgcagctt cacccactgg gtcggcggca tgggcgtgct
     1141 ggtctttgtg ctggccatcc tgccccaatc ggacgcgcag accctgtatg tgatgaaagc
     1201 tgaggtgccc ggccccacct gcggcaagct ggtggccaag gtgcgccaca ccgctttgat
     1261 cctttacggt atttatctgg tcatgaccct gctgctgatc gtgcttctgc tgctgggcgg
     1321 catgccgctg ttcgacagcg tcctgcactc gttcggcact gcgggcacgg gcggcttcgg
     1381 catcaaggcc gccagcatcg gccattataa ctccgcttat atcgacggcg tcatcggggt
     1441 gttcatgatc ctgttcggcg tcaacttcaa cctgtactat tttttgctgc taggcgattt
     1501 tgcaagcgtg ttcaaaagcg aggagctgcg ctggtatctg ggcatcatcg ccgcctcggt
     1561 gggcctgatc accctcaaca ttctgccgct ctacggcaat aatcccctgc acagcttccg
     1621 ctatgcgttt ttccaggtct ccagcatcat caccaccacc ggctacacca cggccgacta
     1681 cagcgcctgg cccgtcttct cccagacgat cctggtattg atcatgttcg tcggcgccat
     1741 ggcgggctcc accggcggcg gcatcaaggt ctcacgcgtg gccatccttt tcaaaaacgc
     1801 cgtgtgcggc ctgcgctcca tgctgtcccc ccgcagcgtg cgcaccctca aatttgaggg
     1861 caagcccctg gaaaaacaga ccatggccgg catcaa
//
LOCUS       DBMDYA010000587         1888 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12366_length_1888_cov_1.832680, whole genome shotgun sequence.
ACCESSION   DBMDYA010000587 DBMDYA010000000
VERSION     DBMDYA010000587.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_1888_cov_1.832680"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="ACZPFK_12210"
     CDS             <1..1408
                     /locus_tag="ACZPFK_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1407113.1"
                     /translation="ARFYFEQLNTDEGRAARGYLRGRALSDGTIKRFGLGYSPEGYHA
                     TSDYLHKKGYTEEEMLTGGIIKRGQRGGTYDFFHGRVMFPIIDLRGNVIAFGGRRMGD
                     EGGPKYLNSGDTPVFKKSRNMFALNIAKRSQSKRYILCEGYMDAIALHQAGFDTAVAP
                     LGTAFTPEQAKLLSTYADELVLCYDSDEAGQKATRRAIGILANEPVKVSILTVQGAKD
                     PDEFIKKYGPERFQMLLDGCNNAIEYELARAKANYDLTKPDGRVGYLRDAIEILSGRV
                     TPTERDVYAGRLAEETDVEKKTVLTQLEARLRAKSRKAAKEREKALLGEGAAAQIKVP
                     YTQGGQKALGVAFAEQQLMAAALKDPEHLKVIAARLTPERFITPEMAEVFQLLLQKRQ
                     RGEYVDFTQLSGELPEATVSLLSRILAQNYDVGFTERDVALYMERIEESVPKSAAAAG
                     MSPQELEGYLEKLKQKRR"
     gene            1466..>1888
                     /locus_tag="ACZPFK_12215"
     CDS             1466..>1888
                     /locus_tag="ACZPFK_12215"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HIR1407114.1"
                     /translation="MADKKTVIRELIETGKKNGKLTTKEISDAMEEIGFDVDQVDKLY
                     ETLDAHNIEVVDEPDPEIEEFKLTEENTDEFEGALAAEGVAIDAILFGGRRATNVPLV
                     AEQYENAHGVFIGSAVASEVTAAALDAKVGSLRHDPMAM"
BASE COUNT          401 a          584 c          624 g          279 t
ORIGIN      
        1 cgcccgtttc tactttgagc agctcaacac cgacgagggg cgggcggcac ggggttatct
       61 gcgcggccgc gctttgtccg acggcaccat caagcggttt gggctgggct attcgcccga
      121 gggctatcat gccaccagtg attatctgca caaaaagggc tataccgagg aggagatgct
      181 caccgggggc atcatcaagc gcggccagcg cggcggcacc tatgactttt tccacggccg
      241 ggtgatgttc cccatcatcg acctgcgcgg caacgtcatc gctttcggcg gccggcgcat
      301 gggggacgag ggcgggccca aatacctcaa cagcggcgac acgccggttt ttaaaaagag
      361 ccgcaacatg ttcgccctca atatcgccaa gcgctctcag agcaagcggt atatcctctg
      421 cgagggctat atggacgcca tcgccctgca ccaggcgggc tttgatacgg cggtggcacc
      481 gctgggtacc gcgttcacac ccgaacaggc aaagctgctg tccacctatg ccgacgagct
      541 ggtgctctgc tatgacagcg atgaggcggg gcagaaagcc acccgccggg ccatcggcat
      601 cctggccaac gagccggtca aggtctccat cctgaccgtg cagggggcca aggacccgga
      661 cgagttcatc aaaaaatacg ggcccgagcg ctttcagatg ctgctggacg gctgcaacaa
      721 cgccatcgaa tacgagctgg cccgggcgaa agcaaactat gacctcacca aacccgacgg
      781 ccgggtgggc tacctgaggg acgccattga gatcctctcg ggcagggtga cccccaccga
      841 gcgggatgtc tatgcgggcc gccttgccga ggagaccgac gtggagaaaa agacggtgct
      901 cacccagctg gaggcgcgcc tgcgggccaa gagccgcaag gcggccaaag agcgggaaaa
      961 ggcgctgttg ggcgaggggg cggcggccca gatcaaggtg ccctataccc agggcggcca
     1021 gaaagcgctg ggggtggcct ttgccgagca gcagctgatg gccgcggccc tcaaggaccc
     1081 cgagcacctc aaggtcatcg ccgcgcgcct gacgccggag cggtttatca ccccggaaat
     1141 ggccgaggtc tttcagctgc tgctgcaaaa gcggcagcgg ggcgagtacg tcgatttcac
     1201 ccagctttcc ggcgagctgc cggaggcgac ggtgtcgctg ctctcccgca tcctggccca
     1261 gaactacgac gtgggcttta ccgagcggga tgtggcgctg tatatggagc gcatcgagga
     1321 gagcgtgccg aagtccgccg ccgcggcggg catgagcccg caggagctgg agggatatct
     1381 ggaaaagctc aagcagaagc gcagataaaa agtttacgag acgacccggc gcaccgggga
     1441 agataagcaa aggggaatgc acactatggc tgacaaaaag accgtgatcc gggaactgat
     1501 cgagaccggc aaaaagaacg gcaagctgac caccaaggag atcagcgacg cgatggagga
     1561 gatcggcttc gacgtcgatc aggtggacaa gctgtacgag acgctggacg cccacaacat
     1621 cgaggtcgtg gacgagccgg acccggagat cgaggagttc aagctgaccg aggagaacac
     1681 cgacgagttc gagggcgccc tcgccgccga gggcgtggcc atcgacgcca tcctcttcgg
     1741 cggtcgccgc gccaccaacg tgccgctggt cgccgagcag tacgagaacg cccacggcgt
     1801 gttcatcggc tccgccgtgg cctctgaggt caccgccgcc gccctggacg ccaaggtcgg
     1861 ctccctgcgc cacgacccca tggctatg
//
LOCUS       DBMDYA010000588         1882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12422_length_1882_cov_1.962381, whole genome shotgun sequence.
ACCESSION   DBMDYA010000588 DBMDYA010000000
VERSION     DBMDYA010000588.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_1882_cov_1.962381"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            136..1437
                     /locus_tag="ACZPFK_12220"
     CDS             136..1437
                     /locus_tag="ACZPFK_12220"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1407115.1"
                     /translation="MRMKKPVAALAALALAVSLCAAPVMAADTAGWTDGDAGTTPTLT
                     LVTASDNSLYFFAGGTAITISAPDSPDMGAKISWDGGSLEVPANVNVFGGYHNNNTQT
                     DTSITMAGGAVHNVIGGGLHASHVGTANIVMSGGTATSVQGGGASSLTKTCGCPNGTS
                     WYAGNAADSPCRVDHANVLIDGGNVTSTLFGGGEGISSTGEAVVTVKSGSVNYLIAGG
                     SNGYTGSGSASVSGGTVAVLQGVNRGSMETIDLAVSGGTVTNVYAGGETTDSSVTGTF
                     TSAKVSVTGGAVTNLSAGTNGGQGAVEAAKDAAEITFTPGTVANTQDVPTIYHVKFLN
                     EGAVVQETNSSDPAVTAPAAPTRDGYVFKGWKGADGTLYEAGKVLEVSGDATFDAVWT
                     AQPAPTPTATPAPTATPAPTAAPSGGGSAPAKANPNTGVKL"
     gene            1588..>1882
                     /locus_tag="ACZPFK_12225"
     CDS             1588..>1882
                     /locus_tag="ACZPFK_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407116.1"
                     /translation="MKKEDKKKWLWAAGLGAVLLTAVVCLFVIPKSGPGAASSAIPSS
                     APAPTAAASPTPLALARAKNSDVVGWLSVPGTDIDDPVVQAADNEHYLRRTWEG"
BASE COUNT          358 a          667 c          595 g          262 t
ORIGIN      
        1 gctacaatta ccattaatgt ggggattttg tgaaaagcct gcatggatac cacataatgc
       61 agattatgtg gtgcgccccc ttgaaaaaac gcggggcggg aagcctgaaa ccatattttg
      121 ttggaggtaa ttggaatgag gatgaaaaag cccgtggcgg ccctggccgc ccttgccctg
      181 gccgtgtcgc tgtgcgccgc gccggtgatg gccgccgaca cggccggctg gaccgacggc
      241 gacgcgggga cgaccccgac cctgaccctg gtcaccgctt cggacaactc gctttacttt
      301 tttgcgggcg gcacagccat cactatttct gcgccggaca gcccggacat gggcgccaag
      361 atcagctggg acggcggcag cctggaggtg cccgcaaacg tcaatgtctt tggcggctac
      421 cacaacaaca acacccagac cgacaccagc atcaccatgg ccggcggcgc cgtgcacaat
      481 gtgatcggcg gcggcctgca cgcaagccat gtgggcaccg ccaacatcgt gatgagcggc
      541 ggcacggcga cctcggtgca gggcggcggc gcctcctccc tgaccaaaac ctgcggctgc
      601 cccaacggca caagctggta cgcgggcaac gcggccgatt ccccctgccg ggtggaccac
      661 gccaatgtcc tgattgacgg cggcaacgtc acctccacgc tgttcggcgg cggcgagggg
      721 atcagctcca ccggcgaagc cgtggtgacc gtcaagagcg gcagcgtcaa ctacctcatc
      781 gcgggcggct ccaacggcta caccggcagc ggctctgcat ccgtgagcgg cggcaccgtg
      841 gcggtgctgc agggcgtcaa ccgcggcagc atggagacca tcgacctggc cgtctccggc
      901 ggcacggtga ccaatgtgta cgcgggcggc gagaccaccg actccagcgt caccggcact
      961 ttcaccagcg ccaaggtctc ggtcaccggc ggcgcggtca ccaacctgtc cgctggcacc
     1021 aacggcggcc aaggcgctgt ggaagcggcc aaggacgcgg ccgagatcac ctttacgccc
     1081 ggcaccgtcg ccaacaccca ggacgtcccc accatctacc atgtaaaatt tctcaacgag
     1141 ggcgcggtcg tgcaggagac caactcctct gatcccgcgg tgaccgcccc cgcggccccc
     1201 acccgcgacg gatacgtctt caagggctgg aaaggcgccg acggcaccct gtacgaggcg
     1261 ggcaaggtgc tggaggtgag cggcgacgcc acctttgacg cggtctggac cgcccagccg
     1321 gccccgacgc ccacagccac cccggccccg acggcgaccc ctgcgcccac cgccgccccc
     1381 tccggcggcg gctcggcccc ggccaaagcg aaccccaaca ccggcgtaaa actgtaatca
     1441 caaggccccg ccgccaagcg ggcgccaaag agcggacgac acgggagcgg aattttccgc
     1501 tcccgtgtcg tttcaccgtc gcaaagcgct tttgccgcag cgctccggca gccgcggcgg
     1561 cttcttcgac ggtcaaagga ggaacagatg aaaaaagagg ataaaaagaa atggctatgg
     1621 gccgccgggc tgggcgcagt gctgctgacc gccgtggtct gcctgttcgt catccccaaa
     1681 tccgggcccg gagccgcatc ctcagcgatc ccctcgtccg ccccggcccc aacggccgcg
     1741 gccagtccca cgccgctggc gctggcccgg gcgaaaaaca gcgacgtggt gggctggctc
     1801 tcggtgcccg gcaccgacat cgacgatccg gtggtacagg ccgccgacaa cgagcactac
     1861 ctgcgccgca cctgggaggg ca
//
LOCUS       DBMDYA010000589         1881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12427_length_1881_cov_1.297753, whole genome shotgun sequence.
ACCESSION   DBMDYA010000589 DBMDYA010000000
VERSION     DBMDYA010000589.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12427_length_1881_cov_1.297753"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="ACZPFK_12230"
     CDS             complement(<1..240)
                     /locus_tag="ACZPFK_12230"
                     /inference="COORDINATES: protein motif:HMM:NF045043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin-like domain-containing protein"
                     /protein_id="HIR1407117.1"
                     /translation="MEQMRWRCTVCGEIFEGEAPPAPCPVCGAGADAFEPVAAPAVRR
                     WKCTVCGQVFEGEVPPVPCPVCGAGADAFVPLEEKT"
     gene            complement(345..458)
                     /locus_tag="ACZPFK_12235"
     CDS             complement(345..458)
                     /locus_tag="ACZPFK_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin-like domain-containing protein"
                     /protein_id="HIR1407118.1"
                     /translation="MKKWKCTVCGEIFEAETKPSECPVCGVGPEMIEEVTE"
     gene            632..1294
                     /locus_tag="ACZPFK_12240"
     CDS             632..1294
                     /locus_tag="ACZPFK_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1407119.1"
                     /translation="MEKSLQALHTCPLFEGICDQELEQLLPCLHARRRSCPRGAVLLR
                     EGERTGELGVVLTGLLTLEKTDRGGNLNLIAQAGPGELFGDPAACAAGGGSGVSVRCA
                     QAAQVLLVELDQVTKRCSRACAFHSRLVQNLLRLLGQGSLSLEKKIEVLSQRTIREKL
                     LCCLQAERARAGSPRFTLPLSRSGLADYICADRSAMTRELGRMQKEGLLELRGRAVYL
                     PE"
BASE COUNT          304 a          634 c          613 g          330 t
ORIGIN      
        1 cgtcttttct tccagcggga caaaagcatc cgcccctgcc ccacagaccg ggcaggggac
       61 tggcggcacc tcgccctcaa agacctgccc gcagaccgtg cacttccagc gccgcaccgc
      121 gggggccgcc accggctcaa aggcgtccgc ccccgccccg cagaccgggc agggggcggg
      181 cggggcctcg ccctcaaaaa tctcgccgca cacggtgcag cgccatctca tctgttccat
      241 cagcagcctc cttcagcgct tgcgcaagca gaaaacgggc cggaaagcca agccgggctg
      301 cgcccggcca ctttccggcc cgcctcattg ccgccgtacc ttttttactc ggtgacttct
      361 tcgatcatct cggggcccac gccgcagacg gggcactcgg acggcttggt ttccgcctca
      421 aagatctcac cgcagaccgt gcatttccac tttttcatga gagaagatct ccttttgcaa
      481 tacagagctt gatctaaggg tcaacgccgg gccggcggtg ccggagcctg gctctcacca
      541 ttttgtgaga gacgcagctt gcgtcttttt aaatatgata ctatactgga cagaaaaaat
      601 ctgttgcatt tgcaacgcgg agagggaaat catggagaaa agtttacagg ccttacatac
      661 ttgcccgctg tttgagggga tctgtgacca agagctggag cagctgctgc cctgcctgca
      721 cgcccggcgg cgcagctgcc cgcggggggc ggtgctgctg cgggagggcg agcgcaccgg
      781 cgagcttggg gtggtgctga cggggctact cacgctggaa aagaccgacc gcggcggcaa
      841 cctgaacctg atcgcccagg cgggcccggg ggagctgttc ggggaccccg cggcctgcgc
      901 ggccggcggc ggcagcggcg tgtcggtgcg ctgcgcccag gccgcccagg tgctgctggt
      961 ggagctggac caggtgacca agcgctgcag ccgcgcctgc gcgttccatt cccgcctagt
     1021 gcagaacctg ctgcggctgc tggggcaggg cagcctttcg ctggagaaaa agatcgaggt
     1081 gctcagccag cgcaccatcc gcgaaaagct gctctgctgc ctgcaggccg aacgggcgcg
     1141 ggccggctcc ccgcgcttca cgctgccgct ctcccgcagc gggctggccg actacatctg
     1201 cgccgaccgc agcgccatga cccgcgagct ggggcgcatg cagaaagagg gcctgctgga
     1261 gctgcgcggc cgggcggtct atctgccgga gtgagccgcc ctctgcgcgc aaaaaagcca
     1321 aaggcccggc gcgccgcctt accctagcgt tgaccggcgg gcggccggtt atgcgccggg
     1381 cggcggatct gcgccgccgc agccccgaaa aatgaaaggt taccccgggc ccgcggcccg
     1441 gcggcggcgg caaagcaggg gcgaggggtg tgaaatttgt gtgaggcgct tgccaacttg
     1501 tgccaccgct ccctctggcc gcaggacgga ggcctgccgc tctgtgcggc cggatactgc
     1561 tgttttcgcc gctttgcggc ggtttgcgcc cactgcggcg accccggccc ggcggggaag
     1621 cgaaagccgt ctcaaaccgc cccaaaccgc ggccccggtt gatttttcgc cctttttagt
     1681 gtactatatt tgtatctatc gggccgcggc gggtcagccc cgcctttgac gggagggatc
     1741 ggacgcgtcc tcccggcggc ctctgttgaa aggagctctc cctatggcta tgatcgcccc
     1801 ctccctgctc tctgccgact tcgcgtgcct ggagcaggat tgccgcacct gccttgacgc
     1861 gggagcggat tttttacatt t
//
LOCUS       DBMDYA010000590         1879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12440_length_1879_cov_1.260967, whole genome shotgun sequence.
ACCESSION   DBMDYA010000590 DBMDYA010000000
VERSION     DBMDYA010000590.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12440_length_1879_cov_1.260967"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="ACZPFK_12245"
     CDS             <1..282
                     /locus_tag="ACZPFK_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407120.1"
                     /translation="EDEAARRRERGRLLRGPARAQRQVRAALEGRDDVFDITRDGVSL
                     SPGLFHACFSVWRREPLEDNPHAGALLTACWGGVRFFTYAAPGTEARDG"
     gene            275..469
                     /locus_tag="ACZPFK_12250"
     CDS             275..469
                     /locus_tag="ACZPFK_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407121.1"
                     /translation="MDEGLGLFAPRRFYRCPICGAVLDSDDDRVLVDRDGDPANPADV
                     AGCSRCLRALRAERMEWYLD"
     gene            513..1430
                     /locus_tag="ACZPFK_12255"
     CDS             513..1430
                     /locus_tag="ACZPFK_12255"
                     /inference="COORDINATES: protein motif:HMM:NF039403.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6291 domain-containing protein"
                     /protein_id="HIR1407122.1"
                     /translation="MAREYFNAYHSYLDSMEFLSDAERGRLFTALLLYSSTGEAPQLR
                     GNERFVFPGIRSQIDRDIGNYDAYCARQRANGSKGGRPRKAPPRPEPDGFAANPKKPG
                     EREGKGESKGEEKGEGEPERTSSSPPGAEEAEEAVRFWEENFGPMPRCLRQAAEGYLR
                     RGLEGALVTWALGEAAKTGAHQPWRYAETILKDCLAQGLHTLAEYERARRGWGRNARP
                     PEREIRPEQDLLQLAGDKLPVFRKLPGREKDGPGEGNRANPGAERAGGRTQESGADGG
                     AGRGDENLGGVVPDRENRTEPDPGLGALG"
BASE COUNT          323 a          573 c          776 g          207 t
ORIGIN      
        1 gaagacgagg ccgcgcggcg gcgcgagcgg gggcggctgc tgcgcggccc ggcccgggcg
       61 cagcggcagg tgcgcgcggc gctggagggc cgcgacgacg tgttcgacat cacccgcgac
      121 ggcgtcagcc tgtcccccgg gctgttccat gcctgctttt ccgtctggcg gcgggagccg
      181 ctggaggata acccccacgc cggcgcgctg ctgaccgcct gctggggcgg ggtgcggttt
      241 tttacctatg cggcccccgg cacggaggcc cgcgatggat gagggcttgg ggctgttcgc
      301 gccccggcgg ttttaccgct gccccatctg cggcgcggtg ctggacagcg acgacgaccg
      361 ggtgctggtg gaccgggacg gggacccggc caacccggcc gacgtggcgg gctgcagccg
      421 ctgcctgcgg gcgctgcggg ccgagaggat ggaatggtac ctggattaaa aagggccgcg
      481 gcgcggcccg ctttttggag gaggacgaag cggtggcaag agagtatttc aacgcctatc
      541 acagctatct ggactcgatg gagttcctca gcgacgccga gcgggggcgc cttttcacgg
      601 cgctgctgct ctacagcagc acgggcgagg cgccgcagct gcgcggcaac gagcggtttg
      661 tgtttcccgg catccgctcg cagatcgacc gggacatcgg caactacgac gcctactgcg
      721 cccggcagcg ggccaacggc agcaagggcg gccgcccccg caaggcgccg ccccggccgg
      781 aaccggacgg ttttgcggca aacccgaaaa agcccggaga aagagaggga aaaggcgaaa
      841 gcaaaggcga agaaaaagga gagggagaac cggagaggac ttcgtcctct ccgcccgggg
      901 cggaggaggc ggaggaagcc gtccgctttt gggaagaaaa ttttgggccc atgccccgct
      961 gtctgcgcca ggcggccgag ggctacctgc gccgggggct ggagggggcg ctggtgacct
     1021 gggccctcgg agaggcggcc aagaccgggg cgcaccagcc ctggcggtat gcggagacga
     1081 tcctgaagga ctgcctggcc cagggcctgc acaccctggc cgagtatgag cgggcccgcc
     1141 gcggctgggg cagaaacgcc cggcccccgg agcgggagat ccgcccggag caggatctgc
     1201 tgcagctggc gggggacaag ctgccggtgt tccgcaagct gccgggcagg gagaaggacg
     1261 gcccgggaga ggggaaccgg gcgaacccgg gcgcggagcg cgcgggcggc cggacgcaag
     1321 agagcggcgc ggacgggggc gcggggcgcg gggatgagaa cctcggggga gttgtcccgg
     1381 acagggaaaa ccgcacagag ccggacccgg gccttggggc actggggtga cccccgcggg
     1441 cactgccccg gacagagaga gcggcgcaga cgggggcctg gggatgagaa cctcgaggga
     1501 gttgtcccgg acggggaaaa ccgcgcagag ccgggcccgg gccttgaggc acgggggtga
     1561 ccccctggcg ggattgccca gacagggaga acagcgcaga ggggggcctg ggcgcggggc
     1621 acggggatga gaaccttggg ggaattgtcc cggacgggga aaaccgccca gagccggacc
     1681 cgggccttga ggtacggggg tgacccccgc ggggactgcc caggacagag agagaggcgc
     1741 ggacggaggc ctggggcgcg gggatgagga ccttggggga attgtcccgg acggggaaaa
     1801 ccgcacagag ccgggcccgg gccttgaggc acgggggtga tccccacggg gactgccccg
     1861 gacagagaga gcggcgcag
//
LOCUS       DBMDYA010000591         1878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12446_length_1878_cov_1.258863, whole genome shotgun sequence.
ACCESSION   DBMDYA010000591 DBMDYA010000000
VERSION     DBMDYA010000591.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12446_length_1878_cov_1.258863"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /gene="secY"
                     /locus_tag="ACZPFK_12260"
     CDS             <1..1195
                     /gene="secY"
                     /locus_tag="ACZPFK_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1407123.1"
                     /translation="TGGAFAQATLFALSVTPYINASIIIQLLTVALPPLERLAQEGEE
                     GRRKINRITRYTGAGIALALAVAYFALLKSRGAVQYTEGWQGWFTAVVIVLAFTAGSM
                     LIMWLGEQIDKKGIGNGISLLIFAGIVSRLGTMPATIYNYIAGIVNGFKAGGDLTQIS
                     LGGQTMYITAEGTAAYPWYYILYLLLILVLMIASVIFVVVMTSGERRIPVQYAKRVVG
                     RKMYGGQATHIPIKVNMSGVMPVIFASSLISIPGTIKSIFNIQGTGFWATFLGWFNYN
                     GLAYALIYALLILAFNYFYVAIQYNPVEIANNLRKNNGAIPGIRPGKPTTDFIVKVLG
                     KITFIGAVFLMVIALMPIIVGNLTHINIQLGGTSLLIVVGVALDTARQLESFMLMRHH
                     KGFLE"
     gene            1212..1844
                     /locus_tag="ACZPFK_12265"
     CDS             1212..1844
                     /locus_tag="ACZPFK_12265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1407124.1"
                     /translation="MNLILLGAPGAGKGTQAEIICEKFSIPAVSTGNILREAVKNGTE
                     MGKKAKAYIDGGKLVPDDVIISIIKERLSEPDCENGFILDGVPRTVVQAEAIEKAGIR
                     IDKVIDIEVADGDIVKRLSGRRVCASCGASYHTVYKPSTDPSKCDRCGGELIVRKDDE
                     PATVLERLAVYHEQTEPLKDFYAARGKLAVVEGQEEVADTTALVLKALEA"
BASE COUNT          371 a          614 c          546 g          347 t
ORIGIN      
        1 caccggcggc gcgttcgccc aggcaaccct gttcgccctt tcggtcaccc cctacatcaa
       61 cgcctccatc atcatccagc tgctcaccgt ggcgcttccg ccgctggaac ggctggcgca
      121 ggagggcgaa gagggccgcc ggaagatcaa ccgcatcacc cgctacaccg gcgcgggcat
      181 cgccctggct ttggccgtgg cctatttcgc cctgctgaaa agccgcggcg cggtgcagta
      241 caccgagggc tggcagggct ggttcaccgc cgtggtcatc gtgcttgctt tcaccgccgg
      301 ctcgatgctc atcatgtggc tgggcgagca gatcgataaa aagggcatcg gcaacggcat
      361 ctcgctgctg attttcgcgg gcatcgtctc gcgtctgggc accatgcccg ccacgatcta
      421 caactatatc gcgggcatcg tcaacggctt caaggccggc ggcgatctga cccagatcag
      481 cctgggcggc cagacgatgt atatcaccgc ggagggcacc gctgcctatc cctggtacta
      541 catcctctat ctgctgctca tcctggtgct gatgatcgcc agcgtgatct ttgtggtcgt
      601 catgaccagc ggcgagcgcc gcatcccggt gcagtacgcc aagcgcgtgg tgggccgcaa
      661 gatgtacggc ggccaggcca cccacatccc catcaaggtc aacatgagcg gcgtcatgcc
      721 ggtcatcttc gcctcgtctc tgatctccat ccccggcacc atcaagtcga tctttaatat
      781 ccagggcacc ggcttctggg cgactttcct gggatggttc aactacaacg ggctggctta
      841 tgcgctcatc tacgcgctgc tgatcctggc gttcaactat ttctacgtcg ccatccagta
      901 caatcccgtc gagatcgcca acaacctgcg caagaacaac ggcgccatcc cgggcatccg
      961 tcccggcaag ccgaccactg attttattgt gaaggttttg ggcaaaatta cctttatcgg
     1021 cgcggtcttc ctgatggtca tcgcgctgat gccgatcatc gtgggcaacc tcacccatat
     1081 caatatccag ctgggcggca cctcgctgct catcgtggtg ggcgtcgcgc tcgacaccgc
     1141 ccgtcagctt gaaagcttta tgctgatgcg gcatcacaag ggcttcttag agtaaccagg
     1201 agggagacaa catgaatctg atccttttgg gcgcaccggg cgccggcaaa ggcacccagg
     1261 cagaaatcat ctgtgaaaag ttctccatcc ccgccgtcag caccggcaac atcctgcggg
     1321 aggccgtgaa aaacggcacc gagatgggca aaaaagccaa ggcctacatc gacgggggca
     1381 agctggtgcc ggacgacgtg atcatcagca tcatcaaaga gcgcctctca gagcccgatt
     1441 gcgagaacgg ctttatcctg gacggcgtgc cccgcacggt cgtccaggcc gaggccatcg
     1501 aaaaggcggg catccgcatc gacaaggtca tcgacatcga ggtggcggac ggcgatatcg
     1561 tcaagcgttt gtccgggcgg cgcgtctgcg cctcctgcgg cgccagctac cacaccgtct
     1621 acaagccctc gaccgacccc agcaagtgcg accgctgcgg cggggagctg atcgtccgca
     1681 aggacgacga gcccgccacc gtgctggagc gtctggcggt ctatcacgag cagacggaac
     1741 ccctgaaaga cttctacgcg gcccgcggca agctggccgt cgtcgagggg caagaggagg
     1801 tcgcggacac caccgccctc gtcctcaaag ccctggaggc gtgagcgtga tctcgataaa
     1861 gacttccaga gagatcga
//
LOCUS       DBMDYA010000592         1876 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12461_length_1876_cov_1.778028, whole genome shotgun sequence.
ACCESSION   DBMDYA010000592 DBMDYA010000000
VERSION     DBMDYA010000592.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12461_length_1876_cov_1.778028"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="ACZPFK_12270"
     CDS             <1..859
                     /locus_tag="ACZPFK_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012472.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1407125.1"
                     /translation="EDLLTRLQTDYIDLGMIHFIDSEEEYRAIFETGLIDQILELKKQ
                     GKIRHLGFSSHDPQVALKVVRTGLVEAMLFSINAAYDLLPPEVKLPCKLDADSFTRDG
                     TAAFNPARAALYDACAAAGVGITVMKTLGAGRLLSADSSPFGVALSVPQCIQYVLSRP
                     AVASAMVGMQTVEEVEQALAFEDATEEERDFSGIFAAASQFTMAGKCMYCNHCLPCPQ
                     GLDIAAIHKYLDLATAAEAVPDTVRAHYEALDRHAGDCIGCRGCESRCPFDVKTVEAM
                     AEAKALFGY"
     gene            985..>1876
                     /locus_tag="ACZPFK_12275"
     CDS             985..>1876
                     /locus_tag="ACZPFK_12275"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1407126.1"
                     /translation="MNKLDFDNPIRQQTFSLTDLVEAQLEGCFGPGLKELMSMAEIFD
                     ARKIILTGCGDSYGAAIAMAPVIAKYCDCFGVQAMRTVEFTRFLSKADIGIGEPNSPL
                     VIAISAGGGTARICEALEKANQVGAFSILLTNNGESRAAKAAKRVYVVGTPPFPNDHP
                     GLRSYFGSLVGLIAFASRMGHVRGTLPPTGPAQFKKAITDYVKSYEGVMERIDDQMFA
                     LAQRWQAFERFDFIGDDVEYGSAFFGAAKFLECNGCTVSVDDSEDWCHINYFIKNPET
                     IGTVFMADRHRWLQWPDGYRT"
BASE COUNT          367 a          561 c          585 g          363 t
ORIGIN      
        1 tgaggatctt ttgaccaggc tgcagaccga ctatatcgac cttggcatga tccactttat
       61 cgacagcgag gaggagtatc gggcgatctt tgagaccgga ctcatcgacc agatcctgga
      121 gctgaaaaag cagggcaaga tccgccatct gggcttcagc tcccacgacc cgcaggtcgc
      181 gctgaaagtg gtgcgcaccg gcctggtgga ggcaatgctg ttcagcatca acgccgcata
      241 cgatctgttg cccccggagg tgaagctgcc ctgcaagctg gacgcggaca gcttcacgcg
      301 ggacggcacc gcggcgttca acccggcccg ggcggcgctc tacgacgcct gcgcggcggc
      361 gggggtgggg atcacggtca tgaagaccct gggcgcgggc cgtctgctga gcgccgacag
      421 ctcgccgttc ggcgtggccc tctcggtccc ccagtgcatc caatacgtgc tcagccggcc
      481 cgcggtggca agcgccatgg tgggcatgca gacggtggaa gaggtggagc aggccctggc
      541 ttttgaggac gccacagagg aagaacggga tttttccggt atcttcgcgg ccgcgtccca
      601 gttcaccatg gcgggcaaat gtatgtactg caaccactgc ctgccctgcc cgcagggcct
      661 ggatatcgcg gccatccaca agtatctgga cctggccaca gcggcagaag ctgtgcccga
      721 caccgtgcgc gcccactatg aggccctgga ccgccatgcg ggggactgta tcggctgccg
      781 cggctgcgag agccgttgcc cctttgatgt gaaaacggta gaggccatgg cagaggcaaa
      841 agcgctgttc ggatactaaa aagtcaaaat aagtttaaat aaagttcaga cattgcttac
      901 aaaatgaatt ttttatggta taattttgaa agcggaggcc tttgtgctcc ccattttatg
      961 ttttgcgtta ggaggtagtt ctgagtgaat aagctggact tcgacaaccc catccgccag
     1021 cagacgttca gcctcaccga tctggtggag gcgcagcttg agggctgttt tggcccgggc
     1081 ctgaaagagc tgatgagcat ggcggagatc ttcgacgccc gcaagatcat cctcaccggg
     1141 tgcggagact cttacggcgc ggccatcgcc atggcgccgg tgatcgccaa atattgtgat
     1201 tgcttcggcg tgcaggcgat gcgcacggtg gagttcaccc gctttttgtc caaggcggac
     1261 atcggcatcg gcgagccgaa cagcccgctg gtgatcgcca tcagcgccgg cggcggcacg
     1321 gcccgcatct gcgaggcgct ggaaaaagcg aaccaggtgg gcgctttctc gatcctgctc
     1381 accaacaacg gcgagtcccg cgccgcaaag gcggcaaagc gggtctatgt ggtgggcact
     1441 ccgccgttcc ccaacgacca cccgggcctg cgctcttatt tcggttccct ggtggggctg
     1501 atcgcctttg cctcccgcat gggtcatgtg cgcggcaccc tgccccccac cgggcccgcc
     1561 cagttcaaaa aagccatcac cgattatgtc aagagctatg agggcgtgat ggagcgcatc
     1621 gacgaccaga tgttcgcgct ggcccagcgc tggcaggcct ttgagcgctt tgacttcatc
     1681 ggcgacgatg tggagtacgg ctccgccttt ttcggggcgg ccaagttcct cgagtgcaac
     1741 ggctgcaccg tgtcggtgga cgactcggag gactggtgcc acatcaacta cttcatcaag
     1801 aaccccgaga ccatcggcac cgtctttatg gccgaccgcc atcgttggtt acaatggccg
     1861 gacgggtaca gaacat
//
LOCUS       DBMDYA010000593         1872 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12480_length_1872_cov_1.693958, whole genome shotgun sequence.
ACCESSION   DBMDYA010000593 DBMDYA010000000
VERSION     DBMDYA010000593.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_1872_cov_1.693958"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="ACZPFK_12280"
     CDS             <1..1446
                     /locus_tag="ACZPFK_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407127.1"
                     /translation="HGLLGENGAGKSTFVKIISGLLAPDGGVFELMGEPVAPKSPVEA
                     ARLGIVKLQKRSGLVPSLTVEENLLLGRQKNSFSPLDRKKMRREVAALLKEFEMDPGP
                     GDLALHLTAEDALETEVLKAYYAGAHLIILDEPASYFSLAQTEELFTLLRRVAAKGVS
                     ILYVSNQVREMLELCDRITVMEGGITTATLAAAETSPQQLQRRMVQENKAPKVRKTRA
                     KPGDTVLKVRGLSTFRNAQRPVLNNVSFSVRAGEILAVVGKPDSGTRELCEVVAGLDS
                     AVAGQVLVFDDDITGDSVRGVRDLGVSFLISTPQRIGVAPALSIQENLLPYQYTNPAY
                     RSHGLLDKEKLKAEADRLIGEYDIRCAGGWENAAVLSTSSAQKLLAAREFSNEPVLLV
                     AYQPTFGTSEETAAFLEKKLIELRNEGTAILLVPTDWDEFATLADSALVLDKGQVSAY
                     LPKVQGGEVLDPYINGESRMSDEELEAVCFE"
     gene            1439..>1872
                     /locus_tag="ACZPFK_12285"
     CDS             1439..>1872
                     /locus_tag="ACZPFK_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1407128.1"
                     /translation="MNKFNEKFPPSAQRRLTACLAGLAFCLLAILLVEPAKGEALKTF
                     FIGPFSSLSSVGGLISAATPAVFLALGVSVVFQCRRYSFAVIGSFLLSSGLVTMFLLR
                     GNEKPGWYLVPTALLIAVAAGAAVAVIPAWLAVRRRVNVTLSS"
BASE COUNT          318 a          570 c          648 g          336 t
ORIGIN      
        1 cacggcctgc tgggggagaa tggggcgggc aaatccacat ttgtcaagat catcagcggg
       61 ctgctggcgc cggacggcgg cgtctttgag ctgatgggcg agcccgtcgc acccaagagc
      121 ccggtggagg cggcgaggct cggcatcgtc aagctgcaaa agcgctccgg cttggtgccc
      181 tcgctgacgg tggaagaaaa tctgctgctg ggcaggcaga aaaacagctt ttctccgctg
      241 gaccgcaaaa agatgcggcg ggaggtcgcc gcgctgctga aagaattcga aatggacccg
      301 ggccccggcg acctggccct gcacctcacc gcagaggacg cgttggagac cgaggtgctc
      361 aaggcgtatt acgcgggtgc gcatctgatc atcctggacg agcccgcctc ctatttcagc
      421 ctggcgcaga ccgaagagct gttcacgctg ctgcgccggg tcgccgcaaa gggcgtctcg
      481 atcctctacg tctccaacca ggtgcgggag atgctggagc tgtgcgacag aatcacggtg
      541 atggagggcg gcataaccac ggccaccctc gccgcggcgg agaccagccc gcagcagctg
      601 cagcgccgca tggtgcagga gaacaaggcg cccaaggtgc gcaagacccg cgccaagccc
      661 ggcgacacgg tgctcaaggt gcgggggctg tccacctttc ggaacgccca gcgcccggtg
      721 ctgaacaatg tgagcttttc ggtgcgggcg ggggagatcc tggccgtggt gggcaagccg
      781 gacagcggca cgcgggagct gtgcgaggtc gtggcgggcc tcgacagcgc cgtggccggc
      841 caggtgctgg tgtttgacga cgacatcacc ggcgacagcg tgcgcggcgt gcgggacctg
      901 ggggtctcct ttttgatctc caccccgcag cgcatcggtg tggcgccggc gctgagtatc
      961 caggagaacc ttctgcccta tcagtacacc aaccccgctt accgcagcca tgggctgctg
     1021 gacaaggaaa agctgaaagc ggaggcggac cggctgatcg gggaatatga tatccgctgc
     1081 gcgggcggct gggagaacgc cgccgtgctc tccacctcct cggcgcaaaa gctgctggcg
     1141 gcgcgggagt tctccaacga gccggtgctg ttggtggcct atcagcccac ctttggcacc
     1201 agcgaggaga cagcggcttt tttggagaaa aagctgatcg agctgcgaaa cgaggggacg
     1261 gcgatcttgc tggtccctac cgactgggac gaatttgcga ccctggccga cagcgccctg
     1321 gtgctggaca agggtcaggt gagcgcctat ctgcccaagg tgcagggcgg ggaagtgctg
     1381 gacccctata tcaacggcga gagccgcatg agcgacgagg aattggaggc ggtgtgcttt
     1441 gaataaattc aacgaaaagt ttccgcccag cgcccagcgc cgtctgaccg cctgcctggc
     1501 ggggctcgcc ttttgcctgt tggcgatcct tttggtggag cccgcaaagg gcgaggcgct
     1561 gaagaccttt ttcatcgggc ccttttcctc tctctcctcc gttggcggcc tgatttcggc
     1621 ggccaccccc gcggtctttc tcgccctggg ggtgtcggtg gtgtttcagt gccggcgcta
     1681 cagctttgca gtgatcgggt cttttctgct ctcctccggc cttgtcacga tgtttttgct
     1741 gcgcggcaac gaaaagccgg gctggtatct ggtgccgacg gccctgctga tcgcggtggc
     1801 ggcgggggcc gccgtggcgg tgatccccgc ctggctggca gtgcgccgca gggtgaacgt
     1861 caccctctcc tc
//
LOCUS       DBMDYA010000594         1872 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12481_length_1872_cov_1.591756, whole genome shotgun sequence.
ACCESSION   DBMDYA010000594 DBMDYA010000000
VERSION     DBMDYA010000594.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12481_length_1872_cov_1.591756"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="ACZPFK_12290"
     CDS             complement(<1..400)
                     /locus_tag="ACZPFK_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407129.1"
                     /translation="MMEPRGRMMLKVTGILYIVFAVLSMVGGLVAMLGGGLFALGGIG
                     ANAYLGAAMGGVVLFSGLVLILSNILGLVTGILGVRYCDKPEKAQVCFVLGVILIVFA
                     ALSLLGDLFGSASASDIFSGLVGLALPICYT"
     regulatory      743..840
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            992..>1872
                     /locus_tag="ACZPFK_12295"
     CDS             992..>1872
                     /locus_tag="ACZPFK_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR1407130.1"
                     /translation="MEKFFKLKERGTTVKTEIIAGLTTFMTMAYVLAVQPSAIVGFGP
                     DPTITDINGVVISKQAILIMCALISGIITLFMGLYANFPFALSTGMGTNFLLGAMLQS
                     GQLSFGAMMAIILISGVIFVLLSVFGIRDLIVRMIPKNIKVAISASIGFFIAYLGFKN
                     TGIGTYADGIGMGDFTQPSVILALVGLLIIAVLTAFKVKGAILYGIVAVTVLGIPFGV
                     THLPASIFEAPSFATVSNVCFNFDFKGLLSGSTLVLIFIAFFGDFFSTLGTVLGVAGK
                     AKMLDEEGNLPGIQKPFL"
BASE COUNT          359 a          643 c          479 g          391 t
ORIGIN      
        1 ccgtgtagca gatgggcagg gccaggccga cgaggccgct gaaaatgtcg ctcgccgacg
       61 cgctgccgaa gaggtccccc agcaggctta aggccgcaaa gacgatcagg atcacgccca
      121 gcacaaagca gacctgcgct ttttcaggct tgtcgcagta tctcacgccc aagataccgg
      181 tgaccaggcc caagatattg ctcaggatca gcaccagccc gctgaacagc acgacgccgc
      241 ccatggccgc gcccaggtag gcgttcgccc cgatgccccc cagggcgaac aggcccccgc
      301 ccagcatggc caccaggccc cccaccatgc tcagcaccgc aaacacgatg tacaggatcc
      361 ccgtgacctt gagcatcatt cttcctctcg gttccatcac acacagctcc ttccccaaat
      421 gggtattcag ccgtccccat gagagagggg caggcttgtt gcctccatta tagcccaccc
      481 cggcctctgc cgtcaatttc ctcaaaaggg cacgccgccc ctttccggcg cggggcggac
      541 gccgcaaaag cgccggggga cgcggccgcc gttctccgcc gccctcctgc gcccggcccc
      601 acaaaaagcg ccccaaaaaa atcccggcat accgcacaga acaagtctca agcttttgtc
      661 gattgtcccc aaattcctcc ccgctcgccc cggatatttc tacagcgtcc cgccttgctt
      721 tttccagcct catcccccat aataattatt tgtcgatagt tgttgtttaa tatccgaata
      781 gggcggatag tttctacacc tcaccgtaaa tgaggtacaa tgacaacagg cgcagctttg
      841 ctgtgccctg acgaccctgg aaagagcatt tctcctcttt gcggggctgt tttgtgctgc
      901 aaccaactgt tgacatgtcc gcgcgcctgt cacggttcgg tagccccggc gggtgcaaca
      961 cacacagctc tgcaaggagg agttttttcg tatggagaaa tttttcaagc tcaaagaacg
     1021 cggcaccacg gtcaagaccg agatcatcgc cggcctgacc acctttatga ccatggccta
     1081 tgtgctggcg gtacagccct cggccatcgt gggctttggc cccgacccca ccatcaccga
     1141 catcaacggt gtggtcatca gcaagcaggc catcctgatt atgtgcgcgc tgatctccgg
     1201 catcatcacc ctgttcatgg gcctgtacgc caacttcccg ttcgcgctgt ccaccggcat
     1261 gggcaccaac tttctgctgg gcgcgatgct gcaaagcggc cagctctctt tcggcgccat
     1321 gatggccatc atcctcatct cgggcgtcat cttcgtgctg ctcagcgtct tcggcatccg
     1381 cgatctgatc gtgcgcatga tccccaagaa catcaaggtg gccatctcgg cctccatcgg
     1441 ctttttcatc gcctatctcg gctttaaaaa caccggcatc ggcacctatg ccgacggcat
     1501 cggcatgggc gacttcaccc agccctcggt catcctggcc ctggtgggcc tgctgatcat
     1561 tgccgtgctc accgcgttca aggtgaaagg cgccatcctg tacggcatcg tcgccgtgac
     1621 cgtgctgggc atcccgttcg gcgtcaccca tctgcccgcc agcatctttg aggcgcccag
     1681 ctttgccacc gtcagcaacg tctgcttcaa cttcgacttc aagggcctgc tctccggcag
     1741 cacgctggtg ctgatcttca tcgcgttttt cggcgatttc ttctccacgc tgggcactgt
     1801 gctgggcgtt gccggcaagg ccaagatgct ggatgaggag ggcaacctgc cgggcatcca
     1861 aaagccgttt ct
//
LOCUS       DBMDYA010000595         1869 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12504_length_1869_cov_1.939480, whole genome shotgun sequence.
ACCESSION   DBMDYA010000595 DBMDYA010000000
VERSION     DBMDYA010000595.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_1869_cov_1.939480"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="ACZPFK_12300"
     CDS             complement(<1..570)
                     /locus_tag="ACZPFK_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase, subunit RnfA"
                     /protein_id="HIR1407131.1"
                     /translation="MTVLRPVLAFFVYAVVAVAAQNAIFTRALGVSRLVKMVDDGSVE
                     LLKFGALLTFVQFLSSLLGYFVNRLLAGSSFAYKAAFQPLVLVLCSVVAFFVVLLGVA
                     LLGGRIAAKDYVELLPMATFNCCVLGTLLLTTLGGYTLAQTLAFSLGTSVGYVLAVLL
                     VDEGQRKIQNRNLPATFRGLPVTLVYIGIL"
     gene            complement(567..1214)
                     /locus_tag="ACZPFK_12305"
     CDS             complement(567..1214)
                     /locus_tag="ACZPFK_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR1407132.1"
                     /translation="MKQERSLYYAENMDRLMHRDRFFFNNPVFMQGLGLAPIVVAATN
                     LNNALVLGVAVLLLLTPTRVLAALLSRGNYLRFRGVTYAVTAAVLYIGVWFAANALFG
                     VGIQAVGLYLPLLVVEPIIIKRYERPQKERVSTALRKGLITTCGFLLALFLVAALREL
                     LGSGALFGHPVAARPPLPMAQLTCGGFILIGILAALWRWMRNLFKRVVSMEANAK"
     gene            complement(1211..>1869)
                     /locus_tag="ACZPFK_12310"
     CDS             complement(1211..>1869)
                     /locus_tag="ACZPFK_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1407133.1"
                     /translation="IGKHVFGGAGSYPFHPAALAYAAVTVSWPDQIFRYPTPFSNVGL
                     FDTSGATLSESFAHILRAGGVPNIPSLDLVLGNYAGPMGATFCIVIAAVAVYLLLKRR
                     ISFELPVAFLVTCGVISFCFPRVSTAARVDLLKYELLSGALIYTAVFLLCESATAPKN
                     RLARVVYGVLTGFLTMMFRYYGAYELGVCFALLLSNAFSGYLDRVCSKITFGRRAKPV
                     "
BASE COUNT          411 a          589 c          624 g          245 t
ORIGIN      
        1 caggatgccg atgtagacca gcgtcaccgg cagcccgcgg aacgtggccg gcaggttgcg
       61 gttctggatc ttgcgctgcc cctcgtcgac cagcagcacg gccagcacat agcccaccga
      121 ggtgccaagg gaaaaggcca gcgtctgggc cagcgtgtag ccgcccagcg tggtcagcag
      181 cagagtgccc agcacgcagc agttgaaagt ggccatgggc agcagctcca catagtcttt
      241 ggcggcgatc ctgccgccca gcagcgccac gcccagcagc accacaaaaa aggcgacgac
      301 gctgcacaaa accagcacca gcggctgaaa ggccgccttg tacgcaaagc tggacccggc
      361 cagcagccgg ttgacgaaat agcccagcag gctgctgaga aactgcacaa aggtcagcag
      421 cgccccaaac ttgagcagct ccaccgagcc gtcgtccacc atctttacca gccgggacac
      481 gccgagagcg cgggtgaaga tggcgttttg ggcggccacc gccaccacgg cgtagacaaa
      541 aaaggcgagc acaggtctca aaacagtcat tttgcgttcg cctccatact cacgacccgt
      601 ttgaagaggt tgcgcatcca gcgccagagg gcggcgagga taccgatgag gataaagccg
      661 ccgcaggtga gctgcgccat gggcaggggg ggccgggcgg ccaccgggtg gccgaacagg
      721 gcgcctgagc ccagcagctc gcgcagcgcg gccaccagga acagggccag cagaaagccg
      781 caggtggtga tcagcccctt gcgcagcgcc gtggagacgc gctctttctg ggggcgctca
      841 taccgcttga tgatgatggg ctccaccacc agcagcggca ggtacagccc caccgcctgg
      901 atgccgacgc cgaacagggc gttggcggcg aaccagaccc cgatatagag cacggcggcg
      961 gtgaccgcat aggtcacgcc gcgaaagcgc agatagttgc cgcgggagag cagggccgcc
     1021 agcacccggg tgggggtgag cagcagcagc accgcaacgc ccagcaccag cgcgttgttc
     1081 aaattggtgg cggcgaccac gatgggcgcc agccccaacc cctgcataaa gacggggttg
     1141 ttgaaaaaga agcggtcccg gtgcatcagc cgatccatgt tctcggcata gtaaagactg
     1201 cgttcctgtt tcatacgggc ttcgccctcc ttccaaaggt gatcttgctg cacacccggt
     1261 cgagatagcc gctgaaagcg ttggagagca gcagcgcaaa gcagaccccc agctcgtagg
     1321 caccgtagta gcggaacatc atggtcagaa agccggtgag cacgccgtac accacccgcg
     1381 ccagccggtt tttgggcgcg gtggcggact cgcaaagcag aaagaccgcc gtgtagatca
     1441 gcgcgccgct gagcagctcg tatttgagca ggtcgacgcg cgccgcggtg gacacgcgcg
     1501 gaaagcagaa gctgatcacg ccgcaggtga ccagaaaggc cacgggcaac tcaaagctga
     1561 tccgccgctt gagcagcaga tagaccgcca ccgcggcgat gacgatgcag aacgtggccc
     1621 ccatggggcc ggcgtaattg cccagcacca ggtccagcga ggggatgttg ggcacgccgc
     1681 ccgcgcgcag gatgtgggcg aacgattccg agagggtggc gccggaggtg tcgaaaagcc
     1741 ccacgttgga gaacggggtg ggatagcgga agatctggtc cggccagctc accgtgacgg
     1801 cggcgtaggc cagggccgcg gggtggaacg ggtagctgcc cgcgccccca aagacatgct
     1861 tgccgatca
//
LOCUS       DBMDYA010000596         1869 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12509_length_1869_cov_1.014706, whole genome shotgun sequence.
ACCESSION   DBMDYA010000596 DBMDYA010000000
VERSION     DBMDYA010000596.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12509_length_1869_cov_1.014706"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="ACZPFK_12315"
     CDS             complement(<1..193)
                     /locus_tag="ACZPFK_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407134.1"
                     /translation="MVEEFTSRLETFPNYKVFETELAGRPFVVETGKMCCLSNGSCLV
                     RYGETAVLCNVTMSEKPREG"
     gene            complement(365..622)
                     /gene="rpsO"
                     /locus_tag="ACZPFK_12320"
     CDS             complement(365..622)
                     /gene="rpsO"
                     /locus_tag="ACZPFK_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1407135.1"
                     /translation="MKSKAEIIAEYRLTETDTGSPEVQVALLTERINHLTQHMKENRH
                     DYHSNRGLLKMVGRRRNLLAYLQKKDVERYRALIARLGLRK"
     gene            complement(874..1779)
                     /gene="ribF"
                     /locus_tag="ACZPFK_12325"
     CDS             complement(874..1779)
                     /gene="ribF"
                     /locus_tag="ACZPFK_12325"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR1407136.1"
                     /translation="MRELEQKPKSRLAVALGYFDGVHLGHRRVLTAAVEAARAAGLQS
                     GVFTFVFGEENPIKGDAILAPEERRRRIGALGADWYYCPSYDDFCGLSPQQFVDDILL
                     ERMNAAAVFCGDNFTFGCGGAGDVTLLRALCSARGVSVTVVPMAREGGEPVSSTRIRD
                     LLLAGEMEGANRLLGEPYALQEPVAHGRKIGRAKLGFPTINQRYQPGMLLPRSGIYIT
                     RALVGGKAYPAATGLGDRPTVGGADITCESFLIGFDGNLYGDAVRLEFFKYIAPTRKF
                     DTLEELKACISDAARQSRAYFAAGR"
BASE COUNT          351 a          596 c          561 g          361 t
ORIGIN      
        1 tgccctcgcg gggcttttcg ctcatcgtga cgttgcacag cacggcggtc tcgccgtagc
       61 gcaccaggca gctgccgttg gacaggcagc acatcttgcc ggtctccacc acaaacgggc
      121 ggccggccag ctcggtctcg aacaccttgt agttggggaa ggtctcgagg cgggaagtga
      181 attcttcgac catggggata gcctcctgaa taatgaaata tatagctgtt tcagaggcag
      241 ccagttttag caataaactc ggcgttttgc acaaagcaaa gcgtccagtt taccgctaaa
      301 cgctgaatgc ccctaaaatc attgaaagga cagccggtaa gaccggctgc cctttctgag
      361 ctcgttattt gcgcaggccc aaccgcgcga tcagggcgcg gtagcgctcg acatcctttt
      421 tctgcaggta ggcgagcagg ttgcggcggc ggccgaccat tttcagcagg ccgcggttgc
      481 tgtggtagtc atgcctgttc tctttcatgt gctgggtgag gtggttgatg cgctcggtga
      541 gcagcgcgac ctgcacctcg ggggaaccgg tatcggtctc ggtgaggcgg tactcggcaa
      601 tgatctcagc tttgctcttc atttttgtgt ctcctttttt atctgcgatt tgccgcggac
      661 taaggcaggg ccggagagcc gcgacctgag cccgggtaag ctaaattatt atagcacaaa
      721 tgaaagggcc tgtaaagaaa tttctttctt tatctccgcg gccctttgcc gtcaaaagcg
      781 gtggaaagcg ggccgcagaa aaagctcgcc ccgccgcggt ttttctttct cccggccagg
      841 cgggcaaacg ctttcagccg gcggcacagc ccctcagcgc ccggccgcaa aataggctct
      901 cgactgccgc gccgcatctg agatgcaggc tttcagctcc tccaaagtgt cgaacttgcg
      961 ggtgggcgcg atgtatttga aaaactcgag ccgcaccgcg tcgccgtaga ggttgccgtc
     1021 aaagccgatc aaaaagctct cgcaggtgat gtccgcgccg cccaccgtgg gccggtcgcc
     1081 cagcccggtg gcggccgggt aggccttgcc gcccaccagc gcccgggtga tgtagatgcc
     1141 gctgcggggc agcagcatgc ccggctggta tctctggttg atggtgggga agcccagctt
     1201 ggcgcggccg atcttgcgcc cgtgggccac cggctcctgc agcgcatagg gctcgcccag
     1261 caggcggttg gccccctcca tctcccctgc cagcagcagg tcccggatgc gggtcgagga
     1321 taccggctcg cccccctccc gcgccatggg caccacggtg accgagacgc cccgcgccga
     1381 gcagagcgcc cgcagcaggg tcacatcccc ggcgccgccg cagccgaacg tgaaattatc
     1441 cccgcagaag acggcggcgg cgttcatgcg ctctaacaga atatcgtcca cgaactgctg
     1501 gggggaaagc ccgcagaaat catcgtagga cgggcagtaa taccagtccg cccccagcgc
     1561 cccgatgcgc ctgcggcgct cctccggcgc caggatggcg tcccccttga tggggttctc
     1621 ctccccgaac acaaaggtga agacgccgct ctgcagcccc gcggcccgcg ccgcctccac
     1681 ggcggcggtg agcacccggc gatggcccag atggacgccg tcgaaatagc ccagcgccac
     1741 agccagccgg ctctttggtt tttgctccag ctctcgcata aaaaagcctc ttttagtaat
     1801 atccgttttt aaaactacaa taagtattat caaggggccg cgcggcggtc agccctcccg
     1861 ctcacaaaa
//
LOCUS       DBMDYA010000597         1867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12520_length_1867_cov_1.233862, whole genome shotgun sequence.
ACCESSION   DBMDYA010000597 DBMDYA010000000
VERSION     DBMDYA010000597.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12520_length_1867_cov_1.233862"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="ACZPFK_12330"
     CDS             <1..478
                     /locus_tag="ACZPFK_12330"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR1407137.1"
                     /translation="VDFLLRRRLVRAGVVADHEQVHCPCEHEEGEQSVWRAALLHTLE
                     VLFYVVVFSFAIHLVMEYFGGELLTGWLSRTRLLQPILAALIGLIPNCAASVLLAQLY
                     LSGTITFASLVAGLSSAAGIGLMVLFRSNRGLRRNLLITAALFAVSAAAGLVLSLF"
     gene            745..>1867
                     /locus_tag="ACZPFK_12335"
     CDS             745..>1867
                     /locus_tag="ACZPFK_12335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1407138.1"
                     /translation="MVDVTIIGCGVIGAATAYELAHYDLKTVVVERENDVATGTTKAN
                     SAIIHAGYDPEPGTLMARLNVEGNRRTKEIAARLDVPCKEIGSLVLAFDEADVKTVEE
                     LYRRGVANGVPGVRVLSAQEVQAMEPNLSKEVKAALLAPSAAIISPWEMCLALAETAV
                     KNGVELRLNSPVTAIEKIEGGYRVTAGDSVIETRAIVNAAGVHADQVNDLAAPHAFTT
                     LPSRGEYYLMDKSQGALVDHVIFQCPNKDGKGVLVAPTVHGNLIVGPNAEDVADGDDV
                     AVTAAGLAFVKEKAAKSVPGINYRDSIRNFSGVRAVTDQPDFVICESPDAKGFINLGG
                     IKSPGLSSAPAIAAEAVKLLAGAGIALREKPSFDDTRSAM"
BASE COUNT          339 a          580 c          610 g          338 t
ORIGIN      
        1 ggtggatttt ctgctgcgcc gccgcctggt gcgggcgggc gtggtggcgg accacgagca
       61 ggtccactgc ccctgcgagc atgaagaggg ggaacagagc gtgtggcggg cggcgctgct
      121 gcacacgctg gaggtgctgt tttacgtggt ggtgttctcc tttgccattc atctggtgat
      181 ggagtatttc gggggcgagc tgctcacggg ctggctctcc cgcacccgcc tgctgcagcc
      241 catcctggcg gcgctgatcg gcctcatccc caactgcgcg gccagtgtgc tgctggccca
      301 gctctatctg tcgggcacca tcacctttgc ctcactggtg gcgggcctct ccagcgccgc
      361 gggcatcggg ctgatggtgc tgttccgctc caaccggggg ctcaggagaa acctgctcat
      421 caccgccgcg ctttttgcgg tctcggccgc ggcggggctg gtgttgtcgc tcttttgaaa
      481 aaccggggca aggcgcagaa aaaaggccgg ccgcgtccgg cggccgccgc cgcatcccat
      541 cctgtggtga ggcgcggtct ttttttcatt ttctttgtaa aaagcctcta gtattttgag
      601 atttgccatg ctataatgaa tacaagctta gagaacagag tcaaagcgaa aaacccgaga
      661 gggccttttt tgctttggct ttttttgttg cctgtcccag aggagcgggg cgggggacga
      721 gagataagga gggtttggac agacatggtt gacgtaacga tcatcggctg cggcgttatc
      781 ggcgcggcca cggcgtacga gctggcccac tacgatctca aaaccgtggt ggtggaacgg
      841 gagaacgacg tggccaccgg caccaccaag gccaacagcg ccatcatcca cgcggggtac
      901 gacccggagc cgggcaccct gatggcgcgg ctcaatgtgg agggcaaccg ccgcaccaaa
      961 gagatcgccg caaggctgga tgtgccctgc aaggagatcg gcagcctggt gctggccttt
     1021 gacgaggccg acgtaaaaac ggtggaagag ctttaccgcc gcggcgttgc caacggtgtg
     1081 ccgggcgtgc gcgtcctctc ggcgcaggag gtgcaggcca tggagccgaa cctctcaaaa
     1141 gaggtgaaag ccgctttgct ggccccctcc gccgccatca tctccccctg ggagatgtgc
     1201 cttgcgctgg ccgagacggc ggtcaaaaac ggggtcgagc tgcggctgaa cagccccgtc
     1261 accgccattg aaaagatcga gggcggttac cgcgtcacgg cgggggacag cgtcattgag
     1321 acccgggcca tcgtcaacgc cgcgggcgta catgccgacc aggtcaacga cctggccgcc
     1381 ccccacgcgt tcaccaccct gcccagccgg ggcgagtact atttgatgga taaaagccag
     1441 ggtgcgctgg tagaccatgt catcttccag tgtcccaaca aggacggcaa gggcgtgctg
     1501 gtggccccca ccgtacacgg caacctgatc gttggcccca acgccgagga cgtggcggac
     1561 ggggacgacg tggcggtgac ggccgcaggc cttgctttcg tcaaagaaaa agcggccaaa
     1621 agcgtgccgg gcatcaacta ccgcgattcc atccgcaatt tctccggcgt gcgcgccgtg
     1681 accgaccagc ctgattttgt catctgcgag agcccggacg caaagggctt catcaaccta
     1741 ggcggcatca aatcccccgg cctctcctcc gcgcccgcca tcgccgccga ggcggtaaag
     1801 ctgctggccg gcgcaggcat cgcgctgcgg gagaagccct ctttcgacga cacccgcagc
     1861 gcgatgc
//
LOCUS       DBMDYA010000598         1865 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12536_length_1865_cov_1.738662, whole genome shotgun sequence.
ACCESSION   DBMDYA010000598 DBMDYA010000000
VERSION     DBMDYA010000598.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12536_length_1865_cov_1.738662"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="ACZPFK_12340"
     CDS             complement(<1..1408)
                     /locus_tag="ACZPFK_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1407139.1"
                     /translation="MFWWILLALVVVFIAVLLVRAARFKPKVNGRAAAAPCEVDAAAA
                     VEHLAQMVRVPTVSSYRLEEFDEEKFEEFRGLLKKFYPKVFETCTYERFDHTEMLFTW
                     KGKSSAAPVVLMAHYDVVPVEESRWRHPPFCGEVFDGELWGRGTLDTKITLMGILEAA
                     ERLIGEGFVPQNDVYFAFAGDEEVNGTGAPAAVQVLKERGVKPAMVVDEGGAVVEGVF
                     PGVKRPMAVVGIGEKGLADLEITAKSEGGHASHPPLHSAVGVLSKAVVACEKHQFKAE
                     ITTPVREMLNTVGPYAPFGLRVVFANLWCFGGLITGVIHKLGGELNAMMRTTMAFTMA
                     RGSKQSNVMPNEATATVNLRLINSTTVEGAAEHLRRAINDPAVEVRVLHGSEASPYAQ
                     VDGEAWRTLAAAVGETWQEAIVTPYLMIACSDSRHFSEICRNVYKFSAMALSGEQRGL
                     IHNDDERVELPTVAKTVEF"
BASE COUNT          325 a          659 c          582 g          299 t
ORIGIN      
        1 aaaattccac cgtcttggcg acggtgggca gctccacccg ctcgtcgtcg ttgtggatca
       61 ggccgcgctg ctcgcccgag agcgccatgg ccgaaaattt gtacacgttc cggcagatct
      121 cggaaaaatg gcgcgaatcc gagcaggcga tcatcagata gggggtgacg atggcctcct
      181 gccaggtctc ccccaccgcc gccgccagcg tgcgccaagc ctcgccgtcc acctgcgcat
      241 agggcgacgc ctccgagccg tgcagcaccc tgacctccac cgccgggtcg ttgatggccc
      301 ggcgcaggtg ctccgctgca ccctccaccg tggtgctgtt gatcagccgc aggttgacgg
      361 tggcagtggc ctcgttgggc atcacgttgg actgcttgga gccgcgggcc atcgtgaacg
      421 ccatggtcgt gcgcatcatg gcgttcagct cgccccccag tttatggatg acgccggtga
      481 tgaggccgcc gaagcaccac aggttggcaa agacgaccct cagaccgaac ggcgcatagg
      541 ggcccacggt gttcagcatc tcccgcaccg gggtggtgat ctcggccttg aactgatgct
      601 tttcacaggc gacgaccgct ttgctgagca cgcccacggc gctgtgcagc ggcgggtggc
      661 tggcgtggcc gccctcgctt ttagcggtga tctccaggtc ggccagcccc ttttcaccga
      721 tgcccaccac ggccatgggc cgcttgacac cgggaaagac gccctcgacc accgcgccgc
      781 cctcgtccac caccatagcg ggcttgacgc cgcgctcctt gagcacctgc accgcggcgg
      841 gcgcgccggt gccgttgact tcctcatccc ccgcaaaggc gaaatagacg tcgttttggg
      901 gcacaaagcc ctcgccgatc agccgctcgg cggcctcaag gatgcccatc agggtgatct
      961 tggtgtccag ggtgccccgg ccccacagct ccccgtcaaa gacctcgccg cagaaaggcg
     1021 ggtgccgcca gcggctctcc tccaccggca ccacgtcgta gtgggccatc agcaccaccg
     1081 gggcggcgct gctcttgcct ttccaggtga agagcatctc agtgtggtca aagcgctcgt
     1141 aagtacaggt ctcgaacacc ttgggataaa actttttcag caggccgcgg aattcctcga
     1201 acttctcctc gtcaaactcc tccagccggt agctggaaac agtgggcacg cgcaccatct
     1261 gcgccaagtg ctccaccgcg gcggcggcgt ccacctcaca gggcgcggcg gccgcgcggc
     1321 cgttgacttt cggcttgaac cgcgccgccc gcaccagcag cacggcgatg aaaaccacca
     1381 ccagggccag caggatccac cagaacatga caacactccc tttcagggcg gcagcgccgc
     1441 ctaaaacaag cctatcttaa aacagtatac cacattcccg cggcggctga aagcgacaaa
     1501 agacgccgcc tgtcctgccg caggccgtga tacggggagg cgcgtggggt ttttcgccgc
     1561 tcccccgccc gccgtccggc gcgccgatgt tggcctcgcc cattgcgccg tagcccagca
     1621 ccaccgccgc agaggtttca gcggagagtt tccggcgaaa gcgctgtgcg ccggagtgca
     1681 aacgtcgtgc gccggggcga aaacaccgtg tgcgggggca aaagcgctgt gcgccggggg
     1741 ccgaaagcac cgtgcgtcgg ggaacgaaag cgccgtgtgc tggggtcgaa aacaccgtgc
     1801 gccgggagaa gccccctccc cgaccttgtg ccgcccgcgg ctgcggcccg gcggcaccgg
     1861 cggcg
//
LOCUS       DBMDYA010000599         1864 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12546_length_1864_cov_1.411231, whole genome shotgun sequence.
ACCESSION   DBMDYA010000599 DBMDYA010000000
VERSION     DBMDYA010000599.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12546_length_1864_cov_1.411231"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="ACZPFK_12345"
     CDS             <1..345
                     /locus_tag="ACZPFK_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1407140.1"
                     /translation="ELSEKMFRTKGATSAVVQKLVQKGLVVQVDGETDSRISRLYPTE
                     KGRQVYKNHLEYDMRYLEYLSKRLHISLEELAQVNRVLRLMNGDFIQRHREGENIVDE
                     WPGKLPPPPQEE"
     gene            complement(654..>1864)
                     /locus_tag="ACZPFK_12350"
     CDS             complement(654..>1864)
                     /locus_tag="ACZPFK_12350"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR1407141.1"
                     /translation="LGISYNNFDLLEDGYGINLRALSMFAAQVYRHDPCTVFLPHLLD
                     ENTYDPVDAALAAKMHKAIAVIQFKLEGQLYRRHPEYGMGGRSLLERVDYQAGTLTLE
                     GRTVPLQDRLFPTVAPADPLALTPEERALIDTLTLSFTHSKTLGRHFRFLFSHGGMYK
                     ICNGNLLYHGCIPMTPDGAFGTVTLDGRRLSGRAYLDAVDRKLHDAFFGAPGSPARED
                     ALDFIWYLWCGPRSPLFGKSKLSTFERYFTDDEALCRETLDPYYTFIERRETCERILA
                     EFGLPPAGSHIVNGHVPVRAGEHPRKGDGLLYIIDGGISKAYHGRTGLGGYTLIFNSH
                     HLALAEHRPFEEIRQNPAASAPKVQVVEPMPRRLRVADTDTGALLRGQIARLEELLAA
                     YRGGLLPERG"
BASE COUNT          316 a          583 c          658 g          307 t
ORIGIN      
        1 gagctgtctg agaagatgtt ccgcaccaag ggcgcgacct ccgcggtggt gcaaaagctg
       61 gtccagaaag ggctggtggt gcaggtggac ggggagaccg attcccgcat cagccgcctg
      121 taccccactg aaaaggggcg gcaggtctac aaaaaccatc tcgaatacga tatgagatat
      181 ctggaatacc tctcaaagcg gctgcatatc agcctggagg agctggccca ggtgaaccgg
      241 gtgctgcggc tgatgaacgg ggattttatc cagcgccaca gagagggcga aaatattgtg
      301 gacgagtggc cgggcaagct cccgcctccc ccgcaagagg agtaaaaaag ccgcgtgcag
      361 ctgcacgcgg ctttttcttt ggcgcggctt cggcagggag cggcggtcgc ccggctctgg
      421 gaagaaaggc ccgcctgctg cagcagcgtc gcttgcaggc cccacatcgc ccgtttccgg
      481 caaaaaggac ccgcctgctg cgcggtgtcg ctggcgggcc cgccggggca gcggcgctcc
      541 gcttgcccgg gggcacccct ttttcgcgtg ggcagctggc gccgcacaaa gttgccctgc
      601 cgggcacccc tcggcgcccg ctgccgtcgg gcgctgtggg agacgcgcct ctttcagccc
      661 cgctcgggca gcagcccgcc ccgataggcg gcgagcagct cttccagccg cgcgatctgc
      721 ccccgcagca gggcgccggt atcggtgtcg gccacccgca ggcggcgggg catcggctcc
      781 acgacctgca ctttcggggc gctggcggcg gggttctggc ggatctcctc aaagggacgg
      841 tgctcggcca gggcgaggtg gtgggagttg aagatcagcg tgtatccgcc caggccggtg
      901 cggccgtgat aggctttcga gatgccgccg tcgatgatgt agagcagccc gtcccccttg
      961 cgcgggtgct cgcccgcgcg caccggcaca tgcccgttga cgatgtggga gcccgcgggc
     1021 gggaggccga actccgccaa aatcctttcg caggtctccc gcctctcgat gaatgtgtag
     1081 taggggtcca gcgtctcccg gcacagggcc tcgtcgtcgg tgaagtagcg ctcaaaggtg
     1141 gagagcttgc tcttgccgaa cagcggcgag cgggggccgc accacagata ccagataaag
     1201 tccagcgcgt cctcccgcgc cgggctgccg ggcgcgccga agaaagcgtc gtgcagcttg
     1261 cggtccacgg cgtccagata ggcgcggccg gacagccgcc tgccgtccag cgtcacggtg
     1321 ccgaaagcgc cgtcgggggt catggggatg cagccgtgat agagcaggtt gccgttgcaa
     1381 attttgtaca tgccgccgtg ggagaacaaa aagcgaaagt gccgccccag ggttttgctg
     1441 tgggtaaaag agagggtcag cgtgtctatg agggcccgct cctccggtgt gagcgccagc
     1501 gggtcggcgg gcgctacagt ggggaacagg cggtcctgca gcggcacggt gcgcccctcc
     1561 agcgtcaggg tgcccgcctg gtaatccacc cgctccagca ggctgcgtcc gcccatgccg
     1621 tattccggat ggcggcggta gagctgcccc tccagcttga actggatgac ggcgatggcc
     1681 ttgtgcattt tggccgccag cgccgcgtcc accgggtcat aggtgttctc gtccagcagg
     1741 tggggaagaa agaccgtgca ggggtcatgg cggtagacct gggccgcgaa catcgagagc
     1801 gcgcgcaggt tgatgccgta gccgtcctcc agcaggtcga aattgttata gctgatgccc
     1861 aggc
//
LOCUS       DBMDYA010000600         1863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12554_length_1863_cov_1.750284, whole genome shotgun sequence.
ACCESSION   DBMDYA010000600 DBMDYA010000000
VERSION     DBMDYA010000600.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12554_length_1863_cov_1.750284"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(305..838)
                     /locus_tag="ACZPFK_12355"
     CDS             complement(305..838)
                     /locus_tag="ACZPFK_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1407142.1"
                     /translation="MRCAHCGAEIGEHELYCPFCGRALPTDELSEAERLLVQKNVAYY
                     GRHFAALRAGKRADWNWAAFLAFPFWAAYRKMVLPALLSFIAFGALFWFRAGWLAPAL
                     MTAFGLLGNRAYYRRTQAMADGLAEEPPERLEQTCLKRGGVTPLLPALLAAALLLLGI
                     WWQVFLQRAALLLYPRG"
     gene            complement(903..>1863)
                     /locus_tag="ACZPFK_12360"
     CDS             complement(903..>1863)
                     /locus_tag="ACZPFK_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR1407143.1"
                     /translation="GIDRVQGEYVAEIKLRHLNREYILKRTDEIAALEKDIAELQGIL
                     DNPSKVKKIIIAELTEVSKKYAQPRRSEILYDAPAAVEEEEEQEMSDYPVTIFYTEEG
                     YFKKITPASLRMSSEQRLKEGDAVRYEIETKNNAWLLFFTNRAQVYKCRVGDFGDTKA
                     SAMGEYLPAKLGMEEGEVPIYVTVTSDYRGHMVFFFQNGKAAKVPLSSYETKQNRRKL
                     VGAYSDKAELAAVRELPEEGELAVQTTNGRLLLVHSTQIPEKATRNTMGVAVITLKKN
                     ARIASVKIAADLELANPHRYRVRTLPAAGALLRPEDVAEQITL"
BASE COUNT          356 a          613 c          549 g          345 t
ORIGIN      
        1 actatagcat actccacgcg caaaagcaag agccacggcc gctaaaagcc ctgtaaaatt
       61 cttttttcgc cgctgtatat ccctcaaaaa agcgggcagc atactaacac atcactcgat
      121 ttacacaaaa cggatactta gagcttcaca caggacgcaa acgccctttc aggactgctg
      181 cggaaaaggt gaaaccggca tccgcagtgt acctgatgat gtcaaaggcc gggcaaatgc
      241 ccggcctttt gctgtttgcc gcaaatgcga aaattaaaga tgaaagcgcc gggccggacc
      301 gcctctagcc gcgcgggtag agcaacagcg cagcccgctg cagaaagacc tgccaccaga
      361 tccccagcag cagcagcgcg gccgccagca gcgccggcag cagcggggtc acgccgcccc
      421 gcttaagaca ggtctgctcc agccgctcgg gcggctcctc ggcaaggccg tccgccatgg
      481 cctgggtgcg ccggtaataa gcccggttgc ccagcaggcc gaatgccgtc atcagggccg
      541 gggccagcca cccggcccga aaccagaaca gcgccccaaa ggcgatgaaa gagagcagcg
      601 cgggcagcac catcttgcga taggccgccc aaaagggaaa ggccaaaaac gccgcccagt
      661 tccaatccgc cctcttgccg gccctcaggg cggcaaagtg ccgcccataa tacgccacat
      721 ttttttgcac cagcagccgc tctgcctccg acagctcatc agtgggcagg gcccggccgc
      781 agaacggaca gtagagctcg tgttcgccga tctcggcgcc acagtgcgca cagcgcatga
      841 cgatccctcc tcttcggatc gaaaggtcaa acaaaaggcc gggggccggc cccgctcaga
      901 gctcagagcg tgatctgctc ggccacgtct tcgggccgca gcagcgcgcc cgcggcgggc
      961 agggtgcgca cccggtagcg gtgcgggttc gccagctcca gatccgccgc gatcttcacg
     1021 ctggcgatgc gggcgttctt cttgagggtg atcaccgcca cgcccatggt gttgcgggtc
     1081 gccttttcgg gtatctgggt ggagtggacc agcagcaggc ggccgttcgt ggtctgcacc
     1141 gccagctcgc cctcctccgg cagctcgcgc acggcggcca gctcggcctt gtcgctgtag
     1201 gcccctacca gcttgcgccg gttctgcttg gtctcatagc tggagagggg caccttggcg
     1261 gccttgccgt tctggaagaa aaagaccatg tgcccccggt aatcgctagt caccgtgaca
     1321 tagatgggga cctccccctc ctccatgccc agcttggcgg gcagatactc gcccatggcg
     1381 ctggccttgg tatcgccaaa atcacccaca cggcacttgt acacctgggc gcggttggtg
     1441 aaaaagagca gccaggcgtt gtttttggtc tcgatctcat accgcaccgc gtcgccctct
     1501 ttcaggcgtt gctcgctgct catgcgcagc gaggcgggcg tgatcttttt aaagtagccc
     1561 tcctcggtgt aaaagatggt caccgggtag tcggacatct cctgttcttc ctcctcctcc
     1621 accgcggcgg gcgcgtcgta gaggatctcg ctgcgccggg gctgggcgta ctttttcgac
     1681 acctcagtca gctcggcgat gatgatcttt ttcaccttgg aggggttatc caggatgccc
     1741 tggagctctg cgatatcctt ttccagggcc gcgatctcgt cggtgcgctt gaggatatat
     1801 tcccggttga ggtggcgcag cttgatctcc gccacatact cgccctgcac ccggtcgatg
     1861 ccg
//
LOCUS       DBMDYA010000601         1863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12555_length_1863_cov_1.528377, whole genome shotgun sequence.
ACCESSION   DBMDYA010000601 DBMDYA010000000
VERSION     DBMDYA010000601.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_1863_cov_1.528377"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="ACZPFK_12365"
     CDS             complement(<1..339)
                     /locus_tag="ACZPFK_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407144.1"
                     /translation="MAIGLTDDGIIIGVNIDVNRIQQAIAKMKSLMEDFASGTVKSVQ
                     KADKELEKARKNAEKWKIEPTTEGIEAAAKELDRLNAVIVNQQAELANCEREHERVAE
                     RYGETSSQALK"
     gene            complement(317..877)
                     /locus_tag="ACZPFK_12370"
     CDS             complement(317..877)
                     /locus_tag="ACZPFK_12370"
                     /inference="COORDINATES: protein motif:HMM:NF018553.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HIR1407145.1"
                     /translation="MLDLPREILGQAVETDWLWWMRRVGYVLDSSLLWAEKADILLLN
                     VYRAIPDDPKQATGRLQGVLDFYLCGDRGEPDEEPPREKLLDWRKDRLRIWGDFKVYA
                     GIDLNTARMHWWEFMAIFNSLPPDSQIKHAIGLRALDLSELEDPKQREYYARAKRAIA
                     LEPVDYEAEYDAAMERRTCLGNWTNR"
     gene            complement(762..1316)
                     /locus_tag="ACZPFK_12375"
     CDS             complement(762..1316)
                     /locus_tag="ACZPFK_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407146.1"
                     /translation="MGFTFAEKHMIEICGKRYPCDIGDCRMLEGVARDFPRILGAAQE
                     LLQIDAQLKATVRDKAPARELGEELIERNNALLAACKTFVEGTLGEAEYREIFAGRPA
                     NTEEHIELCAYIYSDIMGGRREIVNEYLDPAVREEGQTTNGAEPDAGSPPGDIGTGGG
                     DGLAVVDAPRGLCARLLTALGRKG"
     gene            complement(1332..1838)
                     /locus_tag="ACZPFK_12380"
     CDS             complement(1332..1838)
                     /locus_tag="ACZPFK_12380"
                     /inference="COORDINATES: protein motif:HMM:NF047353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098026 - virus tail, tube [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR1407147.1"
                     /translation="MPGTYVKPKAYNRRWWIDIGTDNTAQWAEIARGITSRGNSISES
                     KDDYYDMAGRGVAESEVSGITVQRSFSGFRVMGDEAQDNIIERLYDLEHRAVKFLECY
                     DNPAAAGAFKGRQGMATLSITDDGSGDANKRENIAFALNIEGTPAKGTVAIAADGTPT
                     FTADAAEQ"
BASE COUNT          307 a          640 c          478 g          438 t
ORIGIN      
        1 cttcagcgcc tggctgctgg tctcgccgta ccgctccgcc acacgctcat gctcccgctc
       61 gcagttggcg agctctgcct gctggttcac gatgacggcg ttcagccggt ccagttcttt
      121 cgccgctgcc tcgatgcctt ccgtagtggg ctcaatcttc catttctcgg cgtttttacg
      181 ggccttttct agttctttat ctgccttttg cacggatttt acggtgccgg aggcaaagtc
      241 ctccatgagg cttttcatct tggctattgc ctgctggatg cggttgacgt ctatattcac
      301 accaatgatg attccatcat ctgttagtcc aattgccaag acacgtcctc ctctccattg
      361 ccgcatcgta ctcggcctcg taatccacag gctccagcgc tattgcccgc ttcgcgcggg
      421 catagtactc gcgctgcttc gggtcctcaa gctccgaaag atccagcgcc cgcagcccta
      481 tggcatgctt gatctggcta tcgggcggca agctgttgaa gatcgccata aactcccacc
      541 agtgcatccg cgcggtattg agatcaatcc cggcgtatac cttaaaatcc ccccatatcc
      601 gcagccggtc ctttcgccag tccagcagct tttcccgcgg cggttcttcg tccggctccc
      661 cacggtcgcc gcacaaataa aaatcgagca ccccttgcag gcgcccggtt gcttgttttg
      721 ggtcgtccgg gatggcccgg tacacgttga gcagcaggat atcagccttt tcggcccaga
      781 gcagtgagga gtcgagcaca tagcccacgc ggcgcatcca ccacagccag tccgtctcca
      841 ccgcctgtcc caatatctcc cgggggagat ccagcatcag gttctgctcc atttgtcgtc
      901 tgcccctcct cccggaccgc agggtcgaga tactcgttga cgatctcccg gcggccgccc
      961 atgatatccg agtagatata cgcgcacagc tcgatgtgct cctccgtgtt ggccggacgg
     1021 ccggcaaaga tctcccggta ctctgcctcg cccaacgtgc cctctacaaa tgtcttgcag
     1081 gccgccagca gcgcattgtt gcgctcaatc agctcttcgc ccagctcccg ggcaggggct
     1141 ttgtcccgca cagtggcctt gagctgcgcg tcaatctgta gcagctcctg ggcggccccg
     1201 aggatgcgcg gaaaatcccg cgccacaccc tccaacatcc ggcagtcccc gatgtcgcag
     1261 ggatatcgct tgccgcagat ttcgatcata tgcttttcag caaaagtaaa tcccacgccg
     1321 ccgcctcctt tttactgctc ggccgcgtcc gccgtaaaag tgggggtgcc gtcggccgcg
     1381 atggcgacgg tgccttttgc cggtgtgccc tcaatgttga gggcaaaggc gatattttcg
     1441 cgcttgttgg cgtcgccgct gccgtcgtcc gtgatggaga gggtcgccat gccctggcgg
     1501 cccttgaacg cccccgcggc cgcggggtta tcatagcact ccaaaaactt gaccgcccga
     1561 tgctccaagt catacaggcg ctcgatgatg ttgtcctgcg cttcgtctcc catcacccgg
     1621 aaccccgaaa agctccgctg cactgtgatg ccggagacct ccgactccgc cacgccccgg
     1681 cccgccatgt cataatagtc gtccttgctc tcgctgatgc tgttcccccg cgaggtgatc
     1741 cccctcgcga tctccgccca ctgggccgta ttgtccgtgc cgatgtcgat ccaccaccgg
     1801 cgattatatg ctttcggctt tacgtaagtt ccaggcatcc tcttaaccct ctttctcaat
     1861 gat
//
LOCUS       DBMDYA010000602         1862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12563_length_1862_cov_1.392391, whole genome shotgun sequence.
ACCESSION   DBMDYA010000602 DBMDYA010000000
VERSION     DBMDYA010000602.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12563_length_1862_cov_1.392391"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="ACZPFK_12385"
     CDS             complement(<1..1169)
                     /locus_tag="ACZPFK_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HIR1407148.1"
                     /translation="MSQTQAQTAASPRLDYPKFTKEMRLTHKILIPQMLPIHFGLIEK
                     VLRQEGYDVEVLKNDGPAVVQTGLKYVHNDTCYPALLVIGQMIDALDSGRYDLDHTAL
                     MITQTGGGCRASNYIHLLRKALKKAGYGQVPVVSLNFSGLEKDSGFQLTLPLIEKVLG
                     AVFYGDMLMLLSNQLRPYEDRKGDADRLVNYWVAMLSDQFATGHGCLKREMARNFDAI
                     AKTFADLHVTRTPKVKVGVVGEIYIKYSPLGNNQLERFLAGEDCEVNVPGLLGFILYC
                     VANGAETIDLYGGSKAVKSVYGLVMEYFAKRERMMIASISKYPGLTPPPLFSATMKCA
                     DGIIGYGNKMGEGWLLTAEMMELEQHGVDNIICAQPFGCLPNHICGKGMMNRIRSV"
     gene            complement(1285..>1862)
                     /locus_tag="ACZPFK_12390"
     CDS             complement(1285..>1862)
                     /locus_tag="ACZPFK_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1407149.1"
                     /translation="GIHRPRDFMAKMPVILANYFDGISAGEVRRAAKAAYAEYEAHMQ
                     RIRAEGARVIAAARAEGKPIVVLSGRPYHLDPEVNHGIDKLICSQGAALVSEDSVAHL
                     AGRQQVGVLNQWTYHSRLYAAAAYVTTQPDMELVQLVSFGCGVDAITTDEVKAILQEK
                     GKIYTQIKIDEITNLGAVNIRLRSLFAAIGR"
BASE COUNT          299 a          578 c          629 g          356 t
ORIGIN      
        1 accgagcgga tgcggttcat catgcccttg ccgcagatgt ggttgggcag gcagccaaag
       61 ggctgggcgc agatgatgtt gtccaccccg tgctgctcca gctccatcat ctcggcggtg
      121 agcagccagc cctcgcccat cttgttgccg tagccgatga tgccgtcggc gcatttcatg
      181 gtggccgaga acagcggcgg cggcgtgagg ccgggatatt tggagatgga cgcgatcatc
      241 atccgctcgc gcttggcaaa gtactccatc accaggccgt agaccgactt gacggccttg
      301 ctgccgccgt agaggtcgat ggtctcggct ccgtttgcca cacagtagag gataaagccc
      361 agcagccccg gcacgttgac ctcacagtcc tcgccggcga ggaagcgctc gagctggttg
      421 ttgcccagcg gggagtactt gatatagatc tcgcccacca cgcccacctt caccttgggg
      481 gtgcgggtga cgtgcaggtc ggcgaaagtc ttggcgatgg cgtcgaaatt gcgcgccatc
      541 tcccgcttta agcagccgtg cccggtggcg aactggtcgc tgagcatggc cacccagtag
      601 ttgaccaggc ggtcggcatc gcccttgcgg tcctcatagg ggcgcagctg gttggacagc
      661 agcatcagca tgtcgccgta gaacaccgcg cccagcacct tttcgatcag cggcagggtg
      721 agctgaaagc cgctgtcctt ttccaggccg gaaaagttca gcgagaccac cggcacctgc
      781 ccgtagccgg cctttttcag ggccttgcgc agcaggtgga tgtagttgga ggcccggcag
      841 ccgccgccgg tctgggtgat catcagggcg gtgtggtcca ggtcgtacct gccgctgtcc
      901 agcgcgtcga tcatctggcc gatgaccagc agcgccgggt agcaggtgtc gttgtggacg
      961 tatttgaggc ccgtctgcac cacggcgggg ccgtcgtttt tgagcacctc gacgtcatac
     1021 ccctcctgcc gcaacacctt ttcgatgagg ccgaaatgga tgggcagcat ctgggggatc
     1081 aaaattttgt gggtgagccg catctccttg gtgaacttgg gatagtccaa acggggggag
     1141 gctgcggtct gagcttgggt ctgagacatg ggtcggaact cctttccgtg agcgcagaga
     1201 gcggcgctct ctgcgcgggc ggcgcgggca gggaggcggc agggggccgc cgccctttgc
     1261 ccgggcgcgc cgggtggaac aaacttaccg cccgatggcg gcgaacaggc tgcgcagccg
     1321 gatattgacc gcgcccaggt tggtgatctc gtcgatcttg atctgggtgt agattttgcc
     1381 cttttcctgc aggatggcct tgacctcgtc ggtggtgatg gcgtccaccc cgcagccgaa
     1441 gctgaccagc tgcaccagct ccatatccgg ctgggttgtg acataggcgg ccgcggcgta
     1501 caggcggctg tggtaggtcc actggttgag cacccccacc tgctggcggc cggccagatg
     1561 ggcgacgctg tcctcggaca ccagggccgc gccctggctg cagatcagct tgtcgatgcc
     1621 gtgattgacc tccgggtcca ggtggtaggg gcggccggag agcaccacga tgggcttccc
     1681 ctccgcccgc gccgccgcga tgacccgcgc gccctcggcc cggatgcgct gcatgtgggc
     1741 ctcgtactcg gcataggccg ccttggcggc ccgccgcacc tcgcccgcgg agatgccgtc
     1801 aaaatagttt gccaggatca ccggcatctt cgccatgaaa tcgcgggggc ggtggatgcc
     1861 ga
//
LOCUS       DBMDYA010000603         1859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12581_length_1859_cov_1.610353, whole genome shotgun sequence.
ACCESSION   DBMDYA010000603 DBMDYA010000000
VERSION     DBMDYA010000603.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_1859_cov_1.610353"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="atpA"
                     /locus_tag="ACZPFK_12395"
     CDS             complement(<1..938)
                     /gene="atpA"
                     /locus_tag="ACZPFK_12395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1407150.1"
                     /translation="MQLNASDITKVIKSQIKNYENRVEQSETCAVMLVGDGIARAYGL
                     QNCMANELVEFESGAYGMALNLEEDAVSIVVLGSDEGIREGDVVRRTGRVVSVPVGEG
                     FLGRVVDALGAPIDGKGPIEAVETRPIERPAPGIIERKSVSVPLETGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIATDTILNQKGKGVLCVYVAIGQKRSTVAQLAETLAAAGAM
                     DYTVIVAATASELAPMQYIAPYSGCTMAEYFMDQGRDALIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAARLSPEAGGGSL"
     gene            complement(1062..1598)
                     /gene="atpH"
                     /locus_tag="ACZPFK_12400"
     CDS             complement(1062..1598)
                     /gene="atpH"
                     /locus_tag="ACZPFK_12400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1407151.1"
                     /translation="MTGAAVHYGDALFELAGAEGCDGRILEELDAVAALLRAEPAYLR
                     VLDAPTLRREERCALLDEAFGGRVHPYLLNTLKLMAERGLAKGFFDAAAQYRKQYNGA
                     HGILEARAVTAVPLSEGLRDRLAAALARATGKEIRLTCAVDGALLGGVRLELEGKELD
                     GTVRHRLDALRRALLRAD"
     gene            complement(1595..>1859)
                     /locus_tag="ACZPFK_12405"
     CDS             complement(1595..>1859)
                     /locus_tag="ACZPFK_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012645.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B family protein"
                     /protein_id="HIR1407152.1"
                     /translation="EIVKTATAAAQRRGEEIVKDAKDSAEALRRRTEEELRREKRRAE
                     HEIKDEVSGIALEIAGRVVEKEIDPARHEKLIEEFISGLGDAS"
BASE COUNT          251 a          691 c          593 g          324 t
ORIGIN      
        1 aggctgccgc cccccgcctc cggcgagagc cgggccgccc gctccaacag gcggctgtgc
       61 aggtaaaaga cgtcgccggg gtacgcctcg cgtcccggcg gccgccggat gagcagcgac
      121 agggcccggt aggccaccgc gtgcttggag agatcgtcat agatgatgag ggcgtccctg
      181 ccctgatcca tgaaatactc ggccatcgtg cagccggaat agggcgcgat atactgcatg
      241 ggcgccagct cgctggcggt ggcggccacg atgacggtgt aatccatcgc gcctgcggcg
      301 gccagcgtct cggccagctg ggccacggtg ctgcgcttct ggccgatggc cacatagacg
      361 cagagcaccc ccttgccctt ttggttgagg atggtgtcgg tggcgatggc ggtcttgccg
      421 gtctgccggt cgccgataat cagctcgcgc tggccgcggc cgatggggat catgctgtcg
      481 atggccttga tgccggtctc cagcggcacg ctcaccgact tgcgctcgat gatgcccggc
      541 gcgggccgct cgatggggcg cgtctccacc gcctcgatgg gtcccttgcc gtcgatgggc
      601 gcgcccagcg cgtccaccac ccggccgaga aagccctcgc ccaccggcac cgagaccacc
      661 cggccggtgc ggcgcaccac gtcgccctcg cggatgccct cgtcagagcc cagcaccacg
      721 atggagaccg cgtcctcctc caggttgagg gccatgccgt aggcgccgct ctcaaactcc
      781 accagctcgt tggccatgca attctgcagg ccgtaggcgc gggcaatgcc gtcgcccacc
      841 agcatgacgg cgcaggtctc gctctgctcg acccgatttt cataattttt gatctggctc
      901 ttgatcacct tggtgatgtc gcttgcattc agctgcatct tgtgtggcca tcctttcccc
      961 gcgggccctg cgccgccggc cccgtgccgg cgtacgcccc gcggccgcct ccctcaaaac
     1021 acattttttg agagaggccg cccccgcgtc ccgtttcccg ttcaatcagc gcgcagcagc
     1081 gctctgcgca gcgcgtccag ccggtggcgc accgtgccgt ccagctcctt gccctccagc
     1141 tccagccgca cgccgcccag cagggcgccg tccacagcgc aggtcagccg gatctctttg
     1201 ccggtggccc gcgccagggc cgcggccaga cgatcccgca gcccctcgct cagcggcacg
     1261 gcggtgacgg cccgcgcctc aagaatgccg tgggccccgt tgtattgctt gcggtattgg
     1321 gccgcggcgt caaagaagcc ctttgccagc ccgcgctccg ccatcagctt gagggtattc
     1381 agcagatagg ggtgcacccg gccgccaaag gcctcgtcca gcagggcgca gcgctcctcc
     1441 cggcgcaggg tgggcgcgtc cagcacccgc aggtacgccg gctccgcccg cagcagcgcc
     1501 gcgacggcgt ccagctcctc caggatgcgg ccgtcgcagc cctccgcccc ggccagctcg
     1561 aacagcgcgt ccccgtagtg cacggcggcc ccggtcacga ggcgtcaccc agaccggaga
     1621 tgaattcttc gatcagcttt tcgtggcggg cggggtcgat ctccttttcc accacccggc
     1681 ccgcaatctc cagcgcaatg ccggagacct cgtccttgat ttcgtgttcg gccctgcgct
     1741 tttcacggcg cagctcctcc tcggtgcggc ggcgcagcgc ctcggcgctg tcctttgcgt
     1801 cttttacgat ctcctcgccg cggcgctggg ctgcggcggt ggcggttttg acgatctcg
//
LOCUS       DBMDYA010000604         1855 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12615_length_1855_cov_1.761117, whole genome shotgun sequence.
ACCESSION   DBMDYA010000604 DBMDYA010000000
VERSION     DBMDYA010000604.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12615_length_1855_cov_1.761117"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="ACZPFK_12410"
     CDS             <1..571
                     /locus_tag="ACZPFK_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407153.1"
                     /translation="LASVAAATVVALLLGVLLSRAPRIAGFVMPVISIFQTIPGIVFI
                     GILYLYLGMVPVTAFTALAIYAIFPILKNTYAGLLGVEEKYKEAARGCGMNTRQILFK
                     VELPLAMPTIFAGIRMSTVYTVSWAVLTAMIGLGGLGEFVYRGVSTNNSTLTVIGAVP
                     AALMAIGLGALIDWIQKKVTPRGLQEVKN"
     gene            571..1164
                     /locus_tag="ACZPFK_12415"
     CDS             571..1164
                     /locus_tag="ACZPFK_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407154.1"
                     /translation="MDWSRVWEHFTIVAIASVFTILLGLLLGFTAHLNKRAGKVILAV
                     VDVLQTIPALALLGIIMVFFGGSRTTVIVGLVLYSLLPVVRNTCTGLESVSPAVKEAA
                     LGMGMSKTYRLFKVEFPIAFPTIFTGIRIAVVTSISVAVFGTFVGGGGLGQVIYRGIY
                     VQDMTRILFGTVSLMVMAVVLDFIMSLVEKRLYRHQM"
     gene            1278..>1855
                     /locus_tag="ACZPFK_12420"
     CDS             1278..>1855
                     /locus_tag="ACZPFK_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015995.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1407155.1"
                     /translation="MKRLKKLSALLLGVLMAATLLSGCGGKKNTVVILDGEFAEPEIV
                     AEMAKLLIEDKTDLKAEIKDAMTPVFGFNEMNEGKIDLMLSYDGSLLVTFLKHDDSEV
                     PEGMSTYDYAQQVAAEECKVHLMEKLGANNTYSIAVRRETAEKYNLKTISDLVPVAGE
                     LVFGAEHKFFDEEGLIRYKPMCKEYGLEFKAG"
BASE COUNT          344 a          563 c          596 g          352 t
ORIGIN      
        1 gctcgcctcg gtggccgcgg ccacggtggt ggcgctgctg ctgggggtgc tgctctcgcg
       61 ggcgccccgc atcgccggct ttgtcatgcc ggtcatctcg atctttcaaa ccatccccgg
      121 catcgtcttt atcggcatcc tctacctgta cctgggcatg gtgccggtca ccgctttcac
      181 ggcgctggcc atctacgcca tcttccccat cctcaaaaac acctacgcgg ggctgctggg
      241 cgtggaggaa aaatacaagg aggcggcgcg gggctgcggc atgaacacgc gccagatcct
      301 cttcaaggtg gagctgccgc tggccatgcc caccatcttt gcgggcatcc gcatgtccac
      361 cgtctacacg gtcagctggg cggtgctcac cgctatgatc ggcctgggcg gcctgggcga
      421 gttcgtctac cggggcgtgt ccaccaacaa cagcaccctc accgtgatcg gcgcggtgcc
      481 cgccgcgctg atggccatcg gcctgggcgc cctgatcgac tggatacaga aaaaggtcac
      541 tccgcgcggc ctgcaggagg tgaagaactg atggattgga gcagagtttg ggagcacttt
      601 accatcgtcg ccatcgccag cgtcttcacg attttgctgg ggctgctgct gggcttcacc
      661 gcccacctga acaagagggc gggcaaggtc atcctggccg tggtggacgt gctgcagacc
      721 atcccggccc tggcgctgct gggcatcatc atggtgttct tcggcggcag ccgcaccacc
      781 gtcatcgtcg gcctggtgct gtactcgctg ctgccggtgg tgcgcaacac ctgcaccggg
      841 ctggagagcg tgagcccggc cgtcaaggag gcggcgctgg gcatgggcat gagcaagacc
      901 taccgcctgt tcaaggtgga gtttcccatc gcttttccca ccatcttcac cggcatccgc
      961 atcgcggtgg tcacctccat cagcgtggcg gtgttcggca cctttgtggg cggcggcggc
     1021 ctgggccagg tcatctaccg cggcatctat gtgcaggata tgacccgcat cctcttcggc
     1081 accgtgtcgc tgatggtgat ggccgtggtg ctggacttta tcatgtcact ggtggaaaag
     1141 aggctttacc ggcaccagat gtgagggcgg cgcgcctttt ggcgggagga gcggcagcgg
     1201 ccgccacgcc tctgccgcca cgtcttttaa atagaacgtc aatctgattt ttgaaacgga
     1261 aaaaaggagg acaccccatg aagagattga aaaaattatc cgccctgctg ctgggcgtgc
     1321 tgatggcggc cacgctgctg tccggctgcg gcggcaaaaa gaacaccgtg gtcatcctgg
     1381 acggcgagtt cgccgagccg gagatcgtgg ccgagatggc aaagctgctc atcgaggaca
     1441 agaccgacct gaaagcggag atcaaggacg ccatgacccc ggtgttcggc ttcaacgaga
     1501 tgaacgaggg caagatcgac ctgatgctca gctatgacgg cagcctgctg gtcacctttc
     1561 tcaagcacga cgactcggag gtgcccgagg gcatgagcac ctacgactac gcccagcagg
     1621 tggcggcgga ggagtgcaag gtccatctga tggaaaagct gggcgcgaac aacacctatt
     1681 ccatcgccgt gcgccgtgag acggcggaga agtacaacct caagaccatc agcgacctgg
     1741 tgccggtggc gggcgagctg gtgttcggcg ccgagcacaa gttcttcgac gaggagggcc
     1801 tgatccgcta taagcccatg tgtaaggaat acggcttaga gttcaaggcc ggcaa
//
LOCUS       DBMDYA010000605         1854 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12624_length_1854_cov_1.299487, whole genome shotgun sequence.
ACCESSION   DBMDYA010000605 DBMDYA010000000
VERSION     DBMDYA010000605.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_1854_cov_1.299487"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="ACZPFK_12425"
     CDS             complement(<1..1254)
                     /locus_tag="ACZPFK_12425"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1407156.1"
                     /translation="MGKSIAQKIIAAHLVDGEMAPGKEVGLRIDQTLTQDATGTMAYL
                     EFEAMGIPRVKTELSVAYIDHNTLQNGFENADDHNFIRSIAKRIGVRYSRPGNGICHQ
                     VHLERFAVPGKTLIGSDSHTPTAGGIGSLAIGAGGLDVAVAMGGGAYYIPYPEIIKVN
                     LTGRLRPYVSAKDVILELLRILSVKGGVGKVVEYGGEGVKTLSVPERATITNMGAELG
                     ATSSIFPSDEATRTFLAAQGREADWQPLDADPDAEYSRVIEIDLSALEPLAACPHSPD
                     NVKPVRSLGLIKVDQVCIGSCTNSSLADLLTVAAMLKGRTVAPDVELSISPGSKQVLT
                     MLAENGALADLIDAGARILESACGPCIGMGFSPNSKGVSLRTFNRNFEGRSGTQDASV
                     YLVSPETAAASALSGYFTAPTPLPPM"
     gene            1453..>1854
                     /locus_tag="ACZPFK_12430"
     CDS             1453..>1854
                     /locus_tag="ACZPFK_12430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR1407157.1"
                     /translation="MLLENLQSAVQEEEKKKGGDDAPDGAEIDRAQLEKDYILETGSV
                     LKTRGRHAIHCLTVIGQIEGHVVSPPTQKTTKYEHVIPQLVAVQEDPEIEGLLIILNT
                     VGGDVEAGLALAELIAGVAKPTASLVLGGGHS"
BASE COUNT          305 a          551 c          674 g          324 t
ORIGIN      
        1 catggggggg aggggggtgg gggcggtgaa atagcccgac agggcgctgg cggccgccgt
       61 ctcagggctg accaggtaga cgctggcgtc ctgggtgccg ctgcggccct caaaattgcg
      121 gttgaacgtg cgcagcgaga cccccttgct gttgggggag aagcccatgc cgatgcaggg
      181 gccgcaggcg gattccaaaa tgcgggcgcc cgcgtcgatg agatcggcca gcgcgccgtt
      241 ttccgccagc atggtcagca cctgcttgga gcccgggctg atcgacagct ccacgtccgg
      301 ggcgaccgtg cgccccttga gcatcgccgc cacggtcaaa aggtcggcca ggctggagtt
      361 ggtgcagctg ccgatgcaga cctggtccac tttgatgagg cccagcgagc gcaccggctt
      421 gacgttgtcg gggctgtgcg ggcaggcggc cagcggctcg agcgccgaca ggtcgatctc
      481 gatgacgcgg ctgtactcgg cgtccgggtc ggcgtccagc ggctgccaat ccgcctcgcg
      541 gccctgcgcc gccaaaaagg tgcgggtggc ctcatcggag gggaagatgc tgctggtggc
      601 gcccagctcg gcgcccatgt tggtgatggt ggcgcgctcg ggcaccgaca gggtcttgac
      661 gccctcgccg ccgtactcga ccaccttgcc cacgccgccc ttgaccgaca ggatgcgcag
      721 cagctcgagg atgacgtcct tggcgcttac ataggggcgc agccggccgg tcaggttgac
      781 cttgatgatc tcggggtagg ggatgtagta agcgccgccg cccatggcca ccgccacgtc
      841 caggccgccg gccccgatgg cgaggctgcc gatgccgccc gcggtggggg tgtggctgtc
      901 gctgccgatc agggtcttgc cgggcacggc gaaccgctcc agatggacct gatggcagat
      961 gccgttgccg gggcgggaat agcgcacccc gatgcgtttt gcgatggagc ggataaagtt
     1021 gtggtcatcc gcgttttcaa agccgttctg cagcgtgttg tggtcgatgt aggccaccga
     1081 gagctcggtc ttgacccggg ggatgcccat ggcctcaaat tcaaggtaag ccatggtgcc
     1141 ggtggcgtcc tgggtcaggg tctggtcgat ccgcaggccg acctctttgc ccggggccat
     1201 ctcgccgtcc accaggtggg cggcgatgat cttttgcgcg atgctttttc ccattgtgtt
     1261 cccctcgctt gtcccggcgt tgtgcggccg gcgccctggg cgccggcgga tggggctgcg
     1321 cgccgccgga tagatttata ccacttaata ctatctattt tcacagcgct tgtcaatgtt
     1381 ttaacaggtt gcgggcgggg gaataattgc ccgcaaaccg ttgaaactaa aggggtagaa
     1441 aggcggtgcg gcatgttgct ggaaaatctt caaagcgctg tgcaggaaga ggagaagaaa
     1501 aagggcgggg acgacgcccc ggacggggcg gagatcgacc gcgcccagct ggaaaaggac
     1561 tatatcctgg agaccgggtc ggtgctcaag acgcggggac ggcacgccat ccattgcctg
     1621 acggtgatcg ggcagatcga ggggcatgtg gtctcgcccc cgacccagaa gaccacaaaa
     1681 tacgagcacg tcatcccgca gctggtggcg gtacaggagg acccggagat cgagggcctg
     1741 ctgatcatcc tgaacacggt gggcggggac gtggaggccg ggctggccct ggcggagctg
     1801 atcgccgggg tggcaaagcc caccgcctcg ctggtgctgg ggggcgggca ttcc
//
LOCUS       DBMDYA010000606         1848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12670_length_1848_cov_1.084717, whole genome shotgun sequence.
ACCESSION   DBMDYA010000606 DBMDYA010000000
VERSION     DBMDYA010000606.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12670_length_1848_cov_1.084717"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(234..1079)
                     /locus_tag="ACZPFK_12435"
     CDS             complement(234..1079)
                     /locus_tag="ACZPFK_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1407158.1"
                     /translation="MEPHHLTPEDLDRYLGRLRAEERSAATLEKYTRDLRKFYLFLPA
                     EKLVDKEAVLAYKQALAGAYRPASVNSMLVALNTLLAYLGWGECRVRLFRLQRQTFSS
                     GERELRKEEYLRLLAAARRGGDERLCLVLQTICATGIRVSELRFITAEAVRGGSARVD
                     CKGKLRVIWLPRQLRALLAAYCRRMGVRSGSVFRTRGGRPLDRSNLWAQMKKLCAAAR
                     VAPGKVFPHNLRHLFARTYYRAQKDIVRLADLLGHSSLETTRIYTLSSGAEQQRQIDR
                     LGLVV"
BASE COUNT          290 a          604 c          601 g          353 t
ORIGIN      
        1 gctttttcaa tttgattcaa aaatattttg ccggtttatg tattgcattt ttgggctgga
       61 acggctacaa ttaccattaa tgtggggatt ttgtgaaaag cctgcatgga taccacataa
      121 tgcagattat gtggttttgc tttttgccgt cgcaccgccg cttttatgcc ttttccggcc
      181 gtctcttgcc ggggaaagac aaaggcggct ttttcttttg ggcacagaaa aaatcagacg
      241 accagcccca gccggtcgat ctgccgctgc tgctcggcgc cgctgctcag ggtgtagatg
      301 cgggtggtct ccaagctgct gtggcccagc aggtcggcca ggcgcacgat gtccttttga
      361 gcccggtaat aggtgcgggc gaacagatgc cgcagattgt gcggaaatac cttgcccggg
      421 gcgacccgcg ccgcggcgca gagctttttc atctgggccc agagattgct gcggtccagc
      481 ggccggccgc cccgggtgcg gaacacactg ccgctgcgca cgcccatgcg gcggcagtag
      541 gcggccagca gcgcccgcag ctgccggggc agccagatca cccgcaattt gcccttgcag
      601 tccacccggg cactgccgcc gcgcaccgcc tcggcggtga tgaagcgcag ttcgctgacc
      661 cgaatgccgg tggcgcagat ggtctgcagc accaggcaga ggcgctcgtc cccgcccctg
      721 cgcgcggcgg cgagcaggcg gaggtattcc tccttacgca gctcccgctc accgctgctg
      781 aaggtctgcc gctgcagccg gaacagccgc acccggcatt ccccccagcc gagataggcg
      841 agcagggtgt tcagcgccac cagcatcgag ttgacgctgg cgggccgata agctccggcc
      901 agcgcctgct tgtaggccag cacggcctct ttgtccacca gtttttccgc cggcagaaaa
      961 aggtagaatt tccgcaggtc gcgggtgtac ttttccagcg tggcggcgct gcgctcctcc
     1021 gcgcgcagac ggccgagata gcggtcgagg tcttccggcg tcaggtgatg cggttccata
     1081 gggggatgct cctttcgttt cgcccgcgtg tggcggggat cagtccggct ttattctgtc
     1141 cgcgcaaggc gggcttgata cctttttatc tgccggaaga aaagagcgtc cccgatcccg
     1201 cggacggcag ggcgctgccg ccgggccgcg gcaaagcaca agacgcgccg cccctgcctc
     1261 cccccggccg ggagaggcgg gcgcggcggg gcgcaaaggg gagcaataag accgcggcaa
     1321 ccgccgtgac aagagttgga aacgcatcgc cgccgcagtg attccccctc agacgaccgc
     1381 ggccgccgtt tcccctctcg cgggagccgt gcggcagccc cccgcgggcc tgcgcgggga
     1441 cccaaatacc cgccgggccg ggggaacctc ggggcgtccg gtctctgccg ctctctggat
     1501 gggctgcgct gtgacggccg acggtcggtg tgggcaaggc ccgccccggg gtcgttttca
     1561 gcgccgtgcc catcccggcg ccgccgtggc aataaaaaaa cagacggggc tgggccccgt
     1621 ctgccgtcta ttctgccccc ggcggcgttg ccggcggggc ctttgctttc tcagcacaat
     1681 atgttaaact agtcccgtgc ccgaggggcc gcctcaactc ttttccccgc ggcgctcggc
     1741 ggcctttttc tccggccctt tcatgcgcag ctcgctggcc ttgatgaggt tcagcaggct
     1801 ggtggcgtaa aggggatact cccgcgacag gctctcggtg gtcatggc
//
LOCUS       DBMDYA010000607         1844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12698_length_1844_cov_1.732645, whole genome shotgun sequence.
ACCESSION   DBMDYA010000607 DBMDYA010000000
VERSION     DBMDYA010000607.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_1844_cov_1.732645"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="ACZPFK_12440"
     CDS             complement(<1..825)
                     /locus_tag="ACZPFK_12440"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1407159.1"
                     /translation="MPAILVIDTGSSSMRGILFDENGEILRKEQRKYFMSVEADGTAE
                     QDPGVFARCLAEICAACAGWARAEGRSIAALSFTSQRSSILPLSRQGQPLCNVITWYD
                     KRSAAICEQIIRSRGEELYALAGMRPTPVLSAPKMAWLRQNRPGVYREADKIVGIHDY
                     LLLLCTGRAVTDATLASRTCLMDIRRLCWSGRLLELFGLDGDKLCALLPPGSVVGSVT
                     RSFAEKTGLAPGTPVVTAGGDQQCSVLGQGLLRAGMAGVTVGTGAYLAAVSDGPVFD"
     gene            1108..>1844
                     /gene="ulaG"
                     /locus_tag="ACZPFK_12445"
     CDS             1108..>1844
                     /gene="ulaG"
                     /locus_tag="ACZPFK_12445"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIR1407160.1"
                     /translation="MSKVDEITRESWIMGAFPEWGTWLNEEIEEEVVRPGNVAMWWLG
                     CTGMWIKSEGGCNLSVDFWAGNGKRTRAGKLMAKGHQMANMCGGRKLQPNLRAIPHVL
                     DPFAVKELDAVVATHYHQDHMDPNLAAHVLRDLPGDIPFIGPLKSVETWVGWGVPRGR
                     CIVVKPGDTVKIKDVELVALDSFDRTCLVTTDGEQDIRNTCPSDMDDKAVNYLIKTSG
                     GNIYHSGDSHYSIYFAKHGKDYDIDVA"
BASE COUNT          398 a          555 c          593 g          298 t
ORIGIN      
        1 gtcaaagacc gggccgtcgc tgacggcggc gagataggcc ccggtgccca ccgtgacgcc
       61 cgccatgccc gcgcgcagca ggccctggcc cagcaccgag cactgctggt ccccgcccgc
      121 cgtgaccacc ggcgtgccgg gggcaaggcc ggtcttttcg gcaaagctgc gggtcaccga
      181 ccccaccacc gaccccggcg gcagcagcgc gcagagcttg tccccgtcca ggccgaacag
      241 ctccagcagg cggccggacc agcacagccg ccggatatcc atcaggcagg tgcggctggc
      301 gagggtggcg tcggtgaccg cgcggccggt gcagagcagc agcaggtagt cgtggatgcc
      361 cacgatcttg tccgcctcgc ggtacacccc gggccggttc tgccgcagcc aggccatctt
      421 gggggccgag agtaccggcg tgggccgcat gcccgccagg gcgtagagct cctccccgcg
      481 gctgcggatt atctgctcgc agatcgcggc agagcgcttg tcgtaccagg tgatcacatt
      541 gcagagcggc tgcccctgcc gcgagagggg caggatggaa gaacgctggg aggtgaacga
      601 gagggcggcg atggagcgcc cctccgcccg cgcccagccc gcgcaggcgg cgcagatctc
      661 cgcaagacag cgggcgaaca cgccggggtc ctgctcggcc gtgccgtccg cctccaccga
      721 cataaaatat ttgcgctgct ctttgcgcag gatttccccg ttctcatcga acaggatgcc
      781 ccgcatgctg gaagaaccgg tgtcgatcac gagaatggcg ggcatggctt catcctccca
      841 tttcctcttt agaatagatg atcttataga tgcaggataa cacgcaagga actaaaaaac
      901 aatcttaaaa agcgcttata tcatgctttt gtgagaataa acaataaaat acgacaaaga
      961 tagttcattt tatagattga atttggggtt aaaagaaaat ataatgagga tgccaacaaa
     1021 aattgagcac aaaacgatca taaacctgcg ttcacgatct tctgcaccga ggagggtgca
     1081 ggggcggcag gcggaggaga gaagcagatg agcaaagtcg atgagatcac gcgggagtcc
     1141 tggatcatgg gcgcatttcc tgaatggggc acctggctga atgaggagat cgaagaggag
     1201 gtcgtccgac cgggcaacgt ggccatgtgg tggctgggct gcaccggcat gtggatcaag
     1261 agcgagggcg gctgcaacct gagcgtggat ttttgggccg gcaacggcaa gcgcacccgg
     1321 gcgggcaagc tgatggcaaa ggggcaccag atggccaaca tgtgcggcgg ccgcaagctg
     1381 cagcccaacc tgcgggccat cccccatgtg ctggacccct ttgcggtaaa agagctggac
     1441 gcggtggtcg ccacccacta tcaccaggac cacatggacc cgaacctggc cgcccatgtg
     1501 ctgcgggacc tgccggggga catccccttt atcgggccgc tgaaatcggt ggagacctgg
     1561 gtcggctggg gggtgccgcg ggggcgctgc atcgtcgtga agcccggcga cacggtcaaa
     1621 atcaaggacg tggagctggt ggccctggac tctttcgacc gcacctgtct ggtgaccacc
     1681 gacggcgagc aggatatccg caacacctgc cccagcgata tggacgacaa ggcggtcaac
     1741 tacctgatca aaaccagcgg cggcaatatc taccacagcg gcgacagcca ctattccatt
     1801 tattttgcca aacacggcaa ggactatgac atcgacgtgg cgtt
//
LOCUS       DBMDYA010000608         1844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12699_length_1844_cov_1.555364, whole genome shotgun sequence.
ACCESSION   DBMDYA010000608 DBMDYA010000000
VERSION     DBMDYA010000608.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12699_length_1844_cov_1.555364"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="ACZPFK_12450"
     CDS             complement(<1..663)
                     /locus_tag="ACZPFK_12450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1407161.1"
                     /translation="MNSIKSFRVSGKEQLLLFNSARFLLFFPAVTLIYFIIPHRWRYL
                     WLLGASYYFYMCWSPRYALLMAASTLITWLSGLGLERAERFWGPGRRTRWKKLFVGFS
                     FALNLGILFVFKYLDFALATLQRLLAAVGAQVALPALDLLLPVGISFYTFQALSYTVD
                     VYRGDVKAEHNLLRYALFVSFFPQLVAGPIERSKELLGQLQEKHGFDFARAKSGVLLM
                     LWG"
     gene            complement(660..878)
                     /locus_tag="ACZPFK_12455"
     CDS             complement(660..878)
                     /locus_tag="ACZPFK_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407162.1"
                     /translation="MSTIANNVRRIIRQRGLKQCAVAAMAGYDARRFSSLLRGKRSMT
                     DEDVQAIARALSVTPNELFETRPQDKTA"
     gene            1029..1382
                     /locus_tag="ACZPFK_12460"
     CDS             1029..1382
                     /locus_tag="ACZPFK_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407163.1"
                     /translation="MGIGKRVKEARERLHLTQKELADQVGVTSSAIANYESEVSHPKE
                     PVLYALLGALQVDANFLFQDCIDIKTHFDCTLEEQDLLRKFRALDSYAQETLKLLLER
                     EYGRLSPTDRRETGG"
BASE COUNT          402 a          560 c          526 g          356 t
ORIGIN      
        1 gccccacagc atgagcagca cgccggattt tgcgcgcgca aagtcaaagc cgtgcttttc
       61 ctgcagctgc cccagcagct ccttgctgcg ttcgatgggg cccgccacca gctgaggaaa
      121 aaagctgacg aacagggcgt agcgcagcag attgtgctcc gctttcacgt cgccccggta
      181 gacgtccacc gtgtagctca aggcctgaaa cgtgtaaaag ctgatcccca ccggcagcag
      241 caggtcaagg gcgggcagcg cgacctgcgc ccccaccgcc gccagcaggc gctgcagggt
      301 cgccagggca aaatccaaat atttaaaaac aaacaggatc cccaggttca gcgcgaagct
      361 aaaccccaca aaaagctttt tccaccgcgt cctgcgcccc ggaccccaaa agcgctcggc
      421 gcgctccagg cccaggcccg agagccaggt gataagcgtc gaggcggcca tcagcagcgc
      481 atagcggggg ctccagcaca tataaaaata gtagctggcc cccaaaagcc acaaatagcg
      541 ccagcgatgg ggtatgataa aataaatcag cgtcaccgcc ggaaaaaaca gtaaaaaacg
      601 tgcggaattg aatagcaaca gctgctcctt tcccgatacc cgaaagcttt tgatggaatt
      661 caagccgtct tgtcctgggg ccgcgtctca aacagctcat tcggcgtgac gctcagcgcg
      721 cgggcgatcg cttgcacgtc ttcatccgtc atacttctct tgccgcgcag caggctgctg
      781 aaccggcggg catcgtaacc ggccatggcg gccaccgcac attgcttcag gccccgctgc
      841 ctgatgatgc gccgcacatt attggcgatc gtcgacattt tttgcgcctc cttgtcaaga
      901 ttcttgatat tatctttatt ttatatcaag aatctttgta tttcaagagg tctttctaaa
      961 gtttcttgag gtttttgctt gactagtatc tattttccat tatcataagc tcagggggga
     1021 tcaagggtat gggaatcggc aaacgagtaa aagaggcgcg ggagcggctg catctgaccc
     1081 agaaggagct ggcggaccag gttggggtga ccagctccgc catcgccaat tatgagagcg
     1141 aggtcagcca tccaaaggag ccggtccttt acgcgcttct cggcgctttg caggtagacg
     1201 ccaattttct gtttcaggac tgcatcgata tcaagacaca ttttgattgc acgctggagg
     1261 agcaggacct gctgcgcaaa ttccgcgcgc tggacagtta tgcgcaggag accctgaagc
     1321 tgctgctgga acgggagtac ggccgcctct cccccacgga ccggcgggag acgggcggct
     1381 gagcgccccg cacgcggcgg agcggtgtcc ccggcagcgg agaatagtta aaaacagccc
     1441 ccggcggatc ggaaaacaac attccgatcc gccgggggct gttctggccc gcgaagcgcg
     1501 ttcgcctgtt gcgggctccc ccgcggcggg agacgagggg gcggcgcgct ctttgcgcac
     1561 aagatttttc ctctgcgccc cgcgcgcggc ctcacagccg cctcgccgca gcgcgtcttc
     1621 caatgccctg cgctctattg cgcccgcaaa gagcaagtat ggcaaaagaa aaaaggaccg
     1681 atgcaactgc atcggtcctt tttggcaact gatcgttcag cctctggaac agtgattcct
     1741 tagtacatgc cgcccatgga ggggtcgccg ccggccggca tcgggggctc tttctcgggg
     1801 atatccgcga ccaggctctc ggtggtcagc accatctcgg ccac
//
LOCUS       DBMDYA010000609         1838 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12751_length_1838_cov_1.260219, whole genome shotgun sequence.
ACCESSION   DBMDYA010000609 DBMDYA010000000
VERSION     DBMDYA010000609.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_1838_cov_1.260219"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..488)
                     /locus_tag="ACZPFK_12465"
     CDS             complement(108..488)
                     /locus_tag="ACZPFK_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR1407164.1"
                     /translation="MKARLKRLALLFCLVLLGVGVGVLLSRAGLGVPCLFHAATGLDC
                     PGCGVTRMLGALAAGDLAAAWRSNPALLLLSPLLALLALRLAAGWLRTGALRPSRSQC
                     LLLAGCAAALLAFGVLRNLPFYPY"
     gene            1276..1782
                     /gene="purE"
                     /locus_tag="ACZPFK_12470"
     CDS             1276..1782
                     /gene="purE"
                     /locus_tag="ACZPFK_12470"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1407165.1"
                     /translation="MMKKVGILMGSDTDLPVVQKAIDVLRELEVPFEAHVMSAHRTPK
                     AAAEFSEKALENGFGVLIGAAGMAAHLAGALAANTVLPVIGLPIRGGAADGMDALLAT
                     VEMPWGIPVATVAIDGGKNAGLLAAEILAVEDEALRARLRARRDAEAERLRAKDAKLQ
                     QTLADQLK"
BASE COUNT          384 a          546 c          621 g          287 t
ORIGIN      
        1 attaaaataa gttgtttttt aggccattgg cccatccttg gaccacggcc atagaaataa
       61 aggcggataa ctattctgcg caaaaagttg gctgcgcctt ttccttttta atagggataa
      121 aagggcagat tgcgcaaaac gccgaaagcg agcagcgcgg cggcgcagcc cgcgaggagc
      181 aggcattggc tgcgggaggg gcgcagggcg ccggtgcgca gccagcccgc ggcgaggcgg
      241 agcgccaaca gggccagcag gggcgagagc agcagcaggg cggggttgct gcgccaggcg
      301 gcggcgaggt cgcccgcggc gagcgcgccc agcatccgcg tcaccccgca gccgggacag
      361 tccaggccgg tagcggcgtg aaagaggcag ggcaccccca gcccggcgcg agagagcagc
      421 accccgacgc ccacccccag cagcaccagg cagaagagca gcgccaggcg tttcagccgc
      481 gctttcacgg tcgttcaccg ccgcccgccg cgttttgtcc ggctctgcgc cgaatcgggg
      541 cgagagagaa ataaccggca cgggcggtgc tgtctgcgct gaagatcatg ggcggccctc
      601 ctttaaaatg gcgccgaaac ggcgcggtat cggggtgcga aaaacatctt ttttaaggat
      661 atcaaagagc gccccccacg tcaaccaaag acagcgcgcc tgtaaggggc gggtccgtgg
      721 gaaaagggaa gcggacgcgg ggagaatgct tgccccccag cggccgtccc gtgcggaacc
      781 gttgtaaaaa tggataaaga aaaccggcga aaatcgcctt ttccctgtct gtttccccga
      841 aaaaataaaa agtcgccctc agccattgct tttgccggac aaaccctcta atattatatt
      901 gtactttatc tccggcaaag agacggcccg ccgactcttt gccgggctct gttcacagcg
      961 attttgcggt gcgaaaagag gaggggcggc atgcaaaagt gcgattggaa ccagtccggc
     1021 aggtccggcg gtccgggcgc aggggcaagt gcccgctttt tgaacggctt cccggggcgg
     1081 ggagcaacct tttttccccg cgagaacacg accagtgtaa cctgcggcgg catcggcgga
     1141 gtgtaaggct atggcacacg tgtgccatag ccttttttgc tggctgcaag cgggctgcac
     1201 cggggcacaa gggccgcgct gcggcgcgga aaaccacgtc tttacttatt tttaagaaga
     1261 tacagggagg aacagatgat gaaaaaagtt ggtattttga tgggcagcga cacggatctg
     1321 ccggtggtac agaaagcgat cgacgtcctg cgggagctgg aagtcccctt tgaggcgcat
     1381 gtcatgagcg cgcaccgcac ccccaaggcg gcggcggagt tcagcgaaaa agcgctggag
     1441 aacggcttcg gcgtgctgat cggcgccgcg ggcatggccg cccatctggc cggcgcgctg
     1501 gcggcgaaca ccgtgctgcc ggtcatcggc ctgcccatcc gcggcggcgc cgccgacggg
     1561 atggacgccc tgctggcaac ggtcgagatg ccctggggca tccccgtggc cacggtggcc
     1621 atcgacggcg gcaaaaacgc cggcctgctg gcggccgaga tcctggcggt ggaggatgag
     1681 gcgctgcgcg cccgtctgcg cgcccgccgc gacgccgagg ccgagcgcct gcgcgccaag
     1741 gacgccaagc tgcagcagac cctggccgac cagctgaaat gagtgctttt cggcagtatg
     1801 gggcggcgag cccagtacaa gcgggtacaa caaaacgt
//
LOCUS       DBMDYA010000610         1837 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12759_length_1837_cov_1.244240, whole genome shotgun sequence.
ACCESSION   DBMDYA010000610 DBMDYA010000000
VERSION     DBMDYA010000610.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12759_length_1837_cov_1.244240"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="ACZPFK_12475"
     CDS             complement(<1..514)
                     /locus_tag="ACZPFK_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1407166.1"
                     /translation="MIYHPWLCGRPKIEDAKALSEALGLPRLVCDVLVSRGFDTPEKA
                     RAFLSEEAPISPPSLLKNIDAAAQRIRRAVDGGEKIAVFGDYDVDGVCATALMYSYLE
                     SLGAEVYYKLPNRADEGYGLCRASIDLIASRGITLIITVDNGISAVEEIALANQLGLD
                     VVVTDHHTAPQ"
     gene            complement(605..>1837)
                     /locus_tag="ACZPFK_12480"
     CDS             complement(605..>1837)
                     /locus_tag="ACZPFK_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1407167.1"
                     /translation="GARPIVLERGPCIERRAAAVEAFFAGGALDENANIQFGEGGAGT
                     FSDGKLTTRIGDPLCDYVRRTLLEHGAPGEIAWRAKPHIGTDRLRGVITSLRSEIEGL
                     GGEVRFDTRLTGLQIREGRIAGARTTAGLIETDTLVLAVGHSARDTFEMLRSAGVPLE
                     AKPFSVGLRAEHLQAEIDRALYHEAAGHPALPPGEYQLSAHLGGRGVYTFCMCPGGQV
                     VAAASEAGGLCTNGMSFHARDGANANAAVVVGVDGADFGGDPMQAVAFQRQLERAAFA
                     LAGDGTAPAQDMKHFLAGEPGFVQGRVRPTYPRGVLAADLGALLPSELAAALRGGLRA
                     FGARLPGYCADDAVLTGLETRTSSPVRIPRGPAGESVALAGLYPCGEGAGYAGGIVSA
                     AVDGLRAARAILQRRRYD"
BASE COUNT          278 a          666 c          607 g          286 t
ORIGIN      
        1 cctgtggggc ggtatgatgg tcggtcacca ccacatccag gccgagctgg ttggccaaag
       61 cgatttcctc cactgcggaa atgccgttgt ccaccgtgat gatgagggtg atgccccgcg
      121 aggcgatcag gtcgatggag gcgcggcaca gcccatagcc ctcgtctgcg cggttgggca
      181 gcttgtaata gacctcggcc cccaggctct ccagatagct gtacatcagc gccgtggcgc
      241 acacgccgtc cacgtcgtaa tcgccgaaga cggcgatttt ttcgccgccg tccaccgccc
      301 ggcggatgcg ctgcgccgcc gcgtcgatat ttttcagcag tgagggcggc gagatcggcg
      361 cttcctcgct gagaaaagcg cgcgcctttt ccggcgtgtc gaacccgcgg gacaccagca
      421 cgtcgcagac cagcctgggc aggcccagcg cctccgaaag cgcctttgca tcttcaattt
      481 tgggccgtcc acagagccag ggatgataaa tcatgaatat cgtttcttcc ttttttcagc
      541 ttttttcgac tttttagctt tttcggtacg gaggggcgca tttgacctac cgctcggcca
      601 cggctcaatc atagcggcgg cgctgcagga tggcccgggc ggcgcgcagg ccgtccaccg
      661 ccgcactgac gatgccgccc gcatagcccg cgccctcccc gcaggggtaa agccccgcca
      721 gtgcgacgct ctcccctgcg ggcccgcggg ggatgcgcac cgggctgctg gtgcgggtct
      781 caaggccggt gagcaccgcg tcgtcggcgc agtagccggg cagccgcgcg ccgaaagccc
      841 gcaggccgcc gcgcagggct gccgccagct cggacggcag cagcgcgccc aggtctgcag
      901 ccaggacgcc gcggggatag gtggggcgca cccgcccctg cacaaagccc ggctctccgg
      961 ccaaaaagtg cttcatatcc tgtgccgggg ccgtgccgtc cccggccagc gcaaaagccg
     1021 cccgctccag ctgccgctgg aacgcgacgg cctgcatcgg gtctccgccg aaatccgcgc
     1081 cgtccacccc caccaccacg gcggcgttgg cattggcgcc gtcgcgggcg tgaaagctca
     1141 tgccgttggt gcaaaggccc cccgcttcgc tggccgcggc caccacctgg ccgccggggc
     1201 acatgcaaaa ggtatagaca ccgcggccgc ccaggtgggc ggagagctga tactcccccg
     1261 gcggcagggc cgggtggccc gccgcctcgt ggtagagggc gcggtcgatc tcggcctgca
     1321 gatgctcggc gcgcaggccc accgaaaacg gctttgcctc cagcggcacc ccggccgacc
     1381 gcagcatctc aaaggtgtcc cgggccgagt ggcccacggc cagcaccagg gtgtccgtct
     1441 cgatcaggcc cgcagtggtg cgcgcccccg cgatgcggcc ctcccgtatc tgcaagccgg
     1501 tgagacgggt gtcgaagcgc acctcgcccc ccagcccctc gatctcgcta cgcagggagg
     1561 tgatcacccc gcgcagccgg tcggtgccga tgtgcggctt tgcccgccag gcgatctcgc
     1621 cgggtgcgcc gtgctccagc agggtgcgcc gcacatagtc gcagagcggg tcgccgatgc
     1681 gggtggtgag cttgccgtcc gaaaaagtgc ccgccccgcc ctcgccgaac tggatgttgg
     1741 cgttttcgtc cagcgctccg ccggcgaaaa aggcctctac cgccgccgcc cgccgctcga
     1801 tgcaggggcc gcgctcgagc acgatgggcc gcgcgcc
//
LOCUS       DBMDYA010000611         1835 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12780_length_1835_cov_1.593426, whole genome shotgun sequence.
ACCESSION   DBMDYA010000611 DBMDYA010000000
VERSION     DBMDYA010000611.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12780_length_1835_cov_1.593426"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="ACZPFK_12485"
     CDS             complement(<1..352)
                     /locus_tag="ACZPFK_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407168.1"
                     /translation="MKEEKTKSKLPGLTELFRFTVVIGLLRGLWTTCQMFVDYAVFWG
                     TAYAGLAAIDIGLRIIELVLYAGMLVYIALRKRTFLICFWADVGCRVMTLLVVLFAGG
                     SAAEFLTPLILPLVW"
     gene            complement(451..>1835)
                     /locus_tag="ACZPFK_12490"
     CDS             complement(451..>1835)
                     /locus_tag="ACZPFK_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1407169.1"
                     /translation="SGAAGPELLLDAARRLTGTRRLFPVFCGSALTGEGVAALLDGLD
                     LLLSTRYEALEAAPFSARVYQIRHDPKGVRLAFLKVLSGRLPVRAAVGAEKVSELRVY
                     TGPRFAPIPEALAGQVVAAAGLSSPAGALLGPGGAGKAPVFTPVLAARVAAEPADETA
                     VRRAFTLLADEDPALDARWDPALRALEVRVMGRVQLEVLQALALERFGLQVRFGPPAV
                     RYLETIAAPAVGIGHFEPLRHYAEVWLRLSPGPRGSGVTFESACSTDLLDANFQNLIR
                     THIFEKEHRGVLTGAPVTDLKVTLLTGRSHLKHTEGGDFREAVYRAVRQGLASAKSVL
                     LEPFYDFTVTVPAEAVGRVLADLQRRQGSFSPPQTEGGFAALKGRGPAACFLDYAAEL
                     PAFTGGRGSFTAVFGGYEPCHNAEEVIAAAGYDFERDTENPADSVFCSHGAGYPVSWD
                     EVPSHAHCAL"
BASE COUNT          356 a          632 c          601 g          246 t
ORIGIN      
        1 cccagacgag ggggagaatc agcggcgtca aaaattccgc agcgctgccg cctgcaaaca
       61 ggacgacgag cagggtcatc acccgacagc cgacgtcggc ccaaaaacaa atcagaaaag
      121 tgcgtttgcg cagggcgatg tagaccagca tccccgcata cagaacaagc tcgataatac
      181 gcagcccgat atctatagct gcgagccctg catatgcggt tccccagaaa acagcgtaat
      241 ccacgaacat ctggcaggtt gtccacagcc cccgcagcag accgatcacc acagtaaaac
      301 ggaacagttc ggtgaggccg ggcagcttcg acttcgtttt ctcttctttc atgattttcc
      361 tcctgtttat tctctgagat caaaagccgc ggccgcccgc ggcaatgtgc cgaagcagtg
      421 aaaagaggag catcccccat gtccggcgcc tcacagggcg cagtgggcgt gagagggcac
      481 ctcgtcccaa ctcacggggt aacccgcccc gtgagagcaa aagacggagt ccgccgggtt
      541 ttcggtgtcg cgctcaaagt cgtagcctgc ggcggcgatc acctcctccg cgttgtggca
      601 gggctcatag ccaccgaaga cagcggtaaa gctgccgcgc ccgcccgtga aagcgggcag
      661 ctcggcggcg taatccaaaa agcaggccgc ggggccgcgg cccttgagcg cggcaaagcc
      721 cccctccgtc tggggtgggg aaaaactgcc ctgccgccgc tgaagatccg ccagcacccg
      781 gcccaccgcc tctgcgggca cggtgacggt gaaatcgtaa aagggctcca gcagcacgct
      841 tttcgccgag gcaagaccct ggcggacggc gcggtagacc gcctcgcgga aatcgccgcc
      901 ctcggtgtgc ttgagatggc tgcggccggt gagcagcgtc accttgaggt cggtgacggg
      961 cgcgccggtg agcacgcctc ggtgctcctt ttcaaagata tgggtgcgga tgaggttttg
     1021 aaaattggcg tccagcaggt cggtggagca ggcgctctca aaggtgacgc cgctgccccg
     1081 ggggccgggg gagagccgca gccagacctc ggcgtagtgc cgcagcggct caaagtggcc
     1141 gatgcccacc gcgggcgcgg cgatggtctc gagataccgc accgcgggcg ggccgaagcg
     1201 cacttgcagc ccaaagcgct ccagcgccag ggcctgcagg acctccaact gcacgcggcc
     1261 catcacccgc acctccagcg cgcgcagcgc cgggtcccag cgggcgtcca gggcggggtc
     1321 ctcgtccgcc agcagggtaa aggcccgccg caccgccgtc tcgtccgcgg gctccgccgc
     1381 tacccgcgcg gccagcaccg gggtgaacac cggcgctttg cccgcgccgc cgggccccag
     1441 cagcgcgccg gccggggacg acagccccgc cgccgcgacc acctgcccgg ccagggcctc
     1501 aggtatgggg gcaaaacggg ggccggtata aacgcgcagc tcgctgacct tttccgcccc
     1561 gacggccgcc cgcaccggca ggcggccgga caataccttt aaaaaggcca gcctgacgcc
     1621 tttcgggtcg tggcggatct gatagacgcg ggccgagaaa ggggcggcct ccagcgcctc
     1681 atagcgggtg gagagcagca gatccagccc gtccagaagc gcggccaccc cctctccggt
     1741 gagggcgctg ccgcaaaaga cgggaaaaag ccgccgcgtc ccggtcagcc gccgcgcggc
     1801 gtcgagcaaa agctccggcc ccgcagcgcc ggaga
//
LOCUS       DBMDYA010000612         1821 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12893_length_1821_cov_1.669186, whole genome shotgun sequence.
ACCESSION   DBMDYA010000612 DBMDYA010000000
VERSION     DBMDYA010000612.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12893_length_1821_cov_1.669186"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="ACZPFK_12495"
     CDS             <1..256
                     /locus_tag="ACZPFK_12495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1407170.1"
                     /translation="HPGLSPERIARVNGRLQALAAEKGCYYLNLEEALCPGGVLGDAY
                     ASNDGLHLSKEGYMAWLEYLITHAAYDPANPYVGGIDPGA"
     gene            complement(694..>875)
                     /locus_tag="ACZPFK_12500"
     CDS             complement(694..>875)
                     /locus_tag="ACZPFK_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407171.1"
                     /translation="GERRAQSVQSTQNAAPSPPAPRGEGVRFCPANRLYRVGKLGDPC
                     RRGSAASPYKGEGAV"
     assembly_gap    876..885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(926..1378)
                     /locus_tag="ACZPFK_12505"
     CDS             complement(926..1378)
                     /locus_tag="ACZPFK_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1407172.1"
                     /translation="MKLGESLSNARKKSGLSQEEVAEKLGVSRQTISKWETDETLPDL
                     RQSKKLALLYHLSLDELIEFDVELEEIERVIENTSEEKQKEVDWNAVWSKKYPILTLY
                     KQTVRIDDYAPQLREMLARLKMEYGYQDTDALLVLKDILARVWQEKGG"
BASE COUNT          298 a          574 c          556 g          383 t
ORIGIN      
        1 gcacccgggc ctgagcccgg agcgcatcgc ccgggtgaac gggcggctgc aggccctggc
       61 ggcggaaaag ggctgctatt acctgaatct ggaggaggcc ctctgccccg gcggggtgct
      121 cggcgacgcc tatgcctcga acgacgggct gcacctgtcc aaagagggct atatggcctg
      181 gctggagtat ctgatcaccc acgccgccta cgaccccgca aacccctatg tgggcggcat
      241 cgacccgggc gcctgacggg cggatgagac gcggggtccc gcggccgggc cggtaaaagc
      301 aataaaaaca gcctccgctt ggcggaggct gtttttttcg gccattcgtt tcggttgccg
      361 cgcagcggcg agaaacgggg ccatcaagat aagttgtttt ttattgcgaa gctataaaaa
      421 atagccgcag cgaagcggaa gctatttttt cggtcatccg tttcggttgc cgcgcagcgg
      481 cgagaaacgg ggccaccaag ataagttgtt ttttattgcg gagcaataaa aaatagccgc
      541 ttggcggagg ctgttttttt ctgctatccg gccagtcctc tggccggggc catagagata
      601 ggacgctgca aaaaaggttg gcgatctctg cggattgcca acctttttaa agatccggga
      661 cgtcaaatag gcgggacgtc aaaaccgccg ctttcaaact gccccctccc ccttgtaggg
      721 ggacgctgcg ctgccgcgcc ggcaggggtc gcccagcttg ccgaccctgt aaagacggtt
      781 cgcagggcaa aaccgcaccc cctctccccg tggcgcaggg ggcgaggggg cggcgttctg
      841 cgtgctctgg acgctctggg cgcgcctctc cccgtnnnnn nnnnngctgt tttttatccg
      901 caagttccgc cgccgacccg tgccctcagc cgcctttttc ctgccagacc cgggccagga
      961 tgtccttgag caccagcagc gcgtcggtgt cctgatagcc gtactccatt tttaagcgcg
     1021 cgagcatctc cctgagctgg ggggcgtagt cgtcgatccg caccgtctgc ttgtagaggg
     1081 tcaaaatcgg gtattttttg ctccacaccg cgttccaatc cacctctttt tgcttttcct
     1141 cgctggtgtt ttcgatcacc cgttcgatct cctccagctc cacgtcgaat tcgatcagct
     1201 cgtccaggga gagatggtac agcagcgcca gctttttgga ctgccgaaga tcaggcagcg
     1261 tctcgtccgt ctcccatttg gaaatggtct gcctgctcac gcccagtttt tccgcgacct
     1321 cttcctggga caggccgctc ttttttctgg cgttggataa actctcgcct aatttcatgg
     1381 atcgcacctc ccaattgagt atgaggccat tataaaggca gggaagagag aaatccacca
     1441 attttgcctt gcaatttcgc actttttctt gtcacggccg caccgtggct gagaagcggg
     1501 ccggggaaga gccaaagccg cccccctttt ggccgggcca cgccccgcgc ggcggcatcg
     1561 ccttgggctc cgcccgcagg tgaatgcctt aaggggccct gcgagaaccg ggccgcgtcg
     1621 ccgcgctgtg ccgcgccgga cggaaagggg cggccgcttt cgcgcgcacc gccccgggcc
     1681 cccgctctct tctgttttgc cgaacgcccg ccgggggccg cctctgccgc gcgggcacgc
     1741 ggcctttttt gcggccgggc cgggcgttcg ttcagggcgg gagggcggcc gtgtcggctc
     1801 ttttccccgg gcgggccggt c
//
LOCUS       DBMDYA010000613         1818 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12918_length_1818_cov_1.391380, whole genome shotgun sequence.
ACCESSION   DBMDYA010000613 DBMDYA010000000
VERSION     DBMDYA010000613.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12918_length_1818_cov_1.391380"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="ACZPFK_12510"
     CDS             complement(<1..227)
                     /locus_tag="ACZPFK_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407173.1"
                     /translation="MGRSGGGGGGGHGGGFSGGHSGGFSGGGRSSGSFSGGFGGGRSG
                     GGRSYGGSSHHSGPVFGGGYRGPVFINTRRY"
     gene            complement(662..>1818)
                     /locus_tag="ACZPFK_12515"
     CDS             complement(662..>1818)
                     /locus_tag="ACZPFK_12515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1407174.1"
                     /translation="DLRHNPRAWRHSLLSTWGMKRPLQKRLVRRGWLMAAAAVALAVI
                     AAQPIALLAAGGEEWALPFALVCGALSLLLALALVLFLRNERQLAQDMGSLVEQIASV
                     RAGNLSLPLELPADADLAAAAKDLADIQQGMGTALEERIKSERMKVELVANVSHDIKT
                     PLTSIIGYIELLKREEGLPPHVQDYVRILDQKSLRLKTMVQDVVEVSKAASGQLPVHP
                     KPLDLGKLLRQTLADMAAQIDAAPVTVRAEIPEAPVAIRADGERLYRVFQNLIQNALQ
                     YSLAGSRVHVSLSVRDGVACASVKNTSATELPDGVDFTERFVRGDASRTDGGSGLGLS
                     IARSFTEACGGRFAVGTDADLFIATVEFAAGVPLEEEPEAPAPEEKVPEK"
BASE COUNT          307 a          623 c          596 g          292 t
ORIGIN      
        1 taatagcgcc gggtgttgat gaataccgga ccgcggtagc cgccgccgaa caccggcccg
       61 ctgtgatgcg aggagccgcc gtaagaacgg ccgccgcccg agcggccgcc gccgaacccg
      121 cctgaaaagc tgccgctgga gcggccgccc cctgaaaaac cgccgctgtg gccgccggag
      181 aaccctccgc cgtggccgcc accgccgcct ccgcctgatc ttcccacaag aaacctcctt
      241 actgtcgttc caaactcttt gatcgcccct catgaggggg ccgcgccgcg ctgccctgtg
      301 ggcagagcgc gctgggtaac ggtgaggcat cctcgcgccg ccggggaaca ccgccgtgct
      361 gattgccgtc tggtcgaccg ggaggcgttt ggcctccctt accgcttgag ttaattaaac
      421 cataccgggg ggtaaattgc aatgtcgccc ccggcccccc gcagcctgcc cgggacgggg
      481 gcgggcgtgg gggaggcaag ggagctccaa atctcatcca atcggcctca cgctcgttca
      541 aactcttgaa atctcaaaaa aagtaaaaat ggaagccgcg aaaaatgggg gaaaagcgca
      601 aaaaaacagc ggaaacggca gagccgtccc gctgtttttt atgaaatttt tgtctgagaa
      661 attatttttc gggcactttt tcctccggcg cgggggcttc cggctcctcc tccaggggga
      721 cgccggcggc aaattccacc gtggcgataa agaggtcggc gtcggtgccc acggcaaagc
      781 ggccgccgca ggcctcggtg aagctgcggg cgatggacag gcccaggccg ctgccgccgt
      841 cagtgcggct ggcgtccccg cgcacgaacc gctcggtgaa gtccaccccg tcgggcagct
      901 cggtggcgga ggtgtttttg acggaagcgc aggccacgcc gtcccgcacg ctgagcgaga
      961 cgtgcacgcg cgagcccgcc agcgagtact gcagcgcgtt ttggatcagg ttttggaaca
     1021 cgcggtagag ccgttcgccg tcggcgcgga tggccaccgg cgcctcgggg atctccgcgc
     1081 gcaccgtcac cggcgccgcg tcgatctgcg cggccatgtc ggccagggtc tggcgcagca
     1141 gcttgcccag gtccaggggc ttcgggtgca cgggcagctg gcccgacgcc gccttgctca
     1201 cctccaccac atcctgcacc atggtcttca gccgcagaga tttctggtcg aggatgcgca
     1261 catagtcctg tacgtgcggg ggcaggccct cctcccgctt gagcagctcg atgtagccga
     1321 tgatggaggt cagcggcgtc ttgatgtcat gggacacgtt ggccaccagc tccactttca
     1381 tccgctcgct cttgatacgt tcctccagcg cggttcccat gccctgctgg atgtcagcca
     1441 ggtcttttgc cgccgcggcc aggtcggcgt ccgcgggcag ctccagcggc agggacaggt
     1501 tgcccgcccg caccgaggcg atctgctcca ccaggctgcc catgtcctgc gccagctggc
     1561 gctcgttgcg caaaaagagc accagcgcca gcgccagcag caaagagaga gcgccgcaga
     1621 ccagggcaaa gggcagcgcc cactcttcgc ccccggcggc gagcagcgcg atgggctggg
     1681 cggcgatcac cgccagcgcg acggcggccg cggccatcag ccagccgcgc cgcaccagcc
     1741 gcttttgcag ggggcgcttc atgccccagg tggacagcag gctgtgccgc cacgcccgcg
     1801 ggttgtggcg caggtcgt
//
LOCUS       DBMDYA010000614         1815 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_12950_length_1815_cov_1.659277, whole genome shotgun sequence.
ACCESSION   DBMDYA010000614 DBMDYA010000000
VERSION     DBMDYA010000614.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12950_length_1815_cov_1.659277"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="ACZPFK_12520"
     CDS             complement(<1..491)
                     /locus_tag="ACZPFK_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407175.1"
                     /translation="MDWIKGVQRAVDYVEAHITEPINYVEVARQAYSSSFHFQRVFSI
                     LCGFTLGDYIRMRRLSLAGGELAASGALVIDVALKYGYDTPESFSRAFARFHGVTPSQ
                     AKRSGAALKSFSPLSVKLTLDGGSTMNYRIETKDAFQLICKKKRIPAGTELSTEAIQP
                     FWQA"
     gene            complement(696..1124)
                     /locus_tag="ACZPFK_12525"
     CDS             complement(696..1124)
                     /locus_tag="ACZPFK_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIR1407176.1"
                     /translation="MDEKETVFEQLNALLNLYDFNADTLSGYLGLPVEQLKGLARGET
                     GTLPEDPDRCFGVFVRIQLLFACAAEEKDLKLAAFLQVLLSHHRLSKKTLAKMAGVEP
                     GDIDALLARPGRINAETKYKIAVTAMALRFFLKDGEPPLP"
     gene            complement(1205..>1815)
                     /locus_tag="ACZPFK_12530"
     CDS             complement(1205..>1815)
                     /locus_tag="ACZPFK_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407177.1"
                     /translation="VENYVYMTGELYEEQFGRHPDYTLVAAHTADASQQARDALSAEL
                     FDLDCVSGVSFTDDVKTSFSGMMQKIDTIVVVLIVCAGLLAFVVLYNLTNINITEREK
                     EIATIKVLGFFDKEVSAYVYRESAVLSLIGTLAGLVLGVFLHMFVIYTVEVDAVMFGR
                     SIKPLSYLFAALLTLLFSLLVNLVMHRKLKKISMVESMKAPE"
BASE COUNT          348 a          565 c          537 g          365 t
ORIGIN      
        1 gcctgccaga acggctggat ggcctccgtc gacagctctg tgccggcggg gatccgcttc
       61 tttttgcaga tgagctgaaa tgcgtccttg gtctcgatcc tgtaattcat ggtgcttcct
      121 ccgtctaaag tcagttttac ggagaggggg gaaaaggatt tgagcgccgc gccgctgcgt
      181 ttggcctgtg agggcgtcac cccgtgaaag cgggcgaacg cgcggctgaa gctctcgggc
      241 gtgtcgtacc cgtatttgag ggccacgtcg atcaccagcg ccccgctggc cgcaagctcg
      301 cctccggcca gggagagccg ccgcatgcgg atgtagtcgc ccagcgtgaa accgcacagg
      361 atactgaaca cccgctgaaa gtggaagctg gaggagtaag cttgccttgc cacctccaca
      421 taattgatgg gctcggtgat atgcgcctcc acataatcca ccgcccgctg cacacccttg
      481 atccaatcca ttgtcccgcc cctttccgca tgaccccatg atacagcaaa agcggggcgc
      541 gttcctgaca ttccctgcgc tgcgccgtca tctctgcagg ggcggcaacg cgccctttga
      601 ctgttctctc cgcggtcccc ctcaaaaaga aagggcagcg cgcgtatccc gcggtctgtc
      661 cgcccggggc gcatgtacgg cgcgatggca gtgcttcagg gcaacggcgg ctcgccgtct
      721 ttcaaaaaaa agcgcagcgc catggcggtg acggcgattt tgtatttcgt ttcggcgttt
      781 atccgcccgg ggcgggcgag cagagcgtca atgtctcccg gctccacccc ggccattttg
      841 gcgagagtct tttttgacag gcggtgatga gagagaagca cctgcagaaa ggcggccagt
      901 ttcaagtctt tctcctccgc cgcgcaggca aaaagaagtt gaatcctgac aaagacccca
      961 aaacagcggt ccgggtcttc cggcagggtg cccgtctcgc cccgcgccaa gcctttgagc
     1021 tgctcgaccg ggaggcccag atagcccgag agggtgtccg cattaaagtc atagaggttc
     1081 agcagcgcgt ttaactgctc gaatactgtc tctttttcat ccatagcggc ctcctctaag
     1141 gcttgtttta ccgtttcgtt cgattgcgga gcgccgccca catggcgctc aggacagcgg
     1201 cgcttcactc cggcgctttc atgctctcca ccatactgat ctttttcagc ttgcggtgca
     1261 tcaccaggtt gaccagcagc gagaacagca gggtcagcag cgcggcgaag agatagctca
     1321 gcggcttgat cgagcggccg aacatcaccg cgtccacctc cacggtgtag atgacgaaca
     1381 tgtgcagaaa gacgcccagc accagcccgg ccagcgtgcc gatgagcgag agcaccgccg
     1441 attcgcggta gacgtaggct gagacctcct tgtcgaaaaa gcccagcacc ttgatggtgg
     1501 cgatctcctt ttcccgctcg gtgatgttga tgttggtcag gttgtacagc accacaaagg
     1561 ccagcaggcc cgcgcagacg atgagcacca ccacgatggt gtcgatcttc tgcatcatgc
     1621 cggaaaagct ggtcttcacg tcgtcggtga agctcacgcc ggacacgcag tccaggtcga
     1681 acagctcggc gctcagcgcg tcccgtgcct gctgcgaggc gtcggcggtg tgggcggcca
     1741 ccagcgtgta gtcggggtgc cggccgaact gctcctcata gagctccccg gtcatgtaga
     1801 catagttctc cacat
//
LOCUS       DBMDYA010000615         1797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13096_length_1797_cov_1.242335, whole genome shotgun sequence.
ACCESSION   DBMDYA010000615 DBMDYA010000000
VERSION     DBMDYA010000615.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13096_length_1797_cov_1.242335"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="ACZPFK_12535"
     CDS             <1..224
                     /locus_tag="ACZPFK_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407178.1"
                     /translation="IKNDLSCWPRFSPLSRQPKRMAEKIACGYFLLYRRAGGGAREHI
                     AGILSIALLHAARYNESGTKFSSAEAGTR"
     gene            231..1187
                     /gene="sppA"
                     /locus_tag="ACZPFK_12540"
     CDS             231..1187
                     /gene="sppA"
                     /locus_tag="ACZPFK_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR1407179.1"
                     /translation="MTKKQTWGVVIAAAALVLVGLFGSFVAAVLREMGSSAADYLPVY
                     DSFAVISVVGTIQNTSGDELGLNQPSYHHNATLNYVENLQYDRSNKGIFLYFDTGGGG
                     VYESDELYRALMEYKEATGRPIYAFFGPTAVSGGYYIAMAADYISADRNTTTGSIGVI
                     MSYTNVKGLYDKLGLKSVYITSGRNKSMGASDLDLTDEQRAIYQGVVDEAYDQFVGIV
                     CEGRGMPETTVRKLADGRIYTAKQALANGLIDEVTTLDEALDAFCRQTGASPYYTDFS
                     DATIFDRLLGAVASILPKSDSQVLKELAEADQSGVPMYVYAG"
     gene            1171..>1797
                     /locus_tag="ACZPFK_12545"
     CDS             1171..>1797
                     /locus_tag="ACZPFK_12545"
                     /inference="COORDINATES: protein motif:HMM:NF018028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR1407180.1"
                     /translation="MYTQAEPVFAGFWRRAAALLIDTALLFLLCLPLRLLLGFLGALG
                     IGSQPVFFHYTPANLLLAGAVMLYFSAFEGALGATPGKQMLRMRVVCTRTGYLGWKDA
                     IYRETVGRFLNGLFFIGYLMAGPDREKRTWADRLCDTRVLVLPKKGAPAAVAAAPAAQ
                     PDLSARSAATPPVPPPTPAATAPLREGYGDLSTPGGASQLGPLKAGQPA"
BASE COUNT          319 a          594 c          564 g          320 t
ORIGIN      
        1 caataaaaaa cgacttatct tgttggcccc gtttctcgcc gctgtcgcgg caaccgaaac
       61 gcatggccga aaaaatagcc tgcggctatt ttttattata ccgccgcgcg gggggaggag
      121 caagggagca cattgccggc attttgtcga ttgcgctttt gcacgcggcc cggtataatg
      181 aaagcggtac aaaattttcc agcgcagagg ctggaacgag gtgaaaacaa gtgacgaaaa
      241 aacagacctg gggcgtcgtc atcgcggcgg ccgcattggt gctggtggga ttgttcggtt
      301 cctttgtcgc ggccgtgctg cgggagatgg gctcctcggc ggcggactac ctgcccgtct
      361 acgacagctt tgcggtcatc agcgtggtgg gcaccatcca gaacaccagc ggcgacgagc
      421 tggggctcaa ccagcccagc tatcaccaca acgccaccct gaactatgtg gaaaatctgc
      481 aatacgaccg ctccaacaag ggtatcttcc tctatttcga taccggcggc ggcggggtgt
      541 acgagtcgga tgagctgtac cgcgccctga tggagtacaa agaggccacc ggcaggccca
      601 tctatgcctt tttcggcccc acggctgtca gcggtggcta ttacatcgcc atggcggcgg
      661 actacatctc cgccgaccgc aacaccacca ccggctccat cggcgtcatc atgagctata
      721 ccaacgtcaa gggcctttac gacaagctgg ggctcaagtc ggtctatatc accagcggcc
      781 gcaacaagtc gatgggggcc agcgacctgg acctcaccga cgagcagcgc gccatctacc
      841 agggcgtggt ggacgaggcg tacgaccagt tcgtgggcat cgtctgtgag gggcgcggca
      901 tgcccgagac cacggtgcgc aagctggcgg acggacgcat ctacaccgca aagcaggcgc
      961 tggcaaacgg cctgatcgac gaggtcacca cgctggacga ggcgctggac gccttttgcc
     1021 gccagaccgg cgcctccccc tattacaccg acttttcgga cgccaccatc ttcgaccgcc
     1081 ttctgggcgc ggtggcctcc atcctgccca agagcgacag ccaggtgctg aaagagctcg
     1141 ccgaagccga ccaaagcggg gtgccgatgt atgtatacgc aggctgagcc cgtctttgcg
     1201 ggcttctggc ggcgcgccgc cgcccttttg atcgacacgg cgctgctgtt tctgctctgc
     1261 ctgccgctgc gcctgctgct gggctttctc ggggcgctgg gcatcggtag ccagccggtc
     1321 tttttccact acacccccgc caacctgctg ctggccgggg cggtgatgct gtacttttcc
     1381 gcctttgagg gggcgctggg cgcgaccccc ggcaagcaga tgctgcgcat gcgggtggtc
     1441 tgcacccgca ccggttatct gggctggaaa gacgcgatct accgcgagac ggtgggccgt
     1501 tttctcaacg gcctgttctt catcggctac ctgatggccg ggccggacag ggaaaagcgc
     1561 acctgggccg accgcctctg cgacacccgg gtgctggtgc tgcccaaaaa gggcgcccct
     1621 gcggccgtgg ccgcagcgcc cgcggcccaa cccgatcttt ccgcccgatc ggcggcgacc
     1681 ccgccggtgc ccccgcccac cccggcggcg acggcgcccc tgcgcgaggg ctacggcgac
     1741 ctgtcgacgc cgggcggcgc atcccagctg ggtcccctca aggccgggca gcccgct
//
LOCUS       DBMDYA010000616         1780 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13239_length_1780_cov_1.232877, whole genome shotgun sequence.
ACCESSION   DBMDYA010000616 DBMDYA010000000
VERSION     DBMDYA010000616.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13239_length_1780_cov_1.232877"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            287..661
                     /locus_tag="ACZPFK_12550"
     CDS             287..661
                     /locus_tag="ACZPFK_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1407181.1"
                     /translation="MNLNLSDEKSIYLQIGESVENDILRGILLEEERVPSTNEMARAY
                     SINPATAAKGINLLVEQGILYKKRGIGMFVASGAKGQIIAKRKNSFYDSFIVNLVEEA
                     RSLSISEDELVEMIRRAREEKK"
     gene            711..1607
                     /locus_tag="ACZPFK_12555"
     CDS             711..1607
                     /locus_tag="ACZPFK_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1407182.1"
                     /translation="MVLRCEHVSKEYGGKRVLDDVTLEIEPGRIYGLIGRNGAGKTTL
                     LSILTAQNPATAGAVTLGGQRVWENQRALDHLCFSRELNPVNAFGNAAYKVKEYLRMA
                     AAYYPHWDKAYAERLIALFGLDVKKRISKLSKGMLSMVTIVVALASRADVTILDEPVA
                     GLDIVSRERFYQLLIEDYTETGRTFIISTHIIEEAANVFEKVIILHEGRLRLVEDTAE
                     LVARFWAVSGREDEVDAVCAGLQVHHTDVMGRGKTAYVSTEPGGDIRRHTGGHAVDVA
                     PLSLQKLFVAMCGDETVEGESK"
BASE COUNT          369 a          507 c          593 g          311 t
ORIGIN      
        1 cctgctgaaa cgcgcttttg cggtgcgcgc gccttttttg ccgccgcgca gggcgccgcg
       61 aaagcgggga cgggccgcac cggggcagtg aggccgatgc ggacgcagct gccccgtttt
      121 ttgcacgccg gcggattttg ggcggccggc acgacaaagt gagagcgatg ctgccgccgg
      181 aaaaactttt caaaaaaggg ttgacaaatt accttgttag atatatagtt aattacacaa
      241 gtaatgaacc tacgaacctc gtgggccgga gggagaggtg aactgtttga acttgaatct
      301 atccgacgaa aaatccatct acctgcagat cggcgaaagc gtcgaaaacg atatcctgcg
      361 gggcattttg ctggaggaag agcgggtgcc cagcaccaac gagatggcgc gggcctattc
      421 catcaacccg gccaccgcgg ccaagggcat caacctgctg gtggagcagg gtatcctgta
      481 caagaaacgg gggatcggca tgttcgtcgc atcgggcgca aaggggcaga tcatcgccaa
      541 gcgcaaaaac agcttttacg acagttttat cgtcaacctg gtggaggagg cgcgcagcct
      601 ctcgatcagc gaggacgagc tggtggagat gatccgccgg gcaagggaag agaaaaaata
      661 acacgataac acgattcatt tgggaggaag ctatggaagc gaaaaacgat atggtactgc
      721 gctgcgagca tgtcagcaag gagtacggcg gcaaacgggt gttggacgat gtcacactgg
      781 agatcgagcc gggccgcatc tacggcctga tcggccgcaa cggggcgggc aagaccacgc
      841 tgctgtccat cctcaccgca cagaaccccg ccaccgccgg cgccgtcacc ctgggcggcc
      901 agcgggtgtg ggagaatcag cgggcgctgg atcacctgtg cttttcccgg gagctgaacc
      961 cggtcaacgc tttcggcaat gcggcttata aagtgaaaga gtacctgcgc atggcggcgg
     1021 cctattatcc gcactgggac aaggcctacg ccgagcggct gatcgccctg ttcgggctgg
     1081 atgtgaaaaa acgcatctcc aagctgagca agggcatgct gtcgatggtc accatcgtcg
     1141 tcgccctggc cagccgcgcg gacgtcacca tcctggatga gccggtggcc gggctggaca
     1201 tcgtctcccg cgagcggttc tatcagctct tgatcgagga ctacaccgag accggacgca
     1261 ctttcatcat ctccacccac atcatcgaag aggcggcgaa cgtctttgaa aaggtcatca
     1321 tcctgcacga ggggcggctg cgcctggtgg aggacaccgc cgagctggtg gcccgctttt
     1381 gggcggtcag cggccgcgag gacgaggtgg acgcggtttg cgccggcctg caggtgcacc
     1441 acaccgacgt gatgggccgg ggcaagacgg cctatgtctc caccgagccg ggcggcgata
     1501 tccgccgcca taccggcggg catgcggtgg acgtggcgcc gctcagcctg caaaagttgt
     1561 tcgtggccat gtgcggcgac gagacggtgg agggggagtc aaaatgagag attcgctttt
     1621 gcgcaacctg cgcggccagg ggcgcgccaa cgccctggcg gcgggcgcgg tgctgctgac
     1681 ggtgctgggg ctgtgcctgt tctcggcgct caccgcgccg gtggacgcca tcgccgagac
     1741 ggtgtctcag aactttgcga tctatgtgcc gctggcctgc
//
LOCUS       DBMDYA010000617         1779 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13252_length_1779_cov_1.182360, whole genome shotgun sequence.
ACCESSION   DBMDYA010000617 DBMDYA010000000
VERSION     DBMDYA010000617.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_1779_cov_1.182360"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(251..514)
                     /locus_tag="ACZPFK_12560"
     CDS             complement(251..514)
                     /locus_tag="ACZPFK_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1407183.1"
                     /translation="MYVKEVTVENQVGLHARPATFFIQKANEFKSSIWVEKEERRVNA
                     KSLLGVLSLGIVGGTTIRVIADGADEQQAVEGLIKLVNSGFSE"
     gene            complement(594..728)
                     /locus_tag="ACZPFK_12565"
     CDS             complement(594..728)
                     /locus_tag="ACZPFK_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407184.1"
                     /translation="MDLTNDVENFVVRLYKALRPRILDFSELMKMPGRGIILLTGWKY
                     "
     gene            complement(792..1532)
                     /gene="trmD"
                     /locus_tag="ACZPFK_12570"
     CDS             complement(792..1532)
                     /gene="trmD"
                     /locus_tag="ACZPFK_12570"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1407185.1"
                     /translation="MRIDIATLFPEMCEAVLSESIIGRARRKGSIEMYCHNIRDYTLD
                     KQKRVDDYPYSGGHGMILQAQPIYLCCKAIEAQQQVKPHVAFLTAGGKTFTQQRAREL
                     AQLPSLTLVCGHYEGIDERVLEELADEEISLGDFVITGGELAALVVADSVARLCDGVL
                     SSPEGYQDESFYSGLLEYPQYTRPEVWHGRRVPPVLLSGHAKKIAEWKQEQSLQRTLE
                     RRPDLLERRGGPAVPPCAAPAKEKNDSD"
     gene            complement(1522..>1779)
                     /locus_tag="ACZPFK_12575"
     CDS             complement(1522..>1779)
                     /locus_tag="ACZPFK_12575"
                     /inference="COORDINATES: protein motif:HMM:NF017088.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1407186.1"
                     /translation="DVDLPEGTWFIQDIIGLRVLDAVTGEEYGTVADVTNAGAGDIYE
                     IRTPDGRSVLFPAVPEFLAQVSPAEGFVKIRPIGGMFDDAD"
BASE COUNT          329 a          582 c          497 g          371 t
ORIGIN      
        1 gccttgccga tatagatgat ctcgcccgtc ttgtccttga tgatatagac gcccggcagc
       61 agcggcaagc gctttgcctt ttcatagagc tcctgtttgg tcatccgccg cccgcctttc
      121 tcgtttttgt ccccgccctt gcagccgccg cgcgcccctt agtgtagtat gcgcacaccc
      181 ggcaaaaaaa agcgccgccc cgcggacgga gaccgtccgc gggcagcgcc gcacagttgc
      241 tgaaaagacc ttactcgctg aagccggagt tcacgagctt gatgaggccc tcgacggcct
      301 gctgctcgtc ggcgccgtcc gcgatgaccc ggatggtggt gccgcccaca atgcccagcg
      361 acaatacgcc caaaaggctc ttggcattga cgcggcgctc ctctttctcg acccagatgg
      421 aggatttgaa ctcattggct ttctgaataa agaaagtcgc aggacgagcg tgcaggccca
      481 cctgattctc cacggtaact tctttcacat acataataag aacgctccta ttccagaatt
      541 ttctaaataa gaaaaatgtt ttaagctgta atctgatatg gtattatcat agctcaatat
      601 ttccaacccg tcaacaaaat aatccccctg cccggcattt tcatcagttc gctaaaatcc
      661 aaaatgcgcg gacgcagcgc tttgtataat ctcaccacaa aattttcaac atcattggtc
      721 aaatccacaa gttttagcgt ccaatttttg gggtttttgt ctaggggtaa caggcagtgc
      781 agcttttgtg ctcaatcgct gtcgtttttc tcttttgcgg gagcggcgca gggcggcacg
      841 gcgggtccgc cccggcgctc cagcaggtcc ggccgccgct ccagcgtgcg ctgcagcgat
      901 tgctcctgct tccactcggc gatctttttg gcgtggccgg acagcagcac cgggggcacc
      961 cggcggccgt gccagacctc gggccgggtg tactgggggt actccagcag gccgctgtaa
     1021 aagctctcgt cctggtaacc ctcggggctg gacagcacgc cgtcgcacag gcgcgccacg
     1081 ctgtcggcca ccaccagggc ggccagctcg ccgccggtga tgacaaagtc gcccaggctg
     1141 atctcctcgt cggccagttc ctcgagcacc cgctcgtcga tgccctcgta atggccgcag
     1201 accagcgtca ggctgggcag ctgggccagc tcccgcgccc gctgctgggt gaacgtcttg
     1261 ccgcccgcgg tgagaaaggc cacatggggc ttcacctgct gctgcgcctc gatggccttg
     1321 cagcacagat agatcggctg ggcctgcaaa atcatgccgt ggccgccgct gtagggatag
     1381 tcgtccaccc gcttctgctt gtccagcgtg tagtcgcgga tgttgtggca gtacatctcg
     1441 atgctgccct tgcggcgggc gcggccgatg atgctctcag agagcaccgc ctcgcacatc
     1501 tcggggaaga gggtcgcgat atcaatccgc atcgtcgaac atccccccga tcggccgtat
     1561 cttcacaaag ccctccgccg ggctgacctg cgccaaaaac tccggcacgg cggggaacag
     1621 cacgctgcgc ccgtccggcg tgcggatctc gtagatatcc cccgccccgg cgttggtgac
     1681 gtccgccacc gtaccgtact cctcgccggt gaccgcgtcc agcacccgca ggccgatgat
     1741 gtcctggatg aaccaggtgc cctcgggcag gtccacatc
//
LOCUS       DBMDYA010000618         1778 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13265_length_1778_cov_1.070364, whole genome shotgun sequence.
ACCESSION   DBMDYA010000618 DBMDYA010000000
VERSION     DBMDYA010000618.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13265_length_1778_cov_1.070364"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(144..929)
                     /locus_tag="ACZPFK_12580"
     CDS             complement(144..929)
                     /locus_tag="ACZPFK_12580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1407187.1"
                     /translation="MEREDIDGGRRFDWSDASADYAKYRDIYPPAFYQYLRAQGIGKR
                     GQRLLDLGTGTGVLPRSLYPCGAKFVGVDSAEGQIAQARALAERAGMEIDFLCLPAEG
                     IRFAAGSFDGATACQCLMYFDHAALAPRLAHVLKEGGFFAILYMAWLPEEDEVARQSE
                     ELVLRYNPGWTGGGEVRRPIQVPREYGAQFELERSDLFDLQVPFTRESWNGRMKACRG
                     IGASLSVREAQAFEREHLALLERIAPPEFTVRHYTAAAILRKR"
     gene            1567..>1778
                     /locus_tag="ACZPFK_12585"
     CDS             1567..>1778
                     /locus_tag="ACZPFK_12585"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1407188.1"
                     /translation="MERLYIRIGGCVQGVGYRYYATYSARRLGLTGWVRNREDGDLEL
                     EAQGTPDALEAFCDAMRRGPHYAEVQR"
BASE COUNT          342 a          561 c          508 g          367 t
ORIGIN      
        1 tattgcggag caataaaaaa tagccgcagc aaagcggagg ctatttttca gctatccgtt
       61 tcgcttgccg cgccagcggc gagaaacagg gccattgaga taggccgttt ttcattgcaa
      121 agtaataaag agctgaaaca gctctatctt tttcgcaaga tggccgccgc ggtgtagtgg
      181 cgcaccgtaa attcgggagg cgcgatccgc tccagcagcg ccaggtgctc ccgctcgaaa
      241 gcctgtgcct cccgcacgga aagggaggcc ccgatcccac ggcaggcttt catgcggccg
      301 ttccagctct cacgggtaaa ggggacctgc agatcaaaaa ggtcgctgcg ctcaagctca
      361 aactgcgcgc cgtattccct gggcacctgg atgggcctgc gcacttcccc gccgccggtc
      421 cagccggggt tataccgcag caccagctcc tcactttggc gggcaacctc gtcctcttcc
      481 ggcagccagg ccatgtagag gatggcaaaa aatccgccct ctttcaaaac gtgggccagc
      541 cgcggcgcga gcgccgcgtg gtcaaaatac atcagacact ggcaggcggt cgcgccgtca
      601 aagctccccg ccgcaaagcg gatcccctcg gcgggcaggc acaaaaaatc aatctccatc
      661 cccgcccgct ctgccagcgc ccgcgcctgg gcgatctgcc cctcggcgct gtccaccccc
      721 acaaatttag ccccgcaggg atagaggctg cggggcagca ccccggtccc cgtgcccagg
      781 tccagcagac gctgtcccct tttgccgatg ccctgcgccc gcagatattg ataaaaagcg
      841 ggcggataga tatcccggta tttcgcatag tcggcgctgg cgtcgctcca gtcgaatcgc
      901 ctgcccccgt cgatgtcctc tctctccatt tcgctccccc tttcccgttg gtgccatgca
      961 gcgctttctc cgtttttccg gcgcgctttt tgcgcgattg atcgcgttat cttgacgacc
     1021 cccgagagaa tcagccggac ggccgaaaaa acagcctgaa gctattttca catcctcaag
     1081 aggaatcaaa aaagcatcgt tgctgctttc ttcccaggta ctgctttaca agatttctcc
     1141 ggtctccttg ctcacgggtc ccagccgccc ttttcccgcg cagctgaaaa agccgggtcg
     1201 cttttagaac cgccggcccc atgggcacgc ggagcgggcg gccgtgcgca gagggactga
     1261 accgtacggg ctgggtccag ctttagttca agcggcaatg cttgattttt caaagcagga
     1321 gatcgtgcgg atccctctct ttccgcacgc cgtcaagccg ggcaaacaaa aaaggctcaa
     1381 aagggttgtg cctttttgcc gccgattcgg ctttgtggga tatcgtttgg gccgcaaccg
     1441 gcgtgtttct ctcactttaa agggaaacag gagtgttgtc aagctctgcc gttctccgga
     1501 tttgcctcgc gccgcggcgc atgctataat cagagacaag gtttttggtg aaatggaggt
     1561 atccgggtgg aaaggctgta tatccgtatc ggcggctgtg tgcagggggt gggctatcgc
     1621 tattatgcca cctattccgc ccggcggctg ggccttaccg gctgggtgcg caaccgggag
     1681 gacggcgatc tggagctgga agcccagggc actcccgacg ctctggaggc attctgcgac
     1741 gccatgcgcc gcggccctca ttatgccgag gtccagcg
//
LOCUS       DBMDYA010000619         1769 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13347_length_1769_cov_1.967626, whole genome shotgun sequence.
ACCESSION   DBMDYA010000619 DBMDYA010000000
VERSION     DBMDYA010000619.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13347_length_1769_cov_1.967626"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="ACZPFK_12590"
     CDS             <1..270
                     /locus_tag="ACZPFK_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407189.1"
                     /translation="KNSRYAAIFLFECSLSQLYLCRSERSKALPTLCIRPDSNTKKDG
                     AESVVLFMSLTYPRQTVSLLQLCPEEASRVKALFFAPYPYSPRST"
     gene            complement(249..914)
                     /locus_tag="ACZPFK_12595"
     CDS             complement(249..914)
                     /locus_tag="ACZPFK_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR1407190.1"
                     /translation="MERPVKDVGEQRAQEVRLDKPVYQLIALDVAENIISGRYQVGEK
                     VHGRSTLASRYNVSPETVRRAMFLLKDMEILEMKQGSGIFIKNVEKAQAFVQKFKNAC
                     AADELRANLSDLLSQRDALEQKISSSMMNLIDSYERFESANPLTPFELPIREGDALTG
                     KNLADVNFWHNTGATIIAIRRDGGLMLSPGPYAEFLPGDVFLLVCDAESFARAKNYVE
                     RGE"
     gene            complement(920..>1769)
                     /gene="asnS"
                     /locus_tag="ACZPFK_12600"
     CDS             complement(920..>1769)
                     /gene="asnS"
                     /locus_tag="ACZPFK_12600"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1407191.1"
                     /translation="DPEDPPRDADGKVDYGSDFFGRQTNLTVSGQLEAEGMALAFGKV
                     YTFGPTFRAENSNTPRHAAEFWMIEPEMAFADLTDYMDNAEAMIKSVVRHLLASCPDE
                     MAFFNQFYDKGLLERLNALAGSEFARVSYTDAVKILQENNDRFEYKVEWGTDLQTEHE
                     RFLTEQVYKRPVFVTDYPKEIKSFYMRQNDDGKTVAAADMLVPGVGELCGGSQREERL
                     DRLEARMDELGMKKDDYQWYLDLRRFGSAKHAGYGLGFERLIMYVTGIPNIRDVLPFP
                     RTAGGF"
BASE COUNT          296 a          580 c          504 g          389 t
ORIGIN      
        1 aaaaatagcc gctatgcggc tattttttta tttgaatgct ctctttcgca gctatacctc
       61 tgtcgaagcg aaaggtcgaa ggctcttccg acactgtgca tcagacccga cagcaacaca
      121 aaaaaggacg gcgccgaaag cgtcgtcctt tttatgtctt taacctatcc ccgccaaaca
      181 gtttctcttt tgcagctctg ccctgaagaa gcctcccgcg tgaaagccct gttttttgct
      241 ccctatcctt attccccgcg ctccacatag ttttttgccc gagcgaagct ctcggcgtcg
      301 cacaccagca aaaatacgtc ccccggcagg aactcggcgt aggggcccgg ggagagcatc
      361 agcccgccgt cgcggcggat ggcgatgatg gttgcccccg tgttgtgcca gaagttgacg
      421 tccgccaggt ttttgccggt gagcgcgtcc ccctcgcgga tgggcagctc aaagggggtg
      481 agcgggtttg cgctctcaaa ccgctcatag ctgtcgatga ggttcatcat cgagctgctg
      541 atcttctgct ccagggcgtc ccgctggctg agcagatcgc tcaggtttgc ccgcagctcg
      601 tccgcggcgc aggcgttttt aaacttttgc acaaaggcct gcgccttttc cacatttttg
      661 atgaagatgc cgctgccctg cttcatctcc aggatttcca tatccttgag caggaacatc
      721 gcccgccgca ccgtctcggg cgagacgttg tatctgctgg ccagggtgga gcgcccatgg
      781 accttttccc ccacctgata gcggccgctg atgatgtttt ccgccacgtc cagcgcgatg
      841 agctggtaga ccggcttgtc cagcctgacc tcctgcgccc tctgttcgcc cacgtctttc
      901 accggccttt ccacggtcgt caaaaaccgc ccgcagtgcg cgggaacggc agcacgtccc
      961 ggatgttggg gatgccggtg acgtacatga tcagccgctc aaagcccagg ccgtagcccg
     1021 cgtgcttggc gctgccgaac cgccgcaggt ccaggtacca ctggtagtca tcctttttca
     1081 tgcccagctc gtccatgcgc gcctccagcc tgtccagccg ctcctcgcgc tggctgccgc
     1141 cgcacagctc gcccacgccc ggcaccagca tatcggccgc ggccacggtc ttgccgtcgt
     1201 cgttctgacg catgtaaaag ctcttgatct ccttggggta gtcggtgacg aagaccggcc
     1261 gcttgtacac ctgctcggtg aggaagcgct cgtgctcggt ctgcaggtcg gtgccccact
     1321 ccaccttgta ctcgaagcgg tcgttgttct cctgcaaaat cttcaccgcg tcggtgtagc
     1381 tcacacgggc gaactcgctg cccgccaggg cgttgagccg ctccagcagg cccttgtcgt
     1441 agaactggtt gaaaaaggcc atctcgtccg ggcagctggc cagcaggtgg cgcaccaccg
     1501 atttgatcat cgcctcggcg ttgtccatat aatccgtcag gtcggcaaag gccatctccg
     1561 gctcgatcat ccagaactcg gccgcgtggc gcggggtgtt gctgttttcg gcgcggaacg
     1621 tggggccaaa cgtgtacacc ttgccaaagg ccagggccat gccctcggcc tccagctggc
     1681 ccgagacggt gaggttggtc tggcggccga agaaatcgct gccgtagtcc accttgccgt
     1741 ccgcgtcgcg gggcgggtcc tccgggtcg
//
LOCUS       DBMDYA010000620         1768 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13362_length_1768_cov_1.209958, whole genome shotgun sequence.
ACCESSION   DBMDYA010000620 DBMDYA010000000
VERSION     DBMDYA010000620.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13362_length_1768_cov_1.209958"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="ACZPFK_12605"
     CDS             complement(<1..151)
                     /locus_tag="ACZPFK_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1407192.1"
                     /translation="MAFTAQDVKALREQTGCGMMDCKKALTDADGDFDKAVELLREKG
                     LAAQTK"
     gene            complement(201..941)
                     /gene="rpsB"
                     /locus_tag="ACZPFK_12610"
     CDS             complement(201..941)
                     /gene="rpsB"
                     /locus_tag="ACZPFK_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR1407193.1"
                     /translation="MAVVSMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKMLDEAYGFVRDLAAEGGTLLFVGTKKQAQDSIREEAERCGMYYVNARWLGGTLT
                     NFRTIRKRIDRLEQLKKMREDGTFDLLPKKEVVKHELEIEKLEKFLGGVKDMNKLPKA
                     MFIVDPHKERIAVSEARKLHIPIIAIVDTNCDPDEIDHVIPGNDDAIRAVRLISGAMA
                     DAVLEGRQGVADAAEAAPAEAAAQTANA"
     gene            complement(1092..1367)
                     /locus_tag="ACZPFK_12615"
     CDS             complement(1092..1367)
                     /locus_tag="ACZPFK_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HIR1407194.1"
                     /translation="MFTLYELCERDVVSVKNGANLGRVDDLRVDETTARVQSLVIYGR
                     LKWFGLLGREEDLSIPWEEIVTIGSDVVLVNTDLPPRERKLKKFLKF"
     gene            complement(1466..>1768)
                     /locus_tag="ACZPFK_12620"
     CDS             complement(1466..>1768)
                     /locus_tag="ACZPFK_12620"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407195.1"
                     /translation="QRVAIARALSMEPEAMLFDEPTSALDPEMVGEVLKVIRDLADTG
                     LTMLIVTHEMAFAREVADRIVFMADGVIVEQGPPEQLFDHPQQERTRQFLSRFRGA"
BASE COUNT          308 a          553 c          512 g          395 t
ORIGIN      
        1 ttttggtctg ggccgcaagg cccttttcac gcagcagctc gaccgccttg tcaaagtcgc
       61 cgtccgcatc ggtcagcgct tttttgcagt ccatcatgcc gcagccggtc tgctcgcgaa
      121 gtgcttttac gtcttgtgca gtgaaagcca taacttttta cctcctacta ttcaatcaac
      181 aagcccagcc cggcagcctc tcaggcgttg gcggtctgcg cggcggcctc ggcgggggcg
      241 gcctcagccg cgtccgcgac gccctggcgg ccctcgagca ccgcgtcggc catggcgccg
      301 gagatcaggc ggacggcgcg gatggcgtcg tcattgcccg ggatcacgtg atcaatctcg
      361 tccgggtcgc agttggtgtc cacgatggcg atgatgggga tgtgcagctt gcgggcctcg
      421 gagacggcga tgcgctcctt gtgcgggtcg acgatgaaca tggccttggg cagcttgttc
      481 atgtctttca cgccgccgag gaatttctcc agcttttcga tctccagctc gtgcttgacg
      541 acctcttttt tgggcagcag gtcaaaggtg ccgtcctcgc gcatcttttt gagctgctcc
      601 agccggtcga tccgcttgcg gatggtgcgg aagttggtca gggtgccgcc cagccagcgg
      661 gcgttcacat aatacatgcc gcagcgctcg gcctcctcgc ggatggaatc ctgcgcctgc
      721 tttttggtgc cgacgaagag cagtgtgccg ccctcggccg cgagatcacg cacaaagccg
      781 taggcctcgt ccagcatctt gacggtcttc tgcaggtcga tgatgtagat gccgttgcgc
      841 tcggtgaaga tatacttctt catcttgggg ttccaccggc gggtctggtg tccaaagtgg
      901 acgccggctt cgagaagctg tttcattgat acgactgcca taattctttg tcctcctaaa
      961 aattgttctg cctccgcatt gcctgtatgc ttcgccaccc aaaacggcgt tttgggcaca
     1021 cgcgggcaaa gcaatgcgtg cgtattgccg agttagtgta ccatattttg taacacatta
     1081 caagtttttt ctcaaaattt caggaatttt ttcagcttgc gctcccgggg cggcagatcg
     1141 gtgttcacca gcaccacatc gctgccgatg gtgacgatct cctcccacgg gatcgagagg
     1201 tcctcttctc tgcccagcag cccaaaccac ttcagccgcc cgtagatcac cagcgattgc
     1261 acccgcgccg tcgtctcgtc cacgcgcaga tcatccaccc ggcccaggtt tgccccgttt
     1321 ttgacgctca ccacatcccg ctcgcacagc tcatacagtg tgaacataca gcatcacgcc
     1381 cctctgtcaa atcagtactt tatatttacg gcgcgggggg caaaacaaga acccgggcgc
     1441 gtggcccggg cttttgaaag tgttttcacg cgccccggaa gcgggacaaa aactggcggg
     1501 tgcgctcttg ctgcgggtgg tcgaacaact gctcgggcgg cccctgctcc acgatgaccc
     1561 catcggccat aaagacgatg cggtcggcga cctcgcgcgc aaaagccatc tcatgggtga
     1621 cgatgagcat ggtcaggccg gtgtccgcca agtcgcggat gaccttcagc acctcgccca
     1681 ccatctccgg gtccagggcg ctggtgggct cgtcaaagag catggcctcc ggctccatcg
     1741 agagggcgcg ggcgatggcg acccgctg
//
LOCUS       DBMDYA010000621         1763 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13396_length_1763_cov_1.300241, whole genome shotgun sequence.
ACCESSION   DBMDYA010000621 DBMDYA010000000
VERSION     DBMDYA010000621.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13396_length_1763_cov_1.300241"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="ACZPFK_12625"
     CDS             complement(<1..518)
                     /locus_tag="ACZPFK_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1407196.1"
                     /translation="MAERLLNLDSIDDALAVLGSGDENIKKIEREFGVCAVCRGTEIK
                     VTGEEAAVDGAGRAIDAMLQLWRSKTPLDEQALHYCISLAASGDEGRVKELTGDFIAL
                     TAKGKPVRPKTLGQKAYIDAIRANTITFGVGPAGTGKTYLAVAMAVRAFKAHEIARII
                     LTRPAVEAGEKLG"
     gene            complement(784..1728)
                     /locus_tag="ACZPFK_12630"
                     /pseudo
     CDS             complement(784..1728)
                     /locus_tag="ACZPFK_12630"
                     /inference="COORDINATES: protein motif:HMM:NF018591.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
BASE COUNT          275 a          620 c          519 g          349 t
ORIGIN      
        1 cccagctttt cccccgcctc caccgccggg cgggtgagga tgatgcgggc gatctcgtgc
       61 gccttgaacg cccgcacggc catcgccacc gccagatagg tcttgccggt gcccgcgggc
      121 cccaccccaa aggtgatggt gttggcgcgg atggcgtcga tataggcttt ctggcccagg
      181 gtcttggggc gcaccggctt gccctttgcg gtcagggcga taaaatcccc cgtcagctct
      241 tttactctgc cctcgtcgcc ggaggccgcc agcgagatgc agtagtgcag cgcctgttcg
      301 tccagcggcg tcttggaccg ccacagctgc agcatggcgt cgatggcccg gcccgcgcca
      361 tctacggccg cctcctcgcc cgtcaccttg atctcggtgc cgcggcagac cgcgcacacg
      421 ccgaactcgc gctcgatctt ttttatattt tcatccccgc tgcccagtac cgccagcgcg
      481 tcgtcgatgg aatccagatt cagcagccgt tccgccatgc aggccctcct cgctttttaa
      541 ccataaaacc acgggattgt tttggcaaaa tagccaaatt tttgccgtgt ggatttatta
      601 tagcacattc cccgggcttc taacagggaa aattcgtgta aagttgttgc ttttttgtta
      661 ttttttgatt tttctgtgcc cgttttttct ctgcgcggcc cggcgtccgt ccctctgtcc
      721 cgccgcccgt gccccggggc gcttttgggg ccgtgggacg gaaaagtccg ccctgcgggc
      781 gggctatttg gcgatggcca gggcgatgtc ctccagccgc tccaccgtca gggtggccac
      841 gtactcctcc ccctgcatct cgcccgtata ggcggcggag atcatctgct cagtctgccc
      901 gtcctccgcc agcgcccgat ccaaccgaaa cttggcgcgg ttttttgccg tctcctcggt
      961 gagcaggatc tcgctctcct cctccggata gactgagcgc gtctcgagaa ccgccggcaa
     1021 agcgaagccg cacaccgtca gctgctcgta gcggacctcc tcggaataga gctgcggcat
     1081 ctgcgccata ctgcccagct cgagccgccg gtcgcccagc aagagggcgc ggtattccag
     1141 cacctccccc gtgggcacat gggcgacgaa gtgcagcggc tggtttaccg tatagatctt
     1201 ttccgtgcgg gcgatcaccc tggcctccgc cggggtgatc acgacgctgc ccgtcttgtc
     1261 gtcgatatag gtgcccgaga ccaacagctc cccctcgctc acccgctgcc cctgcagcac
     1321 cgccgcaaag ccgtgggcca cgtcgagatg gtcgatgatc ccggccttgg aggccacgat
     1381 atcccccggg gagtgctttt ccagctccgg ccgcgccgtg cgctcgctga cctccaccac
     1441 cagccgcccc tccacaaaat tcagcgagac gtcggccagg tcctccgact gcagcaggat
     1501 gatctggcgc acccggcgca gctgctcgtc ggtgggccgg acgccggcgt agacgccgtt
     1561 ggccgccagc gtggcgccga tctcctgctg ataccgctgc ggcacatcgt agtaccgcac
     1621 gcaccagagg gtgccgggca ggtaaaggca gagggcgacg aacaccgccg gcccaagcag
     1681 caggccccag cgcccccaat agcggcgcag caaaaaaggc agcccgcgtt ttttgctgca
     1741 ccgctattgg gggcgctggg gcc
//
LOCUS       DBMDYA010000622         1760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13417_length_1760_cov_1.322483, whole genome shotgun sequence.
ACCESSION   DBMDYA010000622 DBMDYA010000000
VERSION     DBMDYA010000622.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13417_length_1760_cov_1.322483"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="ACZPFK_12635"
     CDS             <1..354
                     /locus_tag="ACZPFK_12635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930269.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1407197.1"
                     /translation="YRELQSFAQFGSDLDMDTKTRLAQGERIVKVLGQGRGRPVPVEK
                     QVCIFYAVVHDYLKEVEVEAVPAFEEGLYDYLDSRAPAVLDAIRESGRLSEETEEQLK
                     EALKAYQAQFAALPR"
     gene            452..1291
                     /gene="atpG"
                     /locus_tag="ACZPFK_12640"
     CDS             452..1291
                     /gene="atpG"
                     /locus_tag="ACZPFK_12640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1407198.1"
                     /translation="MKDIKLRIKSVESTMQITKAMELVASSKLNKAKLRVERSRPYFT
                     LLHQTLAEIAEANTEFSSVYVQRREVKKRCFVLIAGDRGLAGGYNSNLFKLAAEKMQG
                     APCCALPVGKKAVEFCHARGVETLTEAFSLAEDVSVSDCFAMSRLLCEGFLAGEFDEL
                     AVCYTNFVSMLSQSPAALGMLPLSPKMRARGSAAPRQLVLYEPGSEAVYDAIVPGYLA
                     GMLWGALCESFASEVAARRMAMDAASKNAAEMIEDLNLRYNRARQGAITQEITEIVAG
                     AEE"
     gene            1378..>1760
                     /locus_tag="ACZPFK_12645"
     CDS             1378..>1760
                     /locus_tag="ACZPFK_12645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564620.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1407199.1"
                     /translation="MKRENIGRIVQVTGPVLDIKFEEGQLPALLNAVEVEADGRRLVA
                     EVAQQIGDNVARCIAMSSTDGLTRGLPVRDTGGPITVPVGDATLGRIFNLLGEPVDNL
                     PAPETQERWAIHREPPAYDEQQSTT"
BASE COUNT          328 a          528 c          642 g          262 t
ORIGIN      
        1 taccgcgagc tgcagtcctt tgcgcagttc ggctctgacc tggacatgga caccaagacg
       61 cggctggccc agggcgagcg catcgtcaag gtgctgggcc agggcagggg ccgcccggtg
      121 ccggtggaaa agcaggtctg catcttctac gcggtggtcc acgattacct caaagaggtg
      181 gaggtggagg ccgtgcccgc ctttgaggag gggctgtacg actatctgga ctcccgcgcc
      241 ccggcggtgc tggacgcgat ccgcgagagc ggcaggctgt ccgaggagac ggaggaacag
      301 ctcaaggagg cgctgaaagc ctatcaggcc cagttcgccg ccctgccgcg ctgagggcgg
      361 gccgccccgg ggcggggtga acccgcgccg gaggcgcaag accgacagct gagagagaaa
      421 gggggcaggg cccatgagcg gcggcgcttc gatgaaagac atcaaactgc gcatcaagag
      481 cgttgagagc acgatgcaga tcaccaaggc gatggagctg gtggcctcct ccaagctgaa
      541 caaggcaaag ctgcgggtcg agcgcagccg gccctatttc acgctgctgc atcagactct
      601 ggccgagatc gcggaggcca atacggaatt ttccagcgtg tatgtgcagc ggcgcgaggt
      661 gaaaaagcgc tgctttgtgc tcatcgccgg cgaccggggg ctggccgggg gctacaactc
      721 gaacctgttc aagctggcgg cggagaagat gcagggcgcg ccctgctgcg cgctgccggt
      781 gggcaaaaag gcggtggagt tttgccatgc ccgcggggtg gagaccctca ccgaggcgtt
      841 cagcctggct gaggacgtgt cggtcagcga ttgttttgcc atgtcgcggc tgctctgcga
      901 gggctttctc gccggcgagt tcgacgagct ggccgtctgc tacaccaact ttgtctcgat
      961 gctcagccag tcgccggcgg cgctgggcat gctgcccctc tcgcccaaga tgcgggcgcg
     1021 cggctcggcc gcgccgcgcc agctggtgct gtacgagccc ggcagcgagg cggtctacga
     1081 cgccatcgtg cccggctatc tggcgggcat gctctggggg gcgctctgcg agtcgttcgc
     1141 gtccgaggtg gcggcccggc gcatggcgat ggacgcggcc agcaaaaatg cggccgagat
     1201 gatcgaggac ctgaacctgc gctacaaccg ggcgcgccag ggcgcaatca cccaggagat
     1261 caccgagatt gtggcgggcg ccgaggaata agggccgcgg cgcaggagat aggattgtat
     1321 aaaagactgg taaaaataca ttttgtaaaa ccggtatcga cgaaaagagg gcggcggatg
     1381 aagcgagaga acatcggcag aatcgtgcag gtcaccgggc ctgtgctgga catcaaattt
     1441 gaagaggggc agctgccggc gctgctgaac gcggtggagg tggaggcgga cggccgccgg
     1501 ctggtggccg aggtggcgca gcagatcggg gacaacgtgg cccgctgcat cgccatgagc
     1561 tccaccgacg gccttacccg cggcctgccc gtgcgggaca ccggcggccc catcacagtg
     1621 ccggtggggg acgccacgct ggggcgcatc ttcaacctgc tgggcgagcc ggtggataac
     1681 ctgcccgccc ccgagacgca ggagcgctgg gccatccacc gcgagccgcc cgcctatgac
     1741 gagcagcaga gcaccaccga
//
LOCUS       DBMDYA010000623         1755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13468_length_1755_cov_2.149335, whole genome shotgun sequence.
ACCESSION   DBMDYA010000623 DBMDYA010000000
VERSION     DBMDYA010000623.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_1755_cov_2.149335"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..817)
                     /locus_tag="ACZPFK_12650"
     CDS             complement(8..817)
                     /locus_tag="ACZPFK_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407200.1"
                     /translation="MGYYRRYFVLYLSVALAAGFLWAAADLAWDLPLLFSFPLFVLVV
                     LLAAIFCNVAGVRRYQQMNLRLLQTGDTQAFLRELSACEKAANRNTRNTIFIAKAYAY
                     LLEGRYDAASALLTGLDLASCRQPRARQNDRCAYSVRCAGLAINCNQYQAAEQHLAAL
                     RREVAALRPTDALHVHYAKMLQDLSQMLAVRRGEAGGHAEYFRNQLEMAGDTVGRAGA
                     AYYLARALLLEEGAQEQALEYFDYAAAAVPGLAVGRDAALRAAALRGTLHG"
     gene            complement(1007..>1755)
                     /gene="hflX"
                     /locus_tag="ACZPFK_12655"
     CDS             complement(1007..>1755)
                     /gene="hflX"
                     /locus_tag="ACZPFK_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1407201.1"
                     /translation="DRRHLRRRIDLIGHKLDAIEQRRAQTRRSRSRNAVPVVALVGYT
                     NVGKSSLLNRICGAEVEARDQLFATLDPTARRAALPSGQNVVFIDTVGFVSRLPHSLV
                     EAFKSTLEEAKYADIVLRVADASDPLREEQLRVTAEVLAEIGAAENETAVVYNKCDRM
                     HLAAWDGVCVSAKTGEGLDELLALLDARLASRVAEVELLLPYDKLALLAPLRQGGSVQ
                     AEEYRDDGVYVKALADRRSLHLFEPYLLYQ"
BASE COUNT          288 a          590 c          603 g          274 t
ORIGIN      
        1 gaaaggctca gccgtgcagt gtcccgcgca gggctgcggc ccgcagggct gcgtcccgcc
       61 ccaccgccag cccgggcaca gcggccgcgg cgtagtcgaa gtattccagc gcctgttcct
      121 gcgcgccctc ctccagcagc agggcgcggg ccagatagta ggccgcgccc gcacggccca
      181 cggtgtcgcc cgccatctcc agttggttgc ggaagtactc ggcgtggccg cccgcctccc
      241 cgcggcgcac cgccagcatc tgcgagaggt cctgcagcat tttggcatag tgcacatgca
      301 gcgcgtcggt aggcctcagc gccgccacct cccgccgcag ggcggccagg tgctgctccg
      361 ccgcctggta ctggttgcag ttgatcgcca accccgcgca gcgcaccgaa taggcgcagc
      421 ggtcgttctg ccgggcgcgc ggctggcggc agctggcgag atcgaggccg gtcagcaggg
      481 cgctggccgc gtcatagcgc ccttccaaaa ggtaggcgta ggccttggcg atgaagattg
      541 tgttgcgggt gttgcggttg gccgctttct cgcaggcgga gagctcgcgc agaaaagcct
      601 gggtgtctcc ggtctgcagc agccgcaggt tcatctgctg atagcggcgc accccggcga
      661 cattgcagaa gatcgccgcc agaaggacga ccagcacaaa gaggggaaag gagaacagca
      721 gcggcaggtc ccaggccagg tcggccgcgg cccagagaaa gcccgcggcg agggcgacgg
      781 agagataaag gacaaaataa cggcggtaat accccatgaa aatgcctcct tgacgggaat
      841 ttgatcggca gagcagccgt tcggcggtga gagccgccgc cccggacgga aaaagcttga
      901 aaggcccgcg gtcagctgag aaagcgctct tcgatggttg cagcgcgcct ttgcctttaa
      961 cgagaatttt tcgccctttg acggcgccgg cgcaacgccg gggcggctat tgatacagca
     1021 gatacggctc gaacaggtgc aggctgcggc ggtcggcaag ggcttttaca tacaccccgt
     1081 cgtcccggta ctcctctgcc tgcacgctgc ccccctggcg cagcggggcc agcagggcca
     1141 gcttgtcgta gggcagcagc agctcgacct cggccacccg cgaggcgagg cgcgcgtcca
     1201 gcagggccag cagttcgtcc aggccctcgc cggtctttgc cgagacgcag accccatccc
     1261 aggcggccag gtgcatgcgg tcgcatttgt tgtagaccac ggcggtctcg ttttcggccg
     1321 cgccgatctc cgccagcacc tcggcggtca cccgcagctg ctcctcccgc agcgggtcgg
     1381 aggcgtcggc tacgcgcagc acgatgtcgg cgtatttggc ctcctccagc gtggatttga
     1441 aagcctccac caggctgtgg ggcaggcggc tgacaaagcc cacggtgtcg atgaagacca
     1501 cgttctgccc gctgggcagg gcggcccggc gggcggtggg gtccagggtg gcgaacagct
     1561 ggtcccgcgc ctccacctcg gccccgcaga tgcggttcaa aaggctggat ttgcccacgt
     1621 tggtgtagcc caccagggcc accaccggca ccgcgttgcg actgcggctg cgccgggtct
     1681 gggcgcgccg ctgttcgatc gcgtccagct tgtgcccgat caggtcgatg cgccgccgca
     1741 gatgacggcg gtcat
//
LOCUS       DBMDYA010000624         1754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13481_length_1754_cov_1.189353, whole genome shotgun sequence.
ACCESSION   DBMDYA010000624 DBMDYA010000000
VERSION     DBMDYA010000624.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13481_length_1754_cov_1.189353"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1754
                     /gene="dnaE"
                     /locus_tag="ACZPFK_12660"
     CDS             <1..>1754
                     /gene="dnaE"
                     /locus_tag="ACZPFK_12660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1407202.1"
                     /translation="DMALKIEGMPRHASTHAAGVVITREAANEYVPLSSNDGQIVTQF
                     TMTTIEELGLLKMDFLGLRTLTVLHDAEKMVRETIDPAFCLDEVPDDDPEVFEMLSTG
                     EAYGVFQFESNGMRQVLAQIKPTGIEDMIAVISLYRPGPMDSIPTYIHNRHDPSAVRY
                     KVPQLEHILGVTNGCIVYQEQVMQICRELAGFSYGQADLVRRAMSKKKHDVMEQERVH
                     FIDGCKEPGKECAGCVANGIPRQVANDIFDEMSSFASYAFNKSHAAAYAVVAYQTAYL
                     KCHYVKQFMAALLTSVLENTDKVIEYSAECQRLGIRVLPPDINKSGLGFTVDGEDIRF
                     GLLALKNVGRNLINAVIEKWREGPYTSMYDFCSKMYGREINRRAVESFIKSGSFDAFG
                     DSRRAMLESLEGMLKSIEGENRRNLEGQISLFGGTDEEEKQNRYDLPKTPEYPHNELL
                     RMEKEISGLYLSGHPLDQYRDKIGRFATATISSLSGEEAEQMDNRQVTLVCAVVRIKY
                     LTTRSDSMMAFVTVEDLTGTIELLVFPKVLTQCGPAIFENAVVAVRGRVSVKEEEGAK
                     LVADEVCAIDQYQPPRAGG"
BASE COUNT          353 a          550 c          555 g          296 t
ORIGIN      
        1 gacatggccc tcaagatcga gggcatgccg cgccatgcct ccacccacgc cgcgggcgtg
       61 gtcatcaccc gcgaggcggc aaacgagtat gtgcccctct ccagcaacga cggccagatt
      121 gtgacccagt tcaccatgac caccatcgag gagctgggcc tgttgaagat ggactttctc
      181 ggcctgcgca ccctgacggt gctgcacgac gccgagaaga tggtgcggga gaccatcgac
      241 ccggcctttt gtctggacga ggtgccggac gacgacccgg aggtctttga gatgctctcc
      301 accggcgagg cctacggggt gttccagttc gaatccaacg gcatgcgcca ggtgctggcc
      361 cagatcaagc ccaccggcat cgaggacatg atcgcggtta tctcgctcta ccgccccggc
      421 ccgatggatt cgatccccac ctatatccac aaccggcacg acccctcggc cgtgcgctac
      481 aaggtgccgc agctggagca cattttgggc gtcaccaacg gctgcatcgt ctaccaggag
      541 caggtcatgc agatctgccg cgagctggcg ggcttttctt acgggcaggc cgacctggtg
      601 cgccgggcca tgagcaaaaa gaagcacgac gtcatggagc aggagcgggt ccatttcatc
      661 gacggctgca aagagccggg caaggagtgc gcgggctgcg tggccaacgg catcccccgg
      721 caggtggcca acgacatctt tgacgagatg tccagctttg cttcctatgc tttcaacaaa
      781 tcccacgccg cagcctatgc ggtggtggcc tatcagacgg cctacctcaa atgccattat
      841 gtgaagcagt tcatggccgc cctgctcacc agcgtgctcg aaaataccga caaggtcatc
      901 gagtactcgg ccgagtgcca gcggctgggc atccgggttt tgccgccgga tatcaacaag
      961 agcggcctcg gcttcaccgt ggacggggag gatatccgct tcggcctgct ggccctcaaa
     1021 aacgtggggc gcaacctcat caacgccgtc atcgaaaagt ggcgagaggg cccctatacc
     1081 agcatgtatg atttctgcag caagatgtac ggccgcgaga tcaaccgccg ggcggtcgag
     1141 agctttatca agagcggctc attcgatgcc tttggggaca gccggcgcgc catgctggag
     1201 agcctggagg gcatgctgaa gagcatcgag ggcgagaacc gccgcaacct ggaggggcag
     1261 atcagcctgt tcggcggcac cgacgaggag gaaaagcaga accgttacga cctgccgaag
     1321 acccccgagt acccccacaa cgagctgctg cgcatggaaa aggagatcag cggcctgtat
     1381 ctgtccggcc acccgctgga ccagtaccgg gacaagatcg gccgctttgc caccgccacc
     1441 atctcctcgc tgtcgggcga ggaggccgag cagatggaca atcgccaggt gacgctggtg
     1501 tgcgccgtgg tgcgcatcaa gtatctgacc acccgctccg actccatgat ggcctttgtc
     1561 acggtggagg acctgaccgg caccatcgag ctgctggtct tcccgaaagt gctcacccag
     1621 tgcggcccgg ccatctttga aaatgcggtg gtggcggtgc ggggccgcgt ctcggtgaaa
     1681 gaggaggagg gggccaagct ggtggcggat gaggtctgcg ccatcgacca gtatcagccc
     1741 ccccgggcgg gcgg
//
LOCUS       DBMDYA010000625         1753 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13488_length_1753_cov_1.139225, whole genome shotgun sequence.
ACCESSION   DBMDYA010000625 DBMDYA010000000
VERSION     DBMDYA010000625.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13488_length_1753_cov_1.139225"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="ACZPFK_12665"
     CDS             complement(<1..478)
                     /locus_tag="ACZPFK_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1407203.1"
                     /translation="MTELKQKKLFTGRLSMPLLAVFCTLLWGSAFPFVKLGYAAFGIG
                     GDASVYTKLLFAGLRFGLAGVVVLAVGSIAARRPLGFRKSELGPILLVALVQTVLQYI
                     FFYVGLSNTTGTLGSILTSTSAFFAVLGAPLMHDFCVISLSDLLTPWEQIERRLALA"
     gene            complement(657..>1753)
                     /locus_tag="ACZPFK_12670"
     CDS             complement(657..>1753)
                     /locus_tag="ACZPFK_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1407204.1"
                     /translation="GKMSKSKGNVVDPYLLAERYSTDALRYFLLRDFPFGSDGNFSNE
                     LLIGRINMDLANDLGNLLSRTTAMADKYFGGALPAAQDEGPEDAELLAMLGALRDKYE
                     AQMEKFSFQSALAEVFSVIARANKYIDETAPWVLAKDEAKKPRLARVLYNLAETLRLC
                     AVLLTPFMPATCAEIFAQIGAGADVQTWDSAAAVGALPAAATLHKGKNLFPRIDAAKE
                     LAELEAQRAAQAAEAVVAAKPDAPAPEAAADPVEHEAEINYDAFSKVEMTVGQVVSCE
                     RMKESKKLLKLSVFDGERERTILSGIAKWYAPEDLTGKKVGIVANLAPRPMMGGKYVS
                     EGMILAADTDDGGAAVFFFPDGVRPGSRIR"
BASE COUNT          315 a          573 c          569 g          296 t
ORIGIN      
        1 ccgcaagggc cagccgacgc tcgatctgct cccacggcgt gagcagatcc gacagggaga
       61 tcacgcagaa atcatgcatc agcggcgcgc ccagcaccgc aaaaaaggcg gaggtggagg
      121 tgaggatgct gccgagagtg ccggtggtgt tggagagacc gacataaaag aagatgtatt
      181 gcagcacggt ctgcaccagc gccaccagca gaatgggccc cagctcgctt tttcgaaagc
      241 ccagggggcg gcgggcggcg atgctgccca ccgccagcac caccaccccg gccaggccga
      301 agcgcagccc cgcaaagagc agctttgtgt agacgctggc gtcgccgccg atgccgaacg
      361 ccgcgtaccc cagcttgaca aaggggaacg cgctgcccca gagcagggtg caaaagaccg
      421 ccagcagcgg cattgaaagc ctgccggtaa aaagtttttt ctgtttgagc tccgtcacga
      481 tacacacctc gtttgcttta aaaaaggttg ccctgcgggc aaccttttga tgggtgatcc
      541 tctgctctcc gcacggcgtc cgggccgcgc aggcctttca aaaagccatc ggaggggaaa
      601 gagggaaaaa acttgcagtg agagcgccgt gcccgcgcag gcgcggcaca gggggctcag
      661 cggatgcggc tgcccgggcg caccccgtcg gggaagaaga agaccgccgc gccgccgtcg
      721 tcggtgtcgg ccgccaggat catgccctcg gagacatatt tgccgcccat catcgggcgc
      781 ggggcgaggt tcgccacgat gcccaccttt ttgccggtca ggtcctcggg ggcataccat
      841 ttggcgatgc cggagaggat ggtgcgctcg cgttcgccgt caaagaccga cagcttgagc
      901 agctttttgc tctctttcat gcgctcgcag gacaccacct gccccaccgt catctccacc
      961 ttgctgaaag cgtcgtagtt gatctcggcc tcgtgctcca ccggatcggc cgccgcctcg
     1021 ggcgcgggcg cgtcgggctt tgcggcaaca acagcctccg ccgcctgcgc ggctctctgc
     1081 gcctccagct cagccagctc tttggccgcg tcgatgcgcg gaaacaggtt tttgcccttg
     1141 tgcagggtcg ccgctgcggg cagggcgccc acggcggcgg cgctgtccca ggtctgcacg
     1201 tccgcgcccg caccgatctg cgcaaaaatc tcggcgcagg tggcgggcat aaacggcgtc
     1261 agcagcaccg cgcacagccg cagcgtctcg gccaggttgt acagcacgcg ggccaggcgc
     1321 ggctttttgg cctcgtcctt tgccagcacc cagggcgcgg tctcgtcgat gtacttgttt
     1381 gcgcgggcga tgaccgagaa cacctcggcc agcgcggact ggaacgagaa cttttccatc
     1441 tgcgcctcgt acttgtcccg cagcgcgccc agcatggcca gcagctccgc gtcctcgggg
     1501 ccctcgtcct gggcggcagg cagcgcgccg ccgaagtatt tgtcggccat cgccgtggtg
     1561 cgggacagca gattgcccag gtcgttggca aggtccatgt tgatgcggcc gatcagcagc
     1621 tcgtttgaaa agttgccgtc cgagccgaac gggaagtccc gcagcaaaaa gtagcgcagc
     1681 gcgtcggtgc tgtagcgctc ggccagcagg taggggtcga ccacattgcc cttggatttg
     1741 gacatcttgc cgc
//
LOCUS       DBMDYA010000626         1752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13494_length_1752_cov_1.499091, whole genome shotgun sequence.
ACCESSION   DBMDYA010000626 DBMDYA010000000
VERSION     DBMDYA010000626.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13494_length_1752_cov_1.499091"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="ACZPFK_12675"
     CDS             <1..105
                     /locus_tag="ACZPFK_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1407205.1"
                     /translation="INGSVKNPHVIDQSKCIKCGACMEKCKFGAISKG"
     gene            124..>1752
                     /locus_tag="ACZPFK_12680"
     CDS             124..>1752
                     /locus_tag="ACZPFK_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HIR1407206.1"
                     /translation="METVNIKINNVPYTVKAGSTILEACHEVGIDIPTLCYLKEINEI
                     GACRICVVEVKGAKSLVTACVYPVNEGMEIFTNTPKVFAARKTNLELVLSTHNKDCLA
                     CVRNGSCELQALCLRYGVSNSERFAGEMPESEIDAMSPHLIRDNSKCVLCRRCVAVCK
                     SLQHVGVIGPNDRGFKTHIGSAFEANLVQTSCIHCGQCIVACPTGALREKADTDKVWA
                     ALADPSKHVVVQPAPSVRATLGECFGDPIGTNVQGKMVAALRRLGFDKVFDTDFAADL
                     TIMEEANEFIERVQNGGKLPLITSCSPGWVKFCEYYHPDFVENLSSCKSPQQMFGATV
                     KSYYAEKAGIDPKDIVCVSVMPCTAKKFEIKRNDQAANGVPDVDISITTRELSRMIDR
                     AGIQWADLPEEAFDPLMGESTGAGTIFGATGGVMEAALRTAVETLTGKPLENVEFHDV
                     RGTQGIKEATYDVAGMKVNVCVASGLENAHQVLRSIEKGEKSYHFIEFMACPGGCVNG
                     GGQPYQPASVRNFTDLKALRAKALYSEDESQTYRK"
BASE COUNT          363 a          574 c          545 g          270 t
ORIGIN      
        1 atcaacggca gcgtgaagaa cccgcatgtc atcgaccagt ccaagtgcat caagtgcggc
       61 gcctgcatgg agaagtgcaa gttcggcgcg atttcaaaag gttaagagag ggagtgaaga
      121 aacgtggaga ctgtaaacat caaaatcaac aacgtgcctt acaccgttaa ggccggttct
      181 accattctcg aggcctgcca tgaggtgggc atcgacatcc cgaccctttg ctacctcaag
      241 gagatcaacg agatcggcgc ctgccgcatc tgcgtggtcg aggtcaaggg cgccaaaagc
      301 ctggtcaccg cctgcgttta cccggtgaac gagggtatgg agatcttcac caacacgccg
      361 aaggtctttg ccgcgcgcaa gaccaacctg gagctggtgc tctccaccca caacaaggac
      421 tgcctggcct gcgtgcgcaa cggctcctgc gagctgcagg cgctctgcct gcgctacggc
      481 gtgagcaact ctgagcgctt tgcgggcgag atgcccgaga gcgagatcga cgccatgtcg
      541 ccgcacctga tccgcgacaa ctccaagtgc gtgctctgcc gccgctgcgt ggcggtctgc
      601 aagagcctgc agcacgtggg ggtcatcggc cccaacgacc gcggcttcaa gacccacatc
      661 ggctctgcgt tcgaggcgaa cctggtgcag accagctgca tccactgcgg ccagtgcatc
      721 gtggcctgcc ccaccggcgc gctgcgtgaa aaggccgaca ccgacaaggt gtgggcagcc
      781 ctggccgacc ccagcaagca cgtggtggtg cagcccgcgc ccagcgtgcg cgccaccctg
      841 ggcgagtgct tcggcgaccc catcggcacc aacgtgcagg gcaaaatggt ggccgcgctg
      901 cgccgcctgg gctttgacaa ggtgttcgac accgactttg ccgccgacct gaccatcatg
      961 gaggaggcga acgagttcat cgagcgcgtc cagaacggcg gcaagctgcc cctgatcacc
     1021 agctgcagcc ccggctgggt caagttctgc gagtactatc accccgactt tgtggagaac
     1081 ctgtccagct gcaaatcgcc gcagcagatg ttcggcgcca cggtcaagag ctattacgcc
     1141 gaaaaggccg gcatcgaccc gaaagacatc gtctgcgtgt cggtgatgcc ctgcacggcc
     1201 aaaaagtttg agatcaagcg caacgaccag gccgccaacg gcgtgcccga cgtggatatc
     1261 tcgatcacca cccgcgagct gtcccgcatg atcgaccgcg cgggcatcca gtgggccgac
     1321 ctgcccgagg aggcctttga cccgctgatg ggcgagtcca ccggcgcggg caccatcttc
     1381 ggcgccaccg gcggcgtgat ggaggcggcc ctgcgcaccg ccgtcgagac cctcaccggc
     1441 aagccgctgg agaacgtgga gttccacgac gtgcgcggca cccagggcat caaggaggcc
     1501 acctatgacg tggccggcat gaaagtcaac gtctgtgtgg ccagcggcct ggagaacgcg
     1561 caccaggtgc tgcgcagcat cgaaaaaggc gaaaagagct atcacttcat cgagttcatg
     1621 gcctgcccgg gcggctgcgt caacggcggc ggccagccct atcagccggc cagcgtgcgc
     1681 aacttcaccg acctgaaggc cctgcgcgcc aaggccctgt acagcgagga cgagagccag
     1741 acctatcgca ag
//
LOCUS       DBMDYA010000627         1745 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13567_length_1745_cov_1.381387, whole genome shotgun sequence.
ACCESSION   DBMDYA010000627 DBMDYA010000000
VERSION     DBMDYA010000627.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13567_length_1745_cov_1.381387"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="ACZPFK_12685"
     CDS             complement(<1..280)
                     /locus_tag="ACZPFK_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407207.1"
                     /translation="MRGITKIYPNGFVANKDVNLAVREGEIHALVGENGAGKSTLMKV
                     LFGVESHEEGEILLHGKPVNITDPISAIDMGIGMVHQHFMLVPSLTVAE"
     gene            complement(441..1598)
                     /locus_tag="ACZPFK_12690"
     CDS             complement(441..1598)
                     /locus_tag="ACZPFK_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1407208.1"
                     /translation="MKKLMALLMACALSMSLLAGCGGGGSSSTAGSSAAGGSQASSGA
                     DSKPIKVAMVIAGALGDKGFNDSAKAGLDKAMEEFGVEAQVIELPTSDKTKFEPTLLD
                     LADSGEYDLITAAGNAMREIVEKVAVQCPDQKFYLYDAAVDYNNGEFPNVYCNTFLQN
                     EASFLGGIVAAGMTSSDLPDMNADKVVGNVLMMDMAVINDFMVGFIEGCQYADPDVKI
                     NTAYIGAVDAAKAKDMAMAMYQQKADIVFQVAASAGLGVIEAGKEQNGYVIGVDQDQA
                     MVLMETDPEAANRILTSVLKRVDVAVYRVLEMMAKDPASVPWGKSEALGMQEGCVGIA
                     KTEVYEKLVTDEVKAMVEEAEKKIASGEIKVSTAYGMETDEITALRDGVKP"
BASE COUNT          275 a          582 c          513 g          375 t
ORIGIN      
        1 tttccgccac cgtgagcgag ggcaccagca tgaagtgctg atggaccatg ccgatgccca
       61 tatcgatggc gctgatggga tcggtgatgt tcaccggctt gccgtgcagc aggatctcgc
      121 cctcctcgtg gctctcgacc ccgaagagca ctttcatcag ggtgctcttg ccggcgccgt
      181 tctcccccac cagggcgtgg atctcgccct cgcgcaccgc caggttgaca tccttgttcg
      241 ccacaaagcc gttggggtag atcttggtga tgccgcgcat ctccagaatc acgttttccg
      301 acacccgaac tcctccttcc tcccggcggc cgccgccggg accatttctc cgtggccgcg
      361 ccgcgcggcc ccctcatcaa aaggacctcc cacccgcacc gcgggcggga ggttcccgat
      421 cttacgcctc tttttcaggg tcagggcttc acgccgtcgc gcaaagcggt gatctcatcg
      481 gtctccatgc cgtaggccgt gctgaccttg atctcgccgc tggcgatctt tttctcggcc
      541 tcctcgacca tggctttcac ctcgtcggtg accagctttt catagacctc ggtcttggcg
      601 atgccgacgc agccctcctg catgcccagc gcttcggatt tgccccaggg cacggaggcg
      661 gggtccttgg ccatcatctc gagcacgcgg tagacggcga cgtcgacgcg cttgagcacc
      721 gaggtgagga tgcggttggc ggcctcgggg tcggtctcca tcagcaccat ggcctgatcc
      781 tggtcaacgc cgatcacata gccgttctgc tccttgccgg cctcgatgac gcccaggccg
      841 gcgctggccg ccacctgaaa gacgatgtcc gctttctgct ggtacatggc catggccata
      901 tctttcgcct tggcggcgtc caccgcgccg atgtaggcgg tgttgatctt gacatccggg
      961 tcggcgtact ggcagccctc gataaagccc accataaagt cgttgatgac ggccatatcc
     1021 atcatcagca cgttgccgac gaccttgtcc gcgttcatgt ccggcaggtc ggagctggtc
     1081 atgcccgcgg ccacgatgcc gcccaggaag ctggcctcat tctgcaaaaa agtgttgcag
     1141 tagacgttgg ggaactcgcc gttgttgtag tccaccgcgg cgtcgtacag gtaaaatttc
     1201 tgatccgggc actgcaccgc cactttttcc acgatctcgc gcatggcgtt gccggcggcg
     1261 gtgatcaggt catactcgcc cgaatccgcc aggtccagca gggtgggctc aaacttggtc
     1321 ttgtcgctgg tgggcagctc gatgacctgg gcctcgacgc cgaattcttc catcgctttg
     1381 tccaggcccg ccttggcgct gtcgttaaag cccttgtcgc ccagggcgcc ggcgatgacc
     1441 atcgccacct tgatgggctt gctgtcggcg ccggacgacg cctgagagcc gcccgccgcg
     1501 gacgagcccg ctgtggatga agagcccccg ccgccgcagc cggccagcag gctcatggaa
     1561 agtgcgcatg ccatgagaag tgccattaac tttttcattt tttcctcctg atcaagcttt
     1621 ttctatgaag accgggcgtt ggcccggccg cgaacctgat gaaaagttcc cgcctgctag
     1681 agtcttctgg cggtcttgcc gaaagaagcg acctcgtccc aggtggggat gctgttggcg
     1741 gcgcc
//
LOCUS       DBMDYA010000628         1744 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13575_length_1744_cov_1.428484, whole genome shotgun sequence.
ACCESSION   DBMDYA010000628 DBMDYA010000000
VERSION     DBMDYA010000628.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13575_length_1744_cov_1.428484"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="ACZPFK_12695"
     CDS             complement(<1..177)
                     /locus_tag="ACZPFK_12695"
                     /inference="COORDINATES: protein motif:HMM:NF020476.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HIR1407209.1"
                     /translation="MKMILSVSRRTDIPAFYADWWYRRVEEGFVLVPSPYDPGRISRV
                     SLSPAAVDCIVFWSK"
     gene            619..1623
                     /locus_tag="ACZPFK_12700"
     CDS             619..1623
                     /locus_tag="ACZPFK_12700"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR1407210.1"
                     /translation="MDHFIVSGPPYEAGLRLGKELRQGGRGPGVVPALAMTQEKRLFA
                     RRCEEICAREFPEAVQELYGIADGLALPREELTGRIFCIYNFPPFQGCTCFAARSGGS
                     ALLGRNSDFMLELEGRCLSFLYRPATGFGFRGCATAPVQMEDGMNDCGLAVGLTFVWP
                     VTKKPGLHAGLLVRCLLQKCRTVEEGIAALKGLTISSSQTVTMADANGDMAVVECNAA
                     RVEVLRPCPGESFVGAVNDFRSAGMQPYAPGWEMMLSDQRYQTLRRALAARPDCDAAF
                     ARSLLAGEQGFLCQYDRALGADTLWSALYLPGLQKAFLCEGNPSRSPFREVVDGGENK
                     "
BASE COUNT          328 a          488 c          572 g          356 t
ORIGIN      
        1 cttgctccaa aagacgatgc agtccacagc ggcgggggag agggataccc tgctgatgcg
       61 cccggggtcg taagggcttg gtaccagaac gaagccctcc tccacccggc ggtaccacca
      121 gtcggcataa aaggcgggga tatcggtgcg gcggctgacg ctcaagatca tcttcatccc
      181 tcccgcggcg actgccgatt ctattctacc atatgggaca aaaaaatcga atgccttttt
      241 ggtgccagtt gaaaagattt tgcacgggcg cggggccgga gcagctgggc ccggcgggca
      301 aagtgggaca cggcgtcccc cggccctttt tgaaagagaa atgaaaaata gccgtcaggc
      361 tattttttcg gccatccggc cagtcctctg gccggggcca tcgagataaa tcgtttttta
      421 ttgcggagca ataaaaaaac aacttacagc ttattttaat agccccgttt tttgcccctg
      481 acgcggcgcc cgaaacggac agccccaaat agccgcatag cggctatttt tttatgcttg
      541 acataaaatt tttattccgc tatcctcggg gcatgggccg gcgccggctc tctgaaaaag
      601 aaaaaggaga gcgatcagat ggatcatttt atcgtgtccg ggccgccgta tgaggcgggg
      661 cttcggctgg ggaaagaact gcgccagggc ggccggggac cgggcgtcgt gccggccctt
      721 gccatgactc aggaaaaacg gctttttgcg cgccgctgcg aggagatctg tgcccgcgaa
      781 ttccccgagg cggtacagga gctgtacggc attgcggacg gcctggccct gccgcgggaa
      841 gagctgacgg gccgcatctt ctgcatctat aattttccgc cttttcaggg ttgcacctgt
      901 tttgcagccc gcagcggcgg cagcgccctg ttggggcgca acagcgattt tatgctggag
      961 ctggaggggc gctgcctcag ctttctctat cggcccgcca cgggcttcgg ctttcgcggg
     1021 tgcgccaccg cgccggtgca gatggaggac ggtatgaacg actgcgggct ggcggtgggc
     1081 ctgacctttg tctggcccgt cacgaagaag ccgggcctcc atgcggggct gttggtgcgc
     1141 tgtctattgc aaaaatgccg taccgtagag gaggggatcg cagcgctcaa aggcctgacc
     1201 atctcatcgt cacagaccgt caccatggcg gatgcaaacg gcgatatggc ggtggtggag
     1261 tgcaacgccg cgcgggtgga ggtgctgcgc ccatgcccgg gggagagctt tgtgggggcg
     1321 gtgaacgatt ttcgatccgc cgggatgcag ccctatgcgc ccgggtggga gatgatgctg
     1381 tccgatcagc ggtatcaaac cctgcgccgc gcgctggcgg cgcgcccgga ttgtgacgcg
     1441 gctttcgccc gcagtctgtt ggcgggcgag cagggctttt tgtgccagta cgaccgcgcc
     1501 ctgggggcgg atacgctctg gtcggcgctc tatctgccgg gtctgcaaaa ggcttttctc
     1561 tgcgagggga acccctcgcg cagccctttt cgggaggtag tcgacggagg ggagaacaaa
     1621 tagacccggg cgatttgttt gggccttccg tctcggccgc cgcgatggcg gcgaaaaacg
     1681 aggccattga aataagtcgt tttaccgtcc gccctgtcct gcatgcgggc tgaaaaaagg
     1741 atga
//
LOCUS       DBMDYA010000629         1737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13655_length_1737_cov_1.083741, whole genome shotgun sequence.
ACCESSION   DBMDYA010000629 DBMDYA010000000
VERSION     DBMDYA010000629.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13655_length_1737_cov_1.083741"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            379..1221
                     /locus_tag="ACZPFK_12705"
     CDS             379..1221
                     /locus_tag="ACZPFK_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1407211.1"
                     /translation="MPNVYKQSFKQTQTNNIELSIYNCGLQSCEKGYSWGPGVRDHYL
                     IHYVASGKGTYVVSGVTYEVKSGDVFLAKPSQLITYTADQEDPWEYYWVGFNGSNANR
                     LALQLPFVADTPVHTCKNPEKIKKALFNIFLSRGPENYSEALMVGYLYIFLAELMKEN
                     DRPESRAQSSSSAYVTSAIKFIQFNYSHEITIDDIAKAVGVSRSHLYRVFMSNIGESP
                     IDYLTNYRINEACYLLRCGNLSIAEIATSVGFFDQFYFSRVFKKSKGVPPSKYLASKE
                     ASQP"
     gene            1255..>1737
                     /locus_tag="ACZPFK_12710"
     CDS             1255..>1737
                     /locus_tag="ACZPFK_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407212.1"
                     /translation="MKKKFAALLAAFALFSALTGCTLPERLRILGAPKLAVLGASPQG
                     EYPDLPRQAILQAAGDVGLEAVPDDGGADSLASLALEQQATAFAVCLAQAQDAGAYLE
                     SARALGLPVIFCGERPSDEAMRSYDKCWYVGGSPAKQGELLGEALFEAYLCGMIPDKN
                     A"
BASE COUNT          347 a          562 c          493 g          335 t
ORIGIN      
        1 agctccacct cgcggccgac gccgtagagc gcttcaaagc gccgcacggc ggccacatag
       61 cgcgcccgct gacggcccac ccccgcctca tccgcaccgt aaagggcgcg gaacgcggcg
      121 tcataagcgc cgctctcgat gctgccgatc agctcttgtg tcttcatcat ttcaagctca
      181 cctcgttttg ctctgcttcg ccgacaggcc cccgcccgcc gcgcgcggcg aagcggcgca
      241 cggcggccct atttatcttt tattataaca aaccgcctgt gagccgtaaa ccgacttttt
      301 tgtgatagca catggacttt tgttgattct tctgatacaa taaattttgt atcaatcttg
      361 cgggaggagg aggctgtctt gccgaacgta tacaagcaga gcttcaagca gacccagacc
      421 aacaacatcg agctctctat ctacaactgc ggcctgcaga gctgtgaaaa ggggtactcc
      481 tgggggccgg gggtgcgcga ccactacctg atccactatg tggccagcgg caagggcacc
      541 tatgtggtga gcggcgtcac ctacgaggta aaaagcggcg acgtcttttt ggccaagccc
      601 tcgcagctga ttacttacac cgcggatcag gaggacccct gggagtacta ctgggtgggg
      661 ttcaacggct ccaacgccaa ccgtctggcg ctgcagctgc cctttgtggc ggacaccccg
      721 gtgcacacct gcaaaaaccc tgaaaagatc aaaaaagcgc tgttcaacat ctttctctcc
      781 cgcgggccgg aaaattacag cgaggccctg atggtgggat atctctatat ttttctcgcc
      841 gagctgatga aagaaaacga ccgcccggag agcagggccc agtccagcag cagcgcctat
      901 gtgaccagtg cgatcaaatt catccagttc aattactccc acgagatcac catcgacgac
      961 atcgccaagg cggtgggggt ctcgcgcagc catctctacc gggtttttat gagcaacatc
     1021 ggtgaatccc ccatcgacta tctgaccaac taccgcatca acgaggcctg ttacctgctc
     1081 aggtgcggca acctctccat cgcggaaatc gccacctccg tgggcttttt cgaccagttc
     1141 tatttttccc gcgtcttcaa aaagagcaag ggcgtgccgc ccagcaaata cctcgccagc
     1201 aaggaggcca gccagccgta ggcccgcccg gcgcccgacg gcttttgatc gcctatgaaa
     1261 aagaaatttg cggcgctgct tgccgctttt gcgcttttca gcgccctcac cggctgcacg
     1321 ctgcccgagc gcctgcgcat tttgggcgcg cccaagctgg ccgtgctggg cgcctcgccg
     1381 cagggcgaat accccgatct gccccggcag gccatcctgc aggccgcagg cgacgtcggc
     1441 ctggaagcgg tccccgacga cgggggtgcg gacagcctgg cctccctggc actggagcag
     1501 caggccaccg catttgccgt gtgccttgcc caggcgcagg acgccggggc ttatctcgag
     1561 agcgcccgcg ccctgggcct gccggtgatc ttctgcggcg agcggccctc agacgaggcg
     1621 atgcgcagct acgacaagtg ctggtatgtg ggcggcagcc cagccaagca gggcgagctg
     1681 ctgggagaag cgctttttga ggcctatctc tgtggcatga tccccgacaa aaacgcg
//
LOCUS       DBMDYA010000630         1734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13682_length_1734_cov_1.657685, whole genome shotgun sequence.
ACCESSION   DBMDYA010000630 DBMDYA010000000
VERSION     DBMDYA010000630.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13682_length_1734_cov_1.657685"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="ACZPFK_12715"
     CDS             <1..173
                     /locus_tag="ACZPFK_12715"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922033.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1407213.1"
                     /translation="GFIKTMWCGDAACEEKMKEEAGLSSRCMPFEQEHLSDVCPCCGK
                     PAKTMVIWGKAY"
     gene            complement(254..727)
                     /locus_tag="ACZPFK_12720"
     CDS             complement(254..727)
                     /locus_tag="ACZPFK_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HIR1407214.1"
                     /translation="MKKKVLLLCLLGAPIGLALSTLITIAISFSVGDGVFYPVVPELA
                     AQCGGELNAVLLQAVCSMLYGAAWAGASAVWNREGWSLLRQTLTHLLICSAATFPIAY
                     LMRWIPHSAAGALLYFGIFFGVYLAIWLLQYAAVKKKLRQINQRLGGDGIQPRQG"
     gene            complement(738..1178)
                     /locus_tag="ACZPFK_12725"
     CDS             complement(738..1178)
                     /locus_tag="ACZPFK_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR1407215.1"
                     /translation="MEIEVRIDSSRREPKVVIFTDRMTEEVDAIVKRLSGGQPQLLAG
                     FREGMLEILDPQTVLRVYAAAGKVFAATENGEYTLRPRLYELEETLGGFGFVRISNSE
                     IVNLKKVRGFDLSFAGTICVSLSDGSVTYVSRRYVSKIKQVLGI"
     gene            1523..>1734
                     /locus_tag="ACZPFK_12730"
     CDS             1523..>1734
                     /locus_tag="ACZPFK_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407216.1"
                     /translation="MDGNYVLEVINDYNAKIDELRKLIKSPKTFEPAMELALDIHAIT
                     HTGVVSSSTLPTFCDHLLEGLTEDDF"
BASE COUNT          369 a          496 c          521 g          348 t
ORIGIN      
        1 ccggctttat caagaccatg tggtgcggcg acgcagcctg cgaggagaag atgaaagagg
       61 aggccggcct ttccagccgc tgcatgcctt tcgagcagga gcacctctcc gacgtctgcc
      121 cctgctgcgg caagcccgcc aagacgatgg tgatctgggg caaggcctat tgagccttta
      181 cttcgtacag cctgcgtgaa aacaaaaaag gacggggcgc catggcgccc cgtccttttt
      241 tgttgttacg gcctcagccc tgacggggct gaatgccgtc cccgccgagc cgctggttga
      301 tctggcgcag cttctttttg acggccgcat attgcagcag ccaaatcgcc agatagacgc
      361 cgaagaagat gccgaagtag agcagcgccc ccgccgcgct gtggggaatc cagcgcatca
      421 gataagcgat ggggaaggtg gcggccgagc agatcagcag gtgggtgagg gtctggcgca
      481 gcaggctcca gccctcccgg ttccagaccg ccgaagcgcc cgcccaggcg gcgccgtaca
      541 gcatcgaaca cacggcctgc agcagcacgg cgttcagctc gccgccgcac tgcgccgcca
      601 gctcgggcac caccggataa aagaccccgt ccccgacgga aaaggagatc gcgattgtga
      661 tcagggtgct gagggccagg ccgatggggg ccccgagcag gcagagcagc aaaacctttt
      721 ttttcatctc gttcacctca tattcccaat acttgtttga tcttggagac gtagcgccgg
      781 gagacgtagg tcaccgagcc gtccgacagg gagacacaga tggtgccggc aaagctgagg
      841 tcgaacccgc gcaccttttt caggttgacg atctcggaat tggagatgcg cacaaaacca
      901 aagccgccca gcgtttcctc cagctcgtac agccgcgggc gcagcgtgta ttctccgttc
      961 tcggtggcgg caaagacctt gcccgcggcg gcgtagaccc gcagcaccgt ttgtgggtcc
     1021 aagatctcca gcatcccctc ccgaaagccc gccagcagct ggggctggcc gccggacagc
     1081 cttttgacga tcgcatccac ctcttcggtc atgcggtctg taaagatcac cacctttggc
     1141 tcccggcggg agctgtcgat cctgacctcg atctccattg cggatcgcct ccttgtgtta
     1201 tcagtataaa cgccccggcg catcttttcc atcgccgggg cgcaggtggt cgttttgagt
     1261 gtataggtgg ttgagccggg gctgcacacc gggccggttg gtttttcttg gggccgcggc
     1321 gctcggccgt cttttgccgt gtcccgtgca accccccaaa taaagtaaga gccccgggca
     1381 aggagaaagg gcttgcccgg gggcagaaaa ttggcatggg aaaagaagct gtttgacatt
     1441 tgtgtacatt gatattagaa tgaaaaaact taaggcggcc ctttagccct ttggataata
     1501 cgatagaatg ggagaaaaga ggatggatgg caattatgtt cttgaggtta taaacgatta
     1561 caacgccaaa atagatgaat tgcgcaaatt gataaaatca cccaaaacat ttgagccggc
     1621 gatggagctg gcgttggata tccatgcgat tacccatacg ggcgtggtct cttccagcac
     1681 gctccccacg ttttgtgatc atctgttgga ggggctgacg gaggatgatt ttag
//
LOCUS       DBMDYA010000631         1732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13696_length_1732_cov_1.648069, whole genome shotgun sequence.
ACCESSION   DBMDYA010000631 DBMDYA010000000
VERSION     DBMDYA010000631.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13696_length_1732_cov_1.648069"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="ACZPFK_12735"
     CDS             complement(<1..1685)
                     /locus_tag="ACZPFK_12735"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR1407217.1"
                     /translation="MKIQPKDKRALMAAALGRIDCDLAIKNTRYVNLFTGEVYPATVY
                     VHQGFVVHVEDKDLEAGLEKARRVVDAGGRYIVPGLIDAHVHIESSLMTPRVFAGAVV
                     PRGVTAVITDPHEIGNVLGEEAVVYMHDAGCGLPMRQFINIPSCVPSVPGLEEAGASF
                     DAAVIDRLAKLPNVIGLAEVMDFLGVVDGEERMMSIIETAEKNGLYLQGHLPAGFGRM
                     VSAYRIGGPATCHETSRPGEARDKLRAGMYVDARESSMCYNLPAIWEDVKEMPWRDRL
                     SLCTDDREADDILENGQLDLVLRNIIKLGMAPIEAIRAATLHTAEAAHLENMGAVAPG
                     YVADLLLVDDLEQFTVHSVYFAGEEVAREGKLLAAIEKKEFAVEHKNSVDVPDLTLED
                     FRLKAPAGCGDRVKVNVLTYADQTSLTRCVTEEVPVKDGYLDISGDGALCYAMVVNRF
                     GSGNRTLGLVRGFGIDQGADGSTVSHDCHNLTLVYKDAASGLAVYNELRSCGGGISAA
                     LGGSILHTLKLPVGGLMSAAPAPETAAQATAMKAALRKLGLQMVNPLLRIATLA"
BASE COUNT          260 a          582 c          582 g          308 t
ORIGIN      
        1 gccagggtgg cgatgcgcag cagcgggttc accatctgca gccccagctt gcgcagcgcc
       61 gctttcatgg cggtggcctg ggctgccgtc tcgggggcgg gggccgcgct catcaggccg
      121 cccaccggca gcttgagggt gtgcaggatg ctgccgccca gcgccgccga gatgccgccg
      181 ccgcagctgc gcagctcgtt gtagacggca aggccgctgg ctgcgtcctt gtagaccagg
      241 gtcaggttgt ggcagtcatg gctgacggtg ctgccgtccg cgccctggtc gatgccaaag
      301 ccgcgcacca ggccgagggt gcggttgccg ctgccgaagc ggttgaccac catggcatag
      361 cagagcgcgc cgtcgccgct gatgtccaga tagccgtcct ttaccggcac ctcctcggtg
      421 acgcagcggg tgagggaggt ctggtcggca taggtgagca cattgacttt cacccggtcg
      481 ccgcagccgg ccggggcctt gaggcggaag tcctccagcg tcaggtcggg cacatcgacg
      541 ctgtttttgt gctccaccgc aaactctttc ttttcgatgg cggccaacag cttgccctcc
      601 cgggcgacct cctcgccggc aaagtagacc gagtggacgg tgaactgctc cagatcgtcc
      661 accagcagca ggtccgccac atagccgggc gccaccgcgc ccatattctc aagatgcgcg
      721 gcctcggccg tgtgcagggt tgccgcccgg atggcctcga tgggggccat gcccagcttg
      781 atgatgttgc gcagcaccag gtccagctgg ccgttctcca ggatgtcgtc ggcctcccgg
      841 tcgtcggtgc acaggctcag gcggtcccgc cagggcatct ccttgacatc ctcccagatg
      901 gcgggcaggt tgtagcacat cgaggattcg cgcgcgtcca catacatgcc ggcgcgcagc
      961 ttgtccctcg cctcgcccgg acggctggtc tcgtggcagg tggccgggcc gccgatgcgg
     1021 taagcgctca ccatgcggcc gaagcccgcg ggcagatgcc cctgcaggta aaggccgttc
     1081 ttttccgcgg tctcgatgat ggacatcatg cgctcctcgc cgtccaccac gccgagaaaa
     1141 tccatcacct cggccaggcc gatgacgttc ggcagcttgg ccagacggtc gatcaccgcg
     1201 gcgtcaaagc ttgcgcccgc ctcctccagc cccggcaccg agggcacgca ggaggggatg
     1261 ttgataaact ggcgcatggg caggccgcag cccgcgtcgt gcatgtagac gacggcctcc
     1321 tcgcccagca cattgccgat ctcatgcggg tcggtgatga ccgcggtcac gccccggggc
     1381 accaccgccc cggcaaagac ccggggggtc atcagactgc tctcgatgtg cacgtgggcg
     1441 tcgatcaggc cgggcacgat ataccggccc ccggcgtcca ccacgcgccg ggctttttcc
     1501 agcccggcct ccaggtcctt gtcctccaca tgcaccacaa agccctggtg gacatagacg
     1561 gtggcggggt agacctcgcc ggtgaaaagg ttgacatatc gggtattctt gatggccagg
     1621 tcgcagtcga tgcggccgag cgcggcggcc atcagggcgc gtttgtcctt gggctggatc
     1681 ttcatggatc gtcctccttt gtttttgggt gggcgtcggg ggagggggcg cg
//
LOCUS       DBMDYA010000632         1727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13751_length_1727_cov_1.600861, whole genome shotgun sequence.
ACCESSION   DBMDYA010000632 DBMDYA010000000
VERSION     DBMDYA010000632.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13751_length_1727_cov_1.600861"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="ACZPFK_12740"
     CDS             <1..268
                     /locus_tag="ACZPFK_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR1407218.1"
                     /translation="GKMALIITDRGMEAAKAISDETDRGSTLVRSIGTFSGSERDLLI
                     CACSKSQIFKVRSAAHAVDDDALVMITEVDEVYGEGFKPPEVKK"
     gene            1174..1497
                     /locus_tag="ACZPFK_12745"
     CDS             1174..1497
                     /locus_tag="ACZPFK_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1407219.1"
                     /translation="MKMITAIVNKEDSNAVSSALTKAGFSVTKLSTTGGFLMAGNVTM
                     LIGVEDEKVDEVISIISEHSKQRKEIVPSTASYGIGVTTAFPLEVTVGGATVFVTNIE
                     RFEKL"
     gene            1497..>1727
                     /locus_tag="ACZPFK_12750"
     CDS             1497..>1727
                     /locus_tag="ACZPFK_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407220.1"
                     /translation="MLERLCGNEALKENLSAALAAGRLPHSVLLCGAGGLGKNFAARL
                     LAADYLYPAGGPGAEAVLADRSPECLVVRAEGA"
BASE COUNT          372 a          518 c          539 g          298 t
ORIGIN      
        1 cggcaaaatg gccctcatca tcaccgaccg gggcatggag gcggccaagg ccatcagcga
       61 cgagaccgac cgcggctcca cgctggtgcg gtccatcggc accttttcgg gcagcgagcg
      121 ggacctgctc atctgcgcct gcagcaagag ccagatcttt aaggtgcgct cggcggccca
      181 cgcggtggac gacgacgcgc tggtgatgat caccgaggtg gacgaggtct acggcgaggg
      241 cttcaaaccg ccggaggtaa aaaagtgaac ggcagaggcg cggcccccgg ggccgcgctt
      301 tttttgccgg cggctgtgaa gaaagccgaa cgccgccgag cggcgcgccc gcgcgccccg
      361 tggcccgcca tacccccggc aggtacatac cccagtgggc cgtcgtgccc cgacgggagg
      421 tcgtgcctcg gcgggctgcc gcgccccgtg gcctgccgta cctcaacggt ttgccatgtc
      481 cccgcagaca gccgcgcccc ggcgagctct gccccgcacc ggcgcgggga aacggcgcgc
      541 ccgcccacgc cggtgaaaaa gcggacgggg gttttccact ttccgccggc gcgggcgcct
      601 caaaggggtc acagggaacg gctttcccca aattccacag agttttcaac attcccgtgg
      661 aaaacggggt ctggttttcc ccaatacaac attcctctgc cgcccaaatg catataaaac
      721 tctcggtttc tgcatatttt ggttttaaat ggtaaaacaa aggaagaaat tggaactgag
      781 aattacgatt tgtatagagc tgaaaaaaac gaaatgttga aaactctgtt gaaaatgtta
      841 ataacagcgg ttttgcatcc tattttttcc acaagcggcg gtgaggaagt gacaacctcc
      901 aaaaacttta cgttgaatcg gccggaaaag gggcgaggtg atatacattc tcaaaaaggg
      961 ggaagggcag gcgggcagga cgacaaaaat ccataaatcc gtgggcagga gcagaggttt
     1021 tcccactctt tccagaagtt ttccaccaaa aaatggccgt cggggcgggc gagaagcgca
     1081 aaatggtgga aatccgtgcc ccggcgtggt aaaatgagac tatcccaaga cggggcaaag
     1141 cccctctctc atggcgcaag gaggccaaac actatgaaaa tgatcacggc gatcgtcaac
     1201 aaagaggatt cgaatgcggt ttcatccgcc ctcaccaagg ccggcttttc cgtcaccaag
     1261 ctctccacca ccggcggctt cctgatggcg ggcaacgtca ccatgctcat cggcgtggag
     1321 gacgaaaagg tggacgaggt catttcgatc atctccgagc actccaagca gcgcaaggag
     1381 atcgtgccct ccaccgcctc ttacggcatc ggggtcacca ccgcgttccc gctggaggtc
     1441 acggtgggcg gcgcgaccgt cttcgtcacc aatatcgagc ggttcgaaaa gctctgatgc
     1501 tggaaaggct ctgcggcaac gaggcgctga aagagaacct ctcggcggcg ctggccgccg
     1561 ggcggctgcc ccacagcgtt ttgctctgcg gcgccggggg gctgggcaaa aattttgccg
     1621 cgcggctgct ggcggcagat tacctctatc ccgcgggcgg gccgggcgcc gaggcggtgc
     1681 tggccgaccg ctcgccggag tgcctggtgg tgcgcgccga gggcgcg
//
LOCUS       DBMDYA010000633         1727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13753_length_1727_cov_1.420049, whole genome shotgun sequence.
ACCESSION   DBMDYA010000633 DBMDYA010000000
VERSION     DBMDYA010000633.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13753_length_1727_cov_1.420049"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            282..1145
                     /gene="fba"
                     /locus_tag="ACZPFK_12755"
     CDS             282..1145
                     /gene="fba"
                     /locus_tag="ACZPFK_12755"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1407221.1"
                     /translation="MLVNASEMLKKAVAGHYAVGQFNINNLEWTKSVLLTAEEMKAPV
                     ILGVSEGAGKYMTGFTTVAAMVAAMDKSLGITVPVALHLDHGTYEGAKACVAAGFTSI
                     MFDGSHYPIDENVAKTTELVALAHENGLSIEAEVGSIGGEEDGVVGMGEVADADECKR
                     IADLGIDFLAAGIGNIHGVYPANWKGLDFEALARIKAKIGDMPLVLHGGTGIPAEMIQ
                     TGISLGVAKINVNTECQLSFAAATRAYVEAGKDKEGKGFDPRKLLAPGAKAIQDTVRE
                     KIELFGSANKA"
     gene            complement(1531..>1727)
                     /locus_tag="ACZPFK_12760"
     CDS             complement(1531..>1727)
                     /locus_tag="ACZPFK_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407222.1"
                     /translation="LSRGGERVHTPTRESRGDCLTVAPPTAAGASPGSRRRRGGSLFF
                     YLYYPLHLAAIDLLRRRLYG"
BASE COUNT          331 a          524 c          596 g          276 t
ORIGIN      
        1 acggcgctgt tcgcggcgct gctggcgctg gtggtggtgc tggcgggcgt gccctgcctt
       61 tactcctatc tcgcctaccg cgccgaggag ggcgcggggc cgggcgggga aaaatagtgg
      121 aaaatttggc gcgcggggct ggaatttggg ccccgcgcct tgtttttgca gggcgtatcg
      181 gttataatga atctgttaca gaattaacaa gctgagccgg agagcggaca ggctgtaacg
      241 ggacgaaaat ccttttaatt taaagtatgg gggtaagcat catgctggta aatgcaagcg
      301 aaatgctgaa aaaagccgtg gccggccact atgcggtggg ccagttcaac atcaataacc
      361 tggagtggac caagtccgtc ctgctgactg ccgaggagat gaaagccccg gtcatcctgg
      421 gcgtatccga gggcgcgggc aaatacatga ccggctttac cactgtggcg gccatggtcg
      481 cggccatgga caaatccctg ggcatcaccg tgccggtggc gctgcacctg gaccacggca
      541 cctatgaggg cgcaaaggcc tgcgtcgccg ccggcttcac ctccatcatg ttcgacggca
      601 gccactatcc catcgacgag aacgtcgcca agaccaccga gctggtggcg ctggcccatg
      661 agaacggcct ttccatcgag gcggaggtcg gctccatcgg cggcgaggag gacggcgtgg
      721 tcggcatggg cgaggtcgcc gacgccgacg agtgcaagcg catcgccgac ctgggcatcg
      781 actttttggc cgccggcatc ggcaacatcc acggcgttta cccggccaac tggaaagggc
      841 tggattttga ggccctggcc cgcatcaaag cgaagatcgg cgacatgccg ctggtgctgc
      901 acggcggcac cggcatcccc gccgagatga tccagaccgg catctcgctg ggcgttgcca
      961 agatcaacgt caataccgag tgccagctct cgttcgccgc cgccacccgc gcctatgtgg
     1021 aagcgggcaa ggataaagag ggcaagggct ttgacccccg caagctgctc gcccccggcg
     1081 caaaggccat tcaggacacc gtgcgcgaga agatcgaact gttcggctct gcgaacaagg
     1141 cctgagctgc aaattaaaaa ccagagaggg acggccgcgg ccgtccctct ttttgtctgt
     1201 agcgcggttt gcagggggtc cggggcggag gttcgccggc cctgcgcccg cttgtgcggg
     1261 cgcagccgga tttgaaataa caaagggcgg cgggacgtgc agtatgtccc gccgcccttt
     1321 gcagcgtaaa aaagggagtg ccgcggcgag aggcgcccgg ccgcgaaagg ccccctcaaa
     1381 tccgccgcat cttgacccct ggccccgggg gagatgcgtt ctgccggggc gcaaaagggc
     1441 cgagacaggc gcgtgcgccc cggacgctgg gggccgcaca gcggggatgc gccccctgcc
     1501 gccggatgga aaacggcgcg tgcagagcgt tcagccatag agccggcggc gcagcagatc
     1561 aatggctgcc agatgcagcg ggtagtagag atagaaaaag agcgagccgc cccgacgcct
     1621 gcggctgccc ggcgaggccc ctgcggcggt gggcggggcg acggtaaggc agtcgcctcg
     1681 gctctcccgc gtcggtgtgt gcacccgttc gccgccgcgg gagagcc
//
LOCUS       DBMDYA010000634         1720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13827_length_1720_cov_1.495985, whole genome shotgun sequence.
ACCESSION   DBMDYA010000634 DBMDYA010000000
VERSION     DBMDYA010000634.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13827_length_1720_cov_1.495985"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="ACZPFK_12765"
     CDS             complement(<1..465)
                     /locus_tag="ACZPFK_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407223.1"
                     /translation="MSEALLEIKDLVVEYHTDEDDVHALNGITLSLDKGETLGLVGET
                     GAGKTTTALSIMRLLPKRIGEVKNGEIRFEGQNLLEKTEAEMRLIRGESISMIFQDPM
                     TSLNPVIRVGDQILEALKLHRPDMSRAVQAKKVDEMLEMVGIPAARKREYPHQ"
     gene            complement(477..1349)
                     /locus_tag="ACZPFK_12770"
     CDS             complement(477..1349)
                     /locus_tag="ACZPFK_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407224.1"
                     /translation="MKTKQIYKKRSQFSAVWRRLKKNKLAMLGLVILTAMILIAVCAD
                     LLADYDTAVIGQNMAERLQGPSASHWFGTDQFGRDVLARIIHGSRLSLSLSIIAMTVA
                     VAIGSCIGAVAGYYGGRVDDVLMRLMDILLAIPPMLMSISIVAALGQSMVNLLIALAI
                     AYIPVFARVIRSTILSIKGQEFVEAARACGTGNARIILRHIIPNAIGPIIVEATLTMG
                     AAILIISSLSFMGLGIQPPAPEWGTMLYEGRDVIRNDPYLVIFPGAAIALAVMSLNLL
                     GDGLRDALDPRLKN"
     gene            complement(1357..>1720)
                     /locus_tag="ACZPFK_12775"
     CDS             complement(1357..>1720)
                     /locus_tag="ACZPFK_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407225.1"
                     /translation="MTRSTMLEAIRQDYVRTARAKGAAERSVVLNHALRNALLPIVTV
                     IGVDFGYLLGGTVVIESVFSLPGLGTLLITAIRMKDTPVVMAAVMFVTIAYSLVNLLV
                     DIIYAFIDPRVKSQYAKG"
BASE COUNT          299 a          558 c          552 g          311 t
ORIGIN      
        1 ctggtgcggg tactcccgct tgcgggcggc ggggatgccc accatctcca gcatctcgtc
       61 cacctttttc gcctgcaccg cccgggacat atccggccgg tgcagcttga gggcctccag
      121 gatctggtcc cccacccgga tgaccgggtt gaggctggtc atggggtcct gaaagatcat
      181 ggaaatgctc tcgccccgga tcagccgcat ctcggcctcg gttttctcca gcaggttctg
      241 cccctcaaag cggatctccc cgtttttgac ctcaccgatg cgctttggca gcagcctcat
      301 gatcgagagg gcggtggtgg tcttgcccgc gccggtctcc cccaccagcc ccagcgtctc
      361 acccttgtcc aggctcagcg tgatgccgtt gagggcgtgt acgtcgtcct cgtcggtgtg
      421 atattccacc acgaggtctt tgatctccag caatgcttca ctcatggcgc acctccttaa
      481 ttcttcagcc gcgggtcgag ggcgtcgcgc agcccgtcgc ccagcaggtt gagggacatg
      541 acggccagcg cgatcgccgc ccccgggaag atcaccagat aggggtcgtt gcggatcacg
      601 tcccgcccct catagagcat ggtgccccac tcgggcgccg gcggctggat gcccaggccc
      661 ataaagctca gcgaggagat gatgaggatg gccgcgccca tggtcagggt cgcctcgacg
      721 atgatcggcc cgatggcgtt ggggatgatg tgccgcagga tgatgcgggc gttgccggtg
      781 ccgcaggccc gggccgcctc gacaaactcc tgccccttga tggagaggat ggtggagcgg
      841 atgacccggg cgaagaccgg tatataggcg atggccaggg cgatgagcag gttgaccatg
      901 ctctgcccca gcgccgccac gatggagatg gacatcagca tcggcgggat ggccagcaaa
      961 atatccatca gccgcatcag cacgtcgtcc acccggccgc cgtaatagcc cgccaccgcg
     1021 ccgatgcagg agccgatggc caccgccacc gtcatggcga tgatgctcag cgacagggac
     1081 aggcggctgc cgtggatgat gcgggccagc acgtcccggc caaactggtc ggtgccgaac
     1141 cagtggctgg ccgaggggcc ctgcagccgc tcggccatgt tctggccgat gaccgccgtg
     1201 tcgtagtcgg ccagcaggtc ggcgcagacc gcgatcagga tcatggcggt gaggatcacc
     1261 agccccagca tggccagctt gttctttttc agccggcgcc acacggcgga aaactggctg
     1321 cgctttttat agatctgttt cgtcttcacc gtgtcctcac ccctttgcat actgtgattt
     1381 gacccgcggg tcgataaagg cgtaaatgat gtccaccagc aggttcacca ggctgtaggc
     1441 gatggtcacg aacatgaccg ccgccatcac caccggggta tctttcatgc ggatggcggt
     1501 gatcagcagg gtgccgaggc ccggcaaaga aaagaccgac tcgatgacca ccgtgccgcc
     1561 cagcagatag ccgaagtcca cgccgatgac ggtgacgatg ggcaacaggg cgttgcgcag
     1621 cgcgtggttg agcaccaccg accgctccgc cgcgcccttg gcccgcgcgg tgcgcacata
     1681 gtcctgccgg atggcctcga gcatggtgga gcgggtcatg
//
LOCUS       DBMDYA010000635         1720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13828_length_1720_cov_1.226065, whole genome shotgun sequence.
ACCESSION   DBMDYA010000635 DBMDYA010000000
VERSION     DBMDYA010000635.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13828_length_1720_cov_1.226065"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="hisG"
                     /locus_tag="ACZPFK_12780"
     CDS             <1..514
                     /gene="hisG"
                     /locus_tag="ACZPFK_12780"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR1407226.1"
                     /translation="FENPAVGVRYLLVKPGDVTIYVEHGAADVGIVGKDILEDARPDV
                     YELLDLGIGRCRMAVAAKRGYVEDTGRTLRVATKFENIAKRYYAGLNRDIEVIRLGGS
                     IELAPLLGLSDVIVDIVESGNTIRENNLAVLTEFMEISARFIANKSSFKFKNSVITVM
                     TEKLSGVIGE"
     gene            511..>1720
                     /gene="hisD"
                     /locus_tag="ACZPFK_12785"
     CDS             511..>1720
                     /gene="hisD"
                     /locus_tag="ACZPFK_12785"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1407227.1"
                     /translation="MIKIMVAKEVAPEEVLNRDIRGEADVSAAVDEILENVRSRGDAA
                     LLEYARRFDGAALESLEVTKEELQAGWEAVDEGFRQTLTLARQNIAAFHEKQLHQNFV
                     MAEQPGVVLGQRYTPIERVGVYVPGGTASYPSTVLMDVVPAKLAGVGQIVMATPPAAD
                     GSVAPAILAAAKIAGVDRIFKIGGAGAVAALAYGTQSVPRVDKIVGPGNIFVATAKRK
                     VFGLVDIDMIAGPSEILVVADGGCDPAWVAADLLSQAEHDKLASAVLVTDSRPLAKAV
                     AAELERQAAQLPRREIAEASLAANGKIILTDSIAEAVEIANAIAPEHLELAVEDPFAL
                     LPGVKNAGSIFLGRCVPEALGDYFAGPNHTLPTSGTARFSSPLGVDDFVKRSSFLYYR
                     REALGKVADRV"
BASE COUNT          308 a          486 c          657 g          269 t
ORIGIN      
        1 gtttgaaaac ccggccgtgg gggtgcgcta tttgctggtg aagcccgggg acgtgaccat
       61 ctatgtggag cacggggccg cggacgtggg catcgtgggc aaggatatcc tggaggatgc
      121 gcgccccgac gtgtacgagc tgctggacct gggcatcggc agatgccgca tggcggtggc
      181 ggcaaaacgc ggctatgtgg aggacaccgg ccgcaccctg cgggtggcga ccaaatttga
      241 aaacatcgcc aagcggtact acgcggggct caaccgggat atcgaggtta tccgcttggg
      301 cggcagcatc gagctggcgc cgctgctggg cctctccgac gtgatcgtgg atatcgtgga
      361 gagcggcaac accatccgcg agaacaatct ggcggtgctc accgagttca tggagatcag
      421 cgcgcggttc atcgccaaca agtcgagttt caaattcaaa aacagcgtca tcaccgtgat
      481 gacggaaaaa ctgagcgggg tgatcggcga atgataaaga tcatggtggc aaaagaggtg
      541 gcgcccgagg aggtgctcaa ccgggatatc cgcggggagg cggacgtctc ggccgcggtg
      601 gatgagatac tggaaaatgt gcgcagccgc ggcgacgcgg cgctgctgga gtacgcgcgg
      661 cggttcgacg gggcggcgct ggaatcgctg gaggtgacaa aggaggaact gcaggccggc
      721 tgggaggcgg tggacgaggg gttccggcag acgctgaccc tggcgcggca gaacatcgcc
      781 gcctttcacg aaaagcagct gcaccagaat tttgtgatgg ccgagcagcc cggggtggtg
      841 ctggggcagc ggtatacgcc catcgagcgg gtgggcgttt atgtgcccgg cggcaccgcc
      901 agctacccca gcaccgtgct gatggacgtg gtgccggcaa agctcgccgg ggtggggcag
      961 atcgtgatgg cgaccccgcc cgcggcggac ggcagcgtgg cgccggccat cctggccgcg
     1021 gcgaagatcg cgggggtgga ccgcatcttc aagatcgggg gcgcgggcgc ggtggcggcg
     1081 ctggcctacg gcacccagag cgtgccccgg gtggacaaga tcgtggggcc gggcaacatc
     1141 ttcgtggcca cggccaagcg caaggtcttt gggctggtgg atatcgacat gatcgccggg
     1201 cccagcgaga ttttggtggt ggcggacggc ggctgcgacc ccgcatgggt ggccgcggac
     1261 ctgctgagcc aggcggagca tgacaagctg gcctccgcgg tgctggtgac cgacagcagg
     1321 ccgctggcaa aggcggtggc ggcagagctg gagcggcagg cggcgcagct gccgcggcgg
     1381 gagatcgccg aggccagcct cgccgccaac ggcaagatca tcctcaccga ttccatcgcc
     1441 gaggcggtgg agatcgccaa cgccatcgcg ccggagcatc tggagctggc ggtggaggac
     1501 ccctttgcgc tgttgcccgg cgtgaagaac gcgggcagca tctttttggg ccgctgtgtg
     1561 cccgaggcgc tgggcgatta ttttgccggc ccgaaccaca cgctgcccac cagcggcacg
     1621 gcgcgttttt ccagccccct gggggtggac gattttgtca agcgcagcag ctttttgtat
     1681 tacaggaggg aggcgctggg caaggtggcg gaccgggtgg
//
LOCUS       DBMDYA010000636         1716 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13868_length_1716_cov_1.379567, whole genome shotgun sequence.
ACCESSION   DBMDYA010000636 DBMDYA010000000
VERSION     DBMDYA010000636.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13868_length_1716_cov_1.379567"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            415..861
                     /locus_tag="ACZPFK_12790"
     CDS             415..861
                     /locus_tag="ACZPFK_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HIR1407228.1"
                     /translation="MPRPKKCRRVCALPREAGFSPLGRGDGGPPVEMAVDEYEAVRLI
                     DLLGCTQEECAAQMGVARTTVQAVYDAARRKLAEALVRGRGLEVRGGEFILCPRAEEC
                     CGRGCAGGGCARRRCAEAEQAAAPAAAKGGKKQEKGCCHHENCSHT"
     gene            839..1279
                     /locus_tag="ACZPFK_12795"
     CDS             839..1279
                     /locus_tag="ACZPFK_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1407229.1"
                     /translation="MKIAVTHENGNVFGHFGHTAEFKIYEVQDGKIVGAQVMPTGGSG
                     HGALAGFLQAQGVDALICGGIGGGARDALAAAGIALYPGVTGNADAAAAALAAGSLSY
                     DPAAKCAGHEHGHEGGCHGGEGGCGGHHGEGGCGEDKHGCSGRG"
     gene            1295..>1716
                     /locus_tag="ACZPFK_12800"
     CDS             1295..>1716
                     /locus_tag="ACZPFK_12800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1407230.1"
                     /translation="MKVLIIGGVAGGATAAARLRRLDEEAEIVILERSGYISYANCGL
                     PYYVGGEITDRKKLTLQTPESFGSRFRVEARVRQEALSIDRAAKQVRVRRLDDGSEYS
                     ESYDKLILAPGARPVRPPLPGIDGGRIFALRTVEDTFAL"
BASE COUNT          300 a          518 c          653 g          245 t
ORIGIN      
        1 caaaagcgct gcccctggag caaaattctt cgccattggg gttcgccttt ttctgcaagg
       61 gcccacgcag tgaccgccac tgacagggag tcaacgccgg ggtcatggca aaaaacaggg
      121 cgatcaaaac cagatgcttt ttacgcgcgt cccccccccc gctctggggg cgggcgtttg
      181 aaattttaag gcgatggccg gggcagcctg cggaagcgct gttatggctt ttttatggct
      241 ttgcgtcgta aaggtgtaaa agcgcctttg cgccgcgggc ggccggggat gaaaacatgc
      301 tcccccgctg caaagtaaag tgaagcagag cgccgggaaa acaatagtaa ttgacatatg
      361 tcaaaaacag tgctaggata gggtcatagg ccacagcgag aggaggcgtc cgggatgccg
      421 aggccaaaaa agtgccgccg ggtctgcgcc ctgccgcggg aagccggctt ttcccctctg
      481 ggccggggcg acggcgggcc gccggtggaa atggcggtgg acgagtacga ggcggtgcgc
      541 ctcatcgacc tgctgggctg tacccaggag gagtgcgcgg cccagatggg ggtcgcgcgc
      601 accacggtgc aggcggtgta cgacgcggcg cggcgcaagc tggccgaggc gctggtgcgc
      661 gggcgcgggc tcgaggtgcg gggcggcgag ttcatcctct gcccccgcgc ggaagagtgc
      721 tgcggccgcg gctgcgccgg cggcgggtgc gcgcggcggc gctgcgccga ggcggagcaa
      781 gcggccgccc ccgctgcggc aaagggcggc aaaaaacaag agaaaggttg ttgtcatcat
      841 gaaaattgca gtcacacatg aaaacgggaa cgtcttcggc cacttcggcc acacggcgga
      901 gttcaagatc tatgaggtgc aggacggcaa gatcgtcggc gcccaggtga tgcccacggg
      961 gggcagcgga cacggggccc tggccggctt tttgcaggcc cagggggtgg atgcgctgat
     1021 ctgcggcggc atcggcggcg gtgcgcggga cgccttggcg gccgcgggca tcgccctcta
     1081 cccgggggtc accggcaacg ccgacgcggc cgccgccgcg ctggctgcgg gcagcctgtc
     1141 ctatgacccg gcggccaagt gcgccgggca tgagcacggg cacgagggcg gctgtcacgg
     1201 tggcgagggc ggctgcggcg gccaccacgg cgagggcggc tgcggcgagg acaagcacgg
     1261 ctgcagcggc cggggctgag agggaggtgg ccgaatgaaa gtactcatca tcggcggcgt
     1321 cgcgggcggc gccacggcgg ccgcgcggct gcgcagactg gacgaggagg ccgagatcgt
     1381 gatcctggag cgcagcggct atatctctta cgccaactgc gggctgccct attatgtggg
     1441 cggcgagatc accgaccgca aaaagctgac cctgcagacg ccggagagct tcggcagccg
     1501 tttccgggtc gaggcgcggg tgcgccagga ggcgctctcc atcgaccggg cggccaagca
     1561 ggtgcgggtg cgcaggctgg acgacggctc agagtacagc gagagctacg acaagctgat
     1621 cctcgccccc ggcgcgcggc cggtgcggcc gccgctgccc ggcatcgacg gcgggcgcat
     1681 ctttgcgctg cgcacggtgg aggacacctt tgccct
//
LOCUS       DBMDYA010000637         1710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13925_length_1710_cov_1.229335, whole genome shotgun sequence.
ACCESSION   DBMDYA010000637 DBMDYA010000000
VERSION     DBMDYA010000637.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13925_length_1710_cov_1.229335"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>336
                     /locus_tag="ACZPFK_12805"
     CDS             <1..>336
                     /locus_tag="ACZPFK_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1407231.1"
                     /translation="SILYGLVGMMFFCQLLRLKTSLLAGALTLVVMILPTIVRTTQES
                     LKTVPESYREGALGLGATKWHMIRTIILPSSVDGIVTGCILAIGRIVGESAALLFTAG
                     LGLKLLGNL"
     assembly_gap    337..346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <347..518
                     /locus_tag="ACZPFK_12810"
     CDS             <347..518
                     /locus_tag="ACZPFK_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1407232.1"
                     /translation="EAMSGSGATLSVALYIYAMERGKFDVAFAVAAVLMLIVLVINFA
                     AKGLGKRLKNKI"
     gene            532..1287
                     /gene="pstB"
                     /locus_tag="ACZPFK_12815"
     CDS             532..1287
                     /gene="pstB"
                     /locus_tag="ACZPFK_12815"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1407233.1"
                     /translation="MENIITSKDLKLYYGNFEALKGITVEIAEREITALIGPSGCGKS
                     TFLKTLNRMNDLVQGVRVEGQVLYHGQDIFAPGVDVTQLRKNVGMVFQKPNPFPMSIY
                     DNIAYGPRLHGTHSKAELNDLVEQSLRGASLWEEVRDRLKKSALGLSGGQQQRLCIAR
                     ALAVQPDVLLMDEPTSALDPASTIRIEELMAELKKNYTVVIVTHNMQQAARISDKCAF
                     FLLGEMVEIGPTEQIFSMPSDKRTEDYITGRFG"
     gene            1514..>1710
                     /locus_tag="ACZPFK_12820"
     CDS             1514..>1710
                     /locus_tag="ACZPFK_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HIR1407234.1"
                     /translation="MSVRKTFDAELAELSASLIRMGSEAAGAIDNAIRAFDGHDLQLA
                     DAVIQGDRRLDDMEREIERHCL"
BASE COUNT          331 a          522 c          547 g          300 t
ORIGIN      
        1 cctccatcct ctacgggctg gtgggcatga tgttcttctg ccagctgctg cggctcaaga
       61 cgtcgctgct ggcgggcgcg ctgaccctgg tggtcatgat cctgcccacc atcgtgcgca
      121 ccacccagga gagcctgaag accgtgcccg agagctaccg cgagggcgcg ctggggctgg
      181 gggccaccaa gtggcatatg atccgcacca tcatcctgcc cagcagcgtc gacggcatcg
      241 tcaccggctg catcctggcc atcgggcgca tcgtgggcga gtcggccgcg ctgcttttca
      301 ccgccgggct ggggctcaag ctgctgggca atctctnnnn nnnnnncgag gccatgagcg
      361 ggtccggcgc gaccctcagc gtggcgctgt acatctacgc catggagcgc ggcaagtttg
      421 acgtggcctt tgcggtggcc gcggtgctca tgctgatcgt gctcgtcatc aattttgccg
      481 caaagggatt gggtaaacgg ctgaaaaaca agatataggg ggagaacccg cgtggaaaac
      541 atcatcacca gcaaggacct gaagctgtat tatgggaatt ttgaggccct gaaaggcatc
      601 acggtggaga tcgccgagcg ggagatcacg gccctgatcg gcccctcggg ctgcggcaaa
      661 tccacctttt taaagacgct caaccgcatg aacgacctgg tgcagggggt gcgcgtggag
      721 gggcaggtgc tctatcacgg gcaggacatc ttcgcccccg gcgtggacgt cacccagctg
      781 cgcaaaaatg tgggcatggt gtttcaaaag ccaaacccgt tccccatgag catctacgac
      841 aacatcgcct atggcccgcg gctgcacggc acccactcaa aggcggagct gaacgatctg
      901 gtggagcaga gcctgcgcgg cgccagcctg tgggaggagg tgcgcgaccg gctcaaaaaa
      961 tcggccctgg gcctttcggg cgggcagcag cagcggctgt gcatcgcccg cgccctggcg
     1021 gtgcagccgg atgtgctgct gatggatgag cccaccagcg cgctggaccc ggcctccacc
     1081 atccgcatcg aagagctgat ggccgagctg aagaagaact acacggtggt catcgtcacc
     1141 cacaatatgc agcaggcggc ccgcatcagc gacaagtgcg ccttttttct gctgggcgag
     1201 atggtggaga tcggccccac cgagcagatc ttcagcatgc cgtcggataa gcgcaccgag
     1261 gactatatta ccggccgttt cggctaaagc gcctgcccgg gcgccgcggg acgcggccaa
     1321 agggcggtgc ggagcgcgcc gccgcaaaag cacggccttt ttcacaaaaa atagccgtca
     1381 ggctattttc ggccatgtgg cccgtccctg ggccggggcc atcgagataa gtcgtttttt
     1441 attgcgcagc aataaaaaat ccttttcccg ccgcatcacg gcgggtataa tgaaagcgag
     1501 cgtgagatcg agcatgtctg ttcgaaaaac ttttgacgcc gagctggcgg agctgtccgc
     1561 cagcctgatc cgcatggggt cggaggccgc cggggccatc gacaacgcca tccgcgcctt
     1621 tgacggccac gacctgcagc tcgccgatgc ggtgatccag ggcgaccgcc gcctggacga
     1681 tatggagcgt gagatcgagc gccactgtct
//
LOCUS       DBMDYA010000638         1709 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13935_length_1709_cov_1.562811, whole genome shotgun sequence.
ACCESSION   DBMDYA010000638 DBMDYA010000000
VERSION     DBMDYA010000638.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13935_length_1709_cov_1.562811"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="ACZPFK_12825"
     CDS             <1..619
                     /locus_tag="ACZPFK_12825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1407235.1"
                     /translation="RKREKLAKEQLKKMGLGKRAHHKPTEMSGGQQQRVGIARAFVSR
                     PAVIFADEPTGNLDSHTTVQVMNAILKLIRENNITFVMVTHERDLAACADRIITLRDG
                     LVLRDEKMEHPQQAYVAPEGQEDADLDDNSWEDGTGKLASPPPLRQEEKKEEKTAEAT
                     PPLPGQAQRDETLAQISETIRRAKEKSPAAPSQTNEKTKENSTHE"
     gene            612..>1709
                     /locus_tag="ACZPFK_12830"
     CDS             612..>1709
                     /locus_tag="ACZPFK_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 family protein"
                     /protein_id="HIR1407236.1"
                     /translation="MSRKKTAFQKVLCSALSLLLMAGVLAAVPAGATEGTGGGSSGSG
                     QPTPEPTATQKPDIDQGGSPVITAYTVQDASGKELQRIEEGQKCQIVVAIADSRFTTL
                     AGLQTDGKLTADFANAKITSTGSFATPSLGDIRFTTPKDDGGVVRYAVVFNDITYLGG
                     ENKLSFDLSYKNQKVSLVNLTQAISQCVGASSTEGGKSSALVVKSASYGASEVQAGKE
                     FDLTAEILATAGTNAIDMVQVSLELPEKITVVSGNNNYYVGKLTAEASTAVTFRLLAS
                     AVTEPGSYNISVNVSGIGNDGTPVNNSLKVTVPVVQPERFEITNMEAYSPLFTGEEGT
                     VSVTYVNKGKGIIYNLSAKIEGEGMDNEGQNQ"
BASE COUNT          400 a          530 c          531 g          248 t
ORIGIN      
        1 acgaaagcgg gaaaaactgg caaaggaaca gctcaaaaag atggggctgg gcaagcgggc
       61 gcatcacaag cccaccgaga tgtcgggcgg ccagcagcag cgggtgggca tcgcccgcgc
      121 gtttgtcagc aggccggcgg tcatctttgc cgacgagccc accggcaacc tggattctca
      181 caccaccgta caggtgatga acgccatcct caagctgatc cgcgaaaaca atatcacctt
      241 tgtcatggtc acccatgagc gcgacctggc ggcctgcgcg gaccgcatca tcaccctgcg
      301 ggacggtttg gtgctgcggg acgagaagat ggagcatccc cagcaggctt atgtcgcccc
      361 cgaggggcag gaggatgcgg acctagacga caacagctgg gaggacggta ccggcaaatt
      421 ggcatcgccg cccccgctgc ggcaagaaga gaaaaaagag gaaaaaaccg cagaggcaac
      481 gcccccgctg cccgggcagg cgcagcggga cgagaccctg gcccagatca gtgagaccat
      541 ccgcagggca aaggagaaaa gcccggcggc cccgtcacag acgaacgaaa agacaaagga
      601 gaacagcacc catgagtaga aagaaaactg cgtttcaaaa agttctgtgc agcgccctgt
      661 cgctgctgct gatggcgggc gtcctggcgg ccgtccccgc gggggcgacg gagggcacgg
      721 ggggcggatc ctccggctct ggccagccca caccggaacc taccgccacc caaaagcccg
      781 acatcgacca gggtgggtcc ccggtcatca ccgcttatac ggtgcaggat gcgtcgggta
      841 aggagctgca gcgcatcgag gaggggcaga aatgccagat cgtcgtggcg attgcggatt
      901 cccgctttac cacccttgcg ggcttgcaga ccgacggtaa gctgaccgcc gactttgcca
      961 acgcaaagat cacctccaca ggttcttttg ccacgccaag cctgggcgat atccgcttca
     1021 ccacgcccaa ggatgacggc ggcgtagtgc gctacgcggt cgtcttcaac gacattacat
     1081 atctcggcgg ggaaaacaaa ctgagctttg acctgagcta caaaaaccag aaggtttcgc
     1141 tggtcaacct gacccaggcc atcagccagt gcgtgggcgc gtcctccacc gaggggggca
     1201 aatccagcgc cttggtggtc aagtcggcct cttacggcgc cagcgaggtg caggcgggca
     1261 aggagtttga cctcaccgcc gagattctgg ccaccgcggg caccaacgcc atcgacatgg
     1321 tgcaggtctc gctggagctg cccgaaaaga tcaccgtcgt ctccggcaac aacaattact
     1381 atgtgggcaa gctcacggcc gaggcatcca ccgcggtcac gttccgcctg ctggccagcg
     1441 cggtcaccga gcccggcagc tacaacattt cggtcaacgt ctccggcatc ggcaacgacg
     1501 gcacgccggt caacaacagc ctgaaagtca cggtgccggt ggtgcagccc gagcggttcg
     1561 agatcaccaa catggaggcg tacagccccc tgttcacggg ggaagagggc actgtctcgg
     1621 tcacctatgt gaataagggc aagggcatca tctacaacct ctcagccaaa atcgagggcg
     1681 agggcatgga caatgagggc cagaaccag
//
LOCUS       DBMDYA010000639         1708 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13944_length_1708_cov_1.413815, whole genome shotgun sequence.
ACCESSION   DBMDYA010000639 DBMDYA010000000
VERSION     DBMDYA010000639.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_1708_cov_1.413815"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            53..1051
                     /locus_tag="ACZPFK_12835"
     CDS             53..1051
                     /locus_tag="ACZPFK_12835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1407237.1"
                     /translation="MDKRKIIMDVDTGSDDAIALCAAMLSDELELLGVCTVGGNVEVK
                     NTTDNTLRVVECCGRPEVKVYRGAALPLASTLQPWGLQAQELPRREGGRDQETAVHTD
                     HLPLPPTSLREQEQSACVWLIETLLAAQDGEITLVPVGPITNLALAMRADPRILPKIR
                     EIVMMGGSHDTYPPTQAAEFNVWADPEALEIVLQSGVKVTMVSLDATSHACLDREQAK
                     LLRQIGSAPALLCAELVEHRLKASAAEGDAEGTGVGAAIHDALAVCAVLHPEVLTQVE
                     HTSCHVDLGHGYAYGETVLGRNYKFMEMPKNCYYARSADSQLFFDWLYGVLQKAAQ"
     gene            1054..1620
                     /locus_tag="ACZPFK_12840"
     CDS             1054..1620
                     /locus_tag="ACZPFK_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HIR1407238.1"
                     /translation="MKKPRREYPRFALCGLNCGLCPMYHISEQNHCTGCGGEGRPSCA
                     VLRCAEEHGGVAYCFQCPEYPCARYEPPPQYDSFLPARNVRRDFARAEDWGLTRYQAV
                     LDEKVELLRFLLRGYNDGRRKTFFCLAANLLELEDLRAAVDRLQRETGPDMELKERAA
                     LAVRLLEEAAAAAGMELKLKRPPRGGKK"
BASE COUNT          294 a          534 c          622 g          258 t
ORIGIN      
        1 tttgccgggc gccgcggtgc ggggcccggc cggatcaggg aggatgcttt aaatggacaa
       61 gagaaagatc atcatggacg tggacaccgg ctcggacgac gcgatcgcgc tctgcgcggc
      121 catgctcagc gacgagctgg agctgctggg cgtctgcacg gtgggcggca acgtggaggt
      181 gaagaacacc accgacaaca cgctgcgggt ggtggagtgc tgcggccgcc cggaggtgaa
      241 agtctaccgc ggggcggcgc tgccgctggc gtccaccctg cagccctggg ggctgcaggc
      301 gcaggagctt ccccgccgcg agggcggccg ggatcaggag acggcggtgc acaccgatca
      361 tctgccgctg ccgcccacct cgctgcgcga gcaggagcag agcgcctgcg tctggctgat
      421 cgaaacgctg ttggccgccc aggacggcga gatcacgctg gtgccggtgg gccccatcac
      481 caacctcgcc ctggccatgc gggccgaccc ccgcattttg cccaagatcc gcgagatcgt
      541 gatgatgggc ggctcccacg acacctatcc gcccacccag gcggcggagt tcaacgtgtg
      601 ggccgacccg gaagcgctgg aaatcgtgct gcagagcggg gtcaaggtga ccatggtctc
      661 gctggacgcc acctcccacg cctgcctgga ccgcgaacag gcaaagctgc tgcggcagat
      721 cggcagcgcc ccggcgctgc tctgcgcaga gctggtcgag caccggctga aagcgtcggc
      781 ggcggagggg gatgcggagg gcaccggcgt gggggcggcg atccacgacg cgctggcggt
      841 ctgtgcggtg ctgcacccgg aggtgctgac ccaggtggag cacaccagct gccatgtgga
      901 cctgggccac ggctacgcct atggcgagac ggtgctgggg cgcaactaca aattcatgga
      961 gatgccgaag aactgctatt atgcccgctc ggcggactcg cagctgtttt ttgactggct
     1021 gtacggcgtg ctgcaaaaag cggcgcagtg aagatgaaaa agccccggcg ggagtatccc
     1081 cgctttgcgc tctgtggcct caattgcggc ctgtgcccca tgtaccacat cagcgagcag
     1141 aaccactgca ccggctgcgg cggcgagggg cggccctcct gcgcagtgct gcgctgcgcg
     1201 gaggagcacg gcggcgtggc ctattgcttt cagtgccccg aatacccttg cgcgcgctat
     1261 gaaccgccgc cccaatatga ttcctttttg ccggcccgca acgtgcgccg cgattttgca
     1321 cgcgcggagg actggggcct gacccgctat caggcggtgc tggacgagaa agtggagctg
     1381 ttgcgctttt tgctgcgcgg gtacaacgac ggcaggcgca agactttttt ctgcctggcg
     1441 gccaatctgc tggagctgga ggatctgcgg gcggccgtcg accgactgca gcgggagacc
     1501 gggccggaca tggagctgaa agagcgggcg gctttggcgg tgcgcctgct ggaggaggca
     1561 gccgcggccg cggggatgga gctgaagctg aaacgcccgc cgcgcggcgg caaaaaatga
     1621 aagctccgcc ctgcgcgccg ggcaaggggg gcggacaatt gaataaaaga cgtcccgcgc
     1681 gctgcactcg gcagacgcgc gggacgtc
//
LOCUS       DBMDYA010000640         1707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13950_length_1707_cov_1.575965, whole genome shotgun sequence.
ACCESSION   DBMDYA010000640 DBMDYA010000000
VERSION     DBMDYA010000640.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_1707_cov_1.575965"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            248..1426
                     /locus_tag="ACZPFK_12845"
     CDS             248..1426
                     /locus_tag="ACZPFK_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013570.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1407239.1"
                     /translation="MTEKLFENDSYLCRFTARVERCETAKKGFAVVLDRTVFFPEGGG
                     QPADLGVLGGAAVLDVQEKDGEVLHFTDAPLAAGAQVEGAVDWARRFDLMQQHTADHI
                     FSALVHRRFGFENVGFHIGAETTHMDFSGELSARQIDELELEANRAVMANLAVTLSYP
                     GEAALAAMEFRSKKELSDLSGRVRIVTIGDLDVCACCGTHVAATGEVGLLKITGCQSY
                     KGGVRVFMAAGERAFLLAQREHHQVEAVSGLLSAKQPEIVAAVTRLKNDDADARLQLG
                     QLQGRLLACRADAYAGRRQVLAFEPDLAPDDLRRFALLLCERGADLAAVFSGGEGGYK
                     YALATGGALDARALGKEMNALLAGRGGGKPELIQGSVACTRAQAEAFFAEKGISPDRA
                     "
     gene            complement(1534..1686)
                     /locus_tag="ACZPFK_12850"
     CDS             complement(1534..1686)
                     /locus_tag="ACZPFK_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR1407240.1"
                     /translation="MFGGIKGQTEEHREIITRRAGEGWRYAGFVPVTQRSTGYIEEID
                     LVFERE"
BASE COUNT          278 a          574 c          612 g          243 t
ORIGIN      
        1 cggtcagggg gctgaaaagc gcttttcagc cccctgaccg gcggccaaga ggcgaacatg
       61 actcctccca aaaaacacgc accggcgcga acgcctatca aggggagggc gcagggcgcc
      121 gctcgttttt gacccgggcg gcagccgcgg cgccgctgca aaatcgatcc cgagcttttc
      181 ggcgcgcccg cggcggtgcg cgatcggggg cccgcccggc ggagaccggc ggagaaagga
      241 accacagatg accgaaaaac tatttgaaaa cgacagctac ctctgccgct tcaccgcccg
      301 ggtggagcgc tgcgagacgg ccaaaaaggg ctttgccgtg gtgctggacc gcaccgtctt
      361 tttccccgag gggggcggcc agcccgccga cctgggcgtg ctcggcggcg cggcggtgct
      421 ggatgtacag gagaaagacg gcgaggtgct tcactttacc gacgcgccgc tggccgccgg
      481 ggcgcaggtg gagggcgcgg tggactgggc ccgccgcttc gacctgatgc agcagcacac
      541 ggccgaccac atcttctcgg cgctggtgca ccgccgcttc ggctttgaga acgtgggctt
      601 ccacatcggt gccgagacca cccacatgga tttcagcggc gagctgagcg cccggcagat
      661 cgacgagctg gagctggagg ccaaccgcgc cgtgatggcc aatctggcgg tcacgctcag
      721 ctaccccggc gaggcggcgc tcgccgccat ggaattccgc agcaaaaagg agctgagcga
      781 cctctccggc cgcgtccgca tcgtcaccat cggcgatctg gacgtctgcg cctgctgcgg
      841 cacccatgtg gccgccaccg gcgaggtggg cctgctgaag atcaccggct gccagagcta
      901 taagggcggc gtgcgggtgt ttatggcggc gggcgagcgg gcctttctgc tggcccagcg
      961 ggagcaccat caggtggagg ccgtgagcgg cctgctgtcg gcaaaacagc cggagatcgt
     1021 ggccgcggtg acgcggctga aaaacgacga cgcggacgcc cgcctgcagc tgggccagct
     1081 gcagggccgg ctgctggcct gccgggcgga cgcctacgcg gggcggcggc aggtgctggc
     1141 ctttgagccc gatctggccc ccgacgacct gcgccgcttc gcgctgctgc tctgcgagcg
     1201 cggcgccgat ctcgccgccg ttttcagcgg cggcgagggc ggctacaagt acgcgctggc
     1261 caccggcggc gcgctggacg cccgcgcgct gggcaaggaa atgaacgccc tgctggcggg
     1321 ccggggcggc ggcaagccgg agctgattca gggcagcgtg gcctgcaccc gggcgcaggc
     1381 ggaggcgttc ttcgcagaga aaggcatctc gcccgaccgg gcctgaaaaa gcgcaggcgc
     1441 ggcctgcgat gccgcaaaaa aaggaggcgc gccggggggc catccccggc gcgcctcttt
     1501 gcatttttcc gcacaggccg cagggcgggg gaactactcc cgctcaaaga ccaggtcgat
     1561 ctcctcgata tagccggtgc tgcgctgggt caccggcaca aagccggcat agcgccagcc
     1621 ctccccggcc ctgcgggtga tgatctcccg gtgctcctcc gtctggccct tgatgccgcc
     1681 gaagatgctg tagccggtcc catagtc
//
LOCUS       DBMDYA010000641         1705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13966_length_1705_cov_1.748753, whole genome shotgun sequence.
ACCESSION   DBMDYA010000641 DBMDYA010000000
VERSION     DBMDYA010000641.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13966_length_1705_cov_1.748753"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="ACZPFK_12855"
     CDS             complement(<1..146)
                     /locus_tag="ACZPFK_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR1407241.1"
                     /translation="MAVKEKIRIRLKSYDHQLIDAAAEKIVETARRTGARVSGPIPLP
                     TDKEV"
     gene            complement(686..1432)
                     /locus_tag="ACZPFK_12860"
     CDS             complement(686..1432)
                     /locus_tag="ACZPFK_12860"
                     /inference="COORDINATES: protein motif:HMM:NF051836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjL-like protein"
                     /protein_id="HIR1407242.1"
                     /translation="MKNKATEEREKSLPRRIFAGALGLLLVLALLTGVSFVTGNPLAE
                     ALARRRAANYLSTTYPGREFTVSEVSKSGAGRLDYSMRVDETGLLDGGFFLFVPLWGE
                     VQDTRDYWVNTMRNTAQRLGNELAGAAALALEQAGFPVLERCTATMGYEPDMQESFPQ
                     MPFASRMELDMPLDPAAPPVPVRLELELVDPQPGEERARQLLDQAQTALREAGIRVDY
                     YCLTLYTKDFAPFADVGRQYQLEDVPAGQS"
     gene            complement(1429..>1705)
                     /locus_tag="ACZPFK_12865"
     CDS             complement(1429..>1705)
                     /locus_tag="ACZPFK_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407243.1"
                     /translation="YNLILMPAVGAVLYAAAGKKWYLGPCWLGALGALWQLVMLLTRE
                     RAPLGDALGPALLMGAAYLLLCLLGGGIAALLRCAFTSGKSRGERKR"
BASE COUNT          330 a          575 c          478 g          322 t
ORIGIN      
        1 acctctttgt cggtgggcag ggggatcggg ccggaaacgc gggcgccggt gcggcgcgcg
       61 gtctccacga tcttctctgc ggcagcgtcg atcagctgat gatcgtagct cttcaaacgg
      121 atcctgattt tctccttgac tgccatgaaa tcgcctcctt aaaattttgc ctggcggttc
      181 gataaatacg gaacaccgcg ccgggtgttt cacgcttttg catgaagaaa gccggtgaac
      241 cgctaaacgc agttcccccg gacacacatc acgcaaagtt cgtggaaatt gggtcccgaa
      301 aggggccata tccctttgcc atccgtattt tttcgcccgg ttctggatcg gacatactcg
      361 gcggaaaaac ccacggtaac ccgtggcaac ctcccgcgtc aacgcatatc gcagctctgc
      421 acagggtgca gagccctcgc agtcgcctaa tcataataca ataaaacccc ctgcgatgca
      481 aggggttttt gaaaaaaact tagaattttt ttgcaaattt tcgttgggat gcacaaaagg
      541 ggcagcggac ccccggcgcg ggagaaaagc tgtgcaaaag gccggtcggc accgagccgc
      601 cgctgtcttt agagtatacc gcctgtacgc cgccgctgca accctatggc ctcttcccgc
      661 cgctttttct ccgcttttcc cccgctcagc tttgcccggc gggcacgtcc tccagttggt
      721 actggcgccc cacgtccgca aagggcgcaa aatcttttgt gtacagggtg aggcagtaat
      781 aatccacccg gatgcccgcc tcccgcagcg ccgtctgggc ctgatccaaa agctgccgcg
      841 cccgttcttc cccgggctgg gggtcaacaa gctccagctc cagccgcacc gggacgggcg
      901 gagcggccgg gtccagcggc atgtccagct ccatgcggga ggcgaaaggc atctgcggaa
      961 agctctcttg catatccggt tcatagccca tcgtggcggt gcagcgctcc agcacaggga
     1021 accccgcctg ctccagcgcc agcgccgccg cgcccgcgag ctcgtttccc agccgctggg
     1081 cggtgttgcg catcgtattc acccagtaat cacgggtgtc ctggacctcg ccccagagcg
     1141 ggacgaagag aaaaaacccg ccgtcaagga ggcccgtctc gtccacccgc atggagtagt
     1201 ccaatcgccc ggcgccgctt ttgctcacct cgctcaccgt gaactcccgg cccggatatg
     1261 ttgtcgagag ataatttgcc gcccgccgcc ttgccagagc ctccgccaga gggttgccgg
     1321 tgacaaagct cacgccggtg agcagcgcca acaccagcag caggcccaaa gcgcccgcaa
     1381 aaatgcggcg gggcaggctt ttttctctct cttccgtggc tttgttcttc atctctttct
     1441 ctcccctctc gactttcccg acgtaaaagc gcagcgcagc agcgcggcga ttcccccgcc
     1501 cagtaggcag agcagcagat aggcggcgcc catcagcagg gccgggccca gggcgtcgcc
     1561 caggggcgcg cgctcccgtg tcagcagcat caccaactgc cagagcgcgc ccagcgcgcc
     1621 cagccagcag gggcccagat accacttttt tcccgccgcc gcatagagca ccgcccccac
     1681 agcgggcatg aggatcagat tataa
//
LOCUS       DBMDYA010000642         1705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13969_length_1705_cov_1.431421, whole genome shotgun sequence.
ACCESSION   DBMDYA010000642 DBMDYA010000000
VERSION     DBMDYA010000642.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13969_length_1705_cov_1.431421"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="ACZPFK_12870"
     CDS             complement(<1..245)
                     /locus_tag="ACZPFK_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR1407244.1"
                     /translation="MAFLLLFPFFLLRFGLLSLLSREAVARAARFAPLKEGERWAYWV
                     YQASNAALLMGLLFSRVRSSPSWLFFTAAVVCGVGLLL"
     gene            complement(567..1130)
                     /locus_tag="ACZPFK_12875"
     CDS             complement(567..1130)
                     /locus_tag="ACZPFK_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1407245.1"
                     /translation="METRIRPYGESDLMAIQKIWNSVVEGGDAFPGETPLSLQECRDY
                     FARQSLTAVAETEGRVAGLYILHPNNIGRAGHIANASYAVASSARGRGIGEALVRHSL
                     SQLRPHGFTGLQFNAVVSTNRTAIALYEKLGFTRVGTIPGGFRLPDGSFVDIYLYFHA
                     APEGDGARPQGVRPHGAAGQGGAAGEA"
BASE COUNT          295 a          615 c          529 g          266 t
ORIGIN      
        1 agcaaaaggc ccacaccgca gaccaccgcc gccgtgaaaa acagccagga cggagaagac
       61 ctgacgcgcg aaaaaagcaa ccccatgaga agcgctgcgt tggaggcctg gtacacccag
      121 taggcccatc tctccccctc tttcagcggc gcaaaacggg cggcgcgcgc caccgcctct
      181 cggctgagca gggacaaaag gccaaaacgc agcagaaaaa aaggaaacag cagcaaaaag
      241 gccatcgggc cctcctctct tttgcgccag ggcctcatac ctcggcagcg gcatggaagc
      301 ggctttgctt ttagacgggg agcccccccc accgacgcgg caggcaggcg cggcaaacgc
      361 ctttctccgc agcatggcgg cggcagacgc ggcggcatgt cgtctttccg cgccctctcc
      421 cggccgctgc ttttctccgc caaagccgga gattttgaga acatcaaaag cgcacggcgg
      481 gcgattttct tcccggcgcg tgcggcggca gaaacctttc tccccggcac gggggcttct
      541 gcggcgcgct tcgcgggctt tccgccttac gcctcgccag ccgccccgcc ttgccctgct
      601 gcgccatggg gccggacgcc ctgcgggcgc gcgccgtccc cctcgggcgc ggcgtgaaaa
      661 tagagataga tgtccacaaa actgccgtcc ggcagccgaa agccgcccgg gattgtgccc
      721 acgcgggtaa agcccagctt ttcgtaaaga gctatggccg ttcgattggt gctgaccacc
      781 gcgttgaact gcagccccgt gaacccgtgg gggcgcaact ggctgaggct gtgccgcacc
      841 agcgcctccc cgatgccccg gccgcgcgcg gaggacgcca ccgcatagct ggcgttggcg
      901 atatgtcccg cgcggccgat gttgttgggg tgcaggatgt agaggcccgc cacccggccc
      961 tccgtctcgg ccaccgccgt caggctctgc cgcgcaaaat aatcccggca ttcctgcagg
     1021 ctcagcggcg tctcgccggg gaaagcgtcc cctccttcca ccacgctgtt ccagatcttt
     1081 tgaatggcca tcaggtcgct ctcgccgtag ggccgtatcc gcgtctccat ctctttttcc
     1141 tcctccgtct tttcgctgcc gctcccctgc tttcgccccc ggcccgcgca ccccggggaa
     1201 ttttcgccgg gatgtcggct gcggagagga cggcgccgcg ggtcccgacc cgcgtgcccg
     1261 caaaaaatcc cgcccgctcc ggcccccgca aaaaacggcg ggtggcggca aggcccggcc
     1321 ccgacgcacc ctccgggcgg cccggcaaac cggcgcctcc cgccgctgca ggcagtccct
     1381 ctaccgccct gagggcagcc gggacgcggg ccgcaggacc ttgcaaacgc ccggcccacc
     1441 ggcaaaagcc gggctgggcc acggggccgc cgctgtgcag aaagctttga gatttattat
     1501 accacagacg cgaagcgcag tggtataatt ggccatccaa aagcgattgt ccccggaggg
     1561 ggacgagccg ctggccatct tttggtatag tgcccgcttt gctgctgttc gaatacgcgc
     1621 aaaccgcggc gggggcgctg cggtttgcga ccggcgcgcc caaccgttca gacacgggcc
     1681 caaccggtga ggcgggctgt cggca
//
LOCUS       DBMDYA010000643         1705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13970_length_1705_cov_1.339152, whole genome shotgun sequence.
ACCESSION   DBMDYA010000643 DBMDYA010000000
VERSION     DBMDYA010000643.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13970_length_1705_cov_1.339152"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="ACZPFK_12880"
     CDS             complement(<1..1526)
                     /locus_tag="ACZPFK_12880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1407246.1"
                     /translation="MEPFEDILSRAEETLRGWFCKKDSRSTLEAFDRRAVVWTGMDEE
                     GSAHGYAQLERLFSSNRDKIRPRALSGLHLEVCGRGEGFCVVTGSFDLQVVDTAERDH
                     CRLTLVYTAGENGPKIIHGHVSVPGLPAGVRAHSGEPVSWAEYERLRLAFEEKAAQIE
                     MIARTTAGGMKGSLDDDKYTYFYVNDELCEMLGYTHDEFMEMSGGTAVGAVYPPDLPA
                     ALASVAECFAKGPEYHAEYRIRKKDGSLLWVMDSGRKVKNARGEWVINSIITDISALK
                     EAAEQLRIERERYEIVAELAGETIFEYDLGADTLQEFLPAHAGQAPSRRCYTGLRSGE
                     AMGTLGLHPDDQQRLLEKFSRAEHSPQPEDLTTYECRRLLPDGSYGWRRILLRFICDR
                     DGRPIRAVGKVVDISSERLLLQKSRTDALTGAYNRAYLEHAVEKYLAQKPEEVFAACL
                     MIDIDHFKQLNDSCGHLCGDTVLTDLVGMLKRLYRATDLVARLGGDEFMVFMKDIYSV
                     "
BASE COUNT          299 a          573 c          475 g          358 t
ORIGIN      
        1 tcgacgctgt agatgtcttt catgaagacc atgaactcgt ccccgcccag gcgcgccacc
       61 aggtcggtgg cgcgatagag ccgtttcagc atccccacca gatcggtgag caccgtgtcc
      121 ccgcacagat gcccgcagct gtcgttgagc tgcttgaagt ggtcgatgtc gatcatcaga
      181 caggcggcaa agacctcttc cggcttctgc gccagatatt tttccaccgc atgctccaga
      241 taggcccggt tgtacgcccc ggtgagggcg tcggtgcgcg atttttgcag cagcagccgc
      301 tctgagctga tgtccaccac cttgcccacc gcccggatgg gccgcccgtc ccggtcgcag
      361 ataaagcgca gcaggatgcg gcgccagccg tagctgccgt ccggcagcag gcggcggcac
      421 tcataggtgg tcagatcctc cggctggggg ctgtgttcag cccttgaaaa tttttcaagc
      481 aggcgctgtt gatcgtcggg atgcagcccc agcgtgccca tggcctcgcc gctgcgcagc
      541 ccggtatagc atctgcggct tggcgcctgc ccggcgtgcg cgggcagaaa ctcctgcagc
      601 gtatcggcgc cgaggtcgta ttcaaagatc gtctcccccg ccagctccgc cacgatctcg
      661 taccgctccc gctcgatgcg aagctgctcg gccgcctcct tcagcgccga gatatcggtg
      721 atgatgctgt tgatcaccca ctcgccgcgc gcgtttttga ccttgcggcc gctgtccatc
      781 acccacagca gggagccgtc ttttttgcgg atgcggtact cggcatgata ctcgggcccc
      841 ttggcgaagc attccgccac cgaggccagc gcagccggca ggtccggcgg gtacacagcg
      901 cccaccgccg tgccgccgct catctccata aactcgtcgt gggtatagcc cagcatctcg
      961 cacagctcgt cgttgacgta aaagtaggtg tatttgtcgt cgtccaggct gcctttcatg
     1021 ccgccggcgg tggtgcgggc gatcatctcg atctgcgccg ctttctcttc aaacgcgagg
     1081 cgcaggcgct cgtattccgc ccagctcacc ggctcgccgc tgtgcgcgcg cacccccgcg
     1141 ggcagacccg gcaccgagac gtgcccatgg atgatctttg ggccgttctc ccccgccgtg
     1201 tacaccaggg tcagacggca gtggtcccgc tccgccgtgt ccacgacctg cagatcaaag
     1261 ctgccggtca ccacacaaaa tccctctccg cggccgcaga cctccaggtg caggcccgac
     1321 aaggcgcgcg ggcgtatttt gtcccggttg ctggagaaga gacgctccag ctgagcatag
     1381 ccatgcgcgg acccttcttc atccattccg gtccagacca ccgccctgcg gtcgaacgcc
     1441 tccagggtgg agcggctgtc ctttttgcag aaccaccccc gcagggtctc ctccgcccgg
     1501 gagagaatgt cctcaaaagg ctccacgccg ccacctcctt ttatttccat agccattata
     1561 aactatcata tcattttttc ccagcagaag atagcacgcc tcaaaatttt ttgttttttt
     1621 gtcctccatg caaaaagaag cctattttta tgaaaaattt taggttaaag tcttgaaaag
     1681 agagagcggc ttttttaccg cctgt
//
LOCUS       DBMDYA010000644         1704 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_13978_length_1704_cov_1.417966, whole genome shotgun sequence.
ACCESSION   DBMDYA010000644 DBMDYA010000000
VERSION     DBMDYA010000644.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13978_length_1704_cov_1.417966"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1704)
                     /locus_tag="ACZPFK_12885"
     CDS             complement(<1..>1704)
                     /locus_tag="ACZPFK_12885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1407247.1"
                     /translation="RWAVMGVVQKSDLLAFSSSFRMLLFAALLLSSVVGVIGIVFVAR
                     QATKRIGSLAVNLRSADPRQPIRLAEVGVAEIDQLSEAIVSLSTQVADNAQKMSRILD
                     MAGIEIGVFEQEEDGAGFYYTDKLPGILGIRPGERFDSDVLRRRLEALPCQVEQGEQA
                     RYLFTLNGEDGVHWVRLQTVARGANILGVLIDVTKEISEKQKLEHERDYDLLTGLFNR
                     RAFQSRIEQMLARPEEVGMAVLVMMDLDNLKYVNDTYGHDFGDSYIRAAARALRRSTP
                     DKSLAARLSGDEFVAFLYGYDNMEGAREACRGIEQELRRSILALPDGSRLPVRASAGL
                     SFFPKDAVEYAPLLQYADFAMYQVKHTDKGQFTEFNIATYNRDAYLLQCREELNLLIE
                     KERLDYQFQPIVDAVTGGIFGYEALMRPATENIHSPAQLLELARSQSKLSQIERLTMF
                     RAMECAVAQGVIATGSHIFINSIPNQVLSDQELTRMEERFGRYLDRLVIELTEEERMD
                     PEKTVKKQAFAKKWGASLALDDFGVGYSGEGILVDLAPQFVKIDISLVQGIERDEDRQ
                     A"
BASE COUNT          308 a          555 c          511 g          330 t
ORIGIN      
        1 gagcctgacg gtcttcatcc cgctcgatgc cctgtaccag ggaaatatcg atcttgacga
       61 actgcggggc cagatcgacc aggatgccct cgccgctgta gcccacgccg aaatcgtcca
      121 gggccaacga cgcgccccat tttttggcga acgcctgctt cttcaccgtc ttttcagggt
      181 ccatccgctc ctcttcagtc agctcgatca cgagccggtc cagatagcgg ccaaagcgct
      241 cttccatgcg ggtcagctcc tggtccgaca gcacctggtt ggggatcgaa ttgataaaga
      301 tgtggctgcc ggtggcgatc actccctggg cgacggcgca ttccatggcg cggaacatgg
      361 tcagccgctc gatctgtgag agcttggatt gggagcgggc gagctccagc agctgcgccg
      421 ggctgtggat gttctccgtg gccgggcgca tcagcgcctc atagccgaaa atccccccag
      481 tcacggcgtc cacaatgggc tggaactgat agtccagccg ctccttttcg atcaacagat
      541 tgagctcctc gcggcactgg agcaggtagg cgtcccggtt ataggtcgcg atgttgaact
      601 cggtgaactg ccccttatcg gtgtgcttga cctggtacat ggcaaaatcg gcgtattgca
      661 gcagcggcgc atattcgact gcgtctttgg ggaagaaaga gaggcccgcc gaggcgcgca
      721 ccggcagacg gctgccgtcc ggcagggcga gaatactgcg ccgcagctcc tgctcgatgc
      781 cgcggcaggc ctcccgcgcc ccctccatat tgtcatagcc atagagaaag gcgacgaact
      841 catcgccgga gagccgcgcc gcgaggcttt tatccggtgt gctgcggcgc agagcgcggg
      901 cggcggcgcg gatatagctg tcgccaaaat cgtgcccata agtatcgttg acgtatttca
      961 ggttatccag gtccatcatc accagcaccg ccatgcccac ctcttcgggg cgggccagca
     1021 tctgctcgat cctgctctgg aacgcgcggc ggttgaacag cccggtcaac aggtcatagt
     1081 cccgctcatg ctccagcttt tgtttttcgc tgatctcctt ggtgacgtcg atcagcacgc
     1141 ccaggatgtt cgccccgcgc gccacggtct gcagcctgac ccagtgtacg ccgtcctccc
     1201 cgttaagggt gaacagatag cgggcctgtt ccccctgctc tacctggcag ggcagcgcct
     1261 ccaaccgtcg gcgcaggaca tcgctgtcga atctctctcc cggccgaatc cccaggatgc
     1321 ccggcagctt gtcggtgtaa tagaatcccg ccccgtcttc ttcctgctcg aaaacgccga
     1381 tctcaatgcc cgccatatcc aggatgcggg acattttttg ggcgttgtcc gccacctggg
     1441 tgctcagcga gacgattgcc tcgctgagct gatcgatctc ggcgaccccc acctcggcca
     1501 ggcggatggg ctgccggggg tcggcgctgc gcaggttgac cgccagcgag ccgatgcgct
     1561 tggtcgcctg gcgggccaca aagacaatgc cgatgacccc caccacggag gagaggagca
     1621 atgcggcgaa cagcagcata cggaagctgg aggaaaaggc gagcaggtcg cttttctgca
     1681 ccacgcccat caccgcccag cgct
//
LOCUS       DBMDYA010000645         1701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14013_length_1701_cov_1.524375, whole genome shotgun sequence.
ACCESSION   DBMDYA010000645 DBMDYA010000000
VERSION     DBMDYA010000645.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14013_length_1701_cov_1.524375"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="ACZPFK_12890"
     CDS             <1..212
                     /locus_tag="ACZPFK_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407248.1"
                     /translation="RKKKCKPVRGRYGMWPAARRAICAALGEMNGGMKQKQYTAPGVS
                     NAFFGFIFLFFPLNAAKSFELFFRK"
     gene            346..>1166
                     /locus_tag="ACZPFK_12895"
     CDS             346..>1166
                     /locus_tag="ACZPFK_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1407249.1"
                     /translation="MKKVLSLILSAALVFSLAACGGSGSSSGSAPVAGAASGASSAST
                     GGEAPLIGYSLNSMDSTMKQMADFFEETAKAAGWEPVLLNANGDVSAELANVESLINM
                     GCKAVVIMGCDNEGSAPAVKACGDAGVPCIIAGRAINADEASYYTFVSGDHHLAGEVQ
                     AKFVNDYLAANPDEVLNIGFIYGQSGSSSVAARYQGFEDDCLSLPENEGRVNLVAEQY
                     AEFDAEKAYNITTDMLSTHPEINCFVTQTDDQAGGIINAVKAQGLSVEDYLIVSI"
     assembly_gap    1167..1176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1177..1396
                     /locus_tag="ACZPFK_12900"
     CDS             <1177..1396
                     /locus_tag="ACZPFK_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407250.1"
                     /translation="IDCSSTGIDYLKSGELDATVLMAMRQLAVTAADYVQKALDGESV
                     EKANPIPEYYQLATAENVDELLKANGMA"
     gene            1535..>1701
                     /locus_tag="ACZPFK_12905"
     CDS             1535..>1701
                     /locus_tag="ACZPFK_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407251.1"
                     /translation="MKLLEAKGINKRFYSTCALSEVDFDLEEGEVLALAGENGAGKST
                     MMNVLLGIHRP"
BASE COUNT          359 a          524 c          483 g          325 t
ORIGIN      
        1 gccggaaaaa gaaatgcaaa cccgtgcgcg gcagatatgg tatgtggcct gccgctcggc
       61 gcgccatatg tgccgcactg ggggaaatga atggcggaat gaagcaaaaa caatacacgg
      121 cccccggcgt ttcaaatgcg ttttttggct ttattttttt gttttttcca ctaaatgccg
      181 cgaaaagctt tgagctattt ttccgaaaat agaaaaccgg tcttgacttt ccgcagcgct
      241 tcccctattc tttagacaat gtcatttgca aaagttgttt cttctttttt ccaaacaact
      301 tgcagaccac aaaacacctt tcaactgaat ttggaggaaa acagcatgaa aaaagtcctg
      361 tcactgattc tctctgccgc tctggtattc tccctggccg cctgcggcgg ctcaggctct
      421 tcctcgggca gcgcccccgt cgcaggggcc gcctcgggcg cctcctccgc ctccaccggc
      481 ggcgaggccc cgctgatcgg ctattctctc aactccatgg acagcaccat gaagcagatg
      541 gcggactttt ttgaggagac cgccaaggcc gccggctggg agccggtgct gctgaacgcc
      601 aacggcgacg tctcggccga gctcgccaac gtggaatccc tgatcaacat gggctgcaag
      661 gccgttgtga tcatgggctg tgacaacgag ggcagcgccc ccgccgtcaa ggcctgcggc
      721 gatgccggcg tgccctgcat catcgccggc cgcgccatca acgccgacga ggcgtcctat
      781 tacacctttg tctccggcga ccaccatctg gccggcgagg tgcaggcgaa gttcgtcaac
      841 gattatctgg ccgccaaccc ggatgaggtg ctgaacatcg gctttatcta cggccagtcc
      901 ggcagctcca gcgtcgcggc ccgctaccag ggctttgagg acgactgtct gagcctgccc
      961 gagaacgagg gccgggtcaa cctggtcgcc gagcagtacg ccgagttcga cgccgaaaag
     1021 gcctataaca tcaccaccga catgctctcc acccacccgg agatcaattg cttcgtcacc
     1081 cagaccgacg accaggccgg cggcatcatc aacgccgtca aggcgcaggg cctgtctgtc
     1141 gaggattacc tcatcgtctc catcgannnn nnnnnncatc gactgctcca gcaccggcat
     1201 cgactatctc aagagcggcg agcttgacgc caccgtgctg atggcgatgc gccagctggc
     1261 cgtcaccgcc gccgactatg tgcagaaagc tctggacggc gagagtgtgg aaaaggcaaa
     1321 ccccatcccc gaatactatc agctggccac cgccgagaac gtcgacgagc tgctgaaagc
     1381 caacggcatg gcctgagccg aaaagacctg agccttaccg gggagcagca atgttccccg
     1441 gtacctttat cctgcgggcc cccgatcggg cgcggcgtgg ggtgcgcccc gcgtatgacc
     1501 ttcgacgcgt atgacctgcg aaaggtggaa aacgatgaaa ctactggaag caaaggggat
     1561 caacaaacgg ttctattcga cctgcgccct ctcggaggtg gactttgacc tggaggaggg
     1621 cgaggtgctg gcgctggccg gtgaaaacgg ggccggcaaa tccacgatga tgaacgtgct
     1681 gctgggcatt catcggccgg a
//
LOCUS       DBMDYA010000646         1701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14015_length_1701_cov_1.090000, whole genome shotgun sequence.
ACCESSION   DBMDYA010000646 DBMDYA010000000
VERSION     DBMDYA010000646.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14015_length_1701_cov_1.090000"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1701
                     /locus_tag="ACZPFK_12910"
     CDS             <1..>1701
                     /locus_tag="ACZPFK_12910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1407252.1"
                     /translation="ELVLPGDLPAVEAALRAQLAAGDEVRLSFRLASPPGAAPATVSC
                     SGRRAGGLLWAALALAPRDFYQGYEQLTQWYKGFQALLETSGQQAYEWDLRARTLSEA
                     GFQSLLWRELMGAEPLLAVDEDGIPSSENVYLADVPAFRRLHRSVLAGGAGGSVELRM
                     RTSYGTYRWCRLTLATLYDLEGKPRRAVGLLADIDEEKREALALAQRNERDQLTGLLN
                     GGTIRARLLTALEKLESGYIALYVVDIDHYQQVTDRLGILFGEAVLIGAADKLSAAAG
                     EGAVCGRIGATTLAVYRRAASRREAVALGRGFCVKLRQSLADGKQELCITASVGMVLI
                     PPGEKDFSLLLRQADLALAAAKRAGGGRLVAYRRSIRDDGADAGFYFGEAPAAAGSAK
                     SFNENMFYLFFNMLYEGEDPDRAVDLVLRTAGQHFHVSRAYIYEAEPEDRQVLRLTHE
                     WCDEETESLGGRLETFSIAGESAAERAARAGVYQCDDTGAPGALPRGILPRVQESGIR
                     AFLQCGILDRGLYRGCIGFDDCVAPRIWTDEEAETLARVARIVGGYLLRIRAQERRAR
                     "
BASE COUNT          231 a          565 c          643 g          262 t
ORIGIN      
        1 agagctggtg ctgcccgggg acctgccggc ggtggaggcg gccctgcgcg cccagctggc
       61 cgccggggac gaggtgcgac tctcctttcg gctggcctcc cccccgggcg ccgcgccggc
      121 gacggtcagc tgctcaggcc gccgggccgg cggcctgttg tgggcggcgc tggcgctggc
      181 accccgcgac ttttatcagg ggtatgagca gctcacccag tggtacaagg gcttccaggc
      241 tttgctcgag acctccggcc agcaggccta cgagtgggac ctgcgcgcgc gcaccctgtc
      301 ggaggccggg tttcagtcgc tgctctggcg tgagctgatg ggggccgaac cgctgctggc
      361 ggtggacgag gacggcatcc cctccagcga aaacgtctat ctggccgatg tgcccgcctt
      421 tcggcggctg caccgcagcg tgctggcggg cggcgcgggc ggcagcgtcg agctgcgcat
      481 gcgcaccagc tacggcacct accgctggtg ccgcctgacc ctggcgaccc tctacgacct
      541 ggagggcaag ccccggcgcg ccgtgggcct gctggcggat attgacgagg aaaagcgcga
      601 ggcgctggcg ctggcccagc gcaacgagcg ggaccagctc accggcctgc tcaacggcgg
      661 caccatccgc gcccggctgc tcacggcgct ggaaaagctg gagagcggct atatcgcgct
      721 gtatgtggtg gatatcgacc actatcagca ggtcaccgac cggctgggca tcctgttcgg
      781 cgaggcggtg ctgatcggcg ctgcggataa gctgtccgcg gcggcgggcg agggagccgt
      841 gtgcgggcgc atcggcgcca ccaccctggc ggtctaccgg cgggccgcct cgcgccgcga
      901 ggcggtggct ttgggccgcg gcttctgcgt aaagctgcgc cagagcctgg ccgacggcaa
      961 gcaggagctc tgcatcaccg ccagcgtggg gatggtgctg atcccgcccg gcgaaaagga
     1021 tttttctttg ctgctgcggc aggcggacct ggcgctggcg gcggccaagc gggcgggcgg
     1081 cgggcggctg gtggcctacc ggcgcagcat ccgcgacgac ggggcggacg cggggttcta
     1141 tttcggcgag gccccggccg cggccggctc ggcaaagtcg ttcaacgaga acatgttcta
     1201 cctgtttttc aatatgctct atgagggcga ggacccggac cgcgccgtcg acctggtgct
     1261 gcgcaccgcg gggcagcatt tccatgtttc ccgcgcttat atctatgagg cggagccgga
     1321 ggaccggcag gtcctgcggc tgacccatga gtggtgcgac gaggagaccg aaagcctcgg
     1381 cggcaggctg gagacctttt ccatcgccgg cgagagcgcc gccgagcggg ctgcccgcgc
     1441 gggggtctac cagtgcgacg acaccggcgc gccgggcgcc ctgccccggg gtatcctgcc
     1501 gcgggtgcag gagagcggca tccgcgcctt tttgcaatgc ggcatcctgg accggggcct
     1561 ttaccgcggc tgcatcggct ttgacgactg cgtggccccg cgcatctgga ccgatgagga
     1621 ggccgagacg ctggcacgcg tggcgaggat cgtgggcggc tatctgctgc gcatccgcgc
     1681 gcaggagcgg cgggcccgcc a
//
LOCUS       DBMDYA010000647         1699 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14035_length_1699_cov_1.521902, whole genome shotgun sequence.
ACCESSION   DBMDYA010000647 DBMDYA010000000
VERSION     DBMDYA010000647.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14035_length_1699_cov_1.521902"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="ACZPFK_12915"
     CDS             complement(<1..1063)
                     /locus_tag="ACZPFK_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1407253.1"
                     /translation="MEKAKVYYSDLRALPGTNLLVKLQRLIRRAGIGEIDMKNKMVAI
                     KIHFGEPGNLSYLRPNYAKAVADVVKELGGMPFLTDCNTLYVGRRKNALDHIDAAYEN
                     GFTPFSTGCNVIIADGLKGTDDVEVPVVGGEMVQTAKIGRAIMDADVFLTLSHFKGHE
                     STGFGGCLKNIGMGCGSRAGKMEQHSSGKPSVDHEVCVGCRTCAKNCAHGAISFGEDR
                     KATIDHAKCVGCGRCLGACNFDAIYNENYSANDELNKKIAEYSKAVVDGRPHFHISLV
                     MDVSPYCDCHAENDAPIIPDVGMFASFDPVALDQACADACNQMPVMPGSLLDDEMHGE
                     QFCDHHDHFTNTTPETNWKT"
     gene            1501..>1699
                     /locus_tag="ACZPFK_12920"
     CDS             1501..>1699
                     /locus_tag="ACZPFK_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1407254.1"
                     /translation="MYYTIRQVAQRTGIPASTLRFYETRGLLRRVRRDAHGVRAYSDA
                     DLDRLGTITCLKNTGMGIGEMA"
BASE COUNT          287 a          514 c          553 g          345 t
ORIGIN      
        1 aggtcttcca attcgtctcg ggggtggtgt tggtgaaatg gtcgtggtgg tcgcagaact
       61 gctcgccgtg catctcgtcg tccagcaggc tgccgggcat caccggcatc tggttgcagg
      121 cgtcggcgca ggcctggtcc agcgccaccg ggtcaaagct ggcgaacatg cccacgtccg
      181 ggatgatggg cgcgtcgttc tcggcatggc agtcgcagta gggggagaca tccatcacca
      241 ggctgatgtg gaaatggggc cgcccgtcca ccacggcttt cgagtactcg gcgatttttt
      301 tgttcagctc gtcgttggcg gaataatttt cattgtagat ggcgtcaaag ttgcaggcgc
      361 ccaggcagcg gccgcagccc acgcactttg cgtggtcgat ggtggccttg cggtcctcgc
      421 cgaagctgat tgccccgtgg gcgcagtttt tggcgcaggt gcggcagccc acacagacct
      481 cgtggtccac gctgggcttg ccagagctgt gctgctccat cttgccggcg cggctgccgc
      541 agcccatgcc gatgtttttc aggcagccgc cgaagccggt ggactcatgc cccttgaagt
      601 gagacagggt caaaaagacg tccgcgtcca tgatggcgcg gccgatcttg gccgtctgca
      661 ccatctcgcc gcccaccacc ggcacctcca cgtcgtcggt gcccttgagg ccgtcggcaa
      721 tgatcacgtt gcagccggtg gaaaacgggg tgaagccgtt ttcataggcc gcgtcgatgt
      781 ggtccagggc gtttttgcgc cggcccacat acagggtgtt gcagtcggtc aaaaagggca
      841 tgccgcccag ctccttgacc acgtcggcca ccgccttggc atagttgggt cgcagataag
      901 acaggttgcc cggctcgccg aaatggatct tgatggccac catcttgttt ttcatgtcga
      961 tctcgccgat ccccgcacgg cggatcagcc gctgcagctt taccagcagg ttggtgccgg
     1021 gcagggcccg cagatcgctg taatagacct ttgctttctc catcttgcat tgctcctttt
     1081 caccggccgt tttgcacggc tcgcgtctcg ttgccgggga aaacaagaaa aagacgccgc
     1141 caaagcgccg ctcgcaccgc gccgcccgca ccggctgggg cgcggggagg gataaacgcc
     1201 tctgctcggc gcgccgcggg tctgtgcccg cccttgtgct ggcgccgcgc ggcgaagccg
     1261 gcgctttctt gtttggcccc gggttttgaa tgaggctgtt tttattgtac ggcagaacgc
     1321 catgcagggc aagggacccg gcctcaaaaa ttccccaaaa acaaaagccg cgccggagct
     1381 ttgtccggcc ccggccgagg gcgcgcctcc ccttgcaggg cggcccgctt tgggctaaaa
     1441 taactataag ccttaaagta aggtttaagt caagagcttt tttaaaaggg ggagagggct
     1501 ttgtattaca ccatcaggca ggtcgcgcag cgcaccggca tcccggcctc gacgctgcgt
     1561 ttttacgaga cgcgggggct gctgcggcgg gtgaggcggg acgcgcatgg ggtgcgggcc
     1621 tacagcgacg cggatctgga ccggctgggc accattacct gcctgaagaa cacgggcatg
     1681 ggcatcgggg agatggcgc
//
LOCUS       DBMDYA010000648         1692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14110_length_1692_cov_1.739158, whole genome shotgun sequence.
ACCESSION   DBMDYA010000648 DBMDYA010000000
VERSION     DBMDYA010000648.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14110_length_1692_cov_1.739158"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="ACZPFK_12925"
     CDS             complement(<1..184)
                     /locus_tag="ACZPFK_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR1407255.1"
                     /translation="MDAKIEAFTAQPQDEGARLDSFLSAALAGATRSMAQQLIAAGNV
                     LLNGAPAPKSARLRAGD"
     gene            complement(174..692)
                     /gene="lspA"
                     /locus_tag="ACZPFK_12930"
     CDS             complement(174..692)
                     /gene="lspA"
                     /locus_tag="ACZPFK_12930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1407256.1"
                     /translation="MLFTIISIAAMVGLDQLTKYWATLALAPDKTAPFLPGVMELRYV
                     LNDGAAFSVFSGKVWGQWFLIAVTSAALLAVLYVLFFKRARLDKLGYTALLLIEAGGI
                     GNLIDRVLHGYVVDFFATTFVDFAVFNVADCFVSVGVVLLAAYYIRSELAAKKARPKA
                     QGAPDEGSGDGR"
     gene            complement(821..>1692)
                     /locus_tag="ACZPFK_12935"
     CDS             complement(821..>1692)
                     /locus_tag="ACZPFK_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1407257.1"
                     /translation="DVRISKEILKQLSEAYRKIRNTARFILGNLNGFTPDADQVEDAQ
                     LEELDRWALASLDRLVAECRAGYDAYDFSKVYHAVHKFCVVDMSNFYLDVIKDRLYIN
                     KADSVKRRAAQTAMFRILVSLTKLIAPILAFTSQEIWEAIPAFAGKQKYVACEDIDLP
                     GLRTLSDEEWAKWDEIIAIRDDVRKALEDARAAKVIGASLEAKVTLNCSGEVYAFAKP
                     LEDRLAELCIVSKVCVEQGDEGRKGDVDGLAVAVSRAEGEKCARCWMYTEDVGSHAAH
                     PTLCARCAAIIES"
BASE COUNT          314 a          556 c          513 g          309 t
ORIGIN      
        1 cgtctccggc gcgcagacgc gcgctcttgg gggccggggc gccgttgagc agcacattgc
       61 ccgccgcgat caactgctgc gccatgctgc gggtcgcccc cgccagcgca gcggacaaaa
      121 agctgtccaa ccgcgcgccc tcgtcctgcg gctgcgcggt gaacgcttct attttagcgt
      181 ccatccccgc tcccctcgtc cggcgccccc tgcgctttcg gccgcgcctt tttagcggcc
      241 agttcgctgc ggatataata ggcggccagc agcaccacgc cgacgctgac aaagcaatcc
      301 gccacattga acacggcaaa atccacaaaa gtggtggcga aaaaatccac cacatagccg
      361 tgcaggaccc ggtcgatcag gttgccgatg ccgcccgcct cgatgagcag cagggccgtg
      421 tagcccagct tgtccagccg ggcgcgcttg aagaacaaca cgtagagcac cgccagcagg
      481 gccgccgagg tgacggcgat caaaaaccac tgtccccaga ccttgcctga gaacacgctg
      541 aacgccgcgc cgtcgttgag cacatagcgc aattccatga cgcccggcaa aaagggcgcg
      601 gtcttgtcgg gggcgagcgc aagggtcgcc cagtacttgg tgagctggtc caagccgacc
      661 atggcggcga tggagatgat ggtaaacagc atagattcct ttgcctttcc tcccccttgc
      721 gggggcgtga ttttgaggta ttggcgcgat cgctctgaat tgcaaaatag cggtgcatcg
      781 cctgcaccgc tattttgagc gcggaaagac cgccctattt tcaggattcg atgatcgcgg
      841 cgcagcgggc gcagagcgtg gggtgggccg catgactgcc cacatcctcg gtgtacatcc
      901 agcagcgggc gcacttttcg ccctccgcac ggctcaccgc cacggcaagg ccgtcaacgt
      961 cgcccttgcg gccctcgtcg ccctgctcca cacagacttt tgagacgatg cacagctcgg
     1021 ccagccggtc ctccagcggc tttgcaaagg catagacctc gccgctgcag ttgagggtga
     1081 cctttgcctc cagcgaggcg ccgatcacct tggctgcgcg ggcatcctcc agcgctttgc
     1141 gcacgtcgtc gcggatggcg atgatctcat cccacttcgc ccactcctcg tcgctcagcg
     1201 tgcgcaggcc cggcaggtcg atgtcctcac aggcaacata cttctgcttg ccagcaaagg
     1261 cggggatggc ctcccagatc tcctgcgagg taaacgccag gatgggggcg atgagcttgg
     1321 tcagcgagac caggatgcgg aacatcgcgg tctgcgccgc acggcgcttg acgctgtccg
     1381 ccttgttgat gtacagacgg tccttgatga cgtcgagata gaagttggac atatccacca
     1441 cacagaactt gtgcaccgcg tgatagacct tgctgaagtc ataggcgtcg taacccgcgc
     1501 ggcactcggc caccaggcgg tccagcgagg ccagggccca gcggtccaac tcctccagct
     1561 gggcgtcctc cacctggtct gcatccggtg taaagccgtt caggttgcca aggatgaagc
     1621 gggccgtatt gcggatcttg cggtaggcct cggagagctg cttgaggatc tccttgctga
     1681 tgcgcacgtc ga
//
LOCUS       DBMDYA010000649         1691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14131_length_1691_cov_1.234591, whole genome shotgun sequence.
ACCESSION   DBMDYA010000649 DBMDYA010000000
VERSION     DBMDYA010000649.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14131_length_1691_cov_1.234591"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            354..>1691
                     /locus_tag="ACZPFK_12940"
     CDS             354..>1691
                     /locus_tag="ACZPFK_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1407258.1"
                     /translation="MQKQDQDVIRVTGATENNLKGVSLEIPKHKITVFTGVSGSGKSS
                     LVLDTIAAQSRRELNETFPSFVQHYLPKYGRPHVERVENLPAAIVIDQRKPAPNARST
                     VGTYTDIYTLLRLLFSRVGTPFVGYSDAFSFNHPQGRCPRCDGLGEVEELDVHKLVDF
                     DKSLDEAGCIHYIAFDPGQWRWVRYANSGLFDLDKKIRDYTPEELELFLYTPPIKLKD
                     PPPGWPKTAKYEGLVHRMYRSIIRSEEGKLHRKLLEPMVASGVCPDCGGARLNQKVLA
                     CRIGGKNIADAAATPLRELRAWAASISDPLAEDLKKLLAERLSALIEIGLDYLTLDRG
                     MGTLSGGEAQRCKIAKYNNSALSDLLYILDEPSVGLHSHDIHLLRSAVERLRDGGNTV
                     LLVEHHREMIRVADHLVDMGPGAGGAGGEILFEGGYEELLHGGTALTGRLLRAH"
BASE COUNT          293 a          566 c          575 g          257 t
ORIGIN      
        1 tcgtcccgct ctggacagag ccgaaaaaga ggcttttccg caagggcgac gggcaggcgc
       61 aagccgtttt gtgagcggcg ccgcggccct gggcgaggcc gcccgcctct ctgtgcatcg
      121 gctggggacg gcgctgggcc gaggctgggt tccctgcggg gcaatgcggg cgcacagcgc
      181 gcaggatatg ccgtgcgcgc gggcgtgaaa tggcccggcg gcccgcgggg cggcaggcgc
      241 ctcccctggc gaacgcctcc cccctggcga aagagaaaag attgttttgg gacgggcgcg
      301 ccgcgcccgt cccccgcttt gcgcggcgtg tccgccgcgg gaaaggagcg cgcatgcaaa
      361 aacaggacca ggacgtgatc cgggtgaccg gcgccaccga aaacaacctc aagggggtat
      421 cgctggagat ccccaagcac aagatcacgg tctttaccgg cgtttcgggc tccggcaagt
      481 cgtcgctggt gctggacacc atagcggccc agtcccggcg ggagctgaac gagaccttcc
      541 ccagctttgt gcagcactat ctgcccaaat atggccgccc gcatgtggag cgcgttgaaa
      601 acctgcccgc cgccatcgtc atcgaccagc gcaagcccgc gccgaacgcc cgctccaccg
      661 tgggcaccta caccgatatc tacacgctgc tgcggctgct cttttcccgg gtgggcacgc
      721 cctttgtggg ctactccgac gccttttcgt tcaaccaccc ccaggggcgg tgcccgcgct
      781 gcgacggcct gggcgaggtg gaagagctgg acgtgcacaa gctggtggat ttcgacaaga
      841 gcctggacga ggccggctgc atccattata tcgcctttga cccgggccaa tggcgctggg
      901 tgcgctacgc caacagcggg ctgttcgacc tggacaaaaa gatccgcgac tacacgcccg
      961 aggagctgga gctctttctc tacaccccgc ccatcaagct gaaagacccg ccgccgggct
     1021 ggcccaaaac ggccaagtat gagggcttgg tgcaccgcat gtaccgcagc atcatccgct
     1081 cggaggaggg caagctccac cgcaaactgc tggagcccat ggtcgcctcc ggcgtctgcc
     1141 cggactgcgg cggcgcgcgg ctgaaccaaa aggtgctcgc ctgccgcatc gggggcaaaa
     1201 atatcgccga tgccgccgcc acgccgctgc gggagctgcg ggcctgggcg gcctcgatca
     1261 gcgacccttt ggcggaggac ctgaaaaaac tgctggcgga gcggctgtcg gccctcatcg
     1321 agatcgggct ggactacctc acgctggacc ggggcatggg caccctgtcc ggcggggagg
     1381 cacagcgctg caaaatcgca aagtacaaca actcggccct gtcggacctg ctctatattc
     1441 tcgacgagcc cagcgtgggc ctgcacagcc acgacatcca tctgctgcgc tcggcggtgg
     1501 agcggctgcg ggacggcggc aacaccgtgc tgctggtgga gcatcaccgg gagatgatcc
     1561 gggtggcgga ccacctggtg gacatgggcc ccggggccgg cggcgccggg ggagagattc
     1621 tctttgaggg cggttacgag gagctgctgc acggcggcac cgccctcacc ggccgcctgc
     1681 tgcgggcgca t
//
LOCUS       DBMDYA010000650         1689 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14145_length_1689_cov_1.404282, whole genome shotgun sequence.
ACCESSION   DBMDYA010000650 DBMDYA010000000
VERSION     DBMDYA010000650.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_1689_cov_1.404282"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="ACZPFK_12945"
     CDS             <1..678
                     /locus_tag="ACZPFK_12945"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1407259.1"
                     /translation="GSYLPTRFDRVPCDGRRINCSAAARAWVAGLLNPGDPEAAIRDA
                     ATLSMPSHPNAIAISGAAVNAALTAAAFLPGPDCGRLYDLALSAADTGYRLGLAAAGT
                     PPVGAKLPQRIELAANLAVRHRRDPERLLREMNDLIGFGAYASEAVPSAVGLVIAAQG
                     RVGEAVRLAANAGNQACCTAAIAAAACAPACPADEALEECFEQVRAVPPFEWEPLWAE
                     LDIFAAL"
     gene            692..>1689
                     /locus_tag="ACZPFK_12950"
     CDS             692..>1689
                     /locus_tag="ACZPFK_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1407260.1"
                     /translation="MYSLYDRVLGCLVTAGMGDGIGAPTEAMSRQEILEKYGRITGFV
                     DGSTNLVAWGNRTAEITDDTSQMYEMVKAVIRCGGELTAQEAGQALVRWSENYPRYFP
                     RNAGPTTRNVIERLKAGEDPCAVGRTGGRYELGTSNGAIMRIAGAGLCSPGDLEGAIK
                     TAVAMTECSHATQIAFSAACAIACGIAQAVTSRCDVHSVVKACVYGAKRGEEIGLQKA
                     RIAAGARVLPKILRAVELAYLADGPEEAEVLLNDELGTDSSAIAQTCALAIGLFVAAD
                     GDSAGSILGGANIGGDTDTIACISGMLAGARNGYSALPEAWRALFRQANPDLDLCGT"
BASE COUNT          263 a          589 c          588 g          249 t
ORIGIN      
        1 ggctcttacc tccccacccg gtttgaccgc gtcccctgcg acgggcgcag gatcaactgc
       61 agcgccgccg cccgcgcctg ggtggccggc ctcttgaatc ccggcgaccc cgaggccgcc
      121 atccgggacg ctgcgaccct ctccatgccc agccacccca acgccatcgc catctccggc
      181 gcagcggtca acgcggcttt gaccgccgca gcctttctgc ccgggccgga ttgcgggcgg
      241 ctgtacgacc tggctctgag cgccgccgac accggctacc ggctggggct ggccgccgcc
      301 gggacgcccc ccgtgggcgc caaactgccg cagcgaatcg agctggcggc caatctggcg
      361 gtgcggcacc ggcgcgaccc ggaacggctg ctgcgggaga tgaacgacct catcggcttc
      421 ggcgcctatg ccagcgaggc ggtgcccagc gcggtgggcc tcgtgatcgc cgcccagggc
      481 cgtgtggggg aagcggtgcg cctcgcggcc aacgcgggca accaggcctg ttgcaccgcg
      541 gccatcgccg cggcggcctg cgcccccgct tgccctgcgg acgaggcgct ggaggaatgc
      601 tttgagcagg ttcgggcggt tccccccttt gaatgggagc cgctctgggc cgaactggac
      661 atttttgccg cgctttagaa aggaggacgg gatgtattca ctttatgatc gagttctggg
      721 ctgcttggtg accgccggga tgggggacgg catcggcgcg cccactgagg cgatgagcag
      781 acaggaaatc cttgaaaaat acggccggat caccggcttt gtagacgggt ccaccaatct
      841 ggtggcctgg gggaaccgga cggcggaaat caccgacgac acctcgcaga tgtacgagat
      901 ggtcaaagcc gtgatccgct gcgggggtga attgaccgcg caggaggccg gacaggcgct
      961 cgtgcgctgg tcggaaaact atccccgcta tttcccccgc aacgcgggcc cgacgacccg
     1021 caacgtgatc gagcgcctca aggcgggcga agacccctgc gccgtgggcc gcaccggcgg
     1081 ccggtatgag ctgggcacct ccaacggcgc gatcatgcgc atcgcgggcg ccgggctctg
     1141 cagccccgga gatcttgagg gggcgataaa aacggcggtg gccatgacgg agtgctccca
     1201 cgcaacccag atcgcctttt ccgccgcctg cgccatcgcc tgtgggatcg cgcaggcggt
     1261 gaccagcaga tgcgacgtcc actctgtggt gaaagcctgt gtctacggcg ccaagcgggg
     1321 ggaggagatc gggctgcaaa aagcgcgcat tgccgccggg gcgcgggtgc tgcccaagat
     1381 cctgcgcgcg gtggagctgg cctatctggc cgacggcccc gaggaggccg aggtgctgct
     1441 caacgacgag ctgggcaccg actcctccgc catcgcccag acctgcgcgc tcgccatcgg
     1501 cctttttgtg gctgccgacg gcgacagcgc gggctccatc ctgggcggcg cgaacatcgg
     1561 cggggacacc gacaccatcg cctgtatctc ggggatgctc gccggggcgc gcaacggcta
     1621 cagcgccctg cccgaggcct ggcgcgcgct gttccgccag gccaaccccg acctggacct
     1681 ctgcggcac
//
LOCUS       DBMDYA010000651         1677 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14261_length_1677_cov_1.442259, whole genome shotgun sequence.
ACCESSION   DBMDYA010000651 DBMDYA010000000
VERSION     DBMDYA010000651.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14261_length_1677_cov_1.442259"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="ACZPFK_12955"
     CDS             complement(<1..234)
                     /locus_tag="ACZPFK_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR1407261.1"
                     /translation="MCCYMACVIVIGLVFARRANQSTENYFLGGRSLGPWIAAMSAEA
                     SEMSGWLLMGLPGVAYWCGVADAAWTAIGLGVGT"
     gene            complement(361..747)
                     /locus_tag="ACZPFK_12960"
     CDS             complement(361..747)
                     /locus_tag="ACZPFK_12960"
                     /inference="COORDINATES: protein motif:HMM:NF016152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR1407262.1"
                     /translation="MLTEQRLVEYALTFPDVYRDMPFHDDTVYLRCRGNKKGFCMLLH
                     RAGRLYVNLKCSPQEAEFYRRVYRDVIPGWHMNKTHWNTVFLNGDVPEEEVLRMLRSA
                     HALVAPRRRKREGEAAKSCQKAENQV"
     gene            complement(1300..>1677)
                     /locus_tag="ACZPFK_12965"
     CDS             complement(1300..>1677)
                     /locus_tag="ACZPFK_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014689.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HIR1407263.1"
                     /translation="GKSHAAPALRRHGGRGSVRFTALGLQFLLAVYACPEEADADHSK
                     RLGALEAVSLGVGLSVDSLAAGLGAGLGSVAILPTLAASLLLGMGMLAAGSACARWAA
                     SRLPVDLSPVGGVLLIALAVLRL"
BASE COUNT          330 a          598 c          442 g          307 t
ORIGIN      
        1 agtgcccacg cccaaaccga tggcggtcca ggcagcgtcg gccacgccgc accaataggc
       61 cacgcccggc aggcccatca gcagccagcc gctcatctcc gaggcctcgg cgctcatggc
      121 ggcgatccac gggcccaggc tgcgcccgcc gagaaaatag ttttccgtgc tctggttcgc
      181 cctgcgggca aaaaccagcc cgatgacgat gacacaggcc atataacagc acatggccac
      241 caatgtctgt acagttttaa agtccacccc ttgtttcctc cctgtgttcc agattgcctt
      301 gtccgcaggc cggcgaggac gcgccgctgc gcctcgccac tgaaaaaatg atatgttcca
      361 tcatacctga ttttcagctt tttggcaact ttttgccgct tcgccctctc ttttgcgccg
      421 ccgcggcgcc accagggcgt gcgccgaccg cagcatgcgc agcacctcct cctccggcac
      481 gtccccattg agaaagaccg tgttccaatg ggttttgttc atgtgccagc cggggatcac
      541 atcccggtac acgcgccggt aaaactccgc ttcctggggg ctgcatttca ggttcacata
      601 taaccgcccg gcccggtgca gcaacatgca gaaccccttt ttgttgcccc ggcagcgcag
      661 ataaaccgtg tcgtcgtgga aaggcatgtc ccgatagaca tcgggaaagg tgagcgcata
      721 ctccacaagc cgctgttccg tcagcatttt ttgcatcctc tctttttcat cgtgtgccaa
      781 ggtctctcag cgccggccgc aaaaacggcg gtccgggggg aggccgcctt gagggggcct
      841 ctccggaccg ccgagcccct tttctactgc cgcgacctaa aaacaaccta tctcaatggc
      901 tccgtttctc gccgctgcgc ggcaaccgaa acggatggcc gaaaaaaaac agcctccgct
      961 tttctgcggc tatttttata gctttgcaac acaaagccat aataacggcc cacggcttta
     1021 aaatctcaag cgcccgccca cttccgtggg cggatgcgcg taaaaaacaa cttatttcta
     1081 aggccccggc ccgaggacgg gccacatggc cgaaaaaaca gtctccgctt cgccgcggct
     1141 attttttatt gcttcgcaat aaaaaactta tcataatggc cccgtttctc gccgctgcgc
     1201 ggcaaccgaa acggacggcc caaaaaatag cctgcggcta ttttttattg taccacattt
     1261 tgccacaacc tcgcacgctt gcccgcccgg agcgccggct cacagccgca gcaccgccag
     1321 cgcgatcagc agcacgcccc ccaccgggga cagatccacc ggcaggcggg acgccgccca
     1381 gcgggcgcag gcgctgcccg ccgcgagcat ccccatgccc agcagcaggc tggccgccag
     1441 ggtgggcaaa atcgccacgc tgcccagccc ggcccccagc cccgcggcca ggctgtccac
     1501 cgacagcccc acccccaggc tcacggcctc cagcgcgccc agccgtttgc tgtggtcggc
     1561 gtcggcctcc tcggggcagg cgtatacggc cagtaaaaac tgcagcccca gcgcggtaaa
     1621 tcgcacgctg cccctgcccc cgtgccgccg cagggcaggg gcagcgtgcg atttacc
//
LOCUS       DBMDYA010000652         1676 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14277_length_1676_cov_1.278730, whole genome shotgun sequence.
ACCESSION   DBMDYA010000652 DBMDYA010000000
VERSION     DBMDYA010000652.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14277_length_1676_cov_1.278730"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="ACZPFK_12970"
     CDS             <1..197
                     /locus_tag="ACZPFK_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407264.1"
                     /translation="FPEELALAAAAVVVLVLFALRQARVADGLVNFFQKLRSLRRPAA
                     SADGKKAEDAGQNGPDGEVG"
     gene            199..1293
                     /locus_tag="ACZPFK_12975"
     CDS             199..1293
                     /locus_tag="ACZPFK_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1407265.1"
                     /translation="MTTQPNLFLSAVGAVFSNGYLASVLLVGTFLMFTVIGVIFARRS
                     GVFFLAAEGVLVFSATAGYFANAATRAALLGSAAEGSAAYEAALGRADVLGWVAGLFC
                     GLAGGMAFTLLFCWFLLRVKSNALLTSFALNVFASASAGVLMHLVNRGDGAVSSAVAA
                     ALPSLKLSALAEVPVLGGLVGDTGWFTYLALLLPPAAFYLLNRTGFGLRLRAAEENPI
                     ALTATGLDVSRTQLYGMLFAGAAVSLGGVTLVMSGPAPVFSALPHGEGYLALAAVWAC
                     DGRLWKSSVAVLLLALVGALARVLHELPLPVSLIQALFYAAALAGLLLHSHNDKHRHK
                     VSLRAQRRMVEGKEEVKKKRERKKRPEKKK"
     gene            complement(1308..>1676)
                     /locus_tag="ACZPFK_12980"
     CDS             complement(1308..>1676)
                     /locus_tag="ACZPFK_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407266.1"
                     /translation="RFFSLFSGQERQSFSLWGVPALHLGCVDRAPLYLFPGSGGIFFS
                     ALAAEFLFCLAWNSAVCRPARSTGKKRREIKRAGRPNAEVHRAAAPSIPPFQPRKRLP
                     ARFFAGGKLFWLYHVLPRRA"
BASE COUNT          281 a          506 c          566 g          323 t
ORIGIN      
        1 gcttcccgga agagctggcc ctggcggccg cggcggtggt ggtgctggtt ctgtttgcgc
       61 tgcggcaggc gcgggtcgcc gatggtttgg tgaacttttt tcaaaagctg cgcagcttgc
      121 gacggcccgc ggcctctgcc gacggcaaaa aggccgaaga cgcggggcaa aacggcccgg
      181 atggggaggt agggtaatat gacgacacag ccgaaccttt tcctctcagc cgtcggcgcc
      241 gttttcagca acggctatct cgcctcggtg ctgctggtgg gcaccttttt gatgtttacc
      301 gtgatcggcg tcatcttcgc ccggcgcagc ggcgtctttt ttctggcggc cgagggggtg
      361 ctggttttca gcgccacggc gggctatttt gccaacgccg cgacgcgcgc cgccctgttg
      421 ggctccgccg ccgagggcag cgccgcctat gaggccgcgc tggggcgggc cgatgtgctg
      481 ggctgggtgg ccggcctgtt ttgcgggctt gccggcggta tggctttcac gcttttgttc
      541 tgctggtttt tgctgcgggt caagagcaac gccctgctca ccagctttgc gctgaacgtt
      601 tttgcgtcgg caagcgccgg cgtcttgatg caccttgtaa accgcgggga cggtgcggtc
      661 tcctccgccg tcgccgccgc gctgccctcc ttgaagctct ccgccctggc ggaggttccc
      721 gtgctgggcg gcctggtggg ggataccggc tggttcacct atctggccct gctgctgccc
      781 ccagctgcct tttacctgct caaccgcacc ggctttgggc tgcggctgcg ggcggctgag
      841 gagaacccca tcgccctgac cgccactggc ctggacgtga gccgtacgca gctgtacggc
      901 atgctgtttg cgggcgcggc ggtgagcctg ggcggcgtga ccctggtcat gtccggcccg
      961 gccccggtat tttccgccct gccccacggc gagggatatc tggccctggc ggcggtatgg
     1021 gcctgcgacg ggcggctctg gaagagcagt gtcgccgtgc tgctgctcgc cctggtcggg
     1081 gcgctggccc gtgtcctgca cgagttgccc ctgcccgtct ctctcatcca ggctctgttt
     1141 tacgcggcgg cgctggcagg gctgctgctc cattcgcaca acgacaagca ccggcataag
     1201 gtgagcctgc gcgcacagcg ccgcatggtg gaggggaaag aggaagtcaa aaaaaagcgg
     1261 gagcgcaaaa aacgtcctga aaagaaaaaa tagggcgttg tttcggctca ggcgcgcctc
     1321 ggcaagacat gatacaacca aaaaagcttc ccaccggcga aaaaccgggc gggaagcctt
     1381 tttcgtggct gaaaaggggg aatcgacggc gccgctgcgc gatggacctc ggcattcggg
     1441 cggcctgcgc gctttatttc tcggcgcttt tttccggtgg agcgggcggg acgacaaact
     1501 gcagaattcc acgccaggca aaagaggaat tcagcagcca gagcgctgaa aaatatccca
     1561 ccggaaccag gaaaaaggta taggggagcg cgatccacac agccaaggtg cagagcaggg
     1621 acgccccaca gagaaaaaga ctgtctttcc tgtccagaaa aaagagaaaa aaagcg
//
LOCUS       DBMDYA010000653         1676 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14279_length_1676_cov_1.177143, whole genome shotgun sequence.
ACCESSION   DBMDYA010000653 DBMDYA010000000
VERSION     DBMDYA010000653.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14279_length_1676_cov_1.177143"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(141..824)
                     /locus_tag="ACZPFK_12985"
     CDS             complement(141..824)
                     /locus_tag="ACZPFK_12985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1407267.1"
                     /translation="MIRSVCIFGDSVARGVVLDEAKQRYVDSRSCFARLVAEASHIAV
                     ENFARFGCTVTKGSRVAQRHLEDAARADYTVVEFGGNDCDYRWQQVAAAPLAPHDCNT
                     PETRFCAEYAALLRQVRQAGGRPVAFSLPPVEEERYFDWISRGLSKDALLQFLGGVRR
                     IYDWQAHYSDLAVQTAQALGAPVVDLRGAFLSQRDYRDLLCRDGIHPNEAGHRLITQV
                     LEDRIEALA"
     gene            977..1540
                     /locus_tag="ACZPFK_12990"
     CDS             977..1540
                     /locus_tag="ACZPFK_12990"
                     /inference="COORDINATES: protein motif:HMM:NF020450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1407268.1"
                     /translation="MKETQELFGRLSEWRPAPYASLPDLDLYKDQVLSYMQRQQLPGE
                     SADELTGAMINNYIKSGVLPRPAGKKYQRQHLAALTAICQLKQVLSVSDTSALLACQP
                     DASDTEAFYGRYLEQLDEALRRAAETLPKAGEDSREALARCALTLAVQSYADRLAARE
                     LLALLEKAGRADTGAPEGERTAQGPKK"
BASE COUNT          302 a          553 c          548 g          273 t
ORIGIN      
        1 ccccgctctc ttctgttttg ccgaacgccc gccgggggcc gcctctgccg cgcgggcacg
       61 cggccttttt tgcggccggg ccgggcgttc gttcagggcg tgagggcggc cgtgtcggct
      121 cttttccccg ggcgggccgg tcaggccagc gcctcgatgc ggtcctccag cacctgggtg
      181 atcagccggt gcccggcctc gttggggtgg atgccgtccc ggcagagcag atcccggtaa
      241 tcgcgctgcg agaggaacgc cccgcgcagg tccaccaccg gcgcccccag cgcctgcgcc
      301 gtctgcaccg ccaggtcgct gtagtgggcc tgccagtcgt agatgcggcg gacgccgccc
      361 aaaaattgca gcagcgcgtc tttggagagg ccgcgggaga tccagtcgaa atagcgctct
      421 tcctccaccg gcggcaggct gaacgccacc ggcctgccgc ccgcctggcg cacctgccgc
      481 agcagggcgg catattccgc gcaaaagcgc gtttccggcg tgttacagtc atggggggcc
      541 agcggggcgg cggccacctg ctgccagcgg taatcacagt cattgccgcc gaactccacc
      601 accgtgtagt cggcccgggc ggcgtcctcc agatgccgct gcgccacccg gctgcccttg
      661 gtgacggtgc agccgaagcg ggcaaaattt tccaccgcga tatggcttgc ctcggccacc
      721 agccgggcaa agcaggacct gctgtccaca tagcgctgct tggcctcgtc cagcaccacg
      781 ccccgggcga ccgagtcccc aaaaatacaa acggaacgga tcatggttca ttacctcacg
      841 aatataatat taaatattat attatatgat tattattatg tcaattaagc gaccttgtta
      901 ttcaaactat ttacaatttt taccgtcttt gataaaatag agctgatctc aatcgcccca
      961 aagggaggga aaagacgtga aagagacaca agagctgttc ggccgcctgt cggagtggcg
     1021 ccccgcgccc tacgccagcc tgccggacct ggatctctac aaagaccagg tgctcagcta
     1081 tatgcagcgc cagcagttgc ccggtgagag cgcggacgag ctcaccggcg ccatgatcaa
     1141 caactatatc aagagcgggg tgctgccgcg cccggcgggc aaaaaatacc agcgccagca
     1201 tctggcggcg ctgacggcca tctgccagct caagcaggtg ctctcggtgt cggacaccag
     1261 cgccctgctg gcctgccagc ccgacgcgtc ggacaccgag gccttttacg gtcgctatct
     1321 ggagcagttg gacgaggcgc tgcgccgcgc ggccgagacc ttgcccaaag ccggggagga
     1381 cagccgtgag gcgctggcgc gctgtgccct caccctggcg gtgcagagct atgccgaccg
     1441 cctggccgcc cgcgagctgc tggccctgct ggagaaagcc gggcgggctg atacgggcgc
     1501 gcccgagggg gaaaggacgg cacaggggcc gaaaaagtga atcgccgccg cagggcgtag
     1561 cggggcccgg cccgcgcggg cgggcaaaag ggtgttttca gcccgaagcg ctgcatgtcg
     1621 cctggcagcc cgaaagccgg ggccgtgagg tgcgggcagg tgagaagggc cgccct
//
LOCUS       DBMDYA010000654         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14290_length_1675_cov_1.235705, whole genome shotgun sequence.
ACCESSION   DBMDYA010000654 DBMDYA010000000
VERSION     DBMDYA010000654.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14290_length_1675_cov_1.235705"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            166..>1675
                     /locus_tag="ACZPFK_12995"
     CDS             166..>1675
                     /locus_tag="ACZPFK_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1407269.1"
                     /translation="MKTTLCQVNSQYIHSALAPWCLAAAARQRCADPGELRVVDANIN
                     QPLRQVACRLAEGQPGLLALSCYIWNAAYLHALLPLLRALLPEAVIAVGGPEASFDTA
                     AFLRANPAADCVLVGEGEASFPLFLDALASGAAWAEVPGLVYRDGAKIKSNPPPPPVA
                     LPPDPYTPDYFAALKGRIAYVETSRGCPFTCAFCLSGREDRLRQFPLPEMERHLMKLA
                     SSGCGTVKFVDRTFNADEKRAVELWRFLIRQRLAGGIPAGVCFHFEVGADLFSDACLA
                     FLKTAPPGLFQFEAGLQSFNPETLGAVQRKTDLDRLCANLLSIRAGGNIHLHVDLIAG
                     LPYEDEASFAASFDRAFALGPHQLQLGFLKLLRGSALRARAGELGLQYDPAPPYEVRA
                     TRWLWPAALDRLKTAERALGWLYNSGRYPRALPELLERWQGGAFAFFAAFGSFAAAQG
                     AGEHPAQDAVAELLFLFGGSLAGMSPRRLRDLLCLDLLSSRRGGRLPGFLRVA"
BASE COUNT          201 a          590 c          591 g          293 t
ORIGIN      
        1 cggcggcgtg tccgcgccgg aagcggagct gatccgcagc gtggcgctcg cctgcctggg
       61 actgcagctg gtaaccctga tcgccggcgc ggccgcgctg gtctgggtgc tgctgctcaa
      121 ccaaaaacgc cgcgaggccg tctcgctgcg cccctgtagg ctgccgtgaa aacgactctc
      181 tgtcaggtga acagccagta catccactcc gccctggcgc cctggtgcct tgccgccgcg
      241 gcgcggcagc gctgcgccga cccgggcgag ctgcgggtgg tggacgccaa catcaaccag
      301 cccctgcggc aggtggcctg ccgcctcgct gaggggcagc ccggcctgct ggcgctgagc
      361 tgctatatct ggaacgccgc ctatctgcac gcgctgctgc cgctgctgcg cgcactgctg
      421 ccggaggccg tgatcgcggt cggcgggccg gaggccagct ttgacaccgc ggcctttctg
      481 cgggccaacc cggccgccga ctgcgtgctc gtcggcgagg gggaggcctc ttttccgctt
      541 tttcttgacg ctctggcctc aggcgctgcg tgggcggagg tgccgggcct ggtctaccgc
      601 gatggggcaa agatcaaaag caacccgccg cccccgccgg tggcgctgcc ccccgacccc
      661 tacacgccgg attattttgc cgcgctgaaa gggcgcatcg cctatgtgga gaccagccgg
      721 ggctgccctt tcacctgcgc tttctgcctg tcgggacggg aggaccgtct gcggcagttt
      781 ccgctgccgg agatggagcg ccacctgatg aagctggcct cctcggggtg cggcaccgtc
      841 aaatttgtcg accggacgtt caacgccgac gaaaaacgcg ccgtggagct ctggcgcttc
      901 ctcatccggc agcggctggc cggcggcatc cccgccgggg tctgttttca cttcgaggtg
      961 ggggcggacc tgttcagcga cgcctgcctc gcctttttga agaccgcgcc gccgggcctg
     1021 ttccagtttg aggccgggct gcagagcttc aaccccgaga cgctgggggc cgtgcagcgc
     1081 aaaaccgatc tcgaccgcct ctgcgccaac ctgctctcga tccgggcggg gggcaacatc
     1141 catctgcatg tggacctgat cgccgggctg ccctacgagg acgaggcctc ctttgcggcc
     1201 agcttcgatc gcgcgttcgc gctggggccg caccagctgc agctcggctt tttgaagctg
     1261 ctccggggca gcgcgctgcg ggcgcgggcc ggggagctgg ggctgcagta cgacccggcg
     1321 ccgccctatg aggtgcgggc cacccgctgg ctgtggcccg cggcgctgga ccgcctcaaa
     1381 acggccgagc gggcgttggg ctggctctac aacagcggcc gctatccccg cgccctgccc
     1441 gagctgctgg agcgctggca gggcggtgcc tttgcctttt tcgccgcttt cggcagcttt
     1501 gcagcggccc agggcgccgg ggagcacccg gcgcaggacg cggtggcgga gctgctcttc
     1561 ctctttgggg gcagtctggc gggcatgtcc ccgcgccgtc tgcgggacct gctctgcctg
     1621 gacctgctct cgagccgccg cggcggccgt ctgcccggtt ttttgcgggt ggcgg
//
LOCUS       DBMDYA010000655         1667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14388_length_1667_cov_1.743295, whole genome shotgun sequence.
ACCESSION   DBMDYA010000655 DBMDYA010000000
VERSION     DBMDYA010000655.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14388_length_1667_cov_1.743295"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(89..991)
                     /locus_tag="ACZPFK_13000"
     CDS             complement(89..991)
                     /locus_tag="ACZPFK_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006873 - intracellular monoatomic ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR1407270.1"
                     /translation="MSASDTKRPLSAQTLKLLKKMQVGELTEHLIYTRVADIVEKKSP
                     ENAAVLRRVGEEERRHAAIWRAYTGFEAKPDLHKVRRTVLLARVFGFTFAMKKMESGE
                     ESAQKTYGRLFAEVPEAERICADEERHEQELLAMLDEERLHYVGSMVLGLNDALVELT
                     GTLAGLTFAMQNNKLVALSGLITGASATLSMASSEYLSARSEGRQDALKSCVYTGIAY
                     LVTVTLLVLPYLLLPAGAYLVSLLIMLLTVVLIIAGFTYYVSVAQGLAFRRRFTEMAA
                     ISLGVAVISFVIGLIVKQCLGIDV"
     gene            complement(1053..1643)
                     /gene="pth"
                     /locus_tag="ACZPFK_13005"
     CDS             complement(1053..1643)
                     /gene="pth"
                     /locus_tag="ACZPFK_13005"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1407271.1"
                     /translation="MFFKKPSPIDFLIVGLGNPGRQYEGSHHNAGFLALDHLAKELGC
                     EVARAKFQALTGQAALGGRKVLLMKPQTFMNLSGNAVGAAAKFYKLAPAQVLVLYDDI
                     ALAPGKLRIRPSGSAGGHNGIKSIIAALGSQEFPRIRIGVGERRGGEADLADFVLSKF
                     SAEDKKAVFARFDDICAAAGLIVDGDLASAMNRYNH"
BASE COUNT          294 a          529 c          547 g          297 t
ORIGIN      
        1 gctgatattc ttttgtctgt cgcagcgctt cttcaagcat aacggtttta tttcctcatg
       61 gggtggatgg ggcgcggcaa agggcggctc agacgtcgat gcccaggcac tgcttgacaa
      121 tgagcccgat gacgaaagag atcaccgcca cgcccaggct gatggctgcc atctcggtga
      181 agcggcggcg gaacgccagc ccctgggcca ccgagacgta ataggtgaag ccggcgatga
      241 tgagcaccac cgtcagcagc atgatgagca gcgagaccag ataggcgccc gcgggcagca
      301 gcagataggg cagcaccagc agcgtcacgg tgacgagata ggcgatgccg gtgtacacac
      361 aggatttcag cgcgtcctgc cgcccctcgc tgcgggcgga gagatattcg ctggaggcca
      421 tcgagagggt ggccgaggcg ccggtgatca ggcccgagag ggcgaccagc ttgttgttct
      481 gcatggcgaa agtgaggccc gccagcgtgc cggtcagctc caccagggcg tcgttgagcc
      541 ccagcaccat gctgcccaca tagtgcagcc gctcctcgtc cagcatggcc agcagctcct
      601 gctcatgccg ttcctcgtcg gcgcagatgc gctccgcctc cggtacctcg gcgaacaggc
      661 ggccgtaggt cttctgcgcg ctctcctcgc cgctctccat ctttttcatg gcaaaggtga
      721 agccgaagac ccgggccagc agcacggtgc ggcgcacctt gtgcaggtcc ggctttgcct
      781 caaagccggt gtaggcccgc cagatggcgg cgtgacggcg ctcctcctcg cccacccggc
      841 gcagcacggc cgcgttttcg ggcgactttt tctccacaat gtcggccacc cgggtataga
      901 taaggtgctc ggtgagctcg cccacctgca tctttttgag cagcttcagc gtctgggcgg
      961 agaggggcct tttggtatcg gaagcggaca tggcaactac ctccttgaaa gagatcacgg
     1021 cgcggcccgg gcgtttgccg gggcgcggcc gctcagtggt tgtaccggtt catcgcggag
     1081 gccaggtccc cgtctacgat aagcccggcc gcggcgcaga tgtcgtcgaa gcgcgcgaag
     1141 acggcttttt tgtcctcggc gctgaatttg gaaagcacga aatcagccag gtcggcctcg
     1201 ccgccccgtc gctcgcccac cccgatgcgg atgcggggga actcctgtga gcccagcgcg
     1261 gcgatgatgc tcttgatgcc gttgtgcccg cccgcggagc cggagggccg gatgcgcagc
     1321 ttgcccggcg ccagggcgat gtcgtcgtag agcaccagca cctgcgccgg cgccagcttg
     1381 taaaatttgg ccgccgcgcc caccgcgttg cccgacaggt tcataaaggt ctgcggcttc
     1441 atcagcagca ccttgcggcc gcccagcgcg gcctggccgg tgagagcctg gaatttggcg
     1501 cgggccacct cgcagcccag ctccttggcc agatggtcca gggccagaaa gccggcgttg
     1561 tggtgcgagc cctcgtactg gcggccgggg ttacccaggc cgacgataag aaaatcaatg
     1621 ggagacggtt ttttgaaaaa cataatacct cttgtcagac aggggac
//
LOCUS       DBMDYA010000656         1667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14389_length_1667_cov_1.310345, whole genome shotgun sequence.
ACCESSION   DBMDYA010000656 DBMDYA010000000
VERSION     DBMDYA010000656.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14389_length_1667_cov_1.310345"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            288..1115
                     /locus_tag="ACZPFK_13010"
     CDS             288..1115
                     /locus_tag="ACZPFK_13010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1407272.1"
                     /translation="MDAWVKTGEEAEKDGQQAPEQKHIFRAGGKAGKPGEDKVSPILT
                     VQLLFCLCVVAFVLVMRFASPGFFAQMGERYEKMLTEGVSLTSSGELFRFASAGVESL
                     QEKLRRAVEQLGAKEPDSALGQGGQYEVTHAGYLKTVTLASYVLSDRPGMPVEGTLTS
                     PFGYRTHPITGKTDFHTGVDLAAPQGTPVAAAWMGVVAETGFNDINGNYVKMIHSGNV
                     CTTYNHLSAISVQTGQRLRRGEVVGLVGSTGISTGPHLHFELLIDGVRVDPAPALGL"
     gene            1112..>1667
                     /locus_tag="ACZPFK_13015"
     CDS             1112..>1667
                     /locus_tag="ACZPFK_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014244.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1407273.1"
                     /translation="MKRFFSVRAPVPLLAALTLLLLWDATGFAKLSLLCAALHECGHI
                     LAFCALTGHFPRITCSFSGLAMEMDGVRLSLQKENLLLLAGPAANLLAALAAYLLIQH
                     RASYLRYFFACENLCMALFNLLPIGFLDGGRLLSNCFGLMDGVLFRALTLCCCFFLAV
                     FGGVLLRYCGAGFLLSLGFCLMVLA"
BASE COUNT          279 a          488 c          536 g          364 t
ORIGIN      
        1 ggcgtctggg cgggcggcga cgcggtgacc ggcgccgcca ccgtcatctc cgccatgggc
       61 gcgggcaaaa aagccgccgc cgccatctcc gcgtatctgg cggaaaaata gggcggcctt
      121 atccggcttg cggccgatac cctcccaata aaaacttatt cgatttttaa aaagaatcaa
      181 aagaaccatc tgtaacgctg ctttgcttta cagatggttc ttttcttttt tgccgctgca
      241 taaactgcat ggaatggaga tcaataggcg aggaggtgcc tggcaaaatg gatgcatggg
      301 taaagaccgg cgaggaagcg gaaaaagacg gccagcaggc cccggagcaa aaacatattt
      361 tccgcgcggg cggcaaggcg ggcaagccgg gggaggacaa ggtcagcccc atcctgacgg
      421 tgcagctgct gttttgcctt tgcgtggtcg cgtttgtgct ggtgatgcgg tttgcctctc
      481 ctggcttttt tgcgcagatg ggagagcggt atgaaaaaat gctcaccgag ggcgtgtccc
      541 tcaccagcag tggagagctg ttccgctttg ccagcgccgg cgtcgagagc ctgcaggaaa
      601 agctgcgccg cgccgttgaa cagctggggg cgaaagagcc ggacagtgcg ctggggcagg
      661 ggggccagta cgaggtcacc catgccggat atctgaaaac ggtgacgctg gcgagctatg
      721 tgctcagcga ccggcccggc atgccggtgg aggggacgct gacctccccg tttggctacc
      781 gcacccatcc catcaccgga aagactgatt ttcacaccgg cgtggacctg gccgcgccgc
      841 aggggacgcc ggtggccgcc gcctggatgg gggtggtggc ggagaccggc ttcaacgaca
      901 tcaacggcaa ctatgtgaaa atgatccatt ccggcaatgt ctgcaccacc tataaccatc
      961 tgtcggcgat ctcggtgcag acgggccagc gcctcaggcg gggcgaagtc gtggggcttg
     1021 tgggcagcac cggcatctcc accgggccgc atctgcactt tgaactgctg atcgacgggg
     1081 tgcgggtaga cccggccccg gcgctggggc tttgaaacgc tttttttcgg tccgggcgcc
     1141 ggtgccgctg ctggcggctt tgaccctgct gctgctctgg gacgccaccg gctttgccaa
     1201 gctgtcgctg ctctgcgccg cgttgcacga atgcggccat atcctggctt tctgtgccct
     1261 caccgggcat tttccccgca ttacctgctc tttttccggc cttgcgatgg agatggacgg
     1321 ggtgcggctt tcgctgcaaa aagaaaacct gctgctgctt gcgggccctg cggcaaacct
     1381 gctggccgcg ctggccgcct atctgctgat ccagcatcgc gcctcttatc tgcgctattt
     1441 tttcgcctgc gaaaacctgt gtatggctct tttcaacctg ctgcccatcg gctttctgga
     1501 cggcgggcgg ctgctctcca actgctttgg gctgatggac ggcgtgctgt tccgggcgct
     1561 gaccctgtgc tgctgcttct tcttggctgt tttcggcggt gtgctgctgc ggtactgcgg
     1621 cgccggtttt ttgttatcac tgggcttttg cctgatggtg ctggccc
//
LOCUS       DBMDYA010000657         1651 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14570_length_1651_cov_1.494194, whole genome shotgun sequence.
ACCESSION   DBMDYA010000657 DBMDYA010000000
VERSION     DBMDYA010000657.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14570_length_1651_cov_1.494194"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="ACZPFK_13020"
     CDS             <1..723
                     /locus_tag="ACZPFK_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014926.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit-related
                     protein"
                     /protein_id="HIR1407274.1"
                     /translation="PLRPQIARAVTDNDRYLGLPGPLRRLLDPGFWLRADRGMRPETS
                     SLTGSGILFSYKARGMKHLSLSYRMARDGRLAVQLVATPRRGGLPRAGVTFAAAPGLR
                     TVRYFGLGPHENYVDRRAAALPGDYVFPAGEFCHDYLHPQENGNRTGVERFRLSGGGH
                     ALEAVTLGAPFEASAHPYTLRQLEAAQHADELDHNGPVTVNLDAAQRGVGGDMPAMAL
                     TKPPYCLHKKETYTLEVLLRGE"
     gene            complement(1040..>1158)
                     /locus_tag="ACZPFK_13025"
     CDS             complement(1040..>1158)
                     /locus_tag="ACZPFK_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HIR1407275.1"
                     /translation="VLFEKAFRCEAEVELLKSIKMGDPVCLMKIILHNPIWQ"
     assembly_gap    1159..1168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1169..1595)
                     /locus_tag="ACZPFK_13030"
     CDS             complement(<1169..1595)
                     /locus_tag="ACZPFK_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HIR1407276.1"
                     /translation="MFDIRKIQEDVIYKAVKAESDEETARKVVYGGQQKKAENNPGWV
                     KAAMHRLENSFDSGTVKQIRRNCQCGYGMEEKLALVKELMVGCTSIEELTSSEKAKAA
                     GLFCKNGELFLQFSFCPCPMLAEVDKLETAAWCQCTTGYS"
BASE COUNT          309 a          521 c          429 g          382 t
ORIGIN      
        1 cccctgcgcc ctcagatcgc ccgggcggtc accgacaacg accgctatct ggggctgccc
       61 ggcccgctgc gccggctgct cgaccccggc ttctggctgc gggccgaccg cgggatgcgc
      121 cctgagacct cctccctgac cggcagcggc atccttttct cctacaaagc gcggggcatg
      181 aagcacctct cgctgagcta ccgcatggcg cgggacgggc ggctggccgt gcagctggtg
      241 gctacgccgc gccggggcgg cctgccccgg gcgggggtca cctttgccgc cgcccccggg
      301 ctgcgcacgg tgcgctattt cggccttggc ccgcacgaaa actacgtcga ccgcagggcc
      361 gccgccctgc cgggggatta tgtctttccc gccggggagt tctgccacga ctacctgcac
      421 ccgcaggaaa acggcaaccg caccggggtg gagcggttcc gcctgtcggg aggcggccac
      481 gcgctggagg cggtgacgct gggcgccccc tttgaggcct ccgcccatcc ctataccctg
      541 cggcagctgg aggcggcgca gcatgcggac gagctggacc acaacggccc ggtgaccgtc
      601 aatctggacg ccgcacagcg gggggtgggc ggcgacatgc cagccatggc cctcacaaag
      661 cccccctatt gcctgcacaa aaaggagacc tatacgctgg aggtgctgct gcggggggaa
      721 tgagccccgg cggagcgaga aaccctctcg ctccgcttgg tcggctcaaa tctattaagt
      781 acatcctgtc cgcccatgac aaccctcttt tttattgctc cgcaataaaa aacaacttat
      841 cttaatggcc ccggccagag gactggccgg atggccgaaa aatagcctcc gctttgctgc
      901 ggttattttt tatagctttg catcgcaaag ctataaaaac ggccatgtgg gccgtcccgg
      961 cccacggcct taaaatcttg agcgccgccc acgggagtgg gcggatgcgc gaaaaaaata
     1021 gccgcaaagc ggctattttt tattgccata tggggttgtg gaggataatt ttcataaggc
     1081 atacaggatc gcccattttt atacttttca gcagttcaac ctcggcttca cacctaaacg
     1141 ctttttcaaa aaggacttnn nnnnnnnntg ctgtaaccag tcgtacactg gcaccaggca
     1201 gccgtctcta atttatccac ttccgcaagc atagggcagg gacaaaaact aaattgcaga
     1261 aagagctcac cgtttttgca aaacaatccg gcagccttgg ctttttctga actcgttaat
     1321 tcttctatac ttgtgcagcc taccatcagc tcctttacaa gcgcaagctt ttcctccatc
     1381 ccgtaaccgc actggcagtt cctccgtatc tgcttcacgg ttccgctatc gaagctgttc
     1441 tccaggcggt gcattgccgc ctttacccat cctggattat tttctgcctt tttctgctgc
     1501 ccgccatata ccacttttct tgcggtttct tcatcgcttt cggctttgac agccttatag
     1561 attacatctt cctgtatttt tcttatatcg aacataattt tctccctttt atcctgtgta
     1621 acattccggt tatctgcatt tatttccttt g
//
LOCUS       DBMDYA010000658         1648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14606_length_1648_cov_1.285068, whole genome shotgun sequence.
ACCESSION   DBMDYA010000658 DBMDYA010000000
VERSION     DBMDYA010000658.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14606_length_1648_cov_1.285068"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="ACZPFK_13035"
     CDS             complement(<1..257)
                     /locus_tag="ACZPFK_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407277.1"
                     /translation="MSLAVLLRSRLSDGAFYLCDQGFRRDFEKLHQVDAHEKARQHLA
                     RLNLLVPPQGNKDLVRNFLLREPLVIAGVKDLMRKNVRHLSL"
     gene            complement(298..570)
                     /locus_tag="ACZPFK_13040"
     CDS             complement(298..570)
                     /locus_tag="ACZPFK_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1407278.1"
                     /translation="MELNGFGIAGVACITVICYLAALGMKQTPLANKWLPTICGALGA
                     VLGPVCMLVVPDFPAGDPVTAVAVGIVSGLAATGADQLAKQLKKTQ"
     gene            complement(571..789)
                     /locus_tag="ACZPFK_13045"
     CDS             complement(571..789)
                     /locus_tag="ACZPFK_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407279.1"
                     /translation="MPQFDTSFWVQILVYAVTFAATWGSVTQRLKMLEQKVDKHNSVV
                     ERMAVAERDLKSAWKQIDEIKEDIKEEK"
     gene            complement(789..>1648)
                     /locus_tag="ACZPFK_13050"
     CDS             complement(789..>1648)
                     /locus_tag="ACZPFK_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1407280.1"
                     /translation="ARRAAREAAALLAPYRLTMPLAFDIEDAATYKALGRAQSNAVAA
                     AFLEEAKVQGYYPLLYTYTSFANSWLDMSALSAYDLWLADYRGYMGLKGASIWQHTSD
                     GRVDGISGRVDLNIAYKDYPALIGRQGGQKEDKNDMMQFLEVFGSKNCQCFTGPDVDA
                     VDRSYNNGTLASGTYYPLMADCGLDADGYRWVRVLAGGAERYAVVLDDRCRITGLSAG
                     DAVRAVQAQGAGGDAAALARRVKELESSNAGLLARASAAEAQSAEAARRAAQYLKRIK
                     AAAAALEVL"
BASE COUNT          269 a          577 c          487 g          315 t
ORIGIN      
        1 agcgaaagat ggcgcacatt tttgcgcatg aggtctttca cgcccgcgat caccaggggc
       61 tcacgcagca agaaattgcg gaccaggtct ttatttccct gcggtggtac caaaagattg
      121 aggcgggcga ggtgctgccg ggctttttcg tgggcgtcca cctgatggag cttttcaaaa
      181 tcgcgccgga aaccctggtc gcagagataa aaagcgccat cgctgagtct gcttcttaac
      241 aggacagcca aagacaaacc acgcccgccc tgccggacgg gcgtggtttt ttgccgttta
      301 ctgtgtcttt ttcagctgct tagccagctg gtctgcgccg gtggcggcaa ggccgctgac
      361 aatgcccacc gccaccgcgg tgaccgggtc gccggccggg aaatccggca ccaccagcat
      421 gcacaccggc cccagcaccg cccccagcgc cccgcagatc gtgggcagcc acttgttcgc
      481 aagcggcgtc tgcttcatcc ccagcgccgc cagatagcag atcaccgtga tgcaggccac
      541 ccccgcaatg ccaaacccgt tcagttccat ttacttttcc tccttgatgt cttctttgat
      601 ctcgtcaatc tgcttccagg cgctcttcag gtcccgctcg gccaccgcca tccgctccac
      661 cacactgttg tgcttgtcca ccttttgctc caacatcttc agccgctggg tcacgctgcc
      721 ccaggtggcc gcaaaggtga ccgcgtacac cagaatctgc acccaaaagc tcgtgtcaaa
      781 ctgcggcatc agagcacctc cagcgcggcg gcggccgcct tgatcctttt gagatactgc
      841 gccgcccggc gggccgcttc ggcgctctgc gcctctgccg ccgaggcccg ggccagcagg
      901 ccggcgttgc tgctctccag ctctttgacc cgccgggcca gagccgccgc gtcgccgccc
      961 gcgccctggg cctgcaccgc ccgcaccgcg tccccggcgg aaaggccggt gatcctgcag
     1021 cggtcgtcca gcaccacggc gtagcgctcc gcgcccccgg ccagcacccg cacccagcgg
     1081 tagccgtcag cgtcgaggcc gcagtcggcc atgagggggt aatatgtacc gctcgccagc
     1141 gtgccgttgt tgtaggacct gtccaccgcg tccacgtccg gcccggtgaa gcactggcag
     1201 tttttgctgc cgaacacctc cagaaattgc atcatgtcgt tcttgtcctc cttttgtccg
     1261 ccctggcggc cgatcagggc ggggtaatcc ttgtaggcga tgttgagatc gacccggccc
     1321 gaaatgccgt ccacccggcc gtcgctggtg tgctgccaga tgctcgcgcc tttgagcccc
     1381 atatacccgc ggtagtcggc cagccagagg tcgtaggccg acagagcgct catgtccagc
     1441 cagctgttcg caaagctggt gtaggtgtag agcaggggat aatatccttg cacctttgcc
     1501 tcctccaaaa aggccgccgc gacggcgttg ctttgggccc ggcccaaggc tttgtaggtg
     1561 gccgcgtcct cgatgtcaaa ggccagcggc atggtcagcc ggtagggggc cagcagggcc
     1621 gcggcctccc gggccgccct gcgggcgg
//
LOCUS       DBMDYA010000659         1646 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14621_length_1646_cov_1.634304, whole genome shotgun sequence.
ACCESSION   DBMDYA010000659 DBMDYA010000000
VERSION     DBMDYA010000659.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14621_length_1646_cov_1.634304"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="ACZPFK_13055"
     CDS             complement(<1..569)
                     /locus_tag="ACZPFK_13055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0061711 - tRNA
                     N(6)-L-threonylcarbamoyladenine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1407281.1"
                     /translation="MLILGIESSCDETAASVVEDGRIVRSNVVASQAAEHNLYGGVVP
                     EIASRRHIEAISGVAASALAEAGVCAGDLEAVAVTLAPGLIGGVLVGVNFAKGLALAA
                     NKPLVPVHHLRGHIAALYLTHPQLEPPFLCLVASGGHSHIIQVLDYTRFRVLGRTVDD
                     AAGEAFDKVARTLGLGYPGGPAVARAAEGG"
     gene            complement(697..1146)
                     /locus_tag="ACZPFK_13060"
     CDS             complement(697..1146)
                     /locus_tag="ACZPFK_13060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1407282.1"
                     /translation="MAAAQGLRFAPVAPRDVPALAALHADAPDPWAAAQLRAELEKPE
                     CRAFAAFADGAPAGFACFSLLGDTAELQTVAVARALRGGGIGGALLRHAFAALRRQGA
                     ARCLLEVRCSNAAARALYRKLGFETLALRRGLFSSPREDGETMELAL"
     gene            complement(1146..>1646)
                     /locus_tag="ACZPFK_13065"
     CDS             complement(1146..>1646)
                     /locus_tag="ACZPFK_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1407283.1"
                     /translation="IVYGIGAFSLAKDVGVSVKEADTFIKNYLSEYSGVKSYMEKTVE
                     QGREKGYVATLYGRRRPLPELTAANRNIRALGERMAMNTPIQGTAADVIKLAMIRVFR
                     RLEAEGLKAKLVLQVHDELIVEAPEAEADQAAAILGEEMEHAADLAVRLTAEVNRGRT
                     WYEAKG"
BASE COUNT          237 a          657 c          505 g          247 t
ORIGIN      
        1 cccccctcgg cggcccttgc caccgccggc cctcccggat atcccaggcc cagtgtgcgc
       61 gccaccttgt caaaggcctc tcccgccgcg tcgtccaccg tgcggcccag cacccggaag
      121 cgggtgtaat cgagcacctg gatgatgtgg ctgtggccgc cgctggcgac caggcacaaa
      181 aaaggcggct ccagctgcgg atgggtcagg taaagcgccg caatatgccc gcgcagatgg
      241 tgcaccggca ccagcggctt gttcgcggcc agggccagcc ccttggcgaa gttgaccccc
      301 accagcacgc cgccgatcag ccccggggca agggtcaccg ccaccgcctc cagatccccg
      361 gcgcaaacgc ccgcctcggc cagcgccgac gcagccaccc cgctgatggc ctcgatgtgg
      421 cggcgggagg cgatctccgg caccacaccg ccgtaaagat tgtgttcggc ggcctggctg
      481 gccaccacgt tgctgcgcac aatgcggccg tcctccacca ccgaggcggc ggtctcgtcg
      541 cagctggatt cgatccccaa gatcagcatt ttcctctccc ccgcccgcgc cgccctctct
      601 gggcccgcgg cctcttttca agcgtcaagc gcggggcgac ggccccgccg ccgccccgct
      661 gctgtatcct cttcggtccg cgccgctctc cgccgctcag agggccagct ccatggtctc
      721 accgtcctcg cgcgggctgc tgaacagccc ccggcgcagc gccagcgtct caaagccaag
      781 cttgcgataa agcgcccttg cggccgcgtt ggagcagcgc acctccagca ggcagcgcgc
      841 cgcgccctgc cgccgcaggg cggcaaaggc gtggcgcagc agcgccccgc caatcccccc
      901 gccgcgcagg gcgcgggcca cggccaccgt ctgcagctcg gccgtatccc ccagcaggga
      961 aaagcaggca aagcccgccg gcgcgccgtc cgcaaacgcc gcaaaggccc ggcactccgg
     1021 cttttccagc tccgcccgca gctgcgccgc ggcccaggga tcgggcgcat cggcgtgcag
     1081 cgccgccagc gcgggcacgt cccgcggcgc gacaggcgcg aagcgcagcc cttgtgcggc
     1141 cgccatcagc cctttgcctc gtaccaggtg cggccccggt ttacctccgc ggtcagcctc
     1201 accgccaggt cggcggcgtg ctccatctcc tcgcccaaaa tcgccgcggc ctgatccgcc
     1261 tccgcctccg gcgcctccac gatcagctcg tcgtgcacct gcagcaccag ctttgctttc
     1321 agcccctcgg cctccagacg ccggaagacc cggatcatcg ccagcttgat cacgtcggcg
     1381 gcggtgccct ggatgggcgt gttcatggcc atccgctcgc ccagggcgcg gatgttgcgg
     1441 ttggccgcag tgagctccgg cagggggcgg cggcggccgt acagggtcgc cacatagccc
     1501 ttttcccggc cctgctccac cgttttttcc atatagcttt tcacgcccga gtactcggac
     1561 aggtagtttt tgataaaggt gtcggcctct ttcaccgaga cccccacgtc cttggccagg
     1621 ctgaacgccc cgatgccgta gacgat
//
LOCUS       DBMDYA010000660         1645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14641_length_1645_cov_1.067358, whole genome shotgun sequence.
ACCESSION   DBMDYA010000660 DBMDYA010000000
VERSION     DBMDYA010000660.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14641_length_1645_cov_1.067358"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="ACZPFK_13070"
     CDS             <1..278
                     /locus_tag="ACZPFK_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR1407284.1"
                     /translation="RCRFFVADVADFAPAQRSLVLTNPPYGERLGDLAEAGRLEKTLG
                     AQLQQSPVSGAYIITADAEFEQNFGKKAARRRKLYNGMIPCQLYMYF"
     gene            complement(602..>1645)
                     /locus_tag="ACZPFK_13075"
     CDS             complement(602..>1645)
                     /locus_tag="ACZPFK_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407285.1"
                     /translation="ATLFGWAILFSCLYLLCRLAFARDRRVAPLLGLMAGALPLLHTH
                     SFLALAVVSAVLLIYALLDRERPPRQLAAWLLYAGLAAAAALPQVLCFTLGASGAQHF
                     VRFSFNWVNAGRDDYFWFYIKNIGLVYLLAPLAFFRAPRPQRWLYGGGLALLALCEFI
                     VFQPNAYDNNKLLYIWHFMGCMLAAELLWQFAAGLRRRRWRRAAAGLAVFCATFGSLL
                     TVGRELVSGYQQFGAAEVRAAAWAESATSPAALFLTADNHNNALASLAGRRILCGSGS
                     YLYYHGLDYGDQYGAMQRLLTDPDEALLAQWGIDYAWFSSYERAADGAEAWYAERYPA
                     VYSADGVTVYRIR"
BASE COUNT          327 a          522 c          561 g          235 t
ORIGIN      
        1 agcgctgccg cttttttgtg gcggatgtgg ccgactttgc ccccgcccag cgcagcctgg
       61 tgctcaccaa ccccccgtac ggggagcggc tgggagacct ggccgaggcg ggccgcctgg
      121 aaaaaacgct gggcgcgcag ctgcagcaga gcccggtctc aggcgcgtat atcatcacgg
      181 cggacgccga gtttgagcag aatttcggca aaaaggccgc gcggaggcgc aagctttaca
      241 acggaatgat cccctgtcag ctctatatgt atttctagct ggagtgaaat agagttttgc
      301 gcatcgtttg agtcgccggg ctttcccgcg acggatgcaa aggccccggc gctttctcct
      361 caaaaaagaa agcgccgggg cctttgcctg ctttgagagg cggcaggatc cagcgacgag
      421 gttctcggat atccatagcc tcggtgccga tgcccagggg gcaggcggca gggacctggc
      481 gcgcggaccg gggggatctt ttgcccaagg cccgttcggc gggacacagc ccttttcacg
      541 gggttctctc atccgggcgg gtgcggcgga cgccggaaag ggattgtccc gcaaagcgcg
      601 ctcagcggat gcggtacacc gtcacgccgt ccgccgaata gaccgcggga taccgctccg
      661 cgtaccaggc ctccgcgccg tcggcggcgc gctcgtaaga ggagaaccag gcgtagtcga
      721 ttccccattg cgccaaaagc gcctcgtccg ggtcggtgag caggcgctgc atcgcgccgt
      781 actggtcgcc gtagtccagc ccatgataat aaagatagct gccgctgccg caaaggatgc
      841 gcctgcccgc caggctggca agagcgttgt tgtggttgtc tgcggtgaga aaaagtgcag
      901 ccgggctggt ggcgctctcg gcccaggcgg cggcgcgcac ctcggccgcg ccgaactgct
      961 ggtaaccgct caccagctcg cgccccaccg tcagcaggga gccgaaagtg gcgcaaaaga
     1021 ccgccagccc ggccgcggcc ctgcgccagc ggcggcggcg cagcccggcg gcaaactgcc
     1081 acagcagctc cgccgccagc atacagccca tgaaatgcca gatgtagagc agtttgttgt
     1141 tgtcataggc gttgggctga aagacgatga actcgcacag cgccagcagt gccagcccgc
     1201 ctccgtagag ccagcgctgg ggccgagggg cgcgaaaaaa ggccagcggg gccagcaggt
     1261 agacaaggcc gatgtttttt atgtaaaacc agaagtagtc gtcccgcccg gcgttgaccc
     1321 agttgaaaga gaaacggaca aaatgctgcg cgcccgaggc ccccagcgtg aagcagagca
     1381 cctgcggcag ggccgcagcc gcggcgagcc ccgcgtagag cagccaggcg gccagctggc
     1441 ggggagggcg ctcccggtcc agcagggcat agattaacag aaccgcgctc accaccgcca
     1501 gcgccaaaaa ggagtgggtg tgcagcagcg gcagggcgcc ggccatcagc cccagcagcg
     1561 gcgccacgcg ccggtcgcga gcaaaggcga ggcggcacag cagatacagg cagctgaaga
     1621 gaatagccca gccgaacagc gtggc
//
LOCUS       DBMDYA010000661         1644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14650_length_1644_cov_1.329229, whole genome shotgun sequence.
ACCESSION   DBMDYA010000661 DBMDYA010000000
VERSION     DBMDYA010000661.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14650_length_1644_cov_1.329229"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="ACZPFK_13080"
     CDS             complement(<1..517)
                     /locus_tag="ACZPFK_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1407286.1"
                     /translation="MLQRPELLAPAGDMERLRFALLYGADAVYLAGDRFGMRGAPKNF
                     SLDELEEAAKLCHAAGKRMYVTVNTMPTNEEIDALPGYLAALREIGPDALIVADLGVL
                     SLAKRYAPGLELHMSTQAGIVNWAAAAAAYELGAKRVVLARELTLEEIAGIRAKAPAG
                     LEIEAFVHGAMC"
     gene            complement(677..>1644)
                     /gene="mltG"
                     /locus_tag="ACZPFK_13085"
     CDS             complement(677..>1644)
                     /gene="mltG"
                     /locus_tag="ACZPFK_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1407287.1"
                     /translation="QFAAIADRLEEEGIIGSGFFFRLYLKQTGAGPTLQYGTFTLAPG
                     MSYDDIIAELQTPAKRDVVRLTFPEGTPALGIAQSMEDAGLCTAEEFLACANGEDGSD
                     FSQYEFWGQIPESAARFMKCEGYLFPETYEFHKDDSVYNYVDTFYGEFDRRVDAELRQ
                     KIADSGMTLDEAVILASFVQEEAGNDEDKNVAEVFLNRLADGSPYPRLESNASSYVQN
                     PDDNNYLYNWVAPYYGGWENIPAELYAAYNTYEHAGLPAGAISNPGLDAIRATVEPNT
                     ALVSENGNSPCYFFVTDLTGRYYYAATAEEHQANVNKAWKVNGSL"
BASE COUNT          256 a          563 c          508 g          317 t
ORIGIN      
        1 tacacatggc cccgtgcaca aacgcctcga tctccagccc ggccggcgct tttgcgcgga
       61 tgcccgcgat ctcctccagc gtcaactccc gcgccagcac cacccgcttc gcccccagct
      121 cgtaggcggc ggccgccgcg gcccagttga cgatgcccgc ctgggtggac atgtgcagct
      181 ccagcccggg ggcataccgt tttgccagcg agagcacccc caaatccgcc acgatcagcg
      241 cgtccgggcc gatctcccgc agggcggcca gatacccggg cagggcgtcg atctcctcgt
      301 tggtgggcat ggtgttcacg gtcacgtaca tccgcttccc cgccgcatgg cagagcttcg
      361 cggcctcctc cagctcgtcc agcgaaaaat ttttcggcgc gccgcgcatg ccgaaccgat
      421 cccccgccag atagaccgcg tccgcgccgt agagcagtgc aaagcgcagc cgttccatat
      481 cccccgccgg ggccagcaac tccgggcgct gcagcatagt ttcttcctcc tcaaaaagca
      541 ccgggccttt ttggaaaccg gcccggtgta tccatctttt attgtcccgc gcggtttgcc
      601 gggcgccgag agcgcgcaca gcacagcgcg ggcccgcttt tcggcccgtg gcagacgctt
      661 tgtcccctcc gccctgctac aggctgccgt tcactttcca ggctttgttc acgttggcct
      721 ggtgctcctc ggccgtggcg gcgtaatagt acctgccggt caggtcggtg acgaagaaat
      781 agcaggggct gttcccattt tcgctcacta aggcggtgtt gggctccacc gtggcccgga
      841 tggcgtccag gcccgggttc gagatcgcgc cggcgggcag gcccgcatgc tcataggtgt
      901 tgtaggcggc gtacagctcg gcggggatgt tctcccagcc gccgtaatag ggggccaccc
      961 agttgtacag gtagttgttg tcatccgggt tctgcacata gctgctggcg ttgctctcca
     1021 gccgcgggta gggcgagccg tcggccagcc ggttcaaaaa gacctccgcc acgtttttgt
     1081 cctcgtcgtt gcccgcctcc tcctgcacaa aggaggccag gataaccgcc tcgtccaggg
     1141 tcatgccgct gtccgcgatc ttctggcgca gctcggcgtc cacccggcgg tcaaattcgc
     1201 cgtaaaaggt gtccacataa ttgtagacgc tgtcgtcctt gtggaactcg taggtctcgg
     1261 ggaacaggta accctcgcac ttcatgaagc gggcggcgct ttccgggatc tggccccaga
     1321 attcatattg tgaaaagtcg ctgccgtcct cgccgttggc gcaggcgagg aactcctcgg
     1381 ccgtgcacag gcccgcgtcc tccatgctct gggcgatgcc cagggcaggc gtcccctccg
     1441 ggaaagtcag ccgcaccacg tcccgcttgg ccggggtctg cagctcggcg atgatgtcgt
     1501 cgtagctcat gccaggggcc agcgtgaaag tgccgtattg cagcgtgggc ccggcgccgg
     1561 tttgcttcag gtagaggcgg aagaaaaagc ccgagccgat gatgccctcc tcctccaggc
     1621 ggtcggcgat ggcggcgaac tgct
//
LOCUS       DBMDYA010000662         1637 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14724_length_1637_cov_1.461589, whole genome shotgun sequence.
ACCESSION   DBMDYA010000662 DBMDYA010000000
VERSION     DBMDYA010000662.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_1637_cov_1.461589"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="ACZPFK_13090"
     CDS             <1..901
                     /locus_tag="ACZPFK_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR1407288.1"
                     /translation="GGFAGFDGDIDLGDIFGSFFGGGFGGGRRQANPNAPRRGADIRV
                     SVALSFMEAAHGCTKTVTVNHQDTCPECGGSGAAKGTSPETCPDCGGRGTVTTQTRTP
                     FGVMQSQRPCSRCGGRGKIIKTPCRKCSGTGRVSSSKKLEVKIPAGIDDDQSLAMRGL
                     GDKGLNGGPAGDVIVIVTVRPDTLFERDKYDVWVTVPITYSQAVIGDEVVVPTIDGKV
                     QYKVPEGTQSGTVFRLRGKGIQYLNGKGRGDQYVKVNVEIPKKLTRTQRDALKKFEET
                     LKDENYEQRKGFFKTLKDMFGNS"
     gene            complement(1341..>1637)
                     /locus_tag="ACZPFK_13095"
     CDS             complement(1341..>1637)
                     /locus_tag="ACZPFK_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1407289.1"
                     /translation="PGLRRCWATGLAGLGFSLLIEGSQLLNIRVTDVDDLTANFAGAL
                     LGYAAWRLAARFCPWLRSQGRRAAGEPLLYIAAMFLGRFLLFDEFRLAKLLYGF"
BASE COUNT          290 a          539 c          547 g          261 t
ORIGIN      
        1 cggcggcttc gccggttttg acggcgacat cgacctgggc gatatcttcg gcagcttttt
       61 cggcggcggc ttcggcggcg ggcgccgtca ggccaacccc aacgcgccca ggcgcggcgc
      121 ggatatccgg gtgtcggtgg ccctctcttt catggaggcg gcccacggct gtaccaagac
      181 cgtcacggtc aaccaccagg acacctgccc cgagtgcggc ggcagcggcg cggccaaggg
      241 caccagcccc gagacctgcc cggactgcgg cggccggggc accgtgacca cccagacccg
      301 cacccccttt ggcgtgatgc agagccagcg gccctgctcg cgctgcggcg gccggggcaa
      361 gatcatcaag accccctgcc gcaagtgctc gggcacgggg cgggtctcca gcagcaaaaa
      421 gctggaggtc aagatcccgg cgggcatcga cgacgaccag agcctggcca tgcgcggcct
      481 gggcgacaag ggcctgaacg gcggccctgc gggggatgtg atcgtcatcg tcaccgtgcg
      541 cccggacacg ctgttcgagc gggataagta cgacgtctgg gtgacggtgc ccatcaccta
      601 ttcccaggcg gtgatcggcg acgaggtggt ggtgcccacc atcgacggca aggtgcagta
      661 taaggtgccg gagggtaccc agagcggcac ggtgttccgg ctgcggggca agggcatcca
      721 gtacctgaac ggcaagggcc gcggcgacca gtatgtcaag gtcaacgtgg agatccccaa
      781 aaagctgacc cgcacccagc gagacgcgct gaaaaagttt gaggagaccc tcaaggacga
      841 gaactatgaa cagcgcaagg gctttttcaa gaccctgaaa gatatgttcg gcaacagttg
      901 ataaaagtgg ggcccgcaca acgcgggccc cactttttgc agcttcttgc gggcggggaa
      961 aagaagcatc cgctgcatgc cgccgatggc aacggcggtg ttttttagcg tttgccgcgg
     1021 cgtcccccat ttttttgtcg gcgcattgca cccgcggccc tgtcggcagg gcctcgccgc
     1081 gtcacctctt cttgccgggg gcaggatcgg cgcgccctaa gccttgcttt tgaccgcggc
     1141 acagatggcc tttttcgtgt ggcccgcact gcggcggccg ctgtatggct gccccctttc
     1201 aagggagctg accgccgcag gccgtctgag agagcatgtc acggcgaagc gcctttttgc
     1261 attttccgcg gcgcgaccgc tttctttacc ccctttgcac ccgtggcttt tccagcgcgg
     1321 cctctctctc acggcaggcc tcaaaagccg tacagcagct ttgcgaggcg gaattcgtcg
     1381 aacagcagaa agcggccgag aaacatcgcc gcgatgtaga gcagcggctc gccggcggcg
     1441 cgccgcccct ggctgcgcag ccaggggcag aagcgggcgg ccagccgcca ggccgcgtag
     1501 cccagcagag cgccggcaaa atttgcggtc aggtcgtcca catcggtgac ccggatgttc
     1561 agcagctggc tgccctcgat cagcagcgag aagcccaacc cggctaggcc cgtggcccag
     1621 cagcgccgca ggccggg
//
LOCUS       DBMDYA010000663         1628 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14841_length_1628_cov_1.324820, whole genome shotgun sequence.
ACCESSION   DBMDYA010000663 DBMDYA010000000
VERSION     DBMDYA010000663.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14841_length_1628_cov_1.324820"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="ACZPFK_13100"
     CDS             <1..945
                     /locus_tag="ACZPFK_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407290.1"
                     /translation="GAPFTGLTDYSFELDDEGNPYWVISTYRNTWGFRLPEATGALII
                     NAETGARQKYTLSNLPEWVDRVQPEDFIIQQIDNKGQYINGFLNFADKDKYKTSPGDI
                     IVYNNGDCYLFTGITSVGADESAIGFVMVDMVTKEPTIYRISGATEQSAQKSAEGKVQ
                     QFGYYASWPIIVNVNGTPSYFMTLKDDEGLLKQYAYVSVKDYLVVGVGESVPEAKVNY
                     EKALRQNVSNDTTIAETTAQTASGTVQRISAEYNTSGTVYRFVLEGRPDKLFTAAASL
                     SEELAITREGDSVRVEYLDNGAGLCEVTGFDNLAFEQK"
     gene            1212..1625
                     /locus_tag="ACZPFK_13105"
     CDS             1212..1625
                     /locus_tag="ACZPFK_13105"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1407291.1"
                     /translation="MDKKALIEQAIAARENAYTPYSHFKVGAALLGRSGRVYTGCNVE
                     NAGYTPTNCAERTALFKAVSEGEREFEAIAIVGSMEGEKNELVTGPCGVCRQALYEFG
                     GDGLVVLMAKSPEDYVERTLGELLPFGFGPANLKK"
BASE COUNT          349 a          490 c          524 g          265 t
ORIGIN      
        1 ggcgcgccct ttaccggcct caccgactac agctttgagc tggacgacga gggcaacccc
       61 tattgggtga tctccaccta tcgcaacacc tggggcttcc gtctgccgga ggcgaccggc
      121 gcgctgatca tcaacgccga gaccggtgcg cggcagaaat atactctcag caacctgccc
      181 gagtgggtgg accgagtgca gcccgaggat ttcatcatcc agcagatcga caacaagggc
      241 cagtatatca atggcttttt gaactttgcc gacaaggaca agtacaagac ctcgccgggg
      301 gacatcatcg tctacaacaa tggagattgc tacctcttca ccggcatcac ctcggtgggc
      361 gcggacgagt cggccatcgg ctttgtgatg gtggacatgg tcaccaagga gcccaccatc
      421 taccgcatca gcggcgccac cgagcagagc gcgcagaagt cggccgaggg caaggtgcag
      481 caattcggtt attacgccag ctggcccatc atcgtcaacg tcaacggcac ccccagctac
      541 ttcatgaccc tgaaagacga cgagggcctg ctgaagcaat acgcctatgt gtcggtgaaa
      601 gactatctcg tggtcggggt gggcgagagc gtgcccgagg caaaagtcaa ctacgaaaaa
      661 gcgctgcggc agaacgtgtc caacgacacc accattgccg agaccacggc ccaaacggcc
      721 agcggcacgg tgcagcgcat ttcggcggag tacaatacct cgggcaccgt ctaccgcttt
      781 gtgctggagg ggcggccgga taagctcttc accgccgccg cctccctgtc ggaggagctg
      841 gccatcaccc gcgagggcga cagcgtaagg gtggaatatc tggacaacgg cgcgggcctg
      901 tgcgaggtca ccggctttga caacctggcc tttgagcaga agtagcggcc gtccggcgcg
      961 ccgggcagac ggcggcgttg gcctcttgcc tgcccggtga aaaacagcga aaaagagggg
     1021 cgagaaagac cggcagggcc gggctttctc gcttttctct atgtcatgtc cgtccgcacc
     1081 gtctcttgca ccgccgcgca ccgctggccc ctctggcgcg cggtgcggca aaatgatata
     1141 ctgaagaaaa tgacgccggg cgccgcaccg cggcggcgca gcggcgcgag acgccggaaa
     1201 ggagcagcaa catggacaaa aaagcactga tcgagcaggc cattgccgcc agggagaacg
     1261 cctatacccc ttattctcac tttaaggtgg gggccgccct gttgggcagg agcgggcgcg
     1321 tgtacaccgg ctgcaacgtg gaaaacgcgg ggtacacccc caccaactgc gcggagcgca
     1381 ccgctctgtt caaggcggtg agcgagggcg agcgggaatt tgaggccatc gccatcgtcg
     1441 gcagcatgga gggggagaag aacgagttgg tgaccgggcc ctgcggcgtg tgcaggcagg
     1501 cgctttacga gttcggcggc gacggcctcg tcgtgctgat ggccaaaagc ccggaggatt
     1561 atgtggagcg cacgctgggc gagctgctgc cctttgggtt tggcccggcc aatctgaaaa
     1621 aataaacg
//
LOCUS       DBMDYA010000664         1627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14856_length_1627_cov_1.095675, whole genome shotgun sequence.
ACCESSION   DBMDYA010000664 DBMDYA010000000
VERSION     DBMDYA010000664.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14856_length_1627_cov_1.095675"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            362..1270
                     /locus_tag="ACZPFK_13110"
     CDS             362..1270
                     /locus_tag="ACZPFK_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407292.1"
                     /translation="MSFSENLQFYRQQGGLTQEQLAETLGVSRQSVSKWESGQSYPEM
                     EKLLALCELFRCDLDTLLKGSAIVASADVGRAYDAYKNRSAAMSAVGVGILIVGLALA
                     CLLDGLGMHEMVAGLAFLGTALVGCMVLIVSGLLSSEFNRKHPRMEDCYSEAQRDAFH
                     SRYVVLTASSLGILFLSLLWLLGCQALPLPAFWRDGLSGSIFFMGVALGVSLLIWTGI
                     QKEKYNIEEYNLENDPDDETRARRGRIGSICACIMMAATAAFVVCIFALPQRLGEAAA
                     ILYSVGGIGCGVAAVALNRAGKGEKK"
BASE COUNT          269 a          525 c          541 g          292 t
ORIGIN      
        1 gcttccgcct tgccgcggtg ggacaaaagc ggcaaggcgg aagcgccgag gcggaactgg
       61 cgaaaaacgg cccgtccgga aaagttccgg caggccgcac agccgtaccg gggtaaaatc
      121 cgggccggga caaaaagtgc ccccgcaaaa gcgcgccgcc cggcagccgc cctgcggagc
      181 cgcggccggt gtgaaaaaag gcgatgcaac cgcaacttta catcgggaaa ccgaaacgca
      241 acagcaaatt ggtggaggtt gccgtccctc cacggtagac tgggagcata acgagggggc
      301 ccctctccgc cgcccagggc ggcaggcggg tcccctcatc aaccgcaagg aggagcttgt
      361 catgagtttc agtgaaaatc ttcagtttta ccgccagcag ggcggcctca cccaggagca
      421 gctggcggag acgctgggcg tctcgcgcca gtcggtctcc aagtgggagt cgggccagag
      481 ctatcccgag atggaaaagc tgctcgccct ctgcgagctg ttccgctgcg atctggacac
      541 gctgctgaaa ggcagcgcca tagtcgccag cgccgacgtg ggccgggcct acgacgccta
      601 taaaaaccgc tcggcggcga tgagcgccgt cggggtgggc atcctcatcg tcggcctcgc
      661 cctcgcctgc ctgctggacg gtctggggat gcacgagatg gtggccggcc tggccttttt
      721 ggggacggcg ctggtcggct gcatggtgct gatcgtcagc ggcctgctct ccagcgagtt
      781 caaccgcaag cacccgcgca tggaggactg ctacagcgag gcgcagcggg acgcctttca
      841 cagccgctat gtggtgctga cggcctcctc gctgggcatc ctttttctca gcctgctctg
      901 gctgctgggc tgccaggcgc tgccgctgcc cgcgttctgg cgcgacggcc tctccggctc
      961 gatcttcttt atgggcgtgg cgctgggcgt ctcgctgctc atctggaccg gcattcaaaa
     1021 agagaaatac aacattgagg agtataacct ggagaacgac ccggacgacg agacccgcgc
     1081 ccgccgcggc aggatcggca gtatctgcgc ctgcatcatg atggcggcca ccgccgcctt
     1141 tgtggtctgc atctttgccc tgccccagcg gctgggggag gcggctgcca tcctctactc
     1201 ggtgggcggc atcggctgcg gcgtcgccgc agtggccctc aaccgcgccg gcaaaggcga
     1261 aaaaaaatag cttcgccgcg cggcagtcaa aagccgggtc ggtgcggcgg gaggtgcaaa
     1321 gccgcgcccc tttccatggc cgcggccgca gggggagact tcccgcttcg ccgtgctgtt
     1381 gccgccgatc ccgggcaagg gaggtctcct ttggggctct ccgcggcgcg gttgttttcc
     1441 ttttttgcac gaggtgctcc tttgcgtccc atccgtcccg tgcgcccggc gtaaggcgtc
     1501 tgtttcagag gagcaaacga ccgcgcggct tttggaggga caagttgctg tttccatgtt
     1561 cgttcttgcc ggcgctgtcc ccggttcgcc cttccgcccc cacagagcgg gaatcctctt
     1621 tttgggg
//
LOCUS       DBMDYA010000665         1626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14861_length_1626_cov_1.711475, whole genome shotgun sequence.
ACCESSION   DBMDYA010000665 DBMDYA010000000
VERSION     DBMDYA010000665.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14861_length_1626_cov_1.711475"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="ACZPFK_13115"
     CDS             complement(<1..1239)
                     /locus_tag="ACZPFK_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1407293.1"
                     /translation="MEKMFKLKEHGTTVGKEVTGGFTTFFAMAYIIFVNPNFLSQTGM
                     DFNAVMLATCISAAIGTALTALLANVPFAQAPGMGLNAFFTFTVCFGMGYTWQQALAI
                     VFISGLLFLAITVSPLRKQVIDAIPAPLKNAISAGIGLFIALVGLLNAGIVVADGNLL
                     DLGHITSGAPLLALIGLLITGILMACGVKAAMLIGILVTTVIGIPMGLTTLPGEITLS
                     GISLAPTFMQMDFGGLLSMGVLPLITAIVTFSICDMFDTVGTLIGTAGNAGMLNEKGE
                     LPKGDRALIADAVATCVGACFGTSTVTTFVESSTGISEGARTGLSSVVVSLCFLASCV
                     LAPVAGIIPGAATAPALIIVGILMLKAAARIDWSDFEIAMPCFLTIAMMPFAYSITDG
                     IGFGFISWVVIKVFRGKFKEV"
     regulatory      complement(1416..1517)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          351 a          461 c          529 g          285 t
ORIGIN      
        1 cacttccttg aacttgccgc ggaagacctt gatcaccacc cagctgataa agccgaagcc
       61 gatgccgtcg gtgatggaat aggcaaacgg catcatggcg atggtcaaaa agcagggcat
      121 tgcgatctca aaatcgctcc agtcgatgcg cgcggccgcc ttgagcatca gaatgcccac
      181 gatgatcagg gccggggcgg tggcggcgcc ggggatgatg ccggccacgg gcgccagcac
      241 gcaggaggcg aggaagcaga ggctgaccac cacgctggag aggccggtgc gggcgccctc
      301 gctgatgccg gtggaggact cgacgaaagt cgtgacggtg gaggtgccga agcaggcgcc
      361 cacgcaggtg gcgacggcgt cggcgatcag ggcgcggtcg cccttgggca gctcgccctt
      421 ttcattcagc atgcccgcgt tgccggcggt gccgatcagg gtgcccacag tgtcgaacat
      481 gtcgcagata gaaaaagtga cgatggccgt gatcagcggc agcacgccca tgctcagcag
      541 gccgccgaaa tccatctgca taaaggtggg ggccagcgag atgccggaca gggtgatctc
      601 gccgggcagg gtggtcaggc ccatggggat gccgatgacg gtggtgacga ggatgccgat
      661 gagcatggcc gccttgacgc cgcaggccat gaggatgccg gtgatgagca ggccgatgag
      721 ggccagcagc ggcgcgccgg aggtgatgtg gcccaggtcg agcaggttgc cgtcggccac
      781 cacgatgccg gcgttgagca ggcccaccag ggcgatgaac aggccgatgc cggcgctgat
      841 ggcgtttttg agcggggcgg ggatggcgtc aatcacctgc ttgcgcagcg gggagacggt
      901 gatggccagg aagagcaggc cggagatgaa gacgatggcc agcgcctgct gccaggtata
      961 gcccatgccg aagcagacgg taaaggtgaa aaaggcgttg aggcccatgc cgggggcctg
     1021 ggcaaagggc acattggcca gcagcgccgt cagcgccgtg ccgatggccg ccgagataca
     1081 ggtggcgagc atcaccgcgt tgaaatccat gcccgtctgg gacaggaaat tcgggttgac
     1141 gaagatgatg tacgccatgg caaaaaaggt cgtaaagccg cctgtgacct ctttgccgac
     1201 ggtggtgcca tgctccttca gcttgaacat tttctccatg aaacaaaccc ctccattttt
     1261 ttggtgcgcg ttgccccgcg tccctatttc caaagggggc gccgccccca tatggcccct
     1321 gtgcgcaaac aaaaaagcgg ccggtgaccc ggccgccctg agcttctttt ctggaaaaaa
     1381 caaaatacaa aggaaagggc cgtcgctcgc gcaggccccg ttccgttctt cccaatagtc
     1441 gcgccattta cggcggcgcg gtagaaactc tggatccgta tcatccatat tatattggct
     1501 tattcatttt ttgccgcaac atcccgcaaa caaatttcac tttttgttca caatctaatt
     1561 ttaggccatc ccgttgggaa acgcaagccg cagttttgac caaactttct ctgaaatcga
     1621 cacaaa
//
LOCUS       DBMDYA010000666         1624 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14890_length_1624_cov_1.558109, whole genome shotgun sequence.
ACCESSION   DBMDYA010000666 DBMDYA010000000
VERSION     DBMDYA010000666.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14890_length_1624_cov_1.558109"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            65..979
                     /locus_tag="ACZPFK_13120"
     CDS             65..979
                     /locus_tag="ACZPFK_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407294.1"
                     /translation="MSDTAVKAARQPQGKQTKPPKKLARTGSALYIWQKICQNPLALA
                     GVIAICALVALSLISPYIMKYDYAAIDMANACATPSAEHLFGCDELGRDILSRILYGA
                     RYTLSVGVLSVAISATLGVVLGAVAGYFGGAADQIVMRFLDIMAAFPQLLLAIAISAV
                     LGTGFDKCIYALGISGIPHFARMMRANILTIRGQEFVEAATSINCSKARIIMKHVVPN
                     AIAPLIVEISMSIAGAGLAASSLSFIGLGVQPPAPEWGAMLASARNYIRLSPHMIMFP
                     GLFIMISVLSFNLIGDAIRDALDPKLKD"
     gene            1009..1506
                     /locus_tag="ACZPFK_13125"
                     /pseudo
     CDS             1009..1506
                     /locus_tag="ACZPFK_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1483..1624
                     /locus_tag="ACZPFK_13130"
                     /pseudo
     CDS             1483..1624
                     /locus_tag="ACZPFK_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          321 a          535 c          509 g          259 t
ORIGIN      
        1 atcaaggccc agtacgaggg ccagagcaaa aggaaagcaa aaaaagtgaa aggggtgagc
       61 gcaggtgtct gacaccgcag taaaagcggc gcggcagccg cagggcaagc agacaaaacc
      121 gccgaaaaag ctggcgcgca ccggctcggc gctttacatc tggcaaaaga tctgccagaa
      181 ccccctggcg ctggccggcg tcatcgccat ctgcgcgctg gtggcgctct cgctgatctc
      241 gccttacatc atgaaatacg actatgcggc catcgatatg gcgaacgcct gcgccacccc
      301 cagcgccgag cacctgttcg gctgcgacga gctgggccgc gacatcctct cccgcatcct
      361 ctacggcgcc cgctataccc tgagcgtggg cgtgctctcg gtggccatct cggccaccct
      421 cggcgtggtg ctgggcgccg tcgccggcta tttcggcggc gcggcggacc agatcgtgat
      481 gcgctttctg gacatcatgg cggcctttcc gcagctgctg ctcgccatcg ccatctcggc
      541 ggtgctgggc accggctttg acaagtgcat ctacgcgctg ggcatctcgg gcatccccca
      601 cttcgcccgc atgatgcgcg ccaacatcct caccatccgc ggacaggagt ttgtcgaggc
      661 ggcgacctcc atcaactgct ccaaggcccg catcatcatg aagcacgtgg tgcccaacgc
      721 catcgcgccg ctgatcgtcg agatctcgat gtccatcgct ggcgcagggc tggcggcttc
      781 ctccctcagc ttcatcggcc tgggcgtgca gccccccgcg cccgaatggg gcgccatgct
      841 ggcctcggcc cgcaactata tccgtctgtc cccccacatg atcatgttcc ccggcctctt
      901 catcatgatc agcgtgctga gcttcaacct gatcggcgac gccatccgtg acgcgctgga
      961 cccgaagctg aaagactgag aaaggcagga aagaaaatgg aaaacaagat ggcaaaagaa
     1021 gaccagcgtt tcctgtccgt gcgcgacctg gtggtggagt accgctcaga gggcaagacg
     1081 gtgcacgcgg tgaacggcgt ctcgttcgac ctcgccaagg gcaagaccct gggcctggtg
     1141 ggcgagacgg gcgcgggcaa gacctcgatc gccaaggcga ttctgcgcat tctgcccgac
     1201 cactccaccc agcgggccga gggcgtggtg gagctggacg gcgaggacat gatggccctg
     1261 cccgagcacg agatgcaaaa gctgcgcggg cacaaggtgt cgatgatctt tcaggacccg
     1321 atgaccgccc tcaacccggt caaaacggtg gccgaccaga tcgccgaggt ggtcaagctg
     1381 cacaacccgg tgaccaaggc cgaatcgctg gagcgggcca aaaagatgct ggagatggtg
     1441 ggcatctccg ccgaccgcgc gggcgagtac ccgcaccagt tctccggcgg catgaagcag
     1501 cgggtggtca tcgccatggc gctggcctgc gacccggagc tgctgatcgc cgacgagccc
     1561 acgacggcgc tggacgtgac catccaggcc caggtgctgg acatgatcaa caagctcaag
     1621 gagg
//
LOCUS       DBMDYA010000667         1621 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14913_length_1621_cov_1.453947, whole genome shotgun sequence.
ACCESSION   DBMDYA010000667 DBMDYA010000000
VERSION     DBMDYA010000667.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14913_length_1621_cov_1.453947"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="ACZPFK_13135"
     CDS             complement(<1..511)
                     /locus_tag="ACZPFK_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407295.1"
                     /translation="MTLKNVTKRYPGVVALSDVSIEVRRGEVLALIGENGAGKSTMIK
                     TITGAIQPDEGVITFEGQGYKALNPTLTRDLGIAAIYQEFTLAPTLTVAENIFMGQKV
                     NEGPVRNTKLLFEKAQQVLDRFGVDINPRSLVRDLTVAYKQLVEIAKAIAKNAKLIIM
                     DEPTAPLTDD"
     gene            complement(637..>1621)
                     /locus_tag="ACZPFK_13140"
     CDS             complement(637..>1621)
                     /locus_tag="ACZPFK_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1407296.1"
                     /translation="LALAMSLSLVACGGSDSSSSPAGSTAGAGSSAAGASTAGKDLSD
                     ITVAYVCSELTNEIFAMQVEAMEAYAKELGINFVYKQTKDTGEKITACENYISSGVDA
                     IICHVQDGPAMEDVVKEAHNAGVKFFAYDTDVEGADTFYGWDNYDYGYAIGTNAANWV
                     NATFEKGDTVYAASCNHTANFLIIREQGYKAALEELCPDITVEWVAEGIGGNAANGVT
                     AGENFLQVGKDINLVVGINDGGCLGVLEAFNAAGYGGDKVGIFAGDATNDALKAVKEG
                     GIYRGTVTTGLVSLAPDFIDLAVSLAQGEAVDRENFGPTTLITAENVDEFM"
BASE COUNT          260 a          485 c          530 g          346 t
ORIGIN      
        1 cgtcgtcggt cagcggggcg gtgggctcat ccatgatgat gagcttggcg tttttggcga
       61 ttgccttggc gatctccacc agctgcttgt aggcgacggt caaatcgcgc accagcgacc
      121 gggggttgat gtccacgcca aagcggtcga gcacctgctg cgccttttca aacagcagct
      181 tggtgttgcg caccgggccc tcgttgacct tctggcccat gaagatgttc tccgccaccg
      241 tcagggtggg ggccagcgtg aactcctgat agatcgccgc gatgcccagg tcgcgggtga
      301 gggtggggtt gagggccttg tagccctgcc cctcaaaggt gatgacgccc tcatcgggct
      361 ggatggcgcc ggtgatcgtt ttgatcatcg tggatttgcc cgcgccgttc tcaccgatca
      421 gcgccagcac ctcgccgcgc cgaacctcga tggagacgtc gctcagggcc accacgcccg
      481 ggtagcgctt ggtcacgttt ttcaaagtca aaatattgtc tgccatggta ttgtcagctc
      541 accgccttgt cacttgataa aaccgggggg atgtaagcat ttcctacatc cccccagatc
      601 ttgtttttac atacgcgtcc ggatcggagc gaagctttac atgaactcgt cgacgttttc
      661 ggcggtgatc agggtcgtcg ggccgaagtt ctcgcggtcc acggcctcgc cctgtgccag
      721 cgagacggcc aggtcgatga aatcaggggc cagcgagacc aggccggtgg tgacggtgcc
      781 gcgatagata ccgccctctt tgacggcttt cagggcgtcg ttggtggcgt cgccggcgaa
      841 gatgcccacc ttgtcgccgc cgtagccggc cgcgttgaac gcctcgagca cgcccaggca
      901 gccgccgtcg ttgatgccga ccaccaggtt gatgtccttg cccacctgca ggaagttctc
      961 gcccgcggtg acgccgttgg ccgcgttgcc gccgatgccc tcggcgaccc actccacggt
     1021 gatgtcgggg cacagctcct caagagcggc cttgtagccc tgctcgcgga tgatcaggaa
     1081 gttggcggtg tggttgcagg aggcggcgta aacggtgtcg cctttttcaa aggtcgcgtt
     1141 gacccagttg gcggcgttgg tgccgatggc atagccgtag tcatagttgt cccagccgta
     1201 gaaagtgtcg gcgccctcga cgtcggtgtc gtaggcaaag aacttgacgc ccgcgttgtg
     1261 cgcttctttg acgacgtctt ccattgcggg gccgtcctgc acgtggcaga tgatggcgtc
     1321 gacgccggag gagatgtagt tttcgcaggc ggtgatcttc tcgccggtgt ccttggtctg
     1381 cttataaaca aagttgatgc ccagctcttt ggcataggcc tccatggcct cgacctgcat
     1441 ggcgaagatc tcgttggtca gctcggagca gacataagcg accgtgatgt cggacaggtc
     1501 tttgccggcg gtggaggcgc ccgccgcgct gctgcccgcg ccggccgtgc tgcccgccgg
     1561 ggacgagctg ctgtcggagc cgccgcaggc gacgagggag agggacattg cgagtgccaa
     1621 t
//
LOCUS       DBMDYA010000668         1621 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14914_length_1621_cov_1.296711, whole genome shotgun sequence.
ACCESSION   DBMDYA010000668 DBMDYA010000000
VERSION     DBMDYA010000668.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14914_length_1621_cov_1.296711"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="ACZPFK_13145"
     CDS             complement(<1..908)
                     /locus_tag="ACZPFK_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR1407297.1"
                     /translation="MAKKKREGTITRRWVRGSLLVTILVLLIAEGVFVFYLNSYYYSG
                     ARTAIMTRVNTLTGQLSALSSASDAERSSTLRRMAEEFTEKDKFEFMLINTLGRVVST
                     SSGFVPDYLESLDDFEKAKKSADGIGEFVGTDSTGEHIMAVTYLVPKPAGGIMAVRFV
                     TGLSRVDNQIALLMVVSAAVVLVILFFSLFSGTYFIRSIVLPVNKVEQAATKIAAGDF
                     GARIDNVYNDELGRLCDTINHMAEELSKTDQLKNEFISSVSHELRTPLTSIKGWTETL
                     ARVDDPGDESYQKGLRIIMSETDRLYL"
     gene            complement(1377..>1621)
                     /locus_tag="ACZPFK_13150"
     CDS             complement(1377..>1621)
                     /locus_tag="ACZPFK_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1407298.1"
                     /translation="NGQPIDLTQVEFQIMELFFQNPGVALVRDKILEGVWGKNYYGDV
                     KIVDVNIRRLRMKIEDEPSSPTHIATVWGYGYRWKP"
BASE COUNT          320 a          515 c          453 g          333 t
ORIGIN      
        1 agatagaggc ggtcggtctc gctcatgatg atgcgcaggc ccttttgata gctctcgtcc
       61 cccgggtcat ccacccgggc cagcgtctcc gtccacccct tgatcgaggt gagcggggtg
      121 cgcagctcgt gcgagaccga agagatgaat tcatttttga gctggtcggt cttggacagc
      181 tcctccgcca tatgattgat ggtgtcgcac aggcggccca gctcgtcgtt gtagacgttg
      241 tcgatcctgg cgccgaaatc ccccgccgca atcttggtgg ccgcctgctc caccttgttg
      301 accggcagca cgatggagcg gatgaagtag gtgccggaaa agagggaaaa gaataagatc
      361 accagcacca ccgcggcgga gaccaccatc agcagggcga tctgattgtc cacccgggag
      421 aggccggtga caaagcgcac cgccataatg ccgcctgcgg gtttcggcac cagataggtg
      481 accgccatga tatgctcgcc ggtgctgtcg gtgcccacaa actccccaat gccgtccgcg
      541 ctcttttttg ctttttcaaa atcgtccagg ctctcgagat agtcgggcac aaagccgctg
      601 gaggtggata ccacccgccc cagcgtgttg atgagcataa actcgaattt atctttttcg
      661 gtgaactctt ccgccatccg ccgcagcgtg gagctgcgct cggcatcgct ggccgaggag
      721 agcgcggaca gctggccggt aagggtgttg acccgggtca tgatcgcggt gcgcgcgccg
      781 gaatagtaat agctgttgag gtaaaagaca aagacgccct cggcgatgag cagcaccagg
      841 atggtgacca gcagactgcc ccggacccag cgccgggtga tggtgccctc gcgtttcttt
      901 ttcgccacgc cgcttcatcc ctttcccttt cagtcccccc gccgcgtttc acaacacgcc
      961 gcagcagcgg ccgcccggct gcgcggccga tcctcaaatc ccggcacctt gtcccatccc
     1021 aacggcgccg ccgtccgctc aaaaagtgaa accttttttc gtaccaaagg cggggcagct
     1081 cactttttat ccccggatac cgtttccgtt tttcccgctc cgaaagacgg ccccgccgtc
     1141 ctcacatgga catgcagtac aattacagcg agggagcaga tcacatcatg tcctaaggtt
     1201 tcgcccccgc ttcggaggac ggccccgccg tcctcacatg gacatgcagt ataactacag
     1261 cgagggagca gatcacatca tgtcctaagg tttcgccccc gcttcggagg acggccccgc
     1321 cgtcctcaca tggacatgca gtacaaccaa agcgagggag taagtcacat catgtcctaa
     1381 ggtttccacc ggtatccata gccccagacg gtggcgatgt gggtcgggct gctgggctcg
     1441 tcctcgatct tcatgcgcag gcggcggata tttacatcca cgatcttcac gtcgccgtag
     1501 taatttttgc cccagacgcc ctccagtatc ttgtcccgca ccagagccac accgggattc
     1561 tggaaaaaga gctccatgat ctggaactcc acctgcgtca ggtcgatggg ctggccgttt
     1621 t
//
LOCUS       DBMDYA010000669         1615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_14968_length_1615_cov_1.279392, whole genome shotgun sequence.
ACCESSION   DBMDYA010000669 DBMDYA010000000
VERSION     DBMDYA010000669.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14968_length_1615_cov_1.279392"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            211..462
                     /locus_tag="ACZPFK_13155"
     CDS             211..462
                     /locus_tag="ACZPFK_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase to dihydroxyacetone kinase"
                     /protein_id="HIR1407299.1"
                     /translation="MLEFKFDTQLLIEGQNLSEDEIDAYISQNFAGDCLLAVGDEELI
                     KIHFHTNEPWKVLEYCASLGEIHDIVIENMERQADGLSG"
     gene            504..866
                     /locus_tag="ACZPFK_13160"
     CDS             504..866
                     /locus_tag="ACZPFK_13160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1407300.1"
                     /translation="MGEPNERLQKQMAFILEADKEKFIQRQTYLTGGERKENDAEHAW
                     HLALMTLLLSEYANREIDVLRTISMVLIHDIVEIDAGDTYAYDPVGKQTQRQRETKAA
                     DRLFGLLPEDQCRKLRDL"
     gene            873..1058
                     /locus_tag="ACZPFK_13165"
     CDS             873..1058
                     /locus_tag="ACZPFK_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407301.1"
                     /translation="MLNAAAGGKSWVEHATRLSSILSRHEGTKRGSETLWEFCRAQYL
                     EPNLANGKITDDCNGLN"
     gene            complement(1444..1614)
                     /locus_tag="ACZPFK_13170"
     CDS             complement(1444..1614)
                     /locus_tag="ACZPFK_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407302.1"
                     /translation="MFREPLTDLAGSVGISYRHMMRLMTALCREGLLGREPGGYRLLD
                     PRALRARASEFE"
BASE COUNT          337 a          527 c          508 g          243 t
ORIGIN      
        1 ttcggcatgt accgcggctg ggactacgcg ctgctcttct ccctcgcggc cctggtgctg
       61 gcgaacgccc tcttctggcc cctgcgcttt tggcgccgcc gcaaaaaaaa gcggctcgcc
      121 gccgcggacg caccgggcga ggcctagggc cataatcgcc gccggggcgg caatactgta
      181 ttaaaagcac acccaaaaag gaggaccatc atgctggagt tcaagttcga cacccaactg
      241 ctcatcgagg ggcaaaacct gtcggaggac gagatcgacg cctacatctc gcagaacttt
      301 gcgggcgact gcctgctggc ggtgggcgac gaggagctga tcaagatcca ctttcacacc
      361 aacgagccct ggaaggtgct ggagtactgc gcctcgctgg gcgagatcca cgatatcgtg
      421 atcgagaata tggagcggca ggcggacggc ctgtcgggct gaacggcgcg cggaattttt
      481 gaaatgcaaa ggagacggga gcaatgggcg agcccaacga gcggctgcaa aaacagatgg
      541 ccttcatcct ggaggccgac aaggaaaaat ttatccagcg gcagacctat ctgaccggcg
      601 gagagcgcaa ggagaacgac gccgagcacg cctggcacct ggcgctgatg accctgctgc
      661 tcagcgagta cgcaaaccgg gagatcgacg tgctgcgcac gatatcaatg gtgctgatcc
      721 acgacatcgt ggagatcgac gccggcgaca cctacgccta cgacccggtg ggcaagcaga
      781 cccagcgcca gcgggagacc aaggccgccg accggctgtt cggcctgctg ccggaggacc
      841 agtgccgcaa gctgcgggac ctgtagccta ccatgctcaa cgccgccgcg ggcggcaaat
      901 cctgggtgga gcacgccacc cgcctctcca gcatcctcag ccgccatgag ggcaccaagc
      961 gcggctccga gaccctgtgg gagttctgcc gcgcccagta tctggagccg aacctggcaa
     1021 acggcaagat caccgacgac tgcaacggcc tgaactaggt tgaaacaaag ggcgcgtgga
     1081 ggcaaaagtt cgccctttcg ggcggcaggc ccaggcgtcc ggtccaaaaa aggttggtcc
     1141 ccccgcgggg ggaccaacct tttttacaat gccttttctc ttgaaagcag cgcgcaaagg
     1201 ccgcggcgcg cacccggcac aaaaagcggc ggcgggggcc aaaaaacgcg tgcaccggtt
     1261 gcgcgctgtt tatctccgcc ctcttttcgc agaagccgca caagacgggg ctgccgcgcc
     1321 ctctggccac cccagggaaa ccggcggcgc ggcgggtaaa aatgcctctg ccaaagcaaa
     1381 ttttttcacc cctgcctgcc gaagcggaaa ggcggccgcc cccctctttt ttctctcttc
     1441 gcctcattca aactccgacg cacgcgcccg cagcgcccgg gggtccagca gccggtagcc
     1501 gcccggctcc cgccccagca ggccctcgcg gcagagggcg gtcatcaggc gcatcatgtg
     1561 ccggtagctg atgccgacgg aaccggcgag gtcggtgagc ggctcgcgga acatg
//
LOCUS       DBMDYA010000670         1611 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15016_length_1611_cov_1.633775, whole genome shotgun sequence.
ACCESSION   DBMDYA010000670 DBMDYA010000000
VERSION     DBMDYA010000670.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15016_length_1611_cov_1.633775"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="ACZPFK_13175"
     CDS             complement(<1..1448)
                     /locus_tag="ACZPFK_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1407303.1"
                     /translation="MFGEMADGLSLALLNAGFAAGAAVLVVLLARLALRRAPKLYSYL
                     LWAPVLFRLLCPFSIESVFSLLPAKAQPIPKDILYAGTPRVDTGFAALNRAVNSVLPA
                     GEPAMSVNPLQVWLAAAQLVWLAGAAGLLLYSLAAALRLRRRLKGAAREEENIYTAAG
                     LETPFVMGLFRPRIYLPAGLAGEERRYILLHEQTHIRRGDHLWRLLGFFALCLHWFNP
                     LVWLAFYLSGKDMEMSCDEAVLRALGENVKKPYSASLLALASGRRWVGGTPLAFGEGD
                     TGARIKNVLRYRRPAFWVSAALLAVVAAAAVGLALSPRSGREIGGAPSAAPSVPASSS
                     EVQAVPVPLEEQEKVFLAALFASASADGGVARFTVPPEIPAGHRLDIQVSAQIPRVEN
                     GVPYTEPVEGFQAQAEGTQAWQAGRAYSETLIEGDIPDGSHIGFSAWFVDEDGISETY
                     GHVGFSFKDGKAVREIDPHATGVRGTEAVSGGG"
     gene            complement(1451..1576)
                     /locus_tag="ACZPFK_13180"
     CDS             complement(1451..1576)
                     /locus_tag="ACZPFK_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407304.1"
                     /translation="MPRFFAAFTRRNRLSEAEVEQLRRMIEAYGETGGEEMRGEG"
BASE COUNT          296 a          603 c          486 g          226 t
ORIGIN      
        1 ccgccgccgc tcacagcttc cgtcccccgc accccggtgg catggggatc gatctccctt
       61 acggctttcc cgtctttgaa agaaaatccc acatgcccat aggtctccga aatgccgtcc
      121 tcatccacga accaggcgga aaagccgata tggctgccgt ccggaatgtc gccctcgatc
      181 agcgtctcgc tgtaggcgcg gcccgcctgc caggcctgcg tcccctccgc ctgcgcctga
      241 aagccctcta ccggctcggt atagggcacg ccgttttcca cgcgcgggat ctgcgcggac
      301 acctggatat ccagccgatg ccctgcgggg atctctgggg gcactgtgaa ccgggccacc
      361 ccgccgtcgg cggaggcgct ggcaaacagt gctgccagaa aaaccttttc ctgctcttcc
      421 agcggaaccg ggacagcctg cacctctgag gaggaggccg ggacggacgg cgccgccgac
      481 ggcgcgcccc cgatctcccg cccggaccgc gggctcagcg ccagccccac ggccgccgcg
      541 gccacgacgg ccaacagcgc cgccgagacc cagaaagcgg ggcgccggta gcgcagcaca
      601 tttttgatgc gcgcgccggt atccccctcg ccaaaggcca gcggcgtgcc gcccacccag
      661 cgcctgccgg acgccagggc cagcagcgag gccgaataag gctttttcac gttttcgccc
      721 agcgcccgca gcaccgcttc gtcgcaggac atctccatat ccttgccgct cagataaaac
      781 gccagccaga ccagcgggtt gaaccagtgc agacagagtg caaaaaagcc cagcagacgc
      841 cagaggtggt cgccgcgccg gatgtgggtc tgctcatgca gcaggatata ccgccgctcc
      901 tcgcccgcaa ggcccgcggg cagatagatg cggggccgaa agagccccat aacgaacggg
      961 gtctcaaggc ccgccgcagt gtagatgttc tcttcctccc gcgcagcccc tttcagcctg
     1021 cggcgcagcc gcagggctgc cgccaggctg taaagcagca ggcccgccgc gcccgcgagc
     1081 cagacgagct gcgcggccgc cagccagacc tgcagcgggt tcacgctcat ggcgggctcc
     1141 cctgcgggca gaacggagtt caccgcccgg ttcagcgccg cgaagccggt atccacccgc
     1201 ggcgtcccgg cgtaaaggat atccttcggg ataggctgcg cctttgccgg cagcaggctg
     1261 aacacgctct cgatggaaaa ggggcagagc agccgaaaga gcaccggcgc ccagagcaga
     1321 taagagtaca gctttggcgc ccgccgcagc gccagccgcg ccagcagcac caccagcacc
     1381 gctgcgccgg cggcaaagcc ggcgttgagc agtgccaaag acagtccgtc cgccatctct
     1441 ccaaacatgg tcaaccctcc ccccgcatct cttccccacc cgtttcgcca taggcctcga
     1501 tcatccgccg cagctgctcc acctcggcct cgctgagccg gttgcggcgg gtgaacgccg
     1561 caaaaaagcg gggcagcgag ccgccgaagc tctcttcaag gaattgctcc c
//
LOCUS       DBMDYA010000671         1608 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15047_length_1608_cov_1.468480, whole genome shotgun sequence.
ACCESSION   DBMDYA010000671 DBMDYA010000000
VERSION     DBMDYA010000671.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15047_length_1608_cov_1.468480"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="ACZPFK_13185"
     CDS             complement(<1..597)
                     /locus_tag="ACZPFK_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1407305.1"
                     /translation="MSGHSKWNNIKRKKEKTDGAKAAIFTKMAREIAVAVKEGGGPDP
                     ANNGKLRDVVAKAKANNVPNDNIARAIKKASDEDKANYESIMYEGYGPSGVAVMVETL
                     TDNRNRTASNVRHAFDKYGASLGTSGCVSFLFTKKGVLLIEAEGRDEDTVMEDCFDVG
                     AEDFEVGEDFMQITTDPNSLHDVREALEAKGYNFISAEV"
     gene            complement(756..>1608)
                     /locus_tag="ACZPFK_13190"
     CDS             complement(756..>1608)
                     /locus_tag="ACZPFK_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR1407306.1"
                     /translation="LVDAPCSGEGMFRKEPAALQQHSQRLIESCASLQRALLDAIAPA
                     LRPGGLLVYSTCTFSPEEDEGTVGAFLRDHPEFVLEDVEASFGCAGHTSCCLEGPIET
                     EKVRRIYPVHGGEGHFMAKLRRAGEGVSLAPGKGRLTPEKLPREAERFFDDCFPSLCD
                     APAALRGGKLLLLPQEPVFLPRGLRVLRAGVLAGELVQGRGRESRFEPHHSLYMAFGP
                     SCAHRLELSSGDARTGAWLRGEQIEAPGLAGGYTAVTVDGFPLGFGKASGGSLKNHYP
                     KGLRNLK"
BASE COUNT          271 a          563 c          473 g          301 t
ORIGIN      
        1 cacctccgcc gagataaagt tgtatccctt ggcctccagc gcctcgcgca cgtcgtgcag
       61 gctgttgggg tcggtggtga tctgcatgaa atcctcgccc acctcaaaat cctccgcgcc
      121 cacgtcgaag cagtcctcca tgaccgtgtc ctcgtcgcgg ccctcggcct caatcaatag
      181 cacgcctttt ttggtgaaca aaaacgagac gcagccgctg gtgcccaagc tggcgccgta
      241 tttgtcaaag gcgtggcgca cgtttgaggc cgtgcggttg cggttgtcgg tcagggtctc
      301 caccatcacc gccacgccgg aggggccgta gccctcatac atgatgctct cgtaattggc
      361 cttgtcctcg tcgctggcct ttttgatggc gcgggcgatg ttgtcgttgg gcacgttgtt
      421 ggccttggcc ttggccacca cgtcgcgcag cttgccgtta ttggccgggt ccgggccgcc
      481 gccctccttg accgccaccg cgatctcgcg ggccatcttg gtaaagatgg ccgctttggc
      541 gccgtccgtc ttctcttttt tgcgcttgat gttgttccat ttcgaatgac ctgacataat
      601 cttcctccgt atgaactccg gccgcagtcg cggcggacag atttaaaccg ccgccccggg
      661 cggcgtatga gacagaaaag ccagccgcgc ggcgccgttt tcagacgcgc cggctgaaag
      721 catcttaact ttatcagttt atcacaaacc gcagattatt tcaaatttcg cagccccttg
      781 ggataatggt ttttcaggct gccgccgctg gccttgccga accccaacgg aaagccgtcc
      841 accgtgaccg ccgtataacc gccggccagg ccgggggcct cgatctgctc cccccgcagc
      901 caggcgccgg tgcgggcgtc gccggatgat agctccagcc ggtgggcgca ggatggtcca
      961 aacgccatat aaaggctgtg atgcggctcg aagcggctct cgcggccgcg cccctgcacc
     1021 agctcgcccg ccagtacccc cgcccgcagt acccgcaggc cccggggcag aaacaccggc
     1081 tcctgcggca gcaacagcag ctttccgccg cgcagcgcgg cgggcgcatc gcaaagggag
     1141 gggaaacagt cgtcgaaaaa gcgctctgcc tcccggggca gtttttccgg cgtgaggcgc
     1201 cctttgcccg gcgcgagcga aacgccctcc cccgcgcggc gcagctttgc cataaaatgc
     1261 ccctcgcccc cgtggaccgg atagatgcgg cgcacctttt ccgtctcgat cggcccctca
     1321 aggcaacagg acgtatgccc tgcacatcca aacgacgcct ccacgtcctc cagcacaaac
     1381 tccgggtgat cgcgcaaaaa cgcccccacc gtgccctcgt cctcctcggg ggaaaaggta
     1441 caggtggaat agaccagcag ccccccaggc cgcagggccg gggcaatcgc gtccagcagc
     1501 gcgcgctgca gggaggcgca gctctcgatg aggcgttggc tgtgctgctg cagcgccgcc
     1561 ggctccttgc ggaacatccc ctccccgctg cagggcgcgt ccaccagg
//
LOCUS       DBMDYA010000672         1606 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15072_length_1606_cov_1.506312, whole genome shotgun sequence.
ACCESSION   DBMDYA010000672 DBMDYA010000000
VERSION     DBMDYA010000672.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15072_length_1606_cov_1.506312"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="ACZPFK_13195"
     CDS             complement(<1..429)
                     /locus_tag="ACZPFK_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1407307.1"
                     /translation="MAFTSLSFILFFPVAALGYYVVPQKLKNSWLLVMSYYFYVCFDI
                     VYSLFLLFSTLTTYFCGRLLERINASRARKMILIVTILLNLGLLFLFKYYNMFSALAV
                     RVFALLGITLVPAQLSLLLPVGISFYTFQVLGYSIDVYRET"
     gene            696..1580
                     /locus_tag="ACZPFK_13200"
     CDS             696..1580
                     /locus_tag="ACZPFK_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013106.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1407308.1"
                     /translation="MGDDKKHPDQNGWEPIGPPSIFDEDYVFPGEGQGVGPYQGEEAR
                     EAKRGTKAAAQEKKRRKKEGKAVQKAARGARRAPQPEKAARAAKQKRPRRRRSWKARL
                     VLALLALFVVGAGGLAAYLGVATRNDDLWLDLDQIPYKTGTVIYAREEGGEWEEYAVL
                     PCTQNKEYVPGDQMPQALKDAFIAVEDKDFYTHSGVNLLRTGYAVANELKHALTGTYF
                     GGEDGIKVGASTIDQQLVKNLTRDDDAGGLEGYLRKFREIWRAFRLDASYDKETILEA
                     YLNTISFTDNTAGVQDCR"
BASE COUNT          468 a          402 c          466 g          270 t
ORIGIN      
        1 ggtttcccga taaacatcaa tcgaataacc caacacctga aaagtataaa agctgattcc
       61 caccggcagc aatagagaaa gttgagccgg aacaagtgtt atacccaaaa gtgcaaaaac
      121 acgaacggcc agggcggaaa acatattata atatttgaaa aggaataaaa gacccagatt
      181 tagcagaatc gtgacaatca aaatcatctt gcgggccctt gatgcattga tccgctccaa
      241 gagacgcccg caaaagtagg tggtaagagt ggaaaaaagc aaaaagagcg agtaaactat
      301 gtcaaagcac acgtagaaat aataagacat caccagaagc caactgtttt tcagcttctg
      361 tggaacgaca taatagccca gcgcggcaac aggaaaaaac aagataaaag aaagggatgt
      421 aaaagccata agaaaccctc ttttgtcgac aaaatagatg ataatattta attttaggaa
      481 gaattaacga actatattat atagtaataa aagaaaaaag tctattgtaa tccgcttgct
      541 gtgtgaattg gcaaaaagca cgcaaaaaaa tgctgtttaa ctttttcaaa tcgcgcaaaa
      601 tttacaaaat aaccgcgatt tttgagcggg acggcggtaa aatagggttt gaaacggatc
      661 gcgcgccgat cgcgcgggga gggaggacaa gcctcatggg cgatgataaa aagcatccgg
      721 accaaaacgg ctgggagccg atcgggccgc ccagcatttt tgacgaggac tacgtcttcc
      781 ccggcgaggg acagggtgtc ggcccttatc agggcgagga ggcccgggaa gcaaaaaggg
      841 ggacaaaggc cgcggcgcag gagaaaaagc ggcgaaaaaa agagggcaag gccgtccaaa
      901 aggccgcgcg cggcgcgcgg cgcgccccgc agccggaaaa agcggcccgg gcggcaaagc
      961 aaaagcgccc gcgccgccgc cgctcgtgga aagcgcgcct ggtgctggcg ctgctggccc
     1021 tgtttgtggt gggggccggg ggccttgccg cctacctcgg cgtcgccacc cgcaacgacg
     1081 acctttggct ggacctcgat cagatcccct ataagaccgg cacggtcatc tatgcgcggg
     1141 aagagggcgg ggagtgggag gaatacgcgg ttctgccctg cacccagaac aaggaatacg
     1201 tgcccggcga ccagatgccc caggcgctga aggacgcgtt catcgccgtg gaggacaagg
     1261 atttttacac ccacagtggc gtgaacctgc tgcgcaccgg ctatgcggtg gcaaacgagc
     1321 tcaagcacgc cctcaccggc acctatttcg gcggcgagga cggcatcaag gtgggggcct
     1381 ccaccatcga ccagcagctg gtaaaaaacc tgacgcggga cgacgacgcc ggggggctgg
     1441 agggctatct gcgcaagttc cgcgagatct ggcgtgcttt ccggctggac gcgtcctacg
     1501 ataaggagac aatcctggag gcctacctca acaccatcag ctttaccgac aacacggcgg
     1561 gcgtgcagga ttgtcggtaa agctgatggt gttgaggtag gcctcc
//
LOCUS       DBMDYA010000673         1606 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15074_length_1606_cov_1.457143, whole genome shotgun sequence.
ACCESSION   DBMDYA010000673 DBMDYA010000000
VERSION     DBMDYA010000673.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15074_length_1606_cov_1.457143"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="ACZPFK_13205"
     CDS             complement(<1..228)
                     /locus_tag="ACZPFK_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR1407309.1"
                     /translation="MKILIAVAGKTGTALKCGALLARQLPGAQVADLELERPDPAVYD
                     AVIVGGAVRMGQLHRKARAFLEQNGAALLARP"
     gene            complement(322..>1606)
                     /locus_tag="ACZPFK_13210"
     CDS             complement(322..>1606)
                     /locus_tag="ACZPFK_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1407310.1"
                     /translation="GTAYGKQTLHRGGIWYTAQSGIWQTVWLERVPENYFETVRVTPL
                     YDEAGVRFEPVFGPDCAPLPLEVTVTAEGRAVAAGSAAPGEALTLALPGFHSWSPEDP
                     FLYDFTARAGEDAAQGYFGMRSFGVGKDEAGVPRLLLNGRPYFQNGLLDQGYWSDGLY
                     TAPSDEALVYDIETAKSMGFNLLRKHIKIEPARWYYHCDRLGMLVWQDMVSGGGPYSP
                     MTIQVLPFLGKKLRDDAYGRFGRRDASGRAEADRMRHETVEALYNAVCIALWVPFNEG
                     WGQFDAVRAAREIKAQDPTRLVDHASGWHDQGGPDVCSLHVYFKKVKAGPDPAGRPVV
                     LSEFGGYSYGAPGHTVSPRLFGYRRFDAPERFLAAYRGLIEKEVAPLTGVLSAAVYTQ
                     LSDVEDEVNGLLSYDRRCCKADPAALREINEKLRL"
BASE COUNT          231 a          621 c          507 g          247 t
ORIGIN      
        1 gggccgcgcc agcagcgccg ccccgttctg ctccaaaaag gcccgcgcct tgcggtgcag
       61 ctggcccatg cgcaccgcgc cgcccacgat caccgcgtca tagaccgccg ggtcggggcg
      121 ctccagctcc aggtccgcca cctgcgcccc gggcagctgc cgcgccagca gcgccccgca
      181 cttgagggcc gtaccggtct tgcccgccac cgcgatcaaa attttcatct gcgtttcctc
      241 cccgccgctc tctcgcggcg gccccgcgcg gggccgccga aagaggcgcg tcactttttt
      301 gcccgccgcg cccaaagccc tttacaggcg cagcttttcg ttgatctccc gcagcgcggc
      361 ggggtcggcc ttgcagcagc gccggtcgta gctcagcagg ccgttgacct cgtcctccac
      421 gtccgagagc tgggtgtaga ccgccgccga cagcacgccg gtgagcggag cgacctcctt
      481 ttcgatcagg ccgcgatagg ccgcgagaaa ccgctcgggc gcatcaaagc gccggtagcc
      541 gaacaggcgc ggcgacaccg tgtgccccgg cgccccgtag ctgtagccgc cgaactccga
      601 cagcaccacc ggccgcccgg cggggtccgg cccggctttc acctttttga agtagacgtg
      661 caggctgcac acgtccggcc cgccctggtc gtgccagccg ctggcgtggt ccacaaggcg
      721 ggtggggtcc tgcgccttga tctcccgcgc cgccctcacc gcgtcgaact ggccccagcc
      781 ctcattaaag ggcacccaca gggcgatgca gaccgcgttg tacagcgcct ccaccgtctc
      841 gtggcgcatc cggtcggcct cggcccggcc ggatgcgtcc ctgcgcccaa accgcccata
      901 ggcgtcgtcc cgcagctttt tgcccaaaaa cggcagcacc tggatggtca tggggctgta
      961 gggcccgccg ccgctgacca tgtcctgcca gaccagcatg cccagccggt cgcagtggta
     1021 gtaccagcgg gcgggctcga tcttgatgtg cttgcgcagc aggttgaagc ccatcgactt
     1081 cgcggtctcg atgtcgtata ccagcgcctc gtcggagggc gcggtgtaca ggccgtccga
     1141 ccaatagccc tggtccagca gcccgttctg aaaatagggc ctgccgttga gcagcagccg
     1201 gggcacgccg gcctcgtcct tgcccacgcc gaagctgcgc atgccgaaat agccctgcgc
     1261 cgcgtcctcg cccgcccgcg ccgtgaagtc gtagaggaac gggtcctccg ggctccagct
     1321 gtgaaagccg ggcagcgcca gggtcagcgc ctcgccgggg gccgcgctgc ccgccgccac
     1381 cgcgcgcccc tccgccgtca ccgtgacctc cagcggcagc ggcgcgcagt ccgggccgaa
     1441 caccggctca aagcgcaccc ccgcctcgtc gtacagcggg gtcacccgca ccgtctcgaa
     1501 atagttttcc ggcacccgct ccagccagac cgtctgccag atgccgctct gggcggtgta
     1561 ccagatgccg cccctatgta gggtctgctt gccgtaggcg gtgccg
//
LOCUS       DBMDYA010000674         1600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15137_length_1600_cov_1.598399, whole genome shotgun sequence.
ACCESSION   DBMDYA010000674 DBMDYA010000000
VERSION     DBMDYA010000674.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15137_length_1600_cov_1.598399"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="ACZPFK_13215"
     CDS             complement(<1..813)
                     /locus_tag="ACZPFK_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1407311.1"
                     /translation="MEKTTLSEYLYRTLAAQIRAGRLKSGQPLPSARRLCERYQVGIR
                     TVRDVMARLQREGYIQTRERRAPVVVCLGEPEGEDERAIHSLLSRRESIGQVYETLGA
                     VMPDLFWAGARRSGEEEFRALERLAKKSAQVQPKDSWRLSSAVLHGILAQSRNPLFND
                     LYSSLELYCQVPVFEGHCDPFAKVIAEKKPFTWVIEPLRQGDRAEAELRIGRMYRGTG
                     REVAAYLERLSQEFPGVEQDPQKVYRWNAAKGRTHLYAEIARQLIDEIGRGKW"
     gene            1419..>1600
                     /locus_tag="ACZPFK_13220"
     CDS             1419..>1600
                     /locus_tag="ACZPFK_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407312.1"
                     /translation="MHKATSRRTQNYLRDVSDGAAQLVDTRLLGVLQAMELVADSTWR
                     IPPADRQEFLLRKAQIG"
BASE COUNT          270 a          566 c          432 g          332 t
ORIGIN      
        1 ccatttcccc cgcccgatct cgtcgatcag ctgccgggcg atctccgcat aaagatgggt
       61 gcgccccttg gccgcgttcc agcggtagac cttttgcgga tcctgctcca ccccgggaaa
      121 ctcttgggat aaacgctcca gataggcggc gacctcccgg cccgtgcccc ggtacatccg
      181 cccgatcctg agctcggcct cggcccggtc cccctgccgc agcggctcga tcacccaggt
      241 aaagggcttt ttttcagcga tcaccttggc aaacgggtcg caatggccct caaacaccgg
      301 cacctggcaa tagagctcca agctggaata caggtcgttg aacagcgggt tgcgggactg
      361 agccaagatg ccgtgcagga cggcggagga cagcctccag ctgtcttttg gctgcacctg
      421 ggctgatttt ttcgccagcc tttccagcgc gcgaaattcc tcttcgccgc tccgccgcgc
      481 gcccgcccaa aacaagtcgg gcatcaccgc cccaagggtc tcgtagacct gcccgatgga
      541 ttcccggcgg gagagcagcg agtggatggc ccgctcgtcc tccccttccg gctcgcccag
      601 gcagaccacc accggcgccc ggcgctcccg ggtctggata tagccctccc gctgcaggcg
      661 cgccatcacg tcccgcacgg tgcggatccc cacctgatag cgctcgcaga gccgccgcgc
      721 ggacggcagc ggctgcccgc ttttgagccg cccggcgcgg atctgcgcgg ccagcgtccg
      781 gtaaagatac tccgacaacg tcgtcttttc catcctttcc ctctaccctc tctgctcttt
      841 tcgccctttc cgggcgcggc cgcccgccgc ccggcgagcg gcgttcttct tttcccctcc
      901 gcacagcgcc ctgccgccct cctgcaaggc cctcgccgcg agttgtcggc aggaggcctc
      961 acccgtttcg ccccggccgg gctggaaaca gagtcccccg cagcggcccg aaaccgacgg
     1021 aaagcagacc cgctcccgcg gcacctctca ctagctccca tcggagcaaa cgcccctttg
     1081 cgtcccgcaa gcccgccaaa accgctgctc cgacaatccg cccatcattc taataaaggt
     1141 tctccatagt ataccacgaa aaattttgtt cagctatatg gaaacgtata cagatagcat
     1201 cttgtgaggg atttttcttt ctcttataat caaatcgtgc cattttatga ttttttgtgg
     1261 tttcttttta ttatctgtta cttttgggat aaaggagcac tttgcttatg tcacatctcc
     1321 cccatcggcg ccgtaaacgg ggtgcgctcc tgctgtcagc ggccgctttt ttctttctcg
     1381 ctcttctctc cgccgtcatc ctgtggaaca gccggggatt gcacaaagcc accagccggc
     1441 gcacccaaaa ctatttgagg gatgtctccg acggcgcagc gcagctggtg gacacccgcc
     1501 tgctgggggt cctgcaggca atggagctgg tggcggacag cacctggcgc atcccgcccg
     1561 cggatcggca ggagtttttg ctgcgtaagg cccagatcgg
//
LOCUS       DBMDYA010000675         1593 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15215_length_1593_cov_1.392761, whole genome shotgun sequence.
ACCESSION   DBMDYA010000675 DBMDYA010000000
VERSION     DBMDYA010000675.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_1593_cov_1.392761"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="ACZPFK_13225"
     CDS             <1..560
                     /locus_tag="ACZPFK_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR1407313.1"
                     /translation="PAPSAPQGTPAPSAGGAGQAAPDSSSAAESQPQAVPAPDFTLVD
                     QFGETHTLSDYKGKVVFLNFWATWCGPCQQEMPDIQALYEEHGKNADDLVVLGVANPQ
                     SDDHPAAVDVTEDKVKAFLSDHGYSYPVVMDVTGDLFATYGISAYPTTFMIDAEGNLF
                     GYAQGALTRQIMDDIVEQTMTGRRR"
     gene            767..1006
                     /locus_tag="ACZPFK_13230"
     CDS             767..1006
                     /locus_tag="ACZPFK_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407314.1"
                     /translation="MVSKKKFNTISELQKVQLLATQCPSEVGLHSEDGETIIDAKSYI
                     GMYALDFDRPILVVSEDEDFHKKIEGIGENLPLED"
BASE COUNT          289 a          535 c          503 g          266 t
ORIGIN      
        1 ccccggcgcc ctccgcgccg cagggcacgc ccgccccctc cgccggcgga gccgggcagg
       61 cggccccgga ctcgtcctcc gcggccgagt cccagccgca ggccgtgccc gcgccggatt
      121 tcacgctggt ggatcagttc ggcgagaccc acaccctgtc cgactacaag ggcaaggtgg
      181 tattcctcaa tttctgggcc acctggtgcg ggccctgcca gcaggagatg ccggacatcc
      241 aggcgctgta tgaggagcac ggcaaaaatg cggacgattt ggtggtgctc ggggtggcga
      301 acccccagtc ggacgaccac ccggccgcgg tggacgtgac cgaggacaag gtgaaagcct
      361 ttctctccga ccacggctac agctacccgg tggtgatgga cgtcaccggc gacctcttcg
      421 ccacctacgg catctccgcc tatcccacca cctttatgat cgacgccgag ggcaatcttt
      481 tcggttatgc gcagggcgct ttgacccgcc agatcatgga cgacatcgtg gagcagacca
      541 tgaccgggcg gcggcgctga ccgcggcgtc ccgccgcaca caggggagaa tggcatcttt
      601 caacagttct ccttgttttt gcagcaaaaa tgctttgcat tcccgccccg ccgcgctaaa
      661 ataagactgg gccgggacga atgccgcggg ccccatcgcg cggcggccgc cgcatccgcc
      721 cgttccatgg cccgggaaac cgtgacaaaa gcagaggagt gtttttatgg tcagcaagaa
      781 gaagttcaac accatcagcg agctgcaaaa ggtgcagctc ctcgccaccc agtgcccgtc
      841 cgaggtgggt ctgcactctg aggacggcga gaccatcatc gacgccaaga gctacatcgg
      901 catgtatgcc ctcgactttg accgccccat cctggtggtc agcgaggacg aggattttca
      961 caaaaagatc gagggcatcg gcgagaacct gcccctcgag gactgagctt ttcagcggca
     1021 gggccctgcg ggcgggtgga gaggtctcca tccgcccgca gggctttttt gcccacccgg
     1081 cagctccgcg cccccgggcg cgatcgcaag ccgaaaggcg cgtctcccgc ctgcccccgg
     1141 caagagcgag gcagctttta ctctgcaagg gccgaggggg gcaggaggga gcaggggggg
     1201 ctgcacgccg cggcacaaaa gcggatgtgc ggcccgacct ctcaggtggc tttcggccgc
     1261 cagctcccct gcgcggggcg ccttggaggg tgccctttcg ggcgaagccg caagccgaaa
     1321 ggcaggcgca gcccgctctt tctctgcgag aagcgccatg gaggtcccaa acaccgggcc
     1381 cgcccaagca aaaaagcggc tggggaggac agaaatcctc cccagccgct ttttgttttt
     1441 gtcaaagcac agcgcttgcg cgcagggctc actgctgtgc cgaggcgtcg tctttcaagc
     1501 cgcgcaggct ttccgggtcc ttgcggtaga gcttttcgat ctccttgctg tagcccagca
     1561 gcacatccgg gttggcctcg ttcagcacct gca
//
LOCUS       DBMDYA010000676         1589 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15258_length_1589_cov_1.077957, whole genome shotgun sequence.
ACCESSION   DBMDYA010000676 DBMDYA010000000
VERSION     DBMDYA010000676.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_1589_cov_1.077957"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="ACZPFK_13235"
     CDS             <1..277
                     /locus_tag="ACZPFK_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407315.1"
                     /translation="GLNQYSNQISELEKSTSLSTAQIKQQANALVAAIPGSATLMMRA
                     SMIVLPLALILASYGIYRKYYKIDDKMYRQMIRELEQRADAGEKEND"
     gene            365..>1589
                     /locus_tag="ACZPFK_13240"
     CDS             365..>1589
                     /locus_tag="ACZPFK_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1407316.1"
                     /translation="MEEQKIEAVLKTLTLEEKASLCSGLTSWTTKPVEGKGVPSVFMA
                     DGPTGLRKEDGQHERENGGPSVRATCFPTEATLAGSWDPQLTGAVGEAIGAECRAHGV
                     STLLAPGVNIKRSPLCGRNFEYYSEDPLLAGKLAVGFIRGLRRQGVGCSLKHFAANSE
                     EMLRMSISSEVDERALHEIYLRAFEIAVKEVAPSTVMCSYNRINGVYAADNRELLTGI
                     LREKWGFEGLVMSDWGAVDDRVQGIRAGLDLEMPGSGGVNDLLILNAVKDGSLSEEEL
                     DTTVRRLLRFVFDCVERCVPAPCDYRANHALAVRALEESAVLLKNSGVLPLQEGERIL
                     FVGDMAEHPRYQGGGSSIVNARGLESPLKAARRLGYEVSFARGWPGGDEDREDESLRR
                     EAVRAAADHDKVVLFL"
BASE COUNT          304 a          474 c          562 g          249 t
ORIGIN      
        1 gggcctgaac cagtactcga accagatcag cgagctggaa aaaagcacct cgctctccac
       61 cgcgcagatc aaacagcagg caaacgccct ggtggccgcc atccccggca gcgccacgct
      121 gatgatgcgc gcctcgatga tcgttctgcc gctggcgctc atccttgcaa gttacggcat
      181 ctaccgcaaa tactataaga tcgacgacaa gatgtatcgg cagatgatcc gcgagctgga
      241 acagcgcgcc gatgcgggcg aaaaagaaaa cgactgacgc ggtgcccggc gccgcgccga
      301 caggtcgctg ggtaacgcgg cagagcgctg cggatcgccc tcggcaggaa aggatggagt
      361 ttgaatggag gaacaaaaga tcgaggcggt gctgaagacc ctgacgctgg aagagaaagc
      421 ctcgctctgc tccggcctga cctcctggac taccaagccg gtagagggca agggggtgcc
      481 ctcggtgttc atggcagacg gccccaccgg cctgcgcaag gaggacggac agcacgagag
      541 ggagaacggc ggccccagtg tgcgggcgac ctgcttcccc accgaggcga cgctggccgg
      601 ctcgtgggac ccgcagctca ccggcgcggt gggagaggcc atcggggccg agtgccgcgc
      661 ccacggcgtt tccaccttgc tggcgccggg ggtcaacatc aagcgcagcc cgctctgcgg
      721 ccgtaatttt gagtattact cggaggaccc tctcttggcg ggcaagctgg cggtgggctt
      781 tatccggggc ctgcggcgcc aaggcgtggg ctgcagcctc aagcacttcg ccgccaacag
      841 cgaggagatg ctgcgcatgt ccatcagcag cgaggtggac gagcgggcgc tgcacgagat
      901 ctacctgcgc gcctttgaga tcgccgtcaa ggaggtggcc cccagcaccg tcatgtgctc
      961 gtataaccgc atcaacgggg tctacgccgc cgataaccgg gagctgctca ctggcatttt
     1021 acgggaaaaa tggggctttg agggcctggt gatgagcgac tggggcgcgg tggacgaccg
     1081 ggtgcagggc atccgcgccg ggctggacct ggagatgccg ggcagcggcg gtgtgaacga
     1141 tctgctgatc ctaaacgctg taaaggatgg aagcttgtcg gaagaagagc tggacacgac
     1201 ggtgcggcgc ctgctccgct ttgtgttcga ctgtgtggag cgctgtgtcc cggccccctg
     1261 cgactaccgg gcaaaccatg ccctggcggt gcgcgcgctg gaagaatcgg ccgtgctgct
     1321 gaaaaacagc ggcgttctgc ccctgcaaga gggggagcgg atcctttttg tgggggacat
     1381 ggccgagcat ccccgctacc agggcggcgg cagctccatc gtcaacgccc gcgggctgga
     1441 aagcccgctc aaggcggccc ggcggctggg atacgaggtc tcgtttgccc gcggctggcc
     1501 gggcggcgat gaggaccggg aagatgaaag cctgcggcgg gaggcggtaa gggcggccgc
     1561 ggatcatgac aaggtggtgc tttttctcg
//
LOCUS       DBMDYA010000677         1576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15413_length_1576_cov_1.654237, whole genome shotgun sequence.
ACCESSION   DBMDYA010000677 DBMDYA010000000
VERSION     DBMDYA010000677.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15413_length_1576_cov_1.654237"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="ACZPFK_13245"
     CDS             complement(<1..943)
                     /locus_tag="ACZPFK_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1407317.1"
                     /translation="MDKREFDNPLRQQCMSLPALCADQIAGVRRGLENALTVEELRQI
                     RRVIITGCGDSYVAAQAAIPAFKKFAGKFGSNFSYGRAIEVARYLPFEEKQSPYTLVV
                     GVSCSGGPARVQEVLRRANHYGCMTLAVTNNPDSPAAQEAKRSLIVNTPAFPNASPGL
                     RNYYASLVGLYLLAASLGEATGCSAPGAVDGMADAIAAYTAAYEPLLAGYDEQMFRLA
                     QSWKEFKAYDFIGDDIQFCTAFFTAAKIVEVAGGMTFTDDSEDWCHVGFFQKEPHRIG
                     TTVVADRTANDRSRIGETIKQAAGIGRPVLLVANGGRE"
     gene            complement(962..>1576)
                     /locus_tag="ACZPFK_13250"
     CDS             complement(962..>1576)
                     /locus_tag="ACZPFK_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR1407318.1"
                     /translation="DRNIPTRVSYKHQDSPYDASGHVKSAMIGSSATLIIHEGKLVLG
                     SSQGLVFVEFDGPRPREFYVQVIEKPLAIEKVNVGTVYMGMHDITGEVRDVVRRSGVK
                     DGICHVSVLHSTAGLVLAPADEASRKDVMEDIERMVPTRVDFKHRETASDAGGHVKTA
                     LTGTQLTLPVCGGELALGEGQAVVFAEFDGPRPRSCFVAVYAGC"
BASE COUNT          255 a          522 c          529 g          270 t
ORIGIN      
        1 cctccctgcc gccgttggcc accagcagca cgggccggcc gatgcccgcc gcctgcttga
       61 tggtctcgcc gatgcggctg cggtcgttgg cggtgcggtc cgccacgacg gtggtgccga
      121 tgcggtgcgg ctctttctgg aaaaagccca catggcacca gtcctcggag tcgtcggtaa
      181 aggtcatgcc gccggccacc tccacgatct tggccgcggt gaagaacgcg gtgcagaact
      241 ggatgtcgtc gccgatgaaa tcataggcct tgaactcttt ccagctctgg gccagccgga
      301 acatctgctc gtcataaccc gccagcagcg gctcataggc cgcagtgtag gcggcgatgg
      361 cgtccgccat gccgtccacg gccccggggg cgctgcagcc ggtggcctcg cccaggctgg
      421 ccgccagcag gtacaacccc accagagagg catagtagtt gcgcaggccg gggctggcgt
      481 tggggaacgc cggggtgttg acgatgaggc tgcgcttcgc ctcctgtgcc gcgggggaat
      541 ccgggttgtt ggtgaccgcc agggtcatgc agccgtagtg gttggcgcgg cgcagcacct
      601 cctgcacgcg ggcgggcccg ccggaacagc tgacccccac caccagggta taggggctct
      661 gcttctcttc aaagggcaga tagcgggcca cctcaatggc gcggccatag ctgaagttgg
      721 agccgaactt gcccgcaaat tttttaaacg cggggatggc ggcctgggcg gccacatacg
      781 agtcgccgca gccggtgatg ataacacggc ggatctggcg cagctcctcc accgtcaggg
      841 cgttctcgag gccgcggcgc acgccggcga tctggtccgc gcagagcgcg ggcaggctca
      901 tgcactgctg gcgcagggga ttgtcgaatt ctctcttatc catttgttct ccctccttga
      961 atcagcagcc ggcgtagacg gcgacaaaac agctgcgggg ccgcgggccg tcgaactcgg
     1021 cgaacaccac cgcctgcccc tcgcccagcg ccagctcgcc gccgcacacg ggcagggtca
     1081 gctgggtgcc ggtgagggcg gtctttacat ggccgcccgc gtcggacgcg gtctcgcggt
     1141 gcttgaagtc cacccgggtg ggcaccatgc gctcgatgtc ctccatcacg tccttgcggg
     1201 acgcctcgtc cgccggcgcc agcaccagcc cggcggtgga gtgcagcacc gacacatggc
     1261 agatgccgtc cttgacgccg ctgcggcgca ccacgtcccg cacctcgccg gtgatgtcgt
     1321 gcatgcccat ataaacggtg cccacgttga ccttttcgat ggccagcggc ttttcgatga
     1381 cctgcacata gaactcgcgc gggcggggcc cgtcaaactc cacaaagacc agcccctggg
     1441 acgagcccag caccagcttg ccctcatgga tgataagggt ggccgaggaa ccgatcatcg
     1501 ccgatttgac gtggccggag gcgtcgtagg gggagtcctg atgcttgtag ctgacacggg
     1561 tggggatatt gcggtc
//
LOCUS       DBMDYA010000678         1576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15416_length_1576_cov_1.164068, whole genome shotgun sequence.
ACCESSION   DBMDYA010000678 DBMDYA010000000
VERSION     DBMDYA010000678.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15416_length_1576_cov_1.164068"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="lepA"
                     /locus_tag="ACZPFK_13255"
     CDS             <1..1501
                     /gene="lepA"
                     /locus_tag="ACZPFK_13255"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1407319.1"
                     /translation="ILIVDATQGVEAQTLANTYMALEHDLEILPVLNKIDLPSAEPDR
                     VAREVEEEIGLPCLDAPRISAKTGENVEAVLEAIVQGIPAPVGDENAPLQALIFDAVY
                     DSYKGVIVYVRLREGRVRPGDRIRMMQTGAEFDVVEVGRMGALSLAPCDGLEAGEVGY
                     ITASIKSLRETRVGDTVTGADRPAAEPLPGYRRVVPMVFCGIYPADGARYNDLREALD
                     KLQLNDASLSFEPETSAALGFGFRCGFLGLLHLEIIIERLEREFDLDLVTTAPSVQYR
                     ITLTDGTVKLISNPTDYPDPATIKSAEEPFVNGHIYTPTEYVGSLMELCQNRRGVFRD
                     MKYLDATRVDLHYELPLGEIVYDFFDAIKSLSRGYASYDYDVKGYVESKLVRLDILIN
                     GDPVDALSLIVHTDKAYERGRKICEKLKDNIPRQLFEVPIQAAIGGKIIARETVRALR
                     KDVLAKCYGGDITRKKKLLEKQKEGKKKMRQIGTVEIPSEAFTAVLKLD"
BASE COUNT          299 a          508 c          493 g          276 t
ORIGIN      
        1 catcctcatc gtggacgcca cccagggggt ggaggcccag accctggcca acacctatat
       61 ggcgctggag catgaccttg aaatcctgcc cgtgctcaac aagatcgacc tgccctccgc
      121 cgagccggac cgggtcgccc gcgaggtgga ggaggagatc ggtctcccct gcctggacgc
      181 gccgcgcatc tcggccaaaa ccggcgagaa cgtggaggcg gtgctggagg ccatcgtcca
      241 gggcatcccg gcccccgtgg gggacgaaaa cgcgccgctg caggccttga tcttcgacgc
      301 ggtctacgac agctacaagg gcgtcatcgt ctatgtgcgg ctgcgggagg gccgtgtgcg
      361 gcccggcgac cgcatccgca tgatgcagac cggggctgag ttcgacgtgg tggaggtggg
      421 acggatgggc gctctctccc tggccccctg cgacgggctg gaggcgggcg aggtgggcta
      481 tatcaccgcc tccatcaaga gcctgcggga gacccgggtg ggcgacacgg tcaccggcgc
      541 cgaccgcccc gccgctgagc cgctgcccgg ctatcgccgg gtggtgccga tggtcttctg
      601 cggcatctat cccgccgacg gcgcccgcta caacgatctg cgcgaggcgc tggacaagct
      661 gcagctcaac gacgcctccc tgagctttga gcccgagacc tcggcggcgc tgggctttgg
      721 tttccgctgc ggttttctgg ggctgctgca cctggagatc atcatcgagc ggctggagcg
      781 ggagttcgat ctggatcttg tcaccaccgc ccccagcgtg cagtaccgca tcaccctcac
      841 cgacggcacg gtaaagctga tctccaaccc aaccgattac cctgaccccg ccaccatcaa
      901 gtcggcggag gagccctttg tcaacggcca catctacacc cccaccgagt atgtgggcag
      961 cttgatggag ctgtgccaga accggcgcgg cgttttccgc gacatgaaat atctggacgc
     1021 aacccgggtg gacctgcact atgagctgcc gctgggcgag atcgtctacg actttttcga
     1081 cgccatcaag agcctgtccc gcggctatgc cagctatgac tacgacgtca agggctatgt
     1141 ggaatccaag ctggtgcggc tggatatcct gatcaacggc gacccggtag acgccctgag
     1201 cctgatcgtc cacaccgaca aggcttatga gcgcggccgc aagatctgtg aaaagctgaa
     1261 agacaatatc ccccgccagc tctttgaggt gcccatccag gcggccatcg gcggcaagat
     1321 catcgcccgc gagacagtgc gcgctctgcg caaggacgtg ctggccaaat gctacggcgg
     1381 cgacatcacc cgcaaaaaga agctgctgga aaagcagaaa gagggcaaaa agaagatgcg
     1441 ccagatcggc acggtggaga tccccagcga agcgttcacg gccgtcttga agctggattg
     1501 agccgatctc cccgttttgg gtcccaagct ctcttttcta tttataaata ccccggcttt
     1561 gccggggctt ttttat
//
LOCUS       DBMDYA010000679         1575 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15434_length_1575_cov_1.154003, whole genome shotgun sequence.
ACCESSION   DBMDYA010000679 DBMDYA010000000
VERSION     DBMDYA010000679.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15434_length_1575_cov_1.154003"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(58..549)
                     /locus_tag="ACZPFK_13260"
     CDS             complement(58..549)
                     /locus_tag="ACZPFK_13260"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1407320.1"
                     /translation="MARRDKTNYYLDIADAVSRRGTCLRRNYGAIIVKNDEILSTGYV
                     GAPRGRKNCSDMGVCIREKLQIPRGERYELCRSVHAEANAIISAERKSMIGATLYLSG
                     RNVADGRYVENAMCCSMCKRMVINAGISTVVVRVDDEHYRTVDVQAEWVAQDESLDGV
                     MGC"
     gene            851..>1575
                     /gene="rsmA"
                     /locus_tag="ACZPFK_13265"
     CDS             851..>1575
                     /gene="rsmA"
                     /locus_tag="ACZPFK_13265"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1407321.1"
                     /translation="MPNLTDLKTIRALCERHGFSLSKGFGQNFIVNPGICPKMVEAAG
                     IDRTFGVLEIGPGVGVLTKEAALRAAKVVAVEVDERLPPLLAETLAEFDHVKIILGDV
                     LKTDLSALIDREFAGLRVAVVANLPYYITSPILMKLLEERLPIESITAMVQKEAADRI
                     CAAPGSRDAGAISLAVRYYAEPRVLFTVSPGSFTPPPKVTSAVIQLQLRPAPPVTPRD
                     EARMFAVIRAAFSQRRKTAVNAVA"
BASE COUNT          247 a          562 c          478 g          288 t
ORIGIN      
        1 ccaaagcgcg cccggccctg cggccgccct gtcctttttt gtgccgaggc acgcgcctca
       61 gcatcccatc acgccgtcca ggctttcgtc ctgggccacc cactccgcct gcacgtccac
      121 ggtgcggtag tgctcgtcgt cgacgcgcac caccacggtg ctgatgccag cgttgatcac
      181 catgcgcttg cacatgctgc agcacatggc gttctccaca taccgcccgt ccgccacatt
      241 gcggccggag aggtagagcg tggccccgat catcgacttg cgctcggcgc tgatgatggc
      301 gttggcctcc gcgtgcacgc tgcggcagag ctcataccgc tccccgcggg ggatctgcag
      361 cttttcgcgg atacagaccc ccatatcgct gcaatttttg cgcccgcgcg gcgcgcccac
      421 ataaccggtg gagaggatct cgtcgttttt gacgatgatc gcgccgtagt tgcgccgcag
      481 acaggtgccg cgccgcgaga ccgcgtcggc gatgtccaga taataattag tcttgtccct
      541 gcgcgccata gagcaccccg tccccggggc ggattttttg tctgcccgtc ccgttttttt
      601 ctatgataaa cgattgcggc caaatgtgca agccttgccc gagaggagta acccgtcgcc
      661 ccacctcccg cgcgaaaggc gcaccccggg gcctcggcgc ccgcgtgccc cccttgcgcc
      721 gcaaagcgcg ccgccgggcg ggcttttttc ttttgggccc gctttgctgc gccgtggggc
      781 ggatttatgg tatactggac acagagcggg cgccccgcgc cccaaaacgc tggaaatgga
      841 gctttccctc atgccgaacc tcaccgatct caagaccatc cgcgcgctct gtgagcgcca
      901 cggcttttcc ctctccaagg gctttggaca aaatttcatc gtcaaccccg gcatctgccc
      961 caaaatggtg gaggccgccg gcattgaccg caccttcggc gtgctggaga tcggccccgg
     1021 cgtgggggtg ctcaccaagg aggccgccct gcgggccgcc aaggtggtcg ccgtggaggt
     1081 ggacgagcgc ctgccgcctc tgctggccga gacgctggcg gaattcgatc acgtgaagat
     1141 cattttgggc gacgtgctga agaccgacct ctccgcgctg atcgaccgcg aatttgcagg
     1201 gctgcgggtg gcggtggtgg ccaacctgcc ctattacatc accagcccca tcctgatgaa
     1261 gctgctggaa gagcgcttgc ccatcgagag catcaccgcc atggtgcaaa aggaggcggc
     1321 ggaccgcatc tgcgccgccc ccggcagccg ggacgccggc gccatcagcc tggccgtgcg
     1381 ctattacgcc gagcccaggg tgctgttcac ggtttcaccc ggcagcttca cgccgccgcc
     1441 caaggtcacc agcgccgtga tccagctgca gctgcgcccc gcgccccccg tgacgccgcg
     1501 ggacgaggcg cggatgttcg ccgtcatccg cgccgctttc agccagcgcc gcaaaacggc
     1561 cgtcaacgcc gttgc
//
LOCUS       DBMDYA010000680         1575 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15437_length_1575_cov_0.926052, whole genome shotgun sequence.
ACCESSION   DBMDYA010000680 DBMDYA010000000
VERSION     DBMDYA010000680.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15437_length_1575_cov_0.926052"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="ACZPFK_13270"
     CDS             <1..1103
                     /locus_tag="ACZPFK_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407322.1"
                     /translation="VIMVLCVGGGGQKMMSDELGALASGSVYISVGGTDTTESDYFTD
                     ADVEAVQGLEGVRGVTMAGGASGTVRGTKEPVSASISAGNGDTSLVFPVKLQAGRFWD
                     AGDYEAARRVVTVDSDGAKALFGTDNVLGMTVELTVGGRTSDFTIVGITESHSGYSYN
                     GQVTAQMTVPLSSLSTLSDDVGGPYFQIALLARDPADSVRLARRVVSLLEGRHGNAGR
                     DCYSIMDVSQYTSQINSVTGAFTGIIAAIAGISLLVGGIGVMNIMLVSVTERTREIGI
                     RKALGAKTGSITFQFLIEAGTLTLIGGVIGILLGVWGGNALSGLMGLQGYIAPSMILA
                     IALFSVGIGIFFGIYPARKAAKLSPIEALRTE"
     gene            complement(1263..>1575)
                     /locus_tag="ACZPFK_13275"
     CDS             complement(1263..>1575)
                     /locus_tag="ACZPFK_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407323.1"
                     /translation="RLAGASRQERAAVAAAARRTLLGAPYNIAVGIFPAKGDGTGVTS
                     THCAHLVWSAYRAFGYDLDATGGPVVTPRDLLRSPLLEVVQVYGLDPQALLRERADFC
                     P"
BASE COUNT          254 a          493 c          587 g          241 t
ORIGIN      
        1 cggtgatcat ggtgctctgc gtgggcgggg gcggccagaa gatgatgagc gatgagctgg
       61 gcgcgctggc cagcggctcg gtgtatatca gcgtgggcgg cacggacacc accgagagcg
      121 attatttcac cgacgcggat gtggaagcgg tccaggggct ggagggggtg cggggcgtga
      181 cgatggccgg cggggccagc ggcacggtgc gcggcaccaa agagccggtc agcgccagca
      241 tttccgccgg caacggggac acctccctgg tctttcccgt caagctgcag gcgggccgct
      301 tttgggacgc cggggactac gaggcggcgc ggcgggtggt gaccgtggat tccgacggtg
      361 caaaggcctt gttcggcacc gacaacgtgc tgggcatgac ggtggagctc accgtgggcg
      421 gccgcacctc ggatttcacc atcgtgggca tcaccgagag ccatagcggc tacagctaca
      481 acggccaggt caccgcacag atgacggtgc cgctgtccag cctgtccacc ctctcggacg
      541 acgtgggcgg cccctatttc cagatcgccc tgctggcccg ggatccggcg gacagtgtgc
      601 ggctggcccg gcgggtggtg agcctgctgg aggggcggca cggcaacgcg gggcgcgact
      661 gctacagcat catggatgtg agccagtaca ccagccagat caacagcgtc accggcgcct
      721 ttaccggcat catcgcggcc atcgcgggca tcagcctgct ggtgggaggc atcggcgtga
      781 tgaacatcat gctggtctcg gtcaccgagc gcacgcggga gatcggcatc cgcaaggcgc
      841 tgggtgccaa gacgggcagc atcacgttcc agttcctcat cgaggcgggc accctcaccc
      901 tgatcggcgg ggtcatcggc atcctgctgg gggtctgggg cggcaatgcg ctgagcggcc
      961 tgatggggct gcagggctat atcgccccct ccatgatcct cgccatcgcc ctcttctcgg
     1021 tgggtatcgg catctttttc ggcatctacc cggcgcgcaa ggcggcaaag ctgtcgccca
     1081 tcgaggcgct gcggaccgag tgaattttca acaaaaagcc ctctggggcc cggtagaagc
     1141 cgggccccag gggctttttt gtttttgtca aagcgcgggg agggtgaggg tacaaagcgc
     1201 gggcttcaag ggcgggccgg gggacgggcc ggatggccga aaaacggggc tcagggcggc
     1261 cctcaggggc aaaagtcggc ccgctcgcgc agcagcgcct gcgggtccag gccgtagacc
     1321 tgcaccacct ccagcagcgg gctgcgcagc aggtcgcggg gggtcaccac ggggccgccg
     1381 gtggcgtcca ggtcgtagcc gaacgcccgg taggcggacc agaccaggtg ggcgcagtgg
     1441 gtggaggtca cgccggtgcc gtcccccttt gccggaaaga tgcccaccgc aatgttgtag
     1501 ggcgcaccga gaagcgtgcg ccttgcggcc gcggcgacgg cggcccgctc ctgccgcgag
     1561 gcgcccgcca ggcgc
//
LOCUS       DBMDYA010000681         1573 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15463_length_1573_cov_1.359375, whole genome shotgun sequence.
ACCESSION   DBMDYA010000681 DBMDYA010000000
VERSION     DBMDYA010000681.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15463_length_1573_cov_1.359375"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="ACZPFK_13280"
     CDS             complement(<1..1544)
                     /locus_tag="ACZPFK_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR1407324.1"
                     /translation="MPVKVKLKIDHKNEVISLPAIALRGLVVFPNNVIHFEVGRPQSI
                     AAVEWAMENNTSLFLVTQKDMDQEEPKLGDLHPYGVVAEIKQALRVSDDLVKVLVEGK
                     FRARLLEMDTASHFMQAAVKPAPVRGAKSADSVQVEALVRSIREAFEEYLGLNSRIPK
                     DVVYTILSSDDPVFLSEYVPSNLLFKYTDKQRILNESTVLGRLETLLSVFSKENKVLS
                     IEKEIQDKVSVQMDKNQRDYFLREQMRAISDELDEEEDTRREADGYKERIEKLLLPDE
                     AREKLNKEADRLYKMQGNSQEASVIRTYLDTCLDLPWDKSTPENIDINKAEAILNRDH
                     YGLKKVKERILEILAVRKLSDSVKSQIICLVGPPGVGKTSIANSIARCLGRKYARVSL
                     GGVRDEAEIRGHRRTYIGAMPGKLISAVATAKSNNPVLLLDEVDKMAADFRGDPSAAL
                     LEALDPEQDVAFKDLYLDIPFDLSKVLFITTANTTDTIPRPLLDRMDVIELPSYTRVE
                     KFNIAKR"
BASE COUNT          282 a          466 c          473 g          352 t
ORIGIN      
        1 cgctttgcga tgttgaattt ttccacccgg gtgtagctgg gcagttcgat gacgtccatg
       61 cggtccagca gcggcctggg gatggtgtcg gtggtgttgg cggtggtgat gaacagcacc
      121 ttgctcagat caaaggggat gtccagatag aggtccttaa aggcgacgtc ctgctccggg
      181 tccagggcct ccagcagcgc cgcggaggga tcaccgcgaa aatccgcggc catcttgtcc
      241 acctcgtcga gcagaagcac cgggttgttg ctcttcgcgg tggcgacggc gctgatcagc
      301 ttgcccggca tggccccgat ataggtgcgg cggtgcccgc ggatctcagc ctcatcccgc
      361 acgccgccca gcgagacgcg ggcgtatttg cgccccaggc agcgggcgat agaattggcg
      421 atcgaggtct tgcccacgcc cggggggccc accaggcaga tgatctggct ttttaccgaa
      481 tcggagagct tgcggaccgc caaaatctcc agaatgcgct ctttgacctt tttcaggccg
      541 tagtgatccc ggttgaggat cgcctccgcc ttgttgatat caatgttttc cggcgtggac
      601 ttgtcccacg gcaggtcaag gcaggtgtca agataggtgc ggatgaccga cgcctcctgg
      661 gagttgccct gcatcttgta caggcggtcc gcctctttgt tcagcttttc ccgcgcctcg
      721 tcgggcagca gcagcttttc gatgcgctct ttatagccgt cggcctcgcg gcgggtgtcc
      781 tcctcctcgt ccagctcgtc ggagatggcg cgcatctgct cgcgcagaaa ataatcgcgc
      841 tggtttttgt ccatctgcac cgagaccttg tcctgaatct ctttttcaat ggagaggacc
      901 ttgttctcct tgctgaagac cgagagcagg gtttccaggc gacccaacac cgtgctctcg
      961 ttcaggatac gctgcttgtc ggtgtatttg aaaagcaggt tgctggggac atattcactg
     1021 agaaagaccg ggtcgtcact ggacaggatg gtgtagacca catccttggg gatgcgggag
     1081 ttgaggccca gatattcttc aaacgcctcc cggatcgagc gcaccagcgc ctctacctgc
     1141 acgctgtcgg cggacttggc gccccgcacg ggcgcgggct tgaccgcggc ctgcataaag
     1201 tgagaggcgg tatccatctc caacaggcgg gcgcggaact tgccctccac cagcaccttc
     1261 accaggtcgt ccgagacccg cagcgcctgc ttgatctccg cgaccacgcc gtaggggtgc
     1321 aggtcgccca gcttgggctc ttcttgatcc atatcctttt gggtcaccaa aaaaaggctg
     1381 gtattgtttt ccatcgccca ctccaccgcg gcgatggact gggggcgccc cacctcaaag
     1441 tggatgacgt tgttgggaaa caccaccagc ccgcgcagag caatggcggg cagtgaaatg
     1501 acttcgtttt tgtggtctat tttgagcttt accttgaccg gcataaagca gttcctccct
     1561 gatagaaaat cag
//
LOCUS       DBMDYA010000682         1568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15538_length_1568_cov_1.365372, whole genome shotgun sequence.
ACCESSION   DBMDYA010000682 DBMDYA010000000
VERSION     DBMDYA010000682.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15538_length_1568_cov_1.365372"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(361..>1568)
                     /locus_tag="ACZPFK_13285"
     CDS             complement(361..>1568)
                     /locus_tag="ACZPFK_13285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1407325.1"
                     /translation="AGGYQAESAGGPIPEVRYSAVPPKKPKKKGKKIALVATLVSLGM
                     VCTIALSMVAGGMLVKHFTLSGSSSVSQSQPEHLIINGTPAVGDGSSAEATDTGFTTT
                     QIAAKAMSSVVGINLYSPQQVEKVGEASGIVLDKNGYIITNAHVVADGYSPTVVLSDG
                     TEVPATVVGYDTRTDLAVVKVNPSGLELTPAEFGDSDSLQLGDDVVAIGNANGYYNSL
                     SKGVVSGLNREVSTNISLKLIQTDAAINPGNSGGALLNKYAQVVGINSSKLAGSEIDS
                     MGFAIPINDAKPIIDSLINYGYVKDRVALGVTVVALNGTNGAMMGLPSQGLYITQISP
                     DSDLYAQGINRGDVILTAGGVTLNKNSDLLKELENYKPGDTIELTIQKGTGETVTIHA
                     KLHEMTSNS"
BASE COUNT          233 a          466 c          543 g          326 t
ORIGIN      
        1 gctgctcgtg atcgagcgcg tccttgacgt aaagcaattc tttcggggtc ataatttcat
       61 ttcacctctt cttgctgaat gcgggcgccg ggcgcggcgg cagtttgcgg cgcgcctctc
      121 ccgttccctg ctagtgtgcg cagttgccgg cggggtatgc gggggcaaaa gaagaagaat
      181 ttggcggcgg cgcggccctc tccccggccc ggcctccttg ccggggcggc cgctgccccg
      241 cgggcgcggc aggtattggt tccgttcggc tgccgtcaaa aaaaaagaaa gccccggggg
      301 gctttctttt tggtataagg aaaaataagg agcacgcgcg ccggtaagcg gcgccggggt
      361 tcagctgttg gaggtcatct cgtgcagctt ggcgtggatg gtcaccgtct cgcccgtccc
      421 tttctggatg gtcagctcga tggtgtcccc gggcttgtag ttctccagct ctttcagcag
      481 gtcgctgttc ttgttcaggg tcacgccgcc cgcggtcaaa atcacgtccc cgcggttgat
      541 gccctgggcg tacaggtcgc tgtccgggct gatctgggtg atatagaggc cctggctggg
      601 caggcccatc atggcgccgt tggtgccgtt cagggccact accgtcacgc ccagcgccac
      661 ccggtccttg acatagccgt agttgatcag gctgtcgatg atgggcttgg cgtcgttgat
      721 ggggatggcg aagcccatcg agtcgatctc agagcccgcc agcttggagg agttgatgcc
      781 gacgacctgg gcgtatttgt tcagcagcgc gccgcccgag ttgcccgggt tgatggccgc
      841 gtcggtctgg atcagcttga gggagatgtt ggtggacacc tcgcggttga ggccggagac
      901 gacgcccttg gacaggctgt tgtaatagcc gttggcgttg ccgatggcca ccacgtcgtc
      961 gcccagctgc aggctgtcgg agtcgccgaa ttccgcgggg gtcagctcca gcccgctggg
     1021 gttgaccttg acgaccgcca ggtcggtgcg ggtgtcgtag cccaccaccg tggcgggcac
     1081 ctcggtgccg tccgacagca ccacggtggg cgaatagccg tcggcgacga catgggcgtt
     1141 ggtgatgatg tagccgttct tatccagaac gatgccgctg gcctcgccca ccttttccac
     1201 ctgctggggg gagtagagat tgatgcccac cacgctgctc atggcctttg ccgcgatctg
     1261 ggtggtggta aagccggtgt cggtggcctc ggccgagctg ccgtccccca ccgccggggt
     1321 gccgttgatg atcaggtgct caggctggga ctgggagacg gaggaggagc cggacagcgt
     1381 gaagtgcttg accagcatac cgcccgccac catcgagagg gcgatggtgc agaccatgcc
     1441 cagcgagacc agcgtggcca caagggcgat ctttttgccc ttttttttcg gctttttggg
     1501 gggcacggcg ctgtagcgca cctccgggat agggccgccg gcggactcgg cctgatagcc
     1561 cccggcag
//
LOCUS       DBMDYA010000683         1561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15625_length_1561_cov_1.802055, whole genome shotgun sequence.
ACCESSION   DBMDYA010000683 DBMDYA010000000
VERSION     DBMDYA010000683.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15625_length_1561_cov_1.802055"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="ACZPFK_13290"
     CDS             complement(<1..736)
                     /locus_tag="ACZPFK_13290"
                     /inference="COORDINATES: protein motif:HMM:NF022811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIR1407326.1"
                     /translation="MKKHKIGIKFALTLLVSLAVGGVLGYGVAMGRDALAQGLDTAQR
                     FLRQNAVWALLLCALAALPTAWAQYAAGRRHAAAALAAGDGGEGNEEAFERADHCYAA
                     AMGAVGVLNVAAFTLFGVGISGFFSARVPAQGFGGLLALTGVFLALVFFCIYLQSLFV
                     RATKELYPEKQGSVLDGKFQKQWYDSCDEAERRQVGEASYRSMQAMGRAILALFVVLT
                     LLGLVLDLGVTPFLVLGALWMVQTASY"
     gene            complement(723..944)
                     /locus_tag="ACZPFK_13295"
     CDS             complement(723..944)
                     /locus_tag="ACZPFK_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1407327.1"
                     /translation="MPLQNRVKEHRARCGLNQQQLGSRVGASRQTISLIERGDYHPSV
                     LLALRIARVFGAPLEEVFYLTGEETDEKA"
BASE COUNT          283 a          544 c          451 g          283 t
ORIGIN      
        1 gatagctggc cgtctgcacc atccacaggg cgccgagcac cagaaaaggc gtcaccccca
       61 gatccagcac cagccccagc agcgtcagca ccacgaacag cgccaggatg gctctgccca
      121 tcgcctgcat cgagcggtag gacgcctcgc ccacctgccg ccgctcggcc tcgtcacagc
      181 tgtcatacca ctgcttttga aatttgccgt ccaacacgct gccctgcttt tcggggtaga
      241 gctcttttgt ggcgcggaca aaaaggcttt gcaggtagat gcagaaaaag accagcgcca
      301 gaaacacccc ggtcagcgcc agcaggccgc cgaagccctg cgccggcacg cgggcgctga
      361 aaaagcccga gatgcccacg ccgaagaggg tgaacgcggc cacattgagc acccccacag
      421 cgcccatcgc cgccgcatag cagtggtcgg cccgctcaaa ggcctcctcg ttcccctctc
      481 cgccatcccc tgcggccagc gccgcagcgg cgtgccgcct gcccgccgca tactgtgccc
      541 aggccgtcgg cagcgcggcc agcgcgcaga gcagcagggc ccagacggca ttctgccgca
      601 aaaagcgctg cgccgtgtca aggccctggg ccagggcgtc ccggcccatc gccacaccgt
      661 agcccagcac cccgcccacc gccaggctga ccagcagcgt gagtgcaaat ttgatgccga
      721 tcttatgctt tttcatctgt ttcctcccct gtcaaataaa agacctcttc cagcggcgcc
      781 ccaaagaccc gggcgatgcg cagcgccagc agcaccgagg ggtgatagtc cccccgctca
      841 atcaggctga tggtctgtcg ggatgccccc acccggctgc ccagctgctg ctggttgagc
      901 ccgcagcgcg cgcggtgctc cttgacgcgg ttttgcagcg gcattttttc ctcctttttc
      961 tcttattgaa aattttattt gtcattttga caattttaat tgtcaataat agaatagcat
     1021 tgacaactaa aattgtcaag tctttttgtc tttcccggtt ctgcaccggc aaaaccggcc
     1081 ccgggccgtg agaaccagtg cttcccgcag gagggccggg caaaaccggt tttttcactg
     1141 gttccgcctc ttggcggccc tgtttttcgc cgctgcggca ctgggaacaa gcggccaaaa
     1201 accgtttttg ccgtgcctcg gacagccttg atcgcccatc cccttttttt ctcctccgca
     1261 ttccccgccc tgcctccccg acgcgtcccc gccgcgctgc ttcccccggc caggggagac
     1321 gggaggggcg gcgtctgcgc agggcctcgg gcatcagata ctgcttcccc cggccagggg
     1381 aggctgcagg ggtgtaaccg gcggaggagc cgccacaggg gatataagga cccttttgtc
     1441 aaaaccgccg gaccgccgcc tgcggtgggg atggagggga aaacggaggc cgcctttcgt
     1501 cgcgggcggt cggggcatcg ccatattttc aggaggggag aaccggccct gcggggaggc
     1561 c
//
LOCUS       DBMDYA010000684         1560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15641_length_1560_cov_1.424263, whole genome shotgun sequence.
ACCESSION   DBMDYA010000684 DBMDYA010000000
VERSION     DBMDYA010000684.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15641_length_1560_cov_1.424263"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="ACZPFK_13300"
     CDS             <1..852
                     /locus_tag="ACZPFK_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1407328.1"
                     /translation="VIRDITPEVEARERLERQAAQQERQARRYDQLFQSVLCGIVQYR
                     VSGQGVVFKNANREAIRIFGYEPEEFWAKKDWDLPSLIAEEDRGPVLKEISSLRQPGD
                     KSPYEYRLLQKDGAPCWIIGSAEVLLDSDGEQIIQSVFLDIDSRKKAEQRSQRLAEQV
                     EASNEILHLALAGTSTCEFYYYPETRECQIPEHTCQVYGCQSHYADMPTSFALDQLEE
                     PYRPAFCEMYERIHGGESTASCEFVGLGGRFWCRVTLSVVHNGGDGQPELVIGIVEDI
                     SRQKEME"
     gene            856..>1560
                     /locus_tag="ACZPFK_13305"
     CDS             856..>1560
                     /locus_tag="ACZPFK_13305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1407329.1"
                     /translation="MEEARTRDGLTGLYSKERGVRLIQDYLAARPAGEHGVLMLLDMD
                     DFEEINQKEGGVFADAILQEVADLLRAETGPENIQIRLGGDEFMLFLKNCDKVGAMQV
                     GPHLAAQVQGILVNTEKEIRLSVSIGMCSTEVVEDYNSLYRCVESTLKYVKEHGKGQA
                     ACYLDTSNELGVFLTQLYTEEHRVNAIRAETPRREDDLVAFALDLLGKSKNLDDAVFL
                     LLSRVGRTYGFDRVSII"
BASE COUNT          311 a          475 c          525 g          249 t
ORIGIN      
        1 gtcatccggg acatcacgcc ggaggtggag gcgcgcgagc gtctggagcg tcaggcggcg
       61 cagcaggagc gccaggcccg gcggtacgac cagctgttcc aatctgtgct ctgcggcatc
      121 gtgcagtacc gcgtgagcgg gcagggggtc gtcttcaaaa acgccaaccg ggaggccatc
      181 cgcatcttcg gctatgagcc ggaggaattt tgggcaaaaa aggactggga cctgccctcc
      241 ctgatcgcag aggaggaccg cggcccggtg ctgaaagaga tcagcagcct gcggcagccg
      301 ggggacaaaa gcccttacga ataccgcttg ctgcaaaaag acggcgcccc ctgctggatc
      361 atcggcagcg ccgaggtgct gctggacagc gacggcgagc agatcatcca gagcgtcttt
      421 ctggacatcg acagccgcaa aaaagccgag cagcgcagcc agcgcctggc ggaacaggtg
      481 gaggcgagca acgagattct gcacctggcg ctggcgggca cctccacctg cgagttctat
      541 tattaccccg agacccggga gtgccagata cccgagcaca cctgccaggt gtacggctgc
      601 caaagccatt acgccgacat gcccacaagc tttgcgctgg atcagctgga agagccctac
      661 cggcccgcgt tctgcgagat gtacgagcgc atccacggcg gcgagagcac cgcgtcctgt
      721 gagtttgtcg gcctgggcgg ccgtttttgg tgccgcgtca ccctctcggt ggtgcacaac
      781 ggcggggacg ggcagcccga gctggtcatc ggcatcgtgg aggatatctc acgccagaaa
      841 gagatggagt aggcgctgga ggaggcccgc acccgcgacg gcctgaccgg cctgtacagc
      901 aaggagcgcg gggtgcggct gatccaggac tatctggccg cccggcccgc cggcgagcac
      961 ggcgtgctga tgctgctgga catggacgac ttcgaggaga tcaaccagaa agagggcggc
     1021 gtctttgcgg acgccatcct gcaggaggtg gccgacctcc tgcgcgcgga gacggggccg
     1081 gaaaatatcc agatccgcct gggcggcgac gagttcatgc tctttctcaa aaactgcgac
     1141 aaggtgggcg ccatgcaggt gggcccgcac ctcgccgccc aggtgcaggg catcctggtg
     1201 aacaccgaaa aggagattcg cctctcggtc agcatcggca tgtgcagcac cgaggtggtg
     1261 gaggactaca attctttgta ccgctgtgtg gagagcacgc tgaaatatgt gaaagagcac
     1321 ggcaaggggc aggcggcctg ttatctcgac acctccaacg agctgggggt ctttttgacc
     1381 cagctctaca ccgaggagca ccgggtgaac gccatccgcg ccgagacgcc ccggcgcgag
     1441 gacgacctgg tcgcctttgc gctggacctg ctgggcaaat caaaaaatct ggacgacgcg
     1501 gtctttctgc tgctctcccg ggtcgggcgg acctatgggt tcgaccgcgt ctccatcatc
//
LOCUS       DBMDYA010000685         1559 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15655_length_1559_cov_2.155693, whole genome shotgun sequence.
ACCESSION   DBMDYA010000685 DBMDYA010000000
VERSION     DBMDYA010000685.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15655_length_1559_cov_2.155693"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="ACZPFK_13310"
     CDS             <1..530
                     /locus_tag="ACZPFK_13310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1407330.1"
                     /translation="GRPGLQVLRGDFLQCPLPPASFDLAYCATAFHWLPPQLAYPRLR
                     GLLRKGGALALFWNHPFPNREEDETNRASRQVYRRYRPSGEEQREFCEADCEKRVDEL
                     RAYGFCDLRTALFHRTRQLTAEDYIRLLNTYSDHRAMPPQKRAAFEREMRAALEAVGG
                     AINIYDTIDLYLARK"
     gene            complement(667..>1559)
                     /locus_tag="ACZPFK_13315"
     CDS             complement(667..>1559)
                     /locus_tag="ACZPFK_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1407331.1"
                     /translation="LMPFNEHCIVFGERHEPMAITHDTFRRIFDFVGQFPHYTCGSNA
                     DLPIVGGSILSHNHFQGGNYAFPMQRARLQQAYRCAGFPDIRIGRIFWPVSTVRCTGR
                     DTGQLMAFADRLLDAWREYSDESVDIRAYTVENGERTPHNTVTPILHHTEEEGYVLDL
                     VPRNNATSPEHPEGIFHPHREIHHIKKENIGLIEVMGLAILPSRLKAQGEAIRQVLTG
                     ARRAQEVKKEAPEHAEWIDFLTAKYGVRMEDAAAEQAVRAEIGAKFETCLEHAGVFKQ
                     DAAGFAAFDRFMASLGCEKI"
BASE COUNT          279 a          462 c          521 g          297 t
ORIGIN      
        1 ccggccgccc gggtcttcag gtgctgcggg gagattttct gcagtgcccc ttgccgccgg
       61 cgtctttcga tctggcctat tgcgccaccg cttttcactg gttgccgccg cagctggcgt
      121 acccccggct ccgcgggctt ctgcgtaagg gcggggcgct ggcgctgttc tggaaccacc
      181 ctttccccaa ccgggaggag gatgagacca accgggccag ccggcaggtt tatcgcagat
      241 accgccccag cggcgaagag cagcgggagt tctgcgaggc ggactgcgaa aaaagggtgg
      301 atgaactgcg cgcttacggc ttttgcgatc tgcgcacggc gctgtttcac cgcacaaggc
      361 agctcacggc ggaggattac atccgtctgc tgaacaccta ttcagaccac cgggcgatgc
      421 cgccgcagaa gcgggcggca tttgagcggg agatgcgggc cgctttggag gccgtcggcg
      481 gggcgatcaa catctatgac accatcgact tgtatttggc ccgaaaatga cggtgcggcc
      541 cggcggccgt gaaagacaaa gtgggcaaag cggcggacaa aaaagcggga ggacgcgaaa
      601 ccaaaggttt cacgtcctcc cgtcctcttg tataaaagtg aaaagcagcg cggccctgcg
      661 ggggattcag attttttcac agcccaggga ggccatgaag cggtcgaaag ccgcaaagcc
      721 cgccgcatcc tgcttgaaga cgcctgcatg ctcgagacag gtctcaaact ttgccccgat
      781 ctcggcgcgc accgcttgtt ctgcggctgc gtcctccatc cggacgccgt actttgcggt
      841 gagaaagtcg atccactcgg cgtgttccgg ggcctccttt ttgacctcct gcgccctgcg
      901 cgcgccggtg agcacctgac ggatcgcctc gccctgggcc ttgagacggc tgggcaggat
      961 ggccaggccc atgacctcga tcaggccgat gttttctttt ttgatgtggt gaatctcccg
     1021 gtgtgggtga aagatgccct ccgggtgctc ggggctggtg gcgttgttgc ggggcaccag
     1081 gtcaagcaca tagccctcct cctcggtgtg gtgcaggatg ggggtgaccg tgttgtgcgg
     1141 cgtccgttcg ccgttttcaa cagtataggc gcggatgtcc acgctctcgt cgctgtactc
     1201 ccgccaggcg tccagcagcc ggtcggcaaa ggccatcagc tgccccgtgt cgcggccggt
     1261 acagcgcact gtagagaccg gccagaagat gcggccgatg cggatatcgg gaaaacccgc
     1321 acagcggtag gcctgctgca gccgggcgcg ctgcatggga aaggcgtagt tgccgccctg
     1381 gaaatggttg tgggacagga tgctgccgcc cacgatgggc aggtcggcgt tgctgccgca
     1441 ggtgtaatgg gggaactggc ccacaaaatc aaagatgcga cggaacgtgt cgtgggtgat
     1501 ggccatgggc tcatgccttt caccgaagac gatgcagtgc tcgttgaaag gcatgagcc
//
LOCUS       DBMDYA010000686         1557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15691_length_1557_cov_1.210165, whole genome shotgun sequence.
ACCESSION   DBMDYA010000686 DBMDYA010000000
VERSION     DBMDYA010000686.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15691_length_1557_cov_1.210165"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="ACZPFK_13320"
     CDS             complement(<1..835)
                     /locus_tag="ACZPFK_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1407332.1"
                     /translation="MTKLPFKIDLGGKTAVVTGAGGVLCGGFAKTLAACGAKVALLDL
                     NLEAAQAVADDIGAEGGTARAYKANVLEREPLEAVAGQVLADLGGCDILINGAGGNNP
                     RATTDKEYYEPGDLDAETKSFFDLDPAGVEFVFNLNFIGTLIPTQVFARQMVEGGGVI
                     VNVSSMNAFTPLTKIPAYSGAKAAVSNFTQWLAVHFARVGIRVNALAPGFFSTKQNAA
                     LLWNPDGSPTARTGKILAATPMGRFGEPADLSGALLYLVSNEASGFVTGTVLPIDGGF
                     SA"
     gene            complement(841..>1557)
                     /locus_tag="ACZPFK_13325"
     CDS             complement(841..>1557)
                     /locus_tag="ACZPFK_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent putative hexonate epimerase"
                     /protein_id="HIR1407333.1"
                     /translation="RMMGRDKTPVRQVFDYAQQHFLAQLPLAYVLTVTTAPEGVIQTH
                     ALLIGSGRGCFEQAVQQAQRYNLNLIDEPFDKVVVLLDGKEFKSTWLGNKSVYRTRMA
                     IRDGGELIVLAPGVDRFGEDPEIDGLIRKYGYCGRERVIELCGTQQDLQQNLSAAAHL
                     IHGSSDGRFKITYCTRLLSREEVEGVCFDYLPYEEAAKRYDPQKLAYGYNTLPDGERV
                     YYIPNPALGLWADRRKFEKA"
BASE COUNT          254 a          508 c          518 g          277 t
ORIGIN      
        1 aggcggagaa gccgccgtcg atgggcagca cggtgccggt cacaaagccg gaggcctcgt
       61 tgctgacgag atacagcaat gcgccggaga ggtctgccgg ctcgccgaag cggcccatgg
      121 gggtcgccgc cagaattttg ccggtgcgcg ccgtggggct gccgtccggg ttccagagca
      181 gcgccgcgtt ctgctttgtg gagaaaaagc ccggcgccag ggcgttgacc cggatgccca
      241 cccgggcaaa gtgcaccgcc agccactggg tgaagttgct caccgccgcc ttggcgccgg
      301 agtaagcggg tatcttggtc agcggggtga aggcgttcat ggacgagaca ttgacgatga
      361 cgccgccccc ctccaccatc tggcgggcaa agacctgcgt ggggatgagg gtgccgataa
      421 agttgagatt gaagacgaac tccacccccg cggggtccag atcgaaaaag gacttcgtct
      481 cggcgtccag gtcgcccggc tcatagtact ccttgtcggt ggtggcgcgc gggttgttgc
      541 cgcccgcgcc gttgatgagg atatcgcagc cgccgagatc tgccagcacc tgccccgcca
      601 ccgcctccag gggctcgcgc tccagcacat tggccttgta ggcgcgcgcc gtgccgccct
      661 cggccccgat gtcgtccgcc acggcctggg ccgcctccag gttcaggtcc agcagggcga
      721 ccttggcccc gcaggccgcc agggtctttg caaagccgcc gcagagcacg ccgcccgcgc
      781 cggtgacgac agcggtcttg ccgccaagat cgattttaaa gggaagtttt gtcatagctc
      841 tcacgctttc tcaaattttc tgcggtccgc ccacaggccc agcgccgggt tggggatgta
      901 gtagacgcgc tccccgtcgg gcagggtgtt atagccatag gccagcttct gcgggtcata
      961 ccgcttcgcg gcctcctcat agggcaggta gtcaaagcag acgccctcca cctcctcgcg
     1021 gctgagcagc cgggtgcagt aggtgatctt gaagcggccg tcgctgctgc cgtggatgag
     1081 atgggcggcg gcggacagat tctgctgcag atcctgttgg gtaccgcaca gctcgatgac
     1141 ccgctcccgg ccgcagtagc cgtatttgcg gatcagcccg tcgatctcgg ggtcctcgcc
     1201 gaagcggtcc accccgggcg ccagcacgat cagctcgccg ccgtcccgga tggccatacg
     1261 ggtgcggtag acggatttgt tgcccagcca ggtgctcttg aactccttgc cgtccagcag
     1321 caccaccacc ttgtcaaagg gctcgtcgat caggttgagg ttgtatcgct gcgcctgctg
     1381 caccgcttgc tcaaagcagc cgcgcccgct gccgatcaag agggcgtggg tctggatcac
     1441 gccctccggg gcggtggtga cggtgagcac ataggcgagc ggcagttgtg cgagaaaatg
     1501 ctgctgcgcg tagtcgaaca cctgccgcac cggggtcttg tcccggccca tcatccg
//
LOCUS       DBMDYA010000687         1557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15693_length_1557_cov_1.153159, whole genome shotgun sequence.
ACCESSION   DBMDYA010000687 DBMDYA010000000
VERSION     DBMDYA010000687.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15693_length_1557_cov_1.153159"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="ACZPFK_13330"
     CDS             complement(<1..952)
                     /locus_tag="ACZPFK_13330"
                     /inference="COORDINATES: protein motif:HMM:NF022702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR1407334.1"
                     /translation="MKKRTLSLLLASALALGALSGCGGPGGGSSGSAPAPTATPQPVT
                     ALDCNPLTGEDKPAGLADGQRPVAVMINNIQTALPQTGIGSADVIYEMETEGGITRMM
                     AVFADYTTMPLVGPVRSSRDQFVQLVLPMNALFVHIGGSTYSTRMLNYYKYQDIDGMY
                     LGTSAFYFDEERYKTKAQEHCWYTSGELVQRGIDTVGSLSTTGAVSPLFRFADKGAAV
                     RLSGGAASALAFRFSDYGDAAFHYNADTGLYLKDIYGAAQVDAATGEQLAFKNVFVLF
                     AGVGHKPEPQLTDFDLSSGSGYYFTGGSYAPVTWSKDGDQA"
     gene            complement(1012..>1557)
                     /locus_tag="ACZPFK_13335"
     CDS             complement(1012..>1557)
                     /locus_tag="ACZPFK_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407335.1"
                     /translation="LHCIKTFEKERIDLAILYAASILESHCETLRDIFMAILQNDKKL
                     LLPVEGVKPEAGYGFTAWVDHNRIIRGNRAMMARHDIELPSMDYENRYTKNGARAAQS
                     NGAHHKRHGAGRKSAGPPPLPLCGSAHPAVPERRAARALRLGGGAPPAKPPAPPLVKI
                     SHLPGPPQRPPAALQPPVLPL"
BASE COUNT          251 a          468 c          553 g          285 t
ORIGIN      
        1 gggcctggtc gccgtccttg ctccaggtga ccggggcgta gctgccgccg gtgaaataat
       61 agccgctgcc ggaggacagg tcgaagtcgg tcagctgagg ctcgggcttg tgccccacgc
      121 ccgcaaacag cacaaagacg tttttgaacg ccagctgctc gccggtggcg gcgtccacct
      181 gggcggcgcc gtagatgtct ttcaggtaca ggccggtgtc ggcattgtag tggaaagccg
      241 cgtcgccgta gtcggaaaaa cggaaagcca gcgccgaggc cgcgccgccg gacagcctga
      301 ccgccgcgcc cttgtccgca aagcggaaca ggggggagac ggcgccggtg gtggacagcg
      361 agcccaccgt gtcgatgccg cgctgcacca gctcgccgct ggtgtaccag cagtgctcct
      421 gcgccttggt cttatagcgc tcctcatcaa aataaaaagc gctggtgccc aggtacatgc
      481 cgtcgatgtc ctgatatttg taatagttca gcatgcgggt ggaataggtg gagccgccga
      541 tgtgcacgaa cagggcgttc atgggcagca cgagctggac gaactggtcg cgggacgagc
      601 gcaccgggcc caccaggggc atggtggtgt agtctgcaaa aaccgccatc atgcgggtga
      661 tgccgccctc ggtctccatc tcgtagatca cgtccgccga cccgatgccg gtctggggca
      721 gggcggtctg gatgttgttg atcatcaccg ccaccgggcg ctgaccgtcc gccaggccgg
      781 cgggcttgtc ctcgccggtg agggggttgc agtccagcgc cgtgaccggc tggggggtgg
      841 cggtgggggc cggcgcgctg ccggaggagc cgccgcccgg ccccccgcag cccgacaggg
      901 cccccagagc cagcgccgac gccagcagca gcgaaagtgt gcgttttttc atggtttgtc
      961 tcctgacttt atcagaacgg ccgcagccgc gcgtttgtta tatacccctt attataaggg
     1021 caaaacggga ggttgtaaag ccgcaggggg gcgctggggc ggccccggca gatgtgaaat
     1081 tttaaccagc ggcggagcgg gcggttttgc cggtggcgcg ccgcccccca agcggagcgc
     1141 gcgggcggca cggcgctcgg ggacggcggg atgcgcggac ccgcagaggg ggagcggggg
     1201 gggaccggcg cttttgcggc ccgccccgtg ccttttgtgg tgcgcgccgt tgctttgggc
     1261 cgcccgcgcg ccgtttttgg tgtagcggtt ctcgtaatcc atactgggca gttcgatgtc
     1321 gtggcgcgcc atcatggcgc ggttgccccg aatgatgcgg ttgtggtcca cccatgcggt
     1381 gaagccgtag cctgcctccg gcttcacgcc ctctacgggc agcagaagct ttttgtcgtt
     1441 ctgcagaatg gccatgaaga tgtcgcgcag cgtctcgcag tgcgattcca gaatactggc
     1501 ggcgtacagg atggcaaggt cgatgcgctc tttttcaaat gtcttgatgc agtgcag
//
LOCUS       DBMDYA010000688         1555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15712_length_1555_cov_1.701513, whole genome shotgun sequence.
ACCESSION   DBMDYA010000688 DBMDYA010000000
VERSION     DBMDYA010000688.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15712_length_1555_cov_1.701513"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(88..831)
                     /gene="udp"
                     /locus_tag="ACZPFK_13340"
     CDS             complement(88..831)
                     /gene="udp"
                     /locus_tag="ACZPFK_13340"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1407336.1"
                     /translation="MYALNDNGQQYHVELSKGDVGRYVILPGDPGRVERIAKFLDNPR
                     RVACHREYVTYTGSLDGVPVSVTSTGIGGPSTAIAVEELIAVGADTFVRIGTAGMVQD
                     YFDLNDCIIATGSVRDEGTTRQYIPLSYPAVAHYDVVDALRRAAKRCGKTAHVGIVQS
                     KDAFYGEAEPESMPNEPVLRYQWDAWKRGGVLASEMESAALFVVSSIRRVRAGCILNN
                     RGEMDETIRVAVEAIRELIAADRQAQPER"
     gene            complement(860..>1555)
                     /locus_tag="ACZPFK_13345"
     CDS             complement(860..>1555)
                     /locus_tag="ACZPFK_13345"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1407337.1"
                     /translation="IHGRDGMGDLGLPPPALRPQREHAVDFLIRAIEAEPGELEMVTL
                     GPVTNLAWISLRSPGTLAKLKRITMMMGTGPYFGNATPLAEGNARMDPEALDIVLRDA
                     GTELVLVGWDLCINEYLFTEKDLEGLRQTGSPLAAFCLDINHNLVELNERRFGAPVLD
                     FADPVAMAVALRPELVEQSVTAYTRAETNRGLAYGAIAVDHCHQSGKPPNATTCTKLH
                     AEELKRCIISRIV"
BASE COUNT          256 a          541 c          468 g          290 t
ORIGIN      
        1 ggcgacgata ggacgatggg gcatgggcgc aaaacgcagc actacaaaaa gcagggatgc
       61 agacggcatc cctgcttttt ttcgtcccta tcgctccggc tgcgcctgcc ggtccgcggc
      121 gatcagctcg cggatggcct ccaccgccac ccggatcgtc tcatccatct ccccgcggtt
      181 gttgaggatg cagcccgccc gcaccctgcg gatggagctg accacaaaaa gggcggcgga
      241 ctccatctcg ctggccagta ccccgccccg tttccaggcg tcccactgat agcgcagcac
      301 cggttcattg ggcatgctct ccggctccgc ctcgccgtaa aaagcgtctt ttgactggac
      361 aatccccaca tgggcggtct tgccgcagcg ctttgcggcg cggcgcagcg cgtccaccac
      421 atcataatgg gccacggccg ggtaggacag ggggatgtac tgccgggtcg tgccctcatc
      481 ccgcaccgag ccggtggcga tgatgcaatc gttgaggtca aagtaatcct gcaccatgcc
      541 cgccgtgccg atgcggacaa aggtgtcggc ccccaccgcg atcaattcct ccaccgcgat
      601 ggccgtggag gggccgccga tgccggtgct ggtgaccgac accggcaccc cgtccagcga
      661 accagtatag gtcacatact cccggtggca ggccaccctg cgaggattgt ccagaaactt
      721 tgcgatccgc tccaccctgc ccggatcccc tggcaggatg acatagcggc ccacatcccc
      781 tttcgacagc tccacatggt attgctggcc gttgtcgttt aatgcataca taccgtcccg
      841 tctccttttt tatcccattt taaacgatgc ggctgatgat acagcgcttc agttcttctg
      901 cgtgcagctt ggtgcaggtg gtggcgttgg gcggcttgcc gctctgatgg cagtgatcca
      961 ccgcgatggc gccgtaggcc aggcccctgt tggtctccgc ccgcgtgtag gccgtgacgc
     1021 tctgctccac gagctccggc ctcagggcca ccgccatggc gaccgggtcg gcaaagtcca
     1081 gcaccggcgc gccgaaacgg cgctcgttca gctccaccag attgtggttg atgtccagac
     1141 agaatgccgc cagcggggat cccgtctgcc gcagcccctc caggtctttc tcggtgaaga
     1201 gatattcgtt gatgcacaga tcccagccca ccagcaccag ctcggtcccc gcgtcccgca
     1261 gcacgatatc cagcgcctcc gggtccatgc gcgcgttgcc ctcggccagt ggggtcgcgt
     1321 tgccgaaata ggggccggtg cccatcatca tggtgatgcg cttcagcttc gccagcgtgc
     1381 cgggggaccg caggctgatc caggccaggt tggttaccgg gcccagcgtg accatctcca
     1441 gctcgcccgg ctccgcctcg atggcgcgga tgagaaaatc taccgcgtgc tcccgctgcg
     1501 gccgcagggc cgggggcggc agtcccaggt cgcccatccc gtcccgcccg tggat
//
LOCUS       DBMDYA010000689         1550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15767_length_1550_cov_1.629400, whole genome shotgun sequence.
ACCESSION   DBMDYA010000689 DBMDYA010000000
VERSION     DBMDYA010000689.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15767_length_1550_cov_1.629400"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            157..1011
                     /locus_tag="ACZPFK_13350"
     CDS             157..1011
                     /locus_tag="ACZPFK_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1407338.1"
                     /translation="MSIYRTQEGRRQVLRLYDAQLQALGTPFEDRWVETSFARTHLVE
                     VGAKENPPLFVLHGGNATTACNLLNCRFLLPWFHIYAADLPGHPGKSDEQCLSPYGRA
                     YGKWAAEVIAALGYERLRCFGGSFGAGVLVKLMAAAPEKVEKAVLTVPAALKNALPLK
                     TLRMLGPLMRYRMTGEERWCERCFLPLSAGEQIPPWLLAAGKCSLDHVKIKAMMPCDE
                     PGRALRAYHGPVYVLAAQKDCLFPAKGVLRRAVRVWPQCRCGLLAGRGHIHSLTEEEE
                     QGILEFLR"
BASE COUNT          266 a          443 c          499 g          342 t
ORIGIN      
        1 gctatatctg ttgctgcgcg gcctgatcct acagctcacc ggctagctta cccgctgggg
       61 gaaacaaagc ggtttccctc tcttttttta gcttttttct gactattttt tattttcagt
      121 cagaaaaaac ggccgacacc gaaaggggat agggacatgt cgatttaccg cacgcaagag
      181 ggtcgccgcc aggtgctgcg cctgtatgac gcgcagctgc aggcgctggg gacgcccttt
      241 gaggacagat gggtggagac ctcgtttgca aggacgcatc tcgtggaggt gggggccaag
      301 gagaatcccc cgctttttgt actgcacggg ggcaatgcca caacggcctg taatctgctg
      361 aactgccgtt ttttattgcc ctggttccac atttacgcag ccgatctccc cgggcatccg
      421 ggcaagagtg acgagcaatg cctgtccccc tatggccgcg cctatggaaa gtgggccgct
      481 gaggtgatcg ccgccctggg ctatgagcgc ctgcgctgct tcggcggctc gtttggggcc
      541 ggggttttgg tcaagctgat ggccgcggcg cccgaaaagg tggaaaaggc cgtgttgacg
      601 gtgcccgcgg cgctcaaaaa tgcgctgccc ctcaagaccc tgcgcatgtt gggaccgctg
      661 atgcgctatc ggatgaccgg ggaagagcgg tggtgcgagc gctgtttttt gcccctctct
      721 gcgggagagc agatcccgcc ctggctgttg gcggcgggaa aatgcagcct ggaccatgta
      781 aaaatcaagg cgatgatgcc gtgtgacgag cccggcaggg ccttgcgcgc gtatcacggg
      841 cccgtctatg tcctggcggc gcaaaaggat tgccttttcc ccgccaaggg cgtgttgagg
      901 cgtgcggttc gggtgtggcc gcagtgtagg tgcgggctgc tcgcgggacg cgggcatatc
      961 cacagcctga ccgaggagga ggaacagggg attttggaat ttcttcgctg agcagcgctg
     1021 cctccccggg ggctccactg tccgccgcgc cgcttttttt agggggaatg gaaagccggt
     1081 ctcagcgagg ttttggcagc aaacggctgc cggctggccc tagcggaaaa agtccgcctg
     1141 ccgcgtcggc gtgtcggcag gccccacagg gctgcggcgc tcggttttcg tgcggtcggg
     1201 ccttttctgc aaacagctga tgccgcgtgg ttttgccctg ccggccggga cccaatatcc
     1261 ctgcgctgct gtgcgttatt ccgtccgccg cttttttata gaaatttatt tgcccgcatg
     1321 tttagaaaaa agcaaatggg ggcagactgt aggtgttgca gagctgccaa cgatgcaacg
     1381 gcttttttca tttttcttcc tcaaaaacgg cccccgcctt ctgtacagag gcgggggccg
     1441 ttttttttgc gctgcgcaag gccctcccgg gccgccccac gccgcgccgg ttttggcggg
     1501 acacaaggcg caaagacgcg cgtttccgtc tcccgcccca aaaagaggat
//
LOCUS       DBMDYA010000690         1550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15769_length_1550_cov_1.315390, whole genome shotgun sequence.
ACCESSION   DBMDYA010000690 DBMDYA010000000
VERSION     DBMDYA010000690.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15769_length_1550_cov_1.315390"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="ACZPFK_13355"
     CDS             <1..702
                     /locus_tag="ACZPFK_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR1407339.1"
                     /translation="ALGAEKGIRFSVHAPYYISMSSMEEPKRVGSVRYLLESAAAVRS
                     LGGKRIIFHSGSCGKQSREEALAKALDTMRLAIEALDEKGFADIVPCPETMGKINQLG
                     TLSEVLALCGVDKRIVPCIDFGHLNARTLGGLAAKADFAAVLDEMEQTLGDDRAKNFH
                     VHFSKIQYTQGGEKCHLTFADTEYGPNFEPFFELCYERGLTPTVICESAGTQAVDAQA
                     MKLYYESLGAPGEKK"
     gene            719..>1550
                     /locus_tag="ACZPFK_13360"
     CDS             719..>1550
                     /locus_tag="ACZPFK_13360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1407340.1"
                     /translation="MLNRCQKLALSMPEGFEAALVTTVANRFYFLDFDADDAGTLLIF
                     PDESVFLIDSRYIEIAGRTAKNARVVEEHDGLAQVRELLAARGVKRLYAEEEVSVAYA
                     GRLRAALPGVEVDLSPALSQAIRALRVIKEPEEIERMKQAQKLTDDCFTYICGKIRPG
                     AREIDLALEMEVFMRSRGAGALSFPTIFVSGPKTSMPHGEPGDKVIEQGDFVTMDYGA
                     KWGGYCADMTRTVAVGGLSAEMERVYDTVLKAQLACCRSIRAGMTGREVDAIAREIID
                     S"
BASE COUNT          281 a          484 c          526 g          259 t
ORIGIN      
        1 gcgctggggg ctgaaaaggg catccgcttt tccgtccatg cgccctatta catctcgatg
       61 tccagcatgg aggagcccaa gcgggtgggc agcgtgcgct atctgctgga gagcgcggcc
      121 gccgtgcgct cgctgggggg caagcgcatc atcttccact ccggctcctg cggcaagcag
      181 agccgggagg aggcgctggc aaaggcgctg gataccatgc gcctggccat cgaagcgctg
      241 gacgaaaagg gctttgccga cattgtgccc tgcccggaaa ccatgggcaa gatcaaccag
      301 ctgggcaccc tcagcgaggt gctggcgctc tgcggcgtgg acaagcgcat cgtgccctgc
      361 atcgacttcg ggcacctcaa cgcccgcacg ctgggcggcc ttgccgccaa ggccgatttc
      421 gccgcggtgc tggacgagat ggagcagacg ctgggggatg accgcgccaa aaattttcac
      481 gtccacttct ccaagatcca gtacacccag ggcggcgaaa aatgccatct cacctttgcc
      541 gacaccgagt acgggcccaa ctttgagccg ttttttgagc tgtgctacga gcgggggctg
      601 acccccacgg tgatctgtga gtcggcgggc acccaggccg tggacgcgca ggccatgaag
      661 ctgtattatg aatcgctcgg cgcgccgggc gaaaagaaat gaaaaggagc gatatcccat
      721 gctgaaccga tgccaaaagc tcgccctctc gatgccggag gggtttgagg ccgcccttgt
      781 gaccacggtg gccaaccgct tttacttttt ggatttcgac gcggacgacg cgggcacgct
      841 gttgatcttt cccgatgaga gcgtcttttt gatcgactcc cgctatatcg agatcgccgg
      901 ccggacggcc aagaacgccc gggtggtgga ggagcacgac ggcctggccc aggtgcgcga
      961 gctgctggcc gcgcgcggcg tcaagcgcct gtatgccgag gaggaggtca gcgtggccta
     1021 cgcgggccgc ctgcgggccg ccctgccggg cgtggaggtg gacctttccc cggccctctc
     1081 ccaggccatc cgcgcgctgc gggtcatcaa ggagccggag gagatcgagc gcatgaaaca
     1141 ggcgcagaag ctcaccgacg actgctttac ttatatttgc ggcaagatca ggcccggcgc
     1201 acgggagatc gacctggcgc tggagatgga ggtctttatg cgcagccgcg gcgcgggggc
     1261 gctgtcgttc cccaccattt ttgtcagcgg ccccaagacc agcatgcccc acggcgagcc
     1321 gggggacaag gtgatcgagc agggagattt cgtcaccatg gactacggcg ccaagtgggg
     1381 cggctactgc gccgacatga cccgcaccgt ggccgtgggc ggcctcagcg cggagatgga
     1441 gcgggtctac gacaccgtgc tgaaagcgca gctcgcctgc tgccgctcga tccgggccgg
     1501 tatgaccggc cgcgaggtgg acgccatcgc ccgtgagatc atcgacagcg
//
LOCUS       DBMDYA010000691         1549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15777_length_1549_cov_1.435083, whole genome shotgun sequence.
ACCESSION   DBMDYA010000691 DBMDYA010000000
VERSION     DBMDYA010000691.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15777_length_1549_cov_1.435083"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="ACZPFK_13365"
     CDS             <1..482
                     /locus_tag="ACZPFK_13365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1407341.1"
                     /translation="VADAEVLFIGEGPGQNEDEQGLPFVGRSGALLDTFLRTIDLDRK
                     KNIFITNTVKCRPPQNRDPLPDERAACLPWLREQFRMMKPKIVVCLGRIAAQQIIRPD
                     YSVTREHGQFTEKDGVLFMGTFHPAALLRSPGNKPIAFEDFAALRDKIREVCDHTYD"
     gene            complement(958..>1549)
                     /locus_tag="ACZPFK_13370"
     CDS             complement(958..>1549)
                     /locus_tag="ACZPFK_13370"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1407342.1"
                     /translation="RRVGKWVLHEAIVQARRLLQVFPRLSMSVNVSYKQLIDPDGGVE
                     ESLLDYVRRELEAFPLGEGRLILELTESCIIDRVDETARLMERFHELGVGVAVDDFGT
                     GYTALGILKRMPVDLVKVDRSFVQSENRSEFDEVFLKFVSRLCHQVGAEVCQEGVEDE
                     AQYRRVLSAQADYIQGFWFGRPMTVGKLIETFGKSE"
BASE COUNT          311 a          532 c          422 g          284 t
ORIGIN      
        1 gtgtggccga tgcggaggtg ctgttcatcg gcgaggggcc gggccagaac gaggacgagc
       61 agggcctgcc ctttgtgggg cgcagcggcg cgctgctgga tacctttttg cgcaccatcg
      121 acctggaccg caaaaaaaac attttcatca ccaacaccgt caaatgccgc ccgccccaaa
      181 accgcgaccc gctgcccgac gagcgcgccg cctgcctgcc ctggctgcgg gagcagttcc
      241 ggatgatgaa acccaagatc gtggtctgct tgggccgcat cgccgcacag cagatcatcc
      301 ggcccgacta ctcggtgacc cgcgagcacg gccagttcac cgaaaaggac ggggtgctgt
      361 tcatgggcac ctttcacccg gcggcgctgc tgcgcagccc gggcaacaag cccatcgcct
      421 ttgaggattt cgccgctctg cgcgataaga tccgcgaggt ctgtgaccac acttacgact
      481 gaacatgcgg caaaaaagcc gtggcgaaag ccacggcttt tttattgctc cgcaataaaa
      541 aacttatttt tatggccctg tttctcgccg ctgtcgcggc aaccgaaaca gacggccaaa
      601 aaagatagcc gcagcgcagc tatctttttt gctccgcaat aaaaaactta tttctagggc
      661 cccgtttctc tccgttgtcg cggcagccgg aacgggcgat cgaaaaaacg gcctgcgggc
      721 cgcccagccc atggccctga aatttttgag cgcccgccca cagagtgggc ggatgcgtgt
      781 aaaaagccgc tgccgcatat cgcctctcat cggcgagccg tcgcaagggg cggacgggtt
      841 gtccgttttg acctgtctgc gccggagcaa aaacagagcc cgcttctgac cagttcggga
      901 gcccttgccc cactgccgcc gctcttttga tcgctttgcc ccgtgcggcg gcaagcctta
      961 ttcgctcttt ccaaacgtct cgatcagctt gccgacggtc atcgggcggc cgaaccagaa
     1021 gccctggata tagtccgcct gtgcgctcag caccctgcgg tattgcgcct cgtcctccac
     1081 gccctcctgg cacacctccg cgcccacctg atggcagaga cgggagacga acttcaaaaa
     1141 cacctcgtca aattcacttc ggttttcgct ctgcacaaaa gagcggtcca ccttgaccag
     1201 gtccaccggc atccgcttca ggatgcccag cgccgtgtag ccggtgccga aatcgtccac
     1261 cgcaaccccc acacccagct catgaaagcg ctccatcaac cgcgccgtct cgtcgacgcg
     1321 gtcaataata caggactcgg tcagctcgag gatcagcctg ccctccccca gcggaaaagc
     1381 ctccagctcc cggcgcacat agtccagcaa gctctcctcc accccgccgt ccgggtcgat
     1441 gagctgcttg taggaaacgt tgacgctcat cgacaggcgc ggaaaaacct gcagcagccg
     1501 gcgcgcctgt acgatggcct cgtgcagcac ccatttcccc acccggcgg
//
LOCUS       DBMDYA010000692         1548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15787_length_1548_cov_1.610919, whole genome shotgun sequence.
ACCESSION   DBMDYA010000692 DBMDYA010000000
VERSION     DBMDYA010000692.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15787_length_1548_cov_1.610919"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /gene="rpoN"
                     /locus_tag="ACZPFK_13375"
     CDS             <1..1156
                     /gene="rpoN"
                     /locus_tag="ACZPFK_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR1407343.1"
                     /translation="EWSCGPGPGAQAVVGEERDPFAGVCARGPGLYDRLMLQLAASAA
                     PAQQKKDAVALAAFLDGNGYLAGLPDSLFTEGERAGRLRAALSLLQSLEPAGVGARDL
                     RECLLLQLARRGGCTALERRIVEEELGPLAQGRFEAAARRLGVPMLELRAAWRAIRTL
                     DPKPGAAFEPGEPAALALPDVEVRSEGEQLVLAVTAAERYHVAVEEPYRELQRTSPDE
                     EVRRYLGDQVARAALLCRCLQRRNETLLRVAGRIAVHQREFFVGKERRLSPLRLAQVA
                     EELGLHETTVGRAVRGKYLGCHHGVYPMRQFFTSAVGEGEAQSSSQDLKRRLALLLRS
                     EDAARPYSDAALCALLGGEEAVSRRTVAKYRAALGYPAAAARRREALCGA"
     gene            1263..>1548
                     /locus_tag="ACZPFK_13380"
     CDS             1263..>1548
                     /locus_tag="ACZPFK_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR1407344.1"
                     /translation="MESESRRMRLPEIEIDIYALDAIIQNSYDGIFITDGQAVTIMVN
                     RSYETISGLRAEQVLGRSMREIVEEGLISASGTLLALEKRRPVTLDQQFRN"
BASE COUNT          227 a          488 c          604 g          229 t
ORIGIN      
        1 tgagtggagt tgcgggccgg gccctggcgc gcaggccgtc gtgggggagg agcgggaccc
       61 ctttgcgggc gtgtgcgccc gcgggccggg gctgtacgac cgcttgatgc tgcagctcgc
      121 cgcctccgcc gcaccggcgc agcagaaaaa agacgccgtc gccctggccg cgtttctcga
      181 cgggaacggc tatctggccg gcctgccgga cagccttttc accgaggggg aacgcgccgg
      241 gcgcctgcgc gccgccctgt ctctgctgca aagcctcgag cccgccggcg tgggggcgcg
      301 ggacctgcgg gagtgtctgc tgctgcagct cgcgcgccgc ggcggctgca cggcgctgga
      361 gcgccgcata gtggaagagg agctgggccc tctggcccag gggcgttttg aagcggctgc
      421 acggcggctg ggcgtcccca tgctggagct gcgcgcggcg tggcgcgcga ttcggacgct
      481 ggaccccaag ccgggcgcgg cctttgaacc gggggagccc gcagccctgg ccctgcccga
      541 tgtggaggtc cgcagcgagg gggagcagct ggtgctggcg gtgaccgccg ccgagcgata
      601 tcatgtggcg gtggaggagc cctaccggga gctgcagcgc acctcgccgg atgaggaggt
      661 gcggcgctac ctgggcgacc aggtggcccg cgccgccctg ctgtgccgct gcctgcagcg
      721 caggaacgag acgctgctgc gggtggcggg gcgcatcgcc gtccaccagc gggagttttt
      781 tgtgggcaaa gagcgccgcc tctcgcccct gcgcctcgcc caggtggcag aggagctggg
      841 cctgcacgag accacggtgg gccgcgcggt gcgcggcaag tatctgggct gccaccacgg
      901 cgtctatccc atgcggcagt tctttacgtc ggcggtgggc gagggggagg cgcagagcag
      961 cagccaggat ctgaagagac ggctggcgct tttgctgcgg tcggaggacg cggcgcggcc
     1021 ctacagcgac gccgccctct gcgcgctgct cggcggcgag gaggccgtct cgcgccgcac
     1081 cgtggcgaag tacagggccg cgctgggata ccccgccgcg gcggcgcggc gcagagaagc
     1141 gctctgcgga gcatagagga taaaagagaa caggcagggg ccggggcttt cggggcgccg
     1201 gcccctgtgt cctgtccaca gcttggacat tggggtgggg aatggagcag acgccttttt
     1261 atatggaatc cgagtcgcga aggatgcggc tgccggagat cgagatcgac atctacgcgc
     1321 tggatgccat catccagaac tcttacgacg gcatttttat cacggacggg caggcggtca
     1381 ccatcatggt gaaccggtcc tatgagacca tctcgggcct gcgggccgag caggtgctgg
     1441 gcagaagcat gcgggagatc gtggaggagg ggctgatctc cgcctccggc accctgctgg
     1501 cgctggaaaa gaggcggccg gtgaccctgg atcagcagtt ccgcaacg
//
LOCUS       DBMDYA010000693         1547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15796_length_1547_cov_1.298064, whole genome shotgun sequence.
ACCESSION   DBMDYA010000693 DBMDYA010000000
VERSION     DBMDYA010000693.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_1547_cov_1.298064"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(524..1081)
                     /locus_tag="ACZPFK_13385"
     CDS             complement(524..1081)
                     /locus_tag="ACZPFK_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1407345.1"
                     /translation="MPPKVKVTREQIVQAALALVRESGAQALNARAVANRLGCSTQPV
                     FSNYATMEELKLDVLRAADECYQAHLAKGMRDPAYPPYKASGVAYIGFARQERELFKL
                     LFMRDRRGEKVEEDRQSVAPLLRILSANTGMSEDEAYFFHLEMWVYVHGIATMIATDY
                     LDWDEELISRMLTDAYEGMKERYKK"
BASE COUNT          297 a          510 c          377 g          363 t
ORIGIN      
        1 cggatcgtgg tggtttttcc cgccccgtta ccctcagcag cgcgaacagc tcgccgctgt
       61 ggaccgtcag gtccaggccc tccaccgccg cggtgtcccg atagtgcttg ctcagccctc
      121 tcgtcacaat agcatccatg gattttcctc ctgcatatac tgcacatact tcgcgtaaaa
      181 aacgaccgat cttgacggcc cgtttctcgc cgccgcgcgg caaccgaaac ggatggctga
      241 aaaatagcct gcggctattt tttattgctc cgcaataaaa aacaacttat ttctatggcc
      301 ccggccaggg gactggacgg atggccacaa aaaaatagcc tgcggctatt ttttattgct
      361 ccgcaataaa aaataactta tttctatggc cccggccagg ggactggccg gatggccaaa
      421 aaaatagcct gcggctattt tttattgctc cgcaataaaa aacaacttat ctctatggcc
      481 ccggccaggg gactggccgg atggccaaaa aaatagcctg cggctatttt ttgtacctct
      541 ctttcatgcc ctcataggcg tcggtcagca ttctgctgat cagctcttca tcccaatcaa
      601 gataatcggt ggcgatcatg gtggcgatgc cgtgcacata gacccacatc tcaagatgaa
      661 aaaaataggc ctcatcctcg ctcatgcccg tattcgccga gaggatgcgc agcagcggcg
      721 ccaccgactg ccggtcctcc tccacctttt ccccgcggcg gtcgcgcata aaaagcagct
      781 tgaacagctc ccgctcctgc cgggcaaagc cgatataggc gacgccgctg gccttatagg
      841 gcggataggc cgggtcccgc atgcccttgg caaggtgcgc ctgatagcac tcatccgccg
      901 cgcgcagcac gtccagcttc agctcttcca tcgtggcgta gttgctgaac accggctgtg
      961 tggagcaacc cagccggttt gccaccgcgc gggcgttcag ggcctgcgcc ccgctctccc
     1021 gcaccaaggc cagggcggcc tgcacgatct gctcccgcgt cacctttact ttcggcggca
     1081 tggcagcacc cctctcttaa aatatcaata gttatatatc aattgatatt ttactctgtt
     1141 tttctttttt gtcaagccct gccttttggc tgccagcttt ttctctttgc cgccgcccgc
     1201 ccgcggcagg tgaaaccagc ctaaagggtg ggcggaaagc ggcgctgccg gtctcccgcc
     1261 ccatcttcaa gtctcgaagc gcgcctttgg gggttgctct ccctccggcg cggcccgggc
     1321 acgccgctct tgccgggagg catacgctgc gcgctcccca gccatcccct gtctgccctc
     1381 ttcttcccgc cggtccccgc ttccatgccg ctgtctcgct ctttccctct ctccattttt
     1441 gccgccgctc cgccctcgca ccggagcgct gaaaaaacaa tcgccgtggc cgcgcttttt
     1501 tattgacttt atatcccgag atggtaaact aatactgata atccaat
//
LOCUS       DBMDYA010000694         1543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15834_length_1543_cov_1.244799, whole genome shotgun sequence.
ACCESSION   DBMDYA010000694 DBMDYA010000000
VERSION     DBMDYA010000694.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15834_length_1543_cov_1.244799"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="ACZPFK_13390"
     CDS             <1..501
                     /locus_tag="ACZPFK_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1407346.1"
                     /translation="GPAGGSPREPGLLLGGCDPWQVDLAAAALMGLPPAQVPYLAAAQ
                     RRGLCGDVFDPAQLAPGSDPIQPIPGWKLPESFSSLAFADKFPSGLRWASPLVTRLAA
                     PRPVIRRAKCVGCGKCAEICPDHTIAVRGGKAKIDPSGCIRCFCCHEMCPAKAIDVRR
                     IPFFNL"
     gene            540..1415
                     /gene="ispE"
                     /locus_tag="ACZPFK_13395"
     CDS             540..1415
                     /gene="ispE"
                     /locus_tag="ACZPFK_13395"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1407347.1"
                     /translation="MDGVTLLAPAKLNLTLDVTGVRGDGYHTMDMLMQAVSLYERVTV
                     RRAEELRVSAPGAALPLGPKNVAWKAAVAFFQQTGLLAGAEIRLEKHAPVRAGMGGGS
                     ADAAAVLMGLDLVYGARLPLGELCCIGAAVGADVPFALTGGTARVRGFGEEITPLPPL
                     RGCLFVVCMPGYGVSTPQAFAAYDRVGAGARPDNEGAERAVRAGKLQALFPCLGNALQ
                     KASAGRDTERICRLLTGAGAKAALMTGSGAATYGVFTGSAAAQRGAAALRQAGFGQVW
                     TLAPAATGPLVEEIF"
BASE COUNT          212 a          506 c          574 g          251 t
ORIGIN      
        1 ggccccgcgg ggggcagccc gcgcgagccg ggcctgctgc tgggcggctg cgacccctgg
       61 caggtggacc tggccgccgc cgcgctgatg ggcctgccgc cggcgcaggt gccgtatctt
      121 gcggcggcgc agcggcgcgg cctttgcggg gatgttttcg acccggcgca gctggcgccc
      181 ggcagcgacc cgatacagcc catcccgggc tggaagctgc ccgagagctt ttccagcctc
      241 gcctttgccg acaaattccc cagcgggctg cgctgggcca gtccgctggt gacccgcctt
      301 gccgctccgc gcccggtcat ccgccgcgcc aaatgcgtgg gctgcggcaa atgcgccgag
      361 atctgcccgg accacaccat cgcggtgaga ggcggcaagg ccaaaatcga cccctcgggc
      421 tgcatccgct gtttttgctg tcacgagatg tgcccggcaa aggcgatcga cgtgcggcgt
      481 atcccatttt ttaatcttta gatcgccgcc gggggcccgg cggcggggag gagagcgtta
      541 tggacggcgt gaccctgctg gcgcccgcaa aactgaacct gacgctggac gtcaccggcg
      601 tgcgcgggga cggctaccac accatggata tgctgatgca ggcggtgagc ctgtatgagc
      661 gggtgacggt gcggcgcgcg gaggagctgc gggtcagcgc gccgggggcc gcgctcccgc
      721 tggggccgaa gaatgtggcc tggaaagcgg cggtcgcctt ttttcagcag accggcctgc
      781 tggccggcgc ggagatccgg ctggaaaagc atgcccccgt gcgcgccggc atgggcggcg
      841 gcagcgccga cgccgcggcg gtgctgatgg gccttgacct ggtgtacggc gcgcgcctgc
      901 cgttgggcga gctgtgctgc atcggcgcgg cggtcggggc cgacgtgccc tttgccctca
      961 ccggcggcac ggcgcgggtg cgcggctttg gggaagagat cacgcccctg ccgccgctgc
     1021 ggggctgcct ctttgtggtc tgcatgccgg gttacggcgt ctccaccccc caggcctttg
     1081 cggcctatga ccgggtgggg gcgggcgctc gccccgacaa cgagggggcg gagcgcgccg
     1141 tccgggcggg gaagttgcag gccctgttcc cctgtcttgg caacgcgctg caaaaggcgt
     1201 cggcgggcag ggatacagag cgcatctgcc gcctgctgac cggcgcaggg gcaaaagcgg
     1261 ccctgatgac cggcagcggc gcggcgacct atggggtgtt cacgggcagc gctgcggcac
     1321 agcgcggcgc tgcggcgttg cggcaggcgg gctttggcca agtctggacg ctcgcccccg
     1381 ccgccaccgg ccccctggtg gaggaaattt tttgacctgc gcccgcaggt gggcaggggc
     1441 gggctcttgt gttcccctgt aaaaaaagcg accgtttccg gcgcgttgag ataacaaaac
     1501 attggtttta aaccgacggc tttgatactc cgtatcttta aaa
//
LOCUS       DBMDYA010000695         1541 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15849_length_1541_cov_1.265972, whole genome shotgun sequence.
ACCESSION   DBMDYA010000695 DBMDYA010000000
VERSION     DBMDYA010000695.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15849_length_1541_cov_1.265972"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="ACZPFK_13400"
     CDS             <1..586
                     /locus_tag="ACZPFK_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1407348.1"
                     /translation="TFNQGVDMVKKIAAVAGEDAKIGIVTAGLDVDIINQRIDGMREE
                     MKNHQGMEIVAVEDAHGDTIVAGEKATAMIQTYPEINVMVAAGASDVPGVGKAIDDLG
                     KKDDILGVAFNDDPQGLSYLKTGVYDFIIASLPYEEGYATVQAMVAVVSGDAAGMPTD
                     KVYLRSIEINADNTDTYKDSNPPSFEELVQAAAK"
     gene            647..>1541
                     /locus_tag="ACZPFK_13405"
     CDS             647..>1541
                     /locus_tag="ACZPFK_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407349.1"
                     /translation="MQEPNLAAQNKKKSALKKLLSYENIGVLAMLVVLCVGTAFVNPN
                     FLSSENVVNLLRKLSLISFISIGMAFVIIGGGIDLSADSVIGLGGIMAGVLMQAGWHP
                     LLAVLCSLLVGALFGFINGFFVTKLDIPPFIVTMGTLYIAKGCVNVICKGRPIYPLND
                     SFIALCDKLAPLGVPLSVWVMIALTAVAAFILNKTPYGRSITAAGGNEKAAVMCGIKV
                     ARMRMISYVVMGLLSAFTGVLLAARLNSAHPASGDGWGLKAIASAIIGGVSVTGGKGT
                     IMGAMIGAAIMAVLEIAMVMMK"
BASE COUNT          314 a          441 c          491 g          295 t
ORIGIN      
        1 cacctttaac cagggtgtcg acatggtgaa aaagatcgcc gcggtcgccg gcgaggatgc
       61 gaagatcggc atcgtcaccg cgggcctgga tgtggacatc atcaaccagc gcatcgacgg
      121 catgcgcgag gagatgaaga accaccaggg catggagatc gtcgcggtcg aggatgcgca
      181 cggcgatacc atcgtcgcag gcgaaaaggc gacggccatg atccagacct accccgagat
      241 caacgtgatg gtggcggcgg gcgcgtcgga cgtaccgggc gtgggcaagg cgatcgacga
      301 cctgggcaaa aaggacgaca tcctgggcgt ggctttcaac gacgatcccc aaggcctgag
      361 ctatttgaag accggcgtct atgatttcat catcgcctcg ctgccctatg aagagggcta
      421 cgccacggtg caggccatgg tggcggtcgt gagcggggat gccgcgggca tgcccaccga
      481 caaggtctat ctgcgcagca tcgagatcaa tgcagacaac acggatacct ataaagattc
      541 caacccgccc tcctttgaag aactggtcca ggcggcggca aaataataat gatgggaaaa
      601 aagccccgcc catgagggcg gggcctcctg ttgggagggt agtacgatgc aggaaccgaa
      661 ccttgcagcg caaaacaaaa agaaaagcgc actgaaaaag ctgttgtcct atgaaaacat
      721 aggcgtgctg gccatgctgg tggtgctgtg cgtgggcacg gcgtttgtga accccaattt
      781 cctctcgtct gaaaatgtgg tgaacctgct gcgcaagctg tccctgatca gttttatctc
      841 catcgggatg gcgttcgtca tcatcggggg cggcatcgac ctgtcggccg actcggtcat
      901 cggcctgggc ggcatcatgg ccggcgtgct gatgcaggcg ggatggcacc ctctgctcgc
      961 ggtcctctgc agcctgctgg tgggcgcact gttcggtttt atcaacggtt tttttgtgac
     1021 gaaactggat atcccgccct ttattgtgac catgggcacg ctgtacatcg ccaagggctg
     1081 cgtcaacgtc atctgcaagg gccgccccat ctatccgctg aacgacagct tcatcgcgct
     1141 ctgcgacaag ctggcccccc tgggggtgcc gctgtcggtg tgggtcatga tcgccctcac
     1201 cgcggtggcg gcttttatcc tcaacaaaac gccctacggg cgctcgatca ccgctgccgg
     1261 cggcaatgaa aaggcggccg tgatgtgcgg catcaaggta gcccgcatgc gcatgatctc
     1321 ctatgtggtg atgggcctcc tctccgcatt tactggtgtt ttgctggcgg cgcggctgaa
     1381 ctcggcccat cccgcatccg gcgacggctg ggggctgaaa gccatcgcct cggccatcat
     1441 cggcggggtc agcgtcacag gcggcaaggg caccatcatg ggcgccatga tcggcgcggc
     1501 catcatggcg gtgctggaga tcgcgatggt gatgatgaaa g
//
LOCUS       DBMDYA010000696         1536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15899_length_1536_cov_2.058537, whole genome shotgun sequence.
ACCESSION   DBMDYA010000696 DBMDYA010000000
VERSION     DBMDYA010000696.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15899_length_1536_cov_2.058537"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1536)
                     /locus_tag="ACZPFK_13410"
     CDS             complement(<1..>1536)
                     /locus_tag="ACZPFK_13410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1407350.1"
                     /translation="DGQEGRGVLGVAVASAAGDLQKDYPVTVPFDEATGEPAVPAGWR
                     LEAYWDGETLTAANEKILSAYQNTAYAIRPLEDGMRDLRIAVVTRSWERNGLFTSWFF
                     GNDTLYRAARSASVEHAITLIALGELLLGLPLTILPLVFRRRRMAGAAAFAKFTGWFL
                     VEFKALCAVADLWLVYQLARETLWYVGSDPATGMVVCSLVGGFLFVVPGCVLFTDLVH
                     NKGNVFRNNLFNLCRKAIHALTARSDVERGLMGRAWLAAVPGILGCAVLSFFWVTNLA
                     WYGGLGALVLLGAMAMVTAAGVLLLWLCGRTFRTVGAYTAQVHELAQGRSAQALPLKP
                     GDTFAGAEQDLMNLQEGVRRAVDQAVNVQLQAERVQLQSERMKVELIANVSHDLKTPL
                     TSVVNYADLLCEEPLEAPADRYAQVLREKAYRLKTMVQDVFDVSKAASGALPLQPELI
                     DLSRLVGQTLADMDEKIAASGLEFKVDLAPKVLVFADGAKLYRVFQNLLDNALKYAMP
                     ASRV"
BASE COUNT          285 a          530 c          481 g          240 t
ORIGIN      
        1 acccgggagg cgggcatggc gtacttgagg gcgttgtcca acaggttttg aaagacccgg
       61 tacagcttcg ccccgtcggc gaagaccagc accttgggcg ccagatcaac cttgaactcg
      121 aggcccgagg cggcgatttt ttcatccata tccgccagag tctggccgac caggcgcgag
      181 agatcgatga gctccggctg caggggcagc gccccgctgg cggccttgct cacgtcgaac
      241 acgtcctgta ccatggtttt gaggcggtag gccttttccc gcagcacctg ggcatagcgg
      301 tcggccggcg cttccagcgg ctcctcacag agcaggtccg catagttgac cacgctggtg
      361 agcggcgttt tcaggtcatg gctgacgttg gcgatcaact ccactttcat gcgctcggac
      421 tgcagctgga cccgctctgc ctgcagctgc acgttcactg cctgatccac cgcgcggcgc
      481 accccctcct gcaggttcat caggtcctgc tccgccccgg caaaggtgtc ccccggcttc
      541 agcggcaggg cctgggccga gcgcccctgc gccagctcat gcacctgggc ggtataggcg
      601 cccacggtgc ggaacgtgcg cccgcagagc cagagcagca gcacgcccgc ggcggtgacc
      661 atggccatag cccccagcag gaccagcgcg cccaggccgc cgtaccaggc gaggttcgtc
      721 acccagaaaa agctgaggac ggcgcagccc aggatgccgg gcaccgcggc cagccaggcg
      781 cggcccatca ggccccgctc cacatcgctg cgggcggtga gcgcgtggat cgccttgcgg
      841 cagaggttga acaggttgtt gcggaacaca ttccccttgt tgtggacaag gtcggtaaac
      901 agcacacagc cgggcaccac gaacagaaaa ccgcccacca gcgagcagac caccatgccc
      961 gtcgccgggt cgctgcccac ataccacaat gtctcccggg ccagctgata gacgagccag
     1021 aggtccgcca cagcgcagag agccttgaac tccaccaaaa accagccggt gaactttgcg
     1081 aaagcggctg ccccagccat ccttcggcgg cgaaatacca gcggcaaaat cgtcaacggc
     1141 agacccaaaa gcagttcgcc cagcgcgatc agcgtgatcg cgtgctccac cgaggcgctg
     1201 cgggcggcgc ggtaaagggt gtcgttgccg aaaaaccagg aggtgaacaa gccgttccgc
     1261 tcccaggacc tggtgacgac ggcgatgcgc aggtctctca tgccgtcctc cagcgggcgg
     1321 atggcatagg ccgtgttctg ataagcggag agaatttttt cgttggcggc ggtcagggtc
     1381 tcgccgtccc agtaggcctc cagcctccag cccgccggca ccgccggttc gccggtggcc
     1441 tcgtcaaaag gcacagtgac aggatagtct ttctgcaggt cgcccgcggc gctcgcaacc
     1501 gccacgccca gcacgccgcg gccctcttgg ccgtct
//
LOCUS       DBMDYA010000697         1527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_15995_length_1527_cov_1.507013, whole genome shotgun sequence.
ACCESSION   DBMDYA010000697 DBMDYA010000000
VERSION     DBMDYA010000697.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15995_length_1527_cov_1.507013"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /gene="rpsK"
                     /locus_tag="ACZPFK_13415"
     CDS             <1..263
                     /gene="rpsK"
                     /locus_tag="ACZPFK_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1407351.1"
                     /translation="WSSTGGLGFRGSRKSTPFAAQMAAETAAKAAMEHGLKSVEVFVK
                     GPGQGREAAIRALQAAGLEVNMIKDVTPIPHNGCRPPKRRRV"
     gene            283..903
                     /gene="rpsD"
                     /locus_tag="ACZPFK_13420"
     CDS             283..903
                     /gene="rpsD"
                     /locus_tag="ACZPFK_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1407352.1"
                     /translation="MARYTGAVCRQCRREGQKLFLKGERCYSDKCALTRRNFAPGQHG
                     QARKKVSEYGVQLRAKQKAKKYYGVLESQFRKYFEMAERKAGMTGENLLRILESRMDN
                     IVYRAGYAMSRAEARQLVLHRHFTVNGQIVNIPSYLVKEGDVIELRTRDCEKIKGSVE
                     KNAGRPTPVWMTADRENFKTTINRLPDRADIDFDVEEHLIVELYSK"
     gene            978..>1527
                     /locus_tag="ACZPFK_13425"
     CDS             978..>1527
                     /locus_tag="ACZPFK_13425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1407353.1"
                     /translation="MIEIERPKIDTATLSADGRYGKFVVEPLERGFGTTLGNSLRRVL
                     LSSLPGVAITSVKIDGVVHEFSTIEGVKEDVTEIVLNLKGVRAKLFSEGPKTVRIEAN
                     GECEVTAGSVQQDAEIEILNPDWHIATLDEGAKLVMELVFDKGRGYVPADRNKQNAEF
                     ATLGAIAVHSIYTPVLKVNYTVE"
BASE COUNT          346 a          449 c          451 g          281 t
ORIGIN      
        1 cctggtccag caccggcggc ctgggcttcc gtggttcgag aaaaagcacc ccgtttgctg
       61 cccagatggc ggccgagacc gctgccaagg ctgcgatgga gcatggtctg aaatccgttg
      121 aggttttcgt caagggcccc ggccaggggc gtgaagccgc gatccgcgct ctgcaggcgg
      181 ccggcctgga ggtaaatatg atcaaggacg ttacccccat tccccacaac ggatgccgcc
      241 cccccaagcg tcgccgcgtt taatttgtaa ggaggaacaa gaatggcaag atatacaggc
      301 gcagtgtgcc gtcagtgccg tcgtgaaggc cagaagctct ttttgaaggg cgagcggtgc
      361 tattccgaca agtgcgcgct tacccgcaga aactttgccc ccggccagca cggccaggcc
      421 cgcaaaaaag tttctgagta cggcgtccag ctgcgcgcga aacagaaagc gaaaaaatat
      481 tatggcgttc tcgagagcca gttccgcaaa tacttcgaga tggccgagcg caaggccggc
      541 atgaccggcg aaaatctgct caggatcctt gagagcagaa tggacaacat tgtctatcgc
      601 gccggctacg ccatgagccg cgccgaggcg cgccagctcg tgctgcaccg tcatttcacc
      661 gtcaacggcc agatcgtcaa catcccctct tatctggtga aagagggcga cgtgatcgag
      721 ctgcgcaccc gcgactgcga aaagatcaag ggcagcgtcg agaagaacgc cggccgcccc
      781 acccccgtgt ggatgaccgc cgaccgtgag aatttcaaaa ccaccatcaa tcgtctgcct
      841 gaccgcgctg atatcgactt cgacgttgaa gagcatctca tcgttgagct ttactccaag
      901 taacccgata agtagcatag ggaatgcgaa tctatggcgg gggctgtgcc tctgctttac
      961 aaggagggtt tttctgaatg attgagatcg aaaggccgaa aattgataca gccaccctca
     1021 gcgcggacgg ccgctacggc aaatttgtcg tagagcctct tgaacgcggt ttcggcacga
     1081 cgctgggcaa cagcctgcgc cgtgtgctgc tctcctccct gcccggcgtc gccatcacct
     1141 cggtgaagat cgacggtgtg gtgcatgaat tctccacgat cgagggcgtg aaagaggacg
     1201 tcaccgagat cgtcctgaac ttaaagggcg ttcgcgcaaa gcttttttcc gaaggcccca
     1261 agacggtccg tatcgaggcc aacggcgagt gtgaagtcac cgccggcagc gtccagcagg
     1321 acgccgagat cgaaatcctc aacccggact ggcacatcgc gaccctggac gagggcgcaa
     1381 agctggtgat ggagcttgtc tttgacaagg gccgcggcta tgtgcccgcc gaccgcaaca
     1441 agcagaacgc ggagtttgcc acgcttggcg cgatcgcggt gcacagcatc tacaccccgg
     1501 ttttaaaggt caactatact gtggaga
//
LOCUS       DBMDYA010000698         1524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16031_length_1524_cov_1.687280, whole genome shotgun sequence.
ACCESSION   DBMDYA010000698 DBMDYA010000000
VERSION     DBMDYA010000698.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16031_length_1524_cov_1.687280"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..142
                     /locus_tag="ACZPFK_13430"
     CDS             20..142
                     /locus_tag="ACZPFK_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407354.1"
                     /translation="MGLLLLGVVLAVRHRRRRRGRGCCGDCAACAARCAARGKK"
     gene            333..>1524
                     /locus_tag="ACZPFK_13435"
     CDS             333..>1524
                     /locus_tag="ACZPFK_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019522.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR1407355.1"
                     /translation="MKKDEERLSPRASRRLIYLLTVLVSVVFLYAGNRLAAAGGGYLP
                     SLTDGGLLRARVTAVTGRETFETEGIVAGQTVQNLTIRFTARLDGGGTVSAQQVIDGY
                     SSVQLQEVRPGDRITLYPNPDPATAAEAPYVIDDYLRVDGMLAFCAVFLVLLLIFGRM
                     KGFNTVVSLLLTCGAVFAVFLPLVLGGFNIYASAVAICIYVTVSTLLIVNGPGKKTLV
                     AVAGCSAGILFAGVLTLVMSAVMRLSGMASQEAIYLADLATRNPVDLKAVVFAAILIG
                     AMGAIMDVAMSLASALWELREKAAAPSFGMLMKSGMNIGRDMMSTMANTLVLAYIGSS
                     LATVLLLTAYSASVEGLLNREMIVTEVLQAVIGSMAILVTIPLTSLAASAVYMRGAAP
                     SRRTD"
BASE COUNT          230 a          482 c          540 g          272 t
ORIGIN      
        1 tctgatcgat tttattctca tcggcctgct gctcttgggt gtggtgctgg ccgtccgcca
       61 ccggcgcaga cgcaggggcc ggggctgttg cggcgactgc gcggcctgcg ccgcccgctg
      121 tgccgcgcgg gggaaaaaat gaactctttg cgcgccgccc tggcgacagg gcggcgcttt
      181 tggcgcccgc cgcaaaagcc ggcccggttt tttgctgcgg cggctgatct tcccacacgg
      241 ggcggacaag atcgggtata ataagggcga aacaaaacag gaccgggcgg ggatgacagg
      301 cacctgcgcc cggcccgggc ggaggcgatg ggatgaaaaa agacgaagaa cggctctccc
      361 cgcgcgcgtc gcggcggctg atctatctgc tcacggtact ggtttcggtc gtgttcctct
      421 acgcgggcaa ccggctggcg gcggcggggg gcggctatct gcccagcctc accgacgggg
      481 ggctgctgcg ggcgagggtg accgccgtca ccgggcgcga gacctttgag accgagggca
      541 tcgttgccgg gcagacggtg cagaacctga ccatccgctt caccgcccgg ctggacggcg
      601 gcggcacggt gagcgcgcag caggtgatcg acggctacag ctcggtgcag ctgcaggagg
      661 tgcgccccgg cgaccgcatc accctttatc ccaacccgga ccccgccacc gcggccgaag
      721 ccccctatgt catcgacgac tacctgaggg tggacggcat gctggccttc tgcgccgtct
      781 ttctggtgct gctgctgatc tttgggcgga tgaaaggctt caacacggtg gtgtcgctgc
      841 tgctcacctg cggcgcggtt ttcgcggtct ttctgccgct ggtgctgggc ggcttcaaca
      901 tctatgccag cgccgtggca atctgtatct atgtgacggt gagcacgctg ctgatcgtca
      961 acgggccggg caaaaagacg ctggtggcgg tggcggggtg cagcgccggc atcctgttcg
     1021 ccggggtgct gaccctggtg atgagcgcgg tcatgcggct gtccggcatg gcctcccagg
     1081 aggccatcta tctggcggat ctggccaccc gcaacccggt ggacctgaaa gccgtcgtct
     1141 tcgccgccat cctcatcggg gccatggggg cgatcatgga cgtggccatg agccttgcct
     1201 ccgccctgtg ggagctgcgc gaaaaggcgg ccgcgccctc gttcgggatg ctgatgaaga
     1261 gcggcatgaa catcggccgg gacatgatga gcacgatggc gaacacgctg gtgctggcct
     1321 atatcggctc gtcgctggcg accgtgctgc tgctgaccgc ctattcggcc tcggtcgagg
     1381 ggctgctcaa ccgggagatg atcgtcaccg aggtgctgca ggccgtcatc ggcagcatgg
     1441 cgatcctggt gaccattccg ctcaccagcc tggcggccag cgcggtgtat atgcgcggcg
     1501 ccgcgccgtc aaggcgaacc gacg
//
LOCUS       DBMDYA010000699         1517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16117_length_1517_cov_1.483757, whole genome shotgun sequence.
ACCESSION   DBMDYA010000699 DBMDYA010000000
VERSION     DBMDYA010000699.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16117_length_1517_cov_1.483757"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="ACZPFK_13440"
     CDS             complement(<1..329)
                     /locus_tag="ACZPFK_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR1407356.1"
                     /translation="MVYEFLADGFEVVEALFPVDLLRRAGIEVATVGVTGVEVASSAK
                     VTVRADLDGRGFELPPDAEMVLLPGGMPGTTNLLASPVVAAALAEAQRRDIFAAAICA
                     APWVLDA"
     gene            complement(377..1198)
                     /locus_tag="ACZPFK_13445"
     CDS             complement(377..1198)
                     /locus_tag="ACZPFK_13445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1407357.1"
                     /translation="MRTATESDRPALAALWREAFGDGPEFIDFVFDRFAGLENIWLEE
                     QDGLPAASACAVPVTMGPLRGVYLYGVNTRADLRGQGLMSRLLGQVHAEAARRGRDFA
                     VLVPEGARLFDFYARLGYETRFYHRLVEKEIRPNLWARAEFDTLTAQRLAAARQRFLE
                     PPYVAFEPQAHAAMVQNLYTDGATTVETDEGYGVFFTQGDTLVFKELAATGNLAATRI
                     LEAARQHTACDRAKLELPRYGEVFLGEGEARPYGMLKWLDGEKKLDEPSMSLMCD"
     gene            complement(1237..>1517)
                     /locus_tag="ACZPFK_13450"
     CDS             complement(1237..>1517)
                     /locus_tag="ACZPFK_13450"
                     /inference="COORDINATES: protein motif:HMM:NF021445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR1407358.1"
                     /translation="GGLIRTEGQLVAFCYGSKISDEVLGTHVEKAWHDVNGAYPIINR
                     EYARAFGGAYTYINREEDLGEEGLRKAKLSYNPAILEKKFCAVLRSGG"
BASE COUNT          246 a          555 c          464 g          252 t
ORIGIN      
        1 ttggcgtcca gcacccaggg ggcggcgcag atggcggcgg caaagatgtc ccggcgctgc
       61 gcctcggcca gggccgcggc aaccaccggg gaggccagca ggttggtggt gcccggcatg
      121 ccgcccggca gcagcaccat ctccgcgtcc ggcggcagtt caaagccgcg cccatccagg
      181 tcggcgcgca ccgtcacctt ggcgctgctg gccacctcga cgcccgtgac gcccacggtg
      241 gccacctcga tccccgccct gcgcagcagg tccaccggga acagggcctc caccacttca
      301 aacccatctg ccaaaaactc ataaaccatg gttaccgctc ctttgcacaa cgatttttcc
      361 gctttatcct atgccctcag tcgcacatca gcgacatcga gggctcgtcc agcttttttt
      421 ccccgtccag ccatttgagc atgccgtagg gccgcgcctc cccctcgccg agaaagacct
      481 cgccgtagcg gggcagctcc agcttcgccc ggtcacaggc ggtgtgctgg cgggcggcct
      541 ccagaatacg ggtggcggcc aggttgccgg tggcggccag ctctttgaaa acgagcgtat
      601 ccccctgggt gaagaacacc ccgtagccct cgtcggtctc caccgtggtg gcgccgtcgg
      661 tgtacaggtt ctgcaccatg gccgcgtgcg cctgcggctc aaacgccaca tagggcggct
      721 cgagaaagcg ctgccgcgcc gcggccagcc gctgtgccgt cagggtgtcg aactccgccc
      781 gcgcccacag gttggggcgg atctcctttt ccaccagccg gtggtaaaag cgcgtctcat
      841 agcccagccg ggcataaaaa tcaaacagcc gcgccccctc gggcaccagc accgcaaagt
      901 cgcggcctct gcgggccgcc tcggcgtgga cctgccccag caggcggctc atgagcccct
      961 ggccgcgcag gtcggcccgg gtgttgacgc cgtaaaggta gaccccgcgc agcggcccca
     1021 tcgtgaccgg caccgcacag gccgaggcgg cgggcaggcc gtcctgctcc tccaaccaga
     1081 tattctcaag gccggcgaac cggtcaaaga caaaatcgat aaattcgggc ccgtcgccga
     1141 acgcctcgcg ccagagcgcg gcgagggccg gacggtcgct ttccgttgcc gtgcgcattt
     1201 ctcccatgcg tccttccctt tgctgattgc gaggcctcag cccccgctgc gcaggaccgc
     1261 gcagaacttc ttttccagga tggccgggtt ataggagagc tttgccttgc gcaggccctc
     1321 ctcgcccagg tcctcctccc ggttgatata ggtgtacgcc ccgccgaaag cccgcgcgta
     1381 ctcgcggttg atgatcgggt aggcgccgtt gacgtcgtgc caggcctttt ccacatgggt
     1441 gcccagcacc tcgtccgaga tcttgctgcc gtagcagaaa gccaccagct gcccctcggt
     1501 gcggatcagg ccgccca
//
LOCUS       DBMDYA010000700         1516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16128_length_1516_cov_1.696113, whole genome shotgun sequence.
ACCESSION   DBMDYA010000700 DBMDYA010000000
VERSION     DBMDYA010000700.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16128_length_1516_cov_1.696113"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="ACZPFK_13455"
     CDS             complement(<1..958)
                     /locus_tag="ACZPFK_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1407359.1"
                     /translation="MDSIFKDKTLLITGGTGSFGNTVLKHFLSTDIGEIRIFSRDEKK
                     QDDMRHDYQLRYPDLAGKLKFYIGDVRNRDSIHAAMGGVDFIFHAAALKQVPSCEFFP
                     MEAVRTNIVGTDNVLAEAIEAGVKKVVCLSTDKAAYPINAMGISKAMMEKVIGARART
                     VKAQDTTICCTRYGNVMASRGSVIPLFVDQIRAGKPITVTNPSMTRFLMSLDEAVALV
                     VFAFEHAEPGDLFVQKSPASTIGDLAKAMQQLFGDTGVRIIGDRHGEKLYETLLTSEE
                     RTHATDLGGYFKVPADTRDLNYDKYFVQGTLPQAEPEAYTSHN"
BASE COUNT          261 a          480 c          489 g          286 t
ORIGIN      
        1 tgttgtggct ggtgtaggcc tccggctccg cctggggcag ggtgccctgc acgaaatatt
       61 tgtcgtagtt gaggtcgcgg gtgtccgccg ggaccttgaa atagccgccc aggtcggtgg
      121 catgggtccg ctcctcgctg gtgagcagcg tctcgtacag cttttcgccg tggcggtcgc
      181 cgatgatgcg cacgccggtg tcgccgaaca gctgctgcat ggccttggcc aggtcgccga
      241 tggtggaggc ggggctcttc tgcacaaaga ggtcgcccgg ctcggcgtgc tcaaaggcaa
      301 agaccaccag cgccaccgcc tcgtccaggc tcatcaaaaa gcgggtcatg ctggggttgg
      361 tcaccgtgat gggcttgccc gcccggatct ggtccacgaa cagcgggatg acgctgccgc
      421 ggctggccat cacgttgccg tagcgggtgc agcagatggt ggtgtcctgc gccttgacgg
      481 tgcgggcccg cgcgccgatg accttttcca tcatggcctt ggagatgccc atggcgttga
      541 tgggataggc ggccttgtcg gtggagaggc agaccacctt tttcaccccg gcctcaatgg
      601 cctcggccag cacgttgtcg gtgcccacga tgttggtgcg caccgcctcc atggggaaga
      661 actcacagct gggcacctgc ttgagagccg cggcgtgaaa gatgaaatcc accccgccca
      721 tggcggcgtg gatgctgtcc cggttgcgca cgtcgccgat gtagaacttc agcttgcccg
      781 ccaggtccgg atagcgcagc tgatagtcgt ggcgcatgtc gtcctgtttt ttttcatccc
      841 gcgaaaagat gcggatctcc ccgatgtcgg tggagagaaa atgcttgagc accgtgttgc
      901 cgaagctgcc ggtgccgccg gtgatgagaa gtgtcttgtc cttgaaaatg ctgtccatac
      961 tctatccctc tcccgcgctt tctgtgcgcg atacggcaca aaaaggcaaa aaatatgccc
     1021 gtttccaaac tgtcccggga tatgccgtcg aaatatgacc ttatccagta taccatatca
     1081 gcccccgcga gacaagaaaa cagccctcct taaactgcga attccccagc tgccgcggcg
     1141 gcgccccgtg atagtcacgg ttgccagatt tcggggccgc tatacagcgc ctgtatcccg
     1201 cccggtccag gcgcgggccg ggaaagcgcg gcggggacgg gcgccccctg gtaaaacgcc
     1261 ccaaaacggg caaaatagca caaaagggga gggaatggac ggaaaagccc ccctgcgccc
     1321 gcccgcgccg ggcttggcgc gcgcccgcgc gcccgcagcc ggccgggtgc ggcttgacgc
     1381 ggggcgccgc gttttgatac catttcaggt agttcgaaat tttcttatcc accgggcgcg
     1441 ccgccttggg ccgcgccccc gagacggcgc cctggcgcgg cccagagagc ggccccggtc
     1501 ccttgaccga agcccg
//
LOCUS       DBMDYA010000701         1512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16184_length_1512_cov_1.503898, whole genome shotgun sequence.
ACCESSION   DBMDYA010000701 DBMDYA010000000
VERSION     DBMDYA010000701.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16184_length_1512_cov_1.503898"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(378..>1512)
                     /locus_tag="ACZPFK_13460"
     CDS             complement(378..>1512)
                     /locus_tag="ACZPFK_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lacto-N-biose phosphorylase central
                     domain-containing protein"
                     /protein_id="HIR1407360.1"
                     /translation="TLRLISDIEGVRYTEGRFLPYFFPDTFHEGGDPVQEAKVNWVTA
                     RRAILRKPIDRIGYGGYLKLALDFPEFIEYVGSVCDEFRLLYENARGTTPFCFKRVAV
                     LNCWGKLRSWGNHMVHHALYYKQNYSYAGVIEALSGAPFDVQFISFEDVLRDPALLSR
                     FDVVLNVGDGDTAYTGGSVWEDPRLSAAVKKFVYNGGGFIGVGEPAGHQYQGRYLQLG
                     NLLGVEEERGFTLGRDKYNWEEHPHFITEDCVGEIDFGEGKENVYALEGATVLRQKRS
                     GVQLAVNGFGKGRGVYISGLPYSFENSRLLYRAILWAASAENRLHEWFSTNVNVEVHA
                     YVQNGKYCVVNNTYEPQTTTVYRGDGSSFVKNLAANEIVWYEI"
BASE COUNT          263 a          486 c          486 g          277 t
ORIGIN      
        1 caccggtttt tcggtgccag tgcagaaata ggggcgcccc tccaccgtga agagataggc
       61 gaagacccgg gtctgcaccc ggtccccgtc ggtggtggcg agcacgccgt tgcggttggc
      121 gtcgagcagg gatgcatagt cgaacatggt ttgacggcct cctttgtttt tgcggcgcgc
      181 cgcccgggtg aaaccggcgg gcgcgcctgt tttctccatc ctacccccgg acgaaaaaaa
      241 tgtaaagccc tgtcggcaga tggcccgggg ccggtgcgga gaaaaacaga gggaccgcgg
      301 ccgggggctt tgtcccccgg ccgcggtccc tctgcggtct gaaagcgcat ccccgcccca
      361 aagcggggga gcgccgctta aatttcgtac cagacgatct cgttggcggc cagatttttt
      421 acaaagctgc tgccgtcgcc gcggtagacc gtggtggtct gcggctcgta ggtgttgttg
      481 accacgcagt atttgccgtt ttggacatag gcgtgcacct ccacgttgac gttggtggag
      541 aaccactcgt gcagccggtt ttccgccgag gcggcccaga ggatggcgcg gtagagcagg
      601 cggctgttct caaagctgta gggcaggccg ctgatataca cgccgcgccc cttgccaaag
      661 ccgttcaccg ccagctgcac cccgctgcgc ttttggcgca gcacggtggc gccctccagg
      721 gcgtagacgt tctctttgcc ctcgccgaag tcgatctcgc ccacacagtc ctcggtgata
      781 aagtggggat gctcctccca gttgtacttg tcccggccca gcgtgaagcc gcgctcctcc
      841 tccacgccca aaagattgcc gagctgcagg tagcggccct gatactggtg cccggcgggc
      901 tcgcccacgc cgatgaagcc gccgccgttg tagacaaatt ttttgaccgc ggccgagagg
      961 cgcgggtcct cccagacgct gccgccggta taggcggtgt cgccgtcgcc cacgttgagc
     1021 accacgtcga agcgggacag cagcgccggg tcgcgcagca cgtcctcaaa gctgatgaac
     1081 tggacgtcaa agggcgcgcc ggacagcgcc tcgatcaccc ccgcatagct gtagttctgc
     1141 ttgtagtaaa gcgcgtgatg caccatgtgg ttgccccagc tgcgcagctt gccccagcag
     1201 ttgagcaccg ccacccgctt gaaacagaac ggggtggtgc cgcgggcgtt ttcgtacagc
     1261 aggcggaact cgtcgcagac gctgcccaca tactcgatga actcgggaaa atccagcgcc
     1321 agcttgagat agccgccgta gccgatgcgg tcgatgggct tgcgcaggat ggcgcgccgc
     1381 gcagtgaccc agttgacctt ggcctcctgc accgggtcgc cgccctcgtg gaacgtgtcg
     1441 gggaagaaat agggcaaaaa gcgcccctcg gtgtagcgca ccccctcgat gtcgctgatc
     1501 agccgcagcg tg
//
LOCUS       DBMDYA010000702         1511 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16199_length_1511_cov_1.375177, whole genome shotgun sequence.
ACCESSION   DBMDYA010000702 DBMDYA010000000
VERSION     DBMDYA010000702.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16199_length_1511_cov_1.375177"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="ACZPFK_13465"
     CDS             complement(<1..664)
                     /locus_tag="ACZPFK_13465"
                     /inference="COORDINATES: protein motif:HMM:NF016359.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HIR1407361.1"
                     /translation="MTLEVDITERQLAFVKAQAFEVLFGGAAGGGKSYGQLIDALLYA
                     LRYPGSKQLILRRTYPDLERSLILEHLKFYPRGVYRYSSTGHRGSFVNGSTIEFGYCD
                     AERDVYRYQGAEYDVIRFDELTHFTESMYTYLISRVRGANGYPKAVKSSTNPGGVGHA
                     WVKARFIDLGAPDTVHALPTGTRLFLPSRLMDNRFLMEKDPGYLTRLENLAEADRRAL
                     RDG"
     gene            complement(661..>1511)
                     /locus_tag="ACZPFK_13470"
     CDS             complement(661..>1511)
                     /locus_tag="ACZPFK_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIR1407362.1"
                     /translation="APGQTGTEEWKAVFPARNEDGTLWTAPLRRAETSGWKVRLPAQD
                     EDSLPRAARPGGGGDRPGDAALLSGRKSVSPQAARSPRGKKDVSAPRTGAAAPAAKAS
                     AATKSTSAATKDVSQVSAVDFPGAERKAALREAMLGSAEENASQASAVDFPGTERAAT
                     PRASASDSAKKSASGCGQSSDGFRAARPAAPESVPGPSGTAEPEPCGKAAGAARVLEE
                     LTDIALGRRDYPDVDRAGNPVVRPVPMTQRLKALELLGKRYRLFSDGPAAPEEEELEV
                     RLRVIR"
BASE COUNT          197 a          549 c          500 g          265 t
ORIGIN      
        1 ccccgtcgcg cagcgcccgc cggtcggcct cggccaggtt ttccagccgg gtcagataac
       61 cggggtcttt ttccatcaaa aagcggttgt ccatcagccg ggagggcaaa aacagccgcg
      121 tgccggtggg cagggcgtgc accgtgtccg gcgcgccgag gtcgatgaag cgggctttca
      181 cccaggcgtg gcccacgccg ccggggttgg tgctgctttt taccgccttg gggtagccgt
      241 tggccccgcg cacccggctg atgaggtagg tgtacatgct ctcggtgaag tgggtcagct
      301 cgtcaaagcg gatcacgtcg tactcggcgc cctggtagcg gtagacgtcc cgctcggcgt
      361 cgcagtagcc gaactcgatg gtggagccgt tgacgaagct gccccggtgg ccggtgctgc
      421 tgtagcggta gaccccccgg gggtaaaatt tcaaatgctc caggatcagc gaccgctcca
      481 ggtcgggata ggtgcgtcgc aggataagct gctttgagcc cggatagcgc agcgcgtaga
      541 gcagcgcgtc gatgagctgc ccgtagcttt tgccgccccc cgccgcgccc ccgaacagca
      601 cctcgaacgc ctgcgccttt acaaaggcca gctgccgctc tgtgatgtcg acctccagcg
      661 tcaccggatt accctcagcc ggacctccag ctcctcttcc tccggggcgg ccgggccgtc
      721 gctgaacagc cggtagcgct tgcccagcag ctccagcgct ttcaggcgct gggtcatggg
      781 caccggccgc accaccgggt tgcccgcccg gtccacgtcc gggtagtccc tgcggcccag
      841 ggcgatgtcg gtcagctcct ccagcacccg cgccgcaccg gccgccttgc cgcacggctc
      901 cggctccgcc gtccccgacg gcccggggac gctttcaggc gctgcgggcc tggccgcgcg
      961 gaaaccgtcg gacgattggc cgcagcccga ggcgcttttt ttcgccgagt cggatgcgga
     1021 cgcgcgaggc gtagctgctc tctccgtgcc ggggaaatcc accgccgacg cctgggaggc
     1081 gttttcctct gccgagccga gcatggcttc cctgagcgcg gcttttctct ccgcgccggg
     1141 aaaatccacc gccgacacct gggaaacatc tttcgtcgcc gcggaggtgc ttttggtcgc
     1201 tgcggacgct ttggccgccg gggcggccgc ccctgtgcgc ggcgcggaca cgtctttttt
     1261 gccccgcggg gagcgcgccg cctgcggcga aacacttttg cgccccgaga ggagcgccgc
     1321 gtcccccggg cgatccccgc ccccaccggg ccgcgccgcc cgcggcaggc tgtcttcgtc
     1381 ctgcgcgggg aggcgcactt tccacccgct ggtctcggcc cgcctcaggg gcgccgtcca
     1441 cagcgtgccg tcctcattcc ttgctggaaa aacagctttc cactcctctg tcccggtctg
     1501 tcccggggcc g
//
LOCUS       DBMDYA010000703         1509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16220_length_1509_cov_1.745028, whole genome shotgun sequence.
ACCESSION   DBMDYA010000703 DBMDYA010000000
VERSION     DBMDYA010000703.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16220_length_1509_cov_1.745028"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="ACZPFK_13475"
     CDS             complement(<1..856)
                     /locus_tag="ACZPFK_13475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1407363.1"
                     /translation="MKHVFIVNPKSGKADASQYFVPALIERIAPLKLDYAVEITAHPG
                     HATEIAKQYAMAREPVRFYACGGDGTLNEVLTGAFRYPEAEVACVPLGSGNDFLKNFG
                     TAEDFLDLEDNISGWAVPIDLMQVGEDQISAAICSVGLDAAVAYGIPKYRRIPFCGGK
                     MAYNISIVENICKPMGHRMTVSVDGKNYTGNFLLAAVANGTTYGGGFRAAPRARMDDG
                     LLDVLLVNKVSRFKVASVIAKYQAGRHFSGEEIIPELRQVITFVRGRQVSIIPEKEIV
                     TNIDGECDL"
     gene            1081..>1509
                     /locus_tag="ACZPFK_13480"
     CDS             1081..>1509
                     /locus_tag="ACZPFK_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR1407364.1"
                     /translation="MEILHIILTSLGSILALFLLTKLIGNRQMSQMSLFDYINGITIG
                     SIAAEMATSLEDDFLKPLTAMIVYALVAVCISLVTCRSMALRKFFNGKPLVLLEHGKL
                     YKKNLLSAKMDINEFLTQCRSAGYFDLTQLEAAVLETNGQV"
BASE COUNT          299 a          473 c          422 g          315 t
ORIGIN      
        1 gcaggtcgca ctcgccgtcg atgttggtga cgatctcctt ttccgggatg atcgagacct
       61 ggcgcccgcg cacaaaggtg atgacctgcc gcagctcggg gatgatctcc tcgccgctga
      121 aatgccggcc cgcctgatat ttcgcgatca cactggccac cttgaagcgg ctgactttat
      181 tcaccagcag cacatccagc aggccgtcgt ccatgcgcgc ccggggcgcg gcgcgaaagc
      241 cgcccccata agtggtcccg ttcgccaccg cggccagcaa aaaattgccg gtgtagtttt
      301 tgccgtccac cgacaccgtc atgcggtggc ccatgggctt gcagatattt tccacaatcg
      361 agatattata ggccattttg ccgccgcaaa aggggatgcg gcggtacttg gggatgccgt
      421 aggcgaccgc cgcgtccagg cccaccgagc agatcgcggc cgagatctgg tcctccccca
      481 cctgcatcag gtcgatgggc accgcccagc cggagatgtt gtcctccaga tcgagaaaat
      541 cctccgccgt gccgaaattt tttaaaaagt cattgcccga ccccagcggc acacaggcga
      601 cctccgcctc cggataacgg aacgcccccg tcagcacctc gttgagggtg ccgtcgccgc
      661 cgcaggcgta aaagcgtacc ggctcgcgcg ccatggcgta ttgctttgca atttcggtgg
      721 cgtggccggg gtgcgcggtg atctcgacgg cataatccag cttgagaggg gcgatgcgct
      781 cgatcagggc cggcacaaaa tactggctgg cgtccgcctt gcctgatttg gggttgacga
      841 taaaaacgtg tttcatctca atgcacctcg ctggggaagt ttaatcctct ctcatcatac
      901 ctcatttcac tgtgagatac aacaaattcc tgtgtgaaag caatcctgtt ttgtggattt
      961 cgcacagccg tcctcgcgtg tcaacgcgcc gctgccgcag agccgcgcat aacccgcttt
     1021 cggcttggca gactaatgcc gtgagggcgc gcgccccatc accataaagg aggtcctccc
     1081 gtggaaattt tgcacatcat tctgaccagc ctgggctcta ttttggccct cttcctgctc
     1141 accaaattga tcggcaaccg gcagatgagc cagatgagcc tgttcgacta catcaacggc
     1201 atcaccatcg gctcaatcgc cgccgaaatg gcgaccagcc tggaggatga ttttctcaag
     1261 cccctgaccg cgatgatcgt ctatgccctg gtggccgtct gcatctcgct tgtgacctgc
     1321 cgctccatgg cgctgcgaaa gtttttcaac ggcaagccgc tggttctgct ggagcacggc
     1381 aagctgtaca aaaaaaacct gctgtcggcc aaaatggaca tcaacgagtt cttgacccag
     1441 tgccggagcg cgggttattt tgacctgacc cagctggagg ccgccgtgct ggagaccaac
     1501 ggccaggtg
//
LOCUS       DBMDYA010000704         1509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16227_length_1509_cov_1.257102, whole genome shotgun sequence.
ACCESSION   DBMDYA010000704 DBMDYA010000000
VERSION     DBMDYA010000704.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16227_length_1509_cov_1.257102"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="ACZPFK_13485"
     CDS             <1..873
                     /locus_tag="ACZPFK_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative NPN-dependent ornithine cyclodeaminase"
                     /protein_id="HIR1407365.1"
                     /translation="KVRIVEFRNLKRGDLVVTGRSEDGSEGIYLWTGGFAEEGDARDA
                     FAFRQGRSRETGYTRDYNELVELLRHERQYGNILWVMGPACAFDAGARKAFAALVRAG
                     YVNGLMAGNALATHDMEASYLGTALGSDIYTQQPRENGHYNHLDTINKVRECGSIPAF
                     VKAGHVTDGIIYECVQNSVPFVLVGSVRDDGPLPEVYGSVYEGQDAMRRLVRSATTVI
                     CMATTLHTIATGNMTPSYRLVDGTVRPLYFYSIDVSEFSVNKLRDRGSLSVRTIVTNV
                     QDFVGKLAGALGAE"
     gene            1272..>1509
                     /locus_tag="ACZPFK_13490"
     CDS             1272..>1509
                     /locus_tag="ACZPFK_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1407366.1"
                     /translation="MVDHASITFFEQLYEDTYASAVLFVTRRCGDPAQIPDILQETYA
                     EVYRVLLRKGRAYFKNPQALVLHIAKTRLHRYYSW"
BASE COUNT          286 a          469 c          447 g          307 t
ORIGIN      
        1 aaagtccgca tcgtggaatt ccgcaacctg aaacggggcg acctggtggt gaccggccgc
       61 agcgaggacg gcagcgaggg catctatctg tggaccggcg gctttgcgga ggagggcgac
      121 gcccgggatg cgttcgcttt tcgccagggg cgctcgcggg agaccggcta tacccgtgac
      181 tataacgagc tggtggagct gctgcgccac gaaaggcaat acggcaacat cctttgggtg
      241 atgggcccgg cctgcgcttt cgacgcgggc gcgcgcaagg cctttgccgc gctggtgcgg
      301 gcgggctatg tgaacggcct gatggcgggc aacgccctcg ccacccatga tatggaagcc
      361 agctatctgg gcaccgccct gggcagcgat atctacaccc aacagccccg ggaaaacggc
      421 cactacaatc acctggacac catcaacaag gtgcgggaat gcggctctat ccccgctttt
      481 gtcaaagcgg ggcatgtcac cgacggcatc atctatgaat gcgtgcagaa cagcgtgccc
      541 tttgtgctgg tgggctcggt gcgggacgac gggccgctgc ccgaggtcta cggcagcgtt
      601 tacgagggcc aggacgccat gcgccggctg gtgcgcagcg ccaccaccgt catctgcatg
      661 gccaccaccc tgcacaccat cgccaccggc aacatgaccc ccagctatcg cctggtcgac
      721 ggcacggtgc gcccgctcta tttttattcc atcgacgtct ccgagttttc ggtgaacaag
      781 ctgcgggatc ggggcagcct gtcggtgcgc accatcgtga ccaatgtgca ggactttgtg
      841 ggcaagcttg ccggggcgct gggcgccgag tgaggcccgc cgctctgtgt gagagcggct
      901 ctcccgcctt ttttgagctc atcccccctt gaacactttt taccgggcaa ccggcgcggg
      961 agaaaacata taaaagctgc aaaaacagcg gcaaagcgca aaaaacgcac ctgaaaaggc
     1021 tcttatgcct cttttcaggt gcgttttatc attttttcag gcagaggaca agcgatcctc
     1081 cattccctct cgcgccgcgt ttgtccaccg gcgctctgcg cctttgagcg gtttttccgc
     1141 acagcgtttt gttttttcat ggctcggcat atcggctgtc ggccacttcg gcgtcctttt
     1201 ttgagctgca gaaaaacgct gcaacttttt tgccccacgg cggcacgata gaagtgaagg
     1261 cggtgaaata aatggtcgac cacgcatcca tcaccttttt tgagcagctg tacgaagaca
     1321 cctatgcctc tgccgttctc tttgtcaccc gccgctgcgg ggacccggcg cagatccccg
     1381 atattctgca ggagacctat gccgaggtct atcgggtgct gctgcgcaag gggcgcgcct
     1441 actttaaaaa cccgcaggcc ctggtgctgc acattgccaa aacccgtctg caccgctact
     1501 actcctgga
//
LOCUS       DBMDYA010000705         1502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16320_length_1502_cov_1.693790, whole genome shotgun sequence.
ACCESSION   DBMDYA010000705 DBMDYA010000000
VERSION     DBMDYA010000705.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16320_length_1502_cov_1.693790"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            21..>1502
                     /gene="mutL"
                     /locus_tag="ACZPFK_13495"
     CDS             21..>1502
                     /gene="mutL"
                     /locus_tag="ACZPFK_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1407367.1"
                     /translation="MAVIHVLDKHTAELIAAGEVVERPASVVKELVENSIDAGSTAIS
                     VEIARGGVGSIEVSDNGSGIEAEYISTAFVRHATSKIAREEDLAAIGTLGFRGEALAS
                     IAAVAKVDLLTRTEADEYACLYRQAGGEELSAEAAARPVGTTITVRDLFYNVPARMKF
                     LKKDASEGNYVSDVVLRQALAHPEISFRFVKDGREQFHTPGDGDAMSAIYAVLSREFA
                     KNLMPVSYRQGALAVQGYVTAPIACRGSRSMQFFYINGRQVEDRTLMAALETAYKGMA
                     MQGRFPGCVLNVTMPLERVDVNVHPAKTEVRFANAGEIFDLMYKAVKSAVARPDAPQK
                     TLDLSRASGTAPSEPAGAERGAERIPAPRAKPLLPSAAQLEMPGAVPPILHAPEAAQR
                     ANEADAGAGELLRQTAAVAYETRRTARLLDIEKTPQEDFAGGGDLRAAAPSGPTAAVL
                     RTENAAGGKAGAAPAAGQEVFPEATESEPQSAAKPLEYVGEAFR"
BASE COUNT          274 a          473 c          541 g          214 t
ORIGIN      
        1 ggaaagggcg gtgctgcgca ttggcagtta ttcatgtgtt ggacaagcat accgcggaac
       61 tcatcgcggc aggcgaggtg gtggagcgcc ccgcctcggt ggtcaaagag ctggtcgaaa
      121 actcgatcga cgcgggcagc accgccatct cggtggagat cgcccgcggc ggcgtgggca
      181 gcatcgaggt cagcgacaac ggcagcggca tcgaggcgga gtatatctcc acggcctttg
      241 tgcgccacgc caccagcaaa atcgcgcggg aggaagatct tgcggccatc ggcacgctgg
      301 gctttcgcgg cgaggcgctg gccagcatcg cggcggtagc aaaggtcgat ctgctcaccc
      361 gcaccgaagc ggacgaatac gcctgtctct accggcaggc gggcggggag gagctctccg
      421 ccgaggcggc ggcccgcccg gtgggcacca ccatcacggt gcgggacctg ttttacaacg
      481 tcccggcgcg gatgaagttt ctcaaaaaag acgccagcga gggcaattat gtcagtgatg
      541 tggtgctgcg gcaggctttg gcgcaccccg agatctcgtt ccgcttcgtc aaggacggca
      601 gagagcagtt ccacaccccg ggcgacgggg atgccatgag cgcgatctac gccgtgctct
      661 cgcgggagtt tgccaaaaac ctgatgccgg tctcttaccg gcagggcgcg ctggcggtgc
      721 agggctatgt caccgccccc atcgcctgcc gcggcagccg ctcgatgcag ttcttttata
      781 tcaacggccg ccaggtggaa gaccgcaccc tgatggcggc tctggagacg gcctacaagg
      841 gcatggcgat gcaggggcgg tttccgggct gtgtgctgaa cgtcaccatg ccgctggagc
      901 gggtggacgt caacgtgcac ccggcaaaga ccgaggtgcg ctttgccaac gccggcgaga
      961 tcttcgacct gatgtacaag gcggtcaaga gcgccgtcgc gcggccggat gcgccgcaga
     1021 agacgctgga cctctcccgc gcttcgggga cggccccctc cgagcccgcg ggggccgaaa
     1081 ggggggcgga gcgcataccc gcacccagag caaagccgct gctgccgtcg gccgcacagc
     1141 tggagatgcc cggcgccgtg ccgcctattc tgcacgcccc cgaagcggcg cagagagcga
     1201 acgaggcgga tgccggggcg ggagagctgc tgcgccagac ggcggccgtg gcctatgaga
     1261 cccgccgcac cgcgcggctg ctggatattg aaaagacacc gcaggaggac tttgcgggcg
     1321 ggggagacct gcgcgccgca gcgccgtcgg ggccgacggc ggctgtcctg cgcacagaga
     1381 acgccgccgg gggaaaagcc ggggcggcgc ccgccgcggg ccaggaggtt ttccctgaag
     1441 cgacagaaag cgagccacaa agcgccgcaa agccgttaga atatgtgggg gaggcctttc
     1501 gc
//
LOCUS       DBMDYA010000706         1490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16484_length_1490_cov_1.149748, whole genome shotgun sequence.
ACCESSION   DBMDYA010000706 DBMDYA010000000
VERSION     DBMDYA010000706.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16484_length_1490_cov_1.149748"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="dapF"
                     /locus_tag="ACZPFK_13500"
     CDS             complement(<1..500)
                     /gene="dapF"
                     /locus_tag="ACZPFK_13500"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1407368.1"
                     /translation="MLEFTKMQGAGNDFIVFDGMARTLPEYGLLARAVCSRRFGVGAD
                     GILVARASDDADLAMVYYNADGSRAAMCGNGMRCFAKFARENGLTAKNVFTVETLDGV
                     KRVRVTVSGEGVVTRVTVEMGRPVFLPYELPVLLPGERVADRPLEADGRRWRVTCVRV
                     GVPHTV"
     gene            634..1230
                     /locus_tag="ACZPFK_13505"
     CDS             634..1230
                     /locus_tag="ACZPFK_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1407369.1"
                     /translation="MDTREQILQTALRQFSRRGYHAVSIRDICGEVGIKESSLYYHFT
                     NKRALLEALLERFAQKAAHFTRLLDEGTAQPAGMDDAAFLQVGEGYVQGYLLDPEILC
                     FFGMLRIERENDPALAALYREWYFERPLQFQTSMFERLAQAGYFPAGQAQRLALLYLA
                     PILFFYERELPLDGEDARARLLQAVRRHLEDFLKEHRK"
     gene            1227..>1490
                     /locus_tag="ACZPFK_13510"
     CDS             1227..>1490
                     /locus_tag="ACZPFK_13510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1407370.1"
                     /translation="MKTLQNFVITGGARGIGFHLALGLLQNGGRVAVLDLDVSPLQRQ
                     GAQLPGLLALPCDVRDAGQVAACVDRAAEEFGGIDCAVHNACLC"
BASE COUNT          269 a          501 c          463 g          257 t
ORIGIN      
        1 atcacggtgt ggggcacccc cacccgcacg caggtgacgc gccaccgccg cccatcggcc
       61 tccagcggcc ggtcggccac ccgctcgccg ggcagcagca ccggtaattc atagggcaaa
      121 aagaccgggc ggcccatctc caccgtgacc cgggtcacca ccccctcgcc gctcaccgtc
      181 acccgcaccc gcttgacccc gtccagcgtc tccacggtaa agacgttttt tgccgtaagg
      241 ccgttttcac gggcaaattt ggcaaagcag cgcatgccgt tgccgcacat ggccgcccgg
      301 ctgccgtcgg cattgtaata gaccatggcc aggtccgcat cgtcgctggc cctggccacc
      361 aagatgccgt cggcccccac cccgaagcgc cggctgcaga ccgcccgcgc caacagccca
      421 tactccggca gggtgcgcgc catgccgtca aagacgataa aatcgttgcc cgcgccctgc
      481 atcttggtaa actccagcat attgacccct ccttgcgatc tcgccgcgct tcatctctac
      541 gcaaaaaaag cgaaaaaaat gtgtgcttga ctttctaacg atcgttaggt aaaattaatc
      601 taacgatcgt tagattggag gaaaagcgaa atcgtggaca ccagggaaca gatcttgcag
      661 accgcactgc ggcagttctc ccgccgcggt taccacgccg tctcgatccg cgacatctgc
      721 ggcgaggtgg gcatcaagga gagctcgctc tactatcatt tcaccaacaa gcgcgcgctg
      781 ctggaggcgc tgctggagcg ctttgcccaa aaggcggcgc acttcacccg cctgctggac
      841 gaggggacgg cgcagccggc aggcatggac gacgcggcgt ttttgcaggt gggggagggc
      901 tatgtgcagg gctacctgct ggacccggag atcctctgct ttttcgggat gctgcgcatc
      961 gagcgggaaa acgaccccgc gctggccgcg ctgtatcggg aatggtattt tgagcggccg
     1021 ctccagtttc agaccagcat gttcgagcgc ctggcccagg cggggtactt cccggcgggg
     1081 caggcacagc ggctggcgct gctctatctg gcgcccatcc tcttctttta tgagcgggag
     1141 ctcccgctgg acggcgagga cgcccgggcc cggctgctgc aagcggtccg ccgccacctg
     1201 gaggattttt taaaggagca cagaaaatga aaacactgca aaatttcgtg atcaccggcg
     1261 gcgcgcgggg catcggcttt cacctcgccc tcgggctgct gcaaaacggc ggccgggtgg
     1321 cggtgctgga cctggacgtc tcgccgctgc agcggcaggg cgcgcagctc cccggcttgc
     1381 tcgccctgcc gtgcgacgtg cgggacgccg ggcaggtggc cgcctgcgtg gacagggccg
     1441 ccgaggagtt cggcggcatc gactgcgcgg tgcacaacgc ctgcctgtgc
//
LOCUS       DBMDYA010000707         1489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16492_length_1489_cov_1.613833, whole genome shotgun sequence.
ACCESSION   DBMDYA010000707 DBMDYA010000000
VERSION     DBMDYA010000707.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16492_length_1489_cov_1.613833"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="ACZPFK_13515"
     CDS             complement(<1..1074)
                     /locus_tag="ACZPFK_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1407371.1"
                     /translation="MKRPLALSGFAWLATQLACTLFGWELPLWAPLALAAVFVLCAGL
                     LLRFPRSVRRLARRRTALVLFSAALALAWQVGWRALIVSPAQALAGRTAQVEAVVLRT
                     YEVREGERAQTDLQVTCVDGKKLRPAVTVKVSSLPLRQPGDRVRGELRFSLPGSYDRG
                     RGFLLRASAVSELEVTGRSNAPRYLFARLQQTLSLRLRSCFTPEIGGVAAAVCLGDRT
                     ALPQRVQDNYRRAGVSHLLVVSGLHLSLAAGAVGLLLPRRRKLLRGAVLVSAILAFMA
                     LTGFSPSVCRAGVMALFVAVGELLSESADPLTSLGAAALGLTAVNPAASADVGLLLSF
                     SATLGVLAAGGWARHRRLLRRERE"
     gene            complement(1075..>1489)
                     /locus_tag="ACZPFK_13520"
     CDS             complement(1075..>1489)
                     /locus_tag="ACZPFK_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR1407372.1"
                     /translation="EALLKKVPEFDVIVTAEAKGIPLAYEMARQSGKNYIPARKGPKL
                     YMKEPIIVEDKSITTEAQQRLVIDKKDLISMDGKRILIVDDVISTGGSLHALETLVGY
                     SKGNVVARAAVLAEGDAAQRTDIIFLEELPLFFHD"
BASE COUNT          290 a          523 c          467 g          209 t
ORIGIN      
        1 ctctctttcg cgccgcagca gacgcctgtg ccgcgcccag ccgcccgccg ccagcacacc
       61 cagggtggcg ctgaaagaga gcagcagccc cacatccgcc gatgcggcgg gattgaccgc
      121 tgtgagcccc agcgccgccg cccccaggga ggtgagaggg tccgccgatt cgctgagcag
      181 ctcccccacc gcgacgaaaa gggccatcac cccggcacgg cacaccgagg gggaaaagcc
      241 ggtgagcgcc ataaaggcca gaatcgcgga aaccagcaca gcgccgcgca aaagcttgcg
      301 cctgcgcggc agcagcagcc ccacggcccc cgccgccaag gagaggtgca gcccgctgac
      361 caccagcaga tgggagaccc ccgcgcggcg atagttgtcc tgcacccgct gcggcagagc
      421 ggtgcggtcc cccaggcaga ccgccgccgc caccccgccg atctcaggcg tgaagcagct
      481 gcgcagccgc aggctgagcg tctgctgcag ccgtgcgaag agatagcgcg gcgcgtttga
      541 ccgcccggtc acctccagct ctgacacagc ggaggcgcgc agcaaaaaac cgcggccgcg
      601 gtcatagctg cccggcagcg aaaaacgcag ttcccccctc acccggtccc ccggctgacg
      661 cagcggcaag ctggatacct tgacagtcac cgcaggcctc agtttttttc catcgacaca
      721 ggtgacctgc agatcggtct gggcgcgctc cccctcccgc acctcatagg tgcgcagcac
      781 caccgcctct acctgtgccg tccgcccggc aagtgcctgg gccggagaga cgatcagggc
      841 gcgccagccc acctgccatg ccagggccag cgctgccgaa aaaaggacga gagcggtccg
      901 ccgccgcgcc agcctgcgca cggatcgggg aaagcgcagc aacaggcccg cgcagagaac
      961 aaaaactgcc gccagggcca aaggggccca gagcggcagt tcccacccaa aaagggtaca
     1021 ggcaagctgg gttgccagcc aggcaaaccc agacagtgcc agcggccgtt tcactcagtc
     1081 gtgaaagaac aggggcagct cttccaggaa gatgatgtcg gtgcgctgtg cggcgtcgcc
     1141 ctcggcgagg acggcggcac gcgccacgac attgcccttg ctgtagccaa ccagcgtctc
     1201 caaagcgtgc agcgagccgc cggtgctgat gacgtcgtcg acgatgagaa tgcgcttgcc
     1261 gtccatgctg atcaggtctt ttttatcaat gaccagcctc tgctgcgcct cggtggtgat
     1321 ggacttatcc tcgacgatga tgggctcttt catgtaaagc ttggggcctt tgcgggcagg
     1381 gatataattt ttgccgctct ggcgggccat ttcataggcc agggggatgc ccttggcctc
     1441 cgcagtcacg atgacgtcga actccggcac ctttttgagc agcgcctcg
//
LOCUS       DBMDYA010000708         1473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16665_length_1473_cov_2.043732, whole genome shotgun sequence.
ACCESSION   DBMDYA010000708 DBMDYA010000000
VERSION     DBMDYA010000708.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16665_length_1473_cov_2.043732"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(96..1145)
                     /locus_tag="ACZPFK_13525"
     CDS             complement(96..1145)
                     /locus_tag="ACZPFK_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR1407373.1"
                     /translation="MKGKMKVAVMNGIGRMGFTERDIPQPKPDEVLVKLEYVGICGSD
                     LHYYESGRIGDYIVEPPFVLGHEPGGTVVEVGSAVTHLKVGDRVALEPGKTCGHCEFC
                     RTGRYNLCPDVVFFATPPVDGVFQEYVAHEAGLCFKLPDNVSTLEGALIEPLAVGFHA
                     ANQGGAEAGQVAVVSGAGCIGLVSMMALKAKGVSRVYVIDIMQKRLDKALELGASGVI
                     NAAETDPVEAVKELTGGRGCDLFIETAGTEATTRQAIQIAKKGSTIVLVGYSKSGEMT
                     LPMSLALDKELTFKTVFRYRHIYPMAIEAVAEGKVDLKGVVTDIFDFDDIQNAMDKSV
                     SNKADIVKAVVRIAK"
BASE COUNT          299 a          433 c          387 g          354 t
ORIGIN      
        1 aaatgctact acctttttca tgttctttct cctttttggt tttttgcatc tatgacgtcc
       61 cggtactggg tttcccgggc aatgcgacga aaaagttatt ttgcgatccg caccacggcc
      121 ttgacgatgt ccgccttgtt ggacacgctc ttatccatgg cgttttggat gtcgtcgaag
      181 tcgaagatgt cggtgacgac gcctttcagg tcgactttcc cctcggccac cgcctcaatc
      241 gccatggggt agatgtggcg atagcggaac acggtcttga aagtgagctc cttgtccagc
      301 gccaggctca tgggcagcgt catctcgccg gattttgagt agccgaccag cacgatggtg
      361 gaaccctttt tggcgatctg gatggcctgc cgggtggtgg cctcggtgcc cgcggtctcg
      421 ataaagaggt cgcagccccg gccgccggtg agctctttca ccgcctcgac cgggtcggtc
      481 tcagcggcgt tgatcacgcc cgaggccccc agctccagcg ctttatccaa acgcttttgc
      541 atgatgtcga tcacatacac ccgcgagacg cccttggcct tgagggccat catcgacacc
      601 aggccgatac agcccgcgcc gctgaccacg gcaacctggc ccgcctcggc gccgccctgg
      661 ttggcggcgt ggaagccgac cgccaggggt tcgatgagcg cgccctccag cgtggagaca
      721 ttgtccggca gcttgaagca gagccccgcc tcatgggcca catactcctg gaatacgccg
      781 tccaccggcg gggtggcgaa gaacaccacg tccggacaga ggttgtagcg gccggtgcgg
      841 caaaactcgc agtggccgca ggtcttgccc ggctccagcg ccacccggtc gccgactttc
      901 aggtgggtga cggcgctgcc cacctccacc acggtgccgc ccggctcatg ccccagcacg
      961 aaaggcggct ccacgatgta atccccgatg cgcccgctct cataatagtg caggtcgctg
     1021 ccgcagatgc ccacatattc cagcttgacc agcacctcgt ccggtttcgg ctgcgggata
     1081 tcccgttcgg taaagcccat cctgccgatg ccgttcatca cggcgacttt cattttgcct
     1141 ttcaacccag tcagacctcc tgttcgtaga gagaagaata cgaaacgtaa acgattgtat
     1201 tttcgcgctg cagagcgttt ctctctcttt cactttttgc tcagagcgtg attttcataa
     1261 aagtaatcgt ttattcacac gcttttgccg tgcggcagaa aatgttttat agaaaaagag
     1321 agaaatgaaa aagaaacagt taagttttaa atcgtttgcg taaccgtttt tctaagctga
     1381 tttaagcata aactcttttc gactcactgt caattgacat aattttataa agttaatgag
     1441 taaaatttgt agatagttta cacgtaactg tta
//
LOCUS       DBMDYA010000709         1471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16698_length_1471_cov_1.600730, whole genome shotgun sequence.
ACCESSION   DBMDYA010000709 DBMDYA010000000
VERSION     DBMDYA010000709.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16698_length_1471_cov_1.600730"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="ACZPFK_13530"
     CDS             <1..690
                     /locus_tag="ACZPFK_13530"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0097216 - guanosine tetraphosphate binding
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit assembly factor BipA"
                     /protein_id="HIR1407374.1"
                     /translation="YEFQVSPPKVLLHTDEAGNKLEPYELVVIDVPTAYQGSVIEKLS
                     GRKGELVEMHPQGDTRMRLEFRMPSRGLFGYRSEFLSATRGEGIMNTIFDGYGEYKGD
                     LPTRTTGSLISFETGTAVTYGLYNAQERGTLFIGAGEEVYEGMVVGSNPKGDDMAVNV
                     CKTKHLTNTRASGSDDALRLIPPRRFSLEEALEFLNTDELLEVTPKSYRIRKQILKND
                     LRMKATKGKKA"
     gene            complement(883..1119)
                     /locus_tag="ACZPFK_13535"
     CDS             complement(883..1119)
                     /locus_tag="ACZPFK_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407375.1"
                     /translation="MREIDAGAVKITLEKAIKASGLSKNKVAQLAGMQRTQLNNYCKG
                     NIQRVDLDILARLCTALECSVSDILEFVPPDVRK"
     gene            complement(1216..>1471)
                     /locus_tag="ACZPFK_13540"
     CDS             complement(1216..>1471)
                     /locus_tag="ACZPFK_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407376.1"
                     /translation="RAAHRRRLLRLFGSIKRKKLISTKKHLREFGSSRRCFCCFPYFC
                     FSDGKKGIECCRENSFMGWLFAITAKLCQKEIVFHGSSLC"
BASE COUNT          355 a          425 c          368 g          323 t
ORIGIN      
        1 tacgagttcc aggtctcccc ccccaaggtg ctgctgcaca ccgacgaggc gggcaacaag
       61 ctggagccct atgagctggt ggtcatcgac gtgcccaccg cgtatcaggg ctcggtcatc
      121 gaaaagctgt ccggccgcaa gggcgagctg gtggagatgc acccgcaggg cgacacccgc
      181 atgcgcctcg agttccgcat gccgtcccgc ggcctgttcg gctaccgcag cgagttcctc
      241 tccgccaccc gcggcgaggg catcatgaac accatcttcg acggctacgg cgagtacaag
      301 ggcgacctgc ccacccgcac caccggctcg ctgatcagct tcgagaccgg caccgccgtc
      361 acctacggcc tgtacaacgc gcaggagcgc ggcaccctgt tcatcggggc gggcgaggag
      421 gtctacgagg gcatggtggt cggctcgaac cccaagggcg acgacatggc ggtcaacgtc
      481 tgcaagacca agcacctgac caacacccgc gcctcgggca gcgacgacgc gctgcggctg
      541 atcccgccgc gccgcttcag cctggaggag gcgctggaat ttctcaacac cgacgagctg
      601 ttggaggtca cccccaagag ctaccgcatc cgcaagcaga ttttgaaaaa cgacctgcgg
      661 atgaaagcca ccaagggaaa aaaggcgtaa cacaaaaaga acggctgcgg gaagcagccg
      721 ttctttttta tctcttttca tgcagaggat ccatcgtcct gttctctttt gcctgttgga
      781 ggctcgctca cagatataaa accgtttgcg cccgcttttt cctctcttcg ccctcgtcct
      841 ggcgtcaaaa atgatggccg aaaaaatgtc cgcggctatt ttctattttc gtacgtcggg
      901 cgggacaaat tccagaatat cagaaacaga acactccagg gcagtgcaca aacgcgctaa
      961 aatatccaaa tcaactcttt ggatatttcc tttacaataa ttgtttaatt gcgttctctg
     1021 catccccgca agctgcgcaa ctttattttt gctcagacca gaggctttga tggctttttc
     1081 cagagtgatt ttaactgcgc cggcatctat ttctctcaca tcctcacctc tttgttccat
     1141 tgtagggtgc gtgcatcccg aaaaatagat gcatttatga tattaataga tatatacagc
     1201 tgtatatatc tattgtcagc ataaggagga accatgaaaa acaatctctt tctggcaaag
     1261 ctttgcggta atagcgaaca gccagcccat aaaagaattt tccctgcagc actctatacc
     1321 tttcttgcca tctgaaaaac aaaaatatgg aaaacaacaa aagcatctgc gtgaagaacc
     1381 gaactcacgc agatgctttt ttgtggaaat gagttttttg cgctttatac tcccgaatag
     1441 gcggagaagc cgccgtcgat gggcagcacg g
//
LOCUS       DBMDYA010000710         1462 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16811_length_1462_cov_1.384276, whole genome shotgun sequence.
ACCESSION   DBMDYA010000710 DBMDYA010000000
VERSION     DBMDYA010000710.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16811_length_1462_cov_1.384276"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="ACZPFK_13545"
     CDS             complement(<1..1131)
                     /locus_tag="ACZPFK_13545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR1407377.1"
                     /translation="MSRRIEDTDRVAELAQRFAYDESLLYDALVGSTDEYVYVCNPRT
                     NTIRYPARMIAEFDLPGEVIHDPVPKWKHIIHPQDWQRFYEANTEPFDYSRVNYHIVE
                     YRAKNRRGQWVWLQCRGIRLCDASGEPALFAGYISDMGRRNKVDQQTGLYNQVEFERQ
                     TRALAADPAGTPFGLLVLGMDNFRQVNERYDRQFGDEVLRAVTQKMLSTLPGNAGLYR
                     LSGDQYGLLAHHCGVGEIAALYEKIQRQLDHQQELSGKKYYCTVSAGCALCPQDALGY
                     EELYRRAECAYDYAKKHGKNRLCFFSQEVLDDSEKSTELILRLQESVEHGCSGFTVLY
                     QPQVLAKTGELRGVEALLRWDCPAFPGIGPSEFVPVLESCGLI"
BASE COUNT          264 a          463 c          419 g          316 t
ORIGIN      
        1 gatcaggccg cagctctcca gcaccgggac aaattccgac gggccgatgc cggggaaagc
       61 cgggcagtcc cagcgcagca gcgcctccac gccgcgcagc tcccctgttt ttgccagcac
      121 ctgcggctgg tagagcaccg taaagccgga gcagccgtgc tccacgctct cctgcaggcg
      181 caaaatgagc tcggtgctct tctcgctgtc gtccagcacc tcctgcgaaa aaaagcacag
      241 tcggtttttt ccgtgtttct tggcgtagtc atacgcgcac tcggcgcgcc ggtacagctc
      301 ttcatagccc agggcgtcct gcgggcagag cgcgcagcct gccgacacgg tgcagtagta
      361 ttttttgccg ctcagctcct gctgatggtc cagctggcgc tggatctttt catagagagc
      421 ggcgatctcg cccacaccgc agtgatgcgc caaaagaccg tactggtcgc ccgacaggcg
      481 gtaaaggccg gcattgccgg gcagggtgga gagcatcttc tgggtgacgg cgcgcagcac
      541 ctcgtcgccg aactgccggt cataccgctc gttcacctgg cggaaattgt ccatccccag
      601 caccagcagc ccgaacggcg tgccggctgg gtcggctgca agggcccgcg tttgccgttc
      661 aaactccacc tggttgtaca ggccggtctg ctgatccacc ttgttgcgcc gccccatatc
      721 cgagatatag cctgcgaaca gggcgggctc acccgaagcg tcgcagagcc ggatgccccg
      781 gcattgcagc cagacccact gcccgcgccg gttcttggcg cggtattcca caatgtggta
      841 attgacgcgg ctgtagtcaa agggttcggt gttggcctca taaaagcgct gccagtcctg
      901 cgggtggatg atatgcttcc actttggcac cgggtcgtgg atcacctcgc cgggcaagtc
      961 gaactcggcg atcatgcggg cggggtagcg tatggtgttg gtgcgcgggt tgcacacata
     1021 aacgtactcg tcggtgctgc ccaccagcgc gtcatacagg aggctctcgt cataggcaaa
     1081 ccgctgtgca agctccgcca cccggtcagt gtcttctatc cgacgcgaca tttctccatc
     1141 gcccctttcg ccctgccggg cctgctggga tcggctttct cttccctgtt acaagatata
     1201 ggatagcaca cctttttcac ccatgcaatt gatttcacaa tttccggaca caaaagtcgc
     1261 ttatttttat ttattatcag ttttattgcg gcataaaact caattatccg catgataagg
     1321 caaacagacc ggcgcaaacg cgccggtctg tttgcctttt ttcacttgtg gggggcagga
     1381 gctgccgcct gtttattcca ggtcctcccg gcccgcgttg gcgtcgttcg gccagacgag
     1441 ctccgccttg aacagatcgc cg
//
LOCUS       DBMDYA010000711         1460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16835_length_1460_cov_1.499632, whole genome shotgun sequence.
ACCESSION   DBMDYA010000711 DBMDYA010000000
VERSION     DBMDYA010000711.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16835_length_1460_cov_1.499632"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(178..>1460)
                     /locus_tag="ACZPFK_13550"
     CDS             complement(178..>1460)
                     /locus_tag="ACZPFK_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1407378.1"
                     /translation="FIEEKRPARAVVVGGGFIGLEMAENLTRAGLSVTLVERMGQVLA
                     PLDYDMASLLHAHLRDKGVTLRLGASVAGFEECGDGVRTLLEGGEALTADLVLLAIGV
                     APDTHLAEKAGLALGGRKSIAVDEGMRTSDPDVYAVGDAVQVKNILTGVPVLLPLAGP
                     ANKQARIAADNICGRPSRFSGVQGTSVLKLFDLTAASTGLNEAAARAAGITFDKVVTF
                     SANHASYYPGSSNMTVKVLFAPDTGRLLGAQIVGAEGADKRIDVLATAIRGGLTGADL
                     TELELAYAPPYSSAKDPVNMAGYVIENLLTGTVRQYHWDQVDALPRDGSVTLLDTRTD
                     AEYAAGHIEGAVHIPLDSLRRRLSELDPAKPLYVNCQSGLRSYIACRILSQNGFECRN
                     LSGGYRFYEIVQRQKALDTAPAHPCGLPVDQKKA"
BASE COUNT          225 a          515 c          487 g          233 t
ORIGIN      
        1 cggcgcagcg ccggaaaaat ttccacaggc cgcccgcctg cgcgggcggt cttcggggcg
       61 aaattgcaag ccccctgcgg ctgcggaatg aatttgacga cgctccgcgc gcaaatcccc
      121 ctcgcgggag acaatgccct cccgccaagg cgcgcccctg ccccacagct tttatactca
      181 ggcttttttc tgatccaccg gcagcccgca ggggtgtgca ggcgcggtgt ccagagcttt
      241 ctgccgctgc acgatctcat aaaagcggta gccgcccgag aggttgcggc actcaaagcc
      301 gttctggctg aggatgcggc aggcgatata gctgcgcagg ccgctctggc agttgacata
      361 gagcggcttt gccgggtcca gctcggacag acgccgtcgc aggctgtcca gtgggatatg
      421 cacggcgccc tcgatgtgcc cggccgcata ttcggcgtcg gtgcgggtgt ccagcagggt
      481 gacgctgccg tcccggggca gggcgtccac ctgatcccag tgatactggc gcaccgtgcc
      541 ggtgagcagg ttttcgatga cgtagcccgc catgttcacc gggtccttgg cggaggagta
      601 gggcggcgcg taggccagct ccagctcggt gaggtccgcg ccggtcaggc cgccgcggat
      661 ggccgtcgcc agcacgtcga tgcgcttgtc ggccccctct gcgcccacga tctgggcgcc
      721 gagcagacgc ccggtgtccg gcgcaaaaag caccttgacc gtcatgttgg aggagcccgg
      781 gtaataggag gcgtgattcg ccgaaaaggt gaccaccttg tcgaaagtga tccccgccgc
      841 cctggccgcc gcctcattga ggccggtgga ggctgcggtc aggtcgaaca gcttgagcac
      901 gctggtgccc tgcacgccgg aaaagcggct gggccgcccg cagatgttgt ccgccgcgat
      961 gcgggcctgc ttgttggccg ggcccgccag gggcagcagc accggcacgc cggtgaggat
     1021 gttcttcacc tgcaccgcgt cccccacggc gtagacgtcc gggtcggagg tgcgcatgcc
     1081 ctcgtccacc gcaatggact ttcggccgcc cagcgccagc ccggccttct ccgccagatg
     1141 ggtgtcgggc gcgacgccga tggccagcag caccagatcc gccgtcagcg cctcgccgcc
     1201 ctccagcagg gtgcgcacgc cgtcgccgca ctcctcaaag ccggccaccg aggcgcccag
     1261 ccgcagcgtc acgcccttgt cccgcaggtg ggcgtgcagc aggctggcca tgtcgtagtc
     1321 cagcggcgcc agcacctggc ccatccgctc caccagcgtg accgacaggc cggcgcgggt
     1381 caggttttcg gccatctcca ggccgatgaa gccgccgccc accaccacgg cccgggcggg
     1441 ccgcttttct tcaataaaac
//
LOCUS       DBMDYA010000712         1456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16873_length_1456_cov_1.533579, whole genome shotgun sequence.
ACCESSION   DBMDYA010000712 DBMDYA010000000
VERSION     DBMDYA010000712.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16873_length_1456_cov_1.533579"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            33..>1456
                     /locus_tag="ACZPFK_13555"
     CDS             33..>1456
                     /locus_tag="ACZPFK_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1407379.1"
                     /translation="MKKKIIKSMAAVLSLGLLLCTLIASLVFEARFTQRAKQDMQRLV
                     ASAALEAELLGGRDEAAARRISDAAGGLRVTFVDADGTVSGDSAADPAAMENHADREE
                     IETARTAKYGVSVRGSATTGRNMLYVATRLSDGSFLRLADTYPSALAGFVSFLPALLV
                     AAAVAFAITLVLADRFAKSITGPIEELSDSLKLVRSGGAALQPDRYQYDELQDMASDI
                     NQLSAEVDENLASLEKERARIDYVLDNMSEGLILLDAEETVLTVNRAACGFLGCDKSV
                     TGKNIIYATRHIAFINAVDGAVQGAKTARVEMPLPSGALVEAVVSPVAGRQGQQASAE
                     AAIAVLSDVTIRKNAVKMRQEFFSNASHELKTPITSIRGFAELLCGPAELPEEKRLEF
                     ARRILKEAATMQNLISDIIMISRLEAGDITFEREVLDLAEVVRDCCADARPLAQQQGI
                     SLTCTAEKAVLSASRKEMQELAGNL"
BASE COUNT          255 a          467 c          516 g          218 t
ORIGIN      
        1 cccgccgcgg ccggatgggg agagtggacg gtatgaaaaa aaagatcatc aaaagcatgg
       61 ccgcggtgct cagcctgggc ctgctgctct gcaccctgat cgcctcgctg gtgtttgagg
      121 cgcggttcac ccagcgcgcc aagcaggata tgcagcggct ggtcgcctcg gcggccctgg
      181 aggcggagct gctgggcggg cgggacgagg ccgccgcccg gcggatctcc gacgcggcgg
      241 gcggcctgcg ggtcaccttt gtggacgccg acggcacggt cagcggcgac tcggccgccg
      301 acccggcggc gatggaaaac cacgccgacc gggaggagat cgagaccgcc cgcacggcga
      361 agtacggcgt gtcggtgcgc ggctcggcca ccacggggcg caacatgctc tatgtcgcca
      421 cccgcctgtc cgacggcagc tttttgaggc tggccgacac ctatccctcg gcgctggccg
      481 gttttgtcag ctttttgccc gccctgctgg tggcggcggc cgtagcgttc gccatcacgc
      541 tggtgctggc cgaccggttt gccaaaagca tcaccggccc catcgaagag ctgtccgaca
      601 gcctcaagct ggtgcgcagc ggcggggccg ccctgcagcc ggaccgctat cagtacgacg
      661 agctgcagga catggcctcg gacatcaacc agctgtcggc ggaggtggac gaaaacctgg
      721 ccagcctgga aaaagagcgg gcccgcatcg actatgtgct ggacaatatg tccgagggcc
      781 tgatcctgct ggatgcggag gagacggtgc tcaccgtcaa ccgggcggcc tgcggctttc
      841 tgggctgcga caagtcggta acgggcaaaa acatcatcta cgccacccgc cacatcgcct
      901 ttatcaacgc cgtggacggg gcggtgcagg gggcgaagac ggcgcgggtg gagatgccgc
      961 tgcccagcgg cgccctggtg gaggcggtcg tcagcccggt ggcggggcgg caggggcagc
     1021 aggcgtcggc cgaggcggcc atcgccgtgc tctcggacgt caccatccgc aaaaacgcgg
     1081 tcaagatgcg gcaggagttc ttctccaacg ccagccatga gctcaagacc cccatcacct
     1141 cgatccgggg ctttgccgag ctgctgtgcg gcccggccga gctgccggag gaaaagcgcc
     1201 tcgagtttgc ccggcgcatc ctgaaagagg cggctaccat gcagaacctg atcagcgaca
     1261 tcatcatgat cagccgtctg gaggccgggg acatcacctt tgagcgggag gtactggacc
     1321 tggcggaggt ggtgcgggac tgctgtgccg acgccaggcc gttggcgcag cagcagggga
     1381 tatcgctcac ctgcacggcg gaaaaagcgg tcctatccgc ctcccgcaag gagatgcagg
     1441 agctggcggg caacct
//
LOCUS       DBMDYA010000713         1453 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_16905_length_1453_cov_1.509615, whole genome shotgun sequence.
ACCESSION   DBMDYA010000713 DBMDYA010000000
VERSION     DBMDYA010000713.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16905_length_1453_cov_1.509615"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1011..>1453)
                     /locus_tag="ACZPFK_13560"
     CDS             complement(1011..>1453)
                     /locus_tag="ACZPFK_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1407380.1"
                     /translation="DLLDETLEESTNLPFTGGKRMVDVDKVRDIIDDVRLNMPAEIKQ
                     AKAIVNDRAEIVAGARREAEGIIKKAEDRARALVDQQEIVRQAQQKAAEMLSAAQQQS
                     REMRTTITDYCENMLRQTEEILARSAAEVKNVRGALRQKAKNGG"
BASE COUNT          266 a          449 c          409 g          329 t
ORIGIN      
        1 catgaggatg cccacgatga ccaggccgta gagcagcggg ctgctcgcaa tttctttaaa
       61 tcccataacg attcctccct ataatttccc gtcgcagacg gggcatgaca tgatcgggcg
      121 gcgggcgggg cgcgcaggcg cttttcgtcc agccttgcag ggcggcccgg gcagcgcagg
      181 cgcgcgtttt ccgttctctc cagcgcgctt taccctgcgg cgcggtaaaa agcctcgctt
      241 tctccccgcc gggcgtcaaa ttgcaaccgc aacagtgtga tctttttgtt ttttgtattc
      301 agtcatctgt tctttcacga aagactgccg cagccaattt gtaacataga atatcaaatc
      361 ttaacaaaaa attcaagaat tattcgcaaa aaatattttt atttttggag gtttattgac
      421 ctcatccaaa cttttctgcg attttaccga caagatgacg ttttggtaaa aacctttgga
      481 cgaaatgcca ctatcatttt tgagtatgac ctgctacagc aaggcgcttt gaaagcggtt
      541 tcgcaaggtg ttttttgcgg ccagagcggt gcccgaagac ggcgcagata ttttttcttt
      601 cgcctattcc gccgcggcga aacagcgcgc ataaaagcaa gccatgcccc ccccggggtc
      661 aaagccaggt ctcaaggggc gcagtgcgcg cctgccccgg cgagaggagg cggcgcggac
      721 gcagagacga acagcatcgc agccgaagcc ccccacttgc cggcgtgacc gcctccccta
      781 aaaagggaac cctgcggccg ccgcaggccg gagcatgggg atacgggcga ttttcggcaa
      841 tcaaaacagc ccccgcttaa agcgggggct gttttgattg ccatcggtcc tcccgccgca
      901 cagcggcgcg ggaggccggg gccgttaagc tctgtttttc ttttgctgct gttccgggcg
      961 gcggtcaaaa agcgcgatcc gccagggccg gggccccggt ccggtgcagg tcagccgccg
     1021 tttttcgcct tttgccgcag cgcgccgcgc acatttttga cttcagctgc gctgcgggcg
     1081 aggatctcct ccgtctggcg cagcatattc tcgcagtaat cggtgatggt ggtgcgcatc
     1141 tcgcgcgact gctgctgggc ggcggacagc atctcggccg ccttctgctg ggcctggcgc
     1201 acgatctcct gctggtccac cagggcacgg gcgcgatcct ccgccttttt gatgatcccc
     1261 tccgcctcgc gcctcgcccc ggccacgatc tcggcccggt cgttcacgat ggccttggcc
     1321 tgcttgatct cagcgggcat attgagccgc acgtcgtcga tgatgtcccg caccttatcc
     1381 acgtctacca tccgtttacc gcccgtaaag ggcaggttgg tcgattcctc cagcgtctcg
     1441 tccagcaggt cca
//
LOCUS       DBMDYA010000714         1445 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17025_length_1445_cov_1.078125, whole genome shotgun sequence.
ACCESSION   DBMDYA010000714 DBMDYA010000000
VERSION     DBMDYA010000714.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17025_length_1445_cov_1.078125"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            392..>1445
                     /locus_tag="ACZPFK_13565"
     CDS             392..>1445
                     /locus_tag="ACZPFK_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1407381.1"
                     /translation="MKKLSRACGAAAALVLCACLCAPAAAAAESGQLRFPDDGKLKIA
                     VFADTQTTQNVSQNLIDGLEAVLEAEQPDLVIYLGDQVEGTHPFLHLGDNEAHVKRVI
                     DQVLEPVVSRGIPFAVVFGNHDAQDAGVPKEVQMAYYRSFPGCLAVDEGDALPGCGTY
                     SLLYYSADGSRPALNLYLIDSLEYDAGGGYGCVSKEQIAWCRTVGETLARTGGGRPVP
                     ALAFQHIIVPEVYNTFTQVRDKGVEGAFEGRGVGAGRWYTADAPAVTGELLEAPCPPD
                     YSNGQFAAWLAAGDVRAAFFAHDHLNSFTARLNGIDLVACPGATFTSYNGDAARGVRI
                     IEIDEDEIGEGRYETRV"
BASE COUNT          210 a          473 c          526 g          236 t
ORIGIN      
        1 ggttttttgg ggtgcatctg ggcatgggcc tggtgggcat ctggtgggcc atggccgcgg
       61 acgagtgcct gcgcgcggtg atctttctct tccgcttcgg ctccggcgtg tggaagaaga
      121 agctggcggg cctcggcggc cgtgaagagg cgacctgacc ccgcgttgcg ccaccttttt
      181 tcagcaaagc cgcggggcgc ggcgcgcccc ggcgaaccgg gcgggccccg cccctttcgc
      241 cgcggtcccg ccgcgccgcc ccctttgccg taaaggacct gattcgcccc gggacccgcc
      301 ggggccccgg ctcgtcctat tttgggggtg cccgccgccg ccgtttgtgt tatactcagg
      361 acaagaagca atttgagaac ggagggatcc tgtgaaaaag ctttcgcggg cctgcggggc
      421 ggccgcggcc cttgtgctct gcgcctgcct gtgcgcgccg gcggcggccg cggcggagag
      481 cgggcagctg cgcttccccg acgacggcaa gctgaagatc gccgtctttg cggacacgca
      541 gacgacccag aacgtgtcgc aaaacctcat cgacgggctg gaggcggtgc tggaggccga
      601 gcagcccgat cttgtgatct atctgggcga ccaggtggag ggcacccacc cctttttgca
      661 cctgggggac aatgaggccc acgtgaaacg ggtcatcgac caggtgctgg agccggtggt
      721 cagccggggc atcccctttg ccgtggtgtt cggcaaccac gacgcgcagg acgccggggt
      781 gcccaaagag gtgcagatgg cctattaccg cagctttccc ggctgcctgg cggtggacga
      841 gggggacgcc ctgcccggct gcggcaccta cagcctgctc tattacagcg ccgacggcag
      901 ccgcccggcc ctgaacctgt acctgatcga ctcgctggaa tacgacgcgg gcggcggcta
      961 cggctgtgtg agcaaggagc agatcgcctg gtgccgcacc gtgggcgaga cgctggcgcg
     1021 cacgggcggc ggccggccgg tacccgcgct ggcttttcag cacatcatcg tgcccgaggt
     1081 ctacaacacc tttacccagg tgcgggacaa gggggtggaa ggcgcgtttg agggccgggg
     1141 cgtgggcgcc gggcgctggt ataccgcgga cgcccccgcc gtcaccggcg agctgctgga
     1201 ggccccctgc ccgcccgatt actcaaacgg tcagttcgcg gcctggctgg cggccgggga
     1261 tgtgcgggcg gccttttttg cccacgatca cctgaactcg ttcaccgccc ggctgaacgg
     1321 catcgacctg gtggcctgcc cgggcgcgac gttcaccagc tataacgggg acgcggcccg
     1381 gggggtgcgc atcatcgaga tcgacgagga cgagatcggc gaggggcgct acgagacgcg
     1441 ggtgc
//
LOCUS       DBMDYA010000715         1443 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17044_length_1443_cov_1.201192, whole genome shotgun sequence.
ACCESSION   DBMDYA010000715 DBMDYA010000000
VERSION     DBMDYA010000715.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17044_length_1443_cov_1.201192"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..1008)
                     /locus_tag="ACZPFK_13570"
     CDS             complement(73..1008)
                     /locus_tag="ACZPFK_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR1407382.1"
                     /translation="MEKAQREKAKDSNLITREYFDDLLVEMRHIGAAAPDTGMTLYGR
                     RFSTPVMMAALSHLKGQGGEGDGMVQMAQGALLAGAVNWAGMGANEQFAAIAATGAPT
                     VRIIKPYADEELVRQRIRQAEELGALAVGMDLDHAFNAAGRCDTVLGYPMRPRTLAEI
                     EGYCRGTSLPFIVKGVLSAADAKKCLEAGVGGIVVSHHHGVLPSIVPPLMVLPEIVEA
                     VRGRMPVFVDCGFMNGADVFKALALGATAVSIGRPVMPGIAEAGAEGAAAVINAVTAE
                     LAGMMARTCSPDLGSIDRSLLWKRDGAHLSPLSFA"
     gene            complement(1046..>1443)
                     /locus_tag="ACZPFK_13575"
     CDS             complement(1046..>1443)
                     /locus_tag="ACZPFK_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1407383.1"
                     /translation="LEKVGLPFDERLVVNLPDVRSKVAISAAMQLINSDDRPDAFFTA
                     SDVFACAVIRAAYLAGFRVPGDLLVVGFDNIDITSTSVPSITTVNQPKLQLGFSACEI
                     LIEKIQNPSVPPKKVILETEMIIRESTLV"
BASE COUNT          217 a          499 c          483 g          244 t
ORIGIN      
        1 tccgcgctcc tgcgcggagc ggcggcccca ggcgctcttc tcctggctcc cctttacagc
       61 cgccgcccct tttcaggcaa aggacagcgg ggacaggtgg gcgccgtccc ttttccagag
      121 cagggagcgg tcgatgctgc ccaggtccgg gctgcaggtg cgcgccatca tgccggccag
      181 ctcggcggtg accgcgttga tgaccgccgc cgccccctcg gcgcccgcct cggcgatgcc
      241 gggcatcacc gggcggccga tgctgaccgc cgtggcgccc agggccaggg ccttgaagac
      301 gtcggcgccg ttcatgaagc cgcagtccac aaagaccggc atccgccccc ggacggcctc
      361 gacgatctcg ggcagcacca tcagcggcgg cacgatggag ggcagcacgc cgtggtgatg
      421 ggagaccacg atgccgccca ccccggcctc caggcacttt ttggcgtccg ccgcgctgag
      481 cacccccttg acgataaagg gcagactggt gccccggcag tagccctcga tctcggccag
      541 ggtgcgcggg cgcatgggat accccagcac agtgtcgcac cggcccgcgg cgttgaaggc
      601 gtggtccagg tccatgccca ccgccagcgc gcccagctcc tccgcctggc ggatgcgctg
      661 gcgcaccagc tcctcgtcgg cgtagggctt gatgatgcgc acggtgggcg cgccggtggc
      721 ggcgatggcg gcgaactgct cgttggcgcc cataccggcc cagttgacgg ccccggccag
      781 cagcgcgccc tgcgccatct gcaccatgcc gtccccctcg ccgccctgcc ctttcaggtg
      841 ggagagggcg gccatcatga ccggggtgga aaagcggcgg ccgtacaggg tcatgccggt
      901 gtcgggcgcc gcggcgccga tgtgccgcat ctccaccagc agatcgtcga aatactcgcg
      961 ggtgatcagg ttggaatcct ttgccttttc gcgctgtgcc ttttccattt ttttgctctc
     1021 ctttttatcg cttgcccgca ttctctcaga ccagggtgga ctcgcgtatg atcatctcgg
     1081 tctccagaat cacctttttc gggggcaccg aggggttctg gatcttttcg atcaggatct
     1141 cgcaggccga gaaccccagc tgcagcttgg gctggttgac ggtggtgatg gaggggaccg
     1201 aggtggaggt gatgtcgatg ttgtcaaagc ccaccaccag caggtcgccc gggacgcgga
     1261 acccggccag ataggccgcg cggatcaccg cgcaggcaaa gacgtccgag gcggtgaaaa
     1321 aggcgtccgg gcggtcgtcc gagttgatga gctgcatggc cgccgagatc gccaccttgg
     1381 agcgcacgtc cggcaggttg accaccagcc gctcgtcaaa gggcaggccc accttttcca
     1441 gcc
//
LOCUS       DBMDYA010000716         1442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17060_length_1442_cov_1.374348, whole genome shotgun sequence.
ACCESSION   DBMDYA010000716 DBMDYA010000000
VERSION     DBMDYA010000716.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17060_length_1442_cov_1.374348"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            996..>1442
                     /locus_tag="ACZPFK_13580"
     CDS             996..>1442
                     /locus_tag="ACZPFK_13580"
                     /inference="COORDINATES: protein motif:HMM:NF022812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HIR1407384.1"
                     /translation="MKKRICAFWLAAAMALGLFGCGETAQGQGPGPTGGPAADSGRGA
                     FLAAQQDQYDPALVPAVGRYTVAEDFSNVINYSDWDRAFSDEAKQMLLQNGFVVVGDG
                     MEEFFSLYENNRYSLTPNFVTTDAMMHTYHLFFSRLLKNVEREHFCA"
BASE COUNT          304 a          395 c          412 g          331 t
ORIGIN      
        1 gcgcaaagag cctgcgctca tgaacgcaga agcgcaggct tccttttacg ctgccccgga
       61 gcgcgcaggg ctcattcgtc ctccttttca gttaccgatg gaaacaaggc cgtgaagaca
      121 agtccttttt aatgtgctcc taaaagagac cgatccgaca aacgaagccc cggcacgttg
      181 tgatgccggg gcttcgtttg cggaaacacg aattttttaa aaatgccgtc tctctttgcc
      241 cctcatttca agaggcaaac tgaatttttc aatcgggcac tgttttttcc atttttaaaa
      301 tgggagcctc cgagccccct tggggctcgc tccacttcgt tccgctcgcc tacttccgaa
      361 cccatctaac cgcttcagaa tcgctcataa aagcttccaa gagcttcttc gctacccagg
      421 tctgaaaggc gcagaaacgg cggtttggag ccatttccga gagggcggaa aggggtgtgt
      481 ccagtcgtgc ccagaagatt tccgtttctc gtttcgcctc aaaatgcccc ttggttgagg
      541 gcttgagacg ggacgagaag taaaaagagc cccggcatct ttcgatgcct ggggctcctt
      601 ttttggaaaa ttcaatttgc ctttttggaa aatctaattt gccctacctt acttaagggt
      661 tcctcgcacg ctttagatca aagagacaac ggtgaccccg tcctctccct cgcagaggcc
      721 tccgcatttc aaaaaaggca aaaagcggga tacatcccac attcgctttt tacagccgcg
      781 ttctcatcga cgctctcaaa tgctctgtcc gcttgctcga cctgtggggg cgctttacct
      841 acccggtccg gcgctgtctc atccctctgc ttgcatagca ccgccgcttc gtgtaggata
      901 agggcagggg aaagcgtttc ttgagcaaag ggatgaggcc ggggcgcggg cgctgtcaag
      961 aggcggcctc gggccacaaa aaggagggtg cggcgatgaa aaaacggatc tgtgcttttt
     1021 ggttggcagc ggcgatggcc ctggggcttt ttggctgcgg ggaaacggcg caggggcagg
     1081 ggccggggcc gacggggggc ccggcggcgg acagcgggcg cggcgccttt ttggcggcgc
     1141 agcaggatca atatgacccg gcgctggtgc ccgcggtggg acgctatacg gtggcggagg
     1201 atttcagcaa cgtgatcaac tacagcgact gggatcgtgc gttcagcgac gaggcaaagc
     1261 agatgctgct gcaaaacggc tttgtggtgg tgggggacgg catggaagag ttttttagcc
     1321 tctatgagaa caaccgctat tccctgaccc cgaactttgt caccaccgac gccatgatgc
     1381 acacctatca tctgttcttc agccgcctgc tgaaaaatgt ggaacgggag catttttgcg
     1441 cc
//
LOCUS       DBMDYA010000717         1440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17092_length_1440_cov_1.122479, whole genome shotgun sequence.
ACCESSION   DBMDYA010000717 DBMDYA010000000
VERSION     DBMDYA010000717.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_1440_cov_1.122479"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="ACZPFK_13585"
     CDS             <1..260
                     /locus_tag="ACZPFK_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1407385.1"
                     /translation="VNTTYTLIKRCIQKRAVERREPRFICHALIPKEQVQRQETDELL
                     NKLFDGSADKLFASLLGREDLSPAQIERLRHMVEELEGKEK"
     gene            257..>1440
                     /locus_tag="ACZPFK_13590"
     CDS             257..>1440
                     /locus_tag="ACZPFK_13590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1407386.1"
                     /translation="MTLLQRSLAGGLMIAVVLAVRALAAERLPRRAFLILWEIALLRL
                     LLPWAAQSPFSLYALVRPAPTARGGAAVWAARLIPSSAASGQAAEAPAGTPWWRLLWI
                     VGCLVCILYFGVASLRWRRRFAESLPVEDGWARGWIKIQGLKRRVRLRQSDRVGTPLT
                     YGLLRPVILVPASMEWDDREQASYILCHELTHIRRWDPLTKLLLAAALCIHWFNPLVW
                     GMFLLANRDLELSCDEAVVRAFGEGSRAGYSMALLRQAERNSRPTPLCSGFGKSAIEE
                     RVEAIVKYRKTHFTATLCAALLVAAVALGLATTADPHRGAAGDRASSTPDAGSAAALF
                     QGDGALGQTPAVAFEHVELRYYEDNGWPYLHDAFTNRTGRTIRKLEYGMLAYDAEGAP
                     LQ"
BASE COUNT          234 a          442 c          513 g          251 t
ORIGIN      
        1 atgtgaacac cacctatacc ctcatcaaac gctgcatcca aaaacgcgcc gtcgagcggc
       61 gggagccgcg ttttatctgc catgcgctca tccccaaaga gcaggtgcag cggcaggaga
      121 ccgacgaact gctgaacaaa ctgtttgacg gctcggcgga caagctgttc gcctccctgc
      181 tggggaggga ggacctcagc cccgcgcaga tcgagcggct gcggcacatg gtggaggagt
      241 tggagggaaa agaaaaatga cgctgctgca gaggagcctg gccggcggcc tgatgattgc
      301 ggtggttctg gcggtccgcg ccctggcggc ggagcgcttg ccccgcaggg cctttttgat
      361 cctgtgggag atcgcgctgc tgcgcctgct gcttccctgg gcggcgcagt ctccgttcag
      421 cctctatgcg ctggtgcgcc ccgcgcccac ggcgcggggc ggcgcggcgg tttgggccgc
      481 ccggctcatt ccctcttccg ccgcctcggg gcaggcggcg gaggccccgg cggggacgcc
      541 ctggtggcgg ttgttgtgga tcgtggggtg cctggtttgc atcctctatt ttggggtggc
      601 atccctgcgc tggcggcgca ggtttgccga atctctgccg gtggaggacg gctgggccag
      661 gggctggatc aagatccaag gcctaaaacg acgggtgcgg ctgcggcaat cggaccgtgt
      721 cggcaccccg ctgacctacg gcctgctgcg cccggtgatc ctggtgccgg caagtatgga
      781 gtgggacgac agggaacagg cctcctacat cctctgtcac gagctgaccc atatccgccg
      841 ctgggacccg ctgacaaagc tgctgctggc ggcggcgctc tgcatccact ggtttaatcc
      901 gctggtctgg ggaatgtttc tactggcaaa ccgggacctg gagctatcct gcgacgaggc
      961 ggtggtgcgg gcctttgggg aggggagccg cgccggctac tcgatggccc tgctccgcca
     1021 ggcggagcgg aacagcaggc cgacgccgct gtgcagcggc tttggcaaaa gtgcgataga
     1081 agaaagggtg gaagcgattg tgaaatacag aaaaacgcat ttcacggcca ctctctgcgc
     1141 ggcactgctg gtggcggcgg tcgctctggg gctggccacg accgccgacc cgcacagagg
     1201 ggcggcgggg gacagggcgt cgtccactcc tgatgccggg tccgcggcgg cgctctttca
     1261 gggcgacggc gccctagggc aaacgccagc cgtggcattt gagcatgtgg agctgcgcta
     1321 ttatgaggac aacggctggc cctatctgca cgacgccttt accaaccgca cggggcgcac
     1381 catccgcaag ctggagtacg gcatgctggc ctatgacgcc gagggggccc cgctgcagat
//
LOCUS       DBMDYA010000718         1437 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17133_length_1437_cov_1.540419, whole genome shotgun sequence.
ACCESSION   DBMDYA010000718 DBMDYA010000000
VERSION     DBMDYA010000718.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17133_length_1437_cov_1.540419"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="ACZPFK_13595"
     CDS             complement(<1..1270)
                     /locus_tag="ACZPFK_13595"
                     /inference="COORDINATES: protein motif:HMM:NF019011.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HIR1407387.1"
                     /translation="MKVMIGAFAHESNDFCPHLTTREMFEFYEGEEVARHLPVADLFE
                     EAGIEVIPSIYATAESYGPVEEETFLHFERMILDTVRANPDLDGIWMFCHGAMNVQNI
                     GSGEYRLARDLRALVGENCVISFGMDLHGNLEEDFVNQVNIFRCYHTAPHTDQPDTYR
                     RTARALVEYLRHGYRLHPQMRKLPMIFPGEMAATTVEPFKSIIAYLDELERRPEVLCA
                     SCYIGFAWTDAERTSSTVAVVPAAPEYEPLCAELADDLARRVFARRREFGFEMTALRP
                     RETARASLCELPPPCCVTDMGDNPTAGTTGGGTVLLREYLAAVEAVGGGKRVLLAGIC
                     DPRAFAVCKAHQPGEEFDLSIGMDIDEVTRPVRVRAVYRGCHDVLGYKIATVPPIKRC
                     EAVTVSVGTVDVVLTDQAYAFTQKVNFSSCE"
BASE COUNT          244 a          504 c          461 g          228 t
ORIGIN      
        1 gctcacagga ggagaaattc actttttggg tgaacgcata ggcctggtcg gtgagcacca
       61 cgtccaccgt ccccaccgag acggtcactg cctcgcagcg cttgatgggg ggcacggtgg
      121 cgatcttgta tccgagcacg tcgtgacagc cgcggtagac cgcccgcacc cgcaccggcc
      181 gggtgacctc gtcgatgtcc atgccgatgg aaaggtcgaa ctcctccccc ggctgatggg
      241 ccttgcagac cgcaaaggcc cgggggtcgc agatgcccgc cagcaggacc cgctttcccc
      301 cgcccactgc ctccaccgcc gccaggtact cccgcagcag cacggtgccg ccgccggtgg
      361 tacccgcggt ggggttgtcg cccatgtcgg tcacgcagca ggggggcggc agctcgcaga
      421 gagaggcccg ggccgtctcg cgggggcgca gcgccgtcat ctcaaaaccg aactcccgcc
      481 tgcgcgcaaa gacccgccgg gccagatcgt ccgccagctc cgcgcagagc ggttcatatt
      541 ccggcgcggc gggcaccacc gccacggtgg aggaggtgcg ctcggcgtcg gtccaggcaa
      601 agccgatgta gcagctggcg cagagcacct cgggcctgcg ctccagctcg tccagatagg
      661 cgatgatgct cttgaacggc tctaccgtgg tggccgccat ctcaccgggg aaaatcatcg
      721 gcagcttgcg catctgcggg tgcaggcggt agccgtggcg cagatactcc accagcgcgc
      781 gcgccgtgcg ccggtaggtg tcgggctggt cggtgtgggg cgcggtgtga tagcagcgga
      841 agatgttcac ctggttcaca aaatcctcct ccaggttgcc gtgcaggtcc atgccgaacg
      901 agatcacgca gttttccccc accagggcgc gcaggtcccg ggccagccgg tactcgccgc
      961 tgccgatgtt ctgcacgttc atcgccccgt ggcagaacat ccagatgccg tccaggtccg
     1021 ggttcgcccg cacggtgtcc aggatcatcc tctcaaagtg gagaaaggtc tcctcctcca
     1081 ccggcccgta ggactccgcc gtggcgtaga tggaggggat cacctcgatg cccgcctcct
     1141 caaagagatc ggccaccggc agatggcgcg ccacctcctc cccctcgtag aactcgaaca
     1201 tctcccgggt ggtgaggtgc gggcagaagt cgttggattc atgcgcgaac gcgccgatca
     1261 tcactttcat ttcaggcctc cttttctttt tcccgtctcg ggacgggccc ggcctgcagc
     1321 cgggcgagga caaggtcggc gatcagcccc ggcagcacca gccccagcag cgtcaccacc
     1381 cccaccgtgc cgcccagggc ccagcccacc acaagggcga agcagtagag aagccag
//
LOCUS       DBMDYA010000719         1431 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17206_length_1431_cov_1.783459, whole genome shotgun sequence.
ACCESSION   DBMDYA010000719 DBMDYA010000000
VERSION     DBMDYA010000719.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17206_length_1431_cov_1.783459"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /gene="gatB"
                     /locus_tag="ACZPFK_13600"
     CDS             <1..1332
                     /gene="gatB"
                     /locus_tag="ACZPFK_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1407388.1"
                     /translation="LCCPICMGLPGTLPVLNQQVVESAVKMGHALNCRINGVTKQDRK
                     NYFYPDLPKAYQISQFDVPLCEGGYLDVLVGDEVRRIGVTRIHIEEDAGKLIHDDSFD
                     GTLCDYNRCGVPLIEIVSEPDLRSADEAKAYLETITTILLYLGISDAKMQEGSVRCDV
                     NVSVRPRGASEFGTRCEMKNVNSFGAVYRAIVYEAERQMEVLAAGGTIQQETRRWDDA
                     KGKSILMRSKEDAQDYRYFPDPDLLTFVVSDEKIAALKASLPELPTAKTLRYVKEFGL
                     QKVDAQLLSVNKARADFFDSCAALGRCEAKTAANWILGDIAKILNERRIEITDTALTP
                     EHLADMIVLIGKGTISNTAAKTVLEEIMERDVDPAQVVKEKGLAQISDASALSGIVDQ
                     VLADNPKAVADYKKGKVNVAGFLVGQCMKASRGQGNPATLRDMVTEAIEKL"
BASE COUNT          300 a          442 c          456 g          233 t
ORIGIN      
        1 ctttgctgcc ccatctgcat gggcctgccc ggcaccctgc cggtgctcaa ccagcaggtg
       61 gtggagagcg cggtgaagat gggccacgcc ctgaactgcc gcatcaacgg cgtcaccaag
      121 caggaccgca aaaattactt ttaccccgac ctgcccaagg cctaccagat ctcccagttc
      181 gacgtgccgc tgtgcgaggg cggctatctg gatgtgctgg tgggggatga ggtgcgccgc
      241 atcggcgtca cccgcatcca catcgaggag gacgcgggca agctgatcca cgacgactct
      301 tttgacggca ccctctgcga ttacaaccgc tgcggcgtgc ccctgatcga gatcgtctcc
      361 gagccggacc tgcgcagtgc cgacgaggcg aaagcgtatc tcgagaccat caccaccatt
      421 ttactgtatc tgggcatcag cgacgccaag atgcaggagg gcagcgtgcg ctgcgacgtc
      481 aacgtctcgg tgcgccccag gggcgccagt gagttcggca cccgctgcga gatgaaaaac
      541 gtcaacagct ttggcgcggt ctatcgcgcc atcgtctatg aggcggagcg tcagatggag
      601 gtgctggccg cgggcggcac catccagcag gagacccgcc gctgggacga cgccaagggc
      661 aaaagcatcc tcatgcgcag caaagaggac gcgcaggatt accgctattt ccccgacccg
      721 gacctgctca cctttgtggt gagcgacgag aagatcgcgg ccctcaaggc aagcctgccg
      781 gagctgccca ccgccaagac cctgcgctac gtcaaggaat tcggcctgca aaaggtggac
      841 gcccagctgc tcagcgtcaa caaggcgcgg gcggatttct tcgactcctg cgccgccctg
      901 ggccggtgcg aggcgaagac cgctgcaaac tggattctgg gcgacattgc aaagattctc
      961 aacgagcgcc gcatcgagat caccgacacc gccctcaccc ccgagcattt ggcggatatg
     1021 atcgtgctga tcggcaaggg tacgatctcc aacactgcgg ccaagacggt gctggaggag
     1081 atcatggagc gggatgtgga cccggcgcag gtggtaaaag agaagggcct ggcccagatc
     1141 agcgacgcct cggcgctgtc cggcatcgtg gatcaggtgc tggccgacaa ccccaaagcg
     1201 gtggcggatt acaaaaaggg caaggtgaac gtggcgggct ttctggtggg gcagtgcatg
     1261 aaagcgagcc gcggccaggg caaccccgcg accctgcggg acatggtcac cgaggccatc
     1321 gaaaagctgt aaagcgtgcc gtgccattgc tgaaaggcgc aggtgctctg gaataaaaat
     1381 agccgcaggc tgttttttcg gccatgtgac ccatccttga gccacggcca t
//
LOCUS       DBMDYA010000720         1430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17227_length_1430_cov_1.118886, whole genome shotgun sequence.
ACCESSION   DBMDYA010000720 DBMDYA010000000
VERSION     DBMDYA010000720.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17227_length_1430_cov_1.118886"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(791..1423)
                     /locus_tag="ACZPFK_13605"
     CDS             complement(791..1423)
                     /locus_tag="ACZPFK_13605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1407389.1"
                     /translation="MTVRRETEADRDRVEQITREAFYNLYVPGCVEHYLAHVLRGHRD
                     FVPELDLVLERDGRVIGSIMYTRAVLTDGAGAEKEVLTFGPVSVLPGFQRRGYGKLLM
                     EDSFERARALGYEAIVIFGSPANYVGRGFKSCKKYCVHAEDGAYPAAMMVKELRPGAL
                     EGRSWVYRDSPALHIDEQEAQRFDDGLEKMEKKWQPSQEEFYILSHATLQ"
BASE COUNT          217 a          524 c          435 g          254 t
ORIGIN      
        1 caccgtcctg cgccgcaggg gtgggcctgg catcttgagc cgccggggcg gtttgcacaa
       61 aaaacaccga aaggcaaagc ccgtttcagg gcgcggcccg cgcgccgccc cctgtcaggc
      121 ggcctgtgca ccccgcccgg atggtctgag cgtcccagcg gggtcagggc tgcggccggg
      181 gcggtttgca caaaaaacac cgaaaggcaa tacccatttc agggcgcggc ccgcgcgccg
      241 cccctgtcgg gtggcctgag cgccccgccc ttttgaactt cggattctcc aaaaagctgc
      301 cttggccttt ctcttttccg aacggccgga caggaaactc ccctcccggc ggagaaaggc
      361 ggggcggtgg gcagctgctc tttcgcgtgc ccgccgctcg cctgctgcgt gcccgccgtc
      421 tgcccgccgc tcccaacgga tcgttgcacg gggcgccgca ggtggaaagg cccggcttcc
      481 gccgggccta tgtgctcttt cctcaaaagt aaaacaagag gacgcgcccc cctgagagcg
      541 ttcgggccgg cgctttgccg gccccagggt ctccaggttg cttttcctct tgttcggggg
      601 cgaccgggcc aacgccccgg caaaaaatca ggagggacgg cgggcaagcc atttgcccgc
      661 cgtccctcct ctctccgtca cgaacagggt cggtcccccg ctctgccgca gccgcccttt
      721 tgaggcgccg cacggcgcca tccagctgca gcgcgcgttt cagccgcccg ccccgcacgc
      781 ccggcagcgg tcactggagc gtcgcgtggc tgaggatata aaactcctcc tggctgggct
      841 gccacttttt ctccatcttt tccaatccgt cgtcgaagcg ctgcgcctcc tgctcgtcga
      901 tgtgcagggc ggggctgtcc cggtagaccc agctccgccc ctccagcgcg cccggccgca
      961 gctctttcac catcatggcc gccggatagg cgccgtcctc ggcgtggacg caatactttt
     1021 tgcagctttt gaagccgcgc cccacataat tggccgggct gccgaagatg acgatcgcct
     1081 catagcccag cgcccgtgcc cgctcaaacg agtcctccat caacagcttg ccgtagcccc
     1141 tgcgctgaaa gcccggcagg acgctgaccg ggccaaaggt cagcacctct ttttccgctc
     1201 cggccccgtc ggtcagcacg gccctggtgt acatgatgct gccgatcacc cggccgtcgc
     1261 gctccagcac caggtccagc tcgggcacaa aatcccggtg gccgcgcagg acgtgggcga
     1321 ggtagtgctc cacacagccg ggcacataca ggttgtaaaa agcctcgcgg gtgatctgct
     1381 ccacccggtc gcggtcggct tccgtctccc gtcgcacggt gatgttttct
//
LOCUS       DBMDYA010000721         1428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17257_length_1428_cov_1.510927, whole genome shotgun sequence.
ACCESSION   DBMDYA010000721 DBMDYA010000000
VERSION     DBMDYA010000721.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17257_length_1428_cov_1.510927"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(55..927)
                     /locus_tag="ACZPFK_13610"
     CDS             complement(55..927)
                     /locus_tag="ACZPFK_13610"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR1407390.1"
                     /translation="MRIYKLFEGGGPVFSLEVFPPKKTSSIESMYRTLAELAELKPDY
                     ISVTYGAGGTAAGLSCELSAHIKHRLGIEPLAHLTCINSDRGQVGQVLDRLQAERIEN
                     VLALRGDRVPGLEPKTDFFHADELMRFIDRRYDLSLAGACYPEGHPDSESLEADARNL
                     RRKQEAGAKHLVSQLFFDTDAYFRFLDRAAAAGVTLPVQPGVMPIVKLAQIQRTVALS
                     SASLPASFSKMITRYEYDPDGLYKAGIQYAVSQCLALLAGGAPGVHLYTMNNADVARR
                     VYQGIRGALGREEG"
BASE COUNT          260 a          465 c          423 g          280 t
ORIGIN      
        1 ggtaacggca gatctccgcc ctgttcagag gggccttcag cgcaagcttc atgctcagcc
       61 ctcttcccgt cccagcgcgc cgcggatgcc ctgatagacc cgccgggcca cgtccgcgtt
      121 gttcatggtg tagagatgca cgcccggcgc gccgcccgcc agcagggcca ggcattggct
      181 gaccgcgtac tggatgcccg ctttgtacag gccgtcgggg tcgtactcgt agcgggtgat
      241 catcttggag aaagaggcgg gcaggctggc ggagctcagc gccaccgtgc gctggatctg
      301 cgccagcttg acgatgggca tcacccccgg ctgcaccggc agggtgaccc ctgcggcggc
      361 ggcccggtca agaaagcgga aataggcgtc ggtgtcaaaa aaaagctggc tcaccagatg
      421 cttcgccccc gcctcctgct tgcggcgcag gttccgggca tccgcttcta ggctctcgct
      481 gtccgggtgc ccctcggggt agcaggctcc ggccagcgag aggtcgtacc gcctgtcaat
      541 aaaccgcatc agctcgtcgg catggaaaaa atcggtcttc ggctccagcc ccggcacccg
      601 gtcgccccgc agggccagca cgttttcgat ccgctccgcc tgcagccggt ccagcacctg
      661 ccccacctgg ccgcggtccg agttgatgca ggtgagatgg gcgagcggct cgatgcccag
      721 gcggtgcttg atgtgggcgg acagctcgca ggagagcccc gccgccgtgc cgcccgcgcc
      781 gtaggtgacg ctgatgtaat ccggcttcag ctcggccagc tccgccaggg tgcggtacat
      841 gctttcgatg gagctagtct ttttcggcgg aaagacctcc agcgaaaaga cgggcccgcc
      901 gccctcaaac aatttatata tcctcatatg cttttccccc attcccccgc ggccgcgcct
      961 ttctccgccc gtcacggcgc gcggacgcaa cgatcgccgt ctctatctgc ccgcttatcc
     1021 tcggcttttc ggcgcaaaag cgggcggccc cgcccgcaaa ctttcgcacc aaagaccgcc
     1081 ggggcgggac ataatcccaa atccctgtaa aaagacagcc tccgctttca gccgggcccg
     1141 ggccccgttt ggatacggct gtttttcatt ataaagccgc gcgcccggct ttgtaaacct
     1201 tttttcgccg cgatcagccg ggccgcggca ttttctcaat cgaaagagca atttttcatt
     1261 gtttttttat cgaaaataag gtattctgga atcagtaaaa aatgaaagct tacggcgtca
     1321 gcgagagggg ggtgcggatg gacaaaagta gagccccggc cttttgggcg gatatggaca
     1381 acgaattcta taagcatgtc ctcacctcga tggaaaaccc gtttttca
//
LOCUS       DBMDYA010000722         1426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17281_length_1426_cov_1.860377, whole genome shotgun sequence.
ACCESSION   DBMDYA010000722 DBMDYA010000000
VERSION     DBMDYA010000722.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_1426_cov_1.860377"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            233..499
                     /locus_tag="ACZPFK_13615"
     CDS             233..499
                     /locus_tag="ACZPFK_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR1407391.1"
                     /translation="MENRLCVVSIIVEDRASAAKINDLLHECGGYIVGRMGLPYRERG
                     VSVICVIIDAPGDVISALSGKLGMLPGVTAKTMTSKAQLRPDAP"
     gene            574..>1426
                     /locus_tag="ACZPFK_13620"
     CDS             574..>1426
                     /locus_tag="ACZPFK_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1407392.1"
                     /translation="MKKLIALVLSLALAASALAGCGASGSSSSAAASLPASSGAASSS
                     AAEKVPVEPADIRIAGLKGPTSMGMVKLMEDAENGEAANNYTFTIAGSADEVTPKLIQ
                     GEFDIAAVPANLASVLYNNTEGAVKLLAVNTLGVIYIVEKGDSVQSLADLKGKTIYAT
                     GKGSTPEYALNYILSENGIDPAADVTVEWKTEPTEVVQLLAQSENAVAMLPQPYVTVA
                     QTQVEGLRVAVDLNQAWSELDNGSLFLTGVLVVRADFAEQYPDQLAAFLQEYQASTEW
                     VNANVPEA"
BASE COUNT          259 a          474 c          450 g          243 t
ORIGIN      
        1 gtcgctgcga aaatcagcaa gtttaccggc gtccccaatg ggcgccggat atccccatat
       61 gattttccgc gcctttccgg gcgcggattt tttgttgctt tgacggctgc gtctcccgcc
      121 gatgggcggc agccggagca gcaggcgaag agagcggtct ttgccccgca gcgaagcgcc
      181 gctctgccgc gtccggcgaa gctgaagcaa aagaaagggg gaacagaggc ccttggaaaa
      241 ccggctttgc gttgtcagca ttatcgtgga ggacagggcg tccgcggcga agatcaatga
      301 tctgctgcat gagtgcggcg gttatatcgt cggccgcatg gggctgccct atcgcgagcg
      361 gggcgtctcg gtgatctgcg tgattatcga cgcgccggga gacgtcatca gcgccctttc
      421 gggcaagctg ggcatgctgc ccggcgtcac cgcaaagacg atgacctcca aggcgcagct
      481 gcgccccgac gccccgtgac cgcccggcgg ccacaacacc ccggcccggc gttcgctttt
      541 gtccgggcgc gcacatgaaa aggagaaacc cacatgaaaa aactgatcgc actcgtgctc
      601 tccctcgccc ttgccgcctc cgcgctggcg ggctgcggcg catctggcag ctcgtcctcc
      661 gcggccgcct cgctgcccgc ctcctccggc gcggcctcct cttccgctgc tgaaaaggtc
      721 cccgtcgagc ccgcggacat ccgcatcgcc gggctgaaag gccccacctc catggggatg
      781 gtcaagctga tggaggacgc cgaaaacggc gaagccgcca acaactacac gttcaccatc
      841 gccgggtccg ccgacgaggt cacccccaag ctgattcagg gcgaattcga cattgccgcc
      901 gtgcccgcca atctggcctc ggtgctctac aacaacaccg agggcgccgt caagctgttg
      961 gcggtcaaca ccctcggcgt catctacatc gtcgaaaagg gcgacagcgt gcagtcgctg
     1021 gccgacctga agggcaagac catctatgcc accggcaagg gctccacccc cgagtatgcg
     1081 ctcaattata tcctcagcga aaacggcatc gacccggccg ccgacgtgac cgtggagtgg
     1141 aagaccgagc ccaccgaggt ggtgcagctg ttggcgcagt ctgagaatgc tgtcgccatg
     1201 ctgccccagc cctatgtcac cgtggcccag acccaggtgg aggggctgcg ggtcgccgtg
     1261 gatctgaacc aggcctggag cgagctggac aacggcagcc tgtttttgac cggcgtgctg
     1321 gtggtgcgcg cggactttgc cgagcagtat cccgatcagc tggcggcgtt tctgcaggag
     1381 taccaggcgt ccaccgagtg ggtcaacgcc aatgtccccg aggctg
//
LOCUS       DBMDYA010000723         1424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17308_length_1424_cov_2.227513, whole genome shotgun sequence.
ACCESSION   DBMDYA010000723 DBMDYA010000000
VERSION     DBMDYA010000723.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17308_length_1424_cov_2.227513"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="ACZPFK_13625"
     CDS             complement(<1..586)
                     /locus_tag="ACZPFK_13625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR1407393.1"
                     /translation="MDLYQTDPEFAERFEAFALEEVAQEAGRQLGETTRWTATLAALL
                     GCQGVEAFRAALPRALDAGVSAVAVKEIVYQAVDYLGLGRVLPFLTAANDVLLARGVE
                     LPLPGQATTTAEDRLEKGAAAQAEIFGERMKEAWKTSTINCWLAANCFGDYYTRTGLD
                     LRQREMITFCFLAAQGGCEPQLTAHACGNMNLGND"
     gene            complement(631..>1424)
                     /locus_tag="ACZPFK_13630"
     CDS             complement(631..>1424)
                     /locus_tag="ACZPFK_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1407394.1"
                     /translation="VVVATKFLPRTQEEVDAGVSGRQHIQRLLDKSLQNLGMDYVDLY
                     ICHMWDHQTPLYDVMEGLDRVVKAGKARYIGISNCFAWQLAKANALAEREGFAKFISV
                     QGHYNLIFREEEREMAGLCREEGIAMTPYSALAGGRLSKRPGESSKRLAEDSYARMKY
                     DGTADRDGPVIRRVAELADRRGVSMTEISLAWLLSKTTVPVVGATKLHHIEGAARAVD
                     LTLSPEETAYLEELYTPHPLVGVMAQNTAAAAGENHVWSTGSQKL"
BASE COUNT          228 a          513 c          409 g          274 t
ORIGIN      
        1 tgtcgttgcc caggttcata ttgccgcacg cgtgggcggt gagctgcggc tcacagccgc
       61 cctgggccgc caaaaagcaa aaggtgatca tctcacgctg cctcagatcg aggccggtgc
      121 gggtgtagta gtccccaaag caattggccg ccaaccagca gttgatggtg ctcgttttcc
      181 aggcctcttt catgcgttcg ccgaaaattt ccgcctgggc cgccgcacct ttctccagcc
      241 ggtcctcagc ggtggtcgtg gcctgcccgg gcagcggcag ttcgacgccg cgggccagca
      301 gaacatcgtt ggccgcggtc aaaaacggca gcacccggcc aaggcccaga tagtccaccg
      361 cctggtagac gatctccttt acagccaccg cggagacgcc cgcgtccagc gcgcgaggca
      421 gcgcggcgcg gaaagcctcc accccctggc agcccagcag ggccgccagg gtcgccgtcc
      481 agcgcgtcgt ttctcccagc tgccgcccgg cctcctgggc cacctcttcc aatgcaaacg
      541 cctcaaagcg ctccgcaaat tcagggtcgg tctgatacaa gtccattcca gtcccctctc
      601 tttccccgct gtgattgtcg ttgctcccga tcagagtttt tgactgccgg tggaccagac
      661 atggttttcc cccgcggccg ccgctgtgtt ctgcgccatc acgcccacca gcggatgcgg
      721 ggtgtacagc tcctccagat aggcggtttc ctccggcgag agcgtcaggt ccaccgcccg
      781 ggccgcgccc tctatgtggt gcagcttggt cgcgcccacc acgggcaccg tcgtctttga
      841 cagcagccag gcgagagaaa tttcggtcat ggacaccccg cggcggtccg ccagctcggc
      901 gacgcgccgg atgaccggcc cgtcccggtc cgccgtgccg tcatatttca tgcgggcgta
      961 gctgtcctcc gccagccgct tggagctttc tcccggacgc tttgacagcc gtccgcccgc
     1021 gagggcgctg tacggcgtca tggcgatgcc ctcctccctg cacagcccgg ccatctcccg
     1081 ctcttcctcc cggaagatca ggttgtaatg cccctgtacg gagatgaact ttgcaaaccc
     1141 ctcccgctcg gccagagcgt tcgcctttgc cagctgccag gcaaaacagt tggagatgcc
     1201 gatatagcgc gccttgccgg ccttcaccac ccggtccagg ccctccatca cgtcgtacag
     1261 cggcgtctga tggtcccaca tatggcagat gtacaggtcg acatagtcca tccccaggtt
     1321 ttggaggctt ttgtccagca gccgctggat atgctgcctg ccgctgacac ctgcgtcgac
     1381 ctcctcctgg gtgcggggca aaaacttggt cgccaccacc acct
//
LOCUS       DBMDYA010000724         1424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17316_length_1424_cov_1.032502, whole genome shotgun sequence.
ACCESSION   DBMDYA010000724 DBMDYA010000000
VERSION     DBMDYA010000724.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17316_length_1424_cov_1.032502"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="ACZPFK_13635"
     CDS             <1..682
                     /locus_tag="ACZPFK_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1407395.1"
                     /translation="EIAKLRLYGEDEGQAQSARRVLVYVLREALKLLNPFMPFITEEI
                     YRALPGSSESIMVSAWPVWREELCFPQDEEGFTNLVALIKAVRAVRAEMGVHPAKKTR
                     IFIQTGSPADFETGRAYLEKFAAASEIEIGEAFSGDASGSVQVVTDKARAFIPMMELV
                     DREKELARLEKELASCEKEIASAGAKLNNQGFVAKAPAQVVENVRAQLARAQDKKAHI
                     QASIQALG"
     gene            878..>1424
                     /locus_tag="ACZPFK_13640"
     CDS             878..>1424
                     /locus_tag="ACZPFK_13640"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HIR1407396.1"
                     /translation="MTDREAIEWLHGRRRLGEEKNPDTMRRLMGLLGDPQDQLAFAHI
                     AGTNGKGSVAVMLASILQQAGVRTGLFLSPFVEEFGERITVDGAPLPGGALGEAAARA
                     QVAAEHLEKDGLYATEFELVTAVGFLCFLRQGCDLVCLEAGLGGSRDATNIVKNTKVA
                     CLTCIGLDHTELLGDTVEAIAE"
BASE COUNT          258 a          435 c          521 g          210 t
ORIGIN      
        1 cgagatcgcc aagctgcgcc tttacggcga ggacgagggg caggcccaga gcgcccgccg
       61 cgtgctggtg tatgtgctgc gcgaggcgct gaagctgctg aacccgttca tgccgttcat
      121 caccgaggag atctaccgcg ccctgcccgg cagcagcgag agcatcatgg tctccgcctg
      181 gcccgtctgg cgggaggaac tctgcttccc acaggacgag gagggcttca ccaatttggt
      241 ggcgctcatc aaggcggtgc gggccgtgcg cgccgagatg ggggtccacc cggcgaaaaa
      301 gacccgcatc tttatccaga ccggcagccc ggcggatttt gagaccggcc gcgcttatct
      361 ggaaaaattc gccgccgcca gcgagatcga gatcggcgag gctttcagcg gcgacgcctc
      421 cggcagcgtg caggtggtca ccgacaaggc acgggcgttc atccccatga tggagctggt
      481 ggacagggaa aaagagctgg cccgcctgga aaaggagctg gcaagctgcg aaaaggagat
      541 cgcctctgcg ggcgccaagc tgaacaacca gggctttgtg gccaaggcgc ccgcccaggt
      601 ggtggagaat gtgcgcgccc agttggccag ggcccaggac aaaaaggccc acatccaggc
      661 ctccatccag gcgctggggt gagctgagcg cagggagggc gtgggcttgc ggcgggcgct
      721 ttttcggccg cggagagcgg ataaaagagc gccgcctgcg gcccggccgt gcggcggccg
      781 cgctgaaaaa cgccccgcag agggaccgcc ggttttgcac cgcggtccct ctgccgctgt
      841 gcggcaaaat gatggaagaa aagaggaggg acgtgccatg accgaccggg aagcgatcga
      901 gtggctgcac ggccgcaggc ggctgggcga agaaaagaac cccgacacca tgcggcggct
      961 gatgggcctg ctgggcgacc cgcaggacca actggcgttc gcgcacatcg cgggcaccaa
     1021 cggcaagggg agcgtggcgg tgatgctggc ctccattttg cagcaggcgg gggtccgcac
     1081 cgggcttttc ctctccccct ttgtggagga gttcggcgag cgcatcaccg tggacggcgc
     1141 gcctctgccc ggcggcgcgt tgggcgaggc ggcggcgcgg gcgcaggtcg cggcggaaca
     1201 tctggaaaaa gacggccttt acgccaccga gttcgagctg gtgacggcgg ttggcttcct
     1261 ctgctttttg cgccagggct gcgatctggt ctgcctggag gcgggcctgg gcggcagccg
     1321 ggacgccacg aatatcgtca aaaacaccaa ggtggcctgc ctgacctgca tcggcctgga
     1381 tcacaccgag ctgctgggcg acacggtgga ggccatcgcg gagg
//
LOCUS       DBMDYA010000725         1422 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17345_length_1422_cov_1.429220, whole genome shotgun sequence.
ACCESSION   DBMDYA010000725 DBMDYA010000000
VERSION     DBMDYA010000725.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17345_length_1422_cov_1.429220"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="ACZPFK_13645"
     CDS             complement(<1..1045)
                     /locus_tag="ACZPFK_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1407397.1"
                     /translation="MNYTQENIRNLLISGHGGSGKTSLVEALVYYTKGTDRLGRTDDG
                     NTVCDFDPEEIRRKASLSLALASAEYSDTKLNLIDVPGLFDFELGIHEGMPAADGAVI
                     CVSARDGLEVGSKKAYKLAHKYNKPTMFYVSKIDVENADFYKVFEELKAEFGPSICPV
                     VVPVEQAGGRIYINLIKMEAYSYVNGAAHTVPMPSFGHRLDGLVEAMSEAVAETDEEL
                     MEKFFSGEAFTQEELQRGVKAGIKSGSITPVFCGSSTKLDCIDAMLHYMKHLMPCPAE
                     AGAVTGENADGEPVELACDAAGPLAVYAFKTVADPFVGKLSYVKVLSGVLKADSPVVN
                     SRTGESERLGKLLF"
     gene            1139..>1422
                     /locus_tag="ACZPFK_13650"
     CDS             1139..>1422
                     /locus_tag="ACZPFK_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407398.1"
                     /translation="MKALICKKSPISKRIRSLLFVHLAQIKNQKSTLGDKKTDPGACA
                     KVGPYHYFALPGKARKRGFARRGRTRRRRRAGTATGLRPRSAPRFYSGRIP"
BASE COUNT          268 a          446 c          431 g          277 t
ORIGIN      
        1 tgaagagcag cttgcccagc cgctccgact cgccggtgcg gctgttgacc accggcgaat
       61 ccgccttgag cacgcccgag agcaccttga catagctcag cttgcccaca aaggggtcgg
      121 ccacggtctt gaacgcgtag accgccaggg ggcccgccgc gtcgcaggcc agctccaccg
      181 gctcgccatc cgcgttctcg cccgtcacgg cgcccgcctc ggcggggcag ggcatcagat
      241 gcttcatata gtgcagcatt gcgtcaatgc agtcgagctt tgtggagctg ccgcagaaca
      301 ccggggtgat gctgcccgac ttgatgccgg ccttgacgcc gcgctgcagc tcctcctggg
      361 tgaacgcctc gccggagaaa aatttctcca tcagctcctc gtcggtctcg gcgaccgcct
      421 cgctcatggc ctctaccagg ccgtcgagcc ggtggccgaa gctcggcatc ggcacggtgt
      481 gggccgcgcc gttgacatag ctgtaagctt ccatcttaat cagattgatg tagatgcggc
      541 cgccggcctg ctccaccggc acgaccaccg ggcagatcga ggggccgaac tcggccttga
      601 gctcctcgaa cactttatag aaatcggcgt tctcgacgtc gatcttggag acgtaaaaca
      661 tggtgggctt gttgtatttg tgggccagct tgtaggcctt tttgctgccg acctccagcc
      721 cgtcgcgggc ggagacgcag atcacggcgc cgtcggcggc cggcatcccc tcgtggatgc
      781 ccagctcaaa gtcgaacagg ccggggacgt cgatcaggtt cagcttggtg tcgctgtact
      841 cggccgaggc cagggccagc gagagggagg ccttgcggcg gatctcctcc gggtcaaagt
      901 cgcagacggt gttgccgtcg tccgtgcgcc cgagccggtc ggtgcctttc gtataataga
      961 ccagcgcctc gacgagactt gtcttgccgg aaccgccgtg gccggagatc agcagattgc
     1021 ggatgttctc ctgtgtgtag ttcatactct taggaccccc ttaacaaagt tggctgccgc
     1081 acctagccgt gcgtgccgac acgaatcata tcaaaatttt aacacatcaa aatctgtttt
     1141 gaaagcgcta atctgtaaaa aatcaccgat cagcaagaga attcgcagcc ttttatttgt
     1201 gcatttagca caaataaaga accaaaaatc gacgctgggc gataaaaaga ccgaccccgg
     1261 cgcgtgcgcc aaggtcggtc cttatcacta ttttgcgctg ccgggcaagg cccgcaagag
     1321 gggatttgcg cggcgcggcc gcacacggcg ccgccggcgg gcagggacgg cgacgggcct
     1381 ccggccacga tccgcgcctc gtttttacag cgggaggatt cc
//
LOCUS       DBMDYA010000726         1418 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17397_length_1418_cov_1.746393, whole genome shotgun sequence.
ACCESSION   DBMDYA010000726 DBMDYA010000000
VERSION     DBMDYA010000726.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17397_length_1418_cov_1.746393"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="ACZPFK_13655"
     CDS             <1..363
                     /locus_tag="ACZPFK_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407399.1"
                     /translation="VEKLEYYETAAGKEGWYELKGDSFGPASGFPLMDATSRFRVTFK
                     DAGKVAFSVKIVTVEDPTDILSGSYTFTGVAKGAEPTPTPSATPAPTAAPSPAPTASP
                     APAPAPAPGRANPVTGVA"
     gene            529..1308
                     /locus_tag="ACZPFK_13660"
     CDS             529..1308
                     /locus_tag="ACZPFK_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1407400.1"
                     /translation="MKRTDGKKTFDPGSSSKSRKRKRLFALIGLGALLCLGLLALALP
                     RQKQPALSSDSAFVPVSVGQASPRPFVSRAVSEAHAANSDVVGWLTLEGCEIDEPVLQ
                     SFDNDYYLRRNEAGEEDVWGCYFLDYINRNDGTALPDKVSIIYGHSVGDTAEGERFCA
                     LKRYRTADFALQNPTFGFSLLYEEDCWQIFSACDIPISIDYIDPNPSREKYQQTLDYM
                     LEHSYVDFGVEVGPSDHILILSTCTSDESVRFVVAAKPVQP"
BASE COUNT          287 a          454 c          390 g          287 t
ORIGIN      
        1 gtggaaaagc tcgagtacta tgagaccgcc gccggcaaag agggctggta cgagctgaaa
       61 ggcgactctt tcggccctgc ctccggcttc cccctgatgg atgccaccag ccgcttccgc
      121 gtcacgttca aggacgcggg caaggtcgct ttcagcgtca agatcgtgac cgtggaggat
      181 cccaccgaca tcctctcggg aagctatacc tttaccggcg tggccaaggg cgcagagcct
      241 acccccacgc cgtcggcaac cccggctccc acggccgccc cctctccggc tcccaccgct
      301 tctcccgcac cggcccccgc acctgctccc ggcagggcga accccgtcac cggcgtggcc
      361 tgacagatca ttttttaacg cagacccgag agggcggaca ggcgtccgcc ctctttcttt
      421 atcttgacgg cctcctttcc cgccgctgcg cggcaaggga aacggagagc cgaaaaagca
      481 ggagcaccgc ttttgttttt taccagtgaa agaggagatg gacaaaattt gaaaaggaca
      541 gacggcaaaa agacctttga tccgggctca agcagcaaga gcagaaaaag aaaacggctt
      601 tttgcgctca tcggcctggg ggccctgctc tgcctcgggc ttctggcgct tgcgctcccg
      661 cggcaaaaac agcccgccct ttcatccgac agcgcttttg tgccggtcag tgtggggcaa
      721 gccagcccgc gtccctttgt cagcagggcc gtatcggagg cccatgcggc aaacagcgac
      781 gtggtgggct ggctgaccct ggagggctgc gagatcgacg aacccgttct tcaaagcttt
      841 gacaacgact attatctgcg cagaaacgaa gccggtgaag aagatgtttg gggctgctat
      901 tttctggact atatcaaccg caacgacggc accgccctgc ccgacaaggt ctcgatcatc
      961 tatggccact ctgtgggcga cacggcggag ggcgagcgct tttgcgcgct caagcgatac
     1021 cggaccgctg attttgccct ccagaatccc acctttgggt tctccctgct ctatgaggag
     1081 gattgctggc agatcttctc cgcctgcgac attcccattt ccatcgacta catcgacccc
     1141 aacccctcaa gggaaaaata tcagcagacg ctggactata tgctggaaca cagctatgtg
     1201 gattttggcg tggaggtcgg cccctcggac cacatcctga tcctttcgac ctgcaccagc
     1261 gacgaatcag tgcggtttgt cgtggccgcc aagcccgtcc agccctgaga ggccggcctt
     1321 tctttttctc cgccgttgcc cttttctccg ccgcggcgtt ataatgataa aaaacgcggt
     1381 ccacatcctg cttttttcag aaaggggcgc aattagat
//
LOCUS       DBMDYA010000727         1413 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17467_length_1413_cov_1.432165, whole genome shotgun sequence.
ACCESSION   DBMDYA010000727 DBMDYA010000000
VERSION     DBMDYA010000727.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17467_length_1413_cov_1.432165"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            228..674
                     /locus_tag="ACZPFK_13665"
     CDS             228..674
                     /locus_tag="ACZPFK_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1407401.1"
                     /translation="MERGEAQLNRFLVEVFHDVMRLEEQELLKGPFKNLSVSEMHVIE
                     AACDGQDKGSNSMAALAARLRVSAGTLTIAVKTLEQKGYLLRERSRSDRRRVLVTPTS
                     LGHKAYEVHRQFHERLVGAVAGRLSPAGMDALADALSTLHRFFDSL"
     gene            749..>1413
                     /locus_tag="ACZPFK_13670"
     CDS             749..>1413
                     /locus_tag="ACZPFK_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1407402.1"
                     /translation="MAVRIITDSTADFSAQEARALGVSVVPLNVNFGPDEVYRDGVDL
                     DPQEFYTRLAAAEKLPTTSQPSPELFLSIFEEAKEAGDEVVAVLLSGPLSGTVQSAGL
                     ARGLCGYEEHIFVVDSLTATLGLKLLVQRALALRDEGRTGAEIAAQLDEEKHRIRLYA
                     VVDTLKYLQKGGRLSAAVAVAGTLLGIKPVITIQDGKVGVAGKARGLPGAYVTIFKLI
                     TDE"
BASE COUNT          255 a          460 c          446 g          252 t
ORIGIN      
        1 tttttctccc tccctctttt tgcgctctgc tcaaaacatg tcttccgtcc ctccctcaag
       61 cgggacggtc cgataaccgt attatagctt tcgcacccac acgcgtcaaa ccgcgccgca
      121 aaatagtttg aagttcaaaa cttcttgaca gacattcctc ggggtggtat actgccccat
      181 gttctattgc tttgaagttc aaaataatat ggaggggagg cggcgctatg gaacgcggcg
      241 aggcccagct caaccgcttt ttggtggagg tctttcacga cgtgatgcgg ctggaagagc
      301 aggaactgct caagggccct ttcaaaaacc tgtcggtgag cgagatgcat gtcatagaag
      361 cggcctgcga cgggcaggat aagggcagca acagcatggc tgcgctggcc gcgcggctgc
      421 gggtgtccgc gggcacgctg accatcgcgg tgaagaccct ggagcaaaag gggtatctgc
      481 tgcgcgagcg cagccgcagc gaccggcgcc gggtgctggt gacgcccacc tcgctggggc
      541 acaaggccta cgaggtgcac cggcagttcc acgagcggct ggtgggcgcg gtggccgggc
      601 gcctctcccc tgccggaatg gacgcccttg cggacgccct ctccaccctg caccgttttt
      661 ttgacagcct gtgacaaccg cccggccccg ccgcccacag cggcgggcgg ccccggagcc
      721 cctctcaaag aaaggattgg atcattttat ggcagttcgc atcatcaccg attccaccgc
      781 ggatttctcc gcccaggagg cccgggccct cggcgtctcc gtcgtgcccc tgaacgtcaa
      841 tttcggcccc gacgaggttt accgcgacgg cgtcgacctg gacccgcagg agttttacac
      901 ccgtctcgcg gcggctgaaa aactgcccac caccagccag cccagccccg agctgttcct
      961 ctcgatcttt gaagaggcca aagaagcggg cgacgaggtg gtggcggtgc tgctctccgg
     1021 cccgctgtcg ggcacggtgc agagcgccgg gctggcgcgc ggcctctgcg gctatgagga
     1081 gcacatcttc gtcgtcgaca gcctgaccgc caccctgggg ctgaagctgt tggtacagcg
     1141 cgcgctggcg ctgcgggacg aggggcgcac cggcgcggag atcgccgcgc agctggacga
     1201 ggaaaagcac cgcatccgcc tctacgcggt ggtggacacc ctgaaatatc tgcaaaaagg
     1261 cggccgcctc tccgccgctg tggcggtggc cggcaccctg ctgggcatca agccggtcat
     1321 caccatccag gacggaaagg tcggggtggc gggcaaagcg cgcggcctgc ccggcgccta
     1381 tgtcaccatt ttcaagctca tcaccgacga gga
//
LOCUS       DBMDYA010000728         1410 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17507_length_1410_cov_1.306341, whole genome shotgun sequence.
ACCESSION   DBMDYA010000728 DBMDYA010000000
VERSION     DBMDYA010000728.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_1410_cov_1.306341"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    248..506
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            630..1382
                     /locus_tag="ACZPFK_13675"
     CDS             630..1382
                     /locus_tag="ACZPFK_13675"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1407403.1"
                     /translation="MIQIENLTKVFKSGGGEVTALNNVSFAIEDGDIFGIIGMSGAGK
                     STLLRCLSMLERPTGGRILQDGADLAALRGKALIEAHRRTGVVFQGYNLLMQKSVRQN
                     VAFPLRLAGAKKAQIAPRVDELLDLVGLADKTDAYPAQLSGGQRQRVAIARALATSPQ
                     VLLCDEPTSALDPLTTKQVLALLRDINRKLGVTIVIITHEINVVRAICNKVAVIDAAQ
                     FAEGGRTDEIFDRPKSSITRLLLGTEGGLKHA"
BASE COUNT          276 a          449 c          453 g          232 t
ORIGIN      
        1 gacgcccgcc cctctgatcc ccccggcggc gttcccgccg ccggagcagg aggagggctt
       61 ttccaccccc tccaccgggc ggcaagacga tcttttttaa agccgtctct gtgatcccgc
      121 ggcaaaaagc gctttatttt tgtgcagttt tgccaagaaa gccggagatt ttttcaaaaa
      181 agtttgactg ttttttcccc gttgacaatg ttccgccccg gtgtataatg aaatcacaac
      241 cgaacagcaa aaggcgcaga tgagaagagt aagtatccgc cgctttcaca gagagccccc
      301 gatctggtgc aagagggcaa agcgaacgat gccgaacatg gtctcggagc tgcgcgggcg
      361 agccgaaagg ttagtccgcg acgggcccac ccgttacagt ggcaggctat ggacggcatc
      421 cgccggagta gctgcagagg cccgggcggc gcaagcgccg ggcgaaccaa ggtggtaccg
      481 cgggagcata tgcctccgtc cttgaagtga ttcaaggacg ggggcttttt tatttgccct
      541 gcggggcggc agcctgatcc gaccgcccgc ttcgccgccg aggacacgcg gcccggcggc
      601 tttttacagc ttttgaggag gcgcagaaca tgatccagat cgagaacctg accaaggtct
      661 tcaaatccgg cggcggcgag gtgaccgcgc tgaacaacgt cagcttcgcc attgaggacg
      721 gcgacatctt cggcatcatc ggcatgagcg gcgcgggcaa atccaccctg ctgcgctgcc
      781 tctcgatgct ggagcgcccc accggcggcc gcattctgca ggacggcgcc gacctcgccg
      841 cgctgcgcgg caaagcgctg atcgaggcgc accgcaggac gggcgtggtc tttcaggggt
      901 acaacctgct gatgcagaag tcggtgcggc agaacgtggc tttcccgctg cggctggcgg
      961 gcgcaaaaaa ggcgcagatc gccccccgcg tggacgaact gctggacctg gtggggctgg
     1021 cggacaagac ggacgcctat ccggcgcagc tctcgggcgg gcagcgccag cgggtggcca
     1081 tcgcccgggc gctggccacc agcccacagg tgctgctctg cgacgagccc accagcgccc
     1141 tggaccccct caccaccaag caggtgctgg cgctgctgcg ggacatcaac cgcaagctgg
     1201 gggtcaccat cgtcatcatc acccacgaga tcaacgtggt gcgggccatc tgcaacaagg
     1261 tggcggtcat cgacgccgcc cagttcgccg agggcgggcg cactgacgag atcttcgacc
     1321 gccccaagag cagcatcacc cggctgctgc tgggcaccga gggagggctg aaacatgctt
     1381 gagacgatca tcggcctcaa agacgtgctg
//
LOCUS       DBMDYA010000729         1410 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17510_length_1410_cov_1.182582, whole genome shotgun sequence.
ACCESSION   DBMDYA010000729 DBMDYA010000000
VERSION     DBMDYA010000729.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17510_length_1410_cov_1.182582"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="ACZPFK_13680"
     CDS             <1..859
                     /locus_tag="ACZPFK_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407404.1"
                     /translation="QDCAGIYTSLSNGQFSLVVDGNTSGGTLASEQFYFDGRTGGLAS
                     MSTKLGANIPNLSTRVGASLRSRDVDEDGAVEIPVVLGNVSAFSTEQRMQWVSFYDFA
                     AIDEYQNNFEKEAVSPGRQIPSVLPVPTGRGDSSGLNDSSDAAGSDISGAGETKSEPR
                     QTPGPDSQGGAPQEEAAPTNEKVFGLADLSYGYYMRLPLSWKNRVAVERLGTRNWYLA
                     DAASGETLLAVQILGPEEELVSGGVRLGDAREYTQVAAVGRYRIFVRIRPDTGIDEAE
                     ILAGVTVLS"
     gene            965..>1410
                     /locus_tag="ACZPFK_13685"
     CDS             965..>1410
                     /locus_tag="ACZPFK_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1407405.1"
                     /translation="MRRILVVEDEDAIREVIALNLRMAGYDVAEAASAERALELLGAE
                     QDGFDVALLDVMLPGMNGFSLCETIRRDSAEVGIIILSAKTQEKDKINGLSIGADDYI
                     TKPFSVSELLARVEALCRRVTRQATERQGESRLVSGQFVLDQKSRVL"
BASE COUNT          253 a          426 c          505 g          226 t
ORIGIN      
        1 gcaggactgt gccggcattt ataccagcct ctccaacggg cagttcagcc tggtggtgga
       61 cggcaacacc tccggcggca cccttgccag cgagcagttc tattttgacg gccgcaccgg
      121 gggccttgcc agcatgagca caaagctggg ggccaatatc cccaaccttt ccacccgggt
      181 gggggccagc ctgcgcagcc gagacgtgga tgaggacggc gcggtggaga tcccggtggt
      241 gctgggcaat gtgtcggcgt tttccacaga gcagcggatg cagtgggtga gcttttacga
      301 tttcgccgcc atcgacgaat accagaacaa ctttgaaaaa gaggccgtct cgccggggcg
      361 gcagatcccc tccgtgctgc cggtgcccac cggcaggggg gactcgtccg ggctgaacga
      421 cagctcggat gccgccggct cagatatctc aggcgccggc gagacgaaaa gcgagccgcg
      481 gcaaaccccc ggtccggaca gccagggcgg cgccccgcag gaggaggccg cccccaccaa
      541 tgaaaaggtc tttggcctgg cggacctgag ctacggctat tacatgcgcc tgccgctgag
      601 ctggaagaac cgggtcgcgg tggagcggct gggcacccgc aattggtatc tggccgacgc
      661 ggccagcggc gagacgctgc tggcggtgca gatcttgggc cccgaagagg agctggtcag
      721 cggcggcgtc cggctggggg atgcgcggga gtacacccag gtggcggctg tggggcggta
      781 ccgcatcttt gtgcgcatcc gcccggacac cggcatcgac gaggcggaga tcttggccgg
      841 ggtgacggtg ctgagctgac ggcgttcttt tgtagatgcg tccggtcccc gcccttttgg
      901 gacggctggg gagaggcctc cccactgcgg gccggcacga tttccggaaa ggaatgggca
      961 aacgatgaga agaattttgg tcgtggaaga tgaggacgcg atccgcgagg taatcgccct
     1021 caacctgcgc atggcggggt acgacgtcgc cgaggcggcc agcgccgagc gggcgctgga
     1081 gctgctgggg gcggagcagg acggcttcga cgtggcgctg ctggacgtga tgctgcccgg
     1141 catgaacggc ttttccctct gcgagaccat ccgccgggat tccgccgagg tgggcatcat
     1201 catcctgtcg gcaaagacgc aggaaaaaga caagatcaac gggctctcca tcggcgcgga
     1261 cgactacatc accaagccgt tcagcgtctc cgagctattg gcccgggtgg aagcgctctg
     1321 ccgccgcgtg acccgtcagg ccaccgagag gcagggcgag agccgcctgg tgtccggcca
     1381 gtttgtactg gaccagaaga gccgtgtgct
//
LOCUS       DBMDYA010000730         1407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17554_length_1407_cov_0.968606, whole genome shotgun sequence.
ACCESSION   DBMDYA010000730 DBMDYA010000000
VERSION     DBMDYA010000730.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17554_length_1407_cov_0.968606"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="ACZPFK_13690"
     CDS             <1..210
                     /locus_tag="ACZPFK_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407406.1"
                     /translation="PGRPALWAGVPVLWGKVWAKAREGGKRHTGEIRAVFFAKFSKKY
                     PRRMCQSFCSDKQIVTAMLRNFEQR"
     gene            529..>1050
                     /locus_tag="ACZPFK_13695"
     CDS             529..>1050
                     /locus_tag="ACZPFK_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407407.1"
                     /translation="MEAVKAFIESSPLMCFFVIAFIYMIGDMVGTMSKAWVPSVFVVA
                     VLFLIGYWTILPKEVIGTSGLNSTFIGVLCPYILVVHMGTTISVKELAKQWKTIVCCL
                     AGLVGMCALCMLLPVIGVVDRSLVVSGLPPLTGGIVATKIMTEAATNAGLPAAALFAT
                     VMMVVQGFAGYPIT"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1061..>1407
                     /locus_tag="ACZPFK_13700"
     CDS             <1061..>1407
                     /locus_tag="ACZPFK_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407408.1"
                     /translation="AICLKKEGRRLLKDYRSGNVKVTAAAGAVDEANGKMASTEPEKK
                     KLIPSVPEKYASTAFSLAKLALLAVAAFYVGKIQYGTFTISGMVWALIFGIVATELGF
                     LEKDLLHKCGCFN"
BASE COUNT          289 a          410 c          420 g          278 t
ORIGIN      
        1 ccggggcgcc cggcgctctg ggcgggtgtg cccgtcctgt ggggaaaggt gtgggcaaag
       61 gcgcgggagg gcggaaaacg gcatacaggg gaaatccgcg ccgttttttt tgcaaaattc
      121 tctaaaaaat atcctcggcg gatgtgccaa agtttctgct ctgataaaca gattgtaacg
      181 gcaatgttac gaaattttga acaaaggtaa aaaaacagtt gcaattcccg gaaaagctcc
      241 gtataatttc atcaatcccc acccgcagtc cctggtttta tttatatttc gatactctgc
      301 tgcgtcgaag cgcaaaaggg aacgtgcttt tcagcgtggg gatgggaaaa aatcggcttt
      361 tcaccggcct gaccggtgcg caaaaccgga cgtgcgtcgg ccgcgcgcgg cccggttttt
      421 agaagcaaaa acgagcggcg gggaccggac gcgggtctgt ggggcggcga aacaggcgct
      481 cacgggtcag ggttcgatcg ccgcggcccg aaagaggagg aaaacaaagt ggaagcagta
      541 aaagcattta ttgaatccag cccgttgatg tgcttcttcg tgatcgcgtt catctacatg
      601 atcggcgaca tggtcggcac catgagcaag gcgtgggtac cgtccgtctt cgtggtggcg
      661 gtgctgttcc tgatcggcta ctggaccatc ctgcccaaag aggtcatcgg caccagcggc
      721 ctgaactcga cctttatcgg ggtgctgtgc ccctatatcc tggtcgtcca catgggcacc
      781 accatcagcg tcaaagagct ggccaagcag tggaagacca ttgtctgctg cctcgccggc
      841 ctggtgggca tgtgcgcgct ctgcatgctg ctgccggtca tcggcgtggt tgaccgctca
      901 ctggtcgtct ccggcctgcc gcccctcacc ggcggtatcg tggccaccaa gatcatgacc
      961 gaggccgcca ccaatgcggg cctgcccgcc gcggcgctct tcgccaccgt catgatggtg
     1021 gtccagggct tcgccggcta tcccatcacc nnnnnnnnnn gccatctgcc tgaaaaaaga
     1081 gggccgccgc ctgctgaaag actatcgctc cggcaacgtc aaggtgaccg ccgccgcggg
     1141 cgccgtcgat gaggccaacg gcaagatggc cagcaccgag cccgaaaaga aaaagctcat
     1201 ccccagcgtg cccgagaaat atgccagcac cgcgttctcg ctggccaaac tggccctgct
     1261 ggccgtggcc gctttctatg tcggcaagat tcagtacggc acgttcacca tcagcggcat
     1321 ggtctgggcg ctgatcttcg gcatcgtcgc caccgagctg ggcttcctgg agaaagacct
     1381 gctgcacaag tgcggctgct tcaactt
//
LOCUS       DBMDYA010000731         1406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17561_length_1406_cov_1.962452, whole genome shotgun sequence.
ACCESSION   DBMDYA010000731 DBMDYA010000000
VERSION     DBMDYA010000731.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17561_length_1406_cov_1.962452"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(215..1075)
                     /locus_tag="ACZPFK_13705"
     CDS             complement(215..1075)
                     /locus_tag="ACZPFK_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1407409.1"
                     /translation="MSNTRYAMPGQPDSRISARLLYVTTSKYEGDWSSIPHTHYFAEF
                     FYVLSGRGRFQVESEQFEVRADDLIIVNPNIEHTELSLDQNPLEYIVLGVDKMAFDFD
                     GAGAAYSVYNYGAQRDRLLFYLREMLREIEGHEKGYEVVCQNLLEVLVLNMMRSAHFS
                     MAPVDNQKTSKECSAVKRYIDSNFGDDISLDTLAKRTHLNKYYLVHAFTRANGISPIN
                     YLIERRIEGSKQLLESTNHNISQIARIVGFSSQSYFSQSFKRLTGMTPAQYRKQARQA
                     EAAPQEKKEA"
BASE COUNT          299 a          420 c          361 g          326 t
ORIGIN      
        1 gacgatgaaa aaagagcgca gcggcataag gcgcgcgctc ggcgcccatc ctcccggtga
       61 ggggccgcac agccgcagaa atccgcctgc cccggcaggc cccgcttcgg cccgcgccgg
      121 accaaagggg cgcgccccga gccgccgggc cacttctccg ggccgaaaag ccattttccc
      181 gagccgcggc accgtttttc ggggcgcccg cacctcaggc ctcttttttc tcctggggcg
      241 ccgcttcagc ctgccgcgcc tgcttgcggt actgcgccgg ggtcatgccg gtcagccgct
      301 tgaagctctg cgaaaaatag ctctgggagg aaaagcccac gatccgcgcg atctgcgaga
      361 tgttgtggtt ggtgctctcc agcagctgct tcgacccctc gatgcgccgc tcgatcaggt
      421 agttgatggg cgagatgccg ttggcccgcg taaaggcgtg caccagatag tatttgttga
      481 gatgggtgcg ctttgccagc gtgtccagcg agatgtcgtc gccgaaattc gagtcgatgt
      541 accgcttgac ggcgctgcac tccttgctgg ttttctggtt gtccaccggc gccatggaaa
      601 aatgcgccga gcgcatcatg ttcagcacca gcacctccag caggttctgg cagaccacct
      661 catagccctt ttcatggccc tcgatctccc gcagcatctc ccgcaggtaa aacagcagcc
      721 ggtcgcgctg cgccccgtaa ttgtagacgc tgtaggccgc gcccgccccg tcaaagtcga
      781 acgccatctt gtccacaccc agcacaatat actccagcgg gttctggtcc aggctgagct
      841 cggtgtgctc gatattgggg ttgacgatga tcaggtcgtc tgcccgcacc tcaaactgct
      901 cgctctccac ctgaaagcgg ccgcgcccgg agaggacgta aaaaaattcg gcaaaatagt
      961 gggtgtgcgg gatactgctc cagtccccct catattttga cgtcgtcaca tacagcagcc
     1021 gcgcgctgat gcggctatcc ggctggccgg gcatggcata tcgcgtattg ctcaaaagat
     1081 cagccccatt tttagtaata ttgctaaaca tctgaaagaa gcgggaattt ccagcagttt
     1141 ccattattat aaaccgtttt ttcatcagat acaagtatac aatataaact cattttacct
     1201 ggtattttat gtgcaaaaca accattaatt ttttacatcc tttccatttt aaaccatttt
     1261 acctaaaaac ttttttcttc tccctctgtt tttgtgggat tattttaaag caatatttta
     1321 aaaattcaaa gcaagaaaga aatttgaatc ccgctttact ggaactatac tgaaagtgcg
     1381 atcggggagc gcggctcccc accacc
//
LOCUS       DBMDYA010000732         1406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17562_length_1406_cov_1.829119, whole genome shotgun sequence.
ACCESSION   DBMDYA010000732 DBMDYA010000000
VERSION     DBMDYA010000732.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17562_length_1406_cov_1.829119"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            142..828
                     /locus_tag="ACZPFK_13710"
     CDS             142..828
                     /locus_tag="ACZPFK_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1407410.1"
                     /translation="MFGTFLSFIAGHMLYFWLHLESSSFPRPLSSKEEAECLAALGRG
                     DMEARDRLIRHNLRLVAHVVKKYYPGSADTDDLISIGTIGLIKAVASFDSTKKARFST
                     YAAKCIENEILMYFRSDKKNRGTLYINEPLEADGGEGALTLNDVLADSFFVDEDYEQK
                     ADAERLRGYVDTILDGRERQIVRLRYGFAGGDPLSQQQVADILGISRSYVSRLEKKAV
                     EQLKTAMERE"
     gene            1198..>1406
                     /locus_tag="ACZPFK_13715"
     CDS             1198..>1406
                     /locus_tag="ACZPFK_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407411.1"
                     /translation="MKPDGCLQSGAIHPSPLARAAWIKIALSASGREGTGVKTICFPV
                     LGESKKKKGQDEKIAPVLFLSNFYL"
BASE COUNT          323 a          353 c          427 g          303 t
ORIGIN      
        1 aggtgaagca ctgcatggta cacgtcaacc ccgagctggc ctgtactggg caggaagaaa
       61 aaacataacc tgtttccccg ccccatatcc tgtatggatg ggggcgggcg cagcccccga
      121 ttgaaccggg ggtgtttttt tatgttcggg acctttctca gctttatcgc cggccacatg
      181 ctctatttct ggctgcatct ggagagcagc agctttccgc gccccctgtc gtccaaggag
      241 gaggcggagt gcctggccgc gctggggcgc ggggatatgg aggcccggga caggctgatc
      301 cggcacaacc tgcgcttggt cgcgcatgtg gtgaaaaagt attacccggg cagcgccgat
      361 accgatgacc tgatcagtat cggcaccatc ggcctcatca aggcggtggc gagctttgac
      421 agcaccaaaa aagcgcgttt ttccacctat gcggcaaaat gcatcgaaaa tgaaatcttg
      481 atgtacttcc gctcggataa aaagaaccgc ggcaccctgt acatcaacga gcccctggag
      541 gccgacggcg gcgagggggc gctgaccctg aacgacgtgc tggcggacag cttttttgtg
      601 gatgaggact atgaacaaaa ggccgacgcg gagcggctgc gcggctatgt ggacaccatt
      661 ctggacggcc gcgagcggca gatcgtgcgg ctgcggtacg gctttgcggg gggcgatccc
      721 ctgagccagc agcaggtggc ggatatcctg ggcatctccc gcagttatgt gagcaggctg
      781 gagaaaaagg cggtggagca gctgaaaacc gcgatggaga gagaataagc atcacgcagg
      841 gcggaggaaa gcggcctttc ctccgccctg gttttcgcgg tgtgattttt ttgctgcggt
      901 aacgggggat atttcatttt ttcgcatact ccgctataat aagtaaagga actgccgctt
      961 tgccgcattt ctgcagaggt tgaaacggcg cggcggccgg gtgcgaaagg caagctctca
     1021 tgccagtgca gcaaggttcg caccgaaaaa agaggttgtt gttctgattt ttttaccaca
     1081 agtaagacaa aagagggagg aaaatcaaga aacagacgtt ttgtttgcgg ttttgcataa
     1141 gtttaaggct cgtttattgt gcatcttcgc agtcgccgct cgcaagagcg gcgtggattg
     1201 aaaccagacg gttgcctaca atcaggagcg atacacccgt cgccgctcgc aagagcggcg
     1261 tggattaaaa ttgcgctctc ggcttctgga cgcgagggca ccggggtcaa aactatttgt
     1321 tttcccgtgt taggcgaatc taaaaagaaa aaaggacagg acgaaaaaat cgctcctgtc
     1381 ctttttctgt ccaatttcta tctgaa
//
LOCUS       DBMDYA010000733         1405 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17585_length_1405_cov_1.184816, whole genome shotgun sequence.
ACCESSION   DBMDYA010000733 DBMDYA010000000
VERSION     DBMDYA010000733.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17585_length_1405_cov_1.184816"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1405
                     /locus_tag="ACZPFK_13720"
     CDS             <1..>1405
                     /locus_tag="ACZPFK_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1407412.1"
                     /translation="LGVVLLNAVVGMVQEGKAQKALDALKKMTSPTATVRRSGIVKEI
                     PAASLVRGDVVLLDAGRVVPADLRLISTASLKIDESALTGESVPAEKDAAFLAEKDAP
                     LGDRHNMAYSSTSVTYGRGEGVVVGTAADTEIGKIAAMLQDEDELTPLQKSLAVLGKL
                     LGGIAVLLCAALFVIALVQGRDVVEMLLTAISLAVAAVPEGLPAVVTIVLAMGVQRMV
                     KVNSIVRRLPAVETLGAVSVVCSDKTGTLTQNKMTVTKIYVDGALRDPQSLNAESDRL
                     FLKGFVLCNDATAAGENRVGDPTELALLDMGAAVGVTREGLEEACPRVNEQAFDSGRK
                     MMTTVHRSGEETAAYTKGAVDQLLKHCTGIVENGAARPITAQDKENILSAASAMSRQA
                     LRVLAMAVKYGDESAEEEDLQFVGLVGMIDPARPEAKDAVESFKQAGITTIMITGDHR
                     DTALAIARELGIAEAES"
BASE COUNT          258 a          459 c          496 g          192 t
ORIGIN      
        1 ttttgggcgt ggtgctgctc aatgcggtgg tcggcatggt gcaggagggc aaggcccaaa
       61 aggcgctgga cgccctgaaa aagatgacca gccccaccgc caccgtgcgc cgcagcggca
      121 tcgtcaagga gatccccgcc gccagcctgg tacggggcga cgtggtgctg ttggacgcgg
      181 gccgggtggt gcccgccgac ctgcggctca tctccaccgc cagcctgaag atcgacgaga
      241 gcgcgctcac cggcgagagt gtgcccgcgg aaaaggacgc tgcttttctt gcggaaaaag
      301 acgcgccgct gggcgaccgc cacaacatgg cctacagcag caccagcgtg acctacggcc
      361 gcggcgaggg cgtggtggtg ggcaccgccg ccgacaccga gatcggcaag atcgccgcca
      421 tgctgcagga tgaagacgag ctgaccccgc tgcagaaaag cctggccgtg ctgggcaagc
      481 tgctgggcgg catcgccgtc ctgctctgcg cagccctctt cgtcatcgcc ctggtgcagg
      541 gccgcgatgt ggtggagatg ctgctcaccg ccatcagcct tgccgtcgcc gccgtgcccg
      601 aggggctgcc cgccgtggtc accatcgtgc tggccatggg ggtgcagcgg atggtcaagg
      661 tcaacagcat cgtgcgccgc ctgcccgccg tggagaccct gggcgcggtg agcgtggtct
      721 gcagcgacaa gaccggcacc ctgacccaga acaagatgac ggtcaccaag atctatgtgg
      781 acggcgccct gcgcgacccg caaagcctga acgccgagag cgaccggctg tttttgaaag
      841 ggttcgtgct ctgcaacgac gccaccgcgg cgggtgaaaa ccgggtgggc gaccccaccg
      901 agctggctct gctggacatg ggcgccgccg tgggcgtcac ccgcgaaggg ctggaagagg
      961 cctgcccgcg cgtcaatgag caggcgtttg actccggccg caagatgatg accactgtcc
     1021 accgcagcgg cgaggagacg gcggcctaca ccaagggcgc cgtggaccag ctgctcaagc
     1081 actgcaccgg catcgtggaa aacggcgccg cgcggccgat cacggcgcag gacaaagaaa
     1141 acatcctgtc cgccgcctcg gccatgtcca ggcaggcgct gcgtgtgttg gccatggcgg
     1201 tcaagtacgg ggatgagtcg gcggaggaag aggacctgca attcgtgggc ctggtgggca
     1261 tgatcgaccc cgcgcggccc gaggccaagg acgcggtgga gagcttcaag caggccggca
     1321 tcaccaccat catgatcacc ggggaccacc gcgacaccgc gctggccatc gccagggagc
     1381 tgggcatcgc cgaggcggag agcca
//
LOCUS       DBMDYA010000734         1400 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17653_length_1400_cov_2.032333, whole genome shotgun sequence.
ACCESSION   DBMDYA010000734 DBMDYA010000000
VERSION     DBMDYA010000734.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17653_length_1400_cov_2.032333"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..596)
                     /locus_tag="ACZPFK_13725"
     CDS             complement(105..596)
                     /locus_tag="ACZPFK_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407413.1"
                     /translation="MKVKTNRLIASGLFSVLFIGELYLLLSVVSQFGTNSMAIGHLLV
                     IGGVLLLPVFAVLIANPCFDRPAVKGAVLYSGVLYACACALLFSAGSMLYATGLQSET
                     QTAGYYVVAAKVVLVVVALVIAAFEPKAKKEADSDMEELSADCAEETATETSDDEAGK
                     MGL"
     gene            complement(701..>1400)
                     /locus_tag="ACZPFK_13730"
     CDS             complement(701..>1400)
                     /locus_tag="ACZPFK_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR1407414.1"
                     /translation="SAESDEGYLIMVTKNGVIKRTPLASYRNIRKSGLIAISMDEGDS
                     LAWTRITSGSDELIVATHNGMAIHFTEEDVRVVGRVARGVKAITLDEGDYVVGMATIR
                     EGGELLTVTEQGLGRRTPISEYRLQYRGGKGIKNYSGKGGAVAGVKVVDPQDDIILIS
                     LEGIIIRMHVEDINVQSRYAGGVRVMRLGETDRVVTVARTPREEEEPDAAEDDSMEQA
                     PDTTAAPAGEESGD"
BASE COUNT          244 a          429 c          439 g          288 t
ORIGIN      
        1 gcaataaaac ataattgatc ttcatggccg cggcccaggg atgggccaca tggcaaaaag
       61 aatagccgca tctgcggcta ttctttgtca gaagcaagtt tcttttacag gcccatcttt
      121 cccgcttcgt cgtcgctggt ctcggtggcg gtctcctcgg cgcagtcggc ggacagctcc
      181 tccatgtcac tgtccgcttc ctttttcgcc ttgggctcaa aggcggcgat caccaaagcc
      241 accacgacca gcaccacctt ggcggcgacc acataataac cggcggtctg ggtctcagac
      301 tgcagaccgg tggcataaag catggagccg gcgctgaaca gcagggcgca ggcgcaggcg
      361 tagaggacgc cgctgtagag cacggcgccc ttgaccgccg ggcggtcaaa gcaggggttg
      421 gcgatgagca cggcgaacac cggcagcagc agcacgccgc cgatcaccag cagatggccg
      481 atcgccatac tgttggtgcc gaactggctg acgaccgaga gtaaaagata cagctcgccg
      541 atgaaaagga cgctgaacag cccggaggcg atgagacggt tggttttgac tttcatggat
      601 tggttgctcc cttatctttt tgtccgggct ccccggcagt gaatcataac gtctattata
      661 aattgcctat gttaaggttg tgtgaaaaga ggagaggggc tcagtctccg ctttcctccc
      721 cggcaggggc ggcagtggtg tcgggcgcct gctccatcga gtcgtcctct gcggcgtcgg
      781 gctcctcttc ctcgcggggg gtgcgggcca cggtgaccac gcggtcggtt tcgcccagac
      841 gcatcacccg cacgccgccg gcataccggc tctgcacgtt gatatcctcc acatgcatgc
      901 ggatgatgat gccctccagc gagatgagga tgatgtcgtc ctgcgggtcc accaccttga
      961 cgccggcgac cgcgccgccc ttgccgctgt agtttttgat gcccttgccg ccgcggtatt
     1021 gcaggcggta ttcgctgatg ggggtgcggc ggcccaggcc ctgctcggtg acggtgagca
     1081 gctcgccgcc ctcgcggatg gtggccatgc cgaccacata gtcgccctca tccagcgtga
     1141 tggccttgac gccccgggcg acgcggccca cgacgcgcac atcctcttcg gtaaagtgaa
     1201 tggccatgcc gttgtgtgtg gccacgatca gctcgtcgct gccgctggtg atgcgggtcc
     1261 atgccagact gtcgccctcg tccatcgaga tggcgatcag gccgctcttg cggatgttgc
     1321 ggtagctggc cagcggggtg cgtttgatga cgccgttttt ggtgaccatg atgagatagc
     1381 cctcgtcgct ctctgccgag
//
LOCUS       DBMDYA010000735         1389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17806_length_1389_cov_1.420031, whole genome shotgun sequence.
ACCESSION   DBMDYA010000735 DBMDYA010000000
VERSION     DBMDYA010000735.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17806_length_1389_cov_1.420031"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="ACZPFK_13735"
     CDS             <1..444
                     /locus_tag="ACZPFK_13735"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1407415.1"
                     /translation="YTVKKKVSEELLFCAFVPDFELLTEKARAALPQQVPHRDAVYNA
                     ARAALMAAAFCEGRPDLFAVASGDKLHQPYRLPLIPGARRIFEIAMQLGARAVFISGA
                     GPTIMAVCDAGDDSFVPRAILALEEDAATRRFAVCRYQAQNASMF"
     gene            530..>1389
                     /locus_tag="ACZPFK_13740"
     CDS             530..>1389
                     /locus_tag="ACZPFK_13740"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1407416.1"
                     /translation="MALIVQKFGGSSVADRDHLFNVARIVADTHNAGNDVIVVVSAQG
                     DTTDDLIAKAGEITCDPSGREMDMLLAAGEQISCSLLCMALSELGCRAISLLGWQAGF
                     ITDRNHQRARIRKVDTERLECEIGKNQVVVVAGFQGLNRADDLTTLGRGGSDTSAVAI
                     AAAMKADLCQIYTDVEGVYTADPRVVGKAKKLAEISFDEMLELATLGAQVLNNRSVEL
                     AKKYAVRLEVLSSLNPVPGTIVKEVAAMEGMMIKGVAKDENVSAISVLSVHDVPGIAF
                     KVFSLLAQRK"
BASE COUNT          249 a          416 c          494 g          230 t
ORIGIN      
        1 tatacggtca aaaaaaaggt cagtgaagag ctgctgttct gcgcctttgt gccggatttt
       61 gagctgctca ccgaaaaagc gcgggccgcc ctgccgcagc aggtacccca ccgggacgcg
      121 gtctacaacg ccgcgcgcgc ggccctgatg gccgccgctt tctgcgaggg caggcccgac
      181 ctgtttgcgg tggcgtcggg ggataagctg catcagccgt atcggctgcc gctcatcccg
      241 ggcgcgcggc gcatctttga aattgccatg cagctgggcg cgcgggcggt gttcatcagc
      301 ggcgcggggc ccaccatcat ggcggtctgc gacgcgggcg acgactcgtt tgtgccgcgg
      361 gcgatcctgg cgttggagga ggacgccgcc acccgccgct ttgcggtgtg ccggtatcag
      421 gcgcagaacg ccagcatgtt ctagcgcttc tccccaaaag gcgggcggac gcgtcgccct
      481 tgggcctgca ggggaaacag ttcgagaggg gatttgaggg aggaagagta tggcactgat
      541 cgtacagaag ttcggcggtt cctcggtcgc ggaccgggac cacctgttca acgtggcccg
      601 catcgtcgcg gacacccaca acgcgggcaa cgacgtgatc gtggtggtct cggcccaggg
      661 ggacaccacc gacgacctga tcgccaaggc gggggagatc acctgcgacc cgtccgggcg
      721 cgagatggat atgctgctgg ccgccggcga gcagatctcc tgctcgctgc tgtgcatggc
      781 cctgtcggag ctgggctgcc gggccatttc gctgctgggc tggcaggcgg gcttcatcac
      841 cgaccgcaac caccagcggg cgcgcatccg caaggtggac accgagcggc tggagtgcga
      901 gatcggcaaa aaccaggtgg tggtggtggc gggctttcag gggctcaacc gggccgacga
      961 cctcaccacg ctggggcgcg gcggcagcga caccagcgcc gtggccatcg cggcggcgat
     1021 gaaagccgac ctttgccaga tttacaccga cgtggagggc gtgtacaccg ccgatccgcg
     1081 ggtggtgggc aaggcgaaaa agctcgccga gatcagcttt gacgagatgt tggagctggc
     1141 caccctgggg gcccaggtgc tcaacaaccg ctcggtggag ctggcgaaaa aatatgccgt
     1201 gcggctggag gtgctctcaa gcttaaaccc cgtgcccggc acaattgtga aggaggtcgc
     1261 agctatggaa ggtatgatga tcaagggggt cgcaaaagac gagaacgtct cggccatttc
     1321 ggtgctcagc gtgcacgacg tgccgggcat cgcgttcaag gtgttcagcc tgctggcgca
     1381 gcgcaagat
//
LOCUS       DBMDYA010000736         1387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17842_length_1387_cov_1.646190, whole genome shotgun sequence.
ACCESSION   DBMDYA010000736 DBMDYA010000000
VERSION     DBMDYA010000736.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17842_length_1387_cov_1.646190"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            560..1273
                     /locus_tag="ACZPFK_13745"
     CDS             560..1273
                     /locus_tag="ACZPFK_13745"
                     /inference="COORDINATES: protein motif:HMM:NF021093.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR1407417.1"
                     /translation="MKRFKTALGLGLLLAIVLTCSFSSFSAVCAEIRRDTLRLHILAN
                     SDTEADQQLKLAVRDAILAETGGLFGSAGSKEAAERSVDANLDVIRSIAETVIREQGY
                     DYPVRVTRTNLYFATTRYPDTGLTMPAGRYDAVRVEIGKAQGKNWFCVLFPPLCVPAA
                     TADAAQEAAYTEEERAVTGGGYEVRFAVLEWLEQARESLARAVKKPDDAVAADSAPRQ
                     TPAPTEGASADGAPQGGRP"
BASE COUNT          273 a          404 c          464 g          246 t
ORIGIN      
        1 cgtatcttta aaaaggccgg caatccgcag ggattgccga ccttttttgc acgatgtctt
       61 tttcaaaagc gccggtgtaa agccgccgct cacccacggg aggcaaaatg gcgcggcaaa
      121 gctagaaaat ttcatccaga ttgcctgtgg cggcgtttgc cggcctgcgc aggctttccg
      181 ctgtgcggaa ccgcaaaatc atcagtggcc gcggggtgaa tttgacggcc ctctgcgcgc
      241 aactatcccc ctctcggggg aaaagcagag ctttcttgcg cagacgcgct cttgaggctg
      301 ctttttgcgc cacctccttt ctcagaacaa aaatgcggat ctgcggccgc cgtgcggcct
      361 cttttgcgcg ggaaaaggct gggctggggg cagagggcat agctgtttta aggaggcgag
      421 cctcccgtct gtacttgccg gaacaaaaaa tgaaaccggc ggaatatttt accagaaccg
      481 tgccgatact ctgggcagag caagggaggc ggcggatgcc ggacggccgc cgcgcccgca
      541 cagagaaagg cgtgtttttt tgaaacggtt caaaacggcc ctgggcctcg gcctgttgct
      601 ggccatcgta cttacctgtt ctttttccag cttttcagcg gtctgtgcgg agatccgccg
      661 ggacaccctg cggctgcaca ttttggcaaa ctccgacacg gaagcggacc agcagctcaa
      721 gctggcggtg cgggacgcga tcctggccga gacgggcggc ctttttggct ccgccggcag
      781 caaagaggcg gcggagcgca gcgtggacgc gaatctggat gtcatccgca gcatcgccga
      841 gacggtgatc cgggagcagg gctatgacta tccggtgcgg gtcacccgca ccaatctgta
      901 ctttgccacc acccgctatc ccgataccgg gctgaccatg ccggcgggcc gctacgatgc
      961 ggtgcgggtg gagatcggta aagcgcaggg aaaaaactgg ttctgcgtgc tctttccgcc
     1021 gctctgcgtg ccggcggcca cggcggacgc cgcgcaagag gcggcttata ccgaggagga
     1081 acgggcggtc accggcggcg gctacgaggt gcgctttgcg gtgctggaat ggctggagca
     1141 ggcgcgggag agcctggcgc gggcggtgaa aaagccggac gacgcagtgg ctgcggacag
     1201 tgcgccgcgg cagacccccg ccccgacaga gggggcaagc gcggacggcg ccccgcaggg
     1261 cggacgccct tagacacaca aaaaagaaaa gagcaacagg cggccgggaa tgcgctcccc
     1321 gtccgcctgt tgctctttgg ccgtcgctgc gaaaaggccg aaagatgttt tttgagacgg
     1381 ggaaagc
//
LOCUS       DBMDYA010000737         1386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17857_length_1386_cov_1.324514, whole genome shotgun sequence.
ACCESSION   DBMDYA010000737 DBMDYA010000000
VERSION     DBMDYA010000737.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17857_length_1386_cov_1.324514"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="ACZPFK_13750"
     CDS             <1..191
                     /locus_tag="ACZPFK_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HIR1407418.1"
                     /translation="SSCELDPGRYDVFVTKFGYIFPELKELGKSFIMSNTPGESYQMI
                     TEFDYKKIRRPIYPLDDI"
     gene            733..>1386
                     /locus_tag="ACZPFK_13755"
     CDS             733..>1386
                     /locus_tag="ACZPFK_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1407419.1"
                     /translation="MGKAITIRDIAKEAKVSPTTVSRYLNGTGYVDEQTASRICEVVE
                     RFHYRPNRIAQSLKTKETKNLVLVVPDIQNPFYSKMANEMQRFLFEEGYTVTLFDSGG
                     EFSMEMKYLGIADAIAADGILFASFSSHQTILRELQRSGVPVVMINSYDACPFDSVHG
                     VRGQSTYLSTKHLIALGHTDIGFAGGNTGTSIEVSRRAGYLRAMQEAGLPVPGEYVFE
                     "
BASE COUNT          297 a          388 c          427 g          274 t
ORIGIN      
        1 tctcctcctg tgagctggac cccggccgct acgacgtctt tgtgaccaag ttcggctata
       61 tcttccccga gctcaaagag ctgggcaaaa gctttatcat gtccaacact ccgggggaga
      121 gctatcagat gatcaccgag ttcgactaca aaaagatccg gcggcccatc tatccgctgg
      181 acgatatcta aagcgggacc cggggacggg cggctgccct ggcatgcgaa agcgcgcttc
      241 tttttcagga agcgcgcttt cgcttttttt gcccctggcc gggcggggca agaggcggga
      301 gaaatataaa aaagttgcgc ttctgcactg cggcgcgatc aaggaaaggg cgcggcggga
      361 gcgcgccgcc gccggcccgg cgggggagag gtggagagga cgaaggcggg cggtaccggg
      421 ggctgttttg gccgccgggc gcccggagcg ggggactgga aagcgggggc ccgcgcttgg
      481 tcccctcgct gtgagcggcg gggaacggct tttttgggtt ccgcgaaaaa ggaagaatca
      541 tgaagaatgt gaaaccgttt cctctaaatg cgaaagctat tttttgtgtc caatacacat
      601 acaaccaccc ctaatcttgt gcatgattac agaaaatgat gacaacaaag gaaaagttgt
      661 tgacatttcg cgggtgcgaa aatataattc aggttatggt aacggttaca ccttgcaaag
      721 gggaggggaa caatgggaaa ggccatcacg atccgtgata ttgccaaaga agcaaaggtc
      781 tcccccacca ctgtctcccg ttatctgaac ggcaccggtt atgtggacga gcagaccgcc
      841 agccgtatct gcgaggtggt ggagcggttc cattaccgcc ccaaccgcat cgcccagagc
      901 ctcaagacca aggaaactaa aaatctggtg ctggtcgtgc cggacatcca gaatcctttt
      961 tattccaaga tggccaacga gatgcagcgc tttctctttg aggagggcta caccgtcacc
     1021 ctgttcgatt cgggcgggga gttctcgatg gagatgaaat atctcggcat cgcggacgcg
     1081 attgcggcgg acggcatcct gtttgcctct ttttcctctc accagaccat cctgcgggag
     1141 ctgcagcggt cgggcgtgcc cgtggtgatg atcaactcct acgacgcctg cccctttgac
     1201 tcggtccacg gcgtgcgcgg gcagagcacc tacctgtcca ccaagcacct gatcgcgctg
     1261 ggccacaccg acatcggctt tgccggcggc aacaccggca cctccatcga ggtcagccgc
     1321 cgggcgggct acctgagggc catgcaggag gcggggctgc ccgtgcccgg cgagtatgtg
     1381 ttcgag
//
LOCUS       DBMDYA010000738         1381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_17935_length_1381_cov_1.346094, whole genome shotgun sequence.
ACCESSION   DBMDYA010000738 DBMDYA010000000
VERSION     DBMDYA010000738.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_1381_cov_1.346094"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /gene="xseA"
                     /locus_tag="ACZPFK_13760"
     CDS             complement(<1..>1079)
                     /gene="xseA"
                     /locus_tag="ACZPFK_13760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1407420.1"
                     /translation="DEACSVKAVMFRGNAARLSFEPENGMSVVVRCRVSLYERDGAFQ
                     VYVEDLFPDGLGAAQMAFEQLKARLAKEGLFAPEIKKALPPFPRRIGLVTSKSGAALH
                     DILSVAQRRWPAAHFLLAHAGVQGREAEDELAGAVRRLDVGGQVDVIIVARGGGSRED
                     LWVFNGEKLARTVYACRTPVVSAVGHEIDYTILDFVADLRAATPTAAAELVLPNGPAL
                     RMQLAQLREKARALVLRRLQMCYNEYYALSTSSAYARLSRRVERSQQQLDVAQGALRG
                     AVRRAAAQRAEQLSQAAALLDSLSPYRTLSRGYCAALKAGRAVRLREELAEGDRLTLL
                     FSGFGARCTVVGITEGSLPAEAKTE"
     assembly_gap    1080..1089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1090..1327)
                     /locus_tag="ACZPFK_13765"
     CDS             complement(<1090..1327)
                     /locus_tag="ACZPFK_13765"
                     /inference="COORDINATES: protein motif:HMM:NF025118.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407421.1"
                     /translation="MSRPGCPVCASCRGRFHPTTRPGWRFWPRGAWRDDMANIITVTA
                     LNRYVKSLLESDAVLTDVAIRGEIQNFVHHRSGHF"
BASE COUNT          234 a          504 c          432 g          201 t
ORIGIN      
        1 gctctgtttt tgcttcagcc ggcaagctcc cttccgtgat cccaaccacc gtgcagcgcg
       61 ccccaaagcc gctgaacagc agggtgagcc ggtcgccctc ggccagctcc tccctgaggc
      121 gcaccgcccg cccggccttg agggcggcgc agtagccgcg ggacaaggtg cggtacgggc
      181 tcagcgagtc cagcagcgcc gccgcctgcg agagctgctc cgccctctgg gccgccgccc
      241 ggcggacagc gccccgcagc gccccctgcg cgacgtcgag ctgctgctgc gaccgttcca
      301 cccgccgcga caggcgcgcg taggcggaag aggtcgacaa tgcgtaatat tcattatagc
      361 acatctgcag ccgccgcaaa accagggcac gggctttttc ccgcagctgg gccagctgca
      421 tccgcagcgc cgggccgttc ggcaatacca gctcggccgc cgcagtgggg gtcgctgcgc
      481 gcaggtcggc gacaaaatcc aggatcgtgt agtcgatctc gtgccccacc gcgctgacca
      541 ccggcgtgcg gcaggcatag acggtgcggg ccagcttttc gccgttgaag acccagaggt
      601 cctcgcggga gccgccgccg cgggcgacga tgatcacgtc cacctgcccg ccgacgtcca
      661 ggcggcgcac cgccccggca agctcgtcct ccgcctccct gccctgcacg cccgcatggg
      721 ccaaaagaaa gtgggcggcg ggccagcggc gctgcgccac cgagaggata tcgtgcaggg
      781 cggccccgct tttggaggtg acaaggccga tcctgcgcgg aaagggcggc agcgcttttt
      841 tgatctccgg ggcgaaaagc ccctcctttg ccagccgcgc cttgagctgc tcaaacgcca
      901 tctgcgcggc ccccagcccg tcggggaaca ggtcctccac atagacctga aaggcgccgt
      961 cccgctcgta gaggctgacg cggcagcgca cgacaacgct catgccgttt tccggctcaa
     1021 agctcagccg ggcggcgttg ccgcggaaca tgaccgcctt gaccgaacag gcctcgtccn
     1081 nnnnnnnnna aaagtgcccg ctgcggtggt gcacaaagtt ctggatctca ccgcggatcg
     1141 ccacatcggt gagcaccgca tcgctctcca gcagagattt gacatatcga ttgagcgccg
     1201 tgaccgtgat gatgttagcc atgtcgtccc tccacgctcc gcgcggccaa aatcgccacc
     1261 ccggccgcgt tgtcggatga aaacgccccc ggcacgaagc gcacaccggg cacccgggcc
     1321 gtgacatagt ctcggataag gccgctgctc atcacgccgc ccgcacacac gcagggcagg
     1381 c
//
LOCUS       DBMDYA010000739         1377 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18000_length_1377_cov_1.665361, whole genome shotgun sequence.
ACCESSION   DBMDYA010000739 DBMDYA010000000
VERSION     DBMDYA010000739.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18000_length_1377_cov_1.665361"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="ACZPFK_13770"
     CDS             complement(<1..567)
                     /locus_tag="ACZPFK_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1407422.1"
                     /translation="MKNTIKLGVIGSGWMADMFCAEGKNVKNVNIAAVFSRNPQRAAA
                     FAQKHGIPAFTADLDELLAGDLDAVYIAATNDRHCALALRALRSRKAVLLEKPFALNS
                     DETLLIIKEAAARGVLVMEAMWLRFFPSVLAMKRLADDGMIGNLRSVTAPCSLLLDRQ
                     KNARVFDPRLGGGVLADIGVYALSFVQML"
     gene            796..>1377
                     /locus_tag="ACZPFK_13775"
     CDS             796..>1377
                     /locus_tag="ACZPFK_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1407423.1"
                     /translation="MDKKVTISDIARDAGVSLATVSRVLNQKGAVRESTGSRVLASAQ
                     KLGYEMPTESEPRSAEGGLLLFCVPSIGNHFYNEVFDGAQTSAQRLGYQFLLYEGHIN
                     RSTLPALQSLIRHTHAQGVVTVNHIAAEVLVKLESLVPVIQCCEFNEALDRSYVSIDD
                     VKASSDAVEYLYSLGRRKIAFLNGPGQYKYARHR"
BASE COUNT          283 a          402 c          422 g          270 t
ORIGIN      
        1 cagcatctgc acaaagctga gcgcgtagac cccgatgtcc gccagcaccc cgccgcccag
       61 ccgcgggtca aagacgcggg cgttcttttg ccggtccagc agcaggctgc agggcgccgt
      121 gaccgagcgc aggttgccga tcatgccgtc gtcggccagc cgcttcatgg ccagcacgct
      181 ggggaaaaag cgcagccaca tggcctccat caccagcacc ccgcgcgcgg ccgcctcttt
      241 tataatgagc agcgtctcgt cgctgttcag ggcaaagggc ttttccagca gcaccgcctt
      301 gcggctgcgc agggccctca gggccagcgc gcagtggcgg tcgttggtgg ccgcgatgta
      361 gacggcgtcc aggtcgcccg ccagcagctc gtccaggtcg gcggtgaaag cggggatgcc
      421 gtgcttttgc gcaaaggccg ccgcgcgctg cgggttgcgg gagaaaacgg cggcgatatt
      481 tacatttttt acatttttgc cctcggcgca gaacatatcc gccatccaac cgctgccgat
      541 taccccgagt ttgattgtat ttttcataat actcatccgg tttttcatta ttttctgaaa
      601 atattgtaac atttgcagtt tgcattttca atagttttct ctcttccgcc agggaaaacg
      661 ttgtaataga caacgaaatg agggctttga gaaacaaagt tacgggggaa actgcgaaaa
      721 taaagaaaat tttctgaaaa ctatttacat tttcagcaaa cggcgttatg atgaggacaa
      781 gaggagtgga agcaaatgga taaaaaagtg acgatctcgg atatcgccag ggacgcgggg
      841 gtgtcgctgg ccaccgtctc gcgggtgctc aaccagaaag gggcggtgcg cgagagcacc
      901 ggcagccgcg tgctcgcctc ggcgcagaag ctgggctatg agatgccgac ggaaagcgag
      961 ccccgatccg ccgagggcgg gctgctgctg ttctgcgtgc cgtcgatcgg caaccatttc
     1021 tataatgagg tgttcgacgg cgcccagacc tccgcccagc ggctggggta tcagtttttg
     1081 ctgtacgagg ggcacatcaa ccgcagcacc ctgccggcgc tgcagtcgct gatccggcac
     1141 acccacgccc agggcgtcgt cacggtgaac cacatcgcgg ccgaggtgct cgtcaagctg
     1201 gaaagcctgg tgccggtgat ccagtgctgc gagttcaacg aggcgctgga ccgctcgtat
     1261 gtgtccatcg acgacgtgaa agcgagctcg gacgcggtgg agtacctgta ctcgctgggg
     1321 cggcgcaaga tcgcctttct caacgggccg ggccagtaca agtacgcccg ccaccgc
//
LOCUS       DBMDYA010000740         1368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18128_length_1368_cov_1.403315, whole genome shotgun sequence.
ACCESSION   DBMDYA010000740 DBMDYA010000000
VERSION     DBMDYA010000740.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18128_length_1368_cov_1.403315"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(480..620)
                     /locus_tag="ACZPFK_13780"
     CDS             complement(480..620)
                     /locus_tag="ACZPFK_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407424.1"
                     /translation="MKKVFAAADGLTRRRRNESLPALKRPAEKFSPPVFFHCCAIKNN
                     LS"
     gene            complement(617..1348)
                     /locus_tag="ACZPFK_13785"
     CDS             complement(617..1348)
                     /locus_tag="ACZPFK_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407425.1"
                     /translation="MRMLTFSSRTAKEILRDPLTAAFGAGFPLVLLLLLSAIQANIPV
                     SLFEISHLAPGIAVFSLSFLTLFSATLISRDRTTALLERLYTTPLTAADYIGGYLLPL
                     LPLALGQSAVCYAGALFLGLKARVEILWSVLWTLPVSLFFIALGLLCGSLFNDKQVGG
                     ICGALLTNVAAWLSGTWFDLELVGGGFKKVAYALPFVHAVELQRAALSGRTADILPHL
                     WWVLGYGVLMAAVAIAVFTHKMRRR"
BASE COUNT          293 a          426 c          375 g          274 t
ORIGIN      
        1 tccgcggggt aaaagctgtt cagcgtcacc gccgctttct ccccaggcag ctccgtcttt
       61 tttaagtact ctttcaccga ttcccccagc gcgcccgtca gttgcgccgc gcttggcttg
      121 ttccgcttct tccaaaattc atccatccgt tttgcctcct cctgattttg tgtcgttttg
      181 tgttatcctg cgcttctttt tgcagagccg gtttttgtcc ccttgccctg taattccatt
      241 ttatactaac tgagcttgga taacgcacca acttttcagc gccccaaaac gcccgcaaaa
      301 tggccccaca ggcggctttg tgccctgtgg aaggtgcggc gaataataaa aaatagcctg
      361 tagctatttt ttacgcgcat ccgcccactc ccgtgggcgg gcgctcaaga ttttaaggcc
      421 gcaggctatt ttttcggcca tccgtttcgg ttgccgcgca gcggcgagaa acggggccat
      481 caagataaat tattttttat tgcgcagcaa tgaaaaaaga ccggcggaga aaatttctcc
      541 gccggtcttt ttagtgcagg caagctttca tttctgcggc ggcgagtcag gccgtcggcc
      601 gctgcaaaaa cttttttcat ctgcgccgca tcttgtgggt gaagaccgcg atcgccaccg
      661 ccgccatcaa gacgccgtac cccaataccc accagagatg gggcaggata tcggccgtcc
      721 ggcccgacag ggccgcccgc tgcagctcga cggcatggac gaaaggcagg gcgtaggcca
      781 cctttttaaa gccgcccccc accagctcaa gatcgaacca ggtgccggag agccaggccg
      841 ccacattggt caacagggcc ccgcagatac cgcccacctg cttatcattg aaaaggctgc
      901 cgcacaaaag gcccagcgcg ataaaaaaca gcgagacagg cagggtccac agcaccgacc
      961 agaggatctc cacccgcgct ttcaggccca gaaagagcgc cccggcatag cagacggcgc
     1021 tctggcccag cgccaggggc agcagcggca gcaggtagcc gccgatatag tccgctgcag
     1081 tcagcggcgt ggtatacagc cgctccagca gggcggtggt gcggtcccgc gagatgaggg
     1141 tggccgagaa cagcgtcaaa aaggaaaggc tgaacaccgc gatgccgggc gccagatggc
     1201 tgatctcaaa aaggctgacc gggatgttgg cctggatcgc cgagagcagc aaaagaagca
     1261 ccaggggaaa ccctgccccg aacgccgcgg tcagcgggtc acgcagaatc tcctttgccg
     1321 tccggctgga aaatgtgagc atcctcattg cagcggcccc tctttcac
//
LOCUS       DBMDYA010000741         1367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18159_length_1367_cov_1.135861, whole genome shotgun sequence.
ACCESSION   DBMDYA010000741 DBMDYA010000000
VERSION     DBMDYA010000741.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18159_length_1367_cov_1.135861"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(154..1305)
                     /locus_tag="ACZPFK_13790"
     CDS             complement(154..1305)
                     /locus_tag="ACZPFK_13790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR1407426.1"
                     /translation="MRWSVQADGLRCEFGALFGYVEATTARLREGVEQLLARGKVTLL
                     RGAAVLEAGRVVRVGEERCSAQHVLLAAGSRPASPPIPGSGLPGVVTSDDLLAGRGAD
                     CGSLVIIGGGVIGAEFAEIYTALGRRVTVLEMAPRLLPTLDREIGQNLAMILKKRGAE
                     IVVGAAVREIASADGGLCCRYEVKGETREASGERVLVCTGRVPCTSGLLGEGVELGSE
                     RGYIPVNERFETRVAGVWAVGDLVLGGVQLAHVAEAQAKNAVRAMFGAPGVKMLSAVP
                     ACVFTDPEIACAGLTADEAKAAGHAVTVKKSLTSANGRAVLEGAERGFAKLVFDAQDD
                     RLLGAQLMCPHAGEMIGGLTAAINAGLTAAQLRAAVWPHPTVSEILGGI"
BASE COUNT          231 a          518 c          414 g          204 t
ORIGIN      
        1 tatggccgtg gcccgaggac gggccgcacg gcaaaaaaac ttatcataat ggccccgttt
       61 ctcgccgctg cgcggcaacc gaaacgaatg gccaaaaaaa tagccgcata gcggctattt
      121 tttattgctt aaaaaccagg gctcctccgc gggtcagata ccgcccaata tctcgctcac
      181 cgtggggtgg ggccacacgg cggcgcgcag ctgcgcggcg gtgaggcccg cgttgatggc
      241 cgccgtcagg ccgccgatca tctcgcccgc atgggggcac atcagctgtg cgcccagcag
      301 ccggtcgtcc tgtgcgtcaa agaccagctt ggcaaagccg cgctccgccc cctccagcac
      361 ggcccggcca ttggcggagg tgagcgattt tttcactgtc accgcatgcc ccgcggcctt
      421 ggcttcgtcg gcagtcaggc ccgcgcaggc gatttccggg tcggtgaaaa cacaggcggg
      481 cacggcggaa agcattttga cgccgggcgc gccgaacatg gcccgcaccg cgtttttggc
      541 ctgcgcctcg gccacatggg ccagttgaac gccgcccaac accaggtcgc ccaccgccca
      601 aacgcccgcg acacgcgtct cgaagcgctc gttcaccggg atatagccgc gctcggagcc
      661 cagctccacc ccctcgccca gcaggccgct ggtgcagggg acgcgcccgg tgcagaccag
      721 cacccgctcg cccgacgcct cgcgcgtctc gcctttcacc tcatagcggc agcagaggcc
      781 gccgtccgcc gaagcgatct ctctcaccgc ggccccgaca acgatctctg cgccccgctt
      841 tttgagaatc atggcgaggt tctgcccgat ctcccggtcc agcgtgggca gcagccgggg
      901 cgccatctcc agcaccgtga cccgccgtcc cagggccgta tagatctctg caaactcggc
      961 gccgatgacg ccgccgccga tgatgacaag gctcccgcag tccgcgccgc ggcccgccag
     1021 caggtcgtcg ctggtcacca cgccgggcag cccgctgccc gggatcggag gggacgccgg
     1081 gcgcgagccc gcggccagca ggacatgctg cgcagaacag cgctcctctc ccacccgcac
     1141 gaccctgccg gcctccagca ccgcggcgcc gcgcagcagg gtgaccttgc cccgggcgag
     1201 cagctgctcg acgccctccc gcaagcgggc ggtggtcgcc tccacatagc cgaacagagc
     1261 gccgaattca cagcgcaggc cgtcggcctg cacgctccag cgcatcgtaa tacaactttg
     1321 tcgcttcaaa catcatcagg tcctgcttcc cgtacatgct gtccggc
//
LOCUS       DBMDYA010000742         1363 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18231_length_1363_cov_0.989699, whole genome shotgun sequence.
ACCESSION   DBMDYA010000742 DBMDYA010000000
VERSION     DBMDYA010000742.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18231_length_1363_cov_0.989699"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="ACZPFK_13795"
     CDS             <1..315
                     /locus_tag="ACZPFK_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_process: GO:0007007 - inner mitochondrial membrane
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR1407427.1"
                     /translation="FFGGEGFIMQKLSGSGKAFIEIDGHAVQYDLAPGQSMVVDTGYL
                     AAMSETCSIEVVTVPGVKNMLFGGEGVFNTVVKGPGRIILQSMPVSAVAGALRPFFPS
                     SN"
     gene            complement(525..776)
                     /locus_tag="ACZPFK_13800"
     CDS             complement(525..776)
                     /locus_tag="ACZPFK_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407428.1"
                     /translation="MLKMISPVLGLALVLFAAAAALLAWKRRSGRKRMEALAYQKMQR
                     EAERAYQAHLAQLCWNYRAKRTRHARRPGPGPDFYPKVS"
     gene            881..>1363
                     /locus_tag="ACZPFK_13805"
     CDS             881..>1363
                     /locus_tag="ACZPFK_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1407429.1"
                     /translation="MERGPLQEAHLQTLREYHLGPREAAGAVCLRYVAGETIIREGSG
                     LDYFFVIRRGRAKICTESRDGKALILCYYISEGVMGDIELMNDLRTATTSVVAVTDTE
                     CIALPYPQCAAPLKENPAFVRRLAEHLSAKLLQSSRSHCAAALCTAEERLCAYLLMAS
                     D"
BASE COUNT          234 a          429 c          478 g          222 t
ORIGIN      
        1 ttctttggcg gcgagggctt catcatgcag aagctctccg gcagcggcaa agcctttatc
       61 gagatcgacg gccacgcggt gcagtacgac cttgcgcccg gccagtcgat ggtggtggac
      121 accgggtacc tggcggccat gagcgagacc tgctcgatcg aggtggtgac ggtgccgggc
      181 gtgaagaaca tgctgttcgg cggcgagggg gtcttcaaca ccgtggtcaa gggcccgggc
      241 cgcatcatcc tgcagagcat gccggtgagc gccgtagcgg gcgcgctgcg gccgttcttc
      301 cccagcagca actgagcgcg ggggggcgga acagcggaca gagcggcatc cgcccgcgga
      361 gaacggccac ttgacccgat caactgaaac gaacgcccgt cccggcccgc cgcgctctgc
      421 ggcaggctgg gacgggcgtt cagcaaaagg ggagttgtca gggagaatcg ccgcggggtg
      481 ggcgcgcgct tttgatccgc cgcgcccctg cccacccccc ggccctacga gaccttgggg
      541 tagaaatccg gccccgggcc cggccgccgg gcgtgccggg tgcgcttggc gcgataattc
      601 cagcacagct gggccaggtg ggcctgatag gcccgctcgg cttcccgctg catcttttgg
      661 taagccagcg cttccatccg cttgcggccc gaccgccgct tccaggccag gagggcggca
      721 gcggcggcaa acaggacgag cgcgagcccc aaaacgggcg agatcatttt caacattgct
      781 gtcacctcct gtctttatta taggcgcggc cttgacagac ccataggaca tatgtcacac
      841 tgatgaaaag ccctttgatg aggcgaagga ggcggtcggc gtggagcggg gacccttgca
      901 ggaggcccat ctgcagacgc tgcgggagta tcatctcggc ccgcgggagg cggccggcgc
      961 ggtctgcctg cgctacgtgg cgggggagac gatcatccgc gagggaagcg ggctggacta
     1021 tttttttgtg atacggcggg gcagggccaa gatctgcacc gagagccggg acggcaaggc
     1081 gctgatcctg tgctattata tctcagaggg cgtgatgggg gatatcgagc tgatgaacga
     1141 tctgcgcacg gcgaccacca gcgtggtggc cgtgaccgac accgagtgca tcgcgctgcc
     1201 ctatccccag tgcgccgcgc cgctgaaaga gaaccccgcc tttgtgaggc ggctggccga
     1261 gcacctctcc gccaagctgc tgcaaagctc gcgcagccac tgcgccgcgg cgctctgcac
     1321 ggcggaggag cgtctttgcg cctatctgct gatggcctcc gac
//
LOCUS       DBMDYA010000743         1362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18244_length_1362_cov_1.293418, whole genome shotgun sequence.
ACCESSION   DBMDYA010000743 DBMDYA010000000
VERSION     DBMDYA010000743.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18244_length_1362_cov_1.293418"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            134..1180
                     /locus_tag="ACZPFK_13810"
     CDS             134..1180
                     /locus_tag="ACZPFK_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407430.1"
                     /translation="MDFAQFSDFAQARGCRQVQDDAFGIYRGYPFAVHYKKGGKGQLT
                     ATFKFGAKAPKSLLKEMRAALKPYGAAPMSYAPDRMGFLLSGKDGVDENFDGALSALA
                     ELFAAAGVTPPAACPFCKGQGCDTLAFVGGGYVPVHRTCLDRVLGGSAAAARHSMQQG
                     NYFTGILGALVGGLVGALPSLLTIWFASRVFALLFALIPICAYQGYKICRGKLNRVAT
                     VVTILSSILQVFALEQILFYLSVVDYYGIWPSIFSSMVLYFQIYTLGDMLASMITELV
                     FVALGIWISWRMITQTGGAALQGVQYVSATTAPYAGPGVAPASVQPPAPQAAPGVIPT
                     EAPLPEEDPCEWKG"
BASE COUNT          217 a          444 c          388 g          313 t
ORIGIN      
        1 gccatcttgc acgctttccc aactgctctg ttcttgtcag gaatagtgga atatagtata
       61 ctgtctttga taccgcgccg caggctctat ccggccacgg ccgtcacgat accataaaag
      121 ggaagtgtgt gaaatggatt ttgctcagtt ttccgacttt gctcaggcgc gcggctgccg
      181 tcaggtgcag gacgacgctt tcggcatcta tcgcggctac ccgtttgccg tccattacaa
      241 aaagggcggc aagggccagc tgaccgccac tttcaaattc ggcgccaaag cgccgaagtc
      301 cctgctgaaa gagatgcgcg ccgccctcaa gccctatggc gccgccccca tgtcctatgc
      361 ccccgaccgg atgggcttcc tcctgtccgg caaagacggg gtggacgaaa attttgacgg
      421 cgctctctcg gcgctggccg agctcttcgc cgccgcgggg gtgacgccgc ccgccgcctg
      481 tccgttctgc aaggggcagg gctgcgacac cctcgccttt gtcggcggcg gttatgtgcc
      541 ggtgcaccgc acctgccttg accgggtgct gggcggcagc gccgccgccg cccgccacag
      601 catgcagcag ggcaattact tcaccggcat cctgggcgcg ctcgtcggcg gcctggttgg
      661 cgccctgccc tcgctgctga ccatctggtt tgcctcccgg gtctttgcgc tgctctttgc
      721 cctcatcccc atctgcgctt accaggggta caagatctgc cgcggcaagc tcaaccgcgt
      781 cgccaccgtg gtgacgatct tgtcctccat cttacaggtg ttcgcgctgg agcagattct
      841 cttctacctg tcggtggtgg actattacgg catctggccc agcatttttt ccagcatggt
      901 cctctatttt cagatctata cccttggcga catgctcgcc agcatgatta ccgaactggt
      961 gtttgtggcg ctgggcatct ggatcagttg gcggatgatc acccagaccg gaggcgcggc
     1021 gctgcagggt gtgcagtacg tcagcgccac caccgccccc tatgccggcc ccggcgtggc
     1081 cccggcctct gtgcagcccc cggccccgca ggccgccccg ggcgtcatcc ccaccgaggc
     1141 ccccctcccg gaggaggatc cctgcgagtg gaaagggtga aagcgcgcgg tcgacgccgt
     1201 ttctccatct cctccatgct ctatctgcgg atcgcaggtc tttttttgtt tcagcaagag
     1261 atttgtccat aaattgtgag aattcaaaaa agcgccgctt tgctgccaaa ccagcagttt
     1321 gtggcgcttt tttctttatt tcttggtatg gaatcggttt ta
//
LOCUS       DBMDYA010000744         1359 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18289_length_1359_cov_1.401431, whole genome shotgun sequence.
ACCESSION   DBMDYA010000744 DBMDYA010000000
VERSION     DBMDYA010000744.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18289_length_1359_cov_1.401431"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1359
                     /locus_tag="ACZPFK_13815"
     CDS             <1..>1359
                     /locus_tag="ACZPFK_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR1407431.1"
                     /translation="SFPDRTRITFEGGITAVVGPNGSGKSNISDAIRWVLGETSSKQL
                     RGAGKMEDVIFGGTQTRSPMGFASVSLYIDNSAHRLEVEKDEVVITRKYYRSGESEYG
                     INGQNVRLKDIYELFLDTGIGRDGYSIIGQGRIAEIVGAKSGERREIFEEASGIAKYR
                     YRKNEAERKLAGAEDNLVRLRDILDELTARVGPLERDSEKAKKFLELSERRKSLEVTL
                     WADTLRKAKDSVREAQRAREAAQGDYDRASAEIEGVDAETEGIRAEIEQLIMDIERTN
                     AEIRSITEEIAGSDASIAVLRNDISHSEASIAGLRDEIAAAGEDDATVADEIAAKRAA
                     IEENAEKIRGQEARIAELSALLDELSASAEATGERRGVLTERLAGLTQRATDLKVAAA
                     GADSAAESAGARLSSLEDEVASGEERLQRLRQEQAENADYLARCTADLTRLGNIREGL
                     SLK"
BASE COUNT          260 a          440 c          497 g          162 t
ORIGIN      
        1 tccttccccg accgcacccg catcaccttt gagggcggca tcaccgcggt ggtggggccc
       61 aacggcagcg gcaagagcaa catctcggac gccatccgct gggtcctggg cgagacctcc
      121 tccaagcagc tgcgcggcgc gggcaagatg gaggacgtca tcttcggcgg cacccagacc
      181 cgcagcccca tgggctttgc gtcggtatcg ctgtacatcg acaactccgc ccaccggctg
      241 gaggtggaaa aggacgaggt ggtcatcacc cgcaagtatt accgcagcgg cgagagcgaa
      301 tacggcatca acgggcagaa cgtgcgcctc aaggacatct acgagctgtt tttggacacc
      361 ggcatcggcc gggacggcta ctccatcatc ggccagggcc gcatcgccga gatcgtgggc
      421 gcaaagtcgg gcgagcggcg ggagatcttt gaggaggcgt ccggcatcgc caagtaccgc
      481 taccgcaaaa acgaggccga gcgcaagctg gccggggccg aggacaacct ggtgcggctg
      541 cgggatatcc tggacgagct gaccgcgcgg gtgggcccgc tggagcggga cagcgaaaag
      601 gcgaaaaaat ttctcgagct gtccgagcgc cgaaaatcgc tggaggtcac cctctgggcc
      661 gacaccctgc gcaaggccaa ggacagcgtg cgcgaggccc agcgggcccg ggaggccgcc
      721 cagggcgact atgaccgcgc cagcgccgag atcgaggggg tggacgccga gacggagggc
      781 atccgcgccg agatcgagca gctcatcatg gatatcgagc gcacaaacgc cgagatccgc
      841 agcatcaccg aggagatcgc cggctccgac gcttccatcg cggtgctgcg caacgatatc
      901 tcccacagcg aggcctccat cgccggcctg cgggacgaga tcgctgcggc gggggaggac
      961 gacgccaccg tggcggacga gatcgcagcc aagcgggccg cgatcgagga gaatgcggag
     1021 aagatccgcg ggcaggaggc ccgcatcgcc gagctgtcgg cgctgctgga cgagctgtcg
     1081 gccagcgccg aggccaccgg cgagcggcgc ggcgtgctca ccgagcggct ggcgggcctg
     1141 acccagcggg ccaccgacct gaaagtggcc gccgccgggg ccgacagcgc cgccgagagc
     1201 gcgggggcgc ggctctcctc actggaggac gaggtcgcct cgggggagga gcggctgcag
     1261 cggctgcggc aggagcaggc ggagaacgcc gattatctgg cgcgctgcac cgcggacctg
     1321 acgcggcttg gcaatatcag ggaggggctg tccctcaag
//
LOCUS       DBMDYA010000745         1354 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18365_length_1354_cov_2.139665, whole genome shotgun sequence.
ACCESSION   DBMDYA010000745 DBMDYA010000000
VERSION     DBMDYA010000745.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18365_length_1354_cov_2.139665"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="ACZPFK_13820"
     CDS             complement(<1..406)
                     /locus_tag="ACZPFK_13820"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323810.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1407432.1"
                     /translation="MSVQESGKLHEECGVFGVYDKGEAGDVAALTYYGLYALQHRGQE
                     SCGMAINDDGVFTLHKDIGLVGDVFTPEKLRALGGGRMAVGHCRYATTGTRSRSNAQP
                     LVVRHIKGSMALAHNGNLTNAAELREKFELEGA"
     gene            complement(409..1119)
                     /gene="purC"
                     /locus_tag="ACZPFK_13825"
     CDS             complement(409..1119)
                     /gene="purC"
                     /locus_tag="ACZPFK_13825"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1407433.1"
                     /translation="MEKREQLYEGKAKKVYATDDSALVIVDYKDDATAGNGAKKGTIR
                     GKGVVNNKVTNSLMKMLEENGVPTHFVEQLSDRETVVKKVSIVPLEVIVRNVAAGSFT
                     ARLGVAEGTRFASPTLEYSYKDDALGDPLINSYHAIALGAATRQELDTIDAYAFKVNE
                     VLKAYLAKFNIDLIDFKLEFGRLPDGTIVLADEISPDTCRFWDATTGAHLDKDLFRRD
                     LGGEEDAYREVLKRLLGE"
     gene            complement(1179..1349)
                     /locus_tag="ACZPFK_13830"
     CDS             complement(1179..1349)
                     /locus_tag="ACZPFK_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HIR1407434.1"
                     /translation="MATVALNGAKNAAVLAAQMLAIHDARIAALLAEDREKMARSIAE
                     KDAAIRAEAAAL"
BASE COUNT          199 a          439 c          423 g          293 t
ORIGIN      
        1 tcgccccctc cagctcaaat ttttcccgca gctcggcggc gttggtcagg ttgccgttgt
       61 gagccagggc catgctgccc ttgatgtggc gcaccaccag cggctgcgcg ttgctgcggc
      121 tgcgggtgcc ggtggtggcg tagcggcaat ggcccaccgc catgcggccg ccgcccaggg
      181 cccggagctt ttccggcgtg aagacatcgc ccaccagccc gatgtccttg tgcagcgtga
      241 agacgccgtc gtcgttgatg gccatgccgc agctctcctg gccgcggtgc tgcagcgcgt
      301 agaggccgta gtaggtcaac gcggccacat cccccgcctc gcccttgtcg tagaccccga
      361 agacgccgca ctcctcgtgc agttttccgc tctcctgtac gctcatgctc actcccccag
      421 caggcgcttg aggacctcgc ggtaggcgtc ctcctcgccg ccgagatcgc ggcggaacag
      481 gtctttgtcc aggtgggcgc cggtggtggc gtcccagaag cggcaggtgt cggggctgat
      541 ctcgtccgcc agcacgatgg tgccgtcggg caggcggccg aactccagct tgaagtcgat
      601 gagatcgata ttgaatttgg ccagataggc tttcagcacc tcgttcacct tgaaagcata
      661 ggcgtcgatg gtgtccaact cctgccgggt ggccgcgccc agggcgatgg cgtgatagct
      721 gttgatcagc gggtcgccca gcgcatcgtc cttatagctg tactccaggg tggggctggc
      781 aaagcgggtg ccctcggcca cgcccaggcg ggcggtgaag ctgcccgccg ccacgttgcg
      841 cacgatgacc tccagcggca cgatgctcac ctttttgacc accgtctccc ggtcggagag
      901 ctgctcgaca aaatgggtgg gcacgccgtt ttcctccagc atcttcatca gcgagttggt
      961 gaccttgttg ttcaccacgc ccttgccgcg gatggtgcct ttcttggcgc cgttgcccgc
     1021 ggtggcgtcg tccttataat ccacgatgac cagggcggag tcgtcggtcg cgtagacctt
     1081 ttttgccttg ccctcataca gctgttcccg tttttccatg ttgttcttct ccctcttttg
     1141 tgttttataa aaataaaaag atatttggaa agcattcctt acagcgcggc cgcctcggcc
     1201 cggatggcgg cgtccttttc agcgatggag cgggccattt tctcccggtc ctccgccagc
     1261 agcgcggcga tgcgcgcgtc gtggatggcc agcatctgag cggccagcac ggcggcgttt
     1321 ttggcgccgt tcagcgcaac ggtggccaca ggaa
//
LOCUS       DBMDYA010000746         1350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18434_length_1350_cov_1.248199, whole genome shotgun sequence.
ACCESSION   DBMDYA010000746 DBMDYA010000000
VERSION     DBMDYA010000746.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18434_length_1350_cov_1.248199"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="ACZPFK_13835"
     CDS             complement(<1..177)
                     /locus_tag="ACZPFK_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1407435.1"
                     /translation="MEEKKKFYITTPIYYPSDKLHIGHTYCTVATDAMARYKRLKGYD
                     VMFLTGTDEHGIAFQ"
     gene            complement(224..1258)
                     /locus_tag="ACZPFK_13840"
     CDS             complement(224..1258)
                     /locus_tag="ACZPFK_13840"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR1407436.1"
                     /translation="MELLKPEQYGEADAFVSHHRSGSFMQSPAWAKVKPNWKHEIVAR
                     RGPDGKLKGTMSILIMPMGPGALMYAPRGPVCDYDDRDTLADLLAGAGAVAKKYHAHI
                     LKMDPYLLEGDKPHIEAFRSLGCAFTPDAGFKDTIQPRYNYMLPYLEGLTEEGLLAKF
                     ARETRYYIRYPGKHGVTCRWGDDCLDDFYKVYSETGERQGFNIRPKEYLAGFLKAFPA
                     HARMYMCYSEQGAPICGGLAVNYAGKTAHVYGCSSDDHELRKLRATYLLQWEMMKWAL
                     STGCWVYDMQGVAVRPEDSQQLYNVLGFKQNFTGEIVTTAGEFRIVYNAAVNAAMEAA
                     LKVRSKLAHR"
BASE COUNT          220 a          431 c          418 g          281 t
ORIGIN      
        1 ctgaaaggct atgccgtgct cgtcggtgcc ggtcaaaaac atcacgtcat agcctttcag
       61 gcgcttgtac cgggccatcg cgtcggtggc gacggtgcag taggtgtgcc cgatgtgcag
      121 tttgtccgac gggtaataga tcggcgtggt gatataaaac tttttcttct cttccatttt
      181 tctcccccca caatagatcg ggccaaggcc cgggttcaca ttttcagcgg tgcgccagct
      241 tgctgcgcac cttgagggcg gcctccatag ccgcgttgac cgccgcgttg tacacgatgc
      301 ggaactcgcc cgcggtggtg acgatctcgc cggtgaagtt ctgcttgaag cccagcacgt
      361 tgtacagctg ctggctgtcc tccgggcgca cggccacccc ctgcatgtcg tagacccagc
      421 agccggtgga gagggcccac ttcatcatct cccactgcag cagataggtg gcgcgcagct
      481 tgcgcagctc gtggtcgtcg ctggagcagc cgtagacgtg ggcggtcttg ccggcatagt
      541 tcaccgccag gccgccgcag atgggcgcgc cctgctcaga gtagcacatg tacatgcgcg
      601 cgtgggcggg gaacgccttc aaaaaaccgg ccagatactc tttcggccgg atgttgaagc
      661 cctgccgctc gccggtctca gagtagacct tgtaaaaatc gtccagacag tcgtcccccc
      721 agcggcaggt gaccccgtgc ttgccggggt agcggatgta gtaccgcgtc tcccgggcga
      781 actttgccag cagaccctcc tcggtgaggc cctccagata gggcagcata tagttgtagc
      841 gcggctggat ggtgtccttg aagcccgcgt cgggcgtaaa ggcgcagccc agcgatcgga
      901 acgcctcgat gtggggcttg tccccctcca gcagataggg gtccatcttc aggatgtggg
      961 cgtggtattt tttcgccacg gcgcccgcgc ccgccagcag gtcggccagg gtgtcgcggt
     1021 cgtcgtagtc gcagaccggg ccgcgcggcg cgtacatcaa agcgcccggg cccatgggca
     1081 tgatcaaaat gctcatcgtg cctttcagct tgccgtccgg cccgcgccgg gccacgatct
     1141 catgcttcca gttcggcttt actttcgccc aggccgggct ctgcataaag ctgcccgacc
     1201 ggtgatggct gacaaaggcg tccgcctcgc cgtattgctc cggtttcaaa agttccaaat
     1261 cttctgcgtc ccctttgtag agattcggcc ccggccgggc gtcgccgccg ccgggcggcc
     1321 ccgccgcttt tggcctatat actggtatag
//
LOCUS       DBMDYA010000747         1348 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18462_length_1348_cov_1.279070, whole genome shotgun sequence.
ACCESSION   DBMDYA010000747 DBMDYA010000000
VERSION     DBMDYA010000747.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18462_length_1348_cov_1.279070"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..583
                     /locus_tag="ACZPFK_13845"
     CDS             209..583
                     /locus_tag="ACZPFK_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1407437.1"
                     /translation="MKLRASGEDYLEAILVLQRQKGMVRSVDVARHLEVSKPSVSHAV
                     TTLKECGFLTMDEDFFLHLTDLGREIAEQTYEKHRFFTDLLTEAGVDPASAERDACRM
                     EHVISQKSFEHLRDSYNSHKED"
     gene            complement(646..975)
                     /locus_tag="ACZPFK_13850"
     CDS             complement(646..975)
                     /locus_tag="ACZPFK_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407438.1"
                     /translation="MNNPLDLFAWKLRSERKRQHLTQKALAERLHMSVRTIIEIEKCK
                     SNPKFETVALLAKELNISLDAAIFPDMVTGNVSKTVVDFFAGKSEAEIQKYIALCKQA
                     ETFQKDE"
BASE COUNT          331 a          331 c          333 g          353 t
ORIGIN      
        1 ttcacaaaat gctatccgat acattgtatc aacgattatt acagtgtaga ggggaactat
       61 ctcgacaagc gcatctgcca ttcttcttgt ccaaagcata tttggctggg tttttagaaa
      121 ttatccgttg caactacgat tttgaaacag cgcagagaag catccagata atggataaat
      181 ttatgcttct ttagattggg gggaaacctt gaaactccga gcgtcgggag aagattacct
      241 ggaggctatc cttgttctcc aaaggcaaaa gggcatggtg cgctccgtgg atgtcgcccg
      301 gcacctggag gtgtcaaagc ccagtgtgag ccatgccgtg accactctaa aagaatgtgg
      361 gttccttacg atggatgagg acttctttct tcatctgacc gacttagggc gagaaattgc
      421 cgagcagacc tatgagaagc accgcttttt cacggatttg ctcaccgagg ccggcgttga
      481 tccggcatcg gcggagcggg acgcctgccg gatggagcat gtcatcagcc aaaagtcctt
      541 cgagcatcta cgggattcct acaactcaca taaagaagat tgatttgccc gcatgaagat
      601 ggggtaaaac gaataacgcg gccgggcaca tcaaacccga ccgcgttatt cgtctttttg
      661 gaaggtctct gcctgtttgc agagcgcgat atatttctgg atctccgcct cacttttccc
      721 ggcaaagaaa tcaacgaccg tttttgagac gttcccggtc accatgtcag ggaagattgc
      781 agcatccagg ctgatattca attctttcgc cagaagcgcc acggtttcaa atttggggtt
      841 gcttttgcat ttctctatct caatgatcgt gcggacactc atgtggagcc gttcagctaa
      901 cgccttctgc gtcaagtgct gccgttttcg ctccgaccgc agtttccatg caaataaatc
      961 aagcggattg ttcactgcac atcacctcta tttcatttta ggatgtgcta tcgctcactt
     1021 aaataaccat gtaaatcatg ttatatgatg tttaactcat atttgagagt aaaggaagat
     1081 atggatgaga acagcaggga caaggaggtt aaaataaggc ggggttcgcc actcatgtgc
     1141 cggccctccc cgccatctcg aaaaatattc aaataacgaa gtagcgagcc atgctcatgg
     1201 aagtgagctg tggctcgctt tttatatatc agggctcatc cgtattgttc cccgctcttt
     1261 tgagcgccgg cgcctgggcg tgggatgccg ctccccgccc ccgtttcgtt ccgagtctct
     1321 ggcctgaacg aaacgaaagg agcttccg
//
LOCUS       DBMDYA010000748         1345 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18515_length_1345_cov_1.827974, whole genome shotgun sequence.
ACCESSION   DBMDYA010000748 DBMDYA010000000
VERSION     DBMDYA010000748.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18515_length_1345_cov_1.827974"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            63..>1345
                     /gene="aspS"
                     /locus_tag="ACZPFK_13855"
     CDS             63..>1345
                     /gene="aspS"
                     /locus_tag="ACZPFK_13855"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR1407439.1"
                     /translation="MKEIRGERLAGCATPERLAEMAGRQATVEGCVHNIRALGGIAFI
                     ILRTGRYLIQTVYDQSVCKDDVAALEDGCYVAATGTVRLEQRAAGGVELLLDGFEVLS
                     RPAAPYPLNMADRSLKATLGTNLEYRTVALRHPRERAVFRIMEGIENGFRAFMLQNDF
                     AEIHSPKITAISAEGGAEVFRLKYFGSDAVLAQSPQFYKQMGVAFFGRVFEVGEVYRA
                     ELHNTSRHLNEYVGLDFEMGYIRDMADVMEMETAMLRQVMDDLAKNYQPEIELLGVRL
                     PRVEAIPAVRFADALDYLKTVGGGKNRNDLTADDEVLLCSHILRQTGSEFVFVTHFPS
                     AKRPFYVMDDPQNPKEAFSFDLLFRGLEVTTGGQRIHDYDAQVAKMQARGLDPAQFES
                     YIGVHRCGLPPHGGLGIGLERLTMKLCGLSNIRDA"
BASE COUNT          253 a          389 c          467 g          236 t
ORIGIN      
        1 caggcgcttt gcccctgcgc gcggaccggg gcggtcacaa aagaggaaag gtgggaaaag
       61 tgatgaaaga gatcagagga gaaaggctgg ccggctgcgc gacgccggag cggctggcgg
      121 agatggcggg ccggcaggcc acagtggagg gatgcgtcca taatatccgc gcgctcggcg
      181 ggatcgcttt catcatcctg cgcaccgggc gctacctgat ccagaccgtc tatgaccaga
      241 gcgtctgcaa ggacgacgtg gcggcgctgg aggacggctg ttatgtggcg gccaccggca
      301 ccgtgcggct ggagcagcgg gcggcgggcg gcgttgagct gctgctggac ggctttgagg
      361 tgctctcccg cccggccgcg ccctacccgc tgaacatggc cgaccgcagc ctgaaagcga
      421 cgctgggcac caatctggaa taccgcaccg tggcgctgcg ccacccgcgt gagcgggctg
      481 ttttccgcat tatggagggc attgaaaacg gttttcgcgc gtttatgctg caaaacgatt
      541 tcgcagagat ccattcgccc aagatcacgg ccatcagcgc cgagggcggg gcggaggtct
      601 ttcggctgaa atatttcggc agcgacgccg tgctggccca gagcccgcag ttttacaagc
      661 agatgggggt ggcctttttt ggccgcgtgt tcgaggtggg cgaggtctac cgggccgagc
      721 tgcacaacac cagccggcat ctgaacgagt atgtggggct ggattttgag atgggctata
      781 tccgggacat ggccgacgtc atggagatgg agaccgccat gctgcggcag gtgatggacg
      841 atctggcgaa aaactatcag cccgagatcg agctgctggg ggtgcgcctg cccagggtgg
      901 aggccatccc ggccgtcagg ttcgcggacg cgctggatta tctgaaaacg gtggggggcg
      961 gcaaaaaccg caacgacctc accgccgacg atgaggtgct gctgtgcagc cacatcctgc
     1021 ggcagacggg cagcgagttt gtttttgtca cccacttccc ctcggcaaag cggccttttt
     1081 acgtcatgga cgacccccaa aatcccaagg aggcgttcag ctttgacctg ctgttccgcg
     1141 ggctggaggt gacgacaggc ggccagcgca tccacgatta tgacgcgcag gtggccaaga
     1201 tgcaggcccg cggattggac cctgctcaat ttgaaagcta tatcggcgta caccgctgcg
     1261 gcctgccgcc ccatggcggc ctgggcatcg ggctggagcg cctgaccatg aagctgtgcg
     1321 ggctgtccaa tattcgcgac gccag
//
LOCUS       DBMDYA010000749         1340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18591_length_1340_cov_1.513317, whole genome shotgun sequence.
ACCESSION   DBMDYA010000749 DBMDYA010000000
VERSION     DBMDYA010000749.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18591_length_1340_cov_1.513317"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="ACZPFK_13860"
     CDS             complement(<1..1014)
                     /locus_tag="ACZPFK_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1407440.1"
                     /translation="MDEKLFKQYAEFAVRVGVGVLPGQTLMITTPVEAAEFARCCAQA
                     AFEAGARDVQVRYEDEKLARIRYQNAAEQALCDVKPWVERSYLDYVEGAGGASILHIL
                     GEDPEAFLGLDAGKINRARQAARNALKTWRTYTMNDRVAWSIVAVPTDNWAKKVFPGC
                     GREQAVEKLWQAIFDVCRVTGGDPVGAWRRHIDTCTAMKEKLNALSLCSLRFTGGNGT
                     DLTVGLAEGAVWEGAASLTPQGQQFIANVPTEEVFTAPHRLRVEGIVYGTRPYVYNGD
                     LIEDFWVRFERGRVVESGARKNAALLETLLDTDEGSRSIGEVALVPATSPINRSGLLF
                     YNTL"
BASE COUNT          231 a          499 c          396 g          214 t
ORIGIN      
        1 cagggtgttg taaaacagca ggccgctgcg gttgatgggg ctggtggcgg gcaccagcgc
       61 cacctccccg atcgagcggc tgccctcgtc ggtgtccagc agcgtctcca gcagggcggc
      121 attcttccgc gcgccgctct ccaccacccg tccccgctcg aaccgcaccc aaaaatcctc
      181 gatcaggtcg ccgttgtaca catacggccg ggtgccgtag acgatcccct ccacccgcag
      241 ccggtggggt gcggtgaaga cctcctcggt gggcacgttg gcgatgaact gctggccctg
      301 cggggtcagg ctggccgcgc cctcccagac cgcgccctcc gccaggccca cggtgaggtc
      361 cgtgccgttg ccacccgtaa accgcagcga gcaaaggctc agggcgttca gcttttcttt
      421 catggccgtg caggtgtcga tgtgccgccg ccaggccccc accgggtcgc cgccggtcac
      481 ccggcagacg tcgaagatgg cctgccagag cttttccacc gcctgctccc gcccgcagcc
      541 gggaaagacc ttttttgccc agttatccgt gggcacggcc acgatgctcc aggccacacg
      601 gtcgttcatg gtgtaggtgc gccaggtctt cagcgcgttg cgcgcggcct gccgcgcgcg
      661 gttgatcttg cccgcatcca ggccgagaaa ggcctccggg tcctcgccca ggatatgcag
      721 gatcgaggcg ccgcccgcgc cctccacata gtccaggtag ctgcgctcca cccagggctt
      781 cacgtcgcag agcgcttgct ccgcggcgtt ctggtagcga atgcgagcca gcttttcatc
      841 ctcatagcgc acctgcacat cccgcgcgcc ggcctcaaag gccgcctgtg cgcagcagcg
      901 cgcaaactcc gccgcctcga cgggcgtggt gatcatcagg gtctgccccg gcagcacgcc
      961 cacccccacc cgcactgcga actccgcgta ttgtttaaaa agcttctcgt ccatcttctc
     1021 ccgccctctc tgttgagttt ccatcttagt atacaccaaa acgccccggg gaaaaaccgt
     1081 gccgccaccg atcctgcccc ccgcgcgccc tcccggccgc tgcggcgcgg gccgccgaaa
     1141 aatgcataaa cccgcatggc cgggaaacgc taacagcaaa atcaggggaa cgccgcggcc
     1201 gggcacggtg cgccgcgccc gccccgcgta ttttgaaagg aatgaatggg aatatgacgt
     1261 caaccacatc cagacagacg gcgggcgccc agacgggcaa acccgggtcc acccaaaagg
     1321 ccgcccccgc caaggcaaaa
//
LOCUS       DBMDYA010000750         1337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18628_length_1337_cov_1.180421, whole genome shotgun sequence.
ACCESSION   DBMDYA010000750 DBMDYA010000000
VERSION     DBMDYA010000750.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18628_length_1337_cov_1.180421"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(225..>1337)
                     /locus_tag="ACZPFK_13865"
     CDS             complement(225..>1337)
                     /locus_tag="ACZPFK_13865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1407441.1"
                     /translation="DGFGLLRFGERLTGPEYAANYIGLLGGKNISVRLRSFVTTIEKS
                     GDGFAVTYTSRGGLCRLYTAALVLATGCRERTARQIFVHGDKPAGVYTAGTAQHLVNL
                     QGVLPTRRCVILGSGDIGLIMARRLTLEGAQVEGVYEAKPEPSGLARNIRQCLTDFDI
                     PLHLSHTVTRVLGEGRVEAVETARVDERMKPVPGSERLVPCDALILSVGLIPENELAE
                     SLGIPLDPHTKGPAVDGRFETVCPGVFCCGNAAQVHDLVDYVSEGAATAGRCAALRAL
                     GKEAPAAPALAVEYGRAFVSVLPQRLQPGDAPVTCYFRVAETRGASRLTVSQGGAVLL
                     QKTYRHLRPPEMERVTLRPGAAGPVVFQLEDVGDDA"
BASE COUNT          200 a          495 c          449 g          193 t
ORIGIN      
        1 gggtgggcgc ggtgacctcc cgctcgccga acgccacccc ccgcgggcag cccgcgccgg
       61 tcaccgtgac ggcgccctcc cgctgggtca cggtcaaatc gcagccgttg gggcacacga
      121 tacaggtaaa aaatgcgctc tcctcgctca tttccctgca acgcctcctt tcatcctact
      181 gtgtggaatc atccaaagcg gggcgcacgc gccgccgcgc cgcctcacgc gtcatcgccc
      241 acatcctcca gctgaaagac gaccggccct gcggcgccgg gccgcagggt cacccgctcc
      301 atctcgggcg gccgcaggtg gcggtaggtc ttttgcagca gcaccgcgcc gccctggctg
      361 acggtgagtc ggctcgcgcc ccgggtctcc gccacccgga agtagcaggt caccggcgcg
      421 tcgccgggct gcagccgctg gggcaagacc gagacgaacg cgcggccgta ttccaccgcc
      481 agcgccgggg ccgccggggc ctctttgccc agcgcccgca gggccgcgca ccggcccgcc
      541 gtcgccgcgc cctcgctgac atagtccacc agatcgtgca cctgggcggc gttgccgcaa
      601 cagaagacgc cgggacaaac cgtctcaaag cgaccgtcca cggccgggcc cttggtgtgc
      661 gggtccagcg ggatgcccag ggattccgcc agctcgtttt ccgggatgag gcccaccgag
      721 aggatcaggg cgtcgcaggg gaccagccgc tccgagccgg gcacgggctt catgcgctcg
      781 tccacccgcg ccgtttccac cgcctccacc cgtccctcgc ccagcacccg ggtcacggtg
      841 tgggagaggt gcagcgggat gtcgaagtca gtgagacact ggcggatgtt gcgcgcaagg
      901 ccgctgggct ccggtttggc ctcatagacg ccctccacct gggcgccctc cagcgtcagg
      961 cggcgcgcca tgatcaggcc gatatcgcca ctgcccagga tgacgcagcg ccgcgtgggc
     1021 agcacgccct gcaggttgac caggtgctgg gccgtgcccg cggtgtagac cccggcgggt
     1081 ttgtcgccgt ggacgaaaat ctgccgggcg gtgcgctcgc ggcagccggt ggccagcacc
     1141 agcgccgcgg tgtacagcct gcacaggccg ccgcggctgg tgtaggtgac ggcaaagccg
     1201 tcgccgcttt tttcgatggt agtcacaaag ctgcgcagcc gcaccgagat gttcttgccg
     1261 cccaacaggc cgatatagtt ggcggcgtac tccgggccgg tgagccgctc gccgaagcgc
     1321 agcagcccaa agccgtc
//
LOCUS       DBMDYA010000751         1330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18735_length_1330_cov_1.301871, whole genome shotgun sequence.
ACCESSION   DBMDYA010000751 DBMDYA010000000
VERSION     DBMDYA010000751.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18735_length_1330_cov_1.301871"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="ACZPFK_13870"
     CDS             <1..531
                     /locus_tag="ACZPFK_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407442.1"
                     /translation="QVLTVTEGAGRDGRAEAALRIFENRGVYSEFLPALAVSPDLAEN
                     AVNLISAQLSQLPQTELVLAATPALARAAAEAAPGLPVACFGDDGGLQTLIRSGAVLA
                     AVSFDKAAAARTVAAFARNAADGKDPTDGTEARLDDARGTLVGFSVFSAPLPVAQAGE
                     PAAEDAALQEGQDDQE"
     gene            518..>1330
                     /gene="rlmD"
                     /locus_tag="ACZPFK_13875"
     CDS             518..>1330
                     /gene="rlmD"
                     /locus_tag="ACZPFK_13875"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1407443.1"
                     /translation="MTKNEIIELEITALSSDGNGVGRADGMAVFVPFTAPGDRALVKI
                     VKVQKSFAFGILHELLSPGPARVEPDCPVFGRCGGCALRQISYPAELAAKTAFVEDAF
                     RRIGGFSISAAPCLPSPQMDRYRNKAQYPLGADAEGRVFAGFFAPRSHRVIPCADCLL
                     QPQALGEVANLLCALFTEYRLPVYDEQRRTGLLRHLYLRRARDGELSVCIVANGRRLP
                     HEKELVRRLTQQRPEVKTVALNINTKNTNVILGGETRVLFGTGVLEDTLCGVP"
BASE COUNT          193 a          454 c          455 g          228 t
ORIGIN      
        1 caggtgctga cggtgaccga gggcgccggt cgggacggcc gcgccgaggc cgcgctgcgc
       61 atctttgaaa accggggcgt ttacagcgag tttttgcccg ctctggccgt ttcacctgat
      121 cttgccgaaa acgccgtcaa cctgatctcg gcccagctct cgcagctgcc gcagacggag
      181 ctggtcctgg cagccacccc ggcgctggcg cgggcggcgg ccgaggccgc ccccggcttg
      241 ccggtcgcct gctttggcga cgacggcggc ctgcagacgc tgatccgctc cggcgcggtg
      301 ctcgccgcgg tctcctttga caaagccgcg gcggcacgca ccgtcgccgc tttcgcgcgg
      361 aacgctgcgg acggcaagga cccgaccgac ggcaccgagg cgcggctgga cgacgcgcgc
      421 ggcacgctgg tcggtttttc cgtcttttcc gccccgctcc cggtggcaca ggcgggtgag
      481 ccggccgcgg aagacgccgc tttacaggag gggcaagatg accaagaatg agatcataga
      541 gctggagatc accgccctct ccagcgacgg caacggcgtg ggccgggccg acggcatggc
      601 ggtctttgtg cctttcaccg ccccggggga ccgggcgctg gtgaagatcg tcaaggtgca
      661 gaagagcttt gctttcggca tcctgcacga gcttctctcc cccggccccg cccgggtcga
      721 accggactgc ccggtgttcg gccgctgcgg cggctgcgcg ctgcgccaga tcagttatcc
      781 ggcggagctg gcggcaaaga ccgcttttgt ggaggacgca ttccgccgga tcggcggttt
      841 ttccatttcc gccgccccct gcctgccctc cccgcagatg gatcgctacc gcaacaaggc
      901 gcagtaccct ttgggcgcgg acgccgaggg gcgggtcttc gcgggctttt tcgcgccgcg
      961 cagccaccgg gtgatcccct gtgcggactg tcttttacag ccgcaggccc tgggcgaggt
     1021 ggcgaatctg ctctgcgccc tctttacgga ataccggctc cccgtctacg acgagcagcg
     1081 ccgtaccggt ctgctgcgtc acctttatct gcgccgcgcc cgggatgggg agctctcggt
     1141 gtgcatcgtg gcgaacggcc ggcgcctgcc ccatgaaaaa gagctggtgc ggcggctgac
     1201 ccaacagcgg ccggaggtga aaacggtcgc cctgaacata aacacgaaaa acaccaacgt
     1261 cattctgggc ggtgagaccc gcgttttgtt cggtaccggc gtgctggagg acacgctctg
     1321 cggggtgccg
//
LOCUS       DBMDYA010000752         1323 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18858_length_1323_cov_1.526187, whole genome shotgun sequence.
ACCESSION   DBMDYA010000752 DBMDYA010000000
VERSION     DBMDYA010000752.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18858_length_1323_cov_1.526187"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(274..>1323)
                     /locus_tag="ACZPFK_13880"
     CDS             complement(274..>1323)
                     /locus_tag="ACZPFK_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1407444.1"
                     /translation="AIARERGIAEAESQCVTGEELDAMPQEELDKKVLDLRVFARVSP
                     EHKVRIVKAFKANNKIVSMTGDGVNDAPSLKAADIGVAMGITGTDVAKGAADMVLTDD
                     NFATIEKAIEEGRGIYNNIKKTVIFLLGSNLGEVISMFTAIVAGLASPLKAVHILWVN
                     LITDTLPALALGADDKPMGIMKRKPRDPKDSLFAGGGLFLTIFYGALIAAMTLAAFLY
                     LPVSQLLASGAPVTVASIDALLTGELLIRAQTFAFTVLAVSQLWHSLGMRDVDTSIFI
                     TIRHENKLTLLSFFGGLLLQVCATEIPLFAGVFGTATLAFGEWLALIAFAMVPLVFHE
                     IFVPIRRALRVGERG"
BASE COUNT          254 a          398 c          415 g          256 t
ORIGIN      
        1 ctcttttagc ttggcctgca ctttttcgca attggcaagg gcggattcaa gaacgtggac
       61 cgtatccttg atctgctggt ccgtgaacag gctcaaaaaa atcgcctccg tttcgctgcg
      121 cctgttttta tggccgtggc ccaaggatga gccacaaggc cgaaaaaaca actgatccta
      181 atggccctgt ttctcgccgc tgccgcggca accgaaacag atggccgaaa aaataacctc
      241 cgctttgcgg aggctatttt ttaccgcggc gtctcagccg cgctcgccga cacgcagggc
      301 gcggcggatg gggacaaaga tctcatggaa cacaagcggt accatagcaa atgcaatcag
      361 cgccagccac tcgccaaagg cgagggtggc ggtgccgaag acccctgcaa agagcgggat
      421 ctcggtggcg cagacctgca gcagcaggcc gccgaaaaag ctgagcagcg tcagcttgtt
      481 ctcgtgccgg atggtgatga agatgctggt gtccacgtcc cgcatcccca ggctgtgcca
      541 cagctggctg accgccagga cggtgaaggc aaaggtctgg gcgcggatga gaagctcgcc
      601 ggtgagcagg gcgtcgatgg aagccactgt gaccggcgcg ccggaggcca gcagctggga
      661 cacgggcaga tagagaaaag cagccagcgt catggcggcg atgagcgcgc cgtagaaaat
      721 ggtcaaaaac aggcctccgc ctgcaaacag gctgtccttg gggtcgcggg gcttgcgctt
      781 catgatgccc atgggcttgt cgtccgcccc cagggccagg gcgggcagcg tgtcggtgat
      841 caggttgacc cacaggatgt gcaccgcttt cagcgggctt gccagcccgg ccacaatggc
      901 ggtgaacata ctgatgacct cgcccagatt cgagcccagc aaaaagatga ccgtcttttt
      961 gatgttgttg tagatgccgc ggccctcttc gatggccttt tcgatggtgg cgaaattgtc
     1021 gtcggtgagc accatgtccg ccgcgccctt tgccacatcg gtgccggtga tgcccatggc
     1081 cacgccgatg tcggcggctt tcaggctggg ggcgtcgttg acgccgtcgc cggtcatcga
     1141 gacgatcttg ttgtttgcct tgaacgcctt gacgatgcgc accttgtgct cagggctgac
     1201 ccgggcgaag acccgcaggt ccagcacctt tttgtccagc tcctcctggg gcatggcgtc
     1261 cagctcttcg ccggtcacac actggctctc cgcctcggcg atgccccgct ccctggcgat
     1321 ggc
//
LOCUS       DBMDYA010000753         1320 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18897_length_1320_cov_1.270714, whole genome shotgun sequence.
ACCESSION   DBMDYA010000753 DBMDYA010000000
VERSION     DBMDYA010000753.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_1320_cov_1.270714"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="ACZPFK_13885"
     CDS             complement(<1..209)
                     /locus_tag="ACZPFK_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR1407445.1"
                     /translation="MNKDPYAVLGLSSGASDDEVKAAYKKLAKKYHPDINASAEAEER
                     MKEINAAYDQIINHKNEPTGSYGGGG"
     gene            complement(206..1078)
                     /locus_tag="ACZPFK_13890"
     CDS             complement(206..1078)
                     /locus_tag="ACZPFK_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR1407446.1"
                     /translation="MFGYILANRDDLTPEQLARYRACYCGLCKTLGERYGLSGRAALT
                     YDMTFLVLLLSSLYKPECEQGQERCAIHPAKPHPYWRNRITEYAADMNVALAYYNCLD
                     NWRDDRSLPGFAEAKLLEKKCAAIRRRYPRQLRAVEYSLAELARLEAAKTAGPDAAAG
                     CFGTLMGELFVLCEDRWSVPLRRMGAALGKFIYILDAYDDLPRDERTGSYNPLSSMQG
                     PDLPRRCHDILTMLIAECAAEFEKLPVLEDAALLRNILYSGVWTKFVQIENKGKKKTA
                     PSGQKGGAPEGDDR"
BASE COUNT          223 a          417 c          414 g          266 t
ORIGIN      
        1 ccgcccccgc cgtagctgcc cgtgggctcg tttttgtggt tgatgatctg gtcgtaggcg
       61 gcgttgatct ctttcatccg ctcctccgcc tcggcggagg cgttgatatc ggggtgatat
      121 tttttggcca gttttttgta ggctgctttc acctcgtcgt ccgacgcgcc cgaagagagg
      181 cccaacaccg cataggggtc cttgttcatc tgtcgtctcc ttccggcgcg ccgccctttt
      241 ggccgctggg cgcggtcttc tttttgccct tgttttcgat ctggacaaat ttggtccaca
      301 cgcccgagta gaggatgttg cgcagcaggg ccgcgtcctc cagcacgggc agcttttcaa
      361 actcggcggc gcattccgcg atcagcatgg tgaggatgtc gtgacagcgg cgcggcaggt
      421 ccggcccctg catggacgac agcgggttat agctgccggt gcgctcgtcg cgcggcaggt
      481 cgtcgtaggc gtccaggata tagatgaatt tgcccagcgc cgcgcccatc cgccgcagcg
      541 gcaccgacca gcggtcctcg cagaggacga acagctcccc catcagggtg ccgaagcacc
      601 ctgcggccgc gtccgggccg gccgttttcg cggcctccag gcgggcgagc tcggccaggc
      661 tgtactccac cgcccgcagc tggcgcgggt agcggcggcg gatggcggcg cattttttct
      721 ccagcagctt ggcctcggca aagccgggca ggctgcggtc gtcccgccag ttgtccaggc
      781 agttgtaata ggccagcgcc acgttcatat cggcggcgta ttcggtgatg cggttgcgcc
      841 agtaggggtg cggctttgcc gggtggatgg cgcagcgctc ctgcccctgc tcgcactcgg
      901 gcttgtaaag ggaggagagc agcagcacca gaaaggtcat gtcgtaggtc agcgccgccc
      961 gcccggacag gccgtagcgc tcgcccagcg tcttgcaaag gccgcagtag caggcgcgat
     1021 agcgggccag ctgctccggc gtcaggtcat cccggttggc aaggatataa ccgaacatgc
     1081 gcgcgtcccc tttctgaggt gccctcactc taaaatagcc cgcagagcgg gcaaaagcga
     1141 tctcatcaga cctaataaat ataagtcgaa ggcgcgcgaa agtcaattct ttgcaaaaaa
     1201 ggtttacagc ccgttaatat tctgcccgaa aaaacgccgg cctcccgccg ctttttcaca
     1261 atccacggga aatcaaaaga gcccggcccc ctgcgcgttg cggcggaaaa acgcgccttt
//
LOCUS       DBMDYA010000754         1320 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18904_length_1320_cov_1.077112, whole genome shotgun sequence.
ACCESSION   DBMDYA010000754 DBMDYA010000000
VERSION     DBMDYA010000754.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18904_length_1320_cov_1.077112"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="ACZPFK_13895"
     CDS             <1..443
                     /locus_tag="ACZPFK_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013030.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407447.1"
                     /translation="DSCPDVGAFLGALEAGGIKKGLVLSPGDGIELLEPWLERLDYVL
                     LMFVQPGFSGQKFRPEVLAKLAALDSLRCERELPLLIEADGGIGWDNAAALVENGADL
                     LVSGVFASLDRRGDLGERTAVFRAVSAQRHGDRFTKHLFKGASV"
     gene            440..880
                     /gene="rpiB"
                     /locus_tag="ACZPFK_13900"
     CDS             440..880
                     /gene="rpiB"
                     /locus_tag="ACZPFK_13900"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1407448.1"
                     /translation="MKKIAMGCDHGGFELKEILRQRLQDEGYEVQDFGCYTPDPVEYP
                     EIAFKVAGEVAARPQEYEFGILCCGTGIGVSIAANKVKGVRAALCADCFSARMAKEHN
                     NANIITFGGRTTGRELAWEMVRAFMGAEFQHGIHEPRVEALTKA"
     gene            1007..>1320
                     /locus_tag="ACZPFK_13905"
     CDS             1007..>1320
                     /locus_tag="ACZPFK_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1407449.1"
                     /translation="MKKAAILRKIFISTLYLSTFTFGGGYVIVTLLKNKFVNELHWID
                     EEEMLDLVAIAQSSPGAIAVNGAIVVGYKLAGLAGVVCAVLGAVIPPFVILTLVSFFY
                     AA"
BASE COUNT          232 a          378 c          471 g          239 t
ORIGIN      
        1 tggacagctg cccggacgtg ggcgcctttc tcggcgcgct ggaggcgggg ggcatcaaaa
       61 aggggctggt gctctcgccc ggggacggga tagagctgct ggagccctgg ctggagcggc
      121 tggactatgt gctgctgatg ttcgtgcagc ccggcttttc ggggcagaag ttccgccccg
      181 aggtgctggc caagctggcg gcgctggact cgctgcgctg cgagcgggag ctgccgctgc
      241 tgatcgaggc ggacggcggc atcggctggg acaacgccgc cgccctggtg gaaaacggcg
      301 ccgacctgct ggtctcgggc gtgttcgcct ccctggaccg gcgcggcgat ctgggcgagc
      361 gcacggcggt ctttcgcgcc gtgagcgccc agcgccacgg cgaccgattc accaaacatc
      421 tgttcaaggg ggcatccgta tgaaaaaaat cgcgatgggc tgcgaccacg gcggctttga
      481 gctcaaggag atcctgcgcc agcggctgca ggacgagggg tacgaggtgc aggatttcgg
      541 ctgctacacg ccggacccgg tggagtaccc ggagatcgct ttcaaggtgg cgggcgaggt
      601 ggcggcgcgg ccgcaggagt acgagttcgg catcctgtgc tgcggcaccg gcatcggcgt
      661 ctccatcgcc gccaacaagg taaagggcgt gcgcgccgcg ctctgcgccg actgcttctc
      721 ggcccgcatg gcaaaagagc acaacaacgc caacatcatc acattcggcg gccgcaccac
      781 cgggcgcgag ctggcctggg agatggtgag agcctttatg ggggccgagt tccagcacgg
      841 catccacgag ccgcgggtcg aggcgctgac aaaagcctga accggcggtc ttgcgcaaaa
      901 gaggtcggga cccgtcggct ttggccggcg ggttcccttt ttggcaaaaa gagactacaa
      961 tggaaaggta cttttacagc ggcagctgag caaaggggga gatgccttga aaaaagcggc
     1021 gattttgcga aagatcttta tttccacgct ctatctgagc acatttacat tcggcggcgg
     1081 atatgtgatt gtgacgctgc tgaaaaacaa gttcgtcaat gagctgcact ggatcgatga
     1141 ggaggagatg ctggacctgg tggccatcgc ccagtcctcg ccgggggcca tcgcggtcaa
     1201 cggcgccatc gtggtgggct ataagctggc gggcctggca ggggtggtct gcgcggtgct
     1261 gggcgcggtg atcccaccct ttgtcatcct gacgctggtc tcgtttttct acgcggcgtt
//
LOCUS       DBMDYA010000755         1314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18991_length_1314_cov_1.508656, whole genome shotgun sequence.
ACCESSION   DBMDYA010000755 DBMDYA010000000
VERSION     DBMDYA010000755.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18991_length_1314_cov_1.508656"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="ACZPFK_13910"
     CDS             <1..1022
                     /locus_tag="ACZPFK_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407450.1"
                     /translation="PDDRYIYLIGSDNIWLEAALQSCNQAGLYPILLCNQAYHTFDAD
                     YSTVCLDVVNSMRRLVEILSARGLSRVALYGVNPQSVSDEARQEGYLTARAREGAGKG
                     KSDVFFNNGSLENCFEIFLRRSGEYDAAICVNEFAAVSLARRLARRDPERAKSLLIVS
                     CADGWLSQFYEGRILAVKGNFAARAKAAVALLENLRKNPDLSHIIMTIRWDFSALQPG
                     AEGPHAPPQGQSYPPLPWIQDAFYEDSELNEMMLVERLLRESTDLDRALLWHLLQGEP
                     YQKISEKCFLTESAIKYRIKKMVELCRVEGRQQLVALLNEYLPQPLPGGREGLYPARR
                     RRGGR"
     gene            1062..>1314
                     /locus_tag="ACZPFK_13915"
     CDS             1062..>1314
                     /locus_tag="ACZPFK_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407451.1"
                     /translation="MVLILVEPSYSRSIWCLNLLDGLTGEFKQKRVPFRQIASLDEAG
                     PDDRYIYLIGSDNAWVRAALAACNRMGIYPILLCNQAYHT"
BASE COUNT          234 a          416 c          446 g          218 t
ORIGIN      
        1 ggccggacga ccgctacatc tatctcatcg gctcggacaa catctggctc gaggccgcgc
       61 tccagtcctg caaccaggcg gggctgtatc cgattttgct gtgcaaccag gcctatcaca
      121 cctttgacgc cgattacagc accgtctgcc tggatgtggt caactcgatg cggcggctgg
      181 tggagatctt gtccgcccgc gggctgagcc gggtggccct gtacggcgtg aacccgcagt
      241 ccgtctcaga cgaggcgcgt caggagggct acctcaccgc ccgggcgcgg gagggcgccg
      301 gcaagggaaa aagcgacgtg tttttcaaca acggctcgct ggaaaactgc tttgagatct
      361 ttctgcgccg cagcggcgag tatgacgccg cgatctgcgt caatgagttc gctgccgtgt
      421 cgctggcgcg gcggctggcc cgccgcgacc cggagcgggc caagagcctg ctgatcgtca
      481 gctgtgccga cggctggctg tcccaatttt acgaggggcg cattttggcg gtgaagggca
      541 actttgccgc gcgggccaag gcggcggtgg cgctgctgga gaacctgcgc aaaaaccccg
      601 acctgtccca tatcatcatg acgatccgct gggatttcag cgccctgcag ccgggggccg
      661 aggggccgca tgcgccgccg caggggcaga gctacccgcc gctgccctgg atacaggacg
      721 cgttctatga ggacagcgag ctgaacgaga tgatgctggt ggagcggctg ctgcgggaga
      781 gcaccgacct ggaccgggcg ctgctctggc acctgctgca gggcgagccg taccaaaaga
      841 tatccgaaaa gtgctttttg accgagagcg ccatcaaata ccggatcaaa aaaatggtgg
      901 agctgtgccg ggtcgagggc cgccagcagc tggtggccct gctcaacgag tacctgcccc
      961 agccgctgcc cggcggccgc gaggggcttt atcccgcccg gcgccgccgg ggcggcagat
     1021 gacgcagccc accgcccgcc ggtgaggaaa ggaggacgca gatggtcctg atcctggtgg
     1081 agcccagcta ttcccgcagc atctggtgcc tgaacctgct ggacggcctg acgggcgagt
     1141 tcaagcaaaa gagggtgccg ttccggcaga tcgcctcgct ggacgaggcg gggccggacg
     1201 accgctacat ctatctcatc ggctcggaca acgcctgggt gcgggcggcc ctggcggcct
     1261 gcaaccggat ggggatctat cccatcctgc tgtgcaacca ggcctaccac acat
//
LOCUS       DBMDYA010000756         1314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18996_length_1314_cov_1.362737, whole genome shotgun sequence.
ACCESSION   DBMDYA010000756 DBMDYA010000000
VERSION     DBMDYA010000756.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18996_length_1314_cov_1.362737"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="ACZPFK_13920"
     CDS             complement(<1..606)
                     /locus_tag="ACZPFK_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR1407452.1"
                     /translation="MEKQFDLDALELLIDTRRFRALRDALAEMNEVDIAQFLGELEPE
                     KAVLAFRTLPKEAASEVFSNLESEVQQVIIGSITDAELAAIVEDLYVDDAVDMLEELP
                     ANVVKRVLKNAKPDTRALINQFLKYPENSAGSIMTAEFTDLRAAMTVAQAIEHIRRTG
                     EDRETIYTCYVIDASRKLEGVVTVKDLLLAPDGMQVRDVMSD"
     gene            complement(1106..>1314)
                     /locus_tag="ACZPFK_13925"
     CDS             complement(1106..>1314)
                     /locus_tag="ACZPFK_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407453.1"
                     /translation="RDMGDLDRLTKAQLCEELGVPLRSLNRVAAGCRGLVEYRGKRFY
                     ALPQLGDGGPPGLGADGCESAGGR"
BASE COUNT          219 a          460 c          413 g          222 t
ORIGIN      
        1 gtcgctcatc acgtcccgca cctgcatccc gtccggggcc agcagcaggt ctttcaccgt
       61 caccacgccc tccagcttgc ggctggcgtc gatgacatag caggtgtaga tggtctcgcg
      121 gtcctcgccg gtgcggcgga tgtgctcgat ggcctgggcc accgtcatcg cggcccgcag
      181 gtcggtgaac tcggcggtca tgatgctgcc cgccgagttt tcggggtact tcaaaaactg
      241 gttgatcagc gcccgggtgt ccggcttggc gttcttcagc acccgcttga ccacgttcgc
      301 gggcagctcc tccagcatat ccaccgcgtc gtccacatag aggtcctcca cgatggcggc
      361 cagctcggcg tcggtgatcg agccgatgat gacctgctgc acctcgctct ccaggttgga
      421 gaacacctcg ctggcggcct ccttgggcag ggtgcggaac gccagcaccg ccttttccgg
      481 ctccagctcg cccaggaact gggcgatgtc cacctcgttc atctcggcca gcgcgtcgcg
      541 cagcgcccga aaccggcggg tgtcgatgag cagctccagc gcgtccagat caaattgctt
      601 ttccatgggt ctcttcctcc tgttcgtttc aaagtttcgt ggcaggaaga caggacaaaa
      661 cgaaaacacc gcgaaagaca gaggtctctc gcggtgatcc acacaaaagc gcgcgccgcc
      721 ggcgcgggga agcagccccc ggcggagcgg ggcgcagccc ggcgcgggcg cacagaggca
      781 ggcggccgcg ccgccgctca gatcaaaaca gggcggaggc aagcggccgg cgtcggcgcg
      841 cggcataagt gaggatcatc gcaaaagcct gtacttcgac caacagcgtc cacttgttct
      901 ccctcctttg aattcgggtc aaaagcccct tggggcacct aacagtgtat gccctcccgg
      961 ccggttttgc aacccttttt tcgaaaaatt tgacggtggc aagggccgga aaaaagagcg
     1021 gccccgccgg gcggccgcgc ccctgccgtg ccccgggcgg gaagagcgcg cttttgcgcc
     1081 gacgcggctg gagcaagccc ccttgtcacc ggccgcccgc gctctcgcag ccgtctgccc
     1141 ccagcccggg cgggccgccg tcccccagct gcggcagggc gtaaaagcgc ttgccccggt
     1201 attccacaag gccgcggcag cccgccgcca cgcggttgag gctgcgcagg ggcaccccca
     1261 gctcctcaca gagctgggcc ttggtcaaac ggtccagatc gcccatgtcc cggt
//
LOCUS       DBMDYA010000757         1314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_18998_length_1314_cov_1.342127, whole genome shotgun sequence.
ACCESSION   DBMDYA010000757 DBMDYA010000000
VERSION     DBMDYA010000757.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_1314_cov_1.342127"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="ACZPFK_13930"
     CDS             complement(<1..530)
                     /locus_tag="ACZPFK_13930"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR1407454.1"
                     /translation="MAKQKVCVLFGGVSSEHEVSLMSASSVIRNIPADRYEVIPVGIT
                     KKGRWLYYPGPVANLADGSWEQYPDCVPAFISPDATDRGIVKCVDGAFTLQKVDVVFP
                     VLHGKGGEDGTVQGLLELAGIPYVGCGVLSSALCMDKAAANLVMDAAGIDRCEWDYMT
                     RAELPGFDAVADRLER"
     gene            complement(785..1114)
                     /locus_tag="ACZPFK_13935"
     CDS             complement(785..1114)
                     /locus_tag="ACZPFK_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1407455.1"
                     /translation="MNSKSLGKNLRSYRAKRGWSIKECAEKIGISTRYLADIERGDKI
                     PKLETFVHIVNTLSASADDVLQDSLGVGYLPKSNYIIRKLDGLDPVRKKQALDILDSV
                     ISSLKEN"
BASE COUNT          256 a          394 c          408 g          256 t
ORIGIN      
        1 ctgcgctcca gccggtcggc gacggcgtcg aacccgggca gctcggcccg ggtcatgtag
       61 tcccactcgc agcggtcgat gcccgcggcg tccatcacca ggttggccgc cgccttatcc
      121 atgcacagcg cgctggacag cacgccgcag cccacatagg ggatgcccgc cagctccagc
      181 aggccctgca cggtgccgtc ctcgccgccc ttgccgtgca gcaccgggaa gaccacgtcc
      241 accttttgca gcgtgaacgc gccgtccacg catttgacga tgccgcggtc ggtggcgtcc
      301 gggctgatga aagcgggcac acagtcgggg tactgctccc agctgccgtc cgccaggttg
      361 gcgacggggc ccgggtagta cagccagcgc ccctttttgg tgatgccgac ggggatcacc
      421 tcgtaccggt cggcggggat gttgcggatc accgaggaag ccgacatcag cgacacctcg
      481 tgctcgctgg agacgccccc gaagaggacg cagacctttt gctttgccat gatctcagaa
      541 tactccttcc cgatggggaa aaacagccgg tgcggcccgg ccccgccgcg ctgcgcgctg
      601 cggcccaaaa cgcgggcgcg ggcaggggcg cccggcgcgc ggtcaaaatt gcagatacag
      661 atatatatta ccataccgcg cgggttatga aaagggtgcg gcagggggcg cggccctttt
      721 tttgcccggc ggcccggcgg cgcggcagac aaaaagcctt tgagccgcgg gcgctcaaag
      781 gctttcagtt ttctttcagg gaggagatga cgctgtcgag gatgtccagc gcctgttttt
      841 tgcgcaccgg gtccagaccg tccagctttc tgatgatgta gttgctcttg gggaggtagc
      901 ccacccccag gctgtcctgc agcacgtcgt ccgcggaggc ggaaagggtg ttcacgatgt
      961 ggacgaaggt ctccagcttg ggtattttgt ccccgcgctc gatgtccgcc agatagcggg
     1021 tgctgatgcc gatcttctcg gcgcactcct tgatgctcca gccccgtttt gccctgtagg
     1081 acctcagatt tttccccagg cttttggaat tcaccagcct cgccgcctat cttgggatat
     1141 tttctacaac tagcatacaa attatacaaa atggaagaaa cgatctattc gtagaaaagt
     1201 ccctaaatag atgttgtttc tggaaaaaag atgtgctaac attggatgaa aagggcgggg
     1261 tcaccgagaa agaactggaa caggagagaa gaagatgata aagtcgacgg gtat
//
LOCUS       DBMDYA010000758         1312 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19038_length_1312_cov_1.554913, whole genome shotgun sequence.
ACCESSION   DBMDYA010000758 DBMDYA010000000
VERSION     DBMDYA010000758.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19038_length_1312_cov_1.554913"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="ACZPFK_13940"
     CDS             complement(<1..1156)
                     /locus_tag="ACZPFK_13940"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1407456.1"
                     /translation="MTYEKLEGLLRESGRSYDMGLIGRAYEVASKAHHGVKRRSGEPY
                     ITHPLSVAALLIDLGLDSASIAAALLHDVVEDTEITKADVKNEFGEEVANLVDGVTKL
                     GQITFSTMEEQQAENLRKMLLAMSQDIRVMLIKLCDRLHNMRTMAAMPDQKRRDKALE
                     TMEVYAPIAHRLGMNNVQEELEDRSLSFLDPVGYQDIVSLLERHGGRGEFIADVSRQI
                     RQRLAENGIEEAQLSSRVKSIYGIYRKMYIQNKEFEEIYDVYALRIIVETVAECYNAL
                     GVIHDMYHPLPNRFKDYISTPKPNGYQSLHTTVLGHEGIPFEVQIRTWDMHHTAEYGV
                     AAHWKYKAGIQGPDKLEERLAWVRQLLESQRDSEDAVDLLRNIKSELLPEE"
BASE COUNT          199 a          441 c          401 g          271 t
ORIGIN      
        1 cctcctcggg cagcagctcc gatttgatgt tgcgcagcag gtccaccgcg tcctcagagt
       61 cccgctggct ctccagcagc tggcgcaccc aggccagccg ctcctccaac ttatccggcc
      121 cctggatgcc ggccttgtat ttccagtgcg cggcgacgcc gtactcggcg gtgtgatgca
      181 tgtcccaggt gcggatctgc acctcgaaag ggatgccctc gtgccccagc acggtggtgt
      241 gcagcgactg gtaaccgttg ggcttggggg tggagatata gtccttaaag cggttgggca
      301 gcgggtggta catgtcgtgg atcacgccca gggcattgta gcactcggcc accgtctcca
      361 cgatgatgcg cagcgcatag acgtcgtaaa tctcctcaaa ctctttgttt tggatgtaca
      421 tcttgcggta gatgccgtag atggacttga cccggctgga gagctgggcc tcttcgatgc
      481 cgttttccgc cagccgctgc cgtatctggc gggacacgtc ggcgatgaac tcgccgcggc
      541 cgccgtgccg ctccagcagc gagacgatgt cctgatagcc caccgggtcg agaaaagaga
      601 ggctgcggtc ctccagctcc tcctgcacgt tgttcatgcc cagacggtgg gcgatggggg
      661 cgtagacctc catggtctcc agggccttgt ccctgcgctt ctgatccggc atggccgcca
      721 tggtgcgcat attgtgcagc cggtcgcaga gcttgatcag catcacgcgg atgtcctggc
      781 tcatggccag cagcatcttg cgcaggtttt cggcctgctg ctcctccatg gtggagaacg
      841 tgatctgccc cagcttggtc accccgtcca ccaggttggc gacctcttcg ccgaactcgt
      901 tcttcacgtc cgccttggtg atctcggtgt cctccaccac gtcgtgcagc agggccgccg
      961 cgatgctggc ggaatccagc cccaggtcga tgagcagcgc cgccacgctc agcgggtggg
     1021 tgatataggg ctcgccgctg cgccgcttga cgccgtgatg agccttggag gcgacctcat
     1081 aggcccggcc gatcagcccc atgtcatagc tgcggcccga ctccctcagc agcccctcga
     1141 gtttttcata ggtcatccac tcttccactc cggtcaccct ctcctcttcc ccaacacggc
     1201 gggacgcgcc ccgccggccc ctgcggcgcg ggccggcctc agccggtgag cgcgatcagg
     1261 gtgggcgagg acatcaggtc ccgcttttca tcgaccgggt gttccacata cc
//
LOCUS       DBMDYA010000759         1309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19098_length_1309_cov_1.607616, whole genome shotgun sequence.
ACCESSION   DBMDYA010000759 DBMDYA010000000
VERSION     DBMDYA010000759.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19098_length_1309_cov_1.607616"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1309)
                     /gene="groL"
                     /locus_tag="ACZPFK_13945"
     CDS             complement(<1..>1309)
                     /gene="groL"
                     /locus_tag="ACZPFK_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072050.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1407457.1"
                     /translation="LKDPFENMGAQLVKEVATKTNDAAGDGTTTATVLAQALVHEGMK
                     NVAAGANPMDLKRGMKKAVDAAVEAIKANSQKVSGSADIAKVATISASDAEIGTLISE
                     AMEKVTADGVITIEESKTADTYSEVVEGMMFDRGYITPYMATDTEKMEAVMDEPYILI
                     TDKKISSIQDLLPILEQIVKMGKKLVIIAEDVEGEALSTLIVNKLRGALNCVAVKAPG
                     FGDRRKEMLQDIAILTGGQVISEELGLELKDATVDMLGNAKQVKVTKENTVIVDGAGD
                     KGAISDRVAQIRAQIEETTSDFDREKLQERLAKLAGGVAVIRVGAATEVEMKEKKLRI
                     EDALSATKAAVEEGIVAGGGTILVDVIPDVMKATEALEGDEKTGAKIVINALREPLRQ
                     IVKNAGLEGSVVVDKIENSGKVGYGFNAQTEAYGMMLEDFGVVD"
BASE COUNT          176 a          426 c          415 g          292 t
ORIGIN      
        1 gtcgacaacg ccaaagtcct ccagcatcat gccgtaagcc tcggtctggg cattgaagcc
       61 gtagcccacc ttgccgctgt tctcgatctt gtcgaccacg acgctgccct ccaggcccgc
      121 gttcttgacg atctggcgca gcggctcgcg cagggcgttg atgacgatct tggcgccggt
      181 cttctcgtcg ccctccagcg cctcggtggc tttcatgaca tcggggatga catccaccag
      241 gatggtgccg ccgcccgcga cgatgccctc ttccacggcc gccttggtcg cagagagcgc
      301 gtcctcgatg cgcagcttct tctctttcat ctccacctcg gtggccgcgc cgacgcggat
      361 gaccgccacg ccgccggcca gcttggccag acgctcctgc agcttctcgc ggtcgaagtc
      421 ggaggtggtc tcctcgatct gggcgcggat ctgcgccacg cggtcggaga tagcgccctt
      481 gtcgccggcg ccgtccacga tgacggtgtt ctccttggtg accttgacct gcttggcatt
      541 gcccagcata tcgacggtgg cgtctttcag ctcgaggccc agctcttcgc tgatgacctg
      601 gccgccggtg aggatggcga tatcctgcag catctccttg cggcggtcgc cgaagcccgg
      661 ggccttgacg gcgacgcagt tcagggcgcc gcgcagcttg ttgacgatca gggtggacag
      721 ggcctcgccc tccacatcct cggcgatgat gaccagcttt ttgcccatct tgacgatctg
      781 ctcgagaatg ggcagcagat cctggatgga ggagatcttt ttgtcggtga tgaggatgta
      841 gggctcatcc atgacggctt ccatcttctc ggtgtcggtg gccatatacg gggtgatata
      901 gccgcggtcg aacatcatgc cctcgaccac ctcggagtag gtgtcggcgg tcttggactc
      961 ctcgatggtg atgacgccgt cggcggtgac tttctccatc gcctcggaga tcagggtgcc
     1021 gatctcagcg tcgctggccg agatggtggc gaccttggcg atgtcggcgc tgccgctgac
     1081 cttctggctg ttggccttga tggcctcgac ggccgcgtcg acggctttct tcatgccgcg
     1141 cttgaggtcc atcgggttcg cgcccgcggc aacgttcttc atgccctcgt gcaccagcgc
     1201 ctgggccagg acggtggcgg tggtggtgcc gtcgcccgcc gcgtcgttgg tcttggtggc
     1261 gacctctttg acgagctgcg cgcccatgtt ctcaaacggg tctttcagc
//
LOCUS       DBMDYA010000760         1306 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19159_length_1306_cov_1.153527, whole genome shotgun sequence.
ACCESSION   DBMDYA010000760 DBMDYA010000000
VERSION     DBMDYA010000760.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19159_length_1306_cov_1.153527"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1306)
                     /locus_tag="ACZPFK_13950"
     CDS             complement(<1..>1306)
                     /locus_tag="ACZPFK_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407458.1"
                     /translation="PAGQPLFYLIANIVLLLVAMVVNYNTIACGLSSIFSSPSPDLLP
                     ALASVAALVQCGVFLTGAEKFGASNYTVFAGVAVLCLAGNTFGKCITARMVAENFEMA
                     SSGFDHAAAYQVADEELVKRVTDGLGEPDPHLLVSRPTALVRGFLRQSFSEHRSDRVA
                     KWLGLAGLAGGIACAVIAGMLTKEPFTAVSCFAGAVCLAAPLSSTLVGAVPAGLMQAS
                     AARVGAVVPGPSAVEDLKNTNVVLVGARDLFPPSTVRLRGIKTFQKERIDLAILYAAS
                     ILVEGCDTLRDIFLGVVEGKRDMLYPVENLVTEVGYGFLGWIDNNRVIVGSREMMARH
                     DIEIPSMDYEKRYTGDDKQPIYLAVAGRLFGMFLVSYGPDEDMAETVEELRHAGISLL
                     VKSSDFNVTSELVAACYGVSPENVKVLSGAELTALTPSLSYLP"
BASE COUNT          245 a          444 c          421 g          196 t
ORIGIN      
        1 cgggcaggta ggacagcgag ggagtgaggg cggtgagctc ggcgcccgag agcactttca
       61 cgttttcggg gctgacgccg tagcaggccg ccaccagctc gctggtgacg ttgaaatcgc
      121 tggatttgac cagcagcgag atgcccgcgt ggcgcagctc ctccaccgtc tcggccatgt
      181 cctcatccgg gccgtagctg accaggaaca tcccgaacag ccgcccggcc accgcgaggt
      241 agatgggctg cttgtcgtcc ccggtatacc gcttctcgta atccatcgag gggatctcaa
      301 tgtcgtggcg ggccatcatc tcgcggctgc ccacaatgac ccggttgttg tcgatccagc
      361 ccaaaaagcc gtagcccacc tcggtgacca ggttctcaac cgggtagagc atatcgcgct
      421 tgccctcgac cacacccaaa aagatgtccc gcagggtgtc gcagccctcc accaaaatgc
      481 tggccgcata gaggatggcc aggtcgatgc gctctttctg gaacgtcttg atgccgcgca
      541 gccgcacggt ggagggcggg aacagatccc gcgcgcccac cagcaccacg ttggtgttct
      601 tcaggtcctc caccgcgctg gggccgggca ccaccgcgcc cacccgggcg gcgctggcct
      661 gcatcaggcc tgcggggacc gcccccacca gcgtggagga gagcggcgcg gcaaggcaga
      721 ccgcccccgc aaagcaggag accgcggtga aaggctcttt ggtgagcata cccgcgatca
      781 ccgcgcaggc gataccgccc gccaggcccg ccaggcccag ccatttcgcc accctgtcgc
      841 tgcggtgctc ggaaaagctc tgccgcagaa agccgcgcac cagcgcggtg ggacggctca
      901 ccagcagatg ggggtccggc tcgcccaggc cgtcggtgac ccttttcacc agctcctcat
      961 ccgccacctg ataggcggcg gcgtggtcga agccggagct cgccatctca aagttttccg
     1021 ccaccatgcg ggcggtgatg cacttgccga acgtgttgcc ggcaaggcag agcaccgcca
     1081 ccccggcaaa gacggtgtaa tttgaggcgc cgaacttttc cgccccggtg agaaagacgc
     1141 cgcactgcac cagcgccgcc accgaggcca gcgcgggcag cagatcgggg ctgggggagg
     1201 aaaaaatcga ggacaggccg caggcgatgg tgttgtagtt gaccaccatg gccaccagca
     1261 gcagcacgat gttggcgatg agatagaaca gcggctgccc cgccgg
//
LOCUS       DBMDYA010000761         1304 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19192_length_1304_cov_1.411471, whole genome shotgun sequence.
ACCESSION   DBMDYA010000761 DBMDYA010000000
VERSION     DBMDYA010000761.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19192_length_1304_cov_1.411471"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>437)
                     /locus_tag="ACZPFK_13955"
     CDS             complement(<1..>437)
                     /locus_tag="ACZPFK_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1407459.1"
                     /translation="AGEVPDKKYTRSVLAILFPILYCLIDGLGTFSDSFVLDVVMDEV
                     TANLSYEFTFLLMAVFAFCWLTFVKKERFHPLQEKTKFAAALCETAGQFSYVFALSGN
                     AIVAAPTIASYSIFSALWARLFLKEKLTKAQYAVIALVMVGIA"
     assembly_gap    438..447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<448..878)
                     /locus_tag="ACZPFK_13960"
     CDS             complement(<448..878)
                     /locus_tag="ACZPFK_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1407460.1"
                     /translation="MWFIFTLVTILFWSGSDLFSNMGSAPDDKNSHWKMVLAVGAVMG
                     LHAIWQVTIGGVAYDPINMLKYLPVSAMYILSMIFGYAGLRYIELSVSSPICNSSGAI
                     TALLCFLFLGQRMTGLQLGAVLLISAGILALAVLEKRQADA"
BASE COUNT          300 a          354 c          412 g          228 t
ORIGIN      
        1 tggcgatgcc caccatcacc agcgcgatca cggcatattg ggctttggtg agcttctctt
       61 tcaaaaacag ccgggcccac agggcggaaa aaatgctgta agaggcgatg gtgggcgccg
      121 ccacgatggc gttgcccgac agcgcgaaca catagctgaa ctggcccgcg gtctcacaga
      181 gggcggcggc gaatttggtt ttctcctgca ggggatggaa ccgctccttt ttgacaaagg
      241 tcagccagca gaacgcaaac accgccatga gcagaaaggt gaactcgtag gagaggtttg
      301 ccgtgacctc gtccatcacc acatccagca caaaggagtc ggaaaaggtg cccagcccgt
      361 cgatcaggca gtagaggata gggaagagga tggccagaac gctgcgggta tattttttgt
      421 ccggcacctc ccccgcgnnn nnnnnnntgg gcgtcggcct gtcgtttttc cagcacggcc
      481 agcgccagga tgccggcgct gatcagcagc acggccccca gctgcaggcc ggtcatccgc
      541 tgccctaaaa acagaaagca gagcagcgcc gtgatggccc cggaggagtt gcagatgggc
      601 gaggagaccg acagctcgat ataccgcaga cccgcatagc cgaagatcat cgacaggata
      661 tacatggccg agacgggcag gtatttcagc atattgatcg ggtcataggc gacgccgccg
      721 atcgtgacct gccagatggc gtgcaggccc atcaccgcgc ccacggccag caccatcttc
      781 cagtgactgt ttttgtcgtc gggggcggag cccatattcg agaacaggtc cgagccgctc
      841 cagaacaaga tcgtgacaag ggtgaatata aaccacatat taaatttttg ctcccatcgc
      901 gcggcacagc cgcgtcattt tattttgtta ccggcggatg ggggacgccg gaggcggccg
      961 cacagggcgg cggtgccagg gatcgatcat aaaggttctc ctgctctggc ggcggtggag
     1021 ggcaccgcgg ctctgtgaat tttttgacga ttcatcatat cacattttga gggagggcac
     1081 aagagagcgg gtggcaccgg gataaaagag accccggtcg gaatccgccg ataaggcggt
     1141 tccggccggg gccaagagag aaagaacaaa ccaaaaagga aacagctttt aaaaaaatgg
     1201 aaagatcggg atcagcgtcg gctttgtccg tagtaggcgg cggggccgtg cttgcgcaaa
     1261 aagtgcttgt cctgcacccg ctggggcgcc ggggcggcgc tggg
//
LOCUS       DBMDYA010000762         1303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19214_length_1303_cov_1.162230, whole genome shotgun sequence.
ACCESSION   DBMDYA010000762 DBMDYA010000000
VERSION     DBMDYA010000762.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19214_length_1303_cov_1.162230"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="ACZPFK_13965"
     CDS             <1..442
                     /locus_tag="ACZPFK_13965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1407461.1"
                     /translation="LDLGAEAADVLLMVGQRAAGEGIRLEFTEPEQPCPVMADKNRLR
                     QVFLNVLDNALKYSPAGGTIRVGLLRQDGFAVLEVQDEGPGIEPEDLAHIKTKFYKGR
                     GAVRGSGIGLAVVDEIMTAHGGSLEIESAPGAGTLVRLRLPAKR"
     gene            498..>1303
                     /locus_tag="ACZPFK_13970"
     CDS             498..>1303
                     /locus_tag="ACZPFK_13970"
                     /inference="COORDINATES: protein motif:HMM:NF018798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1407462.1"
                     /translation="MSRHNLKEKFKTSVGGQALIEGVMMRGPSKLAIAVRDPAGEIQV
                     ETNDVKPNPVARIPILRGVVSFIDSLLVGYKAIMRSAEISMTEEEKEEEQSGFDRWLE
                     EHLGEKGMGVLMTVSALAGGLLAIVLFMVLPTFFTGLLGRFVALGAFRAVVEGAVKLG
                     LFVLYLFLISRMKEINRLFRYHGAEHKTIATYEAGEPLTVENVRGNSRFHPRCGTSFL
                     LLVLIISILVFSFVPWHSTLGRVGLKLLLLPLVMGLSYELIKFAGRHDNPV"
BASE COUNT          231 a          364 c          473 g          235 t
ORIGIN      
        1 gctggacctg ggggccgagg ccgccgacgt gctgctgatg gtgggccaga gggcggcggg
       61 ggaggggatc cggctggaat tcaccgagcc ggagcagccc tgcccggtga tggcggacaa
      121 aaaccgcctg cggcaggtct tcctcaatgt gctggacaac gcgctgaaat actcccctgc
      181 cggggggacg atccgggtgg ggctgctccg gcaggacggc tttgccgtgc tggaggtgca
      241 ggacgagggg ccgggtatcg agccggagga tctggcccac atcaagacca agttttacaa
      301 gggcaggggg gcggtgcgcg gcagcggcat cggcctggcg gtggtggatg agatcatgac
      361 ggcccacggc ggcagcctgg agatcgagag cgcgccgggc gcgggcacac tggtgcgcct
      421 ccgcctgccc gcaaagcggt agaacgcctt tatttcatat cacacagggg gcgcggctcc
      481 ccccgaaagg aaaatccatg agcagacaca atctcaaaga aaaattcaag acctcggtgg
      541 ggggacaggc cctcatcgag ggggttatga tgcgcgggcc cagcaagctg gcgattgcgg
      601 tgcgcgaccc ggcgggggag atccaggtgg agaccaacga cgtcaagccg aaccctgtgg
      661 cgcgaatccc catcctgcgc ggcgtggtca gcttcatcga ctcgctgctg gtgggctata
      721 aggccattat gcgctcggct gagatcagca tgaccgagga ggaaaaagag gaggagcaga
      781 gcgggtttga ccgctggctg gaagagcatt tgggcgaaaa ggggatgggg gtgctgatga
      841 cggtcagcgc cctcgcgggc ggcctgctgg ccattgtgct gttcatggtg ctgcccacct
      901 ttttcaccgg gcttctcggc cgctttgtgg cgctgggcgc gttcagggcg gtggtggagg
      961 gggcggtaaa gctggggctc tttgtgctct acctcttttt gatctcgcgc atgaaagaga
     1021 tcaaccgcct gttccgctat cacggcgccg agcacaagac catcgccacc tatgaggcgg
     1081 gcgagccgct cacggtggag aatgtacggg gcaattcccg ctttcacccg cgctgcggca
     1141 ccagcttttt gctgctggtg ctcatcatca gcatcctggt gttcagcttt gtgccctggc
     1201 attccacgct ggggcgggtg gggctgaagc tgttgctgct gccgctggtg atggggctca
     1261 gctatgagct gatcaagttt gcgggccgcc acgacaaccc ggt
//
LOCUS       DBMDYA010000763         1296 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19334_length_1296_cov_0.936402, whole genome shotgun sequence.
ACCESSION   DBMDYA010000763 DBMDYA010000000
VERSION     DBMDYA010000763.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19334_length_1296_cov_0.936402"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="ACZPFK_13975"
     CDS             complement(<1..790)
                     /locus_tag="ACZPFK_13975"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR1407463.1"
                     /translation="MEPKKIAVLFGGCSSEHEISLQSAAAVAAALDPALYEPVLTGIT
                     RDGRWLRYRGPAAAIADGSWERGDCTPALISPDRELHGLLEFAGGRALPERLDAAFPV
                     LHGKNGEDGTVQGLLELAGIPCVGCGVLASALCMDKDAAHRLARAAGVEVPPCAVLHA
                     PPRPEVLAACTHGLRYPLFVKPACAGSSFGVTRVETPDALPAAVAEAFRHDGKVLVEQ
                     AVPGFEVGCAVLGAGEALTVGEVDEIELAHGFFDYTEKYTLETAR"
BASE COUNT          236 a          420 c          458 g          182 t
ORIGIN      
        1 tgcgcgcggt ctccagcgta tatttttccg tataatcaaa aaagccatgg gccagctcaa
       61 tctcatccac ctctcccacg gtgagcgcct ctccggcgcc cagcacggca cagcccacct
      121 caaagcccgg caccgcctgc tccaccagca ccttgccgtc gtggcggaac gcttcggcca
      181 cagccgcggg cagcgcgtcc ggcgtctcca cgcgggtcac gccgaaggac gaacccgcac
      241 aggcgggctt gacgaatagc gggtaccgca gcccgtgggt gcatgccgcc agcacctcgg
      301 ggcgcggcgg agcatgcagc accgcacagg gcggcacctc cacccccgcg gcccgtgcca
      361 agcggtgcgc tgcgtcctta tccatacaca gggcgcttgc cagcacgccg cagcccacgc
      421 aggggatgcc cgccagctcc agcaggccct gcacggtgcc gtcctcaccg tttttgccgt
      481 gcagcaccgg gaaagcggcg tccagccgtt cgggcagcgc acggccgccc gcgaactcca
      541 gcaggccgtg cagctcccgg tcgggggaga tcagggcggg cgtgcagtcg ccgcgctccc
      601 agctgccgtc ggcgatggcg gcggcaggcc cgcggtagcg cagccagcgg ccgtcgcggg
      661 tgatgccggt caacaccggc tcgtagaggg cggggtccag cgccgcggcc acggcggcgg
      721 cggattgcag cgagatctcg tgctcgctgg agcagccgcc gaacagaaca gcgatttttt
      781 tgggttccat gagggtcagc ctctttcgac acagattccg cacggggcgc cgcgcaaaag
      841 agcttcgcgg cggcagcgct gctgcgcttt ttccgttttc ggtaccctca gtttacccca
      901 tttatctttc aaaacctttt agaaaggctg aaccctggct taaggttttc ttagcaaaag
      961 cttaagcctg cgctctcgcc tgaaaaaggc gcggggcgcg gcgataaggg tggcggggcc
     1021 tgagcggggc ggcgccgaaa gaggtggagc tgggcggaca ggttgggctg agaggagcgt
     1081 gcaagacggg ggaaagcggc tggaaaagat gaggctcagc agagggccgg tgcgcgggcg
     1141 gtcgcgggga gggcggcaag gcctgggaga agcacctgga aaagacggga cccagcagtg
     1201 gccggatcgc gggagaaaag cgccgcacgg gcggccaggc tgcggagaga tcggcaaggc
     1261 ccggagaaag cggctggaaa agacaggact cggcgt
//
LOCUS       DBMDYA010000764         1295 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19351_length_1295_cov_1.161642, whole genome shotgun sequence.
ACCESSION   DBMDYA010000764 DBMDYA010000000
VERSION     DBMDYA010000764.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19351_length_1295_cov_1.161642"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(49..507)
                     /gene="nusB"
                     /locus_tag="ACZPFK_13980"
     CDS             complement(49..507)
                     /gene="nusB"
                     /locus_tag="ACZPFK_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1407464.1"
                     /translation="MKRRTARENAFVAAFEESFRPGSMEEIIAYSREAGELEVDAFGE
                     TLLTGLVLHREEADKLIEPKLKNWKMNRIPRVCLIALELAVTEMFFGGEEDLDSVIIN
                     EAVELVKKFGGDEDYQFVNGVLGSLARERSGAAEEPGSGEAAPQGEEPRA"
     gene            complement(642..1022)
                     /locus_tag="ACZPFK_13985"
     CDS             complement(642..1022)
                     /locus_tag="ACZPFK_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1407465.1"
                     /translation="MEVKNSDMVGGSLQISTDVIAKISRLAALEIDGVKAVSCGNSPV
                     KGMFAKVSIQRPVTVELTDEVAEITVDVVVRYGYRIPSVSEALQKNVKASVQNMTGIT
                     VSKVNVVVTGVSLQPTDEDAAEEN"
BASE COUNT          213 a          466 c          358 g          258 t
ORIGIN      
        1 ccgccaggga ggtggcgtaa ttgctggtgt cgatgcccag aaatttcctc acgcccgcgg
       61 ctcctccccc tgcggggcgg cctccccact ccccggctct tccgccgcgc cggagcgctc
      121 tctcgccaaa gaacccaaaa cgccgttgac aaactgataa tcctcgtcgc cgccgaactt
      181 tttgaccagc tcgaccgcct cgttgatgat cacgctgtcc aggtcctcct cgccgccgaa
      241 aaacatctcg gtcaccgcca gctccagcgc gatcaggcag acccgcggga tgcggttcat
      301 cttccagttt ttcagcttcg gctcgatcag cttgtccgct tcttcccggt gcaggaccag
      361 gcctgtgagc agcgtctcgc cgaacgcatc gacctccagc tcgcccgcct cgcgggaata
      421 ggcgatgatc tcctccatgc tgccgggccg gaaggactcc tcaaacgcgg cgacaaaagc
      481 gttctctctg gctgtacgtc ttttcatcgg ataccccttt tgcacaaatt tgccctcctg
      541 ccggtgcggg agagccgaaa gatgggcgtc cccggccgcg gcagagggcc cgcaaagccg
      601 gagacacgaa aaatagccgc cgcgcggcta ttttttgtgg ttcaattttc ttccgccgca
      661 tcctcgtcgg tgggctgcag cgagacgccg gtgaccacga cgttgacctt ggagaccgtg
      721 atgccggtca tgttctgcac cgacgctttc acgttcttct gcagggcctc gctgaccgag
      781 gggatgcggt acccgtagcg gaccaccacg tccaccgtga tctcagccac ctcgtcggtc
      841 agctccaccg tcaccggcct ttggatgctg accttggcga acatgccctt taccggcgag
      901 ttgccgcagg agaccgcctt gaccccatcg atctccagcg cagcaagacg ggagatcttg
      961 gcgatgacgt cggtggagat ctgcaggctg ccgcctacca tatcagaatt tttcacttcc
     1021 atgaaagtgc ctccttttat ctgcagccgt ctgtctttca cagcggggac accccatctg
     1081 caaaaaacgc ggcccggccg atcagtattt tctatagccg cgccggccca gccggcaggc
     1141 gtttgggatc tctttgcaag cgcccggccc gccggcagct gccggcgcaa gaccccccgc
     1201 cgcgccggcc cgtggccgtg cggccactgc tgctttttgc ggaaaagccc cccgccggtc
     1261 ggcccgcccc ggcccctgcg gcctctccgc ccgct
//
LOCUS       DBMDYA010000765         1293 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19395_length_1293_cov_1.219799, whole genome shotgun sequence.
ACCESSION   DBMDYA010000765 DBMDYA010000000
VERSION     DBMDYA010000765.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19395_length_1293_cov_1.219799"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(124..>1293)
                     /locus_tag="ACZPFK_13990"
     CDS             complement(124..>1293)
                     /locus_tag="ACZPFK_13990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1407466.1"
                     /translation="RETATAFALLPIVLERGYAGCPDMTQLSRRLASLYGAGLSADTS
                     VQGENRVVSISICGLRDRYALHGEALSAAYADILFGAAFDPYLVDGAFDPQAVKIEKQ
                     KLRELLEGEINNKRAYCVQQARRKFFGDSPAGIERNGYLEDLDRVTPQALAEAYAQMV
                     RTARVEVLALGADADEVEARLLQKLDAQRRVPALILPAMAMPAGAARSYTEQVDAVQG
                     KLCLLFTAGRPLTGEELTHMRLAAALFGGLPSSRLFRNVRERQSLCYYCSCGFSYLTS
                     ALSVDSGVQPENAAKAQKAILRELEALAAGPITEQELRETKLSYLGALRAVGDSLASI
                     EMWAFREILRGTFQDPQEVERAVERTTADEIRSALSLFTLSVSYLLTKGGAADGR"
BASE COUNT          229 a          467 c          395 g          202 t
ORIGIN      
        1 caggcctgca cggcgcgata gccctccatg ggggccacaa agacggtgag gccgttttcc
       61 agcgtcagct tctcatagct gaaatccacc cggccggagc gcagcgtctc cgaccgcaaa
      121 atcttaccgc ccatccgccg cgcccccttt cgtcaacagg taggacaccg acagcgtgaa
      181 gagagacagc gcgctgcgga tctcatcggc ggtggtgcgc tccaccgcgc gttccacctc
      241 ctgcgggtcc tgaaaggtgc cgcgcaaaat ctcccggaaa gcccacatct cgatggaggc
      301 caggctgtcc cccaccgcgc gcagcgcgcc cagataagag agcttcgtct cccgcagctc
      361 ctgctcggta atggggccgg cggccagcgc ctccagctcg cgcaggatag ctttttgggc
      421 ctttgccgca ttttccggct gcacgccgct gtccacgctg agggcagagg tgagataaga
      481 aaagccgcag gagcaataat agcacaagct ctggcgctcc cgcacattgc ggaacagcct
      541 tgaggacggc aggccgccga acaaagccgc cgccaaccgc atgtgggtga gttcctcccc
      601 cgtgaggggc cggccggcgg taaacagcag gcagagcttg ccctgcaccg cgtccacctg
      661 ctcggtatag ctgcgcgccg cgcccgcagg catcgccatg gcgggcagga tcagcgccgg
      721 gacccggcgc tgcgcgtcca gcttttgcag cagccgcgcc tccacctcgt ccgcgtccgc
      781 ccccagcgcc agcacctcca cccgggccgt gcgcaccatc tgtgcataag cctcggccag
      841 cgcctgcggc gtgaccctgt ccaggtcttc cagatagccg ttgcgctcaa tgcccgccgg
      901 ggaatcgccg aaaaatttgc gccgcgcctg ctgcacacag taagcgcgct tgttgttgat
      961 ctcgccctcc agcagctcgc gcagcttttg tttttcgatc ttcaccgcct gcgggtcaaa
     1021 ggccccgtcc accagatagg ggtcaaaggc ggcgccgaag aggatatccg cataggccgc
     1081 cgacagcgcc tcgccgtgca gggcgtagcg gtcacgcagg ccgcagatgc tgatgctcac
     1141 cacccggttt tcgccctgca ccgaggtgtc ggccgacaga cccgcaccgt aaagcgaggc
     1201 cagcctccgc gagagctggg tcatatccgg gcagcccgcg tagccccgct ccagcacgat
     1261 gggcagcagc gcaaaggcgg tggccgtctc gcg
//
LOCUS       DBMDYA010000766         1289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19482_length_1289_cov_1.063973, whole genome shotgun sequence.
ACCESSION   DBMDYA010000766 DBMDYA010000000
VERSION     DBMDYA010000766.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19482_length_1289_cov_1.063973"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="ACZPFK_13995"
     CDS             <1..597
                     /locus_tag="ACZPFK_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1407467.1"
                     /translation="CKLVFPTVLGQLLVGAPAAWAFSRFRFRGRRLLFTVYIVLMLMP
                     FQVTMVPSYLVANGLGLMDTLWAVILPGIFSTFPVFIMAKGFDAVPDALLEAAALDGA
                     GPLQTFFRIGLPLGVPGILSALVLGFLECWNAIEQPMTFLKTKSLWPLSLYLPEIAAE
                     KLGLAMVSSLLMLAPSVLIFLFGQKYLELGIQSSGLKE"
     gene            605..>1289
                     /locus_tag="ACZPFK_14000"
     CDS             605..>1289
                     /locus_tag="ACZPFK_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407468.1"
                     /translation="MKWLELKEKTLALAGRLPRPAEAGQRRALGNILRFFALMLAFTL
                     VARGAAAATLPSVSLVRPAPGEIVQDLQTGGAVQAADPLGVEAPAGLTVKKWLAQPGQ
                     KIKAGDPLLQLDADEAAEALARAGLELEQLRLDLQKLLDGVSVDSGALSGAATALERA
                     KADAQGQREAAQKAVAEAQAALDAAAGEASARAAALEALRAQQDPAASAEELAAAAAA
                     LDEANARRDQ"
BASE COUNT          187 a          404 c          486 g          212 t
ORIGIN      
        1 tgtaagctgg tattccccac ggtgctgggc cagctgctgg tgggcgcgcc ggcggcctgg
       61 gcgttctcgc ggtttcggtt ccggggccgc aggctgctct ttaccgttta tattgtgctc
      121 atgctgatgc cctttcaggt gacgatggtg cccagctatc tggtggccaa cggcctgggc
      181 ctgatggaca ccctctgggc ggtgatcctg cccggcatat tttcaacctt tccggtcttt
      241 atcatggcaa agggctttga cgcggtgccc gacgcgctgc tggaggcggc ggcgctggac
      301 ggcgcgggcc cgctgcagac cttttttcgc atcgggctgc cgctgggcgt gccgggcatc
      361 ctctcggcgc tggtgctggg ctttctcgag tgctggaacg ccatcgagca gcccatgacc
      421 tttttgaaga ccaaatccct ctggccgctt tccctctatc tgcccgagat cgcggcggaa
      481 aagctgggcc tggcgatggt gtccagcctg ctgatgctgg ccccgtcggt gctcatcttc
      541 ctgttcgggc aaaaatacct cgagctcggc atccagtcga gcggactcaa ggagtgaccc
      601 ccgtatgaaa tggcttgaac tcaaagaaaa aacgctggcg ctggccgggc ggctgccccg
      661 cccggcggag gcgggccagc ggcgggcgct gggcaatatt ctgcgcttct ttgcgctgat
      721 gctggcgttc accctggtgg cccggggcgc ggcggcggcc accctgccca gcgtgagcct
      781 ggtgcgcccc gcgccggggg agatcgtaca ggacctgcag acagggggcg cggtgcaggc
      841 cgcggacccg ctgggcgtgg aggcccccgc cgggctgacg gtaaaaaaat ggctggctca
      901 gcccggtcag aaaataaagg cgggggaccc gctgctgcag ctggatgcgg acgaggcagc
      961 cgaggcgctg gccagggccg ggctggagct ggagcagctg cggctcgacc tgcaaaagct
     1021 gctggacggc gtgagcgtgg acagcggcgc cctctccggt gcggccacgg cgttggagag
     1081 ggccaaggcg gacgcgcagg ggcagcggga ggcggcccaa aaggccgtgg ccgaggcaca
     1141 ggcggcgctg gatgcggccg cgggggaggc atcggccagg gcggcggcgc tggaggccct
     1201 gcgcgcccag caggaccccg ccgcgagcgc ggaagagctg gccgccgccg cggcggcgct
     1261 ggacgaggcg aacgcccgca gggatcagg
//
LOCUS       DBMDYA010000767         1281 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19632_length_1281_cov_1.000847, whole genome shotgun sequence.
ACCESSION   DBMDYA010000767 DBMDYA010000000
VERSION     DBMDYA010000767.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19632_length_1281_cov_1.000847"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="ACZPFK_14005"
     CDS             <1..458
                     /locus_tag="ACZPFK_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1407469.1"
                     /translation="GIAQALLHDPEVLILDEPTAGLDPEERVRLRALLGELAEQKTVL
                     LSTHVVGDIEASCEEIAVMDGGKLIYHGAVEALRQRAQGKVYETALAHGALAAFRAHH
                     QVTSVTGSGKNCLVRFLAPQEDPCIGRPCEPSVEDAYLLCVHAAGGEAR"
     gene            455..>1281
                     /locus_tag="ACZPFK_14010"
     CDS             455..>1281
                     /locus_tag="ACZPFK_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407470.1"
                     /translation="MKLFLAECRRLLHSAVYWLTVLALVFFLFSQGVLPDDKRIEEPQ
                     PGEEQSVLVPSGDPALIMPEAVSALYEGFLSNSFSTYPHGLIKHVKLTEAKRSRLAEL
                     LAQLTGLDAETLLAAVEGEEDQGGAFTVGGDEMQVNPDGSISITGGGAAAAQAGGARP
                     AAVPLRPGLEWEEFTGLMEQADRLLGGGSDWAVDSLSGYGRVPTTYEALMEEYRAGLA
                     HDRYTGAHARLFCDYAVCMIALLPAFTATAFFWQDRRRVAPMLYTRRVSGAALIGAR"
BASE COUNT          193 a          392 c          476 g          220 t
ORIGIN      
        1 tgggcatcgc ccaggcgctg ctgcacgacc cggaggtatt gatcctcgac gagcccaccg
       61 ccgggctgga cccggaggag cgggtgcggc tgcgagccct gctgggcgag ctggcggagc
      121 agaagacggt gctgctctcc acccatgtgg tgggggatat cgaggcatcc tgtgaggaaa
      181 tagctgtgat ggacggcggc aaactgatct atcacggcgc tgtggaggcg ctgcggcagc
      241 gcgcgcaggg aaaggtctat gagacggcgc tggcccacgg ggcgctggcg gcgttccggg
      301 cgcatcacca ggtcacctcg gtgaccggca gcggaaaaaa ctgcctggtg cgctttctcg
      361 cgccgcagga ggacccctgc atcgggcggc cctgtgagcc gagcgtggag gacgcctatc
      421 tgctctgcgt ccacgcggcg ggaggtgagg cgcgatgaag ctgtttttgg cagagtgcag
      481 gcggctgctc cacagcgcgg tctattggct gaccgtgctg gcattggtgt tctttttgtt
      541 cagccaggga gtcctgcccg acgacaagag gatcgaggag ccgcagccgg gggaggaaca
      601 gtcggtgctg gtgccctccg gcgacccggc gctgatcatg ccggaggcgg tgtcggcgct
      661 ttatgagggc tttttgtcca acagcttttc cacctatccc cacggcctca tcaagcatgt
      721 gaagctgacc gaggcaaagc ggagccgatt ggcggagctt ctcgcccagc tcaccgggct
      781 ggatgcagaa acgctgttgg cggctgtgga gggggaagag gaccaaggcg gcgcgttcac
      841 ggtcggcggg gatgagatgc aggtgaaccc ggacggcagc atttccatca ccggcggggg
      901 cgcggccgcc gcgcaggcgg gcggggcccg ccccgccgct gtgccgcttc gccccgggct
      961 ggagtgggag gagttcaccg gcctgatgga gcaggccgac cggctgctgg gcggcggctc
     1021 cgattgggcg gtagacagcc tttccggcta cggacgggtg cccaccacct acgaggccct
     1081 gatggaggaa taccgggccg gtctcgccca cgaccgctat accggcgccc atgcccgcct
     1141 gttctgtgat tatgcggtgt gcatgatcgc gcttttgccc gcctttaccg ccaccgcgtt
     1201 tttctggcag gaccggcggc gggtggcgcc gatgctgtac acccgccggg tctcgggggc
     1261 tgccctcatc ggcgcgcgct t
//
LOCUS       DBMDYA010000768         1276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19698_length_1276_cov_1.579574, whole genome shotgun sequence.
ACCESSION   DBMDYA010000768 DBMDYA010000000
VERSION     DBMDYA010000768.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19698_length_1276_cov_1.579574"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="ACZPFK_14015"
     CDS             complement(<1..415)
                     /locus_tag="ACZPFK_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407471.1"
                     /translation="MEKETGLKKGFQEAGFPLKALGTLAGMLLIGFAMAGMYLGDLGA
                     DPISILMEGVSRAAGLSKGMGTNLVNLAFFLFALLFNRRSIRWATLISVLAMGPSVDL
                     GLWALGALLPAPLPFPARLLLGAASCALLGFAIGFY"
     gene            complement(423..>1276)
                     /locus_tag="ACZPFK_14020"
     CDS             complement(423..>1276)
                     /locus_tag="ACZPFK_14020"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR1407472.1"
                     /translation="DHFVRGGETIPSLSMELFCGGKGLNQSVALARAGGEVYHAGKIG
                     ADGEMLLDRLRENGVNVDYVGVSDSLPTGHAVIQVDAKGQNCIIVCAGANGSLTEEEM
                     DGVLSHFGQGDILLLQNEINGIGPLLRKAYGRGMQIALNPSPIDGELAALEELRYVTW
                     FILNEVEGAELTGKTEGEEICAALLKKYPRARVVLTLGKKGCLYRDGGQTAAHGIYDV
                     PVVDTTAAGDTFTGYFLAGVTDGLPVEQILELASKASSLAVSRKGASDSIPARAEVEA
                     CDLAPLG"
BASE COUNT          221 a          471 c          387 g          197 t
ORIGIN      
        1 gataaaagcc gatggcaaag cccagcagcg cgcaggaggc cgcccccagc agcagccgcg
       61 ccggaaaggg cagcggcgcg ggcagcaggg cgcccagcgc ccacaggccc agatcgacgc
      121 tggggcccat ggcgagcacc gagatcaggg tggcccagcg gatgctgcgg cggttgaaca
      181 gcagggcgaa aagaaagaac gccaggttca ccagattggt ccccatcccc ttgctcagcc
      241 cggcggcccg cgagaccccc tccatcaaaa tcgagatggg gtcggcgccc agatcgccca
      301 ggtacatccc cgccatagca aagccgatca gcagcatacc ggccaaggtg cccagcgctt
      361 tcagcggaaa gcccgcctcc tgaaagcctt ttttcaggcc cgtttccttt tccatcgccc
      421 gctcacccca ggggggccaa gtcacaggcc tccacctcgg cccgcgccgg gatggaatcc
      481 gacgcgccct tgcggctgac cgccagcgag gaggccttgc tggccagctc cagaatctgc
      541 tccaccggca gcccgtcggt gaccccggcc aaaaaatagc cggtaaaggt gtcgcccgcc
      601 gcggtggtgt ccaccaccgg cacatcgtag atgccgtggg cggcggtctg cccgccgtcc
      661 cggtagaggc agcccttttt gcccagcgtg agcaccaccc gcgcgcgcgg atactttttg
      721 agcagggcgg cgcagatctc ctctccctcc gttttgccgg tgagctcggc gccctccacc
      781 tcgttgagga tgaaccaggt cacatagcgc agctcctcca gcgcggccag ctccccgtcg
      841 atgggcgagg ggttgagcgc gatctgcatg ccgcgcccat aggccttgcg cagaagcggg
      901 ccgatgccgt tgatctcgtt ctgcagcagc aggatatccc cctgcccgaa gtgggagagc
      961 accccgtcca tctcctcctc tgtgagcgat ccgttcgccc cggcgcagac gatgatgcag
     1021 ttctgcccct ttgcgtccac ctggatgacc gcatggccgg tgggcaggct gtcgctgacg
     1081 cccacataat ccacgttgac gccgttttcc cgcaggcggt ccagcagcat ctccccgtcc
     1141 gcgccgatct tgcccgcgtg gtacacctcg cccccggcgc gcgccagcgc caccgattgg
     1201 ttcagcccct tgccgccgca gaacagctcc atgctcagcg aggggatcgt ctccccgccc
     1261 cgcacaaagt ggtcga
//
LOCUS       DBMDYA010000769         1276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19700_length_1276_cov_1.347234, whole genome shotgun sequence.
ACCESSION   DBMDYA010000769 DBMDYA010000000
VERSION     DBMDYA010000769.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19700_length_1276_cov_1.347234"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="ACZPFK_14025"
     CDS             <1..682
                     /locus_tag="ACZPFK_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1407473.1"
                     /translation="ARKDVRFDRANILRMLRLGAPIALQELLVSISFLVILAIVNSLG
                     VIASAGVGVAEKLCAFIMMVPSAYMQSLSAFVGQNIGAGQPGRAKRAMLYGMAAAFAF
                     SAVMAALAFFKGDLLAGIFAKDPQVVAAAWDYLRAYAIDTLLVSFLFCFCGYFNGSGK
                     TTFVMVQGIVGAFLVRIPVSYFMGTLRPVSLFKVGLATPASTLVQILLCVVYFIWVER
                     QVRRQRRL"
     gene            complement(830..>1276)
                     /locus_tag="ACZPFK_14030"
     CDS             complement(830..>1276)
                     /locus_tag="ACZPFK_14030"
                     /inference="COORDINATES: protein motif:HMM:NF019359.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HIR1407474.1"
                     /translation="MRQSILQEFGSIEQWRAHYIERASKEDMQRGYQKMVEWYGGKNA
                     ALKAMTDPPSMEIAQACLKRVDAVLQKLIAKKGEPVSSFAVKEIVGEYGYIMKQLYQI
                     KREEGLMLAIASSYEDERIRTGTDEKYGEGAAEFFKRAIETFYEKE"
BASE COUNT          191 a          380 c          395 g          310 t
ORIGIN      
        1 tgcgcgcaag gacgtacgct ttgaccgggc caatatcctg cggatgctgc ggctgggcgc
       61 gcccatcgcg ctgcaggagc tgctggtcag catctccttt ctcgtgattt tggccatcgt
      121 caactcgctg ggggtcatcg cctcggcggg cgtaggggtg gcggaaaagc tctgcgcgtt
      181 catcatgatg gtgccctcgg cctatatgca gtcgctgtcc gcctttgtgg ggcagaacat
      241 cggcgcgggg cagccggggc gggcaaagcg ggccatgctg tacggtatgg cggcggcgtt
      301 cgctttcagt gccgtcatgg cggcgctggc gtttttcaaa ggggacctgc tggcgggtat
      361 ctttgccaag gacccgcagg tggtcgcggc cgcgtgggat tatctgcgcg cctacgccat
      421 cgacacgctg ctggtctcgt tcctgttctg cttctgcgga tatttcaacg gcagcggcaa
      481 gaccaccttt gtcatggtgc agggcattgt gggcgcgttt ctggtgcgca tcccggtctc
      541 ttattttatg gggacgctgc gcccggtctc gctgttcaag gtgggcctgg cgacgcccgc
      601 ctccacgctg gtgcagatct tgctctgcgt cgtctatttt atctgggtgg aaaggcaggt
      661 ccgcaggcag cggcggttgt gacgccgcag cccccggcgt tttccgcaag gggcgctcgg
      721 gctccaagac tgtgaaaaaa gtggagagaa gcgtttagcg cttctctcca ttttttgttt
      781 ataggccggt gtaccgggtt aaaaagacgg cgcgaccccg cggccgttgt cactcttttt
      841 cataaaaggt ctcgatagcc cgtttaaaaa actccgcggc gccctcgccg tatttttcgt
      901 ctgttccggt gcggatgcgc tcatcctcat agctggaggc gatggccagc atcagcccct
      961 cttcgcgctt gatctgatag agctgtttca tgatatagcc gtactcgccc acgatctctt
     1021 tcacagcaaa ggagctcacg ggctcgccct ttttggcgat cagcttttgc agcaccgcgt
     1081 ccacccgttt caggcaggcc tgggcgatct ccatgctggg cgggtcggtc atcgctttca
     1141 gggcggcgtt tttacccccg taccactcca ccatcttctg gtagccgcgc tgcatatcct
     1201 ccttggaggc gcgctcgata tagtgggccc gccactgctc gatgctgccg aactcctgca
     1261 aaatgctctg gcgcat
//
LOCUS       DBMDYA010000770         1270 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19816_length_1270_cov_1.336185, whole genome shotgun sequence.
ACCESSION   DBMDYA010000770 DBMDYA010000000
VERSION     DBMDYA010000770.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19816_length_1270_cov_1.336185"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="ACZPFK_14035"
     CDS             <1..884
                     /locus_tag="ACZPFK_14035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1407475.1"
                     /translation="VLFGKNLSSPQQLTAFLSAMQRASALPLLTGIDEEGGAVSRLAN
                     APGFDLPRYESMAAVGATGDEAAAKEVGAVIGGYLRQSGFTLDFAPVADVNTNPENLV
                     IGERAFGDDPQLVARMVGAEIEGLHEAGVLSCVKHFPGHGDTRGDTHDGYVSLDKTWD
                     ELKACELIPFVDNLSAADLVMVAHITLPNVTGDGLPASLSEELVTGRLRGELGYTGLI
                     VTDSLAMGAIAAEYSAAESAVLALKAGVDLLLMPEDYAAAYEGVLDAVESGALTEARI
                     DESVGRIVALKLKCGLL"
     gene            933..1235
                     /locus_tag="ACZPFK_14040"
     CDS             933..1235
                     /locus_tag="ACZPFK_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407476.1"
                     /translation="MKKALMRGGVALLGLLWGALSYGWLLFSLALAFVTSRPGSFEYE
                     YEDKSMIPLGWLGLGMYAAFLAVIVWRLHKGRKLALFLLPALLALCALVLWNVAGA"
BASE COUNT          196 a          366 c          471 g          237 t
ORIGIN      
        1 tggtgctgtt cggcaaaaat ctctcgtcgc cgcagcagct gacggctttt ctctcggcga
       61 tgcagcgggc cagcgcgctg ccgctgctga cggggatcga tgaggagggc ggcgcggtct
      121 cccgcctcgc aaacgccccg ggctttgatc tgccccggta tgagagcatg gcggccgtgg
      181 gcgccaccgg cgatgaggcg gccgccaagg aggtgggcgc ggtcatcggc ggctatctgc
      241 gccaatcggg ttttaccctg gattttgcgc cggtggcgga cgtcaacacc aacccggaga
      301 acctggtcat cggcgagcgc gctttcggcg acgacccgca gctggtggcg cggatggtgg
      361 gcgcggagat cgagggcctc cacgaggcgg gggtgttgag ctgcgtcaag cattttcccg
      421 gccacggcga cacccggggg gatacccacg acggctatgt atccctggat aagacctggg
      481 acgagctgaa agcctgtgag ttgatcccgt ttgtggataa tctctccgct gccgacctgg
      541 tgatggtggc ccacatcacc ctgccgaacg tcacgggcga cggcctgccc gcctcccttt
      601 ccgaagagct ggtcaccggc aggctgcggg gcgagctggg ctataccggc ttgatcgtca
      661 ccgattccct ggccatgggc gccatcgcgg cagagtactc cgccgccgaa agcgcggtgc
      721 tggccctcaa ggcgggggtg gacctgctgc tgatgccgga agattacgcc gcggcctatg
      781 agggggtgct ggacgcggtg gagagcggcg ccctcaccga ggcgcgcatc gacgagagcg
      841 tggggcgcat tgtggcgctc aagctgaagt gcgggctgct gtgagtggaa aagacgcccg
      901 cgcggataaa gaaaagggga agtgggactg ggatgaaaaa ggcgctgatg cgcggcgggg
      961 tcgccctgct ggggctgctc tggggcgcgt tgagctacgg ctggctgctg ttctcccttg
     1021 cgctggcttt cgtcaccagc cggccgggca gttttgaata tgagtatgag gataagagta
     1081 tgattccctt ggggtggctt gggctcggga tgtatgccgc ttttttggcc gtgattgtgt
     1141 ggaggctgca caaggggcga aagctcgccc tgttcctgct ccccgcgctg ctggccctct
     1201 gcgccctcgt cttgtggaat gtggctgggg catgaggagg taaaagcgga gcggggcggc
     1261 ccctgcgcgc
//
LOCUS       DBMDYA010000771         1264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_19928_length_1264_cov_1.315563, whole genome shotgun sequence.
ACCESSION   DBMDYA010000771 DBMDYA010000000
VERSION     DBMDYA010000771.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19928_length_1264_cov_1.315563"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            37..480
                     /locus_tag="ACZPFK_14045"
     CDS             37..480
                     /locus_tag="ACZPFK_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1407477.1"
                     /translation="MEDMLRQCIRKALNLTPLLRSTLIRPQEVSPVADLPSTQLQALF
                     VLNVTPPLSMSVLARKLAISKQQLTKIADALVARGCVSRSSDEKNRRVILLSLTEEGR
                     ALVRALQDEVVERMLPQFGRLDESELQEFSQAADTIRRLLDKVKN"
     gene            611..>877
                     /locus_tag="ACZPFK_14050"
     CDS             611..>877
                     /locus_tag="ACZPFK_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR1407478.1"
                     /translation="MTREETRRMAADLIRLAPIFSGAVLRPIGSCPGCPLTAGQLQAL
                     ICIQCQPGLNMTALAQRLGVSRQQLTRTVDALVAQGLVERITQAD"
     assembly_gap    878..887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1052..>1264
                     /locus_tag="ACZPFK_14055"
     CDS             1052..>1264
                     /locus_tag="ACZPFK_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018218.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR1407479.1"
                     /translation="MKETKNPKRPLIYYYLIAMLALLLINAFLAPMLQKSRVQEVSYD
                     TFLSMVSEGKVKEVEMDDSQIAFSPSD"
BASE COUNT          246 a          419 c          369 g          220 t
ORIGIN      
        1 gacatctccg ccccaggaaa gtgaagtttc gcccatatgg aagatatgct tcgtcagtgc
       61 atccgcaaag cgctcaacct caccccgctg ctgcgctcca ccctgattcg cccccaggag
      121 gtcagcccgg tggcggacct gccctccacc cagctgcagg cccttttcgt gctcaacgtc
      181 acgccgccgc tcagtatgag cgtgctggcc cgcaagcttg ccatctccaa acagcagctc
      241 accaagatcg ccgacgcgct ggtggcgcgc gggtgcgtgt cccgctccag cgacgagaaa
      301 aaccggcggg tgatcctgct ctccctcacc gaggaaggca gggccctggt gcgcgcgctg
      361 caggatgagg tcgtcgagcg gatgctgccc cagttcggcc gcctggacga gagcgagctg
      421 caagagtttt ctcaagccgc cgacaccatc cgccgcctgc tggacaaggt caaaaactga
      481 cgcttcgccc cgtttctccc cgctgccgcc cgcagcgggg agttttgatt ttcattaaat
      541 gttaacaaat gtgtcatatc cccgttttat tcctgcgcta tcctattgta catgcaaagg
      601 aggcgatgtc atgacccggg aggaaacacg ccgcatggcg gcggacctga tccggctggc
      661 gcccatcttc agcggcgcgg tgctgcgccc catcggcagc tgtccgggct gcccgctcac
      721 cgcgggccag ctgcaggcgc tcatctgcat ccagtgccag cccggcctca acatgaccgc
      781 cctggcccag cgcctggggg tgagccggca gcagctcacc cgcacggtgg acgccctggt
      841 ggcccagggg ctggtggagc gcatcaccca ggcggacnnn nnnnnnngcc tgcctgtggg
      901 agcagctggc cgacgccctg tcggaaagcc ttttcaacac cctcgaaagc ctttccggac
      961 aggagcagca ggcgctgacc ggggcctgcg agacgataac gaaaatcctg ctgcggcatc
     1021 tgccggccgg gagagaggaa gacgaaagcc aatgaaagag accaaaaacc ccaaaagacc
     1081 gctcatctat tactatctca tcgccatgct ggcgctgctg ctcatcaacg cctttcttgc
     1141 ccccatgctg cagaagagcc gggtgcagga ggtcagctac gacacttttt tgtccatggt
     1201 gagcgagggc aaggtaaaag aggtggagat ggacgacagc cagatcgctt tctcccccag
     1261 cgat
//
LOCUS       DBMDYA010000772         1250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20197_length_1250_cov_1.228895, whole genome shotgun sequence.
ACCESSION   DBMDYA010000772 DBMDYA010000000
VERSION     DBMDYA010000772.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20197_length_1250_cov_1.228895"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="ACZPFK_14060"
     CDS             complement(<1..454)
                     /locus_tag="ACZPFK_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407480.1"
                     /translation="MTALLLAYLKTGLLVSLAVVGLLLTLPLLDRRYTARWRCRVWVA
                     LAVLLLLPLPSLPGRAALRLTLPKTVAEPLAGPLFSALPGGGQNAAGEQASGAADAPT
                     VLDTAAGQEAAQQGPAQQEGGGHPPSGGLASSPQAGVQPPAAAQPQRDA"
     gene            complement(454..840)
                     /locus_tag="ACZPFK_14065"
     CDS             complement(454..840)
                     /locus_tag="ACZPFK_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1407481.1"
                     /translation="MQPILKRLPDAELEVMKALWDAGRPLTRPELDGALCGHRWAATT
                     VLGLLSRLEGKGFLTRRRQGRGYLYAPAVPREAYLAVEGRSALGRMFAGSAKNLVAAL
                     HECDALSRQEIDELSAYLEALKRGDA"
BASE COUNT          226 a          436 c          399 g          189 t
ORIGIN      
        1 cggcatccct ctgcggctgc gcggctgcgg gcggctgtac gccggcctgc ggggaggagg
       61 caagcccgcc ggagggggga tggccgccgc cctcctgctg cgcgggtccc tgctgtgcgg
      121 cctcctgccc tgctgcggtg tccaggacgg tcggtgcatc cgccgccccg gacgcttgct
      181 caccagcggc gttctgaccg ccgcccggca gcgcactgaa cagcggcccg gccaagggct
      241 ccgccacggt cttgggcagg gtcaggcgca gcgccgcccg gccggggagc gacggcaacg
      301 gcaacagcag cagcaccgcc agcgccaccc agacccggca gcgccagcgg gcggtatacc
      361 gccggtccag cagcggcagg gtcagcagca gccccaccac cgccagcgac accagcagcc
      421 cggttttcaa ataggcgaga agcagcgcgg tcatcacgca tccccccgtt tcagcgcctc
      481 cagataggcg gacagctcgt cgatctcctg gcggctcagc gcgtcacatt cgtgcagggc
      541 ggccaccagg tttttggcgc tgcccgcaaa catccgcccc agggccgagc gcccctccac
      601 cgccagatac gcctcccgcg gcacggccgg ggcgtagaga tagccccggc cctggcgccg
      661 ccgggtcaaa aagcccttgc cctccagccg cgagagcagc cccagcacgg tggtcgcggc
      721 ccagcggtgg ccgcacaggg cgccgtccag ctcggggcgg gtcagcgggc ggcccgcgtc
      781 ccaaagggct ttcatgacct ccagctccgc atccggcagc cgcttcagta tcggttgcat
      841 cccgtcacct cattccacaa atgtagaatt gaatctaatt taatactacg gttgtagaaa
      901 taagctgtca agagaaaaaa acagacaaaa tgcgcccgtt tttttcggca agcctcacaa
      961 aaatcagcga gcagctttgc cccgcggccg gccggccgct gtccgccgcc ggcgagaggg
     1021 gcgcgccgcc ccacggcggt tcccggcagc cggaaaaagc ggtgcatttt ggggatgcgg
     1081 ccatcgtcct cgttgctcca cggcggccca ggcggatgga aaagcagtgg actttgctcg
     1141 gcgcgcctgt tatgatgata aaaacatcag tcacaatctt tcaaaaggag ggtcaaagaa
     1201 tgagagcgga ttcagatcgc gtttctctat tttcaggatt ttgagcggtc
//
LOCUS       DBMDYA010000773         1238 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20427_length_1238_cov_1.737907, whole genome shotgun sequence.
ACCESSION   DBMDYA010000773 DBMDYA010000000
VERSION     DBMDYA010000773.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20427_length_1238_cov_1.737907"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..204)
                     /locus_tag="ACZPFK_14070"
     CDS             complement(31..204)
                     /locus_tag="ACZPFK_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR1407482.1"
                     /translation="MEVKNYTLAQMQPGLCESFTVTVTEEKMAQFCALSGDVSPIHMD
                     EAYAAGRGYPGRV"
     gene            complement(315..557)
                     /locus_tag="ACZPFK_14075"
     CDS             complement(315..557)
                     /locus_tag="ACZPFK_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1407483.1"
                     /translation="MSEQEIFDSVQEIFRTVFDDDGIVLTREMTADDIEDWDSLEQIN
                     LLVAIEKKFSIKFKLDDVKNLPNVGAMLDLVKRMVA"
     gene            complement(574..>1238)
                     /locus_tag="ACZPFK_14080"
     CDS             complement(574..>1238)
                     /locus_tag="ACZPFK_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR1407484.1"
                     /translation="PEGMIASLDRAGYFEVTTLSADDAKRNEMYRQNLARAAVQQSFG
                     SYDDYLRSLEMTAELGGFDQPHAERITQLINKTNQFNLTTRRYTAQEVEALIGSSAAV
                     TLYGRLTDKFGDNGLVSAMIGSVEGDVCTVDLWIMSCRVFKRNLEQAMMDEFVRRCAA
                     RGIKKIVGRYLPTAKNLLVKEIYGAMGFEKVSENDAETVFSLDVAAYQKQNDILAVTT
                     KE"
BASE COUNT          222 a          389 c          353 g          274 t
ORIGIN      
        1 ggagaaaaag gcggccacca gcataccgta tcagacccgg ccggggtagc cgcgcccggc
       61 ggcgtaggcc tcgtccatat ggatggggct cacgtcgccg gacagcgcgc agaactgcgc
      121 catcttttct tccgtgaccg tgacggtgaa gctctcgcac aggccgggct gcatctgggc
      181 gagcgtatag tttttgactt ccacaacaaa acctctttcc cggcctgcac ggggcggaca
      241 aaaaagggga gccgggcccg catctgtccc ggcctccggc tcccgcccgt gcgctgccgc
      301 tttctggtcc gccctcaggc gaccatccgc ttgacgaggt ccagcattgc gcccacgttg
      361 ggcaggtttt tgacgtcgtc cagcttgaat ttgatggaga actttttctc gatcgccacc
      421 agcaggttga tctgctccag gctgtcccaa tcctcgatgt cgtctgcggt catttcccgg
      481 gtcaacacga tgccgtcgtc gtcaaatacg gtgcggaaaa tctcctgcac gctgtcgaaa
      541 atttcctgtt cgctcaaaat agtttcctcc tgattattct tttgtggtca ccgcaaggat
      601 gtcgttctgc ttttgataag cggcgacgtc gagcgaaaag acggtttccg catcgttttc
      661 actcactttt tcaaagccca tggcgccgta gatctctttc accagcaaat ttttggcggt
      721 gggcagatac cggcccacga tttttttgat gccgcgcgcg gcacagcggc gcacgaactc
      781 gtccatcatc gcctgttcca ggttgcgctt gaaaacccgg cagctcatga tccacaaatc
      841 gacggtgcag acgtcgccct ccacactgcc gatcatggcc gagacaaggc cgttgtcgcc
      901 gaacttgtcg gtcagacgcc catagagggt gaccgcggca gaactgccga tcagggcctc
      961 cacctcctgc gccgtatagc gccgggtggt cagattgaac tggttggtct tgttgatgag
     1021 ctgggtgatg cgctcggcgt ggggctggtc aaatccgccc agttccgcgg tcatctccag
     1081 actgcgcagg taatcgtcgt agctgccgaa gctctgctgc accgccgccc gcgccagatt
     1141 ctggcgatac atctcgttgc gcttggcgtc gtccgccgag agggtggtga cctcaaaata
     1201 gcccgcccgg tccagcgagg cgatcatgcc ctccggcc
//
LOCUS       DBMDYA010000774         1234 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20507_length_1234_cov_1.889673, whole genome shotgun sequence.
ACCESSION   DBMDYA010000774 DBMDYA010000000
VERSION     DBMDYA010000774.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20507_length_1234_cov_1.889673"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="ACZPFK_14085"
     CDS             complement(<1..380)
                     /locus_tag="ACZPFK_14085"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1407485.1"
                     /translation="MSKIPVILDVDTGVDDAVAIMYALTCEELDILGISTVAGNQTLD
                     KTTRNTLAVLEYLGRPEVPVARGAAGPFCRPLHTAPQVHGENGLGNVELPEPVKQPEA
                     DDALTCLRRWIEGSAQPVTIIAVGP"
     gene            557..1219
                     /gene="yeiL"
                     /locus_tag="ACZPFK_14090"
     CDS             557..1219
                     /gene="yeiL"
                     /locus_tag="ACZPFK_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HIR1407486.1"
                     /translation="MAEEWRLEPTLEKYRFREYFSFDITPYASLAVFEPGELILREGQ
                     RVTRLYYLVRGRAKLFTTLKNGKVTLFNFYEVPSFLGEMEMLDPEAHTKGVRAHTRCY
                     CIALDLTRCGERVLNDPRFLRAICLYLGRKAMGNSWHYAQSQSYPLKYRLASYILFTS
                     RDGVYAEPHTEAAEYLGISYRHLLYVLAGFCREGILERTPRGYLLRDPGRLETLRAEM
                     QE"
BASE COUNT          235 a          354 c          406 g          239 t
ORIGIN      
        1 ggacccacgg cgatgatggt gaccggctgt gcgctgccct cgatccagcg gcgcaggcag
       61 gtgagggcgt cgtccgcctc cggctgcttg acgggctcgg gcagctccac gttgcccagg
      121 ccgttttcgc cgtgcacctg gggcgcggtg tgcagcgggc ggcagaaagg cccggcggcc
      181 cccctggcca ccggcacctc gggacggccc agatactcca gcacggcaag ggtgttgcgg
      241 gtggtcttgt ccagcgtctg gttgccggcg accgtggaaa tgcccaggat gtccagctct
      301 tcgcaggtca gtgcgtacat gatcgccaca gcgtcatcca cgcccgtatc gacgtcgaga
      361 ataacaggga tcttgctcat gaaatggctc ctttcaatca gatgggtctt tggtgacaga
      421 taggtctttg gtgatcacct ttcgattgca tcttagcatc agatgggata aaatgatatg
      481 agaaatctca catagaacaa aatagtcgtg aaaaattcgg ccgcgcgcgg cgggcctgca
      541 aaaggagcga gagaccatgg cggaagaatg gaggctggag cccacactag aaaaatatcg
      601 ctttcgggag tacttttcct ttgacatcac cccctatgcc agcttggcgg tatttgagcc
      661 gggggagctg atcctgcgcg agggccagcg ggtgacccgg ctctattatc tggtaagggg
      721 gcgggccaag ctgttcacca ccctgaaaaa cggcaaggtg accctgttca atttttatga
      781 ggtgcccagc tttttgggcg agatggagat gctggacccc gaggcgcaca ccaaaggggt
      841 gcgcgcacac acccgctgct attgtatcgc gctggacctc acccgctgcg gggaacgggt
      901 gctgaacgac ccccgctttc tgcgggcgat ctgtctctat ctgggccgca aggcgatggg
      961 gaactcctgg cactacgccc agagccagtc ctatccgctg aaataccggc tggcctcgta
     1021 tatccttttt accagcaggg acggcgtcta cgccgagccc cacaccgagg cggcggagta
     1081 tctgggcatc agctaccggc atctgctgta tgtgctggcc ggcttttgcc gggagggcat
     1141 tctggagcgc acgccgcggg ggtacctgct gcgggacccg gggcgtctgg agacgctgcg
     1201 ggccgagatg caggagtaga gggaggcagg gccg
//
LOCUS       DBMDYA010000775         1234 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20509_length_1234_cov_1.407767, whole genome shotgun sequence.
ACCESSION   DBMDYA010000775 DBMDYA010000000
VERSION     DBMDYA010000775.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20509_length_1234_cov_1.407767"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="ACZPFK_14095"
     CDS             <1..616
                     /locus_tag="ACZPFK_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1407487.1"
                     /translation="NGEGACPVCGGKGVIVSDMSFMDSIETACEACHGLRYSPQALAH
                     TVDGLNIAQVMDLSVEQAARWFAGTGIADKLRPLERVGLGYLHLNQSLSTLSGGELQR
                     MKLASYLGSKGEIFILDEPTDGLHLSDIHRIIQLFDDMVEQGNSVFLVEHSLEVLAAA
                     DYVVELGPGGGGAGGELLFAGTPRQLLACPASVTAPYLRAELEQ"
     gene            complement(848..>1234)
                     /locus_tag="ACZPFK_14100"
     CDS             complement(848..>1234)
                     /locus_tag="ACZPFK_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407488.1"
                     /translation="LIATLVLLIGGGAYFTLFVYQGSHVSPAQVAASAAMTPEGRVAL
                     RLQPQDGHCGGALTAQTDAEGNLYVSVARTLVKEKLPDGEEEVMIYGFDPAEKGYKAV
                     YYGTPKDCTLLWKKGDTLPAADGSFR"
BASE COUNT          207 a          388 c          421 g          218 t
ORIGIN      
        1 caacggggag ggcgcctgcc cggtctgcgg gggcaagggc gtgatcgtgt cggacatgtc
       61 ctttatggac tccatcgaga cggcctgtga ggcctgccac gggctgcgct attcgcccca
      121 ggcgctggcc cacacggtgg acgggctgaa tatcgcccag gtgatggacc tgtcggtgga
      181 gcaggccgcg cgctggttcg ccggcaccgg catcgctgac aagctgcgcc ctctggagcg
      241 ggtgggcctg ggctatctgc acctgaacca gtccctttcc accctttcgg gcggcgagct
      301 gcagcgcatg aagctggcct cctatctcgg cagcaagggg gagatcttca tcctggacga
      361 gcccaccgac gggctgcatc tcagcgatat ccaccgcatc atccagctgt tcgacgacat
      421 ggtggagcag ggcaactcgg tctttctggt ggagcacagc ctggaggtgc tggccgcggc
      481 cgactatgtg gtcgagctcg ggcccggcgg gggcggggcg ggcggcgaac tgctgtttgc
      541 aggcacgccg cggcagctgc tggcctgccc ggcatcggtg accgcaccct acctgcgggc
      601 agagctggag caataaaaaa cagccgcagc aaagcggagg ctgttttttc ggccatccgt
      661 ttcggttgcc gcgcagcggc gggaaacggg gtcatcaaaa taaggcgttt ttacgcgcgt
      721 ccgccccctc tgcggacggc gctcgagacc tcaagagtaa gggccgtgcc gcagaatgcg
      781 gcacggccct tgtggtcccg gcggcagccc tgcgcggggc gccgacgcgg ccgcatcggt
      841 tttgcggcta gcgaaaactg ccgtcagccg cgggcagcgt atcccctttt ttccagagca
      901 gggtgcagtc tttgggagtg ccgtaataga cggccttata gcctttttca gccgggtcaa
      961 aaccatagat catgacctct tcctccccgt ccggcagctt ttccttgacc agggtgcgcg
     1021 cgacggagac atacaggttg ccctctgcat ccgtctgggc agtaagcgcg cctccgcagt
     1081 gaccgtcctg cggctgcagg cgcagggcga cgcgcccttc cggcgtcatg gcggcggagg
     1141 cggcgacctg cgccggggaa acgtggcttc cctgatacac aaacagggtg aaataggctc
     1201 caccgccgat caagaggacg agggtggcaa tcaa
//
LOCUS       DBMDYA010000776         1231 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20568_length_1231_cov_1.645133, whole genome shotgun sequence.
ACCESSION   DBMDYA010000776 DBMDYA010000000
VERSION     DBMDYA010000776.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20568_length_1231_cov_1.645133"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="ACZPFK_14105"
     CDS             <1..99
                     /locus_tag="ACZPFK_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198047.1"
                     /note="similar to novel fructose-6-phosphate aldolase from
                     Escherichia coli; enzyme from Methanocaldococcus janaschii
                     shows transaldolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1407489.1"
                     /translation="VPYKVIEQMTKHPLTDQGIEKFKADYIKVFGE"
     gene            214..978
                     /locus_tag="ACZPFK_14110"
     CDS             214..978
                     /locus_tag="ACZPFK_14110"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1407490.1"
                     /translation="MTNAEKKTLAATACKVRMGVIEGVHGAKAGHPGGSLSAADLFTY
                     LYFKEMKVDPKNPRWADRDRFVLSKGHTAPGLYAALALKGFFPVEDLPTLRHIDSYLQ
                     GHPNMNTVPGIDMSTGSLGQGVSTAVGMALAAKATGKEYRTYTLLGDGEIQEGQVWEA
                     FMSAAHYKLDNLLVFIDNNGLQIDGEVAKVMSPYPIDEKMKAFGFNVINIKTTKGKGV
                     SYMENQAGWHGKAPNDEQYEIAMNELKAQLAEVEAK"
     gene            981..>1231
                     /locus_tag="ACZPFK_14115"
     CDS             981..>1231
                     /locus_tag="ACZPFK_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1407491.1"
                     /translation="MAEVKKIATRESYGNALVELGKEHENLVVLDADLAEATKTGIFQ
                     KAFPERHFDFGIAEANMMDAAAGMSTAGLVPFVSTFAMFA"
BASE COUNT          277 a          378 c          380 g          196 t
ORIGIN      
        1 gtgccctaca aggtgatcga gcagatgacc aagcacccgc tgaccgacca aggcatcgag
       61 aagttcaagg ccgactacat caaggtgttc ggcgagtaat tttttgcaaa cgcgcgcttc
      121 ccctccgggc cgtggcccgg ggccagggga ggggccgagt tccagccgga gggcgttgac
      181 ccgccgcccg gcgtgaaagg atgataacag agcatgacta acgctgagaa aaagacgctc
      241 gccgccaccg cctgcaaggt gcgcatgggt gtcatcgagg gtgtgcacgg cgcaaaggcc
      301 ggccatccgg gcggcagcct gtccgccgcc gacctgttta cttatctgta tttcaaagag
      361 atgaaagtgg acccgaagaa cccgcgctgg gctgaccgcg accgctttgt gctgtccaag
      421 ggccacaccg cgccgggcct gtatgccgcc ctggcgctga aaggcttctt ccccgtggag
      481 gatctgccca ccctgcgcca catcgacagc tacctgcagg gccaccccaa catgaacacc
      541 gtgcccggca tcgacatgtc caccggctcg ctgggccagg gcgtttccac cgccgtgggc
      601 atggcgctgg ccgcaaaggc caccggcaag gagtaccgca cctacacgct gctgggtgac
      661 ggcgagatcc aggagggcca ggtctgggag gcgttcatgt ccgccgctca ctacaagctg
      721 gacaatctgc tggtctttat cgacaacaac ggcctgcaga tcgacggcga ggtggccaag
      781 gtcatgagcc cgtaccccat tgatgaaaag atgaaagctt tcggcttcaa cgtcatcaac
      841 atcaagacca ccaagggcaa gggcgtcagc tatatggaga accaggcggg ctggcacggc
      901 aaggccccca acgacgaaca gtatgagatc gccatgaacg agctcaaggc acagcttgca
      961 gaagtggagg cgaaatgaat atggcagaag taaaaaagat cgccacccgc gaaagctatg
     1021 gcaacgcgct cgtggagctg ggcaaagagc atgagaacct ggtcgtgctg gacgccgacc
     1081 tggccgaggc caccaagacc ggcatttttc agaaagcgtt ccccgagcgg cacttcgact
     1141 tcggcatcgc cgaggccaac atgatggacg ccgccgcagg catgagcacc gcagggctgg
     1201 tgccctttgt gtccaccttt gcgatgttcg c
//
LOCUS       DBMDYA010000777         1229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20612_length_1229_cov_0.987589, whole genome shotgun sequence.
ACCESSION   DBMDYA010000777 DBMDYA010000000
VERSION     DBMDYA010000777.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20612_length_1229_cov_0.987589"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="ACZPFK_14120"
     CDS             <1..778
                     /locus_tag="ACZPFK_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR1407492.1"
                     /translation="AVLNLDDPYGRRLRDEIPRQRVITYSTALDEADLVAKNLELHAG
                     GVRFEMVGEGFIRRVKFPMPGSYSAHNALAAAGAAAALGVDPAVAADALTRSPGVHGR
                     CEVLYSGRFTMICDFAHTGDGIEKVLAGLAPFVQGRLVVLFGCAGERDARKREPMALA
                     ALRYGDLIVLTSDNPRGEDPYHILADVEPTLKTGGKPYLVEVERRRAIRLALEQLRDG
                     DVLVLCGKGHEDYQAIDGVTIYLDEHRIVADWLREQGLAE"
     gene            817..>1229
                     /locus_tag="ACZPFK_14125"
     CDS             817..>1229
                     /locus_tag="ACZPFK_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR1407493.1"
                     /translation="MNAIPLKALLDGVAVTDDLSPVTAVVTDSRAAGPGSVFIAIKGE
                     RVDGHDYAARALAGGAAAVVAEREIPGLPRERVFAVGNVRDAMIRMGGNYRALYAPVL
                     VGVTGSVGKTTTKEFCAAVFSAFGETLKTEGNQNN"
BASE COUNT          197 a          392 c          453 g          187 t
ORIGIN      
        1 ggcggtgctc aacctcgacg acccctacgg gcggcggctg agagacgaga tcccccggca
       61 gcgggtgatc acctattcca ccgcgctgga cgaggcggac ctggtggcga aaaacctgga
      121 gctgcacgcg ggcggcgtcc gctttgagat ggtgggggag ggattcatcc gcagggtgaa
      181 gttccccatg ccgggcagct attccgccca caacgccctg gccgcggcgg gggccgcggc
      241 ggcgctgggg gtcgaccccg ctgtggccgc ggatgcgctc acccgttcgc cgggggtgca
      301 cgggcgctgc gaggtgctct acagcggccg cttcaccatg atctgcgact tcgcccacac
      361 cggcgacggc atcgaaaagg tgctggcggg ccttgcgccc tttgtccagg ggcggttggt
      421 ggtgctgttc ggctgcgcag gcgagcggga cgcgcgcaag cgggagccca tggctctggc
      481 ggccctgcgg tacggcgacc tgatcgtgct cacctccgac aacccgcgcg gggaggaccc
      541 ctaccatatc ctggcggatg tggagcccac cctgaagacc ggcggcaagc cctatctcgt
      601 cgaggtggag cggcggcgcg ccatccggct ggcgctggag cagctgcggg acggcgacgt
      661 gctggtgctc tgcggcaagg gtcacgagga ctatcaggcc atcgacgggg tgaccatcta
      721 cctcgacgag caccgcatcg tggccgactg gctgcgggag cagggcctgg cggaatgacg
      781 gtttgcgaca aaaacacagg acaggtgtga tatactatga acgcgatccc attgaaagcg
      841 ctgctggacg gcgtggcggt caccgatgac ctctcgcctg tgaccgccgt ggtcaccgat
      901 tcccgcgccg cggggcccgg cagcgtcttc atcgccatca agggcgagcg ggtggacggc
      961 cacgactatg cggcccgggc gctggctggc ggcgcggcgg cggtggtggc ggagcgtgag
     1021 atccccggcc tgccgcgcga aagggtgttc gccgtgggca atgtccggga cgcgatgatc
     1081 cgcatgggcg gcaattaccg cgccctctat gcgccggtgc tggtcggcgt caccggcagc
     1141 gtgggcaaga ccactacaaa agagttctgc gcagccgtct tttccgcttt cggcgagacg
     1201 ctgaagaccg agggcaacca gaacaacga
//
LOCUS       DBMDYA010000778         1225 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20680_length_1225_cov_1.451068, whole genome shotgun sequence.
ACCESSION   DBMDYA010000778 DBMDYA010000000
VERSION     DBMDYA010000778.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20680_length_1225_cov_1.451068"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="ACZPFK_14130"
     CDS             complement(<1..293)
                     /locus_tag="ACZPFK_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1407494.1"
                     /translation="MLHDPLVLAMLGTGFTFAATAVGAAAVFFVKKDASPTTHRAFLG
                     FAAGVMIAASVWSLLIPAQEMAAQQGMSEWLPTTLGFLTGGVFLFLLDHLLPHL"
     gene            complement(519..938)
                     /locus_tag="ACZPFK_14135"
     CDS             complement(519..938)
                     /locus_tag="ACZPFK_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407495.1"
                     /translation="MEERKTMFENAGRPGFNDDASTLDRFAHHALNNRPNDVHDTGRD
                     EAARRQAQRRQEEAEARAIERRVSEGPVSDRQCAGRTNCIPQSQRNAQAQSRQQIKGN
                     LYYTQMDVELKDHGRRYTESDETVVSKMISKEEFPLG"
BASE COUNT          216 a          398 c          357 g          254 t
ORIGIN      
        1 aggtggggca gcaggtggtc cagcagaaac agaaacaccc cgccggtcaa aaagcccagc
       61 gtggtgggca gccactccga catcccctgc tgcgccgcca tctcctgggc ggggatgagc
      121 aggctccaga ccgaggccgc gatcatcacc ccggccgcaa accccaaaaa agcccggtgg
      181 gtggtcgggg aggcgtcttt tttgacaaaa aagaccgcgg ccgcgcccac cgcggtggcg
      241 gcaaaggtaa aaccggtgcc gagcatggcc aggaccaaag gatcgtgcag catagagcca
      301 tctcctcttt attgataatt gttatcaata aaagtataac acggctttta taaaaacacc
      361 agcccttttt gtcattttgt gcgcaaggcg atgaaaaaaa ggatagccgg ggcgggctct
      421 cgcaaagagc ggggctgccg tcctgccgac aaagggcaaa agcgcgcttt gcgggccccg
      481 ctttggggcc tggcagagcg cgcttttgcc ccggccggtc agcccaaggg gaattcctct
      541 ttgctgatca tcttgctgac caccgtctcg tcgctctcgg tatagcggcg gccgtggtct
      601 ttcagctcca catccatctg ggtgtagtaa agattgccct tgatctgctg tctgctctgc
      661 gcctgggcgt tgcgttgaga ctgaggaata cagtttgtgc gccccgcgca ctggcgatcg
      721 ctcacgggcc cctctgaaac cctgcgctct atcgcgcggg cctccgcttc ctcctggcgc
      781 cgctgcgcct gccgacgcgc cgcttcgtcc cgacccgtgt cgtgcacgtc attgggacgg
      841 ttgttcagcg cgtggtgcgc aaagcggtcc aaggtgctgg catcatcgtt gaagccgggc
      901 ctgccggcat tttcaaacat tgtctttctc tcttccatgg gaaatccacc tccgccacct
      961 agtttgcccg cagcccggcc gcctatgcgc ccgccgcatc cgcccgcaca aatgcaaaac
     1021 gccccggccg gggccggagc ggcaatcttt ttcttctttt tgttcgcggt tgttttttta
     1081 cggcgcccga aatggccgcg gccttatgcc gccggctccg tccgccgttt tgcgctgaaa
     1141 gcggcgcttt tccccaaagg cgcggacgcc cgcccttttg acgcggcgaa tccgcgggtc
     1201 aagcttcctc tccctgcagg gcgct
//
LOCUS       DBMDYA010000779         1221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20769_length_1221_cov_1.407143, whole genome shotgun sequence.
ACCESSION   DBMDYA010000779 DBMDYA010000000
VERSION     DBMDYA010000779.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20769_length_1221_cov_1.407143"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="ACZPFK_14140"
     CDS             complement(<1..181)
                     /locus_tag="ACZPFK_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407496.1"
                     /translation="MKLHYAGKYDLNPASLPSGPHRPGAVPFREAQDSGELSRLCTRL
                     SLLLLLALGGLLVLRC"
     gene            complement(199..954)
                     /locus_tag="ACZPFK_14145"
     CDS             complement(199..954)
                     /locus_tag="ACZPFK_14145"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1407497.1"
                     /translation="MKLISWNVNGLRACLTKGFTGFFSQLDADVFCVQETKMQQGQAE
                     LELPGYRQYWNSAERKGYSGTAVFARREPLAVTYGLGIEEHDHEGRVITLEYPQFYLV
                     NCYTPNSKRGLERLEYRMQWEDAMRAYLCALDERKPVVYCGDLNVAHQPIDLKNAKSN
                     EGNSGYTLEERGKMTELLESGFLDSFRALYPEKTGAYSWWSYMFNARANNAGWRIDYF
                     IISERLRGAVVDSTIWPEVTGSDHCPVGLELDF"
BASE COUNT          210 a          425 c          364 g          222 t
ORIGIN      
        1 cgcagcgcag aacgagcagg cccccaaggg ccagcagcag caaaagggag aggcgggtgc
       61 acagccggct cagctcgccg ctgtcctgcg cctcccggaa cggcaccgcc ccgggccggt
      121 gcggcccact gggcaaggag gccgggttca ggtcatattt gcccgcgtag tgcagcttca
      181 ccgccgcccc ctcctctctc aaaaatccag ctccagcccc accgggcagt gatcgctgcc
      241 ggtcacctcg ggccagatgg tgctgtccac caccgcgccg cgcagccgtt cgctgatgat
      301 gaagtagtcg atgcgccaac ccgcgttgtt ggcgcgggcg ttgaacatat agctccacca
      361 gctgtaggcg cccgtctttt cggggtaaag ggcgcggaag ctgtccagaa agccgctctc
      421 cagcagctcg gtcatcttgc cccgctcctc cagcgtgtag ccggagttgc cctcgttgct
      481 cttggcgttt ttcaggtcga tgggctgatg ggccacgttc aggtcgccgc agtagaccac
      541 cggctttctc tcatccaggg cgcagagata ggcgcgcatc gcgtcctccc actgcatgcg
      601 gtactccagc cgctccagcc cccgcttgga gttgggggtg tagcagttga ccagataaaa
      661 ctgcggatat tccagcgtga tgacccgccc ctcgtggtcg tgctcctcaa tacccaggcc
      721 ataggtgacg gccagcggct cccgccgcgc aaagaccgcc gtgccggaat agcccttgcg
      781 ctcggcgctg ttccaatact gccggtagcc cggcagctcc agctcggcct gcccctgctg
      841 catcttggtc tcctgcacac agaagacgtc ggcgtcgagc tggctgaaaa agcccgtgaa
      901 ccccttggtc aggcaggccc gcaggccgtt gacgttccag gagatcagct tcatgagatc
      961 catcctcccc ggcggcgctc cgccgctccg caaactgttt tgcggatatt gtaccatatc
     1021 cgacaaaaaa agacgaggtg gcccgcccgc cccggctcat caaaaaggga tacggcgcct
     1081 tgacgccgta tccctttttc ttttttcgtc tttggcccgg cctcaggcct cggcccgctt
     1141 gtcgcggctc atcagcaggg tggcgatgaa cgccagcacc gtgcagacca gccccagcgc
     1201 cgcccaggcg gtgagggaga a
//
LOCUS       DBMDYA010000780         1219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20815_length_1219_cov_1.554562, whole genome shotgun sequence.
ACCESSION   DBMDYA010000780 DBMDYA010000000
VERSION     DBMDYA010000780.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20815_length_1219_cov_1.554562"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="ACZPFK_14150"
     CDS             <1..660
                     /locus_tag="ACZPFK_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1407498.1"
                     /translation="PYGCGPGPYESQQTEVRVTSRSALEAVTGGEGFTAQDRGFAAYA
                     DPADADALTRRFEALFSRQGNLRWVASDYTAGSAWANQRAAVPLLLNTFLGGFLALVL
                     AFCAAGVLSTVGAGLQLRRRELALLRAAGASTAQLRRMLALEAVSYGAAGVLFGLPLG
                     AAFLALIGRMLWTGLFDALSPLPVLSATLGAALVSGLALAVSLRSLDRLPLAEALSCG
                     E"
     gene            815..>1219
                     /locus_tag="ACZPFK_14155"
     CDS             815..>1219
                     /locus_tag="ACZPFK_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1407499.1"
                     /translation="MNAKRVKDLLLDLVYDVVGSVVYGVGIYLFASQGGFATGGFSGL
                     GLMVNHLTGLPIGTLTLVFNIPVVILSYKTLGKVFLAKSLKTMVIQTLILDLVMPLFP
                     VYRGNQLLAALFTGVLMGAGLVLVYMRGSSTGG"
BASE COUNT          149 a          406 c          425 g          239 t
ORIGIN      
        1 ccctatggct gcggccccgg cccctacgag agccagcaga ccgaggtgcg ggtcaccagc
       61 cgcagcgccc tggaggcggt caccggcggg gagggcttca ccgcgcagga ccgcggcttt
      121 gccgcttacg ccgacccggc ggacgcggac gccctcaccc ggcgttttga ggcgctgttc
      181 agccgccagg gaaacctccg ctgggtcgcc tcggattaca ctgccggttc cgcgtgggca
      241 aaccagcgcg ccgcggtgcc cctgctgctg aacacctttc tcggcggttt tctggctctg
      301 gtgctggctt tttgcgccgc cggcgtgctc agtacggtgg gcgcggggct gcagctgcgc
      361 cgcagagagc tggccctgct tcgcgcggcg ggcgcgtcca cggcccagct gcggcggatg
      421 ctggctctgg aggcggtctc ctacggggcg gcgggcgtcc ttttcggcct gcccctgggc
      481 gcggcctttc tcgccctgat cggccggatg ctctggacgg gattgttcga cgccctttcc
      541 cccctgccgg tgctctcggc aacgctgggg gcggcgctgg tcagcgggct ggccctggcg
      601 gtcagcctgc gctcgctgga tcggctgccc ctggccgaag ccctctcctg cggcgagtga
      661 gggcgcttcc cctccccggc tctgcgctcc actccccgcc tcctgctccc ccgccggggc
      721 cccggcgggg gattttctgt gcccgggggt atcgcggcca aaatggatat gatacaatgg
      781 aggagaaaaa ggcttgacgg caagggggag ttgtatgaac gcgaaacgcg tcaaggatct
      841 gctgctggac ctggtgtacg atgtggtggg cagcgtggtg tatggggtgg gcatctacct
      901 ttttgccagc cagggcggtt ttgccaccgg cggcttttcg ggcctggggc tgatggtgaa
      961 ccatctcacc ggcctgccca tcggcacttt gaccctggtg ttcaacatcc cggtggtgat
     1021 cctcagctac aagacgctgg gcaaggtgtt tttggccaaa agcctcaaga ccatggtcat
     1081 ccagaccctt atcctggatc tggtgatgcc gctgttcccg gtctaccggg gcaaccagct
     1141 gctcgccgcc ctcttcacag gggtgctgat gggcgcgggg ctggtgctgg tgtatatgcg
     1201 cggctcctcc accggcggc
//
LOCUS       DBMDYA010000781         1218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20836_length_1218_cov_1.788720, whole genome shotgun sequence.
ACCESSION   DBMDYA010000781 DBMDYA010000000
VERSION     DBMDYA010000781.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20836_length_1218_cov_1.788720"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="ACZPFK_14160"
     CDS             complement(<1..458)
                     /locus_tag="ACZPFK_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1407500.1"
                     /translation="MKMILDLDTGIDDALAIAYALGLPGVELIGVTNCFGNVTIDTAV
                     QNTLDLLELLGHPEVPVFEGASCPWGADGYTLAPHKFKIHGQNGIGNVELPRAKAQKS
                     AVSAVDFILDSARRYGPDLALVATAPLTNLANAIAKDKNAVGRIGRISIMG"
     gene            571..>1218
                     /locus_tag="ACZPFK_14165"
     CDS             571..>1218
                     /locus_tag="ACZPFK_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1407501.1"
                     /translation="MTEQARVELLQKKIEEYRLEELIPPRHYPQVRYLELPKGTHLFR
                     QNEPAEYFYIMLRGSGRLYQILGNGKSTTNEVVTGFCLLGDMEMLCDKPTLNSFQLLE
                     AGEFLRLPMDYCRREMIGEHGFLKALAAHLARALYTVEANNYVALAFPFEGQLANYIL
                     SLQKETFALDLRLLPEQFGATYRHFLRVVKKLCEEGAIEKQGKLYRIKDRSALERK"
BASE COUNT          252 a          348 c          376 g          242 t
ORIGIN      
        1 cccatgatgg agatgcggcc gatccgcccg acggcgtttt tgtcctttgc gatggcgttt
       61 gccagattgg tgagcggggc ggtggcgacc agcgccagat cggggccgta gcggcgcgcg
      121 gaatcgagga tgaaatccac cgcggagaca gcgctctttt gcgctttggc ccggggcagc
      181 tccacgttgc cgatgccgtt ttggccgtgg atcttgaatt tgtggggcgc cagcgtgtag
      241 ccgtcggccc cccaggggca ggacgcgccc tcaaacaccg gcacttccgg gtgccccagc
      301 agctccagca ggtccagcgt gttctgcact gcggtgtcga tggtgacgtt gccaaaacag
      361 ttcgtgaccc cgatcagctc caccccgggc aggcccaggg cgtaagcgat ggccagcgcg
      421 tcgtcgatgc cggtgtccag gtccagtatc atcttcatct gaaagtcctc ctattgttgg
      481 ctttttttcc gcttcacagt ataataaaaa gaagaaaagg gtttcgatga catttgtcac
      541 aaaaaagaaa aattaggagg agggatcggc gtgacggagc aagcgcgggt ggagcttttg
      601 caaaaaaaga tcgaagaata ccgcctcgaa gagctgatcc cgccccgcca ctatccccag
      661 gtgcgctatc tggagctgcc caaggggacg cacctgttcc ggcagaacga gcccgcggag
      721 tatttttaca tcatgctgcg gggcagcggg cggctctacc agatcctcgg caacggcaaa
      781 tccaccacca acgaggtggt gaccggcttt tgccttttgg gggacatgga gatgctctgc
      841 gacaagccga ccctgaactc gttccagctg ttggaggcgg gggagttctt gcgcctgccc
      901 atggactact gccgccgcga gatgatcggg gagcacggct ttttaaaggc cctggcggcc
      961 cacctggcaa gggcgctgta caccgttgag gcgaacaatt atgtcgccct cgcctttccc
     1021 tttgagggac agctggcaaa ttatatcctc tctctgcaga aagagacctt tgcgctggat
     1081 ctgcgcctcc tgcccgagca gttcggcgcc acctaccggc actttctgcg ggtcgtgaaa
     1141 aagctttgcg aggagggggc aatcgaaaag cagggcaagc tctaccgcat caaagacaga
     1201 agcgcgctgg aaagaaaa
//
LOCUS       DBMDYA010000782         1215 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20901_length_1215_cov_1.295332, whole genome shotgun sequence.
ACCESSION   DBMDYA010000782 DBMDYA010000000
VERSION     DBMDYA010000782.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20901_length_1215_cov_1.295332"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="ACZPFK_14170"
     CDS             <1..398
                     /locus_tag="ACZPFK_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR1407502.1"
                     /translation="ITAGVPVGVSGSTNMIKAHLVGASLLSGVGVGNLVGVGRICICK
                     SEQEIKSKFRPGDVLVLPSTSNDILDAMKKASAIICEEPGLNSHAAIVGLTLEKPVIV
                     GATGATHKLKDGVKVAVDAERGVVRVMPQ"
     gene            416..880
                     /locus_tag="ACZPFK_14175"
     CDS             416..880
                     /locus_tag="ACZPFK_14175"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1407503.1"
                     /translation="MELQRIASFSVDHNRLQKGMYTSRVDGDVVTYDIRMKRPNQGDY
                     LSCGALHTFEHLFATYARSSCYQDGVLYIGPMGCRTGFYLLVRGLSQQQAIDLVRESF
                     AFIAGFEGDIPGAAQAECGNYLEHDLPGAKEVARDMCRVLEFWTPDDLAYER"
BASE COUNT          233 a          386 c          362 g          234 t
ORIGIN      
        1 tcatcaccgc cggggtgccg gtgggggtct cgggctccac caacatgatc aaggcccacc
       61 ttgtgggcgc ctcgctgctg tccggcgtgg gggtgggcaa tctggtgggc gtcggccgca
      121 tctgcatctg caagagcgaa caggagatca agtcgaagtt ccgccccggc gatgtgctgg
      181 tgctgccctc caccagcaat gatatcctcg atgcgatgaa aaaagcctcc gccatcatct
      241 gcgaggagcc cggcctcaac agccatgcgg ccatcgtcgg cctgacgctg gaaaagccgg
      301 tcatcgtggg cgccaccggc gccacccaca agctgaaaga cggcgtcaag gtggccgtgg
      361 acgccgagcg cggcgtcgtg cgcgtcatgc cgcagtaaaa aagggaggtc ccgttatgga
      421 attacagcgc atcgccagct tttcggtcga ccacaaccgg ctgcagaaag gcatgtatac
      481 ctcccgcgtc gacggcgacg tggtgaccta tgacatccgc atgaagcgcc ccaatcaggg
      541 cgactatctc tcctgcggcg ccctgcacac ctttgagcac ctgttcgcca cctatgcccg
      601 cagctcttgc tatcaggacg gggtgctcta catcggcccc atgggctgcc gcaccggctt
      661 ttacctgctg gtgcgcggcc tgtcgcagca gcaggccatc gacctggtgc gggagagctt
      721 tgctttcatc gcgggctttg agggcgatat cccgggcgcg gcgcaggccg agtgcggcaa
      781 ctatcttgaa cacgacctgc ccggggcaaa agaggtggcg cgcgacatgt gccgggtgct
      841 ggagttctgg acgcccgacg acctggccta cgagcgatag cggcaagccg ctttccccct
      901 ctccggttcc ccccgtgccc ggccgccggg cgggaaaggc cgcgcgtttt tattcctccg
      961 caataaaaac gacttatcat gatggcccat ttcccgccgc tgccgcggca accgaaatga
     1021 atggccacaa aaatagcctg cggctatttt ttattgctcc gcaataaaaa acaacttatt
     1081 ttaatggccc tgtttctcgc cgctgcgcgg taatcgaaac agatggccga aaaaatagcc
     1141 tccgcttcgc tgcggctatt ttttattgct ccgcaataaa aaactggtct ctacggccgt
     1201 ggcccgggga tgggc
//
LOCUS       DBMDYA010000783         1213 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_20948_length_1213_cov_1.080036, whole genome shotgun sequence.
ACCESSION   DBMDYA010000783 DBMDYA010000000
VERSION     DBMDYA010000783.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20948_length_1213_cov_1.080036"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="ACZPFK_14180"
     CDS             <1..235
                     /locus_tag="ACZPFK_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1407504.1"
                     /translation="AVVALNAKDKFGAMLVVGIIAQVTVQAVLNIAVVTNTIPNTGIS
                     LPFFSYGGTSLMMLLGEMGIVLSVSRQGRSPKT"
     gene            349..>1213
                     /locus_tag="ACZPFK_14185"
     CDS             349..>1213
                     /locus_tag="ACZPFK_14185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR1407505.1"
                     /translation="MRVLIAAGGTAGHINPALAIAAYLKERRPDTEIAFAGRREGMEY
                     GLVQKQGYPFYHIEVQGIQRSLTPHNIVRNLKALWYLSSAGRKSRRILREFAPDLVIG
                     TGGYVSGPVVLSAAKQGIPTAIHEQNAFPGVTNKILAKQVDAVFAAMPDAVEKLGVPH
                     KTIVTGNPVRPEILRADREGARAKLGVRPGQVCLLSFGGSLGARTINQVVADLAAWHI
                     RERDFFHIHATGGYGVELFHDLCQEKGILGDSHLLVKEYIDDMASCLAAADLVISRSG
                     AITISELAAAGR"
BASE COUNT          207 a          399 c          417 g          190 t
ORIGIN      
        1 cgcggtcgtc gcgctcaacg ccaaggataa gttcggcgcc atgctggtgg tgggcatcat
       61 cgcccaggtg acggtgcagg cggtgctcaa catcgccgtg gtcaccaaca ccatccccaa
      121 caccggcatc agcctgccgt ttttcagcta cggcggcacc tcgctgatga tgctgctggg
      181 cgagatgggc atcgtgctgt cggtcagccg gcaggggcgg tcgcccaaga catagcgttt
      241 ccgtcgcttc gcgcgggcgc gtttccccga ggggcgcgcg cccgcgccgg ggcggcggtt
      301 ttcggcaatt ccgggccgcg aggcccctga ggaaagggag agtgtgagat gcgcgttctc
      361 atcgcagcgg ggggcaccgc cgggcatatc aacccggcgc tggccatcgc cgcctatctg
      421 aaagagcggc ggccggacac cgagatcgcc tttgcgggcc gcagggaggg catggaatac
      481 ggcctggtgc aaaaacaggg gtatccgttt taccacatcg aggtccaggg catccagcgc
      541 agcctgaccc cgcacaacat cgtgcgcaac ctgaaagccc tttggtacct gtcctctgcg
      601 gggcgcaaga gcaggcgcat cctgcgcgag ttcgcgccgg acctggtcat cggcaccggc
      661 ggctatgtca gcggaccggt ggtacttagc gccgcgaagc agggcatccc caccgccatc
      721 cacgagcaga acgcctttcc cggcgtgacc aacaagatct tggcaaagca ggtggacgcg
      781 gtgttcgccg ccatgccgga cgcggtggaa aagctgggcg tgccccacaa gaccatcgtc
      841 accggcaacc cggtgcggcc ggagatcctg cgcgctgacc gggaaggggc ccgcgcaaag
      901 cttggggtgc ggccgggaca ggtctgcctg ctctcgttcg gcggcagcct gggcgcgcgc
      961 accatcaacc aggtggtggc cgatctggcc gcctggcaca tccgcgagcg ggactttttt
     1021 cacatccacg ccaccggcgg ctatggggtg gagctgttcc acgacctctg ccaggaaaag
     1081 ggcatcctgg gcgacagcca cctgttggtc aaggagtaca tcgacgacat ggcctcctgc
     1141 ctcgcggcgg cggacctggt catcagccgc agcggcgcca tcaccatcag tgagctggcg
     1201 gcggcggggc gcg
//
LOCUS       DBMDYA010000784         1196 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21306_length_1196_cov_1.135160, whole genome shotgun sequence.
ACCESSION   DBMDYA010000784 DBMDYA010000000
VERSION     DBMDYA010000784.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21306_length_1196_cov_1.135160"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="ACZPFK_14190"
     CDS             <1..696
                     /locus_tag="ACZPFK_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1407506.1"
                     /translation="ATVIRFCRKLGFKGFQGFKMILAAEMMQNNSRIQRENAENSSAQ
                     ERLFHMQEAYRQAVDETVRMLDLEKVEEACAAILSCKKLLFFGVGASGLTALEAKFKL
                     MRIGFNVDAATDGHMMAMQAALLESGGVAVGISYSGGAIDTVDALRAAKENGATTICF
                     THFYESEIMKYADICLMVGARELPTDCTALASKAAVLFVLDFLYGELTDRIKEAADSK
                     QIRTIDVVRERFI"
     gene            705..>1196
                     /locus_tag="ACZPFK_14195"
     CDS             705..>1196
                     /locus_tag="ACZPFK_14195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1407507.1"
                     /translation="MKKYRELSYGEFAPEVLLQPPFEDPPVIDDDLRARLNREGEAGI
                     CAEFSRVAAYPRMMEVRVRRTALKAGGGEVPLRIYHPLGAARAAVLFCHGGSFCFNSL
                     EVYDYILRYWAAFHGLLCVSVGYRLAPEHPFPQGLEDCWAALEWAQKELSGPKGLPLC
                     VAGD"
BASE COUNT          210 a          338 c          429 g          219 t
ORIGIN      
        1 gcgaccgtca tccgcttttg ccgcaagctg gggttcaagg gctttcaggg ctttaagatg
       61 atcctcgccg cagagatgat gcagaacaac tcgcgcattc agcgcgagaa cgccgagaac
      121 agcagcgcgc aggagcgctt gttccacatg caggaggcct accgccaggc ggtggacgag
      181 acggtgcgca tgctggattt agagaaggtg gaggaggcct gtgcggcgat cctgagctgc
      241 aaaaagctgc tctttttcgg cgtgggggcc tccgggctga cggcgctgga ggcgaagttc
      301 aagctgatgc gcatcggttt caacgtggac gccgccacgg atgggcatat gatggccatg
      361 caggccgcgc tgctggagag cgggggcgtg gcggtgggga tctcctattc cggcggggcc
      421 atcgacacgg tggatgcgct gcgcgcggca aaggaaaacg gggccaccac catctgcttc
      481 acccattttt atgagtcgga gatcatgaaa tacgccgata tctgcctgat ggtgggcgcg
      541 cgggagctgc ccaccgactg cacggcgctg gcctccaagg ccgcggtgct gtttgtgctc
      601 gactttttgt acggcgagct gaccgacagg atcaaagagg cggcggacag caagcagatc
      661 cgcaccatcg acgtggtgcg cgagcgcttt atctgagggg aaccatgaaa aaatacaggg
      721 aattgagcta tggcgaattc gcgccggagg tgctgctgca gccgccgttt gaggacccgc
      781 cggtgatcga cgacgacctg cgcgcccgcc tcaaccgcga gggggaggcg ggcatctgcg
      841 ccgagttcag ccgcgtggcc gcctacccgc gcatgatgga ggtgcgggtg cggcgcacag
      901 cgctgaaagc cgggggcggc gaggtgccgc tgcgcatcta tcacccgttg ggggcggcgc
      961 gcgcggccgt cctgttctgt cacggcggca gcttttgctt caactcgctg gaggtatacg
     1021 actatatcct gcgctattgg gccgcgttcc acggcctttt atgcgtgtcg gtggggtatc
     1081 gtctggcgcc ggagcacccg ttcccccagg ggctggagga ttgctgggcg gcgctggaat
     1141 gggcccaaaa ggagctgtcc gggcccaaag gcttgcctct ttgcgtggcg ggtgac
//
LOCUS       DBMDYA010000785         1195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21317_length_1195_cov_1.451554, whole genome shotgun sequence.
ACCESSION   DBMDYA010000785 DBMDYA010000000
VERSION     DBMDYA010000785.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21317_length_1195_cov_1.451554"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            214..>1195
                     /locus_tag="ACZPFK_14200"
     CDS             214..>1195
                     /locus_tag="ACZPFK_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase N-terminal domain-containing protein"
                     /protein_id="HIR1407508.1"
                     /translation="MDVVPEMLALLRRWGADAIRDCDGTAMPEELVHSGAKIYSTYYT
                     TRKDNAWAKANPDEVQQMYVSTRFVTATGPILSIHLMEGLYPELLRVNTHDDIRRWWE
                     VMDRTTGAVVPVQTWHYDCAAGDVVIENAEPFHDYSVSFLAYIIWDPVHMYNAVINGW
                     KDVEHQITFDVRQPKTHAYTLRRLRRYLEEHPEVLVLRFTTFFHQFTLVFDELAREKY
                     VDWYGYSASVSPYILERFEKEAGYRFRPEYIIDQGYHNCPYRVPSREFRDFQDFQMRE
                     VAALVREMTDICHEYGREAMMFLGDHWIGTEPFGPYFKNTGLDAVVGSVGN"
BASE COUNT          241 a          390 c          364 g          200 t
ORIGIN      
        1 cgggcctggc gctgctgctg gccgcgctgt atctctacaa caagaccttt accgacccaa
       61 agcccaaaaa gaaaaagcgc tgagcgcgca gggccgcttt gcccggcccc gcgccgcaaa
      121 acacaacgag caaatgaggt gaagcgattg tccaaccgcg acaacgaaaa ccaaacagcc
      181 gccctcaccg ggcgggtcac catccccacc gatatggacg tggtgcccga gatgctggcg
      241 ctgctgcgcc gctggggcgc ggacgccatc cgcgactgcg acggcaccgc catgcccgag
      301 gagctggtcc actcgggcgc caagatctac tccacctatt acaccacccg caaggacaac
      361 gcctgggcga aagcgaaccc ggacgaggtg cagcagatgt acgtcagcac ccggttcgtc
      421 accgccaccg ggcccatcct ctccatccat ctgatggagg ggctctaccc cgagctgctg
      481 cgggtgaaca cccacgacga catccgccgc tggtgggagg tgatggaccg caccaccggc
      541 gcggtggtgc cggtgcagac ctggcactac gactgcgcgg ccggggacgt ggtcatcgag
      601 aacgccgagc cgttccacga ctactcggtg agctttctcg cctatatcat ttgggacccg
      661 gtgcacatgt acaacgcggt gatcaacggc tggaaagacg tggagcacca gatcacgttc
      721 gacgtgcgcc agcccaagac ccacgcctac accctgcggc ggctgcgccg ttatctggag
      781 gagcaccccg aggtcttggt gctgcggttc accacctttt tccaccagtt caccctggtc
      841 tttgacgagc tggcgcggga aaaatatgtg gattggtacg gctactccgc ctcggtgagc
      901 ccctatatcc tggagcggtt cgaaaaggag gcgggctacc gctttcgccc cgaatacatc
      961 atcgaccagg ggtaccacaa ctgcccttat cgggtgccca gcagggagtt tcgcgatttt
     1021 caggactttc agatgcgcga ggtcgccgct ttggtgcggg agatgaccga catctgccac
     1081 gagtacggcc gcgaggcgat gatgtttttg ggcgaccact ggatcggcac cgagcctttc
     1141 ggcccctact tcaaaaacac ggggctggac gcggtggtgg gcagcgtggg caacg
//
LOCUS       DBMDYA010000786         1194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21344_length_1194_cov_1.345837, whole genome shotgun sequence.
ACCESSION   DBMDYA010000786 DBMDYA010000000
VERSION     DBMDYA010000786.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21344_length_1194_cov_1.345837"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(760..>1194)
                     /locus_tag="ACZPFK_14205"
     CDS             complement(760..>1194)
                     /locus_tag="ACZPFK_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1407509.1"
                     /translation="EGKQDVWGSYFLDYECHASGCEALDRVSIIYGHSIGDDPDGKCF
                     SQLKRYADPAFSAEHPYLNWQLADGGTLWQVFAAGEVPVTLNYLDPNPDDTACAQLLD
                     ALREAGGDGFEAVTVDGEDKLLILSTCTSDDAVRFVVAAKLL"
BASE COUNT          209 a          373 c          376 g          236 t
ORIGIN      
        1 catccggcca gtccgctggc cggggccatc aagataaatt gttttttatt gcggagcaat
       61 aaaaaatagc cgcaggctat tttttacggc catatggccc gtcctcgggc cacggccata
      121 gaaataagtt ttttattgca aagcaataaa aaaggccgta tggctccgcc gtcgggcgat
      181 gaccaatcgg ttgtgcccat ccgcagggtc cgggcccgga tgctttgaac ggcttgacgc
      241 gggcggaaac taagggcagg ggccggagac ggctccgtct ggatagggcg ttttcagagg
      301 gcgcggcccg tgcttgggcg gcgtcggcgt caaggtgaaa aaaataaaaa gcagccgcac
      361 cgcggctgct ttttttgctg tctgttctgg tcgtcgcagc agtggggaac ggggccgtcc
      421 cctgtctctt aaaataagcc gttttgggag atgcggctgc tttcagacgg aaccatcagt
      481 ataatttctt tttgcccggt tttgcaggtc ggccacctcg ccgggttcca tggacgttcg
      541 cgcccctcgg gcccggcttt acctgccgcc gccgtctttg tcagagggag gcacgatctg
      601 tgcgcctcga ggccacctgt ttcgccggaa acagcacatt ctccccctac ccgcccggcc
      661 cgcggcacat cccagctccc ctgagagggg agccgcggcc cgtggggcgt cttgccagcc
      721 gggccgcgcg cttttggccc tgcggggctt gcggcgccct cagagcagct ttgccgcgac
      781 cacaaagcgc accgcgtcgt ccgaggtgca ggtggagagg atgagaagct tgtcttcgcc
      841 gtccacagtg accgcttcaa agccgtcgcc gcccgcctcc cgcagggcgt ccagcagctg
      901 cgcgcaggcc gtgtcgtccg ggttggggtc cagatagttg agggtcaccg gcacctcgcc
      961 cgcggcaaac acctgccaca gcgtgccgcc gtccgccagc tgccagttca ggtaagggtg
     1021 ctcggcgctg aacgcggggt cggcgtagcg cttgagctgg gagaagcact ttccatcagg
     1081 gtcgtcccca atgctgtgcc cgtagatgat gctgacccgg tccagcgcct cacagccgga
     1141 ggcgtggcac tcataatcga gaaaatagga gccccagaca tcctgcttgc cctc
//
LOCUS       DBMDYA010000787         1194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21348_length_1194_cov_1.192132, whole genome shotgun sequence.
ACCESSION   DBMDYA010000787 DBMDYA010000000
VERSION     DBMDYA010000787.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21348_length_1194_cov_1.192132"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="ACZPFK_14210"
     CDS             complement(<1..787)
                     /locus_tag="ACZPFK_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1407510.1"
                     /translation="MERMRAFLIKFFYYTLIVAIAYCALKFVVPFFMPFVLAFLIAFL
                     LKTPANRLSKRLHLRRGPVAVVLLILFYTVLVTLTALLGTRLVLACVDALKALPQFYS
                     STVEPAIFQIEEWLSALFARLDPQLIAALDTLSQSLTDALGSAVSAISSAAIAATTGA
                     AGHIPAFFVKFLLMIVASFFFVVDYYKVASFLANLLPPRGKAMLFRIKQNGVDTLLKF
                     ARAYAMLLGLTFVELSVGLSLLGIKNAILIALGTAVVDILPVLG"
BASE COUNT          292 a          336 c          372 g          194 t
ORIGIN      
        1 tgcccagcac cggcaggata tccaccaccg cagtgcccag cgcgatgagg atggcgtttt
       61 taatgcccag cagcgaaagg ccgaccgaga gctcgacaaa ggtgagcccc agcagcatgg
      121 cgtaggcacg ggcgaacttc agcagcgtgt ccaccccgtt ctgcttgatg cggaacagca
      181 tcgccttgcc cctggggggc agcagattcg ccagaaaact ggccaccttg taatagtcca
      241 ccacaaagaa aaagctggcc acgatcataa gcaaaaactt gacgaaaaag gccgggatat
      301 ggccggcggc cccggtggtg gcggcgatgg cggcgctgga gatggcgctg acggcgctgc
      361 ccagcgcgtc ggtgaggctc tggctcaggg tgtccagcgc ggcgatgagc tgcgggtcca
      421 ggcgggcgaa cagggcgctc agccactcct cgatctgaaa gatggcgggc tcgacggtgg
      481 aggaatagaa ctggggcaga gctttcagcg cgtcgacgca ggccagcacc agccgagtgc
      541 ccagcagcgc cgtcagggtg accagcaccg tgtagaacag gatcagcagc accaccgcca
      601 ccgggccccg gcgcagatgc agccgctttg agagccggtt ggccggggtc ttgagcagaa
      661 aggcaatgag aaaggccagc acaaagggca taaaaaaggg caccacgaac ttgagggcac
      721 aataggcgat ggcaacgatc agggtgtaat agaaaaattt gatgagaaag gcgcgcatgc
      781 gctccacttc caacaggggc acctcccaaa agggcgttgt ttttgtcgcc ggggttttaa
      841 aaatttgaaa ttgaacgctt ctttttattt tacatacttt gtgccggttt taaaaggtga
      901 aaccgggctg tacacaataa ttttactttt gacccgcggc gcactccccc caaaaaagag
      961 aaaaacagga cgcaccgaca gtttggcttt tcagacggcc tgcggccgca ggcggaaacg
     1021 ccgctttttg ccgtgaaacg taaagcccga ggtgcgcgtt ctcgcctccc acccccggca
     1081 agaaagaagc tacgcgacaa atctgtgccg gagagaggac gggcgaggca cgatgtttta
     1141 aagagagaat tgccgcgcgt cgctgcggcc cccacagaca gcccgcagtc gccg
//
LOCUS       DBMDYA010000788         1193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21371_length_1193_cov_1.848901, whole genome shotgun sequence.
ACCESSION   DBMDYA010000788 DBMDYA010000000
VERSION     DBMDYA010000788.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21371_length_1193_cov_1.848901"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="ACZPFK_14215"
     CDS             <1..352
                     /locus_tag="ACZPFK_14215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1407511.1"
                     /translation="LLDEEGKLWISYFKLPSGQFIEVFPKAEGDRYTGDNRQCDRSHF
                     HGCFEVDARHEAYRDMEKKGVPITHTDDDSVGMCGSWCSFVSDPEGNQWEFMEFSPVS
                     QQIKDLPEDLNFKG"
     gene            367..903
                     /locus_tag="ACZPFK_14220"
     CDS             367..903
                     /locus_tag="ACZPFK_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1407512.1"
                     /translation="MDYLKRLTHCTFTCKDYPAMVRFYGETLGLKKVFTLPYNEDILQ
                     GFRDKGYQNVTAKPGDEWISYFEVAPKEFVELFNLPYNGENDTQDEGFHHFCLLVDDI
                     VAAARELEGKGVTLWNGPKWMNNPLTEPYPADPIPAGRQGQCGSLAFYIQDPEGNEIE
                     IMQYTPDSLQLKCQKVNG"
     gene            900..>1193
                     /locus_tag="ACZPFK_14225"
     CDS             900..>1193
                     /locus_tag="ACZPFK_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407513.1"
                     /translation="MKKGYQLFSAWKKCADAAGLKDVVRRIAAMGYDGVEFFSYAGVP
                     AAEMKALLDECGICAFNSHVQLERWQKDADFEIAYARALGMEALTISYIAPEDR"
BASE COUNT          255 a          352 c          385 g          201 t
ORIGIN      
        1 cctgctggac gaggagggca agctctggat ctcctacttc aagctgccca gcggccagtt
       61 catcgaggtc ttccccaagg cggagggcga caggtacacc ggcgacaacc gccagtgcga
      121 ccgctcgcac tttcacggct gctttgaggt ggacgcccgc cacgaggcct atcgggacat
      181 ggagaaaaag ggcgtgccca tcacccacac cgacgacgac tcggtgggca tgtgcggcag
      241 ctggtgctct tttgtgagcg accccgaggg caaccagtgg gagttcatgg agttttcacc
      301 ggtcagccag cagatcaagg atctgccgga agacctgaat ttcaaaggct gagggaggaa
      361 cagacaatgg attacttgaa gaggctcacg cactgcacct ttacctgcaa ggactacccc
      421 gccatggtcc gcttttacgg cgagaccctg ggcctgaaaa aggtcttcac gctgccctac
      481 aacgaggaca tcctgcaggg ctttcgcgac aagggctatc agaacgtgac ggcaaagccg
      541 ggggatgagt ggatcagcta tttcgaggtt gcgccgaaag agtttgtcga gctgttcaac
      601 ctgccctaca acggcgagaa cgacacccag gacgagggct ttcaccactt ttgcctgctg
      661 gtggacgaca tcgtggccgc ggcccgcgag ctggagggca aaggggtgac cctgtggaac
      721 ggccccaagt ggatgaacaa ccccctcacc gagccctatc ccgcggaccc catcccggcg
      781 ggcaggcagg gccagtgcgg ctcgctggcc ttttacatcc aggacccgga gggcaatgag
      841 atcgagatca tgcagtacac cccggacagc ctgcagctga aatgtcagaa ggtgaacgga
      901 tgaaaaaggg gtatcagctg ttctccgcct ggaaaaagtg cgccgacgcc gcggggctga
      961 aagacgtggt gcgccgcatc gcggccatgg gctacgacgg ggtggagttc ttctcctacg
     1021 ccggggtgcc ggcggcggag atgaaagcgc tgctggacga gtgcggcatc tgcgcgttca
     1081 actcccacgt gcagctggag cgctggcaga aggacgcgga ttttgagatc gcctacgcca
     1141 gggcgctggg catggaggct ctcaccatct cctacatcgc gccggaggac cgc
//
LOCUS       DBMDYA010000789         1193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21372_length_1193_cov_1.829670, whole genome shotgun sequence.
ACCESSION   DBMDYA010000789 DBMDYA010000000
VERSION     DBMDYA010000789.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21372_length_1193_cov_1.829670"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            945..>1193
                     /locus_tag="ACZPFK_14230"
     CDS             945..>1193
                     /locus_tag="ACZPFK_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407514.1"
                     /translation="MGFGFRLTRCFHVSAVVLPLPTNQKRRTVFLKKEPPQGDGGASL
                     TQKGGIFFQPLRLPPPRAAKADTQESAPLPFFLLTESKR"
BASE COUNT          317 a          317 c          290 g          269 t
ORIGIN      
        1 cctctctccc ccataaagca aaccggacaa aagaaaaaac gactgcgcag gggctggttt
       61 tatgttccat cctgactttt gcgcgcatcc ggcccgtcct gcagccggtg ccgttgtggg
      121 aaaacgccct aaaaagaata aaatggcatc caagtgcaca gacgggagtc tgaaccatcg
      181 caggcaaaag agacggttta acgtttccaa atgaaagcgg agtaataaaa aatagtgcaa
      241 agcactattt tttacgcgca tccgcccact ctgtgggcgg gctctcagat ttcatggcca
      301 cggcggcgaa acgccgccgc atggccgttt tttatagttt gcctatggca aactataaaa
      361 atagcctgcg gctatttttt tggccattcg tttcggttgc cgcgcagcgg cgagaaacag
      421 ggccatagaa ataatttttt tattgcgcag caataaaaga tagccgcagc aaagcagagg
      481 ctatcttttc ggccatgcgg ccagtcctct ggccacggcc ataggaataa gtcgtttttt
      541 attgcaaagc aataaaaaaa ccgcatccga acaaaatcag atgcggccgc gtcccagacc
      601 atggccaatc gggagcgcgg aaaaaagata aggaaagtga aaaaacagga gccctgatgc
      661 aaagaggaaa agatcgagaa tccttttcat cgcgatcatg ggagaaatta gttagtatta
      721 actaacttaa agaaagtata ccatcccttt tatctcctgt caagagggaa actaaaaacc
      781 aatgactcgg ttttgtcctc ctctcttccc tagggcgggg ccgtgtgagc tgcgcacctc
      841 gaaagcgctc tcatcgatac cattcccgcc tttttcgtcc agcgttcttt ctcaacccgc
      901 tccaaaacgg ccgacgtgtg agacctcgcc cggggagggg ggctgtgggc ttcggattcc
      961 gtttgacgcg ttgttttcat gtctccgccg ttgttttgcc tcttccgact aaccaaaaac
     1021 gccgcacggt ttttctgaaa aaggagccgc cgcaaggtga cggaggagcg tccctcaccc
     1081 aaaagggcgg aatctttttt caaccgctcc gcctcccgcc gccgcgtgcg gccaaagcgg
     1141 atactcaaga aagcgcaccg ctgccatttt tccttttgac agagtccaaa aga
//
LOCUS       DBMDYA010000790         1191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21413_length_1191_cov_2.866055, whole genome shotgun sequence.
ACCESSION   DBMDYA010000790 DBMDYA010000000
VERSION     DBMDYA010000790.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21413_length_1191_cov_2.866055"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="ACZPFK_14235"
     CDS             <1..885
                     /locus_tag="ACZPFK_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR1407515.1"
                     /translation="YTKPAAEAAKVVLAQINPRMPRTLGDSFIHVSRLDAVVEADDPI
                     LELQPPAIGEVERSIGKYCADLIRDGDTLQLGIGAIPDAVLLFLKGKKDLGIHSEMFS
                     DGVVELVEAGVVTNARKNLHPGKSVVTFLMGTRRLYDYVNDNPEVEMYPVDYVNDPRV
                     IAQNDNLVSINSCVQVDLMGQVVSTSVGLRQISGVGGQVDFVRGANMSRGGRSIMAMP
                     STAAHGAISKIVPLIDEGAAVTTSRYDVGYVVTEYGIAELRGKTLRQRARALIGIAHP
                     AFRDGLCAEYERRFHEAY"
BASE COUNT          233 a          325 c          395 g          238 t
ORIGIN      
        1 tacaccaaac cggccgccga ggcggccaag gtggtgctgg cgcagatcaa cccgcgcatg
       61 ccgcgcacgc tgggcgactc gtttatccat gtgagccgcc tggacgcggt ggtggaggcg
      121 gacgacccca ttttggagct gcagccgccc gcgatcggcg aggtggagcg gtccatcggc
      181 aagtactgcg ccgacctgat ccgggacggc gacacgctgc agttgggcat cggggccatc
      241 ccggacgccg ttctgctttt tttgaaaggc aaaaaagacc tgggcatcca ctcggagatg
      301 ttctcggacg gggtggtgga actggtggag gcgggcgtcg tcaccaatgc ccgcaaaaac
      361 ctgcaccccg gcaaaagcgt ggtcaccttt ttgatgggca cccgccgcct gtacgactat
      421 gtgaacgaca accccgaggt ggagatgtac ccggtggact acgtcaacga cccgcgcgtc
      481 atcgcccaaa acgacaacct ggtgtcgatc aactcctgcg tgcaggtgga tttgatgggg
      541 caggtggtct ccacctcggt ggggctgcgg cagatcagcg gggtgggcgg ccaggtggat
      601 tttgtgcgcg gggccaatat gagccgcggc ggcaggtcga tcatggcgat gccctctact
      661 gccgcccacg gcgccatcag caagatcgtg cccctgatcg acgagggcgc ggcggtgacg
      721 accagccgct acgatgtggg ctatgtggtc accgagtacg gcatcgccga gctgcggggc
      781 aaaacgctgc ggcagcgggc ccgggcgctg atcggcatcg cgcacccggc ctttcgcgac
      841 ggcctgtgcg ccgagtacga aagacgcttt cacgaggcgt actgaaacgg tgtcggcagc
      901 agagaaaacg agagtgggcc cgccggccgg aaacggacgg cgggcccgtt tcgtctgcgg
      961 ctgtttttat ggccgtgacc cagggatgga ccgcggccat aaaaacagga ttggattttg
     1021 tatacaaata attggtccat tcttttccgt tgctctcatg atattagcaa cagtgagtag
     1081 ggtacatata aaggggaaat ttggaatttg gggattgctt aagggataat ggtgtaatga
     1141 acttttcggt gctctctatt gctgctgttt ttcttggaat tattattttc g
//
LOCUS       DBMDYA010000791         1189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21455_length_1189_cov_1.528493, whole genome shotgun sequence.
ACCESSION   DBMDYA010000791 DBMDYA010000000
VERSION     DBMDYA010000791.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21455_length_1189_cov_1.528493"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="ACZPFK_14240"
     CDS             complement(<1..839)
                     /locus_tag="ACZPFK_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1407516.1"
                     /translation="MRRLTKYLRPYRLQCILGPAAKLVEAVFELIVPLVMAKIIDVGI
                     AAGDKGYVARMSLVLVLLGFTGLCCALFCQYSAARASQGFGTTLRRELYRHINTLSHA
                     EIDRIGSSSMVNRIVSDVNQLQTAVAMLIRLVIRAPFLVIGSTILAVGIDLKLSLIFL
                     TAAPLIAFILYFIMSRCVPYYRTIQKKLDRIGLVTSENLQGVRVIRAFSRQEAERRRF
                     DAANEEYREVSMRVGRVNALLNPLTFVVVNLAIVAIIWAGGWRVQSGALTQGQIVALV
                     NYM"
     gene            966..>1189
                     /locus_tag="ACZPFK_14245"
     CDS             966..>1189
                     /locus_tag="ACZPFK_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1407517.1"
                     /translation="MDLKQLGYFVAIVEEGSISAAAKQLHISQPPLSAQLRRLEEELG
                     VPLIERGPRHVTPTEAGRALYRQAKRLLLLA"
BASE COUNT          232 a          372 c          373 g          212 t
ORIGIN      
        1 ttcatatagt tgaccagggc cacgatctgc ccctgggtca gcgcgccgga ctgcacccgc
       61 cagccgcccg cccagatgat ggccacgatg gccaggttca ccaccacaaa ggtgagcggg
      121 ttcagcagtg cattgacccg gcccacccgc atgctcacct cccggtactc ctcgttggcc
      181 gcgtcaaagc gccggcgctc ggcctcctgc cgcgaaaagg cgcggatgac ccgcaccccc
      241 tgcaggttct cggaggtgac aaggccgatg cggtccagct ttttctggat ggtgcggtag
      301 tagggcacgc agcggctcat gatgaaataa aggataaacg cgatgagcgg ggccgccgtc
      361 aaaaagatca gcgacagctt gaggtcgatg cccacggcca ggatggtgga gccgatcacc
      421 agaaacggcg cccggatgac cagccggatc agcatggcca ccgcggtctg cagctggttg
      481 acgtcgctga cgatgcggtt gaccatgctg ctggagccga tgcggtcgat ctccgcgtgg
      541 gagagggtgt tgatgtggcg gtagagctcg cgccgcagcg tggtgccgaa gccctggctg
      601 gcccgggccg ccgagtactg gcagaacagg gcgcagcaga gcccggtgaa ccccagcagc
      661 accagcacca ggctcatgcg cgccacatag cccttgtccc ccgcggcgat acccacgtcg
      721 atgatctttg ccatcaccag cggcacgatc agctcgaaca ccgcctccac cagcttggcg
      781 gccggcccca ggatgcactg taaccggtag ggccgcaaat attttgtcag tctgcgcatg
      841 acgcagcctc ctttttgtgg atgatcttcc tttacagtgg cattataaca cagtataatg
      901 tatatgaaaa atatttgttt tgtattgtat ccatatttaa aaaatatagt atagggggcg
      961 cggccgtgga cctcaagcag ctgggttatt ttgtcgccat cgtcgaggag ggcagtatca
     1021 gcgccgccgc aaagcagctg cacatctcgc agccgccgct gtcggcccag ctgcggcggc
     1081 tggaggagga gctgggagtg ccgctcatcg agcgcgggcc gcgccacgtc acccccaccg
     1141 aggcggggcg ggcgctctac cgccaggcca agcgtctgct gctgctggc
//
LOCUS       DBMDYA010000792         1178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21686_length_1178_cov_1.641597, whole genome shotgun sequence.
ACCESSION   DBMDYA010000792 DBMDYA010000000
VERSION     DBMDYA010000792.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21686_length_1178_cov_1.641597"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1178)
                     /locus_tag="ACZPFK_14250"
     CDS             complement(<1..>1178)
                     /locus_tag="ACZPFK_14250"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR1407518.1"
                     /translation="CCGEGRGLPQDKARAFQLYERAARQDYAPSVCSLGLCYENGDGV
                     EMDKAHAAELYRRAADLGHPASMCNLGFCYLTGIGVAPDPAQAVPWLEKAAENGSSRA
                     LDLLGDCYSEGRGVARDQVKGFHYYLEGAKQGYLPAQCDAGYCLLEGEGVERDPAGAV
                     AWLEKAAQKEYPRAQSLLARCYKTGDGVEKDFEKALEWYRRSADQGYAPAQCNLGRCY
                     ELGECGLTRDYEEAARLYQLAAEQGDAAAQNNLGHFYYNGYGVEKNYSLAVHWFEKAA
                     AHGLTSALYNLGLCYEKGNGVTRDKAKAAEFYAKAADQGDEDAMFKLGCWHRDGEAGF
                     APDAKEARAYLLRAQKAGHKRAAEELEKLNDGEVSGGAARQERPSSDAGKQSPDRQKK
                     "
BASE COUNT          172 a          428 c          349 g          229 t
ORIGIN      
        1 actttttttg ccggtcgggc gactgcttcc ccgcgtcgga ggagggccgc tcctgccggg
       61 cggcgccgcc ggagacctcg ccgtcgttca gcttttccag ctcctcggcc gcccgcttgt
      121 gccccgcctt ttgggcgcgc agcagatagg cgcgcgcctc ctttgcgtcc ggcgcaaacc
      181 ccgcctcgcc gtccctgtgc cagcagccca gcttgaacat ggcgtcctcg tccccctggt
      241 cggccgcctt ggcataaaac tccgccgctt tggccttgtc ccgggtgacc ccgttcccct
      301 tttcatagca gaggcccaga ttgtacagcg ccgacgtcag gccgtgggcc gccgcctttt
      361 caaaccagtg cacggcaagg ctgtagttct tttcgacacc atagccgttg tagtaaaagt
      421 gccccaaatt gttctgggcc gccgcgtcgc cctgttccgc cgccagctga tacaggcggg
      481 ccgcctcctc ataatcccgg gtgaggccgc attcgcccag ctcatagcag cgccccagat
      541 tgcactgggc cggggcatac ccctggtccg cggacctgcg gtaccattcc agagcctttt
      601 cgaagtcctt ttccaccccg tctccggtct tatagcagcg cgccaacaag ctctgggcgc
      661 ggggatactc cttttgtgcg gccttttcaa gccaggcgac ggcgccggcg gggtctcttt
      721 ccacgccctc cccctccagc agacagtatc cggcgtcgca ctgggcgggc aggtaaccct
      781 gctttgcgcc ctccagataa tagtgaaagc cttttacctg gtcgcgggcc acgccgcgcc
      841 cctcgctgta gcaatcgccc aacaggtcca gcgcgcggga cgagccgttt tcagcggctt
      901 tttccaacca gggcaccgcc tgcgccgggt cgggcgccac cccgatgccg gtgagatagc
      961 aaaagcccaa attgcacatg gaggcgggat gtcccaggtc ggcggcgcgc cggtagagct
     1021 cggccgcatg tgctttgtcc atctccacgc cgtctccgtt ttcgtagcac aggcccaagc
     1081 tgcacaccga gggggcatag tcctgccggg cggcgcgctc atagagctga aaggcgcgcg
     1141 ccttgtcctg gggcaggccg cggccctcgc cgcagcaa
//
LOCUS       DBMDYA010000793         1178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21692_length_1178_cov_1.249768, whole genome shotgun sequence.
ACCESSION   DBMDYA010000793 DBMDYA010000000
VERSION     DBMDYA010000793.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21692_length_1178_cov_1.249768"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="ACZPFK_14255"
     CDS             <1..1047
                     /locus_tag="ACZPFK_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1407519.1"
                     /translation="TLILGELVPKRVAMKDPDAVALKVVGVVWGMYRVCRPFVKFLAL
                     STNLVLKLFGIGAANEEEPVDEEDILMMVEAGEEAGALEPHERAMIENIFEFDDLHVS
                     EVMTHRTDIAAVERTAPLAALVELQREQRFSRLPVYEGDLDNIVGIVYVKDILPALGG
                     GREAGKTAADFMRPVPYVPESMRCSGLLRQFKERRQRMAIVVDEYGGTEGLVTMEDLL
                     ESIVGKLDDEHDEGAGIVQLSEDSWMLDGELEIDEVEQLLGSDLFENDDSDTLNGFIT
                     NMLGRIPAQGEVIPLKTDGWLCTILAANQRCIERLKIERAAGAGREDAAEQAESGKKE
                     KTKKSDKKRDREER"
BASE COUNT          230 a          341 c          437 g          170 t
ORIGIN      
        1 accctgatcc tgggcgagct ggtgcccaag cgcgtggcca tgaaagaccc ggacgccgtc
       61 gccctcaagg tggtgggggt ggtctggggt atgtaccggg tctgccggcc tttcgtcaaa
      121 tttttggccc tttccaccaa cctggtgctg aagctgttcg gcatcggcgc cgccaatgag
      181 gaggagccgg tggacgagga agatatcctg atgatggtgg aggccggcga ggaggccggc
      241 gcgctggagc cgcacgagcg ggccatgatc gagaacatct ttgagttcga cgacctgcac
      301 gtcagcgagg tcatgaccca ccgcaccgac atcgcggcgg tggagcgcac cgccccgctg
      361 gcggcgctgg tggagctgca gcgggagcag cgcttctccc gcctgccggt gtacgagggg
      421 gatctcgaca acatcgtcgg catcgtctat gtcaaggaca tcctgcccgc cctgggcggc
      481 ggccgggagg cgggtaagac ggcggccgac tttatgcggc cggtgcccta tgtgcccgag
      541 agcatgcgct gcagcggcct gctgcgccag ttcaaagagc gtcgccagcg catggccatc
      601 gtggtggacg agtacggcgg caccgagggg ctggtgacca tggaggacct gctggaatcc
      661 atcgtgggca agctcgacga cgagcacgac gagggggcgg gcatcgtcca gctgtccgag
      721 gacagctgga tgctggacgg cgagctggag atcgacgagg tggagcagct gctgggcagc
      781 gacctgtttg aaaacgacga ctcggacacc ctgaacggct ttatcaccaa catgctgggc
      841 cgcatcccgg cccagggcga ggtcatcccg ctgaagaccg acggttggct gtgcaccatc
      901 ctggccgcga accagcgctg catcgagcgg ctgaagatcg agcgggcggc cggggcgggc
      961 cgggaggacg cggccgagca ggcggaaagc ggcaaaaagg aaaagacgaa aaagagcgat
     1021 aaaaaaagag acagggagga gcgatgagat gacggcggca gagcggcttt tgaggtatgt
     1081 ggcggtggcc accgacagcg atgaggacag cgcgtccacc ccctccagcg cccgccagtg
     1141 ggacctggcc cgcatgctgg aggaagagat gcgcggcc
//
LOCUS       DBMDYA010000794         1175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21761_length_1175_cov_1.374302, whole genome shotgun sequence.
ACCESSION   DBMDYA010000794 DBMDYA010000000
VERSION     DBMDYA010000794.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21761_length_1175_cov_1.374302"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="ACZPFK_14260"
     CDS             <1..195
                     /locus_tag="ACZPFK_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407520.1"
                     /translation="ELPCRELVPAIARQEQATDAIFERPGRMMKLSLVSLACTQNAVI
                     TDKGLFDGRTQRFYEQFPQA"
     gene            410..1111
                     /locus_tag="ACZPFK_14265"
     CDS             410..1111
                     /locus_tag="ACZPFK_14265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1407521.1"
                     /translation="MEKRAFDIGGIPTLLWGPASERLILAVHGSGSHKADVPIRLLAE
                     DAVPKGYQILSFDLPRHGARAQDPTPCKVQPCVEELTAVLAYIRPRWPRRSVFGNSLG
                     AYFSLLACRDEPLQQALFLSPVVDMRRLIENMMGWAGVTPERLQAEGEIATDFGETLD
                     WSYYCYVCGHPVDRWQTPTDILYGGRDALCEGEVVRAFAQRFGCGLETVPWAEHYFHT
                     PRELDALRAWFAQKL"
BASE COUNT          211 a          345 c          423 g          196 t
ORIGIN      
        1 gagctgccct gccgcgagct ggtgcctgcc atcgcccggc aagagcaggc caccgacgcg
       61 atctttgagc gcccgggccg catgatgaag ctctcgctgg tctcgctggc ctgcacccag
      121 aacgcggtca ttaccgacaa gggcttgttc gacggcagga cacagcggtt ctacgagcaa
      181 tttccgcagg cttgaacgga aagccgggat aggcggggcg gagaaaatcc cgggccccgt
      241 gaaacggcgg agaaaagacg cgcggcgggt gccgcttgcc cctatcctcc cccggcagag
      301 aggggggcga agctgtcggc ccggcaggcg cgtcttcaaa aaatgaaaaa tacggcctgg
      361 aggacaggag aaaaagccgc accgggggcg gtgcggcttt taggtgaaaa tggaaaagag
      421 agcgtttgac atcggcggca tcccgacgct gctctggggc cccgcctcgg agcggctgat
      481 tttagccgtg cacggcagcg gttcgcacaa agcggacgtg ccgatccggc tgctggccga
      541 ggatgcggtg cccaaggggt atcagatact cagctttgac ctgccgcggc acggggcgcg
      601 ggcgcaggac cccaccccct gcaaggtgca gccttgcgtg gaggagctga cggccgtgct
      661 ggcctatatc cggccgcgct ggccgcggcg cagcgtgttc ggcaacagcc tgggggccta
      721 tttcagcctg ttggcctgcc gggacgagcc gctgcagcaa gcgctgttcc tctccccggt
      781 ggtggatatg cggcggctca tcgagaatat gatgggctgg gccggtgtga cgccggagcg
      841 gctgcaggcc gagggggaga tcgccaccga ttttggggaa acgctggatt ggagctatta
      901 ttgctatgtc tgcgggcatc ccgtcgaccg gtggcaaacc cccaccgata ttttgtacgg
      961 cggcagggac gccctctgtg agggggaggt cgtgcgggct tttgcacagc ggtttggctg
     1021 tgggctggaa accgtgccct gggcggaaca ctactttcac acgccgcggg agctggacgc
     1081 cctgcgggcg tggttcgccc aaaagctctg agcgcccggc gcgggcggga ggcggagata
     1141 aaaaatagcc gcaggctatt ttttcggcca tccgg
//
LOCUS       DBMDYA010000795         1173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21804_length_1173_cov_0.992537, whole genome shotgun sequence.
ACCESSION   DBMDYA010000795 DBMDYA010000000
VERSION     DBMDYA010000795.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21804_length_1173_cov_0.992537"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="neuC"
                     /locus_tag="ACZPFK_14270"
     CDS             complement(<1..895)
                     /gene="neuC"
                     /locus_tag="ACZPFK_14270"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIR1407522.1"
                     /translation="MSGAKKRRVAVVTGTRAEYGLLRPVCQKLLASDELSLELVVTGA
                     HLSEKYGNTVTEIEADGLPIAARIPILNHPSTALGTCRTIGDAVGLFAEQFSASRPDC
                     VLVLGDRYEVFAAATAAAVLRIPLAHISGGDVTAGAADEFFRHSITKMASVHFPSCSQ
                     YAARIIRMGEEPQRVFNVGGLGDENIRTMALLTREELGRAFDFDFTKPYLLVTYHPVT
                     LGGDPAAEVAALLEALDAFPEMGVLFTKANADAGGEEINALLDRYAASRQNAVAFSSM
                     GAKGYLSAMGCCAAVVGNSSSG"
     gene            complement(892..>1173)
                     /locus_tag="ACZPFK_14275"
     CDS             complement(892..>1173)
                     /locus_tag="ACZPFK_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR1407523.1"
                     /translation="LSPSELMEMVSAIRRTEAMLGSGHKGVSFSERKNIAIARKSIVA
                     ARDIAPGEAFTEENLTVKRPGQGVSPMRWYEVLGKRAKRAFAEDEMIEV"
BASE COUNT          190 a          403 c          394 g          186 t
ORIGIN      
        1 cgccggagga ggaattgccc accaccgccg cgcagcagcc catggccgag aggtacccct
       61 tggcccccat cgaggaaaag gccacggcgt tttgccgcga ggcggcatag cggtccagca
      121 gggcgttgat ctcttcgccc ccggcgtcgg cgttggcctt ggtgaagagt actcccatct
      181 cggggaaagc gtccagcgcc tccagcagcg ccgccacctc cgccgccggg tcgcccccca
      241 gcgtcaccgg gtgataggtg accagcagat aaggctttgt gaaatcaaaa tcgaaagcgc
      301 gccccagctc ctcgcgggtg agcagggcca tggtgcggat gttctcatcc cccaggccgc
      361 ccacgttgaa gacccgctgc ggctcctccc ccatgcggat gatgcgggcg gcatactggg
      421 agcaggaggg aaaatgcacc gaggccatct tggtgatgga gtggcggaag aactcgtccg
      481 ccgcgcccgc cgtcacgtcg ccgccgctga tgtgggccag cgggatgcgc agcaccgccg
      541 ccgccgtggc cgcggcgaag acctcatacc ggtcccccag caccagcacg cagtccggcc
      601 gggaggcgct gaactgctcc gcaaacagcc ccaccgcgtc gccgatggtg cggcaggtgc
      661 ccagggcggt ggagggatgg ttcaaaatgg ggatgcgggc ggcgatgggc aggccgtccg
      721 cctcgatctc ggtcaccgta ttgccgtatt tttccgacag gtgggcgccg gtcaccacca
      781 gctccaggga cagctcgtcc gaggccagca gcttctggca gaccgggcgc agcaggccgt
      841 attcggcgcg ggtgccggtg accaccgcga cgcggcgctt ttttgccccg ctcatacttc
      901 gatcatctcg tcctcggcaa aggcgcgctt tgcgcgcttg ccgagcacct cgtaccagcg
      961 catgggcgaa acgccctgcc ccggccgttt cacggtcaga ttctcctcgg tgaacgcctc
     1021 gccgggggcg atgtcccggg ccgccacgat gctcttgcgg gcgatggcga tgtttttgcg
     1081 ctcggaaaag ctgaccccct tgtgcccgct gcccagcatg gcctcggtgc ggcggatggc
     1141 ggagaccatc tccatcagct cggagggaga gag
//
LOCUS       DBMDYA010000796         1172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21821_length_1172_cov_1.370682, whole genome shotgun sequence.
ACCESSION   DBMDYA010000796 DBMDYA010000000
VERSION     DBMDYA010000796.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21821_length_1172_cov_1.370682"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(192..725)
                     /locus_tag="ACZPFK_14280"
     CDS             complement(192..725)
                     /locus_tag="ACZPFK_14280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR1407524.1"
                     /translation="MANIWHDISPARINPEDFIAVIEIEKGSKNKYELDKETGHIILD
                     RILYTSTHYPANYGLIPRTYADDGDPLDVLVVCSESIRPLSLVRCYPIGVMAMLDSGK
                     MDEKIIAIPFEDPTYNEYRDINELPSHVFSEMRHFFTVYKSLEGKETVVNDVSGAEEA
                     KKVIQNCLDAYIEKFCR"
     gene            complement(895..>1172)
                     /locus_tag="ACZPFK_14285"
     CDS             complement(895..>1172)
                     /locus_tag="ACZPFK_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1407525.1"
                     /translation="LQKEGMSRPRAFVYGALSGIVEPLGGLVGVLLAVTMAKVMPFML
                     SFAAGAMIYVVVEELVPEASGEGHGHAGVIGSMLGFALMMLLDVALG"
BASE COUNT          217 a          393 c          321 g          241 t
ORIGIN      
        1 cagggtctca agataggcgt cgaaccgctg ctgctccgtc tcccctgtct gggatccgcc
       61 gcaggcggcc agcgagagcg ccatccccag cgccagggca aagctcaaaa tccgtttcat
      121 ccgattctct cctctttcaa aaacagacaa aagcggcgcc cccggccccg cttttgcccc
      181 tctatttccc gttatcggca gaacttttca atgtaggcgt ccaggcagtt ctggatcacc
      241 tttttggcct cctctgcgcc ggagacgtcg ttgaccaccg tctccttgcc ctccaggctc
      301 ttgtagacgg taaaaaagtg ccgcatctcg ctgaacacgt ggctgggcag ttcgttgatg
      361 tcgcggtact cgttgtaggt ggggtcctca aaggggatgg cgatgatctt ttcgtccatc
      421 ttgcccgaat ccagcatcgc catcaccccg atgggatagc agcgcaccag gctgaggggg
      481 cggatgctct cgctgcacac caccagcacg tccagcgggt cgccgtcatc cgcataggtg
      541 cgcgggatga ggccgtagtt tgccgggtaa tgggtggagg tgtaaaggat gcggtccagg
      601 atgatgtgac ccgtctcctt gtccagttca tatttgttct tcgagccctt ttcgatctcg
      661 atgactgcga taaaatcctc gggattgata cgggcggggc tgatatcatg ccagatattc
      721 gccataaagc gtcccttcct ttcctgtacc aacccagtat accatgaagc tggcgcggaa
      781 ggaagttttt tccgtcccca tgccggcgcg cccggcgtgg acaggcgccc tgtgcccctt
      841 gcccctcctg ccgggagggg ggatcgcaaa gcgcctcacc gcgccgcccg gctttcagcc
      901 cagcgccaca tccagcagca tcatcagcgc aaagcccagc atggagccga tgactccggc
      961 atggccgtgc ccctcgccgc tcgcctcagg caccagctct tccaccacca catagatcat
     1021 ggcccccgcc gcaaaggaga gcatgaaagg catcaccttt gccatggtga ccgccagcag
     1081 cactcccacc aggcctccca gcggctccac tatgccggac agcgccccat agacgaacgc
     1141 gcgcgggcgg ctcatgccct ctttttgcag cg
//
LOCUS       DBMDYA010000797         1165 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_21989_length_1165_cov_1.300752, whole genome shotgun sequence.
ACCESSION   DBMDYA010000797 DBMDYA010000000
VERSION     DBMDYA010000797.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21989_length_1165_cov_1.300752"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="ACZPFK_14290"
     CDS             complement(<1..542)
                     /locus_tag="ACZPFK_14290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR1407526.1"
                     /translation="MTLLNGTGLAAWVLFYLIYFVKNALLRRSGIRANRIGKGEKPRA
                     AARVERLLGPVTLTLGVLQPLSLWPGLLQPLSLPDAARWAGILLALVGDGVFLLSVYR
                     MGASWRAGVDATQRTALVTDGIYAHSRNPAFLGFDLLYLGLALAAPNPPHLLLAALGV
                     LTLHLQILQEEAYLSAACGDA"
     gene            complement(892..>1165)
                     /locus_tag="ACZPFK_14295"
     CDS             complement(892..>1165)
                     /locus_tag="ACZPFK_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1407527.1"
                     /translation="GGLGSDAAIEAADIVLMDDKPSELVVAIRIAKKTLGIVRQNIVF
                     ALAVKLAVLVLSALGMAGMWEAVFADVGVSVRAILNASRALNTKKL"
BASE COUNT          220 a          403 c          361 g          181 t
ORIGIN      
        1 gcgtccccgc aggccgccga gaggtaggct tcctcctgca aaatttgcag atgcagggtc
       61 aggaccccca gtgcggccaa aaggagatgc ggcgggttgg gggcggccag agccaacccc
      121 agataaagca ggtcaaagcc cagaaaggcc gggttgcggc tgtgggcgta gatgccgtcg
      181 gtcaccagcg cggtgcgctg ggtcgcgtcc acaccggcgc gccagctcgc ccccatccgg
      241 tacactgaga gcaaaaacac cccatccccc acaagggcca gcagaatgcc cgcccagcgc
      301 gccgcgtccg gcagggacag gggttgcagc aggcccggcc aaaggctcaa gggctgcaaa
      361 acacccagcg tcagggtcac cgggccgagc aggcgctcca cccgggccgc cgcgcgaggc
      421 ttttccccct tgccgatgcg gttcgcccgg atgccgctgc ggcgcaaaag tgcgtttttc
      481 acaaaataaa tgagataaaa cagcacccag gccgccagcc ccgtcccgtt cagcagcgtc
      541 atgaaaaccg cttcctttcc ccttttgatc ctctttgcag aaaaaacagg ccgccgcgtc
      601 gatttgcctt tcagcccttt tcgcgcagcg gcccgccttt tatctcttgt ggggcaaagc
      661 cgcactcccc cggccttgcc ccgcaaggcc tttcctttcc ctggcccggc gtgtttgccc
      721 gaaagggcaa aaccgcacag cgtcggccgc gcgtggaaaa ggtgcggctc tgtgagaaag
      781 cggcgcgccg cgtcccatac gactcgcagg gagcgccggg gcggcggacc ggccttttcc
      841 gccagaatca ggcgacaggt ttcccggtca aagccgcggt gaaaatgggc ctcagagctt
      901 tttggtgttg agcgcgcggg aggcgttgag gatggcgcgc accgaaacgc ccacgtcggc
      961 aaagaccgcc tcccacatgc ccgccatgcc cagcgcggag agcaccagaa ccgccagctt
     1021 gacggccagg gcaaagacga tgttctgccg cacgatgccc agtgtttttt tcgcgatgcg
     1081 gatcgccacc accagctcgg agggcttgtc gtccatcagc acgatgtccg ccgcctcgat
     1141 ggcggcgtcg ctgcccaggc cgccc
//
LOCUS       DBMDYA010000798         1164 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22022_length_1164_cov_1.206961, whole genome shotgun sequence.
ACCESSION   DBMDYA010000798 DBMDYA010000000
VERSION     DBMDYA010000798.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22022_length_1164_cov_1.206961"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="ACZPFK_14300"
     CDS             complement(<1..1135)
                     /locus_tag="ACZPFK_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR1407528.1"
                     /translation="MKDGFIKVAAASPKLRVADCDYNLGEILKAVRRAADLGAQVLVL
                     PELCLTGYTCGDLFFQDALLDGARRALEELARETRGLDLLIVAGLPWRENGKLYNCAA
                     VLCRGEVLGLVPKVNLPNYGEFYERRHFTPAFEGVRSAWVAGCEAPFGAGLLFRCREL
                     PALCLGVEICEDLWVPAPPSVRLALGGATVIVNPSASDETTGKCAYRRTLVCSHSARL
                     LCGYVYADAGAGESTGDLVFAGHNLIAENGALLAESPLFGEGVTVSEIDVGRLIGERA
                     RTTTFAAGQDEGLRTVQFSLTPRETALTRPIPPHPFVPEDEAERARRCEEIFSIQAAG
                     LCKRVEHTGCGSLVIGISGGLDSCLALLVAVRALNRLRRPRTDI"
BASE COUNT          188 a          401 c          412 g          163 t
ORIGIN      
        1 taatgtcggt gcggggcctg cgcagccggt tgagggcgcg caccgccacc agcagggcga
       61 ggcagctgtc cagcccgccg ctgatgccga tgaccagcga gccgcagccc gtgtgctcga
      121 cccgcttgca gaggccggcc gcctggatgg agaagatctc ctcgcagcgg cgcgcccgct
      181 cggcctcgtc ctcgggcaca aagggatgag gggggatggg gcgggtgagc gccgtctccc
      241 gcggggtgag actgaactgc accgtgcgca gaccctcgtc ctgcccggcg gcaaaggtgg
      301 tggtgcgggc ccgctcgccg atgaggcggc ccacgtcgat ctcgctcacc gtgaccccct
      361 cgccgaacag cgggctctcg gcgagcaggg cgccgttttc cgcgatcagg ttgtgcccgg
      421 caaagaccag gtccccggtg gattccccag cgcccgcgtc cgcgtagacg tagccgcaca
      481 gcagacgggc ggagtgggag cagaccaggg tgcggcggta ggcgcatttg ccggtcgtct
      541 cgtcgctggc ggaggggttg acgatgacgg tggcgccgcc cagggccagc cgcaccgagg
      601 ggggcgccgg cacccagagg tcctcgcaga tctccacccc caggcaaagg gcgggcagct
      661 cgcggcagcg aaacagcagc cccgcgccga aaggcgcctc gcaccccgcg acccaggcgc
      721 tgcgcacccc ctcaaaggcg ggggtgaagt gacggcgctc ataaaactcg ccgtagttgg
      781 gcaggttgac cttgggcacc aggcccagca cctcgccgcg gcagagcacg gcggcgcagt
      841 tgtagagctt gccgttttcg cgccagggca gcccggcgac gatcagcagg tcgagaccgc
      901 gggtctcccg cgccagctct tccagcgcgc ggcgcgcgcc gtccagcagc gcatcctgaa
      961 aaaagagatc gccgcaggtg tagccggtca gacagagctc gggcagcacc agtacctgcg
     1021 cgcccaggtc ggctgcgcgg cgcacggcct ttaaaatttc gcccaggttg taatcacagt
     1081 cggcgacccg cagctttggg ctcgcggcgg cgaccttgat gaaaccgtct ttcatttttt
     1141 gttcctcccg ccgatcagaa tgcg
//
LOCUS       DBMDYA010000799         1163 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22044_length_1163_cov_1.187382, whole genome shotgun sequence.
ACCESSION   DBMDYA010000799 DBMDYA010000000
VERSION     DBMDYA010000799.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_1163_cov_1.187382"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="ACZPFK_14305"
     CDS             complement(<1..400)
                     /locus_tag="ACZPFK_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407529.1"
                     /translation="MANGTKDEGLAAAPAAEEKIELAEQGAAARPQRPKKKRNVKKIV
                     LVSALVLAVGAFAVSRVAAMLRGPLPTYAPTQPVAVGDVTQTLSTTGTLVSGNRVTVL
                     SPVSAPLSEVLVEQGQIAPEEAKTHPKRNTI"
     gene            complement(400..1137)
                     /locus_tag="ACZPFK_14310"
     CDS             complement(400..1137)
                     /locus_tag="ACZPFK_14310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1407530.1"
                     /translation="MKRNHRKGGGALPTPIIEIRDLYKIYNPGENEVRALDGVSLEVF
                     EGEFVAIVGHSGSGKSTLMNMIGCLDVPTAGSYRLNGQDVSELSDNELSEIRNREIGF
                     IFQSFNLIKNLNALENVELPLIYRGLPAGRRRELAVRALERVGLGGRMNHRPNQMSGG
                     QQQRVAVARAIATHPPLILADEPTGNLDTKAGARVFEIIRQLHEEGNTVVLITHDEGL
                     AKKAGRVVRITDGKIDDGQAPGGKEIF"
BASE COUNT          159 a          417 c          343 g          244 t
ORIGIN      
        1 tgatggtgtt gcgcttgggg tgggtcttgg cctcttccgg ggcgatctgc ccctgctcca
       61 ccagcacctc gctcagcggc gccgagaccg gcgagagcac cgtcacccgg ttgcccgaaa
      121 ccaaggtgcc ggtggtggag agcgtctggg tgacgtcgcc cacagcgacg ggctgggtgg
      181 gggcataggt cggcagcggc ccgcgcagca tcgccgcgac ccggctcacc gcaaacgccc
      241 ccaccgccag caccagcgcc gataccagca cgatcttttt gacgttgcgc ttctttttgg
      301 ggcgctgcgg ccgggccgcc gccccctgct ccgccagctc tatcttctcc tcggcggcgg
      361 gcgccgccgc cagcccctcg tcctttgtcc cgtttgccat caaaaaatct cctttccccc
      421 gggtgcttgc ccgtcgtcga tcttcccgtc ggtgatgcgc acgacgcgcc cggccttttt
      481 tgccaggccc tcgtcatggg tgatcagcac caccgtgttt ccctcttcat gcagctgccg
      541 gatgatctca aacacccgcg ccccggcctt ggtgtccagg ttgccggtgg gctcgtcggc
      601 caggatcagc ggcgggtggg tggcgatggc ccgcgccacc gccacccgct gctgctggcc
      661 gccggacatc tggttggggc ggtggttcat ccgccccccc aggcccaccc gctccagcgc
      721 gcgcaccgcc agctcccgcc gtctgcccgc aggcaggccc cgatagatca ggggcagctc
      781 cacattctcc agcgcgttga ggtttttgat caggttgaag ctctgaaaga tgaagccgat
      841 ctcccggttg cggatctcgg acagctcgtt gtcgctcagc tcgctcacat cctgtccgtt
      901 cagccggtag ctgcccgccg tgggcacgtc caggcagccg atcatgttca tcagcgtgga
      961 tttgccgctg cccgaatgcc cgacgatggc gacgaactcc ccttcgaata cctccaggct
     1021 gacgccgtcc agggcgcgca cctcgttttc tcccgggtta taaattttgt acaggtcccg
     1081 tatctcgatg atcggtgtgg gcaatgcccc gccccccttt ctatggtttc gcttcatgta
     1141 ttactgtact aattgcataa ttc
//
LOCUS       DBMDYA010000800         1163 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22049_length_1163_cov_1.087571, whole genome shotgun sequence.
ACCESSION   DBMDYA010000800 DBMDYA010000000
VERSION     DBMDYA010000800.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22049_length_1163_cov_1.087571"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="ACZPFK_14315"
     CDS             complement(<1..348)
                     /locus_tag="ACZPFK_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407531.1"
                     /translation="MLQFSGLTLPLSSTEEDAFARALARAGLSAAQVADWGVTRLSVD
                     ARHGRPKLVWGIGLTLAPGLPEAAFARGGAVVKTEAPLLLERGQRRSQVRPVVCGFGP
                     AGIFAALLLARSGA"
     gene            complement(402..>1163)
                     /locus_tag="ACZPFK_14320"
     CDS             complement(402..>1163)
                     /locus_tag="ACZPFK_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1407532.1"
                     /translation="GEVTLTGPEEEIRSVGRVVAKVEIGEELTSTRIATAGLTIYDKD
                     GNVMNSKVITVDAESVEVTVPILQKKSLPVTIGYTNVPEGFDVSLLKPSFSVGSINLA
                     GPADSIQNKQELSLGYIDLQREFALGFAKEFPVTLDTGYENLDNLKQITVSFDTSAFS
                     QKQVTVDEIRVVNQPQNRTVTVNTKRIHDVTILGLTAEVEALTGKSVVAEIDAGDLTL
                     GEGEQTVPVTMKIPGSSTVFAVGSYTVLVEINAKG"
BASE COUNT          176 a          414 c          361 g          212 t
ORIGIN      
        1 cgcgccgctg cgcgccagca gcagggccgc aaagatgccg gcaggcccaa agccgcagac
       61 caccggccgc acctgcgacc ggcgctgtcc ccgctcaagg aggaggggcg cctctgtttt
      121 gaccaccgcc ccgccgcggg caaaggccgc ctccggcagg ccgggcgcca gggtcagccc
      181 gatcccccac accagcttcg gccgcccgtg gcgggcgtcc accgacaggc gggtgacgcc
      241 ccagtccgcc acctgcgcgg cgctgaggcc tgcgcgggcc agggcccggg caaaggcgtc
      301 ctcctctgtg gaggaaaggg gcagcgtcag ccccgaaaat tgcagcatgg cgccgccccc
      361 tctctgatct ctcttccatt ttcaccgccc gtcggggcgg ctcatccctt ggcgttgatc
      421 tccaccagca ccgtatagct gcccaccgcg aagacggtgg agctgcccgg aatcttcatg
      481 gtcaccggca cggtctgctc gccctcgccc agggtcaggt cgcccgcgtc gatctccgcg
      541 acgacgctct tgcccgtcag ggcttccacc tcggcggtga ggccgaggat ggtgacgtcg
      601 tggatgcgct tggtgttcac cgtgaccgtg cggttctgcg gctggttgac cacccggatc
      661 tcgtccaccg tcacctgctt ttgcgaaaac gcgctggtgt cgaagctgac cgtgatctgt
      721 ttgaggttat ccaggttctc atagccggtg tccagcgtca ccgggaactc cttggcaaag
      781 cccagggcaa actccctttg caggtcgata tatcccagcg agagctcctg tttgttctgg
      841 atggaatcag ccggccccgc cagattgatc gaccccaccg agaagctggg cttgagcaga
      901 ctgacgtcaa agccctcggg cacgttggtg tagccgatcg tcaccggcag gctctttttc
      961 tgcagaatgg gcacggtcac ctccaccgac tcggcatcca cggtgatgac cttgctgttc
     1021 atgacgttgc cgtccttgtc gtagatggtc agcccggcgg tggcgatgcg ggtgctggtc
     1081 agctcctcgc cgatctccac ctttgccacc acccggccca ccgagcggat ctcctcctcg
     1141 gggccggtga gggtgacctc ccc
//
LOCUS       DBMDYA010000801         1156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22200_length_1156_cov_1.960190, whole genome shotgun sequence.
ACCESSION   DBMDYA010000801 DBMDYA010000000
VERSION     DBMDYA010000801.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22200_length_1156_cov_1.960190"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            304..1149
                     /locus_tag="ACZPFK_14325"
     CDS             304..1149
                     /locus_tag="ACZPFK_14325"
                     /inference="COORDINATES: protein motif:HMM:NF021592.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HIR1407533.1"
                     /translation="MSLPTAGSIPALSIAFSALSSCIAVLLPVALLVFCRLRYGRAFL
                     AAGVGALCFFLGAMVLERMCHQLVFSLFPTLPYLPAAYALYGCLAAGIFEETARLGGL
                     WLLCRRQPGFATGLAYGIGHGGLESIALVGVSLIGNTVLMLQVNAGGAQALVASVPAA
                     QQQLAAAQLNALAEAAPTSFLAAGAERAIALATQILLSLLVWMVVAGRLPKRFFFLSI
                     ALHALTDVGAVLYQLGLVPNVWLAELWALASCLLVGALALSLLRRTGALPPKAQKGAG
                     AGGQG"
BASE COUNT          129 a          397 c          395 g          235 t
ORIGIN      
        1 ccgaacggca cgggacggtg ccgggagaca ttgggtggca ggagggcgaa gcgccctcgg
       61 atggaatgtg ggggcggcaa agggctgcct cccttttgcc gccgtaacac aggcactttt
      121 ttcgccgcgg gggacagcgc ctcttttacc gcggcgcgcc ggacgccctg cggcgcacgc
      181 cccgggcggg cggtcgccgc ggccacgtcc ccggcgcatc ccccacaggg ttgacagccg
      241 ttcttgggcg cggtacaatg gccgcagatt atttagacgt tcttcaaaat gaggtgtgtt
      301 gtcatgtctc tgcccactgc cggctccatc cctgccctca gcatcgcctt ttccgcgctc
      361 agcagctgca tcgccgtgct gctgcccgtc gcgctgctgg ttttctgccg cttgcggtac
      421 ggccgcgcct ttttggcagc aggcgtgggg gcgctctgtt tttttctcgg ggccatggtg
      481 ctggagcgga tgtgccatca gctggtcttc tctctttttc ccaccctgcc ctatctgccg
      541 gccgcctatg ccctgtacgg ctgccttgcc gccggtattt ttgaggagac ggcgcggctg
      601 gggggccttt ggctgctctg ccgccggcag cccggctttg ccaccgggct cgcttacggc
      661 atcggccacg ggggccttga atccatcgcg ctggtgggcg tctccctgat cggcaacacc
      721 gttttgatgc tgcaggtcaa tgccggcggg gcgcaggccc tggtcgcctc tgtgcccgcc
      781 gctcagcagc agctggccgc ggcgcagctg aacgcgctgg cggaggccgc gcccacctcc
      841 tttctcgccg cgggggcgga gcgggcgatc gccctcgcca cccagattct gctctcgctg
      901 ctggtctgga tggtcgtggc ggggcggctg cccaaacgct ttttcttcct ctccatcgca
      961 ctgcacgccc tgaccgacgt gggcgcggtg ctctatcagc tgggcctggt ccccaacgtg
     1021 tggctggcag agctctgggc tctggccagc tgcctgctgg tgggtgcgct ggcgctctct
     1081 ctgctgcgcc gcaccggcgc gctgccgccc aaggcgcaaa agggcgccgg ggccggcggg
     1141 caggggtaaa aacgcg
//
LOCUS       DBMDYA010000802         1156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22203_length_1156_cov_1.242654, whole genome shotgun sequence.
ACCESSION   DBMDYA010000802 DBMDYA010000000
VERSION     DBMDYA010000802.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22203_length_1156_cov_1.242654"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="ACZPFK_14330"
     CDS             <1..325
                     /locus_tag="ACZPFK_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1407534.1"
                     /translation="PVLDGRGLLRSGVIVRHLVLPGHRDDSLRLLRWLAAALPRDGFI
                     LSLMSQYTPYRPLPYKELNRRLSTFEYEAVVEEALRLGFTRAYTQQRTAAKEEYTPPF
                     DLTGL"
     gene            342..>1156
                     /locus_tag="ACZPFK_14335"
     CDS             342..>1156
                     /locus_tag="ACZPFK_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407535.1"
                     /translation="MSYLYAALYLALFVGTGVLLAGLGLPREPLAARVAVGLSLSVVL
                     QAWLPALPALFLGFRLPSVLVGAALCAALFLVGLRLRPREAARGRFGPAFWACVLPML
                     ALGCALLFTHTLSPRADGSLWAGQSTYGDLPMHLAFIKSLAENGVFPPVYTLVAGDLP
                     MSYPFLCETVSSALLLLGLPLRAACLAPQFIALAAVFCGAYELLRQVFDHAGKAALAF
                     YLFFMGSGFGFFYFLGGRGNFSRIFTAFYETPTNYVAENIVWVNPIVDLLVPQR"
BASE COUNT          139 a          404 c          366 g          247 t
ORIGIN      
        1 gccggtgctg gacgggcgcg gcctgctgcg cagcggcgtc atcgtgcggc acctggtgct
       61 gcctggccac cgggacgaca gcctgcgcct gctgcgctgg cttgccgcgg ccctgccgcg
      121 ggatgggttt atcctcagcc tgatgagcca gtacacgccc taccggccgc tgccctataa
      181 agagctgaac cgccgcctct ccacctttga atacgaagcc gtggtggagg aggccctgcg
      241 cctgggcttt acgcgggcct acacgcagca gcgcaccgcc gccaaagagg aatacacccc
      301 gccctttgac ctgaccggcc tgtgagagga ggaaccacag cttgagctat ctgtatgcgg
      361 ccctgtatct ggcgcttttc gtgggcaccg gcgttctgct ggcaggcctg ggcctacccc
      421 gcgaacccct ggcggcacgg gtcgcggtgg ggctgtcgct ctcggtggtg ctgcaggcct
      481 ggctgccggc gctgcccgcc ctttttctcg gcttccgcct gcccagtgtg ctggtggggg
      541 ccgccctctg cgcagccctt tttctggtgg ggctgcgcct gcggccccgc gaggcggcgc
      601 gcggccgctt tggccccgca ttttgggcct gcgtgctgcc gatgctggca ctgggctgcg
      661 cgctgctgtt cacccacacc ctctcgccgc gggcggacgg cagcctgtgg gcagggcaat
      721 ccacctacgg cgatctgccc atgcacctcg cttttatcaa gagccttgcg gaaaacggcg
      781 tttttccccc ggtctacaca ctggtggcgg gcgatctgcc catgtcgtat ccctttctct
      841 gcgagaccgt ctcctccgcc ctgctgctgc tcggcctgcc cctgcgcgcc gcctgcctgg
      901 cgccgcagtt catcgccctt gcggccgtct tctgcggcgc gtatgagctg ctgcggcagg
      961 ttttcgacca tgcgggaaaa gccgccctgg ccttttatct cttttttatg ggcagcggct
     1021 ttgggttttt ctattttctc ggcggcaggg gcaacttcag ccgcatcttt accgcgtttt
     1081 acgagacgcc caccaactat gtggcggaaa acatcgtctg ggtcaacccc atcgtggatc
     1141 tgctggtgcc ccagcg
//
LOCUS       DBMDYA010000803         1152 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22305_length_1152_cov_1.333968, whole genome shotgun sequence.
ACCESSION   DBMDYA010000803 DBMDYA010000000
VERSION     DBMDYA010000803.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22305_length_1152_cov_1.333968"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="ACZPFK_14340"
     CDS             <1..>1152
                     /locus_tag="ACZPFK_14340"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1407536.1"
                     /translation="QKTDVFKDVRHVEFDKGMQEGIIEYISSEVIERYYDHVKDRSVR
                     PSVCRGSGLKLVYTPLNGTGNLPVRRILADIGIEDVTVVPEQELPDGNFTTCPYPNPE
                     IKQALQLGLDLAKKTGADLLLATDPDADRVGIAVRDGGDYLLLSGNEVGVLLLDYICK
                     GRSEAGTMPQNPVYVRSIVTTALADEVAKTYGVTPIQVLTGFKYIGETILRLEQKGEQ
                     NRFVFGFEESYGYLAGSYVRDKDAVVASMLICEMAAYYTANGSSLKAELDRIYKTYGT
                     YLHKVDSFEFEGLAGMDKMKQLMADLRAGAPTQIAGYRVVRVEDYARSVSTDTATGEE
                     TPIDLPKADVLTYHLDCGASVIVRPSGTEPKIKTYYSAKGESHEAAAALQ"
BASE COUNT          229 a          369 c          371 g          183 t
ORIGIN      
        1 caaaagaccg acgtgttcaa ggatgtgcgc catgtcgagt ttgacaaggg catgcaggag
       61 ggcatcatcg agtatatctc cagcgaggtc atcgagcggt attacgacca tgtcaaggac
      121 cgcagcgtgc gccccagcgt ctgccgcggc tccggcttaa aactggtgta caccccgctc
      181 aacggcaccg gcaacctgcc cgtgcgccgc atcctggcgg acatcggcat cgaggacgtc
      241 accgtcgtgc ccgagcagga gctgccggac ggcaacttca ccacctgccc ctaccccaac
      301 cccgagatca agcaggccct gcagctgggg cttgacctgg cgaaaaagac cggcgcggac
      361 ctgctgctgg caaccgaccc cgacgccgac cgggtgggca ttgcggtgcg ggacggcggc
      421 gactacctgt tgctctccgg caacgaggtg ggcgtgctgc tgctggacta catctgcaag
      481 gggcgaagcg aggccggcac catgccccaa aacccggtct atgtgcgcag catcgtgacc
      541 accgccctgg cggacgaggt ggcgaagacc tacggcgtca cccccatcca ggtgctcacc
      601 ggctttaagt atatcggcga gaccatcctg cgcctggagc agaaaggcga gcagaaccgc
      661 tttgtctttg gctttgaaga gagctacggt tatctggcgg gcagctatgt gcgggacaag
      721 gacgcggtgg tcgccagcat gctgatctgc gagatggcgg cctattacac cgcgaacggc
      781 tcctcgctca aggcggagct ggaccgcatc tacaagacct acggcaccta tctgcacaag
      841 gtggactctt tcgagtttga gggcctggcc ggcatggata agatgaagca gctgatggcg
      901 gacctgcgcg cgggcgcgcc cacccagatc gcgggttacc gggtcgtgcg ggtggaagat
      961 tatgcccgca gcgtgtccac cgacactgcc accggcgagg agacccccat cgacctgccc
     1021 aaggcggatg tgctgaccta tcacctggac tgcggcgcca gcgtcatcgt gcgcccgtcc
     1081 ggcaccgagc cgaagatcaa gacttactat tcggccaagg gcgagagcca cgaggcggcg
     1141 gcggccctgc ag
//
LOCUS       DBMDYA010000804         1150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22363_length_1150_cov_1.146806, whole genome shotgun sequence.
ACCESSION   DBMDYA010000804 DBMDYA010000000
VERSION     DBMDYA010000804.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22363_length_1150_cov_1.146806"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="ACZPFK_14345"
     CDS             complement(<1..243)
                     /locus_tag="ACZPFK_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1407537.1"
                     /translation="MQEIEQLFRLYQKEVYGYLLFLTHSPQAAEDLLSETFLQALQGL
                     RRFRGQSSAKTWLFGIARNLWLKSLRGKRETVEFDDL"
     gene            complement(581..>1150)
                     /locus_tag="ACZPFK_14350"
     CDS             complement(581..>1150)
                     /locus_tag="ACZPFK_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HIR1407538.1"
                     /translation="KQTAARMLKKLLADGQREIDYLETVLYEVGQAGGEAALAEIRAE
                     LKAGGYLKNDRRKEKKQKPADFLRYTSSDGFEILVGRNNAQNDRLTLHTARGRDLWFH
                     VKDAPGSHTVVLSGGREIPLATQNEAAMLAVFHSSQAASAKVPVDFTEVRNIRKTGDL
                     KPGMVLYEKYETAYVTPDPQVLKKLGAGG"
BASE COUNT          178 a          399 c          347 g          226 t
ORIGIN      
        1 cagatcgtcg aattccacag tctccctttt gccccgcagg gatttgagcc aaaggttgcg
       61 ggcaatgccg aacagccagg tcttggccga ggattggccg cgaaagcggc gcaggccctg
      121 cagtgcctgc agaaaggtct cggagagaag atcctccgcc gcctgcgggg agtgggtgag
      181 gaagagcaga tacccgtaca cctctttttg atagagccgg aagagctgtt cgatctcctg
      241 cacgccgctt ccctcctctc cgtgtccttt cacctcatga gttccacttt tcccgctttt
      301 attacagccg cgcccgcaaa aagtcaaaaa ttttgccgcc cgcggcgggc cgaccggtcg
      361 cgccggggct tggcggcgta ttcggccctc agggaggcgc gccccgcccc catacagctg
      421 cgcgtcttgg cgccgcccgc cccattgagc tgcgcctttt gcgggtgcgc cgtctcggcg
      481 cccggccgca gccaaaaaga aagcgctccc acccgccaag gggatgggag cgcatgcgcg
      541 ccttttcaag actggagccg cggcaaacgc cgccgggccg tcacccgccc gcccccagtt
      601 ttttcagcac ctggggatcg ggggtgacat aggcggtctc gtatttctcg tacagcacca
      661 tccccggctt caggtcgccg gtcttgcgga tgttgcgcac ctcggtaaaa tccaccggca
      721 ccttggccga ggccgcctgg ctggagtgga agaccgccag catcgccgcc tcattttggg
      781 tggcgagcgg gatctcccgg ccgcccgaga gcaccaccgt gtggctgccc ggcgcatcct
      841 tgacgtggaa ccacaggtcc cgcccccggg ccgtgtgcag ggtgaggcgg tcgttctggg
      901 cgttgttgcg ccccaccaat atctcgaacc cgtccgagga ggtgtagcgc aaaaaatccg
      961 caggcttttg ctttttttcc ttgcggcggt cgtttttgag atagcccccc gctttcagct
     1021 cggcgcggat ctctgccagc gccgcctcgc cgcccgcctg ccccacctcg tagagcacgg
     1081 tctccagata gtcgatctcc cgctggccgt ccgccagcag ctttttcagc atccgcgcgg
     1141 cggtctgctt
//
LOCUS       DBMDYA010000805         1147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22450_length_1147_cov_0.838432, whole genome shotgun sequence.
ACCESSION   DBMDYA010000805 DBMDYA010000000
VERSION     DBMDYA010000805.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22450_length_1147_cov_0.838432"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1147
                     /locus_tag="ACZPFK_14355"
     CDS             <1..>1147
                     /locus_tag="ACZPFK_14355"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1407539.1"
                     /translation="GVAAKLPPLRSFYDGLLGFFGETRNGLFFAPLFLLLGGWLAGRR
                     WPGRRAAAAGLALALGCMTAEALALHQLGLQRRDTMYLLLPPVMVFLFSLLARRRGAG
                     RPGLRRLSTLVYLLHPLVIVAVRGLARPLGLWWLLVDCSPVHYLAVAVGSLLLAAALL
                     FVRDKLPPPPGGEKRDRPGFCKGEELPPHVAPVPPVQEARRLWAEIDLSAMRENAAAL
                     QALLPEGCRLMAVVKADAYGHGDLEVCRALAPAGVDAFAVATADEAVRLRRQGVEGEL
                     LVLGATPACRAGELAALDIAQTAADAAHARALSAAAAEVGTTLRVHLAVDTGMHRLGV
                     DAADPAAVEELLRLPHLRAAGIYTHLCVPESEEPEDLAFTKRQIARFY"
BASE COUNT          119 a          413 c          431 g          184 t
ORIGIN      
        1 gggtgtcgcc gccaagctgc ccccgctgcg gtcgttctac gacggcctgc tgggcttttt
       61 tggcgagacc cgcaacggcc tgtttttcgc gccgctcttt ttgctgctgg gcggctggct
      121 ggccgggcga cgctggcccg gcaggcgggc ggctgccgcc gggctggcgc tggcgctggg
      181 ctgcatgacc gccgaggcgc tggcgctgca ccagctgggg ctgcagcgca gggacaccat
      241 gtacctgctg ctgccgcccg tcatggtctt tttgttcagc ctgctggccc gtcgccgcgg
      301 cgcggggcgg cccggcctgc gccgcctctc cacgctggta tatctgctgc acccgctggt
      361 catcgtggca gtgcgcggcc ttgcccgccc gctggggctc tggtggctgc tggtggactg
      421 cagccccgtc cactacctgg cggtggcggt gggcagcctg ctgctggcgg cggccctgct
      481 ctttgtgcgg gacaagctgc ccccgccgcc gggcggcgaa aagcgggatc gccccggctt
      541 ttgcaaaggc gaagagctgc ccccgcatgt cgcccccgtt cccccggtgc aggaggcccg
      601 gcggctctgg gctgagatcg acctgtccgc catgcgcgaa aatgccgccg ccctgcaggc
      661 gctgctgccc gagggctgcc gcctgatggc ggtggtcaag gcggacgcct acggccacgg
      721 cgacctagag gtctgccgcg ccctggcccc tgccggggtg gacgcctttg ccgtggccac
      781 cgccgacgag gcggtgcggc tgcggcggca gggggtggag ggggagctgc tggtgctggg
      841 cgccaccccc gcctgccgcg ccggggagtt ggcggccctg gacatcgccc agacggccgc
      901 ggacgccgcc cacgcccggg cgctcagcgc ggctgcggcc gaggtcggga cgacgctgcg
      961 ggtccacctg gcggtggaca ccggcatgca ccggctgggc gtggatgccg ccgacccggc
     1021 cgcggtggaa gagctgctgc gcctgccgca tctgcgggcg gcgggcatct acacccatct
     1081 ctgtgttccc gaaagcgagg aaccggagga cctggccttt accaaacgcc agatcgcccg
     1141 cttttac
//
LOCUS       DBMDYA010000806         1142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22587_length_1142_cov_1.006724, whole genome shotgun sequence.
ACCESSION   DBMDYA010000806 DBMDYA010000000
VERSION     DBMDYA010000806.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22587_length_1142_cov_1.006724"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="ACZPFK_14360"
     CDS             <1..265
                     /locus_tag="ACZPFK_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR1407540.1"
                     /translation="GTGRSDYPNQVNNILAFPGIFKGALAVRAPRITDSMKEAAARGI
                     AAAVPPEKLCADYILPTAFDRGVADIVAKAVADEAVRLGINRV"
     gene            298..822
                     /locus_tag="ACZPFK_14365"
     CDS             298..822
                     /locus_tag="ACZPFK_14365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1407541.1"
                     /translation="MFEIRAAAREEYPLALQFYDDLTDAMEGAPYPPGWKKRVYPSED
                     FLKSSIACGELHLGWLDGSPAAAMVVNRAAAEGYEKAPWRVEALPGQVAVLHALGVLP
                     CHQGRGLARRMVEYAANFCRARGDRALRLDVLASNLPAVRLYPGLGFWRVGTARLFYE
                     DTGLTDFYLYEKAL"
     gene            937..>1142
                     /locus_tag="ACZPFK_14370"
     CDS             937..>1142
                     /locus_tag="ACZPFK_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1407542.1"
                     /translation="MEWKDIKITVPKAEAETAEAIATGIAGGGIYIEDYSDLEEQVEA
                     IAHVDLIEQELRDKDRSVVAVHLYL"
BASE COUNT          190 a          329 c          438 g          185 t
ORIGIN      
        1 gggcactggc cgcagcgatt atcccaacca ggtcaacaat attctggcct ttcccggcat
       61 cttcaagggg gcgctggcgg tgcgcgcgcc ccgcatcacc gacagcatga aagaggccgc
      121 cgcccgcggc atcgccgcgg ctgtgccgcc ggaaaagctc tgcgccgact atatcctgcc
      181 caccgccttt gaccggggcg tggcggatat cgtggcgaaa gccgtggccg acgaggcggt
      241 gcggctgggc atcaaccggg tttgattgag gccgggccgg cgagagaggg gaacgcgatg
      301 tttgagatcc gggcggcggc gcgggaggag tacccgctgg cgctgcaatt ctacgatgat
      361 ctgaccgacg ccatggaggg cgcgccctat ccgccggggt ggaaaaagcg ggtctatccg
      421 tcggaggact ttttgaagag ctccattgcc tgcggcgagc tgcacctggg ctggctggac
      481 ggcagccccg cggcggcgat ggtggtgaac cgggccgcgg ccgaggggta tgaaaaggct
      541 ccctggcggg tggaggccct gccggggcag gtggcggtgc tgcatgcttt gggggtgctg
      601 ccctgccatc agggcagggg gctggcccgc cggatggtgg agtatgccgc gaatttttgc
      661 cgggcgaggg gggacagggc gctgcggctg gatgtgctgg cctccaacct gcccgccgtc
      721 cgcctttatc ccggcctggg gttctggcgt gtggggacgg cgcggctgtt ttatgaggac
      781 accgggctca cggattttta tctgtacgaa aaagcgctgt gagcgcgcgg gggcggccgt
      841 ccccgccgac ctgcggaggc ccgcccggca gggcgggttt ttttggcgcc cctgggcggg
      901 cggcgcaaag acacgaaaaa aacggaggaa gacgccatgg agtggaagga tatcaagatc
      961 acggtgccca aagcggaggc ggagaccgcg gaggcgattg ccaccggcat cgctggcggc
     1021 ggcatctaca tcgaggacta ttcggacctg gaagagcagg tggaggccat cgcccatgtg
     1081 gacctgatcg agcaggagct gcgggacaag gaccgcagcg tggtggcggt gcacctttac
     1141 ct
//
LOCUS       DBMDYA010000807         1137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22692_length_1137_cov_1.437259, whole genome shotgun sequence.
ACCESSION   DBMDYA010000807 DBMDYA010000000
VERSION     DBMDYA010000807.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22692_length_1137_cov_1.437259"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="ACZPFK_14375"
     CDS             <1..517
                     /locus_tag="ACZPFK_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1407543.1"
                     /translation="KINASCDVIDASGSALPGLFAAGEVTGGVHGANRLGGNAVTDIV
                     VFGRIAGSSAERYVLANGGNTALTIKTEEEEAVVPEVEGGYKDGVYTGSGTGNGGPLT
                     VTVTVEGGNVVSVKLAEHAETDGIWQNAEKGVGNEIIRSQKTEVDVVAGATNTSNGIL
                     EAVKDALKDAK"
BASE COUNT          230 a          361 c          374 g          172 t
ORIGIN      
        1 caagatcaac gcctcctgcg atgtgatcga cgcctcaggc agcgcgctgc ccggcctgtt
       61 cgccgccggc gaggtcaccg gcggcgtgca cggcgccaac cgtctggggg gcaacgcggt
      121 caccgatatt gtggtcttcg gccgcatcgc cggctcttcc gccgagcgct atgtgctggc
      181 aaacggcggc aacaccgcgc tgaccatcaa gacggaagaa gaggaggcgg tcgtgcccga
      241 ggtggagggc ggctacaagg acggcgtcta caccggcagc ggcaccggta acggcggccc
      301 ccttaccgtg acggtcacgg tggagggagg caacgttgtc tcggtgaagc tggcggagca
      361 cgccgagacc gacggcatct ggcagaatgc tgaaaagggt gtgggcaacg agattatccg
      421 cagccagaaa accgaggtgg acgtggtcgc gggcgcgacc aacacctcaa acggcatcct
      481 cgaggccgtg aaagacgcgc tgaaagacgc aaaataagcg caaagccatc gcacacaaag
      541 cacggcccgg gagcttgatg attcccgggc cctgtttttt atcggcctgc ctatgagaat
      601 gcgtttgttg cggtgcaggc agagggtttt ctttccgccg ggcccacggc ggccgcgcga
      661 gacggcggaa ccgttgccgt agcgcgcccc ccgaccatcg agccgccgca aagcggtcgg
      721 agggcttttc tctcggggag aggtcttctt cccctctcgc tccgaggcgg ccaaaacagg
      781 gatgcgtcaa atgtggcgcc gccgtccctt tccgcccatg tatttcttca gggacgcggc
      841 ctcccccctc aaaaaagggc cgcggccgcc ctatggcccg gcccgggctg gaaaatgctg
      901 cggccttgtg ctggcgggcc ggttatggta gaataaaaca gatatcggcc cgccgcgtgc
      961 gcgctgaaaa aaatttgagg cttccctcac aaaggggcgt aacatcttga acgaatttga
     1021 acgccgccgc acccgcggcg cccacaaacc ctcaaagccg tcctcccggc aggatcggga
     1081 cggcagcccg gccgggcaga actggccgcc gcgccgagag ccgaaaaaaa agagagg
//
LOCUS       DBMDYA010000808         1136 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22708_length_1136_cov_1.631884, whole genome shotgun sequence.
ACCESSION   DBMDYA010000808 DBMDYA010000000
VERSION     DBMDYA010000808.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22708_length_1136_cov_1.631884"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="ACZPFK_14380"
     CDS             <1..281
                     /locus_tag="ACZPFK_14380"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1407544.1"
                     /translation="NLYCAVTRKNLNGEPEGGFYANECVDIYDAVDAYTVESAYTSFE
                     EDVKGRIKPGYYADLAVLSEDIFTLPADELRRTKVDATMMGGRFVYQR"
     gene            839..>1136
                     /locus_tag="ACZPFK_14385"
     CDS             839..>1136
                     /locus_tag="ACZPFK_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR1407545.1"
                     /translation="MEQKRFTVPGPVLDAQGRPVPGYSTRSVLHYDRSAIKASPFRIK
                     EWDFYQVSDREKCLQFTLGHASYAGQVGIMFFDFIKGEWIANFDKLLAFPFGSLH"
BASE COUNT          229 a          318 c          385 g          204 t
ORIGIN      
        1 acaacctcta ctgcgccgtg acccgcaaga acctgaacgg cgagccggag ggcggcttct
       61 atgccaacga gtgtgtggac atctacgacg cggtggacgc ctataccgtc gagagcgcct
      121 ataccagctt tgaggaggac gtgaaaggcc gcatcaagcc gggctattac gccgacctgg
      181 cggtgctcag cgaggacatc ttcaccctgc ccgccgacga gctgcgccgc accaaggtgg
      241 acgccaccat gatgggcggc cgcttcgtct accagagata atttgggctt ggatttatac
      301 agaacgttta gccgcgcggg ggttcccgcg cggctttttg ataaaaattt ttggtgaaaa
      361 aggggaatgc ctgcggaggg gggacgggag ccgctttccc ggcagggcgg cgggagaaga
      421 ggggccggga gagtagggag tggggcgcac ggggcgcgga ccctgtggag gtctcaaaaa
      481 tataagagat ttttttgttt tggggcggcg cttgcaccgg tcccccagcc cccgcggcgg
      541 ttccgccaaa aggggcgcca tgcggcgctg cctttggcgc tcagaaaagc cggaaaaggg
      601 gcagaagaaa agggaaaggc gggcgcggcc gctgtgacag gctgcaagag gctgcaacaa
      661 agcgcccctt gccccacggc ggaaaagcag actttggggc cgggcggttt tgaggctttg
      721 cggcctcccg cggccacggg gtcgtcacgg ccatagaaat aagcgggttt ttcttgtgca
      781 ctctgacggt tgcgggggac tgtgcggcaa cggtacaatg gacgtgaggt gaggtactat
      841 ggaacaaaag cggtttaccg tccccggccc ggtgctggac gcccagggcc gccctgtgcc
      901 cggctattcg acccgcagcg tgctgcacta cgaccgcagc gccatcaagg ccagcccttt
      961 ccgcatcaag gagtgggatt tttatcaggt cagcgaccgg gaaaagtgcc tgcagttcac
     1021 actgggccac gccagctatg cgggccaggt gggcatcatg ttcttcgact tcatcaaggg
     1081 cgagtggatc gccaacttcg acaagctatt ggctttcccg ttcggcagcc tgcaca
//
LOCUS       DBMDYA010000809         1127 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22948_length_1127_cov_0.905458, whole genome shotgun sequence.
ACCESSION   DBMDYA010000809 DBMDYA010000000
VERSION     DBMDYA010000809.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22948_length_1127_cov_0.905458"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="ruvA"
                     /locus_tag="ACZPFK_14390"
     CDS             complement(<1..485)
                     /gene="ruvA"
                     /locus_tag="ACZPFK_14390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1407546.1"
                     /translation="MIYSLTGKIMEKGLDRVVIECGGVGYEVGVPASAASALPPQGGA
                     TTLYTYLNITENDVSLFGFLTAADRQMFKLLTSVSGVGPKAGLAILSALPCDRIALAV
                     SAGDSKAFTAANGVGPKLGQRIVLELKDKVGKGLADGISLADVSGASSAPAGSASQAI
                     AA"
     gene            complement(581..1123)
                     /gene="ruvC"
                     /locus_tag="ACZPFK_14395"
     CDS             complement(581..1123)
                     /gene="ruvC"
                     /locus_tag="ACZPFK_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR1407547.1"
                     /translation="MRILGIDPGYAIVGYGALDYNAGRFGVVEFGAITTQAHTLFEER
                     LVSVYDEMAALLERTRPEALAIETLFYAHNQSTVIYVAEARGVILLAARKAGVPVFEY
                     TPLQVKQAVAGYGKAVKKQVQEMTRAILKLDKVPKPDDTADALAMAICHAHSSRSQLF
                     GRTGTVNNYNLRPGDLSRRR"
BASE COUNT          172 a          387 c          374 g          194 t
ORIGIN      
        1 gcggcgatgg cctgggaggc gctgcccgcg ggcgccgacg acgccccgga gacgtcggcc
       61 agcgagatgc cgtccgccag ccccttgccc accttgtcct tgagctccag cacgatgcgc
      121 tggcccagct tcggccccac gccgttggcg gcggtaaacg cttttgagtc ccccgccgac
      181 acggccagag cgatgcggtc acagggcagc gccgacagga tggcgagccc ggctttgggc
      241 cccacgccgg agacgctggt gagcagcttg aacatctgcc ggtccgccgc cgtgagaaag
      301 ccgaacagcg agacgtcgtt ttccgtgata tttaaatagg tataaagggt ggtggcgccg
      361 ccctgggggg gcagcgccga ggccgcggag gcgggtaccc ccacctcata gcccacgccg
      421 ccgcactcga tgacgacccg gtccaggccc ttttccatga ttttgccggt cagggaatag
      481 atcatatctc tcctcttgcc tcccgccgtc gcgctgacgg catctgtctt gatcggcccg
      541 cgccgcgggc gaatcggtca cgggcggggc cgcgggcccc tcagcgcctg cggctcaggt
      601 cgccggggcg caggttgtaa ttgttcaccg tgccggtgcg gccaaagagc tggctgcggc
      661 tggagtgggc atggcagatc gccatggcca gcgcgtcggc cgtgtcgtcg ggctttggca
      721 ccttgtccag cttcaggatg gcgcgggtca tctcctgcac ctgctttttg accgccttgc
      781 catacccggc caccgcctgt ttgacctgca gcggcgtgta ttcgaacacc ggcacccccg
      841 ccttacgggc ggccagcagg atgaccccgc gcgcctcggc cacatagatg acggtgcttt
      901 ggttgtgggc gtaaaacagc gtctcgatgg ccagcgcctc gggcctggtg cgctccagca
      961 gagccgccat ctcatcgtaa acgctgacca gccgctcttc aaacagggtg tgggcctggg
     1021 tggtgatggc cccgaactcc accaccccaa agcgtccggc gttatagtcc agcgcgccgt
     1081 agccgacgat ggcgtagccc gggtcgatgc cgagaatgcg cacgcgc
//
LOCUS       DBMDYA010000810         1126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22960_length_1126_cov_1.574634, whole genome shotgun sequence.
ACCESSION   DBMDYA010000810 DBMDYA010000000
VERSION     DBMDYA010000810.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22960_length_1126_cov_1.574634"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="ACZPFK_14400"
     CDS             complement(<1..452)
                     /locus_tag="ACZPFK_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407548.1"
                     /translation="MSKKDNAALTPGEKYINWVHNFGRVGIIVIILYMLCMPVITCAV
                     FNCFPPIASVLKGGAGVLALFIPLGISEVLSYTPIVGSACYVTFLTGNVMNLKLPCAI
                     NAMKLAKVDQSTEKGDAISTVAVAASSILTICVIAVGVLLAVPLNKSG"
     gene            complement(471..>1126)
                     /locus_tag="ACZPFK_14405"
     CDS             complement(471..>1126)
                     /locus_tag="ACZPFK_14405"
                     /inference="COORDINATES: protein motif:HMM:NF027796.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR1407549.1"
                     /translation="AAYAAVFLKKAYGRCLELGFDKKTVNGVIKSSLTFSVVPSISIV
                     IGFLTLSAALGVPWPWWRLSVLGAVGYELMAADMATKGMGYESLAAMAQANDPKVFGA
                     VMFVMTIGIIGGLIFVLVPFGKKLTTGMMKARKSTSTWGVVFSSCFMLTMVAVFLPLM
                     VTGDPVQVAVLVTSAVVTVLLGLLIKVTKWGWLNDFVLAITLIVSMVSAVGWNALLH"
BASE COUNT          233 a          348 c          347 g          198 t
ORIGIN      
        1 atgccgctct tgttgagagg cactgccaac agaacgccaa ccgcgatgac gcagatggtc
       61 aggatgctgc tggccgcgac cgcgacggtg gagatggcgt cgcccttttc agtgctctgg
      121 tccacctttg ccagcttcat ggcgttgatg gcgcagggca gcttcaggtt catgacgttg
      181 ccggtcagaa aggtgacgta gcaggccgag ccgacgatgg gggtgtagct gagcacctcg
      241 gagatgccca gcgggatgaa gagcgccagc acgccggcgc cgcccttgag cacgctggcg
      301 atgggcggga agcaattgaa caccgcgcag gtgatgaccg gcatgcagag catgtacagg
      361 atgatgacga tgatgcccac acggccgaaa ttgtggaccc agttgatgta tttttcgccc
      421 ggggtcaggg cggcgttatc ttttttactc aacttttttc cctccctttc tcagtgcagc
      481 agcgcgttcc agccgacggc cgaaaccatg gagacgatca gcgtgatggc caggacaaaa
      541 tcgttgagcc agccccattt cgtcaccttg atcagcagcc ccagcagcac cgtgaccacc
      601 gcgctggtca ccagcaccgc cacctgcacc gggtcgccgg tgaccatcag cggcaggaag
      661 accgcgacca tggtcagcat gaagcagctg ctgaagacga cgccccaggt gctggtgctc
      721 ttgcgggctt tcatcatgcc ggtggtcagc tttttgccga aaggcaccag cacgaagatc
      781 aggccgccga tgataccgat ggtcatgaca aacatcaccg cgccgaacac cttggggtcg
      841 ttggcctgcg ccatggcggc caagctctcg tagcccatgc ccttggtggc catgtccgcc
      901 gccatcagct catagcccac agcgcccagc accgacaggc gccaccaggg ccagggcacg
      961 cccagcgccg cggagagggt cagaaagccg atgacgatgg agatcgaggg caccaccgaa
     1021 aaggtcaggc tggacttgat gacgccgttg accgttttct tgtcgaagcc cagctccagg
     1081 cagcggccgt aagctttctt cagaaagacg gccgcatagg ccgcca
//
LOCUS       DBMDYA010000811         1126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_22968_length_1126_cov_1.169756, whole genome shotgun sequence.
ACCESSION   DBMDYA010000811 DBMDYA010000000
VERSION     DBMDYA010000811.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22968_length_1126_cov_1.169756"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="purF"
                     /locus_tag="ACZPFK_14410"
     CDS             <1..1032
                     /gene="purF"
                     /locus_tag="ACZPFK_14410"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1407550.1"
                     /translation="FHTTSDTEVIAYAITKERIHAPSIEAAVEAAMGQLAGAYSLVIS
                     SPSKLIAVRDPVGFRPLCMGRMGESILFASESCAFDAVGAEFVRDIEPGEIVVVDAQG
                     VRSIRSRCGGPSAMCVFEFIYFARPDSVIDGSSVHIARQRAGAFLALQHPVQADVVIG
                     VPDSGLDAALGYAKQSGIPYDIGFLKNKYIGRTFIAPTQKQREDDVRIKLNPIASVVR
                     GKRVVLVDDSIVRGTTSLKIVKLLREAGAKEVHMRLSAPPFLYPCYFGTDIDSSEHLI
                     AYQHTVEEIRSIIGVDSLGYLDVGCLDKLADNSKCGFCNGCFTGKYPVEPPKKSQKSK
                     FETKISAQT"
BASE COUNT          216 a          355 c          367 g          188 t
ORIGIN      
        1 ttccacacca ccagcgacac cgaggtcatc gcctacgcca tcaccaaaga gcgcatccac
       61 gcccccagca tcgaggcggc ggtggaggcg gccatgggcc agctggcggg ggcctacagc
      121 ctggtcatct cctcgcccag caaattgatc gccgtgcgcg acccggtggg cttccgtccg
      181 ctgtgtatgg gccgcatggg cgagagcatc ctctttgcca gcgagtcctg cgcgttcgac
      241 gccgtgggcg ccgaatttgt gcgggacatc gagccgggcg agatcgtggt ggtggacgcg
      301 cagggggtgc gcagcatccg cagccgctgc ggcggaccct cggcgatgtg cgtctttgag
      361 ttcatctatt ttgcccgtcc ggattcggtc atcgacggct ccagcgtgca catcgcgcgg
      421 cagagggcgg gcgcttttct ggcgctgcag cacccggtac aggccgacgt ggtcatcggg
      481 gtacccgatt ccggcctgga cgccgcgctg ggttacgcca agcagagcgg catcccctac
      541 gacatcggct ttttgaaaaa caaatacatc ggccgcacct ttatcgcccc cacccagaag
      601 cagcgggagg acgacgtgcg catcaagctc aaccccatcg cctcggtggt gcggggcaag
      661 cgggtggtgc tggtggacga ctccatcgtg cgcggcacca ccagcctgaa gatcgtcaag
      721 ctgctgcggg aggcgggggc aaaagaggtg cacatgcgcc tttcggcgcc gccgttcctc
      781 tacccctgct atttcggtac cgacatcgac tccagcgagc acctgatcgc ttatcagcac
      841 acggtggagg agatccgcag catcatcggg gtggacagcc tgggctacct ggacgtgggc
      901 tgcctggaca agctggccga caactccaaa tgcggcttct gcaacggctg ttttaccggg
      961 aaatacccgg tggagccgcc gaaaaagagc cagaaaagca agttcgagac aaagatttca
     1021 gcacagacat aaacggggcc gccgtcaggg cggcaggcgg cctgcgggcc gtccccggtg
     1081 acgaggagaa gttatggaaa aaagctttag cgagagctac cgcgcc
//
LOCUS       DBMDYA010000812         1122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23082_length_1122_cov_0.915769, whole genome shotgun sequence.
ACCESSION   DBMDYA010000812 DBMDYA010000000
VERSION     DBMDYA010000812.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23082_length_1122_cov_0.915769"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="ACZPFK_14415"
     CDS             <1..654
                     /locus_tag="ACZPFK_14415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1407551.1"
                     /translation="DIPLYLAHTITDIVGKHGRVEKVVVSAVDENRNPVPGSEMEFDC
                     DTVLLSVGLIPENELSRNAGIEIDRRTSGPVVFENMETSIPGVFAAGNVVHVHDLVDF
                     VTGESQRAGRAAADYIAGNSAGGGADLELVNGAHVNYTVPQKIRLAGVDKAAGVFFRV
                     NKVMAAGTVKVTSGDTQIAAFKRQHMAPGEMEQIQLPKALLEKAHGTVTISVEEANA"
     gene            654..1001
                     /locus_tag="ACZPFK_14420"
     CDS             654..1001
                     /locus_tag="ACZPFK_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1407552.1"
                     /translation="MAIRKMICITCPRGCHLEVDDETLQVRGNTCPKGEEYGRNEVTD
                     PRRVLTSTVRIEGAAHCRLPVKTAAPIPKPLLLKAMDALAEVTVKAPVHVGDVVIRDL
                     LGTGVDLVATKEM"
BASE COUNT          210 a          335 c          405 g          172 t
ORIGIN      
        1 gacatcccgc tgtatctggc gcacaccatc accgacatcg tgggcaagca cggccgggtg
       61 gaaaaggtgg tcgtcagcgc cgtggatgag aaccgcaacc cggtgccggg cagcgagatg
      121 gagttcgact gcgacacggt gctgctctcg gtgggcctga tcccggaaaa cgagctctcc
      181 cgcaacgcgg gcatcgagat cgaccggcgc accagcgggc cggtggtatt tgagaatatg
      241 gagacctcga tccccggcgt ttttgcggcg ggcaacgtgg tgcatgtgca tgacctggtg
      301 gactttgtca ccggcgagag ccagcgggcc ggccgtgcgg cggcggatta catcgcgggc
      361 aactcggccg gcggcggcgc ggacctggag ctggtgaacg gcgcgcatgt caactacacc
      421 gtgccgcaaa aaattcgcct ggccggcgtg gacaaagcgg ctggggtctt tttccgggtg
      481 aacaaggtga tggcggcggg caccgtcaag gtgaccagcg gcgacaccca gatcgcggcg
      541 ttcaagcgcc agcacatggc cccgggtgag atggagcaga tccagctgcc caaggcgctg
      601 ctggaaaagg cgcacggtac ggtcacgatt tctgtcgagg aggcgaatgc ataatggcga
      661 tccgcaagat gatctgcatc acctgccccc gcggctgcca cctggaggtg gacgacgaga
      721 cgctgcaggt gcggggcaat acctgcccca agggggagga gtacggccgc aacgaggtca
      781 ccgacccgcg gcgggtgctg acctccaccg tgcgcatcga gggcgcggcc cactgccgcc
      841 tgccggtcaa gacggcggcc cccatcccaa agcccctgct gctgaaagcg atggatgcgc
      901 tggccgaggt cacggtgaaa gcgccggtgc atgtggggga cgtggtgatc cgcgacctgc
      961 tgggcaccgg tgtggacctg gtggcgacga aagagatgta acctgcaggg atgcagcaaa
     1021 aaacgcgggg ggctttgcct cccgcgtttt tttgtcccgg cctcccggcc gcaccgcaga
     1081 gcgggagcgg gcacagggat agattgtttt tgccgcgagg cg
//
LOCUS       DBMDYA010000813         1115 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23238_length_1115_cov_1.886588, whole genome shotgun sequence.
ACCESSION   DBMDYA010000813 DBMDYA010000000
VERSION     DBMDYA010000813.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23238_length_1115_cov_1.886588"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="ACZPFK_14425"
     CDS             complement(<1..1094)
                     /locus_tag="ACZPFK_14425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1407553.1"
                     /translation="MKRLSDLLLCLALLLAGCGQTDRPSSAAVSAPPATAQPSPAPTP
                     QPAVDFSATGDNLIHGQLYLQAQRRTEDGSYDFMPLYEKVASFYAGRDLNFINQETLV
                     NDELPPSSYPLFSSPAAVGRTAYDLGFRLFGTSNNHSYDLGAAGIAATRRFWASMPDD
                     ALAFGLWENGAEDDIPLYEKNGVTFACLAYTQYTNGLPTPQGAEAHVILTSDTETIER
                     QITAARAMADVVLVSVHWGTEDSHTVTEGQRALARQMAGWGADLIIGTHPHVLQGAEW
                     LEREDGGRSFVAYSLGNFVSAQSRPDQLIGAVLSCTFTKTGEGAAEISDVRFHPTVTH
                     YGSRYSDVKVYFLSDYSADLALGHGVRTDYP"
BASE COUNT          197 a          340 c          386 g          192 t
ORIGIN      
        1 ggatagtcgg tgcgcacccc gtgccccagg gcgaggtcgg cgctgtaatc gctgagaaaa
       61 tagaccttga cgtcgctgta gcggctgccg taatgggtga cggtgggatg aaagcgcacg
      121 tcgctgatct ccgccgcgcc ctccccggtc ttggtaaaag tacagctcag caccgcgccg
      181 atgagctggt ccggcctgct ctgcgctgag acaaagttgc ccagggaata ggccacaaag
      241 ctgcggccgc cgtcctcccg ctccagccac tcggccccct gcagcacatg ggggtgggtg
      301 ccgatgatca gatcggcgcc ccagcccgcc atctgccggg caagcgcgcg ctgcccctcg
      361 gtgacggtgt ggctgtcctc ggtgccccag tgcaccgaga ccagcacgac gtccgccatc
      421 gcccgcgccg ccgtgatctg gcgctcgatg gtctcggtgt ccgaggtgag gatcacatgg
      481 gcctccgctc cctgcggggt gggcaggccg ttggtgtatt gcgtgtaggc gagacaggca
      541 aaggtgacgc cgtttttctc atagagaggg atgtcgtcct cggcgccgtt ctcccaaagc
      601 ccgaacgcca gcgcatcgtc cggcatggag gcccaaaagc gccgggtggc ggcaatgccc
      661 gcggcgccca gatcatagct gtggttgttg gaggtgccga acagccggaa ccccaggtca
      721 taggcggtgc ggcccaccgc ggcgggggag gagaacagcg gatagctgct cgggggcagc
      781 tcgtcgttca ccagggtctc ctgattgata aagttgaggt cccgcccggc ataaaaagag
      841 gcgacctttt catagagcgg cataaaatca tagctgccgt cttccgtcct gcgctgtgcc
      901 tgcaggtaga gctggccgtg gatcaggttg tcgccggtgg cggaaaaatc caccgcaggc
      961 tgcggcgtgg gggccggcga gggttgcgcc gtcgcaggcg gggcagagac cgccgcagaa
     1021 gaggggcggt cggtctggcc gcagccggcc agcagcaggg caaggcataa gagcaggtcg
     1081 gaaagtcgtt tcattttttg atcaaatcct tcata
//
LOCUS       DBMDYA010000814         1114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23268_length_1114_cov_1.520237, whole genome shotgun sequence.
ACCESSION   DBMDYA010000814 DBMDYA010000000
VERSION     DBMDYA010000814.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23268_length_1114_cov_1.520237"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="ACZPFK_14430"
     CDS             <1..220
                     /locus_tag="ACZPFK_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1407554.1"
                     /translation="EKTAVKRSLGEALREHRVRCKMTQEFVAEAVGVSRQAVSKWENG
                     ASDPSTSNLCMLAKLYGVPPEELLRAAE"
     gene            complement(516..1100)
                     /locus_tag="ACZPFK_14435"
     CDS             complement(516..1100)
                     /locus_tag="ACZPFK_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR1407555.1"
                     /translation="MKKMILVTSPPGCGKTRLSKRLAAALHHVVYLDKDTLIPLSKRI
                     FAVAGQPYDRSSDFFEENIRNYEYDVILALGFEALSYDNTVLINAPFSREVRDAAYMQ
                     ALKEKLAAQGAKLVVVWIVTDPEVCHQRMIQRNSDRDTWKLTHWDEYIAGVDFSIPDT
                     LGDPEDNENLLLYHNSTDEMADASLARILPLLED"
BASE COUNT          202 a          327 c          376 g          209 t
ORIGIN      
        1 ggagaagacc gcggtgaaga ggtcgctggg agaggccctg cgggaacacc gggtgcgctg
       61 taaaatgacc caggagtttg tggccgaggc ggtgggagtg agcaggcagg cggtgtcgaa
      121 atgggagaac ggcgcctccg accccagcac ctccaacctg tgcatgctgg caaagctgta
      181 cggcgtcccg ccggaagaac tgctgcgggc ggcggagtga gggctgcaaa ggggcgaaaa
      241 cagcggcccg ggcggtgcgg ggccctgtcc ccgtctttcg tccgtcgctg aaaaaagccg
      301 ggggcgcccc ccggcggttt cacccaggga gagcacaggc agagaggacc ggccttgaaa
      361 aaggccggtc ctctctgttc atctgtttgc gcttattttg tgccgatcaa ggggcaaaag
      421 ccgccgctgc ttctcccggc gcgggcatcg cccccgcaga cgcatcggcc cggcttttgc
      481 cggatcaaaa tccgggctgg ccgcccgggt gcttttcagt cctccagcaa gggcagaatc
      541 cgcgccagcg aggcgtccgc catctcgtcg gtggagttgt ggtagagcag caggttctcg
      601 ttgtcctccg ggtcgcccag cgtgtcgggg atgctgaaat ccaccccggc gatatactcg
      661 tcccaatggg tgagcttcca ggtgtcccgg tcggaattgc gctggatcat ccgctggtgg
      721 cagacctcgg ggtcggtgac gatccagacc accaccagct ttgcgccctg tgccgcgagc
      781 ttttccttga gggcctgcat gtaggcggcg tcccgcacct cgcggctgaa cggcgcgttg
      841 atgaggacgg tgttgtcata gctgagcgcc tcaaacccca gcgccaggat gacgtcgtat
      901 tcataattgc ggatattctc ttcaaaaaaa tccgagctgc ggtcgtaggg ctgtccggct
      961 accgcgaaaa tgcgtttgga gaggggaatc agcgtgtcct tgtccagata gaccacgtgg
     1021 tgcagggccg ccgccagccg cttggaaaga cgggtcttgc cgcatccggg cggagaggtg
     1081 accaggatca tttttttcat tcagagaacc tcct
//
LOCUS       DBMDYA010000815         1108 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23429_length_1108_cov_1.577954, whole genome shotgun sequence.
ACCESSION   DBMDYA010000815 DBMDYA010000000
VERSION     DBMDYA010000815.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23429_length_1108_cov_1.577954"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            249..>1108
                     /locus_tag="ACZPFK_14440"
     CDS             249..>1108
                     /locus_tag="ACZPFK_14440"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR1407556.1"
                     /translation="MKRALRAAASLLLVFALAVMVFPSQGVHAAADVVNIYHTNDVHG
                     GVEASFNEETKEIEKPGLDYVAALHAADENSLLVDAGDFSQGTLFANMAHGLSVVEVM
                     NAAGYDLGVLGNHEFDYSVADLEANVKAAEFDIISANVTIDDAHASENPTLAAMPRYV
                     VKTAGGHKIAFFSCDTPELNGMVSPATLKAGGVQVRTDISALAAEIVAEIGEKEPDVE
                     AVIAVTHCGLSTGTANETSNDIAKVEGVTAVIDGHDHQLRLGDSAVDVNGTLIVSTGS
                     NLENVGCLTL"
BASE COUNT          218 a          333 c          386 g          171 t
ORIGIN      
        1 tttccccctg gtggtggcgg gcggtgtgac ggcagccacg ctggccgaaa agctgactgt
       61 ggcggacgcg gtcattgcgg accgcgcttt tcgcgataac ggccgggaca ccggcgaggt
      121 ggagcccggc agaataaaag cctttttaca ggaggccgcg cggctcaggg accggtgagg
      181 gctgcggaac acaaaggggg ttctgctgcg ccgcctgcgg cgcagaaaag ataaaaggag
      241 gaaaagaaat gaaaagagca ctgagggcgg cagcttcgct gttgctggtc ttcgcgctgg
      301 ccgtaatggt cttcccgtcc cagggggtcc atgcggcggc ggatgtggtg aacatctacc
      361 acaccaacga cgtgcacggc ggcgtcgagg cctcgttcaa cgaggagacc aaggagatcg
      421 aaaaacccgg cctggactat gtggcggcgc tgcacgcggc ggatgagaac tcgctgctgg
      481 tggacgcggg cgacttttcc caggggacgc tgttcgccaa catggcccac ggcctgagcg
      541 tggtggaggt gatgaacgcc gccggctacg atctgggcgt gctgggcaac cacgagttcg
      601 actattcggt ggcggacctg gaggccaacg tgaaagccgc ggagtttgac atcatctccg
      661 ccaatgtcac catcgacgac gcccacgcgt ctgaaaaccc caccctggcg gccatgccgc
      721 gctatgtggt caagacggcg ggcggccaca agatcgcctt tttcagctgc gacacgccgg
      781 agctcaacgg catggtgagc cccgccaccc tgaaagcggg cggcgtgcag gtgcgcaccg
      841 acatctcggc gctggcggct gagatcgtgg cggagatcgg ggagaaagag cccgacgtgg
      901 aggccgtcat cgcggtcacc cactgcggcc tgtccaccgg caccgccaac gagacctcca
      961 acgacatcgc aaaggtggag ggggtcaccg cggtcatcga cgggcatgac caccagctgc
     1021 ggctgggcga ttctgccgtg gacgtgaacg gcaccctgat cgtctccacc ggctccaacc
     1081 tggagaacgt gggctgcctg acgctgga
//
LOCUS       DBMDYA010000816         1106 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23486_length_1106_cov_1.516418, whole genome shotgun sequence.
ACCESSION   DBMDYA010000816 DBMDYA010000000
VERSION     DBMDYA010000816.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23486_length_1106_cov_1.516418"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /gene="aroC"
                     /locus_tag="ACZPFK_14445"
     CDS             <1..977
                     /gene="aroC"
                     /locus_tag="ACZPFK_14445"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1407557.1"
                     /translation="RRGEASLSTARREADEFELLSGFFEGHTTGAPLAILIRNGDAHS
                     KDYGQLKAKPRPSHADYTAFCKYHGFQDYRGGGHFSGRVTAPVVVAGAVCQQILASHG
                     VMLGTHIARCGGVADAGLPQDGRLLKTALERCNGSDFPVLDEAAGAAMKARIAAARAG
                     LDSVGGVLETAVLGLEAGLGEPWFWSVESELAHLLFSVPAVKGVEFGAGFAIAEQAGS
                     EANDPFYWDGDGAVRTRTNHSGGAQGGITNGMPLIVRTAVKPTPSIYRPQQTVDLEKR
                     EEAVLSIQGRHDPAIVHRARAVVDAVVALGLVDLAAARHGTDWMAEKF"
BASE COUNT          189 a          361 c          390 g          166 t
ORIGIN      
        1 agcggcgcgg ggaggcgtcg ctctccaccg cccgccgaga ggcggacgaa ttcgagctgc
       61 tcagcggctt ttttgagggg cacaccaccg gtgcgcctct ggccatcctg atccgcaacg
      121 gcgacgcgca ctcaaaggac tacggtcaac tgaaagcaaa gccccgcccc tctcacgccg
      181 attacacagc cttttgcaaa taccacgggt ttcaggacta tcgcggcggc ggccattttt
      241 ccggccgtgt caccgccccg gtggtggtcg ccggcgccgt ctgtcagcag atcctcgcca
      301 gccacggcgt gatgctgggc acccacatcg cccgctgcgg cggcgtggcc gacgcggggc
      361 tgccacagga cggccgcctg ctgaaaaccg cgctggagcg ctgcaacggc agcgatttcc
      421 cggtgctgga cgaggcggcc ggcgccgcca tgaaagcccg catcgccgcc gcccgggccg
      481 ggctggactc ggtgggcggg gtgctggaga ccgcggtgct ggggctggag gccgggctgg
      541 gcgagccctg gttctggtcg gtggagagcg agctggccca tctgctgttc agcgtgcccg
      601 ccgtcaaggg cgtcgagttc ggcgccggtt tcgcgatcgc cgagcaggcg ggcagcgagg
      661 ccaatgaccc cttttattgg gacggggacg gcgctgtgcg cacccgtacc aaccacagcg
      721 ggggcgcgca ggggggcatc accaacggca tgccgctgat cgtgcgcacc gccgtcaagc
      781 ccaccccttc catctaccgc ccgcagcaga cggtggatct ggaaaaaagg gaggaagcgg
      841 ttctgtccat tcagggccgc catgacccgg ccatcgtaca ccgggcgcgg gcggtggtgg
      901 atgccgtggt cgccctgggg ctggtggacc tggccgcggc gcggcacggc accgactgga
      961 tggccgaaaa gttttgatca cgcacggaag ccgtgcagac ggtaaaaaag ggagagaaca
     1021 ccatggaaac gcagcggtac ggcctggttg gggagcatct gggccacagc ttttcaaaat
     1081 tgatccacga gcagctcgca gactac
//
LOCUS       DBMDYA010000817         1096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23750_length_1096_cov_1.717588, whole genome shotgun sequence.
ACCESSION   DBMDYA010000817 DBMDYA010000000
VERSION     DBMDYA010000817.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23750_length_1096_cov_1.717588"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="ACZPFK_14450"
     CDS             complement(<1..296)
                     /locus_tag="ACZPFK_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1407558.1"
                     /translation="MGEKRIDQKLPPRRRIFDLTLRCLICFCAGLTALLAAALILYIL
                     ARGLPGVTWQLISSEESYLNDTIGILPSILNTLYIIVAALAIVLPLGVGAAVYLT"
     gene            complement(298..1095)
                     /gene="pstC"
                     /locus_tag="ACZPFK_14455"
     CDS             complement(298..1095)
                     /gene="pstC"
                     /locus_tag="ACZPFK_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1407559.1"
                     /translation="MLLITVYMVGAGLPALREIGLGNFLFGREWASTAQDPKFGILPF
                     ILTSVYGTAGATLIGVPVGLLTAVFLAKVAPKKLADRVRPAVDLLAGIPSVVYGLIGV
                     SVLVPLIARVFGLPKGTSLLAAMLVLSVMILPSIISVSETALRAVPPEYEEASLALGA
                     TPTETIFKVSIPAAKSGIAAGVVLGIGRAVGEAMAVMMVAGNVANMPQLFGSVTFLTT
                     AISKEMSYSSGLQRQALFSIALVLFLFIMVINIVLNVALKGGKKGGR"
BASE COUNT          216 a          350 c          366 g          164 t
ORIGIN      
        1 gtcagataga ccgccgcgcc cacccccagc ggcagaacga tggccagggc ggcgacgatg
       61 atgtacagcg tgttgaggat gctgggcagg atgccgatgg tgtcgttgag atagctctcc
      121 tccgacgaga tcagctgcca ggtgacgccg ggcaggccgc gagccaggat atagaggatc
      181 agcgccgccg ccagcagcgc cgtgaggccc gcgcaaaagc agatgaggca gcgcagcgtc
      241 aggtcaaaaa tgcggcgccg gggcggcagc ttttggtcaa tccgtttttc tcccatgtta
      301 tctgcccccc tttttgccgc ccttgagggc cacgttcagg acaatattga tcaccatgat
      361 aaagagaaag aggaccagcg cgatggagaa cagcgcctgg cgctgcagcc cggacgagta
      421 gctcatctcc tttgagatgg cggtggtgag aaaggtgacg ctgccgaaaa gctgcggcat
      481 gttggccaca ttgcccgcca ccatcatcac cgccatggcc tcgcccacgg cccggccgat
      541 gcccagcacc acccccgccg cgatgccgct tttggccgcc gggatgctga ccttgaagat
      601 ggtctcggtg ggcgttgccc ccagggccag gctggcctct tcatattccg gcggcaccgc
      661 ccgcagcgcg gtctcggaca cgctgatgat ggagggcagg atcatgaccg agagcaccag
      721 catggcggcc agcaggctgg tgcccttggg caggccgaag accctggcga tgaggggcac
      781 cagcaccgac acgccgatca ggccgtagac caccgagggg atgcccgcca gcagatccac
      841 cgccgggcgc acccggtccg ccagcttttt gggggccacc ttggcgagaa aaaccgcggt
      901 gagcaggccc accggcaccc cgatcagggt ggcgccggcg gtgccgtaga ccgaggtgag
      961 gataaagggg aggatgccga atttggggtc ttgcgcggtg gaggcccact cccgcccgaa
     1021 gagaaagttt ccaagcccga tctccctgag cgcgggcagg cccgccccca ccatgtaaac
     1081 ggtgatgagc agcacg
//
LOCUS       DBMDYA010000818         1093 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23833_length_1093_cov_1.469758, whole genome shotgun sequence.
ACCESSION   DBMDYA010000818 DBMDYA010000000
VERSION     DBMDYA010000818.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23833_length_1093_cov_1.469758"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(189..>1093)
                     /locus_tag="ACZPFK_14460"
     CDS             complement(189..>1093)
                     /locus_tag="ACZPFK_14460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1407560.1"
                     /translation="FEKMVVADRAAIFVNQVFARYRTCEGLTCVLAVVLFAIQVYCDF
                     AGYSDIACGAAEVMGFRLMQNFRQPYFAHTLAEFWARWHISLSTWLQDYLFEPLVWSG
                     WAQRLPLVGKRMKRPPVYSSLILTFLVSGLWHGASWTYVAWGALHGGLQVLGKLTKKL
                     RRRVLRRLRVEVDQPCHRLLQQMLVFTSVCASYVFFRSASIRDALNFFGRVFSTPNPW
                     VLFDGSLYCWGLDKLQWAVLLVAIAMLWLVDLWRGHADLRGQLERQCRPVRWTAYVGA
                     VLLVAVFGVYGPQFDASRFIYFQF"
BASE COUNT          260 a          395 c          284 g          154 t
ORIGIN      
        1 cctcgccggc ccggttcagc gggttctctt ccaccgcgca caggagattc gccgcaaggc
       61 gcatcgcgcc caccgacagc aacgcgaaca gcagcgcccg cgccgtgcag acaagacgcc
      121 ttttccaccg cctcgcctcc acgccggtcc ctcccctctt tttcaaaaac agcatggccg
      181 cgctcctctc aaaactgaaa atagatgaac cgggaggcgt caaactgcgg gccatagacc
      241 ccgaacaccg ccaccagcag caccgccccc acataggccg tccagcgcac cgggcggcac
      301 tgcctttcca gctgcccccg cagatccgca tgtccccgcc acagatccac cagccacagc
      361 atcgcgatcg ccaccagcag caccgcccat tgcagcttgt ccagccccca gcagtacagg
      421 gagccgtcaa agagcaccca gggattcggg gtgctgaaaa ccctgccaaa aaagttcagc
      481 gcatcccgaa tggaggccga gcgaaaaaat acatagctgg cgcagaccga ggtgaagacg
      541 agcatctgct gcaaaagccg gtggcagggt tgatccacct ccacccgcag gcgcctgagc
      601 acgcgccgac gcagcttttt cgttagcttg cccagcacct gcagcccccc gtgcagagcg
      661 ccccaagcca cataggtcca gcttgcccca tgccacagcc cactgaccaa aaaggtgagg
      721 atcagggagg aatagaccgg cggccgtttc atccgcttcc ccaccagggg cagcctttgg
      781 gcccagccgc tccacaccaa gggctcaaac agataatcct gcagccaggt ggagaggctg
      841 atatgccagc gggcccaaaa ctccgccagt gtgtgggcaa aatagggctg gcggaaattc
      901 tgcatcaggc ggaaccccat cacctcggcc gcaccgcagg cgatgtcgct gtacccggca
      961 aaatcacagt acacctgtat ggcaaacagc accaccgcca aaacgcaggt caggccctca
     1021 caggtccggt agcgtgcaaa cacctggttc acaaaaatcg cggcgcggtc ggcaacgacc
     1081 attttttcaa aat
//
LOCUS       DBMDYA010000819         1091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23897_length_1091_cov_1.038384, whole genome shotgun sequence.
ACCESSION   DBMDYA010000819 DBMDYA010000000
VERSION     DBMDYA010000819.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23897_length_1091_cov_1.038384"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /gene="coaBC"
                     /locus_tag="ACZPFK_14465"
     CDS             complement(<1..>1091)
                     /gene="coaBC"
                     /locus_tag="ACZPFK_14465"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1407561.1"
                     /translation="AAYKMANVASMLAKQHCDVQVIMTKNATQFITPLTFETLCNTRC
                     MVDTFDRNFQYNVEHVALAKRADLVMVAPASANIIAKMAHGIADDMLSTTVLACRCKK
                     LVAPAMNTNMYENPITQNNLETLRRYGFGIIDPAVGMLACKDVGAGKLPKEELLYNHI
                     LQEILYPKDLAGKKVLVTAGPTCEPVDPVRIYTNRSSGKMGYALARECSLRGADVTLV
                     TGKTSLEPWPFVKVVPIETAQQMFDEVTSRAPEMDIIIKAAAVADYRPKTVADQKLKK
                     GDGEFTLTFERTRDILKTLGERKPQGQFLCGFSMETENMLENSQAKLAKKNADMIVAN
                     NLKVAGAGFAGDTNVVTIITHDGVRELPL"
BASE COUNT          172 a          306 c          360 g          253 t
ORIGIN      
        1 atcagcggca gctccctgac accgtcatgg gtgatgatgg tgacgacatt ggtgtccccg
       61 gcaaagcccg cgcccgccac cttgaggttg ttggcgacga tcatgtcggc gtttttcttt
      121 gccagtttgg cctgcgagtt ttccagcatg ttttctgtct ccatcgagaa gccgcacaaa
      181 aactgcccct gcggcttgcg ctcacccagc gttttcagga tgtcccgggt gcgctcaaag
      241 gtgagagtaa attccccgtc gccctttttg agcttctggt ccgccaccgt cttggggcgg
      301 taatcggcta cggcggcggc cttgatgata atgtccatct ccggcgcgcg ggaagtgacc
      361 tcgtcaaaca tctgctgggc ggtctcgatg gggaccacct tgacaaacgg ccacggctcc
      421 aacgaggtct ttccggtgac cagggtgacg tctgcgccgc gcagcgagca ctcccgcgcc
      481 agggcgtagc ccatcttgcc gctggagcgg ttggtgtaga tgcgcaccgg gtcgaccggt
      541 tcacaggtgg gcccggcggt gaccagtacc tttttgccgg ccaaatcctt ggggtagagg
      601 atctcctgca ggatgtggtt gtacagcagc tcctcttttg gcagctttcc cgcgccgaca
      661 tccttgcagg ccagcatccc gactgccggg tcgatgatgc caaagccgta gcggcgcagc
      721 gtttccaggt tgttctgggt gatggggttt tcgtacatgt tggtgttcat cgcgggggcc
      781 accagctttt tgcagcggca ggccagcacg gtggtggaga gcatgtcgtc ggcgatgccg
      841 tgggccatct tggcgatgat gttggccgag gccggggcga ccatcacaag gtcggcgcgc
      901 ttggcaagcg ccacgtgctc gacgttgtac tggaagttgc ggtcgaaggt gtcgaccatg
      961 cagcgggtgt tgcatagcgt ctcaaaggtt agtggggtga tgaactgggt ggcgtttttg
     1021 gtcatgatga cctgcacgtc gcagtgctgt ttggccagca tgctggcgac gttggccatc
     1081 ttgtaggcgg c
//
LOCUS       DBMDYA010000820         1088 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23956_length_1088_cov_1.439716, whole genome shotgun sequence.
ACCESSION   DBMDYA010000820 DBMDYA010000000
VERSION     DBMDYA010000820.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23956_length_1088_cov_1.439716"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            14..127
                     /locus_tag="ACZPFK_14470"
     CDS             14..127
                     /locus_tag="ACZPFK_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019140.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407562.1"
                     /translation="MGYGQSEAALAIAKLDPALPVEELIKQALKSMAGKVR"
     gene            133..>1088
                     /gene="ruvB"
                     /locus_tag="ACZPFK_14475"
     CDS             133..>1088
                     /gene="ruvB"
                     /locus_tag="ACZPFK_14475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1407563.1"
                     /translation="MELSDDRLISPSPAAEDFDSDFTLRPRTLEEYVGQEKIKGNLRV
                     YLEAAKRRGEPLDHLLLYGPPGLGKTTLACIVAHEMGVQIRTTSGPAIEKPGDLAALL
                     TNLQEGDVLFIDEIHRLSRQVEEVLYPALEDYALDIMIGKGPSAQSIRINLPRFTLIG
                     ATTRAGQLTSPLRDRFGVLLKLELYSPDELARIVRRSAGLLDVPCDDAGAGELAKCSR
                     GTPRVANRLLKRVRDFAEVMGDGVITRDTAKVALKRMDIDELGLDELDRSLLRAVIEL
                     YGGGPVGLDTLAAALGEEAVTLEDVCEPYLMQMGFLARTPRGR"
BASE COUNT          176 a          363 c          385 g          164 t
ORIGIN      
        1 cgctctctgc tcgctgggct acggccagag cgaggcggcg ctggcgattg caaagctcga
       61 cccggcgctg ccggtggagg agctcatcaa acaggccctg aaatccatgg ccggaaaggt
      121 gcggtgaggt gaatggaact ctcggatgac cgtctgatct cgccctcccc ggcggcggag
      181 gattttgaca gcgatttcac gctgcgcccc cgcacgctgg aggagtatgt gggccaggag
      241 aagatcaagg gcaatctgcg ggtctatctc gaggccgcaa agcgccgcgg cgagccgctg
      301 gatcacctgc tgctctacgg cccccccggc ctgggcaaga cgacgctggc ctgcatcgtg
      361 gcccatgaga tgggggtgca gatccgcacc acctcgggcc cggccatcga aaagcccggc
      421 gacctggcgg ccctgctgac caatctgcag gagggggacg tgctcttcat cgacgagatc
      481 caccgcctct cccgccaggt ggaggaggtg ctctacccgg cgctggagga ctacgcgctg
      541 gacatcatga tcggcaaagg cccctcggcc caatccatcc gcatcaacct gccgcgcttc
      601 accctcatcg gggccaccac ccgggccggg cagctcacca gcccgctgcg cgaccgcttc
      661 ggcgtgctgc tcaagctgga gctctactcg ccggacgagc tggcccgtat cgtgcgccgc
      721 tcggccgggc tgctggatgt gccctgcgac gacgcgggcg cgggcgaact ggccaagtgc
      781 agccgcggca ccccgcgcgt ggcgaaccgc ctgctcaagc gggtgcgcga ctttgccgag
      841 gtgatggggg acggcgtcat cacacgggac accgcaaagg tggccctgaa gcgcatggac
      901 atcgacgaac tgggtctgga cgagctggac cgcagcctgc tgcgggcggt gatcgagctg
      961 tatggcggcg gcccggtggg gctggacacg ctggcggcgg ccctgggcga ggaggcggtg
     1021 acgctggagg acgtctgcga gccctatctg atgcagatgg gctttttggc ccgcaccccg
     1081 cgcggccg
//
LOCUS       DBMDYA010000821         1087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_23991_length_1087_cov_1.184584, whole genome shotgun sequence.
ACCESSION   DBMDYA010000821 DBMDYA010000000
VERSION     DBMDYA010000821.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23991_length_1087_cov_1.184584"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(290..991)
                     /locus_tag="ACZPFK_14480"
     CDS             complement(290..991)
                     /locus_tag="ACZPFK_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1407564.1"
                     /translation="MARVRLRMAEALRAAGRAEGDVLLCAASKMQDAATVRLAAGLEI
                     DLFGENRVQELCEKSGAAAYGGKPLHFIGHLQTNKVKLVVGSAQLIQSVGSEKLLRCI
                     AAQAEKAALIQDLLIEVNIGGEESKSGIAPVQLPALLEIARGLPAVRVRGLMAIPPAA
                     AAPGASRPYFARMRALFEASARQARDNISMEYLSMGMSGDFADAILEGANLVRVGTAI
                     FGPRPPMGQQQAPRA"
BASE COUNT          196 a          359 c          308 g          224 t
ORIGIN      
        1 catggccgtt tttacagctt tgcgttgcaa agctgtaaaa aaacaactta tttcaatggc
       61 cccgtttctc accgctgcgc ggcaaccgaa acggatggcc gaaaaatact ctccactctg
      121 ctgcggccat ttttttattg ctccgcaata aaaaacaact gatcctaatg tccgcgtttc
      181 tcgccgctgc gcggcaaccg aaacgaaagg ccgaaaaaat agcctccgct tcgctgcggc
      241 tactctttta tttatttaca caggtcttta ggcgggccgc aggcagggat caggcgcggg
      301 gcgcttgctg ctggcccatc ggcgggcggg ggccgaagat ggcggtgccc acccgcacga
      361 ggttggcgcc ctccagaatg gcgtccgcaa agtcgccgct catgcccata gagagatact
      421 ccatcgagat gttgtctctc gcctgccggg cggatgcctc gaacagcgcg cgcatacggg
      481 cgaaataggg ccggctggcc cccggcgcag cggcggcggg cgggatggcc atcaacccgc
      541 gcacccgcac tgcgggcagg ccgcgggcaa tctccagcag ggcgggcagc tggaccggcg
      601 caatgccgct cttgctctcc tcgccgccga tgttcacctc gatcagcagg tcctgtatca
      661 gggccgcctt ttccgcctgg gcggcgatgc agcgcagcag cttttccgac cccaccgact
      721 ggatgagctg cgcgctgccc accaccagct tcaccttgtt ggtctgcagg tggccgataa
      781 aatgcagcgg cttgccgccg taagcggcgg ccccgctttt ttcgcacagc tcctgtaccc
      841 ggttttctcc gaacaggtcg atctcgagcc cggccgccag ccgcacggtg gccgcatcct
      901 gcatcttgct ggcggcgcag agcagcacgt cgccctccgc acgcccggcg gcgcgcaggg
      961 cctcggccat gcgcagacgg acccgcgcca cattctcctg cagcagcttt tgcttttcgt
     1021 ttttttattc tttcagtaaa tcatccatca tggactgcaa atttgccatg tcacaatatt
     1081 ggttata
//
LOCUS       DBMDYA010000822         1083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24093_length_1083_cov_1.702648, whole genome shotgun sequence.
ACCESSION   DBMDYA010000822 DBMDYA010000000
VERSION     DBMDYA010000822.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24093_length_1083_cov_1.702648"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="ACZPFK_14485"
     CDS             complement(<1..750)
                     /locus_tag="ACZPFK_14485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR1407565.1"
                     /translation="MKKYVISLDQGTTSSRAIIFDRDQNIVGIAQKEFTQIYPKPGYV
                     EHDPMEIYASQYGVLIEVLAKSGISPDEVAAIGITNQRETTIVWDKKTGRPVYNAIVW
                     QCRRTADICEQMKRDGMEPYVKDTTGLIVDAYFSGTKIKWILDNVEGARERAEAGELL
                     FGTVDTWLIWKLTGGAVHVTDYTNASRTMLYDIHNLCWDKKICEYLGVPVQMLPQVCD
                     CSKVYGAVSIQGVEIPIAGIAGDQQAALFGQA"
BASE COUNT          194 a          328 c          307 g          254 t
ORIGIN      
        1 ggcctggccg aacagggccg cctgctggtc gcccgcgatg ccggcgatgg ggatctcgac
       61 gccctggatg ctgaccgcgc cgtaaacctt gctgcagtcg cacacctggg gcagcatctg
      121 cacgggcaca cccaggtact cgcagatttt tttgtcccag cacaggttat ggatgtcata
      181 gagcatggtg cgggaggcgt tggtgtaatc ggtgacgtgc accgcgccgc cggtcagctt
      241 ccagatcagc caagtgtcca cggtgccgaa cagcagctcg cccgcctcgg cccgctcccg
      301 cgcgccctcc acattgtcga ggatccattt gatcttggtg ccggagaaat aggcgtccac
      361 gatcaggccg gtggtatcct tcacataggg ctccatgccg tcgcgcttca tctgctcgca
      421 gatatcggcg gtgcggcggc actgccagac gatggcgttg tagaccggcc ggccggtctt
      481 tttgtcccac acgatggtgg tctcgcgctg gttggtgatg ccgatggccg ccacctcgtc
      541 cgggctgatg ccgctttttg ccagcacctc gatgagcacg ccgtattgcg aggcgtagat
      601 ctccatgggg tcgtgctcca catagcccgg cttggggtag atctgggtga attctttctg
      661 cgcgatgccc acgatgttct ggtcgcggtc aaagatgatg gcgcgcgagc tggtcgtgcc
      721 ctgatccaat gagatgacat actttttcat gcacaagcct cctgtttttc cgcctccgca
      781 gggaaacaaa aaagccaaag caaacagcct tgaaaaggcg tttttcgctt tggctctcgt
      841 ctctaagcgc aattcgtttt tctagctttt aatatagcat acaaaactga aaaccacaac
      901 caaccgtcat tcagcgtttt gcacaaatta ttcttttgtt ttttgtcatt gactttctga
      961 gagagggagg cgcctcctgc cgcggccccg ttcttccgcg tgcggggcgc ccctttccca
     1021 aaatagccgc aggctatttt tcggccatcc gcccagtccc ctggccgggg ccgtcaagat
     1081 cag
//
LOCUS       DBMDYA010000823         1083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24094_length_1083_cov_1.671079, whole genome shotgun sequence.
ACCESSION   DBMDYA010000823 DBMDYA010000000
VERSION     DBMDYA010000823.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24094_length_1083_cov_1.671079"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1083
                     /locus_tag="ACZPFK_14490"
     CDS             <1..>1083
                     /locus_tag="ACZPFK_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR1407566.1"
                     /translation="VIDIGGQDMKCFKVEHGAISDIYLNEACSSGCGSFLQTFANALG
                     YEVPEFARLGLFADRPADLGSRCTVFMNSSVKQAQKDGASVENISAGLSISVVKNALY
                     KVIRASSPEELGRRIVVQGGTFYNEAVLRAFEKEMGVEVVRPDIAGLMGAFGAALYGR
                     AKTAQDGRSGLLGAAALRNFTHQVKAVTCNGCGNHCQLTVNLFDGGRRFISGNRCEKP
                     VAGGKSGDELNMFEYKRRLLEGYRPVNGPRGRLGLPMGLNLYELLPFWHTFFTQLGFE
                     VVTSGASSRGLYLAGQGTIPSDTVCFPAKLLHGHIDRLCRMEGVDAIFYPCLTYNVNE
                     GLGDNHYNCPVVAYYTEVVAANCRCLA"
BASE COUNT          190 a          351 c          357 g          185 t
ORIGIN      
        1 cgtcatcgac atcggcggcc aggatatgaa gtgcttcaag gtcgagcacg gggccatcag
       61 cgatatctac ctgaacgagg cctgctcgtc gggctgcggc agctttttgc agacctttgc
      121 caacgccctg ggctacgagg tgcccgagtt tgccaggctg ggcctctttg ccgaccgccc
      181 ggcggacctg ggcagccgct gcaccgtgtt catgaactcc agcgtcaagc aggcccaaaa
      241 ggacggggcc tcggtggaga acatctcggc ggggctgtcc atcagcgtgg tgaaaaacgc
      301 gctgtacaag gtcatccgcg cctcctcgcc cgaagagctg ggccgccgca tcgtggtaca
      361 gggcggcacc ttttacaacg aggcggtgct gcgcgccttt gaaaaagaga tgggggtcga
      421 ggtggtgcgc cccgacatcg ccggcctgat gggcgctttt ggggccgccc tctacggccg
      481 ggccaagacg gcccaggacg gccgcagcgg cctgttgggc gcagcggcgc tgcggaactt
      541 cacccaccag gtgaaagccg tcacctgcaa cggctgcggc aaccactgcc agctcaccgt
      601 caacctgttc gacggcggcc gcaggttcat ttccggcaac cgctgcgaaa aaccggtggc
      661 ggggggcaag tccggcgacg agctgaatat gtttgaatat aagcgcagac tgctggaggg
      721 ctaccgcccg gtgaacggcc cccgcggcag gctggggctg cccatgggcc tgaacctgta
      781 cgagctgctg cccttttggc ataccttttt cacccagctg gggtttgagg tggtgaccag
      841 cggcgcctcc agccgggggc tgtacctggc cgggcagggc accatcccct cggacacggt
      901 ctgcttcccc gcaaagctgc tgcacggcca catcgatcgg ctgtgccgca tggagggggt
      961 ggacgccatc ttctacccct gcctcaccta caacgtaaac gaaggcctgg gcgacaacca
     1021 ctacaactgc ccggtggtgg cctattacac cgaggtggtg gcggccaact gccgctgcct
     1081 ggc
//
LOCUS       DBMDYA010000824         1080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24172_length_1080_cov_1.282942, whole genome shotgun sequence.
ACCESSION   DBMDYA010000824 DBMDYA010000000
VERSION     DBMDYA010000824.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24172_length_1080_cov_1.282942"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1080)
                     /locus_tag="ACZPFK_14495"
     CDS             complement(<1..>1080)
                     /locus_tag="ACZPFK_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1407567.1"
                     /translation="IVVIGGGGAGMTAAIQAVQDGATNVVIVEKMPQTGGNTTRSTGG
                     LNACETSYQAAEGIEDSVALFVEDTMKGGKELNNKELVTVMAENSAAAVDWVNDIGGD
                     LSVVGQFAGASVMRIHRPSDTSAVGPMLVKTLNAKVEELGIPVLLNTKAEEILTDETG
                     AVSGVRVTGPAGAYTIRCKAVVLATGGFGANNEMVAEYKPDLAGFGTTNQAGATGDGI
                     AMAKAVGADLVDMEQIQTHPTVNPDTQTMYTEGVRGNGAILVNKEGKRFVSEMETRDV
                     VSAAILQQTDGVCYMVFDQAVRDSLKAIESYITAGIVFESDTVEGLAEQLGMDPAALR
                     QTMDTYAGYAQAGVDEEFGRIDTSMG"
BASE COUNT          150 a          375 c          344 g          211 t
ORIGIN      
        1 gcccatggag gtgtcgatgc ggccgaattc ctcatccacg cccgcctgcg catagcccgc
       61 ataggtgtcc atggtctgcc gcagggcggc cgggtccatg ccgagctgct cggccaggcc
      121 ctccaccgta tcgctctcaa agacgatacc tgcggtgatg tagctctcga tggctttcag
      181 gctgtcgcgc accgcctggt caaagaccat atagcagacg ccgtcggtct gctgcaggat
      241 ggcggcggag accacgtcgc gggtctccat ctcgctgaca aagcgcttgc cctccttgtt
      301 caccaggatg gcgccgttgc cgcgcacgcc ttcggtgtac atggtctggg tgtccgggtt
      361 gacggtggga tgggtctgga tctgctccat gtccaccaga tcggcgccca cggccttggc
      421 catggcgatg ccgtcgccgg tggccccggc ctggttggtg gtgccgaagc cggccagatc
      481 gggcttgtac tcggccacca tctcgttgtt ggccccaaaa ccgccggtgg cgagcaccac
      541 ggccttgcat cggatggtgt aggcgccggc ggggccggtg acccgaacgc cgctgaccgc
      601 gcccgtttca tccgtcaaaa tctcctccgc cttggtgttc agcagcacgg ggatgcccag
      661 ctcctccacc ttggcgttca gggttttgac cagcatcggc cccacggcgg aggtgtcgct
      721 gggccggtgg atgcgcatca ccgacgcgcc cgcaaactgg cccaccaccg acaggtcgcc
      781 gccgatgtcg ttgacccaat ccaccgccgc ggcggagttt tcagccatca cggtgaccag
      841 ctctttgttg ttcagctcct tgccgccttt catcgtgtcc tcgacgaaca gcgccacgct
      901 gtcctcgatg ccctcggccg cctggtagga ggtctcgcag gcgttcaggc cgccggtgga
      961 gcgggtggta ttgccgccgg tctgcggcat cttctcgacg atcaccacgt tggtggcgcc
     1021 gtcctgcacc gcctggatgg cggcggtcat gcccgcgccg ccgccgccga tcacgacgat
//
LOCUS       DBMDYA010000825         1075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24325_length_1075_cov_1.205339, whole genome shotgun sequence.
ACCESSION   DBMDYA010000825 DBMDYA010000000
VERSION     DBMDYA010000825.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24325_length_1075_cov_1.205339"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZPFK_14500"
     CDS             complement(<1..207)
                     /locus_tag="ACZPFK_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HIR1407568.1"
                     /translation="MYNSIEDILSYVGENDVKFIRLAFCDIFGVQKNIAIQPSELPRA
                     FEKGISFDASAISGFMNVEESDLLL"
     gene            607..>1075
                     /locus_tag="ACZPFK_14505"
     CDS             607..>1075
                     /locus_tag="ACZPFK_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III domain-containing
                     protein"
                     /protein_id="HIR1407569.1"
                     /translation="MKERLPAETYAALKRTIEQGRSLDRGIADVVAGAMKDWAVEKGA
                     THFTHWFQPMTGITAEKHESFLCPQRDGTAILRFSGKELIRGEPDASSFPSGGLRATF
                     EARGYTAWDATSFAFIKDATLCIPTAFCSYGGEALDKKTPLLRSMELLNRQALR"
BASE COUNT          225 a          347 c          301 g          202 t
ORIGIN      
        1 caacagcagg tccgactcct ccacgttcat aaaaccgctg atcgccgaag cgtcgaaaga
       61 aatgcccttt tcaaaggcgc gcggcagctc tgagggctga atggcgatat ttttctgcac
      121 gccgaagatg tcgcagaacg ccagccggat gaatttgacg tcgttctccc ccacataact
      181 caggatgtcc tcaatgctgt tgtacatacc gcacctccat cattttgtcc ccggcggcgc
      241 gccccgcggc gtcgcccggg tgtggcgccc ggccgggctg aaaagccggg ctaaaaaaat
      301 caaggggtct cgtcaacaag cccccgcatc cgatgcggag gcccctgacg gacccctttg
      361 tccaccgcca gattatacaa tagcaggcgc ccccctgtca acgggaaaca caaaattttt
      421 tgcctgtttt tttgcggtcc gtcttgacaa atcatggtgt gtgggcatat aatgcggcct
      481 ataaacagca aaggcgctgt gggcatttcg gcccacggcg cctttctttt tgcccatcga
      541 gggaggatga gacaagtgaa cactgtgacg gaactctttg gaagcctggt cttcaacgag
      601 cgcgtgatga aagagcgcct acccgctgag acctacgcgg ccctcaagcg caccatcgag
      661 caggggcgca gcctggaccg cggcatcgcc gacgtggtgg cgggcgccat gaaagactgg
      721 gccgtcgaaa aaggcgccac ccatttcacc cactggttcc agcccatgac cggcatcacc
      781 gcggaaaagc acgaaagctt tctctgccca cagagagacg gcaccgccat cctgcgcttt
      841 tccggcaagg agctgatccg cggcgagccg gacgcctcca gctttccctc cggcgggctg
      901 cgcgccacct ttgaggcccg gggctacacc gcctgggacg ccacctcttt tgcctttatc
      961 aaagatgcga ccctgtgcat ccccaccgct ttctgctctt acggcgggga ggcgctggac
     1021 aaaaaaacgc cgctgctgcg cagcatggag ctgctgaacc ggcaggcgct gcgca
//
LOCUS       DBMDYA010000826         1066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24557_length_1066_cov_1.491192, whole genome shotgun sequence.
ACCESSION   DBMDYA010000826 DBMDYA010000000
VERSION     DBMDYA010000826.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24557_length_1066_cov_1.491192"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="ACZPFK_14510"
     CDS             <1..752
                     /locus_tag="ACZPFK_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1407570.1"
                     /translation="ARAVEQEAVMQGCALGFSYNASQTGELLALEPGGEEVGLVVLGR
                     PEDPHIIPELQGRFPHIVFTGLQRFDDPVDQVVADGRDAARTALDYLFSLGHREIGYI
                     GETVRELRYHGYLDFLRAKGLGYDQKFVADCICHGYDGGYQAALRLMSHAEKPTAIFC
                     ANDLTAFGVINALHSLGCRVPDEVSVISVDNVELAQFTTPMLTTIQIPRKEISHFAVK
                     LLLDKMEGVVAGRLTISLPCHLVVRNSCKKL"
BASE COUNT          171 a          351 c          380 g          164 t
ORIGIN      
        1 tggcccgcgc ggtggagcag gaggcggtga tgcagggctg cgcgctgggc ttttcttaca
       61 acgcctccca gaccggggag ctgctggcgc tggagccggg cggcgaggag gtggggctgg
      121 tggtgctggg ccgccccgag gacccgcaca tcatcccgga gctgcagggg cgcttccccc
      181 acatcgtctt caccgggctg cagcggttcg acgacccggt ggaccaggtg gtggccgacg
      241 gccgggacgc cgcccggacg gcccttgact atctcttttc cctggggcac cgggagatcg
      301 gctatatcgg cgagacggtg cgggagctgc gctatcacgg ctatctcgat tttttgcggg
      361 ccaaggggct aggctatgat caaaaatttg tggccgactg catctgccac ggctacgacg
      421 gcgggtatca ggcggcgctg cggctgatgt cccacgccga aaagcccacc gccatcttct
      481 gcgccaacga cctgacggcg ttcggcgtca tcaacgcgct ccactcgctg ggctgccggg
      541 tgccggacga ggtctcggtg atctcggtgg acaacgtgga gctggcccag ttcaccaccc
      601 cgatgctgac caccatccag atcccccgca aggagatcag ccatttcgcg gtcaagctgc
      661 tgctggacaa gatggagggc gtggtcgccg gcaggctgac catcagcctg ccctgccacc
      721 tggtggtgcg caacagctgc aaaaagctgt gagccgaagc gccggccccc ggcgttttga
      781 cgcgggcccc cggcggctgt gtaaaagatc gaaatagaca agaggagcgc ccccgggacg
      841 gcagttgaaa gccatcccgg gggcgctttt gctgaaaagg gccgcgtccg cacgaccagt
      901 gcccgggccc cgggcccaca ggcgtcaggg ggacgggtcc ggccccgggc cgtgcggaaa
      961 gacaggcccc gcgcggcagc ccgcagaggg gagacgccgc cgtgcccgcg cggcgcgcta
     1021 cggcgtttcg gcgtacaggc gcgccgcatc cgccgttccg ttgatg
//
LOCUS       DBMDYA010000827         1064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24611_length_1064_cov_1.940810, whole genome shotgun sequence.
ACCESSION   DBMDYA010000827 DBMDYA010000000
VERSION     DBMDYA010000827.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24611_length_1064_cov_1.940810"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="ACZPFK_14515"
     CDS             complement(<1..270)
                     /locus_tag="ACZPFK_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407571.1"
                     /translation="MEQREGYVVFIGGPCVDEYYSVGRWPHEGDKFVGRFAGNVPGGM
                     IANAACVFAGYGPKTYCFYGMGRGETTDFLLGDLEKHGVDTSRMIL"
     gene            complement(305..>1064)
                     /locus_tag="ACZPFK_14520"
     CDS             complement(305..>1064)
                     /locus_tag="ACZPFK_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1407572.1"
                     /translation="DALIEWSTYDRYYINHSGPTSKRAIALCKGEPVDDSQDYACCRN
                     AWATNGAGMKAWVAGVFNSGNLDKAIDDAILMCQVSHDNPVALSAACAVAAGVAAGMA
                     KDSTVADVVKAGLYGAREGYARAWRCARASAGASVEQRIRMAVTIGVNYAHDLDRLLQ
                     EMSDVVGTGLNANEAIPAAFGFFVAAQGRVMDGIYMGVNCGNDTDTVAAITGALCGAF
                     SGAGAIPPQHLPFLSEVNEMDIRQAVEEVDRVTG"
BASE COUNT          171 a          370 c          337 g          186 t
ORIGIN      
        1 caggatcatc cgggaggtat ccaccccgtg cttctccagg tcgcccagca aaaaatcggt
       61 ggtctccccc ctgcccatcc cgtaaaaaca gtaggtcttg gggccgtagc ccgcaaacac
      121 gcaggcggcg ttggcgatca tgccgcccgg cacgttgccc gcaaagcggc cgacgaattt
      181 gtccccctcg tggggccagc gccccacgct gtagtactcg tcgacgcacg gccccccgat
      241 gaacacgaca tagccctcgc gttgctccat ggcatttgtt cctcttttcg cagtttaccc
      301 gggttcagcc ggtgacccgg tcgacctcct ctaccgcctg ccggatatcc atctcgttga
      361 cctcgctcaa aaacggcaga tgctgcggcg ggatcgcccc cgcgccggaa aaagcgccgc
      421 agagcgcgcc ggtgatggcc gccaccgtgt cggtgtcgtt gccgcagttg acgcccatat
      481 agatgccgtc catcacccgg ccctgcgccg ccacaaaaaa gccgaaagcg gcggggatgg
      541 cctcgttggc gttgagtccg gtgcccacga cgtcgctcat ctcctgcagc agccggtcga
      601 gatcgtgcgc gtagttgacg ccgatggtca cagccatgcg gatgcgctgc tcgacgctgg
      661 cgcccgcgga ggcgcgcgcg cagcgccagg cgcgcgcata tccctcacgc gcgccgtaaa
      721 ggcccgcctt gaccacgtcg gccacggtgg agtcttttgc catgccggcc gcaacgccgg
      781 cggccaccgc acaggcggcg gagagcgcca ccgggttgtc gtgggagacc tggcacatca
      841 ggatggcgtc gtcgatggcc ttgtcgagat tgccgctgtt gaagacgccg gcgacccagg
      901 ctttcatccc cgccccattg gtggcccagg cgttgcggca gcaggcgtag tcctggctgt
      961 cgtccaccgg ctcgcccttg cacagcgcga tggcgcgctt gctggtgggg ccggagtggt
     1021 tgatgtaata gcggtcatag gtggaccact cgatcagcgc gtcg
//
LOCUS       DBMDYA010000828         1056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_24839_length_1056_cov_1.760209, whole genome shotgun sequence.
ACCESSION   DBMDYA010000828 DBMDYA010000000
VERSION     DBMDYA010000828.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24839_length_1056_cov_1.760209"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="ACZPFK_14525"
     CDS             <1..427
                     /locus_tag="ACZPFK_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR1407573.1"
                     /translation="MPGSAQEIRYINRDENTHLWLFRSILLELQKEQPELFTPVNIEA
                     LKGMLEEGVRQEIAWGSYVIGDEIPGLNSRMVTDYIRYLGNLRWSGLGYGYLYEDNRE
                     EPASMGWVSQYSNANMVKTDFFEAKSTAYAKSTALVDDL"
     gene            764..>1056
                     /gene="eno"
                     /locus_tag="ACZPFK_14530"
     CDS             764..>1056
                     /gene="eno"
                     /locus_tag="ACZPFK_14530"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537309.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1407574.1"
                     /translation="MKNFSIERVMGREIIDSRGNPTVEAEVFLAGGAIGRGAAPSGAS
                     TGEFEALELRDGDKGRFGGKGVLKAVENINTVINEALRGMDVCDIYAVDGAMRA"
BASE COUNT          251 a          262 c          324 g          219 t
ORIGIN      
        1 aatgcccggc tccgcacagg agatccgcta tatcaaccgg gacgaaaaca cccacctgtg
       61 gctgttccgc agcattttgc tggagctgca gaaagaacag ccggaactgt tcacccccgt
      121 caacatcgag gcgctgaaag ggatgctgga ggagggcgtg cgccaggaga tcgcctgggg
      181 cagctatgtg atcggggatg aaatcccggg cctcaacagc cgaatggtca ccgactatat
      241 ccgctatctc ggcaatctgc gctggtcggg cctgggatat ggatatctct atgaggacaa
      301 ccgggaagaa ccggccagta tggggtgggt gagccagtat tccaacgcga atatggtgaa
      361 aacagatttt tttgaggcca aaagcaccgc ctacgccaag agcactgcgc tggtggacga
      421 tctctgaaac gcccagtcgt ttttctacaa taaaaataag cgtctctcat tttcaaagga
      481 ttcaaaggac aggccgtatt gccgcggcct gtcctttttt atgtaccagg gcgttgatgg
      541 gcgactcaac agacggagca acgccccaag tgaagagcga gattttgcgc cgaagccttt
      601 ttctcccgcc tgatctgcca gcggcatggg ttgcatttaa aataaaaagg gattatactg
      661 tttttgcacg atgttagaag tgactaactg catgaaaatt gccatactac ttttataccg
      721 cggcgttccg gctgcggacg atcgggaaag gaagagggat tttatgaaaa acttttcaat
      781 tgagcgcgta atggggcgtg agatcatcga ctcccgcggt aacccaacgg tggaggccga
      841 ggtctttttg gctgggggcg caatcggcag gggggccgcc cccagcggcg cttccaccgg
      901 cgaatttgag gcgctggagc tgcgggacgg tgacaagggc cgctttggtg gaaagggcgt
      961 tttgaaagcg gtggagaaca tcaacaccgt gatcaacgag gcgctgcggg gcatggacgt
     1021 ctgcgacatc tacgcggtcg acggggccat gcgggc
//
LOCUS       DBMDYA010000829         1049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25045_length_1049_cov_1.190928, whole genome shotgun sequence.
ACCESSION   DBMDYA010000829 DBMDYA010000000
VERSION     DBMDYA010000829.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25045_length_1049_cov_1.190928"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            355..>1049
                     /locus_tag="ACZPFK_14535"
     CDS             355..>1049
                     /locus_tag="ACZPFK_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025802.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HIR1407575.1"
                     /translation="MQNYEGCSCPVCKKPFLPSDDIVVCPECGAPYHRACYQLKGACV
                     FEEMHGPGFEWKRPASAPKLGKCPNCGASYTGDLIFCEQCGAPLHTEEPRPAPGSALP
                     GMQGPVPLGAAEEMKGEIDGIPVADWASYIGPSAPYYLYQFKRMDVTGHKVGFVFSAA
                     FIAPIYFLYRKMWGLGILAGALNLLLNLPAALLMLAEFGIVFALPISYATLGVVASVC
                     SALLGALQIVGWG"
BASE COUNT          147 a          362 c          314 g          226 t
ORIGIN      
        1 aggtcgcggc ccgcacaggg caaaagccag gtctctctcg cctgcgcccc gcacgttgcc
       61 cccggcccgc gccgggggct gggccctcct ttgcctctct tctccccgct ctgcccgctc
      121 ttttgccacg tccggataga aaaatcgggt tcggcggcag ggttttcgcc cccggcgggc
      181 cgtctgcgcg ggcgtttgcg gcccgcttga cgctgcaatg tacccaatgt gccgttttcc
      241 caccctcttt cctctctcgg cacggcttta ccgcgccttt gcattttttg ccgctttgcg
      301 gtacaataac cactataagt attggaacta tcttcaccgt tggggaggct gtctatgcaa
      361 aattatgagg gttgcagctg cccggtctgc aagaaaccgt tcttgccgtc cgacgacatc
      421 gtcgtctgcc cggagtgcgg cgcgccctac caccgcgcct gttatcagct taaaggggcc
      481 tgtgtctttg aggagatgca cggccccggc tttgagtgga agcgcccggc cagcgcgccc
      541 aagctgggca aatgccccaa ctgcggcgca tcttataccg gcgatctgat cttttgcgag
      601 cagtgcggcg cgccgctgca caccgaggag cctcgccccg cgcccggatc cgccctgccc
      661 ggtatgcagg gccccgtccc gcttggcgcc gccgaggaga tgaaaggcga gatcgacggc
      721 atccccgtcg ccgactgggc ctcttacatc ggccccagcg ccccctatta cctctatcag
      781 ttcaagcgga tggacgtcac cggccacaag gtgggcttcg tgttcagcgc ggcgttcatc
      841 gcaccgatct attttcttta ccgcaagatg tggggccttg gcatcctcgc cggggcgctc
      901 aacctgttgc tcaacctgcc ggcggcgctg ctgatgctgg ccgagttcgg catcgtgttc
      961 gccctgccca tcagctacgc caccctgggc gtggtggcct cggtgtgctc ggccctgctg
     1021 ggggcattgc agatcgtggg ctggggcat
//
LOCUS       DBMDYA010000830         1041 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25291_length_1041_cov_2.039362, whole genome shotgun sequence.
ACCESSION   DBMDYA010000830 DBMDYA010000000
VERSION     DBMDYA010000830.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25291_length_1041_cov_2.039362"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(89..949)
                     /locus_tag="ACZPFK_14540"
     CDS             complement(89..949)
                     /locus_tag="ACZPFK_14540"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1407576.1"
                     /translation="MKTGLVLEGGALRTIFSSGACDALLDLGLTADYVVGVSAGIAYG
                     VSYLSGQKGRNLDILTTYANDRRYMGARNLLDRHNRSYFGLKFAYEEIPNKLIPFDYD
                     AFAAWPGEAEAVVTSLESGRADYLPVPRRDESFLLLQATCAMPLLFPTFDLGDGPVLD
                     GGVADAIPFRRALEKGCDRVTVILTRERGYHKKPEKLQRLIDLAYRRWPNFRAAMRSR
                     AQRYEAAREELFQLEREGRALILEPADTQGFSRVERDVEKIRALCREGYDAAMAKREE
                     LKAFLKSSAD"
BASE COUNT          158 a          387 c          318 g          178 t
ORIGIN      
        1 ttctgtttta tccagccgaa aagcaccggc ggcgcgcgtc ccgcgccctc ccgcccaaag
       61 agagcctccg ccgccccccg cctgcggctc agtccgcgct gctcttcaaa aacgctttca
      121 gctcctcccg ctttgccatg gcggcgtcgt agccctcgcg gcagagggcg cggatttttt
      181 ccacgtcccg ttccacccgc gaaaagccct gggtgtccgc aggctcgagg atcagggccc
      241 ggccctcccg ctccagctgg aacagctctt cccgcgcggc ctcataccgc tgggcgcggc
      301 tgcgcatggc cgcgcggaaa ttcggccagc ggcggtaagc gagatcgatc aggcgctgca
      361 gcttttccgg ctttttgtga tagccgcgct cccgggtgag gatgaccgtc acccggtcgc
      421 agcccttttc cagcgcccgg cgaaagggga tggcgtccgc caccccgccg tccagcaccg
      481 gcccgtcgcc caggtcaaag gtggggaaca gcagcggcat ggcgcaggtg gcctgcagca
      541 gcaaaaagct ctcgtcccgg cgcggcaccg gcaggtagtc cgcccggccg ctttccaggc
      601 tggtcaccac cgcctccgcc tcgccgggcc aggcggcaaa cgcgtcgtag tcgaacggga
      661 tcagcttgtt ggggatctcc tcataggcga atttcagccc gaaatagctg cggttgtgcc
      721 ggtccagcag gttgcgcgcc cccatgtacc gccggtcgtt ggcataggtg gtgaggatat
      781 ccaggttgcg ccctttctgc ccggacaggt agctgacccc gtaggcgatg ccggccgaca
      841 cccccaccac ataatccgcc gtcagcccca ggtccagcag ggcgtcgcag gccccgctgg
      901 aaaagatcgt gcgcagcgcg ccgccctcca ataccaatcc cgtcttcatg tcgtcttttc
      961 gtctcctttt tgcgtcccgt tgcaaaatac gggcggccgc cccggccggg ccgccccaaa
     1021 gcgcgcccgg ccctgaggcc g
//
LOCUS       DBMDYA010000831         1026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25772_length_1026_cov_1.429189, whole genome shotgun sequence.
ACCESSION   DBMDYA010000831 DBMDYA010000000
VERSION     DBMDYA010000831.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25772_length_1026_cov_1.429189"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="ACZPFK_14545"
     CDS             complement(<1..233)
                     /locus_tag="ACZPFK_14545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1407577.1"
                     /translation="MDLRLTFDEDAQNYDRARPGYPAELYDAVFAYCGAGPGARALEI
                     GPGTGQATAPFLEKGYRVTAVELGVQLSRYLEQ"
     gene            complement(262..390)
                     /locus_tag="ACZPFK_14550"
     CDS             complement(262..390)
                     /locus_tag="ACZPFK_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407578.1"
                     /translation="MLLDSFDGASRRLVTPADACGARERDLQFDCLCLIVALAATL"
     gene            complement(505..>1026)
                     /locus_tag="ACZPFK_14555"
     CDS             complement(505..>1026)
                     /locus_tag="ACZPFK_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR1407579.1"
                     /translation="LVRISPFDSSGRRHTSFASLEVMPEIDDTIEIDIRPEDIKMEVY
                     RSSGAGGQHINKTSSAVRLIHIPTGIVVACQNERSQFQNRDMAMKMLKSKLFQVKERE
                     HLAHIEDIKGEQLQIAWGSQIRSYVFMPYTLVKDHRTGYENGNVNAVMDGDLDGFINA
                     YLKATSRGELEGR"
BASE COUNT          169 a          329 c          314 g          214 t
ORIGIN      
        1 ttctgctcca gataccggct cagttgcacg cccagctcca ccgccgtcac ccggtacccc
       61 ttttccaaaa aaggggcggt cgcctgccct gtgccggggc cgatctccaa cgcccgggcg
      121 cccggcccgg cgccgcaata agcgaatacc gcgtcataca gctcggccgg atagccgggc
      181 cgcgcccggt cgtaattttg tgcgtcctca tcaaaggtca gccgcaggtc catcgcgctc
      241 tcctccctct cccactcgcc ctcaaagcgt ggccgccagc gccactatca ggcagaggca
      301 gtcgaattgc aggtcacgct cccgcgcgcc gcaggcatcc gcgggcgtga cgaggcggcg
      361 ggaggcgccg tcaaagctgt caaggagcat cgcttcctct tctttttttg caaaaacacc
      421 gcatcccccc gcccggctga gctttggctc agcctgataa gcgcagggtg cggtgtggtg
      481 attgtcgatc gtctttcggg gagattatct gccctccagc tcgccgcggc tggtggcttt
      541 aaggtaggcg ttgataaagc cgtccaggtc gccgtccatg acggcgttga cgttgccgtt
      601 ctcgtagccg gtgcggtgat ccttgaccag cgtgtagggc ataaagacat agctgcggat
      661 ctggctgccc caggcgatct ggagctgttc gcccttgatg tcctctatgt gggccagatg
      721 ctcccgttct ttgacctgga aaagcttgct ctttaacatc ttcatcgcca tgtcccggtt
      781 ctggaactgg ctgcgctcgt tctggcaggc gaccacgatg ccggtgggga tatggatcag
      841 gcgcacggcc gaagaggtct tgttgatgtg ctggccgcct gcgccggagg agcggtagac
      901 ctccatcttg atgtcctcgg gccggatgtc gatctcgatg gtgtcgtcga tctccggcat
      961 cacctccagc gaggcaaagc tggtgtgccg ccgcccggag gaatcaaagg gagagatgcg
     1021 caccag
//
LOCUS       DBMDYA010000832         1024 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25854_length_1024_cov_1.498375, whole genome shotgun sequence.
ACCESSION   DBMDYA010000832 DBMDYA010000000
VERSION     DBMDYA010000832.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_1024_cov_1.498375"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="ACZPFK_14560"
     CDS             complement(<1..666)
                     /locus_tag="ACZPFK_14560"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1407580.1"
                     /translation="MDKRAEALEAHKKWQGKIEVTSRARLETMADLAVAYTPGVAEPC
                     KEIERDEDLSYIYTRRGNLVAVVTDGTAVLGLGDIGPAAGMPVMEGKCVLFKEFGGVD
                     AFPICIDSKDPDEIVRTVQLISKSFGGINLEDIAAPRCFEIERRLQEVCDVPVFHDDQ
                     HGTAIVLSAALINALKVVGKPEGGVRVVINGAGAAGISIARLILQMKLGSVTLVDRFG
                     ILYD"
BASE COUNT          167 a          371 c          289 g          197 t
ORIGIN      
        1 gtcatagagg atgccgaagc ggtccaccag cgtcacgctg cccaacttca tctgtaaaat
       61 cagccgggcg atggagatgc cggccgcgcc cgcgccgttg atgaccaccc gcacgccgcc
      121 ctcgggcttg cccaccacct tgagcgcatt gatgagggcc gccgagagca cgatggcggt
      181 gccgtgctgg tcgtcgtgaa agacagggac gtcgcagacc tcctgcagcc gccgctcgat
      241 ctcaaagcag cgcggggccg cgatgtcctc caggttgatg ccgccaaagc tcttggagat
      301 cagctgcacg gtgcgcacga tctcgtccgg gtccttggaa tcgatgcaga tagggaaagc
      361 gtccacaccg ccgaactcct tgaagagcac acatttgccc tccatgaccg gcatgcccgc
      421 cgccgggccg atatccccca gccccagcac cgccgtgccg tcggtgacca ccgccaccag
      481 gttgcccctg cgggtataga tgtagctcaa atcctcgtcc ctttcgatct ccttgcaggg
      541 ctcggccacc ccgggcgtat aggccaccgc cagatcggcc atggtctcca gcctggccct
      601 gctggtcacc tcgatcttgc cctgccattt tttgtgcgcc tccagcgcct cggcgcgctt
      661 gtccagctcc tgtgccatct ccaacaactt cctttcaggc ctttccgccc gtggatttcc
      721 tgcctcccat tatagcaaat gccgccgtct ctgcaaagcc tttcgccgcc cgggcaggga
      781 caaaagccgc gcggcccggc gccccgcgca aaccgccctt tgcgcggcgg ggccccggag
      841 ccccgctgcg gcggtgtttt gacggcggcc gctttaagaa aatcttaggg atttctatac
      901 ctctttttta aagatcctgg gatatcttag aaaagaggcc cggcccgacg gcttgtcccc
      961 tttcgcctcg cgcgggcgtc ggttccggcc gcaccggccc gccctgcttt tgaaaccggg
     1021 cccg
//
LOCUS       DBMDYA010000833         1023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25889_length_1023_cov_1.321041, whole genome shotgun sequence.
ACCESSION   DBMDYA010000833 DBMDYA010000000
VERSION     DBMDYA010000833.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25889_length_1023_cov_1.321041"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1023)
                     /locus_tag="ACZPFK_14565"
     CDS             complement(<1..>1023)
                     /locus_tag="ACZPFK_14565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1407581.1"
                     /translation="RSTTSILDAANKVIKNNVGRKGKTLWTDNGDGDRVCLYRADNEQ
                     DEAAHIASVIGQNLKNGAHLRDHAVLYRMNAQSGPIETYFARAGIPYKIVGGHRFFDR
                     KEVKDIISYLSVVANPRDDLRLRRIINEPARKIGAATLDTAAGIAAGLGCGLMEVVAE
                     AQNYPALGRALVPLKNFCDIYDDLVAAAEEQPLDELVSTVIDRSGYRQMLEAQGEEGR
                     TRLENLGELVSSVKTYADEKGDEASLEGYLEEVALISDIDGYDEEADTVVMMTMHAAK
                     GLEFDYVFLVGLEEGVFPGEMSRYSDEDLEEERRLCYVGITRAKKELYLSCARTRMIF
                     GQTKRNP"
BASE COUNT          149 a          346 c          325 g          203 t
ORIGIN      
        1 gggttgcgct tggtctggcc gaagatcatg cgggtgcgcg cgcaggagag gtagagctct
       61 ttttttgccc gggtgatgcc cacatagcac aggcggcgct cctcctccag atcctcgtcg
      121 gagtagcggc tcatctcgcc ggggaagacc ccctcctcca gccccaccag gaacacgtaa
      181 tcgaattcaa gccccttggc cgcgtgcatg gtcatcatga ccacggtgtc ggcctcctcg
      241 tcgtagccgt cgatgtccga gatcagggcg acctcctcca gatagccctc caggctggcc
      301 tcgtccccct tttcatccgc gtaggtcttc accgaggaga ccagctcgcc caggttttcc
      361 agccgggtgc gcccctcctc cccctgggcc tccagcatct gccggtagcc gctgcggtcg
      421 atgacggtgg agaccagctc gtccagcggc tgctcctcgg ccgcggccac cagatcgtcg
      481 tagatgtcgc agaagttctt cagcggcacc agcgcccggc ccagagcggg gtaattctgc
      541 gcctcggcga cgacctccat caggccgcag cccagcccgg cggcgatccc tgccgccgtg
      601 tccagcgtgg ctgccccgat cttgcgcgcg ggctcgttga tgatgcggcg caggcgcaaa
      661 tcgtcgcgcg ggtttgccac caccgacaga taggagatga tgtccttgac ctccttgcgg
      721 tcgaaaaagc ggtggccgcc cacgatcttg taggggatgc cggcacgggc gaaataggtc
      781 tcgatggggc cggactgggc gttcatgcgg tagagcaccg cgtggtcgcg cagatgcgcc
      841 ccgtttttca ggttctggcc gatgaccgag gcgatatggg ccgcctcgtc ctgctcgttg
      901 tcggcgcggt aaaggcagac cctgtcgccg tccccgttgt cggtccacag cgtcttgccc
      961 ttgcgcccca cgttgttttt gatgaccttg ttcgccgcgt ccaggatgct ggtggtggag
     1021 cgg
//
LOCUS       DBMDYA010000834         1022 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25918_length_1022_cov_1.747014, whole genome shotgun sequence.
ACCESSION   DBMDYA010000834 DBMDYA010000000
VERSION     DBMDYA010000834.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25918_length_1022_cov_1.747014"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="ACZPFK_14570"
     CDS             complement(<1..387)
                     /locus_tag="ACZPFK_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407582.1"
                     /translation="MEQNVKLLEAIVKCGEMGRGTLDHMSDINENGAFAASMQIQQNE
                     YEAIREEAARQLAAMGRSVEQLSVMEKLNTAMGVKMNTLADKSARHMAEMLIQGSTMG
                     IVDLTKAMRDNPGAGAEVQALSDRMVQ"
     gene            complement(730..>1022)
                     /locus_tag="ACZPFK_14575"
     CDS             complement(730..>1022)
                     /locus_tag="ACZPFK_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1407583.1"
                     /translation="FDGITSLSVKPIRMITGLGVVVSVLSFIGVLWAIIAALSGHAVA
                     GWASTVCMVCFMGGVQLLCLGVIGEYIGKIYMETKARPRYIISEHTWDGKEK"
BASE COUNT          179 a          345 c          305 g          193 t
ORIGIN      
        1 ctgaaccatg cggtcggaca gcgcctgtac ctcggccccg gcgccggggt tgtcccgcat
       61 cgccttggtc aggtccacga tgcccatggt gctgccctgg atgagcattt ccgccatgtg
      121 gcgggcgctc ttgtcggcca gcgtgttcat cttgaccccc atggcggtgt tcagcttctc
      181 catcaccgag agctgctcca cgctgcggcc catggcggcc agctggcgcg cggcctcctc
      241 gcggatggcc tcgtactcgt tctgctggat ctgcatcgag gcggcgaaag cgccgttttc
      301 attgatgtcg ctcatatggt ccagcgtgcc ccggcccatc tcgccgcatt tgacgatggc
      361 ctccaacaat tttacatttt gttccataaa aaaagccccc tttctgcgct tagtctggac
      421 agaaaaaagg aggctattct tttttgcgct gttttgaggc gaaaggcctc gttctcccgc
      481 cgggaacggc agttccccca ccagaggaag caaaacggtg agagcaaaat gcccgcgagc
      541 ggggcggcgc ggcggccctg cgggcgtctc tccttccccc gcgcacacaa aaagaggtaa
      601 tcctgcgacc cgcgccctct cccccggcac aactgcgcgg gggcggccgc gtcggcaggc
      661 gcagcgcccc gcgctgcggc ccctattttt cctccggcag gcgcgccgaa aaagagggag
      721 ggaaacccgt tatttctcct tgccgtccca ggtatgctcg ctgatgatat agcgggggcg
      781 ggccttggtc tccatataga tcttgccgat gtactcgccg atcacgccca gacagagcag
      841 ctgcacgccg cccataaagc agaccatgca gacggtgctg gcccagcccg ccaccgcgtg
      901 gccgctgagg gcggcgatga tggcccagag caccccgata aagctgagca ccgagaccac
      961 cacccccagg ccggtgatca tgcggatcgg cttcaccgac aggctggtga tgccgtcaaa
     1021 gg
//
LOCUS       DBMDYA010000835         1021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_25969_length_1021_cov_1.148913, whole genome shotgun sequence.
ACCESSION   DBMDYA010000835 DBMDYA010000000
VERSION     DBMDYA010000835.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25969_length_1021_cov_1.148913"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(35..871)
                     /gene="prmC"
                     /locus_tag="ACZPFK_14580"
     CDS             complement(35..871)
                     /gene="prmC"
                     /locus_tag="ACZPFK_14580"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1407584.1"
                     /translation="MVAAEAYRAARDALRQAGIEDAAFEADELLRLVTGRGHLQQEAA
                     APLKEEQAQRFQTLLRRRCERYPLQYLAGHWPFYGLELAVGEGVLIPRPDTETVVEQV
                     LKLLKEVPAPRVLDLCAGSGAIGLAVKQMRPDARVTLVERSQAALRYCRENAGQRARV
                     LEADVFGCERALQAEHFDCIVANPPYVTEAEYARLEPELLFEPREALVARDEGLAFYA
                     YIAPCYRDRLVPGGALVFEIGATQRAAVQAILARSGYEKIDGERDLGGNDRCVWGRRP
                     VE"
     gene            complement(865..>1021)
                     /locus_tag="ACZPFK_14585"
     CDS             complement(865..>1021)
                     /locus_tag="ACZPFK_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1407585.1"
                     /translation="GRHDNPVTRVISAPGLWLQRLTTAEPDDSMIEVAIASVQAVLPK
                     EGEDDRW"
BASE COUNT          151 a          391 c          325 g          154 t
ORIGIN      
        1 agcgccgtgg caaggtcgtc cccggcccca cgcctcattc cacagggcgg cgcccccaca
       61 cacagcggtc gttgccgccg agatcccgct cgccgtcgat tttttcatat ccgctgcggg
      121 ccaaaatcgc ctgcaccgcc gcccgctggg tggcgccgat ctcgaagacc agcgcgccgc
      181 ccgggaccag ccggtcccga taacagggcg cgatataggc gtaaaacgca aggccctcgt
      241 cccgcgccac caacgcctcc cgcggctcga acaaaagctc cggctcaagg cgggcgtatt
      301 ccgcctcggt gacgtaaggg gggttcgcga cgatgcagtc aaagtgctcc gcctgcagcg
      361 ctcgctcgca gccgaagacg tcggcctcca gcacccgggc cctttgccct gcgttttcgc
      421 ggcaatagcg cagcgcggcc tgcgagcgct ccaccagcgt gacccgggcg tccggccgca
      481 tctgtttgac ggccagcccg atggcgccgc tgcccgcgca gaggtccagc acccgggggg
      541 ccggtacctc tttcagcagc ttcagcacct gctccaccac cgtctcagtg tcggggcgcg
      601 ggatgaggac cccctccccc accgccagct ccaggccgta aaagggccag tgcccggcca
      661 gatattgcag cggataccgc tcgcagcggc ggcgcagcag cgtttgaaag cgctgcgcct
      721 gctcttcttt cagcggcgcg gcggcctcct gctgcaggtg gcccctgccg gtgacgagcc
      781 gcagcagttc gtccgcctca aaggcggcgt cctcgatgcc cgcctgccgc agcgcatcgc
      841 gggcggcccg gtaagcctcc gccgctacca acggtcgtcc tctccctctt tgggcagcac
      901 cgcctgcacc gaggcgatgg ccacctcgat catcgagtcg tccggctctg cggtggtcag
      961 ccgctgcagc caaaggcccg gggcggagat gacccgggtg accgggttgt cgtggcggcc
     1021 c
//
LOCUS       DBMDYA010000836         1015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_26143_length_1015_cov_1.242888, whole genome shotgun sequence.
ACCESSION   DBMDYA010000836 DBMDYA010000000
VERSION     DBMDYA010000836.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26143_length_1015_cov_1.242888"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="ACZPFK_14590"
     CDS             complement(<1..599)
                     /locus_tag="ACZPFK_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HIR1407586.1"
                     /translation="MKQQRPYSRYTVSRKAQASIENGHPWVYEDEIRAVEGEAENGAL
                     VDVLSDKGRYLGTGFLSAHSKIRVRLVSRNANDSFDEAFWRRRLQWAWAYRKQVLAPA
                     DLGCCRVIFGEADQLPGLTVDRFGPYLVAQTLSVGIERLKGLLFPLLAQVLRDDGQQI
                     AGLYERNDLALREREGLEQGKGFFPLPGLPVPAVTETII"
BASE COUNT          161 a          312 c          398 g          144 t
ORIGIN      
        1 cagatgattg tctcggtgac ggcgggcacg ggcaggcccg gcagcggaaa aaagcccttg
       61 ccctgctcca gcccctcccg ctcgcgcagg gccaggtcgt tgcgctcgta aaggcccgcg
      121 atctgctgcc cgtcgtcgcg cagcacctgt gccagcagcg ggaagagcag ccctttcagc
      181 cgctcgatgc ccaccgagag ggtctgggcg accagatagg ggccgaagcg gtccaccgtg
      241 aggccgggca gctggtccgc ctcgccgaag atcacgcggc agcagcccag atcggcgggg
      301 gccagcacct gtttgcggta ggcccaggcc cactgcaggc ggcggcgcca gaaagcctcg
      361 tcaaagctgt cgttggcgtt gcggctgacc agccgcaccc tgatcttgct gtgggcgctg
      421 agaaagccgg tgccgagata ccggcccttg tcgctgagaa catccaccag agcgccgttt
      481 tccgcctcgc cctccacggc gcggatctcg tcctcataca cccaggggtg tccgttttcg
      541 atggaggcct gggccttgcg gctgaccgtg taacgggaat agggacgctg ctgcttcaag
      601 tgggtcactc ctctttaagg tgtctggcgt cggcgcgccc gtgggcggcc gcacaccgat
      661 tataccccgc ccgggcccgg gggtcaaccg ggaggcgccg ggggaggcgg acggagggcg
      721 taggaaaacg cgccgctgcg ggcggaggcg cggcgctggg aaagcgtgaa acaagggaag
      781 ctcccgcatg ggtggcggac ggtcttgggc gaggtgaggg ccaggggcgc gcgggccgcg
      841 tttttccggg ccggggggag gcgaggcggc ggcggaaagc agggcggctt aacggccgga
      901 gggatagcgg gcaaagcggc gggacggcga agccgcgccg gggggatgcg gccggggcgt
      961 gggggaatgg gcgaacggtc ggaggctgaa acgaccgggc ggctttgatc tcttg
//
LOCUS       DBMDYA010000837         1013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_26206_length_1013_cov_0.976974, whole genome shotgun sequence.
ACCESSION   DBMDYA010000837 DBMDYA010000000
VERSION     DBMDYA010000837.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26206_length_1013_cov_0.976974"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="ACZPFK_14595"
     CDS             <1..242
                     /locus_tag="ACZPFK_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1407587.1"
                     /translation="SNKAAAKIREDFTLSGARYRYTSSYSRFSLFSFFYSDIRNLPPP
                     FLHENTFFDAAFSAPRPRELAGLSAHQLQKRPFPL"
     gene            complement(268..>1013)
                     /locus_tag="ACZPFK_14600"
     CDS             complement(268..>1013)
                     /locus_tag="ACZPFK_14600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1407588.1"
                     /translation="IKIRTFADETVSKLDYAVNALTQQGATSLIFDVRGNTGGSLSAA
                     AECVDLLCPAGTIVSGAYKGGETKVLYTSDEKEVDLPMVVLTNGSTASGAELFAVSVR
                     DFGKGRIVGAKTAGKGTLQRMYTMTDGSAVSLTVAVMVPGKSESFDGVGVAPDYERVL
                     SAEEELAYYDYTVNDDPQIQRAIEVAVSLAAGNSGNGPAPSSSAAPGSSSQASSSEAG
                     AGSSAPQDGSSAAQDGSAPAESSSSDSAA"
BASE COUNT          156 a          335 c          322 g          200 t
ORIGIN      
        1 ggtcaaacaa agctgccgcc aaaatccggg aggatttcac cctctcgggg gctcggtata
       61 gatatacaag ctcatattca cgattttcgt tgttctcttt cttttactcg gatatccgca
      121 atttgccgcc gccgtttttg catgaaaata catttttcga cgcggcgttt tcggctcccc
      181 gcccccgtga gctcgccggg ttgtccgcgc atcaactaca aaaaaggcct ttcccgctgt
      241 agccgggaaa ggccctggtc ttttttgtca tgccgcgctg tcggaagaag agctctctgc
      301 gggcgcggag ccgtcctgcg cggcggagga gccgtcctgc ggcgcggagg agcccgcccc
      361 cgcctcgctg gaggaggcct gggaggaaga gcccggcgcg gcgctggacg agggggcggg
      421 gccgttgccg ctgttgcccg ccgccaggct caccgccacc tcgatggccc gctggatctg
      481 cgggtcgtcg ttcaccgtgt agtcgtaata ggccagctcc tcctcggcgg agaggacgcg
      541 ctcatagtcg ggcgccacgc ccacgccgtc aaagctctcg ctcttgcccg gcaccatcac
      601 cgccaccgtc aggctgacgg cgctgccgtc ggtcatggtg tacatccgct gcagggtgcc
      661 cttgcccgct gttttggcgc ccacgatgcg ccccttgccg aaatcgcgca ccgagaccgc
      721 aaagagctcg gcgccggagg cggtgctgcc gttggtgagc accaccatgg gcaggtcgac
      781 ctctttttcg tcgctggtgt acagcacctt ggtctcgccg cccttatagg cgcccgagac
      841 gatggtgccc gccgggcaga gcaggtccac gcactcggcc gccgccgaca gcgagccgcc
      901 ggtgttgccg cgcacgtcga agatcagcga ggtcgcgccc tgctgggtga gggcgttgac
      961 ggcatagtcc agcttggaca ccgtctcgtc ggcaaaggtg cgtatcttga tat
//
LOCUS       DBMDYA010000838         1001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Pygmaiobacter sp. isolate MAG1164
            NODE_26609_length_1001_cov_1.890000, whole genome shotgun sequence.
ACCESSION   DBMDYA010000838 DBMDYA010000000
VERSION     DBMDYA010000838.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508263
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pygmaiobacter sp. (human gut metagenome)
  ORGANISM  Pygmaiobacter sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Pygmaiobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.4836x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:32:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 5 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Pygmaiobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26609_length_1001_cov_1.890000"
                     /isolate="MAG1164"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1929306"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(123..992)
                     /locus_tag="ACZPFK_14605"
     CDS             complement(123..992)
                     /locus_tag="ACZPFK_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1407589.1"
                     /translation="MLRSSGNSTAAPARLAWLFLAPSLAGLLLFVLFPFADTVRRSFF
                     DPMGGSFVGLENYRSVVANGAFRLAAKNTARFVAVCLPLLLALSLALALLVRAAKGGG
                     RLFKTTYLIPMAVPVASIALLWQALFAQNGLANALLTALGGRPVDFMGTGAAFWVLIF
                     TYLWKNSGYDMILWMAGLDGISESLYEAARVDGASNFQCFRYITLPGLLPTLFMTAVL
                     SLLNTFKVFREAYLVAGSYPQNDIYLLQHLFNNWFADLDLGRLSAAAVLVAAVLLAAI
                     LLFQRFLNRGDEI"
BASE COUNT          225 a          336 c          304 g          136 t
ORIGIN      
        1 caggggcatc agcgccccct ccaccatgaa ccagagcggc caccacacca gcagggccag
       61 ccccgacagc aggcagaaga gaaaaaggcg cgagagcgct ttggagcgtt tgcccgggcg
      121 gctcatatct catcccccct gttgaggaag cgctggaaca gcaggatggc cgccagcagc
      181 accgcggcca ccagcaccgc agccgccgaa agccggccca ggtccaggtc tgcaaaccag
      241 ttgttgaaca gatgctgcag cagatagatg tcgttttgcg gatagctgcc cgccaccaga
      301 tacgcctccc gaaagacctt gaacgtgttc agcaggctga gcaccgccgt catgaacagg
      361 gtcggcagca gcccgggcag cgtgatgtag cggaagcatt gaaaattcga cgctccatcc
      421 acccgggccg cctcgtaaag gctctccgag atgccgtcca gccccgccat ccaaaggatc
      481 atgtcgtagc cgctgttctt ccacagatag gtgaagatca gcacccaaaa agccgcgccg
      541 gtgcccataa aatccaccgg ccggccgccc agtgcggtca gcagcgcgtt ggcgaggccg
      601 ttttgggcaa agagcgcctg ccacagcaaa gcgatgctcg ccaccggcac cgccatgggg
      661 atgagatagg tggttttgaa cagcctgccg cccccctttg ccgcccgcac cagcagcgcc
      721 agcgccaggc tgagagccag cagcaggggc aggcagaccg cgacgaaccg cgcggtgttt
      781 tttgccgcca gccggaaagc gccgttcgcc accaccgacc tgtaattttc aagccccaca
      841 aagctgccgc ccatggggtc gaaaaagctg cgccgcaccg tgtccgcaaa ggggaaaagc
      901 acgaacagca gcagcccggc aaggcttggg gcgagaaaga gccacgccag ccgggccggg
      961 gcggcggtgc tattgccgct gctcctcaaa atagaggctt g
//
